LOCUS       DBERCA010000001       110533 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.001, whole genome shotgun sequence.
ACCESSION   DBERCA010000001 DBERCA010000000
VERSION     DBERCA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 110533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.001"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..930
                     /locus_tag="ACL719_000001"
                     /pseudo
     CDS             1..930
                     /locus_tag="ACL719_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1325..2188
                     /locus_tag="ACL719_000002"
     CDS             1325..2188
                     /locus_tag="ACL719_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS0698058.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVTCAGLTTSQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRFGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKLLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            2474..2707
                     /locus_tag="ACL719_000003"
     CDS             2474..2707
                     /locus_tag="ACL719_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698059.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            2729..3406
                     /locus_tag="ACL719_000004"
     CDS             2729..3406
                     /locus_tag="ACL719_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0698060.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWFYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            3418..4083
                     /locus_tag="ACL719_000005"
     CDS             3418..4083
                     /locus_tag="ACL719_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0698061.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTVLEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            4155..5381
                     /locus_tag="ACL719_000006"
     CDS             4155..5381
                     /locus_tag="ACL719_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0698062.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            5643..6299
                     /locus_tag="ACL719_000007"
     CDS             5643..6299
                     /locus_tag="ACL719_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGS0698063.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            6289..6612
                     /locus_tag="ACL719_000008"
     CDS             6289..6612
                     /locus_tag="ACL719_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGS0698064.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            6716..7546
                     /locus_tag="ACL719_000009"
     CDS             6716..7546
                     /locus_tag="ACL719_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698065.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7700..8554
                     /locus_tag="ACL719_000010"
     CDS             7700..8554
                     /locus_tag="ACL719_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS0698066.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8654..10027
                     /locus_tag="ACL719_000011"
     CDS             8654..10027
                     /locus_tag="ACL719_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGS0698067.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVSMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            10020..11081
                     /locus_tag="ACL719_000012"
     CDS             10020..11081
                     /locus_tag="ACL719_000012"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGS0698068.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            11083..11775
                     /locus_tag="ACL719_000013"
     CDS             11083..11775
                     /locus_tag="ACL719_000013"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGS0698069.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11805..12374)
                     /locus_tag="ACL719_000014"
     CDS             complement(11805..12374)
                     /locus_tag="ACL719_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS0698070.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVTPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12509..13123
                     /locus_tag="ACL719_000015"
     CDS             12509..13123
                     /locus_tag="ACL719_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS0698071.1"
                     /translation="MGRFLDFVFNRFFLGMIATALFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            13101..14771
                     /locus_tag="ACL719_000016"
     CDS             13101..14771
                     /locus_tag="ACL719_000016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0698072.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HIGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14783..16069
                     /locus_tag="ACL719_000017"
     CDS             14783..16069
                     /locus_tag="ACL719_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS0698073.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            16121..16654
                     /locus_tag="ACL719_000018"
     CDS             16121..16654
                     /locus_tag="ACL719_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698074.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            16732..17202
                     /gene="nrdI"
                     /locus_tag="ACL719_000019"
     CDS             16732..17202
                     /gene="nrdI"
                     /locus_tag="ACL719_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGS0698075.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17495..18757)
                     /locus_tag="ACL719_000020"
     CDS             complement(17495..18757)
                     /locus_tag="ACL719_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGS0698076.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIEKAG"
     gene            complement(19067..19525)
                     /locus_tag="ACL719_000021"
     CDS             complement(19067..19525)
                     /locus_tag="ACL719_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGS0698077.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19522..19962)
                     /locus_tag="ACL719_000022"
     CDS             complement(19522..19962)
                     /locus_tag="ACL719_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0698078.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            20318..22267
                     /gene="mutL"
                     /locus_tag="ACL719_000023"
     CDS             20318..22267
                     /gene="mutL"
                     /locus_tag="ACL719_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGS0698079.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVDQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(22592..23059)
                     /gene="ribE"
                     /locus_tag="ACL719_000024"
     CDS             complement(22592..23059)
                     /gene="ribE"
                     /locus_tag="ACL719_000024"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGS0698080.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(23060..24265)
                     /locus_tag="ACL719_000025"
     CDS             complement(23060..24265)
                     /locus_tag="ACL719_000025"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGS0698081.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRIDDLIQFSKKHNIPLITIKELQEYRKAYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKIIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(24285..24920)
                     /gene="ribE"
                     /locus_tag="ACL719_000026"
     CDS             complement(24285..24920)
                     /gene="ribE"
                     /locus_tag="ACL719_000026"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGS0698082.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(24905..26005)
                     /gene="ribD"
                     /locus_tag="ACL719_000027"
     CDS             complement(24905..26005)
                     /gene="ribD"
                     /locus_tag="ACL719_000027"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGS0698083.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(26139..26253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26410..27003
                     /gene="ruvA"
                     /locus_tag="ACL719_000028"
     CDS             26410..27003
                     /gene="ruvA"
                     /locus_tag="ACL719_000028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGS0698084.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            27013..27576
                     /locus_tag="ACL719_000029"
     CDS             27013..27576
                     /locus_tag="ACL719_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGS0698085.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            27573..28250
                     /locus_tag="ACL719_000030"
     CDS             27573..28250
                     /locus_tag="ACL719_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0698086.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(28387..29418)
                     /locus_tag="ACL719_000031"
     CDS             complement(28387..29418)
                     /locus_tag="ACL719_000031"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGS0698087.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(29673..30356)
                     /locus_tag="ACL719_000032"
     CDS             complement(29673..30356)
                     /locus_tag="ACL719_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGS0698088.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(30368..31312)
                     /locus_tag="ACL719_000033"
     CDS             complement(30368..31312)
                     /locus_tag="ACL719_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS0698089.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            31444..34275
                     /gene="uvrA"
                     /locus_tag="ACL719_000034"
     CDS             31444..34275
                     /gene="uvrA"
                     /locus_tag="ACL719_000034"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGS0698090.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            34268..35329
                     /locus_tag="ACL719_000035"
     CDS             34268..35329
                     /locus_tag="ACL719_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS0698091.1"
                     /translation="MNKRVQAFLVKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            35461..35859
                     /gene="spx"
                     /locus_tag="ACL719_000036"
     CDS             35461..35859
                     /gene="spx"
                     /locus_tag="ACL719_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGS0698092.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            35925..36494
                     /locus_tag="ACL719_000037"
     CDS             35925..36494
                     /locus_tag="ACL719_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGS0698093.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            36581..36847
                     /locus_tag="ACL719_000038"
     CDS             36581..36847
                     /locus_tag="ACL719_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGS0698094.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            36851..37270
                     /gene="ruvX"
                     /locus_tag="ACL719_000039"
     CDS             36851..37270
                     /gene="ruvX"
                     /locus_tag="ACL719_000039"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGS0698095.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            37286..37591
                     /locus_tag="ACL719_000040"
     CDS             37286..37591
                     /locus_tag="ACL719_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGS0698096.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            38051..39301
                     /locus_tag="ACL719_000041"
     CDS             38051..39301
                     /locus_tag="ACL719_000041"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS0698097.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            39385..39843
                     /locus_tag="ACL719_000042"
     CDS             39385..39843
                     /locus_tag="ACL719_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGS0698098.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPVAQQQVQQPAQQ
                     NTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESD
                     GDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            39989..41521
                     /gene="cls"
                     /locus_tag="ACL719_000043"
     CDS             39989..41521
                     /gene="cls"
                     /locus_tag="ACL719_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGS0698099.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            41599..41742
                     /locus_tag="ACL719_000044"
     CDS             41599..41742
                     /locus_tag="ACL719_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698100.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            41779..43317
                     /locus_tag="ACL719_000045"
     CDS             41779..43317
                     /locus_tag="ACL719_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGS0698101.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMDVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            43437..45644
                     /gene="nrdD"
                     /locus_tag="ACL719_000046"
     CDS             43437..45644
                     /gene="nrdD"
                     /locus_tag="ACL719_000046"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGS0698102.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKGENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            45656..45796
                     /locus_tag="ACL719_000047"
     CDS             45656..45796
                     /locus_tag="ACL719_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698103.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            45840..46346
                     /locus_tag="ACL719_000048"
     CDS             45840..46346
                     /locus_tag="ACL719_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698104.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGLWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVKR
                     YWIEVANE"
     gene            46339..46929
                     /gene="nrdG"
                     /locus_tag="ACL719_000049"
     CDS             46339..46929
                     /gene="nrdG"
                     /locus_tag="ACL719_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGS0698105.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            46926..47543
                     /locus_tag="ACL719_000050"
     CDS             46926..47543
                     /locus_tag="ACL719_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS0698106.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            47810..48118
                     /gene="rpsJ"
                     /locus_tag="ACL719_000051"
     CDS             47810..48118
                     /gene="rpsJ"
                     /locus_tag="ACL719_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGS0698107.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            48334..48960
                     /gene="rplC"
                     /locus_tag="ACL719_000052"
     CDS             48334..48960
                     /gene="rplC"
                     /locus_tag="ACL719_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGS0698108.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            48985..49608
                     /gene="rplD"
                     /locus_tag="ACL719_000053"
     CDS             48985..49608
                     /gene="rplD"
                     /locus_tag="ACL719_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGS0698109.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            49608..49904
                     /locus_tag="ACL719_000054"
     CDS             49608..49904
                     /locus_tag="ACL719_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGS0698110.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            49922..50755
                     /gene="rplB"
                     /locus_tag="ACL719_000055"
     CDS             49922..50755
                     /gene="rplB"
                     /locus_tag="ACL719_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGS0698111.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            50859..51140
                     /gene="rpsS"
                     /locus_tag="ACL719_000056"
     CDS             50859..51140
                     /gene="rpsS"
                     /locus_tag="ACL719_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGS0698112.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            51152..51496
                     /gene="rplV"
                     /locus_tag="ACL719_000057"
     CDS             51152..51496
                     /gene="rplV"
                     /locus_tag="ACL719_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGS0698113.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            51509..52162
                     /gene="rpsC"
                     /locus_tag="ACL719_000058"
     CDS             51509..52162
                     /gene="rpsC"
                     /locus_tag="ACL719_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGS0698114.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            52166..52579
                     /gene="rplP"
                     /locus_tag="ACL719_000059"
     CDS             52166..52579
                     /gene="rplP"
                     /locus_tag="ACL719_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGS0698115.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            52589..52795
                     /gene="rpmC"
                     /locus_tag="ACL719_000060"
     CDS             52589..52795
                     /gene="rpmC"
                     /locus_tag="ACL719_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGS0698116.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            52820..53080
                     /gene="rpsQ"
                     /locus_tag="ACL719_000061"
     CDS             52820..53080
                     /gene="rpsQ"
                     /locus_tag="ACL719_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGS0698117.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            53106..53474
                     /gene="rplN"
                     /locus_tag="ACL719_000062"
     CDS             53106..53474
                     /gene="rplN"
                     /locus_tag="ACL719_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGS0698118.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            53552..53857
                     /gene="rplX"
                     /locus_tag="ACL719_000063"
     CDS             53552..53857
                     /gene="rplX"
                     /locus_tag="ACL719_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGS0698119.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            53881..54423
                     /gene="rplE"
                     /locus_tag="ACL719_000064"
     CDS             53881..54423
                     /gene="rplE"
                     /locus_tag="ACL719_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGS0698120.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            54441..54710
                     /gene="rpsN"
                     /locus_tag="ACL719_000065"
     CDS             54441..54710
                     /gene="rpsN"
                     /locus_tag="ACL719_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGS0698121.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            54995..55393
                     /gene="rpsH"
                     /locus_tag="ACL719_000066"
     CDS             54995..55393
                     /gene="rpsH"
                     /locus_tag="ACL719_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGS0698122.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            55585..56121
                     /gene="rplF"
                     /locus_tag="ACL719_000067"
     CDS             55585..56121
                     /gene="rplF"
                     /locus_tag="ACL719_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGS0698123.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            56205..56561
                     /gene="rplR"
                     /locus_tag="ACL719_000068"
     CDS             56205..56561
                     /gene="rplR"
                     /locus_tag="ACL719_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGS0698124.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            56579..57073
                     /gene="rpsE"
                     /locus_tag="ACL719_000069"
     CDS             56579..57073
                     /gene="rpsE"
                     /locus_tag="ACL719_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGS0698125.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            57087..57269
                     /gene="rpmD"
                     /locus_tag="ACL719_000070"
     CDS             57087..57269
                     /gene="rpmD"
                     /locus_tag="ACL719_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGS0698126.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            57414..57854
                     /gene="rplO"
                     /locus_tag="ACL719_000071"
     CDS             57414..57854
                     /gene="rplO"
                     /locus_tag="ACL719_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGS0698127.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            57867..59177
                     /gene="secY"
                     /locus_tag="ACL719_000072"
     CDS             57867..59177
                     /gene="secY"
                     /locus_tag="ACL719_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGS0698128.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            59328..59966
                     /locus_tag="ACL719_000073"
     CDS             59328..59966
                     /locus_tag="ACL719_000073"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGS0698129.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            60083..60301
                     /gene="infA"
                     /locus_tag="ACL719_000074"
     CDS             60083..60301
                     /gene="infA"
                     /locus_tag="ACL719_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGS0698130.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            60326..60442
                     /gene="rpmJ"
                     /locus_tag="ACL719_000075"
     CDS             60326..60442
                     /gene="rpmJ"
                     /locus_tag="ACL719_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGS0698131.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            60460..60825
                     /gene="rpsM"
                     /locus_tag="ACL719_000076"
     CDS             60460..60825
                     /gene="rpsM"
                     /locus_tag="ACL719_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGS0698132.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            60843..61226
                     /gene="rpsK"
                     /locus_tag="ACL719_000077"
     CDS             60843..61226
                     /gene="rpsK"
                     /locus_tag="ACL719_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGS0698133.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            61269..62204
                     /locus_tag="ACL719_000078"
     CDS             61269..62204
                     /locus_tag="ACL719_000078"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGS0698134.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            62216..62602
                     /gene="rplQ"
                     /locus_tag="ACL719_000079"
     CDS             62216..62602
                     /gene="rplQ"
                     /locus_tag="ACL719_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGS0698135.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            62867..63133
                     /locus_tag="ACL719_000080"
     CDS             62867..63133
                     /locus_tag="ACL719_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGS0698136.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            63143..64480
                     /locus_tag="ACL719_000081"
     CDS             63143..64480
                     /locus_tag="ACL719_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGS0698137.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     IGDSVARVLEEMGLETVGTHGTTVALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            64724..65416
                     /locus_tag="ACL719_000082"
     CDS             64724..65416
                     /locus_tag="ACL719_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0698138.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGCEKMEEGSI"
     gene            complement(65519..67164)
                     /locus_tag="ACL719_000083"
                     /pseudo
     CDS             complement(65519..67164)
                     /locus_tag="ACL719_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(67154..68245)
                     /locus_tag="ACL719_000084"
                     /pseudo
     CDS             complement(67154..68245)
                     /locus_tag="ACL719_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(68258..69379)
                     /locus_tag="ACL719_000085"
                     /pseudo
     CDS             complement(68258..69379)
                     /locus_tag="ACL719_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(69966..70742)
                     /locus_tag="ACL719_000086"
     CDS             complement(69966..70742)
                     /locus_tag="ACL719_000086"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGS0698139.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            70864..71610
                     /locus_tag="ACL719_000087"
     CDS             70864..71610
                     /locus_tag="ACL719_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0698140.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            71623..72603
                     /locus_tag="ACL719_000088"
     CDS             71623..72603
                     /locus_tag="ACL719_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGS0698141.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            72798..73118
                     /locus_tag="ACL719_000089"
     CDS             72798..73118
                     /locus_tag="ACL719_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0698142.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            73164..73472
                     /locus_tag="ACL719_000090"
     CDS             73164..73472
                     /locus_tag="ACL719_000090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0698143.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            73489..74787
                     /locus_tag="ACL719_000091"
     CDS             73489..74787
                     /locus_tag="ACL719_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0698144.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            74938..77376
                     /locus_tag="ACL719_000092"
     CDS             74938..77376
                     /locus_tag="ACL719_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGS0698145.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            77395..78063
                     /locus_tag="ACL719_000093"
     CDS             77395..78063
                     /locus_tag="ACL719_000093"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGS0698146.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            78081..79169
                     /locus_tag="ACL719_000094"
     CDS             78081..79169
                     /locus_tag="ACL719_000094"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGS0698147.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            79624..82125
                     /gene="leuS"
                     /locus_tag="ACL719_000095"
     CDS             79624..82125
                     /gene="leuS"
                     /locus_tag="ACL719_000095"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGS0698148.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDISREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            82425..83118
                     /locus_tag="ACL719_000096"
                     /pseudo
     CDS             82425..83118
                     /locus_tag="ACL719_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            83130..83546
                     /locus_tag="ACL719_000097"
                     /pseudo
     CDS             83130..83546
                     /locus_tag="ACL719_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(84361..84453)
                     /locus_tag="ACL719_000098"
     CDS             complement(84361..84453)
                     /locus_tag="ACL719_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS0698149.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            84587..85585
                     /gene="ruvB"
                     /locus_tag="ACL719_000099"
     CDS             84587..85585
                     /gene="ruvB"
                     /locus_tag="ACL719_000099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGS0698150.1"
                     /translation="MSRILDNEMMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            85566..86138
                     /locus_tag="ACL719_000100"
                     /pseudo
     CDS             85566..86138
                     /locus_tag="ACL719_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            86369..87127
                     /locus_tag="ACL719_000101"
     CDS             86369..87127
                     /locus_tag="ACL719_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGS0698151.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            87136..87939
                     /locus_tag="ACL719_000102"
     CDS             87136..87939
                     /locus_tag="ACL719_000102"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGS0698152.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            87961..89220
                     /gene="rseP"
                     /locus_tag="ACL719_000103"
     CDS             87961..89220
                     /gene="rseP"
                     /locus_tag="ACL719_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGS0698153.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            89233..91086
                     /locus_tag="ACL719_000104"
     CDS             89233..91086
                     /locus_tag="ACL719_000104"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGS0698154.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIVDRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            91186..92565
                     /gene="bglA"
                     /locus_tag="ACL719_000105"
     CDS             91186..92565
                     /gene="bglA"
                     /locus_tag="ACL719_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS0698155.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            92757..94565
                     /gene="glmS"
                     /locus_tag="ACL719_000106"
     CDS             92757..94565
                     /gene="glmS"
                     /locus_tag="ACL719_000106"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGS0698156.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            94836..95885
                     /locus_tag="ACL719_000107"
     CDS             94836..95885
                     /locus_tag="ACL719_000107"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGS0698157.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(95985..99827)
                     /gene="spuA"
                     /locus_tag="ACL719_000108"
     CDS             complement(95985..99827)
                     /gene="spuA"
                     /locus_tag="ACL719_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGS0698158.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            100244..100657
                     /gene="rpsL"
                     /locus_tag="ACL719_000109"
     CDS             100244..100657
                     /gene="rpsL"
                     /locus_tag="ACL719_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGS0698159.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            100677..101147
                     /gene="rpsG"
                     /locus_tag="ACL719_000110"
     CDS             100677..101147
                     /gene="rpsG"
                     /locus_tag="ACL719_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGS0698160.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            101572..103653
                     /gene="fusA"
                     /locus_tag="ACL719_000111"
     CDS             101572..103653
                     /gene="fusA"
                     /locus_tag="ACL719_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGS0698161.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            103757..108148
                     /locus_tag="ACL719_000112"
     CDS             103757..108148
                     /locus_tag="ACL719_000112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGS0698162.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            108250..108513
                     /locus_tag="ACL719_000113"
     CDS             108250..108513
                     /locus_tag="ACL719_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGS0698163.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            108506..108784
                     /locus_tag="ACL719_000114"
     CDS             108506..108784
                     /locus_tag="ACL719_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGS0698164.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            108805..108957
                     /locus_tag="ACL719_000115"
     CDS             108805..108957
                     /locus_tag="ACL719_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698165.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            108979..110220
                     /locus_tag="ACL719_000116"
     CDS             108979..110220
                     /locus_tag="ACL719_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGS0698166.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            110230..110460
                     /locus_tag="ACL719_000117"
     CDS             110230..110460
                     /locus_tag="ACL719_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS0698167.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT        32895 a        20259 c        24498 g        32881 t
ORIGIN      
        1 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
       61 tcgaagattc tgagggtaaa acctaatgga aagattattt gaaagtcaac agttccgatc
      121 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
      181 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
      241 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
      301 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattcagctt
      361 ttttctaaca cattcttagc tctccgtgtt gcttttttta atgaactaga tttttttgct
      421 gagaggagaa gtttaaatgc tgaagttgta ataaaaggtg tttgtttaga tccaagaatt
      481 ggaaattttt ataataatcc ttcttttgga tttggaggat attgtcttcc caaagatact
      541 aaacaattaa aaaaagaatt tatagaaata aatgccccag tgatagaagc gattgatatt
      601 tcaaacacaa atagaaaaca gttcatagtt aaacaaatat tggaacgaaa gccaaagata
      661 gtaggaatat ataaattggg gatgaaatat aattcagata attacaaaga gtcagctatt
      721 ttaagtataa ttaatgaact attgattgtc ggtataaaaa ttttagtata tgaaccaaat
      781 ttaaacgtta gtatagataa cgtaattttt gaaaagaatt ttgaactatt tacaaaacaa
      841 agtgatctaa ttgttgcaaa tagatgggat aggggccttg aagcttataa agataaagtt
      901 tacacaaggg ggatctggat aagagattaa ctattgatat taaaaaggca ataatttata
      961 aaatttgtac aaaaaaatgt taaattttca cattttcata gtatactgtt taatagaaag
     1021 taggttcttt gtaaccgctc cattataaaa tcctttcact cttccggtat actattagga
     1081 aaaacataac tggaggatat tttgagctca cacctcatcg ttccagtggc tgttccccaa
     1141 gctcgtgtac tgtccaaaaa agttagacat tctatttaga acgaggattg agttctgtat
     1201 tgtacaaggc tcggtccttt tagagtcagc ttaaggctgg ctttttcaat caccaaagtg
     1261 tcagaatgtt ttgacaaaac tataaaaaca atgatatact atataaaaaa taggaggtgg
     1321 tgagatgatc gagagaatgg aattgggaga attttacaaa gaactgcgcc tggctagaaa
     1381 gctcaagcag tcagatgtga cttgtgctgg tctaacaacc tctcagttgt ccaagtttga
     1441 actagggcag tctatgttgt ctgcagataa gctcatatta gccatccaag ggatcaatat
     1501 gaattttgat gagtttgggc acaagctcaa caactatcaa gaatctccac atatgcgatt
     1561 tggtagaaag gttgtggatc gctttgctca tcaagatata gctggcttag agcaactgtt
     1621 ggaggaagtc gagcaagaac agatggcaga gacttatcgt cgtttgaatg ctattgtaat
     1681 caaagatgcc atccactcac tagataaaag ctacccacta gcagaggagg atagcgagtt
     1741 tttgaccacc tatctctatg ctatcgagtc ttggacctgg tttgaactct atctcttttg
     1801 caatactatg cccttcttga gcaatcaaga tctgatcttt ttatcaacct ccttactcga
     1861 aaaatccaaa gaatttaaag agttagtaca caatcgattg tatatgaagc aaggactctt
     1921 aaatatcatc tcagagctca tggagcgcaa acttttttct tatatcccaa tctttgaagc
     1981 cgagctggag agtatgctcc gtccatacga tgtttttgag aaattattgt ggcagttttt
     2041 aaagaagatg agcattttcc ttcaaactaa gggaagcaat caaaaagaga ttgaacactt
     2101 tatccaatct ctgcaagtat tagaaaatcc acaattaata gccctttttg aattgcgttt
     2161 gcagcaatac aaaaagctta tcgattagtt agaaatggca aaataataat aatctcagca
     2221 acgattttca ataatgttaa aatttgtttc ttcatatttc tccactcctc attagtagta
     2281 actttattat accagagaag taatcaaatt caccaaaatc gcaaatctga aatttctata
     2341 agaaaaaata tcaaatatgc gattttctaa aataagccaa ttttcgtgtt atactgtact
     2401 tgtaaagcac ttgaagcaaa tcctaggtcg cagaagtggt ttacaaatga agataattga
     2461 aggagtgtaa aagatgttaa atttacaatt tgcagaaaca atggaattga cagaagctga
     2521 gttgcaagat gttagaggag gcaacctcgt aaattctatg ggaggtggtg gaagaagtgg
     2581 tatatctggt tggggcgtcc caggaattta ccctggttgg ggcaatcaag ggatgagtcc
     2641 taatcgtgga gcttttgatt ggactataga tttagcagat ggattgtttg gccgccgtag
     2701 aagataggct cttataggag ataataggat gaagaaaatg aaagaagtga aatttcacct
     2761 agcaactggc ttgttgattt tgacttatta cctcattttt aatgtcacat cagacttaga
     2821 ttttatggtt gctttgtctg ataatatgta ctatgtgttc caagttttat tggtacttat
     2881 tctgggaact atagctacaa ttgcttttgt caaaagtgaa cattggaaag agtgtggtcg
     2941 tttccagttt cgttggtttt acctgggtgt tttccttctt tcttttttct tattgttcgt
     3001 gtgggcaaat ctaacaacct atatctttcc tcgtacccag aatggaagta cagtcgtaga
     3061 agtagccacc aatttaacag gaattagcta ttttgttacg cgaatacttt atactagtat
     3121 tatcgcacct gtatcagaag aggttgtttg tcgtggtctt ttgatgacaa gtttatctaa
     3181 agttaagaga tactatcttg atgttcttgt ttccgctgcc atatttggcg ctatgcatgt
     3241 tttgcagtat ggttggataa cgacagattt cataaagtat tttggaatgg gcttgatatt
     3301 ttgtatgatg tttcgctaca cccgatccat ctattgggct atagctctgc atgcttcatg
     3361 gaatagtttc ttactcattg ttactttatt ggtatttgga tattagaaag gtgatatatg
     3421 aaaaagataa tctcacaccg ctactttatt atagtttttc tactagttat tgctgaccag
     3481 aagttcagtg ttctagtttt acgcagcgac cttgttactg ggctatctga ctttgcctat
     3541 tatctgtcgg atatgatgtt gaattttctt gtggttttat ttgctcttat tgctatgatt
     3601 tggtcgggga aatggcagaa aatcaatagt agaaagttta aaggatccta tcttttctat
     3661 tccttcctag ctctgcttgc ttttgttgtt tggaatttcg ttaccttttt tcttttccca
     3721 cctactcgaa atgaaatttc ttatcaacat gctgctccga cttttacagg ggctacagca
     3781 tttttgatgt attttttcta tcctgtgatt gcaggtccca tttttgaaga gataatccat
     3841 cggggattgg tgatgacagt tctggaaaaa ggaaagaaat gggggctaga tgtacttggt
     3901 tccgctgttt tatttggggt ctcgcacatt agtaatcacg gttgggtctt gacagacttt
     3961 gtcttctata tgggtggagg tcttatattt gcagtcttat ttagaatgac aaagtcaatt
     4021 tattggccta ttggactgca tatagtctac aatggcattg gtcagctttt gatgttactg
     4081 taattttggt tgttagtagt atcgcggtct atccttgggg aaagcttccc atttgaactt
     4141 aagaaggagt tgtgatgaaa gtatttcttc aaaatagaga ttttaggcaa ttaactatca
     4201 accagtggat ttcaacgctt ggggatacga ttttttatct ggccttcctg aattatgtgg
     4261 cagatgcatc ttttgcccct ttggcgattt tactcatcac gatttcagaa accctgcccc
     4321 aagttctaca aatctttctg ggagttttgg cggattttca acatcatcgt gtcttgaaat
     4381 acacagtcat tagttttgca aaatttttgc tttactctat agtgtcttta tcactttcag
     4441 ggcagtcctt ttccttgtta ttagtagcat ttatttgtct gattaacctt ttgtctgaca
     4501 cgttgagtta tttttcaggc gccatgctca ctccgatttt cattagaatt attgggcaag
     4561 accatctggc agaagctatt ggatttaaac agtcaactgt tagtttagtg aaaacaatca
     4621 gtaatattct aggaggagtc ttattaggta ttctatccat ccagtttatt tccttgctga
     4681 acgctcttac ctttttaatc gcatttttag gtatcctctt cataaaaact gacctcttga
     4741 aagtagaaaa aacgattaac tatcaagaag gactctctgt aaaatccttt tgccagcatt
     4801 tgctccaatc atcgaaattg atatggaata tgaataaggt gctcttggtt ttgtttatta
     4861 tctctactag tcaagcagtg ataaatgtta cagttcctgt ctctactcta tttttgagga
     4921 atcaaccctt tttgaattta caaacaggtc aatctcttgc cttgctatcc acttttgaac
     4981 tgtcagccct gattgtcgga agccttgtaa gtggctatct gcaacatacc atttccataa
     5041 aaacagctct ctatgcctca cttgtcatcc agttgcttct tctagtagga tttgccacag
     5101 ttcgttttga ctggattctc atctttagta ccttggatgc ctttttcgca ggtgttctct
     5161 ctcctagatt gcaagaactc gtctttaaac aaatacctga ggagtcaatg ggagcagttc
     5221 aatcttctat cagtgccatt acggttgttt tacctagctt atttacaata tctttggtaa
     5281 ccattgctac tagctttgga actctggcag ttagctttgt tttattgcta tttcttctag
     5341 ttgcctttgt tatgcttctg aatattcgtg aaagtattta gtggagaatt tctataatat
     5401 atgtttgaga ctggattgcc agtcttttta tgatggtcaa aatcaacaga cttttttctc
     5461 ttttctgaat gtgttatagt agactgaatc taaaatagta cgaaacaatt gctaaaacat
     5521 ttatagaaat taattttact ttcccaatcg atttgttcat atcttatttc aatccattat
     5581 attaagacaa tatgaggaga aagatgaaag aaaaaggatt ttaggagggg gtgcaggcag
     5641 tcatgccaac ggctcttggt tatgtcagta tcggcctggc ctgtggaatt atcggtgcgc
     5701 cctatgtgac acctgttgag atgggcttga tgagtctctt tgtttatgct gggagtgccc
     5761 agtttgccat gttggcactg attgtggttc aagctcctgt ggcagctatt gctatgacgg
     5821 tttttctaat caacttgcgt ctctttttgt tgagtttaca cgcatcgact tatttccgtc
     5881 ataccagtct ttggtacaat atcggtatgt ctagtatctt gacagatgag acctatggcg
     5941 ttttgatggg tgaattggcc catacagaca aggtaaatcc tatgtggatg cacggaaaca
     6001 atcttaacag ctatgtggct tggtttgtgg ggacagtagt cggaacggct ctgggtggcc
     6061 tgctaccaaa tccagaaatc tttggcttgg attttgccct ggttgggatg tttattggta
     6121 tttttgcttc gcaatttcag attatgcaaa gacggattcc tgtccgcaat ctgctcatta
     6181 tcctagcagt tgttgcggtg tccttctttt tgctcttgac agtggtgtct cagtcactag
     6241 ctgttctgtt tgcgacgcta cttggttgta gcatgggggt ggttttagat ggtcagtaag
     6301 tatcttttat tagcagttat tttctctggc ttggtgactt ggattctccg tatgattccc
     6361 ttcatcttag tcaagtataa gggcttgcct gcaatcgttg agcgtttttt gaagttcttg
     6421 cccgtttcca ttatctttgc cttgattctt tcaagcgtag tgacaggtaa ggttgggagc
     6481 cttcctcaaa ttaaatggct agacttctta gccgtctttc caacagcttg ggtagccttt
     6541 cgctaccgca atctagtcgg tacggttctc tttggagtag tcttgattgc cgtcttgcgt
     6601 ttggtctttt aaataagtca taaagaaaac ttatcacaga gatagatatc atataatggc
     6661 gtaattactt tttttctgat aagattataa ggtattctat tttggaggaa atgacatgaa
     6721 aaaaatcgtt aaatactcat ctcttgcagc ccttgctctt gttgctgcag gtgtgcttgc
     6781 ggcttgctca ggtggtgcta agaaagaagg acaagcagct agcaagaaag aaatcatcgt
     6841 tgcaaccaat ggatcaccaa agccatttat ctatgaagaa aatggcgaat tgactggtta
     6901 tgagattgaa gtcgttcgcg ctatctttaa agattctgac aaatatgatg tcaagtttga
     6961 aaagacagaa tggtcaggtg tctttgctgg tcttgacgct gaccgttaca atatggctgt
     7021 caacaatctt agctacacta aagaacgtgc ggagaaatac ctctatgccg caccaattgc
     7081 ccaaaatcct aatgtccttg tcgtgaagaa agatgactct agtatcaagt ctctcgatga
     7141 tatcggtgga aaatcgacgg aagtcgttca agccactaca tcagctaagc agttagaagc
     7201 atacaatgct gaacacacgg acaacccaac tatccttaac tatactaagg cagacttcca
     7261 acaaatcatg gtacgtttga gcgatggaca atttgactac aagatttttg ataaaatcgg
     7321 tgttgaaaca gtgatcaaga accaaggttt ggacaacttg aaagttatcg aacttccaag
     7381 cgaccaacaa ccgtacgttt acccacttct tgctcagggt caagatgagt tgaaatcatt
     7441 tgtagacaaa cgcattaaag aactctacaa agacggaacg cttgaaaaat tgtctaaaca
     7501 attcttcgga gatacttatc taccagcaga ggctgatatt aaataatttc ttgaaatcat
     7561 ccattctgag taagatgggt gatttctcag tttttaggga tatagtttta aactatatat
     7621 ctgattatct aataacaatt tgtgaaatca aacaaattgt gagatactag tacggtatta
     7681 tttttaagga gaaagaatca tgaaaatcaa aaaatggctt ggcctagcag cccttgctac
     7741 agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa aagaaagcag acaatgcaac
     7801 aactatcaaa atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat
     7861 ccaagaattg gttaaaaaag acggaattac cttggaattt acagagttca cagactattc
     7921 acaaccaaac aaagcaactg ctgatggcga agtagatttg aacgctttcc aacactataa
     7981 cttcttgaac aactggaaca aagaaaacgg aaaagacctt gtagcgattg gagatactta
     8041 catctctcca atccgccttt actcaggttt gaatggaagt gccaacaagt acactaaagt
     8101 agaagacatc ccagcaaacg gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg
     8161 tgcgctttac ttgcttcaat cagctggctt gattaaattg gatgtttctg gaactgctct
     8221 tgcaacagtt gccaacatca aagaaaatcc aaagaacttg aaaatcactg aattggacgc
     8281 tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt
     8341 tacagaagca aaattggact acaagaaagc acttttcaaa gaacaagctg atgaaaactc
     8401 aaaacaatgg tacaacatca ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga
     8461 tgctatcaag aaagtaatcg cagcttacca cacagatgac gtgaaaaaag ttatcgaaga
     8521 atcatcagac ggtttggatc aaccagtttg gtaataagaa acagggaggt gggagagaga
     8581 attccacctc ttgcttttgt atagagcacg gagtgtaaag aagactattc gttcatagaa
     8641 aggtagagag aatatggttt ttcctagcga acaagaacag attgaaaaat ttgaaaagga
     8701 tcatgtagcc cagcattatt ttgaggtttt acgtaccttg atttctaaga aatcagtctt
     8761 tgcccagcag gttggactca aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt
     8821 tggagctgaa gtggagattg atgagagcta tacagcgccc tttgtcatgg cacatttcaa
     8881 gagttcgcgt ccagatgcca agaccttgat tttctataac cactatgaca ctgtgccagc
     8941 ggatggggat caggtctgga cagaggatcc ttttacgctt tcggtccgca atggcttcat
     9001 gtatgggcgt ggggttgatg acgacaaggg tcatatcaca gctcgcttga gtgctttgag
     9061 aaaatatatg cagcaccatg atgatttacc tgtcaatatc agctttatca tggagggagc
     9121 ggaggaatcg gcttcaacag acctagataa gtatttggaa aagcatgcag acaaactccg
     9181 tggggcggat ttgttggtct gggaacaagg gaccaaaaat gccttggaac agctggaaat
     9241 ttctggtggc aataagggga ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga
     9301 tatccactcg agttatggtg gtgttgtgga atcagctcct tggtatctcc tccaagcctt
     9361 acagtctctt cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg aagaagtaca
     9421 agagcccaat gaacgagaaa tggccttgct agaaacttat ggtcaacgaa acccagagga
     9481 agttagtcgg atttatggat tggagttgcc tctcttacag gaggagcgga tggcctttct
     9541 aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga atccagtctg gttatcaagg
     9601 tcagggtgtt aagactattt tacctgcaga agccagtgcc aagctagagg ttcgtctggt
     9661 tccgggtcta gaaccgcatg atgttctgga aaaaattcgg aaacaactag acaaaaatgg
     9721 ctttgataag gtagaattat actatacctt gggagagatg agctatcgaa gcgatatgag
     9781 cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa ttctatccac agggcgtttc
     9841 agtcttgccg acgacagcgg ggacaggacc tatgcatacg gtctttgatg ccctagaggt
     9901 atcaatggtt gcattcggtc taggaaatgc caatagccga gaccacggtg gagatgaaaa
     9961 tgtgcgaatc gctgattatt acacccatat cgaattagta gaggagctga ttagaagcta
    10021 tgagtagaga tattatcaag ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa
    10081 ccattacagc ggttaaggat gtgaccattc acatccaaga aggggatatc tacggaatcg
    10141 ttggatattc tggagcagga aaatcaaccc ttgtacgggt gattaacctc ttgcaaaaac
    10201 catctgcagg gaaaattacc attgacgacg atgtgatttt tgatggtaag gtcaccttga
    10261 cggcagagca gttgcgtcgt aaacgtcaag atatcgggat gattttccag cattttaacc
    10321 tgatgagcca aaagacagca gaggagaatg tagcctttgc ccttaaacac tctggactca
    10381 gcaaggaaga aaagaaggct aaagtagcta agttgttgga cttggttggt ttggcagatc
    10441 gtgctgaaaa ctacccttca caactatctg gagggcaaaa acagcgtgtg gcaattgcgc
    10501 gtgccttggc caatgatcca aaaatcttga tttcagacga gtcaacttct gcccttgacc
    10561 ctaagacaac caagcagatt ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga
    10621 ctgttgtctt gattacgcat gaaatgcaga ttgtcaaaga cattgccaac cgtgtggcgg
    10681 ttatgcagga tgggcatttg attgaagagg gtagtgtgct tgaaatcttc tcaaacccta
    10741 aacaaccttt gactcaagac tttatctcaa cagctacagg tattgacgaa gccatggtca
    10801 aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg gtgcaactca
    10861 agtacgctgg agcttcaaca gacgagccac ttttgaatga attgtacaag cattaccaag
    10921 taatggctaa tattctctat gggaatatcg aaattctcga tggtactcct gttggagaat
    10981 tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg tgcccaagaa gctattcgcc
    11041 aagcaggtgt acaactaaaa gtattgaagg gaggacagta agatggaatc attgattcaa
    11101 acctatttac caaatgtcta taaaatgggt tgggctggtc aggcaggctg gggaacggct
    11161 atctacttaa ctctttatat gacagttctt tccttcatta tcggaggctt cttggggcta
    11221 gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg tcttggagaa taaagtcgta
    11281 ttctggattt tagacaaaat tacctcaatt tttcgtgcgg ttccctttat catcctcttg
    11341 gcaatcttgt caccactttc tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc
    11401 cttgtcccac tttcttttgc agtttttgcc ttctttgccc gtcaggtgca ggttgtcttg
    11461 gctgaattgg atggcggtgt cattgaggcg gctcaagcga gcggagcgac tttctgggac
    11521 atcgtgggtg tttacctatc agaaggtctt ccagatttga tccgtgtgac gactgtgacc
    11581 ttgatttccc ttgttgggga aacagctatg gccggtgcgg ttggagctgg tggtatcggt
    11641 aacgtagcca tcgcttatgg atttaaccgc tacaatcacg atgtgaccat cttggcaacc
    11701 atcgttatca ttttgattat ctttgcaatc caattcttag gagatttctt gactaagaaa
    11761 ttgagccata aataaaaaag agccgtgtgg ctctttttaa ctgatcagat tttctgggca
    11821 aattttttac tcaaggcttg tccaatcaag gcacccacta gggctccgat gacaatactt
    11881 gcgataaata gaaggacagt tccaggggtt ggagtgacca tgatacggtc gatatattct
    11941 tgggattttc ctcttgccag aagagtagcc atataggctt tgggcgcaat ccacataagc
    12001 aagattggtc ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg
    12061 tccttgtatt ttcctaaatg agctactcca tctgctagga ggccacagat aattccagga
    12121 aggaaggaac cagcaccgtg tttagttccc aagaaaaaga gggcaatgac aaggccgata
    12181 gtggtaatgg ctccaaagcg cggaactttt gcgactagga tcatatagac gctaccgccg
    12241 acaagggcag taaaggcagg cgcataaaac atgtttccag tttggtcaaa gagattgccc
    12301 aaaaggacac caatccccat gcagaggaag taaagaactg caaaaagcag tgtagttaag
    12361 atagatttct tcatgaattg tctcctgata attttttcac aattctcatt ttatcatggt
    12421 ttggtgggat tgtaaatgga tactagaagt ttttgaaaac gcttgaaata tgatataatg
    12481 gtttcatagt tatttttagg ggaatatcat gggtagattt ttagactttg tctttaatcg
    12541 tttcttttta gggatgattg cgacagcctt attttggcta ttaactttag caggagggat
    12601 tatccttggt ctagcgccgg ctagtgccac cttgatgagc ttatatgcag aacatggtta
    12661 tagctttcgg gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa attttgtctc
    12721 aagcaacctg attttctata gctttttagg tgtgggtcta gttttgacct atggtttgta
    12781 tctcttggtg caattgcctc atcagaccat tgttcatttg attgcgaccc ttttgaatgt
    12841 cctagtagtt gccctgatct ttttggctta tacagtatct ttaaaattac aagtttattt
    12901 tgccttgtcc tatcgaaata gtctcaaatt atccttgatt ggcatcttta tgagtctagc
    12961 agctgtggct aaggttctcc ttgggactgt gctacttgta gcaattggtt attatatgcc
    13021 tgccctgcta ttttttgtag gaattgggat gtggcatttc tttatcagtg atatgttgga
    13081 acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag cagttttggc
    13141 tacatacgct tctaagaacc tatagttcag tgatgatcat tatcattgcg agttttgcaa
    13201 tcttactctc ttacgctgac tgggattcac gtgaaaagga agcccagaga gtagcccagc
    13261 gtgtaactgc tagaacagtg agtgaaattg aatattacca tagagagtca acccagatag
    13321 ctcaggcttt agttgaaaat caagctcgta tcgagggaat ctataaatac tttagcctta
    13381 gcatgccaga ctatttttac tggcaattag agcggaaagc ttcgccttat atatcagtct
    13441 ctctgtatga aaatgttgat gacctctatg ttcgaaatga ttttgtaact ggggtggcca
    13501 ttgcttttca agattacaag gaagtctatg tttctactaa agacaaacgt agtggagaaa
    13561 aaatcagggc tgaggatttc aaaccagcag gaaatagttt tgccattccg gtgtcagatc
    13621 cagtgtcaga tcaagattta ggagtgattt acatctcctt ggatcctgct gttttatacc
    13681 atgccattga taatactaga ggtcatactc cgatggcagt aacagtgacc tcaccttttg
    13741 atacggagat ttttcatatt ggtgagacag ttgataagga gagtgaaaat tggctagttg
    13801 gcttaacttc tcatggatat caggttcagg tggcagttcc taaaaacttt gttttacaag
    13861 gaacagtgac tagctctgct ttgattgtgg gtttgagcct tctctttatt gtcattcttt
    13921 atctgacttt gaggcagact tttgctaatt accaaaagca ggtagtggat ttagtagaat
    13981 ccattcaagt cattgctcaa ggcgaagagg ggcgtcggat tgacatttcc gagaaagatc
    14041 aggaattgct cctaatcgcg gagacgacca atgatatgtt ggatcgattg gaaaagaata
    14101 tccatgatat ttaccagtta gaactcagtc aaaaagatgc caatatgcgg gccttgcagg
    14161 cgcaaatcaa ccctcacttc atgtataata cgctggagtt cttgcgcatg tacgcagtca
    14221 tgcagagtca agatgagttg gcagatatca tttatgaatt cagtagtctc ttgcgtaaca
    14281 atatttccga cgaaagagag accctcctca aacaggaatt agaattttgc cgtaaataca
    14341 gctatctctg catggttcgc tatcccaagt ccattgccta tggtttcaag atagatccag
    14401 agttagagaa tatgaagatt cccaagttta ccttgcaacc gctggtagaa aattatttcg
    14461 cgcatggtgt tgaccacagg cggacagata atgtgattag catcaaggct cttaaacagg
    14521 atggttttgt ggaaattttg gtggtcgata atggtagagg aatgtcggct gaaaagttgg
    14581 caaatatccg agaaaaatta agtcagagat attttgaaca ccaagccagc tacagtgatc
    14641 aaaggcagtc tatcgggatt gtcaatgtac acgagcgttt tgtgctctat tttggagacc
    14701 gctatgccat tacgatagag tctgcagagc aagccggtgt tcagtatcgt attacaattc
    14761 aagatgagta gaaagggaga aaatgtataa agtattatta gtagatgatg agtacatggt
    14821 gacagaaggt ctgaagcgtt tgattccctt tgataagtgg gatatggagg tcgtcgcaac
    14881 agccagtcat gccgatgaag ctctagaata tgttcaggaa aatcctgtcg atgtcatcat
    14941 ttccgatgtc aatatgccag acaaaacagg gcttgatatg attcgggaga tgaaagagat
    15001 cttaccagat gctgcttata tcctgctctc aggttaccag gagtttgatt atgtaaaaag
    15061 agcaatgaac cttagtgtgg tggactattt ggtcaagcct gttgataagg tagagctggg
    15121 aaatctgctg gagaagattg caggtcagct cggcgagaga gggaagaaaa gtcagactct
    15181 tagtcaagaa ttagacgagg ctggatttgt tagttattta ggggataagg agaattggtg
    15241 gataggtcta tccaaggaaa aacaaggttc cttcaccatt ccctactatg tcttgggtca
    15301 agactggcag attttcattt ctgaccaacc cctagatggt ttagtcgtta caccttttga
    15361 agctccttat caagaacact ttgaacgctg gaagctgaat gctgagaaaa ccctctttta
    15421 cggctctgta aatctgcagc agtctgagag tctctttgcc tattacgaac cgatttatag
    15481 ggttatcatt cagggaaatc tcaatcaaat cgtagaagag ttaaatctct tggagaaggt
    15541 agttcttgaa aatacgccgc gtgttccgat tactaaacag ctttttattc agtttgtcat
    15601 ggatgttttc catttatttg aacatctcaa agctgatgac ctgacagaca tcgtcaaaac
    15661 cattcatgct attcaatcct tcgatgaatt ggttccttat atcaaggaaa ctctgagcag
    15721 ctttttcggt caataccgta tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg
    15781 tgattaccaa aaagagcttt ccctcaagga tatcagtaag gctctcttta tcaatcctgt
    15841 ctatctaggg cagttgatta agcgtgaaac cgattcgact tttgcagagt tactaaacaa
    15901 acaacgtatt aaggctgccc aacaactttt gctttcaact agtgacagca tcgaagatat
    15961 ttgttatgct gttggttaca gtaaccttgg atatttctat aaagttttcc gaaaattgtg
    16021 cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact atactataaa atttgtattc
    16081 ctttacaaaa ggtgctataa tattatcagg aggttctatc atgaaaaaga aaccaattta
    16141 tctatgggtc ttgctaatct tgtctgccct tatttcagtt ccgtctctgt ttggaattgt
    16201 gagtcccttg cctagtaaag aagcccttcg tgctgctcag aaacaagttg caggggtcaa
    16261 tgctcagcaa ttagaagacc agcttaatta tacctataga gtagcagaag catctcattc
    16321 tatttttaat gttgctttga ttgtgctatc tactatttta gtggtcgtag cgattgtctt
    16381 ccttgttcgt aaaaatttgc aatatgcaaa ctatacttac gttggctatg ttttgttagc
    16441 tattattggt tcgatttatg gctatgttgg tttacaagac gctgtgcagt tggttcaaga
    16501 tgagagcatg cgtttgacag tgagtattgg ttcaaaagct gttagcattt tctatatcgt
    16561 catcaatgtt ctcttcctag cccttgtctt ctataagatg tggcgacaac agaaagcctt
    16621 ggcagaggaa gaggaaacag aagagcttgc ctaagtattg acaaaaaaga actatcaaga
    16681 tacaatcttg gtagttgttt tttgattata gataaaatca tggaggtaca tatgaagaca
    16741 atttctttag tttatattag tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa
    16801 gactatctct tgtcccagta caaaaggatt gaggttcaaa agattcatat caaggatttg
    16861 gtcaaggaag gcaaaaattt ctatgaaatg gaccatccct atgtcgcttt tttgccgacc
    16921 tatctcgaag gtgggaatgg cgtggataac ggagatgttg agattttgac gacaccggtg
    16981 ggagatttta ttgcctatgg taacaatgct agtaagtgtt ttggtgtggt tgggtcagga
    17041 aatcgtaact ttaataacca atactgccta acagccaagc aatacagtca acgctttggc
    17101 ttccctgtat tggctgactt tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca
    17161 gctattatcg cagatttgta tgagttggaa aaggagaatt aatactcaat gaaaatcaaa
    17221 gagcaaacta ggaagctagt cgcaggttgc tcaaagcaca gctttgaggt tgcagataga
    17281 gctgacgtgg tttgaagaga ttctcgaata gtataaagtt cttataagag gtggttgaag
    17341 ttgttcaact gttttttgag tataaacagt ctttaagaac gtaaaaaagc ataagttcag
    17401 gtcttcaaaa ccttgttctt atgcttttta tcatagaaat atttgctgaa ttttctcttg
    17461 aaatgaaacc ttatcttcat ctcgcgcttg tcccttaccc tgctttttcg attgcgagtg
    17521 cgtagataaa gaagatggcg aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt
    17581 tgagccaaga aggaagattt ttgttttcac gcgttaccaa ggcgtaattc aaaagagcaa
    17641 agaaaggtgt tgtcaggaaa gatccaatca tggcaaagcg gagcatggtt gaaacctgac
    17701 cagcgaagaa cttgataatg acgataccga tgatagcagt gatggtcatc cagatgttca
    17761 aagatttacg attgtcctct ttttgactga ttagcagtcg gagagattcc tgattaacgc
    17821 gagaatagcc atcgataaca gttataactg ttccaaagat acagaggaag gcaataaagg
    17881 taatcaagta acgggaccat tcgccaagaa cagaggcata catgcccacg aattgagaga
    17941 tgtatttggc tgaagcagct tcaaccgcct gccctgtagg atactgaatc agtgctccca
    18001 gtgccacaaa gaagacggct aggatagctg ttccaatata accagtgtta aagtcaaaca
    18061 gagcgtcttc tgtgttaaag ttgacggtct ttctcttttc agctgaccaa agtgaattga
    18121 tggctgaaat ttcaatagga gccggcatcc atcctaggag ggagacgatg aagggcagag
    18181 ctgccatttg ccaaggtgtc ttctcgacaa aatcagaact gtattctgga tgcttgaccg
    18241 ctgctatgat aactgccaga acagtcgcaa tggttaaggc agacataatc catttgacca
    18301 tgccgtctaa aagtttgtag cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa
    18361 tgagggacca ctgagtaatg ctaagtccaa tcattgggaa ggcactggcg atgatagctg
    18421 agcacagaat ggcaacacca gccgtgttga ccatagccga aaagacattg aggataaaga
    18481 aaatccagag atagagtttt cctttttcgg cataaccttc aaccaaagtc tttccagtat
    18541 cagctgtgta ttcagcacca aaacggaaaa atggatattt aaagacattg gctaagatga
    18601 ccaagagaag tagagaccaa ccgtaagaac cgccagcttg agttgaggat acaatgtggg
    18661 aacctccaac agcggcagtt gccattagga ttccaggtcc cattgctttt agcttgcttg
    18721 cccaggttga ttgatttaag gaaatagctt gcgacatgat aaatactcct ttttgaattt
    18781 tcagaataat ctgtatatgt aatctatatt ctacaaaaaa ttttggaaaa atgcaagaat
    18841 attttgaaaa aagatagaaa atttacaaag aaaatctgaa ataactgtta tgatatagca
    18901 tattgaaact agaatagtac acctttgctt ctaaaacatt gttagaaatc gatttgactg
    18961 tcctgatcga tttatcctgt tcttatttca tttcactata gtaaggtgca gcaggataaa
    19021 gaaaaaccga gaagtttctt tctcggattt gcttattatg aggtttctat tttctatgct
    19081 ccaagagttg attgatgtga tctacttttt tagcctctct tttatagaga atagcccaga
    19141 tgatgagata gacaatcgca aactcgataa tgagctggag gtagaaaatc cagtggaaag
    19201 ggaaccaacc agcaagagtt gctagtggga caaagccagc cagcataagg aagaaatgag
    19261 tcagagtggc acgaagcatg ctccagtcac ggctgaataa gcggttgcca aagttgaaga
    19321 gcataccgat ggctgcccag ataagtgtgc agtagagcaa gaccagggca ccgtgaacct
    19381 gatgctgatc catcacttgg ccgatgagag agtagggatt tagtggtgcg taggtatttg
    19441 gtgcataaat gagtgaaaag aggatggaga ggatgaggcc gataagaaca ccggcagctg
    19501 catcgtgaaa tacttgtttt ttcatagttc taatttctcc ttgatggttt ttagataacg
    19561 gcgtgaagag taggtgaagc tttcgttttt taagaaaatt tctaccagac cgtttggcgt
    19621 gagcttgagg tgagagatgg aatcgatatt gatgatttct gattgggaaa tttggataaa
    19681 attgcttggc aagagtttaa gaacctgata gagtcgcaaa tcaatgctgt aggtctgact
    19741 cgcggtttct gctagaacct tccgattctc tatatagaag cgctgaatct tgccaatctc
    19801 aactagatag acctgatcat cgattttccc tttgattttt tctctttggt ccagattttc
    19861 tgcgaactcg atgactttct ggactttatc tgtcggctga ggtgcttgga caatcagctt
    19921 ttcctcctcg taagtctcac taatctgtag ttctactttc ataaatttct ctccttgttt
    19981 ttcatacaaa attaatctca cttcttgtac acctttatta atactcttca aaaatctctt
    20041 caaaccacgt cagcgtcgtc ttgccatagg atagttgttg actttgtcag ttttatctac
    20101 aacctcaaaa cagtgttttg aactgtctgc gcctagcttt ctagtttgct ctttgatttt
    20161 tattgagtat accactattt tactccctct ggcaagggac tttgtctatg tggagggatt
    20221 gggctcctat gtggtggagc ttttctgttc tttctgaaat atggtataat agcactaatt
    20281 aatttctagg aaaatagata cagaaagggg ctgaaagatg tctcatatta ttgaattgcc
    20341 agagatgctg gcaaaccaaa tcgcggctgg agaggtcatt gaacgtcctg ccagtgtggt
    20401 caaagagttg gtagaaaatg ccattgacgc gggctctagt cagattatca ttgagattga
    20461 ggaagctggt ctcaagaagg ttcaaatcac ggataacggt catggaattg cccacgatga
    20521 ggtggagttg gccctgcgtc gccatgcgac cagtaagata aaaaatcaag cagatctctt
    20581 tcggattcgg acgcttggtt ttcgtggtga agccttgcct tctattgcgt ctgttagtgt
    20641 cttgactctg ttaacggcgg tggatggtgc tagtcatgga accaagttag tcgcgcgcgg
    20701 gggtgaagtt gaggaagtca tcccagcgac tagtcctgtg ggaaccaagg tttgtgtgga
    20761 ggatctcttt ttcaacacgc ctgcccgtct caagtatatg aagagccagc aagcggagtt
    20821 gtctcatatc attgatattg tcaaccgtct gggcttggcc catcctgaga tttcttttag
    20881 cttgattagt gatggcaagg aaatgacgcg gacagcaggg actggtcaat tgcgccaagc
    20941 aatcgcaggg atttacggtt tggtcagtgc caagaagatg attgaaattg agaactctga
    21001 cctagatttc gaaatttcag gttttgtgtc cttgcctgag ttgactcggg ctaaccgcaa
    21061 ttatatcagc ctcttcatca atggtcgtta tatcaagaac ttcctgctca atcgtgctat
    21121 tttggatggt ttcggaagca agcttatggt tggacgtttt ccactggctg tcattcacat
    21181 ccatatcgac ccttatctag cggatgtcaa tgtgcatcca actaagcaag aggtgcggat
    21241 ttccaaggaa aaagaactga tgaccctggt ttcagaagct attgcaaata gtctcaagga
    21301 acaaaccttg attccagatg ccttggaaaa tcttgccaaa tcgaccgtgc gcaatcgtga
    21361 gaaggtggat caaactattc tcccactcaa agaaaatacg ctctactatg agaaaactga
    21421 gccgtcaaga cctagtcaaa ctgaagtagc tgattatcag gtagaattga ctgatgaagg
    21481 gcaggatttg accctgtttg ccaaggaaac cttggaccga ttgaccaagc cagcaaaact
    21541 gcattttgca gagagaaagc ctgctaacta cgaccagcta gaccatccag agttagatct
    21601 tgctagcatc gataaggctt atgacaaact ggagcgagaa gaagcatcca gcttcccaga
    21661 gttggagttt ttcggacaaa tgcacgggac ttatctcttt gcccaagggc gagatggact
    21721 ttacatcata gatcagcacg ctgctcagga acgggtcaag tacgaggagt accgtgaaag
    21781 cattggcaat gttgaccaga gccagcagca actcctagtg ccctatatct ttgaatttcc
    21841 tgcggatgat gccctgcgtc tcaaggaaag aatgcctctc ttagaggaag tgggcgtctt
    21901 tctagcagag tacggagaaa atcaatttat tctacgtgaa catcctattt ggatggcaga
    21961 agaagagatt gaatcaggca tctatgagat gtgcgacatg ctccttttga ccaaggaagt
    22021 ttctatcaag aaataccgag cagagctggc tatcatgatg tcttgcaagc gatctatcaa
    22081 ggccaatcat cgtattgatg atcattcagc tagacaactc ctctatcagc tttctcaatg
    22141 tgacaatccc tataactgtc ctcacggacg tcctgttttg gtgcatttta ccaagtcgga
    22201 tatggaaaag atgttccgac gtattcagga aaatcacacc agtctccgtg agttggggaa
    22261 atattaaaag tataaaaaag tctgggaaaa attttcaaaa tcagaaaaac gcataaaatc
    22321 aggtgttcaa aaaccttgat tttatgcgtt ttatcataga aatatttact tcattttttc
    22381 ctaattcttt tcgaaactct ttttaaacga cgtcagtttt atcagtaatc tcaaaacagt
    22441 gttttgagct aattttgcca gttttgtctg taacatcgaa gttgtgtttt accactctgc
    22501 gactggtttc ctagtttgct ctatgatttt cacagagtat taaattgcga ttttgccaag
    22561 tttctttatt cgtctaaaag tagagtctgt tctatgcgtc taatgtacga attaggttga
    22621 ccatttcaat agctccttgt gcacactcag aacccttatt tcctgcttta gtaccagctc
    22681 gttctatggc ttgttcaatt gtatctgtcg ttagcacacc aaacataaca ggaatttcgc
    22741 tatttaaact gatttgggcg attcccttag atacctcgct acatacataa tcataatgac
    22801 ttgtattccc tctaatgaca gctcccaagc agataattgc atcatatttt ttactttttg
    22861 ccatttttga tgcaatcagt ggtatttcaa aagctcctgg aacccaggct acctctatat
    22921 ctttctcgtt tacattctct cttttgagat tatctagtgc tccagataat aattttgaag
    22981 ttataaattc attaaatctc gctacaacaa tacctatttt aatattgttt gctactaaat
    23041 taccttcata agtgttcatt tatttttcct ccatatttaa aatgtgaccc attcgatttt
    23101 tctttgtttc taaataaaaa ctatcgtaag gattggcttc tatttcgatt gatattctac
    23161 tggaaatggt aattccatat ttttctaact gttcaacctt gtcaggatta tttgtcagta
    23221 aatgaagtga ctgaagtccc agatctttaa gcatttgtgc tccaatatga tattctctta
    23281 aatcaccttc aaagcctaat gcaagattgg catcaagcgt atccatgcct tgatcttgta
    23341 aatgataggc ttttaattta ttgataagtc caattcctcg tccctcctgt cgcaagtaaa
    23401 gtaagacacc cgaaccattc tcaacaatta ttttcatagc tttatcgaat tgctgtccac
    23461 aatcgcaacg taaagagcct aaaacatctc ctgttaaaca ttcggagtgg acccgacata
    23521 atacattggc ttcatcctct atgtttccca taataagagc aagatgatgt tccccattta
    23581 gtttatctat atagctaatt gctttgaaat taccgtatct agtaggcata ttgacagttg
    23641 aaactcgttc taccagctga tcatatgctt ttctatattc ttgtaattct ttgatggtaa
    23701 ttagtggaat gttgtgtttc ttcgagaact gaattaaatc atctattctc atcattttgc
    23761 catcatgatt cattatttca caacataggc cacactcttt tagtccagct aattttaata
    23821 aatcaacagt tgcttctgtg tgtccatttc tttctagaac accacctttt tttgcaatta
    23881 aaggaaacat gtgtcctggc ctgcgaaaat cagagggtgt tatatcttca gctacacaca
    23941 tacgtgcggt cagtcctctc tcctcggcag aaatacctgt ggtcgtttct ttataatcaa
    24001 ttgaaactgt aaaagcagtc ttatgattat ctgtattgtt ttcaaccata ggtgaaagca
    24061 ttaattgatt agctaaactt tcgctcatag gcatacaaat taatcctttg gcataagtag
    24121 ccataaaatt aacattttct gttgtagctg cttgtgcaga acaaattaag tctccttcat
    24181 tttctctatc cttgtcgtct ataacaagaa caagtcgtcc cttctgcaat gcttctaatg
    24241 cttcttgtat ttttcgatat tccattgact gattatcctt tctgctaaaa tccattttga
    24301 tataatagtt ccttcgatat ttctgatttt ggagagttat ccatcagttt ttgcacatat
    24361 ttacctaaga tatcattttc aagatttact gtactcccga cttgtttact cttaagaatg
    24421 gtttgtttca aggtatgagg gataacagat actgaaaagt ttactttgga gactttagcg
    24481 acagtcagac taatgccgtc aattgtaata gatccttttt caactattaa atctaaaatt
    24541 tctttttgtg tgttgatttg ataccataca gcattatcat ctttttttat tgacgagatt
    24601 tttcctgtac catcaatgtg tcctgtaacg acgtgacccc caagtcgacc gttgacagat
    24661 aaggctcttt ctagattcac ctcacttcca tgttttaata gagtaagagc tgttcgactc
    24721 catgtttcat tcattacatc aactgtaaag gattgatgat tgaaatgagt aactgtaaga
    24781 cagataccat ttactgctat actatcgcct aaatggatat ccgttaatat ttttgaggct
    24841 ttaattgata gtttacaatt acgagagtct ttctgtattc tttcaacttt tccgatttct
    24901 tcaattattc ctgtgaacat ggataaatca cttcactttc tatgagatag tcatttccta
    24961 tttgagaaaa agcataaggt ttcaatctaa tagcgtcatt tggcaaagaa atgccttcac
    25021 ctccgacagg aaacttggca ctgcctccaa aaatttttgg tgcaatatat attttcagct
    25081 catcaacaat ttgttgttcc aaagcactcc aattcattag actgccccct tctagaacta
    25141 ggctatcaat ctgcatgttt cctagatgtt gcattaaact cgataagtct atatgattgc
    25201 cttttttctt tatggaaagt atttcacagc catgattttg atatagcttc attttatttt
    25261 tgtcttcaga ggaagtggca atgtaagttt taatatcatt tgctgttttt acgattttag
    25321 aggtaagagg agttcgtaaa tgtgtatcgc atatgatacg gataggattt ttcccttcct
    25381 ccaatctaca tgtcagcaaa ggatcgtctt gaataacagt attgactccc accataattg
    25441 cactaacatg gtgtcgtaac tgatgcacat gctttcttgc ctcttcttca gtaatccatt
    25501 tggattgatt tgttttagtg gctatttttc catccattgt cattgcatat ttcataaaaa
    25561 catagggtac atgctgggta atatactttc taaaactttt tattaagtta agacactcat
    25621 tttctaaaat tccaacagta acttgaagat tattttcctc aagtatcttt actccttttc
    25681 cagatacaat aggattacag tctaggcttc caatgactac tcttgtaata ccactatcga
    25741 ttatagcatc tatacaagga ggtgttttcc cgaagtgaca acagggttca agtgttacat
    25801 aaagcgtcgc tccgacaggg gattctctac agtttttaag agcatttctc tcagcatgtg
    25861 agccaccaaa aaactcatga taaccttgtc cgatgatgtg attatctttt acaataactg
    25921 cgccgaccat aggattggga ttgacgtaac cagccccttt ttgtgccagt tttattgcta
    25981 atttcatata ttttgaatcg ctcatctcac tacctccaaa aaaatatacc ttgaatagga
    26041 gactactcaa ggcatacaaa agaaaactta tgcgattaac aaaaatgctc tgaaatgaca
    26101 agtaatcatt tcagagcacg caaaaagcac aaatatactt ttatcttctt tcatccagac
    26161 tatactgtcg gctttggaat ttcaccaaat catgcctttc ggctcgtggg ctataccacc
    26221 ggtagggaat tgcaccctgc cctgaagata gttattcaat tacagatgat tatagtactt
    26281 aattttgaat atgtcaacag ataaataccg attgtttttg atatactgta tttgtgataa
    26341 tcgattctcg ctcctcggat aaagaaaata tgatatacta gataaacgaa ataagagaga
    26401 aggaacacta tgtacgcata tttaaaagga atcattacca aaattactgc caaatacatt
    26461 gttcttgaaa ccaatggtat tggttatatc ctgcatgtgg ccaatcctta tgcctattca
    26521 ggtcaggtta atcaggaggc tcagatttat gtgcatcagg ttgtgcgtga ggacgcccat
    26581 ttgctttatg gatttcgctc agaggatgag aaaaagctct ttcttagtct gatttcggtc
    26641 tctgggattg gtcctgtatc agctcttgct attatcgctg ctgatgacaa tgctggcttg
    26701 gttcaagcca ttgaaaccaa gaacatcacc tacttgacca agttccctaa aattggcaag
    26761 aaaacagccc agcagatggt gctggacttg gaaggcaagg tagtagttgc aggagatgac
    26821 cttcctgcca agatcgcagt gcaagcaagt gctgaaaacc aagaattgga agaagctatg
    26881 gaagccatgt tggctctggg ctacaaggca acagagctca agaaaatcaa gaaattcttt
    26941 gaaggaacga cagatacagc tgagaactat atcaagtcgg cccttaaaat gttggtcaaa
    27001 taggagcaga gaatgacaaa acgttgttcg tgggtcaaga tgaccaaccc actctacatc
    27061 gcctatcatg atgaggagtg gggccagccc ctccatgatg accaagcgtt gtttgagttg
    27121 ttgtgtatgg aaacctatca ggcaggcctg tcttgggaaa cggtactcaa caaacgccaa
    27181 gctttccgag aagcctttca tagctatcaa attcactcag tcgcagagat gactgacact
    27241 gaattggaag ccatgctgga gaatccagct atcattcgaa atagagccaa gatttttgct
    27301 acacgcgcta acgcccaagc ctttctacag ttacaggcag agtacggctc ttttgatgcc
    27361 tatctttggt cttttgttga ggggaaaact gtcgttaacg atgttcctga ttatcgccaa
    27421 gccccagcta aaacaccctt atctgagaaa ttagccaaag atctcaaaaa acgagacttc
    27481 aagttcacag gcccagtcgc cgtattgtct tttctacagg ctgcagggct agttgatgac
    27541 cacgagaatg attgtgagtg gaaaggtctt aaatgatgtc taacaaaaat aagggaattc
    27601 tgatttttgc gattctctat acagtcctct ttgtgtttga tggcgttaaa ttgctggctt
    27661 ctttaatgcc atctgccatt gcaaattatc ttgtttatgt agttttagct ttatatggct
    27721 ccttcttgtt caaggataga ttgatccaac aatggaagga gattagaaag actaaaagaa
    27781 aattcttctt tggagtctta acaggatggc tctttctcat tctgatgact gttgtctttg
    27841 aatttgtatc agagatgttg aagcagtttg tgggactaga tggacaaggt ctaaatcagt
    27901 ctaatattca aagtaccttt caagaacaac cactactgat agctgttttt gcttgtgtca
    27961 ttggacctct ggtagaagaa ttatttttcc gtcaggtctt attgcattac ttgcaggaac
    28021 ggttgtcagg tttactaagc attattctgg taggacttgt ttttgctctg actcatatgc
    28081 acagtttggc tctatcagag tggattggtg cagttggtta cttaggtgga ggccttgcct
    28141 tttctattat ttatgtgaaa gaaaaagaga atatctacta tcccctactt gttcacatgt
    28201 taagcaacag cctctcctta atcattttag ctatcagtat agtaaaatga aatgagaaca
    28261 ggacaaatcg atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatat
    28321 actgtaatat gtgatgaaaa tgccagtaat gataccgaga aaaaagctga gaaacttttc
    28381 ccagctttat ttgttatagt caaagagaat gacttgttcc tgtgcatcta catgagcatg
    28441 gaccccaaag ggtacaattg ctcttggagt tgcgtggccg acattcagat tatagacaat
    28501 cgggatattg ctgtcaatga tatccaatag tgcctcttta tagtcgtcat ggaaagtttc
    28561 atccataggt tttccgacca agagtccatt gatgaccgcg aatatgccag tgtcctttaa
    28621 agttagcaac atctttttga agtcttctgg cttaggcttt tcttcgcttg tttcgagcaa
    28681 gaggattttc ccttcccagt ctgacaagtc agggaaaagt ttgtattttt ggcagagttc
    28741 cgtgctatct gcgtatcgag agttgtcaaa gatatcgtag agggattcga ggcaaccacc
    28801 gaggattttc ccctcgaact gagcacttcc ttgcaacaag tcaaaacctg tatttgtatg
    28861 actgacacga ggtgttccca gggccgtggg actaaaatca gttcgttcct cataccaaac
    28921 gtcactaggg cggatttctg aaattcttcc cgtctcaatc aattctttaa agtagtgaag
    28981 gctataggct agcatttctt tgtctaattc acaaatgtct gctaaaaagg attgaccata
    29041 aaaagtcttg attcctaatt tatgcaacat gaggtggttc atggttgtat ccgagaagcc
    29101 aagaaaaatt ttttgtttga taaccttttg gagttggtca ttttcaaaaa gataaggtag
    29161 caagcgatag gtatcgtctc caccgatggc acataggatc atgtcgatgc tatcatcaga
    29221 aaaggcatga atcaaatcct ctgcacgagc ttcaggatgg tccttgataa agtctaatcc
    29281 ttttaacgaa tggggcaaaa agatgggatt gagtcccaga tccttgagac gttggatacc
    29341 caagtccact tcgtgtttga caaaatcctc tccgataatg ccactagata aactaacaat
    29401 accaatagta gaaaccatat ctcatcctcc tagaaataga ttgagcctat tttatcacaa
    29461 aatataagag aaagctatgt gggatttcag aagaatactt ttaatactct tcgaaaatct
    29521 cttcaaacca cgtcagtttt atctgcaact tcaaagctgt gctttgagca acctgcagct
    29581 agcttcctag tttgctcttt gattttcatt gagtacaact caaaaaagta agtgaaaaag
    29641 caaccgcacc tgcagttgct tttatcgttt tcttattctt gatagcgtgt tggtcctgca
    29701 cttgcactac ggatattcaa gcgtttcttg agatagaagc gaagggctag gagggctgct
    29761 ccaataatag ctagtggcaa tggatccagt actgggttaa ggctagctgg taggaagctt
    29821 gttgcaaaga agacaagcaa ccaaaggaac atagaagcta ggataactag tacagatttc
    29881 cagaaaggtg gacgttgact gcgatccata tctggtccat agtattggta aacaaagtag
    29941 tacatcaagt agaaggcaaa tccaccaacc agtccaacta atagaagagt aatcaatcca
    30001 tagccgaaag cttgatctgc cgcaaagaag gttgtgaggg cgctgacaag ggcaaagagg
    30061 ctagtgatga aaagagctga gtccataatc atcagttttg gatcatcatt ttcttttgga
    30121 tgctcttttt cgtactgctc tttgacagtg aagctatgag cccaatgagt tggtgcgccg
    30181 tatagggaac gggcagttgt acctttagat tgctcctcaa ggatttgggg aatgacttcc
    30241 tcaaaaatag tctggatttc agcgtctgtt ttcccatctt tgatgaattg ttgggtagca
    30301 atgtggacaa actcttggtt tttcttagtt aatttttgta gatcaatttg agacatagga
    30361 actcctctta gaaccatttt ttatggatga gatagagagt gagcgagaca ctcatagcaa
    30421 aggcgataaa gacgattaac cagaaggcat ttggctctcc gtttagggga atttcattat
    30481 ccttaaagtt catcccgtag gcagaaaaga ccatggttgg gatggacatg acgatggtca
    30541 caagggccaa ggttttcatg atgttgttct ggttgttaga aatgatagag gcaaaggtct
    30601 ctgtcataga atgcaagacg tttccataaa tatctgccat ctcgatggcc tgttgggttt
    30661 caatcagggt gtcttcaagc aggtcttcgt cctcaaggta tttcttgata ttgctggttg
    30721 aactggtcaa tttcttaatc acgcgctcat ttgttttgag ggaggccttg aaatagacga
    30781 tagttttttc caattccatg agctcaatca attcttcatt acgagttgat tgatgcagtt
    30841 gactttcgat ttgttcactc ttgcggtcga ttgaacgaag ggctgttagg taaagctctg
    30901 cattgcgata aagaatttga aagataaaac gtgaacgcat gaaggtatag aaattacgca
    30961 atcgacggtt gataaagaca tcaaggacag gtagtggttc caaacacgta gtgataatgg
    31021 tttcctcagt gatgataata ccaagcggga tggttacgta gtaggtgcgg ttatttcttt
    31081 cctccgtgac cggcacgtct acgataatca gggtatactc gtcttcaatg gtaatacgag
    31141 acatttcttc cgcatcgagc ggtgctcgaa ggtcggcaat atcaatatcg aaggtgttgg
    31201 cgatttcgag tgattcattt tgagtcggat tgacgagatt gatccaagta cccggttcaa
    31261 gcgtatcgat ctctttaaat tcagttgttg tagagagaaa aacttgtttc atatccctta
    31321 tcctttctca tttttcagat tttttcacac tgtactatta tactacaaaa tcggcctttt
    31381 gggtaataga atctcacttt ttttggtata atggtaagca ataatggact aggaagaaca
    31441 aagatgcaag ataaaattgt cattcatggg gcgcgtgccc ataatttaaa aaatattgat
    31501 gtggagattc cgcgagacaa gttggttgtc gtgactggct tgtcaggttc agggaagtcc
    31561 agtctggctt ttgatactct ctatgcggag ggacaacgtc gctatgtaga gagtttgtcc
    31621 gcctacgctc gtcagttctt gggaaatatg gaaaaaccag atgtagatgc cattgatggt
    31681 ctcagcccag ctatttccat cgaccagaaa acgactagta aaaatcctcg ctcgacggtg
    31741 ggaacgacga ctgaaatcaa tgactatctg cgtctcctct acgcacgtgt ggggacgcct
    31801 tactgtatca acggacatgg agctatcaat gcttcttctg tggagcaaat cgttgataag
    31861 gttttggagt tacctgaacg ccagcgcttg cagatcttgg ctcctgtcat ccgcaagaaa
    31921 aaaggccaac ataagagtgt tatcgagaag gttcagaaag acgggtatgt tcgtgttcgt
    31981 gtggatgggg aagtctatga tgtgaccgaa gtgccagagt tgtctaagag caagcaacac
    32041 aatatcgatg tcgtggttga ccgtattgtt atcaaggagg gcattcgtag tcgtctcttt
    32101 gattccattg aggctgccct tcgtattgca gaaggttatg tcattatcga caccatggac
    32161 gattcggagt tgctcttctc tgagcattat gcctgtccag tttgtggttt tactgtccca
    32221 gagttagagc ctcgtctctt ctctttcaat gctccttttg gctcttgtag tgagtgtgac
    32281 ggcttgggca tcaagctgga ggtggatact gatttggtag tgccagatgc cagcaaaacc
    32341 ttacgtgagg gagcgctagc accttggaat cctatctcat ccaactacta tccaaacatg
    32401 ttagagcagg ccatgaaagt ctttggagtg gctatggata agccttttga ggacctgtca
    32461 gaagaagata agaacttgat tctctatggg tcagatggta aggaattcca tttccactat
    32521 gagaatgaat ttggtggcgt gcgcgatatc gacattccgt ttgagggagt tatcaataat
    32581 atcaaacgtc gttaccatga aaccaatagc gattacactc gcactcagat gcgtctctac
    32641 atgaatgagc tgacctgcgg aacctgtcaa ggctatcgtc tcaatgacca ggccttgtct
    32701 gtccgtgtgg gcggccagca agggccacat attggagaaa tctcagacct gtctatcgct
    32761 gaccacttgg acttggtgag ccagttaact ttgtctgaaa atgaagccat cattgctcgt
    32821 cccattctca aggaaatcaa ggatcgtttg accttcctta ataatgtggg tcttaactat
    32881 ctaaccctgt cacgttcagc aggaaccctt tcaggtgggg aaagtcagcg cattcgtttg
    32941 gcaacccaga ttggttccaa cctatcaggc gtcctctata tcctagacga gccgtcaatc
    33001 ggtcttcacc agagggacaa tgaccgcctg attgccagtc tgaaaaagat gcgtgacttg
    33061 ggcaatactc ttatcgtggt ggaacacgac gaagatacca tgcgtgaagc tgattatctg
    33121 attgacgttg gtcctggtgc cggtgttttt ggtggtgaga ttgttgcagc aggtacacct
    33181 aagcaagtgg ctcgcaacag caagtctatc acaggtcagt acttgtcagg caaacgtgtc
    33241 atcccagtac cagaagagcg ccgtgtcgga aatggtcgtt ttattgaagt gataggagcg
    33301 cgtgagaaca acttgcaaaa tgtcacggct cgctttccac taggaaaatt tatcgcagtg
    33361 acaggtgtgt cgggttcagg gaaatcgact ctaatcaaca gcattctcaa aaaagccatt
    33421 gctcagaagc tcaaccgtaa ttcagacaaa cctggtaagt ttaagactat tacagggatt
    33481 gagcatgtcg accgtttgat tgatattgac cagagcccta tcggacggac gccgaggtct
    33541 aacccagcta cctatacagg agtttttgac gatatacgtg acctttttgc tcagacaaat
    33601 gaagccaaga ttcgaggcta caagaagggg cgcttcagtt tcaacgtcaa gggaggtcgc
    33661 tgtgaagctt gctcaggtga cgggattatc aagattgaga tgcacttctt gccagatgtt
    33721 tatgtggctt gtgaagtttg ccacggaacc cgctataaca gtgaaaccct agaagttcac
    33781 tacaaggaaa agaatatttc gcaggtcttg gatatgacgg tcaacgatgc ggtagaattt
    33841 ttccaacaca ttccgaaaat tcaacgcaaa ctccagacca tcaaggatgt agggctaggc
    33901 tatgtgacct tgggacagcc agctaccacc ctttctgggg gagaagccca acgtatgaag
    33961 ttagctagcg aactccacaa acgctcgaca ggaaaatctt tctacattct ggatgagccg
    34021 acgacagggc ttcatacaga ggacattgct cgcctgctca aggttttagc tcgctttgta
    34081 gacgatggca atacagtcct cgtcatcgag cacaatctgg atgttatcaa gacggcagac
    34141 catatcattg acttgggacc tgagggcggt gtcggtggtg gaaccatcat cgtaacagga
    34201 actccagaag aagtagctgc caacgaagcc agctatacag gacactattt gaaaggaaag
    34261 ttacatcatg aataaacgcg tacaagcatt tctagttaaa atgcaagaaa aagaactaga
    34321 tggtgtcatc atcaacaatc ttaaaaacgt ctattatttg actggttttt ggggctcaaa
    34381 cggaacagtc tttatcagcc gtgaccgtca ggtcttagtg acagactctc gctatatcat
    34441 cgcagctaag caagaaacca gtggttttga gattgtggct gatcgtgatg aattggctgt
    34501 cattgcagga attgttaagg acatgggctt gactcgtatc ggttttgaag atgagatttc
    34561 agtgtcttat taccaccgta tgcaggcagc ttttgcaggt ttggacttgt ttccgcaaac
    34621 tcagtttgtg gaaggtcttc gtatgattaa ggatgaggca gagattgcag cgattcgcaa
    34681 ggcttgttct atctcagacc aagctttccg cgatgcgctt gactttatca aaccaggaaa
    34741 aactgaaatt gagattgcca acttccttga cttccgcatg cgtgagttgg gagcatctgg
    34801 cttatctttt gatacgatcc tagctagcgg tatcaattct tctaaacccc atgcccatcc
    34861 aatgcacaaa ccagtggagt tgggagaagc cattaccatg gacttcggct gtctctatga
    34921 ccactatgtc agtgatatga cccggactat ctatctaggg catgttagcg atgagcaggc
    34981 agagatttac aatacggttc taaaagctaa ccaagccttg attgaccaag ctaaggcagg
    35041 cttaggtttc cgtgactttg acaaaatccc tcgtgatatt atcattgagg caggttatgg
    35101 cgactacttt actcacggca ttggccacgg tattggtctg gatatccatg aggaacccta
    35161 ctttagccag acttctacag aaactattaa gacaggtatg gccttgaccg atgaaccagg
    35221 tatctatatc gaaggcaaat atggcgttcg tatcgaggat gatatcctga ttacagagac
    35281 aggttgtgaa ttattgaccc tagctccaaa agagttgata gtcatttagt tgtttgtcaa
    35341 gaaaaaagta cgaaaatgac gaaaaaagtc aaaaaaaatt aaaaataggt cgcaagtcgg
    35401 atgtttttta tggtataata gactaaactg atagtaacat gtagcgaaag gggtaggtac
    35461 atgattaaaa tttatacagt ctcaagttgt actagctgta aaaaagcaaa aacctggctc
    35521 aatgcccacc agttaagtta taaagaacaa aaccttggta aagaaggaat tacgagagaa
    35581 gaattactgg atattctaac caaaacagat aacggaatag ccagcatcgt ttcgtctaaa
    35641 aatcgctatg ccaaagccct tggagtggat attgaagatt tgagtgtcaa tgaagttctc
    35701 aatctgatta tggaaacacc gagaatttta aagagcccaa tccttgtaga tgaaaaacgc
    35761 ctgcaagttg gctacaagga agacgatatt cgtgccttcc taccacgctc tgtccgtaat
    35821 gtagaaaatg cagaagcacg tttacgtgca gctctataaa catcaaggct gggagcaatt
    35881 cccagtctta ttctatttta atttcaaaaa gaaagaagaa agaaatgaaa aaaatagttc
    35941 ttgttagtct agctttcctt tttgtcctgg ttggttgcgg acagaaaaaa gaaactggac
    36001 cagctacaaa aacagaaaag gatacgcttc agtcggcatt gccagttatt gaaaatgccg
    36061 agaagaatac agttgtaacc aagactttgg tcttgcccaa gtcagatgat ggtagccagc
    36121 aaactcaaac cattacatac aaggacaaga cttttttgag cttaacgatt caacaaaaac
    36181 gtccagtttc tgatgagttg aagacttata ttgaccaaca tggagtggaa gaaactcaaa
    36241 aagctcttct tgaagcggag gagaaggata agtctatcat tgaagctcgt aaattggtag
    36301 gtttcaaact tgaaacaaaa ctattgagcg caacggaact tcaaacaacg actagttttg
    36361 attttcaagt tctggatgtc aagaaggctt cccagttgga acatctgaag aatattggtt
    36421 tggaaaatct tttgaaaaat gaaccaagca aatatatttc agacagattg gcaaatggtg
    36481 cgacagaaca gtagaaaatc caaataaata gactgcctga attgtagaag gtaaggaatt
    36541 atgctataat ggtactattc taaggaaaga gggtgttaga atgggattta ctgaagaaac
    36601 agtacgtttt aaattggacg attccaataa aaaagaaatt agcgaaactt tgacagatgt
    36661 ttatgcttcg ttgaacgata agggttacaa cccaattaac caaatcgtag gttacgtatt
    36721 gagtggagac cctgcctacg ttcctcgtta taataatgca cgaaatcaaa tccgtaagta
    36781 tgagcgtgat gaaatcgttg aggaattggt tcgctactac ctcaaaggac aaggagtcga
    36841 tctataacct atgagaatta tgggattgga cgtcggttca aaaacggtag gggtggcgat
    36901 tagcgatccg cttggtttta cagctcaagg gcttgaaatc atccagataa atgaagaaca
    36961 aggccaattt ggttttgacc gcgttaagga attggttgat acttacaagg tggaacgatt
    37021 tgtagtgggc ttgcctaaaa acatgaacaa tacaagtgga ccgcgcgtag aagctagtca
    37081 agcctacgga gcaaagctag aagagttttt tggtttacca gtagactatc aggatgaacg
    37141 cttgacaaca gtggctgctg agcgcatgtt gattgaacaa gcagatatca gtcgcaataa
    37201 gcgcaagaaa gtcattgata agttagcagc tcagctgatt ttacaaaatt atttagatag
    37261 aaaattttaa tataaaggag aggctatgtc acacgatcat aaccacgacc acgaagaacg
    37321 tgaactaatc acactagtag atgagcaagg aaatgaaacc ttgtttgaaa tccttttgac
    37381 aattgatgga aaagaagaat ttggtaaaaa ctatgttctt ctagtaccag ttaacgcaga
    37441 agaagacgaa gacggacaag ttgaaatcca agcttactca ttcatcgaaa acgaagatgg
    37501 aacagaaggc gaattgcaac caatcccaga agactcagaa gacgaatgga acatgattga
    37561 agaagtcttc aacagcttta tggaggagtg atacagtctg ggagactgta tcagcccaac
    37621 tcccagaaat ggggaagagt tggaacatcc agtggatgtt tttagcccag ttcctttaaa
    37681 taagagagaa ctggtaagtc cgggggacgt ttttagcctg acgctaaaaa taaaaacagt
    37741 cagtaagttc gggggatgtt ttagcccagc tccttgaaat aagatagagc tggtaagtct
    37801 gagaaactgt atcagcccaa ctcccagaaa tggggaagag ttggaacgtc cagtgaacat
    37861 ttttacccca actcttagaa attgagaaga gttggaacat ctagtggatg tttttagccc
    37921 atattcacat tcatagtagc tagttattag ctaaccaggt aagaggtcgg gacgaaaatc
    37981 ctggccttgt ttttattagt aaccatactt taatgcgata attgattgga cttttgtcaa
    38041 ggagatgtgt atgtttgaag tagaagaatg gctccatagt cggattggtt tgaattttcg
    38101 atcaggtttg ggccgaatgc agcaagcggt ggatttgtta ggaaatcctg agcagtctta
    38161 ccctattatc cacgtaacag ggactaatgg gaaaggatct accattgctt ttatgaggga
    38221 attatttatg gggcatggca aaaaagttgc gacctttacc tcccctcata tcgtctctat
    38281 caatgaccga atctgcatta atgggcaacc tatagcagac gcagacttta tccgtttgac
    38341 tgatcaggtc aaggagatgg agaaaacgct tctgcaaact cctgcccagt tgtccttttt
    38401 tgaattgctg accttagttg cttttcttta ttttagggag caagaggtgg atttggtttt
    38461 attagaagtg ggaattggtg gcttacttga cacgaccaat gtggtaactg gagagcttgc
    38521 tgtcatcacc tccattgggc ttgaccatca agaaaccttg ggtgatagtc tagaagcaat
    38581 tgcagagcag aaagctggta ttttcaaggc tggtaaaaag gcagtgattg cgaaattgcc
    38641 tccagaagct agacttgtct gtcagaaaaa agctgaatct ttagctgttg acctttatca
    38701 ggcaggtcaa gatttttcag tgctgaatgg tgatttttca agctctttac taaatctttc
    38761 acagctgaac ataggcttag aaggagccta tcagcaggag aatgcggcct tggcgttgca
    38821 aacttttctt ctttttatgg gagaaagaaa ggaagctatt gatgaacagg ctgtaagaaa
    38881 ggccttggaa cagacccatt gggctggtcg cttggagcgt attcgcccac agatttactt
    38941 ggatggtgct cataacctcc ctgccttgac tcgcttggtt gagtttatca aagaaaaaga
    39001 gcaggaaggc tatcgacctc aaatcctctt tggatccttg aaacgtaagg attatcaagg
    39061 gatgttgggt tatctgactg aaaatttacc tcaagtggaa ctcaaaatga ccggctttga
    39121 ctatcaaggg tctttggacg agacagatgt aacaggttac catgtaattc cctcttaccg
    39181 agaatttatc agcagtttcg aagaaagggc agacgctcaa gacttgctgt tcgttacagg
    39241 gtctctctat tttatctcag aagtacgggg ctacctgctg gaccgtgagc agataaattg
    39301 acccccttgt ttgaacttgt tatactaggg gaattgctgg ttagagaaaa tttctctaaa
    39361 ttggtagcag aaaggaaatt catcatgaaa ttaaaaagat tcacactttc tcttgcttct
    39421 ctagcaagtt ttagtctctt agtagcttgt tcacaaagag ctcaacaggt tcaacagcct
    39481 gttgctcagc agcaggtcca acagcctgct caacagaata ccaatactgc aaatgcagga
    39541 ggtaaccaaa atcaagcggc tccagtacaa aaccaacctg ttgctcaacc gaccgatatt
    39601 gatgggactt atactggtca ggatgacgga gaccgtatca ctttagtggt aactggaacg
    39661 actggtacat ggactgagct cgaatctgac ggggatcaga aagtcaaaca ggttacattt
    39721 gattcagcaa atcaacgcat gattattggc gatgatgtca aaatttacac tgtaaacggt
    39781 aatcaaatcg tcgtagatga tatggataga gacccatcgg accaaatcgt tttaactaaa
    39841 taagactatg caagtagaaa gggctggatt tttcagcctt tttactttta cctagaaaat
    39901 aatcataaat acttgccttc taccgaatac ctgcgttata ctagtatcaa ttacttgttt
    39961 ttagcattca aactatcaag gaggggatat gaaatatagg aaatttcaat tattgatgtc
    40021 caagtatggc tttagtcttt cgattatgct acttgaactt tgtcttgttt ttggtctctt
    40081 tctttattta ggacgcatgg ctcccatttt atggattact gtcctcattc tactgagtat
    40141 catcacaatc atttcgatag tcaaccgtaa tacgactcct gagaataagg taacctggtt
    40201 gttagtagcc tttgtgccag tatttggtcc cttgctctat ctgatgtttg gtgaaaggcg
    40261 attgtccaaa aaagaaatca aacaactgaa gaagctaggc tctatgcatt tccaagaagc
    40321 aaatagccag ctactaaaag agaaattaaa agaaagtgac aaggcagctt atggagtcat
    40381 caagtcctta ttgagtatgg ataccaatgc tgacatctat gatcaaactg cctctacatt
    40441 ttttcctaac ggagaagcta tgtggaaaaa gatggtagaa gatcttaaaa aggctgagaa
    40501 atttattttc ttggaatatt acattataga agaaggtttg atgtggaatc gcatactaga
    40561 tatactagag caaaaggtag ctcagggtgt agaggttaag atgctctatg atgatatcgg
    40621 ctgtatggct actttaacag gagattatgc acatcgactt cgtcagctgg gcatcgaggc
    40681 ccataaattc aataaagtta ttcctcgttt gacagtggct tataataaca gagatcatag
    40741 aaaaatattg attgttgatg gtcagatagc ctatactggt ggggtcaatc tggcagatga
    40801 gtacattaac cacgtcgaga gatttggtta ttggaaggat agtggaattc gcttagacgg
    40861 actagcagta aaagctctga cacgcttatt tttgaccact tggtacatta atcgaggaga
    40921 aattagtgat tttgatcaat atcatttaga aaatcattct atcccgagtg acggtttaac
    40981 cattccatac ggaagtggac ccaagccaat ttttcgagcg caggtaggga aaaaagttta
    41041 tcagagttta atcaatcaag caacagaatc ggtctatatt acgacacctt atttgattat
    41101 agattatgat ttaacagaga caatcaaaaa tgcagctatg agaggggtcg atgttcgaat
    41161 tatcacccct tacataccag ataagaagtt cattcagtta gtcacgagag gagcttatcc
    41221 cgaccttctt tctgctggtg ttcggattta tgagtatagt ccaggtttta ttcatagtaa
    41281 gcagatgttg gtagacgaag attttgcggt ggtggggaca atcaatctcg actaccggag
    41341 cttggtacac cattatgaaa atgcagtctt actctataaa actccttcta taagggaaat
    41401 cgcccgagat tttcgaaata tatttgcaga ttctcaggaa gtctatcctc attctatcaa
    41461 aacgagctgg tatcaaaagc ttgtaaaaga aatcgcccag ctattcgccc ctatcttata
    41521 agaaatctag aacttaggat acaaaccaga cctatttttc ttgtctttta cgaatagaaa
    41581 gaaagaggag gggaattcat gctaactaag gaagaagtga atgatatgat tgagttcaaa
    41641 cagacacatt tacaccgctt tcgggaaatt gaaacctttg tgaaactatg caaaaaatca
    41701 ctcaaacagc catcgcaagc tgacaatcct aggacttttt gatatactaa gacagataaa
    41761 ttgaacagga gaaacgatat gagtgtgtat ggtagagtag aagaagttca taaggagaat
    41821 cgtgaacctc tagaatacca aatcgaacaa gaatcgcatc atcgtgaatc aagtcgtctt
    41881 cctttggtga aaattttact atggagtacg cttgtaacgg ggataactct aggagtaccg
    41941 ctattactcg acttaatgag tgcacaagaa gtgcaggatt tttatgcagg ttgggcgctt
    42001 catcagacag ggaagattta cagcgactat tatggaagtc aaggtttgct ttattatttg
    42061 ctgacttacg tgagtcaggg cggatttttc tttgccatct ttgagtggtt agccttggta
    42121 gcaggaggat ttttcctttt tagatcagcg gacaccttga cagagcaagg agaccaagct
    42181 ggacatctgg tgactatttt ttacatgcta gttacaggtc ttgcttttgg tggaggctat
    42241 gcgactcttt tagcgcttcc tttcttattc gcagccttta gtttagttgc ggcttaccta
    42301 agcaatccaa gccatgataa gggatttgta cggattgggc tagctttggc aggcggattt
    42361 ttctttgctc ccttatcatc gctcctgttt attgctgtag tgagtttagg cttgttggtc
    42421 tttaaccttg ggcatagacg ctttgcgcat gggttttatc agtttcttgc agtggcttta
    42481 ggtttttcac ttgtctttta tccaactgcc tactatagtg ctgcaacagg aagttttggg
    42541 gatgcgatta gtggtattcg ttatcctatt gacagtattc gctttgattt tacttctaaa
    42601 attttagaga atatgttttt ttatggcttg ttgtcccttg gtttgggatt tgtggtttgt
    42661 atctttttag gtctctttca atccaaaccc tttaaactat acgtcatttc ggttcctgca
    42721 agtcttgtgg taattttagg tttgatttta cttttctttt cacaagagcc tctgcacgct
    42781 tcttatttga tggacgtctt ccctgttttc ctacttttat tggtaaccaa tattaagagt
    42841 caacagaggg ggcgtagtgc tagaagaagc cgaagagaaa cgccagttag cctatggagt
    42901 cgtttcttca aaggaaatct atatctgcta gtttttgggt ttgtctatct tttgtctgtt
    42961 ccttttttga tgaagtttgt cctttatcca gtaccttatc aagaacgtaa tcgtcttgct
    43021 gatttggtaa aagaggagac aaatacggaa gatgctatct atgcatggga tgatactgcg
    43081 actctttatc gtaagagtga gcgcttgtcg ccatcggcga ttttgtcccc gttgcactat
    43141 acagcaactg aggaaaatcg taataagtta cttaatgact tgaaagaaaa acaacctaag
    43201 gtgattgtgg taaatgacaa ggtggtagtc tggtctgaag tggaaacact cttaaaagaa
    43261 aattaccaac aagtaaagac tgattactca gagtttaaag tctataaaat taaataacca
    43321 aatcaatatc ttgtgtattt ttaaaaattt taggattttt aacacaagat attgattttt
    43381 ctttttagag tggtataata ctttttagaa agaacatttt agaaaagagc atgcgtatga
    43441 ttgcactaga agaaaaaatt acaattttgc caactctctt cgtcgagaaa cgagatggga
    43501 gacgtgttgt atttgatgtg gacaagattg acaaggctct ccacaaggcg gctgacaagg
    43561 ttatggatgt gacacccctg gttgaaaaac gcctcaatga tctgactgag cgaattatta
    43621 cagaaattca tagtcgcttt ccacagggaa ttaagattta cgaaattcaa aatatcgtag
    43681 aacatgaact ccttgaagcc aaagaatatg cactggctga ggagtatatt acttatcgga
    43741 cacagaggga ttttgagcgc tcaaaagcga cggatatcaa ctttagtatt cataaacttc
    43801 tcaacaaaga ccagacagtt gtcaatgaaa atgctaataa agacagtgat gtctttaaca
    43861 ctcagcgtga tttgacagca gggattgttg ggaaatcaat cggactgcaa atgcttccta
    43921 agcacgtagc caatgcccac caaaaggggg atatccacta tcacgatttg gactacagtc
    43981 cctatacccc tatgaccaac tgctgtttga ttgattttaa gggtatgttg gaaaatggtt
    44041 ttaagattgg aaatgcagag gtagagagtc ccaagtctat ccagactgcg acagcacaga
    44101 tttctcaaat cattgccaac gttgcttcta gccagtacgg tggctgttca gctgaccgta
    44161 tcgatgaaat tttggcgcct tatgcagaga agaactatca aaaacatctc aaagatgcag
    44221 aagagtgggt attgcctgaa aaacgggaag attacgcttg gaagaaaacg caaaaggaca
    44281 tctacgatgc catgcaatct cttgagtatg aaatcaatac tctcttcact tcaaatggac
    44341 aaacaccttt tacttcgcta ggttttggtc tgggaaccag tcgttttgaa cgagaaattc
    44401 aaaaagctat tttaaacatt cgcatcaagg gtcttggttc agaacaccgt acggctatct
    44461 ttcctaaact tattttcact ctgaaaagag gacttaattt agaggaagga actcccaact
    44521 acgacatcaa gcagttggct cttgagtgtg caaccaaacg gatgtatcca gatgttttat
    44581 cttacgataa gattgttgac ttgacaggat ccttcaaggt gcctatgggt tgccgttctt
    44641 tcctccaagg atggaagggt gaaaatggtg tagaagtcaa ttcaggtcgc atgaatctgg
    44701 gtgttgtgac ggttaatctg cctcgtatcg ctcttgagtc tgaaggtgat atgaataagt
    44761 tctgggaaat cttcaacgag cgaatgaata tcgcagaaga tgctcttgtt taccgtgtcg
    44821 aacgcactaa agaggcgaca ccagcaaatg ctcctattct ttatcagtat ggtgcttttg
    44881 gccatcgtct aggtaaagaa gaaagtgttg accagctctt taagaatcgt cgtgcgaccg
    44941 tttcgctggg ctatatcggc ttgtatgaag tagcgacagt tttctttggt aacagctggg
    45001 aaagtaatcc agatgctaag gaattcacgc tagacatcat tcacgatatg aaacgccgtg
    45061 tagaagagtg gtcagaccaa tatggctacc atttctctat ctactcaaca ccatccgaaa
    45121 gtctgacaga ccgtttctgc cgactagata tagacaagtt tggctctatt cctgatatca
    45181 cagacaagga atactacacc aactctttcc actacgatgt tcgtaaaaat ccaacaccgt
    45241 ttgaaaaatt ggactttgag aaagtctatc cagaagcagg tgcgtcaggt ggtttcatcc
    45301 attattgtga gtatccagtc cttcagcaaa atccaaaggc cttggaagct gtctgggatt
    45361 atgcttatga ccgtgtaggc tatctaggca ccaatactcc gattgaccgt tgctacaagt
    45421 gtgactttga aggggatttt gaaccaactg agagagggtt tgcttgtcca aactgtggca
    45481 atagcgaccc taaaacagta gatgtggtga agcgaacttg tggctaccta ggtaatcctc
    45541 aagcaagacc gatggtcaac gggcgtcaca aggaaatcgc tgcgcgtgtc aaacatatga
    45601 atggttcaac gattaaaata gctgggcatc aagtaacaaa ttagaaagaa atgaaatggg
    45661 aaaatatcaa ctagacgata aggggcgcgc acaagtgacc cgttatcacg agaaacactc
    45721 taaaggtgga gctggtaaga aagaacgctt gcttagcttc agagaacaat ttttaaacaa
    45781 gaacaagaaa aaataaaagt gagagccagc tctcgctttt ctcatagtgg gaggtaagga
    45841 tggaattacg cagaccaaga ttagcggata agaaagctgt tttagatatg atgacagagt
    45901 ttgaaaaatt tcagtcgcct cacgacggcg gtttatggga tacagagaac tttgtgtatg
    45961 aagactggtt agaaagcaat caggaacagg aaatggggat taatctgcct gaaggatggg
    46021 ttcctgcaat tcagttagtg gctttttctg agaaaggtca agcagttgga tttcttaatc
    46081 tccggttgcg cctcagtaac tttctactag aagaaggtgg ccacattggc tactccattc
    46141 gtccatctga aagaggcaag ggttatgcaa aagagactct ccgtcagggc ttgcaagttg
    46201 ctaaggaaaa gaacatcaag aaagctctgg tgacctgtag cgtgaataat cctgctagca
    46261 gagcagtcat tctagcaaat ggtggaatat ttgaggatgc tcgcaatgga gtcaagcgtt
    46321 attggataga ggtagcgaat gaataatcca aaaccacaag aatggaaaag cgaggaactt
    46381 agtcaaggtc gtatcattga ctacaaggcc tttaactttg tggacggcga aggcgtgcgt
    46441 aactctctct atgtatcagg ctgtatgttt cactgcgagg gatgttataa tgtagcgacc
    46501 tggtccttca atgcaggcat tccctataca gctgagttgg aagaacagat tatggcagat
    46561 cttgcccagc cttatgttca ggggttaacc ttactaggag gggagccttt tcttaatact
    46621 gggattctct tgccacttgt taagcggatt cggaaggaat tgccagacaa ggacatctgg
    46681 tcctggacgg gctacacttg ggaagaaatg atgttggaaa ctccagataa actggaactc
    46741 ttgtcactga ttgacattct tgtcgatgga agatatgatc gaactaagag aaatcttatg
    46801 ctccagtttc gaggttcgtc caaccaacga attatcgatg tgcaaaaatc gctcaaaagt
    46861 gggcaagtag tgatttggga caagctcaat gacggaaaag aaagctatga acaggtgaag
    46921 agagaatgaa gaaaaaagac ttagtagacc aactagtctc agagatcgag acggggaaag
    46981 tcaggacact gggaatatac ggtcatggag cttcaggtaa atcaaccttt gcacaggaat
    47041 tgtaccaagc tttagattct actacagtaa atttgctaga gacagatcct tatatcacct
    47101 caggacgcca tctggtagta cccaaggacg cgccgaatca aaaggtgaca gccagtctgc
    47161 cagtggcgca tgaactggag agtttgcaga gagatatcct tgccttgcag gcgagtatgg
    47221 atgtcttgac aattgaagaa ccttggaagg ctagtgaggt tttgtctgga gccaaaccaa
    47281 ttttgattgt cgaagggatg tctgttggct ttctacccaa ggaactcttt gaaaaaacca
    47341 tctgtttcta cacggatgag gagaccgaat taaagcgacg ccttgctaga gatacgactg
    47401 tgagaaatcg cgatgcatcc tttatattgg ctagccatca gatgagacgg gagcagtatc
    47461 tgcgatacta taaagaaact gagtctaagg cggatatctt agtggaccaa tcagaagata
    47521 aatttgatgt caagaggact taacttatgt atagaagaaa tatagaagaa aatccctaat
    47581 tttagaaaga aaaagtagga aaacagtttc atcccaaaaa aacgaaaaaa cagcaacaaa
    47641 tcccttgcaa tcgcaggggc tttgtgttat tctattatgg tgctgtaaat tacagcctta
    47701 gttttgatgc aagaggttgc gacacgctcg gttgcattgc cacgcaacac cgcgtcggtt
    47761 ttcttgtgga gctagcctat tatcttaaat agacgaaaag gagaaaaaga tggcaaacaa
    47821 aaaaatccgt atccgtttga aagcttacga acaccgtacg cttgacacag cggctgcaaa
    47881 aatcgtagaa tcagctactc gtacaggtgc acaagttgcg ggtccaatcc cacttccaac
    47941 tgaacgtagc ctctacacaa tcattcgtgc gactcacaaa tacaaagact ctcgcgaaca
    48001 atttgaaatg cgtacacaca aacgtttgat cgatatcgtt aacccaactc aaaaaacagt
    48061 tgatgccttg atgaaattgg atcttccaag tggtgtaaac gtagaaatca aactttaatc
    48121 taaaatataa aagagcagag gctggtgttt caatctaatt gaacacgggc taaactcggt
    48181 gtgaaaaaga taaacttcct agtgtctgct agacactgcg tcagtttcct attttgacgt
    48241 tgagtttgac gtcctttgta tcttagactt gagcataaaa aacgctcgtt aaaaactttt
    48301 tgaataaaaa tatagaaaag gaactatttt ctcatgacaa aaggaatctt agggaaaaaa
    48361 gtgggaatga ctcaaatctt cactgaagct ggcgaattga tccctgtaac agttattgaa
    48421 gcaactccaa acgttgttct tcaagttaaa actgttgaaa cagacggata caacgctatc
    48481 caagttggtt tcgatgacaa acgcgaagta ttgagcaaca aacctgctaa aggacatgta
    48541 gcgaaagcta acacggctcc taagcgcttc attcgtgaat tcaaaaacgt tgaaggcttg
    48601 gaagttggcg ctgaaatcac agttgaaaca ttcgcagctg gagatgttgt tgacgtaacg
    48661 ggtacttcta aaggtaaagg tttccaaggt gttatcaaac gccacggaca atcacgtgga
    48721 ccaatggctc acggttctcg ttaccaccgt cgtccaggtt ctatggggcc tgttgcacct
    48781 aaccgcgtat tcaaaggtaa aaaccttgca ggacgtatgg gtggcgaccg cgtaacaatt
    48841 caaaaccttg aagttgtaca agttgttcca gaaaagaacg ttatccttat caaaggtaac
    48901 gtaccaggtg ctaagaaatc tcttatcact atcaaatcag cagttaaagc tggtaaataa
    48961 taaagaaagg ggaaatcagt cacaatggca aacgtaacat tatttgacca aactggtaaa
    49021 gaagctggcc aagttgttct tagcgatgca gtatttggta tcgaaccaaa tgaatcagtt
    49081 gtgtttgatg taatcatcag ccaacgcgca agccttcgtc aaggaacaca cgctgttaaa
    49141 aaccgctctg cagtatcagg tggtggacgc aaaccatggc gtcaaaaagg aactggacgt
    49201 gctcgtcaag gttctatccg ctcaccacaa tggcgtggtg gtggtgttgt cttcggacca
    49261 actccacgtt catacggcta caaacttcca caaaaagttc gtcgcctagc tcttaaatca
    49321 gtttactctg aaaaagttgc tgaaaacaaa ttcgtagctg tagacgctct ttcatttaca
    49381 gctccaaaaa ctgctgaatt tgcaaaagtt cttgcagcat tgagcatcga ttctaaagtt
    49441 cttgttatcc ttgaagaagg aaatgaattc gcagctcttt cagctcgtaa ccttccaaac
    49501 gtgaaagttg caactgctac aactgcaagt gttcttgaca tcgcaaatag cgacaaactt
    49561 cttgtcacac aagcagctat ctctaaaatc gaggaggttc ttgcataatg aatttgtatg
    49621 atgttatcaa aaaacctgtc atcactgaaa gctcaatggc tcaacttgaa gcaggaaaat
    49681 atgtatttga agttgacact cgtgcacaca aacttttgat caagcaagct gttgaagctg
    49741 ctttcgaagg tgttaaagtt gccaatgtta acacaatcaa cgtaaaacca aaagctaaac
    49801 gtgttggacg ttacactggt tttactaaca aaactaaaaa agctatcatc acacttacag
    49861 ctgattcaaa agcaatcgag ttgtttgctg ctgaagctga ataatctaag gaggaaatat
    49921 cgtgggaatt cgtgtttata aaccaacaac aaacggtcgc cgtaatatga cttctttgga
    49981 tttcgctgaa atcacaacaa gcactcctga aaaatcattg cttgttgcat tgaagagcaa
    50041 ggctggtcgt aacaacaacg gtcgtatcac agttcgtcac caaggtggtg gacacaaacg
    50101 tttctaccgt ttggttgact tcaaacgtaa taaagacaac gttgaagcag ttgttaaaac
    50161 aatcgagtac gatccaaacc gttctgcaaa catcgctctt gtacactaca ctgacggtgt
    50221 gaaagcatac atcatcgctc caaaaggtct tgaagtaggt caacgtatcg tttcaggtcc
    50281 agaagcagat atcaaagtcg gaaacgctct tccacttgct aacatcccag ttggtacttt
    50341 gattcacaac atcgagttga aaccaggtcg tggtggtgaa ttggtacgtg ctgctggtgc
    50401 atctgctcaa gtattgggtt ctgaaggtaa atatgttctt gttcgtcttc aatcaggtga
    50461 agttcgtatg attcttggaa cttgccgtgc tacagttggt gttgtcggaa acgaacaaca
    50521 tggacttgta aaccttggta aagcaggacg tagccgttgg aaaggtatcc gcccaacagt
    50581 tcgtggttct gtaatgaacc ctaacgatca cccacacggt ggtggtgaag gtaaagcacc
    50641 agttggtcgt aaagcaccat ctactccatg gggcaaacct gctcttggtc ttaaaactcg
    50701 taacaagaaa gcgaaatctg acaaacttat cgttcgtcgt cgcaacgaga aataatatta
    50761 aactagtcgc ttaagcaact agtaaatccg ccagctcggt agcgctccat aggagtgcaa
    50821 gccgctgtgg tacaacattt aaaggagaaa atataaaaat gggacgcagt cttaaaaaag
    50881 gacctttcgt cgatgagcat ttgatgaaaa aagttgaagc tcaagctaac gacgaaaaga
    50941 aaaaagttat taaaacttgg tcacgtcgtt caacgatctt cccaagtttc attggttaca
    51001 ctattgcagt ttatgacgga cgtaaacacg tacctgttta catccaagaa gacatggtag
    51061 gccacaaact tggtgaattt gcaccaactc gtacttacaa aggtcacgct gcagacgaca
    51121 agaaaacacg tagaaaataa ggagaacata aatggcagaa attacttcag ctaaagcaat
    51181 ggctcgtaca gtacgtgttt cacctcgtaa atcacgtctt gttcttgata acatccgtgg
    51241 taaaagcgta gccgatgcaa tcgcaatctt gacattcact ccaaacaaag ctgctgaaat
    51301 catcttgaaa gttttgaact cagctgtagc taacgctgaa aacaactttg gtttggataa
    51361 agctaacttg gtagtatctg aagcattcgc aaacgaagga ccaactatga aacgtttccg
    51421 tccacgtgcg aaaggttcag cttcaccaat caacaaacgt acagctcaca tcactgtagc
    51481 tgttgcagaa aaataaggag gtaaaatcgt gggtcaaaaa gtacatccaa ttggtatgcg
    51541 tgtcggcatc atccgtgatt gggatgccaa atggtatgct gaaaaagaat acgcggatta
    51601 ccttcatgaa gatcttgcaa tccgtaaatt cgttcaaaaa gaacttgctg acgcagcagt
    51661 ttcaactatt gaaatcgaac gcgcagtaaa caaagttaac gtttcacttc acactgctaa
    51721 accaggtatg gttatcggta aaggtggtgc taacgttgat gcactccgtg caaaacttaa
    51781 caaattgact ggaaaacaag tacacatcaa catcatcgaa atcaaacaac ctgatttgga
    51841 tgctcacctt gtaggtgaag gaattgctcg tcaattggag caacgtgttg ctttccgtcg
    51901 tgcacaaaaa caagcaatcc aacgtgcaat gcgtgctgga gctaaaggaa tcaaaactca
    51961 agtatcaggt cgtttgaacg gtgcagatat cgcccgtgct gaaggatact ctgaaggaac
    52021 tgttccgctt cacacacttc gtgcagatat cgattacgct tgggaagaag cagatactac
    52081 atacggtaaa cttggtgtta aagtatggat ctaccgtggt gaagttcttc cagctcgtaa
    52141 aaacactaaa ggaggtaaat aaccaatgtt agtacctaaa cgtgttaaac accgtcgtga
    52201 gttccgtgga aaaatgcgcg gtgaagcaaa aggtggaaaa gaagtagcat tcggtgaata
    52261 cggtcttcaa gctacaacta gccactggat cactaaccgc caaatcgaag ctgctcgtat
    52321 cgccatgact cgttacatga aacgtggtgg taaagtttgg attaaaatct tcccacacaa
    52381 atcatacact gctaaagcta tcggtgtgcg tatgggatct ggtaaagggg cacctgaagg
    52441 ttgggtagca ccagttaaac gtggtaaagt gatgttcgaa atcgctggtg tatctgaaga
    52501 gattgcacgt gaagcgcttc gacttgctag ccacaaattg ccagttaaat gtaaattcgt
    52561 aaaacgtgaa gcagaataag gagaaggcat gaaacttaat gaagtaaaag aatttgttaa
    52621 agaacttcgt ggtctttctc aagaagaact cgcgaagcgc gaaaacgaat tgaaaaaaga
    52681 attgtttgaa cttcgtttcc aagctgctac tggtcaattg gaacaaacag ctcgcttgaa
    52741 agaagttaaa aaacaaatcg ctcgtatcaa aacagttcaa tctgaagcga aataatagac
    52801 tagggaagga gaaatttcaa tggaacgcaa taatcgtaaa gttcttgttg gacgtgttgt
    52861 atctgacaaa atggacaaga caatcacagt tgtagttgaa acaaaacgta accacccagt
    52921 ctatggtaaa cgtattaact actctaaaaa atacaaagct catgatgaaa acaatgttgc
    52981 caaagaaggc gatatcgtac gtatcatgga aactcgcccg ctttcagcta caaaacgttt
    53041 ccgtcttgta gaagttgttg aagaagcggt catcatctaa tcaaacctga aaggagaaaa
    53101 ctgaaatgat tcaaacagaa actcgtttga aagtcgcaga caacagcggt gctcgcgaaa
    53161 tcttgactat caaagttctt ggtggttcag gacgtaaatt tgcaaacatc ggtgatgtta
    53221 tcgtggcatc tgtaaaacaa gctactcctg gtggtgcggt taaaaaaggt gacgttgtta
    53281 aagcagttat cgttcgtact aaatcaggtg ctcgtcgtgc tgatggttca tacatcaaat
    53341 ttgacgaaaa cgcagcagtt atcatccgtg aagacaaaac tcctcgcgga acacgtatct
    53401 ttggcccagt tgcacgtgaa ttgcgtgaag gtggtttcat gaagatcgtg tcacttgctc
    53461 cagaagtact ttaattttta ggaacaaact agtcccctag cttcaagcta gggtgccctt
    53521 atgggcgtaa gaaaaatcaa ggagaaacct aatgtttgta aaaaaaggcg acaaagttcg
    53581 cgtaatcgct ggtaaagata agggaacaga agctgttgtc cttactgccc ttccaaaagt
    53641 aaacaaagtt atcgttgaag gtgttaacat tgttaagaaa caccaacgtc caactaacga
    53701 gcttcctcaa ggtggtatca tcgagaaaga agcagctatc cacgtatcaa atgttcaagt
    53761 tttggacaaa aatggtgtag ctggtcgtgt tggttacaaa tttgtagacg gtaaaaaagt
    53821 tcgctacaac aaaaaatcag gcgaagtgct tgattaatca cgaaggaaag gagaagtata
    53881 atggcaaatc gtttaaaaga aaaatatctt aatgaagtag ttcctgcttt gacagaacaa
    53941 ttcaactact catcagtgat ggctgtgcct aaagtagata agattgtttt gaacatgggt
    54001 gttggtgaag ctgtatcaaa cgctaaaagc cttgaaaaag ctgctgaaga attggcactt
    54061 atctcaggtc aaaaaccact tatcactaaa gctaaaaaat caatcgccgg cttccgtctt
    54121 cgtgaaggtg ttgcgatcgg tgcaaaagtt acccttcgtg gtgaacgtat gtacgaattc
    54181 ttggataaat tggtatcagt ttcacttcca cgtgtacgtg acttccacgg tgtcccaaca
    54241 aaatcatttg atggacgcgg gaactacaca cttggtgtga aagaacaatt aatcttccca
    54301 gaaatcaact tcgatgacgt tgacaaaact cgtggtcttg acatcgttat cgtaacaact
    54361 gctaacactg acgaagagtc acgtgcattg cttacaggcc ttggaatgcc ttttgcaaaa
    54421 taatatagga ggtaaatcta atggctaaaa aatcaatggt agctagagag gctaaacgcc
    54481 aaaaaattgt tgaccgttat gctgaaaaac gtgctgcatt aaaggcggca ggggactacg
    54541 aaggtttatc taaattacct cgcaacgcct caccgactcg tttacataat cgttgtaggg
    54601 ttacggggcg cccacattca gtttaccgca aatttggtct gagtcgtatc gcttttcgcg
    54661 aacttgcgca taaaggtcaa attcctggtg taacaaaagc atcttggtaa tttaagatat
    54721 caagagcgtc aaaactccaa gtaaaaatag gaaacttgac gaagaaacta aagtttctag
    54781 gaaagtttat ctttttcaca cagagtttag cccgtgttca attgggcttg ccaatttgaa
    54841 cacgagctac agctttggca aaaaagacca atttgctttg gagcattgct tctgcattaa
    54901 attgtctatt tttgctcgtg ctgttacgct ctttgtatca tgtattaact agcaagtgca
    54961 acttgcaaac tactagtaag aggagaaaaa caaaatggtt atgactgacc caatcgcaga
    55021 cttcctaact cgtattcgta atgctaacca agctaaacac gaagtacttg aagtacctgc
    55081 atcaaacatc aaaaaaggga ttgctgaaat ccttaaacgc gaaggttttg taaaaaacgt
    55141 tgaaatcatt gaagatgaca aacaaggcgt catccgtgta tttcttaaat acggaccaaa
    55201 tggtgagaaa gttatcacta acttgaaacg tgtttctaaa ccaggacttc gtgtctacaa
    55261 aaaacgtgaa gaccttccaa aagttcttaa cggacttgga attgccatcc tttcaacttc
    55321 tgaaggtttg cttactgata aagaagcacg ccaaaagaat gttggtggtg aggttatcgc
    55381 ttacgtttgg taaaatcaag atacaaagct cgtaaagaac aaagcaaaat taggaagttg
    55441 gagaagtttg tttacaaaca agccaactta tctattttgc acagttctta gagcgtgttc
    55501 agttcagctc ttgaactaaa taagtatctg aaccccgtga aaactggccg ttctggcctg
    55561 acaatctaac aggagaaaat aaacatgtca cgtattggta ataaagttat cgtgttgcct
    55621 gctggtgttg aactcgctaa caatgacaac gttgtaactg taaaaggacc taaaggagaa
    55681 cttactcgtg agttctcaaa agatattgaa atccgtgtgg aaggtactga aataactctt
    55741 caccgtccaa acgattcaaa agaaatgaaa actatccacg gaactactcg tgcccttttg
    55801 aacaacatgg ttgttggtgt atcagaagga ttcaagaaag aacttgaaat gcgtggggtt
    55861 ggttaccgtg cacagcttca aggatctaaa cttgttttgg ctgttggtaa atctcatcca
    55921 gacgaagttg aagctccaga aggaattact tttgaacttc caaacccaac aacaatcgtt
    55981 gttagcggaa tttcaaaaga agtagttggt caaacagctg cttacgtacg tagccttcgt
    56041 tcaccagaac catataaagg taaaggtatc cgttacgttg gtgaattcgt tcgccgtaaa
    56101 gaaggtaaaa caggtaaata atgttgagtg gttgatcatc aaccaccaac ctattttcca
    56161 actttgtgca tagcacacga tttaaaacta aagaggtgaa aactgtgatt tcaaaaccag
    56221 ataaaaacaa actccgccaa aaacgccacc gtcgcgttcg cggaaaactc tctggaactg
    56281 ctgatcgccc acgtttgaac gtattccgtt ctaatacagg catctacgct caagtgattg
    56341 atgacgtagc gggtgtaacg ctcgcaagtg cttcaactct tgataaagaa gtttcaaaag
    56401 gaactaaaac tgaacaagcc gttgctgtcg gtaaactcgt tgcagaacgt gcaaacgcta
    56461 aaggtatttc agaagtggtg ttcgaccgcg gtggatatct atatcacgga cgtgtgaaag
    56521 ctttggctga tgcagctcgt gaaaacggat tgaaattcta ataggaggac actagaaaat
    56581 ggcatttaaa gacaatgcag ttgaattaga agaacgcgta gttgctgtca accgtgttac
    56641 aaaagttgtt aaaggtggac gtcgtcttcg tttcgcagct cttgttgttg ttggtgacca
    56701 caatggtcgc gtaggatttg gtactggtaa agctcaagaa gttccagaag caatccgtaa
    56761 agcagtagat gatgctaaga aaaacttgat cgaagttcct atggttggaa caacaatccc
    56821 acacgaagtt ctttcagaat tcggtggagc taaagtattg ttgaaacctg ctgtagaagg
    56881 ttctggagtt gccgctggtg gtgcagttcg tgccgttgtg gaattggcag gtgtggcaga
    56941 tattacatct aaatcacttg gttctaacac tccaatcaac attgttcgtg caactgttga
    57001 aggtttgaaa caattgaaac gcgctgaaga aattgctgcc cttcgtggta tttcagtttc
    57061 tgatttggca taagaaaggg gataaaatgg ctcaaattaa aattactttg actaagtctc
    57121 caatcggacg cattccatca caacgtaaaa ctgttgtagc acttggactt ggcaaattga
    57181 acagctctgt tattaaagaa gataacgctg ctatccgtgg tatgatcaca gcagtatctc
    57241 acttagtaac agttgaagaa gtaaactaat gaagttttag gggatgtgca ctgtaccatc
    57301 ccctaaaact agatatagtc atctatgatg acatcgtata ggcgagttga tgggggagac
    57361 aaccttttct cccttatcgg cgctagcatt ttacaaaaga ggagaaaata aaaatgaaac
    57421 ttcatgaatt gaaacctgca gaaggttctc gtaaagtacg taaccgcgtt ggtcgtggta
    57481 cttcatcagg taacggtaaa acatctggtc gtggtcaaaa aggtcaaaaa gctcgtagcg
    57541 gtggcggagt tcgccttggt tttgaaggtg gacaaactcc attgttccgt cgtcttccaa
    57601 aacgtggatt cactaacatc aacgctaaag aatacgcaat tgtgaacctt gaccaattga
    57661 acgtctttga agatggtgct gaagtaactc cagttgttct tatcgaagca ggaattgtta
    57721 aagctgaaaa gtcaggtatt aaaattcttg gtaacggtga gttgactaag aaattgactg
    57781 tgaaagcagc taaattctct aaatcagctg aagaagctat cactgctaaa ggtggttcag
    57841 tagaagtcat ctaagagagg tgacctatgt tttttaaatt attaagagaa gctcttaaag
    57901 tcaagcaggt tcgatcaaaa attttattta caatttttat cgttttggtc tttcgtatcg
    57961 gaactagcat tacagttcct ggtgtgaatg ccaatagctt gaatgcttta agtggattat
    58021 ccttcttaaa catgttgagc ttggtgtcgg ggaatgccct aaaaaacttt tcgatttttg
    58081 ccctaggagt tagtccctat atcaccgctt ctattgttgt ccaactcttg caaatggata
    58141 ttttacccaa gtttgtagag tggggtaaac aaggggaagt aggtcgaaga aaattgaatc
    58201 aagctactcg ttatattgct ctagttctcg cttttgtgca atctatcggg attacagctg
    58261 gttttaatac cttggctgga gctcaattga ttaaaactgc tttaactcca caagtttttc
    58321 tgacgattgg tatcatctta acagctggta gtatgattgt cacttggttg ggtgagcaaa
    58381 ttacagataa gggatacgga aacggtgttt ccatgattat ctttgccggg attgtttcct
    58441 caattccaga gatgattcag ggcatctatg tggactactt tgtgaacgtc ccaagtagcc
    58501 gtatcacttc atctatcatt ttcgtaatca ttttgattat tactgtattg ttgattattt
    58561 actttacaac ttatgttcaa caagcagaat acaaaattcc aatccaatat actaaggttg
    58621 cacaaggtgc tccatctagc tcttaccttc cgttaaaggt aaatcctgct ggagttatcc
    58681 ctgttatctt tgccagttcg attactgcag cgcctgcggc tattcttcag tttttgagtg
    58741 ccacaggtca tgattgggct tgggtaaggg tagcacaaga gatgttggca actacttctc
    58801 caactggtat tgccatgtat gctttgttga ttattctctt tacattcttc tatacgtttg
    58861 tacagattaa tcctgaaaaa gcagcagaga gcctacaaaa gagtggtgcc tatatccatg
    58921 gagttcgtcc tggtaaaggt acagaagaat atatgtctaa acttcttcgt cgtcttgcaa
    58981 ctgttggttc cctcttcctt ggtgtgattt ccattttacc gattgcagct aaagatgtat
    59041 ttggtctttc tgatgttgtt gcctttggtg gaacaagtct cttgatcatt atctctacag
    59101 gtatcgaagg aatcaagcaa ttggaaggtt acctattgaa acgtaagtat gttggtttca
    59161 tggacagaac agaataaaag tatttactga atcagtaaat gctgagggag tggaggttta
    59221 aactctgaca tttgtaagag ttggatctcc cctcttctat tttgttttta aataggggtg
    59281 aaaagacttt ttgcttctat ttaaaaataa aataaggaga tcaaatcatg aatcttttga
    59341 ttatgggctt acctggtgca ggtaagggaa ctcaagcagc aaaaatcgta gaacaattcc
    59401 atgttgcaca tatctcaaca ggtgatatgt tccgcgctgc aatggcaaat caaactgaaa
    59461 tgggtgttct tgctaagtca tatattgaca agggtgaatt ggttcctgac gaagttacaa
    59521 atggaatcgt aaaagaacgt ctttcacaag atgatattaa agaaacagga ttcttattgg
    59581 atggttaccc acgtacaatt gaacaagctc atgccttgga caaaacattg gctgaacttg
    59641 gcattgaact agaaggtgtt atcaatattg aagtgaaccc tgacagcctc ttggaacgtt
    59701 tgagtggccg tatcatccac cgcgtaactg gagaaacttt ccacaaggtc tttaacccac
    59761 cagttgacta taaagaagaa gattattacc aacgtgaaga tgataagcct gagacagtaa
    59821 aacgtcgttt ggatgttaat attgctcaag gagaaccaat cattgctcac taccgtgcca
    59881 aaggtttggt tcatgacatc gaaggtaatc aagatatcaa tgatgtcttc tcagatattg
    59941 aaaaagtatt gacaaatttg aaataaagcg tttttcatac ttgcaaaaat tcgctacaaa
    60001 tgttatactg aaatagtctg acttataatt gttgtctctg tgtctagagg catcgaatcg
    60061 aaatttatgg aggtgctttt gcgtggcaaa agacgatgtg attgaagttg aaggcaaagt
    60121 agttgataca atgccgaatg caatgtttac ggttgaactt gaaaatggac atcagatttt
    60181 agcaacagtt tctggtaaaa ttcgtaaaaa ctatattcgt attttagcgg gagatcgtgt
    60241 tactgtcgaa atgagtccat atgacttgac acgtggacgt atcacttacc gctttaaata
    60301 atcgaaaaac ttggagggat aagaaatgaa agtaagacca tcggtcaaac caatttgcga
    60361 atactgtaaa gttattcgtc gtaatggtcg tgttatggta atttgcccag caaatccaaa
    60421 acacaaacaa cgtcaaggat aagatagaaa ggagaaaaca tggctcgtat tgctggagtt
    60481 gatattccaa atgacaaacg cgtagtaatc tcattgactt atgtttatgg tatcggactt
    60541 gcaacatcta agaaaatttt ggctgctgct ggaatctcag aagatgttcg tgtacgtgat
    60601 cttacatcag atcaagaaga tgctatccgt cgtgaagtgg atgcaatcaa agttgaaggt
    60661 gaccttcgtc gtgaagtaaa cttgaacatc aaacgtttga tggaaatcgg ttcataccgt
    60721 ggtatccgtc accgtcgtgg acttcctgtc cgtggacaaa atactaaaaa caacgctcgc
    60781 actcgtaaag gtaaagctgt tgcgattgct ggtaagaaaa aataatatag gaggtaaaag
    60841 tcttggctaa accaacacgt aaacgtcgtg tgaaaaagaa tatcgaatct ggtattgctc
    60901 atattcacgc tacatttaat aacactattg ttatgattac tgatgtgcat ggtaatgcaa
    60961 ttgcttggtc atcagctggt gctcttggtt tcaaaggttc tcgtaaatct acaccattcg
    61021 ctgctcaaat ggcttctgaa gctgctgcta aatctgcaca agaacacggt cttaaatcag
    61081 ttgaagttac tgtaaaaggt ccaggttctg gtcgtgagtc agctattcgt gcgcttgctg
    61141 ccgctggtct tgaagtaaca gcaattcgtg atgtgactcc agtgccacac aatggtgctc
    61201 gtcctccaaa acgtcgccgt gtataatcat cgcattacac tgcttttcgt ttaagaggga
    61261 gtaactaaat gatcgagttt gaaaaaccaa atataacaaa aattgatgaa aataaagatt
    61321 atggcaagtt tgtaatcgaa ccacttgaac gtggctacgg tacaactctt ggtaactctc
    61381 ttcgtcgtgt acttctagct tctctaccag gagcagctgt gacatctatc aacattgatg
    61441 gtgtgttaca tgagtttgac acagttccag gtgttcgtga agacgtgatg caaatcattc
    61501 tgaacattaa aggaattgca gtgaaatcgt acgttgaaga cgaaaaaatc atcgaactgg
    61561 atgttgaagg tcctgctgaa gtaacagctg gtgacatttt gacagatagc gatattgaaa
    61621 ttgtaaatcc agatcattat ctctttacaa ttggtgaagg ttcttctcta aaagcgacta
    61681 tgactgttaa cagtggtcgt ggatatgtac ctgctgatga aaataaaaag gataatgcac
    61741 cagttggaac acttgctgta gattctattt atacaccagt tacaaaagtc aactatcaag
    61801 tggaacctgc tcgtgtaggt agcaatgatg gtttcgacaa attaaccctt gaaatcttga
    61861 caaatggaac aattattcca gaagatgctt tagggctttc agcacgtatt ttgacagaac
    61921 atcttgattt gtttacaaat cttactgaga ttgctaagtc aactgaagtg atgaaagaag
    61981 ctgatactga atctgacgac cgtattttag atcgtacgat tgaggaactg gacttgtctg
    62041 tgcgttcata caactgttta aaacgtgccg gtatcaatac tgtgcatgat ttgacagaaa
    62101 aatctgaagc agagatgatg aaagtacgaa atcttggacg caagagtttg gaagaagtga
    62161 aactcaaact cattgatttg ggtcttggat taaaagataa ataaaggagg aatacatggc
    62221 ttaccgtaaa ctaggacgca ctagctcaca acgtaaagca atgcttcgcg atttgacaac
    62281 tgaccttttg atcaacgaat caatcgtgac aactgaagct cgtgctaaag aaatccgtaa
    62341 aactgttgaa aaaatgatta ctctaggtaa acgtggtgat ttgcatgcac gtcgtcaagc
    62401 agctgctttc gtacgtaatg aaatcgcatc tgaaaactat gatgaagcaa ctgataagta
    62461 cacttctact acagcacttc aaaaattgtt ctcagaaatc gcacctcgtt atgctgaacg
    62521 taacggtgga tacactcgta tccttaaaac tgaaccacgt cgtggtgatg cagcgccaat
    62581 ggcgatcatc gaattagtat aaaatcatca attttgttga gtgttatgat gatggagtct
    62641 tgtgctctta gtctagctct ggtctaccgc taggatttcg gtcctagcgg gaacactcat
    62701 cataagttgg gatagtagac gcttgtttac gaaattgttt ttttcttaag aacaacttcg
    62761 taagcaggcg tttttgagta ttttcgttag aattatgcta tactatttga aaagaatcct
    62821 gtttaatgtt aaggtttctt attttaagaa gaattggagt ttacttatga aagccattat
    62881 aactgttgtt ggtaaagata aatctggaat tgttgcaggt gtttctggta aaattgcaga
    62941 attaggattg aatattgacg atatctctca aactgtcttg gatgaatatt ttacgatgat
    63001 ggctgttgta tctagtgatg aaaagcaaga ttttacctat cttcgtaatg aatttgaagc
    63061 ttttgggcaa actttgaatg taaaaatcaa tattcagagt gcagcgattt tcgaagctat
    63121 gtataatatc taggaggtca tcatggatat tagacaagtt actgaaacca tcgccatgat
    63181 tgaggagcaa aacttcgata ttagaaccat taccatgggg atttctcttt tggactgtat
    63241 cgatccagat atcaatcgtg ctgcggagaa aatctatcaa aaaattacga caaaggcggc
    63301 taatttagta gctgttggtg atgaaattgc ggctgagttg ggaattccta tcgttaataa
    63361 gcgtgtatcg gtgacaccta tttctctgat tggggcagcg acagatgcga cggactacgt
    63421 ggttctggca aaagcgcttg ataaggctgc gaaagagatt ggtgtggact ttattggtgg
    63481 tttttctgcc ttagtacaaa aaggttatca aaagggagat gagattctca tcaattccat
    63541 tcctcgcgct ttgactgaga cggataaggt ctgctcgtca gtcaatatcg gctcaaccaa
    63601 gtctggtatt aatatgacgg ctgtggcaga tatgggacga attatcaagg aaacggcaaa
    63661 tctttcagat atgggagcgg ctaagttggt tgtattcgct aatgctgttg aggacaatcc
    63721 atttatggcg ggtgcctttc atggtgttgg ggaagcagat gttatcatca atgtcggagt
    63781 ttctggtcct ggtgtggtga aacgtgcttt ggaaaaagtt cgtggacaga gctttgatgt
    63841 agtagccgaa acagttaaga aaactgcctt taaaatcact cgtatcggtc aattggttgg
    63901 tcaaatggcc agtgagagac tgggtgtgga gtttggtatt gtggacttga gtttggcacc
    63961 aacccctgcg attggagact ctgtggcacg tgtccttgag gaaatggggc tagaaacagt
    64021 tggcacgcat ggaacgacgg ttgccttggc cctcttgaac gaccaagtta aaaagggtgg
    64081 agtgatggcc tgcaaccaag tcggtggttt atctggcgcc tttatccctg tttctgagga
    64141 tgaaggaatg attgctgcag tgcaaaatgg ctctcttaat ttagaaaaac tagaagctat
    64201 gacggctatc tgttctgttg gattggatat gattgccatc ccagaagata cgcctgctga
    64261 aaccattgcg gctatgattg cggatgaagc agcaatcggt gttatcaaca tgaaaacaac
    64321 agctgttcgt atcattccca aaggaagaga aggcgatatg attgagtttg gtggtctatt
    64381 aggaactgca cctgttatga aggttaatgg ggcttcgtct gtcgacttca tctctcgcgg
    64441 tggacaaatc ccagcaccaa ttcatagttt taaaaattaa gaaaatagga gaaattttaa
    64501 gttctattta agattagatg tgtatactat aatcattaaa taaagacctc ctaatattat
    64561 ttgaaacaga taacactgaa ttagtttgaa tttgattttc atctaatatc tttatttaat
    64621 gaactcctaa actttttcat aataatctcc ttcaaaagtc gcctgtatgg gtggctttta
    64681 ttttatcatt catgatataa tagaagcaaa cggaggacgg aaaatggtaa aagtacgatt
    64741 gtatttggta cgtcatggca agaccatgtt taacacgatt ggtcgcgcgc aaggttggag
    64801 cgatactccc ttaactgctg aaggtgaacg agggattcaa gagttaggaa tcggtttgcg
    64861 agaatctgat ctacagtttg agcgcgctta ttcgagtgat tctggtcgta ccattcagac
    64921 catgggaatt atccttgaag aacttggctt gcaggggaaa atcccttatc gcatggacaa
    64981 gcgtatcaga gaatggtgtt tcggtagttt tgatggagcc tatgatggcg atcttttcat
    65041 gggcattatt cctcgtatct ttaatgtgga ccacgttcac caattgtctt atgctgaact
    65101 ggctgagggc ttggtagagg tcgatacagc tggttgggct gaaggctggg aaaaactcag
    65161 tggccgaatc aaggaaggct ttgaaatgat tgcaaaagaa atggaagatc aaggtggagg
    65221 taacgccctt gttgtcagcc atggaatgac tattggaacc attgtttatc tgattaatgg
    65281 catgcatccg catggtctag ataatggtag cgtgacgatt cttgaatatg aggacggtca
    65341 gtttagggtt gaagttgtcg gtgaccgtag ttaccgagag ctaggatgtg agaagatgga
    65401 agaaggctct atttaatcag tctagacttg cttgccatga gctagggatt tgataagaat
    65461 atcaagataa gaaaaaacag ccgagggcac tcctttcggc tgtttttgat gtggaaaact
    65521 aaagtgtaat gctattgctt ttagagattt tcataaacaa gagcaaggaa cctactgtta
    65581 gaacagtcag gatagttgac aaggctgcgg ctacaccgta atttcctctg agaacctctg
    65641 tataaatagc tacagtcatt gttcttgttt tgacattgta gaggaggata gaagtagaga
    65701 gttttgaaat cattgtgacc caagataaga tggctccaga aatgatacca gatagcatca
    65761 ttggagttgt aatcttagca aaggtattga gacgactact tcctaagctt tcagcagctt
    65821 cttcaatact tggtgctatt tgttgtaagc tagcaacaga tgagcgaata gtataaggta
    65881 atcttctgac agatagagac ataatcaaga tgaaagcagt ccctgtaatc ataagaaatc
    65941 cacttccaaa tagaccagta ttgaaggaag aaatgaaggc aatccctaga acggttcctg
    66001 gtacaatata aggtaccata ctgaggctgt caattaagtt tgtaaacaaa ttccgttttc
    66061 taacggctag gtaggagata aatgtcgcaa atagaacaac tagaactaag gcaatcaaag
    66121 ggatacgaat ggtattgaaa atagcagatc ccatacgatt gaaagctacc ttgtaactgt
    66181 ttggagaata acctttaaca gataccatac ctgatgtttt taggaaagag gtataaatta
    66241 agtagatttg aggtaaaaca gagataaaga taattccgta gactgttgca taaatggcag
    66301 ctatttttcc ttttgtagtt tttttaggct caattggatg gagcagattc atgctgaaac
    66361 tgtagcggtt tgcaatgtgt ttttggataa ggaaaattgc caaggcaatg ataatcgcca
    66421 taattgcaaa agcagaattt cctccaacct cgctaataaa ttgggtataa atcaggacag
    66481 ggaaagtccg atatccttcg tcaatcaaca taggcgttcc aaagtctgag aatgctctca
    66541 taaatacaag caaggagctg ctagtaaggt tggaactagg agaggtaaaa caaccgttac
    66601 gatacgctta aatccgaagg accccatgct ttcagctgct tcaagtagag aattgtcaat
    66661 actgttcatt gttccagcaa catatagaaa taccagtggg aatagttgca gtgtaaagac
    66721 aagtacaatt cctttgaatc cataaatatc gatagctgga agatgaaggg catttgtcaa
    66781 aaatttagtg atgacctcat ttcgtcccag caagagaatt caggagtagg ctcctacgaa
    66841 aggagctgac atggaagcaa tgataatcaa tatttgtaga aatttcttcc ccttgaagtc
    66901 atacatagag aagagataag ctaatagggt tcctacaact aaggaagtga tagtagcggt
    66961 aatggaaacc ttgaaactgt tgactagtgt ctcagagtag taggctttac taaagaaagt
    67021 gacaaaatta gctagtgaga attgtccttc atgtataagt gcttgcttga gcacggtaac
    67081 gataggataa acgagaaaga caagataggt aagaaagatg aagaaagagg aagctgtcca
    67141 aatatttagt tttttacatt ccatggttga ctccttttat caggttttgg gaaccatctg
    67201 cagaaaagat gtttaatttt tgcgtattga ttcgtagacg aatacgattg cctttttgta
    67261 gttcttcttc aaaagttgat tcttcactaa cttaaatttt tgaggcaaaa cctgtctcaa
    67321 tgaaatactc cgtatttagt ccaagataga cgctatcgct aatagttcct tcgatatctc
    67381 cagattcatc tttgataaac tcttcgggac gaatgcttac atgaatagct tgctcctcaa
    67441 cctgatcaag agctggcatt cgaagggcat agccatctga aaagacgata taagcgccgt
    67501 cgctccgttt ttcaagattg gcagggataa tatttgtgcg tccgataaag gttgccacaa
    67561 actcattagc tggtttatga tagagttatt ttggtcggcc gatttgttgg atcaccccat
    67621 ctttcataac agcaatttgg tctgaaatag ccatggcttc ttcttggtcg tgggttacat
    67681 aaacagttgt aattcccact tcgtgttgga tttctcggat ggcttgacgc atatccaagc
    67741 gaagtttggc ctccagatta ctaagtggct cgtccatgag gagaacactt ggattaaccg
    67801 ctaaggcgca tgccaaggtg acacgttgtt gttgtccacc actgagttta tcgggctttc
    67861 gatccgcata ttgagcaatt tgcatgagtt caagatactt gttggtctgt tgaatcaatt
    67921 cttcttttgg aaccttcttt tgcataagac caaaagcaac gttgtctcgg acagtcaaat
    67981 gtgggaaaat agcgtagttt tggaaaacca tcccgatatt gcgtttgctg ggttccatat
    68041 tattgatttt tgtatcatcg aagtaaaatt ctccaccttc gatactgttg aaacctgcaa
    68101 tcatacgaag aagggtcgtt ttcccacatc ctgaagctcc aagaagggta aagagacttc
    68161 cttttggaat tgtaatgttc aaattctcaa taacagggac atcgtggtag atttttttgg
    68221 cgttaataat ttcgatctca ctcatagtga acctctttta ctgtttagat tggatatctg
    68281 taaagacttc gttgtatttc ttaacgatat ctgatttatt cttgatgaca taatcataat
    68341 cttcagtgag tgttttgatt ttgtcaattg gtttcatgtt ttcgcttgtt ttagcatttt
    68401 tacgaacagg acggttagta gtggttgtac caagtgtatc ttgtacttct tgagagataa
    68461 taaaatcgat aaatttcttg gcattttcca tatttttaga ttttttaacg atagcagcac
    68521 tagcaggtag gaagacggtt ccttcttttg gatagactac cttaatgtta gctccgtcat
    68581 ttaagagttt aactgctgga tcttcataag agagactaac agtcatttct ccatcagcga
    68641 ctgctttata gacaccagat gaacttaaac cgattttata gtatattgaa actagaatag
    68701 tacacatcta cttctaaaac attgttagaa atcgatttga ctgtcctgat taatttgtcc
    68761 tgttcttatt tcattttact ataccatcaa taagtgtgaa aagatctttt acataagacc
    68821 aagccttatc atctttgtaa ccaccttgag cttgtagcat atttgttaat tgagcaaagg
    68881 cgctagaaga gtttgctggg tcagcagttg cgatttttcc ttttagttca ggtttgagaa
    68941 ggtcgctata tccttcgatg ttcatgcctt tagttaaatc agggtggacg attaaaacac
    69001 taccatctag tgtataagga gtagagaagc cagttgtgtt ttgatattct ttgataacat
    69061 tatcattttc ttttgaaata tagttttcaa agagttctcc gtgggtagca tattgtgtat
    69121 aagaaccacc aaagataata tcagctacag gaacttcttt ttctgactct agttttttga
    69181 aaagttctcc agtaccagct tgaatcagtt ctattttgat accatatttt tcttcaaagg
    69241 caggaatagt tgctccaatt aagccttctg agtttggtga ataaacgact agcgaaccac
    69301 cgtctccttt atcagatgaa ctgtcatcgg cagattcatt agaagaacaa gcagcataat
    69361 acatccattt ctttttcatg atggatacct ccgttgtgtt atttaagttt attttaaaac
    69421 aatgtaagcg tttttaaaac ctacaattct attctatagt gtattgaatc tataacagta
    69481 cacattgact gctaaaatat ttctataaat taatttgact ttcctgatag agatgttcac
    69541 atcttatttc aattcactat attagagtaa aattctctac aaaaaaaaga atagcctatt
    69601 ttactatttt tccgagtgat ttcaattcct ttggggaaat atggagatac tttttaaatc
    69661 ctgacaaatg gttgtttctt tttctaaatc ggtgatactg tatcggagaa tgcgcgtgag
    69721 gtcacaaagg ctgcgataga gcttctatgg agaatttctt tttggagaga ttttttaaag
    69781 gaatgagaca tccgctacct ccttggaagg tttttttgtg ataagtatag cagtttaaaa
    69841 attctagagc aaggtaaaat agaaaaggtt tggctaaaat tttttagcta aaccttttga
    69901 aaagttaagt atgtattata gatgcttgtt atctgcaaat agctagagca tttcaaggaa
    69961 ataaactaga aataacaatt aatatggtgg ttcagaaata ccttttgata atcaataaga
    70021 tcttcaggat gaagtgcgtt gattccatcc atttcatatt tccgatttaa taaacgatat
    70081 ttgttttcac caaattgatg aaaagggagt agttgaactt ggtcgatatt taatgagtta
    70141 aatagagtag cgaatttttc tgcatcctct aaactattgt taaaattagg aataactggg
    70201 attcttaaaa cgatagtttt attttgtgaa aaagcataat gaatgttttt aataatcatt
    70261 tgattaaaaa ccccagtcac ttttttatgt tttatagaat tataatgttt taggtctgtg
    70321 tagataaaat ccacatattg aattaaatca ataaattttt catgatcaac aaaggcagta
    70381 gtttcaatgg cagtgtgtat gtgatgttct ttagctgatt ttaagatagc tttagcaaat
    70441 tcaaactgag caaatatttc acctcctgat aaagttaaac ctccaccgga ttcttcgtaa
    70501 aattctttgt cttttaatac ctctgtaata atttcttcta cagtcttttc ttcaccgact
    70561 aaggtgaatt tctctcgttg agcatctttc attttttcag gtttcattct ttgagattca
    70621 ggattagaac accatggaca gcgcagagga catcctttta aaaaaacagt tgtacgaata
    70681 cccgggccgt catgaattga aaagtgttga atattaaaaa taattccttt agatatttcc
    70741 ataagagttc ctcctcttca taagtctatc ctatcacaaa aacgaaagaa aaacaattac
    70801 gaacgaaagg ttaatttgtt gttttttgct ttttattttg gtaggataaa acggaggtgt
    70861 aaaatgaaaa gattggaaca aattattaaa ttagtatcag aatatgaaaa gatcgacgtt
    70921 aatacattat cggaaaaatt aaatgtatcg aaagtaacga ttagaaaaga tttagataaa
    70981 ttagagtcaa aaggtttatt acacagagag catggatatg ctgtattaaa tagtggagat
    71041 gacttaaatg tacgtttgtc aattaattat gaaattaaga gaaaaattgt tcaggaagca
    71101 gtaaaattgg tgtcagataa tgaaacgata atgatagaat ctggatcgac ctgtgcttta
    71161 cttgctgagg aaatttgcaa gcaaaaaaga aatgttacga ttgtaacaaa ttcatttttt
    71221 atagcaaatt ttgtgagagc ttatgattca tgtcgtatta ttgttcttgg tggagagttt
    71281 cagaaggatt cacaggttac tgtgggacct ttattaaaag aaatgataca gacttttcat
    71341 gtgcatcaag cttttgttgg gacagatgga tacgataaag agatgggctt tacaggaaaa
    71401 gatttaatgc gcagtgaggt agttcaatat atttcagcag cgtcggataa agtcattgtc
    71461 ctaactgact caagtaaatt tgataaaagg ggtacagtaa gaagatttgc tttaagtcaa
    71521 gtctatgaag taataacaga cgaaaaactt tctaaacaaa atatagctac attagaaaat
    71581 gctgggataa tggttaaggt agtttcgtaa gaggttaagt gtatgaatca agataggaat
    71641 aaactgctct ctaaaattgc ttatctgtat tatattgaaa acttaaatca gtcacaaata
    71701 gcagcaaaat taggaattta tagaacctct attagtagaa tgttaacaga agcaaggaat
    71761 gcaggaattg ttaaaattga aatagagaat tttgatacca atatgtttaa gttggaaaat
    71821 tatgtaaaag aaaaatatgg tctggaaagt ttagaaatta ttccaaatga atttgatgat
    71881 aatccaacaa ttttatctga aagaatttct caagttgcag caggtgtcct taggaatcta
    71941 attgatgata atatgaaaat tggcttttct tgggggaaaa gtttaagtaa tttagtagat
    72001 ttaattcaca gtaaaagtgt ccgaaatgtt cacttctatc ctctagcagg tggtcctagt
    72061 cacatacacg ctaaatacca tgtgaataca ctgatttatg aaatgtctag aaaatttcat
    72121 ggagagtgta catttatgaa tgcaacgatt gtgcaagaaa ataaattgtt agcagatggt
    72181 attttgcaat caagatattt tgaaaatttg aaaaatagtt ggaaagattt agatatagct
    72241 gtagtcggaa ttggtgattt tagcaataaa ggaaagcatc aatggttaga catgcttaca
    72301 gaggatgact ttaaagaatt gaccaaagtt aaaactgtag gagaaatttg ttgtcgattt
    72361 tttgattcaa aaggtaaaga agtctatgaa aacttacagg aaagaacgat agcaatctct
    72421 ttggaagatt taaaaaatat tcctcaaagt ttagctgttg cttacggtga tacgaaagta
    72481 tcttcgattc tttctgtctt gcgtgctaat ttagtaaatc atttgattac agacaaaaat
    72541 acaattttaa aagttttgga agaagatggg gatttgactt ttagagagat tctaggtgag
    72601 tgaaaatgat agactgattc agtttatcgt ttttcttttt agttgattgc acatttgtgc
    72661 ttatataaac aaaaatagtt tatctgttgt ttttggattg acaactttat tatgtagttg
    72721 tattctatag ttacaaaaga aaattttaaa atttcaaatg aaaaaagctt tttacatagt
    72781 gaaatgagga ggaatttatg gaaattattg ttccagatca aattatcatg ggtttaattt
    72841 tatatgctgg tgatgcgaaa caacatattt ataaagcgtt agattacata aaaaatggta
    72901 catgtgaacg gtgtgaagaa gaaatccagt tagctgatgc cgccttatta gaagcccata
    72961 atctacaaac aaaattcttg gcacaagaag cgtctggtac aaagacagaa attacagctc
    73021 tctttgttca ttcacaagat catctcatga ctagtatgac ggagattaat ttaatcaaag
    73081 aaattattag tttgagaaaa gaacttcata aaaaataata ctagagtatt atcattgtta
    73141 ttaacataga ggaggaaaac ataatggtga agattggttt gttttgtgca gcaggttttt
    73201 ctactggtat gcttgtaaat aatatgaaaa ttgcagcgca atctagtgga gttgaggcag
    73261 aaatagaggc gttttctcag tctaaattag cggattatgc gccaaatata gatgttgcac
    73321 tattgggtcc acaagttgct tatacattag ataaatcaaa agaaatttgt gataagtgtg
    73381 atgttccgat agctgttatt ccgatgatgg actatggtat gttagatggg aaaaaagtat
    73441 tagatttggc cctatctttg attagtgggt aagaaaagga gatttattat gtcaaagatg
    73501 gatgttcaga aaatcattgc accgatgatg aagtttgtga atatgcgtgg cattatagct
    73561 ctaaaagatg ggatgttagc aattttgcca ttgacagtag ttggtagttt gttcttgatt
    73621 atgggacaat tgccgttcga aggattaaat aagagcattg ctagtgtttt tggagctaat
    73681 tggacagagc cgtttatgca agtatattca ggaacttttg ctattatggg tctaatttct
    73741 tgtttttcaa ttgcctattc ttatgctaag aatagcggag tagaggcttt accagctgga
    73801 gttctatctg tatctgcatt ctttattttg ctaagatcat cttatatccc taaacaaggt
    73861 gaggcgattg gggacgctat tagtaaagtt tggtttggag gccaaggaat tatcggtgct
    73921 atcattatag gtttggtagt aggaagtatt tataccttct ttataaagag aaagattgtt
    73981 attaagatgc cagaacaagt tccacaagct attgccaaac agtttgaagc aatgattcca
    74041 gcatttgtaa ttttcttatc ttctatgatt gtatatattt tagcgaagtc attgactaat
    74101 ggcggaacat tcatagaaat gatttattct gctattcaag ttccgttgca aggtttaact
    74161 ggatctttgt atggtgctat tggaattgca ttctttatat catttttgtg gtggtttggt
    74221 gttcatgggc aatcggtagt aaatggagta gtgacagctc ttcttttatc taatcttgat
    74281 gctaataaag ctatgttagc ctctgctaat ctatcattag aaaatggagc acatattgtt
    74341 actcaacagt ttttagattc atttttaatt ctatcaggtt cagggattac gtttggtctt
    74401 gtagttgcca tgctttttgc agcaaaatca aaacaatacc aagccttagg aaaagttgca
    74461 gcttttccag caatatttaa cgtaaatgag ccagttgtat ttggatttcc gattgtcatg
    74521 aatccagtta tgtttgtacc tttcattctt gttcctgtac ttgcagctgt gatagtatat
    74581 ggagctattg caacaggttt catgcagcca ttctcagggg taacattgcc ttggagtaca
    74641 ccagctattc tatcaggatt tttggtgggt ggatggcaag gtgttgtgac tcagttatta
    74701 gtattggtga tatctacagt ggtttacttt ccattcttta agatacaaga tcgtttagct
    74761 taccaaaatg aaatcaaaca atcttagagg tatttgtgtg ttactgttaa actcacacat
    74821 ttgtgctaaa aattagagag ttaaaatttt tctagttaaa agcttgaaaa tttctataaa
    74881 aatcggtatt atattttcga aagaaataaa aatattttcg aaagaaaggt gcttacgatg
    74941 gtaaatacag aagtagcaag aacaacaatc aagacagaat attttggcag ccttactgaa
    75001 aggatgaaca aatatcgaga agatgtttta aataaaaaac cttatattga tgctgagaga
    75061 gcagttctag caacacgcgc ctatgaacga tacaaggaac aacctaatgt cctaaaacgt
    75121 gcatatatgc tgaaagaaat tttggaaaat atgtctatct atattgaaga agaatctatg
    75181 attgcgggaa atcaagcttc ttccaataaa gatgctccta tttttccgga atatacgcta
    75241 gaatttgttc tcaatgagtt ggatcttttt gaaaagcgtg atggagatgt tttctatatt
    75301 acagaagaaa caaaagaaca acttagaagt attgctccgt tttgggaaaa taataattta
    75361 cgtgctagag ctggtgcctt attacctgaa gaagtgtctg tttatatgga aacaggattc
    75421 ttcggtatgg aaggtaagat gaattctgga gatgctcact tagcagttaa ctatcagaaa
    75481 cttttgcaat ttggtttaag aggttttgaa gagcgggctc gtaaagcaaa agtagctcta
    75541 gatttaacag atccagcaag tattgataaa tatcattttt acgactctat atttatcgta
    75601 atcgatgcta ttaaagtata tgcaaagcgc tttgttgctc ttgctaaaag tttagccgaa
    75661 aatgcaaatc ctaaacgtaa gaaagaatta cttgagattg cagatatttg ctctagagtc
    75721 ccatatgaac cggcaactac ttttgcagaa gctattcaat cagtttggtt tattcaatgt
    75781 attttacaaa ttgaatctaa tggccactct ctttcatatg gccgttttga tcaatatatg
    75841 tatccatata tgaaggctga tttagaaagt ggtaaagaaa cagaagatag cattgttgaa
    75901 cgtctgacaa atctttggat taagacaatt acaattaata aggttcgcag tcaatcacat
    75961 acattttctt cagcaggaag tcctttatat caaaatgtta caattggtgg acagactcga
    76021 gataagaagg atgctgttaa cccattatct tatttggtct taaaatcagt tgcacaaacc
    76081 catctaccgc aacctaatct aactgtacgt taccatgcag gtttagatgc tcgtttcatg
    76141 aatgagtgta ttgaagtgat gaaacttggt tttggtatgc ctgcatttaa taatgatgag
    76201 attattattc cttcttttat tgcaaaagga gtattggaag atgatgctta tgattacagt
    76261 gccattggat gtgttgaaac ggcagttcca gggaaatggg gctatcgttg cacaggtatg
    76321 agttatatga acttccctaa ggttctactt atcacgatga atgatggaat tgatccggct
    76381 tcgggtaaac ggtttgcacc aagctttggt cattttaagg atatgaagaa cttttctgaa
    76441 ttagaaaatg cttgggataa aacactaaga tatttgacac gaatgagtgt tattgttgaa
    76501 aattctattg atttatcatt ggaacgagaa gttcctgata ttctatgttc agcattgact
    76561 gatgattgta ttggtcgtgg aaaacacctt aaagaaggtg gagcagtata tgattatata
    76621 tcaggattgc aagttggaat tgcaaatttg tcggattcat tagctgcaat taaaaaattg
    76681 gtgtttgagg aagaacgtat aagcccaagt cagctttggc atgcactgga aacagattat
    76741 gccggagaag aaggtaaggt cattcaagaa atgttgattc atgatgcacc taagtatggt
    76801 aatgatgatg attatgctga caaattggtt actgctgctt atgacattta tgttgatgaa
    76861 attgctaaat atcctaatac acgttatgga agagggccta ttggaggaat tcgttattca
    76921 ggaacatctt ctatatcagc caacgtaggg cagggacgtg gaacattagc aactccagat
    76981 ggacgcaacg cgggtacacc gttagcagag ggttgttcac catcacataa tatggatcaa
    77041 cacggcccta catctgtttt aaaatctgtt tcaaaattac caacagatga aatcgtaggt
    77101 ggggttctct taaatcagaa agtaaatcct caaacgttag ccaaagaaga agataaatta
    77161 aaactaattg ctttgttacg aacattcttt aatcgtttac atgggtacca tattcaatac
    77221 aatgttgttt ccagagagac gttgattgac gctcagaaac atcctgaaaa acacagagac
    77281 ttaattgttc gtgttgcagg atactctgca ttcttcaatg ttctttctaa ggcaacccaa
    77341 gatgacatta taggacgtac tgagcatact ttgtaaaata aagaggttct ttttatggaa
    77401 tttatgcttg acacattaaa tttagatgag attaaaaagt ggtctgaaat tttgccgcta
    77461 gctggggtaa cttcaaatcc cactattgca aaaagagagg gttctattaa tttttttgaa
    77521 cgaatcaaag atgtaagaga attgattggc tccacaccct ctattcatgt tcaggtgatt
    77581 tctcaagatt ttgaaggcat cttaaaggat gctcatgaaa ttcgaagaca agcaggagat
    77641 gatatattta tcaaagtacc tgttactcca gctggattac gtgcaataaa ggtgctaaaa
    77701 aaagagggct accatatcac tgcaacagct atttatacag ttattcaggg attattagct
    77761 atcgaagtag gagcggatta cctagctcca tattataata gaatggaaaa tctgaacatt
    77821 gattcaaatt ctgtcattcg tcaattagct cttgctattg atagacagaa ctctcctagt
    77881 aagattttag ctgcatcctt taaaaatgta gcacaagtaa ataatgcttt agctgcaggt
    77941 gcgcatgctg ttacagcagg agcagatgtt tttgaatcag ctttcgccat gccatctatc
    78001 caaaaggcgg ttgatgattt ttctgacgat tggtttgtta ctcaaaatag tcgttccatt
    78061 tagatagaga ggaaatacat atgagaattt ttgctagtcc ttctagatat attcaggggg
    78121 aaaatgcctt gtttgaaaat gccaaatcaa ttttggattt gggaaattac ccgattctat
    78181 tatgcgatca gttggtttat gatattgttg gaaaacgatt tgaagattat ctacataggt
    78241 atggtttcca tattgttctg gcgctattta atggtgaagc ttctgacaat gaaatcaatc
    78301 gagttgttgc cttggctgag aaagaaaatt gtgatagtat tatcggtctt ggtgggggaa
    78361 agacgattga tagcgcaaaa gctattgcag atttgattga aaagcctgtt attattgctc
    78421 caacaattgc atcgaccgac gcacctgtat ctgctttatc tgttatttat acagatgaag
    78481 gtgcatttga tcattatcta ttttattcta aaaatccaga tttagttttg gttgatacaa
    78541 aagttatttc acaagcccct aagcgtttat tagcgtctgg tattgcagat ggtttagcaa
    78601 cttgggttga ggcgcgtgcg gttatgcagg caaatggaaa aactatgttg ggacaacagc
    78661 aaacattggc tggagttgca attgcgaaga aatgtgaaga aacgctgttt gcagatggtt
    78721 tacaggctat ggcagcttgt gaagctaaag tggtgacacc agcattagaa aacattgttg
    78781 aagctaatac tttattgagt ggtttaggtt ttgaaagtgg aggattagct gcggcgcatg
    78841 caattcataa tggttttact gcattgacag gtgacattca tcatttaaca catggtgaaa
    78901 aagtagctta tggaacttta gtacaactat tattggaaaa tagacctaaa gaagaacttg
    78961 ataagtatat tgagttttac aaaaaaattg gtatgccaac aactctaaaa gaaatgcatt
    79021 tggatcaagt tggatatgat gatttaataa aagttggtaa acaagcaact atggagggtg
    79081 agacaattca tcagatgccg tttaagattt cgccttcaga tgttgctcaa gctattatcg
    79141 ctgtagatgc ctatgtaaat tcaaaataaa caataaggac tactgttttc caaatggtag
    79201 tcttttattg atcctcgtat tgaattctat agcagattga aataagatga gaacaaatcg
    79261 attgggaaag taaaattaat ttctataagt gttttagcaa ttgtttcgta ctatttcaga
    79321 ttcagtccac tatacgttct tcataaatca aaaagcgaca taggttgtcg gctatttatt
    79381 gtgaatacat taattagcat tccagtttta tcttcggtct aaagtaagta ttttgtgcta
    79441 tacgagataa gcgtcttgac ttactccttg atttactgca taacaatggg ataaaaagtg
    79501 ggagatagag caattcatag tcatcaaaat taatgagata cagtatacag ttttttcttt
    79561 aaacacattt caaattccct caaaaatggt ataatagtaa catcacaaaa ttggagagag
    79621 accatgagtt tttataatca taaagaaatt gagcctaagt ggcagggcta ctgggcagaa
    79681 catcatacat ttaagacagg aacagatgca tcaaaaccta agttttatgc gcttgatatg
    79741 ttcccttatc cgtctggagc tggtctgcat gtaggacacc cagaaggtta tactgcaacc
    79801 gatatcctca gtcgttacaa acgtgcgcaa ggctacaatg tccttcaccc aatgggttgg
    79861 gatgcttttg gtttacctgc agagcaatac gctatggata ctggtaatga cccagcagaa
    79921 tttacagcgg aaaacattgc caacttcaaa cgtcaaatca atgcgcttgg attttcttat
    79981 gactgggatc gtgaagtcaa cacaacagat ccaaactact acaagtggac tcaatggatt
    80041 ttcaccaagc tttacgaaaa aggcttggcc tatgaagctg aagtgccagt aaactgggtt
    80101 gaggaattgg gaactgccat tgccaatgaa gaagtgcttc ctgacggaac ttctgagcgt
    80161 ggaggctatc cagttgtccg caaaccaatg cgccaatgga tgctcaaaat cacggcttac
    80221 gcagagcgct tgctcaatga cttagatgaa ctagattggt cagagtctat caaggatatg
    80281 caacgcaact ggattggtaa atcaactggt gccaatgtaa ctttcaaagt aaaaggaaca
    80341 gacaaggaat ttacagtctt tactactcgt ccggacacac ttttcggtgc gactttcact
    80401 gtcttggctc ctgaacatga attagtagac gctatcacaa gttcagagca agcagaagct
    80461 gtagcagact ataaacacca agccagcctt aagtctgact tggctcgtac agaccttgct
    80521 aaggaaaaaa ctggtgtttg gacaggtgcc tatgccatca accctgtcaa tggtaaggaa
    80581 atgccaatct ggattgcgga ctatgttctt gctagttatg gaacaggtgc ggttatggct
    80641 gtgcctgccc acgaccaacg tgactgggaa tttgccaaac aatttgacct tccaatcgtc
    80701 gaagtacttg aaggtggaga tgtcaaagaa gctgcctaca cagaggatgg cctgcatgtc
    80761 aattcagact tcctagatga actcaacaaa gaagacgcta ttgccaagat tgtggcttgg
    80821 ttggaagaaa aaggctgtgg tcaggagaag gttacctacc gtctccgcga ctggctcttt
    80881 agccgtcaac gttactgggg tgagccaatt ccaatcattc attgggaaga tggaacttca
    80941 acagctgttc ctgaaactga attgccgctt gtcttgcctg taaccaagga tatccgtcct
    81001 tcaggtactg gtgaaagtcc actagctaac ttgacagatt ggcttgaagt gactcgtgaa
    81061 gatggtgtca aaggtcgtcg tgaaaccaac actatgccac aatgggctgg ttcaagctgg
    81121 tactacctcc gctatattga tccacacaat actgagaaat tggctgatga ggacctcctc
    81181 aaacaatggt tgccagtaga tatctacgtg ggtggtgcgg aacatgctgt acttcacttg
    81241 ctttacgctc gtttctggca caaattcctc tatgacctcg gtgttgttcc gactaaggaa
    81301 ccattccaaa aactctttaa ccaagggatg attttgggaa caagctaccg tgaccaccgt
    81361 ggtgctcttg tggcaaccga caaggttgaa aaacgtgatg gttccttctt tcatgtagaa
    81421 acaggggaag agttggagca agcgccagcc aagatgtcta aatcgctcaa gaacgttgtt
    81481 aacccagacg atgtggtgga acaatacggt gccgataccc ttcgtgttta tgaaatgttt
    81541 atgggaccac tcgatgcttc gattgcttgg tcagaagaag gtttggaagg aagccgtaag
    81601 ttccttgacc gagtttaccg tttgattaca agtaaagaaa tccttgcgga aaacaatggt
    81661 gctcttgaca aggtttacaa cgaaacagtc aaagctgtta ctgagcaaat tgagtctctc
    81721 aaattcaaca cagctattgc ccaacttatg gtctttgtca atgctgctaa caaggaagat
    81781 aagctttatg ttgactatgc caaaggcttt attcaattga ttgcaccatt tgcacctcac
    81841 ttggcagaag aactctggca aacagtcgca gaaacaggtg agtcaatctc ttatgtagct
    81901 tggccaactt gggacgaaag caaattggtt gaagatgaaa ttgaaattgt cgtccaaatc
    81961 aaaggaaaag tccgtgccaa actcatggtt gctaaagata tatcacgtga agaattacaa
    82021 gaaatcgctt tagctgatga aaaagtcaaa gcagaaattg acggtaagga aatcgtgaaa
    82081 gtaattgcgg taccgaataa actcgttaat atcgtcgtta aataacgagt ttattagctc
    82141 tatctgccac cttcaatagt ccactggact attgaagcca actaaattag ttaacattgt
    82201 tgtgaaataa gataggaatc cttcagagta gaatctggag gattttttga accttcttat
    82261 gaaagtatga tatatagtag actgaatcta aaatagtacg aaacaattgc taaaacattt
    82321 atagaaatta attttacttt cccaatcgat ttgttctcat cttatttcaa tacgctatac
    82381 tatgggcaac tataaagttt gaaaagcgaa acaaggagaa agttatacca gtaaacgaat
    82441 atggtcagat gattggtgag tcaatggaag gttatacacc aggtgaactg acttctattg
    82501 atctcttaga agggcgttat gctaggatag aggctctttc agtggaaaag catgcggagg
    82561 atttgctagc tgtttatggt cctgatactc ctcaggagat gtggacctac ctctttcagg
    82621 agccagtagc agacatggag gaactggtca gccttttaaa tcagatgttg gctcgtaagg
    82681 accgttttta ctatgcaatc atagacaagg caactggtaa ggctttggga actttttctc
    82741 tcatgcgtat tggccagtaa cggagtaata gaagtgggag ctgttacttt ttctccaaaa
    82801 ctcaggggga cacggatagg gacagaagcc cagtatctac tggctcgcta tgtctttgaa
    82861 gagcttaact atcgtcgtta tgagtggaaa tgcgatgctc ttaacctgcc atccagacgt
    82921 actgcggagc gtttgggctt tatttatgaa ggaaccttcc gtcaggcagt ggtttataag
    82981 gggcgtacga gagatacgga ttggttgtct atgattgata aggactggcc caaagtcaaa
    83041 gatcgtttgg aaacatggtt gcgtcctgaa aattttgata aaaatggaca gcagtacaag
    83101 agcttgagag agttttaaga ggtgttgaca tgatcactat tagaaagcaa gaaatcgtca
    83161 agttcgagga tgttttgcat ctctatcagg ctgtcggttg gacaaattat ataaatcaac
    83221 atcagatgct ggagtagacc ttgtatcatt cattaataat ttatctggca cttgatggtg
    83281 atgctgttgt gggcttgatt cgtttggttg gagatggttt ttcatcagtt tttgtacagg
    83341 atttgattgt tttgtctagc tatcagcgcc aagggattgg tagctccttg atgaaacagg
    83401 ctttaggaaa ttttaaagag gcctatcaag tccagctggc gacagaacag acagaaaaaa
    83461 acgtggaatt taatcgttct atgggctttg aaattttatc cacctataat tggataggga
    83521 tgacttggat gaatcgtaaa aaataacaaa acttgttttt tcttaagcaa aatttaagga
    83581 tggtctagta ttatataatc attaaataaa gacctcctaa ctttatttaa taaaatccta
    83641 aacttttttc atcacaatct cctaatgaag ccacccaatc aggtggcttt tttgcgtggt
    83701 gaggtgctgg tgatagaaat tttttgcaaa atggtaaaat tttagaaaag ttaagctagt
    83761 tttaagtttc gtcttgtatc atgtagttat taaataaaga cctcctaact ttatttaata
    83821 aaatcctaaa cttttctttt tcataataat ctcccttaac tccacccaat caggtggagt
    83881 tttttagctc tatttcaggc ttttggggac tattctaaaa ataatttttc gatatttttc
    83941 ggtatttttc ggattttggt cggggaattg gcggggactt ttttagcgaa tatgactaag
    84001 aaataggtct gtggtcgctt cagctagttc aaactcaatt tgattgtaag tgactgttaa
    84061 ttgaaggact ggaactactg taataatgat tttgcttgtc tagtagtggc gtattaaatg
    84121 agaagtgaca tgtaagattg tctcttcgtt tacgaaatca aaatttctat gaaattgata
    84181 gaatccctaa cgttctctta atcttaaccg tcttgtcgta cagcggttga agaaaatgta
    84241 aacctttgac aaaattcgtg aactatggga taataaaaag gaattgagta ctagttctat
    84301 atcataggct agaaaagacc ccaagctcac gaccaaataa gcttgaggtc ttttttgaca
    84361 ctatttgtcc ttgtttagcc atttatcgac tatacgaaga acgacaccga ccacaattgg
    84421 tccgatgata gttttgagta ctaattccat catgggctat ctcacctcct ttcgtaggcg
    84481 gtgtagaagt gccgtataat attataccac ataagtgcta aacttaggag tcacccaatc
    84541 aggtggcttt tttgtttgtg gcttgttttt ttgatataat agagccatga gtagaatttt
    84601 agataatgag atgatgggtg atgaggagtt agtagaacgc acgctccgtc ctcagtattt
    84661 acgtgaatat attggtcagg ataaggttaa ggatcagctt cagatcttta tcgaagccgc
    84721 taaaatgcgg gatgaagcgc tggatcatgt gctcttattt ggccctccag ggcttgggaa
    84781 aacaaccatg gcttttgtca ttgccaatga actgggagtc aatctcaagc aaacgtctgg
    84841 tcctgttatc gaaaaagcgg gtgatttggt agcgattttg aatgagttag agcctggaga
    84901 tgtcctcttt attgacgaga ttcatcgctt acccatgtca gtggaagaag tgctttatag
    84961 tgccatggag gacttctaca tcgatattat gattggggct ggtgaaggca gtcgcagtgt
    85021 tcatttggag ttgcctcctt ttaccttgat tggtgcgacg actcgggctg gtatgctctc
    85081 aaatccacta cgggcacgtt ttgggattac aggtcatatg gagtattatg cccatgctga
    85141 cttgacagaa attgtcgagc ggacggcaga tatttttgag atggaaatca ctcatgaggc
    85201 agcatctgag ttggccctac gtagtcgtgg aacccctcgt attgccaatc gtctcctcaa
    85261 gcgcgtgcgc gattttgccc agataatggg gaatggggta atcgatgata ttattaccga
    85321 taaggctttg actatgctgg atgttgacca tgaaggtttg gactatgtgg atcaaaaaat
    85381 ccttcgtacc atgattgaga tgtacagtgg aggacctgtt ggtctaggaa ctctttctgt
    85441 caatatcgcc gaagagcgtg agacagttga agacatgtat gagccttact tgattcaaaa
    85501 aggttttatc atgcggacac ggtctggacg ggtggcgact gctaaggcat atgagcactt
    85561 aggttatgaa tacagtgaaa aataagcaag aaatcttaga ggcttttaga gaaaatccag
    85621 atatgatggc cattctgacg atcatccgag accttggttt gaaagactcg tagttggcag
    85681 caggttctgt cagaaatttc atctggaatc ttttgtcaga caaatcccct tttgatcatg
    85741 aaacagatat agatgtgatt ttctttgatc cagatttttc ttatgaggaa accttattac
    85801 tggagaaaaa gctgagagag gattttcctc agtaccagtg ggaattgaaa aatcaggtct
    85861 atatgcatca gcacagtcct cacactgctt cctataccag ttctcgtgat gctatgagta
    85921 agtatccaga acggtgtacg gcagttggac tgcgcttgaa tgaagaatca gattttgaac
    85981 tctatgcacc ttatggtttg gaggatattt tgaattttca agttcgtcca actcctcatt
    86041 tcttagaaaa tgaagaccga atggaactct atcaaacacg tttatccaag aaaaattggc
    86101 aggagaaatg gaaaaatttg atttttaaaa atacttaagg aaactttaag ctagggagtg
    86161 tacactaagt tcataagtta agaagacctt aacttaaact cctaaaactt tttcataata
    86221 atctccctat aaaaataaag tcgcccaatc aggcggctta tttttttgaa aaatgggcat
    86281 ggtgcctgag aataaatagc ttagtgatag aagaaaatgg ggaaatatgg tataatgaaa
    86341 cgatagattt ttgaatagga ataagatcat gtttggattt tttaagaaag ataaggctgt
    86401 ggaagtagag gttccgacac aggttcctgc tcatatcggc atcatcatgg atggcaatgg
    86461 ccgttgggct aaaaaacgta tgcaaccgcg agtttttgga cataaggcgg gcatggaagc
    86521 attgcaaacc gtgaccaagg cagccaacaa actgggcgtc aaggttatta cggtctatgc
    86581 tttttctacg gaaaactgga cccgtccaga tcaggaagtc aagtttatca tgaacttgcc
    86641 agtagagttt tatgataatt atgtcccgga actacatgcg aataatgtta agattcaaat
    86701 gattggggag acagaccgcc tgcctaagca aacctttgaa gctttaacca aggctgagga
    86761 attgactaag aacaacacag gattgattct taattttgct cttaactatg gtggacgtgc
    86821 tgagattaca caggcgctta agttgatttc ccaggatgtt ttagatgcca aaatcaaccc
    86881 aggtgacatc acagaggaat tgattggtaa ctatctcttt actcagcatt tgcctaagga
    86941 cttacgagac ccagacttga ttatccgtac tagtggagaa ttacgtttga gcaattttct
    87001 tccatggcag ggagcctata gtgagcttta ttttacggac accttatggc ctgattttga
    87061 cgaagcggcc ttgcaggaag ctattcttgc ctataatcgt cgtcatcgcc gatttggagg
    87121 agtttaggag gaaatatgac ccaggattta cagaaaagaa ccttgtttgc agggattgcc
    87181 ctggctattt tcctaccaat tttaatgatt gggggcctct tgcttcagat agcaatcgga
    87241 atcatagcca tgctagccat gcatgaactt ttgaagatga gaggtctaga gaccatgacg
    87301 atggagggcc tcttgaccct ctttgcaacc tttgcattga ccattccctt ggagaattac
    87361 ctgacttttt tgccagttga tgggaatgtg gttgcctata gtgttttgat ttcaatcatg
    87421 ttaggaacga ccgtttttag caagtcttat acgattgagg atgcggtttt ccctcttgct
    87481 atgagcttct acgtgggctt tggatttaat gctttactag atgctcgtgt tgcaggtttg
    87541 gacaaggctc tcttagcctt gtgtatcgtc tgggcgacag acagtggtgc ctatcttgtt
    87601 gggatgaact atgggaaacg aaagttagca ccaagggtat cgcctaataa aacccttgag
    87661 ggtgccttgg gtggtatttt aggagcaatt ttagtaacca ttatctttat gatagttgac
    87721 agtacagttg ctcttccata tggaatttac aagatgtcag tctttgctat tttctttagc
    87781 attgctggac aatttggtga tttactagaa agttcgatca aacgtcattt tggtgttaag
    87841 gattctggga aatttatccc tggacatggt ggtgttttgg atcgtttcga tagtatgttg
    87901 cttgtatttc caatcatgca cttatttgga ctcttttaat caaaagacgg aggaaacgct
    87961 atgctcggaa ttttaacctt tattctggtt ttcgggatta ttgtagtggt gcacgagttc
    88021 gggcacttct actttgccaa gaaatcaggg attttagtac gtgaatttgc catcggtatg
    88081 ggacctaaaa tttttgctca cattggcaag gatggaacgg cctataccat tcgaatcttg
    88141 cctctgggtg gctatgtccg catggccggt tggggtgatg atacaactga aatcaagaca
    88201 ggaacgcctg ttagtttgac acttgctgat gatggtaagg ttaaacgcat caatctctca
    88261 ggtaaaaaat tggatcaaac agccctccct atgcaggtga cccagtttga ttttgaagac
    88321 aagctcttta tcaaaggatt ggttctggaa gaagaaaaaa catttgcagt ggatcacgat
    88381 gcaacggttg tggaagcaga tggtactgag gttcggattg cacctttaga tgttcaatat
    88441 caaaatgcga ctatctgggg caaactgatt accaattttg caggtcctat gaacaatttt
    88501 atcttaggtg tcgttgtttt ttgggtttta atctttatgc agggtggtgt cagagatgtt
    88561 gataccaatc agttccatat catgccccaa ggtgccttgg ccaaggtagg agtaccagaa
    88621 acggcacaaa ttaccaagat tggctcacat gaggttagca actgggaaag cttgatccaa
    88681 gctgtggaaa cagaaaccaa agataagacg gcaccgactt tggatgtgac tatttctgaa
    88741 aaggggagtg acaaacaagt cactgttaca cccgaagata gtcaaggtcg ttaccttcta
    88801 ggtgttcaac cgggggttaa gtcagatttt ctatccatgt ttgtaggtgg ttttacaact
    88861 gctgctgact cagctctccg aattctctca gctctgaaaa atctgatttt ccaaccggat
    88921 ttgaacaagt tgggtggacc tgttgctatc tttaaggcaa gtagtgatgc tgctaaaaat
    88981 ggaattgaaa atatcttgta cttcttggca atgatttcca tcaatattgg gatttttaat
    89041 cttattccga ttccagcctt ggatggtggt aagattgtgc tcaatatcct agaagccatc
    89101 cgccgcaaac cattgaaaca agaaattgaa acctatgtca ccttggccgg agtggtcatc
    89161 atggttgtct tgatgattgc tgtgacttgg aatgacatta tgcgactctt ttttagataa
    89221 tcgaggaata ttatgaaaca aagtaaaatg cctatcccaa cgcttcgcga aatgccaagc
    89281 gatgctcaag ttatcagcca tgctcttatg ttgcgtgctg gttatgttcg ccaagtttca
    89341 gcaggtgttt attcttatct accacttgcc aatcgtgtga ttgaaaaagc taaaaacatc
    89401 atgcgccaag aattcgaaaa gattggtgct gttgagatgt tggctccagc ccttcttagt
    89461 gcagaattgt ggcgtgaatc aggtcgttac gaaacctatg gtgaagacct ttacaaacta
    89521 aaaaaccgtg aaaaatcaga ctttatctta ggtccaactc acgaagaaac ctttacagct
    89581 attgtccgtg attctgttaa atcttacaag caattgccac tcaaccttta tcaaattcag
    89641 cccaagtatc gtgatgaaaa acgcccacgt aatggacttc ttcgtacacg tgagtttatc
    89701 atgaaggatg cttatagttt ccacgctaac tatgatagtt tggatagtgt ttatgatgag
    89761 tacaaagcag cctatgagcg tattttcact cgtagtggtt tagacttcaa ggctattatt
    89821 ggtgacggtg gagccatggg tggtaaggat agccaagaat ttatggccat tacatctgct
    89881 cgtacagacc ttgaccgctg ggttgtcttg gacaagtcag ttgcctcatt tgacgaaatt
    89941 cctgcagaag tgcaagaaga aatcaaggca gaattgctca aatggatagt ctctggtgaa
    90001 gataccattg cttactcaag tgagtctagc tatgcagcta acttagaaat ggcaacaaac
    90061 gagtacaaac caagcaaccg tgttgtcgct gaagaagaag ttactcgtgt tgcaacgcca
    90121 gatgttaaat caattgatga agttgcagct ttcctcaatg ttccagaaga acaaacgatt
    90181 aaaaccctct tctacattgc agatggtgag cttgttgcag cccttctagt tggaaatgac
    90241 caactcaacg aagtcaagtt gaaaaaccac ttaggagcag atttctttga cgttgctagc
    90301 gaagaagaag tggcgaatgt tgttcaagca ggatttggtt cacttggacc agttggtttg
    90361 ccagagaata ttaaaattat tgtagatcgt aaggtgcaag atgttcgcaa tgcagttgtc
    90421 ggtgctaacg aagatggcta ccacttgact ggtgtgaacc caggccgtga ttttactgca
    90481 gaatatgtgg atatccgtga agttcgtgag ggtgaaattt ccccagatgg acaaggtgtc
    90541 cttaactttg cgcgtggtat tgagatcggt catattttca aactcggaac tcgctactca
    90601 gcaagcatgg gagcagatgt cttggatgaa aatggtcgtg ctgtgccaat catcatggga
    90661 tgttacggta tcggtgtcag ccgtcttctt tcagcagtga tggagcaaca cgctcgcctc
    90721 tttgttaaca aaacgccaaa aggtgaatac cgttacgctt ggggaatcaa tttccctaaa
    90781 gaattggcac catttgatgt gcatttgatt actgtcaatg tcaaggatga agaagcgcaa
    90841 gccttgacag aaaaacttga agcaagcttg atgggagctg gttatgaagt cttgacagat
    90901 gaccgtaacg aacgtgtcgg tgttaaattt agcgatagtg acttgattgg tcttccaatc
    90961 cgtatcactg ttggtaaaaa agcggccgat ggcattgtag aagttaagat caaggcaact
    91021 ggtgacacca ttgaggttca tgcagataac gtgcttgaaa cgcttgaaat cctaagcaag
    91081 aaataaaaac tataatcaga agaaaaacaa ggaaaaaatg taactagttt ttaccttgtt
    91141 ttttctgtat aatgggagaa atgagtagat aaagaggtaa atgttatgct aagatttcca
    91201 aaggattttg tctggggatc ctctacttct ggaccgcaga cagaaggacg tgtagctggt
    91261 gacggtaagg gagacaatct ctgggattac tggttccaag tggagccaaa tcgttactat
    91321 aatgggattg gttcagataa gacatcgact ttttatgaaa attgggagcg ggatattgag
    91381 cttttgttag agactggtca cacagtcttt cggacttcta ttcagtggtc acggattttt
    91441 ccacaaggct gtggaaaagt caaccctcaa ggtgtggatt tttatcgtaa ggtctttgag
    91501 gctattaagg ctaaaggaat tcgtctgtta gtcaatctct atcattttga tttacctttt
    91561 gcccttcaag aggatggtga tggttgggaa aataaggcga cagtctcagc ctatgaagac
    91621 tatgctcgtt tttgttttga gacttatgga gatttagtgg atcaatggat tacctttaac
    91681 gagcccatcg ttcctgtaga atttggttat ttttatgatg cccattatcc acataaggtg
    91741 gatgcagagg cagctgttaa ggtagcctat catacacaat tggccagcag ccgggctgtt
    91801 aaggcttgcc atgaactttt gcctgattcc aagattggga ttgtcctcaa cttgacaccg
    91861 gcttatccac gtagccagca tcctgctgat gtcaaggcag ctcgtattgc ggcccttttt
    91921 caggcccaat ctttcttaga tccatctgtc ttggggactt atccacagga gttggtagaa
    91981 atcttgcatg aacacggtct tttacctgat gctacagagg aagagttgga actcattcat
    92041 gataatacgg tggacttcct tggtgtgaac tactatcaac ctttgcgtgt tatggctcct
    92101 cgatttgcta agcatccaga gagtccactc ttaccagaac atttttacga gccttatgtg
    92161 atgcctggac gtaaaatcaa ttctcaccgt ggttgggaga tttatgagca agggatttat
    92221 gacatcgccc aaaatatcaa ggaaaattat ggcaatattg aatggatgtt gactgagaat
    92281 ggtatgggtg ttgaagggga agaaaaattc cgtcaagatg gaatgattca agatgattac
    92341 cgtattgact ttgtaaaagg tcatcttcgt gaacttcacc gtgccattga agatggtgcc
    92401 aactgtaagg gctacttgat ttggaccttt attgactgct ggtcatggct caacagctat
    92461 aaaaatcgct atggtttggt cgaattagac ttggaaacgc aagaacgtcg tctgaagaaa
    92521 tcaggccact ggttcaaaaa attaagcgat aataatggat tttaaaaagg actctgactg
    92581 attatcccaa tcggtcagag ttttttgctt acaggagctt aatttgaact ataccaattt
    92641 ttactcttga caagtgaaag aaacaagtat atactgtttt tgctgattct ataaaagatg
    92701 aattagacta taccaatttt aaggagaaag cacagcttgc ctgtgtcgta tatactatgt
    92761 gtggaattgt tggtgttgtt ggaaacacaa atgcaactga tattttgatt caagggcttg
    92821 aaaagcttga ataccgtggc tatgattctg cgggaatttt tgtcctagat ggtgctgata
    92881 accatttggt gaaggctgtt ggtcgtatcg cagaattgtc tgccaagaca gctggtgttg
    92941 agggaacaac tggtatcgga catactcgtt gggcaactca tgggaaacca acggaagaca
    93001 atgctcaccc acaccgctct gagacagaac gttttgtctt ggttcacaat ggggtgattg
    93061 aaaactacct tgaaattaaa gaagaatacc ttgctgggca ccacttcaaa gggcaaacag
    93121 atacggaaat cgccgtacat ttgattggaa aatttgcgga agaagacggt ctctcagttc
    93181 ttgaagcctt taaaaaagct cttcatatta tccgtggttc atatgccttt gccttgattg
    93241 actctgaaaa tccagatgtc atctatgtag cgaaaaacaa atctccactt ttgattggtc
    93301 ttggggaagg ctacaatatg gtctgctcag atgctatggc tatgattcgt gaaaccaacc
    93361 aatacatgga aattcatgac caagagttgg taatcgtcaa ggctgatagc gtggaagttc
    93421 aagactatga tggaaatcgt cgtgagcgtg ctagctacac tgctgaactt gacttgtcag
    93481 atatcggtaa gggaacttac ccttactaca tgctcaagga aattgacgag caaccaactg
    93541 ttatgcggaa actcatccaa gcctacacag atgaggctgg tcaagttgtc gtagacccag
    93601 atatcattaa ggctgttcaa gacgcagacc gcatctacat ccttgcagct ggaacatctt
    93661 accatgcagg atttgcttct aagaagatgt tggaagaatt gacagataca ccagttgaac
    93721 ttggaatctc atctgagtgg ggctacggta tgccacttct cagcaagaaa ccactcttca
    93781 tctttatcag ccaatctggt gaaacagcgg atagtcgtca agttttggtc aaggctaatg
    93841 aaatgggaat tccaagctta acagtgacaa atgttccagg ttcaaccctc tcacgtgaag
    93901 ccaactatac catgctcctt cacgcaggtc ctgaaattgc cgtggcatca actaaagcct
    93961 atacagcgca aatcgcagcc cttgccttcc ttgcaaaagc agtcggagaa gcaaatggta
    94021 atgctaaagc gcaagccttt gacctggttc atgaattgtc aatcgtagct cagtctattg
    94081 aatcaactct ttcagagaaa gaaaccattg aagccaaggt tcgtgaactt cttgaaacaa
    94141 ctcgtaacgc cttttacatc ggacgtggtc aagattatta cgtagccatg gaagcaagtc
    94201 tcaaactcaa agagatttct tatatccagt gtgaaggttt tgcggcagga gaactcaagc
    94261 acggaaccat tgccttgatt gaagaaggaa cgcctgtttt ggctctcttg tcagatccag
    94321 ttcttgccaa ccatactcgt ggaaatatcc aagaggtcgc agcccgtggt gccaaagtcc
    94381 tcactatcgc agaagagaat gtagccaaag ataccgacga tatcgtcctt acgaccgtac
    94441 atccatacct ctcaccaatt tcaatggtcg taccaacgca attagtcgct tactttgcaa
    94501 ccctccaccg tggcctcgat gtggacaaac cacgtaacct tgccaagtca gtaacggtag
    94561 aataagctaa aaaagtctag ttatctaggc tttttcttgt gagcaaattg gttgcttgta
    94621 ctcaagattc gtgttatact cttagaaaat ctcttcaaac cacgtcaacg tcgccttgcc
    94681 gtaggtatgg ttactgattt cgtcagttct atccacaacc tcaaaacact gttttgagca
    94741 acctgcggct agcttcctag tttgctcttt gattttcatt gagtgttaga ataatttttc
    94801 aaattgaagg acagagatag acaaggagaa tcacaatggt agaattggga atttcaacat
    94861 ttggggaaat aacggagctt gaagggactg gacaaactta cagtcatgct gaacgcattc
    94921 gccagttggt ggcagagatt gagctggctg acaaggttgg tttggacgta tatgggattg
    94981 gcgagcacca tcgggcggat tttgcagtat cagccccaga gattgtcctg gccgctgggg
    95041 cagtcaatac caagaaaatc cgtttgacca gtgcagtcag cattctgtca agcatggatc
    95101 cgattcgttt gttccaacag tatgccacta tcgatgcttt gtcaaatgga cgagcggaga
    95161 ttatggctgg aaggggctct ttcacggaat ctttccctct gtttggttat gacttgaaag
    95221 actacgactc tctttttgat gagaaattag acttgcttca gttagtcaat gaaaagacca
    95281 agttagactg gcaaggtcga ttgactcaaa cgattgctgg taaagaagtt tatcctcgtc
    95341 cagttcagga caaattgccc ttgtggatag ctacaggtgg tcatgtcgaa tcaacagtga
    95401 agattgctca ggctggtcta ccgattgtat atgctattat tggtggtaat ccacgttatt
    95461 ttaaaaagtt gattcaggct tatcgtgaga ttggaagcga agcggtccat gctgacaaag
    95521 acttgaaagt gggagcccat tcttgggggt ggattgcgga agatggtgag caggcggtga
    95581 aagattattt ccatccgact aagcaagtgg tggatgctat ttccaaagac cgtccgcact
    95641 ggcaggaatt gcgttatgag caatatttgg agcaggtagg gtcaaatggc gccatgtttg
    95701 ttggcaatcc agatcaggtg gccgaaaaat tgattcgcat gatagaggat ttaggattgg
    95761 accgcttcat gcttcatcta ccgcttggtt ctatgcctca tgaccaggtt ctgagagcta
    95821 ttgaactctt cggaacacaa gttgcaccca aagtacgagc ttattttgct atgaaagagg
    95881 cttaataaaa aatcctaatc aagtttatta ggacaacaaa tattgtacga aaattaatcc
    95941 cccgagctat aatgcttggg ggatttttct ataggagggc tagtttagtt ctctttttta
    96001 ttttttagta agaaaccgag accaaggagc gcaaggaggc tgattcctgc aaataggagt
    96061 ttgttttcgt ttttaattcc tgtatttgga agttcagctt gtctatctgg ggttgcaggt
    96121 atatttttct tgctagagtt ttctaccgat tcgtttcgaa ccgcttcttt tacagatgat
    96181 gcttgtgctt cttgtgctgg ttgttcagct tgtgaatcag ctgtagcttg gctaggttta
    96241 ttttccgcat cagctacttt ggtatctggt ttagtagaat caggtctagc ttctggagct
    96301 ggatcttgat gttcagggtg agaagcttca ttttgatgtt caggcttgga aggggttgag
    96361 tctggtttct cttctgcagt tgactcatgg ctagttccat tttgagagac tcgaagaaca
    96421 gtagctgtaa gggcattcaa tttcaagcct ttttcagtcc attcaagtcc tttcgggttg
    96481 gcaattccga ctgatcctgc ttggttttca tctgccaaaa cttccgcatt tcttagatgt
    96541 gcaaaggcag ttcccaaatt aaattcgcga gctttttcat ccgcattgac aaagactgcg
    96601 tagatatcgc cgtttggagc agtgatttga tagccaatca ctacatcctc tttttccaca
    96661 ccattttggc ctgggacagt gatgaggtgg acacggtctt tgatatcttg aagactctta
    96721 agtcggaagg catctgtaga ttgacgaagg gcaatcaaac ctttcatata gtcacggctc
    96781 ttgacatttt caggataagc tttaccatct gtagccttag tccagtcaaa cttgttgact
    96841 gcatcactag aatcgtaaga gtcatggatg aagtaaggat agtcaaatgg gttgccgtcc
    96901 ttatcacgca acaagtgaga tttgtttgga accttatcct ctgctactgg agtcttgtag
    96961 gctgggtcac ggaattgttt agtacgtcca tattcctgac cggagtggat aaatggagtt
    97021 ccttgagctg tcaagaccat gagatttcca agtcgtaaac gacggtggat ttcagcatag
    97081 ttctcagcct tgcttgggtc ttttttgata gactgggcaa tgatgtcaaa gagggtcaag
    97141 ttatcatggg ctgcgatgta ttggatgaca tctccagggc tgtcagcttc aaagttagtt
    97201 ggttgagcaa tgagattttt aaagatggtg ttgacatcac gcttgccacc tgtgataaag
    97261 gcaggttgac cttcgtttgg ataaccagat ttgaggttgt tacggatgtc atctgaaaag
    97321 acagcgacag tatcggtatg tttcatccaa tcttggtcag cagctttagt aggcatgttt
    97381 tcatcaccgg cataggttct ccaaccttca ccaagcatga tgaggtttgg attgagggcg
    97441 cgtgcagcct tgtaagcttc ttcgatagaa gcggcgtcat ggtctcccat catatcgaaa
    97501 cggaagccat ccactttgta ggtatcaact aggtatttga tagagtcaat taggagccgt
    97561 ttggtcatat ggtgggttgt ccccaagcgt ccaccaccaa agctagttcg aggtgtgcca
    97621 tcggcatcca taaagtggta gtagtttggt tccaaatctt caaagagatc gactttggct
    97681 gtgtggttat aaacgacatc taggatagct cccataccac gtttgtggat ttcgttgatg
    97741 aggtttttaa attctgcgat tcgtttttct ggattcttag gatcgcttga gtacatacca
    97801 gtcaaggaga agtagttttg agggtcatat ccccagttgt agttgctgtt gcttgaagcg
    97861 tagtcagaca agcgttcatg gtttttcaat tcattgacaa agtagtaaga caagactgga
    97921 aggagctgga tatgggttac acccaagtct ttgagatagt ctagtttttc aatgaaggct
    97981 tcaaaagtcc caaatggttt ggtcaagtct tttgcaatgg caggatctga agtgaaatca
    98041 cgcacatgag cttcgtagat aacggcgtct tcacgagtct tgaaattgtg aatcttacca
    98101 taagtcaagt cttgaggtcc gagtttagct ggatctacaa aggcggcttt agccacttta
    98161 tgggcatcgt caatcttgga atcgtcgcta ttccaagcag caagagattt agcgtaagga
    98221 tcgagtgcaa gaacagtttt accttgacgc tcgatttggt attgataata gtagccagtg
    98281 aaatctgtga ttccgagttt gtttgtgctg tctagagttt gtttccaagt tcctctttcc
    98341 cctttttcaa gagcgacagt tccaactact ttgtcagggt cattcttgtc gtagacaaca
    98401 acagaaacct tatcagcact tggtgaccaa agggtcaaat caacttgttt tccttcttct
    98461 tttaggtcag ctcccagttt gccatcatag ctgtatgtct catctttcag gcgccagctt
    98521 gttttggtag tgaattggtc ggaattgtag ctaacagtat aaggatgttt tgtgtcagag
    98581 aaatctccgc tgtaggtcac tttcttacca gcttcatcga ttgcaacatc ggtaatagtt
    98641 accttgtttc ctaggtgatt agtgatgttg gagtgtttga ggatatcttc ttttttagca
    98701 ccgacaagtg ttgaaaagct actttcaatg ctagaagtgc ctacgtgttg ggctcctgtc
    98761 atacggatat catggacata gtatggattt gtgtaaatcg attcatcatc gtcttttagg
    98821 aaaatttggc tatgattttt caaatctgtg aacttataat tttctttacg gattttcacg
    98881 tcgtctcctt gtttgctctc atctagtaat aaaaatccaa attctcttgc ggcttcatta
    98941 agaggaatgt cgatatagcg gccatatttg cctgtagccg taaagtctgt tccgtcaggc
    99001 cattgagcgc tacttggatt tttcacatct ccccagtacc agagagattt cttgtcatag
    99061 ttgccatctg tgcggtagta gttgacgcga acagttcctg caggctgtgg ctcgtaagag
    99121 aaaaccttgt aatcttggtc taaccaagct tcgttcattt ttggaactag tttttctaca
    99181 gatttatcgc cggttagatt ttttccagct gtattgttga tgaggaagct aattttcttg
    99241 gcttgttctc cctttaattt gacatctagg taatagccgt agtcatcttt cttggcatcc
    99301 ttgaaggaca aagctccgtt tggccagttt tcagatggtt tttcaacatc gtcccaagtc
    99361 catagtcctt gagcatcctt gttttcttca ggaagttttt tgacatggat acggaagtag
    99421 ttatcttcga tgggctcttc tgccttagtt tcagttgtta ctggactagt agaagtagtt
    99481 ggctcacttg aacgatcttg tccagtttgt ggtgctggat cggctgggct tgcagcaggt
    99541 ttttcttctg ttttctctag ggaagcgttt gcatttccag gtgaagaaat atcactttcg
    99601 ttcttaccag agccagctgc tgtatcagta agaggttggg caagggtggt tgtatttctg
    99661 ttgttgggtt cgttagtggt tggtgtactt tcgtcggatg agatagttgg tgtagccatg
    99721 gcaagcaaga caaggcttgc gccgataagg acagaaccag ttccattttt gagggaacgg
    99781 atgctgtaga ccattttttt ctcagtgtga gatggtgttt ttctcataat gattctccaa
    99841 tcaataaatt tctatatcag tatagcatgt agtaaaacaa tatgcaagcg ttttctttga
    99901 tttgtgaaag aaattcttac ttttgagaag ttttccgtac tattttatat atcagtataa
    99961 atgacggaga taaaaatgtc acgtaagcca agccgtgtta ttaggaagat gatgtgttct
   100021 cagctatttt tattcagaaa ttgatctcta acattaccaa taaaaaagaa aaatatcttg
   100081 acaagcaagg gaaaattttg ttacaataat agacggtact ttttactttt ggtctctcaa
   100141 gagtgtacag ggacgtgctg acaaatgttg caaaagtaca cacagatgat atctgtcacc
   100201 aagtgtatca tcaccaaaaa taaaaaaaca caggagaatg tagatgccta caattaacca
   100261 attggttcgc aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag ctttgaacgt
   100321 tggttacaat agtcataaaa aagttcaaac aaacgtttct tcaccacaaa aacgtggtgt
   100381 tgcaactcgt gttggaacaa tgacacctaa aaaacctaac tcagcccttc gtaaattcgc
   100441 tcgtgtacgt ttgagcaacc ttatcgaagt tactgcctac atcccaggta tcggacacaa
   100501 cttgcaagag cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc ttccaggggt
   100561 acgttaccat atcgtccgtg gtgcacttga tactgcaggt gttaacgatc gtaaacaagg
   100621 ccgttctaaa tacggtacta aacgtccaaa agcataagga aaggggataa agagaaatga
   100681 gtcgtaaaaa tagagctcca aaacgtgacg tattgccaga tccgctatac aattcacaac
   100741 tagttactcg tcttatcaac cgcgttatgc ttgatggtaa acgtggtact gctgcttcaa
   100801 tcgtttacgg tgcttttgag caaatcaaag aagctactgg caacgatgca cttgaagtat
   100861 ttgaaacagc tatggaaaac atcatgcctg tacttgaagt acgtgcacgt cgtgttggtg
   100921 gttctaacta ccaagtccca gttgaagttc gtccagaacg tcgtacaaca cttggacttc
   100981 gttggttggt aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac cgtcttgcaa
   101041 aagaaatctt ggatgctgct aacaacactg gtgcagcagt taagaaacgt gaagatactc
   101101 accgtatggc tgaagctaac cgtgcattcg cacacttccg ttggtaagat aggatgcgaa
   101161 agcgttaaga aagtcccaga gaaaataggg aatcgaagca ggttgcggtt gcaaccaatg
   101221 agattcatct ttttctccag acttttagct tgagctcaac taaatcatga tgctaggaac
   101281 ggtaaggatg caaggtaaaa ataggaaact gacgcagtat tcgacgaata caaggagttt
   101341 tatctttttc acgcagcatc ccgttccagc tcacatcggc taactaactt tagcccgggt
   101401 tcaaattagc taaatcgatt agtattagct ataactcagc ttaccatctc gtaagttgaa
   101461 accaacaata gcatgaaaac attgagaacg ggtaggtcct gcctatccgt ttttattaaa
   101521 atcgtgttat aatagaatag aaatcaaaaa taaataggag aaacaaacct catggcacgc
   101581 gaattttcac ttgaaaaaac tcgtaatatc ggtatcatgg ctcacgtcga tgccggtaaa
   101641 acaacaacta ctgagcgtat tctttactac actggtaaaa tccacaaaat cggtgaaact
   101701 cacgaaggtg cgtcacaaat ggactggatg gagcaagagc aagaacgtgg tatcacgatc
   101761 acatctgctg cgacgacagc tcaatggaac aaccaccgcg taaacatcat cgacacacca
   101821 ggacacgtgg acttcacaat cgaagtacaa cgttctcttc gtgtattgga tggtgcggtt
   101881 accgttcttg actcacaatc aggtgttgag cctcaaactg aaacagtttg gcgtcaagca
   101941 actgagtacg gagttccacg tatcgtattt gccaacaaaa tggacaaaat cggtgctgac
   102001 ttcctttact ctgtaagcac acttcacgat cgtcttcaag caaatgcaca cccaatccaa
   102061 ttgccaatcg gttctgaaga tgacttccgt ggtatcattg acttgatcaa gatgaaagct
   102121 gaaatctata ctaacgacct tggtacggat atccttgaag aagacatccc agctgaatac
   102181 cttgaccaag ctcaagaata ccgtgaaaaa ttgattgaag cagttgctga aactgacgaa
   102241 gaattgatga tgaaatacct cgaaggtgaa gaaatcacta acgaagaatt gaaagctggt
   102301 atccgtaaag cgactatcaa cgttgaattc ttcccagtat tgtgtggttc agccttcaaa
   102361 aacaaaggtg ttcaattgat gcttgatgcg gttatcgact accttccaag cccacttgac
   102421 atcccagcaa tcaaaggtat taacccagat acagacgctg aagaaattcg tccagcatct
   102481 gacgaagagc catttgcagc tcttgccttc aagatcatga ctgacccatt cgtaggtcgt
   102541 ttgacattct tccgtgttta ctcaggtgtt cttcaatcag gttcatacgt attgaatact
   102601 tctaaaggta aacgtgaacg tatcggacgt atccttcaaa tgcacgctaa cagccgtcaa
   102661 gaaatcgaca ctgtttactc aggtgatatc gctgctgccg ttggtttgaa agatactaca
   102721 actggtgact cattgacaga tgaaaaagct aaaatcatcc ttgagtcaat caacgttcca
   102781 gaaccagtta tccaattgat ggttgagcca aaatctaaag ctgaccaaga caagatgggt
   102841 atcgcccttc aaaaattggc tgaagaagat ccaacattcc gcgttgaaac aaacgttgaa
   102901 actggtgaaa cagttatctc aggtatgggt gaacttcacc ttgacgtcct tgttgatcgt
   102961 atgcgtcgtg agttcaaagt tgaagcgaac gtaggtgctc ctcaagtatc ttaccgtgaa
   103021 acattccgcg cttctactca agcacgtgga ttcttcaaac gtcagtctgg tggtaaaggt
   103081 caattcggtg atgtatggat tgaatttact ccaaacgaag aaggtaaagg attcgaattc
   103141 gaaaacgcaa tcgtcggtgg tgtggttcct cgtgaattta tcccagcggt tgaaaaaggt
   103201 ttggtagaat ctatggctaa cggtgttctt gcaggttacc caatggttga cgttaaagct
   103261 aagctttatg atggttcata tcacgatgtc gactcatctg aaactgcctt caagattgcg
   103321 gcttcacttt cccttaaaga agctgctaaa tcagcacaac cagctatcct tgaaccaatg
   103381 atgcttgtaa caatcactgt tccagaagaa aaccttggtg atgttatggg tcacgtaact
   103441 gctcgtcgtg gacgtgtaga tggtatggaa gcacacggta acagccaaat cgttcgtgct
   103501 tacgttccac ttgctgaaat gttcggttac gcaacagttc ttcgttctgc atctcaagga
   103561 cgtggtacat tcatgatggt atttgaccac tacgaagatg tacctaagtc agtacaagaa
   103621 gaaattatta agaaaaataa aggtgaagac taatccgtcc tcactctaga aggaagtcac
   103681 ttagtggctt ccttttgtct ttagaaaata cctctaaata tggtaaaata gtagaagaat
   103741 aatgtgagga aaatgaatgt caaatagttt tgaaattttg atgaatcaat tggggatgcc
   103801 tgctgaaatg agacaggctc ctgctttagc acaggccaat attgagcgag ttgtggttca
   103861 taaaattagt aaggtatggg agtttcattt cgtattttct aatattttac cgattgaaat
   103921 ctttttagaa ttaaagaaag gtttgagcga agaattttct aagacaggca ataaagctgt
   103981 ttttgaaatt aaggctcggt ctcaagaatt ttcaaatcag ctcttgcagt cctactatag
   104041 ggaggctttc tctgaaggtc catgtgctag tcaaggtttt aagtcccttt atcaaaattt
   104101 gcaagttcgt gctgagggta atcagctatt tattgaagga tctgaagcga ttgataagga
   104161 acattttaag aagaatcatc ttcctaattt agccaaacaa cttgaaaagt ttggttttcc
   104221 aacttttaac tgtcaagtcg agaagaatga tgtcctgacc caagagcagg aagaggcctt
   104281 tcatgctgaa aatgagcaga ttgttcaagc tgccaatgag gaagcgctcc gtgctatgga
   104341 acaactggag cagatggcac ctcctccagc ggaagagaaa ccagcctttg attttcaagc
   104401 gaaaaaagct gcagctaaac ccaagctgga taaggcggag attactccta tgatcgaagt
   104461 gacgacagag gaaaatcgtc tggtatttga aggggttgtt tttgatgtgg agcaaaaagt
   104521 gactagaaca ggtcgtgttt taatcaactt taaaatgacg gactatactt caagtttttc
   104581 tatgcaaaag tgggttaaaa acgaggaaga ggcccagaag tttgacctca tcaagaagaa
   104641 ttcttggctc cgagttcgag ggaatgtgga gatgaataac ttcacacgcg atttgactat
   104701 gaacgtacag gatctgcagg aagttgttca ctatgagcgg aaggatttga tgccagaagg
   104761 tgagcgtcgg gttgagtttc atgctcatac taacatgtcg actatggatg ctttgccaga
   104821 ggtcgaagag attgttgcaa cagctgctaa gtggggacac aaggcggttg ctatcacgga
   104881 ccatgggaat gtccagtcct ttccacatgg ctataaggcg gctaagaaag cgggaatcca
   104941 gctgatctat gggatggaag ccaatatcgt ggaggaccgt gtccctatcg tctataacga
   105001 agtggagatg gacttgtcag aagcaaccta cgtggtcttt gacgtggaaa cgacgggact
   105061 ttcagctatc tataatgact tgattcaggt tgcggcttct aagatgtaca aggggaatgt
   105121 tattgctgaa tttgatgaat ttatcaatcc tgggcatccc ttgtcagcct ttactacaga
   105181 gttaactgga attacagatg atcatgtcaa aaatgccaaa ccactagaac aagttttgca
   105241 agaattccaa gaattttgca aggatacggt cctagttgcc cacaatgcta cctttgacgt
   105301 tggctttatg aatgctaatt atgagcggca tgatcttcca aagattagtc agccagttat
   105361 tgatacgctg gagtttgcta gaaacctcta tcctgagtat aaacgccatg gtttggggcc
   105421 tttgaccaag cgttttggtg tggccttgga acatcaccac atggccaact acgatgcgga
   105481 agcgactggt cgtctgcttt tcatctttat caaagaggta gcagaaaaac atggtgtgac
   105541 cgatttagct agactcaaca ttgatctaat cagtccagat tcttacaaaa aagctcggat
   105601 caagcatgcg accatctatg tcaagaatca ggtaggtcta aaaaatatct ttaagctggt
   105661 ttccttgtct aataccaagt attttgaagg agtgccacgg attccgagaa cggttctaga
   105721 tgcccatcga gagggcttga ttttaggttc agcctgttca gagggtgaag tttttgacgt
   105781 ggtcgtttct caaggtgtgg atgcggcggt tgaggtggcc aagtattatg attttatcga
   105841 ggtcatgcca ccggctatct atgcaccctt gattgccaaa gagcaggtca aggatatgga
   105901 ggaactccag accattatca agagtttgat agaggttgga gaccgccttg gcaagcctgt
   105961 tctggctacg ggaaatgttc actatatcga accggaagaa gagatttatc gtgaaattat
   106021 cgtccgtagt ttgggacagg gtgcgatgat taatcgaact atcggtcatg gtgaacatgc
   106081 ccaaccagca ccacttccaa aggctcattt tcgaacgact aatgagatgt tggatgaatt
   106141 tgcctttttg ggagaggaac tggctcgtaa actggttatt gaaaacacca atgccttggc
   106201 agaaatattt gaatccgttg aagtcgttaa gggtgacttg tatacgcctt tcatcgacaa
   106261 ggctgaagaa acagttgctg agttgaccta taagaaagct tttgagattt atggaaatcc
   106321 gctgccagat attgttgatt tgcggattga aaaagaatta acatccatac tggggaatgg
   106381 atttgctgtg atttatctgg catcgcagat gctggtgcaa cgttctaatg aacggggtta
   106441 tttggttggt tctcgtgggt ctgtcggatc tagtttcgtt gcgaccatga ttgggattac
   106501 ggaggtcaat cctctctctc ctcactatgt ctgtggtcag tgtcagtaca gtgagtttat
   106561 cacagatggt tcgtacggtt caggatttga tatgccccat aaggactgtc caaactgtgg
   106621 tcacaaactc agtaaaaacg gacaggatat tccgtttgag accttccttg gttttgatgg
   106681 ggataaggtt cctgatattg acttgaactt ctcgggagaa gatcagccta gcgcccactt
   106741 ggatgtgcgt gatatctttg gtgaagaata tgccttccgt gcgggaacgg ttggtacggt
   106801 agctgccaag actgcctatg gatttgtcaa gggttacgag cgagattatg gcaagtttta
   106861 tcgtgatgca gaagtagaac gcctcgctca aggagcggcg ggtgtcaagc ggacaacagg
   106921 ccaacacccg gggggaatcg ttgttattcc gaactacatg gatgtctacg attttacgcc
   106981 tgtccagtat ccagcagatg atgtcacggc tgaatggcag accactcact ttaacttcca
   107041 cgatatcgat gagaacgtcc tcaaactcga tgtactggga catgatgatc cgaccatgat
   107101 tcgaaaactt caggatttgt ctggtattga ccctaataaa attcctatgg atgacgaagg
   107161 cgtgatggca ctcttttctg ggactgatgt actaggggta acacctgaac aaattggaac
   107221 gcctacgggt atgttgggga ttccagagtt tggaacaaat ttcgtacgtg gaatggtaga
   107281 cgaaacccat ccgacaacct ttgcggaatt gcttcagctg tctggtctgt cccacggtac
   107341 tgacgtttgg ttggggaatg ctcaggatct gattaagcaa ggaatagcgg acctatcgac
   107401 tgttatcggt tgtcgggacg acatcatggt ttacctcatg catgcgggtc tggaacctaa
   107461 gatggccttt accattatgg aacgggtacg taagggtttg tggctaaaga tttcagaaga
   107521 ggagagaaat ggctatatcg aagccatgaa ggctaataag gtgccagagt ggtatatcga
   107581 atcctgtggg aaaattaagt acatgttccc taaggcccat gcggcagcct acgttatgat
   107641 ggccttgcgt gtagcttact tcaaggttca ccatcctatt tattactact gtgcttactt
   107701 ctccattcgt gctaaggctt ttgatatcaa gaccatgggt gcgggcttgg aggtcatcaa
   107761 gcgcagaatg gaagaaatct ctgaaaaacg gaagaacaat gaagcctcta atgtggaaat
   107821 cgatctctat acaactcttg agattgtcaa tgagatgtgg gaacgaggtt tcaagtttgg
   107881 taaattagat ctctaccgta gtcaggcgac agagttcctc atcgacgggg atacccttat
   107941 cccaccattt gtagcaatgg atggtctggg agagaacgtt gccaagcaac tggtgcgggc
   108001 gcgtgaagag ggagaattcc tctctaaaac agaactacgc aagcgtggtg gactctcatc
   108061 aaccttggtt gaaaagatgg atgagatggg tattcttgga aatatgccag aggataacca
   108121 gttgagtttg tttgatgagt tgttttaaaa aattgcttaa taatctatta aaagaggcta
   108181 acgtatatcc aatagattta cattagcttt cttttttgtt aaaatagtct atggaaagag
   108241 ggtgagagta tgtcaaagat gagtataagc atccgtctgg atagtgaggt taaggagcag
   108301 gcccaacagg tgtttagtaa tctgggaatg gatatgacaa cagctattaa tattttcctt
   108361 cgtcaggcaa ttcaatatca gggattacct tttgatgtta gactagacga aaatcggaag
   108421 ttgctccaag cgttaacgga tttagaccaa aatcgtaata tgagccagtc ttttgaatca
   108481 gtctcagatt tgatggagga cttacgtgct taagattcgt tatcataaac agtttaaaaa
   108541 agattttaag ttggctatga agcgtggttt gaaggcagaa ttattagaag aagttttgaa
   108601 ttttctggtt caagaaaaag aacatcctgc cagatatcgt gatcattcat tgacggcatc
   108661 caagcatttt caaggagttc gtgaatgcca tacccagcca gattggcttt tggtttataa
   108721 agtagacaag tcggaattga ttttaaattt gctgaggaca ggcagtcaca gtgatttatt
   108781 ttaatctatt ttaagggggt tctcatgaaa ctaagaatat ttgcggaaga taagccggct
   108841 aagaaggtat ttgaatatca attagaactt gctgatcgta caattcttct atcgacagca
   108901 ctcttgtcag gtgctattgc tttagcagga atcttttctg ctttgaaaga aaaataaaaa
   108961 tagaaaagag aaaacagaat ggttttacca aattttaaag aaaatctagg aaaatatgcg
   109021 aaattgttgg ttgcgaacgg aattaacgtg caacctggtc acactttggc tctctctatt
   109081 gatgtggagc aacgtgaatt ggcacatcta atcgtgaaag aagcttatgc cttgggtgcg
   109141 catgaggtca tcgttcagtg gacagatgat gtgattaacc gtgagaaatt cctccatgcc
   109201 ccgatggagc gtttggacaa tgtgccagaa tacaagattg ctgagatgaa ctatcttttg
   109261 gagaacaagg ctagccgtct tggggttcgt tcatctgatc caggtgcctt gaacggagtg
   109321 gacgctgaca agctttcagc ttctgctaaa gctatgggac ttgccatgaa gcctatgcgt
   109381 atcgcaactc aatctaacaa ggttagctgg actgtagcag ccgctgctgg acttgagtgg
   109441 gctaagaaag tcttcccaaa tgctgcgagc gacgaagaag cagttgattt cctttgggat
   109501 caaatcttca aaacttgtcg tgtctacgaa gcagatcctg ttaaggcttg ggaggaacat
   109561 gcagccattc tcaagagcaa ggccgatatg cttaataagg agcaattttc agcccttcac
   109621 tacacagcgc caggaacaga tttaacactt ggtttgccaa agaaccacgt ttgggaatca
   109681 gctggtgctg tcaatgcaca gggcgaagaa ttcttgccaa atatgccgac agaagaggtc
   109741 ttcacagcgc ctgacttccg tcgtgcagat ggttatgtca cttctacaaa accgcttagc
   109801 tacaacggaa atatcattga aggcattaag gtgaccttta aggatggaca aatcgtagat
   109861 atcactgctg agaagggtga tcaggttatg aaagaccttg tctttaaaaa tgcgggtgcg
   109921 cgtgccttgg gtgaatgtgc cttggtacca gatccaagtc caatttctca gtcaggcatt
   109981 acctttttta acaccctttt cgatgaaaat gcgtcaaacc acttggctat cggtgcagcc
   110041 tatgcgacta gcgttgttga tggagcggag atgagcgaag aggagcttga agctgcaggg
   110101 cttaaccgtt cagatgttca cgtagacttt atgattggtt ctaaccaaat ggatatcgat
   110161 ggtattcgtg aggatggaac gcgggtacct cttttccgta atgggaattg ggcaaattaa
   110221 ggagataata tgttaggaag tatgttcgtt ggtctcctag tgggattttt agcaggtgct
   110281 atgaccaatc gtggagagcg aatgggatgt tttggaaaaa tgtttctcgg ttggatcgga
   110341 gcctttctag gtcacttgct ctttggaact tgggggccag ttttatcagg aacagctatt
   110401 atcccagcga ttttaggagc catgattgtt ttagctattt tttggagacg aggaagttaa
   110461 tttcctaaat ctgatactca atgaaaatca aagagcaaac taggaaacta gccgcaggtt
   110521 gctcaaagca ctg
//
LOCUS       DBERCA010000002        97696 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.002, whole genome shotgun sequence.
ACCESSION   DBERCA010000002 DBERCA010000000
VERSION     DBERCA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 97696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.002"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(53..379)
                     /locus_tag="ACL719_000118"
     CDS             complement(53..379)
                     /locus_tag="ACL719_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698168.1"
                     /translation="MILWSFDFANDHAHTFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDG
                     FDYEEW"
     gene            complement(706..2586)
                     /locus_tag="ACL719_000119"
     CDS             complement(706..2586)
                     /locus_tag="ACL719_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGS0698169.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2580..3449)
                     /locus_tag="ACL719_000120"
     CDS             complement(2580..3449)
                     /locus_tag="ACL719_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS0698170.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3533..6178)
                     /locus_tag="ACL719_000121"
     CDS             complement(3533..6178)
                     /locus_tag="ACL719_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGS0698171.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPELL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RLYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6269..7549)
                     /locus_tag="ACL719_000122"
     CDS             complement(6269..7549)
                     /locus_tag="ACL719_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGS0698172.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7720..9804
                     /locus_tag="ACL719_000123"
     CDS             7720..9804
                     /locus_tag="ACL719_000123"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGS0698173.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKLGILFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTSFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLTQYQELGYLSTDHNESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9844..11523)
                     /locus_tag="ACL719_000124"
     CDS             complement(9844..11523)
                     /locus_tag="ACL719_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS0698174.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFKKWFETTRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12278..13507
                     /gene="arcA"
                     /locus_tag="ACL719_000125"
     CDS             12278..13507
                     /gene="arcA"
                     /locus_tag="ACL719_000125"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGS0698175.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHDIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13565..14581
                     /gene="argF"
                     /locus_tag="ACL719_000126"
     CDS             13565..14581
                     /gene="argF"
                     /locus_tag="ACL719_000126"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGS0698176.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14746..15693
                     /gene="arcC"
                     /locus_tag="ACL719_000127"
     CDS             14746..15693
                     /gene="arcC"
                     /locus_tag="ACL719_000127"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGS0698177.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15902..17413
                     /locus_tag="ACL719_000128"
     CDS             15902..17413
                     /locus_tag="ACL719_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGS0698178.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17435..18766
                     /locus_tag="ACL719_000129"
     CDS             17435..18766
                     /locus_tag="ACL719_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGS0698179.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGTFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18862..18969
                     /locus_tag="ACL719_000130"
                     /pseudo
     CDS             18862..18969
                     /locus_tag="ACL719_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(19173..19463)
                     /locus_tag="ACL719_000131"
     CDS             complement(19173..19463)
                     /locus_tag="ACL719_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS0698180.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19451..19702)
                     /locus_tag="ACL719_000132"
     CDS             complement(19451..19702)
                     /locus_tag="ACL719_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGS0698181.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(20101..20985)
                     /locus_tag="ACL719_000133"
     CDS             complement(20101..20985)
                     /locus_tag="ACL719_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS0698182.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21130..22281)
                     /locus_tag="ACL719_000134"
     CDS             complement(21130..22281)
                     /locus_tag="ACL719_000134"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGS0698183.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22427..24193)
                     /locus_tag="ACL719_000135"
     CDS             complement(22427..24193)
                     /locus_tag="ACL719_000135"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGS0698184.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24206..24934)
                     /locus_tag="ACL719_000136"
     CDS             complement(24206..24934)
                     /locus_tag="ACL719_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HGS0698185.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIWCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(24949..27966)
                     /locus_tag="ACL719_000137"
     CDS             complement(24949..27966)
                     /locus_tag="ACL719_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HGS0698186.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLTKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            complement(27977..30193)
                     /locus_tag="ACL719_000138"
     CDS             complement(27977..30193)
                     /locus_tag="ACL719_000138"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS0698187.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCVETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            complement(30215..32085)
                     /locus_tag="ACL719_000139"
                     /pseudo
     CDS             complement(30215..32085)
                     /locus_tag="ACL719_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
     gene            complement(32091..33410)
                     /locus_tag="ACL719_000140"
     CDS             complement(32091..33410)
                     /locus_tag="ACL719_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS0698188.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDRYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(33407..34246)
                     /locus_tag="ACL719_000141"
     CDS             complement(33407..34246)
                     /locus_tag="ACL719_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698189.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPIDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(34249..35178)
                     /locus_tag="ACL719_000142"
     CDS             complement(34249..35178)
                     /locus_tag="ACL719_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698190.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            35211..35852
                     /locus_tag="ACL719_000143"
                     /pseudo
     CDS             35211..35852
                     /locus_tag="ACL719_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            complement(36981..38486)
                     /gene="adcA"
                     /locus_tag="ACL719_000144"
     CDS             complement(36981..38486)
                     /gene="adcA"
                     /locus_tag="ACL719_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGS0698191.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(38496..39302)
                     /locus_tag="ACL719_000145"
     CDS             complement(38496..39302)
                     /locus_tag="ACL719_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGS0698192.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(39295..39999)
                     /locus_tag="ACL719_000146"
     CDS             complement(39295..39999)
                     /locus_tag="ACL719_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS0698193.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNVGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(39999..40439)
                     /gene="adcR"
                     /locus_tag="ACL719_000147"
     CDS             complement(39999..40439)
                     /gene="adcR"
                     /locus_tag="ACL719_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGS0698194.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(40594..41862)
                     /gene="dltD"
                     /locus_tag="ACL719_000148"
     CDS             complement(40594..41862)
                     /gene="dltD"
                     /locus_tag="ACL719_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGS0698195.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(41855..42094)
                     /gene="dltC"
                     /locus_tag="ACL719_000149"
     CDS             complement(41855..42094)
                     /gene="dltC"
                     /locus_tag="ACL719_000149"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGS0698196.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(42108..43352)
                     /gene="dltB"
                     /locus_tag="ACL719_000150"
     CDS             complement(42108..43352)
                     /gene="dltB"
                     /locus_tag="ACL719_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGS0698197.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVIT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(43349..44899)
                     /gene="dltA"
                     /locus_tag="ACL719_000151"
     CDS             complement(43349..44899)
                     /gene="dltA"
                     /locus_tag="ACL719_000151"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGS0698198.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(44920..45051)
                     /locus_tag="ACL719_000152"
     CDS             complement(44920..45051)
                     /locus_tag="ACL719_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGS0698199.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(45282..45986)
                     /locus_tag="ACL719_000153"
     CDS             complement(45282..45986)
                     /locus_tag="ACL719_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS0698200.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(46056..47882)
                     /gene="glpO"
                     /locus_tag="ACL719_000154"
     CDS             complement(46056..47882)
                     /gene="glpO"
                     /locus_tag="ACL719_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGS0698201.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(47924..49432)
                     /gene="glpK"
                     /locus_tag="ACL719_000155"
     CDS             complement(47924..49432)
                     /gene="glpK"
                     /locus_tag="ACL719_000155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGS0698202.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(49591..51018)
                     /locus_tag="ACL719_000156"
     CDS             complement(49591..51018)
                     /locus_tag="ACL719_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0698203.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            51183..52055
                     /gene="hslO"
                     /locus_tag="ACL719_000157"
     CDS             51183..52055
                     /gene="hslO"
                     /locus_tag="ACL719_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGS0698204.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            52042..53022
                     /gene="dusB"
                     /locus_tag="ACL719_000158"
     CDS             52042..53022
                     /gene="dusB"
                     /locus_tag="ACL719_000158"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGS0698205.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLID
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(53252..55339)
                     /gene="pspC"
                     /locus_tag="ACL719_000159"
     CDS             complement(53252..55339)
                     /gene="pspC"
                     /locus_tag="ACL719_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HGS0698206.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFLGGVVHAEGVGGRN
                     TSTVTSSGQDTSKKYADEVKSHYQSILEKVRKSLEKDRHTQNVGLITKLSEIKKKYLY
                     ELEVNVLLEEKSKAELPSKAKAELDAAFEQFKKEPELTKKVAEAQKKVEEAKKKAEDQ
                     KEEDFRNYPTNTYKTIELEIAESDVKVKEAELELLKEEAKEHRDEGTIKQVEEKVKSE
                     KAEATRLEEIKTERKKAEEEAKRRADAKEQGKPKGRAKRGVPGEQATPDKKENDAKSS
                     DSSVGEETLPNPSLKPEKKVAEAQKKVEEAKKKAKDQKEEDHRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEAK
                     RKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARR
                     SEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYY
                     LNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQNNGSW
                     YYLNANGAMATGWLQNNGSWYYLNANGDMATGWLQNNGSWYYLNANGSMVTGWLQNNG
                     SWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTV
                     DSYRVNANGEWVN"
     gene            complement(55418..55945)
                     /locus_tag="ACL719_000160"
     CDS             complement(55418..55945)
                     /locus_tag="ACL719_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGS0698207.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(56028..57368)
                     /gene="hk06"
                     /locus_tag="ACL719_000161"
     CDS             complement(56028..57368)
                     /gene="hk06"
                     /locus_tag="ACL719_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGS0698208.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(57365..58018)
                     /gene="rr06"
                     /locus_tag="ACL719_000162"
     CDS             complement(57365..58018)
                     /gene="rr06"
                     /locus_tag="ACL719_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGS0698209.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(58279..60711)
                     /locus_tag="ACL719_000163"
     CDS             complement(58279..60711)
                     /locus_tag="ACL719_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS0698210.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(60713..61171)
                     /locus_tag="ACL719_000164"
     CDS             complement(60713..61171)
                     /locus_tag="ACL719_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGS0698211.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(61290..62018)
                     /locus_tag="ACL719_000165"
     CDS             complement(61290..62018)
                     /locus_tag="ACL719_000165"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698212.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(62018..63025)
                     /locus_tag="ACL719_000166"
     CDS             complement(62018..63025)
                     /locus_tag="ACL719_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0698213.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(63064..63822)
                     /locus_tag="ACL719_000167"
     CDS             complement(63064..63822)
                     /locus_tag="ACL719_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698214.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(63785..64075)
                     /locus_tag="ACL719_000168"
     CDS             complement(63785..64075)
                     /locus_tag="ACL719_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGS0698215.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQGADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(64146..64248)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(64331..65677)
                     /gene="cbpD"
                     /locus_tag="ACL719_000169"
     CDS             complement(64331..65677)
                     /gene="cbpD"
                     /locus_tag="ACL719_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGS0698216.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEMPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGYKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(65766..66032)
                     /locus_tag="ACL719_000170"
     CDS             complement(65766..66032)
                     /locus_tag="ACL719_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGS0698217.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(66034..67386)
                     /gene="dnaB"
                     /locus_tag="ACL719_000171"
     CDS             complement(66034..67386)
                     /gene="dnaB"
                     /locus_tag="ACL719_000171"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGS0698218.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(67430..67882)
                     /gene="rplI"
                     /locus_tag="ACL719_000172"
     CDS             complement(67430..67882)
                     /gene="rplI"
                     /locus_tag="ACL719_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGS0698219.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(67879..69852)
                     /locus_tag="ACL719_000173"
     CDS             complement(67879..69852)
                     /locus_tag="ACL719_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGS0698220.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSKAGEKLTEIVLNEIKEKEKEE"
     gene            complement(69988..70536)
                     /gene="raiA"
                     /locus_tag="ACL719_000174"
     CDS             complement(69988..70536)
                     /gene="raiA"
                     /locus_tag="ACL719_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGS0698221.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(70616..71278)
                     /locus_tag="ACL719_000175"
     CDS             complement(70616..71278)
                     /locus_tag="ACL719_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGS0698222.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(71275..72573)
                     /locus_tag="ACL719_000176"
     CDS             complement(71275..72573)
                     /locus_tag="ACL719_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0698223.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            72629..73264
                     /locus_tag="ACL719_000177"
     CDS             72629..73264
                     /locus_tag="ACL719_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGS0698224.1"
                     /translation="MEFRTIKEDDQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            73549..74469
                     /gene="cysK"
                     /locus_tag="ACL719_000178"
     CDS             73549..74469
                     /gene="cysK"
                     /locus_tag="ACL719_000178"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGS0698225.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(74708..75073)
                     /locus_tag="ACL719_000179"
                     /pseudo
     CDS             complement(74708..75073)
                     /locus_tag="ACL719_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_068988785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            75055..75363
                     /locus_tag="ACL719_000180"
                     /pseudo
     CDS             75055..75363
                     /locus_tag="ACL719_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(75554..75841)
                     /gene="tnpB"
                     /locus_tag="ACL719_000181"
     CDS             complement(75554..75841)
                     /gene="tnpB"
                     /locus_tag="ACL719_000181"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0698226.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(76087..77127)
                     /gene="tsf"
                     /locus_tag="ACL719_000182"
     CDS             complement(76087..77127)
                     /gene="tsf"
                     /locus_tag="ACL719_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGS0698227.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(77206..77985)
                     /gene="rpsB"
                     /locus_tag="ACL719_000183"
     CDS             complement(77206..77985)
                     /gene="rpsB"
                     /locus_tag="ACL719_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGS0698228.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            complement(78209..79387)
                     /gene="pcsB"
                     /locus_tag="ACL719_000184"
     CDS             complement(78209..79387)
                     /gene="pcsB"
                     /locus_tag="ACL719_000184"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGS0698229.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            complement(79481..79975)
                     /gene="mreD"
                     /locus_tag="ACL719_000185"
     CDS             complement(79481..79975)
                     /gene="mreD"
                     /locus_tag="ACL719_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGS0698230.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            complement(79975..80793)
                     /gene="mreC"
                     /locus_tag="ACL719_000186"
     CDS             complement(79975..80793)
                     /gene="mreC"
                     /locus_tag="ACL719_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGS0698231.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            complement(80852..81646)
                     /locus_tag="ACL719_000187"
     CDS             complement(80852..81646)
                     /locus_tag="ACL719_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0698232.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKSMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            complement(81639..82478)
                     /locus_tag="ACL719_000188"
     CDS             complement(81639..82478)
                     /locus_tag="ACL719_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGS0698233.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            complement(82463..83290)
                     /locus_tag="ACL719_000189"
     CDS             complement(82463..83290)
                     /locus_tag="ACL719_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGS0698234.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALDLVGMLDFKKREPARLSGGQKQRVAIAGVVVLRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSHNGYNLPENYLTESELEDKLWELL"
     gene            complement(83287..83832)
                     /gene="pgsA"
                     /locus_tag="ACL719_000190"
     CDS             complement(83287..83832)
                     /gene="pgsA"
                     /locus_tag="ACL719_000190"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGS0698235.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            complement(83843..84664)
                     /gene="rodZ"
                     /locus_tag="ACL719_000191"
     CDS             complement(83843..84664)
                     /gene="rodZ"
                     /locus_tag="ACL719_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGS0698236.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            complement(84706..85989)
                     /locus_tag="ACL719_000192"
     CDS             complement(84706..85989)
                     /locus_tag="ACL719_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS0698237.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLIVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            complement(85986..87236)
                     /locus_tag="ACL719_000193"
                     /pseudo
     CDS             complement(85986..87236)
                     /locus_tag="ACL719_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
     gene            87395..87763
                     /gene="yaaA"
                     /locus_tag="ACL719_000194"
     CDS             87395..87763
                     /gene="yaaA"
                     /locus_tag="ACL719_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGS0698238.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            87766..88863
                     /gene="recF"
                     /locus_tag="ACL719_000195"
     CDS             87766..88863
                     /gene="recF"
                     /locus_tag="ACL719_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGS0698239.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(88914..90392)
                     /gene="guaB"
                     /locus_tag="ACL719_000196"
     CDS             complement(88914..90392)
                     /gene="guaB"
                     /locus_tag="ACL719_000196"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGS0698240.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(90544..91569)
                     /gene="trpS"
                     /locus_tag="ACL719_000197"
     CDS             complement(90544..91569)
                     /gene="trpS"
                     /locus_tag="ACL719_000197"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGS0698241.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            91775..93397
                     /locus_tag="ACL719_000198"
     CDS             91775..93397
                     /locus_tag="ACL719_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698242.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            93459..96011
                     /locus_tag="ACL719_000199"
     CDS             93459..96011
                     /locus_tag="ACL719_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGS0698243.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFNLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(96041..97694)
                     /locus_tag="ACL719_000200"
                     /pseudo
     CDS             complement(96041..97694)
                     /locus_tag="ACL719_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
BASE COUNT        29180 a        21339 c        17095 g        30082 t
ORIGIN      
        1 aaatactaag cctacacaca gactgattcc aattgttttt tagatttcaa ttttaccatt
       61 cttcatagtc aaaaccatca taatctggat attgttctgg cgacgttcca accgaacgta
      121 cgagataagc ttcttcgtcc tctgtctcga tttctctcag cacttggcat tcaaaacgcc
      181 attcatcacc gaagtcgaaa ataaacttaa atttttgttt gacacttagg ctatccagat
      241 agacattttc tgtgtaacgt tcttcaacat cgtcactaac aaagctaaga aagtaagaat
      301 ctgcatgact ccactcaaca ttatccatga aaaatgtgtg agcatgatca tttgcaaaat
      361 caaaactcca taaaatcaca tctgctagtt ccgctaacgt ttttgttttt ggaatctata
      421 tatgtcggta gcaacctttt ttaaatttga cggaaatgat ccaagcttct acagatgctg
      481 gcttttcagc ttctatttct tgttttacta attttaactg tagcatttga aaccagataa
      541 ttcttctgtt tcacgcgaaa caccctgcgc caagcaaggt gttcttgttt ttatactcaa
      601 tgaaaatcaa agagcaaact aggaagctag ctgcaggctg ctcaaaacac tgttttgagg
      661 ttgtagatgg aagctgacgt ggtttgaaga gattttcgaa gagtattaag tcgtataaat
      721 cgtcgacgct gtcgcaatgg tatgccactg gttggctgtt gtagctgcga aatccttgtc
      781 tgcgtagaag tcagtaaatg gtaggatttc aacttccagc tcgtcgatgc ggtcaagctg
      841 gccagccaga taaacctcga tacggctttc tgctcgtttg atgcgttgca agagtccgcc
      901 catacggatg tcaactgtat ccaaaccaaa gaccttgttt tctttcagcc attggtggct
      961 aaagagggca tggaagtctt caatttggct tctaagttct ggtaattctt gtctggcgat
     1021 ttgttgtaaa ctttctttat catccgcttg gtaggcctga cgaatgcgtc gtcccacatc
     1081 tactttgcta cttaaaatag cattcaactg ggcctgagtt tcaaagagat aagcatagtt
     1141 tccagctttt tctttaatgt tagcaagcgt ctcagcagcc tgagcgaagt gtggcttgtc
     1201 ctgttcaggt gtcatgtgtc ggtcaagtat cggacagaga acatcctgat aaaagacata
     1261 tcggttggga ttgatgccac tgagattacc tggtaggtct ggtaaaaggt tggcaaggtc
     1321 aatctgcata aaatcttcaa ccgttagacc agtattggtc ttgaaatgcg cagacaaact
     1381 atctaggtca ttgcgatagc tgagttctgc ccagatttgc aagcttggta ggatagagaa
     1441 ctgggcagtt tcaccaccat tgtctcccca acccgttacg atgacttctt taatctgatt
     1501 ggcacggcag gctttattag cctcgatagc cactagacgg ctaaaatggt tgtgaggtgt
     1561 aaagccaatc cacttccaag ctccccctgc aaatgcaagg tcatggctaa tcttgtgatg
     1621 attgcggaaa ttacggttgt atttttcctc gctatcctga taataatccc agtaaaccag
     1681 agtcacacgg tctttgagac ggtctaggta gacacgagtt tcctctggaa tttccacatc
     1741 acggtcgtac tggccatccg ctgacatgag tttgaagaac atatcactcc acatctggca
     1801 gtggaaacca tatttgtcag caatatccag cacgcgctcc aagtgttggc acatgaggag
     1861 actacgatcc acaacaccgt tcaaaatcag gtagcgtccc aaaccaacca agtgggcttc
     1921 gtccatcccg atattgacct tgcgagtctt cagtttagac aacgtggcaa acatgccatc
     1981 aatcaggtca taaacctttt cttcgccaat gagaagaatg tcctctacat cacggagctc
     2041 ctgcacttcc ttgacacccc atttgacaaa ggccgacaag tgggccaagg tctggatgca
     2101 tggtacaaag gtcatgtcaa actgttgagc ataggcttcg atttcctgca actcctctgc
     2161 tgaataagct ccacggaaat agccaaagta aggctgccct tcaatctggt aagtgtcttc
     2221 catgtaaagt tcaaaggttg agtagcccat gagagccaag acctcaatca tctgcttggc
     2281 agaagccaca ttcagcaccg catttcgaga acagtcaacc atgtaagcca aatcttcgta
     2341 agctgcttgt tcctcaatct ctactttatc agcttctgct agaactgtta ccaacaagga
     2401 taaggcacga taaagttggt gaggtttgcg gtaggtcaat tgatagtgac cttcctcacc
     2461 ctcgatagag atagaggctt ggtcagactg agtgacagcc acttccacat ctggtagaga
     2521 aatgtgacct tttaaaacct caatagcttg cgtttgtttg ggactaagtc ctgtaaatct
     2581 taccattgct tttcctcctg tagccagttg acaagggcac cgtagagatt ggcatctgcg
     2641 tgataggtgc aggtctggat aactggtgcg accgtgtatt cttcgtaggc atcgacaaag
     2701 tcttcaacag ccttcttaac accttggata aaatctggat tttgactgat agagccaccc
     2761 agactgatga catctggatc gatcagatac tggatattga gcaagccttg cgtcagattg
     2821 cggttcatgc gctcaatggc ttcttgacaa agagcattac cagctgcggc ctcttggtaa
     2881 atcttgcgac cgtcccaatc agtatgacca gatttttcaa tcacgtatcg taccatattc
     2941 ccagttgatg ctagttgcga ccagttatta agtttttcag caggggcaag ggttgtcatg
     3001 tagccaaatt ctccacccag accgtggcga cctcgatgaa gtctaccatt gataatcatg
     3061 gctccgccaa tccctgtccc aatcacgaca caggctgcat tttcaagctc tggatgagct
     3121 agtagttcac tgagtccaac gcagttggca tcattttcta aatggacagg tagctgataa
     3181 gagctaagcg cctcatacca agaaaagcca tggatgtagg gcaccgcact gaagccatca
     3241 atcacacctg tctcttgatt gaccgcacct ggaacgctca tagcaatccc actgtaatcc
     3301 tgttctgaca agcgttgatc tagccacgct agtaaatcct ccaagttttc aggcgttgaa
     3361 atacttgtct tatccagtat tttcccatca ggagtcagac tggcaaactt aatcccagtc
     3421 cctccgatat caatcgttgc aatggtcatt gtttttacca taaaaagggg cgaatcagca
     3481 gttagttctt actttttcgc ccatcctttc ttttgatgtt tactttttga aattaaattt
     3541 cttctttttt gatgaattct gtacgaatct cttgtggagc caatagtcct gaatgaactg
     3601 gatatagtcg ctcaagtaag tcaagaatgg tttgttgatg ttctgatatg cgcacatttt
     3661 cttgactcat attatagtaa cggaggacat agccttcttc attttccgcc actttaaagg
     3721 ctgttggaca aacttgcggt atgctgagaa cagaatggct caagaggcta ccagtcgcaa
     3781 ccacgcttcc ttcctgtcta gcaagctgaa ggctggtaaa cggtgtctgc aaggctttgg
     3841 cacgacgata ggctgagaag cgttcttggg cttggtggca ttcaagtgca aactcgactt
     3901 caaactcccg caagcattgt gcttctggcg ttgggaagta gccccagtca cctagctcac
     3961 ctgatgcacg caaaatggtc acggcaatgg tgttatcccc aaggatttcg tattcattca
     4021 atcccttgtt ggatacagtc accccttttt catcgtcata cagactgaca aaagcttgtt
     4081 ggtgttgagg attttcaggg ttttcccatg aagcagctgg tttgtttggt cgtgtcacca
     4141 cctcatagat actttcagaa tcattgcttg gacgcgtgtt atgagtcttg accaagagac
     4201 ggatacggtg atccttggca gtgttagtaa agcgagtctt gaagcggatt tgtggattgt
     4261 caacgaagac agtcaactca gtttccagag gaatgtttgt caattcttct gaccgtccag
     4321 cctcacgctt cataaactcg atgatacctt gttgctcttc ttctagcttt tcatccgcac
     4381 tgacaggcac ggtcaattca tgtttgagca agattttagc atagcgatct gtgttttcca
     4441 agacctcgtg gtccttaagc tctgcaaaga ttggctctgt tccttttggt tggaaataga
     4501 tatactcatt tccgatgtcc ccacggtctt caaagcggat aaagtcttca taggcttcgt
     4561 gagttgtctt atcatagact gtgatgttgt catccacact caccgttacg aatggtgtat
     4621 caatcactcc gttttggtaa ataccctcac ggtgttcttg ttctccttcc agcaattgga
     4681 aggttgtcca agaaagcggt gctaggtgaa ctggaatggt cacgcgcact tgtcgagcaa
     4741 tacgagcttg gcggaacttg tcttttggta aatcgtattc aaaattagct ccgaggtctt
     4801 cgattgtagc ctctacagga cgaccatcca agtcctccac acggtaactt ggcaagataa
     4861 gagcagccat ctttttgtag ccttctgttg ggtgcaattc cttgaaatca caagtcgcca
     4921 catcaatcac tgtgctgaca gtatcgacct tatcatgcaa gcctgtgtta atgacagtaa
     4981 agagatagtc actttgagcc ttatccgtag caattttacc cttccactcg ttgagcaaat
     5041 tacttttaac aaagtttcct acttggttga ccttggcaaa acgcgtttcc atctcgcggt
     5101 gaacttcgtc cacgctacag ccacagatac tatcatgtgg cgcattctgc aaaagtgttt
     5161 tccaagcata ggtcaactgg tccttgtggt tgtgtccacc agtgataata gtcaagggtt
     5221 ctaccacttg ctctaggagg ttgctatttt cttggaaggc ttgttttagg taaatgcggg
     5281 atgaagaagt gttggcaagt gtgtaccagc catctgtttc ctgactggtc aactcacctg
     5341 taacagttga taagagttca ggaagcgcac cttctacagc ttgaacatat tcatcaaaag
     5401 aactatgaac aaagattaca tccgggaaga gttcatttgc cacacgaatg gcttcgctca
     5461 gatttttctg tacaggctgg tggtcacagc cgttcatcat caaccattgg ttggtcgaag
     5521 cgtaggcacg cacatctgac aatttttgtt tccagaaggt caaggcctca tctttgtcaa
     5581 ctgggatttc attcccatta ctgtaccagt tagcaaagag aatacctaaa acacgactac
     5641 catccacacc ctgccagtac atttctgaaa actgggatgt aaactgctca tcttcaagga
     5701 cttggttgtc aaatccaatc ggcttcacac cacgaccaaa ggccgccacg tgaatgcctg
     5761 atttttgaag aatttgaggc gcttgtccca tatttccaaa ggtatctgga aagtagccaa
     5821 tctgggttga tttaccccat ttggcagctt cttgttgacc aatcaaggta ttgcggacat
     5881 tggcttcact ggagatcaag tagtcatcct gcaagatgta aaagggacca attttaagtt
     5941 tgccctcgtc aatgtagcgt tggaccttgt cgcgattttc agggcgaatt tgtaagtagt
     6001 cgtcaaggac aatagtttgt ccatccaagt ggaaactctt gaactcaggg tcattttcaa
     6061 agagatcaaa gagattgtca aacaattcca ccaactgcat acgatggctt tcaaaaggca
     6121 agtaccactc acggtcccag tgactatgtg agataatatg tacaacaaca ttttccatga
     6181 agtaaaacct cattctaaat ttaaatttta acgttttaaa tgtaaacttg tgattctgat
     6241 aagaatcggt tgagctaaag cgagcccctt agcgaatatc caagtaatcc aagaccaact
     6301 cacagaacat catgttagcc caggagaacc attcacgaga gtagagggtc ggatcatcta
     6361 cgtggaagct ttcgtgcatg acacctgtac caccatcgca ggcaaccagc tgatccagca
     6421 agaatttttt ctctgcctta tctcttgttg tcaatccttg gatagaaagg gcgattggcc
     6481 agatatagcg atagaaggta tgagaacttc cgagaccgct agcgtattct ccttggtaga
     6541 agtatggatt ttcagagctc aaaatggtac ggcgagtagc ttgatacact tcatcatcga
     6601 ccgaacagta gcccagatag ggcgcagcta gtagacttgg tacatttgga tcatccatga
     6661 tgctggcatt tcctaggcca tccacttcaa aagcgtaaat cttttcgccc ttgctgttgg
     6721 tggtgtaagc gtagttttcg attccttctt ggatttcatc ctgaagacgc ttggcatcag
     6781 caataacact ctggctatca gctaggttta atgctgcgaa gatttcttgc acataaccca
     6841 agactactac agcaaacata tttgacggaa tcaagtaact atactggcaa cagtcatcac
     6901 tcggacgaaa agctgaccaa gtcatacctg tcactgcaaa gtcaggtcca aagccatcat
     6961 ttaccaaggt gtcttcctta cggtccgtat ctcggacaaa acgataagga gagttcttgt
     7021 ggtcttgttc aactgtccat agatggagaa tttccttagt cgctacgaca aaaatctcat
     7081 caaactgact agtctcgcca gtctctttcc agaggagata agccaactgc aaaggatagc
     7141 aaagtgaatc cacctcatac ttgcgctccc agatccagcc gttaaggtct gtgtgatcag
     7201 tctcgtggtg ccctttccag ttctcctcaa tgttgaagga gttagcatag ggatccttga
     7261 gtaccaaggt catctgacgt ttgaccaaac ctgcaatggt ctgacgcagg agggcatctc
     7321 ttttagctac atgaaggtag ggtctgagtt gggctgtcga atctcgaagc cacatggcag
     7381 gaatatcccc agtcaagaca aaagttgaac catcttctaa gatttcaacc gtattgtcca
     7441 aggtatcggt gtagcaacgc tcgaaaacat ccacccactc tggatggtcc ttagcccgct
     7501 ctgctacttc atctagccat tctctaacaa tttctttcga ataaatcatc gtgcaattcc
     7561 ctctttcaaa caagtttcat tttcagtata tagcttttga gacagaaaat acatacacaa
     7621 atgatagtca tttttctaca aaatagttat ctttaaaact ccttttctaa aggccctctt
     7681 tttctgatat aatgtagaaa aacagctaaa ggaaagacta tgaaaccact acttgaaacc
     7741 atcgataccc gctttggtac tgctagcaag catgccttct ctcggggaaa taccctgcca
     7801 tacacaggcg tgccttttgg gatgaattat tttgtgcccc agaccagtga ccaggacgga
     7861 gcttggtttt ttgatccgca tctgcctatc tttcagggga ttcgattaac tcaccagccc
     7921 agtccttgga ttggggacta ctcttggctc cttctgacac ctgtcaccag tcaactgggt
     7981 ggagacagcc tctttcaccg ccagtcttct tatgatatag ataaagcttg cttccagcct
     8041 cactatttga agctcttttc cctgcgctat caaattgaaa cccagctcac accgacttgc
     8101 tatggtgctt ctattcgttt gaatcaaaag caaggcaaag ccctctccct ctatcttcac
     8161 gcagcagatg aactgaccgt agagcaagta gataagcgga ctctggctct cagacaagaa
     8221 ggcaaaacag agaccaataa aaatccactg atgctcttca ctgccctgca aatgaacacg
     8281 gatattcttg ctatcagcca agaagctgga gactggcgaa ttgacttagc aagtagtcaa
     8341 accgagatgc aactagccac ttctttcatc tctccttctc aagcactact taatctacct
     8401 caagaagatt ttgatagctg taaatcaagt gcccaagcgg attgggaaaa tctcctccat
     8461 cgttttgaca ttatagagat aggagaggct gaccgaacct tctttgacca ctgtctctac
     8521 agactcttcc tcttcccaca gactttttat gaggttgatg agtcagggca agccatccac
     8581 atggatctgg ctactggtac tgtcaagctt ggtatcctct ttagcaacaa tggtttctgg
     8641 gataccttcc gcaccacctt ccccctcttt gcccttatca taccggagca ctatcaacgc
     8701 tttttagaag gtttcctcaa tagctaccgc gatactagtt tccttccaaa atggctggct
     8761 ccagatgaac ggggtatgat gccaggtaca cttttagacg gtattatcgc agatagcgcc
     8821 tgcaaggaca tggcccccga cctagaagga gaactcttcc aagccatgct cgaaacagcc
     8881 agcaaagccg accctctcgg catcaatggc cgccacggac taacccaata ccaagaacta
     8941 ggctacctct ctaccgacca caacgaaagt gttagccata ctctagacta tgcctatagt
     9001 gatttttgta tcgccagctg tgccaaaaaa ctagagaaca tagaaatcgc tgaaacctac
     9061 aaggctgctt cacaaaatta ccgccagcta tttgacgctg agacaggtta catgcgagca
     9121 cgagacaatc aaggaaactt tcaccctgac ttctctcctt atagttgggg gcgagactac
     9181 gctgaatgct ctgccattca agctacttta ggcgttctcc acgacatccc tggcttaatc
     9241 caacttatgg gtggaaaaga aacctttagc aactatcttt tgaaagcctg tcaagatgct
     9301 cccctctttg aaacaacagg ctatggttac gaaattcacg aaatgagcga gatggctact
     9361 gctccttttg gacaaatagc catttccaac cagcctagtt tccacattcc ttatctcttc
     9421 cgctacagcg attaccctga ctacactgcc cttcttatca agacgctccg tcagaaagct
     9481 tttcacccaa gttgggaagc ctatcctgga gatgaagaca atggtagtct ctctgcttgg
     9541 tacatctggt cagctctcgg attttatcca acctgtccag gaaaaccaag ctatgacctc
     9601 ggaatccctc tctttgacca tctacgagtc tacctcgcta aagaagataa atggctggat
     9661 atccatacta aacaaaacca caaccatttt aactttgtca aagaatgccg actggacaaa
     9721 acactcgtat caactattca acaccaagac ctcttaaaag ctgaacaact aactttcacc
     9781 ctcagctggt taccaagtca ctaataacca aaagaacctt tgcatcgcgc aaaggttctt
     9841 cttttatgaa acttgaactt gaagttgatc cacaagctga ccatccaccg ttaggttcag
     9901 atagaaatcc aaggttttat ctcctgcgaa atacaaggtt ggtagcgtca gacttttttc
     9961 agtctcacca gcttgaaacg caagaacttg aatctcatcc tgataggcga caccatggac
    10021 acctgtcccc ggttgaatcg aaatcttagc ctctaaagag gagctacttt cactacgttc
    10081 aatcctaaaa tgaagagtct ctccctttgc cacagctagg cttttttctg caaatgctag
    10141 ttcctgaaca acttcttttt ttgataaccc aggagtttta taaagggaaa tcttggtcag
    10201 tagaggcata gattgtgctt cggtaatcat cacgcgcacc ttctgtgcct ctactagcgg
    10261 acctcgcaag aggcgcttat gaccaactgt gaagcccctg ccaaattcct gccagacacc
    10321 atccacctct acttgcacat ggaaagcagc gattcgttgc cctagcttca aatcttctct
    10381 taactcaatt acatcaaaag ttttaggaga acctaagtcg agttctaact ggatgggcaa
    10441 ggctgcatcg tttgcccaag agctggtctc aaggccgtct gtcaaatggc gacaagcaaa
    10501 gtctgcggaa agagctggac cagatacctc agctcccaga gccaaatctt ctttatagag
    10561 ctcattgcga taggtcgcaa attcataaag tcgttcaata tcctttgcat caaagagccc
    10621 agcttgattc ggcggaatat taagcaagag tggagttcct cgccctactg agtgaaagta
    10681 gatttcgacc aactcctcga gagacttagg atcctgatcc tcatggtaga accagcctgg
    10741 acggatggaa acatctgcct ctccgattga aaaaatcgtg cccgagggat ccccgtgctg
    10801 aagatagttc agctctgctt ctgttcctag tttatcagga ttcacctttt gccacagtgg
    10861 atcacctgca taccctcgtt cattgccaat ccagcggata ctggtgcctt ctgttgaaaa
    10921 aatcaagcaa tcgccctgca ggtcacgagt ggtttcaaac cattttttaa attcataatt
    10981 aaccttttgc gcaccctctc ctctggcacc atccatccaa acctcagcga acttaccagc
    11041 attcccatag ttaggatttg ataagatttc cttcaactga gccagataat aggcattgta
    11101 gtccgcttct cggtccacat gatagagggg actatgggca tcccacggtg acaggtagac
    11161 ccccatatcc atatcaaact ctgtggcagc ttgggatact tcaaggagca agtcgccctt
    11221 tcctctcctc caaggactga ccttaaccga ataatctgtg tgagctgtcg gataaaggac
    11281 aaagccatcg tggtgcttga ccaccaaaat caactttttg aagcccgttt ccttgagcac
    11341 acgaacccac tcacgcgcat ccaactgact cgggttaaag cgctcaggat cctcctgtcc
    11401 agtcccccat tcttggtcat aaaaggtatt aggaccgaag tggataaaag ctgctagttc
    11461 atctcccaga taagctagct gagtctgact tggtaacggt ccatgcggtt tgattttctt
    11521 catcgttata tatctccttc tttagcaact tctcttgttc acaaggttat ccacaggttg
    11581 tggataataa accatgatct aacagccagc tctttccaac tccaactggt aggctcagga
    11641 tttttctcct caatattccc tcttcaaaca gctctgtcat atggttctgc taacacctct
    11701 cagaatgcta ctctccataa ttattctcag gcttctttca ggtcattgtg tgaagaccat
    11761 tcactctcca gacttttcca cagattgtga ataagtgatt attctaagct atcacctcgt
    11821 cgactataac aaaaaataaa aagagggtca ataagataat tatctcttcc taaatactaa
    11881 gaaaagattc tataatttgt acaattgaac aagaaatgct tctccaaaat ctatcttcaa
    11941 aagaatgttg catcttttta tcctttcaca caaacatttc tctttataca gatccaagtg
    12001 ataaccagat gataattgga cactttttgg tcatttgtat attttttgaa aaatttttta
    12061 aaaaaatttt atatcctttt tatagaagtt tatgcatttg tatctatgta ttctatccta
    12121 tgttatgtat aataacataa aattgtgcta aacatcataa tttatattat tttccgagaa
    12181 attatagcca ttgacattac ctaggaattg cgttataaca tacatgtaag cggtacccaa
    12241 aatggtgcca agtcaaaatt tttaaggagg aaaatacatg tcttctcatc caattcaggt
    12301 cttctcagaa attgggaaac tgaaaaaagt tatgttgcac cgtccaggca aggagttaga
    12361 aaacttgttg ccggactatc ttgaaaggct tctttttgat gatattcctt tcttggaaga
    12421 tgctcaaaaa gaacatgatg catttgccca agctcttcgc gatgaaggaa ttgaggttct
    12481 ctacctagaa caactcgctg ctgaatcatt gacctctcca gaaatccgcg atcaatttat
    12541 cgaggaatac ttagacgaag ccaacatccg tgatcgtcaa accaaggttg ctattcgtga
    12601 attgcttcac gacatcaagg acaaccaaga attggttgaa aaaacaatgg ctgggattca
    12661 aaaagttgaa ttgccagaaa ttcctgacga agctaaagat ctaactgact tagttgaatc
    12721 agattatcca tttgcaattg acccgatgcc aaacctctat ttcactcgcg acccatttgc
    12781 aacaattgga aacgccgtat cgcttaacca catgtttgca gacactcgta accgtgaaac
    12841 actctacggt aagtatatct tcaaatacca cccaatctat ggcggaaaag tggatttggt
    12901 ctacaaccgt gaagaagata cgcgtatcga aggtggagac gagctagttc tttctaaaga
    12961 cgtccttgca gtaggtatct ctcaacgtac agacgcagct tctatcgaaa aacttttggt
    13021 caacatcttc aagaaaaatg ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa
    13081 ccgtaaattc atgcacttgg atactgtctt cactatggta gactatgaca agttcactat
    13141 tcacccagaa atcgaaggcg accttcacgt ttactcagtt acttacgaaa acgaaaaact
    13201 taaaatcgtt gaagagaaag gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga
    13261 aaaagttcat ttgattcgtt gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg
    13321 gaacgacggt tctaacactt tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa
    13381 taccgtgacc aataagattt tggaagaata cgggcttcgc ttgattaaga ttcgcggaag
    13441 tgaattggtt cggggccgtg gtggacctcg ttgtatgtct atgccatttg aacgtgaaga
    13501 agtgtaatcg ctgttcggca ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa
    13561 taaaatgaca aattcagtat tccaaggacg cagcttctta gcagaaaaag actttacccg
    13621 tgcagagtta gaatacctta ttggtctttc agctcacttg aaagatttga aaaaacgcaa
    13681 tattcaacac cactaccttg ctggcaagaa tatcgctctc ctatttgaaa aaacatctac
    13741 tcgtactcgt gcagccttta caactgcggc tatcgacctt ggtgctcacc cagaatacct
    13801 cggagcaaat gatattcagt tgggtaaaaa agaatctact gaagatactg ctaaagtatt
    13861 gggacgtatg tttgacggga ttgaattccg cggattcagc caacgtatgg ttgaagaatt
    13921 ggcagaattc tcaggcgttc cagtatggaa cggtctaact gacgaatggc acccaactca
    13981 aatgctcgct gactacttga ctgttcaaga aaacttcggt cgcttggaag gcttgacatt
    14041 ggtatactgt ggtgatggac gtaacaacgt tgccaacagc ttgctcgtaa caggtgctat
    14101 ccttggtgtc aatgttcaca tcttctcacc aaaagaactc ttcccagaaa aagaaatcgt
    14161 tgaattggca gaaggatttg ctaaagaaag tggcgcacat gttctcatca ctgaagatgc
    14221 tgatgaagca gttaaagatg cagatgttct ttacacagac gtttgggtat caatgggtga
    14281 agaagacaaa ttcgcagaac gtgtagctct tcttaaacct taccaagtca atatggactt
    14341 agttaaaaaa gcaggcaatg aaaacttgat cttcctacac tgcttgccag cattccacga
    14401 tactcacact gtttatggta aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt
    14461 aacagacgaa gtcttccgca gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat
    14521 gcacactatc aaagctgtta tggctgctac acttggtaac ctttatattc ctaaagtata
    14581 attttagata ataaaccgtc taccaacagc tatgagggct gcgactaata gctttagtcc
    14641 ggtcctcttt tatgtaatgg taatctatta tttcttataa aatatgtgaa aaatcattaa
    14701 attgaaatct aaacgcattc tattgagtgt gataaaggag aatttatggc aaatcgtaaa
    14761 attgtagtag ctttgggagg aaatgcgatt ctttcttctg acccatcagc aaaggctcaa
    14821 caagaagctt tagttgaaac agctaagcat cttgtaaaat tgattaaaaa tggagatgat
    14881 ctgattatca ctcacggtaa tggacctcaa gttgggaatc tcttgctcca acatttggca
    14941 tcagactctg aaaagaaccc tgccttccca ctcgactcac ttgtcgctat gacagaaggt
    15001 agcatcggtt tctggttgaa aaatgctttg caaaatgctc tcttggatga aggcatcgaa
    15061 aaaaatgttg cctctgttgt aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt
    15121 aacttgagta aaccaatcgg tcctttctat tcagaagaag aagcaaaagc agaagccgaa
    15181 aaaagcggag cgactttcaa ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca
    15241 ccaaaacctg ttgacatcaa agaaattgaa accatccgta ctcttttaaa taatggtcaa
    15301 gtcgtcgtag ctgcaggtgg tggcggtatt cccgtcgtca aagaaaacaa tggacatttg
    15361 actggtgtcg aagcggttat tgataaagac ttcgcttccc aacgtttggc agaattggtt
    15421 gatgcagacc tcttcatcat tttgacaggt gtagattatg tatttgttaa ctacaacaag
    15481 ccaaaccagg aaaaattgga acatgtgaat gttgcccagc tggaagaata tatcaaacaa
    15541 gatcagtttg caccaggtag catgcttcca aaagtagaag cagctatcgc ttttgtcaat
    15601 ggtcgtccag aaggaaaagc agttattact tcccttgaaa atctaggcgc cttgattgaa
    15661 tctgaaagcg gaacaattat tgaaaaagga taagttgttt tactaataag atgtattcta
    15721 tttctagtat ctttatatca aattagaaat tattcttgaa aacatgtaca atatttcaaa
    15781 agatactagt tttagacttt aatatggtaa aacaaatata aatagaaagc gttttcttga
    15841 atgtttattt aagaaagtag ttggtttttt acactttgtt agacatcagg aggaaaaaca
    15901 aatgagtgaa aaagctaaaa aagggtttaa gatgccttca tcttacaccg tattattgat
    15961 aatcattgct attatggcag tgctaacttg gtttatccct gcgggggcct ttatagaagg
    16021 tatttacgag gctcagcctc aaaatccaca agggatttgg gatgtcctca tggcaccgat
    16081 tcgggctatg ctaggtactc atccagagga aggttcgctc attaaagaaa cgagcgcagc
    16141 gattgatgta gccttcttca tccttatggt tggtggtttc cttggcattg tcaacaaaac
    16201 tggtgctctt gacgtaggga ttgcctctat cgtgaagaag tataagggcc gcgaaaaaat
    16261 gttaattttg gtactgatgc ctttgtttgc cctcggtggt acaacttatg gtatggggga
    16321 agaaacaatg gccttctatc cactccttgt gccagttatg atggccgttg gttttgatag
    16381 cctgactggt gttgcaatta ttttgctcgg ttctcaaatc ggctgtttgg catctactct
    16441 gaatccattt gcgacaggta ttgcttcagc gactgcggga gttggtacag gggacggtat
    16501 cgtacttcgt ctgatcttct gggttacctt gactgctctt agtacttggt ttgtttaccg
    16561 ttatgcggat aagattcaaa aagatccgac taagtcactg gtttatagta ctcgcaaaga
    16621 agatttgaaa cactttaacg tagaagaatc ttcatctgta gaatctacac ttagcagcaa
    16681 acaaaaatca gttctcttct tatttgtgtt gacattcatc ttgatggtat tgagcttcat
    16741 tccatggaca gaccttggcg ttaccatttt tgatgacttt aatgcttggt tgactggtct
    16801 tccagttatt ggtaatattg tcggttcatc tacttctgca ctaggtactt ggtacttccc
    16861 agaaggcgca atgctctttg cctttatggg tatcctgatt ggtgttattt atggtcttaa
    16921 agaagataag attatctctt ccttcatgaa tggtgctgct gacttgctca gtgttgcctt
    16981 gattgtagcg attgctcgtg gtattcaagt tatcatgaac gacggtatga ttaccgatac
    17041 aatcctcaac tggggtaaag aaggcttgag cggtctatct tcacaagtct ttatcgttgt
    17101 aacttatatc ttctatctac ctatgtcatt cttgatccca tcttcatctg gtcttgccag
    17161 cgcaactatg ggtatcatgg ctccacttgg agaatttgta aatgtccgtc ctagcttgat
    17221 tatcactgct taccaatctg cttcaggtgt cttgaacttg attgcaccaa catctggtat
    17281 tgtgatggga gctcttgcac ttggacgtat caacattggt acttggtgga aattcatggg
    17341 caaactcgta gtcgctatta ttgtagtgac catcgccctt cttctccttg gaaccttcct
    17401 tccattccta taaaatagtg agtgaggtga ttccatgaaa atagatataa caaatcaagt
    17461 taaagatgaa tttcttatat cattaaaaac cttgatttcc tatccttcag tactcaatga
    17521 aggagaaaat ggaacacctt ttggacaagc aatccaagat gtcctagaaa aaactttaga
    17581 gatttgtcga gacataggtt tcactaccta tcttgaccct aaaggttatt acggatatgc
    17641 agaaatcggt cagggagcag agcttctggc cattctctgt catttggatg ttgttccatc
    17701 aggtgatgaa gcagattggc agacaccgcc atttgaagca actatcaaag acggctgggt
    17761 attcggacgt ggtgtccaag atgataaagg cccttcgctc gcagctctct atgcagtaaa
    17821 aagcttgctg gaccaaggta ttcagttcaa aaagcgcgta cgctttatct ttggtaccga
    17881 tgaggaaacc ctctggcgct gcatggcacg ctacaatacc atcgaagaac aggccagcat
    17941 gggctttgca cctgactcat cttttcctct gacctatgct gaaaaagggc ttctacaggt
    18001 caaacttcat ggccctggat cggatcaact agagcttgaa gtaggaggca cctttaacgt
    18061 tgtaccagac aaggccaact accaaggtcc cctctatgaa caggtttgta acgatctcaa
    18121 agaagctggt tatgattacc aatccactga acaaaccgta acggttctcg gagtgccaaa
    18181 gcatgctaag gatgctagtc aaggtatcaa tgctgtcatc cgactagcta ccattcttgc
    18241 tcctctccaa gaacaccctg ctctcagctt tcttgcaaca caagcaggtc aagacggcac
    18301 aggaagacaa atctttggtg atatagcaga tgaaccttct ggtcacctat cctttaatgt
    18361 cgcaggtctc atgatcaatc atgaacgttc tgaaatccgt attgacattc ggactcctgt
    18421 cttagctgat aaggaagaac tagtagagtt gcttacaaga tgtgcacaaa actaccaact
    18481 ccgctacgaa gagtttgact atctagcgcc tctatacgtc gcaaaagaca gtaaactcgt
    18541 tagcacactg atgcaaatct accaagaaaa gactggcgat aacagtcctg ctatttcatc
    18601 cggtggtgcc acttttgctc gcaccatgcc aaattgtgta gccttcggcg ccttattccc
    18661 aggagcgaag cagacagaac atcaggcaaa tgaatgtgcc gttctagaag atttgtaccg
    18721 tgctatggat atttatgccg aagccgtcta tcgacttgca acttaatcag gcaactgttt
    18781 ctaccaaaaa aaaatcgacc gattaatgaa ctgcacccca aaagttagac agaataaatc
    18841 taacttttgg ggtgttttat tatgaaattg agttatgaag ataaagttca gatctatgaa
    18901 ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct
    18961 ggtctaaagt catctgaatc tttgagatga gctttataaa tcgctttttt cagtttttgc
    19021 actggtgttt cgataaactc aaacttttta gccgtggtat tgcctgattt tatagtatat
    19081 tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat ttgactgtcc
    19141 tgatcgattt gtcctgttct tatttcattt tactatattt gagccacttc gtctttaacg
    19201 gctttattca taagctcttg taatttttct ttactatcaa ttacttctga ttttccgttg
    19261 taatttattg taataggttt taacttacct aatttctcga cacgctcatt aatttgatct
    19321 tttttgaagg ctgcatatgt ttttcctaag attttttcga aaatatattt atcagatagc
    19381 ggtttgcctt cttcttcagc ttggtttttg tattgatttg aaacataagg aacaaatcct
    19441 tcatagtaac ctaatgctcc cataagttca aaagcttgtt ttctaattca aaccattgca
    19501 actcagattt cagcttttca gataaatcct gctcatccaa ataatgactt gaaattagtg
    19561 ctgaactcgt ttctgtatcc tgtacaggct gatcacccat accagcaaaa aataaactcg
    19621 ttcctagcaa gaccgaacaa gctcctattg catatttcct caaagaaaaa cgctgctttc
    19681 tctcaaattg aaattctttc atcccatctc ccatcattca ttattactgt atattttgta
    19741 tatcagaaat agtttgtatt cacaaatctt tctagttatt cccttatcat tcctaattaa
    19801 gggagataac atacaataat ttttagttaa atgtatatcg atgttttttg tttttcttaa
    19861 taaacgcaat acaaaaagag cctgttgcca agctctttgt actcaatgaa aatcaaagag
    19921 caaactagga aactagccac aggttgctca aaacaccgtt ttgaggtagt agataagact
    19981 gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac
    20041 atctatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagtcta
    20101 ttatttcttc tcagcgcgaa gggctgacaa gatttgtgtt cggatatcat ccacaccatt
    20161 tggagtattt ggtaaaaaga tagtttgatt tcctttagag gcaaaggtat tcaaggtatc
    20221 caaatactgg ttggtcaaga ggatagacat gatttgttct tctgtcatgc caacattggc
    20281 ttccttgagt tcggtgatag actctgccaa tccatccaca atcgccttac gttgttgggc
    20341 aatccccaca ccatgaaggc ggtctttttc tgcttctgct tcagctgcag tgacaatttt
    20401 aatcttgtca gcttccgcca attcttgtgc tgcgacccgc ttacgttgcg ccgcattgat
    20461 ttcattcatg gattgcttaa cttctgcatc tggttcgacc ttggtaatca aggttttcac
    20521 gataatgtag ccgtaagtgg tcatttcttc tgctacttgg tgttgaacct caagggcaat
    20581 ctcatctttt ttctcaaaca attcatccaa ggttaatttt ggaacagaag agcgaagagc
    20641 atcttcgata taagatttaa tctgagattc tggacgtatg agtttatagt aagcatctgt
    20701 cacgctctgc tcgttgacac ggtactgagt cgctacattc atcataacga acacattgtc
    20761 cttggtctta gtctcaacca caatatcact ttgcaacaag cgcaactgaa tccgtgctgc
    20821 aatcgagtca atcccaaaag gcaagcgaat atgaataccg ctattagcaa ccttttggta
    20881 tttcccaaag cgttcaataa tcgccaccga ctgctgacga accacataaa ctgtactcag
    20941 tgtgactatc accaatagga gcacacaaac aatcagaaaa atcatgaaaa atattgccat
    21001 aatggaacct ccacaagtat ttttctagta ttatagcaca tttaaagaag gctgtgccgt
    21061 ttttactgcg atttttcctg aaatgtcaat aattagaggt gaattgtcct attgtcgtcc
    21121 aatctcttgc taaaataact ctttataaaa ggcaatcgtt tcttctaagg ttggcataaa
    21181 tggatttcct ggtgcgcagg catcaatcaa ggcattctta gaaaggtatt caaagtcgaa
    21241 atctttttct tcaataccaa gttcagtcag tttcttagga atacctactg tctcagaaag
    21301 cttctcaatc tcaacaatcg cataatcggc acattcttga tctgatttac cttctacatg
    21361 aagtcccaag gctttggcaa cattgcggaa agcttctggt acacgtttag cattttcacg
    21421 ttctataact ggtagcaaca tggcacagca aacaccatgt ggcaaattat atactgcacc
    21481 gagttggtga gccattgagt gaacataacc aagaccagca ttattaaagc tcataccacc
    21541 aaggaagatg gcattgacca taccttcacg cgcttcaata tcatgtccat tttctacagc
    21601 acgaggaaga tattccttga taagctcaat agcaccaata gacagtttct tggttacatc
    21661 ataagcacca ggtgttacca aagcttcaac agcgtgagtc agagcatcca taccagtagc
    21721 agcagtcagg cctttaggtt tggaaagcat gagttccggg tcattaacag agataagagc
    21781 aaggctattc ttgtcaacca ttaccatctt aaccttgcgt tcttcatcag taatcacata
    21841 gttaatggta atttctgctg atgtacctgc cgttgtattg atagccacaa ctggcaagcc
    21901 ttttttagca gacttatgaa gcccttcata atcctgtggt tttccaccat ttgtagccat
    21961 gatagaaata caactagctg catcctgtgg agaaccccca ccaagactga tgataaaatc
    22021 acagccatgt tcttgcaaag cagctacccc atctgtgaca ttcttacaag tagggtttgg
    22081 ctctacatcg ctaaagatga catattcgat tccttctgta tccagtggtt ttaaagtctt
    22141 aggtaaaata tcactgcctt cgatgtactt atctgtaacc aaaagtgcct ttttatagcc
    22201 aagttccttg atataaggac ccacttcatt tacaacacct tttccaataa ggttgactgc
    22261 cggaacataa aatgtagcca tataattttc ctcctgcgcc tccgcgctat ctatttaaca
    22321 tcattatatt actttttgta ttcgttttta attatttgtg aattgtttaa caaatctttt
    22381 taaaaggata ggagggacgt aagttcacct cctatataaa caagttttat ttatgtagtg
    22441 gtcccaacaa ctgacatgca cgatagtctg ctgattctag atcttctgtt ccaaataagg
    22501 accaattttt aggtctaaag atatcttcct caggtacatt atgcatattg acaggaattc
    22561 tcaacataga agccaaggta atcaagtctg ctccaatgtg tccatatgtt atggctccgt
    22621 gattagctcc ccaattattc atgacgtcat agacagactt gaaagcacct tttcctgtca
    22681 aacgtggagc aaaccaagta gttggccatc ctggatctgt acgattatct aaagtatggt
    22741 gaacatcttc aggaagttca agtgtataac cttctgcaat ttgtagcact ggaccaaccc
    22801 cttttagaag attgagacgt accattgtta ctggcatatc ccccttcgtc aagaaacgag
    22861 ttgagaatcc tcctccacgg aagtattcgc ggtttgctgg tgggaagtct gtattttcaa
    22921 gcatagcctg cacttcactt tcatccaact cccagaatgg tttcataaca ggtttgccat
    22981 ctcgagtagc ttgccctgta ccatccaatg tacaagaacc agagttgatt agatgtaaga
    23041 agccagctgc agcacgaccc tctaaagtat gtcccgttac acgtttaaca gcctctggac
    23101 tccaataagt gcgcacatca gcaaagattt gtggagtatt tgttaataga taattaaaga
    23161 gcatagacac accatttagt gaatcattct ctgtcgcaaa tacaaatggt tttcgaatac
    23221 cattccagtc aaactgagta ttgaggaaag tttccataaa gtccccattt gggaaatggt
    23281 ctgtccactg gcgttgacct tggaaaccag ctactaaagc atggtgacca actgcttctt
    23341 cctcaaaacc aagttcagca agtcttgggt taccaaccat taagtcacgt ccaatcatga
    23401 acatcttaat aacaaattcc cattgttttt ctttctcttc tttagaaaga atcaaatcct
    23461 cacgatttcg gtcaactcct tctttgatat gttcttttac ccaaaccata gcacgttcaa
    23521 actcttctgg gtcgtaaata ccacggtcca tacggcgcgt gaactccgtc atatctaccg
    23581 attcatttcg cattcctaag tattcttgga agaaatctgg atttacaata gaaccgccaa
    23641 tccccatcga aacactaccc attgataggt aagcagtgtc tctcatcaag ccagttgcaa
    23701 gagctgcacg cgcataacgt aaaagttttt ctttgacatc ttctggaata gctgtatcat
    23761 tagcttcctg aacatctcta ccataaatcc caaaggctgg aatccctttt tgagcatgtg
    23821 aagctagtac agctgcaaga tagacagctc ctggacgttc tgtcccatta aatccccaaa
    23881 tagcatgagg aatatctgga gacatatcca tagtttcact accataacac cagcatggtg
    23941 taactgtaat tgttgcgcaa acatttgatt ttttaaacaa ctcatgggaa gctgcagcct
    24001 ctggaacacg accaatagta gatggagaaa tcacgcattc cacaggttcc ccatctggat
    24061 atttcaatgt gcttgaaatc aaatctgcca cacttttagc catgttcatt gtttgcactt
    24121 caagtgattc gcgtacacct tgacgacgac catcaatagt cggacgaata ccaatacgtg
    24181 gatgttgaat catactattt tcctcttatt tttctgtttc tttcatacta ccatcgccat
    24241 tatatttacg gtagcctggg tgacgacctg taaccttata attttctcgc attgagaata
    24301 gacgttctaa agtcggacga gcaatttctt gctcctcaat gccctttgta gaaagtaaca
    24361 ttcttccgtg gaaggttgtc tttgcgacta attctaaagt ttccatacgg tagtatgctg
    24421 taattacatc gcttccgaca gtcagagctc catgattttc taatagcatg acatcatgat
    24481 cgggcagata aggtgtaatt gcttctggca cttccattgt agacggaact ccaaatggcg
    24541 ttataggaat tgcccctacc acaatcgcgc tctcaattag tgaataagta tctaaaggaa
    24601 tgtgtgcaag agcaaatcct gttgcaatcg gtggatgcgc gtgaacaact gaacggacat
    24661 cctcacgttc ttcgtagcac caaatgtgca ttttaatttc actagaagga cagtaatcac
    24721 cttctgcttc taaaatctct cctttaagat ttaacttcac cagcttttct ggtgtaataa
    24781 aacttttgct gataccagta ggtgttgcaa gaatggtatc ctcatctaat cgaacagata
    24841 cattcccatc gttagcagca acccaaccaa gttgccacat cttatgacaa acatcacaaa
    24901 tctgttcacg aattaatttt tcatcttgag acataaactc actcctttct aaaaatgaag
    24961 ttcgaattca agatttccgt tactgttaat ggtaatagta accatatgat ttttctgatc
    25021 aaaattgaga gatggctgat cataggaatt atttgttcct gataatgaat gaatagtagg
    25081 aagtttatct atacctctta atactatctt tgtaaccctt ttctctccaa actgagtatc
    25141 tttgatataa tgttcttcaa tccacttaat cgcacctttc ttggtcaatt gtggtagttt
    25201 ctttgcctga tttgcatctt gagcattaga ccatagtgaa tctttatttg ttctaaaatt
    25261 atttaaattc actttcaatc cattacttat tctctcaaaa ataccatagg tatgaggttc
    25321 aaattcaaca tccatttcta aagaattaaa ttttaatttc ccagtctgtt taacattctc
    25381 atttaccttg taattataga caaaccatct attatctaat ttctgagcaa agatatctcc
    25441 ttcataattc ataggataca gcttatttaa gtattctttt ctagcctgag tagagctaaa
    25501 ttctggagaa gtgatttcct taatctgaat acggctacca gaaacagact ctttcaattt
    25561 atctgttttt aaaacacctg gaatagctgg aataacatta taacgaccag tcatgtataa
    25621 tggagtttgt tctctatcag tatttacgtt tacaaagaac ttaccatttc ctttatttga
    25681 atagtctcca tgtatgaata cttttgtatc ctcaagtacc ttttctttgg atggtgctgg
    25741 atgcgctatt acatatctaa aaaattcttt tattacttcg ctaaagagta agctttcttt
    25801 attatttaca ccataggtat aagctgggtg ttcaaaatta taaactacac ctccattaag
    25861 gtaaacccct aaagcttctt ctcctagcat agactctggt tcagtcaacc attgtctatc
    25921 tccttgactt tttccgatat taccacttgc aaacagtttc catttacctg tctcatacca
    25981 cttccaagta tccatcaaac caccccattg ataagtatga tttgaaagcc aaagtccctt
    26041 catataactt tctgtagttg aatcatttcc ctcagcagca ggtgtatttt tgaacatgaa
    26101 aataaggttc ttccagtact tatcaacaga tttctgaaat gctatcttac cgttttttcc
    26161 aaatgctttt tctattgcag aaccattatt ttgttcagcc catataaaat atcctccatt
    26221 tttagctgat acttttaagt aatctgccgc cttattttca atatcatttg cccaaatcca
    26281 ataattctct gtactgaaaa taccatgcaa atttggatat ttttgataca ttttatcaat
    26341 ccagctggta cttaaccaat gtgccgaagt ataataaggc atattaccag cagtgtaaac
    26401 cgtcagaata actggaattg gttcattttt tccagtctta ggatttacat atttcgctgc
    26461 ttcttctaac atatgttcat aaaaatctct agcagctcca tctcttttag taagatcatc
    26521 tggatgtaac tgtataacag tatatggttt taagtcatct ggtatattct cccacttacc
    26581 tgttaatgta tctcctcccc aaaaagtata tttcccttgg ttaaactctt ctccgttagc
    26641 atataaaggc atcataagta acggatgttc attattaatt tcacgacgtt tatcaacttc
    26701 tgaccaatca ttaggattct taaatttacc atttgccata aacaaggctc cacctaaaac
    26761 caattcgtca gcccatgtat gtttccctga atatgattta agttctattg tctgtgtatc
    26821 tttgggaatt tcaacatcaa ccagaaatgc cgatgtatca tacttaattc cttttggata
    26881 ttttgaatct gatgagtaaa tgactttacc atccgcaaca acttcaaatt tttgaatatc
    26941 tgcgtaatct gaatttaaat gattggcgct cctatcaatt ccgacatagg aaataaatct
    27001 tgtaacatta gcacccgaaa catcatattt tatactagac gggttgcttg ctacagttcc
    27061 caatccctta ttaaattcga cttccttacc gtcaattagt aattttattt tattattaga
    27121 cccgttatta ccaggtgtaa acggtttatc tttctgaact gtttttgatt tatcgtcatc
    27181 accatgagta gcatcaaccc attctaaatc agaaagatat actcctccat taattccttt
    27241 attaagaaga tctggagtta tagtttgagt actaacagtt ttaataagtt tagcatcagc
    27301 aaatacaacc tcatcacccc atgtatgttc tcctgcgaat gattttaagg atattttttt
    27361 agcgttttgt ggaattgtca cactaataaa ttgtgcctgg gtattatatc tgaatccttc
    27421 tggattagtt acactactag aatagacaac ctttccatca atttctattt ctattctatc
    27481 cactactgca tggtctgatc tagaactatt agcactttga tcaattccta tataagtttc
    27541 aaatttttca aacccttgac cgctgatatc atagctgatt actgatggag aatctgcgac
    27601 tgttcctatt cctttatcaa aatatttaac tttatcatca gaagtgagca atgaaatctt
    27661 agtatgctct cctttatttc ctgttgtaaa tggagcatct ttttgtactg tttttgtttt
    27721 atctatatca ccatgagttg cagaagacca atccatatct gaaagatatg tcatctcttt
    27781 atcaatttct ttatctttag taataagttt ttctaagtta ttccctgtta ctctagcagt
    27841 ttcagtacta gctctgttat tagaattact ttgaactaca tctgcataaa ccaaattact
    27901 tcctaaaaaa cttgttaaaa cagccgtagc tattaaaatc tgttttgcta tttttttact
    27961 ttccattttt atttcctcaa tatttacata caacttcttc aataacaaat aattttgtaa
    28021 gatattctgg aacacaaagt gctaaattac ttaaactgac ttttattcca gcattcatta
    28081 attcatctcc ataaaagaac tcatcaaatc tagagacgga atagcattta tctttatcta
    28141 aaccactaaa ataaactctt gtataacctg gattcaactt actattcaat tgagcataca
    28201 ataaaatcga ttggcattta tcttttgaaa caacttgcca actagcagaa tcacaactaa
    28261 aaggactgtt tatcctatag aaatccccat attgaaacaa tgaacgatat tttttataaa
    28321 actgaatttg ttctctcact tgttcttttt cttctaccga caatctcgta acatctaatt
    28381 cataaccgaa agaactaaaa taagctacat ttgtcctagt gtccaaactc gtttgtcttc
    28441 cactttgttc atttggagaa atagaaacat gggctgtcat cattgatgga gaatatccat
    28501 aagaagttcc atgctgaatt ttcaatcttt ctatcgggtc cgtatcatca ctcgtccacg
    28561 cttgcggtgc ataatacata agtcctaaat caaaacgtcc accacctgaa gaacaagatt
    28621 caaatagaac tgaagggaac ttagttatta aacgatctaa taactgataa agacctaaga
    28681 tatatcgatg accaaattcc atctgctgtt cagaatcaag ttcaatacta taaatatctg
    28741 ttatatgacg attcatatcc catttgatat aatccagatt tgttttgatt atgattttag
    28801 aaatagaatc aaaaaggtaa tcaacaactt cctgtctcgt caaatccaac acatattgat
    28861 ttcttcctac tgacttctct ctgtctaaca aatggatagt ccaatcggca tgattcttgt
    28921 acaaatcact atcaatagaa atcatttcag gctcaaacca caaaccaaat tgtaaaccta
    28981 ttttgtgaat ttcatctgca agaaatccaa taccttcagg aaggcgactt ctatctgtta
    29041 cccaatcacc cagagaactt ttgtcatctt tcctatgacc aaaccaacca tcatccagta
    29101 caaataattc tatccctaaa tctttagcag cctttgctaa atctaaaatt ttttctgtac
    29161 tcaagtcaaa ataaaatgtt tcccaactat tgagtattat aggacgacta gcttgagaaa
    29221 aattaggatt aactaaatga tgtcgataaa aatctgatag ttgttggctc atctgattca
    29281 tcccttgatc agaatagatc acaattgctt ctggtgtcac aaaactttcg gcaggattta
    29341 ataaaaaacg aaattctact ggatttattc ctaccgttac tctagcggtg tcaaaatggt
    29401 caacttcaat catattttga aaatttccag aatagataaa gttaaaacca taaacctcac
    29461 cactatgctc tgtagtttcc ctccttgata gcataaagaa aggattttgc tgaggacttg
    29521 atgagtatct caagctatct attgcttgaa tacctggtct aagcgaagtt ctatataact
    29581 gacgttccct cagccatgct ccagaaaatt gaataaagtc atattgacta tcaggtagat
    29641 ctagctgtaa gctcattgct ttcaaaagag tcaacttatt attgctatta tttaaaaatt
    29701 ccgtatttct aactaagaca ttgtattccg gaaaaattgt gtaatttagt ctaacagtta
    29761 aatctaatat ggaatctgtt aaaataatcg ttaatgtttc cacacactga ccaatatcat
    29821 caaatgttga aggtagagag ttaagacgat ttttcccttt ttcaagttca aaaccttgat
    29881 attttagtgt cgttatccta ctaccattca ttccttgaac ttgaaaggct ggatgccgaa
    29941 aatctccttt cccatatgaa gaatattcat tttgtatcat agactgttga atatcctcaa
    30001 attcttcaga aaaagatact tcaaatcctc ttttttcata agccatcaat tggttataat
    30061 tttccttgac atgaatcctt ttcccaaaat gtagatgttg aagttcccca ttttctaaaa
    30121 ttctaaaaat ataactacta tctttcattg ccaaatgaaa aacggtctcg tccttattaa
    30181 tataaatcgt catctctacc tcacaatttt ttctttactt cttcagtatc acgatagagt
    30241 acggtgccaa ttctatctca tcttcttgaa tagtaaaatc tccaattact ctggtaaaat
    30301 tctctatcga aagattctct atttttattt tttgtttaat ctgagcagga tttctcagag
    30361 agagaatttc agtagaagtc ttctgattgt gacacttata accataaatt tcaaattttt
    30421 caggacttcc tcctattttt ttactatatt ttagtgtttg ataattctcc tctatccatt
    30481 tgacggcacg agcattggct ttccagcgtt cttcatcaaa catatcaaaa gaatagtgaa
    30541 actcccaaaa agcattccct cttgttgaaa taaataatag ataagcttca aaatctggta
    30601 ttgatgcaaa catttgatga tccatgtacc aggtatttgc actgacagca tagattggtt
    30661 catgattata aatcgaccac ataggtaact gtatctcacg tttttccaaa aattcttgat
    30721 actgaatatc tcggtaagta atcatacgat tgatatcatt acctgcattc tctgtaaagc
    30781 ctacatcttg agatatttga atccataaac tattgaccca ctgtaaaaac catggactag
    30841 gatttacata agaagtcaag tttaaccaac aatcttttcc tcctctctcc tttcttagat
    30901 ctatcaacag ttgaattaag aactcataaa ccgctgtcat ggtatacata ccgtgcggtc
    30961 cactcttatc aggtttgtca ggttgaagta accaaccatc aattttccaa tagctgatat
    31021 cgaattcttt ttgatattcc aacatctttt tcttcatttg attgagatag ttaaaatcag
    31081 ccacgtttac atcatttgaa atcaaatttt tagatccaat atttaactct ggatgtgctt
    31141 ctaaccaatc actcataatg atttctgtcc cattgtaacc acctcgggga ccaatccaca
    31201 atcctaggct tgaaccaaat ccatttacaa gatatttaat atttctcaaa ccatttggga
    31261 atttatgatt gaattcccaa actgattgat agtttgtcca accatcatca acaacataag
    31321 catctaaatg aactccatga ttttcaaatc catctcgaat ctcagaaaaa ctttttaaaa
    31381 taccttcctc tgtaatatcg gtcatatggt cataccagga attatactgt ttacggaaat
    31441 aactaggttg agctattcct tcaacatatt caaaaaatgc ctcttgaatc tcttcttttt
    31501 tataagaaca tgctccccca ataatacaag accacaaact cttttcttga tttacaacag
    31561 ttcccaagta atatcttgat acatagtgtc taccatctac cttgttttca gacattggaa
    31621 attccattcc taagaataaa gaattcgcat aaacaggttg tcccaattct acataataac
    31681 cggaaaaatt cgccatttcc ttaatacaat cttgtctttt cggatagaaa atattgtctt
    31741 ctgtttcaaa ctcaaaactc tctacctcta cagatttaat aacctcagta gatgattcta
    31801 aaactaattg cttcgaaaga acatcatccc tcgattcaaa gattaaacgt aaacgaatgg
    31861 aaggacaaac aaaagaaacc tgaattcgcg tatctccctc ttccctaacg attgctgaaa
    31921 aatgatttga agaaagtctt ataccattct ccaatgtaat ggtaaattct tctttcgaaa
    31981 catttttatc attactcctg cccgtttgtt tacgatatca gtagtataaa atcgaccatc
    32041 tccccaaaag aaattacgtc ttacattttt attttcaatt ttcatataaa ctactctctc
    32101 aactcaattt tgattacgct atcaatcaag tctggtaatg gataggtaaa atgtggaact
    32161 tctccaaact gtgcaaaaca aattcctttg taggcattgg tcgtccagct ttctgaaatt
    32221 ttcacctcac ttccatcatg aagaaagctc attcttttta cgttttcttt actaatacca
    32281 agaagagcta aaggacctat aggttgttca aatacatgag cataaaccgt atttccatta
    32341 cgagtatagt atccccaatc tggttttggt aaattttcat ctccactaca tccataaatt
    32401 gcctcaccat ttcgagacat ccatcgatga aaattatcta atattttctt acttgaatca
    32461 ttaattcttc ccaaagcatc tgggcctaca ttaagaatca tatttccgtt tttactaaca
    32521 cattccacta actttcttat caaagtttga ctagacttat atagataatc tgtaggatta
    32581 taagcccaat tgttattcat agttagacaa agttcccaag gaacaggttc acctttaaag
    32641 tttcgaatac cttcgtgagg aacaatttgc tctggactaa caaaatctcc agcgtaagag
    32701 gtaatttcat ctgtaacgat acttccgaat ccttcacctg atgtttctaa tctattatct
    32761 acgataacgt ttggctgata atgacgaacc atgtctatta attttgaagc tccccatttt
    32821 tcacccacca tgtcttcata agaataatca aaccataata tgtcaatctg accataacca
    32881 gttacaattt ctttaacttg attgtgcaaa tactccaaat aacggtcaaa gttaattttc
    32941 tcatctcgat aggcttcatt tcctctcatt ggatgattta gatcagcata tttagggaaa
    33001 tcaggatggt gccagtcaat caaactaaaa tataagccta cttttaaacc ttcagctcga
    33061 acagcatcta caaattcttt aactaaatct ctacctgcag gtgtatttgt tgctttataa
    33121 tctgtaaatt ttgaatcaaa caaacaaaac ccatcatgat gtttagccgt taaaaccata
    33181 tacttcatcc ctgcagcttt tgcttgttta gcccattctc tagggttata ttccttagga
    33241 tcaaaagctc gaaaatatgg ttcataatct tcaatagata atttctgatg actacgaatc
    33301 cattcccctt ttcctggaat agaatataac ccccaatgaa tgaacatccc aaatcttgct
    33361 tctctaaacc actgagttct actattgata gtttcaagtg tatgtttcat ccttttactg
    33421 ctcctcctcc taaagcttca atgaagtatt tacttgtaaa tgcgaataga atgattggtg
    33481 gtaatacgat tacaacaata gctgaaaata gaccattata attagtatta aaacttgtag
    33541 taaattgacc tagtaatgct ggtactgtta attcattttt atcaatcacc aacatagatg
    33601 cccaataata ttcattccag ttattaataa aggcaagtac tgctgctgtt aaaattcctg
    33661 gtctagcaat tggcaacatc actttccaaa aagtttgagc atatgtcgca ccatcaatct
    33721 ctgctgcctc ctccaattct tttggaataa tttcaaaata gttctttata ataaagaaac
    33781 tcatagcaat tccactacaa gagtagacca gaattagagc aactggggta ttataaatcc
    33841 ccaactcatt tactaatcta taaactggaa aagtcatagc tgttgctgga ataaatagag
    33901 tagaatacag catagaagtc accaacttct tccctctaaa ttccatccta gcaataacat
    33961 atgaagacat ggagatcata acaatgctaa taataactga tatagacact actttaaagc
    34021 tattccaaaa gtaagtaaaa acatgaagtt ttgtaaaaac atttatataa ccttcaagag
    34081 taaagtcaat cggtaaacta atacctggat ttttcatagg atctgctttt aatgatgata
    34141 aaaaaatcca cgcaataggg aaaatagaaa ttaaaatggt aaggagcata taaccatata
    34201 taagaacttt gcctttttta ctaataccat ttatatgtgt attcataatc atttcccacc
    34261 tttcagtctt tttctttctc tgaataaaat attaatacaa acgataatca acattccacc
    34321 taagatttgt ataactccaa ttgtatttgc acgagcatat tgagttcgtg agctagccac
    34381 tgctaattct cgaataacaa aactgatact ttttgttcct gcagcaccat ttgtcaaaaa
    34441 gaatacttcg ttatagagca aaaatcctga agttgctgcc ataattgaaa cagttttaat
    34501 tgtcccctta atcatcggaa tcgtaatata ccactctttc ctccaaccag taatattgtc
    34561 taaaatagca gcttcttgaa cttcttctgg aatagcaaaa atttgcccaa gaatcataat
    34621 ggttgatgct cctgcaaaga agatataagc gccagtcata gctactatat ttaatcctgg
    34681 agtcaacagt actgaatctt taaacgatgg attaaataga tgaataattt ggttaacaac
    34741 accatagttt ggattataga tttgaagaaa aatcaagccc atagcagcac tagaaatgat
    34801 acttggtaca atataaagat tcctagatat cttccagcct tttaatttct tagataatgt
    34861 aattgcaaca gatacagcta aaggaacttg aataataaca ccaattacag cccaacgaac
    34921 agaattaatt agagcttctg taaaaaatgg gtattcttta aaaacatacc tataattttc
    34981 aaataattta tcaaatccaa gaaactcagg ggtgtttaaa tttgtataat cccatttagc
    35041 aaatgaagtt aaaaagattg ttactacaga ataaaaataa aatagaaaga aacaaatcaa
    35101 cgttggcaaa agaaataaaa agataaatcg tcttttttga ttcttcctat tttgtaggct
    35161 cataattttt tgtgccataa acaaactcct tttttcaact atataagtaa aatcaaggtc
    35221 ttatcaaaag aaagcacgga tacgtgaccc tgtcctctat gaacacaatt gaactttctc
    35281 atcaaatacg ggaaggagaa agtactaggc aaaaaagact aatcgctagt ctcgctaaag
    35341 actttattcg gtctggtatg tgtatctatc tggattccag tactactgtc tacgaactct
    35401 gtccctatct ttctgaactt gataatttga ttatttttac aaatggttta catactgcac
    35461 aaaccctatc tgaaactgtt aaagatagct ccaaaatctt tatcacatct ggcgaggtca
    35521 aacatcaatc ctgttccgtg gtcaactatg ataaggaaaa ttctttatta gatcatttta
    35581 atatcgattt agcattttgt tcagcaagag gtattgatga ccaatatgtt tatgaagctt
    35641 ctctcagcca agctatttca aaaaagaata ttattgacaa agcccatgaa accatcttac
    35701 tgattgatag ttctaaattt tacaagactg gattttttaa aattaatccc ctctccaaat
    35761 acacaacctt tatttctgac accgtgccag accaaaaatt attagacgca gtcgaattat
    35821 ttgatggaga atgggtttct gatattcaat gaaaatcaaa taggaagcta gtctcagctt
    35881 gctcacagta cagatttgaa aactgatcaa gtcaagtccc ccatggacac ggcaagacga
    35941 agctgacatg atgtgaagag attttcgaag agtattcgtt ctgtctgctg actgatttac
    36001 aaatattcac cacatgacat tattttctta gcaattctaa aaacagaggc tggacaaaaa
    36061 ctcaatttca ggagaaaatg gagtaaacct tcccacaata aaacgcatag tatcaagttt
    36121 ttgcacacct gatattatgc gtttttctga ttttaaagac tttgtcgcct agaccctata
    36181 tttcaagaat taagtgacct tgatgatcta aatatattgt ggtatactat ttttgtcatc
    36241 ggttaccgat tacgttccta tgggtttgtg agaggaggtg aaactaatga gccttatcat
    36301 agagctcgct cttactatta tagcggatgt tatagctgga attatcttgt atttcgtctg
    36361 cagatggcta gatagtaagg agtagaccga ctgactagcc tataaacacc cgttaaatcg
    36421 ctaagatacg tcaaaaaagc ccttaactac ggccatagtt aggggctttg gtgttctaat
    36481 gaaccttatc aattaactac attttagcat ataatctaca atagttcaag cattaagtga
    36541 ggttgatgat ctaaatttag tgtgatataa tacttttgtc atcggttacc gattacgttc
    36601 ctatgggttc gtgagaggag gtgaaactaa tgagccttat accagagctt gctcttacta
    36661 ttatagcaga tgttatagct ggaattatct tgtatttcgt ctgcaaatgg ctagatggta
    36721 agaagtagac cgaatgacta gcctataaac acccgttaaa tcgctaagat acgtcaaaaa
    36781 agcccttaac tatggcacta gttaggggct ttggtgttct aacgaacctt atacactaac
    36841 tacattctag catataagcc cagatatttc aagagtttta tttatttttt caggttccct
    36901 tagttctgaa aggtctataa tgaagttagc catctagtat caaaaaaccg actagctctt
    36961 atgaactagt cgatttctca tcaatgcgcc aacatttctt gggcgatttc ttggccagat
    37021 aggttatctg ggtagtaggt tggccagttg tccatttctt caaagagggc ttcttggctt
    37081 gtgcctccaa agaagatatg gaaatgttct gccttaactg gggcaacatt gtggtcacta
    37141 aactgaacat acttgaattg tccagcgtca gcatctgtgg cttcaaagag gaaacgcacg
    37201 ccacgattgc ctttcttgta agtcaaaatt ttcttaccga catacttgta agtgtatttc
    37261 ttgctttgtc caccttgaac aaattccata gtattatcag taatgttaat cttagtcaca
    37321 tctgtctgat agccttttgt atagtaagcc ttgtactcag cctgggtcat cttaccagtt
    37381 aacttagcct tgtagtcaaa gacttggtca aacgtgccgt cttcaaggaa aggataaact
    37441 gattgccagt tacctgcata gtcactcaag gtacggtcct tgacagctgc atcctcgaag
    37501 taaccatttt ggactgtctt ggtatcctct gccttttcag gttcgattgc tgggccttct
    37561 tggtctgttg tttgtttcaa agccttgagg tttttctcca tcacggaaat gtagttttct
    37621 ccagccttgg tgtcctcttc tgtcagactt tctaaaggat tgaggacatc agttttgaca
    37681 cctgcttctt ttgaaagtgt gttagcaagg gcttgtgagg cattttcttc aaaatagata
    37741 taggcgattt tatttttctt gacatactct gtcaattctg ccaagcgagc agctgatggc
    37801 tctgcatctg gagaaagtcc tgagattgcg acttgtttga gtccatagtc caaggcaaga
    37861 tagttaaagg ctgcgtgttg agtcacaaag ctcttttgtt ttgcttgaga caagccttct
    37921 gcgtaagcct tatccaaggc ttgcaatttt tcgatatagg cagctgcatt cttctcaaag
    37981 gtctcttttt tatcaggata atctgctgac aagctgtcgc ggatgtgctc tactagttta
    38041 atggcacgaa ctggtgataa ccaaacatgg gggtcaaact catggtgatg accttcttct
    38101 ccatggtcat gatctccctc ttcttcctcg ccacctggca agagcaacat atcgcctgtc
    38161 gccttgatgg ttttcacttt tttcttatcc aaggtatcta gcaatttagg tacccatgtt
    38221 tccatgtttt cattttcata aacaaaggta tctgcatctt ggattttggc aactgccttg
    38281 gcagatggtt cgtattcatg aggttctgtc ccagcaccga ttaggagttc tacattagcc
    38341 gtatctcctg cgacttgctt ggtaaattca tagacagggt aaaaggttgt cacgatattg
    38401 agtttaccat ctgcctgttt ttgattggaa caagccacta aaaacaaggc acatagactg
    38461 gctagtaata agctaatttt tttcacgttc gtctcctatt tgataaaacg tcttactaaa
    38521 ctgattagta taaagacagt tacaaaaata atggtaatac ttgcacttgc aggtgtttct
    38581 gcatagtagg aaatgtaaag tcctgctacc attcccaaaa agccaatcgc actggcaagc
    38641 agcataaccg atttaaagtt tttccccaga cgcagggcaa tactagctgg caagaccata
    38701 atggtcgata ccagaagagc tcctgctgca ggaatcataa gggcaatagc cacccctgtc
    38761 accatgttaa aaagaatgga catggtacga actggcaagc catccacaaa ggccgtatct
    38821 tcgtcaaaag ttaagatata cataggacga agaaagagaa aggtcaaaat caaaacaacc
    38881 gccgcaatga caaagaggga aatgacctgt tcttcactga tagtcacgat cgaaccaaag
    38941 agatattggt ccaaactcat tgaactcgag cttttaccct tgctcatgac aatcagagaa
    39001 acagccagac ctgttgacat gaggatagct gtcccgattt ccataaagct cttgtaaacc
    39061 gtacggagat actccagaaa gaccgccgca atcaagacaa tggcaatagt agaaacagtt
    39121 ggagaaatcc ccaaaaccag accaaaggct acacctgaaa gtgagacgtg gctaagggta
    39181 tcactcatca aactctgacg acgcaagatg aggaaggttc ccaataccgg tgagaaaaga
    39241 ctcatagcaa taaccgccaa aaaggcgcgc tgtataaagt cgtaagataa taaactaagc
    39301 atggcccacc tcctggccat tctcatgaac gttgaaacaa cgccatggcg agtcttggtt
    39361 acggactaga tgaatattgc gatccgcata atccttaact tcttcagggt catgggtaat
    39421 catcaaaaca gccttgccat gatgatgggc gctgtggtgc atgagttcgt aaaattcatt
    39481 tttacttcct gcatccatcc ccgttgttgg ctcgtctagg ataaacacat cagggtcaga
    39541 agcaaacata cgcgcaatca ccgctcgctg cttttgtccc ccagatagag accccaagcg
    39601 tttgtctcga tgttcccaca tgccaactga gtccagactg gccttgatat gctcctcatc
    39661 atgagcattc aaacgacgga accagccttt tctcggatag cgacccgact tgacaaattc
    39721 atagaccgta cttggaaaac caacattaaa actggcaatt tgttgaggaa gataggctat
    39781 tctcaatttc ttaccttgcg tatttgtctt tgaaatagcc acctttccaa tgcgtggttg
    39841 cagaattcca agactagcct tgatgagcgt cgtcttagcc gctccatttt ccccagtcaa
    39901 ggtaacaaat tccccactat caacactata attgatatgt tcaagaacag gctccttatc
    39961 ataatagaag gacaaatcct ctaccgtaat atatctcatt atttgatttc tcctactaaa
    40021 gcagtcaaaa accgctgaat cactttttgt tcatttggag taaactgagt cgccacttgt
    40081 tcataggtta aaagtgtatg ctcatggtga tggtggtgct cctcagcgat tggacgagcc
    40141 aagtcagtca actgataaaa aatcacacgc gcatctttag aatctttaga tgtttccaac
    40201 atcccttcct tgaccaaaga cttaatggcc ttggtaactg ccgcctgact gacattgaga
    40261 cgacgggcca attctgaatt tgttaaagat tcctctgaca agagcataag gatatgctcc
    40321 tgagtattgg tcagggccac ctcgctagtg caatgaccta ttaggatttc atgctgattt
    40381 tccgcctgca aaatcacctc attcaaaaaa gcattgatat cctttgctag ctgtctcata
    40441 tctgactcct ttccttttag acttctcttt tttaagagaa aaatactatt ctttgacatt
    40501 ttgtttacca gttaattata tcacaagcaa aaaaagagtc aagaaaaaac gtgaaaacta
    40561 gtttcattct tgaactcttc tatattatat tatctattga aattctttga catctccatc
    40621 ataagtcgcc caatctttgc tgaaaaagcg ctcattcaga tggtaagtcg gagctggtgt
    40681 gggattggat aggaaaggat caactgcctt gtcaaaagcc aaccaaccca accagccaag
    40741 gtgaatggtg tccttcataa agaaaggctc cccgccgtcc ttagaaaaat ctgctatatt
    40801 ggtaaaacct tgactttcta actggtagcg aatcttctgc accgtttgtt gatacatatc
    40861 ctctcgtaga ccagcatagt ccatccattt tttattaaca ggtggaatga taaaaatcgg
    40921 gtttacctta gatttagaaa actgtgttaa aaccaactgc aagtcattat actctggcga
    40981 cttgagatag gtaaagcttt tctgagaatc ctttaatttc ttcaaatcct tcttgatctg
    41041 ctcattatag aaataatttt ccattcccat ctcattattg gaagtatttt tttcagcatc
    41101 tgctttgaca acatcttcta ttgcttgata agaaaactgg tctggcaaga tttttaaata
    41161 cttagctaca tgcttatcgt agttaacata gcctctaacc gaaaactgac caaaaaagga
    41221 ggcttggcgt tcattaaaac gagccaataa ttcaatcatt tcattgtctg ctgtcgacaa
    41281 ttcttcttta cttgccaact tctgaaccag gtccttcata gctacgtttg ggaactgttg
    41341 cagtaagcga gtcgctgcat attgactagc ctgatcccca gattgatgtt tcagaaaact
    41401 agtcaactgg tctccattaa aatactgctg gaaggctgct ggatcatagc catttttact
    41461 gaaccactga ggtgagataa catacacaac ttgtttattc tccagctgtg gtaacatctg
    41521 ttgcattcca aaatattggt taagcgatgc agctcccccc tgtcctaaaa gataaggacg
    41581 gtaggaacga ttgtatttct cagctaatac cgcaggatga gcaccgtcaa aacgaagcca
    41641 ttcactagag ccaaagaagg gaacaaaacg cacatttgga tcagatagtg ctctgacttt
    41701 ttgacttcgc tccttaaaac tatcgatagt agtagccact gctgaacgct tttcagctcc
    41761 tagattatga tgcatctcag taggataaaa gaaaatgagc agaaaaacca acaaaccagc
    41821 gatcaagacc ggtccgaaga tcatccataa gcgtttaagc attttgtagc tccacaatac
    41881 cagctatgat tttattagct gtattccagt cgtcacgacc aaactctgtt acagggacac
    41941 gaatgtcaaa acggttctca atctccacaa tcaactcaac cgttcccata ctatccaaga
    42001 cacctgcatc aaaaagatct tcatccatca tgtcagaaac atcttccata aacaactcat
    42061 caataatttc aataacttct gatttgatat ccatatttta tttcctttta ttttttaaac
    42121 catagattat tcaagaatcc agaaaagatt aagaatgaca acatgacaac atggaaagtg
    42181 ataaccatgc caagcaactg aatccagcga ttctcaggta gggcagcctt ccctgctttt
    42241 ttccgttcct tattgagcgt ttttttcttg cgaacccagg catcattgat gaccaagcct
    42301 agtccatgaa agagtccata ggcgatatag taccaggtca caccatgcca aaatcccata
    42361 atcagcatat ttacaatgta ggccatgctt gaggttacat tacgattttt aaagactttc
    42421 tttctggtta acaccatcac cattcgcata aagacaaagt cacggaacca gaaggacaga
    42481 ctcatatgcc agcgattcca aaactccttt aaatcccttg ataaaaaggg cttgttaaag
    42541 ttgatagggc tacggattcc catcaagttt gagatggcca aagcaaacat agaataacct
    42601 gcaaagtcaa agaagagctc cagaccaaaa gtatacataa ctgccaaggc atagagatta
    42661 aagaagccac ctgactgcaa ggctaaattc ttcagaggag gtagtaaggt ctctcctaaa
    42721 acatgagcta ggataaactt atacaaaaag ccccgcatga tatagcggac agattcatcc
    42781 agcatatcca tcaactcatc tcgctcagga atggcctgat aattttcatt aaatcgctta
    42841 aaacgatcga ttggaccact cgagaaagtt ggcatgaaga gaaggaaacg gaggaattcc
    42901 cagagggtaa aatccttaat cactccatct ctcagctcga tgataatccc aaccgaacga
    42961 aatgtcaggt aagaaattcc caagaaccca agcaaagact gcgttccatt gatagctggt
    43021 tgcaccttga caaagataat cggaagtagg gacagaaaac taactaagta gaagacccac
    43081 ttgccatcct tgctttttcg ataatgcttg tagaaaagca ggagcaatat ttcccagcaa
    43141 aggtaaatac ccaaggcagc tagttgattg gtctttccac ccaccaacat ggtgacaata
    43201 aagaagagac ttaccaacac ttcataccag gcaaagcgtt tcttgaaaaa gagacctata
    43261 aagatgggca aggttgcagc aatcacataa acaaaatact gaggattgcc atatggctct
    43321 aaatgaggaa gctgttgaaa aaactccatc atctcttatt cacctcgtta atcaatcctt
    43381 tgatgtcaat ctttccattt ggagttagtg gcaaactgtc tcggtaaagg aatttagatg
    43441 gcatcatgta ggacatcatg atgtctgtca ggtcttcctt gatggccttg gtaatatcga
    43501 tatctcgctc aaactgctca cgaacaccgt cttttaagat gacataagcc aatagatttt
    43561 gtaccttgtg gtccttgtta tagcgcggta ctgcgacagc agattcgata aagcgagact
    43621 tgttgaggtt ttgagagaca tcttctaact caatgcggta accgttaaac ttaatctgga
    43681 agtccatgcg tccgccgtag agaagcaagc cctcatctgt catggttccc acatcgcctg
    43741 tgtgataggc tggcagacct tcaaactcaa agaaggcttc tgctgttttt tcaggattgt
    43801 tcatataacc ttttgaaaca gctggcccag aaacaatgat ttctccctgt tcgccatttg
    43861 gcagtttatt tccttcctcg tcaatgataa aggttggaga atcagccttg gtatagccga
    43921 ttggtaggcg tttgagagtc gctaacatct cgtctgtaac agcaactgct gacagagcta
    43981 ctgtcgcttc tgttgggccg taagcattga tgatacgggc atttgggaaa cgctcgcgca
    44041 gtttttgagc tgttttgacc gtcaattctt caccatcaaa gtagaaatgc gtgattccag
    44101 gcattttctc actgttgaag tattcagaca acatggccat atctgcaaag gatggtgttg
    44161 atgtccagat agcgattggc aatgaaaaga tagccgcaaa gagttgctta aagtcctgag
    44221 tgatgactga aggaagagtg aaaagcgtac caccaagtgc caaggtcggt gcccaataca
    44281 tgacagacaa gtcaaaagaa taaggtggct gtgccagcat ttgcggacga ctcggtgtcg
    44341 caaattcctt atccgtaatc atccagtttg taaagctgag gagattatca tgtgaaatct
    44401 gcactccctt aggcttacca gtcgtaccag aagtaaagat aatgtagtaa ttatcatctc
    44461 ccttgactgg atgcgtgatt tcatagttgt tcccttgggc aaaggcttct tgaacctgag
    44521 ctagatttat cattggtgta gaaacctgct ccaagggaaa ggctgaaatg gcaataatca
    44581 agcttggctc tgctacttct aaaatagctg aaactcgctc caaggccgaa tggctatcaa
    44641 ttggaatgta ggcatgacct gacttagtca gcgctacaaa ggttgccaac atttcatatt
    44701 cttggccacc aaaaacaacc acaggagact tctcaggcaa gcctagttgg tcaatgactg
    44761 cagctaaact atccgaatca gcctttaaat cgccataagt gtgttcctgc cccaaaacat
    44821 tatagacagg atagctaggc tgtgtctgag caaaatgctc aatggtttca atcatatctg
    44881 ctattggttt atttgacaca atagggattc tccttcaagt taaaattcat tatagataaa
    44941 gcttccttga ccctgaccaa gatagctaaa gaagtaaagc agccctagaa agataagaaa
    45001 atacaaggct gtccgaccaa gaaagaggta caattctttt ctctgtttca tcaagaaaaa
    45061 ccattcattt ctgtaatttt tcgctaaaat aagagtgatt cttactagct tatttttcta
    45121 ccattgtacc actttatata gtatcttttc aattgtttac cgtatgtttc caatagattt
    45181 cagcttattt taaggattat gcggtttttc tatatctatt tcaaaagaaa aaagcctgag
    45241 attttcatct caagctctag caataatagt tattttctaa actaaaaaag tgagaataca
    45301 agcacggcca aggctgctcc gataacaggg cctacaacag gaatccaagc gtaagaccag
    45361 tctccgtctc ccttgtttgg aattggcaag atgctgtgca tgatacgagg tccaaggtca
    45421 cgagctgggt tcaaggcata acctgttgtc ccacctagtg atagaccgat accgacaatc
    45481 aaagttccca ctgcaaaggt tccgatacct gcctgaaagt cgtaaagacc caaagcaaag
    45541 attgtcaaca ccaaaacaaa agttccaagg atttcgctaa tcaagtttga tacagtatcc
    45601 ttgatggctg gtccagtact gaaggttgcc aggatattgc ctgcattttc ttctgcctca
    45661 tagtgaggtt tgaattgcaa ccaaaccaaa atctgaccca gcatggcccc tgcgaactgg
    45721 gctaagatat aaggcaaaac ggaagcccaa ggcaaaccac cttttaaggc cacaccgatg
    45781 gtcacagctg ggtttaaata agctggactg agcttgccag atacaaagac tgcaaccgca
    45841 actgcaatcc cccaacccat agtaatcaca atccaacctg agctattgct cttggtttta
    45901 ggaagaacca cacctgcaac aacaccattt cctagaagaa tcaggattaa agtccctaga
    45961 aattctccaa ataattcatt catcatcttt ctgtctccat taaaaagaag ggacgggcga
    46021 caaggaatgc tgccctccac ctcttttatt ttttcttaat tttttaattc tgctaaatcg
    46081 ttgttagcga gagctgcttc gacatcagca cggtaagttg ctttttcttc ttctgtccag
    46141 tcatagaatc gtcccatttc atccaaaatt ggctcaacga tactatccaa gctatcacgc
    46201 ataaagagca tgtgattggt acgacgaaga aggaagtcaa ctgggctaag agtcaactca
    46261 ttgcgcattg catagtgaag ggacaaggtg tctgccaagc taagacctgg cgcttgttcc
    46321 aagctgtgag caagtgcaaa gaccttcggt gcatttgaac cgtaaagatt tgctaggtaa
    46381 tgagcttcct tgctatccaa accacgtgaa actccaagtt gcgcaaaggc ttcgatttct
    46441 gaatccacat ttgctgggtt caattctcca cctgaaacag ggtaagtttt agaattgatc
    46501 aatttaaagc tacggtcaaa ttctgctttg aggatgtcaa ccacgcgctc catagctcct
    46561 tcagccatct tacggtagtc tgtgatttta ccaccagcaa gagtcaagag accattgtca
    46621 tcacggtcca agctagaccc acgagaaact gcagatggat ccaaatgttt ctcagatgtg
    46681 ctactttcaa gcttgctgac agcagactca acatcttcac gtgttttttc tttggagaga
    46741 taagattcaa cagtcgcaat caagttgtca aagctttcat cactgatggt accgttattt
    46801 ccaccattat agtcagaggc actgttccct gcaatcaatg gacgaagacc tgcccagctg
    46861 ctttcgatat catcaatggt gatgttggat tctgggaagc ggttgttgac aatgccaagt
    46921 agataatcta catcttcttg agttactttt ggatgctcca aatcacctgt gtagtctgta
    46981 tcagttgtac caaagtaagt cttgttttca cgtgggagaa caaagaccat acgaccgtca
    47041 cccaaacctg tgtcgaagta aactggctgt gaaaccttga ttttacttga atccactacc
    47101 aagtgaactc ccttagttgg gcgcatttgt gagaattgcg ttcccttatt agacaaatta
    47161 cgtactttat cactccaagg acctgttgta ttaataacca gacgggcctt gatttcaaat
    47221 acttggtctg tcaagagatc acgagctaca acacctgtaa tcttgccact ttcgtcaaag
    47281 aggaagcctt ctgccttcac gtggttggca atgagggcac cgtcttggtt ggcacgtttg
    47341 atgttttcaa tcacgagacg cgcatcgttg ttacggaagt caagatacac tccacctcct
    47401 accaagcctt ccttcttcaa gtttggctgg cgttccaaga cttctttctt gctcaaaacc
    47461 ttgttcgcag ttggtgtgtt gctcacacct gctaaaaggt cgtacaagtc catggctact
    47521 ttaagacgga agaggctaaa ggttgctcca tcttcatcgt aaactggtaa gagcattgga
    47581 tctggttttg gaatgtgtgg agcgatttgt tgaaccactg cacgttcaga aaccgtatct
    47641 gagaccactt ctacgtcgaa ttgtttgagg tagcgaagac ctccgtgaac caattttgtt
    47701 gaacgactag atgttccttc tgcaaagtct tgcatttcaa tcaaaccagt ctcaagaccg
    47761 ctagctgccg cctgcaaggc tacaccagct cctgtgattc ctccaccgat aatcaagagg
    47821 tccagggtgc gttcctgcat ttttttaatt gacaattcac gtgttttctt tgaaaattcc
    47881 atatttacac actttctaaa taaatcgctt tattctgcca gtattagtcg tctacttccg
    47941 caaagacttg agttgctttc acagccttct tccagccctt gtagagttgt tccttgcgag
    48001 attcattcat agatggctca aagagttctc ctgtctcgtt caagagtttc aactcgtcca
    48061 agtctttcca gtaccctact gataaacctg ctaggaaggc cgctcctaga gctgttgttt
    48121 ccaagttttt agcacgtgca atatcaatgc ctaaaatatc cgcctggaac tgcatgagga
    48181 agttgttcat ggctgcacca ccatccacct tcaatacttg aatggcagtc tgagtatcca
    48241 cttgcatggt gtcgatgatg tcacgcactt gataagcaat agattgcaaa gtcgccttga
    48301 taaagtcttc tttgcttgtt ccacgagtca aaccaaagac ggaaccacga gcattttggt
    48361 tccagtatgg agcgcctaga cctgtaaagg ctggaacgac ataaacttca tcgttgttgt
    48421 gagaatcacg agcgtatttt tcagattctg gtgaattttc aaccatgcga agaccgtcac
    48481 gaagccactg aatagcactt cctgcgatga agatagaacc ttccaaggca taataaacct
    48541 taccgttgat tccgtaacca atggttgtca agaggttgtt ttcagacaac tgcatctctt
    48601 ccccagtatt catgatgatg aaagagcctg ttccataagt attcttaacc atacctggct
    48661 caaaagccaa ctgtccaaag agggctgctt gttggtcccc agccatacct gagattggca
    48721 cctctccacc gtagaaatgg aatggagctg tcttgccgta gatttcggag ttagaacgaa
    48781 cttctggaag tatagccttc ggaatgttaa ggatttccaa aatctcatca tcccatttga
    48841 gttctttaat gttataaagc atggtacgag ctgcatttga gtagtcagtc acgtgagccg
    48901 caccgtcagt caatttccaa accaaccaag tatcgatagt accaaagagc aattcccctt
    48961 tttctgctcg ctcttgagcg ccttctacat gatccaaaat ccaacgaacc ttggtagcag
    49021 agaagtaagc atcaataatc aaaccagtct tttcatggaa tttttccaca taaccttggc
    49081 tttttagttg ctcagccaaa ggtgctgtct ggcgtgactg ccaaacgata gcattgtaga
    49141 taggaagtcc tgttttctta tcccagacaa ccgttgtttc acgttggttg gtaatcccga
    49201 ttgcctcgat ttgatttggc ttgacgccac tttcgatgaa agcacccgca ataactgact
    49261 gaacagagtt ccaaatttca ttggcattgt gctcaaccca acctgcctga gggaaaatct
    49321 gggtaaactc tttttgactc gagctaacct tttccccttt tttgttgaaa atgatggcac
    49381 gagaacttgt agttccctgg tcaatggcca tgatgtattt ttcttgtgac atgtactgtc
    49441 ctcctgataa agatcttgag gtgtttcctc tttacactta ctagtataca aaataaagcg
    49501 ctttcttgtt tatcaacttt ttttaatttt taaaaaaatg tgaggagatt gtgtgaattt
    49561 cacaaaaaag acctggatta accaagcctt ttaagtgaat aataactgac gaatccctgc
    49621 caaatcttcc atatccaagt cattttttaa ataatagact ggggtctgtt ccttcttatg
    49681 aatcgggtta ttggtaacca gcaaatcata atgccgacta gggtcataag cttcaatggt
    49741 aataaaacga ttgtattcca aataccgttt caaaatggct gccatccttg aaaagacaag
    49801 aaaaccagat gtcaaatcca gaccaatccg catatgctgg ccaccgtttt cagccatata
    49861 ttcgattaac tggagccatt cccagagaat cttcttatct aaatctgtcc cctgttgaaa
    49921 agcaggtaga gcatgaaaaa tctcctctgc tgtctcttta aaatcatgtt ccatcagtaa
    49981 atatggatga cgattctcta actggtactt gtagcgatcc tgtaaaatat agcccttata
    50041 gagatagact tgagcacaaa gctgactgag ttcataggtg acatggtcct ctgtataatc
    50101 ccccaatagt tcctccttct tcatttcttg aatcaattgc gtcagtaaat ctgccaactg
    50161 ccctccaaaa ccaagaatat attccatagt atgaagagga agaatgcgat gagataggag
    50221 aaaagagaag aatatcatca tctcaccatc ctcaactcct agtggaatat gttgcccagc
    50281 aaaaaaggac ggaacctttc tcagcaaacg aagatagaag atattgtcaa aataccctcg
    50341 cattttctct tctatgactt gaaactgaca agcattgacc cgaagacgtt gttgactgat
    50401 gtgagcccag agaaccaagt ccaatttctg ccccacagac aaacttgcac cgcagatttc
    50461 ctctaaattg gcaatctcct gttttctctc tggtttctgc atgtgacctt cccattcctg
    50521 actcgaccag acctttcgga aaagacagaa atagaaatag cgaatctgat gctctggacc
    50581 tcgccaacgt ccattttgga tggataaatc aaattctgac aaaatctgat ttaaaccagc
    50641 caagtgacga ccaagcgtag cctcgctaat caccaattct tgagccagct gatgggctaa
    50701 aaactgttgg tggtagagaa gataaaccaa aatctggtat ttaacagcac tctccaaaaa
    50761 caagctccga atatctctcc ccttggtagc tgcaccgata gacagacgca gattttcatc
    50821 ttctgagtga atagccagct ctaagccact atccaaagcc ttgtcattga gcagggtgac
    50881 atatttggtt agggttgctt ttgaaaatcc tgtttcttcc attacagcct tgacggtcgt
    50941 ctgagactct tgtaatagaa aggaaaggat tgaaaattga ccacactcgg ctttctccat
    51001 caaatctcct aaatacattt gttacccctt tcattttcta ccttaagcat agcataaatc
    51061 ttacaaatgc taaaataatc tgtttctctt tttattttca tgctgatttc ctggtccatt
    51121 atcctgaaaa tcagtaaaca cacggctccc cctttgggca tttttatgct aaaatagtag
    51181 ctatggataa aattattaaa actatatcag aaagcggagc ctttcgtgct tttgtccttg
    51241 atagcactga aaccgtccgc actgctcaag aaaaacatca aacccaagct agctcaactg
    51301 tagcgcttgg tcgaactctt atcgccagcc agattctcgc agccaatgaa aaaggaaata
    51361 ccaaacttac agttaaggtg ttgggatcta gctctctagg tgctattatc accgtcgctg
    51421 ataccaaggg gaacgtcaaa ggctacgttc aaaatcctgg tgttgacatc aaaaagactg
    51481 cgactggtga agtcctagtc ggaccttttg ttggaaatgg tcaattcctc gttatcacag
    51541 actacggtac tggaaatcct tacaactcta taactcccct catctctgga gaaatcggtg
    51601 aagaccttgc cttttacctt actgaaagcc aacaaacgcc ttcagcggtc ggcctcaatg
    51661 tccttttgga cgaggaagac aaggtcaaga ttgcaggtgg tttcctagtt caagtcttgc
    51721 caggagccaa gaaagaagag attgctcgct ttgaaaaacg catccaagaa atgccagcta
    51781 tctctactct tctcgaaagc gacgaccata tcgaagccct cctcaaggct atctacgggg
    51841 acgaagccta caagcgtctt tctgaagaag aaatccgttt ccaatgtgac tgtagccatg
    51901 aacgctttat gaacgctctt gccagccttc caagctcaga cttacaggaa atgaaagagg
    51961 aagaccacgg ggtagaaatc acttgtcaat tctgccaaac tacttacaac tttgatgaaa
    52021 aggacctgga ggaactcatt cgtgacaaat cttaatacac cttttatgat tggcaatgtt
    52081 gagattccca atcgtaccgt tttagcgcct atggctggcg tgaccaactc agcctttcgt
    52141 accatcgcaa aagagctcgg agctggactc gttgtaatgg aaatggtctc tgacaaggga
    52201 atccaataca acaacgaaaa aaccctgcac atgcttcata tcgatgaggg cgaaaaccct
    52261 gtctctatcc aactttttgg tagcgatgaa gacagcctag cacgcgcagc agaattcatc
    52321 caagaaaaca ccaagaccga tatcgtcgat atcaacatgg gctgccctgt caacaaaatc
    52381 gtgaagaacg aagctggtgc tatgtggctc aaggatcctg acaagatcta ctctatcatc
    52441 aacaaggtcc agtctgtcct tgatatccca cttactgtca aaatgcgtac cggctgggcg
    52501 gacccatctc ttgcagtaga aaatgctctc gctgctgaag ctgcaggtgt ttctgccctc
    52561 gccatgcatg gccgtacccg tgaacaaatg tatactggcc acgcagacct tgagaccctt
    52621 tacaaggttg cccaagctct aaccaagatt ccattcatcg ccaacggtga tatccgtact
    52681 gtccaagaag ccaagcaacg catcgaagaa gttggtgctg acgcagtcat gattggccga
    52741 gctgccatgg gaaatcctta cctcttcaac caaatcaacc attactttga aacaggagaa
    52801 atcctacctg atttgacctt tgaagacaag atgaagatcg cctacgaaca cttgaaacga
    52861 ttgattgacc tcaaaggaga aaacgtcgca gttcgtgaat tccgcggcct cgctcctcac
    52921 tatctccgtg gaacatctgg cgctgccaaa ctccgtggag ccatttcgca agctagcacc
    52981 ctggcagaga ttgaagccct cttgcaattg gagaaggctt aatagtttaa aacccgtaac
    53041 tctcttaaag agcctcttga atgccgccat tctttcctca tcaaaaaaac tccgtagtct
    53101 ctagcgctat aaatccagct agagattaca gagttatttt ttaatctaaa cttcctacaa
    53161 gggtatatta tctactgaaa ggatatggta tttgtaggga atactttaaa gagttcttac
    53221 aggaagtcag tattaactag ttatattagg tttagtttac ccattcacca ttggcattga
    53281 ctctatagct atctacagtt gtgttgactg caagggcacc tgagccattg acatagtacc
    53341 atttatctga tactttgaac cattggcttg ctttcatagc acctgatgct tcaagatagt
    53401 accaggtatc tccatctttc acccaatctg ttgccattga accgttagcg tttaggtagt
    53461 accatgaacc attgttttgg agccatcctg tcaccattga accattagcg tttaggtagt
    53521 accatgagcc attgttttgg agccatcctg tcgccatatc accattagcg tttagatagt
    53581 accatgaacc attgttttgg agccatcctg tcgccatagc gccattagcg ttgaggtagt
    53641 accatgaacc attgttttgg agccatcctg tcgccattga accattagcg tttaggtagt
    53701 accatgagcc attgttttgg agccatcctg ttgccattga accattagcg tttagatagt
    53761 accatgaacc attgttttgg agccatcctg tcgccatagc gccattgctg ttgaggtagt
    53821 accatgagcc attgttttgg agccatcctg tcgccattga accatcagta ttgtagaagt
    53881 accacatacc gttttcttgt ttccagcctg tttttggagt agatggttgt gctggttttt
    53941 cagtttttgg cggttgctgt tgagtcaagc gattatattc ttcttctgat ctacgagcat
    54001 agtcttcttc agcttgttga tcagctggtt tttctgcttt tggttgttca gctggattct
    54061 ctggttttgg agctggagct ggtttttctg gttgaggagc cggcgctggt tgtggttgtt
    54121 cagctggttt ttctttaact ttatcttctt ctgctgcttt tcgtttagct tcttctgctt
    54181 ttttacgatc tgtcttgatt ttttctaacc ttgtagcctc agctttttta ctctcaactt
    54241 ccgcttttgc ttgcttaact ttttcctcgt ttcgagatcc cttagcttcc tcttttacta
    54301 gttcaagctc cgctttttta acttccacat cggactcagc aatttcaagt tcaagcgttt
    54361 tgtaagtaat ggttgggtag ttacggtgat cttcttcttt ttgatccttg gcttttttct
    54421 tagcttcttc aaccttcttc tgagcttctg ctaccttttt ttctggtttc agggatgggt
    54481 ttggaagagt ttcttcacct acgctagaat ctgaagactt cgcatcattt tcttttttat
    54541 caggtgttgc ttgctctcca ggaactcctc gttttgcccg cccctttggt ttaccttgct
    54601 ctttagcatc tgctcttcgt ttagcttctt cttctgcttt tttacgttct gtcttgattt
    54661 cttctaacct tgtagcctca gctttttcac tcttaacttt ctcttctact tgcttaattg
    54721 tgccctcgtc tcgatgttcc ttagcttcct ctttcaatag ttcaagctcc gcttctttaa
    54781 ctttcacatc ggactcagca atttcaagtt cgattgtttt gtaagtattg gttgggtagt
    54841 tacggaaatc ttcttctttt tgatcctcgg cttttttctt agcttcttca accttcttct
    54901 gagcttctgc tactttttta gtaagttctg gttctttttt aaactgctca aaagctgcgt
    54961 ctaactctgc tttagctttt gacggcaact cagctttcga cttctcttct aataaaacat
    55021 taacttctaa ttcatacaaa tacttctttt taatttcgct caactttgtg attaagccga
    55081 cattttgggt atgtcgatct ttttccaaac tttttctgac cttctccaat atactttgat
    55141 aatgcgactt gacttcatca gcatacttct tcgatgtatc ttgcccacta gatgtaaccg
    55201 tggaggtatt cctaccacca accccttctg catggactac tcctcctaag aacaagctgg
    55261 caactgctac actagctact ccaatactaa atttacgaat tgaataatgt acttttcttt
    55321 cgctttttga tgcaaacatg tttatttcct tctatatttt ttctttaacc ttactatatc
    55381 acttcaatat gaactgtata taaatcgaat tttgtattta tctctttatg cttccatttg
    55441 ggattataac ttcatgtaat agcttttcct cttcagctat tcgtcgatac aaaataactg
    55501 cataaactgg aaaaactaaa acaaaagtca tataggcatg actcaacaag gtcaagccaa
    55561 tcaactcagg taaaatattt aaaaagtaat tagggtgttt tactgtccta aacaagatat
    55621 gatctacata tttgtgatta ttgacaagca taagcttcac tgtccaaata tctcccaaca
    55681 agtgaatcac caacatcaac atcagcatag aaaaaataag caagactaaa ccaaccatgc
    55741 ccatgccatc aaaaattgtc ttgtgcacca ttgcctcaac caagcaagag agataaaaaa
    55801 caatatgagc catagttaat cgttttgtat tctcaactcc aaattctttc ccgccattgc
    55861 taaggattcg tttctcattc tctatcgaac gctttaaaaa aatcaatcga ataaggaatg
    55921 ttactaaaat aatcgctaat accatattag tatcctttct tttatctaca caaagaataa
    55981 cacacttatg ttaaccctat atgaacttta ataaaaaact aatctgtcta caagctaatc
    56041 ttaaattcca taccgtgttc ataagggaga aaactataat ccatttgata gctttctaaa
    56101 atattattca caatgtaaag acccaaaccg gaactttcat ccttatctgt cacgatttga
    56161 gaatgataga atatatcaaa taaatgttct agttcttggt cacttagagg cgcacatgta
    56221 tttttgatag agagatagcc atcttgctcg gatatgatta cgcgcccatt tttatctgaa
    56281 tatttaattg cattactaat caggtttgtc aaaaccttat ccaatgccct aagactcatg
    56341 acgaccctgg tagcatctgt caattgattt tcaattgtaa cacctattga atgagctaat
    56401 tcttgataac gtgataaaat atctactaaa actggcttaa cagtcaaggt ctcacgacac
    56461 tctgtccact cttggaattt agaagactcc agtacttctt ccaataagtg gctcatctgg
    56521 tcaatcttat ttatactctt tgcaatatat tttggatgat ctttgtaatc tccaatatta
    56581 tgctgcatat tctctaggat aattctaaga ctggctaaag gggttttcaa ctcatgtgat
    56641 gctccgcgga caaaggaaac cttttgattt tgcaatttta caatttgctc attacgactt
    56701 tccaactcat gaataacctt caacaagtgc tcatacatac cattaatctg ttttccaact
    56761 tcaccaactt caccaacttc atctttccta ctttcatcaa aacgaatatt gtcatccaaa
    56821 tcttgcattt tactagtcac ttctgaaatg taaaaaagag gattgagcaa gcgtttagta
    56881 taaaaataag aaaaaacaaa ggaaaaagca attgtaacca aaaatgtata tgggagatac
    56941 aaaagcaaaa tattctttgc ttctttgtag acatccaccc cgtgaacaaa ttgcactgtt
    57001 acctgtttac catctgctgt actaacagac atatctaaca tcacgatata atttgatagc
    57061 tttccatctt taatatccaa gtcatgcaca agagggagtc gcttatctac aatatctctt
    57121 ttaaggtgag cagatatggt caaggacttt gaataagact ggagtaagct tggaatttca
    57181 tcagatgtct tagtctttaa atactccgta aacactcttg cgctctcatt aaacttttcc
    57241 ccctccagtt gaatataata aaaaggaaag gtcaaataaa tagctatatg aatgactaga
    57301 ccaacaccac ctagaattgc aaaacttctt aaaaaagact tggttaataa tttaggattt
    57361 tttatcataa gctaatctta tacccaacat ttttcacagt cacgatacaa tctaaagcta
    57421 attttttgcg aagagtacga atatagacat caacgaccct atcacaaggt aaatcagcta
    57481 catcttttga aatttcttcc aatatctgct ctctacttaa aacttgattt ttatgctgaa
    57541 tcaaacactt tagtaccagt aattcctttg gtttgagatc aatttcttca tttttataat
    57601 gtgctttgta agaggtaaaa tccactgtta catcctgata tcgccaaata tcctctatga
    57661 cgtagtaacg tctgataagc gccttaatac gcttgacaag cagtaccaaa gaaaaaggtt
    57721 ttgtcacatg atcatcagca tagagatcaa atacctgtga ctgagtagtt tcatctccca
    57781 gagcactcaa gacgatgaca ggaatatcgt gcttttgatt gatttctttt aacacttcaa
    57841 aaccactctt cctaggcatc attaaatcca gtaccatgag atggataggg agatcttgaa
    57901 atttttccaa gacctcttgt ccatccttag ccataatgac atgataaccc tcttctgtca
    57961 gaaatgctgc aattccttct ctaatcattt cctcgtcatc tgcaactaaa atgttcatct
    58021 ctctcccttt ctactaccag tctatcatct atgataatgg aatggatttc atttatccgc
    58081 tactttttgt caacaacaac gtcaatacaa gaaaatcatt catcttgcag cttatactct
    58141 tcgaaaatct cttcaaacca cgtcagctct atctacaacc tcaaagcaac ctgcggctag
    58201 ctgcctagtt tgctctttga ttttcattga gtttcactcc actgtgactt atggtcagat
    58261 gctgatactt ttattctttt atgcaatatc aaattttaac tggcctgctt tgacaccaat
    58321 cttaagtgtg ctgcctgcca ctaaatctcc cttaagaaga agttctgcca acttgtcctc
    58381 cacttctgtt tgcagggttc tgcgaagtgg gcgagctccc atctctgggt catatccttg
    58441 atttgctaac aatttcagag ctgaagcttg taatttcaag tcaatgcctt tttcagccaa
    58501 acttgccact aaaggcttga ccataatctt caccacttcc tgcatatggt cactagatag
    58561 gctatggaag accaccttct catcaatacg gttgatgaat tccggtctat aagctttttt
    58621 cagttcttca aacatgcgtt tttccatatt ttcctggtca aaacgaatat ccttagcccc
    58681 aaaaccaaca gtcttatcat cacgaagggc agtcgcacct aggttcgatg tcataatgat
    58741 aatggtattt gaaaaatcga ccttgcgtcc cttgctatct gtcaagacac cgtcatccag
    58801 aacctgcaag agaacattaa agatatctgg gtgagccttc tctacctcat caaagaggag
    58861 aacggaatag ggtttattgc gaaccttctc tgtcaactcc ccaccttctt cgtaacccac
    58921 atagcctgga ggagctccat ttagacggct ggctgcgaat ttctccatat actcactcat
    58981 atcaaagcgg ataagggctg attcgtcatc aaagagaact tctgccaaag ccttggccaa
    59041 ctcggtctta ccgacacctg tgggtcctag gaacataaag gaaccgatcg gacgcttgtg
    59101 actgcgaatt cctgactgat tacggcgaat ggcacgacta atacttgaaa cagcttgatc
    59161 ttgaccgatg acacgtttat ggagttctgc ttcaagattt aaatacttct tagcatccgt
    59221 ttgagtcagt ttttgaactg ggattcctga caagcgactc aaggtggtca aaatatcaga
    59281 ctctgtcacc aagtctttgt agacaggtac ttcctcttct tttgcgatta gctgggctgc
    59341 ctgtttccac ttgccatcca tcagggcctt gtcagctgga ctcaaatctg aatcgtctgc
    59401 ttttacatgc tttgccttat tttgcactgt tgctgctgct tcgtccaaga ggtcgatagc
    59461 agagtctggc aagtgacgac tggttaaata acgatgagcc atcttaaccg ctgtttcaac
    59521 cgcttcatct gtgatttgta cacggtgatg tttctcataa gtcgccttca aaccttgtaa
    59581 aatagtcata ctatctgcca cacttggttc ttcaatcgtc actttagcga aacgacgaga
    59641 aagagccgca tctttttcga tatgtttttg atattcttcc tgagtggtgg caccaaccgt
    59701 tctcaaagtt ccacgcgcca aggctggttt caagatattg gccgcatcca gagtcgaatc
    59761 aatcccgcta ccagaaccca tgatggtgtg gagttcatcg ataaagagga tgacttggcc
    59821 atcttcttca atatccttga tgatattatt catgcgttct tcaaagtcac cacggaagcg
    59881 tgtccctgca acgacattca tcaaatcaag ttctaacacg cgcatcttag ccatttccgc
    59941 aggcacgtca ccactagcaa tacgctgggc aagaccaagg gccagagctg ttttcccgac
    60001 accagcatcc ccaaccaaga cagggttgtt cttagtctta cggctcaaaa tttgaatcat
    60061 acgtgagatt tccttgtccc gaccgatgac tggttctaac ttgccagaac gcgcttgctc
    60121 tgtcaaatca tgcgtatagt cctcgagacc accactagga gtctgcggca tgcccatcat
    60181 attggccata gaattttgct tgtcagctac tgtacgatgg cgttggcgta aagccttgag
    60241 atcttcacga gtccagcctg cccgttcttc taaatttcga cgaagagcag caatcttgac
    60301 ctgatctttc ttgtcttcat aagaaaaacc agccctctcc aagatacgag tcgccaaggc
    60361 attgccatca tgcaaaatcg catagaggac gtgctctgtc cctagcacct tagcatggac
    60421 cactgacgct acatactctg cttcgtcaaa aagaacctgc aaacgacggg agaacggcaa
    60481 ttccgtaaag gtttcatcct ggctatagtc cgtttcagtc agttccaaag ccacctcttc
    60541 taaacggtcc atctcatacg gataatcatt taaagttgcc cctgctacac tataactgtg
    60601 attagacatg gcaatcaaca agtgccacga ctctagataa cgagctccaa aatgtccagc
    60661 aaccatgtag gcactttcga tacattcatt caatgctttt gaatagttca tcttacttcc
    60721 cttttctatc tacctcttgt atgacctgac gtagcatgtt tgctcgaaca actggagctt
    60781 cttctcctaa aacgcgatcc aaagctactg attctagcaa attcatctcc tgcttggtca
    60841 tcaattcctg ctcaaccaaa agctggagaa tatcctcata aatttcttga ctgactcgct
    60901 caccaatcga gtaaagcagc tcccggagca tttcatgatg actagaaaac tcaatccgtc
    60961 ctatacgaat gtagcctccg ccaccacgct tactttcaac caagtagcct ctactttccg
    61021 taaagcgtgt cttgatcacg tagttaatct gactaggaac aacctgaaag gtatctgcca
    61081 actgactccg ttgcaactcc acgataccag attgatctaa aatcgccttg atgtaggcct
    61141 caatatgatc cgatgtattt ttaaatctca taacaaaccc acctctctct ttaaaccttg
    61201 accttgtttg actattatac cgaaattttc tcatttttaa aagaaaaagg gcgctggtaa
    61261 aggataatct tcaccaactc cctatttttc tacttatcta agcctaattc tgccaagatt
    61321 tgacgtttgt aggcaatctt ttggacttcc ttgtcctcat cttcagacca atctagttta
    61381 atttctgaaa caatctgccc agggcgattt ttcaagatat agatgcggtc gctgagattg
    61441 agggcctcct caatactatg cgtgatgatc agggttgtta gctgcaactg cttgtgaatc
    61501 tcaagatacc aagcgtggag ttccatcttt gtcatctcat ccaaggcgct aaaggcctca
    61561 tctaagagaa agagcttgtg cccaaaaagg taggtccgga gtaaggctac acgctggcgc
    61621 atcccaccgc taagttcatg aggatacttg tctcttacag ctgtcagctg gaaggtcgca
    61681 agaattttat ccgctcggga aatagcttct gccttatcca ccttttgaat caagaggggc
    61741 agaatgatat ttccaagcac cgtcttgtgc tccaagagca gatccttttg caacatataa
    61801 ctcacgcgcc ccttgggatt ttcttcacca tcaaggacaa ttctccctga ctgaacttct
    61861 aaaatcccag cgattagatt aaagagggtg gtctttccaa caccacttgg gcctaggatg
    61921 gaaaccactt cgcctgaagt cacctgtagg ttgatatcct ctaaaatcct ctcctgacca
    61981 taggcataac tgacgtgctc tagtctaatt tctgtcatta tttcacaaat tcgttggtga
    62041 agcctttgtc tgtcaagtct tctttaagga taccattttc tttatcccat ttgtagaaag
    62101 cattccagcg agctgcgtca aattgacccc atttttcctt gtcgcttgcg tattcttttg
    62161 acaagtattt ttgagattcg atgacaaagt cacgtttttc cttgagttca ggtgcattct
    62221 tgatgagaat atctgcagct tcttctggat gttccatggc atattggtag ccttttttga
    62281 tggcttggat gactttgcga gcttcttctt tgttatcttt cagatagtcg ttgtttgcga
    62341 tgataactgg tgaatagtag tcaaactcct tgacatagtc tttcaagtac atgaagttag
    62401 catctacacc ttgagattta gcaaggatac catcccaacc gtagtaaatc caagcagtat
    62461 caaagacccc attggcaatc ggtgtgattg agtttgagtc gttatttggt actttttcaa
    62521 ccttctcaaa gtctccacct tgagattcta ccaaggtttt caacatagca agttcagttg
    62581 ggtcattcca tgtcccatat ttcttaccaa ccaagtcttt tggactgctt acattatcag
    62641 atttacgaga gatgattcct gatgtattgt gttcaacaat agctgcaacg gcagtgattc
    62701 ctgctccttt ttccaatttc ttagccatgt agtcttggaa atacactgca aatggtgcct
    62761 ttccgttgat aaccaagtca gaagaacttt cttctggtgg caatttcaaa tcaacatcca
    62821 ctccagcttc tttgaaataa cctttttcct tggcaacata aagccctgtg tggttggtat
    62881 ttggtgtcca gtctaggata aagtcaacct tcttaagttc tgcctctttg ttgtctttag
    62941 aagcagttcc ttgaccacag gccacaagca caacagctac aagagctgtt acaagcgtta
    63001 aaaacacttt ccatgttttc ttcattttga tttcctcttt tctttttcag aaacattcta
    63061 attctacgaa cgtttccatt taataacata tttttcacta atatcgacca acttcatacc
    63121 caaaaggctg ataatcgata ccagaataat aatagcgaac atggtatcat actgaaacaa
    63181 tttcttggac tgaatcatat aaacaccaag cccctcaaag cctcccaacc actcagatac
    63241 cactgttgtg ataaaagcgt aggagacact gaccctcaga ccagcataaa agtagggcag
    63301 gctgactgga attttaaaat gccacaggat ttgccaaggc ttggcccgca tcagactaaa
    63361 caaggtcagc atatccttgt cgcaatgcct aaaaccgtcc aaaatgctga cgatgatggg
    63421 aaaggttgtc gttaagataa tcaagacaat cttgggcaaa atcccataac ctagccacaa
    63481 gaccaggata ggagctatgg caatggtcgg aatggtctga atgaccacca tcatagggta
    63541 aatcaggtca ttgagccaag tcaaactatc catgagtaca gccataagac aggcaatcaa
    63601 aactcccaaa atcagcccca gtaaagccac tctcaaggtc gcccagctat ggtgccagag
    63661 aaattctctg tcacgaacaa agggctggag aatttcaaga ggtgtcggca ggataaactt
    63721 ggggagaagt ttaagaaaac ctgctaactg ccagattgac aagactccga gaaagcccaa
    63781 tagactaatg tgtcgtctca gtatacttct caagtttctc atcaatactt aaaatctctc
    63841 ctacatttat tttgacattg gcaaagacat tgtctgcccc ctgccctgcc acttccagcg
    63901 cttcttttag aatgcgcata agctcatcaa actccccttc caagaccgtt tcaaatggtg
    63961 tcactaccat cgtcacttct tgagtttgca gataagcaat gacctgatca ataacagcta
    64021 tccgatcaat cccctgtgct aggggtaaaa cttgcaaggc aatgcttgct ttcataagaa
    64081 cctcctcttc tcgttttcct ttttaaaaaa agaaaaacct ttaccataca tggaaaaggt
    64141 gcaaaattag actaagtatc gttccctccg ctggcattac ccagatcagg tgcggtcgaa
    64201 gtttaacact tcctctcaga ctgtacacag actcccatat attatatgta tatatgatag
    64261 cgcaaaataa gaaaaatgtc aaggaaactg cttacagaaa agagcaggaa atttctccta
    64321 ctccaatttt ctatactcgt tctccatcac tgtttactct gtagccatcc accgtcgtat
    64381 taacagctaa ggcgcctgaa gagtaagaat aataccactt accagagacc tggtaccagc
    64441 ctgttttcat tgcccctgaa ctacccagat aataccacga gccattctcc tttaaccatc
    64501 ctgtctgcat ttcaccagat aatttcaaat aataccagct agaaccatct ttcagccatc
    64561 ctgttgattt ataaccattt gatttgaaat gataccaact accatttatt ttcttccaac
    64621 cgacagttga actattattg gatccatagc tagagatatt tcctgattga ttctgataat
    64681 tttgtgaaga agtcactccc tctagctgta tatagcgacg acttccgtta taagccgtat
    64741 aactcaacca cttgtagccg tctttttcaa gtatctgatc ataatgaacc ttctccccag
    64801 gataatagta atcaatcaca gttgcactaa ctaggggttc agttttgata gcagacttgg
    64861 tcttaaaata atgagttcca cctgttgaag aaagaacaga atgacctaga ggatttttat
    64921 tcacagcctc caattgaaca taacgatagc ttccattata ggcagtataa ctcaaccact
    64981 tgtagccgtc tttttcgaga atttgatcat aatgaacctt ctctccagga taatagtaat
    65041 caatcacagt tgcactaact aggggttcag ttttaatagc agacttggtc ttaaaataat
    65101 gagttccgcc tgttgaagtt gaggattgac tattcccaac actgccacca tccaaatctt
    65161 taaaatgaat aaatcctgtc atcgtgtttg cttttataat tcgtttatta taggattctg
    65221 tataaccata gttatattcc tcaatctcaa tctgatctcc cattacattt gacacccagg
    65281 caacatgacc ataagttcct gcagtagacc aagtaatgga accaatcgtc ggtgtattat
    65341 ctacacgata accttcccga cgagcacgat gtccccattc attcgcattt ccataagctg
    65401 ccggcatttc aaaaccattg acattactca aacgaaaggc tacaaaagaa gtacactgac
    65461 gagaatacat gcgccactga tcaatttcct ggctcccatt tttataataa gcaggataat
    65521 catccccacg cgcaatcgat ccatttcctc tggaataggc atagacacta tcaccagctg
    65581 ctagcatcaa tcctactact aaaaaaggaa caagcttttt aactgacatc ttcaagtaat
    65641 aacttgttcc tcttgctata aacggtaaaa ttttcattct tcctccttga aaaataatat
    65701 aaagcgatga ttgccttcac ctaaattatt cggagaaaaa agaaaaagtg agaaaatttc
    65761 tcactttagt caagataatg aatcaaattc ttttctgtaa gaatatctga gtaagtaaag
    65821 gattcaacgt aaacattaac ttgtttatct ccttccacat ccccaaactc cacaataaat
    65881 agggatggat aaacttcaat tagcttaccc aatctatttt tttggcgctt acgaccattc
    65941 tccaaagtca tttctacgac ttgtccctca tgtgccttga tttcttcttt gatttttttc
    66001 atcttggcta catctgtaaa tgcatctgac atcttatgcc tccctctttg agatacttga
    66061 aaatttattg tattcttttt ggaaaatcaa ttccactgtt ccacgagctc cactacggtt
    66121 tttctcgata ataacttcca ccttattatt tgggatacct tcctcttctt caccaccacg
    66181 ttcatagtag tcatcgcgat agagaaaagc tacgatatca gcgtcctgct caatagaccc
    66241 agattcacga atatcagaca agaccggtct cttgtcctga cgttgttcta caccacgaga
    66301 aagctgactc agagcgatta ctggaacctt caattccttg gctagtattt tcaactgacg
    66361 agaaatttca gaaacttctt gttgacgatt ttctcgacca gttcccgtga taagttgcaa
    66421 atagtctatc aaaatcaaac caagatttcc agtttcttga gccaatttac gagaacgaga
    66481 acgaatctct gtaatccgaa tacctggcgt atcatcgata tagatactgg cgttagctag
    66541 attaccctga gcaatagtat atttttgcca ctcctcatct gtcaattgcc ctgtacggat
    66601 agaatgtgac tccactaagc cttctgcagc taacatacga tctaccaagc tttccgcacc
    66661 catttcgagt gaaaaaatag caaccgtttt gtccaactta gtcccaatgt tctgagcgat
    66721 attcaaggca aatgctgtct taccaactgc tggacgagct gctaagataa tcaactcctc
    66781 ctcatgaagt cctgttgtca tatgatccaa atcacgataa cctgtcgcaa tacctgtaat
    66841 atcggtcgtt tgttgcgagc gagcttccag atttccaaag ttgagattca acacatctcg
    66901 aatgttctta aacccgcttc gatttgcatt ttcactgaca tcaatcagcc ctttttctgc
    66961 ctgagcaata atttcatcag ctggttgtga cgcttcgtaa gcttggttga cagactctgt
    67021 caacttggca attaaacgac gtagcattgc tttttctgca acaatcttag cataatactc
    67081 cgcattagca gaagttggca cagaattgac aatctcaacc aagtaagaca agccaccaat
    67141 attctgtaaa tcaccttgat tatcaaggat agtacgaacc gttgttgcat ctatggcatc
    67201 accacgatcg gataaatcga ccatggcttg gaaaatcaaa cgatgggcat acttaaaaaa
    67261 gtcccgagac tcaatgtatt ctcgcacaaa aacaagttta ctctcatcaa taaagatagc
    67321 ccctaaaacg gattgctcag ctaagatatc ttgaggttgt actcgtaact cttctacttc
    67381 tgccatcaga cttcccttcc ttttacaatc ttgtcaagaa ggtgtaaact tatccttctt
    67441 tcacacgaag attgattaca cttgtgatat cttgatagat tttcactggc acatcaatca
    67501 aaccaaccgc tcgaatcgga gcttgtactt gaatatgacg tttatcaatc ttaattccaa
    67561 attgcttttg caattcttct gcaatcttct tattggtaat agaaccaaag gtacgaccat
    67621 ctggaccaac tttttcaaca aattctacaa cagtttcttc tgcttcaagt tgtgctttaa
    67681 ttgcttttcc ttctgcaatc atctcagcgt gagctttttc ttccgatttt tgtttaccac
    67741 gaagttcacc tacagcttga gcagtcgctt ctttggctag attctttttg ataagaaagt
    67801 tttgcgcata ccctgttggt acttccttaa tttcgccttt tttacctttt cctttaacat
    67861 ctgctaaaaa gattactttc attcttcttt ctccttttcc tttatttcat ttaatacaat
    67921 ttctgtcagt ttttcacctg cttttgacaa ggttacatct ttaatttgag ctgctgccaa
    67981 attaaagtgg cctccaccgc ctaactcttc cataatccgt tgtacattca gtttactacg
    68041 acttcgagct gagatagaga taaatccttg tgtattcttc gcaagaacaa aactcgcttc
    68101 aatacctgac atggctaaca tggcatctgc tgccttacta ataacaactg tatcatagca
    68161 tttcgtgtcc ttagcctctg ctattagtac atctgaacct aatttacgcc cctgtaaaat
    68221 aagttcattg acctcacgat attcttcaaa atctgtcgca gcgatttcct ggatagcaat
    68281 actatcactt ccgcgcgttc tgagatagct agcaacatca aatgtccgac tagttactcg
    68341 cgaggtgaaa tttttagtat ccaacatcat accagccatc aaaacacttg cttgcatacg
    68401 actcaaacga tttttcttag aattctggaa ctgaatcaat tccgttacca actcactggc
    68461 actacttgca ccactttcga tataagtaat gaccgcatta tctggaaaat cctgatccct
    68521 tctatggtgg tcaataacaa tggtttgggt aaataaatca taaaattctt ttgataatgt
    68581 taaggctgtc tttgaatggt ctacaagaat caacaaagaa cgattggtca ccatccccat
    68641 tgcatcctta acagacaaca acttcgtaac tccttctttt tctatgaatg aaacagctcg
    68701 ttcaatatct ggagacattt gttcttcatc ataaagagca tagctatttt caatcacatt
    68761 gctggcgaac aactgcatac ctacagcaga gcccaaagca tccatgtcta aatttttgtg
    68821 accgactaca aaaacctgat ctacactccg aatcttatct gaaatagctg tcatcatagc
    68881 gcgcgtacga gtccgtgtac gcttgattga agcagcagac ccaccaccaa aataaactgg
    68941 atttttcgtt tcgtcgtttt ccttaacaac cacctggtcg ccaccacgta cttcagccaa
    69001 gttcaaattg agcaaagcaa ctttccctat ctcatcatga tttccatcgc cataagaaaa
    69061 tcccatactt aaggtcaagg gcaactgtct ctgtttcgac tcttctctga aagcatcaat
    69121 aacagaaaat ttatcattca tcaagccctc aagcaccgtg tagtcagtaa atagataaaa
    69181 tcgatccata cttacccgac gagaaaacat catgtatttt tctgaaaact ctgatataaa
    69241 attagctaca aaactattga tttgactaat atctgactca gaagttgcat cctccaaatc
    69301 atcataatta tccacagaga caatcccaat cactggtcga cttgttacca attcatctgt
    69361 tatggcttgt tccctggata catctacaaa atacaaaaca ccggaagaag catccatatg
    69421 aacagcataa cgcttctcac caagcttggc ataagtagac ggatttccta ctgaagcctt
    69481 aataatcgtt tgaacagctt ctaaatcaaa atcaccatct tccttggtca aaatcaattc
    69541 agcataggga ttaaaccact caacctctcc agaagataaa ttcaatttca taacacctac
    69601 aggcatctgt tccaatagag atgtcaaact ttcttccgct tggtggttta catactgtat
    69661 ctgttctaca tcactccttg tataatgcac tctcagtttc ttaaataaaa aaacatagcc
    69721 tcctacaaaa agaaacaaaa ttaaaaccgt caacagatta ttattaacaa aaataatgaa
    69781 agtggataag actccaaacg caatcaatcc tactagaata ggaaaaattg gacttacata
    69841 aaattttttc attcaaaacc tcttggcacc cattatacca taatacccct caaaaagcga
    69901 ctttttaaaa gtgtaatcag taattctatc aattataaga aaaaggtagt ttacaattca
    69961 gtaaacctac ctttacacat attgaaatta agattcttta acctctaaca aaccaatttc
    70021 gccatcctca cgacgataaa tcacattggt tgtctgatct tcaacatcca catagataaa
    70081 gaaatcatgc cccaataaat ccatttgtag aattgcttct tccaaatcca ttggttttaa
    70141 atcaatttgt tttgaacgaa caactttaga ctggacaaca tttgaatctt ccaccaaagc
    70201 atctgtaaat aattgaccag ttgctacctt atttttattt ttacgctcga tttttgtttt
    70261 atttttacga atctgacgtt caattttatc agttacaagg tcaattgaac catacatatc
    70321 ttgagataca tcttctgcgc ggagagtaat agatccaagc ggaatcgtta cttccacttt
    70381 agccgttttt tcacgataaa cttttaagtt aattcgggca tccaactctt gttctggttg
    70441 gaagtacttt tcgatctttt cgagtttaga aactacataa tcacgaattg cttctgttac
    70501 ttctaggttt tcaccacgga tactatattt aatcatatga gtaccttctt tctaaacatt
    70561 tttgttttta tgattttatt ataacgcttt cattctattt ttgcaaattt tttcctcatc
    70621 ttacaaggga aaatgttttt acatccttag caccagcttc ttccaacagt ttcttaacac
    70681 gatttatagt tgctcctgta gtatagatat catctataag taggattttt ttaggaatag
    70741 tgactccact tttaataaag aaaggaagtt ctgtccccaa gcgctctgaa cgatttttag
    70801 aagaactggc tctctcttct cttttctcta ataaatccag atactcaaaa cctgctgcct
    70861 ctaccaagcc ctcaacctga ttaaatcctc tattagcata tctatcagga cttaggggaa
    70921 ttacaacaaa ttgatactct ttgtactttt tcaactcctc acttaaaaat gaagcgaaaa
    70981 cttttcttaa caggaagtct ccatcaaact tataccgact gaaaaaatcc ttcatagctt
    71041 gattgtaagt aaaaatcgct ctatgactga ctccaactcc ttctttacac caaagttgac
    71101 aatcttgaca ctttgttgac aactctgttt tcatacaatt tggacagttc tcttccccaa
    71161 ttctttcaaa agtagaatca cagtctgaac aaagacaaga gtcatcattc ctcagaagta
    71221 agagactact aaaagttaaa acagtcttca tagtctgccc acataacaag cacttcatag
    71281 accagcctcc ttattcatca tctgaatttc cttaatcgcc ttcttgattg aagcatttaa
    71341 cccatcatgg aagaaaagca aatctcctgt cggtctatcc atgcttcgtc caactcgtcc
    71401 accaatctga atcaaactag acttggtaaa caaacgatga ttggcctcta ctacgaaaac
    71461 atccacacaa gggaaggtaa ctccacgctc caagattgtc gtactgataa gtattgtcag
    71521 ttctccatct cgaaaagctt gtacttgctc taatcgatcc tctgttacag aagatacaaa
    71581 gccaattttc tcatttggaa attgctcctg taaaatttct tctaactgct cccctttctt
    71641 aatttctgaa gcaaaaatga gtaacggata agctgtcttt ctctgcttct caatatagga
    71701 ctttaacttt ggtgacaaac gattcttgtc taagtagcga ttaaaatccg ataaccaaat
    71761 tggttttgga ataatcaacg gatttccatg aaaccgtctc ggtaaattca gtctttttag
    71821 ttctcctaaa cggacctttt tatctaactc attggtcgaa gtcgctgtta aaaagattct
    71881 caatccattc tcctttacac tattcttgac agcgtggtaa agcattggat tatcaacata
    71941 aggaaaagca tctacttcat ccactatcag caaatcaaaa gcttgataaa acttcaataa
    72001 ctgatgggtt gttgcaacaa ctagtggtgt tcgaaaataa ggttccgatt ctccatgtag
    72061 caaagctatc ccgcaagaaa aatcctgttg caggcgcttg tacagctcca aacaaacatc
    72121 tatgcgagga ctagccaaac acactgcacc acccgcattg atcactttag ccactacttg
    72181 ataaatcatt tctgtctttc cagctcctgt taccgcatga actaaggttg gcttttgctt
    72241 gtctactgct tgaagcagtc cctctgacac cttctcttga aaaggagtta attggccgcg
    72301 ccatttgaga acatcttgct tcggaaaatc ctcctgcgga aaatagtata aagtttgatc
    72361 acttctgact cgcttcatca gcaagcactc tcgacaatag taagcaccga tgggcaaata
    72421 ccattcttct agaatagtac tattacaacg ttgacagaaa agtttcccct tctcctttct
    72481 cattgctgga agtttctccg ccaactgacg ttcttcttct gttaattcat tctcagtaaa
    72541 taaacgaccg agataatcta aatttacttt catacttctt tattcgtaaa aactagcact
    72601 ttagatgatt ttttagtaca attaaatcat ggaatttaga acaattaaag aggacgacca
    72661 agtccaagaa gaaatcaaaa aatctcgctt tatctgccat gccaagcgtg tttatagcga
    72721 agaagaggct cgtgacttca ttactgccat caaaaaagaa cactacaaag cgacacataa
    72781 ctgctctgcc tttattattg gagaacgtag tgaaattaaa cgtacaagtg atgatggtga
    72841 gcctagtggt actgctggtg ttcccatgct tggggtacta gaaaatcaca atctcaccaa
    72901 tgtctgtgtg gtcgtgacac gctactttgg tggtattaaa ctaggcgctg gaggactaat
    72961 tcgtgcttac gccggcagtg tcgccttagc tgtcaaagaa attggtatta ttgaaataaa
    73021 agaacaggct ggcattgcta ttcaaatgtc ttatgctcag taccaagagt acagtaactt
    73081 ccttaaagaa catgatctca tggagctgga tacaaacttt acagatcaag tcgatacgat
    73141 gatttatgtt gataaagaag aaaaagaaac tattaaagct gcacttgtgg agttttttaa
    73201 tggaaaagtc actttaactg accaaggttt acgagaggtt gaagttcctg taaacttagt
    73261 gtaaacaatg aataatacag cgtttcgttg acattctcac aactacttta gcgagcaaaa
    73321 taaaaagatg cgtaccaaaa tatactagaa aatgaagcaa ttcaaacgaa acctgatatc
    73381 gttttccttc acacctattt actagaatta gctgaacgca atcacttgaa aattaatgac
    73441 tttgatctat gatatataga aatcctatcg cttcgatagg atttctatat tttacaaatg
    73501 gtatggatag cgttatacta aagatatctt atacaaagag gtattcatat gtctatttat
    73561 aacaacatta ctgaattaat cggtcaaaca ccgattgtta aacttaacaa catcgtgcca
    73621 gaaggtgctg cagacgtcta tataaagctt gaagcattta atcctggttc atctgtaaaa
    73681 gaccgtattg cccttagcat gattgaaaaa gctgaacaag atggtattct gaaacctggt
    73741 tctactattg ttgaagcaac aagtggaaac actggtattg gactttcatg ggtaggtgct
    73801 gctaaagggt ataaagtcgt catcgttatg cctgaaacta tgagtgtaga acgacgtaaa
    73861 attatccaag cttatggtgc tgaactcgtc ctaactcctg gtagcgaggg aatgaaaggt
    73921 gctattgcta aggctcaaga aatcgctgct gaacgtgatg gtttccttcc tcttcaattt
    73981 gacaatccag ctaatccaga agtacacgaa agaacaacag gagctgagat actagctgct
    74041 ttcggtaaag atggattaga tgcctttgtt gctggagtag gtaccggtgg aacgatttct
    74101 ggtgtttctc atgcactcaa atcagaaaat tctaacattc aagtttttgc agtagaagca
    74161 gatgaatctg ctattctatc tggtgaaaaa cctggtcctc acaaaattca aggtatctca
    74221 gctggattta ttcctgatac acttgatact aaagcctatg atggtatcgt tcgtgtaaca
    74281 tcagatgacg ctcttgcact cggacgtgaa attggtggaa aagaaggctt ccttgtaggg
    74341 atttcctcag ctgcagctat ctacggagcc atcgaggttg ccaaaaaatt aggtacaggt
    74401 aaaaaagtcc ttgccctagc accagataac ggtgaacgtt atctctctac agcactttat
    74461 gaattgtaac cgtccaataa cgaagtatat tgaaaaatct ccagactaga gaactcacgg
    74521 atagttccta atctggagat ttcttatttg cacttttctt gtacaacttt agtccatggt
    74581 aaataagcct ctaaaacctc tttgtttacg agagtttcca cgtttggaag acattctaga
    74641 agataggata gatatttctc actatttata atggattgaa ataagatatg aacaaatcga
    74701 ttaggacatg atggtaaagc gtaatccctt gtttctcagc tttcccagac aaaaaagtcc
    74761 aatagtaagt cagctgacta tcactctcta gcaccctata agaagtttca tccgcatgaa
    74821 gtaaggactg cgtcaatagt ctctctcgca agaggttata aaggggctcc aaatagtatt
    74881 gactcgcctt gatatgccaa ttagcaattt ccttacgtgt gattggtaaa cccatcttag
    74941 cccaatcttc ttcttggcga taattgggta ccttcagatt aaacttctga tggatggtgt
    75001 gagcgataat agaagctgag ccaaggctat gcgccaaagg ggctttagga ataggagctt
    75061 tcacaagctt atccagatga ttatctttta ctcgttatgg acaatgctat atggcataaa
    75121 tcaagtacct taaagattcc gactaatatt ggttttacct ttattcctcc atacacacca
    75181 gagatgaacc ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataaa
    75241 gcctttcaaa ctttggaaga tgtcatgaat caactccaag atgttataca aggactggag
    75301 aaggaggtga taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaaacaga
    75361 tgagtataaa aagaaagtcc tcatttcaat agaaatcacg actttctgat ggatttatag
    75421 taaaatgaaa taagaacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt
    75481 tttaaaagca gaggtgtact attctagttt caatctacta tatttttgga gtgatagaaa
    75541 agcccttcat aagctagtct acttgttcag gtgcgagagc tttgacatcc ttttctgtac
    75601 ttagccaagt cagttttccg ttctcaaagc gtttatatag tagccaaaat ccttgaccat
    75661 cccagtaaag ggctttaaag cggtctttac gtccaccaca aaagagaaag acttgaccgg
    75721 agaaagaatc caattcaaag tgggttttaa ctacataggc taatgagtct attccctgcc
    75781 tcatatctgt cttgccacaa acaaggtgaa cttgacctaa atcacttagt tgaattatca
    75841 tagtacaata cctttcctcc gataattatt ttttatctag tatactggaa gttggggaat
    75901 taggatagat accttgttat gacgcgctta ctatgaattt taagtatagt ctcctaaatg
    75961 cacttagccc ttattatagg gctttttgtt ttaattattc taatcgagtg agactgggga
    76021 aaaaacaatt tcaggaaaaa tctaagccct atacaaaaaa ggaagcaatt tgcttccttt
    76081 ctattattag ttattcaagg ctgctgccat tgtagctgca acttcagctt cgaagtcgtt
    76141 tgcagctttc tcgatacctt caccaacttc aaagcgagca aactcaacta ccgaagcgtt
    76201 aactgattca aggtatgctt caactgtctt gctgtcatcc atgatgtaaa cttgtgcaag
    76261 aagtgtgtaa gcttggtcaa ctttagtgtt atcaagcatg aagcgatcca ttttacctgg
    76321 aataattttg tcccagattt tttctggttt gccttctgca gccaattcag ctttgatgtc
    76381 agcttcagct tgagcaataa catcatcagt taattgagct tttgatccat acttcaagtg
    76441 tggaagagct ggtttattaa ccattgcacg gctttcgttg tcttggtcga taacgtgatt
    76501 caattgtgcc aactcatctt taacgaattg ctcatccaat tctttgtaag aaagaactgt
    76561 tggtttcatc gctgcgatgt gcattgacaa ttgtttagca agtgcttcgt ctccaccttc
    76621 aacaactgaa ataacaccga tacgtccacc gttatgttgg tatgctccaa agtgttgtgc
    76681 gtctgttttt tcaatcaatg caaagcgacg gaatgagatt ttctctccga tagttgctgt
    76741 tgcagatacg tatgcagctt caagagtttc acctgaaggc attatcaaag caagagcttc
    76801 ttcattgtta gcaggttttc cttcagcaat gactttagct gtagtattta ccaattcaac
    76861 gaattgagcg ttttttgcaa cgaagtcagt ttcagcgttt acttcaataa ctgctgcaac
    76921 attaccgtta acataaacac cagtcaaacc ttctgcagca acacggtcag ctttcttagc
    76981 tgccttagcc ataccttttt cacgaagcaa ttcaatcgct ttttcgatgt caccgtctgt
    77041 ttctacaagc gcttttttag cgtccataac accggcacca gatttttcac gcaactcttt
    77101 tacaagttta gctgtaattt ctgccatttt aattctccta tattttttga aaataggaga
    77161 gccgggctaa gccccgccct cctaggtaat tactatttgt ataaattaag cgttgtcacc
    77221 ttctacaact tcaacgattt cttcaattga atctgcttga gtttctgaag ctgcaaattc
    77281 tgcttcaact gctactgcat cctcaccttg acgtccttcg ataatagcgt cagccaattt
    77341 agctgtgatc aatttaacag cacggatagc gtcatcgtta gctgggatga ttacatcgat
    77401 atcatctgga tcagtattgg tgtcaaccat cgctacaact gggattccca attttttagc
    77461 ttctttaaca gcgatttgct ctttatgtgg gtcaactacg tacatcacat ctgggatacg
    77521 aggcatatct tcgataccac ccaagaattt ttcaagacgc gcacgttgtt tgttaagaag
    77581 tgcaacttct ttcttaggaa gaacttcgaa agttccatct tcttccatac gtttaatttc
    77641 tttcaaacga gcgatacgtt tttggattgt tccccagttt gtaagagttc cacccaacca
    77701 acggtggttg atgaagtatt gacctgaacg tactgcttct tcagcaactg catcagctgc
    77761 ttgtttctta gtaccaacga acaatacaac tgcatcgtta gctgctgcat cacgcatgaa
    77821 gtcgtatgct tggtcagcgt attttacagt ttgttgcaag tcgataacgt ggattccgtt
    77881 acgttcagta aagatgtact tagccatctt aggattccag cgacgagttt ggtgaccaaa
    77941 gtgtacacca gcctcaagaa gttgtttcat tgaaattact gccatgagta tttctccttt
    78001 ttgttttttt cctcttcttg acttcaactc gcaaaacgac ccaagggcaa ctgcttcaca
    78061 attcatcaag aatgagtatt atcgttcaca cgacaatttt cattctacca tacttttcca
    78121 atattttcaa gctattttga tataattttt aatagggatt gtcacggtaa aacctgaaaa
    78181 gagggctcta ttcgagtccc tctgtaaatt aatctgcata aatatatgta acaaaacctt
    78241 cagaagttgt tgttggattg aaccatccac ggtgatttcc aattgtacga ttacctgcat
    78301 aatttgattc tgatacttgg atacgtgttg ttgattcaac agctgtaaca accgctacgt
    78361 gaccatatcc accatcattc caacatgcaa ttgctccaac ttgaggtgtt gaacctgtac
    78421 ggaaacctgc tgctgctgca cttgtagccc actgtgctcc attaccccag tagtctccag
    78481 cccaaggtgc caatgttttt actccccatg tacattctcc aattggataa cttgaagcgt
    78541 ttgtactgta tgttggacga acttttgcac ggacaggtgc tgctgcagat tcagatactg
    78601 cttgcacttg agctgttaag ttagtgtttg ctgaagcaag tactgattgt tgttggctag
    78661 ctcgtttttc tttataagct gcttctgcta ccgcagctgc acgagcctct gcctcagctg
    78721 ctgctttttg ctctaataga cttgcttttt ccccttcagc tgtcgctttc tcagcagcaa
    78781 gacttaattc agcagctttt agttctgcct gtttcgtagt caatgcttga gcatcatcag
    78841 ccaatttttg ttgattagca attacagtat tgatagcatc attatttgct acttgttttt
    78901 cagaaatagc ttttttatct gccttttgtt gttctaacat tttgttgttt gcagatacga
    78961 tttcactcat tgcagcaaca cgtgaaatag cttctgtaat tgattttgag tttacaatgg
    79021 tattgatata gctagttacg gctccatttg tttgagcact acgagcttgt ttttccaacg
    79081 attggttacg agaaacaatg tttttagaaa gttctgtaat ctcaccctcg agtttcttag
    79141 attctgcttg taatctatca ttttcagctt gcaagttaga ctgctcagct tgaatagctg
    79201 atacttgctc ctgaatttgg tcaacttgtt tttgggcttc ttgttgttgt gctgttaagt
    79261 tactaatttt attatcttga gcagcaattt tgtcatcagt cgtttctgca tgcgcagttg
    79321 ttaaaacagc tacttgagaa accattactg tacttaataa aagtgacgct aagatttttt
    79381 tcttcatatt acgtagatac tccttctttt aaagacaata ctagtatacc aaaaaaaggc
    79441 ctaataaata ttacgccttt gttacattta tatgtcgttc ttatagataa tatttttcaa
    79501 aaataaattg aaaaacagta atccataaaa aatttaaaat catcgtcggt accaagctat
    79561 agactataaa aatcgacatg ctatctactg tcaaccctac cataaaagcc aaaagataac
    79621 tacccatttc aaacaggaat gtcagcacaa tcatagctag cattcttgtc caacgattca
    79681 acaaaataac actattcaat ttatgaagga aggctcccaa taagataaat aagagagttg
    79741 taatccctat tagatggaaa aagtaaacat cataaaccaa gcctataaca aaacaataga
    79801 ctaggtagag atactctgat acttctatcg tctcaaataa gagaaataga aaaagaaaat
    79861 gactagccaa atgtacatgg gggaaaaatg agcccagaag ctggctaata tgggcgtcaa
    79921 ttagaacaaa gaaaggaagc aataaaaata ctccaactcg cttcaactgt ctcattatga
    79981 attccccact aattctatca catctacatt atgagtatct gcactcaatt taacagttac
    80041 ttctcgtgtc aaatagtctg tactatgcgt tgtggcaacc acttcaccaa caggaatatc
    80101 agcaacgtta aagtttccta atccaccagt agtcacctta tctcccgcac taatatcgct
    80161 attactattt aattggctaa ttttaagaac gtcattttcc ttgtcatagc caataataat
    80221 tccataaatt gtagtagagc catgttgaat tttaacagaa atcttatcag cattttccgt
    80281 atttgtcaga aggttgacta tagtagagtt ctcctctact tttgaaacac tcccaatcaa
    80341 gccaccattt gcaatagcta acatgttctc agaagcacct tttgatctac ctgcatctaa
    80401 ggtcaactcc tgcttccaag ataccggaga acgcataata acatctgctg ctaaagtctt
    80461 tgtggcttgc aatttagact tcatatcaag caattggcgc agttgttcat tttctgtctt
    80521 taaactttcc acctcatttg atttaacttc taattggtaa agctgtttct tcaaactttc
    80581 attttcatta tatgttcgtg tcaaatgagc caaatctgat ttgacagaat caaaccactg
    80641 aaaaggtttt tgtacaactc tatcaaccaa tgagattcca tctcctaatt ttgtcacaat
    80701 tgtacttgaa taagttgtcg ctaagagagc tgacacaagc agaacagtga caaaaacaat
    80761 aatgacatat tttgattttt taaaacggtt catatcccta cctttatatc aaaaactgtt
    80821 acagtaactt tttatcaatt cctgaaagct actaagattt taagaaaaat aaacaacaac
    80881 caagtacgag aataacaaga atggtcagcg tatcctttag agtccatttc aattgtcggt
    80941 attgacttct gccttttcca ccctgatagc cacgcgcttc catagcgata gccaaggaat
    81001 ctgcacgttt taaacttgtc gcaaaaagag gaatcaaaat gggaatcatc gactttactt
    81061 tttgaacgat gcttccttct ccaaaatcca ctccacgagc tttctgtgca ttcataatcc
    81121 gcgtcgtatc atccatcaag gttgggacaa aacgcaaact catagacagc atcaatccaa
    81181 tttcatgaac tggaactttc acacgcttta aaggtgctaa taaagcttcg acagctgatg
    81241 ccaaacttaa aggcatggtt gttaaggtta acaaagttga aaagaaaata atcaatacaa
    81301 agcgacaaaa aataatccca gcttgttgca aagcataatc cgtgattctc acaaacgaaa
    81361 actcaaataa aacattccca ttagaaatga aaaatagttg aaaaatagtt gtgaaggcaa
    81421 tcaagaaaaa catagacttc aagccctgaa taaaaaatga aagagatact cctgacaagg
    81481 caataaatat ccctgtcgct ataaaaagaa tcagattcgt caaggggtta ttagcccaaa
    81541 acacaatcaa aatcagtagc atcatagcaa gcaatttgct acgtggatcc aatcggtgaa
    81601 caatcgaatc ccctgggata taacgcccta aaatcatact atccatttag cgactccttg
    81661 aactcctcta tcttaatcgg taatcgttta aatgacacgc ctctatcagc caatcgttta
    81721 caaaaggccg taattttagg tactcccaac tgaacttctt ccataaaaac aacgtcttga
    81781 aagacatcac ttggtttgcc cccctttact aaacgtccct tttccattac atagacttga
    81841 ttcgcatatt cagcaacatc atccatcaaa tgcgttacca agacgatggt catccctgac
    81901 tggtggagtt ttttgaacag ggtcatcaac tcctttctcc ctagaggatc tagaccagct
    81961 gttggctcat ctaagactaa tatagctggc tccatggcaa gtatgcctgc aatggcaaca
    82021 cgtctcattt gtccccctga cagctcaaac ggactacgat caaaaagtga ttcatcaatt
    82081 ccaaccagag ccagtttctc acgcgcagtc ttcacagcat cttcttcaga aactccaaaa
    82141 ttttgcggtc caaaagcaac gtccttcaaa accgtttctt caaaaatctg attttcagca
    82201 aactgaaata ccaagccaac ctgttttcta atttgacgaa tatctttatt tttagaagtc
    82261 gaggtgatta aggtatcaaa aaccctcaca ctcccttgac ttggcaccaa taaaccattt
    82321 aagagttgta aaatagttga tttaccacta cctgtgtgcc caattaaagc tgtataagag
    82381 ccatcttcaa tcgtcaaaga aacatccgac aaagctgctg aagctaaggg agtaccttct
    82441 tgatatgtaa aattcacatt ttctagagca attcccatag cttatcctct agctcacttt
    82501 ctgtcaaata attttcaggt aaattatagc cattatggct caaagatttt tttaattgat
    82561 tagcaaaagg atcgtctaat ccaatttgat ctaaatcatt tcgagagaaa agctcccttg
    82621 gactactagt tgattcaatt tccccttttt tcatgaccaa tacgcgatca ctcatggcga
    82681 cttcttccaa atcatgggta atagaaatga ccgtcatatc atagtctttt cgaattcctt
    82741 ttactgtccc aataagttct ctacgcccct caggatccaa catactcgtt gcttcatcta
    82801 agattaaaat agctggtctt aggactacaa cacctgcaat ggccacacgt tgcttttggc
    82861 cacctgatag acgcgctggc tctctctttt taaagtccaa catgccaact aaatccagag
    82921 cttcttctac tctctttttc atttcttgac gagaaagtcc ctgattttcc aaaccaaaag
    82981 caacatcatc ttcaacagtc gctccaacaa attgattgtc tggattttga aaaaccatac
    83041 cgatttgacg acgtatattc caaacatttt cctcagtcaa acgttggcca tcaattacaa
    83101 tctctccgga ttctgcttcc agtaagccat caattaatcg aaccgtcgtt gatttaccac
    83161 taccattatg ccctacaatc gaaagccatt ctccacgttt cacgtgaaac gtaatatcct
    83221 tcacatcgta gtagttctga ttttctttat agcgaaaaga aagatttttt acatcaatta
    83281 ttgatttcat ttcgaaccaa atgtcccttt aaatacatag gcactaccct tgaaatagtc
    83341 atagccagag tagatagtga aaaataaggc tacataaagt agaacttgac caagcaaagt
    83401 ccaatgtaat agcaagaaaa taatggcaaa catctgacta aaagttttaa tttttccagg
    83461 cattgctgct gctaaaattg ttccaccagt ttcaaccaat aaaagcctta aacctgtcac
    83521 agctaactca cgacagataa tcactgcaac aatccaagcc ggagccatac ctaactcaat
    83581 caacataata aaagccgaca taactagtaa cttatccgcc ataggatctg caaatttacc
    83641 aaaattactg accacattcc atttacgagc taaatatcca tctaaatagt cggtaatact
    83701 ggcaacagca aagataatag ctgcaactat atgactctct atcgaatttc ctatcgttaa
    83761 aataaagata aaaataggta taaagagaat tcgacctatt gttaagagat tgggaatttg
    83821 ttcttttttc attcgttttt ccttaatttt tagtaaaggt tacagtgatt tgtccagtct
    83881 gagctgttaa tttcgataaa tcaacagtct gattatctac tgtcaaatca acacctttta
    83941 caacacctaa cgtaattgtt acaggacttt tagttgcaac tgttgcttct gcacttttct
    84001 tctttggcga taaggttaca ccgccctcaa gttcgctttc tgaaacactg acccaacttg
    84061 taacatctga aactgccaat tgcaatttaa ctgtttcctt acttgtctta taagcgattt
    84121 ctacatgatt tccttcgcct gatacactta tagctgattc tatactagaa gaactactac
    84181 ttgaggagtg gggaacagag ctagttgaac ttgttgattg aaccacactg taattagaaa
    84241 gagaaggctc ctctggttga gtttgaatat agttccaaac ataataagtc acaaaaatta
    84301 aaatcgataa agcaaaaagg ataaaataaa ataaaggtaa aaatgatgtt tttttcttct
    84361 ttttcttact tgaacgtcta cgacctgtca actcatcttc atcaacatct acttcctcat
    84421 aagtaatcat actcccagaa tcataagcat ccaaaacaat ttggtcatct aactcaacag
    84481 cccatgcata ttttttcaag aaagaacgcg tgtaaaaagg acttggaagt tgatcgaaat
    84541 cgtctgcttc cattgcttcc aacatatcta actggatttc tgtctttttc tgcaattcat
    84601 ctaaactcaa tccctgattg attctagcta atcgtaaaac ctctccaatt gttttttttc
    84661 tcatacttgt catcccttct ttctagtgtc tattataata ggttactacg atgggaatat
    84721 tgtaaaatca actatgtcac catcatctat taaatgatgt ccagcatcaa ggacatcctc
    84781 taaagtaatt tcctgtaaaa ttttcggcaa atcaaaaatt gtctcaccat tttcaaaagc
    84841 atcatattgc gttgcaataa attcaagaga gttcatgcta ctgaaaaatt cgccaaacat
    84901 ctctcttttg ataatatcta aatgttcctc tgtaatatct aaatcctttg taaaattacg
    84961 aatagccttc ctaaattgat gagacaaagc aactggctct ttcgtatcca ttgtcaacat
    85021 gacaaaatga aagcgacttg ttacttcaac ttccagagat aaggatgcat caattttacc
    85081 tgattcataa catttttgaa aacgatccga agtccaacca aacatcattg caaacaataa
    85141 ctttaataaa atatgatgtc gatagcaatc cgcttcagaa acttctcgct tacctctaac
    85201 tccaatcgct agtttgggag aagatacttc cattctcata ctatctgttg gctttacagg
    85261 ctgtaaaaac aacttttctc ttgctacttc ctgaaaatct gaatctttca gttctttgct
    85321 ttcaaaatag tcctgtactc gctccacatc aaaattacca actaaaaaca gagacatgtt
    85381 tacaggtttg taaaactttg taaaattttc ttgcaaatta gttagattga tttgggaaat
    85441 ggactcctca cttccaacta tatcagttgc taaaggtgta ccaggataca aattcgctaa
    85501 agttgaaaag aataaacacg aatctggatc atcttggtac atttctcgtt cttgctgaat
    85561 aatatcctgc tctgtcagaa tggaagcttc agtaaagtgt gctgatgtta ccaattcatc
    85621 aagtaaatct aaattttcta aaaaataatc cgttgctgaa aaaagatagt ttgtttttgt
    85681 aaagcttgta aaggcattac tatctgcacc tagactcgta aaagccgaca tcaaatcact
    85741 agaatcttct ctctcaaata atttatgttc aagaaaatga gcaattcctc caggatattg
    85801 ttttacatct ccgtcaactt ctgtgacaaa cgtatctacc gaaccaaact gtacagtgac
    85861 actcccgtaa acctctttaa attccttttt aggcaaaaga gcaactatca atccattggc
    85921 caaacgagtt cgataaacct tttcttttac agctggatag tatttttctt caaaaacaac
    85981 ctttgtcatt ctattccttc cataaagtaa atcgcttgta gtttcacatt attagctact
    86041 ctacaaatag catctttgtc aatctgttca agctttacaa tccaactttt aaagtctgct
    86101 gaagattttc caaataaggc attttgataa gcacgttcaa tcaatgaaga ttgattatct
    86161 taagaaagta acaacgacca acgaatcatt tccttggtct gatttaactc aaactctgta
    86221 aaataacctt tttttaaatc aagcagttga ttattcatca ttttacgagc ctggttacga
    86281 ttttctcgat tgataccagc atacatcctc aagaatccac taaataaatc aagctcactt
    86341 gaaatggtat aagctaatcc agcattttca cggacatttg taaagagctt agaatgagca
    86401 aatccaccaa gtaaaccatt cattacaatc atgggtaaat gttgctcatc accatattta
    86461 gaacggtaat gataacctaa ttccaaaatg gattgtccca catttttccg aaccatacct
    86521 tcctgaagga tattagaata aggttgacaa tactgaacct tcacatctcc ttttcgacct
    86581 ttaaagccaa atgattctaa tacattttga atttcaacct cattaaaatc acctaggaaa
    86641 aagaaatcta ttcgatcatt ggctaaaaat tcttggaaac aagaataaga actttgtgga
    86701 gtttcagcta aaatacgatt tcgtaaatca ctatattcca attgaagacg ttcatcatga
    86761 aaaaacaatt tatccaattc tttatgtgca aaataaaaag aatcatccat atcagctgct
    86821 aaacttgcta gcaattgttt tttctcaatt tcaaataagg ccggatcaaa cccattatca
    86881 actactacgg gtgaaaaaag agtttctttt acaagttcca aaatctgaga ggttagcacg
    86941 tttttcctac ttaaaaactc atcacgaaca taggtaaatg tcaattctat aatgtggctt
    87001 tgccctcttc tgaaacaatt ggttgacata tctgtaccgt atagactggc caagtgtctc
    87061 ctcaaatctt gagaagtggg gtacatctga ttagcagtct ctagcatact tgcactcaac
    87121 atgtgacctg caatcgtatc aagggataat ggagcggtaa aacgcacggt aattttgttt
    87181 gttttaaact tttttgattg gataaaatgt gttgaaattc catgcactaa ctccatgatt
    87241 tacctcctta tatacagact tctattatat cacgaaaacc tgaaattttc ttattccctg
    87301 taacactttt gaaataaaaa tcgcttacat ttctctctgt ctctctcact atttttagga
    87361 aaatatggta taataccaaa gattgaggtg aattatggaa tacaaattat ttgaagaatt
    87421 tattaccctc caagcactac tcaaagaact tggaattaca catagcggag gagctattaa
    87481 atcatttctc tctgaacatt ctgtttactt taatagggaa ttagagagtc gtcgtggaaa
    87541 aaaacttcgt attggcgata aagttgacat ccctgacatg aacattgaca tcttgttgac
    87601 acaacctact tctgaagaac aagatgaata ccaagctgat aaagttgaaa aagaacggat
    87661 cgctaaactt gtcaaaaaga tgaataaggg agttaaaaaa gacaaatcga aacctgcttc
    87721 atcacctaaa agccaacaag ctccacgatt ccctggtaga taatcatgtg gctacaacat
    87781 ctatctctca agacttttcg taattacaaa gaaacgaaaa tagactttaa tcctaaattg
    87841 aatgtctttt tgggacgtaa tgcacaagga aaaacaaaca tgttagaggc tatctatttt
    87901 ttagccttaa cgcgtagtca tcgaactcga acagataaaa atctcattca ttttgatgag
    87961 gaacaacttc atctttcagg tctcgttcag aaaaaaactg gatccattcc cctagaaatc
    88021 gaactaacac aaaaaggccg tgtaacaaaa gttaatcact taaaacaggc acgcctttca
    88081 gattatgtag gacacatgaa tgttgtctta tttgctcctg aagatttaca actaattaaa
    88141 ggagcacctt cgattcgacg aaaattcatt gatatggaac ttgggcaaat taagccaatc
    88201 tacttatctg acttaaccaa ttataaccac atcctaaagc aaagaaacac ttacctaaaa
    88261 tcagatcaaa aaatagatga aacattcctt tctgtgttag atgatcagct agttgattat
    88321 ggatgtcgtg taatgaatca ccgcttagat ttcataaaaa aactagaatc atttgggcgt
    88381 aagaaacatt ttgaactctc taatcagatc gaagagttgt caatatccta tcaatcttct
    88441 gtcaatataa ctgacaaaca aaacttatcc gaatctttca aaattgcttt agaaaaaagt
    88501 aaatccagag atttatttaa aaagaatact ggtgtcggtc ctcatcgaga tgacatttct
    88561 ttttatataa atgggatgga tgctagtttc ggaagccaag gtcaacatcg tagtctcgtc
    88621 ctctcgataa aattagcaga aatcgaatta atggaaagca ttaccacaga atctccgata
    88681 ttactgcttg acgatgtgat gagtgaactt gacaacacta gacagttaaa attattagaa
    88741 acgatttctc aatcaatcca aacctttatc acaacaacaa gcttagacca tcttcaaaat
    88801 ctgccagaaa atctaagtat cttcactatt caggatggta aagctgctgt aaatggaaat
    88861 tgacaacatt ataaaagaac tcctgttttc actggagttc ttttcattgt tttttacata
    88921 gaataatttg gtgcctcatt agtaatttgc acatcatgag gatggctttc tttcaaacca
    88981 gcaccagaca tttcaataaa ttgagcatta tcgtgtagtt ctttaaggtt agctgcacca
    89041 cagtaaccca taccagagcg aataccacca atcatttgga aaacaatatc agctgccgct
    89101 cctttataag caacacgacc ttcaattcct tctggaacaa gcttgtttgc ttcattgaca
    89161 gaaccttgga aataacggtc gcttgaacct ttcttcatag cagcaattga tcccatacca
    89221 cggtaagtct tgaatttacg tccttggaag atttcagttt cgcctggagc ttcatcagtt
    89281 ccagcaaaca tagatccaag cataacagca tttccacctg cagcaagtgc ttttacaata
    89341 tctccagaat acttgatccc accgtcagca ataatcgttt taccatattc gcgcgcaaca
    89401 gctgcagcat cgtagatagc tgttacttgc ggaacaccaa caccagcaat cacacgagta
    89461 gtacagatag aacctggtcc aataccaacc ttaacaacgt ctacacccgc ttcataaagg
    89521 gcacgtgcac cttcagcagt agcaatattt ccagcaatca aagtccgatc tgggaaatga
    89581 gcacgaatct cggcaatttt acgcaagaca cctgcagaat gaccatgtgc agtatcaata
    89641 acaatcgcat ccgctcctgc ctcaaaaaga gcctctgcac gttcaaatgt atctgaagta
    89701 acacctactg cacctgcaac tagaagacga ccaaactcat ctttagccgc atttggaaac
    89761 tcaataactt tttcaatatc tttgatagtg atcaaaccag aaagactgcc ttcttcatcg
    89821 accaacggaa gtttttcaat acgatgctct tgaagaatac tctcagccgt tgcaagatcc
    89881 gtacccacag gagcagtaac aagattttca ctagtcatat ggtttgaaat tggttgatta
    89941 taatctgaaa taaaacgaag atctcggttt gtcaaaatac caaccaattt acgattttca
    90001 agtgtttcaa caactggaac accactgatg cggtaacgac ccataagctc atctgcttca
    90061 gcaattgtat gttcaggcgt caagaagaac ggatcaataa taactccatt ttcagaacgt
    90121 tttaccttac gaacctcgtc tgcttgttga gcaattgaca tgtttttatg gataactccg
    90181 agaccacctg cacgagcaat agcaatggcc atttgactct ctgtaactgt gtccatggca
    90241 gcggtaataa ttgggatatt taaagtcaaa ttatctgcca atttagttgt taaatctgca
    90301 tcgttaggca acacatgact ttcagctgga ataagcaata catcatcaaa ggtaaaacct
    90361 tttttcaaaa atttagtgtc ccaattagac attcgaagtt tcctcttttc tttctttttg
    90421 agcttgactc tttttatatt gtatctatca taccattcta tacaaatttg tcaaatgtta
    90481 caggggtaaa taaaaaacta tcttttcttc gagatagaaa tgatagtttc ttgtaaaata
    90541 aacttagtta aagtaatgaa gtcccattgc cccttttacc tcagataggg tttgacccgc
    90601 aacttcacgc gctctttcac tacctttttg aatcatatta taaacttctc ccatatcctt
    90661 agcaaattca atacggcgct cacgaatagg acccagttca cgttctaata tttcaagtag
    90721 ataacgcttg gtcttcacat caccaagacc acctcgttga taacgttctt tcatatcagc
    90781 aatttcttga gcatcttctg gacgaccaaa aacatctaga taatggaaaa ccatatttcc
    90841 ctcaatctta cctggatcct caacgcggat atgatctgga tctgtataca tactcattac
    90901 ttttttacgc aaagtatccg catcatcagc taaataaata ccattattta gtgatttaga
    90961 cattttagca tttccatcta aaccaggcaa acgccctgct ctctcatttt ctggataaat
    91021 accttccggc tctaccaaga catcacagtt atatgcattg ttaaaagaac gaacaatttc
    91081 acgagtttgc tcaatcattg gtttctgatc tgtcccaaca ggaacataat tagccttgaa
    91141 agctgtgata tcagctgctt gagcgattgg atagaccaag aatcctgtcg gaatgctttc
    91201 tccaaatcct ttctgagaaa tctctgtctt gactgttgga tttcgctcca aacgtgctaa
    91261 cgaaactaga ttcatataat acatagacaa ctcagccaac tctggaatct ggctttgaat
    91321 aaaaatagtt gacttatttg gatccaatcc aactgcaaga taatccaaag ccacatttcc
    91381 gatagactct acaatggttt gaggatcttt ggcatgatct gtcaaggctt gttggtcagc
    91441 caagaacaca aacatatcat acttatcctc ttcctgtaat aatactcgat ttttgagact
    91501 tccaacataa tgtccaatat gcaattttcc tgttggacgg tctcctgtta aaataatggg
    91561 tttagtcatt ttgttctcct tcgatatagt cctttcaatt atagcatttt tttaatgaaa
    91621 taaccgtaat ttatcaaaat aaatcagcct tgctttttac agatttgtgt aaaggatact
    91681 gtaaatataa tttacacaaa attgacaaat aaaaatttga atatttctgt attttctagt
    91741 agaataaata tattacacat ataggagaaa aatattgctt acagtatctg atgtttcact
    91801 acgttttagt gatcgcaaac tttttgatga tgtcaatatc aaatttacag aaggaaatac
    91861 ttacggatta atcggtgcta atggtgccgg aaaatcaacc tttttaaaaa ttttagctgg
    91921 agatatcgaa cctactactg gtcacatctc tcttggtcca gatgaacgtc tctctgttct
    91981 tcgtcaaaat cactttgact acgaagatga acgtgccatt gatgtcgtta tcatgggaaa
    92041 tgaaaaactt tatagcatca tgaaagagaa agatgctatc tacatgaagg aagatttctc
    92101 agacgaggac ggggttcgtg ctgccgaact cgaaggagag tttgccgaac ttggaggttg
    92161 ggaagcagag agtgaagcct ctcaactact tcaaaaccta aacattccag aagaattaca
    92221 ctaccaaaac atgagcgaat tggccaacgg tgaaaaagta aaggttctcc tcgccaaagc
    92281 actttttggt aaaccagatg ttcttctctt ggacgagcct actaacggtt tggatatcca
    92341 atcgattact tggttagaag acttcttaat tgactttgat aacacagtta tcgtagtatc
    92401 ccacgaccgt cacttcttaa acaaagtttg tactcacatg gccgaccttg actttggaaa
    92461 aatcaaactc tatgtcggaa actacgactt ctggaaggaa tcttctgagc ttgctgctaa
    92521 attgctagca gaccgtaatg ctaaagcaga agaaaaaatt aaacaattgc aagaattcgt
    92581 tgctcgtttc tctgccaatg cttctaagtc aaggcaggca acatcacgta agaaaatgct
    92641 tgataagatt gagctagaag agattgtgcc atctagtcgt aaatatccat ttatcaactt
    92701 taaagcggaa cgtgagattg gtaatgatct cttgacagta gaaaatctaa ctgtaaagat
    92761 tgatggtgaa actatcttgg ataatattag tttcatcttg cgtccagatg ataagacagc
    92821 acttattgga caaaatgata ttcaaacgac tgcattaatt cgtgcaatca tgggagatat
    92881 tgactatgaa ggaactgtca agtggggagt tacaactagt caatcttacc taccaaaaga
    92941 taactcagct gattttgcag gaggagaatc aattcttgac tggttgcgtc aattcgcaag
    93001 taaagaagaa gatgacaata ctttcctacg tggcttcctc ggccgtatgc tcttctctgg
    93061 agatgaagtt aacaaacctg taaatgtctt gtcaggggga gaaaaagttc gtgtcatgct
    93121 ttcaaaactc atgctcttaa aatcaaatgt ccttgtactt gatgatccaa caaatcactt
    93181 ggacttggaa tctatctcaa gcttgaatga tggattgaaa aactttaaag aatcaatcat
    93241 ctttgctagc catgaccacg aatttattca aactttggct aaccatatca ttgtcttatc
    93301 taaaaatggc gtcattgacc gtatcgatga aacctacgat gaattcctag aaaatgcaga
    93361 agtacaagca aaagttaaag aactttggaa agactaaata aaacttcaaa actcagttgg
    93421 gttaaccaac tgagttttct atcattctac gaggtaacat gaaatcattt ttaaaaacat
    93481 atcgaaccta ttttatttct ttcatcattc ctgtagtgat tatgtctgga gtatatctat
    93541 ctcaaggtat ctactggaat agcgacaact ctcctctatt aggagatggc tttcatcaat
    93601 acgttatttt tgatgtagcc ttacgaaata tcctacatgg aaatagtagt ctgttttaca
    93661 cctttacaag tggtctaggg ctaaacttct atgccctatc tagttattac ttgggtagtt
    93721 ttctcgcgcc tctagtttac ttttttgatc taacgaatat gccagatgct gtctatctga
    93781 ccactctctt aaaatttgga ttgattggtc tgtcaacctt ttttaatttg aataaattgt
    93841 ttcaatctat ccctcagact ttaaaactag ccttatctac ttcctatgct ctgatgagtt
    93901 tcactgtcag tcaattagag ataaaaacct ggctagatgt ttttatcttg attcctttaa
    93961 ttataactgg tttacatcta ctgataactg aaaagaaact cctattgtac tttacaagtc
    94021 tgtcaatctt atttattcaa aattattatt ttggatatat gacagtattg tttcttattt
    94081 tctggtatct ctgtcaaatt tcgtgggact ttaagactcg aaaatcatct gttcttgatt
    94141 tcatagttat ctccttttta gctggtatgg ctagtttgat tatgactctt cccactctat
    94201 ttgatttaca gacacatggg gaaaaattga ctgaagttac aaagtttcaa actgaaagta
    94261 gctggtatct tgatctcttt gctaagcaat tcattggttc ctttgacaca acaaagtatg
    94321 gggccatccc aatgattttt gttggactat ttccctttat tttgaccatt ttatttttta
    94381 cgctgaaatc tattaagttt cacgtgaaac tcatatatgt aatattcttt gcatttctaa
    94441 ttgcaagctt ttacatagaa gctcttgact tattttggca aggcatgcat actccaaaca
    94501 tgtttttaca tcgctatgct tggattttct ctaccttgtt aatttacaca gcagcagaag
    94561 tcttaaagcg tctgaaagaa cttaaagtct ggaatttttt agtttcgctt tttcttgtag
    94621 tagcaggatt tttagctacc atctatctaa aatcgcatta ttctttttta acagatttga
    94681 atattctgct tactcttgaa tttttggttg tctattctct tttactcctt gcagttatca
    94741 aaaagtttat atctgtgaat ctatttgcca ttctaatctc tttatttata ctggttgaaa
    94801 tgagtttaaa tgcttcatct caaatggacg gaattgctaa ggaatgggga tttgcttctc
    94861 gaagtgctta tagtcgagat atcccagcta tggaatcttt ctcaacatat attggaaatc
    94921 aatttactcg tactgaaaaa ctacaaactc agacaggaaa tgacagtatg aaattcaact
    94981 acaatggaat ctctcaattt tcatctgttc gaaatcgttc atcaagctct actttagata
    95041 aacttggttt taaatcctct gggactaatc tcaatctccg atatgcaaat aatagtattt
    95101 tggctgatag tttatttggt atccagtaca atatctcaga cagtcctatt gataagtatg
    95161 gctttaaaga tatctatcaa aaagataatc ttaccctata tgaaaatcaa tactctcttc
    95221 cgattgcagt tgctagtcaa tctgtttaca atgatgtcaa gttcaatgaa cataccttgg
    95281 ataatcaggc ctcattttta aatcaacttg ctaacgtcaa ttttgattat ttttctccaa
    95341 taccttatga aaaaacagaa aaaatagaaa atactaatga tttgattagt gtcacaagtt
    95401 cttcaaatga agatgcagca atccagtatc aaattgaagt tccagaaaac agccaagttt
    95461 atctctcttt cataaacctt cacttttcta acgataaaca aaagaaggtt gacatacttg
    95521 taaatggtga aaaaaagact tttacaactg ataatgtctt ctccttcttt aatctaggat
    95581 atactaaaga gaaaaaaact ttcaatatca atgttagttt ccctggaaat tcacaagtat
    95641 catttgaatc tcctaccttc tatcgtttag ataccaaaac tttcaccgag gcaattcaaa
    95701 aaattaaaga acaacctgtc acagtatcaa cttctaaaaa caaggttttt gctacatatg
    95761 atgtccaaca agatacatct attttcttca ccattcctta tgacaaaggt tggtctgcct
    95821 accaagatgg taagaaaata gaaattaaac aagctcaaac tggatttatg aaagttgaca
    95881 ttcccaaggg gaaaggaact attacacttt ccttcattcc caatggtttt attactggag
    95941 caatctgttc ctttacttct ctcttactat ttggaatcta taatcacaga cgaaagtcat
    96001 ctaaggcata aaaaatcgac cagttaatcg gtcgattttt ttaatcttct tccattttct
    96061 tatgttgata ggctagcata cctaaaccag taaatagagc tagtatgaca gctaggaaaa
    96121 tgacttgttg atagagattg tttgtcgtaa tcccgaaact gaatagctca ttggtaacca
    96181 gggattaata gatctaaaga aatcatttgt caaagcaagt ggataagtac ctgcacttga
    96241 tgctaactgt agtaaaagca aaataagtga gaaaaaagct cctatacggc tattccacgt
    96301 tgctaaagtg gtcaccatag acatgaatac taaacttgtt aggatgatga gaataaatat
    96361 tctcatctca tgattagcag ttaaaccaat aagctgaact cctccatata ccaaaattcc
    96421 tgccaaaaca gctataatac catttatttc agctcaagat ttcaaccaag cccaacggct
    96481 ctctggatga cgtcctgaag gcaatttcgc aaatatcata tttgttgata ttgctgcaaa
    96541 aaaaagagca actgatatca tataaggagc tattgcgatt ccatttacag gaacttgatc
    96601 attgtctgtt tttgaaagat tgagtggatt tgacaaaatc tctgcatttt tagattctgt
    96661 tgatactgat ttgagttgat cactagcatt acctagtcct tgtcctaaag aagcaagtcc
    96721 tgtctgtaaa tcttccaatc cagaagttaa ctttgttcca ccttctgcta gtttcccaga
    96781 tccatctgcc aatttattag ctctattctc taatggagaa gcacctgaaa gtaactgact
    96841 ggatttgtct gctaattgcc cagatcctga ttgtaattaa ccaactcctg ctgtcaatct
    96901 agaagatttt tgtgtcaagg tgtttgagcc tgaaactagt ttatccaaac tacctgtcaa
    96961 ggtggcattt ttttcactta gttgacttgc gccctgagaa actttatcaa cacctgtagt
    97021 atatgcgttt acacctgatg caatcaactg actggcagga actaatttgc tagtaacagc
    97081 tccttgtatc tctgttaatc cacttgacaa tcctatcaaa gaagtagaag taataggtaa
    97141 tacttgatta gcttgatttt ttaaagtcga aagattagaa gattgatttt gtaagttttc
    97201 taaacttccc tgtaaacctt gtactaaagc tacaattgac tgagccgatt gaatactatc
    97261 agttgaattt tgagatacag aatcacttat cccagtttgt tgctcacttg tcaatgattg
    97321 ataagctgct gtcgattgaa tattggataa tgtagttgtt tttttagata aatcacttgg
    97381 taaagtcact accatatagt aatcgccatc ttccaatccc ttctttcctt cctcttcatc
    97441 tacaaaatga aaatccaagg ttttattttc ttttaaattg gacaccatgt cttttcctat
    97501 tgccatagta ttaccattat aggaagcctc tttatcatta tttacaactg ccacaggtaa
    97561 gtcagacaat tgcccatatg gatcccacat tgatgacaaa aatatgatat tgtacagagc
    97621 tggaataaga gaaatcccga tcatgacaat aataaaggtt gtttttttta aattgctttc
    97681 cattctttaa acatag
//
LOCUS       DBERCA010000003        78434 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.003, whole genome shotgun sequence.
ACCESSION   DBERCA010000003 DBERCA010000000
VERSION     DBERCA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 78434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.003"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..604
                     /locus_tag="ACL719_000201"
                     /pseudo
     CDS             1..604
                     /locus_tag="ACL719_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            601..819
                     /locus_tag="ACL719_000202"
     CDS             601..819
                     /locus_tag="ACL719_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698244.1"
                     /translation="MSISPRFEALEQAIAFKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(876..1118)
                     /locus_tag="ACL719_000203"
     CDS             complement(876..1118)
                     /locus_tag="ACL719_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGS0698245.1"
                     /translation="MKKDIHPEYRSVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1214..2149)
                     /locus_tag="ACL719_000204"
     CDS             complement(1214..2149)
                     /locus_tag="ACL719_000204"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGS0698246.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2253..2696
                     /locus_tag="ACL719_000205"
     CDS             2253..2696
                     /locus_tag="ACL719_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGS0698247.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2812..3078
                     /locus_tag="ACL719_000206"
     CDS             2812..3078
                     /locus_tag="ACL719_000206"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGS0698248.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            3075..3449
                     /gene="crcB"
                     /locus_tag="ACL719_000207"
     CDS             3075..3449
                     /gene="crcB"
                     /locus_tag="ACL719_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS0698249.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            3443..3772
                     /gene="crcB"
                     /locus_tag="ACL719_000208"
     CDS             3443..3772
                     /gene="crcB"
                     /locus_tag="ACL719_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS0698250.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3891..4238
                     /gene="rplS"
                     /locus_tag="ACL719_000209"
     CDS             3891..4238
                     /gene="rplS"
                     /locus_tag="ACL719_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGS0698251.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4280..4351
                     /locus_tag="ACL719_000210"
     tRNA            4280..4351
                     /locus_tag="ACL719_000210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4313..4315,aa:Arg,seq:cct)
     gene            complement(4406..5212)
                     /gene="yidA"
                     /locus_tag="ACL719_000211"
     CDS             complement(4406..5212)
                     /gene="yidA"
                     /locus_tag="ACL719_000211"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGS0698252.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(5225..6529)
                     /locus_tag="ACL719_000212"
     CDS             complement(5225..6529)
                     /locus_tag="ACL719_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS0698253.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            6601..6978
                     /locus_tag="ACL719_000213"
     CDS             6601..6978
                     /locus_tag="ACL719_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGS0698254.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            7069..7401
                     /locus_tag="ACL719_000214"
     CDS             7069..7401
                     /locus_tag="ACL719_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGS0698255.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            7413..8984
                     /gene="ffh"
                     /locus_tag="ACL719_000215"
     CDS             7413..8984
                     /gene="ffh"
                     /locus_tag="ACL719_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGS0698256.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKMDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(9179..10462)
                     /locus_tag="ACL719_000216"
     CDS             complement(9179..10462)
                     /locus_tag="ACL719_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGS0698257.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKKRVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(10716..11429)
                     /gene="rsmG"
                     /locus_tag="ACL719_000217"
     CDS             complement(10716..11429)
                     /gene="rsmG"
                     /locus_tag="ACL719_000217"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGS0698258.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            11523..12083
                     /locus_tag="ACL719_000218"
     CDS             11523..12083
                     /locus_tag="ACL719_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGS0698259.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            12085..12984
                     /gene="htpX"
                     /locus_tag="ACL719_000219"
     CDS             12085..12984
                     /gene="htpX"
                     /locus_tag="ACL719_000219"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGS0698260.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(13133..14692)
                     /locus_tag="ACL719_000220"
     CDS             complement(13133..14692)
                     /locus_tag="ACL719_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698261.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQSVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(14931..15247)
                     /locus_tag="ACL719_000221"
                     /pseudo
     CDS             complement(14931..15247)
                     /locus_tag="ACL719_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
     gene            15417..15959
                     /locus_tag="ACL719_000222"
     CDS             15417..15959
                     /locus_tag="ACL719_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGS0698262.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            15959..16588
                     /gene="nth"
                     /locus_tag="ACL719_000223"
     CDS             15959..16588
                     /gene="nth"
                     /locus_tag="ACL719_000223"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGS0698263.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKLSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            16799..17320
                     /gene="pyrR"
                     /locus_tag="ACL719_000224"
     CDS             16799..17320
                     /gene="pyrR"
                     /locus_tag="ACL719_000224"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGS0698264.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            17339..18262
                     /locus_tag="ACL719_000225"
     CDS             17339..18262
                     /locus_tag="ACL719_000225"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGS0698265.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            18312..19391
                     /locus_tag="ACL719_000226"
     CDS             18312..19391
                     /locus_tag="ACL719_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGS0698266.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            19704..22880
                     /gene="carB"
                     /locus_tag="ACL719_000227"
     CDS             19704..22880
                     /gene="carB"
                     /locus_tag="ACL719_000227"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGS0698267.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLSSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(23156..23965)
                     /locus_tag="ACL719_000228"
     CDS             complement(23156..23965)
                     /locus_tag="ACL719_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0698268.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(23967..24770)
                     /locus_tag="ACL719_000229"
     CDS             complement(23967..24770)
                     /locus_tag="ACL719_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0698269.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(24780..26267)
                     /gene="tacF"
                     /locus_tag="ACL719_000230"
     CDS             complement(24780..26267)
                     /gene="tacF"
                     /locus_tag="ACL719_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGS0698270.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            26603..27310
                     /locus_tag="ACL719_000231"
     CDS             26603..27310
                     /locus_tag="ACL719_000231"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS0698271.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            27315..28337
                     /locus_tag="ACL719_000232"
     CDS             27315..28337
                     /locus_tag="ACL719_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGS0698272.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            28354..29223
                     /gene="licA"
                     /locus_tag="ACL719_000233"
     CDS             28354..29223
                     /gene="licA"
                     /locus_tag="ACL719_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGS0698273.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            29213..30091
                     /locus_tag="ACL719_000234"
     CDS             29213..30091
                     /locus_tag="ACL719_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0698274.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            30103..30792
                     /locus_tag="ACL719_000235"
     CDS             30103..30792
                     /locus_tag="ACL719_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGS0698275.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            30878..31726
                     /gene="dprA"
                     /locus_tag="ACL719_000236"
     CDS             30878..31726
                     /gene="dprA"
                     /locus_tag="ACL719_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGS0698276.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            32616..33659
                     /locus_tag="ACL719_000237"
     CDS             32616..33659
                     /locus_tag="ACL719_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGS0698277.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            33826..35913
                     /gene="topA"
                     /locus_tag="ACL719_000238"
     CDS             33826..35913
                     /gene="topA"
                     /locus_tag="ACL719_000238"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGS0698278.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            36134..36493
                     /locus_tag="ACL719_000239"
     CDS             36134..36493
                     /locus_tag="ACL719_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGS0698279.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            36586..37218
                     /locus_tag="ACL719_000240"
     CDS             36586..37218
                     /locus_tag="ACL719_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGS0698280.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            37262..37918
                     /locus_tag="ACL719_000241"
     CDS             37262..37918
                     /locus_tag="ACL719_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGS0698281.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            37988..39031
                     /locus_tag="ACL719_000242"
                     /pseudo
     CDS             37988..39031
                     /locus_tag="ACL719_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            39043..40080
                     /gene="leuB"
                     /locus_tag="ACL719_000243"
                     /pseudo
     CDS             39043..40080
                     /gene="leuB"
                     /locus_tag="ACL719_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            40077..40346
                     /locus_tag="ACL719_000244"
     CDS             40077..40346
                     /locus_tag="ACL719_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGS0698282.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            40350..40709
                     /gene="leuD"
                     /locus_tag="ACL719_000245"
     CDS             40350..40709
                     /gene="leuD"
                     /locus_tag="ACL719_000245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGS0698283.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            40871..41493
                     /locus_tag="ACL719_000246"
                     /pseudo
     CDS             40871..41493
                     /locus_tag="ACL719_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            41657..43588
                     /locus_tag="ACL719_000247"
     CDS             41657..43588
                     /locus_tag="ACL719_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGS0698284.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYISPEEVETDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            43575..44891
                     /locus_tag="ACL719_000248"
     CDS             43575..44891
                     /locus_tag="ACL719_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGS0698285.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IVVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            45066..46052
                     /locus_tag="ACL719_000249"
     CDS             45066..46052
                     /locus_tag="ACL719_000249"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGS0698286.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            46116..46187
                     /locus_tag="ACL719_000250"
     tRNA            46116..46187
                     /locus_tag="ACL719_000250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46149..46151,aa:Arg,seq:ccg)
     gene            46370..47068
                     /gene="rnc"
                     /locus_tag="ACL719_000251"
     CDS             46370..47068
                     /gene="rnc"
                     /locus_tag="ACL719_000251"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGS0698287.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            47059..50598
                     /gene="smc"
                     /locus_tag="ACL719_000252"
     CDS             47059..50598
                     /gene="smc"
                     /locus_tag="ACL719_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGS0698288.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVYNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            50595..51389
                     /locus_tag="ACL719_000253"
     CDS             50595..51389
                     /locus_tag="ACL719_000253"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0698289.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            51389..52207
                     /locus_tag="ACL719_000254"
     CDS             51389..52207
                     /locus_tag="ACL719_000254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0698290.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            52211..53488
                     /gene="ftsY"
                     /locus_tag="ACL719_000255"
     CDS             52211..53488
                     /gene="ftsY"
                     /locus_tag="ACL719_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGS0698291.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(53534..55021)
                     /gene="zwf"
                     /locus_tag="ACL719_000256"
     CDS             complement(53534..55021)
                     /gene="zwf"
                     /locus_tag="ACL719_000256"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGS0698292.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(55170..55910)
                     /locus_tag="ACL719_000257"
     CDS             complement(55170..55910)
                     /locus_tag="ACL719_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0698293.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(55910..58075)
                     /locus_tag="ACL719_000258"
     CDS             complement(55910..58075)
                     /locus_tag="ACL719_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS0698294.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            58245..59198
                     /locus_tag="ACL719_000259"
     CDS             58245..59198
                     /locus_tag="ACL719_000259"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGS0698295.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAIVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            59275..61263
                     /gene="uvrB"
                     /locus_tag="ACL719_000260"
     CDS             59275..61263
                     /gene="uvrB"
                     /locus_tag="ACL719_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGS0698296.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            61273..61836
                     /locus_tag="ACL719_000261"
     CDS             61273..61836
                     /locus_tag="ACL719_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS0698297.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            61802..62290
                     /locus_tag="ACL719_000262"
     CDS             61802..62290
                     /locus_tag="ACL719_000262"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698298.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            62363..62830
                     /locus_tag="ACL719_000263"
     CDS             62363..62830
                     /locus_tag="ACL719_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS0698299.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(62827..63342)
                     /locus_tag="ACL719_000264"
     CDS             complement(62827..63342)
                     /locus_tag="ACL719_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGS0698300.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(63354..63881)
                     /locus_tag="ACL719_000265"
     CDS             complement(63354..63881)
                     /locus_tag="ACL719_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0698301.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(64163..64726)
                     /locus_tag="ACL719_000266"
     CDS             complement(64163..64726)
                     /locus_tag="ACL719_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0698302.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGITLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(64710..65261)
                     /gene="coaC"
                     /locus_tag="ACL719_000267"
     CDS             complement(64710..65261)
                     /gene="coaC"
                     /locus_tag="ACL719_000267"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGS0698303.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(65273..65962)
                     /gene="coaB"
                     /locus_tag="ACL719_000268"
     CDS             complement(65273..65962)
                     /gene="coaB"
                     /locus_tag="ACL719_000268"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGS0698304.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            66192..67862
                     /locus_tag="ACL719_000269"
     CDS             66192..67862
                     /locus_tag="ACL719_000269"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGS0698305.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVDAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWGKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            68190..69365
                     /gene="mutY"
                     /locus_tag="ACL719_000270"
     CDS             68190..69365
                     /gene="mutY"
                     /locus_tag="ACL719_000270"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGS0698306.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYVQANLDSSQD"
     gene            69420..70124
                     /gene="yycF"
                     /locus_tag="ACL719_000271"
     CDS             69420..70124
                     /gene="yycF"
                     /locus_tag="ACL719_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGS0698307.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            70117..71466
                     /gene="vicK"
                     /locus_tag="ACL719_000272"
     CDS             70117..71466
                     /gene="vicK"
                     /locus_tag="ACL719_000272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGS0698308.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            71468..72277
                     /locus_tag="ACL719_000273"
     CDS             71468..72277
                     /locus_tag="ACL719_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS0698309.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            72368..72610
                     /gene="relB"
                     /locus_tag="ACL719_000274"
     CDS             72368..72610
                     /gene="relB"
                     /locus_tag="ACL719_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS0698310.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            72612..72866
                     /locus_tag="ACL719_000275"
     CDS             72612..72866
                     /locus_tag="ACL719_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS0698311.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            72913..73656
                     /locus_tag="ACL719_000276"
                     /pseudo
     CDS             72913..73656
                     /locus_tag="ACL719_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(73957..74943)
                     /locus_tag="ACL719_000277"
     CDS             complement(73957..74943)
                     /locus_tag="ACL719_000277"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGS0698312.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            75137..77605
                     /gene="gyrA"
                     /locus_tag="ACL719_000278"
     CDS             75137..77605
                     /gene="gyrA"
                     /locus_tag="ACL719_000278"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGS0698313.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            77605..78348
                     /gene="srtA"
                     /locus_tag="ACL719_000279"
     CDS             77605..78348
                     /gene="srtA"
                     /locus_tag="ACL719_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGS0698314.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT        23860 a        14037 c        17279 g        23258 t
ORIGIN      
        1 gctcaaacca ttgtcgcatc taaaaataaa atctcaattc aagaagacaa gaaagcgctt
       61 taaaaccagt agacgttttc ataaggttcg cttgatgtac caagatgaag ctggtttcgg
      121 tagaatcagt aaactgggat cttgttggtc tccaatagga gtaggtccac atgtccatag
      181 tcactatata cgagaatttc gctattgtta tggagctgtt gatgcccata caggcgaatc
      241 atttttctta atagctggta gatgtaatac tgagtggatg aacgcctttt tagaagagct
      301 ttcacaagct tatccagatg attatttttt actcgttatg gacaatgcta tatggcataa
      361 atcaagtatc ttaaagattc cgactaatat tggttttgca tttattcctc catacacacc
      421 agagatgaac cccattgaac aagtgtggaa agagattcgt aaacgtggat ttaagaataa
      481 ggcctttcga actttggaag atgtcatgaa tcaacttcaa gatgtcatac aaggactgga
      541 gaaggaggtg ataaagtcca tcgttaatcg gagatggact agaatgcttt ttgaaagcag
      601 atgagtataa gtcctcgttt tgaagccttg gaacaggcta ttgccttcaa agatttggaa
      661 aaagtccgag aagcttttaa gaaaatgaat tccacttgga ccatcaatga aagtgtggtt
      721 cgttacaata gtatagctca ttatggacgt gttgaaacag ctatttcctt cctgcctagt
      781 agcatggaaa ccgaacctac agatgagtct ggaacatgaa aacagcagtt gatttcaaga
      841 aacaaaaaag aacagccgaa actgttctct tatcattatt tgagaccgta ttttttgttg
      901 aaacgatcca cgcgtccatc tgcttgagtg aacttttgac gtccagtgta gaatgggtgt
      961 gagtctgatg aaatttccac acggatcaat gggtaagttt cgccttcgaa ctcaactgtt
     1021 tcgttagagc gttttgttga accgctaagg aattggtaac cagtagttgt gtccatgaag
     1081 acaactgagc gatattctgg atggatatct tttttcattt tgcaatattt cctttctgcc
     1141 atggtctctt tgcgagccat agattgttac catactagtc tatcagattc tgaaaagttt
     1201 ggcaagtatt ttatcagttt ttaagcaagt tttttaactt ttggtagatg atttcgtttt
     1261 cttctaggct ataggaatta gcaccacttg ctagagggtg gcctccacca tcatgctcct
     1321 tggcgatttc attgatagga tggattttac tgcgtaagcg aacgcggtag tggccatcag
     1381 cctgttcgac aaaaattccc cagagactca ctctgtcaat gcgtccaggt gcaccgacaa
     1441 tggctgcagt ttcagcatcg gttacattgt attgtttcaa gacttcttga ctcaggataa
     1501 cgcgagcagc accattttca tccacttcca gatggtcgta gatgtagcct tgcagtttag
     1561 caattttgta gctcatagtg tccattttgc gagtgagagc cgcaaagtca aagttatgtt
     1621 ctctcaaata agcagccagg cgaagagtcc gtgcagtggt agaagggtag aggaagcgac
     1681 ctgtatcacc gacaattcct gcaaagagca actcagcatc gcgatctgcc aaggctagtt
     1741 gggttgtttg ggcaaatagg gtgatcatct cgctagcgct acttgaacta gtatcgaccc
     1801 aagacaggtc accgtataca tcatcatttg gatggtggtc aatcttaatg agaaaatcac
     1861 cttgactata gcgcttatca tcgatacgag cagtattagc tgtatcacag acgatgacaa
     1921 gtgcgccttg gtaggctcta tcttcaacaa gatccatctc agccatccaa gtaagagttg
     1981 gttcatcaaa accgacggct ttgatggttt tttctgggaa atgatgttcc agcaaggctt
     2041 tcaatcccac ctgacttcct aaggcatcag ggtctggttt catatgacga tgaatgataa
     2101 tggtgtcgta ttctttaatt ttttctaaaa tttgttggca aatctccata aataacctca
     2161 attctttctc atttcattgt agcacaagaa tttttatttt taaaatgaaa aagatgtgat
     2221 ataatggaga aagataaatt gaggaggacg atatggcatt agcaaaaatt gtatttgcca
     2281 gtatgaccgg taataccgaa gaaattgcag atattgtagc agacaaatta cgtgacttgg
     2341 gcttggatgt tgatgttgat gaatgtacaa ctgttgacgc ttcagacttc ttggaagcag
     2401 acatcgctat cgttgcgacc tatacttatg gtgatggtga attgccagat gagatgatgg
     2461 acttctacga agacctagca gatctcaact tgaatggcaa aatctacgga gtggtcggct
     2521 caggagatac cttctacgat gaattctgta aggctgttga tgactttgac cgtgtctttg
     2581 tgtcaacagg agcagaaaaa ggttcagagt gtgttaaagt tgatctttct gccgaggaag
     2641 aagatattga acgcttggaa caattcgcag aagaattggt tgctaaagta ggataagcat
     2701 aaaagtgcgc tgatgtaatc aatcagtgca tttttcttat cgagagttgg gattttacta
     2761 gcctaattga tggctttttg atacaataat tcaaataaag gaggagactg tgtggattta
     2821 gatattattc gtcaagaaat cgatcaaatc gacgaccaaa ttgtcaaact gttagaagaa
     2881 cgaatgcact tagttgaagg agttgtcgct tataagaagg cttcagggaa accgatttta
     2941 gatactaaga gagaagcagt tatttttgaa aaggttagaa gtcgtgtaga ggacaagcgc
     3001 tatcaggaga ctattgtcgc gactttttcg gacatactca aacgttcgcg tgattatcag
     3061 gatcaaaata tcaaatgaaa aaagaacaat tttatccgct agggattttt ctagctgcta
     3121 tgttgggcgg acttgtccgc tacctagttt ccacttggtt accagccagt ccagatttcc
     3181 cttggggcac tctctttgtc aactatctgg gaattttctg cttgatttat cttgtcaagg
     3241 gctatctggt ctataagggg actagtaagg gcttgatttt agcactgggg acgggttttt
     3301 gcggaggttt aacaactttt tccagcctaa tgcttgatac tgtgaagctg cttgatacag
     3361 ggcgttatct tagtttgata ctgtatttgc ttttgagtat cggtggaggc ctgcttttag
     3421 cttactattt ggggaggaag aaatggtaat cgtctatctt gcaatcgcct gtggcttggg
     3481 agcactcgta cggtatttct tttctcgcta taatcaagct tctaaattgc cactcggaac
     3541 gctcatagct aatcttttag gttgtttttt aattggagta ttctacaatc atgtagagtc
     3601 taaggaagta tatgctattc tagcaacagg attttgtggc ggtttaacaa ctttttcgac
     3661 cttgaatgac gaacttcaaa gactgctaag tgataagaag gtcttttatt cttacctgac
     3721 tttgacttac ataggtggtt tggttgcgat ttttttagga attttgctat aaatttcttt
     3781 acttttttgt gaaaatttgg taaactgtat cttgtgtgta atggacgcac aagaatacag
     3841 tttatccgct gaggaagttt cctcaagatt gacaaagata ggagaataaa atgaatccat
     3901 taatccaaag cttgactgaa ggtcaacttc gtacagatat cccatcattc cgtcctggtg
     3961 acactgttcg tgtacacgcg aaagttgtcg aaggtaaccg tgaacgtatc cagatttttg
     4021 aaggtgttgt tatcgcacgt aaaggtgctg gaatctcaga aaactacaca gttcgtaaaa
     4081 tctctaacgg tgtaggtgtt gagcgtatct tcccaatcca cactccacgt gttgaaaaaa
     4141 tcgaagttgt tcgttacggt aaagtacgtc gtgcgaaatt gtactacttg cgtgctcttc
     4201 aaggtaaagc agctcgtatc aaagaaatcc gtcgttaatt cgactaaaaa actctgcttt
     4261 tcagcagagt ttttatctag ctcccttagt tcaatggata taacaactcc ctcctaagga
     4321 gtagttgcag gttcgattcc tgcagggggc agtaattaga catcaaaaac cgctcagctg
     4381 agcggttttt atctattctt tccttttaca gtacccatgt acgaatagca tgggcaacgc
     4441 cggattcgtc atttgttttg gtgatgtatt tggcgatttt tttgatttct ggatttccgt
     4501 tttccatgac tacagggttg ccaacgactt ccagcatggc acggtcattt tcttcgtctc
     4561 cgatagccat ggtttcatct ttggttaatc ctagtttttc agctaagtga gtaatagctg
     4621 aacccttgtc tacattcttt ttaaggagtt cgaggtagaa aggagcagat ttgttgatag
     4681 agtagtgctc gtaaaattcg gctggtattt tttcaatcgc agcatcgagg atttctggtt
     4741 catcgatgaa catacacttg acaatttcct tgccagccat ttcttcaggg gtacggtaga
     4801 agataggcat gctgacgagg gttgattcgt gtacagtgta ttttccgata ttgcgatttg
     4861 cagtatagat accgtccttg gtaatggcat gcatgtggac accgagcttg cgactgagga
     4921 attccatatc cagataatcc tcataggtca aggattcgct gataatctca tggccagtag
     4981 cagtttcttg gacaagggca ccattgaagg tcaccacata gtcaccttgg tctctcaact
     5041 gcaagtcgtc cagaagtttg gcaacacctg cgatggggcg gccagttgca atcacgactt
     5101 tgacaccagc ttctttggca tcttggatgg cagaaaagac ttcaggagtg atttcctttt
     5161 ggctgttgac tagggttccg tcaatatcga cggcgattag tttaatactc ataaatttcc
     5221 tcttttagtt tttatttggg gtaaaatgat cgttctcaat caagtgtaaa aattgctggg
     5281 tgatgcttgc gaagatgctg ttttggtcta acatttcttt cgggaaataa aagcgattat
     5341 ctccgtggcg acttccagca agggattgga cgatagggga caggctagag agttctgcca
     5401 gttctccatt tttttgtaaa atctcaatct gtgtccttgg attttcagat tcgggacggt
     5461 aaatatcata agggaggtca aagttcttat gaatggcagt atagtagtcg ggatcaaagc
     5521 cgatgtcctc aaccaatttt ctcatgctag cgagttggtc ttggtcttct tgtgaaaagg
     5581 taatggattt aaagaccttg cggttgacaa agcgatgcga taaatctgca agaatcttgt
     5641 caggactggt catccagagt tggaagtagg tattcatcac accatcatcc agagccagat
     5701 agtcagtcaa ggtcacattt ttttcgaaga aaggcaggag gtgtggagaa gttcgggcaa
     5761 agaaatcctt gtcctcagga tagagttcct tggcgcgttt gagaagattc tgtaggagaa
     5821 cttccatggc gcgtgttgcg gggtggaaat aaacctgcat gtacatctgg tagcgactga
     5881 ggacgtagtc ttcgatggcg tgcatgccat tgcgctgaaa ggcgatacca ttttcgatag
     5941 gacgaatgac tcggaggatt cgagtcaggt caaattcccc ataggatgct cctgtaaaat
     6001 aggagtcgcg caagagatag tccatgcgat ctgcgtcaat ctgactagaa atgagctgca
     6061 cgacctgctt gttaggatag gtatggtcta tgacactggc aactttctct ggaaaatcgg
     6121 gcgctacttg tagcaagact tggtgaatct ctgtctcagg attttgaata atctcctgag
     6181 taatggcttc atggtctgta tcaaagagat tttcaaaagt atgggagtag gcaccatgcc
     6241 caaggtcgtg taggagagca gcggtcatgg tcaagagaga ctcggcagga ttccattcct
     6301 caggatattt ttcttcgaaa atctctgtga tgcgtcgtgc aatttcatag actcctagac
     6361 agtgagagaa gcgactgtgt tctccaccgt ggaaggtata actggaagtt cccagttgtt
     6421 tgatccggcg caaacgctga aattcttttg tattaatcaa gtcatagatg atttgattat
     6481 tgacatggat gtagttgtga acagggtcac ggaatacttt ttcgttcata tgaccattat
     6541 agcaaaatcc tgctcttttt ggtaaaatag taggacaaga gacaagaaag agggcctgat
     6601 gtgaagattc ggatgcgaaa tacaattcag tttgatgagc agttggaagt gattgaccag
     6661 ctttatgatg tggaagtgca tgaaaaagga gattatagct acctgctttt ctataatgag
     6721 gaaaaggaaa aagtagtgat taaatttcat ggtcaagaac ttatgatgag ccgattttct
     6781 aatcccaaga ccattatgcg ctttctaaag gatagcgata gtttagccta tattcctaca
     6841 cctatgggca tgcaggagtt tatcatccaa acgagccatt atcaagttga taggcaaaag
     6901 attgaactag attatcaact acaaaatcaa gagggacatc cttttgccag ctatcaattg
     6961 gaaattactt ggggctaggc tcatgtcttt ttcaaattta gctatctatc ttcttggatc
     7021 ggcttcctag tgtggtagat taggctaatt tttggtataa taaaagttat ggaaatcgaa
     7081 aaaaccaatc gtatgaatgc gctctttgaa ttttatgcgg cgcttttgac agataagcaa
     7141 atgaattata tcgagctcta ctacgctgat gattacagcc ttgctgaaat tgccgaggag
     7201 ttcggtgtca gtcgtcaggc tgtctatgac aatatcaagc gaacagaaaa gattctggaa
     7261 gattatgaga tgaaattgca catgtactcg gattatattg tccgcagtca gatttttgat
     7321 cagattttgg agcgctatcc caaggataac tttctgcagg agcagataga aattttaaca
     7381 agcattgata atagagaata agaggaagaa aaatggcatt tgaaagttta acagaacgtt
     7441 tgcagaacgt ctttaaaaat ctacgtaaaa aaggaaaaat ctctgaatct gatgtccaag
     7501 aggcaaccaa agagattcgc ttggccttgc ttgaagccga cgttgccttg cctgttgtga
     7561 aggattttat caagaaggtt cgtgagcgtg cagtcgggca tgaggtcatt gatacactta
     7621 atcctgcgca acagattatt aaaatcgttg atgaggaact gacagccgtt ttaggttctg
     7681 atacggcaga aattatcaag tcacctaaga ttccaaccat catcatgatg gttggtttac
     7741 aaggggctgg taaaacaacc tttgctggta aattggccaa caaactcaag aaagaagaaa
     7801 atgctcgtcc tttgatgatt gcggcggata tttatcgtcc agctgccatt gaccagctta
     7861 agaccttggg acaacagatt gatgtgcctg tctttgcact tggaacagaa gtaccagctg
     7921 ttgagattgt acgtcaaggt ttggagcaag cccaaactaa tcataacgac tatgtcttga
     7981 ttgatactgc gggtcgtttg cagattgatg agctcctcat gaatgagctt cgtgatgtga
     8041 aagcattggc tcaaccaaat gaaatcttgc ttgtcgttga tgctatgatt ggtcaggaag
     8101 cagccaatgt tgcgcgtgag tttaatgctc agttggaagt gactggggtc atccttacca
     8161 agattgatgg cgatactcgt ggtggtgctg ctctgtctgt tcgtcacatt actggaaaac
     8221 caatcaagtt cactggtaca ggtgaaaaga ttacggacat tgaaaccttc cacccagacc
     8281 gcatgtctag ccgtatcctt ggtatggggg atatgctcac tttgattgag aaagcttctc
     8341 aggaatacga tgaacaaaaa gcccttgaaa tggctgagaa gatgcgcgaa aacacctttg
     8401 attttaatga tttcatcgat caattagatc aggtgcaaaa tatggggccg atggaagact
     8461 tgctcaagat gattccaggt atggccaaca atccagccct tcaaaacatg aagatggatg
     8521 aacgccagat tgctcgtaaa cgtgccattg tgtcttcgat gacacctgaa gagcgtgaaa
     8581 acccagattt gttaaatcca agccgtcgcc gtcgtattgc tgctggttct ggaaatacat
     8641 tcgtcgaagt caataaattc atcaaggact ttaaccaggc taaacagctc atgcagggtg
     8701 ttatgtctgg ggatatgaat aaaatgatga agcaaatggg gattaatcca aataaccttc
     8761 ctaaaaatat gccaaatatg ggaggaatgg atatgtctgc ccttgaagga atgatgggac
     8821 aaggcggtat gcctgactta tcagctctcg gaggagcagg aatgccagat atgagccaga
     8881 tgtttggtgg cggtttgaaa ggtaaaattg gtgaatttgc catgaaacag tccatgaaac
     8941 gtatggctaa caaaatgaag aaagcgaaga agaaacgcaa gtaagacaag agattgatgc
     9001 aaatctcatt tgatgagaaa ataaagtaaa aatttctatg ataaaacgta cagtatcaag
     9061 gttttagaat atctgatatt atacgttttt tgattttaat aattttgtta agacactttt
     9121 attatggtat gcgaaaaatc cactcagctg tctgagtgga ttaggtgtat ttagactctt
     9181 agtctttatt ttcgtatggc aagatcaagt tcaagatgat tcctgtcatg gctgaaaggg
     9241 cagtacctga aagtgtaact ggaccaagtt taaggatagc tcctccaagt ccaagaacca
     9301 acatagcact tgcgatgatg aggtttcgca tttgagcgaa atcaacacgt tttttaatca
     9361 agactttcaa accattgctg gcgataaccc catagagaag gattgacata ccaccaagta
     9421 cagcgtttgg aatagttgaa atcaaggcag tgaatttacc aaggaagctg agggcaatcg
     9481 cgatgaaggc agcgttacgg ataactgaga cagaagcgat acgagtcata ccgataaccc
     9541 ctgtattttc tccgtaagtt gtattggctg gtccaccaag gaaggcagaa acagaagttg
     9601 cgataccgtc accaagaaga gtacgatgaa gacctggttc ttttaagaat tgacggccac
     9661 agatttgacc caaaacagta tggtctccga tatgttcaga aattgttacg atagcgattg
     9721 gcaagatagc gatggcttct ggaccaaagt aaagattgta ctctttaaag gcaccacctg
     9781 tgctaaatgg caagtagaaa ccaggaattt cgaaccagtt ggctttaaga actggtgtaa
     9841 agtcaaccaa gccaagagtt agtgcgaaaa ggtaaccacc gataatggca aagaggaatg
     9901 gaatgattcg taggaagcct tttccttttg tattgataaa ggcagcaatt aggaaagtaa
     9961 caacggctac cagagcattt ttccaatttc cgtctgctac aagacctgca ttggtaacag
    10021 ctgaacctgc aagtccaaga ccgataacga tgatcatagg accgataatg attggtggca
    10081 agagtttatc aatccatttt gttcctacaa atcggatgct ggtagcaaca aggacataga
    10141 ccaaaccagt caagataacc cctgtttggg cagcagatac atcacccccc atttctttca
    10201 tagccagtga catagctgtg ataaaggcaa atgaagaacc tagataaact ggaactttaa
    10261 aaccagtagc aatcatgtag atgagtgttc caacacctga agcaaaaagg gcaacagata
    10321 caggcattcc caaaatcaat ggtaccaaga tggtcgcacc aaacatggcg aaaacgtgtt
    10381 ggaagctaag gagaattcct tttccatccg aaggacgttg gtcaacgtct agtaacaaat
    10441 caacagttga ttcttgtttc atataaacct cacttttggg catcagaaaa gagcccatta
    10501 ttttacagca ataggccctc agagtaagac ttctctaaaa tctagacttt aaagagtttc
    10561 aaatattttt gcgtcttcgt cacctcacgg gatgacatta aaaacctttg cttaggatag
    10621 tatagcagaa aaaaatgatt ttgtaaatca ttttttcccg agcctagaaa taaaagagcg
    10681 aggctgattt tgtaaacatt tgtttactaa aaaatttaaa gtgggcgttt atttggcata
    10741 ccagccttac gtggatattt atttggtgtt tcttttttct tttctaccac tgtgatatag
    10801 cgcggatctc tattcggtag ggcgtagctg agattgtctt cgaccttact aaaaaggaga
    10861 ttgagggcat tcttagcttc taataattcc tcaggcgcat tgctggcctt gagtgccaat
    10921 agtttgccac caaccttaag gtagggaata gtcaattcag ataggacctg catacgggca
    10981 accgcacgag ctgttacaaa atcatattga gcacggaagt tcttgtcttg ggcaaaatct
    11041 tcggcacgtc cgtggtagaa atgaactccg ttcaaatcca gttcttgagc caagagttgt
    11101 aggaagttga tgcgcttatt gagtgaatca ataatggtca catctaactc cggatagaga
    11161 attttcattg gtagactagg aaatcctgcc ccagccccga tatcaagaag tttgatagtt
    11221 tcattgggaa tcaaaccttg aagaatgggt gcaatcgaat cgtaaaaatg tttgagataa
    11281 acttcttcct tgtccgtaat cgccgtcaaa ttaatcttct cattccactc gaccaagagc
    11341 tcaaaataac gttcaaattg ttctttttgc tggtccgaaa gtggaagatt ctgctcggca
    11401 agcaagttgt aaaatgtttt tggtttcata gttatttcct tctttagtat catcttattt
    11461 taccacaatc atagaaaatt tgatatactg gtactaatct aaaagcagaa aggacttata
    11521 ttatgacttg gattattctt ggagttttag ctcttgttgt tatttttgtg attgttagct
    11581 ataacggttt ggttaaaaat cgtatgcaaa ccaaggaggc ttggagtcag attgatgttc
    11641 agttgaaacg tcgaaatgac ctcttgccaa acttgattga gactgtaaaa ggttatgcca
    11701 aatatgaagg ttctaccctt gaaaaggtgg cagaactacg taaccaagtg gcggcagcga
    11761 cttcaccagc agaagctatg aaagccagtg atgccctcac tcgtcaggtt tcaggtattt
    11821 ttgcagttgc agaaagctat ccagatttga aagctagtgc taactttgtt aaattgcaag
    11881 aggagttgac aaacacagaa aataaaattt cttactctcg tcaactctat aacagtgttg
    11941 tcagcaacta caatgtaaaa ttagaaactt tcccgagcaa tattatcgct ggaatgtttg
    12001 gatttaaagc ggcagatttc cttcaaacac ctgaagagga aaagtcggtt cctaaagttg
    12061 attttagcgg tttaggtgac taagatgttg tttgatcaaa ttgcaagcaa taaacgaaaa
    12121 acctggattt tgttgctggt atttttccta ctcttagctc ttgttggtta tgcggttggt
    12181 tatctcttta taagatctgg acttggtggt ttggttatcg cactgattat cggctttatc
    12241 tacgctttgt ctatgatttt tcaatcgaca gagattgtca tgtccatgaa tggagcgcgt
    12301 gaggtggatg agcaaacggc accagacctc taccatgtag tggaagatat ggctctggtc
    12361 gctcagattc ctatgccccg tgttttcatc attgatgatc cagccttaaa tgcctttgcg
    12421 acaggttcta atcctcaaaa tgcggctgtt gctgcgactt caggtctact agctatcatg
    12481 aatcgtgaag aactagaagc tgttatggga catgaagtca gtcatattcg taattatgat
    12541 atccgtattt cgactattgc agttgccctt gctagtgcta tcaccatgct ttctagtatg
    12601 gcaggtcgta tgatgtggtg gggtggagca ggtcgcagac gaagtgatga tgaccgagat
    12661 ggaaatggtc ttgaaatcat tatgctagtg gtttccctac tagctattgt actggcacct
    12721 ctcgctgcaa ccttggttca gctcgctatt tctcgtcaga gggaatttct ggcagatgca
    12781 tctagtgtcg agctgactcg caatccccag ggaatgatta atgccctaga taagttggac
    12841 aatagcaaac ctatgagtcg ccacgtcgat gatgctagca gtgcccttta tatcaatgat
    12901 cccaagaaag gtggggggtt ccaaaaactc ttttataccc acccacctat ctcagaacgg
    12961 attgaacgtt taaaacatat gtaaaatgaa ggctggaaaa aagtctttaa aatctgaaaa
    13021 acgcataata tcaggtgtga aaacttgata ttatgcgttt tactatggga agatttactt
    13081 ctttttctcc taaaattgtg tttttgcccc acctatctgc tatgttgcaa atttataaat
    13141 cttctaaatt aactagcttc aaaccgtgtt ctgtcatgcg atagatgatc gagcagactt
    13201 cttttaagaa acgacggtca tgcgaaacag tgatgagacc gccaggataa gtggcaaaga
    13261 gttttctgat ttggggttga gaagtgggag aaaagtttcg tgtgggttca tccagcagga
    13321 gaaagtttgg tttgcgcagg actaaatcca aaagcaggag ttttccctgt tgtccgccag
    13381 ataaggagcg aatttgatgc tgcatttctg gataactgaa attgagacta gctaggtgag
    13441 attggatttt ctgtagttcc tctttttccc cagttttact gagataggct attggggata
    13501 aatccaattg cagttttttg tggtaatctt gtggcataaa accaagtgaa atctctcttt
    13561 tatcattcag aagtctctgt aacttggcta acagagttga tttcccaaca ccatttggcc
    13621 cgataatacc gattttttct tggccacgga cagttagttg tagtttttga accaaaactc
    13681 ggtcgtcaat ggacaaattt tctttttcca gttggactaa gactttagaa gctggtaatg
    13741 gttggatgtc tgaaaagaaa agttggattt gttcctcttc aagtggcttt tgagtcatgg
    13801 actgagctgc cttttcgtag cgtttttctt gtgagaggac agttttcatc tttttagcca
    13861 ataggcgacc ggcagtacta tctttggtag ctcgaagcgc agtttctaca ctttgcttga
    13921 ctctccgatg tttttccatg gttttatcgt aggctctttg gttgttagca gcttgctgac
    13981 tttgtttggc aaaattagcc tttctctgct cactatagct atcatagtct aaatgctcta
    14041 ctagcgtttc cgcttcttta cggtgtttga ccagtcgcaa gtgaacaata gtgtctgccg
    14101 tttcagaaag aaagtcttca tcatgggaaa tgaaaataac ggtttgcctg gtcttttgaa
    14161 tctggccttt tagccaatca actgtctcaa ggtctaggtc atttgaaggt tcatctaaaa
    14221 atagaatctc aaagggtttg gctaactcat ggataagctg aattttcaaa gcttcgcccc
    14281 ctgatagatt gccaatcttt tggtcacttg cgaaacgatt gctatcaaaa tgcaattcct
    14341 ccgccaaacg atagaggata ctgtagtcta aatcaataga atctaaaaag aagtagtcgt
    14401 gtaaagtttt ctttttcagc tcctcgggaa gtttttgagg aatgtaggcc agtgactgat
    14461 agtcagattg gatgtttccc ttgatagtga aatcagacaa agcttccccc attaaaattt
    14521 taagtaaggt tgatttgcca tttccttctt caccaataat agcaaccttt tccccgtctt
    14581 ggatggtcat gcttaggtca gatacaagat ctcgtaaatc tttgttttgt gtgatggtta
    14641 gatgattgat ttttatcata tgatttccct ttctagaatg ttcatagtgc ataacaaaaa
    14701 gctctacttt acctagtaga gcccaaagtc tatgtcaaaa aaactctatt aacatgccag
    14761 aaaaagagca taaaagagca cacaaaaaga gacaaaaaca agatctacta aataaaggtt
    14821 ctttatttga tagacttagt tgttcatgtc tcccttacct ccgagtatta gtatctttat
    14881 attatacctt taaggaaatt ttgtcaacag aaaactggaa attctatgct ctaaaatcca
    14941 aaaacaggtc catagagact tagtacgtgt ttgacatgct cgtaatggaa tttatcatag
    15001 tttcgccaag cagtgcgtgc ttgatcgttt agttttaggc tacccagtcc aatcagaaga
    15061 ctggtgggtt ggtaaaattt ctcaagatca attaagatac tgttgttcaa cttttccgtt
    15121 ttttgagctc ttgcatagta gtgtttagca gctcttgtag tcgaatatca tctgccgtac
    15181 cctgtttaca gttttggtag cttttattta acttggatag gagttgaatg agtttcttgt
    15241 cttccatgtg agcatcctcc ttgatctgat gtactctttc cctatagtat agcactaaaa
    15301 aacaaaactg tcatagtaaa tatgttaaaa ttttgaattt taattacaaa gtttggtgtg
    15361 ctgatttgta gccttttcat ggtataatca agagagtaaa tctttatgga ggaagtatga
    15421 aattaaatat tcaagaaatt cgtaagcagt ctgaaggttt gaactttgaa caaacgttag
    15481 atttagttga tgacctgcgt gcacgtaatc aagaaatttt agatgtaaaa gatatccttg
    15541 cagttgggaa agtacaatat gaagaccgta tgtatttctt agattatcaa ctatcttata
    15601 ccattgttct tgcttcgagt cgcagtatgg agccagttga gttagttgaa tcttatccag
    15661 tcacggaagt tttcatggaa ggcgcaacta accatctaaa tcaagaaatt ttagatgatg
    15721 acttggtctt gcccatcgaa aatgaggcgc ttgaccttgc tgagagtgta tcagacaata
    15781 tcttgctaaa cattcctatc aaggtcttga cggctgaaga agaagctggt caaggatttg
    15841 tctcaggaaa tgactggcaa atcatgacag aggaagaata ccaagctcaa caagcagtaa
    15901 agaaagaaga aaacagtcct tttgctggct tacaaggact atttgacgga gatgaataat
    15961 ggtcttgtca aaaaaacgag cacgaaaggt gctagaagaa atcattgccc tcttcccaga
    16021 tgccaagctt agtcttgatt ttaccaatca ttttgaactc ctggttgcgg tcatgttatc
    16081 agcccagacg acagatgcag cggtaaataa ggccacacca ggtctctttg ttgcctttcc
    16141 aacaccccaa gctatgtctg tagcgacaga gagtgagatt gcttcacata tttctcgctt
    16201 gggattgtat cggaataaag ctaaattcct taaaaaatgt gcccaacagt tactagacga
    16261 ttttgatggt caagtccctc agacacgtga agaattggag agtttggcag gtgttggtcg
    16321 caagacagcc aatgttgtca tgagtgtagg atttgggatt ccagcctttg cagtggatac
    16381 tcatgtggag cgtatttgca aacaccacga tattgtcaaa aaatcagcga cgccacttga
    16441 ggtggaaaag cgggtcatgg atatcttgcc gcctgagcag tggttagctg cccatcaggc
    16501 catgatttat tttggaagag ccatttgcca tccaaaaaat ccagagtgtg accagtaccc
    16561 acaattatat gattttagca atttgtaaga tgctacattg aaactttttg cttgatttta
    16621 agcatgcttt gtgctatagt aaatgataac taaatattcc tttaaacctg tcccgtgagg
    16681 caggcaagga gcgcgataaa gtagaagggt gaagtctata ctgtgtgcag tagggctcgc
    16741 ttggctataa atttcaagtc tccttgttta aggagacttt tctttttgga ggtttgtcat
    16801 gaaaacaaaa gaagttgtag acgaattgac cgtcaaacga gcgattacgc gtattactta
    16861 tgagattatc gaacgcaaca aagatttgaa taagatcgtc ttggctggta ttaaaactcg
    16921 tggtgtcttt attgcccacc gaatccaaga acgtttgaag cagttagaaa atctttcagt
    16981 tcctgttgtg gaattggata ctaaaccttt ccgtgatgat gttaaaagtg gagaagatac
    17041 ttctttggtt tctgtcgatg tgacagaccg cgaagttatc ttggtggatg atgtgctcta
    17101 tacaggtcgt accatccgtg ctgctattga taatattgtc ggtcatggtc gtcctgcgcg
    17161 tgtgagttta gcagttctag tcgatcgtgg acatagagaa ttgccaatcc gtccagatta
    17221 cgttggaaaa aatatcccaa ccagtcgttc tgaagaaatc atcgtagaga tgacagaact
    17281 tgatgaccaa gacagagttc tgattactga agaagcttag aaagttaaag gagtagccat
    17341 gtcagaaaat caacaagcat tgaaccatgt ggtgtccatg gaagacctca ctgtcgatca
    17401 agtaatgaaa ttgatcaagc gaggaattga gtttaaaaat ggagcccagc ttccctatga
    17461 agaccatccg attgtttcca atctcttctt tgaggattct acacggacac ataagtcctt
    17521 tgaagtcgca gagattaaac ttggattgga acgacttgac tttgatgtga agactagttc
    17581 ggttaataag ggtgagacac tttatgacac cattttgact ctgtctgctt taggagtgga
    17641 tgtctgtgtg attcgccatc cagaggtcga ctactataga gaattgattg ctagtccaac
    17701 gattacgact tccatcatca atggtggaga tggttcgggc caacacccta gccagagctt
    17761 gcttgatttg atgaccattt atgaggaatt tggccacttt gagggtctta aagttgctat
    17821 tgcaggtgac ttggaccact cacgcgttgc caaatccaat atgcagattt tgaaacgctt
    17881 gggatctgaa ctctttttcg ctggacctga ggaatggaga agtcaagagt ttgcagacta
    17941 tggacagttt gtaaccattg atgaaatcat tgatcaggtt gatgtcatga tgtttctccg
    18001 tgtgcaacac gaacgccatg atagtggagc tgtattttca aaagaagact accatgccca
    18061 acatggcttg actcaagaac gttacgatcg tttgaaagaa acagcaatcc tcatgcaccc
    18121 agctccaatc aatcgtgatg tagaaatagc agaccacttg gttgaagcac caaaatcacg
    18181 gattgtccaa caaatgacca atggtgtctt tgttcgaatg gcaatcttag aatccgtact
    18241 agcgagtaga aacgccaact aaaacacaag acgttaaaag acgccagtga gcgtccacga
    18301 gaggtgaaaa tatgacaaaa agaattctag tattagaaga tggcacagtt tttgaaggca
    18361 aggccttcgg agcagatatt gatgtaacag gcgaaatcgt ctttaataca gggatgaccg
    18421 gctaccaaga atccattaca gaccagtctt ataatggaca aatcttgacc tttacttatc
    18481 ctttggtagg aaattatggt attaatcgtg atgattatga atccattatt ccaacttgta
    18541 agggagtcgt tgtttttgaa gaagcgcgta gagctagcaa ctggcgcaac caaatgacct
    18601 tggatgaatt tttgaaagcc aagaaaattc cagggatttc aggaattgat acgcgtgctc
    18661 ttaccaagat tatccgtaag catggtacta tgcgtgcaac cttgacccat gttggggaca
    18721 gtatggacca tgtgacggac cagctccaag caacagtttt accgacagac aatatcaaac
    18781 aggtttctac taaaacgtct tatccagctc caggagttgg tttgagcgtg gtgctagtgg
    18841 actttggtct caagcactca atcctacgtg aactttctaa acgcaactgt aacgtgacag
    18901 ttgttcctta ttcgacaacg gctgaagaaa ttctccatct caatcctgac ggagttatgt
    18961 tgtcaaatgg tccaggtaac ccagaagacg ttccacaagc actcgacatg attcgcggtg
    19021 tacaaggaaa aattccaatc tttggtattt gtatgggaca ccaactcttt gcaatggcaa
    19081 acggggctaa gacctataag atgaagtttg gccaccgtgg attcaaccat gcggtacgtg
    19141 aaatcgcaac aggacgagta gacttcacca gccagaacca tggttatgca gtcagccgcg
    19201 aggatttgcc agagcacttg attattaccc acgaagaaat caatgacaag tcagtggaag
    19261 gtgtgcgcca cagataccaa cctgctttct ctgttcaata ccacccagat gcagctcctg
    19321 ggccacacga cgctagctac ctatttgacg agtttatcga gatgatggaa gtttttaaac
    19381 aatcaaacta agaacgagta aaagctcgtc ggagaattta tgtaactgaa cacggacgga
    19441 aagctcagaa tgtagagaaa ccaacttgga ttgtcaatcc ttccttgatt tcctaaaatt
    19501 caatcgcttt ctattcgtcc tttgtatctt atttaatgtc gccctgagag gcgacgaata
    19561 gaaaggagaa gggtgctccg tatttgctga actgaatacg ggctacggac ggtgctaaaa
    19621 agatagttat tcctagaact gacagttctt cgtcataact cctattttag ctttgtccgc
    19681 ttgacaccct taatatctta tatatgccta aacgtactga tattcaaaaa attatggtga
    19741 ttggttctgg tccgattatt attggtcagg ctgctgagtt tgactacgct gggactcagg
    19801 cttgcttgtc gttgaaagag gaaggttatg aggttgtttt ggttaactca aatcctgcaa
    19861 ccatcatgac ggacaaggaa attgctgaca aggtttacat tgaaccgatt acacttgagt
    19921 ttgtgacacg tattcttcgt aaggaaggtc cagatgcctt gctaccaaca ctcggtggtc
    19981 agacaggtct caacatggcc atggaattat ctaaaaatgg tatcctagac gagcttggcg
    20041 ttgaacttct gggtaccaaa ttatctgcca ttgaccaagc ggaggaccgt gacctcttta
    20101 aacaattgat ggaagagctt gaacaaccca ttccagaatc tgaaattgtt aacacagtag
    20161 aagaagcagt tgcctttgca gcgacaattg gctacccagt catcgttcgt ccagccttta
    20221 cccttggtgg tactggtggt ggtatgtgtg ccaacgagaa agaattgcgt gaaatcactg
    20281 aaaatggttt gaaattgtca cctgttaccc aatgtttgat tgagcgttcg attgccggtt
    20341 tcaaggaaat tgaatacgaa gtgatgcgtg actcagctga caatgccttg gttgtttgta
    20401 acatggaaaa ctttgaccca gttgggattc acacagggga ttccattgta tttgcccctg
    20461 cgcaaaccat gtcagactat gaaaaccaaa tgcttcgtga tgcgagcttg agcattattc
    20521 gtgccctcaa gattgaaggt ggatgtaatg ttcagctagc tcttgatcct aacagcttca
    20581 agtattatgt tatcgaagta aaccctcgtg tatcacgttc gtcagccctt gcttctaaag
    20641 cgacaggata tccgattgct aagttggctg ccaagattgc agtaggtttg accttggatg
    20701 aggttataaa cccagttaca ggttcaacct atgccatgtt tgaaccagcc cttgactacg
    20761 tagttgctaa gattccacgt ttcccatttg acaagtttga aaagggtgaa cgccgtctcg
    20821 gtacacaaat gaaggcgact ggagaagtca tggcaatcgg tcgtaacatc gaggaatctc
    20881 ttctcaaggc ctgccgctcc cttgaaattg gggtgcacca caatgaaata cctgaacttg
    20941 cagcagtttc tgatgatgcc ttgattgaaa aggttgtcaa agcccaagat gaccgtctct
    21001 tctacgtatc agaagccatt cgtcgtggct acacacctga agaaattgca gagcttacaa
    21061 aaattgatat cttctatctt gataaactct tgcatatctt tgaaattgag caagaattgg
    21121 gtgctcatcc acaagattta gaagttttga aaacagccaa acttaatggt ttttcagacc
    21181 gtaagattgc tgaactctgg ggaacgactg atgataaagt tcgccaactt cgcttggaaa
    21241 acaagattgt tccagtttat aagatggtag acacttgtgc ggcagagttc gactccgaaa
    21301 cgccatattt ctattcaacc tatggttggg aaaatgaatc tatcagatct gataaagaat
    21361 ctgtacttgt cctaggttca ggtccaatcc gtatcggtca aggggttgag tttgactacg
    21421 caaccgttca ctctgttaag gctatccagg cagctggtta tgaagctatc atcatgaact
    21481 caaacccaga gaccgtttca acagacttct ctgtatcaga taagctttac tttgagccat
    21541 tgacattcga agatgttatg aacgtcattg acttggaaca accaaaggga gttatcgttc
    21601 agttcggtgg tcaaacagcc atcaaccttg cggagccatt ggcaaaagca ggtgtgacca
    21661 tccttggtac acaggttgct gacctagatc gtgccgaaga ccgtgacctc ttcgagcaag
    21721 ctctcaaaga attggatatt ccacagccac caggacaaac ggctaccaat gaagaagaag
    21781 cagctcttgc agctcgtaag attggcttcc cagttcttgt ccgcccatct tatgtacttg
    21841 gtggacgtgc catggaaatc gttgaaaacg aagaagacct tagttcttac atgcgaactg
    21901 ctgttaaggc tagtccagac cacccagttc ttgtcgactc ttatatcgtt gggcaagagt
    21961 gcgaagttga tgccatttca gatggtaaaa atgtactcat tcctggtatc atggagcata
    22021 tcgaacgtgc tggtgtccac tcaggtgact caatggctgt ttacccacca caaaccttgt
    22081 cgcaaaaggt tcaggagacc atcgcagact acaccaaacg cttagcaatc ggtcttcact
    22141 gccttggaat gatgaacatc cagtttgtta tcaaggatga aaaagtctac gttattgagg
    22201 tcaatccacg tgccagccgt actgttccat tcctatctaa ggtaaccaat attcctatgg
    22261 ctcaagtagc gaccaaactc attcttggtc aaagtttatc agaacttggc taccaaaatg
    22321 gactttaccc tgaaagcacc cgtgttcata tcaaggcacc tgtcttctcc tttaccaaac
    22381 tagctaaggt agacagcttg ctcggtcctg aaatgaagtc aacaggtgaa gttatgggtt
    22441 ctgatgctac tttggaaaaa gctctctata aagcctttga agcttcttat ctacacttgc
    22501 caacttttgg taatgtcgtc tttaccatcg ctgatgatgc caaagaagaa gccttgaact
    22561 tggctcgtcg tttccaaaac attggttatg gaatccttgc gacagaaggg acagcagcct
    22621 tctttgctag tcatggcttg caagcccaac ctgttggtaa gattggtgat gacgataagg
    22681 atatcccaag ttttgtacgc aaaggaagaa ttcaagctat cattaacaca gtaggaacaa
    22741 aacgaactgc tgacgaagat ggtgagcaaa ttcgtcgttc agccattgaa cacggggtgc
    22801 ccctcttcac agcgctagat acagctaatg ccatgcttaa ggtacttgaa agtcgtagct
    22861 ttgttacaga agcgatttaa atagaaaaaa taagatatag cagcctgaat ttagaacagt
    22921 acacagggat actaaaatat ttctagaaat taatttgaat tttctaattg atttgttcgc
    22981 atcttatttc aatctactat agaaaaaagt ctttaaaatt ataaaacgca tcatatcaag
    23041 gtttttcaaa aaacttgata tgatgcgttt tattgtggga atatgtattt cattttctac
    23101 taaaattatg tttttgaata acctctatct tagtagtctg tataatcccc ctcaatcagt
    23161 ttttgcgata agctttaatg ctatgactat accactcttg catttctttt gatggtggtg
    23221 tcatataatc gccatacatc tgggttaaaa attggtcata ttttttggga acaggcaaca
    23281 tacggccctc aaactcagtt aaaatcagtt ctttaaaggt atcaactggg aagatttctt
    23341 tcatcccttc cttaccgatc ccaactcctc cttcatattg aggagtgttg gttacagcat
    23401 ttttgactag ttgatcaatt ttcttgtaaa agtagcgagg attgacaaat cggagagcgt
    23461 accagctaca taatctaaga aaatctttta gtttgctatc accgtgaact gctcgtgatt
    23521 ttttgatata agctagttga cgaagagcca catacttata gctcttgtcg acaatgctca
    23581 agtctgtaaa tcgatcaatt gggaagacat cgatgaaaag gctggtatca tgacgcttgt
    23641 acttaacatg gtcttctata acagtagaag tgtccaaaat cgatgcgaaa ttatggaagt
    23701 accaagaaga tgtatcgtag gaaagaacct tgtagcgagg gtgattttct tcttcaataa
    23761 tcttcagtaa acgctcataa tcctcacgat aaagggaaat atcaatatca tcatcccaag
    23821 gaatcatacc tttgtggcgg atggctccaa gcatggttcc ataactgaga aaataaggaa
    23881 tatcatgttt cttacaagtc tcatcaatat agtccagcag ggctagttga atttctttaa
    23941 tttctttttt ttctaaatat tgcatcctaa tcctccaatt tataagcgtg aaattcatga
    24001 ctgtagaagc gtttttcttc tggtggtagg gtcatataat ctccataaaa ttgtgtcaaa
    24061 atagtatcaa atttttcagg tgcaggaagg cttaaattct caaaggttaa atcgattgtt
    24121 ttatcaaagg taccacttgg gaagacttcc ttttccttaa attttgaagg aataaaagcc
    24181 atatactgcc cattttcacg actatatttt tgaatttctt tctcgatttt atttgcaaaa
    24241 taacgaggag aaaccggtcg aaggagcaac caaaaggctg ttcgtatcca atcttttaaa
    24301 aggctatcct tatagacaat atttttatgt ttactgaaag acagcagttt gaagctttcc
    24361 agtttataac aagtatcaat gaccttagga tcatcaaagc gatctatagg gaaaatatcg
    24421 ataaagatac cagactcata ggtttttgta tttcgagtat caataatttt agtcgtactg
    24481 tcggttatct tgataaagtt gttaaagtag ttcttatcag tttctaagga taggagctta
    24541 tacttgcttt tttccttttg aaaaatgtta ataaatcgtt ggtagtcttc tctaggcatg
    24601 gacagatcaa tatcgtcgtc ccaagggata aagccctcat gtcgaaccgc cccaatcaga
    24661 gtaccgtagt taataatata gttgatattg tgctttttac agagagtatc aatataatcc
    24721 aaaatttcta attcaatttg tttggcatct tcaatggtta gttgtttcat tttaaactcc
    24781 tatgattttt tgaatttatt ttttaaggct aggacatggt ttaaaaattc atagaaaatg
    24841 ctatcttttg tgaagacaag tagactaata taagagatag ctgataacaa gacaatcaaa
    24901 ccagtattaa tcaaaaatgg caaattaatg accatatcca caggatacac gaaattaatc
    24961 aggaaataaa ttgctacaaa ggaaagtgaa aagagagagt atcgaacagt atagctaaag
    25021 atatgtccca agtggatgag ttgttttcta tggatgaaaa tgatatagaa aacaagtaga
    25081 gaagtctctg ataacatagt tgtcagtaag tagtattcgg gagccacgat atggttgaaa
    25141 aagaggagac tattcaagcc caaattgagt agcccagcaa agactgtata gactgtgata
    25201 cgtttttcat agccattggt aaagagaatt tgggaaccaa gaatggtatc taaggccagg
    25261 ataatcgtac gaaaagcgaa gagagaggtc aagatgccgc ctccgatata tttttcacta
    25321 ccgtaaagta ggatggcatt tggtcctaaa accatgagtc caaaactcag tggaatgata
    25381 aagaagttaa agattcgact acctctatta accagagaaa cataggcttc tttgtctcct
    25441 ttccccagat agtaactgag acgaggcaca ctcactccaa ttgcacctgt tacaacccca
    25501 gctataacgg tcacaattcg ctgagctatg gtatagtaac taacgttgac atcaatccct
    25561 gttttaacga ggaagaggcg atctaaaaaa gtgaagagca tattggcatt ggcaaagact
    25621 aacatggctg tcagagggag aaagagtggt ttaaaatcac ttaggtgaat cttaacaagt
    25681 ttgatgtctc ttttaatcca aaaataacta atcaggtagt taatcagcgt cgataaactc
    25741 atcacaagtg tatagacaac aatatcgtgt tcatttttaa caaataagaa aatagagacc
    25801 agcatcagga tacggatgaa ggcagttttg taaaagagaa aactgtaatt ttccagagct
    25861 tcattgaccc attcgattga aaaaatctgg gcaatgagtt gaatccccat aacaaggtag
    25921 acctttttga cgattggatt atcagtaaag aagagaggat aggctaggat atagacagca
    25981 gtggtcaaaa tcgtacaagc gatgcacaaa taaaaaagac tagaaaaggt tctgttaaga
    26041 tcttttttgt tatccttgac attactgata gcccttaaac cgtagttata gacaccataa
    26101 gttgcaaagg gcaagaaaaa tgacaaaata gtgtcgactg agttgaagta accatagtca
    26161 gttcggtcca agacacgcgc gacataggtt ccagttagga tgggaaaaat aatattcaag
    26221 acacgaattc ccatgtaaga tagagcattt aattttatac ttttcattca atttacctcg
    26281 tttttcatta tatcataaag ttagctaata agaaatgaag ggcagtaagt caagtaatca
    26341 ctttgaagtt tcaaatctta agttttaagt tttctttaag gaaagtatat tattctgaag
    26401 gactctaaaa tttcgcagcc atttattagt aattgctaca gaattcctag tcattactag
    26461 aaatggacta gtttctttga ataatagaac tgcataattc tcctattcta gaaggggagg
    26521 accagtattt cttttatgat aggactagat tgtggtataa tagagagaat aagttttttt
    26581 agtaagacaa aggagaaaat agatgattta tgcaggaatt cttgccggtg gaactggcac
    26641 acgcatgggg atcagtaact tgccaaaaca atttttagag ctaggtgatc gacctatttt
    26701 gattcataca attgaaaaat ttgtcttgga gccaagtatt gaaaaaattg tagttggtgt
    26761 tcatggagac tgggtttctc atgcagaaga tcttgtagat aaatatcttc ctctttataa
    26821 ggaacgtatc atcattacaa agggtggtgc tgaccgcaat acaagtatta agaaaatcat
    26881 tgaagccatt gatgcttatc gtccgcttac tccagaggat atcgttgtta cccacgattc
    26941 tgttcgtcca tttattacac ttcgcatgat tcaggacaat atccaacttg cccaaaatca
    27001 tgacgcagtg gacacagtgg tagaagcggt tgatactatc gttgaaagta ccaatggtca
    27061 atttattaca gatattccaa atcgtgctca cctttatcaa ggacaaacac ctcaaacatt
    27121 ccgttgcaag gacttcatgg acctttatgg atctctttct gatgaagaga aggaaatctt
    27181 gacagatgca tgtaaaatct ttgtgatcaa aggaaaagat gtggccttgg ccaaaggtga
    27241 atactcaaat ctgaagatta caaccgtaac agatttgaag attgcaaaaa gtatgattga
    27301 gaaagactag taaaatgatt aatcaaattt atcaactaac taagcctaag tttatcaatg
    27361 tcaaatatca ggaagaggct attgaccaag agaatcatat ccttatccgt cccaactaca
    27421 tggctgtctg tcatgcggat cagcgttact atcagggaaa acgtgatccc aagattttga
    27481 ataaaaagct tccaatggca atgattcacg agtcatgtgg aaccgtcatt tctgacccga
    27541 ccggaaccta cgaggttggt caaaaagttg tcatgattcc caatcagtct cctatgcaga
    27601 gtgatgaaga attctatgaa aactacatga cagggaccca tttcttgtct agtggatttg
    27661 atggctttat gagagagttt gtttctctcc ctaaagatcg tgtggtggct tatgatgcta
    27721 ttgaagatac ggttgcagcc attacagagt ttgtcagtgt gggcatgcac gctatgaatc
    27781 gtctattgac tcttgctcat agcaagcggg agcggatcgc cgttattgga gatggaagtt
    27841 tagcttttgt ggttgccaat attatcaact atactttgcc agaagcagag attgtggtta
    27901 ttggtcgtca ttgggaaaag ttggaactct tctcatttgc caaagaatgc tatattacgg
    27961 ataatattcc tgaagatttg gcctttgacc atgcttttga atgttgtggt ggtgatggta
    28021 ctggaccagc tattaatgac ttgattcgct acattcgtcc tcagggaacg attctcatga
    28081 tgggagttag cgaatataaa gtcaatctca atactcgcga tgccttagaa aagggcttga
    28141 ttttggttgg gtcatctcgt tctggtcgca ttgattttga aaatgctatc caaatgatgg
    28201 aagtcaagaa atttgccaat cgtcttaaaa atatccttta tctagaagaa cctgtaagag
    28261 aaattaaaga tattcatcgt gtctttgcaa ccgatttaaa cacagccttt aaaacagtgt
    28321 ttaagtggga agtataagta ctggaggtta attgtggaga aaatcattaa agaaaaaatt
    28381 tcttccttac ttagtcaaga agaggaagtc ctcagtgttg aacaactggg tggaatgacc
    28441 aatcaaaact atttggccaa aacaacaaat aagcaataca ttgttaaatt ctttggtaaa
    28501 gggacagaaa agcttatcaa tcgacaagat gaaaagtaca atcttgaact actaaaggat
    28561 ttaggcttag atgtaaaaaa ttatcttttt gatattgaag ctggtatcaa agtaaatgag
    28621 tatatcgaat ctgcgattac gcttgattca acgtcaatca agaccaagtt cgacaaaatt
    28681 actccaatat tacaaactat tcatacgtct gctaaggaat taagaggaga atttgctcct
    28741 tttgaagaaa tcaaaaaata cgaatccttg attgaagaac aaattcctta tgccaactat
    28801 gaatctgtta gaaatgcagt cttctcctta gagaaaagac tggctgactt aggtgttgac
    28861 agaaaatctt gtcatatcga tttggtgcct gaaaacttta tcgaatcacc tcaaggacga
    28921 ctttatttga ttgactggga atattcatca atgaatgatc caatgtggga tttggctgcc
    28981 ctctttttag agtctgaatt cacttcccaa gaggaagaaa ctttcttatc tcactatgag
    29041 agtgaccaaa caccggtttc tcatgaaaag attgctattt ataaaatttt acaagatact
    29101 atttggagtc tatggactgt ctataaggaa gagcaaggtg aagattttgg tgactatggt
    29161 gtgaatcgtt accaaagagc tattaaaggt ttggcttctt atggaggttc agatgaaaag
    29221 taaaaacgga gttccttttg gccttctctc aggtattttc tggggcttgg gtctaacggt
    29281 tagtgcttat atcttttcga tttttacaga tttgtcaccc tttgtggtgg ctgcaactca
    29341 tgattttttg agcatcttta tcttactagc ttttctcttg gtaaaagaag ggaaagttcg
    29401 cctctcaatt ttcttaaata ttcgcaatgt cagtgttatc ataggagcct tgctagcagg
    29461 ccctatcggt atgcaggcca atctttatgc agttaagtat atcggaagtt ctttagcttc
    29521 atctgtatcg gctatttacc ctgcgatttc agttctattg gctttcttct ttttgaagca
    29581 caagatttcg aaaaatactg tatttgggat tgtcttgatt attggaggga ttattgccca
    29641 gacctataag gttgaacagg ttaattcttt ctacattggg attctttgtg ctttggtttg
    29701 tgctattgca tggggaagtg agagtgttct tagctctttt gccatggaaa gtgaattgag
    29761 tgaaatcgaa gccctcttaa tccgtcaagt aacttcgttc ttgtcctatc ttgtgattgt
    29821 gctcttctct catcagtcat ttgttgcagt agccaatgga caattgctag gtctcatgat
    29881 tgtctttgta gcctttgata tgatttccta tttggcttat tatatcgcta tcaatcgctt
    29941 gcaaccagcc aaggctacag gcttgaacgt gagctatgta gtatggactg tcttgttcgc
    30001 agctcttttc ttgggaacat cattagatat gctgaccatt atgacgtcac ttgtcgtcat
    30061 tgctggagtt tatattatta ttaaagaata aaggagattc gtgtgaaagc cattatctta
    30121 gcagcgggat tgggaactcg cttgcgtcct atgactgaaa atacccctaa agccttggtt
    30181 caggttaatc aaaaaccttt gattgaatac caaattgagt ttctcaaaga aaaaggaatc
    30241 aatgacatca tcatcatcgt tggttatctt aaagaacaat tcgattattt aaaagaaaaa
    30301 tacggtgttc gcctcgtttt caatgataaa tacgctgact acaataactt ttactctctc
    30361 tatcttgtaa aagaagaatt ggccaacagc tatgttattg atgctgacaa ttatctcttt
    30421 aaaaatatgt tccgcaatga tttgacacgt tcgacttatt ttagtgttta tcgtgaagat
    30481 tgtaccaacg aatggttctt ggtctatgga gatgactaca aggttcaaga cattattgtt
    30541 gatagcaagg caggtcgcat ccttagtggt gtatccttct gggatgctcc aactgcagaa
    30601 aagattgtca gctttatcga caaggcttat gcaagtggtg aatttgttga tctctactgg
    30661 gacaatatgg ttaaggataa tatcaaagag ctagatgtct atgttgaaga attagaaggc
    30721 aatagcattt atgagatcga tagtgtccaa gactatcgta aattagaaga aattcttaaa
    30781 aacgaaaatt aaagattcca acatctgaca aaatagtcgg atgttttttg attttttacg
    30841 aatagataga tgagtagaaa aagaaatgga gttatttatg aaaatcacaa actatgaaat
    30901 ctataagtta aaaaaatcag gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg
    30961 tgaaaatgtt gatcaggagc ttttgttggg tgatattgca gatatctcag gttgccgtaa
    31021 tccagccgtt tttatggaac gttattttca gatagacgat gcgcatttgt cgaaagagtt
    31081 tcaaaaattt ccatctttct ctattttaga tgactgttat ccttgggatt tgagtgaaat
    31141 atatgatgcg cctgtacttt tattttacaa gggaaatctt gacctcctga aattcccgaa
    31201 ggtagcggtc gtgggcagtc gtgcttgtag caaacaggga gctaaatcag ttgaaaaagt
    31261 cattcaaggc ttggaaaatg aactggttat tgtcagtggt ctggccaagg gcattgacac
    31321 agcagctcat atggcagctc ttcagaatgg cggaaaaacc attgcagtga ttggaacagg
    31381 actggatgtg ttttatccta aagccaataa acgcttgcaa gactacatcg gcaatgacca
    31441 tctggttcta agtgaatatg gacctggcga acaacctctg aaatttcatt ttcctgcccg
    31501 taatcgcatc attgctggac tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc
    31561 aggtagtctc attacgtgtg agcgagcaat ggaagaagga cgcgatgtct ttgctattcc
    31621 tggtagcatt ttagatggac tatcagacgg ttgccatcat ttgattcaag aaggagcaaa
    31681 attggtcacc agtgggcaag atgttcttgc ggaatttgaa ttttaaaaat gacctaagct
    31741 agaattctaa gaaaaaatca attttaagag aaaatgaacc caacatttcc ataataaaac
    31801 gcatattagc aagtttttaa cacttgataa tatgcgtttt ttctaagtgg attagtagag
    31861 tagaggattt ttctcatata atactcttcg aaaatctctt caaactacgt cagcttccat
    31921 ctgcaacctc aaaacagtat tttgagctga cttcgtcagt cttatctaca acctcaaagc
    31981 agtgctttga gcaacctgtg gctagcttcc tagtttgcgc tttgattttc attgagtata
    32041 agggaaagta tagtgaattg aaataagatg tgaacaactc tatcaggaaa gtcaaattga
    32101 tttatagaaa tattttagca gccaaggtgc actgttatag attcaataca ctataattta
    32161 gtgtaattga gaaaggagaa atgattgtga ttgatgttgg ctaggttatg ttcaatgatt
    32221 cctaccgtct caaatcttgt cagtaaggaa aaataaattc ttcaaaagta gagattacaa
    32281 ggcttgttta agaaagaatt caaagacctt gacaaataaa aataaaatgg ttattataaa
    32341 aaaatggtct gaaatagatg atgatacttt tcgaaaatct cttcaaacta cgtcagctca
    32401 gctttgcctt gctgtgtttt gagcaagcta cggttagctt ccgagtttga ttttcattta
    32461 ctagaaatga aactgatgag agatatcagt agacatttga gtcaggatat tatggaaaat
    32521 gataaaaaga gctcgtgaga ttggcatatc agactactaa agtattgagt ttgttaggat
    32581 tttagcgact agttagctgg gaaaggaaga tatttgtgac aaataataaa ctgtattcgt
    32641 tgatagaatt tagaaataaa atatatgaag aattagaact ttccagaagt gatttagcga
    32701 ttttactatg tgccatgctt atcgcctcta tcggattaaa tatggattcg actcccgtga
    32761 ttattggagc catgttaatc tctcctttga tgacacctat tctgggagtg gggctctctc
    32821 tagctatatt tgattttaaa ttgttaagaa aatcttttaa aatattagct attcaaattc
    32881 ttgccagttt aatagcttca acactttatt tttatctttc tcccatttcg tatgctagtt
    32941 cggaaattgt tgctagaacc tctccgacta tttgggatgt tctcattgct tttgtaggag
    33001 ggatagcagg tattattggt gctaggaaaa aagagaccaa taatattgtt cctggtgttg
    33061 ctattgcaac cgccttgatg cctcctcttt gtacagtagg ttatgctatt gcttctgcta
    33121 atctaaaatt tatcataggc tcctcttacc tattcctcat caattgtagc tttattgtca
    33181 ttgcgactta tataggtgtt aggttgatga tggttaagaa acattatttt aaagataatg
    33241 aagaagactc taaaatgcgt aggattttgc ttctagttgc tgttttgctg atgattccga
    33301 gtttcatctc tgcaacgact ttagtgagag aaacgttgaa aaaagagtcc cttaagaaat
    33361 ttatatcaga gcagtttcag gggtataata ttttgaaaaa aacctattct aaaaagactc
    33421 ataccctaaa gctaaccatt tcaggaaatt atttgacaga agaagaactc gatatgattt
    33481 caagtaagag aggtgactat ggtttaagtg atgtttctgt tcaagtttca caattgtctg
    33541 attcagaaca acttagtaag gaagagctgg tggagcattt cttccagtat atcaaggata
    33601 aggaagcaaa agaaaaggag aaagccaata aatttgatac agagtctgag gagcaataat
    33661 ttcttgagaa tagctggttt ctctcgtgag tcttctatgt atatcaaagg aagactgagg
    33721 ttttaagtat aaaacttttc ttctattata gtagctaagt cttgacaggg ttcaaaaaat
    33781 ggtttacact ttataaagtt tattactttg aaaaggtgtg atactgtggc tacggcaaca
    33841 aaaaagaaaa aatcaacagt taaaaaaaat ctagtcatcg tggagtcgcc tgctaaggcc
    33901 aagacgattg aaaaatatct aggcagaaac tacaaggttt tagccagtgt cgggcatatc
    33961 cgtgatttga agaaatccag tatgtccgtc gatattgaaa ataattatga accgcaatat
    34021 atcaatatcc gaggaaaagg ccctcttatc aatgacttga aaaaagaagc taaaaaagct
    34081 aataaagttt ttctcgcgag tgacccggac cgtgaaggag aagcgatttc ttggcatttg
    34141 gcccatattc tcaacttgga tgaaaatgat gccaaccgtg tggtcttcaa tgaaatcacc
    34201 aaggatgcag tcaaaaatgc ttttaaagaa cctcgtaaga tcgatatgga cttggtcgat
    34261 gcccaacaag ctcgtcggat cttggatcgc ttggtagggt attcgatttc gcctattttg
    34321 tggaagaagg tcaagaaggg cttgtcagca ggtcgcgttc agtccattgc ccttaaactc
    34381 atcattgacc gtgaaaatga aatcaatgcc ttccagccag aagaatactg gacagttgat
    34441 gctgtcttta aaaagggaac caaacaattt catgcttcct tctatggagt agatggtaaa
    34501 aagatgaaac tgaccagcaa taacgaagtc aaggaagtct tgtctcgtct gactagtaaa
    34561 gacttttcag tagatcaggt ggataagaaa gagcgcaagc gcaatgctcc tttaccctat
    34621 accacttcat ctatgcagat ggatgctgcc aataaaatca atttccgtac tcgaaaaacc
    34681 atgatggttg cccaacagct ctatgaagga attaatatcg gttctggtgt tcaaggtttg
    34741 attacctata tgcgtaccga ttcgactcgt atcagtcctg tagcgcaaaa tgaggcggca
    34801 agcttcatta cggatcgttt tggtagtaag tattctaagc acggtagcaa ggtcaaaaac
    34861 gcatcaggtg ctcaggatgc ccatgaggct attcgtccgt caagtgtctt taatacacca
    34921 gaaagcatcg ctaagtatct ggacaaggat cagctcaagc tatataccct tatctggaat
    34981 cgttttgtgg ctagccagat gacagcggcc gtttttgata ccatggctgt taaattgtct
    35041 caaaaagggg ttcaatttgc tgccaatggt agtcaggtta agtttgatgg ttatcttgcc
    35101 atttataatg attctgacaa gaataagatg ttaccggaca tggttgttgg agatgtggtc
    35161 aaacaggtca atagcaaacc agagcaacat ttcacccaac cgcctgcccg ttattctgaa
    35221 gcaacactga ttaaaacctt agaggaaaat ggggttggac gtccatcaac ctacgcgcca
    35281 accattgaaa ccattcagaa acgttattat gttcgcctgg cagccaaacg ttttgaaccg
    35341 acagagttgg gagaaattgt caataagctc atcgttgaat atttcccaga tatcgtaaac
    35401 gtgaccttca cagctgaaat ggaaggtaaa ctggatgatg tcgaagttgg aaaagagcag
    35461 tggcgacggg tcattgatgc cttttacaaa ccattctcta aagaagttgc caaggctgaa
    35521 gaagaaatgg aaaaaatcca gattaaggat gaaccagctg gatttgactg tgaagtgtgt
    35581 ggcagtccaa tggtcattaa acttggtcgt tttggtaaat tctacgcttg tagcaatttc
    35641 ccagattgcc gtcataccca agcaatcgtg aaagagattg gtgttgagtg tccaagctgt
    35701 catcagggac aaattattga gcgaaaaacc aagcgtaatc gcctattcta tggttgcaat
    35761 cgctatccag aatgtgaatt tacctcttgg gacaagcctg ttggtcgtga ctgtccaaaa
    35821 tgtggcaact tcctcatgga gaaaaaagtc cgtggtggtg gcaagcaggt tgtttgtagc
    35881 aaaggcgact acgaggaaga aaagattaaa taagaggagg ggcccgaaag taaatttcag
    35941 gctccttttg gttagaactt gacaaaatac atcattttat gcgaaactag aagaagatta
    36001 ttttatactc aatgagaatc aaagagcaaa ttagaaagct agtcgcaggt tgctcaaagc
    36061 actactttga ggttgaagat ggaagctgac ctggtttgaa gagattttcg aagagtatta
    36121 actaggagaa gttatgcgta ttatctatct aattattggt tttttatcgc tgaccttggc
    36181 tattgttggg gttgttttac ccttgttgcc tacaacacct ttccttttgt tgtctattgc
    36241 ttgtttctcc agaagttcca agcgattcga agattggctt tatcatacca agctctatca
    36301 agcatatgta gctgattttc gtgagaccaa gtctattgcg cgtgaacgaa agaaaaaaat
    36361 catcgtctct atctacgtct tgatgggaat ttctatttat tttgcacctc ttttaccagt
    36421 caaaatcggt ctgggtgctt tgaccatctt tattacttat tatctcttca aggtcattcc
    36481 agacaaagaa tagttaaaac agtagttatt tgccttgata aaattgaaag catattcata
    36541 acaatatgat ataataaaat tgaagtaata ttcaaggaga atcaaatgat ttacgaattt
    36601 tgtgctgaaa atgtgacttt acttgaaaaa gcgatgcagg ctggagctcg tcggattgaa
    36661 ctctgtgata atctagcagt tggtgggaca acacccagct atggagtgac taaggcagcg
    36721 gttgaactgg cagctaacta cgatacaacc atcatgacca tgattcggcc acgtggtggt
    36781 gactttgtct ataatgacct agaaattgct atcatgctag aagacattcg tttgactgct
    36841 caggctggaa gtcaaggggt tgtatttgga gctttaactg ctgataaaaa gttggataag
    36901 cctaatctgg aaaagttaat tgctgcatca aaaggaatgg aaattgtctt tcacatggcc
    36961 tttgatgaac taagtgatga agatcaaccg gaagctattg actggctcag tcaagccggt
    37021 gtcactcgta tcctaactcg tgctggtgtg tctggcgact ccttagaaaa acgttttgtt
    37081 cactatcaca gaattttgga gtacgctaaa ggtaaaattg aaattctacc aggtgggggg
    37141 attgaccttg aaaaccgtca aacctttatc gaccaggtgg gggtaacaca attgcatggt
    37201 actaaggttg ttttttaaaa aatagaaagg aactgctagc tttgggtagc agttttcact
    37261 tatgtttgaa atttttaaat cctatcaatt taatcaagaa aaggctcatg attatggttt
    37321 tatagaaaat agcgaagtct ggacatatag ttgccagatt ttgcaaggtg actttgtcat
    37381 gactgtgtcc atcactgctg ataatgtgaa ctttcaagtc tttgaccaag agactggtga
    37441 cctctatcct cacgtttata tggaaagcat gaggggaagt tttgtcggaa atgtccgtga
    37501 ggcttgtctg gagattcttt accagattcg gaaggcttgt tttgatgtgc aagattttat
    37561 ctgtcatcag actaagcgta tcatgactca agttcaggaa aagtatggaa accagttgga
    37621 gtatctgtgg gaaaaatcgc ctgatacagc tgtattgcgc catgaaggca atcaaaagtg
    37681 gtatgccgtc ttgatgaaaa tctcttggaa taagctggaa aagggcagag aaggacaagt
    37741 ggaagcagtc aacctcaagc atgaccaagt agctaatttg ctttcacaaa aggggattta
    37801 tccagccttc catatgagca agcgctactg gattagtgtg tcccttgatg atactttatc
    37861 agatgaagaa gtactggaat tgatagaaaa aagttggaac ttaacctcta aaaaatgaaa
    37921 tattttaata attttcatga actttcaatt agctaaatat tctttactga agagattttt
    37981 agaaaatata ggatttacca cactagagga atatggtgcc atcttcaaat acctgattga
    38041 gaatgtcaag acggatcgtc agatcatcta ttcgcctcac tgtcatgatg acctcggaat
    38101 ggcagtggca aatagccttg ctgctgtcaa gaatggtgca ggacgtgttg aagggactat
    38161 caatggtatt agggagcgag ctgaaaatgc tgctttggaa gaaattgcag tggctctcaa
    38221 tattcgccaa gattactacc aagtagaaac cagtattgtc ctaaatgaga ccatcaatac
    38281 gtcagaaatg gtttctcgct tctctggtat tccagttcct aaaaacaaag ccgtcgttgg
    38341 tggcaatacc ttctcccacg aatctggtat tcaccaagat ggagtcctta aaaatcctct
    38401 cacttatgag atcatcacac ctgaattggt tggtgttaag attctgcttg gaaaattatc
    38461 tggtcgccat gcttttgttg agaaactgag agaattggcc ctagatttta cagaagagga
    38521 tatcaaacca ctctttgcta agttcaaggc actggtcgat aagaagcaag aaatcacaga
    38581 tgcagatatt cgagctttgg tagctggaac catggttgaa aatccagaag gcttccactt
    38641 tgatgattta caacttcaaa ctcatgcaga taatgacatt gaagcgctcg ttagcctagc
    38701 caatatggat ggtgagaaag tcgaatttaa tgcgacaggg caaggttccg ttgaagcaat
    38761 ctttaatgct atcgataagt tctttaacca atctgttcgt ttggtgtcct acactatcga
    38821 tgcggtaaca gatggaatcg atacccagga tcgggttttg gtcactgttg aaaacagaga
    38881 tacagaaacc atctttaatg cagcagggct tgattttgat gtgttgaagg cttctgctat
    38941 tgtctatata aacgctaata cctttgttca aaaagagaat gcaggtgaga tgggacgcag
    39001 tgtttcttac cacgatatgc ctagtgtgta aaggagaagg ctatggcaaa gaaaatagta
    39061 gctctagcag gagacggaat tggcccagaa atcatggagg ttggtttaga agttctggag
    39121 gctctagctg aaaaaacagg ttttgactat gagattgaca gacgaccgtt cggaggtgca
    39181 gatattgatg cagcatgacc tcccttacct gatgaaaccc ttaaggcaag tagggaagca
    39241 gatgctatcc tactagtagc tatcggtagt cctcagtatg atggagcagt ggttcgccct
    39301 gaacaaggcc tgatggctct ccgtaaggaa ctcaatcttt acgctaatat tcgtcctgta
    39361 aaaatctttg acagtctcaa gcatttgtca ctactcaaac tggaacgaat tgctggtgta
    39421 gactttgtcg tggtgcgtga attgacaggc gggatttact ttggagatca tattcttgaa
    39481 gagcgcaatg cgcgtgatat caacgactat agctatgagg aagtggagcg gattattcgc
    39541 aaagcctttg aaattgcaag aaatcgcaga aaaatcgtta ctagtatcga taagcaaaat
    39601 gttctagcga cctcaaaact ctggcggaaa gtagctgagg aagtcgcaca ggatttccca
    39661 gatgtaacct tggaacatca gctggtagac tcagctgcta tgcttatgat taccaatcct
    39721 gctaagtttg atgttattgt aacggagaat ctttttggag atattttatc tgatgaatca
    39781 agcgtcttat ctggtacact tggggttatg ccatcagcca gtcattctga aaatggacca
    39841 agtctctatg aacctattca cagttcagca cctgatattg caggtcaagg aattgccaat
    39901 cctatttcca tgattttatc agtttccatg atgttgagag atagtttcgg acgttatgag
    39961 gatgcagagc gtatcaaacg tgctgttgag acaagtctgg cggcaggaat tttaacgaga
    40021 gatataggag gtcaggcttc aacaaaggaa atgacggaag ctattattgc aaggttatga
    40081 agttagacga aaaaattact ctagtccttt tgatttggaa tgtcatcatt ttcttgattt
    40141 atggtattga caaatctaag gcaaggagaa gagtttggcg catccctgag aaaatcttac
    40201 ttattttagc ctttactttt ggtggttttg gtgcctggct agcaggaatc atctttcacc
    40261 acaagactcg aaaatggtac tttaaaatag tttggtttct tgggatggtg accacactag
    40321 tagccttata ttttatttgg aggtaatgga tggcagggtc ttcgagggaa tacgctgctt
    40381 gggctctagc ggactatggt tttaaggtcg tgattgcagg atctttcggt gacattcatt
    40441 acaataatga actcaataat ggcatgttgc caatcgttca gcctagagag gttagagaga
    40501 aactagccca gctaaaacca accgaccagg taactgtgga cttggaacaa caaaaaatca
    40561 tctcaccagt tgaagaattc accttcgaga tagatagcga gtggaaacat aaactcctaa
    40621 atagtttgga tgatatcggt attaccttgc agtatgaaga gttgattgct gcttatgaaa
    40681 aacaacgacc agcctactgg caggattaga aaaaatagaa aaggagatat agtaaactga
    40741 aataagatgt aaacaaatga attggagctt aacatccatt tccagcaatt ttttagaaac
    40801 tacagtggac tattctggat tcaacacatt ataaaattat gacaaaacac attcacaaga
    40861 aggctacgac attttaaaag gtgagggcgg atgtatcgtt tgccctacta aagttggtta
    40921 cattatcatg accagtgaca aggcaggact tgagcgtaag ttcgcagcca aagaacgtaa
    40981 gcgtaacaaa ccaggtgttg ttctctgcgg tagcatggat gaactttgcg ctttagcgca
    41041 actcaaccca gaaattgaag cattctacta aaaacattgg gatgaagata ttcttcttgg
    41101 ttgtatcctt ccttggaaac cagaagcctt tgaaaaactc aaagcatacg gggatggccg
    41161 tgaagaactt attactgatg tacgtggtac tagctgtttt gttatcaagt ttggaaaagc
    41221 aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg cctcatctgc
    41281 ttcaatgaca aaacgattga aactcgctat gagcaaggtg taatggtgtc tatggtcgat
    41341 aaggacggca aactcatccc agaacaagga ggagcacgtt caacttcacc agctccagtt
    41401 gtgatccgta aagggcttga cattgataaa atcatgatgc acctgtcaga tacttttaac
    41461 tcatgggact accgtcaggt tgagtattat taggatagag aagaagtcta gtgttatgag
    41521 atattaaagc tcctaacact gggcttttgt ttagaatttc ttttcttttt ctataggata
    41581 tggtattcta tgtagaaaat atatgttaat aagtaatgcc aatatttaaa catcattagt
    41641 aaaaggagtt agattgatga ataaaagaaa agttagttta gaagattttt ataaatggta
    41701 tagtctaaat aaagaagagt tattaaataa ggcaactgtt ggtgaaaagt ttaatgataa
    41761 attaaaagaa gagtttctcc aggaatggcc tttggatagg attttaacaa tgtcaatcga
    41821 tgaatatgta ataggaaagg gacagcaaaa taagtcttta tgctacgctc ttgagaaggg
    41881 aaaatacaaa aatctatttc ttggaatttc tggtggctca gcttcaaaat ttggtattta
    41941 ttggaataaa aaaacaaaca aatataaaga tcaagctaat aatgagattt cagagttgga
    42001 tcagcgattt tcaaaattaa aatcagattt gtatgaaatt atcaaagaag gtattcgttt
    42061 taactttgaa aatcctattt ttgatatgaa aagatcaaca aatgaattta ttggtcgttc
    42121 tgctatggtg acaaaattac tttgtatcta tactgaggga gatcctttct ttggtgtaaa
    42181 tattaatagt cagaaagaat tttggaacca ctttgtttct cagacaaatc aaggtggacc
    42241 ttatctgcaa aatcataaaa taattgaact ggtgtccaaa acttatcctg agttggagcc
    42301 atcgaaatta ggaactatgc tttttgagta ttctaagctt tttatggaaa ataaggaaga
    42361 caatagtaca atggattcat caaacaattt tagtcatcaa ttaactcaat ctctattaaa
    42421 gtctccaaac cttatccttc gtggtgctcc tggcacagga aagacttatc ttgctaaaga
    42481 aattgccaaa gaattaacgg atggtaacga agaccaaatc ggatttgtac aatttcaccc
    42541 atcctatgat tatacggatt ttgtagaagg tttaagacca gtatcaaatg gggatggagc
    42601 tattgagttt aggctacagg acggtatttt taaagatttt tgtcagaaag caaaagaaac
    42661 ccaattgatt ggaggacaag ataattttga tgaggcttgg gattcttact tagaatatat
    42721 aaatgttgct gaagaaaaag aatatataac aaaaacatct tacttatctg ttaatagtag
    42781 acaaaatttg tcagtaaatt atgatagtgg tgttccagga tggtcactac ctagcaaata
    42841 tgtttacgag ttgtataaag ataaaaatta taataagcaa gaatactaca aaagtggtgg
    42901 aaaaactgtc ctagaaacat tgagaaagag atttggtttg aaagactata tttctccaga
    42961 agaggttgaa acagacaaga aatttgtttt catcatcgat gagatcaatc gtggggagat
    43021 ttctaagatt tttggcgaac tctttttttc tatcgaccct ggctatcgtg gtgaaaaagg
    43081 aagtgtttct acccaatatg caaatctaca cgaaactgat gaaaagttct atatccccga
    43141 aaatgtttac atcatcggaa ctatgaatga tattgatcgt tcagtggata cctttgattt
    43201 tgctatgcgt cgtcgttttc gttttgttga agttactgcc gagggtcaag ttggcatgtt
    43261 ggataaagag ctgaatatcc atgcagaaga agcaaaaatt cgtctaagaa acttgaacgc
    43321 tgctatcgaa aatgttcagg aattaaacag tcattatcat attggaccaa gttattttct
    43381 taagttgaag gatgtagatt ttgactatga attactctgg tctgattata ttaagcctct
    43441 cctagaagac tacttgcgag gttcttatga tgaggttgaa actttggaaa ctttgaaaaa
    43501 agcatttgaa ctgacaaata atgagcaaaa agatcaggca gtagctgatg acaatgaagg
    43561 tgatgaaaac gatgatgcgg attactgata atcaacacaa gattattaaa gaaaaatttg
    43621 ttgaagaata tcctaaacta agcaatcttc ttttagacag aaccttggaa agtctatccc
    43681 aagatgaacg tattttcatt tttccaaatg atttgactca tactcctgat ttggataagg
    43741 atcaaaagat ttttgaaaca gtcaatcaga aaatcaagac agggaacgtg attggttttc
    43801 ttggatatgg tcaggaaaga ttaacgattt cctcacgatt ttctgatgag agtaatgacc
    43861 attttttgca ttatctctta aacaaggttc tttatatcaa tctcactagt ttagatgttg
    43921 ctttgtctcg tgaagagagg ctttatcaac ttttgatgta tctcttcccc aagtatctac
    43981 aagctgctat tcgaaaaggt ctttataagg aatatcatcg attttctcat aacgacagta
    44041 atgttaaggg agtgattgtt gtaagaaacc atctcaagaa aaatcttcct ttcacgggaa
    44101 atattgccta cacaacgaga gagttcacct atgataatcc cctcatgcag ttggtccgtc
    44161 acactattga atacattaag aatcagaaaa gcattggtca aggggtacta gataatctct
    44221 caactagtcg tgaaaacgta tctgaaatcg tgcgtgtaac gccctcttat aaactagctg
    44281 atcgtgctaa gattactcgg agaaatcaat ctaaacctat acgtcatgca tactttcacg
    44341 agtacagaaa cttacaagaa ctttgtctga tgatcctaaa ccaagaaaag cacggtttag
    44401 ggtatcaaga tcaaaaaatc tatggtattc tctttgatgt tgcctggctt tgggaagagt
    44461 atgtttacac cttgttgcca aaaggttttg tacatcccag aaataaggat aagacggatg
    44521 gaatttcagt attttctgtt gggaaacgaa aagtatatcc agatttttat gacagagaac
    44581 gaaagattgt tctagatgca aaatataaaa aactggaatt gactgaaaaa ggaatcaacc
    44641 gtgaggactt attccagctg atttcctatt cttatatttt aaaagctgag aaggctggac
    44701 ttgtttttcc gagcaaggac aaggtcattg ataatgagat aggaaaccta gcaggttatg
    44761 gagctttgtt gaagaaatgg tctatccaaa ttcctcagaa gtcttcatct tatagtgcat
    44821 tttgtaaaat gatggaaaat tcagaagaaa attttaaagc gattattgat gaagaagtgg
    44881 ggagaaaata atttcccctc tttcttttat ttttaacaca agcaaaatcc gaacaatata
    44941 ttttaattca taaaataatt tgacaaaaac tgtactttgg tttataataa attaaggaaa
    45001 cgtcggaaaa ggcgtagtga tgcgagagca taggtaggtc attacaaaag aaacgagaca
    45061 tcgatatgtt aaatgaattt ccaatttttg attacgaaga tattcaattg attccaaata
    45121 aatgtgtcat taaaagccgt gcagaagcgg atacaagtgt cactttagga aatcacacct
    45181 ttaaactacc tgttgtgcca gcgaatatgc agacgatttt ggatgaaaat gtagcagagc
    45241 aactggctaa aggtggttac ttctacatta tgcaccgttt tgatgaggca ggacgcattc
    45301 cttttattaa acgaatgcac gatcaagggc tcattgcttc catatctgtc ggtgttaagg
    45361 attatgagta tgattttgtt agccagctca aggctgatgc tcctgagtat atcactattg
    45421 atattgctca tggtcatgcg gatagcgtga tttctatgat tcaacacatc aagaaagaat
    45481 tgccagatac ctttgtcatt gctggaaatg tgggaacacc agaagctgtg cgtgaattgg
    45541 aaaatgctgg tgcagatgct actaaggtcg gaatcggtcc tggtaaggtt tgtatcacca
    45601 aggttaagac tggttttggt acaggtggtt ggcagttggc tgctctacgc tggtgtgcca
    45661 aggctgcgcg taaaccgatt atcgctgatg gaggaattcg tactcacggt gatattgcca
    45721 agtctatccg cttcggtgct agcatgatca tgattggttc cctctttgca ggacacattg
    45781 aaagtccagg gaaaacgatt gaagtcgatg gtgaacagtt caaagaatat tatggttcag
    45841 cctcacaata tcagaaaggt gcttacaaaa atgtggaagg caaacgtatc ttgcttcctg
    45901 ctaaaggtca tctgcaagac actttaactg agatggaaca agaccttcaa agtgctattt
    45961 cttatgcagg tggacgtcag gttgctgacc ttaaacacgt tgattatgtg atcgtgaaaa
    46021 actccatctg gaatggggat gcttcccact aagacggggt ctttgaccaa aaaacttgtt
    46081 attagagcaa atttctgtta taataaaaca agtttccacc cttagtgtaa tggatatcac
    46141 gtaagattcc ggttcttgag atgggggttc gattccctca gggtggatgt aaacatccta
    46201 aaaagccttt aatagggctt ttttctttat cctgacccaa atttgccgct aaaactgaga
    46261 atctctgtct aaagaattcc caaatgcttt ccagtcccgt tttagagtga ctcagggggc
    46321 tttttttgat ataataaaaa ggactattat cagttagaaa gaagttggta tgaaagaatt
    46381 acaaactgta ctaaagaatc attttgaaat cgaatttgca gacaaaaagt tactggaaac
    46441 tgcctttact catacgagtt atgccaatga gcaccgcctc ttaaaaattt cacacaatga
    46501 acgcttggaa tttttaggag acgctgttct acagttattg atttcagaat atctatataa
    46561 aaaatatcct aaaaagcctg aaggtgacct atcaaaactc cgtgctatga ttgtccgtga
    46621 ggagagttta gctggttttg cgcgtgattg ccagtttgac cagtttatca agttgggtaa
    46681 aggggaagaa aaatctggtg gtcgcaatcg tgacaccatt cttggtgatg cctttgaagc
    46741 ctttcttggt gcccttcttt tggataagga tgtggcaaag gtcaaggaat ttatctacca
    46801 agttatgatt cctaaggtag aagcaggcga atttgagatg attacagact ataaaaccca
    46861 tctccaagag ttgcttcagg tcaatggtga tgtggctatt cgttatcaag tgatttctga
    46921 aacagggcct gctcacgata aggtctttga tgtagaagtt cttgttgaag gtaagagcat
    46981 cggtcaaggc caaggtcgtt ctaagaaatt agcagagcag gaagctgcca aaaatgccgt
    47041 tgagaaaggg ctggattcat gtatttaaag gaaatcgaaa ttcaggggtt caagtctttt
    47101 gctgataaga ccaaggtcgt ttttgaccaa ggtgtgacgg cagttgttgg acccaatgga
    47161 tctggaaagt ccaatattac agaaagtctg cgttgggctt tgggggagtc tagtgtcaag
    47221 agtctccgtg ggggcaagat gccggatgtc atctttgctg gaacagaaag tcgcaaaccg
    47281 ctcaattatg cttctgtagt tgtgactctg gataatcatg acggatttat caaggatgca
    47341 ggtcaagaaa tcagggtgga acgccatatc tatcgtagtg gagatagcga atacaagatt
    47401 gacggcaaga aagtccgtct gcgtgatatt catgacctct tcttggatac tggattggga
    47461 cgagattcct tctctattat ttcccaaggg aaggttgagg agatttttaa ttccaagcct
    47521 gaggaacgac gagctatttt tgaagaagct gctggagttt taaaatacaa gactcgcaga
    47581 aaagaaaccg agagtaaact gcagcaaact caggataatc tggaccgctt agaggacatt
    47641 atctacgagt tggataatca aatcaagcct cttgagaagc aagctgagaa tgcccgtaag
    47701 tttttagact tggaaggaca acgtaaggct atttatttag acgttctggt tgctcaaatc
    47761 aaggaaaata aggcagaact agagtcgaca gaagaagagt tggctcaggt tcaagaactc
    47821 ttgatgagtt attaccaaaa gcgtgaaaaa ttagaagaag aaaatcaaac tcttaaaaag
    47881 caacgccaag atttacaggc tgaaatggcc aaagaccaag gcagtttgat ggacttgact
    47941 agtctgatta gtgatttaga aagaaaatta gccctatcga aactggagtc cgagcaagtg
    48001 gccctgaatc aacaggaggc acaagctcgt ttggctgctt tggaggataa gagaaattca
    48061 ctcagcaaag aaaagtatga taaagaaagc tctttagctc tgttagaggg aaatctagtc
    48121 caaaataatc aaaaactcaa tcgtttagaa gctgaattgc tggctttctc agacgatcct
    48181 gatcagatga ttgagctctt acgtgaacgc tttgtagctc ttttacaaga agaagcggat
    48241 gtctcaaacc agttgacccg tattgagaat gagttggaaa atagtcgtca gctttctcaa
    48301 aaacaagcag atcaactaga aaagctgaaa gagcaattag ctacagctaa agagaaggct
    48361 agtcagcaaa aagacgagct tgaaactgcc aaggtgcagg ttcagaaatt attggctgac
    48421 tatcaagcta ttgccaagga gcaagaggag cagaaaactt cctatcaagc tcaacaaagt
    48481 caactctttg accgtctgga tagtctcaaa aacaagcagg ccagagctca aagtttggaa
    48541 aatatcctga gaaatcatag taacttttat gcaggtgtta agagtgttct ccaagaaaaa
    48601 gatcgcctag gtgggattat tggtgcagtc agtgagcatc tgacctttga tgtttattat
    48661 caaactgccc tagagattgc cttaggggca agtagccagc atatcatcgt agaagatgaa
    48721 gagtcggcaa ccaaagctat tgatttcctc aaacgaaaca gagtcggtcg tgcaaccttt
    48781 cttcctttga ccactattaa ggcgcgtacg atttctagtc agaaccaaga tgctatcgct
    48841 gtaagcccag gtttccttgg gatggcagat gagttggtga cttttgatac tagactggaa
    48901 gccattttca agaacttgct agctacgacg gctatctttg ataccgtaga acatgcgcgt
    48961 gaagctgctc gacaagttcg ttatcaggtt cgtatggtga cattggatgg gacagaatta
    49021 cgcacgggtg gttcctatgc gggtggtgcc aatcgccaaa ataacagtat tttcatcaag
    49081 ccagaactgg agcaattaca aaaagaaatt gctgcagatg aagcaagctt gggttcagaa
    49141 gaagcggctt tgaagacctt gcaagaccag atggctgcat tgacagaaag attagaagcc
    49201 atcaaatctc aaggagagca ggcacgtatt caggagcaag gcttgtccct cgcttatcag
    49261 caaactagtc agcaagttga agaactggaa actctttgga aactccaaga agaggaaata
    49321 gatcgtcttt ctgagggaga ttggcaagcg gataaggaaa aatgtcaaga gagccttgct
    49381 actatcgcca gtgaaaagca aaatctggaa gctgagattg aagaaattaa gtctaataaa
    49441 aacgccatcc aagaacgcta tcaaaatttg caggaagagg tggcgcaagc tcgcttgctt
    49501 aagacagaac tgcaagggca aaaacgttat gaagtagctg atattgagcg tttaggcaag
    49561 gaattggaca atcttaatat cgaacaagaa gaaattcagc gcatgctcca agaaaaagtt
    49621 gacaatcttg agaaggttga tacagaattg ctcagtcaac aggcggaaga atccaaaact
    49681 cagaaaacaa atctccaaca aggtttgatt cgcaagcagt ttgagttgga tgatatagaa
    49741 ggtcaactgg atgatattgc cagtcacttg gatcaagctc gccagcagaa tgaggagtgg
    49801 attcgcaagc aaacacgtgc tgaagccaag aaagaaaagg tcagcgagcg cttgcgccat
    49861 ctacaaaatc aattaacaga ccagtaccag attagctata ctgaagcact agaaaaggca
    49921 catgaattgg aaaacctcaa tctggcagag caagaggtgc aggatttaga gaaggctatt
    49981 cgctcattgg gacctgtcaa cttggaagct attgaccagt acgaagaagt ttacaaccgt
    50041 ctggactttc taaatagtca gcgagatgat attttgtcag cgaaaaatct gctccttgaa
    50101 accattacag agatgaatga tgaggtcaag gaacgcttta aatcaacctt tgaagctatt
    50161 cgtgagtcct ttaaagtgac cttcaagcag atgtttggcg gaggtcaggc agacttgata
    50221 ttgactgagg gcgacctttt aacagctggt gtggagattt ctgttcaacc tccaggtaag
    50281 aaaatccagt cgcttaacct catgagtggt ggtgaaaaag ctctatcggc tcttgccttg
    50341 cttttctcca ttattcgtgt caagaccatt ccttttgtca tcttggatga ggtggaagct
    50401 gcgctggatg aagccaatgt taaacgtttt ggggattacc tcaaccgctt tgacaaggac
    50461 agccagttta tcgttgtaac ccaccgtaag ggaaccatgg cagcggctga ttccatctat
    50521 ggagtgacca tgcaagaatc gggtgtttca aagattgttt cagttaagtt aaaagattta
    50581 gaaagtattg aaggatgaca attaaactag tagcaacgga tatggacgga accttcctag
    50641 atgggaatgg acgctttgat atggatcgtc tcaagtctct cttggcttcc tacaaggaaa
    50701 aagggattta ctttgcggtg gcttcgggtc ggggatttct gtctctggaa aaattatttg
    50761 ctgatgttcg tgatgacatt attttcatcg cggaaaatgg cagtttggta gagtatcaag
    50821 gtcaggactt gtatgaagcg actatgtctc gtgactttta tctggcaact tttgaaaagc
    50881 tgaaaacgtc accttatgta gatatcaata aactgctctt gacgggtaag aagggttcat
    50941 atgttctaga tacggttgat gagacctatt tgaaagtgag tcagcattat aatgaaaata
    51001 tccaaaaagt agcgagtttg gaagatatca cagatgacat tttcaaattt acaaccaact
    51061 tcacagaaga aacgctagaa gctggtgaag cttgggttaa tgaacatgtc cctggtgtta
    51121 aggccatgac aactggcttt gaatctattg atattgttct ggactatgtc gataagggtg
    51181 tagctattgt tgaattagct aaaaaacttg gtatcacaat ggatcaggtc atggcttttg
    51241 gagacaatct taatgactta catatgatgc aggttgtggg acatcctgta gctcctgaaa
    51301 atgcacgacc tgaaatttta gaattagcaa agactgtgat tggtcaccat aaggaacggt
    51361 cggttatagc ttatatggag ggcttataat ggcagatata aaattgattg cattggactt
    51421 ggacgggacc ttgctgacta ctgataaaag gctgacggat cgtaccaagg aaaccttgaa
    51481 agctgcgcgt gatcgtggta tcaaggtcgt attgacaact ggtcgtccct taaaagccat
    51541 ggatttcttt ctccatgagt tagggactga cggtcaggaa gatgagtata ccattacttt
    51601 taatggtgga ttagttcaga aaaatacagg agaaatcctt gataaaacag tcttttcata
    51661 tgatgatgtg gcacgtttgt atgaagaaac agagaaatta tcactgcctc ttgatgccat
    51721 ctcagaagga acagtttatc aaatccaatc ggaccaagaa agtctttatg ccaaattcaa
    51781 tccagctttg acctttgttc cagtggactt tgaagactta tctagtcaaa tgacctacaa
    51841 caaatgcgtg actgcctttg ctcaagaacc cttggatgca gccattcaga agatttctcc
    51901 agaattgttt gaccaatatg aaatctttaa atcacgtgaa atgttgctag aatggtcacc
    51961 aaagaatgtt cataaagcaa caggtttggc aaaactaatc agccatcttg gaatcgacca
    52021 aagtcaagtg atggcttgtg gtgacgaggc caatgacctc tctatgattg aatgggcagg
    52081 tcttggtgtt gctatgcaaa acgctgttcc tgaagtaaag gcagccgcaa atgtagtgac
    52141 gccgatgacc aacgatgagg aagctgtcgc ctgggctatc gaagaatatg tgctaaagga
    52201 gaactaagat atgggattgt ttgaccgtct attcggaaaa aaagaagaac ctaaaatcga
    52261 agaagttgta aaagaagctc tggaaaatct tgatttgtct gaagatgttg atcctacctt
    52321 cacagaagtt gaggaagttt ctcaggaaga agcagaggtt gaaattgttg aacaagctgt
    52381 gttccaagaa gaggaaatcc aagacacagt tgaagaaagt ctggatttag agccagttgt
    52441 agaagtttct caaaaagaag tcgaagaatt tccacactca gaagaaggga atactgagtt
    52501 tctagagact atagaagaaa ataattctga agttcttgaa ccagaaaggc ctcaagcaga
    52561 agaaaccgtt caggaaaaat atgaccgcag tcttaagaaa actcgtacag gtttcggtgc
    52621 ccgcttgaat gccttctttg ctaacttccg ctctgttgac gaagaatttt tcgaggaact
    52681 ggaagaactg ctgattatga gtgatgttgg tgtccaagtc gcttctaact taacggagga
    52741 actacgttac gaagccaagc ttgaaaatgc caagaaacct gatgcacttc gtcgtgtcat
    52801 cattgagaaa ttggttgagc tttatgaaaa ggatggtagc tacgatgaaa gcatccactt
    52861 ccaagataac ttgacagtta tgctctttgt tggtgtgaat ggtgttggga aaacaacttc
    52921 tatcggaaaa ctagcccacc gctacaaaca agctggtaag aaggtcatgc tggttgcagc
    52981 agataccttc cgtgcgggtg cagtagctca gctagctgaa tggggccgac gagtagatgt
    53041 tccagtagta actggacctg aaaaagctga tccagccagc gtggtctttg atggtatgga
    53101 acgtgccgtg gctgaaggta tcgatattct catgattgat actgctggtc gtctgcaaaa
    53161 taaggataac cttatggctg agttggaaaa gattggtcgt attatcaaac gtgttgtgcc
    53221 agaagcacca catgaaacct tcttggcact tgatgcatca acaggtcaaa atgccctagt
    53281 acaggccaaa gaattttcga aaatcacacc tttaacggga attgttttga ctaagattga
    53341 tggaactgct cgaggaggtg tggttctagc cattcgtgaa gaactcaata ttcctgtaaa
    53401 attgattggt tttggtgaaa aaatcgatga tattggagag tttaactcag aaaactttat
    53461 gaaaggtctc ttggaaggtt taatctaatc agaagcaaaa atcctgcaag gcataaactt
    53521 gcaggaaatt tttttattct aagcgaccat cttgacgata ggtgatatct ggttgccaag
    53581 tccatttggc accgaatttt tcaagtaggt caaagctggc ttgaggtccc atgcttccag
    53641 ctttatagtc atgaagtggg gcaccatttt cagcccagag cttttcaata cggtcaatca
    53701 acttccatga cgcacaaact tcatcccagt ggctaaagtt agttgagttg ttatttagga
    53761 catcataaat caatttttcg tatggttctg gagaagcacc agttgcagtc gcatctgtac
    53821 ggtaatcaag tgagttagga gccaagttaa attcttctcc tacttgcttc ccatttaggc
    53881 taagagagaa gccttctgtt ggttgaatat agatggtcaa aatatttgga gcaagtggtt
    53941 ctccaaagat agaatccatt tgtttaaaga cgatgttgac atgagttcct ttttcagtca
    54001 gtcgtttacc tgtacggaaa aagaaaggaa caccacggaa tcgatcgctg tctacaaaga
    54061 aggcaccaga tgtaaaggtt tcagttgttg attctggatt cacatttggc tcgctacgat
    54121 aagagatgta tttcatgcca tcaatcttac cagagcggta ttgcccacgg ataaagtgtt
    54181 ctttgagttc ttcatcagtt ggatgataga ggtttttaaa gaccttaatc ttttcagcac
    54241 gaatctcgtc ttttgtgaag cttgctggtt tgtccatggc gaggagcgaa agaagttgta
    54301 gagtgtggtt ttggaccatg tcacggaggg caccggattg gtcatagtag ccaccacgtt
    54361 cttctacacc caagcgctcc gcaaaggtaa tttgaacatt gtcgataaaa tccttgttcc
    54421 aaacgttttc aaaaatcaag tttgcaaagc gaactgcaaa gatgctttgg atcatttcct
    54481 taccaagata atggtcgata cggaaaattt gttcttcgtc aaatgttgct aggagttcgt
    54541 cattcaactt gtttgcagtt gcgtaatctg taccaaatgg tttttcaacg atcaagcgct
    54601 caaaaccttt gccatcgaca atgttttcag atttgaggtg tttggcaatg gttccaaaga
    54661 actgaggtgc catagacaag aagaagagct tattgtgttc agcttggtat ttttcattaa
    54721 gctcagcttg taattgacgc aaagcaatat aatgttccga atcattgaca tcatggcttt
    54781 gatagtagaa gtggctagca aattcttggg cttgctcggt actatctgcc aaatcaagga
    54841 tggactcgac aactacagat tcaaaatatt ccttactcca aggtctacgg gcagttccaa
    54901 taacggcaaa gtgcttggaa agattgccgg atttatatag tctaaaaagg gaagggtaga
    54961 gcttgcgttt agccaggtct ccactcgcac cgaaaattgt aacaataacc ttagatgaca
    55021 tctagctacc taatttctat ttttattcct agtcttgcta ggtatgagga aacctcattt
    55081 cataaactta attctaacat aattttccga aaaatcaaaa attccaatac gaggtagaaa
    55141 aactgcaagg aaatccttac agtttgagtt tagacgttta agaccttatc taagaactct
    55201 ttcagacgag ggtgttgtgg gttatcaaag atttggtcag gtgttccgtc ttcaaggaac
    55261 tcgccatctg cagtaaagat aacgcggttg gcaacctgac gagcaaatcc catctcatgg
    55321 gttacgataa tcatggtcat gccttgctca gccaattcct tcataacgtt aagtacgtct
    55381 ccaaccatct cagggtcaag ggcagaagtt ggttcatcga agagcatgat gtctggattc
    55441 attgctaggc cacgagctat ggccacacgt tgtttttgac cacctgatag gctatctgga
    55501 ttggcattag ctttatctgc tagtccaacc ttttcaagca actccattcc caatttctca
    55561 gcttcttcct tagtcatcaa cttgtgctca ataggagcaa aggtgatgtt gtccaataca
    55621 gacatatgag ggaagaggtt gaagtgttgg aataccatgc cgatattttc acggacgtgg
    55681 tcaacattgg ttgttttttc agttaaatca tagccgttca cagtgatgtg accgctagtg
    55741 acttcttcta aaagattgag gctacggagg aaagttgact taccagaacc tgaaggaccg
    55801 atgatacaaa caacatctcc ttcatagaac ttagtcgtaa ttccttttag gacttcattt
    55861 tttccatagt gcttgtgtaa atcatttaca tcaattttta attttgccat taacgaatcc
    55921 tcttttctaa gcgtttcgct agtctagtca aaagtgtgat aattacaaga tagaagatag
    55981 caaggattgc atacatcttg aaactttggt agttacgagc aatgataatc ttaccagttt
    56041 ggaagagttc aaccaaaccg atagcagata cgatagttgt atctttaaga gcgataacga
    56101 attggttgac aaagtttggc aacatcaatt tagttgcttg tggcaagata atcttacgca
    56161 tggtttttcc ataagagata cccaagcttc ggctggcttc catttggcca actggaacgg
    56221 cctgaatacc accacgaacg atttcagcga tataagcagc cgcattgagt gagagggcaa
    56281 tggttccagc tacaaagtcg ttaattgggc tttgttggcc tgtgatagac tcgatgaagt
    56341 ttggaattcc ccagaagatg aaggctgcaa gaatcatcaa tggaatacca cgaataacgt
    56401 caacgaaaat ctcagagatg acgcgaagag atttgtatgg gctaacgcta aacataccga
    56461 agataattcc gatgacaatg gcaatagcaa atgagataag agctagagca agagtgatac
    56521 caagaccgct aaggagttgt ttgtagttgt tttgaagcaa gccccagagc gttgtttcgt
    56581 caacagtact tgttgaagca gttgaagatt cgctagctag gtatttgtca agaatctttt
    56641 ggaattcacc gtttgcttta aggtttgcaa gtccgttgtt gaacatttca atcagttctg
    56701 gatttgctcc ttttttaacg gcaaaggctg tttcaccgat tggagttcca gagattggag
    56761 ttttcaattt ttgaccttgg ctgatagaat atttgagaac aggttcatca tccataacgg
    56821 catcaatggc accagtgttt aaactgtcat acattgaaga accatcagca aaggttttga
    56881 ttttgtagcc gtatttgctt tgattttctg ttaggaaggt ttgagaagca gttccgtttt
    56941 taacaccgac tgtctttcct tttagatctt cataagaagc aatattactt gattctttga
    57001 caccaagaat ggtattagca gtgtagtatg attctgagaa gtcaaaagtt gccttacgag
    57061 catctgtgac agacatacca gcgatgatac catcggcttg accagcttgg acagcactga
    57121 tagcagcatc aaaaccaggg ttggtgattt caatttcaaa accttggtct ttagcgattg
    57181 ccttaatcaa ttccatatca ataccagtgt attgattgct tgaattttgg aaaacaaaag
    57241 gtgcaaaaga agaatcgctg gcaatgatat atttagcctt aacaggaata gcttttaatc
    57301 ctgcgagagt agttgtagtt ggcactgctg aagatgatga agcagtccat ttcttgataa
    57361 ttttatcaag actaccatct tttttcattt cagccaaggc ttggttaaat tcagtaacca
    57421 ggtgctcgta tttacttcct tttttcacac cgaaagcaaa acttcctaca gcttcaccat
    57481 ccatttcaat atggaggtct tgaccttggt taatggcata ttcgataaca ggtttgtcat
    57541 ccatcatggc atcgatggca ccagcactca agctgttgtt cattaaatca ccagtgtcaa
    57601 atgttttaat agtaaagccg tatttatctt tgattgtttc aaggaaacgt tgagcggcag
    57661 ttccgttttt aacaccaacg gttttgccag ttaattggtc gtacttgcta attttgtgtg
    57721 actttgtagt agcaatgaca acttttgtat catagtaagt atcagacatg gtgaagactt
    57781 tttcacgttc tttagtcttt gtcatccctg ccatgatagc gtcggcttgc ccagcttgaa
    57841 ccgcattgac tgctgcgtca aatccaggat aggacatctg aatgttccag cctttaatct
    57901 cagcgacttt gttaataatg tcaacatcaa ttcctttata agtttgatct gaatctttaa
    57961 actcaaaagg tgcataggcg gtatcagaaa caatcttaat cgtttctgct ttggcaatac
    58021 ctaatgagaa aattgggaat aaaattagca aaaatgctag aaattttttc ttcattttta
    58081 gtctcctttt ccgaatattc tctcattata tcagaaaaag taaaaaaagg caaattttta
    58141 tatttttcgt gtcagaaaat ataacattga ttttttcctt ttcttgaatt gctattaaaa
    58201 agtgctataa taatagtata tatagaagaa aagaggacgg ggatatgaag aacaaaagaa
    58261 tatttaaaga cttccaagct tcaaaaatga gtttaaacat ttacacaagc cccttgttag
    58321 ccattgtttt tgtcttcata ggagagtttg tggcttttac tttgtatggt attggcttgt
    58381 tagctctcat cggacttgct agaaattttg gagaggctgg tcaaaatctt gcaacctact
    58441 tgcagacctt gcatcagagc ttgacggata aaacaagtga ctttcgttta attttaggat
    58501 tactggcctt tggttttatt cttaacactg tgttcagatg gacaagaaaa gttgagaaaa
    58561 gacctattcg aaccttggga ttttatagag agaattgcct cagcaatctt ctgaaaggat
    58621 ttagtctagg tctggcactt tttcttctga ccttgttagg tttggtggtc ttaggtcaat
    58681 atcgtttgga atccattcac ttgaatcctt attctcttgc ctttgtcgtc tttactatcc
    58741 cattttggat tttacagggg acagcagaag aagtggtggc ccgtgcttgg ctacttcctc
    58801 aattggcctc aagaaccaat ctaaaactag ctattcttat atctagcctg ttctttaccc
    58861 tgcttcatat gggcaattct ggcctcaccc ctctatctct agtaaatctc tttttattcg
    58921 gagttgccat ggctctttac cttctcaaaa ctgatacagt ttggggtgtt gcaggtattc
    58981 atggtgcttg gaattttgct cagggtaatc tctttgggat tttagttagt ggtcaaccgt
    59041 caggaacgtc tctgatgacc tttttaccac aaggcaatca agattggtta tcaggtggtt
    59101 cttttggcat agaaggttcc attatgacaa gtctggtatt actactgctg attgtctatc
    59161 ttgctaataa attaaagaaa gaaaatgaaa ggatgtgact tcggtccgtc cttttcttcg
    59221 tgaaaatact ataagtatgc taaaatagga atagcacatg gagagaggat tcttatgatc
    59281 aatcacatta cagataatca atttaaacta gtatcaaaat atcaaccatc aggagatcaa
    59341 ccccaagcta tcgagcagtt ggtggataac attgaggggg gagaaaaagc tcagattctg
    59401 atgggggcga ctggaacagg gaagacctat actatgagtc aggtcatttc taaagtcaat
    59461 aaaccaactc tggttattgc ccacaataaa actctggctg gtcagctcta tggggagttt
    59521 aaggaatttt tccctgaaaa tgcagttgag tatttcgtat cctactatga ttattaccag
    59581 ccagaggcct atgtcccttc tagcgatacc tatattgaga aggatagttc tgtcaatgac
    59641 gagattgaca agcttcgcca ctcagctacc tcagcccttt tggagcgtaa tgatgttatt
    59701 gtcgtggcct cagtctcttg tatctatggt ttgggttcgc ccaaggaata cgctgatagt
    59761 gtcgttagtc tccgtcctgg tctagagatt tctcgtgata aactcttgaa tgacttggtc
    59821 gatattcagt ttgaacgcaa tgatattgat ttccaacgcg gaagatttcg cgttcgtggg
    59881 gatgtggtag agattttccc agcttcccga gatgaacatg cctttcgagt agaatttttt
    59941 ggagacgaaa ttgaccgtat tcgtgaagtt gaggctctga caggtcaggt gttgggagaa
    60001 gtggatcatt tagcgatttt cccagcgaca cactttgtga ccaatgacga ccacatggaa
    60061 gttgccattg caaagattca ggccgagttg gaagaacaat tagctgtctt tgaaaaggaa
    60121 ggtaaactgc ttgaagccca gcgtttgaaa cagcggacag agtatgatat cgaaatgttg
    60181 cgtgagatgg gctataccaa tggggttgaa aattattctc gccacatgga tggacggagc
    60241 gaaggagagc ctccttatac gcttctcgac ttcttcccag atgatttctt gattatgatt
    60301 gacgagagtc atatgaccat agggcaaatc aagggcatgt acaatggaga ccgttcgcgt
    60361 aaagaaatgc tggttaatta tggtttccgt ttgccgtctg ctttggacaa tcgtcctctc
    60421 cgtcgggagg agtttgagag tcacgttcat cagattgttt acgtttcagc gacacctggt
    60481 gactatgaaa atgaacagac cgagacagtg attgagcaaa tcattcgtcc aacgggactc
    60541 ttggatccag aggtggaagt ccgtccgact atgggacaga ttgatgacct cttgggtgaa
    60601 atcaatgccc gcgttgaaaa aaatgagcgt acctttatca caactttgac caagaaaatg
    60661 gcagaggact tgaccgacta cttcaaggaa atgggtatca aggttaagta catgcactcg
    60721 gatatcaaga ccttggaacg gacggagatt atccgtgacc tgcgcttggg tgtctttgat
    60781 gtcttggtcg gaattaacct gctccgtgaa ggaattgacg ttcctgaagt gagcctcgta
    60841 gctattctcg atgctgacaa ggaaggtttc cttcgcaacg aacgtggact catccagact
    60901 attggacgtg ctgctcgtaa cagtgaaggc catgtcatca tgtatgcaga cacggttacc
    60961 cagtctatgc aacgtgctat cgatgaaact gcccgccgtc ggaaaattca gatggcctat
    61021 aatgaagagc atggtatcgt accacagaca atcaagaaag aaatccgtga cctgattgct
    61081 gtgaccaagg cagttgctaa ggaagaggac aaggaagtcg acatcaatag cctcaacaaa
    61141 caagagcgca aagaactcgt caagaaacta gaaaaacaaa tgcaagaagc cgtcgaagta
    61201 cttgactttg aactggcggc tcagatccgt gatatgatgt tggaagtcaa ggccttggat
    61261 taggggaata gtatgattta tttaagaaag ttaaagagag aagatttgat gtctttatgg
    61321 gaaatggctt attcacaact taatccagtt tggaaacagt atgatgctcc ctattatgat
    61381 gattatcagt atttttcaaa ttttaaagaa ttcgaactac aaaaatcaga atccatttta
    61441 agcaactcaa atcgccttgg tatttttgtt gatgataaac tagttgggac tgtttcgcgt
    61501 tattgggtat gtaaacaaac aagatggatg gaattgggaa ttggtattta tgataaaaaa
    61561 ttctggaaca ctggtattgg gaaagttgct atgttgcagt ggatagatag gacgtttcag
    61621 gattacttgg agttggagca tctgggtttg acaacttggt cagggaatct tggtatgatg
    61681 aaacttgctg aaaaattaag aatgaaaaaa gaagctcata ttccaaaagt tcgttattat
    61741 caaggtaaat attttgatag tattaaatat ggtattttga gagaagactg ggagaaaaca
    61801 aatgacggtt attatcaaat caatggaaac tcctgaagag atagaaggta agccccttgt
    61861 tcactggcaa acgtggagag aggcttatga tggccttttg cctgcagaat ttcaggagac
    61921 aatgacatta gaaagatgtc gattctttag tcaaaagtat ccagaaaata cattgattgc
    61981 gatggatggt atgaaggtag ttggttttat cagttatggc aactttcgtg atgagactat
    62041 tcaagctggc gaaattattg ctttatatgt tttaaaagac tattatggaa aaggtatcgc
    62101 acaaaagtta ataaaagcag ctttgattgc tcttaatcat ttttctgaaa ttttcttatg
    62161 ggtattgaaa gataataagc gcgcaattgc tttctatcaa aaaatgggct ttacttttga
    62221 tgaacaagaa aagatacttg aacttggaaa gcccataaag gaaaaacgga tggtattcta
    62281 ttctaaataa ttttaaaaag tagaagctaa tggtactggt atcaagtcta aaaatttaat
    62341 aaattagaaa gtgagtaaat ttatgtctcg agcacaatta acgattttaa caaatatttg
    62401 tctgattgaa gatctcgaag ctcagcgcgt ggtgatgcag tatcgcgctc ctgaaactaa
    62461 tcgctggtct ggttatgcct ttccaggagg ccatgtagaa aatggcgaat ctttcgcaga
    62521 gtctgtcatt cgtgaaatct atgaagaaac agggttgact atccaaaatc ctcaacttgt
    62581 cggcattaaa aattggccac tagatacagg tgggcgctat attgtcgttt gttataaggc
    62641 aactgagttc tctggtaccc ttcgctcttc agatgaagga gaagtttctt gggtgcaaaa
    62701 agaccagatt ccaaacttgg atctggccta tgatatgcta cctttgatgg agatgatgga
    62761 agcatcggac aaatctgaat ttttctaccc tcgccgtaca gaagacgatt gggaaaagaa
    62821 aattttctaa tcctttacta aataacctag ctgatccaag gcctcctcga tatagtggag
    62881 gtcttgttgt gtttcggctt cgactaggtg gtaatggaca ccatctgtca attcagataa
    62941 aggtttaaaa tcagaatgct cgacttgttc tagaaaatgt tgaacgtctc ggcggcaaga
    63001 cagtttaagc aaggtttcaa tttctccata aacgggatgg tcaatcaaag tattctggac
    63061 atgcccacca ttatcgacaa tagcaaggag ttcctgaccg atttcttcaa cttcatgttt
    63121 cactttgaaa agtttgtgaa cataaggatt ggtatcaatt tgcttataga tgtagccacg
    63181 attggtggat aggataggag cgccatcggc tctcaaaatt gcaatgtcct gcacaatgac
    63241 ctgacgtgtg acatgaaaat gttccgccaa actttggcca ttaagggctt taggagcttc
    63301 tttcaagagt tggagcaggg cttgtttgcg atcttttgtc atagcttttc cttttaacgg
    63361 cgttttcgaa gcactttata gacagctagt gctaatgtat agtctaccat actatggata
    63421 attgtgccaa atccaactag tacaaataga acatagaaca tgttttctac attggtaccc
    63481 gaagttgcgt aaaaaaggac acaggctaat acttcagcaa gggcatgaac aacagccaaa
    63541 acaaaattga aaacccagga agattttggt ttatctaggg tatcggggaa tttttgtagg
    63601 taaagagctc ctaaagcacc aaaagatata tgggaaaaag cccgaaaaac tataaccatg
    63661 ggatagccag ccatcaaaaa tccaaaacta gaggctagga tgacaaaaac tgccatcaag
    63721 ggcgacaaga acatggctat aaaaatagcg atgtggctcc ccaaagtata ggaagcaggt
    63781 ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa tcgcaatagc cgttaaaagg
    63841 gctgtcattg tcataaattg tgtctttttc cgtgtattca caagaatctc ctttttaact
    63901 gcatatacac tagtatggta caataaacca gacaataaag caagaattta cttgggttta
    63961 tagatcattt tttagttaaa agttatagta gattgaaact agaatagtcc acctctactt
    64021 ctaaaacatt gttagaaatc tatttgactg tcctgatcga tttgtcctgt tcttatttcg
    64081 ttttactata gtaaagattt cattaaaaag aaactgtata gagcaaaatc tccaccttca
    64141 ggtttgaaag cggagattgt ttttattttt tcaaggtttg tagtcgtgga acaatggcta
    64201 gggttagaat tgcagaaatg accaattcag caattgaatt tgttgagata acggttgcca
    64261 gaagaagttg gatatttcca ttataaacat ttccaaatag gaagaagatt cctccaagga
    64321 caaagatagt atttgtcaag gaaccaaggg ctccagctaa aatcagacca gtcttgtttt
    64381 tcatcagttt atagactaag taaggagtta aaccaatcaa aatacgtggg acgatggcaa
    64441 tgatagctga gtagatgttt ccgtttggta cgaagggaga gaagaggtag cttgtcggta
    64501 gaatcgtaat cgtgttaacc gtcaagctaa gtaatcccat caaaaatcca agtgtaatcc
    64561 caactcgtgg accataaata atgctggcaa taatgacagg aatatgaaca atggtcggtt
    64621 tgattggaaa tggaaaaagg ttaaagataa gtgagctcag aaagtgtatc acgagcatgg
    64681 tagcaaaaaa gatagcaatg ggtgcaatat tagagcgttt tttcatcgat agtttccttt
    64741 attctttcta aaataattgt gaggtcagct aaagctcctc gtccgtggtc tccacaagct
    64801 agtagggatt ccttaggagc aatcagctga tagccgtagg tttctaatgt tttcagatta
    64861 ttctgagtta ctggatggtc atacattttt gtattcatag caggagctat tagtttggga
    64921 atatgacttg gtagggctag agctgtactg gttaccatgt tgtccgcaaa tccgtgagct
    64981 agttttgcaa tagtgttagc agttgcaggt accacgataa ataaatctgc tttttttcca
    65041 agttcgatat gattgacctg atcaggatag ggttctttca tgacatccaa gtggacagga
    65101 ttctgtgaga gtacctgtag tgtcaaaggt tggataaact ctgtagcagc ctgagtcatt
    65161 aagacagtga cttgatggcc ttgttttttt agagaactga ccaaatctgc cgacttataa
    65221 gaggcgattg aacccgttac agccaagaga atgtttgcca tagttttcct ttctaagaat
    65281 gataggcttg aattttttca aggaggagtt ctgcaatttc ttctttagtc tggactgttt
    65341 gaagctgatt tttctcaaca aatatagctc ggtgctgacc tgctgaaatt tgagtcaggt
    65401 cattcgcgat gattaaatct gcttgattct tgataagact ttttcgtgca atgtcaacca
    65461 gatgatcttc ggtaacatca accagcagtt tgaaaccaat cagatgaata gtaggattcc
    65521 attccttgac tagggatatg attttgggtg tctttttaag gaacaaaacc tgaacctcat
    65581 cagttgaaga aatcttggcc tgatgattct gcttgcttaa aaattctttt agattggagc
    65641 tagcctgaac ttcctcaagc cctgtcatat aaacaggagt gtagtcagaa acagccattg
    65701 agtggatcaa gacctgataa tcctgaacac gttcttgcat ttctattaga aggtccttgg
    65761 tattggtaat ttctcgaata cttaggttag gatgaggctc tggcttcaga gctcgttttg
    65821 tcgtaattaa acaaacttca tgccctgcag aaagcaaagt ctctgtgata attttcccca
    65881 agtgacctgt agaatggtta gtgatagagc ggacgctatc gatagcttca ctggtaccgc
    65941 ccgatgtaac taaaattttc atagcactat tgtacacaaa aataagcggt aagtaaaatg
    66001 aaagtgataa atttttggta aaaacgaaga tttactatag tttcaaacta taaagccata
    66061 taaaatttaa gaaaggactc taaaagcctt aaaaacagag atatttacga acgtttagag
    66121 agttttagga tgttaaaaat cttgttttgt gatataatga attatcatat aagaggttag
    66181 aggaattttg aatgaaaaca gatattgaaa tcgcacagag tattgagttg aagccaattg
    66241 ttgatgttgt agagaaactt ggtatttctt acgacgattt ggagttgtac ggaaagtaca
    66301 aggctaaact cagctttgat aaaattcgtg cagttgagag caatccagtt ggtaaattga
    66361 tcttggttac tgccatcaac ccaacacctg caggtgaagg aaagtcgacg cttaccattg
    66421 gtcttgcgga tgccttgaac aagattggca agaaaaccat gattgctatc cgcgaaccgt
    66481 ctcttggtcc agtcatgggg atcaagggtg gtgctgctgg tggtgggtat gcacaagttc
    66541 tgccaatgga agacatcaac ctccacttta ctggagatat gcatgctatt acaactgcca
    66601 acaatgccct ttctgccttg attgacaacc acttgcacca agggaatgag ctgggaattg
    66661 atcaacgtcg tatcctctgg aaacgcgttg tggacttgaa cgaccgtgcg ctccgccatg
    66721 tgactgttgg tcttggtggt cctctaaacg gtattccacg tgaggatggt tttgatatta
    66781 cagttgcttc agaaatcatg gcaattcttt gcttggcaac ggacatcgag gacttgaaac
    66841 gtcgtttggc gaatatcgtt attggttatc gctatgaccg tacgcctgtt tctgtaggtg
    66901 atttgcaggt tgagggtgcc ttggctttga ttttgaagga tgctattaag ccaaacttgg
    66961 ttcagacaat ttacggtaca cctgcctttg tacacggtgg tccatttgcc aatatcgctc
    67021 atggctgtaa ctctgttttg gcgacgacaa cagcccttca cttggctgat tacactgtta
    67081 ctgaagctgg ttttggtgcg gaccttggtg ctgagaaatt ccttgatatc aagacaccaa
    67141 acttgccaac atctccagat gcagttgtta ttgtcgcaac ccttcgtgcc cttaagatga
    67201 atggtggtgt ggctaaagac gctctgactg aagaaaatgt agatgcagtt cgtgcaggtt
    67261 ttgctaactt gaaacgccac gttgaaaata tccgtaagtt cggtattcca gcagttgtag
    67321 ctattaacga atttgtatct gatacagaag ctgaaattgc agccttaaaa gaactctgtg
    67381 cctcaatcga tgtaccagtt gaattggcta gtgtctgggc tgatggagca gaaggtggag
    67441 tagcacttgc cgaaacagtt gttaagacaa ttgctgaaaa tccagctaac tataaacgtt
    67501 tgtacgataa tgacctttct gtccaagaaa agattgaaaa aattgtcact gaaatctatc
    67561 gtggtagcaa agtgaacttt gagaagaaag ctcaaacaca aattgctcaa atcgttcaaa
    67621 atggttgggg caaattgcca atttgtatgg ctaaaactca atacagtttc tcagacaatc
    67681 caaatgcgct tggagcacca gaaaactttg aaattaccat tcgtgaattg gtaccaaaat
    67741 taggtgcagg cttcatcgtt gccttaactg gtgatgttat gaccatgcca ggtcttccaa
    67801 aacgtccagc agctctcaac atggatgttg aaagcgatgg aactgtacta ggcttgttct
    67861 agtggattga aattgacttt aagtgagttt ggaaataata tccaagctca ttttttctca
    67921 gactgcaagc agttcatcaa taagatttat actcttcgaa aatctcttca aactacgtca
    67981 gcttcacctt gccgtaggta tatgatactg actctgtcag ttctatccac aacctcaaaa
    68041 cagtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    68101 aagtagatag aaaagttata tactatctat tgaaaatatt ataaatcaga tacaaagaca
    68161 ggtgaaacct tctgtgttat actagagata tgttagattt gaaagaatac ggtatcgtca
    68221 tgtggccgga ggagaaagtc atttctttcc gtgagaaact tctcgcttgg tatgatgaaa
    68281 acaaaagaga tttgccttgg aggagaagta aaaatcctta tcacatctgg gtatctgaaa
    68341 tcatgcttca gcagaccagg gtggatacag ttatccctta ctacgaaaga ttcctggact
    68401 ggtttccaac tgtcgaaagt ctggcaactg cgtctgagga gagtttactg aaagcttggg
    68461 agggcttggg ctattattct cgagttcgca atatgcaggc tgcagcccag cagattatga
    68521 ctgactttgg tggccaattt ccaaatacct atgaaggaat ttccagcttg aaagggattg
    68581 gaccttacac agcaggagcc atttccagta ttgcttttaa cttgcctgag ccagctgtag
    68641 atggtaatgt catgcgggtc ttggcgcgtc tgtttgaagt caaccacgat attgggattc
    68701 caagtaatcg aaaaattttt caggcaatga tggagatctt gattaatccg gatcgtccgg
    68761 gtgattttaa tcaagccttg atggacttag ggtctgatat tgagtctcct gtaaatccca
    68821 gacctgaaga aagcccagtc aaggacttta gtgcggcata tcagaatgga accatggacc
    68881 gttatccaat taaatctcct aagaaaaagc cggttcctat ttatcttaaa gccttagttg
    68941 tgaaaaattc tcaaggacaa tttttacttg aaaaaaatga aagcgaaaag ctcttggcag
    69001 gtttttggca tttccccttt atagaagttg ataacttttc gcaagaagag cagtttgacc
    69061 tctttcatca ggttgcggaa gaaagtgtga actttggtcc cagtccagaa gagagtttcc
    69121 agcaggacta tgacctagat gttgattggc ttgatgtttg ttttgataca gtccagcatg
    69181 tttttagtca tcgaaaatgg catgtacaga ttgtagcagg tcaggtgagt gacttccatg
    69241 atttttcaga tagggaagtt cgttggcttt caccagaaga attcaagaat tacccacttg
    69301 ccaaacccca acaaaaaatc tggcaggctt atgtacaagc caacttagac agtagccaag
    69361 actagctatt gtgtcttctg actatttttt ggtataatag tagagaaaaa ggtgaacata
    69421 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
    69481 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg ctagagcaat
    69541 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
    69601 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
    69661 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
    69721 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
    69781 ctatgccagt agatgggcag gaagcagata gtaaacctca acctatccaa attggggatt
    69841 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
    69901 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
    69961 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
    70021 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
    70081 tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
    70141 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
    70201 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
    70261 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
    70321 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
    70381 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
    70441 gcgactaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
    70501 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
    70561 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatatc
    70621 aatggcgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
    70681 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
    70741 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
    70801 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
    70861 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
    70921 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
    70981 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
    71041 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
    71101 acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga tgggggtaaa
    71161 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
    71221 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
    71281 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatt
    71341 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
    71401 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
    71461 gactagaatg agtgaaatag gctttaaata cagtatttta gcatcgggtt ccagtggaaa
    71521 ttctttttat ctggaaacct caaaaaagaa gcttttagtg gatgcaggct tgtctggcaa
    71581 gaaaattacc agtctgctag ctgaaattaa ccgcaagcca gaagacctgg atgccatctt
    71641 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
    71701 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
    71761 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cctttggaga
    71821 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
    71881 tatgaaggat gataagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
    71941 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
    72001 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttgggca
    72061 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gtactaagaa
    72121 gatttacctt gggcatttat ctaaggaaaa taatatcaag gaactggctc atatgaccat
    72181 ggtcaatcag ctggctcaag ctgacctggg agtcggagta gactttaagg tttatgatac
    72241 ctcaccagat accgcaacac cattgacaga gatatagaaa gaacgctgag aaggtgttct
    72301 ttttatattg actgaacacc caaaaagtaa tacaatggtg ttaccattaa aaaagggagc
    72361 acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat ttatgaaagc
    72421 aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa ctcttgaagc
    72481 cctagaagat gaatacgatg ctcgtgtggc agatttagcc tatcaagagt atttagaaga
    72541 tttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt taggcttgaa
    72601 ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa ttgaaaaaat
    72661 tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt ttggaagacc
    72721 caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc tatagaattg
    72781 gtaattatcg agttatcgta caaattgtag atgatgaatt agttgttgct actctagaag
    72841 ttggtcatcg gagagatatt tattgaattc aagctgagta ataacagtat agaaagtaag
    72901 gagagtaaaa atttgaagaa aaaatggtta tttgcagatt attatgatac aacaattatt
    72961 ttacttgctt taatctctgt tattctggta ttacttggat ttgctgagat gactgatttg
    73021 gacaatccgc cttatagtat tattgattta gtaatctggg gtgtttttgt aattgattat
    73081 agttggcgct tttttattac taaaagaaaa tggcgcttta ttcttgaaaa tatttttgat
    73141 ttattggcta ttctcccttt aaatgctatt tttacagtgt ttcgattagg acgtatcttc
    73201 cgcttagcaa ggctgacaaa attactgaaa ctgactcgtt tacttagaat aattggactt
    73261 acaggtaaac tagaaagaaa aattagtaga ttcttacgaa caaatggatt gatttatatc
    73321 ttgtatgtta atatctttat tgttctagta gggagttcga ttttatccgt tgttgaagaa
    73381 aaatccttct cagatagtct ttggtgggct cttgtgacag taactactgt tggttatggt
    73441 gatattgttc cagtttccct ttttgggaaa tggctagcag ttttactgat gcttgtcggt
    73501 attggaacaa tagggatgct aacaagtgct ttgacgaact tttttgtaaa ggataatcca
    73561 gatgaacaga taaaacttga taaactcaaa gacgaattat cgagctaaag aatattaata
    73621 gagaaacaat ctgaaaaaat cgaagaacta cattgaatga taaaagactt agttgaaaaa
    73681 atatcataaa ggtgagacaa aataaaactt gattcgagga gaaatcaagt aagttttgac
    73741 acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta tagagaaata
    73801 aaataaaatc tgaacaaatc aattaggaat ttaaagcatt ttataacaac gttttagagt
    73861 aatggggtgc tatttcaact tcaacctact ataatacaga aaaaaataca gaaaaaaaca
    73921 actccctgat gattcaagga gttgtctata gttaaattag tttttagaag cttcttggaa
    73981 ttctggattt ttccatgctt cgtcaataat agcttgcaat tctttagcag atgcttgcat
    74041 tttttgagtt tctgcatcgt tcaatggaat gtttactgga cgaacgatac catgtgcacc
    74101 aacaacagct ggttgaccga taaagacatt ctcaactccg tattgacctt cttggaatac
    74161 tgaaagtgga agtactgcgt tttcatcgtc aaggattgct ttagtgatac gagcaagggc
    74221 tactgcgata ccgtagtatg ttgcaccttt tttgttgatg attgtgtagg ctgcatcacg
    74281 aacaccttcg aacaattcaa tcaattcagc ttcttgaaca ttttgagtgt ctttaaggaa
    74341 ttcttcaagg tttacaccag cgatgttagc gtgtgaccaa acagcgaact cagagtcacc
    74401 gtgttcaccc atgatgtagg cgtgcactga acgagcatcc acatccaatt tttcagcaag
    74461 tgcttgacgg aaacgagctg agtcaagtga agtacctgaa ccgataacgc gttctttagg
    74521 gaaaccagag aatttccaag ttgagtaagt caaaacgtca actgggttag cagcaacaag
    74581 gaagatacct ttgaaaccag attcaacaac ttgagttacg attgatttgt tgatagcaag
    74641 gtttttacct acaaggtcaa gacgagtttc acctggtttt tgaggtgcac ctgcagtgat
    74701 tacaacaagg tcagcgtctg cacagtcaga gtattgagct gcatagattt ttttaggtga
    74761 agtgaaggca agggcgtgac taaggtcaag cgcatcacca acagcttttt catgcaattg
    74821 tggaatttcg ataattccaa gctcttgtgc aattccttgg ttaacaagtg caaaagcgta
    74881 agatgaacct acagcaccat caccgacaag gataactttt ttgtgttgtt tagttgaagt
    74941 cattgtttta aacatctcct taattttatt aggggatttt ccctagacaa cttcattcta
    75001 tcacttttaa aaaactttgt cacgaatatg ccttatagtt ctcgatgtaa acgttttagt
    75061 ggtttagagg ctgaaataga tgggaattta tggtataatg ttgttactta ctaattgtga
    75121 aatgaggcat ttattaatgc aggataaaaa tttagtgaat gtcaatctga caaaggagat
    75181 gaaggcaagt tttatcgact acgccatgag tgttatcgta gcgcgagctc ttcctgatgt
    75241 tcgagatggc ttaaaacctg ttcaccgtcg cattctctac ggaatgaatg aattgggtgt
    75301 gaccccagac aaaccccata aaaaatctgc tcgtattaca ggggatgtca tgggtaaata
    75361 tcacccacac ggggattcct ctatttatga agccatggtc cgtatggctc aatggtggag
    75421 ctaccgttac atgcttgtag atggtcatgg gaattttggt tccatggatg gagatagtgc
    75481 tgccgctcaa cgttataccg aggcacgtat gagcaagatt gctctggaaa tgcttcgtga
    75541 tatcaacaaa aatacagttg atttcgttga taactatgat gccaatgaac gggaaccctt
    75601 ggtcttgcca gcgcgttttc caaacctttt ggttaatgga gcaactggta tcgcggttgg
    75661 gatggcaacc aatattccac ctcataatct gggtgaaacc attgatgcag tgaagttggt
    75721 catggataat cctgaagtga ctaccaagga cttgatggaa gtcttgcctg gaccagattt
    75781 tccaactggt gctcttgtca tggggaaatc aggtatccat aaggcttatg aaacaggtaa
    75841 aggttcgatt gtcctacgtt ctcgtacaga gattgaaacg actaagactg gtcgtgagcg
    75901 tatcgttgta acagaatttc cttacatggt caataaaacc aaggtgcatg agcatattgt
    75961 tcgcttggtt caggaaaaac gcattgaggg tatcacagca gtacgtgatg agtcaaaccg
    76021 tgaaggtgtt cgatttgtta ttgaagtcaa gcgcgacgcc tcagccaatg ttattctcaa
    76081 taacctcttc aaaatgaccc aaatgcaaac caattttggt ttcaatatgc tcgctatcca
    76141 aaatggtata ccgaaaattt tgtctcttcg tcagattttg gatgcttata tcgagcacca
    76201 aaaagaagtg gttgttcgtc gtacacgttt tgataaggaa aaagcggaag cgcgcgctca
    76261 tatcttagaa ggtctcttga ttgcgctaga ccatatcgac gaagtgattc gtatcatccg
    76321 tgctagtgaa acggatgcgg aagctcaagc tgagttgatg agcaagttta agctttctga
    76381 acgtcaaagt caagctatcc ttgatatgcg tcttcgtcgt ttgacaggtt tggaacgcga
    76441 taagattcaa tctgagtatg atgacctctt ggctctgatt gcggatttag cagatattct
    76501 tgctaagcct gaacgtgttt ctcaaattat caaagacgaa ttggatgaag ttaaacgtaa
    76561 attttctgat aaacgccgta cagagttgat ggttggacag atcttgagtc tcgaggatga
    76621 ggacttgatt gaagaatcgg atgtcttgat taccctttct aacagaggct acattaagcg
    76681 tttggatcag gacgagttca ctgctcaaaa acgtgggggt cgtggtgtcc aaggaacggg
    76741 agtgaaagat gatgactttg ttcgtgagtt agtgtcaact agcacccatg atcatctgct
    76801 cttcttcaca aacaagggac gtgtctatcg tcttaaaggt tatgaaattc ctgagtatgg
    76861 tcggactgcc aaagggctac cagtagtcaa tctcttgaaa ttggatgaag atgaaagtat
    76921 tcagacggtt atcaatgttg agtctgatcg cagtgatgat gcttatctct tctttacaac
    76981 ccgtcacggt attgtgaaga gaactagtgt taaggagttt gccaatattc gtcaaaatgg
    77041 tctcaaagcg ctgaatttaa aggatgaaga tgagttaatc aatgtcttgt tgacagaagg
    77101 agatatggat attatcattg gtaccaagtt tggttatgca gttcgcttta atcaatcagc
    77161 cgttcgtggt atgagccgta tcgccactgg tgtgaaaggt gttaaccttc gtgaaggaga
    77221 cacagttgtt ggtgccagct tgattactga tcaagatgag gttcttatta tcacagaaaa
    77281 aggatatggt aagcgtacag tcgctactga atacccaaca aaaggtcgtg gtggtaaggg
    77341 aatgcagaca gctaaaatta ccgaaaaaaa tggcttgctg gccggtctta tgactgttca
    77401 aggtgatgag gatttgatga ttatcactga tacaggtgtc atgattcgaa ccaatcttgc
    77461 caatatttca caaacaggac gtgcaactat gggagttaaa gtaatgcgcc tggatcaaga
    77521 tgctcagata gtgactttca caacggttgc ggtggcagaa aaagaagaag ttgggacaga
    77581 aaacgaaaca gaaggtgaag cataatgtct caaaaaaata ataaaaagaa aaacaagcga
    77641 aaaaatctgc tgacaaatat cctagcagga tttctgatat tactgtcact ggctttgatt
    77701 tttaatactc aaattcgaaa tattttcata gtctggaata ccaataagta tcaagttagc
    77761 caggtatcaa aagaaaaatt agaagaaaat caggatacag aaggcaattt tgactttgat
    77821 tctgtcaaag ctatctcttc ggaagctgtt ctaacttctc aatgggatgc tcaaaaatta
    77881 ccagttattg ggggaattgc aattcctgaa ttggaaatga atttgccgat ttttaaagga
    77941 cttgataatg ttaatctctt ctacggagct ggtacaatga aacgcgagca agtaatggga
    78001 gaaggaaatt atagtctagc tagtcaccat atctttggtg ttgataatgc taataaaatg
    78061 ttattttctc ctttagataa tgctaaaaat ggcatgaaga tttatctaac cgataaaaat
    78121 aaagtttata cttatgaaat acgtgaagtc aaacgtgtga caccggatcg tgttgatgaa
    78181 gttgatgata gagatggggt caatgaaatc acattagtaa cctgtgaaga ccttgctgct
    78241 acagaacgta ttattgtcaa aggtgatttg aaagaaacaa aagattattc acaaacatct
    78301 gatgaaatcc taacagcttt caatcaacca tataaacaat tttattaata caaatcagtg
    78361 aaatcatgga tttcactgat tttttaagat tttcataaaa atatagtaaa atgaaataag
    78421 aataggacga atcg
//
LOCUS       DBERCA010000004        73500 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.004, whole genome shotgun sequence.
ACCESSION   DBERCA010000004 DBERCA010000000
VERSION     DBERCA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 73500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.004"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(48..1937)
                     /locus_tag="ACL719_000280"
     CDS             complement(48..1937)
                     /locus_tag="ACL719_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGS0698315.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2075..3640
                     /locus_tag="ACL719_000281"
     CDS             2075..3640
                     /locus_tag="ACL719_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS0698316.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKKPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3640..4404
                     /locus_tag="ACL719_000282"
     CDS             3640..4404
                     /locus_tag="ACL719_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0698317.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4603..4818)
                     /locus_tag="ACL719_000283"
     CDS             complement(4603..4818)
                     /locus_tag="ACL719_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698318.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="ACL719_000284"
     CDS             complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="ACL719_000284"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGS0698319.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6989..7345)
                     /locus_tag="ACL719_000285"
     CDS             complement(6989..7345)
                     /locus_tag="ACL719_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS0698320.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7583..7819)
                     /locus_tag="ACL719_000286"
     CDS             complement(7583..7819)
                     /locus_tag="ACL719_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698321.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="ACL719_000287"
     CDS             complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="ACL719_000287"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS0698322.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="ACL719_000288"
     CDS             complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="ACL719_000288"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGS0698323.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9483..11183)
                     /locus_tag="ACL719_000289"
     CDS             complement(9483..11183)
                     /locus_tag="ACL719_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGS0698324.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11384..13051)
                     /locus_tag="ACL719_000290"
     CDS             complement(11384..13051)
                     /locus_tag="ACL719_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGS0698325.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13054..13419)
                     /locus_tag="ACL719_000291"
     CDS             complement(13054..13419)
                     /locus_tag="ACL719_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0698326.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13576..13764)
                     /gene="rpmB"
                     /locus_tag="ACL719_000292"
     CDS             complement(13576..13764)
                     /gene="rpmB"
                     /locus_tag="ACL719_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGS0698327.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13880..14056)
                     /locus_tag="ACL719_000293"
     CDS             complement(13880..14056)
                     /locus_tag="ACL719_000293"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0698328.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14167..14364)
                     /locus_tag="ACL719_000294"
     CDS             complement(14167..14364)
                     /locus_tag="ACL719_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698329.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(14792..16336)
                     /locus_tag="ACL719_000295"
     CDS             complement(14792..16336)
                     /locus_tag="ACL719_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGS0698330.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLELLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16549..16851
                     /gene="gatC"
                     /locus_tag="ACL719_000296"
     CDS             16549..16851
                     /gene="gatC"
                     /locus_tag="ACL719_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGS0698331.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            16851..18317
                     /gene="gatA"
                     /locus_tag="ACL719_000297"
     CDS             16851..18317
                     /gene="gatA"
                     /locus_tag="ACL719_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGS0698332.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAHVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TIQSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18317..19759
                     /gene="gatB"
                     /locus_tag="ACL719_000298"
     CDS             18317..19759
                     /gene="gatB"
                     /locus_tag="ACL719_000298"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGS0698333.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEIQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEIAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19888..20448
                     /gene="efp"
                     /locus_tag="ACL719_000299"
     CDS             19888..20448
                     /gene="efp"
                     /locus_tag="ACL719_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGS0698334.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20470..20859
                     /locus_tag="ACL719_000300"
     CDS             20470..20859
                     /locus_tag="ACL719_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0698335.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20852..21274
                     /gene="nusB"
                     /locus_tag="ACL719_000301"
     CDS             20852..21274
                     /gene="nusB"
                     /locus_tag="ACL719_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGS0698336.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21324..21560)
                     /locus_tag="ACL719_000302"
     CDS             complement(21324..21560)
                     /locus_tag="ACL719_000302"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS0698337.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21573..22047)
                     /locus_tag="ACL719_000303"
                     /pseudo
     CDS             complement(21573..22047)
                     /locus_tag="ACL719_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22065..22250)
                     /gene="briC"
                     /locus_tag="ACL719_000304"
     CDS             complement(22065..22250)
                     /gene="briC"
                     /locus_tag="ACL719_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGS0698338.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(22409..23176)
                     /locus_tag="ACL719_000305"
     CDS             complement(22409..23176)
                     /locus_tag="ACL719_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGS0698339.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(23173..24039)
                     /gene="accD"
                     /locus_tag="ACL719_000306"
     CDS             complement(23173..24039)
                     /gene="accD"
                     /locus_tag="ACL719_000306"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGS0698340.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(24076..25443)
                     /gene="accC"
                     /locus_tag="ACL719_000307"
     CDS             complement(24076..25443)
                     /gene="accC"
                     /locus_tag="ACL719_000307"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGS0698341.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25455..25877)
                     /gene="fabZ"
                     /locus_tag="ACL719_000308"
     CDS             complement(25455..25877)
                     /gene="fabZ"
                     /locus_tag="ACL719_000308"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGS0698342.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(25874..26359)
                     /gene="accB"
                     /locus_tag="ACL719_000309"
     CDS             complement(25874..26359)
                     /gene="accB"
                     /locus_tag="ACL719_000309"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGS0698343.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26362..27597)
                     /gene="fabF"
                     /locus_tag="ACL719_000310"
     CDS             complement(26362..27597)
                     /gene="fabF"
                     /locus_tag="ACL719_000310"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGS0698344.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27619..28350)
                     /gene="fabG"
                     /locus_tag="ACL719_000311"
     CDS             complement(27619..28350)
                     /gene="fabG"
                     /locus_tag="ACL719_000311"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGS0698345.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(28384..29304)
                     /gene="fabD"
                     /locus_tag="ACL719_000312"
     CDS             complement(28384..29304)
                     /gene="fabD"
                     /locus_tag="ACL719_000312"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGS0698346.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(29297..30271)
                     /gene="fabK"
                     /locus_tag="ACL719_000313"
     CDS             complement(29297..30271)
                     /gene="fabK"
                     /locus_tag="ACL719_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGS0698347.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(30390..30614)
                     /locus_tag="ACL719_000314"
     CDS             complement(30390..30614)
                     /locus_tag="ACL719_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS0698348.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30674..31648)
                     /locus_tag="ACL719_000315"
     CDS             complement(30674..31648)
                     /locus_tag="ACL719_000315"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGS0698349.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31648..32082)
                     /locus_tag="ACL719_000316"
     CDS             complement(31648..32082)
                     /locus_tag="ACL719_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS0698350.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(32176..32961)
                     /locus_tag="ACL719_000317"
     CDS             complement(32176..32961)
                     /locus_tag="ACL719_000317"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGS0698351.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33286..34650
                     /locus_tag="ACL719_000318"
     CDS             33286..34650
                     /locus_tag="ACL719_000318"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGS0698352.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34653..35024
                     /locus_tag="ACL719_000319"
     CDS             34653..35024
                     /locus_tag="ACL719_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGS0698353.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            35238..36512
                     /gene="serS"
                     /locus_tag="ACL719_000320"
     CDS             35238..36512
                     /gene="serS"
                     /locus_tag="ACL719_000320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGS0698354.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDTKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36537..37436)
                     /locus_tag="ACL719_000321"
     CDS             complement(36537..37436)
                     /locus_tag="ACL719_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGS0698355.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37546..38094)
                     /locus_tag="ACL719_000322"
     CDS             complement(37546..38094)
                     /locus_tag="ACL719_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGS0698356.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38312..39634)
                     /locus_tag="ACL719_000323"
     CDS             complement(38312..39634)
                     /locus_tag="ACL719_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGS0698357.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39725..39813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39977..42313)
                     /locus_tag="ACL719_000324"
     CDS             complement(39977..42313)
                     /locus_tag="ACL719_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGS0698358.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEKLARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42417..42965)
                     /locus_tag="ACL719_000325"
     CDS             complement(42417..42965)
                     /locus_tag="ACL719_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGS0698359.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42962..43264)
                     /gene="zapA"
                     /locus_tag="ACL719_000326"
     CDS             complement(42962..43264)
                     /gene="zapA"
                     /locus_tag="ACL719_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGS0698360.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43351..44232
                     /gene="rnhC"
                     /locus_tag="ACL719_000327"
     CDS             43351..44232
                     /gene="rnhC"
                     /locus_tag="ACL719_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGS0698361.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEKKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            44236..44850
                     /gene="lepB"
                     /locus_tag="ACL719_000328"
     CDS             44236..44850
                     /gene="lepB"
                     /locus_tag="ACL719_000328"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGS0698362.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            45129..47495
                     /locus_tag="ACL719_000329"
     CDS             45129..47495
                     /locus_tag="ACL719_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGS0698363.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47543..48826)
                     /gene="tig"
                     /locus_tag="ACL719_000330"
     CDS             complement(47543..48826)
                     /gene="tig"
                     /locus_tag="ACL719_000330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGS0698364.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(49020..49379)
                     /locus_tag="ACL719_000331"
     CDS             complement(49020..49379)
                     /locus_tag="ACL719_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0698365.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(49681..50271)
                     /gene="cbpG"
                     /locus_tag="ACL719_000332"
                     /pseudo
     CDS             complement(49681..50271)
                     /gene="cbpG"
                     /locus_tag="ACL719_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
     gene            complement(50352..50591)
                     /locus_tag="ACL719_000333"
     CDS             complement(50352..50591)
                     /locus_tag="ACL719_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698366.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(50741..51283)
                     /locus_tag="ACL719_000334"
                     /pseudo
     CDS             complement(50741..51283)
                     /locus_tag="ACL719_000334"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(51265..51507)
                     /locus_tag="ACL719_000335"
     CDS             complement(51265..51507)
                     /locus_tag="ACL719_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HGS0698367.1"
                     /translation="MSLADLLEELEAAKDSKKARPMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(51508..52140)
                     /locus_tag="ACL719_000336"
     CDS             complement(51508..52140)
                     /locus_tag="ACL719_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS0698368.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(52154..53149)
                     /locus_tag="ACL719_000337"
     CDS             complement(52154..53149)
                     /locus_tag="ACL719_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0698369.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLDQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(53146..53844)
                     /gene="liaF"
                     /locus_tag="ACL719_000338"
     CDS             complement(53146..53844)
                     /gene="liaF"
                     /locus_tag="ACL719_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGS0698370.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKNYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(53922..54932)
                     /gene="fni"
                     /locus_tag="ACL719_000339"
     CDS             complement(53922..54932)
                     /gene="fni"
                     /locus_tag="ACL719_000339"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGS0698371.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLHTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVLGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(54916..55923)
                     /locus_tag="ACL719_000340"
     CDS             complement(54916..55923)
                     /locus_tag="ACL719_000340"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGS0698372.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(55910..56863)
                     /gene="mvaD"
                     /locus_tag="ACL719_000341"
     CDS             complement(55910..56863)
                     /gene="mvaD"
                     /locus_tag="ACL719_000341"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGS0698373.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(56845..57723)
                     /gene="mvk"
                     /locus_tag="ACL719_000342"
     CDS             complement(56845..57723)
                     /gene="mvk"
                     /locus_tag="ACL719_000342"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGS0698374.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(57845..58861)
                     /gene="cbpC"
                     /locus_tag="ACL719_000343"
     CDS             complement(57845..58861)
                     /gene="cbpC"
                     /locus_tag="ACL719_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGS0698375.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGLTWYYLNAGNGDMKTGWFQVNGKWYYAYSSGALAVNTTVDGYSVNYNG
                     EWVQ"
     gene            complement(58959..59648)
                     /locus_tag="ACL719_000344"
     CDS             complement(58959..59648)
                     /locus_tag="ACL719_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0698376.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(59660..61084)
                     /gene="gndA"
                     /locus_tag="ACL719_000345"
     CDS             complement(59660..61084)
                     /gene="gndA"
                     /locus_tag="ACL719_000345"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGS0698377.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(61160..62554)
                     /gene="mapZ"
                     /locus_tag="ACL719_000346"
     CDS             complement(61160..62554)
                     /gene="mapZ"
                     /locus_tag="ACL719_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGS0698378.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(62566..63723)
                     /locus_tag="ACL719_000347"
     CDS             complement(62566..63723)
                     /locus_tag="ACL719_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGS0698379.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(63808..64188)
                     /gene="rnpB"
                     /locus_tag="ACL719_000348"
     ncRNA           complement(63808..64188)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL719_000348"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(64209..64538)
                     /gene="gpsB"
                     /locus_tag="ACL719_000349"
     CDS             complement(64209..64538)
                     /gene="gpsB"
                     /locus_tag="ACL719_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGS0698380.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(64608..65135)
                     /locus_tag="ACL719_000350"
     CDS             complement(64608..65135)
                     /locus_tag="ACL719_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGS0698381.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            65201..65797
                     /gene="recU"
                     /locus_tag="ACL719_000351"
     CDS             65201..65797
                     /gene="recU"
                     /locus_tag="ACL719_000351"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGS0698382.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            65794..67953
                     /gene="pbp1a"
                     /locus_tag="ACL719_000352"
     CDS             65794..67953
                     /gene="pbp1a"
                     /locus_tag="ACL719_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGS0698383.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAA
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(68120..73423)
                     /locus_tag="ACL719_000353"
     CDS             complement(68120..73423)
                     /locus_tag="ACL719_000353"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGS0698384.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTASTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSIKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HSRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKLVVTRKSNDVNSPLYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYSFVVGKGEFQSGRRGTQASNLEIHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FESLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
BASE COUNT        21373 a        16891 c        13653 g        21583 t
ORIGIN      
        1 ttctatcttc cattatatca tactttttca gtctatggta gctctcttta aaaggcaccg
       61 atactgccgc cacctccgcc tccagagaag ccaccaccag aacttccact tccagaagat
      121 acagagtagg tgcttgctgt atttgcgaca ctagcataat ggctcatttg cgcgcttgaa
      181 tgataaaaca gactgtgcca gccataagct acatagagat tgatatctgg attttccact
      241 tgaatctgat gaaccttcat caaatgacta accttgtccg catagccaaa taaggtcgca
      301 tagaccaaga ggcgattcca gaccacaata ctttccagtt cagcctgatc caatcgtgca
      361 atctcacgca acatattttc aaaactggtc cagagatagt agacctcagc tcccgcttca
      421 tttagaacac catcacgatt atctagtcga agcttccaat aatagaaaac agacaaaact
      481 aaccctagaa aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg
      541 tacaagaaca aaccaaatcc gataaatagg ggcaggatag tcaaggcacc catacccact
      601 tgcaaggcct tttccccacc agttaaagga cgataataat ctgggagccc ccagaaggaa
      661 actcgttttc tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagt
      721 tgaagccctc ttgcttgaat ccgtttttca tcagaaactt tggctctacg ataaagacta
      781 tcagatacct tgtaatccgc aaacaaattg gaaagagttt cttctttttt acctgaaaaa
      841 gctagattta ggcagtcttt ctcaaagctt gacaaaccat cttcttttac tagcctcaaa
      901 ccaactgcat ctccttctga aatgatagag acattcccac ggtctatcac atctagcaag
      961 gtagcttgaa taagttgatc aaaggtgaat tttccagctc ccttgaccaa gggactcact
     1021 tcctccaagg aggtcgagta gactgcttct gataaaacca taggctctaa ttccattggt
     1081 ggttcataga gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa
     1141 ataaaataga agcagacact caataacaag gagatagaaa ggatcgaagg gaggacccaa
     1201 gtaacaagtt gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac
     1261 tcttctaaac gattcccttt caatccctga tccctagcgc tagcaaaatc ggtccgaggc
     1321 caataggcat gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa
     1381 tcaaggttac tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt
     1441 ttttcagctc ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta
     1501 tctgtcagag gttgccaatt taattcagcg atatcatcat aaaggaaaag taaatttttt
     1561 aagttccaga cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc
     1621 acagtataac catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg
     1681 tttttcgcgg cctgaacctt tggatgaggg tcaatgtcaa acccgctagg catcttacca
     1741 gcacgtccaa gtcccacgat ttgtccctta aagtcctcct caaactggta aactatcttc
     1801 tgtctaaact ctgccgtatt atctgcatga atatacaaat ccccttgata agagtttatc
     1861 ttgaaatcaa tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag
     1921 aaaaaagttt ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt
     1981 ttcgtaagta aaggcttact tatgttgaaa aattacgcat ttttcgataa aataggagac
     2041 agttattttt taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt
     2101 cacaagtctc ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg
     2161 catcgggatg gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg
     2221 agctgtcaaa atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa
     2281 gaaaatcgct aaggacttag gtaaaaaacc tttggttgtt aaaaccaagt gggaaggtct
     2341 agtccctgcc cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc
     2401 agaacgcaaa caagaaattg ccttttcgag cagttactac actagcgaac cagttttgct
     2461 tgtcaaaaaa gattctgcct acgcaagtgc taaatctttg gatgacttta acggtgcaaa
     2521 aatcacttct caacaagggg tctacctcta cgacttgatt gcacaaatcc caggtgctaa
     2581 aaaagaaaca gccatgggag acttcgctca aatgcgacaa gctcttgagg ctggtgtcat
     2641 tgatgcttat gtttctgaac gtccagaagc actgactgct gaagctgcga actctaagtt
     2701 caagatgatt caagtagaac ctggtttcaa aactggggaa gaagatacag ctatcgctat
     2761 cgggcttcgt aaaaatgaca atcgtattag ccaaatcaat gccagcattg aaaccatttc
     2821 aaaagatgac caagttgcct tgatggatcg tatgatcaag gaacaacctg ccgaagctac
     2881 aacaactgaa gagactagca gtagtttctt tagccaagtc gctaaaattc tttctgaaaa
     2941 ctggcaacaa ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat
     3001 cataggtctc attattggac ttgccattgg tgtcttccgt actgctcctc tctctgaaaa
     3061 caaagccatt tatggccttc aaaaactagt cggctgggtt ctcaatgtct acattgaaat
     3121 tttccgtggt acgccaatga ttgttcaatc gatggttatc tactatggaa ctgcccaagc
     3181 tttcggtatc aaccttgacc gtacactagc tgctatcttc atcgtttcaa tcaataccgg
     3241 tgcctacatg actgaaattg tccgtggtgg tatcctggca gttgacaagg gacaatttga
     3301 agctgcgact gctcttggta tgactcataa ccaaaccatg tgtaagattg tcctacctca
     3361 ggttgtccgc aatatcctac cagcaactgg taatgaattt gtcatcaata tcaaagatac
     3421 atctgtattg aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac
     3481 acaaacctat caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac
     3541 cttcaccgta acacgtatcc tacgctttat cgagcgcaga atggacatgg atacctacac
     3601 tacaggtgct aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa
     3661 attaaacacc tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttctctcact
     3721 gtccacaagg gagaagttat ttctatcatc ggaagctctg gaagtgggaa atcaaccttc
     3781 ctacgctcca ttaacctact tgaaacgcca actgatggac aaatccttta tcatggacaa
     3841 aacgtcctcg aaaaaggcta tgacctcacg caataccgtg aaaagttagg gatggttttc
     3901 caatccttta acctctttga aaatctcaac gttcttgaaa acacaatcgt cgctcagaca
     3961 actgtcctta aacgcgaacg cacagaagct gaagaaattg ccaaagaaaa cctggaaaag
     4021 gtcggcatgg gagaacgcta ctggcaagcg agacccaaac aactctcagg tggtcaaaaa
     4081 caacgtgtgg ccatcgctcg tgccctttca atgaatcctg acgccattct ctttgatgaa
     4141 ccaacatcag ctctcgatcc agaaatggtt ggagaggtcc tcaaaatcat gcaagatctg
     4201 gctcaggaag gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc
     4261 tctcaccgtg ttatctttat ggataagggt gtgattgctg aagaaggtaa accagaagac
     4321 ctcttcacta atcctaaaga agaccgtaca aaagaatttc ttcaacgtta tctcatactc
     4381 aatgaaaatc aaagagcaaa ctaggcagct agccgcaggt tgctcaaaac actgttttga
     4441 ggttgcagat agagctgacg tggtttgaag agattttcga agagtatcaa ataaaaagaa
     4501 aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc agattctcta
     4561 tctctactac ttgaaggtca agaatccacc gttatccaaa gattaatctt caaatttttc
     4621 atgatttttt tcatagaaat caattaagcc tagggctgtt tcaaatgaaa tattttttac
     4681 ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg tttctttaga
     4741 gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt ctactttttc
     4801 aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt aattgtatac
     4861 gtttttacta cttttgtcaa aaaaaaaaac atatattcaa cgaaaataat tagttcagaa
     4921 ttttcttact tcctttttaa atccgataat atataggttt tgctagcctc tacaccataa
     4981 atatttgcta ataaaaatac tgtattatta ccctcttaag gtgcaaaatt attcaagttc
     5041 ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa gaactgaaga
     5101 tgatagttct aacctttaga aatccaagcc ttctctagct caaaaactcc tatctttctt
     5161 cctcaataaa tcatctgaga gataggtatt ccttatatcc ttcaaattca tcactccttt
     5221 ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc cttaatagag
     5281 atgacaagac agataagctc agttaattac ttatatatct ttttaatatg gtatatttgt
     5341 caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc aaactaggaa
     5401 gctagccgca aattgctcaa aacactgttt tgaggtttca gatagagcta acgtggtttg
     5461 aagagatttt cgaagagtat taaagacagt atgactatgc ttgtcaatag ataggaaatc
     5521 aaaatgaagg atggaaatct gagccaaaca tcataagtgc tataatgaac agaagtaaga
     5581 attgcacgat aagttttcta aaaccatagg aatcgtcccc tttcttcatc ttcattatag
     5641 cacatataca tcagttgtac aaataatatg atattttttt attagtcctc agacaagcat
     5701 attataaagc gtttcattac catttaagtt aatataagct ggatcaaaac cttccattct
     5761 acggatcaaa cctgcataat catgcttatc tgccaaagcg atcccaatca atactgggcc
     5821 tgttcccttg ctagctcgtt tgatatactc aaaacgtgtg atatcatcat ttggccccag
     5881 gatatcattt acaaactcac gcaaagctcc tggacgctgt gggaaattga ccacaaagta
     5941 atgtttgata ccatcataaa tcaaggcacg ctcttccatt tctggcatac ggttgatatc
     6001 attatttcct ccagaaatga tacaacaaat ggttttcccc ttaatatatt cagctaaaac
     6061 ctctaaagag gcgatactag ccgctccagc aggttctgcg actatccctt gcttagagta
     6121 aaggtcaatc aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag tttgaatatg
     6181 ttgacgagtt gcttcatagg tcaactgacc taccttttgc acagcaatcc catccgcaaa
     6241 tttatcaatt tccttgagtt tgactggacc tccagcctca aaggcagctt tcatggaacg
     6301 cgctccattc gcctctactc cgataacctc aatctctgga cttgtttcct tgatataggt
     6361 agaaaccccg gcaatgagac cgccaccacc aacaggaacc aagacagcat caaaatcaat
     6421 cgattctttt cgagcttctt ctaaaatctc ataagcaact gttccttgac ctgcttgaac
     6481 atgagcatca tcaaaaggat caataaaggt acgattttca gagactgtaa attcttgagc
     6541 tgctttggct gaggcatcaa aggtatctcc aactagttta atagttacaa aatccccacc
     6601 aaaaaagcga acctgaccaa tcttttgttg tggcgtagta ataggcataa agatagtagc
     6661 aggaattttc atttcattac aagtataggc tactccctgc gcatgatttc ccgcagaagc
     6721 gcagactacc ccacgttcac gttcttcctt gctgagctgg gaaatggcat aataggcacc
     6781 acgaatttta aaggagcgaa cacgctgggc attttctttt ttcaaataaa tcttagcacc
     6841 atacttctcc gataaataat gatcgtaatc cagtggagta ttcacaacca caccgttcaa
     6901 gaccttgtga gccttgatta tatcttttga acttaacata taaaacctct ttttctataa
     6961 aaaacagtca ctaatgactg ttttttagtt attcatctcc gaaaacttca gtaacacttt
     7021 catggtattt atgagccata cgaatcgaaa actctttagg aagttcataa cgatttgtgg
     7081 aaatccaatc atataaccat tctaccaaac tttcttcact atcattacga tggaagataa
     7141 aatagtttaa aatttcttta tattctacat cattcaaatc caacgtgtct acaaattgac
     7201 tacgaggaac aaaattagaa tctaattttc ttagttgatc tgtcaattct cccaattgta
     7261 aatccaactg aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat cctgtcttga
     7321 tagaaaacaa attggtaata ttcatatgat ttcaacccct aaataagtac atctaatatc
     7381 ccacgaatag ctgaagctaa agttttcgca gtccagtcat ttaaacccga ttctttcaat
     7441 ccagatgcaa taatttgctc tatttgatat aaaccgccat ctatcgtctc atttattctg
     7501 cgaagaggac cttcaatata ggataaagct tctccgaatc taacagctga tttaggtcct
     7561 aatgatttac caactatatt tgttaatgtt ttttatttct caaaatccat ttcgctatat
     7621 aacgagcaga tttagtcgca ccagctctct caaacggaga atgaatttca agaccttctt
     7681 ctctagctat ttgtgccaat ttgaagaatt gttcaaaggt catatgcaac ctaccatctt
     7741 caaaattagc atcatcataa atgttaatta cagcattttc atcatacgac caatctgctt
     7801 tattttggtc tgctagcact attaccgacg gtgtaattac attatacaac tagaaccaat
     7861 aataacgatg caatttttct cataatcttt ttcataaaat catctccagc gctatatatt
     7921 tctaattagt tatagatttt gaatgcatca tcgtcgtttt taccaacaaa tggcattgct
     7981 ttacgcaatt ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc acggtaagca
     8041 gtcaattttg gacgtccagc tttatagtca tttacaaagt catttgcaaa tttaccattt
     8101 tggatgtctg ccaagacagc cttcatattt tctttaactt gttcagtgat tacacgtgga
     8161 cctgatacat agtcaccgta ttcagcagtg tttgaaatag attgacgcat tttcttgaat
     8221 ccaccttcgt agatcaagtc aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc
     8281 aattctggag cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc
     8341 aaaccaccac aaagtacagc ttgttcacca aacaaatctt cttcagtttc ttctttgtaa
     8401 gttgtttcaa gaagacctac acgagccgct ccaacacctt tacaccagtc catagcaatg
     8461 tttttagcat ttcctgttgc atcttggtat actgcataaa gagctggaac accaaatcct
     8521 tcttcgtaag tacgacgtac caagtgtccc ggtcctttag gagcacacat gaagacatct
     8581 acatccgcag gaactttgat aaattcaaag tggatgttga aaccatgggc aaatccaact
     8641 gcgtttccag cttccaagtt tggagcgatt tctgcttcgt acaattcttg ttgaatttcg
     8701 tctggcgcca agatcatgat aacatcagcc aacttagtag cttctactac tgtgtaagta
     8761 tcaaatccat cttcttttgc tttatcaaaa gatttacctg gacgtacacc gataataacg
     8821 tcacgacctg aatcacgcaa gttttgagca tgcgcatgtc cttgtgaacc ataaccgata
     8881 acggcgattt ttttaccgtc aagtgctgct actttaacat ctttttcata ttccatttga
     8941 actgtcatag cttttctctc ttttctatta tttattgcct tttaggctgg tttaataaat
     9001 ttaagttgga tttttaatcg cgggtaaatc cagttgcacc cgttcgagca atattgcgaa
     9061 taccgtatgg gcgaatgact cgcaataggg cttcgctctt ttctgcattt cccgtcatct
     9121 gaatggtaat cgagcttggc gctacgtcta ccactgttgc acggaaaggt tgaataatcg
     9181 ctaaaatctc agctctcttc tcagctggcg ctgacatctt aaccaaaatc acctcgcgct
     9241 ccaaatgagg cttgtctgta atatctcgaa tgcgaatcac atcaatctga cgattgagct
     9301 gtttgatgat ttgctccact tcatcatgag aagcaacatc aataataata gtgatacgcg
     9361 atacattcgg atcttctgtt gctccaacag agatgctttc aatattaacc tgacgacgag
     9421 ataggacacc tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca
     9481 ttctacgcat ggaactgcac ccccaacatc tcatgattac tcttaccagc cggtaccatt
     9541 ggtaacacct gttccttacg agaaatatct acctcaatta gcataggaac atcctcagtg
     9601 atgacttcaa ggtcttgagc caaggtctca ggattgtcaa acttatagtt tttaatacca
     9661 taagcctgcg ccatcaattg gaaatcagga agggtatcaa agaccgactc tgatgttctg
     9721 ccttcataga aggattcctg ccactggcga accattccaa gtgaatgatt gttcagcata
     9781 accaccttga ttggcacctt gtaaatattc aaaatagcca actcctggtt ggtcatttgg
     9841 aaaccaccat ccccaacaaa caagactact tccttatctg ggttagcaat tttagcaccg
     9901 attgctgctg gaattccaaa gcccattgtt cccaaaccac ctgaagtcac taactgacgc
     9961 tcattttggt agggataata ctgagctgtc cacatttggt gttgaccaac gtctgttacc
    10021 acaatggcat ctccattcgt caattcacca attcgttcaa taactgcttg cggttgaacc
    10081 acacgctctt tcttgtcata agaacgaaca cgattcttgt ctttagtgac tttctcaatc
    10141 cacttttcag tattgttgtg aactgttggt tctgctagca acatttgcaa ggccttctta
    10201 gcatctccaa ctacaggaat gtctgcactg ataatcttgc caatctcagc tgggtcaata
    10261 tcaatgtggg caaccttagc attcttagcg aaagtcttag gattccccgt caaacggtca
    10321 tcgaaacgag aaccaatact aatcataaag tccgcttccg tcatggcaat atttgctgcg
    10381 aatgacccgt gcatgcctcc cattccaaga aagagtgggt gactcgttgc aatcgttcct
    10441 tgtcccaaaa gactggttac cactggaatt tgatagcgtt ctgcaaattc atttagttcc
    10501 gtagcagcct cagcataact aattccacca ccagctaaca agactggctt tttagccttg
    10561 gacaattgct tcaagatttt cttgatttgc atatcattcg gctcaagagt cggctgataa
    10621 cttggtaaat tcacttctgg tgaataaatg aagtctgttt ctaaagcaga tatgtctttt
    10681 ggtaggtcaa ttacaactgg ccctggacgg cctgtagttg cgatatggac agcttccgta
    10741 atgatacgcg gaatatcagc tgtctcacga acttggtaat tgtacttagt gattggcatg
    10801 gtaattccta cgatgtctgc ctcctgaaag gcatccttcc caatccctgc tcgcgccacc
    10861 tgacctgtaa agaccaaaag gggaacgcta tcgctcatag catccgcaat ccctgtaatg
    10921 gcatttgttg ctcctggtcc actagtgacg acggcaacac ccaactttcc agttgatttg
    10981 gcataacctt cagcttcatg caaacaacct tgctcatggc gccctagaat gtggcgaatg
    11041 cctttaaaat tatatatcgc atcataaaaa ggcaaaaccg caccaccagg ataaccaaag
    11101 atggtatcaa ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga
    11161 gattctaaac tgattttctc cattgttccc ctttctttcc tcttaaaaat aacttgttac
    11221 tatcatacca ttttttcaaa atttttcaag ataaaagaag aaattttctg aattttctat
    11281 tttaacgttt atttatgaat gttatttttg tttttattaa aaagataccg atttcattta
    11341 ctaaaaaaga aaaaagaact gatttctcag tccttcatta atcttattcc acactaaata
    11401 ggtatgggta aacaggttgt tgaccttggt gaatctcgac ttcaacgtct tcgaattctt
    11461 ctacgatttc ttgagcgatt tcattggcaa gttcttcgct tccgtcttca ccgacataga
    11521 aggttacgat ttcactatct tcatccaaca tatgtttcaa ggtttcagtc aatgtttggt
    11581 gcatatcagg gtttgacaca agaatttttc catccaccat acctagatta tcgttttcat
    11641 ggatttctaa gccatcgatc gttgtatcac gcacggctgt tgtgacgctt ccgctaacga
    11701 catcgctaag agcagctgtc atacgctctt ggttttcttc aatggacttg cttggatcaa
    11761 aggcaagaag acttgtcata ccttgaggaa gagtgcgagc ctctaccact actgctggtt
    11821 gctccaaaac ttctgccgca gattgagctg ccatgaagat gttcttgttg tttggcaaga
    11881 agatgatgtt acgggcattg acctgttcaa cagccttgat aaagtcttct gttgaagggt
    11941 tcatggtttg accgccttcg ataacataat ccacgccttg agaacagaag atatctgcta
    12001 gacctttacc agccaccaca gcaatcaaag catactcttt ttcttcagcc gacttgataa
    12061 cttgagtagc ttctttctca acctgtgctt cgtgttggtt acgcatattg tcaactttta
    12121 ccttgaccaa gctaccatat ttgagacctt cttgcataac aagtcctgga tcttctgtat
    12181 gaacatggac tttgacaatt tcatcatcgt taacaacgag gagagaatcc ccaagctcat
    12241 ccaagtagtt acggaattca tcgtagtcaa aatctttagc ataggttgga ccttgcttaa
    12301 gagctaccat gatttcagta cagtaaccaa acgtgatgtc ctcagtcgct acgtgaccag
    12361 ctacagactt atgatgctct acattgatca tctcactcat gttggcagga gtcgctacaa
    12421 agtcctcaga tgcaatatat tcgccagtaa gggctgaaag gaaaccttcg tagatgaaga
    12481 ccaatccttg accacctgag tccacaacgc caacttcttt caatactgga agcatgtctg
    12541 gtgttttagc tagagctgtt ttagcacctt ccaaggctgc gcgcatgact tcaacagtgt
    12601 catctgtttg ctcagctttt ttcttagcac cgatagcagc tccacgagaa actgtcaaaa
    12661 tcgttccttc aacaggtttc atcactgcct tataggcaac ttccacacct gattggaagg
    12721 ccagagccaa gtcttgacct gttaactcgt ctttatcctt gatagcttgg gaaaatccac
    12781 ggaaaagctg agacgtaatc actcctgagt tcccacgcgc acccatcaaa agccctttgg
    12841 caagaatgct cgctacttct ccaactgtag aagctggctt gtctgcaact tctttagcac
    12901 cattttcaat ggtcattccc atatttgtcc cagtatctcc atctggaact ggaaagacgt
    12961 ttaatgaatt gacatattca gcttgcttat tcaagcgagt tgatgcagcc tgcaccattt
    13021 cttgaaataa gctagtagta atttttgaca cggttattct cctacaactt tgatattttg
    13081 aatgtagaca tttacagtct gagcagtaat tccaagctgg ttttccaagc taaaggcaac
    13141 acgctcttga atgttttttg acacttcact aatctttgtt ccgtagctta acacggtata
    13201 tacatcaact gcaatactgc catcttcggc tgcctttacg acgacacctt tagaataatt
    13261 ttccttacct agcagggctt ggaaattatc tttgagggca tttttactag ccataccgac
    13321 cacaccaaaa atctcagttg ctgctccacc tacgacggtt gcaatcactt catctgttag
    13381 ttcgatttga ccatcttttg tattaatttt tacagtcatc ctttttacct caactagttg
    13441 atactctatt ttatcatatt tcagcccaag tgtaaaagca gaatgatgta tcagcggata
    13501 tttactctat ttttcaaacg atgttatacc cacaataaaa gaaaaaagac cctaaggtct
    13561 cctttgcttt tattattaaa cgcgttcaac tttacctgat ttcaaagcac gagctgaagc
    13621 ccaaactttt ttaggtttac catcgataag aacagtaact ttttgaaggt ttggttttac
    13681 ggcacgtttt gtttggttca tcgcgtgtga acggttgttt cctgatacag tcttacgacc
    13741 tgtaaagtaa catactttag ccattgtgtt ttcctcctat tagatctaat atagcggatg
    13801 tgctagcacc acataccgta ctatgttatc acactttctt gttttttgca agggaattgg
    13861 aagatttttt atttgtgtct taaatcaggt cttgcgtgac attttctgct ctccacatgc
    13921 catcgttgat taacagaaca ccagaattaa aattatgtgt ataaaaatca tctctaactg
    13981 cagctaaggg tatagccgtc aagtccaaat cccacagctc atctatcgat tttcttacaa
    14041 caatatctga atccaaatac agtacacgag actcgcttac atactttgga ataaaatacc
    14101 taaaaaagcc gcatatgaaa gtccctcaaa ggggagacga taacctttca gaatactact
    14161 gtcaatctaa acattcacaa tctcactatt caaagtctct agtctttttt ccattaattg
    14221 gaaccattct cgcggaaggt catcattaaa aacataaaac ttaagattat aatgatgaac
    14281 acaaagagat tttattgttg tttcaacttt atccatataa gcattatctg cacctaagac
    14341 aatcgctttt ttctcttctt tcacttttta tctcatttct ttttattccc atcatatgtt
    14401 tctacgtaac cattattttc gcctattcgt tcgtaaaacc ataccagtgg agattttaga
    14461 tgaagtccca ttacggttta caatttttac attacgacac agagttttac aaatcgattt
    14521 catttgccaa acgtagttag taaggcagtt agctaggcta cctactttct gctacgacgg
    14581 aaaaatccac aaatcaatac cattcgtgga tttttcctag tgaagcgttt aggtaagccg
    14641 ccatagcggt taccgattat tagattacga cacggagttt ggcaaatcga ttctatttgc
    14701 caaacgtagt tagtgaggca gttagctagt tcgccaaata gcgactagcg tccaacaatt
    14761 tggaacttta gttccaattg ttggtactga gtcacatctt ctcctctaac tctacgtctg
    14821 gatacttgtc cgcaaaccag cggagggcaa agtcattttc aaagagaaag actggttggt
    14881 caaaacggtc tttggctaag atattgcgac ttgacgacat ccgttcatcc aagtcctcag
    14941 gcttgatcca acgaacggtc tttttaccca ttgggctcat gaccacttcc gcattgtatt
    15001 cgccttccat acggtgttta aagacttcaa actggagttg accaacagct cccagcatgt
    15061 actcacctgt ttggtaattc ttataaagct gaacggctcc ttcttgcacc aattgctcaa
    15121 tccccttgtg gaaggatttt tgcttcataa cattcttagc agaaactttc atgaaaattt
    15181 caggagtaaa ggttggcaat ggttcaaatt caaacttgtt ttttccaacc gtcaaggtat
    15241 ccccaacctg ataagtaccg gtatcgtaaa ccccgataat atcacctgcc acggcattgg
    15301 tcacattctc acgactctcc gccataaact gggtaacatt agatagttta gcccccttac
    15361 cagtacgagg gagattgaca ctcatgccgc gctcaaattc gcctgatacg atacggacaa
    15421 aggcaatacg gtcacggtga cgaggatcca tgttggcttg gattttaaag acaaagcctg
    15481 agaaatcctt gtcataagga tccacaattt caccatctgt tttcttgtga ccatgtggtt
    15541 ctggagcaaa cttgaggaag atttcaagga aggtctgcac accaaagttt gtcagggctg
    15601 aaccgaaaaa gacaggcgtc aattctccag ccagaatagc ttcctctgaa aactcattcc
    15661 cagcttcatt taaaagctca atgtcatcct tgacttgctc gtagaaagga ttgctaccaa
    15721 aaagtttgtc tccatcttct aggctagcaa aacgctcatc ccctttataa agttctaaac
    15781 gttggttata gaggtcatac aagccctcaa aggctttccc catcccgata ggccagttca
    15841 tagggtagct agcaatgccc aagatttctt ccaattcttg caagagttcc aaaggctcac
    15901 gaccgtcacg gtccagcttg ttcataaagg taaagactgg aatgccacga tgtttcacaa
    15961 cctcaaacaa tttcttggtt tgagcctcga tccccttggc agagtccacg accatgaccg
    16021 cagcatccac cgccatcaag gtacgatagg tatcttctga gaagtcctcg tgccctggcg
    16081 tgtctaagat attcacgcgc ttgccgtcgt agtcaaattg cataacagat gaagtaacag
    16141 aaatcccacg ttgcttctcg atatccatcc agtcagattt agcaaaagtc cctgttttct
    16201 tcccttttac cgtaccagcc tcacgaatct cacccccaaa gtagagtaac tgctcagtga
    16261 tggttgtttt ccccgcgtcc gggtgggaga taatggcaaa ggtacgacgt ttcttaattt
    16321 cttcttgaat attcataagt tctctttctt tgattctcta tttttcttgt ttcaatagct
    16381 gagaatgatt tttacattgg attttaccat tcctttcaat actccattat atcggatttt
    16441 agcatttttt tcaatttcta tttcttttca cttccccctc ccttatttat aggaaaatat
    16501 ggtaaaatag aacagactaa aaatcatcat ttcacgaaag gatgcaagat gaaaattacg
    16561 caagaagagg taacacacgt tgccaatctt tcaaaattaa gattctctga agaagaaact
    16621 gctgcctttg cgaccacctt gtctaagatt gttgacatgg ttgaattgct gggcgaagtt
    16681 gacacaactg gtgtcgcacc tactacgact atggctgacc gcaagactgt actccgccct
    16741 gatgtggccg aagaaggaat agaccgtgat cgcttgttta aaaacgtacc tgaaaaagac
    16801 aactactata tcaaggtgcc agctatccta gacaatggag gagatgccta atgactttta
    16861 acaataaaac tattgaagag ttgcacaatc tccttgtctc taaggaaatt tctgcaacag
    16921 aattgaccca agcaacactt gaaaatatca agtctcgtga ggaagccctc aattcatttg
    16981 tcaccatcgc tgaggagcaa gctcttgttc aagctaaagc cattgatgaa gctggaattg
    17041 atgctgacaa tgtcctttca ggaattccac ttgctgttaa ggataacatc tctacagacg
    17101 gtattctcac aactgctgcc tcaaaaatgc tctacaacta tgagccaatc tttgatgcga
    17161 cagctgttgc caatgcaaaa accaagggca tgattgtcgt tggaaagacc aacatggacg
    17221 aatttgctat gggtggttca ggtgaaactt cacactacgg agcaactaaa aacgcttggg
    17281 accacagcaa ggttcctggt gggtcatcaa gtggttctgc cgcagctgta gcctcaggac
    17341 aagttcgctt gtcacttggt tctgatactg gtggttccat ccgccaacct gctgccttca
    17401 acggaatcgt tggtctcaaa ccaacctacg gaacagtttc acgtttcggt ctcattgcct
    17461 ttggcagctc attagaccag attggacctt ttgctcctac tgttaaggaa aatgccctct
    17521 tgctcaacgc tattgccagc gaagatgcta aagactctac ttctgctcat gtccgcatcg
    17581 ccgactttac ttcaaaaatc ggccaagaca tcaagggtat gaaaatcgct ttgcctaagg
    17641 aatacctcgg tgaaggaatt aacccagagg ttaaggaaac cattcaaagt gccgctaaac
    17701 actttgaaaa acttggtgct attgtcgaag aagtcagcct tcctcactct aaatacggag
    17761 ttgccgtata ctacatcatc gcttcatcag aagcttcatc aaacttgcaa cgctttgacg
    17821 gtatccgtta cggctatcgc gcagaagatg caaccaacct tgatgaaatc tatgtaaaca
    17881 gccgaagcca aggctttggt gaagaggtaa aacgtcgtat catgctgggt actttcagtc
    17941 tttcatcagg ttactatgat gcctactaca aaaaggctgg tcaagtccgt accctcatca
    18001 ttcaagattt cgaaaaagtc ttcgcggatt acgatttgat tttgggtcca actgctccaa
    18061 gtgttgccta tgacttggat tctctcaacc atgacccagt tgccatgtac ttagccgacc
    18121 tattgaccat acctgtaaac ttggcaggac tgcctggaat ttcgattcct gctggattct
    18181 ctcaaggtct acctgtcgga ctccaattga ttggtcccaa gtactctgag gaaaccattt
    18241 accaagctgc tgctgctttt gaagcaacaa cagactacca caaacaacaa cccgtgattt
    18301 ttggaggtga caactaatga actttgaaac agtcatcgga cttgaagtcc acgtagagct
    18361 caacaccaat tcaaaaatct tctcacctac ttctgcccac tttggaaatg accaaaatgc
    18421 caacactaac gtgattgact ggtctttccc aggagttcta ccagttctca ataaaggggt
    18481 tgttgatgcc ggtatcaagg ctgctcttgc cctcaacatg gacatccaca aaaagatgca
    18541 ctttgaccgc aagaactact tctatcctga taaccccaaa gcctaccaaa tttctcaatt
    18601 tgatgaacca atcggatata atggctggat tgaagtcaaa ctagaagacg gtacgaccaa
    18661 gaaaatcggt atcgaacgtg cccacctaga ggaagacgct ggtaaaaaca cccatggtac
    18721 agatggctac tcttatgttg acctcaaccg ccaaggggtt cccttgattg agattgtatc
    18781 tgaggcagat atgcgttctc ctgaagaagc ctatgcttat ctgacagccc tcaaggaagt
    18841 tatccagtac gctggcattt ctgacgttaa gatggaggaa ggttcgatgc gtgtggatgc
    18901 caacatctcc cttcgtcctt atggtcaaga gaaattcggt accaagactg aattgaagaa
    18961 cctcaactcc ttctcaaacg ttcgcaaggg gcttgaatac gaaatccaac gtcaagctga
    19021 aatacttcgc tcaggtggtc aaattcgcca agaaacacgc cgttacgatg aagctaacaa
    19081 aacaaccatc ctcatgcgtg tcaaggaagg ggctgcagac taccgctact tcccagaacc
    19141 agacctaccc ctctttgaaa tttctgacga gtggattgag gaaatgcgga ctgagttgcc
    19201 agagtttcca aaagaacgtc gtgcgcgtta tgtatctgac cttggtttat cagactacga
    19261 tgctagtcag ttgactgcta ataaagtcac ttctgatttc tttgaaaaag ctgttgccct
    19321 aggtggtgat gccaaacaag tctctaactg gctccaaggg gaaatcgctc agttcttgaa
    19381 tgctgaaggt aaaacactgg aacaaatcga attgacacca gaaaacttgg ttgaaatgat
    19441 tgccatcatc gaagacggta ctatttcatc taagattgcc aagaaagtct ttgtccacct
    19501 agctaaaaat ggcggtggcg cgcgtgaata cgtggaaaaa gcaggtatgg ttcaaatttc
    19561 agatccagct atcttgatcc caatcatcca ccaagtcttt gccgataacg aagctgctgt
    19621 tgccgacttc aagtcaggca aacgtaacgc cgacaaggcc ttcacaggat tccttatgaa
    19681 ggcaaccaaa ggccaagcca acccacaagt tgcccttaaa ctacttgcac aggaattggc
    19741 gaagttgaaa gaaaactaga cagaacaaaa ccagccctaa ggttggtttt ttcttctcta
    19801 ccaactccca ataactattt tggctttatt tccagagtat tttatggtaa aatgaagagt
    19861 aataatattt attaaagagg taaaaacatg attgaagcaa gtaaattaaa agctggtatg
    19921 acctttgaaa cagctgacgg caaattgatt cgcgttttgg aagctagtca ccacaaacca
    19981 ggtaaaggaa acacgatcat gcgtatgaaa ttgcgtgatg tccgtactgg ttctacattt
    20041 gacacaagct accgtccaga ggaaaaattt gaacaagcta ttatcgagac tgtcccagct
    20101 caatacttgt acaaaatgga tgacacagca tacttcatga atacagaaac ttacgaccaa
    20161 tacgaaatcc ctgtagtcaa tgttgaaaac gaattgcttt acatccttga aaactctgat
    20221 gtgaaaatcc aattctacgg aactgaagtg atcggtgtca ccgttcctac tactgttgag
    20281 ttgacagttg ctgaaactca accatctatc aaaggtgcta ctgttacagg ttctggtaaa
    20341 ccagcaacga tggaaactgg acttgtcgta aacgttccag acttcatcga agcaggacaa
    20401 aaactcgtta tcaacactgc agaaggaact tacgtttctc gtgcctaatc tctagaaaga
    20461 ggtcattcta tgggaattga agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt
    20521 gaaaaaatca ttgctatcgc tactgcaaag gtagagggtg ttcactcttt ttcaaacaga
    20581 tcagtgtctg ataccctttc aaaactttca ctcggccgtg gcatttatct taaaaacgtg
    20641 gacgaagaac tcacagcaga tatctatctc taccttgagt acggagtaaa agttcctaag
    20701 gtagcggttg ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa
    20761 ctcgctgcta tcaatattca cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa
    20821 ttgaaagatc tatttgacga ggacttcctc aatgactagt ccactattag aatctagacg
    20881 ccaactccgt aaatgcgctt ttcaagctct catgagcctt gagttcggta cggatgtcga
    20941 aactgcttgt cgtttcgcct atactcatga tcgtgaagat acggatgtac aacttccagc
    21001 ctttttgata gacctcgttt ctggtgttca agctaaaaag gaagaactag ataagcaaat
    21061 cactcagcat ttaaaagcag gttggaccat tgaacgctta acgctcgtgg agagaaacct
    21121 ccttcgcttg ggagtctttg aaatcacttc atttgacact cctcagctgg ttgctgttaa
    21181 tgaagctatc gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg
    21241 actgctcagc cagtttgtaa cagaagaaca ataaaaagaa aaagtgtttg atagatatca
    21301 aacacttttt tctttgcctc ccactatcta aaaaaatgat aatagatata attgtaaaca
    21361 aaaatccaga taggttttgc atgattgaga aagttaaaaa aactatggca gagaatcgtt
    21421 aatctcagat tgtcggtaga acgataaaca agggcaaaaa agaaaccaat cagactataa
    21481 tataataaac taattggatc tctgtgagat agtatcaaat ggctaatccc aaagatgata
    21541 gcagatagga taacatccaa atagtacttg gactagggaa agaaggtatt cataaaatac
    21601 cctctatcaa gagtctcctc aaaaacagga ccgatgatta caggcaggac aaaagataag
    21661 atagtcgata aaaaggttgg ttgtccattt gaaaaaagca cggtaaaata ctcatcatga
    21721 atattcctat gattaatcaa atgagcatag cgtgcccaaa aattaccgag aatctgataa
    21781 accacataag ttgcaaataa gtagaagaca aatgaccagt tccagctctt tttctcaaag
    21841 ataaagagca tctttttctt ttttaacctc caaattatat agaaggaaac ttcccactaa
    21901 tcccattgtt aaaataagag aatagacatc agctcctaac cctaaaatga tcgtcacata
    21961 caatccaatt gtttgtggta aataggtaga tagtaaaata ataagcaaaa atattccaaa
    22021 ttgtcttagt ttttttgtgt ttctcatcgt acttttttga aagattaccc tgctcggaag
    22081 ccgtacttcc aagcatctat ataagaatta agtgcccctt gcctcatata gggagcaaat
    22141 tctctataat ataaccatct actatatcca tcttcccaaa cagcaagacc acctgaagtt
    22201 tgctccaagt cctcagttga aagaactgta aatgtatttg tacctgtcat tgcaagtacc
    22261 ttcttaaaat agattgttgt aggctcacat ttatagtata tttctttttt tgtctatttt
    22321 atagcccatc tcctcaactg gcaatttttc gacctgaatt acatttttcc ataaaaaatg
    22381 agacctttct agtctcattt agtcattctt agtattttct aaatcgttga tagcgttctt
    22441 ccagcaactc ttctagcggt ttttgtgaaa gtctagccag ctccgtttgg agttcttttt
    22501 tgacactctt aatcagttct ttactagaaa gtcctatttc agaaatcacc ttatccacca
    22561 cgtccatttc taacagttca tgcgaagtga ttttcatcag ttctgctgct tccatggcgc
    22621 gagtaccgtc cttccataaa atggaagcaa agccttctgg actgagaatg gcatagatag
    22681 aattttccag catccagaca cggtccgcga cagctagagc cagagccccg cctgaaccac
    22741 cttcaccgat aataatggcg ataataggaa ctttcaggtc actcatttcc atgagattgc
    22801 gagcgatagc ttccccttga ccacgttctt ctgctccgac accaggataa gcacctgctg
    22861 tattaataaa ggtcacaact ggacggccaa atttctcagc ctgtttcatc aaccgcagtg
    22921 cctttcggta gccttctgga tgtggttggc caaaattccg tttgaggttg tcttgcaaac
    22981 tcttgccttt ttggatacca acaactgtta cagcttggtc tccaagccaa ccaataccac
    23041 caacaactgc accatcatca cgaaaagaac ggtcaccatg taattggata aattcatcaa
    23101 aaatgcctgt cgcaaagtcc aaggttgtca agcgactctg ctcacgcgct tctctgacta
    23161 tttttgcaat attcatctag gactccctcc atgcaatctg actaggctag caatcgtatc
    23221 tggtaagtct cttcttttga caatagcatc cacaaagcca tgttctaata ggaattctgc
    23281 cttttggaaa tcctcaggca agctttcacg aaccgtattt tcaatcacac gacgcccagc
    23341 aaaaccaacc aagctctgtg gttcagccag aatgatatcg ccttccatag cgaaagaagc
    23401 tgtcacacca ccagtcgttg gatctgtcaa aatggtcagg taaaagagac cagcatttga
    23461 atgacgttta accgccgcag agatcttagc catctgcatg agactcatga ttccttcctg
    23521 catacgggct ccaccagagg ctgtgaatag gacaactggc aatttttcga cagtcgcata
    23581 ctcaaacaaa cgagtgattt tttcacctac aaccgtaccc atagaagcca tgataaagtt
    23641 agaatccata atcccaagag ccacagtctg acctttaata agagcagttc ctgtcacaac
    23701 ggcttcatgc agacctgttt tttcacgcat agatgccagt ttcttttggt aaccagggaa
    23761 atgcaaggga tccttgcttt caatccctgt aaacaattct ttgaaggttc ccatatcaat
    23821 cgtcaaagcc aagcgttctt gggcagaaat acgaaaggta tagctacagt gcggacagat
    23881 acgttcactt cccagatcct tctgatagat ggtatgctta cagcctggac actgggaaaa
    23941 taattcatct ggaacctctg gcttagcttg aggtttttcc ctaaccgaac gattgggatt
    24001 gattcgaata tacttatctt ttttactaaa tagagccatt gattcccctt ttcggtttaa
    24061 actcttaaag tcattttatt ctttttcttg atatttaggt aagaaggttt ccatcaagaa
    24121 ggaagtatca taatccccag caatgacatt gcgatctgaa atgaggtcaa gctggaaatc
    24181 tgcattggtc tgcactcctt caatctctaa ttcatagagg gcacgttgca ttttcatcaa
    24241 ggcgtcaaaa cgattttcgc cgtgtactat gattttggca atcatactat cataataagg
    24301 cggaatggta taacctggat aaactgctga atccacgcgc aagccaactc caccacttgg
    24361 cagatagaga ttagtaatct tacctggact tggagcaaag ttaaaggctg ggttttctgc
    24421 attgatacga cactcgatgg catgaccgcg taggacaata tcttcttgct taacagacaa
    24481 aggctgacct gccgcaatgc aaatctgttc cttaacgata tcaacacctg aaacaaactc
    24541 tgttactgga tgttctacct gaacacgagt attcatctcc atgaaataga aattgctact
    24601 tgcttcatca agaagaaatt caatggttcc tgcattctca tagccaacaa actctgccgc
    24661 tcgaacagca gcagcaccta tttcatgacg cagcgttttt ccgattgcaa tcgagggact
    24721 ttcttccaaa accttttggt tattcctttg aagagaacaa tcccgctcac ccaagtgaat
    24781 cacatgtcca tgctcatcac ctaggatttg aacctcaatg tgccgagctg gatagataac
    24841 ccgttctatg tacatggcac cattgccata attggccttg gcctcactag aggcagtttc
    24901 aaaggcagaa acgaggtcat ctggtttttc aaccttacga atccctttac cacctccacc
    24961 tgctgaagcc ttgagcataa caggatagcc aattttttca gcaacaatca aagcttcttc
    25021 agagttatgc acttctccat ctgaacctgg tataacaggc acacctgctt taatcatctg
    25081 agcacgcgca ttgatcttat cccccatcat atccataaca tgaccagatg gaccgataaa
    25141 cttgatacct acttcttcac acatggtcgc aaatttggaa ttttcactga gaaatccaaa
    25201 accagggtga atagcttctg cctcagtcaa gactgcagct gatagaactg cattaatatt
    25261 gagataagac tctgttgcct tgccaggacc aatacaaact gcttcatctg ccaaaagcgt
    25321 atgaagagct tccttatcag cagttgaata aaccgctacc gtcgcaatcc ccaattcacg
    25381 tgccgcacgg ataatacgaa ccgcaatttc accacgattg gcaattaaaa tttttcgaaa
    25441 catggagaac ctccttagtt cccaattgca aaagtaagga taccactggc tgcaagcttg
    25501 ccatccactt cagcctttgc ttcaaccaca gctatggtgc cacgacgttt tacaaaagtc
    25561 gctgtcataa ccaattggtc gcctggtaca acttgcttct tgaacttaac cttgtccata
    25621 ccagcgtaaa agaccagttt tcctttattt tcaggttttg ataactccaa cacaccagca
    25681 gtttgcgcca aggcttccat aatcagaaca cctggcataa ctgggtattg aggaaagtgg
    25741 ccgttaaaga aaggttcgtt aatggtcaca tttttgatag caacaatggt atcctcgctc
    25801 acttccaaga cacggtccac tagaagcata ggataacggt ggggaagagc ttctttgatt
    25861 ccttgaatat cgatcatttg atacgtacca atcctttacc aaactcaacc atttcttcgt
    25921 tagagacgag aatttccgtt accacaccat ccttaggagc tgggatttca ttcatgactt
    25981 tcatggcttc gataattacc aatgtttgac cttttttgac actatcacca actgtaacga
    26041 aggcaggttt atctggtcca gcagccaagt aaaccactcc aacaagtgga ctctctacaa
    26101 gatttccctc actagccaca cttgcttcag ctggagctgg aacttcttct gctacagtct
    26161 ctgctggagc agatgtagga gctactggac tcggtgttgc tagaacgggt gctggagcga
    26221 cttgagttgc aacttcaggc acaggtctcg cttcattctt gctaaactgc aactcatccg
    26281 tcccattttt ataagaaaat tctctcaaac ttgactggtc aaattgagtc atcaagtctt
    26341 taatatcgtt taaattcata cttatctatt ctcccaacgt ttgaaagcaa gaactgcatt
    26401 gtggcctcca aaaccaaaag tatttgaaat agcgtatgga atttctttct ccaagccttg
    26461 tccataaacg acattagctt cgatataatc tgatacttca cttgtcccag ctgtcattgg
    26521 tacaaagtta tgacgcatag cttcgatggt gacgatagct tctactgcac ccgcagcccc
    26581 cagcaaatgt cctgtaaaag acttggttga tgatacaggt acttccttac caagaacagc
    26641 tacgatagca ccactttctc ctttttcatt ggcaggagtt gacgttccgt gagcattgac
    26701 ataggctact tgctctggag aaatctcagc ttcttccaag gctagtttga tggccttgat
    26761 agctccctga ccttctggat gtggagaagt catgtggtag gcatcacaag tatttccgta
    26821 accaaccact tcagccagga tagtagctcc acgtttttca gcgtgttcaa gactttctag
    26881 aaccaacatc cctgaacctt cacccataac aaacccattg cgatccttat caaatgggat
    26941 cgaagcacga gttggatcct ctgtagtaga gagagctgtt aaggcttgga aaccagcgat
    27001 ggcaaaaggt gtgatagaag cttctgttcc tcccaccaac atcacatctt ggaaaccaaa
    27061 cttaatggag cggaaggcat ccccaatcgc atcatttgat gaagagcagg cagtattgat
    27121 agatttacaa acaccgtttg caccaaaacg catggctaca ttcccagaag ccatatttgg
    27181 taaagctttt ggaagagtca ttggtttgac acgtttgggt cctttttcat gaaggcgaag
    27241 tacctgatct tcaatttcct tgattccacc aataccagat gcaacgataa caccaaaacg
    27301 atccctatta agagcctcta catcaagatt ggcatgattt acagcctctt gggctgcata
    27361 caaggcatat aaagaatagt tatcaaaacg gttggtatct ttttttacaa agtatttatc
    27421 gaacggaaaa tcttggattt ctgccgcatt atgcacatca aagtcactat gatcaaattt
    27481 tgtaatgcca ccaatgccga ttttcccagt tgctaaacta ttccaaaatt cttctggtgt
    27541 atttccgatt ggagatgtta ctccataacc tgttaccact actcgattta gtttcattct
    27601 tttcacctct agctttcgct acatacttaa gccaccatca atggcaacca cttgaccagt
    27661 tagataatct tggcctgcta aaaatactgt caaatctgca acctgctctg cctgcccaaa
    27721 ttctttcatc ggaatctgag ctagtgtagc ttccttaatc ttatctgata agatagctgt
    27781 catatcagac tcaatcattc ctggagcaat cacattgact cgtatattcc gactagcgac
    27841 ctcgcgtgcc acagacttgg taaagccaat caagccagcc ttagaagcag catagttagc
    27901 ttgaccaata ttccccatca aaccaacaac actagacata ttaatgatag caccttctct
    27961 ggctttcatc atcggtttca agactgattg tgtcatatta aaggcaccag tcagattgac
    28021 cttgagcact ttttcaaaat ctgcttctgt catcttgagc ataagagtat cttgggtaat
    28081 ccctgcattg ttgaccaaaa catctactga acccagttct gcaatagctt gatcaatcat
    28141 acgcttagcg tctgcaaaat ctgatacatc tcctgaaatg ggaaccacct tgataccata
    28201 gtttgaaaac tcagcgagca attcttctga gattgcccca cgactgttta agacaatgtt
    28261 ggctcctgct tgagcaaact tgtgggcgat ggcaagacca attccacgac tcgaacctgt
    28321 aataaagata tttttatgtt ctagtttcat ttttttcctt tcaaaacttc tacttatttt
    28381 agtctatttt tctaaaagtg ctactaaact cgcttgatct tccacatgag ctaagtgagc
    28441 agtttgatca atttttttaa caaaacctga caagactttc cccggtccaa tctcgataaa
    28501 gttgcttatg cctgcttctt gcatgacccc aatactttca tagaaacgaa cgggttcctt
    28561 gacctgacgc gtcaagagct gagcaatgtc ctctttttgc atcacagcag cttctgtatt
    28621 gccgactagg ggacaagtaa aatctgaaaa acttacctga gctagagttt cagctagttt
    28681 ctggctagca ggctcaagga gagcggtgtg aaagggccct gacaccttaa gaggaatcaa
    28741 gcgtttggca cctgcttctt gcaaaagttc aaccgctcga tcaactgcaa ccacttctcc
    28801 agcaatgacg atttgtgcag gtgtgttata gttggctgga gtaaccactc caagttcaga
    28861 agctttttga caggcttctt caatgacctc tactggcgta ttgagaactg ctaccatctt
    28921 gccagagtca gcaggagccg cttcttccat ataggctcca cgcttagcta ccaaggcaac
    28981 cgcatcttca aaatccaagg cgccgcttgc caccaaggca gagtattctc caagagacaa
    29041 accagcaacc atatcaggct gatagccctt ttcttgcaat aaacggtaga tagcaaccga
    29101 agtcgctaga atggctggtt gcgtatagcg ggtctgattg agtttgtctt cttccgtatc
    29161 gatgagataa cgtaaatcat aaccgagcac ctgactcgct cgatcaatcg tttctttgac
    29221 aatcggatac tgatcataga aatcccgtcc catccctaga tactgggcac cttgaccagc
    29281 aaataaaaag gctgttttag tcatttctta caactcctgc ccagcgagag gcttcttctt
    29341 gaattttctt agcggctccg taatacaaat cttttaggat ttcttcagct gtttcttctt
    29401 tagaaacaag ccctgcgatt tgacctgcca tgacagagcc accatccaca tcaccgtgaa
    29461 caactgcttt ggctagggca cctgctccca tttgttcaaa gatttctaaa tcaggatctt
    29521 cctgcttaaa ggcatctttt tcagccagtt caaaatctct agtcaactga tttttaatag
    29581 cacgaacagc atgaccaaag tgctgagctg aaatcgtagt atcaatatcc cttgctttta
    29641 aaattttctc cttgtagttt ggatgggcat tcgactcttt tgcaactaca aaccgtgtcc
    29701 ccacctgtac agcctctgca cctagcataa agccagccgc agcaccttca ccatccgcaa
    29761 ttcctcctgc agcaataaca ggaatagata tagctgtggc tacctgtcgc accaaggtca
    29821 tggttgttaa tttaccgata tgccccccag cttccattcc ttctgcaata acagcgtctg
    29881 caccgatttt ttccatgcgt ttagctaaag cgacactagg aacaacagga ataacgatta
    29941 tcccagcttc atggaaacgt tccatatact tgcttggatt tcctgctcct gttgtgacaa
    30001 ctttaacacc ttcttcaata acgagatcca cgatatcttc cacaaaggga gataagagca
    30061 tgatgttgac cccaaagggt ttatcagtca atgatttgat tttatcaata ttggccttga
    30121 caacttcttt cggggcattt cccccaccga taattcctaa tcctccagcc ttggaaacag
    30181 cccctgccaa atcaccatca gcaacccagg ccatccctcc ttggaaaata ggatagtcaa
    30241 tcttcaataa ttctgtaata cgcgttttca tagtgcctcc aaccttcctt gcttacgtaa
    30301 tagttcgatt tcaccataat ttgacagtca aactattacc taaacaagag ggagtgggtt
    30361 tctccctact ccttctacta atattctgct tattttgctt gctcttcaac gtaagcaacc
    30421 aagtcaccaa ctgttttcaa gtcattttct gcttcgattt ggatatcaaa agcatcttcg
    30481 atttctgaga ttacttggaa caagtccaat gaatctgcgt ccaaatcatc aaaagttgat
    30541 tcaagtgtta cttctgatgc gtcttttcca agttcttcaa cgataatttc ttgtactttt
    30601 tcaaatactg ccatgatagg actcctttaa aataaatagt ttttttataa caatgtgttc
    30661 accacatgat tacctaaatt gtaagaatga gcgtgcccca ggtcaagcct ccaccgaagc
    30721 ctgatagaag aacagtctgg ctaccatcta aagggatgag accttgttct acacactctg
    30781 aaagtaaaat cgggatactg gctgcactgg tattgccata ttccatcata ttggctggaa
    30841 gtttggctcg gtcaacacca atttttctag ccatcttatc caaaatacgg tcattggctt
    30901 gatgaagtag cagataatcc aagtctgtca cctctatagg agattcatca atagtctgct
    30961 tgatagactt ggctacatct cgaatggcaa aatcaaagac tgtgcgtcca tccatcttca
    31021 aaaacgaatc tgcactttct tgatctgaaa atggagaatg caaacctgaa tgcccataag
    31081 ttaaacactc gctgcgactt ccatcgctat tgagactctc agctaagaaa tgctcttgct
    31141 cgctagcttc taacaagaca ccaccagcac catctccaaa caacacagct gttgatcgat
    31201 ccgaccaatc gactgcctta gagagggttt cactaccaat caccaagcct ttttgaaagc
    31261 gaccagaagc gataaacttt tcagcagttg aaagagcaaa tacaaatcca ctgcaagccg
    31321 cggttaagtc aaaagcaaag gctttattag cgccaatatt agcttgaaca cgagcagctg
    31381 tagagggcat catcgaatct ggagtaatgg tagctaggat gataaaatcc agttcttttc
    31441 ctgttattcc agcttttgcc atcagtttct tagcaacctc tgtagccaaa tcactggtag
    31501 attctgttct tgaaatatgc ctttgtcgta ttcccgttcg acttgaaatc cactcatcat
    31561 tggtatccat aatctgagcc aagtcgtgat ttgtaaccac ttgctctggc acataatgag
    31621 caacctgact tatttttgca aaagccatta tttcaaatcc tccaaaaatt ggtaaagatt
    31681 agtcaagcct ttacccatga cagcaatttc ttcctcgctc atgccatcaa taattttttc
    31741 taccatggcc ttgtggaagc gtttatgcag tctatgaatc aagcgaccct tctttgtcaa
    31801 atgcagatgc accacacgac gatcctgttc tgaccgaact cgctcaatgt agcccttacg
    31861 ctctaaattg ttcaaacttg tcgtaacagt tccaagagtt accatcaact cttttgacac
    31921 ttgacttgga gtcacatctg gagccttacc gatgacatca atcgtatgca tttctttgat
    31981 ggagatatcc ttaaaacgac tacctctcaa gttcacttcc tcaattacaa ggacattgtt
    32041 aaatatagat gttaaatatt cattgattcg ttggtagtcc atttttcata tccctccttc
    32101 ttcaaaaaac ttttacaatc aaaacatttg acagaaagaa ttatatcaaa attcaaagaa
    32161 tgatgcaagt attttttatt ttcctgtaaa tttaggtctt cttctctccg aatgagctcg
    32221 cactccttct ttgaaatcct ctgtttgagc taaggatttc tgtaggttca gttctaaagt
    32281 agcgtaaccc tgccagtctt taaactggct ctcccaaacc aacttcttaa tggcagcata
    32341 ggaattactt gaagcacgtc ttaatttttt aagaagctgt tctctcgtct tttcaagttt
    32401 ttcagcttca gaaacgcggt aaaccaaccc ccattctagg gctttttctg ccgttaaagc
    32461 ctctcctgtc atagctaatt gagcagcacg cgtcacaccg atactgcggc tcaagagatg
    32521 aattccccct gcatctggag ccaaaccaac accaacaaag gcttggataa acttagcctt
    32581 atccgtcgcc aaacagaaat ctgccgcaac agccatattc gctgcggcac ctgcaacagc
    32641 accgtcaact tccattaaaa caggtttggc tatttgcttg attttataag aaatcgtatt
    32701 gaccaactct gcgatttttg tcaatgatgg aatatcatcc tcatccactg cccgcttcat
    32761 ctctaccaaa tctcccccaa ctgagaagac ctttccattg gcattgatta agataaaatg
    32821 cacagctgga ttttcttctg ccaaagtcag agcttctaaa atctcctcac acatgggaat
    32881 atgaaaacca tttgcgacct caggacggtt caaggtaagg attgccaagt cctcttcaag
    32941 ctgataaata atgtgttcca tcagggctcc tttttatttt ataagcagtt tatactcttc
    33001 gaaaatctct ttaaaccgcg tcagcttcca tctgcaacct caaaacagta ttttgagctg
    33061 acttcggcta gcttcctagt ttgctctttg attttcattg agtgttaaat tatttcactg
    33121 tcaaagtata tcttttttag gagcagaaag caagaaaaaa tcgactgaaa gtgtctcagc
    33181 cgataagaaa ggctcgcttt aggctagaaa gcatcttatt ttcttcattg aaaaaagcct
    33241 ttctttctgc tataatcgta taaaatactt actttaggag ttcttatgaa agttgttaaa
    33301 tttggaggta gttctcttgc ctctgctagt caattagaaa aagttttaaa catcgtcaaa
    33361 agcgattcag agcgtcgttt tgtagtcgtt tctgcgcctg gtaaacgcaa tgctgaagat
    33421 actaaggtta cggatgccct gattaaatac taccgcgact atgttgcggg taacgatatt
    33481 agcaagagcc aaagctggat tatcgaccgc tatgctgcta tggttagtga attgggacta
    33541 aaaccagctg tgctagaaaa aatttctaaa agcattcacg ccttggccac tcttcctatt
    33601 gaagaaaatg aatttctcta cgatactttc ctagcagccg gtgaaaataa caatgccaaa
    33661 ttgattgctg cctactttaa ccaaaatggt atcgatgcac gctatatgca ccctagagaa
    33721 gctgggattg tggtcacaag tgaacctggt cacgctcgca tcattccatc aagttatgac
    33781 aagattgaag aattgacaaa caccaatgaa gtccttgtca ttcctggttt ctttggtgtc
    33841 actaaggaaa atcaaatctg tactttctca cgtggaggtt ctgatattac aggttctatc
    33901 attgctgctg gtgtcaaagc tgacctctat gaaaacttta cggacgttga tggtatcttt
    33961 gcagcccacc ctggtattat ccaccaacca cactcgattc ctgagttgac ctaccgtgaa
    34021 atgcgcgagt tggcctatgc aggcttctca gtccttcatg acgaggctct tcttcctgcc
    34081 taccgtggaa aaattcctct ggttatcaag aataccaaca accctgacca tccaggtact
    34141 cgtatcgttc taaaacacag taatgatgaa tttccagttg tgggaattgc tggtgactca
    34201 ggctttgtca gcattaacat gtcgaaatac ctcatgaacc gtgaggttgg atttggccgc
    34261 aaggttctgc aaatcctgga agaacttaac atcggttggg aacatatgcc aacaggtatc
    34321 gacgatcttt ctatcattct ccgttctcgc caactaactc ccatcaagga agaagaaatc
    34381 ctgcgtcagt tggttcaaaa ggctaaagta gatcatgcag aaatcgaaca cgacctttct
    34441 atcattatga ttgttggtga aaaaatgaag agccacatcg gagtgactgc tactgcgaca
    34501 cgcgctctat ctgaaaacaa aatcaacatc cagatgatgt ctcaaggttc tagtgaagtt
    34561 tctattatgt ttgttgtcaa taaagaccaa gagaaagcag caattaaagc cctctacaat
    34621 gccttttttg gagaaagtaa ggaagactaa gcatggccca atcacttaac aaaacagtac
    34681 tcctcagtac gacaggcact tcctacctct ctatcgctgg gaaagttggg aaattccttg
    34741 tcggagatca ggctttggaa ttttacccag atgtcaatgt cgaacaattt atccagattc
    34801 cttggagcca tatcaatcag attggagcca atgtcactgg atgcaaaatc agccgccact
    34861 tcgaagtctt tacagacaaa ggaaaattcc tctttgcttc aaaagactca ggtgccattc
    34921 tcaaaattgc acgcgaaaaa ctaggcaatg acaaggtcgt caaacttccg accctgattc
    34981 agacaattag ccaaaaattt aaaaatctat ttgcaaaaaa gtagaaactt tagtataatc
    35041 atagcaacgg ataagtaggt tatcttcttc tttcagaaag tctgcgggta ctgcgagcag
    35101 ataggaggga taggtgaaat tctaccgtta gtaacaatta catacacaat caagtgcaca
    35161 cctgtgaagt tggatggaac cgtggccctg ccactccaac gttttgtcag gcgtgctttt
    35221 ttcataaagg agttcttatg ttagatatca aacgtattcg tacagatttt gaagctgtcg
    35281 cagaaaaatt agctacacgt ggtgtagatg ctgctgtctt gaatgaaatg aaagaaatcg
    35341 atgctaaacg tcgtaacatc ttggtcaagg ttgaaactct caaagcagaa cgtaacacag
    35401 tttctgctga gattgcccaa gctaagcgca acaaggaaaa tacagatgac aagattgctg
    35461 ccatgcaaaa tctatctgct gaggttaaag ccttggatgc tgaattggca gaaatcgata
    35521 ctaaattgac agaatttaca acgactcttc caaatatccc agctgacagc gttcctgttg
    35581 gggctgacga agacgacaat gtggaagttc gccgttgggg tactccacgc gagtttgact
    35641 tcgaacctaa agctcactgg gatctcggtg aagaccttgg tatccttgac tgggaacgcg
    35701 gtggtaaggt aacaggcgct cgcttcctct tctataaagg cctcggtgct cgtttggaac
    35761 gtgctatcta caactttatg ttggatgaac atggaaaaga aggctatact gaagtcatca
    35821 caccttacat agtcaaccat gattctatgt ttggtactgg tcagtatcca aaatttaagg
    35881 aagatacttt tgaactcagc gataccaact ttgtcttgat tccaactgct gaagttcctc
    35941 tgacaaacta ctaccgtgat gaaatcttag acggcaaaga tcttccaatc tacttcactg
    36001 ctatgagtcc gtcattccgt tctgaggctg gttctgccgg tcgtgatacg cgtggcttga
    36061 tccgtttgca ccaattccac aaggttgaaa tggtcaaatt tgccaaacca gaagaatctt
    36121 acgaagaatt ggaaaaaatg acagccaacg ctgaaaacat tcttcaaaaa ctcaaccttc
    36181 cataccgtgt cgttgctctc tctactggag atatgggctt ctcagctgcg aagacttacg
    36241 acttggaagt gtggattcca gcacaaaaca attaccgtga aatctcaagc tgttcaaaca
    36301 cagaagattt ccaagcccgt cgtgcccaaa tccgttaccg tgatgaagca gatggcaagg
    36361 tgaaactcct tcataccttg aacggttctg gacttgcagt tggacgtaca gtggctgcaa
    36421 ttcttgaaaa ttaccaaaat gaagatggtt ctgtgaccat cccagaagca cttcgtccat
    36481 acatgggtgg agctgaagtc atcaaaccat aaaaaataag gtttagctat ttctagctag
    36541 accttttttc gtaaccaaat cagataagca cctagtacaa agaataaaat agttaggcat
    36601 ataatggttt cagccaatac caggtaatcc agaaatggaa gtttcaaaat tccctgagcc
    36661 atcttgagcg aggtcgctgt gataatggtt gggaaggtga gggctgagaa ggctggttga
    36721 aaaccttgtt ttaaaatgtt gggcagacga gttaaaacaa agaaaaagaa ggattgagaa
    36781 gccaaaatca tgacaatcaa gacccaagtc ggcaggctgg ttcctcctac tcgaactaga
    36841 gaagccaaga gtagagagaa aggagcacag tagattcctt cttgtccaag caaggctagt
    36901 gggagtggat gtttctttaa atcgctataa ataaggggat agagatagaa ggtcaagaga
    36961 aaaccaaaac tcaaggtcgc ataggcaatt tcgataatac ctaccagagg ataggtcaag
    37021 gcagccactg ctatccccac atagagaacc gtccagcttg gagtggcatg aaccctccgc
    37081 cctggacaag caaacttgat ggtaaaacca gcaatcaagg tcaaatccaa gagaaatgaa
    37141 aaccaccaaa tcccttgtgc taccaaagga agataagaga atacgcgaaa gacataggtc
    37201 gataaaatca tcccagccat aggaaaggtt gccattcctg acaaaagagg gggcttggtc
    37261 aattcttgct tggtttcttt ccaattaaag agatgcagaa ttagaaagta aatccataaa
    37321 accaaaccag tcagactaaa aagatgggat agaaccggca acgtatctaa aataagattt
    37381 ccagctcctg ccaaacctag caaacaacct gaaaatacta aggggagttt tttcatccta
    37441 acctccaata atcatgttag tttcagtata acataaaagc gcttaaatga ggatttaaaa
    37501 aaacgaagtc cgcttatttc agacttcatt ttactcagat atgaattagg cataaggttg
    37561 caattctgga ttaattggtg tattagctaa gttgttggca tagttacaga ggattgctag
    37621 gctgacacca aaaaccacat ccaaggcatt ttgttgagtg tagccagctt ctaaaaactc
    37681 agacaaggct tcatctccta cacgaccctt ggtattgata actgccaagg taaacttagc
    37741 tagggtatcc aatttaggat ctgtttcaat tggagtacga ttgcgaagag cttgaatcaa
    37801 gtcatcattc atctggattt gtttgatgga aaaggctgtg tgacctgcga cacagaaggc
    37861 acaaccattg gtcacggctg ccgtgatttg caccacttca cgctcaacgg gtgtcaggct
    37921 gttgcgacgg tggatagatg agacaatttg gtaggcttct aaaacagtcg gggcattggc
    37981 caagagaccg attaggttgg gaatatagcc attgttgtct ttttctactg tttcaagaat
    38041 ttctttcact tctgctggtg ctgactctac tgtatggata gtaaatgttg tcataagata
    38101 cctcttttct tattattgac actaatatta ttggaaaatc ttataaaatc ctgattccta
    38161 agtttatcta agataaagct ttattctctc ataagatttt cgttgttata ttagtttatc
    38221 acacttccaa tcacttgcat aatatatatt atatatcagg ctgataaaaa ttatttatag
    38281 gcaaaaaaat cacacgagct gtgtgattcc attatttgtc aaaatacttt ttagtttcag
    38341 caataacgac tggcgacaag accaagaggg caatcaagtt tggcagagcc atcaaggcgt
    38401 taacgatatc tgcgataatc cagaccatat ccaactcgat aaatcctcct aacaagacca
    38461 tgagcacaaa aaccacacgg tagagccaga taaagcgaac cccaaagagg aactcaaaac
    38521 agcgttctcc gtaatagttc caacctagaa tcgttgtaaa ggcaaaaagt acaaggaaga
    38581 tggtcaagag ggcaggccca aagtgtgaaa agactgttga gaaagctgac tgagtcaagg
    38641 caaccccatt caagtcacca ctccaaactc cagttaccaa gatggtcaaa ccagttagag
    38701 tacaaatgat gagggtatca ataaaggttc ctgtcatgga aatcaaacct tgctctactg
    38761 gttcatttgt cttggcagct gcagctgcaa taggagcaga acccagacca gattcgtttg
    38821 agaacacacc acgcgccaca ccattttgaa tagccatccg aacgctagca ccagcaaatc
    38881 cacctaccgc agcaagggga ctaaaagctg aggtaaagac taaagcgatt gtgccaggga
    38941 tttttccaat attaaagaaa ataactgtaa gagttcctaa gatataaatg atggccataa
    39001 aaggaacaac agtagttgaa accttagaaa tagacttgag tccaccaaag actgcaatcg
    39061 ctacaaagac agacaagacg agagctgtga tggctggcga aatcgtcgtt gtattttgga
    39121 tagattctgc aatcgagttg acttgggtga aggttccgat tcccaagaga gcaaccaata
    39181 ctcctgctac tgcaaacaaa acagcaagtg gtcgccactt ttctcccatc cctagaagga
    39241 tataatgcat gggacctccc gctactgcac catggtcgtc cttggtgcgg tatttgatgg
    39301 ccaagagtcc ttccgcatac ttggtagcca ttccaaagaa agccgccatc cacatccaaa
    39361 atagagctcc tggtccacca accttgatag ccgtcgcaac tcctatgata tttcctgttc
    39421 caacagttga tgccaaggct gtacacagag ctgcaaaact ggatacatca ccatgtccct
    39481 tatcctggat aaaaataagc tgaaaagcct tgggcagacg caaaacctgc aagagtccta
    39541 gccgaatagt taggtaaatc cctgttccga ccaataaaat caagaggggc ggtccccaag
    39601 caaaagcatc gattgattta agcaattcta acatttcctt ctcctatcgt ttcaacccca
    39661 aaagaaagag cacatgcaag atacatgtac tctggaatgc ttagataaat gctaaaaagc
    39721 ggtctatcct agctctgtcc ttttacctga gagtttgagc agttgcctgc cttgcccctt
    39781 cggtgccttt acggtctctc cagagttccg tccatttaca gtcatggaaa atcaaacgat
    39841 tccccacttc tattaaactt cattcggtgt tggtatttaa ttgattctaa ttttacaaaa
    39901 aatgttggct tttgtcaatg tgtttattag taaaaattag ttcaacagtt tttactttat
    39961 aaagtccaga atactgctat cctttaaaag tgacaatagt cgcaccactg cctccagcat
    40021 tttgtggggc atagccgaaa ctcttgacat gtttgtttct ttgcaagtat ttggtaactc
    40081 cttcacggat gactcctgtt ccgataccat ggatgatatc aacttgagcc atattgttaa
    40141 gcaaggcttg gtcgatgaag atatctagct cattcatggc ttcttcatag cgcttgcctc
    40201 gaagatccag tctagcttga ggtcctcgcc cagaagttcg tttcacaaca ttgacctgct
    40261 tcttcttgac tggtttttct tgctgggctt gaacaagatc aaactctttc tcttccaagg
    40321 tcatcttaat caagccaact tgggcttccc agcggccgtc cttgagttga ctggtcaagg
    40381 taccacgctg accataactg agaaccacga tatcatctcc cacctttgga gctcgttttt
    40441 tcttggcctt ttgaaggacc ttgtttttag acaagtccac tttttcagga gccaattttt
    40501 tcaacttggc cttggcttca atgatttcgt ggggcttgag ttgggattta ctgtggagat
    40561 ttttgagaat ctggtcactt tcacttaggg ccatatccac aatctcagca gcctgttcac
    40621 gcgccttgtt aagctcggtt tccttttcac gattaagctc gttgtagagt ttttttagcg
    40681 cacggttcat cttgagattt tcttgctcca cctcacggat attgtccaaa cgtttgcggc
    40741 tttccagcgt ctgctcttct aattgctcaa tgatacgatt gacgtcattg tcctgatcga
    40801 tctgctgact ggcatctcct acgataactt cagataggcc tagacgtttg gcaatttcaa
    40861 aggcattact tcggccagga acaccctgca taaagcgata ggtcgggcga agagttgcag
    40921 tatcaaactc catactggca ttttgcacaa aggctgtctc aataccgtag gccttgagtt
    40981 ctggatagtg ggtcgtcgcc atggtcttga tttgacgcag gcgaaggtcc tccagaatag
    41041 ccatggcaag ggctgctccc tcttggggat cagtaccagc ccccagctca tccaaaagta
    41101 agagtgaatg ttggttgacc ttgccaagaa tatccacgat attggtcata tggctagaga
    41161 aggtagacaa gctctgctca atagactgct catctccaat atcagcaaag atttcttcaa
    41221 aaataccaac acgacttccc ttgtctgcta aaatcggcaa tcctgactgg gccatgacct
    41281 gtgtcaagcc cagagtttta agcatgatgg tcttcccacc tgtattggga cctgtaatga
    41341 caatagctgt taaatcttga ccaaaatgga catcatttgc gacggcattt ttgaccaaag
    41401 gatggcagac atggagcagt tgaatctctt gattttctga cagctgaggc acgactgctt
    41461 gtctttcttg gataaatcga accttggcac gaatcaagtc cagatgaccg ataatccaag
    41521 cgtcattagc aatctcagcc gcatgagggc ggacacgctc agaaatttct tggagaatgc
    41581 gaagcatttc atagcgctca tctgctcgca gactagcaat ttcttcgctc agtttgacta
    41641 cctcacgtgg ttcgatatag acggtgtttc cactagcaga aatatcatga acgacacctg
    41701 caatcttatt gcggtaggtg tttttgactg gtaaaacctg acggccattt ctgctagcaa
    41761 caattccttc tgtcaacatc tgcgcttttt gcttgagcaa gtcttgtaaa acatcgcgta
    41821 cctgactctc gctatcatgt atttttcgac ggattcgcgc caatttttca ctggcaaaat
    41881 tttcaatgaa acccgcatca ttaaaggcct gaagatttcc ttgtaattgc ggaaaatcat
    41941 gtaatttctc aaaccaaagg gctaattctt ccaagctgac attttccaga ttggcgtaaa
    42001 aactttgaag ttctcggctg gcaagaagca cgcgtttcaa gagtaggaac tcctcgatat
    42061 tgagatccgc tcccatctcc aacctcttgc agactcctgc aatttcctta gttgagagaa
    42121 tagtaaaatg cggttgctcg acgaaaagag cctgcatttc cttcatctca gcaaaagcct
    42181 gtttgatttt atctgctttg gcagtcggag ccagttgtct caattgctcc aagccctgct
    42241 cggtcaacaa atgaggctca aacaaggcct tgaccttatc gaactctaat gtttctaata
    42301 ttttcttatt catctttatt tccttgtctt tgaatgtcta ggtgtggtag ctttttacat
    42361 tctgatagat tcgagtcaaa tctgtaaaca aagggctagg aaaactcctg ccctttttat
    42421 ccgattaaat ttgtcaccca gatttgtttg agccaactgg ttgttatcgg tatgctctgg
    42481 atgatgtgtt ttgcgacgat actcttttca agaggatttt gtataactgc catggggatg
    42541 gtcgccaaga ttgtcaaggc catttgcaag acaaataagg tcaccaacat ggacaagata
    42601 cctgctgaaa cttggaacaa cttaccaccc agttttttgc taggaatcaa gtgtaagaga
    42661 agaccaagca aacgaccaat gctatagaca atcccaaata caagcaagta gccgatgcct
    42721 gcataaaaga ccttatccag ctgaaagagt tgatccgatg ggaaaaagaa agtcccctga
    42781 ccttcctgcg aatttgcata agggagcaat aaatggaatt gctctccaag ccccttataa
    42841 aactggccag ccataaaagc cgatgccatg gctgaaatca ggtaataaac ctgtaagagc
    42901 aggcctctcc gatagccgat ataaaatccc caaaccaaga ccaatagaag aaggaatgaa
    42961 atcataagga atcctcaatc ttgctctgtt cttgcttaca agtcacaagc ttgtgacgga
    43021 gttcttctag ctcttgctcc ttatcgtcaa attcaatctc acggctgagc tgagttgata
    43081 aacagttgac tgccaacaaa agagcgattg tttcatcatc tgcgctaggc atttgttctt
    43141 taattgcttg gtatttttct gtcgcaacct tagcgatttc ctccataaaa aggttatcat
    43201 gttcgcttgt caaggttaac gattttttcc cgaatgtaaa tttgaatcga tttagatttg
    43261 ccataaaatt cacctcacga tattatacca aaattcgcta attttgtcag tttttacaaa
    43321 ttttactgct tttgtggtac aatagaaact atggcaagta tcacactcac accaagcgaa
    43381 aaggatattc aggcttttct tgaacactat caaaccagtc tggctcctag caagaatccc
    43441 tatatccgct actttttgaa actacctcaa gcaacggttt ctatctatac ttctggaaaa
    43501 atcttgcttc agggtgaagg ggctgaaaaa tacgccagtt tctttggcta tcaagctgta
    43561 gagcaaacca gcggacaaaa tcttccttta attgggacag atgaggtggg aaatggttcc
    43621 tactttggtg ggcttgcagt tgtggctgcc tttgtcacac ctgaccagca cgacttttta
    43681 cgaaaactcg gtgtggggga ttctaagact ctgaccgacc aaaagatccg tcagattgct
    43741 cctattctca aggaaaaaat ccagcaccag gcactccttc tctcacccag caagtacaac
    43801 gaggtcatcg gagaccgcta caatgctgtt tcggttaagg ttgccctcca taatcaggct
    43861 atctatctcc tccttcaaaa aggtgttcag cctgagaaaa ttgtgattga tgcctttacc
    43921 agtgctaaaa attatgacaa gtacttggca caagaggcca atcgtttcag caatcctatc
    43981 agcttagaaa aaaaggctga gggcaaatac ttggctgtcg cagtttcttc tgtcattgcg
    44041 cgtgatctct ttctggaaaa tcttgaaaat ctgggacgag aactgggtta tcagctccca
    44101 agtggagctg gaacagcttc tgacaaggtg gctagccaga ttttgcaagc ctatggtatg
    44161 cagggactca gcttctgcgc caaattgcac tttaaaaaca ctgaaaaagc gaaaaaacgc
    44221 ttagaaaggt aagttatgaa ttcatttaaa aatttcttaa aagagtgggg attgttcctc
    44281 ctaattctgt cattactagc tttaagtcgt atcttttttt ggagcaatgt tcgcgtagaa
    44341 ggacattcca tggatccgac cctagcggat ggcgaaattc tcttcgttgt aaaacacctt
    44401 cctattgacc gttttgatat cgtggtggcc catgaggaag atggcaataa ggacatcgtc
    44461 aagcgcgtga ttggaatgcc tggcgacacc attcgttacg aaaatgataa actctacatc
    44521 aatgacaaag aaacggacga gccttatcta gcagactata tcaaacgctt caaggatgac
    44581 aaactccaaa gcacctactc aggcaagggc tttgaaggaa ataaaggaac tttctttaga
    44641 agtatcgctc aaaaagccca agccttcaca gttgatgtca actacaacac caactttagc
    44701 tttactgttc cagaaggaga ataccttctc ctcggagatg accgcttggt ttcgagcgac
    44761 agccgccacg taggtacctt caaagcaaaa gatatcacag gggaagctaa attccgcttc
    44821 tggccaatca cccgtatcgg aacattttaa gagacctaag aggccgagaa tcaccaatct
    44881 cagcctcttc ttctatcgtg agaagtgatt gtttattgac aaatactcaa cgaaaatcaa
    44941 agaacaaact aggaagctag ccgtaggctg cttaaaacag tgttttgagg ttgtggatag
    45001 aactgacgaa gtcagctcaa aatactgttt tgaggttgca gatggaagct gacgtggttt
    45061 gaagatattt tcgaagagta taaaataaaa acaatcccaa ctctcaccta ttcatgcaaa
    45121 ggaattttat ggaagtttat ttttcaggaa ccattgaacg gattattttt gaaaatccca
    45181 gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag gattttgatg
    45241 attttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa gactatactt
    45301 tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc agtcgttatg
    45361 atcgcgcaaa accaactagt aagggcttgg tcaagtactt ttcaagtagc catttcaagg
    45421 ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat accattgacg
    45481 aaattttgca acacccagaa aagttagaag gcatcgcagg actctctgcc aaaaatcgcg
    45541 aggctttcgt ctccactctc cgtctcaact acggaacgga gatggttttg tccaaactag
    45601 ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac aaggaagaaa
    45661 cccttgatgt ggttgaaaat tatccctacc agctggttga ggatatcaag ggtttgggct
    45721 ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct cctgaacgct
    45781 tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacaggg gacacctatg
    45841 ttgaagcacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc cgtcccgtgg
    45901 aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa gacaaggttc
    45961 agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa ggcatccgca
    46021 gtcacttggt tcgtatcctt gaaaaaggaa aacagaagag tcatgattta gaaaccattc
    46081 aaaaacatat cactactatc gaggaagagc tgggaattga gtatgataac attcaaaaac
    46141 aggctatctg tgatgctatc cagaataagg tctttatcct gacaggtggg cctggtactg
    46201 gtaagacaac tgttatcaat ggaatcatcg ctgtttatgc cctgttagag ggacttgact
    46261 tcaggaagaa aagcaatctg cctattcttc ttgctgctcc aactggtcga gctgctcgtc
    46321 gcatgaatga attgacaggt ttgcctagcg cgaccataca tcgccacttg ggaatgacag
    46381 gtgacgatga taccagtcat ctggaagatt acctagatgc tgactttatc atcgtggatg
    46441 aattctctat ggtagatact tggctggcca atcaactctt ctccaatatc tcttctaaca
    46501 gtaagatcct catcgtaggt gacagtgatc agctaccttc tgtcagtcct ggacaggttc
    46561 tagcggatct gcttcatatt cctttgattc ctcagactca cttggaaaaa atttatcgac
    46621 aaagcaaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc atcttgccag
    46681 ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt ggccatattc
    46741 ctgccaccat tgaaaaaatc ttaggtgccg ccctcagaaa tggtattcct gcccgtgata
    46801 tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc aatcagctca
    46861 tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct cctcagtgcc
    46921 actatcgtaa gggagacaag gtcattcatt tggttaacga tgctgaaatc aatgtcttta
    46981 atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa tcgaaacaag
    47041 acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac gaatggtaca
    47101 agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag ttcccagttg
    47161 tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc atctacacag
    47221 ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc ttcgactatg
    47281 ctacccaaca catcggaact gcccgaaaaa cctatctgat tgaacgcttc agtgatctac
    47341 tggagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca agtgcctctg
    47401 aacaatccta catcctaacc gaagaaaact gggacagcat tccagccatg attgggatta
    47461 cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaacc accaattcag
    47521 gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtaatc aattcaacag
    47581 cttttttgat agtgatatca tgtttcaaca tgtcagctga aagcaagttt tgaacttgtg
    47641 caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt tcttcttctg
    47701 aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc ttagtacgtg
    47761 actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca gtaatttgga
    47821 agtacatgtc agggttgatc ccttgacgtt gcaagttccc aaggaattca tttactgaac
    47881 ggtgaacttc ttcgtggatc atttcttctg gaagttctac gatttcagca ttttctacag
    47941 ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta gcagcagcca
    48001 attctttgcg gtatttttct ttcaagtcag caagtgtttc aacttcttca tcaatgtctt
    48061 ttgcaagttc atcgtcaaga gctggaactt ctttagcttt tacttcgtgg atagttgtca
    48121 cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg aatgttacga
    48181 taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa ccagggatga
    48241 attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca aattcaacac
    48301 cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gtcttcagca gcagcttcct
    48361 tgataaccaa ttcagccagg ttgttgcgtt cacgttcgat acgctcttcg acatcagcgt
    48421 cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca cccaatttta
    48481 cttcaggttt tgtaacaact tcagcagcga taacccagtc ttgacctttt tccattgaag
    48541 ttacgtcaat ttttggttgg gcaaccactt caagaccagc ctcttttaca gctgcttcat
    48601 aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca ccaaattttt
    48661 tatcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta agagatttct
    48721 tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag atagtgaaag
    48781 tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg tcatttctcc
    48841 ttaaaatttt ttaaatacag tctattataa cattttctat aaataaaatc tagactaatg
    48901 cagacaatta tagacaacaa gcctttttcc taactaaaac actcataaag tatagacaat
    48961 tttagactac tttggatatc ttttggttag tcattttcca aaactaattt atctaatttt
    49021 taagttatat ttgacttcat cttctcagta acatgagtat aaacacttaa aatctttgtt
    49081 tcattagtat aatctacacg atccattatc gctgttatag gtacttccaa ttcagcaagc
    49141 aaacttatat gcgaatatct aaaaacatga gcagtcaata caaaatcatc tttcttaatt
    49201 tcacttttcc taaacctgtt tttcaaaaag taattgaaac aagttatcat taagggagaa
    49261 ccattatttg taaatatata atcaccttct tctaaaagaa gatattcttt taaaatttct
    49321 aacactctat cactaatacc aattactcta tatgaagcta aacttttcgg actatccaaa
    49381 aacacttcgt tattggacga ttacattata gtaaaacgaa ataagaacag gacaaatcga
    49441 tcaggacagt caaatcaatt tctaacaatg ttttagaagt agaggtgtac tattatagtt
    49501 tcaatctact atacaagacg aaattgaaga aatctttcca ttataaaacg catattatca
    49561 aggtttttaa acacctaata atatgcgttt tttcctattt taaagacttt tatccaccta
    49621 ttatacaaag ttaagtatcc atcacagttt acaattagcc ttcattacct aacccattca
    49681 ccatccttca agagagtaac ctttaagaac cgaataaata aatggcaaat ttcgttcatt
    49741 taatttaact gcactgttaa tttgattagc tccatctcct aaagtatgca ctcctactac
    49801 acggtgacta gcatcataaa ctgtagatcc actagacccc tctaaagtat caacttggta
    49861 atccaagaac atgccatcat cacttaaaac ttgtttttta tctgtataca tttgatgaac
    49921 tttaaaatta tatgatggat agcctgtgat agtcacagtt attcctgtca aatttttttg
    49981 actagtagga agacccaaag tccctaattt tgcaccaatg ggctcttcta gaattaataa
    50041 agccaagtca tattcccttg catccttaga atttaaattt ctaaattcct tcaaataacg
    50101 aacttccttt actttgatct ttccaaatgg ttcttgactt ggactaacag tcggaagaac
    50161 ataaatatca tccgcttctt tcccatagtc atgtctgtag tagttgtgag ccgctgtaat
    50221 taacacatta tctttaataa aattcgcact tcccctccct tctacattca ttccatcggc
    50281 tactccataa tacttagata aaaccatagc tgaagtcgaa tacggatact gtaaagtatt
    50341 atcaatttta atcaaatcat cattaccgat aatacttctg attgcttttg gtagtatgaa
    50401 ccatatgttg gtgaaatctc agataatgaa gaatcattag atcctggacc tttttctagt
    50461 gtctcactta cctcatattc ttcacccgta ctagaaataa cactcaaagc agatactgtc
    50521 gataactggc tagccaataa agtactcgca ataattgaaa tacccaattt tttataaaca
    50581 gttttcttca ttattgtatc ctcctaatgt aattatagcg tactattcta aatttcttaa
    50641 tctactatag aatcaagaaa tctaccacct tctttaaata ccctccatta tcacataaac
    50701 aggtaaactt ttcaattaat gactgcgctt ttcaatcacg ctagaggtac ttacttgctt
    50761 ctttgatact aagttcagcc attctttcct tgtttttctc aataaagcat gttacccaag
    50821 tgggattcgt tttggagtag tctcgcagag tccagccaat ggctttattg ataaaaaatt
    50881 ctgtttggtt caagttatga agtagaatct tttccattaa ttgagtattg gtcttctctt
    50941 ttcttaacaa ctggtggtca atagcgacac gtctcagcca gatattatct gataggctcc
    51001 attttatact caatgaaaat caaagagcaa actaggaagc tatctgcaac ctcaaaacac
    51061 tgttttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag agtattaaga
    51121 ttatttcttc tagttcaggg tgttcataca ccaaactccc tactactcga tctaggatat
    51181 ctaccgtgtc ccaccaggat tttgtcacga ctaactgctc tagcttaggc aaatcggttt
    51241 cctttagata agactgcatt gctttcaaat agttagcagc cacatattgg tattttctag
    51301 gctccttttc ccagcaagtg tctgcaaaat cccaatcgat aatctttgtt tttttcgctt
    51361 ctggtaaata tttttataga gtttatttct ttcaggcacc gcaataccta gaaaagaaaa
    51421 ttgatggcgc atataggctt ccatgggcct tgctttttta gagtcttttg ctgcttctag
    51481 ctcctcaagt aaatctgcta aactcatcta aaactcctct tgccccacca aatggtgctg
    51541 aaaggcatag acagccgcct gggtacgatc gctgacttca agtttggcaa gaatattgga
    51601 cacgtgggtc ttgaccgtct tgagagagat aaagaggtca tctgcgatgc gctgattttc
    51661 gtagcccttg gcgatgagtt ggagaacatc tcgctcacgc gcagtcaatt cttcatgaag
    51721 ttccatatga ttgcggtggt attcaacctt cttgctaacc tcttgctcaa tggccagctc
    51781 gccagcagct accttactga cggcatgaag caattcatct gcactagaag tcttgagcat
    51841 atagcctttg gcaccagcat ctaagactgg catgattttt tcattgtcca aataagaggt
    51901 cacaatcaaa atcttggctt caggccattc tttaaggatt gctaaggtcg cgtcaatccc
    51961 attcatctca ggcatgacaa tatccatgac aatgacatct ggacgcagtt ccaaggccaa
    52021 gtcaatccct tgagacccgt tggacgcctc acccacaact tctacatcgt cttggaggtc
    52081 aaagtagctt ttcaagccca aacggaccat ttcatggtca tctactagta aaattttcat
    52141 ctttactcct ttatcattcc ttatctaaca ggggaatacg gatatcaact gccagccctt
    52201 gcttgggagc tgttaataac tgaaccgtcc ctgccatatc ttcaacccgc tccttgatat
    52261 ttcgcagtcc ataactcaag tcgtctaagc ttcctaaccg gaaaccaatc ccattgtcca
    52321 ccaccttcag ttgcaattca acatctgtct gatagaggta gacatctagg caagatgcct
    52381 gggcatggcg gagggtattg ctaatcaact cttgcaggat acggaagata tgctcctcga
    52441 ttttcttagg caatttcgtc atattctgct tgagactaac cctaagatca ctcttgtcct
    52501 caagctcttt taagagaatc tgaatccctt ctatcaagct cttctgctcc agttcaactg
    52561 gtcgcaaatg caagagcaaa acccgcaaat ccttctgggc agtttctaaa atagctgtga
    52621 cactctgcaa ctgggtctgc atcttttctc tatccaattt caaagcctgc tgactgatac
    52681 ccgataaaat aatgtgggcc gcaaacaact cctgactgac tgtatcgtgc aaatcccgag
    52741 caattcgctt ccgttccttc tcgataattt cctcttcctg atcaaggcta tgattttcag
    52801 ctttttgaag agcctctgtc aaaaggttaa gtttacctga taaggacttg aaactggcat
    52861 ccaaatctgg atctacaacc tgaactactt cttgccctgc caataaacgc ttgagattag
    52921 cctgcatttt tcttagagaa agctcttcga tccctcgcca aaacagggct aagagacagg
    52981 tcatggacat gctgaaaacc aacaataaaa agacaaattt ttctgttttt tcgacatcgt
    53041 acaaaaagat agaccagtca aaatcaagta tttccagcaa gctgtgggag aaaaaaaaga
    53101 caaataggaa ggaggtgaga gcaataatga cataggcttg ttttttcatc ctctaaccac
    53161 ctccacatca ccaatcatag tggtcaagaa aatcttgaca ctcttgttac tcttgagata
    53221 gttttttgtt tcttgatgat agtgttcatt gcggagggct cgcttgggct ggttgaaaaa
    53281 agtcaaatcc ccatagagac agttaacgct gagactgact tccacatcta caggtacgat
    53341 gattttggtc gttcctacca tctttctgag gataatgaca ttgtcatgat tggttaagat
    53401 gaccctctcc agatgaatag tgtccttgcc catgaagcga aagagattga tatcatcgaa
    53461 ttggcaagtc tggtagcttg aaaaatgatg aagatttcca aaccaacgat ttttctcctt
    53521 cttaaccgtc acaacctctt caaaaaccaa attggtctgc tctttttcct ggttcatcat
    53581 cgggtaaaga agaaagaggc tatagataac cgcaacaaaa atagctagaa tcacaaaagg
    53641 attgagcata acgatgaaaa agaagagaat ggttaccgct actaaaagaa gattatttcc
    53701 ctctttacca gtgtagtagc gaatcaaaag caaaaagagg aatagtatca gcagaaaacg
    53761 cgaaaaatgc tctgatacca tcaaaatcag agctcctgtc agaagacagg cttcgataaa
    53821 taaaaagatt ttaaattttc tcataggttc atcctctccc ttctatttta tcacaattca
    53881 aaaaagtcac ctcagtctga ggatggaaaa aaaggcgctg gttacgcctt tttcatctga
    53941 tcatttgctt cttttaattt tccataaaga agatagtcta ctttttgtag atctgctatg
    54001 gtggcacagt taagggaaca cataatcaag cgtagatctg ctttccagcc ttggacaatg
    54061 ccaagcactt cttcaactgt gtaggtttca accaattcca gaacggttcg tgacaatccc
    54121 acagccttag caccaaaaac caagcactta atcatatcca gcggattccg aacccctcca
    54181 ctaaccaaga gttcgacctt atctttccat tcttgggcat tgagaagggc ctgcatggta
    54241 gactgacccc attgattgag gtaatcacgc tggccactac gacggttttc gatataggca
    54301 aagctggtgc caccacgacc cgataggtcc actgtacgaa caccgaattc ataggctctt
    54361 tcgattgtct tggcatccat tccaaagccc acttccttga ggacaatagg aacgggaatt
    54421 tgcttgctat aatctgctag atgcgattgc cagcttctaa acttcctttc tccctcgggc
    54481 atgagtaatt cctgcatgac attgacatgc acttgcaata gaacaggatt catctcttct
    54541 acagtatgaa gtcctaactc gacaggcttg tccaatccaa tattggttcc aaggaggaga
    54601 ttgggatgac tagacttgac agaaaaagaa tcatccgttg gatttttgag ggctgcgcta
    54661 taagaacccg ttacaaataa aataccacag gattccgcca cctgagccag cttttgattg
    54721 atttctcttc ccttattact tccaccagtc atggcattga tataaaaagg aaagtcccac
    54781 tttcgaccag caaactctgt cgaaagatcg atttcatcca gattgtaaag aggcaaggaa
    54841 gaatgaatca gctccacctc atcaaagcta ttataggaac ttttctgctc aagggcatag
    54901 aggatatgct cgtccttacg atttgtcgtc atgtcctatc ctttcttgat ataagagctc
    54961 aatccccaga tcggcccaac gattttttaa ggttttggtt gattgcgcat caaaactcag
    55021 ggcgatgcca cagtcaccac caccagcacc actactcttg gcaacggcct gcaaatcttg
    55081 actggcttct ttcaactgtc taagcaaagg cgtgtaaata tctgtactca agccttctaa
    55141 aagcttgctg gctacttcta cttgctcgat aactttttcg gctttcccct gctccaaggc
    55201 ttcgaccaaa gaaaccaccg tttcttttga ggaacttaaa aaattttgat tgatattttg
    55261 cttgatttgc tggaccatgt gactcgatac agccacttcc ttggtccatc ccactaagaa
    55321 atcacattct aaagttggtt tcacttgtga gataaaaaat ccccaatcac gctccagaac
    55381 tgtcgccaag ttttcttctt ctaaccaagc agccgccttc tggcgatcaa atgactggta
    55441 gagaaccaaa tcctctgcca caatacaggc aaggtcgccc atggaaccat tgtctcctcg
    55501 cttgagcaag acagcgctag tcagcttgaa caagagattc tgatcaaccg aaagattata
    55561 gagagccagt aaagccttga caaccaagac aacgacgctg ccactagaac ctagaccaaa
    55621 ctttttccct tctcgttcca ttttgccaca gatttttagg gaaaaaggtc ttaaattctg
    55681 accgcgaaca gcgaggaagt ctcccatcaa agcaatcgtt tcttgaatca agctgtagtc
    55741 aggattaggc cttaagtcca ctgcgaaatc aaacatatct gaatagatac ggtagctgtc
    55801 agaaaaagca atctcagctc tcatatagat gggaatatcc tttatcaaag ctaactgccc
    55861 tggctctaaa atagcatatt cacctgccca atagagtttt ccgcaagttt taacagcaat
    55921 catcttgact caaatccttt gtttttgaca caatcaagcg ataacgatga ccgaaaattt
    55981 ctgataaatg ctccaagtct ttctcctgac agaagacctt aacattggga ccagcatcca
    56041 tggtaaagta gcaggcctct cctttctcac gaagctggcg aacaaagtcc atagcctcat
    56101 aagaggcatc cgtcagataa gaaaaggctg gactagcagt ctttgtcgta gcatgcatag
    56161 ccagggcatt tttctccgtt aattctccaa tcttggcaaa atcattttcc ttgagataaa
    56221 tcagcatatc ctgatagtcc ttctcagact gacgaaccca gtcgtcgaaa gtcgtcgagg
    56281 tttccacaca aagtttcatc ccgtcacggc tagagattgg ttttttcttg tcctctagca
    56341 ccaacataat catagctagt ttcaagtctg tctctacagg gtaaatttct ccactatcct
    56401 tatcccaggc tcctagtggt ccataaaaac tccgagaaga agagcctgag gcaaacttgg
    56461 cttcctgcgc taactgactt ctatccaatc caagcttgaa ataagcatta caagccttga
    56521 ccagggcgga caaaccacta gaacttgagg acaggcccgc tgccgtaggc atattgtttt
    56581 gagtatcgat acggacaaag ccctcaccag ctggacgata acggtcaata atcttactca
    56641 tcttggcatg ctcgacctca ttttgtagct gaccattgat gtaaaattcg tcagctgtta
    56701 cattggctgg taaaggcgac aaggtcgtct ctgtatacat attttccaaa gttagagaaa
    56761 tactgctagt agcaggcacc atctcttttt cttttttctt tccccaatat ttaataatag
    56821 caatatttgc gtaggaacgt actgttacag gctctctatc catgtctgaa cagctccttt
    56881 ctcttctaat ctttctgcta gttcttgtgc gtgtgtcaaa ttggttacca aggctatgat
    56941 acaacctcct agcccaccac cgctcatctt ggcacccaga gcaccatggc taagagtcgt
    57001 ttcaaccaaa aagtctgcct cagggctact gactccaatt tcttttaaat gtaaatgcgc
    57061 ttgactgagg atttgtccca gtccttcagc atctttttgt gaaattgcaa cttctgcttg
    57121 ctgggttaat tctcccaagg catgcaaaaa cggtagggca tccttgccct tattttgaac
    57181 cacttggatg gcttcacgag tatgaccata aacacccgta tcggcaatca ccaaataggc
    57241 ggataaatcc atctcaagtt ctgtaaatcc tacgttcttg ataaagcgaa taggttggtc
    57301 actgagacag gtcttagcat ccaaaccact aggattcata tgggcaatca tttcagctcg
    57361 attgaccaag atttctagta catcatgagg cagatcagcc tgatagtagt caaataccgc
    57421 acgaatggcc gctatgctga tagccgctga cgaacccatc ccccgtttct cagggatagc
    57481 cgagtcaatc tcacaacgaa tgcaggcttc tgtgatatcc aaatactcca gtgaggcata
    57541 aaccgccatg gacaaggtat cctcctcata aaggcgccaa ggactctctg caggaactac
    57601 cttacaggtc acctccacct ccaaaagagg cagggaaatg gcaggataac cgtaaacgac
    57661 cgcatgttcc cctattaaaa ttatcttact atgtgcctga ccgacaccaa ctttttttgt
    57721 cattttttcc ttttactaga cgaaaaaacg tcttattttt catacaagta ttaattcttt
    57781 cctatctatt ttattatatt ttcacaaaaa aagcgattgt ttccattcac aatcgcttct
    57841 ttcattattg aacccattcg ccattatagt tgacagaata gccatctacg gtcgtattca
    57901 ctgccaaggc acctgagcta taagcatagt accatttgcc attgacctgg aaccaacctg
    57961 tcttcatgtc tccattacct gcatttaggt agtaccaagt taaaccatct tgataccagc
    58021 cagttaccat agctcctgat gaacggaggt agtaccactt gttaccaagg tattgccatc
    58081 ctgttttcat atcaccattt ttattatcta agtaatacca tttgcctcgt acatatttcc
    58141 aacctgttgc catagaacca gaggagccaa aataatacca ttttccgttt actttttgcc
    58201 aatctgtaag catcttacct gaagcatcta ggtagtacca tggagcaggt ttgcttctat
    58261 caatgtcaat agtaacatga aaatcttgag tccaaccctt agcaacttca ccaattggta
    58321 gtggattgta agaatcatcg ccaaaatttc ctagcttatt taaataatac caattgccgt
    58381 cttcataaat ccagcctgtc tttacagcat aattattatc aaaataataa gttctctttt
    58441 cagcagatag aggatattgt tcaccgtata cttttcctgt attttcagac gtttttgcct
    58501 ctagtacttg tttatctgtt tgttctagca aagcaccatc ttgtccaaaa tagtaccact
    58561 tctcctgaag gccaatctca ttaactggtt ggttatcgaa taaattatca cgataacctg
    58621 ttccaggaat ttctaagtat tgccaaccga caaccatctc tccaatatca ccaaaatagt
    58681 aagttttacc gtctatttta tgccaataga ttgctttatg atcatctttt acataatatt
    58741 ttctattatc cttatcaaca aatcggccac ctgtggtatt cgcaaatacc gtatttgtag
    58801 ctagcaaacc aaagaaaaag actgctagta gaacttgcat catttttttc aaaagtttca
    58861 tctatatatc ctcctaaaac gattgtagca aagacccgac tgcatgtcaa tatataaaaa
    58921 ctcctcaaaa aagcagttac acaactgcaa ctgcttatct attcttgcat ggtatatcca
    58981 acaccacgca cggttttaat gtagcttttt tggcctttaa catcaagctt gctccgtaga
    59041 tagcggatat agacatctac gatatttgtc tcggtcgcac tttcatactt ccaaacactt
    59101 tccaacaatt gctcacgagt caatactttc ttgcttccca taagtgtcgc caaaaggtca
    59161 tactcacggc gggtcagagc aatcatctct tcaccacgat aaaccgtgtg atgttcaaca
    59221 tctatcctaa gattgcggta ggtccttgga actttcatca gactgcagtg ttgatcaatg
    59281 aagtcccgac ctcggaagat cgccgaaata cgcgctacca gattttcgat aaggactggc
    59341 ttataaatgt atgaaactgc aaaacgctga acaacttcca gctcttcttg caagtcttcc
    59401 caatgatcta aaatcatgat gactgaggca ggtttagttc gactcaattt ttctgcaaaa
    59461 tcctgagcca tcatatctcc cagattaacg ttcaataaca tcaaatcata gtctgtctga
    59521 agagccatgg agagggcttt ttgcccctcc tctaccagat caacccgata ctgctctttc
    59581 tggagttcca aacttaaaaa atgagctaga tttcgttctt tctcaagtaa taaaatccgt
    59641 ttccccatgg ctgacctact tatttttcgt cataccaaga gtagtggaag gttccttctt
    59701 tgtctttacg ttggtaagtg tgagcaccaa agtagtcacg ttgtgcttgg atcaagttag
    59761 ctggaaggtc agctgaacgg tagctatcaa agtaagtaat agctgctgag aaagttggca
    59821 ctggcacacc agcttgaacc gcaagagcta cgatatcacg cactgcttgt tgatacttag
    59881 cagtaacatc caagaagtac tcgtccaaaa gaaggttggc aagatctgca tcgcggttgt
    59941 aagcatctgt aatcttttgc aaaaaacgag aacggatgat acagccatca cgccagatag
    60001 atgcgatatc tgcaaatggc aagttccagt tgttttcttt agaggctaca cgcaattgag
    60061 caaatccttg tgcgtatgaa atgatttttg agaagtaaag ggcttgacgg atcttttcaa
    60121 tcaattcagc cttgtctcct tcaaagttga aggcagctgg ttttggaagc accttgctag
    60181 catatacacg ttcttctttg taagttgaaa tgtagcgtgc aaacactgac tcagtaatca
    60241 gtgacaatgg tacaccaagg tcaagagatg attggctagt ccatttacca gttcccttgt
    60301 tacctgcagc atcaaggatg tagtctacga ttggtccatc ttggccttca tcgtctttac
    60361 ggctcaagat atcagctgtg atttcgatca agtagctatc caattcaccc ttgttccact
    60421 cagtaaagat ttcagccata tcctctgcag aaagacctag caagtgttgc atcaagtcat
    60481 aactttctgc gatcaattgc atatcaccgt actcgatacc gttgtgaacc attttcacat
    60541 agtgaccagc tccatcagga ccgatataag tcacacatgg tttgccatct tctggtgctt
    60601 tagctgagat ttcttcaaga acatccgcaa ccaattcgta ggcctctttt tgtccaccag
    60661 gcatgataga aggaccttca agagcacctt tttcaccacc agaaactcca gtaccgataa
    60721 agttgatacc tgagtttgcc aattcttcat tacgacggat ggtatctttg tagaaagtat
    60781 ttccaccgtc aatcaagata tcacctttgt caaggtgtgg aagaagggct tggatagtag
    60841 catctgtacc aggtccagct tgaaccatca gcatgatacg acgaggtttt tcgattgagt
    60901 ttacaaaact ttcaacgtca tagcttggta caaaattctt ttcaggatgg caagcaatca
    60961 catcttccgt tttttcttta ctacggttgt agatagcaac tgtgtaacca cgtgattcaa
    61021 tattaagggc aaggttacga cccattacgg ccatacctac gacaccaaag ttagcttttg
    61081 tcatttgaca ctcctcttgt ttcatttgtt ttattttatc atttttactt ttgaaaagaa
    61141 agaattttgt aacgactgct tagtagtcca agtcatccgc atgaccagcg ccatttccga
    61201 caaagtagcc tgtcttacag ttaagggtaa agagataggt tccatctggc ttatagaggt
    61261 tgtaataacc attgccgtta acgatattga cacgttcaag gatatattgg tctccagtga
    61321 tatagccacg tgaacgtgaa gtcgctagaa tttgttccaa gacaccatcc gcaaaatccc
    61381 aggcagagtt attactatca tcaatagcag actgattata ggcaacacga ctggcacttc
    61441 tttgaacagc aacccctgca ctcgagagac ccatattgac ttcactgcga ctacttctag
    61501 tctcatttga actacttggt tttggctcac tagtcgtatt tgaacttgct tgacttgaac
    61561 ttgagctgct agtttgactt aaacttgagc tagaagtact tgtttgctgg ctcttaccaa
    61621 gactgatagc cttatctagc actttatcaa gctccgtatt tccagtttta atatctgtaa
    61681 atttagcatc cgatttggct ttggcattgg tatccaacac accatccaca atagctgatt
    61741 tttcaaattg tgcattgaca tcttgaatag ccttgatttg cgttgctaga ctatcatatt
    61801 tagatttggc aagcgtatgt tcacgactac cttccagctt atcaagtaaa gtcttgagtt
    61861 gattcagttt atcaaactgg ctatttttca aagccgtttt attgctatct gtgtaaaagg
    61921 cgtcataaag tgtattaaaa tcatccacat ccgattgatt ggctgtagtt gattgagaag
    61981 tttcaatttc cttggtcgaa cgagccactt gacggtagac ataataagca ctgacacaaa
    62041 taattactga tacaagcgcc aaaacggtca aaataaagcc ttttttactt ttcttttctg
    62101 agtcctcttg atccagacga gaaaggtttt gctcctcttc cacttcttca gtcatttctg
    62161 ttggattttc atcatctagc aatagaggat ccaagccttg ctcctcatcg tccagaggca
    62221 agggtagtaa gatgtcttca gaagatggaa cttcctccga agaagcttct gacttctcta
    62281 cagcctcacg catctcttga attaaatcat ccagactctg agtttcaacg aactcctctt
    62341 ttttgtattg acgagtcgca aacttatccg cctcaatttc atctctgtgt tgcttaacat
    62401 acttgtccaa aatggaatcc tcaggcaaga ctcctgcttc cacttcttca tttttacgaa
    62461 tagcttgacc aactgttagc tcttttgctt catcaaaatc aaattgcggt tcttgacctt
    62521 cttttttttg acgatttcgt ctttttttac tcatgagtat cctttctatt ttacctcttg
    62581 ccgtttgaca cgctgaccaa aatattgata tagatccact ttcaaggttc cgttgtataa
    62641 cttacgcttc ttatctgctt ggctaccata cttgctttca aaagcttcat cactagtcag
    62701 gataaatttg ctccaagttt tcagcggtgc aaatacttgc cccatctcag catagagctt
    62761 ggtcacccct gcatcatctg acaaacgttc accataaggc ggattggaaa tgattactcc
    62821 attgatttta tcggaacgta aatcctgcac gcgcatctgc ttaaaagtaa tgtctcctgc
    62881 aacaccagct acctgagcat tggccttagc aatttccacc atgcgagcat caatatcaca
    62941 gcccattata tccagctcaa gctcacggtc tactttttta gccgcttctg tgcgcacttc
    63001 ttgaatcaag cgatcgctga tccagttcca ttcctcaaat gcaaaagagc gacgaagacc
    63061 tggcgccatc tttctagcaa tcataactgc ctcaatacag aaagtccccg aaccacaggt
    63121 cggatcaatc aaaggcttgt ctggatacca gttagaaagt tgtaaaatgg ctgctgccat
    63181 attttccttg ataggagcgc cacctttttc ggtacgataa ccacgtttaa agaggctaga
    63241 cccggtcgta tcaatcatga cagttgccac atctttgaga atagagacct caatcttaaa
    63301 ctctgggcca ttctccatca gaggaacccc ttctgggcga gcatagtgtt tctgcaattt
    63361 cttgacaaca gctttcttag aaatagcctg aacactgggc tcattgtgaa gtttggactt
    63421 aacacattta gcttttgaaa tcgggaaccg agctccaagt ggtaaataat tttcccaatc
    63481 caaagcgaaa actccctgaa atagctcttc aaaagtctta gctgggaacg ttcctacgat
    63541 aattttgata cgatctgctg cccgaagcca aaggttggtt tcgataatag ctctcacgtc
    63601 tccttgaaaa cgaacacgtc cattttcaac ctgacaatcg tagcccaact ctcgcacttc
    63661 acgaccaacg acagcctcaa gccctgctgc cacagttgca attaaattaa attctttttt
    63721 catgttacag tcttgcaaga cctttctata tgtccacttt aaaaaatgag gttgagaaaa
    63781 tttctcagcc ccaacctata tgcaattttc tataagccat gttttgttcc agaagtgact
    63841 aggaccactt ccttcgataa tcatctgtct accactaaca gctctagctg atagcagtca
    63901 gaatactacg ttcgtttccg cgtactccat gccccgacca aaatttgggt tgctagcttg
    63961 aggggtttac cgcgttccac tctctctgtt tccagaaaga ctccgtcact gtggcacttt
    64021 caagcctact ctggcctatc caaggactta gccatttcaa ctgccgtaac gatttctcgt
    64081 ccctaggctt atggtttcgc ctagcacaaa cactacaggc atcacagcct gtgctagcat
    64141 ggactttcct catgagaagc aattctcctc acgcgattat ccaaaaattg cacatctcaa
    64201 ataactactt aaaaatctga gttatctaaa atttgtttac caaaaacttc tttttccaat
    64261 ctattcaggc gtttcaaaat atcaaaattc gtcatagaac ttgtaattgc cgcttcaagg
    64321 ggttctgctt gaactggtga aggtttcggt ttacgagtta attcttcctt caaatccgca
    64381 atttcctgac gaagtgactt gaccaaggca gcataggttt catagtcctt gatgacatcg
    64441 tctaaaaact cgtcaacttc tactttatta tagccacgga cttcacgtcc aaactcttgt
    64501 tcaaaaatat ctttcgctga aaaaataata cttgccatgt ctctctccat tctcgcttgc
    64561 tagtattatt atataaaaaa aggcaaacaa aaatcaaggt caaagcttca attttcggaa
    64621 aaattttcag caagttcatt taattgatca aatgttaatc tctttataaa atagtcctct
    64681 tgatttttca tcttttggta aaaataagct agttttgttt cattttcttc atcataaaaa
    64741 agataggaac tagtcgtgtt ttccagcaaa aattgctgat aatctcttaa ctgccccttg
    64801 tgttcataac gaggataagc atatttgaca aagtctacct gcttaaagcg actcagtttc
    64861 atctgattgc cttcattcca attttcccca tgggtttcaa aagcaaaaat ggtggccaac
    64921 tggaaaccgt attcggtttt catctcctga gcaacctcta aaacccaata ttcaaaaccc
    64981 aaacttcctg taaacacaag ccaagacacc ccatctgctg ccatagcctc taaatcttta
    65041 cgtatcgctt ttttaatcaa tttaagacga ggatccttat cagaaaataa acccaaatca
    65101 aaagcagaat agcctaaaac caaagctgta gccattttta accctttctg tgcaaattat
    65161 ggtataataa agtgatgtta ttgtaccaat aaggagaatt atggtcaact atccacataa
    65221 agtttcatca caaaaaagac aaacatctct ttctcaaccc aaaaatttcg caaatcgagg
    65281 aatgtctttt gaaaagatga tcaatgctac caacgactac tatttgtctc agggcttggc
    65341 tgttatacat aagaaaccaa ctcctattca aatcgtacaa gtggactatc cacaacgaag
    65401 tcgtgccaag attgttgaag cctattttcg acaagcttca acgacggact attctggcgt
    65461 ttataatgga tattacatcg actttgaagt caaggaaaca aaacaaaaac gtgcgattcc
    65521 gatgaaaaat tttcatccac atcagattca gcatatggaa caagtccttg cccaacaagg
    65581 aatctgcttt gtccttcttc acttttcttc tcagcaagaa acctacttat tgccggcatt
    65641 cgatttgatt cgcttctatc atcaagataa gggacaaaaa tcaatgccac ttgaatatat
    65701 tcgagaatat ggatatgaaa tcaaggctgg tgccttccct caaattcctt atctcaatgt
    65761 tatcaaagaa catttattag gtggtaaaac aagatgaaca aaccaacgat tctgcgccta
    65821 atcaagtatc tgagcattag cttcttaagc ttggttatcg cagccattgt cttaggcgga
    65881 ggagtttttt tctactacgt tagcaaggct cctagcctat ccgagagtaa actagttgca
    65941 acaacttcta gtaaaatcta cgacaataaa aatcaactca ttgctgactt gggttctgaa
    66001 cgccgcgtca atgcccaagc taatgatatt cccacagatt tggttaaggc aatcgtttct
    66061 atcgaagacc atcgcttctt cgaccacagg gggattgata ccatccgtat cctaggagct
    66121 ttcttgcgca atctgcaaag caattccctc caaggtggat caactctcac ccaacagttg
    66181 attaagttga cttacttttc aacttcgact tccgaccaga ctatttctcg taaggctcag
    66241 gaagcttggt tagcgattca gttagaacaa aaagcaacca agcaagaaat cttgacctac
    66301 tatataaata aggtctacat gtctaatggg aactatggaa tgcagacagc agctcaaaac
    66361 tactatggta aagacctcaa taatttaagt ttacctcagt tagccttgct ggctggaatg
    66421 cctcaggcac caaaccaata tgacccctat tcacatccag aagcagccca agaccgccga
    66481 aacttggtct tatctgaaat gaaaaatcaa ggctacatct ctgctgaaca gtatgagaaa
    66541 gcagtcaata caccaattac tgatggacta caaagtctca aatcagcaag taattaccct
    66601 gcttacatgg ataattacct caaggaagtc atcaatcaag tggaagaaga aacaggctat
    66661 aacctgctca caactgggat ggatgtctac acaaatgtag accaagaagc tcaaaaacat
    66721 ctgtgggata tttacaatac agacgaatac gttgcctatc cagacgatga attgcaagtc
    66781 gcttctacca ttgttgatgt ttctaacggt aaagtcattg cccagctagg agcacgccat
    66841 cagtcaagta atgtttcttt cggaattaac caagcagtag aaacaaaccg cgactgggga
    66901 tcaactatga aaccgatcac agactatgct cctgccttgg agtacggtgt ctacgattca
    66961 actgctgcta tcgttcacga tgagccctat aactaccctg ggacaaatac ccctgtttat
    67021 aactgggata ggggctactt tggcaacatc accttgcaat acgccctgca acaatcgcga
    67081 aacgtcccag ccgtggaaac tctaaacaag gtcggactca accgcgccaa gactttccta
    67141 aatggtctcg gaatcgacta cccaagtatt cactactcaa atgccatttc aagtaacaca
    67201 accgaatcag acaaaaaata tggagcaagt agtgaaaaga tggctgctgc ttacgctgcc
    67261 tttgcaaatg gtggaactta ctataaacca atgtatatcc ataaagtcgt ctttagtgat
    67321 gggagtgaaa aagagttctc taatgtcgga actcgtgcca tgaaggaaac gacagcctat
    67381 atgatgaccg acatgatgaa aacagtcttg acttatggaa ctggacgaaa tgcctatctt
    67441 gcttggctcc ctcaggctgg taaaacagga acctctaact atacagacga ggaaattgaa
    67501 aaccacatca agacctctca atttgtagca cctgatgaac tatttgctgg ctatacgcgt
    67561 aaatattcaa tggctgtatg gacaggctat tctaaccgtc tgacaccact tgtaggcaat
    67621 ggccttacgg tcgctgccaa agtttaccgc tctatgatga cctacctgtc tgaaggaagc
    67681 aatccagagg attggaatat accagagggg ctctacagaa atggagaatt cgtatttaaa
    67741 aatggtgctc gttctacgtg gaactcacct gctccacaac aacccccatc aactgaaagt
    67801 tcaagctcat catcagatag ttcaacttca cagtctagct caaccactcc aagcacaaat
    67861 aatagtacga ctaccaatcc taacaataat acgcaacaat caaatacaac ccctgatcaa
    67921 caaaatcaga atcctcaacc agcacaacca taacatttat cacccagatt tttctgggtg
    67981 attttgtcat agcactattg tatttatgtg cttaacaaaa gcctgagaag acatcttctc
    68041 aggctttttc tgtactataa acctcattgg aaaagataga gactgcttcc gtaatcttgt
    68101 taagaggttt tactaaatac tagtctttct tcgtttttac tacaaagaga gcagataggg
    68161 ctagactaac acttgctagg aagagggctg tgtcagattg actctcacct gttgctggca
    68221 agatttgttc aggtgtttct ggtctagcta tttcttgctt ataatctggc gtcttacctt
    68281 gattggaaac taaagagcta agtttaaact caggtttctc tacagttgga gcatctggtt
    68341 tcgtagcaga atcttttaat tggttgagat aatctgcaaa gtattccacc attctttccg
    68401 tcaagagatg gttatccgtc gcatatttca tgcttgcctg aacgctagct acttcctctt
    68461 gattgtcttt gtctgttaat ttctgagctt tagccaaagc tgcttcatag cctgacaagt
    68521 caagaggtgt ttctgctacc tgtggacgag taaagatgag ttccgctgca gattggtatt
    68581 tatcgccacc atctccgtaa gtcttagtac ctgtaaggac aattttctta gccttaattg
    68641 tcttaccaaa atcaatgtct tttggcttat tgttatctgg ccaatcagtt gcagtaaagg
    68701 tatgctcctt gccagactca tctgtcacaa caagtttcac atcacgcaag ttaccatttg
    68761 aacctgatcc acgtggaaca taacgaagtc cagtgatttc agttggttct ttcaagacca
    68821 tggttgcagg cttgcctaca tctcctccgc cccatgatgt atgccataaa ctagataagt
    68881 ttccatcaaa ggcatttgca agaccttctt gagcctgagc aggagctgta agactttcaa
    68941 agtcttctgc taccaaagcc gttttcttct gaaccaaagc attcttcaag gcttcaatct
    69001 tggcaatctc tgcacgcgct tcttccacac tgatatcatc atcggcctga ctgaggttaa
    69061 agaccgcctc tttcaaagca tccatagact ctttggtgta gttagtcatg gcaaccgttg
    69121 gcaagtagtt cttcagagca ttttctgtca acatcttacc tgttagggta atttcttcga
    69181 tttgaagatt atccatcatg aagtcgttat aaccacggaa gttggcattt ccaccagaat
    69241 caccacgagt attacttgca tttccagttg agtagatacc tacccaagta tcccctgttt
    69301 ctgcacctgt cacaaggaag gttgccttct tggctttctt agaatctgtc caagtatttg
    69361 gcaattcatg aatttccaag ttgcttgctt gagtgccacg acgacctgac tggaattctc
    69421 ccttaccgac tacaaaagaa taggtattgt ctgaacctgc ttcgtattca aaggttacac
    69481 ggtaggtctt acctgcttca aaacggaagt tttgcggaat agtttggtaa accaagttac
    69541 gacggctcac tagtccattt gtcttgagtg accaatttcc ttcgataaca tcatcgactt
    69601 ttttaccatt ccaaccacgt tgtgtatatg gatcgtgttt ttcagacaag tgagtacggt
    69661 tgtcttcgac accttcgaca ccacccacta caaatgggaa gataccctga gcaacatttt
    69721 caaagtcttg cttgaaggtg cctttacctg tatcatgctt gtctccatac atgcttgaat
    69781 tgttttcaaa ggtacgaatt tcatcaaagt aagttgcttg atcaccagct tcacgactca
    69841 atgtcagagt aacatttgag acgtccgatc ctgttgtaaa gaaggcgtac atgttttgga
    69901 agtaacttgt atcgttaact gtagcattgt tacgacgtgt attgtgggcg taggctttta
    69961 catagttgag ggcgagagac ttattggtat aagtagtcac ttctttttca ccagtattca
    70021 cagtgatact tgccttggca ttactacggt tatctacacc aacataaacg gcatacttgg
    70081 tatttggttt caagccagtt aatttctgag tgagactaac tttttctttg tttccttgaa
    70141 tacgaagcat atcgtttgcc ccttgagact tgacaatttc tgccttagaa gcatcgcctg
    70201 aaatggtcca atgtttcaag gtaccgctat taaatccttg gtcatagatg tgcatgcctt
    70261 cactccatga catttcagga ttagtttgtt tcgaacgata gagaacgtat ggttgatttg
    70321 ctagaagatc tagggtaatt ttaccatctt ttacagttag ttcttgctct tctgtcttac
    70381 cttggtcagt tagcttgtaa aggtaaacct tgctctttgc ccaatcgctt ggaagggtcc
    70441 aagttgttgc accggcctgc gtattgaagt agtacatctt ttccttatca gtagaaagtt
    70501 tcttaccatt tgcatcccag ttccaaggag tcaagtaagc tgaaccatct tggatgacac
    70561 gtccgttgag cgttactgta cgttcacggt atagtgggct attgacatca tttgacttac
    70621 gagttacaac taatttatta ttgtcagcat ctaccaactc cactcgcatt tctggagtcc
    70681 atttataagt gctaccgtta tcggtcatag tcaccggtgt accattttcc catttactta
    70741 cagtgaagtg ttggaagtac ttggtcatga cgtcatgagc aaataagttg gttacatagc
    70801 cattgtagtc acttcttcct tgccagcctt caaagtcttt catgctgtag ccacctagca
    70861 gtggatagtt ggctgcacca ccataacttc tgtagtcccc aacccaagca tctttttggt
    70921 ggttacggat aaagcgggtg atggcactgt tgataccttt attggtgtag ccaccgtagg
    70981 tcaagtcagc tgcccagtga tggaaggtag agtcgtactc accaccatgg ccccactcga
    71041 tcgcaaagcg ccagccttgt ttgttaattt ctttagcaag aacgtgggta gcccaggcac
    71101 cgttatcacc tgattgacca ttaccccaaa cgtccacata gataaagtcg agaccgtcac
    71161 caagtttttt cttcaaatct tcccaacgtg ccaaacgact atgagctagg tcataggcag
    71221 catcaatgtt gataccttga tctagccagt tccaaccata gctatagctt ccatctggat
    71281 tcttacggag aattttttca ttgaagtatt tagactcagg ataagtttct gaagcgttaa
    71341 cgtggatacc tagatgagct ccatatttct tagccttctc aattagggtc ttgaagtctt
    71401 cgacaccacc gatacgctta ccaatatcag catagttcaa gtgaccagag tcatggcctt
    71461 cgctaccata tcctttaagg agaacacctt gcccaagacc atctgtgtgg agattgattt
    71521 tcttgatacc atccaaggtc ataaggaatg gattttgtgc ttgagaacca aagttcatcg
    71581 cgatacggta agctgtgata tccttaactt tttcccaacc ttgaggattg ttcataatgc
    71641 tacgataagc aatggcacca tcttgccaat caacgttctt gtctgcattg gcatcttcag
    71701 tgataacaac cttagcactt ggaagttcct tcgtgtattc tgggaaaaca atgcccttat
    71761 aagctttttc ccattgccat tcagagctgt ggattcctac atagttggca tttccgactg
    71821 tttctttata agctgtcaaa cgagtccagt cattcgaacc accaccatag ctattttgag
    71881 agttactcca aacaccagca gcaagcttat ctgtagaaac aaatccatac atgtaaccct
    71941 tggctaggtc tttcattgga ttggttacat cgatatgatc atctccgctg acatgcgtat
    72001 tgtttgacat ggttgcccca tcaaacttag ctccagtttg atcactagaa acagagacta
    72061 aagcattgcc gaggaaacta atagaagaaa gtagttttct ttcgtcatca atcttttgac
    72121 ctggagtgac ttgattgtgg ttgacaatct tggtcacatc aaagtgcaat tgattgtcca
    72181 caacttgcaa gcgtactgtc atttccgcat tgattaagtg agcatcatcg cgaagcttca
    72241 tcaagtactc tgctgttgtc tcattgattt tcttataagt gacttcaggg gtgattcggt
    72301 ggttattgat aaagacttgg ttgaactgtt gcacctgtcc tggcaaagta tgcccgttca
    72361 agctgtattc cttgacacga gggaaggctt ggtcaatcac tgctttgagg accttagact
    72421 gaatcgtgtc ataagtcacc ttgctatcat caacttcagg acctgtttct ttttcagcag
    72481 gggtatcctc tgtttttacc ccctcttggt tatccgtttt aatgctgaca accgtacgct
    72541 catcgtcata agagcccgcc ttgagaagga tcttcttctc atttttaaga tggtcattga
    72601 ccgcagctgg tagagtcact gtgtcaaaga gattgacatc gttgttgctg gcatttagct
    72661 gaccgtctga cttgagagtg atagagagac ggtttgttga tcctgtttca ggagcagcaa
    72721 cacgactacc tctataccaa gtgctagctg ttggagattt atactcccag aaccagccat
    72781 ccttgtcata accgacaaaa acattattat tggtatcttt aaatttcaag aagacaccaa
    72841 agcgtgattt gcccttttca gaatcatctt tgaaggttaa atcaacagtt gcatttccat
    72901 tggcatcaac ggtcaagccc ttcttttcaa acagagctgg tttacctgcg ttatcatttt
    72961 gagcagttga ggatagttgg ttgtagcgga cacctttttc ttctcggata gtgactgttc
    73021 cctgttgttc ttttttctct accgtttgcc attcaggggt taccgtctta ggtgtttcag
    73081 gtttagcagc tgcaggttta tcctcttttg gtttttcttc agccggtttt tctttttcaa
    73141 gtgctaatag ttcttcttct gtcttaggtc catctgtaac ttctggagaa ccttggtctt
    73201 ctcccacctt aggcgcttca aaatcacgcc catcattctc ttttgctgtt gcaagagcag
    73261 tagctaaatc agctggtaag ttcgccgtgg aaccagactg cacgctatct gcaagaaccg
    73321 gacttgtccc aaagaatgca gctccaatca taacagaagc aacacctaat gaaaatttcc
    73381 gaatactata tttacaacgt ttttcaaata atcctttatt cataaacttc taaatccttt
    73441 catttttgag caagcgttta cattttcttt ttaaaaaata ctccttcctc tgaaagtgca
//
LOCUS       DBERCA010000005        68813 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.005, whole genome shotgun sequence.
ACCESSION   DBERCA010000005 DBERCA010000000
VERSION     DBERCA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 68813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.005"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..297)
                     /locus_tag="ACL719_000354"
     CDS             complement(1..297)
                     /locus_tag="ACL719_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698385.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="ACL719_000355"
     CDS             complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="ACL719_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGS0698386.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3571..4797)
                     /locus_tag="ACL719_000356"
     CDS             complement(3571..4797)
                     /locus_tag="ACL719_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGS0698387.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4840..5532)
                     /locus_tag="ACL719_000357"
     CDS             complement(4840..5532)
                     /locus_tag="ACL719_000357"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS0698388.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5705..7456)
                     /locus_tag="ACL719_000358"
     CDS             complement(5705..7456)
                     /locus_tag="ACL719_000358"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698389.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7446..9191)
                     /locus_tag="ACL719_000359"
     CDS             complement(7446..9191)
                     /locus_tag="ACL719_000359"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698390.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9319..9849)
                     /locus_tag="ACL719_000360"
     CDS             complement(9319..9849)
                     /locus_tag="ACL719_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGS0698391.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENRKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(9846..10302)
                     /locus_tag="ACL719_000361"
                     /pseudo
     CDS             complement(9846..10302)
                     /locus_tag="ACL719_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10436..11263
                     /locus_tag="ACL719_000362"
     CDS             10436..11263
                     /locus_tag="ACL719_000362"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGS0698392.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11275..11526
                     /locus_tag="ACL719_000363"
     CDS             11275..11526
                     /locus_tag="ACL719_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0698393.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            11533..11661
                     /locus_tag="ACL719_000364"
     CDS             11533..11661
                     /locus_tag="ACL719_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGS0698394.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     regulatory      12044..12139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12539..13120
                     /locus_tag="ACL719_000365"
     CDS             12539..13120
                     /locus_tag="ACL719_000365"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGS0698395.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13120..14382
                     /locus_tag="ACL719_000366"
     CDS             13120..14382
                     /locus_tag="ACL719_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGS0698396.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14469..15335)
                     /locus_tag="ACL719_000367"
     CDS             complement(14469..15335)
                     /locus_tag="ACL719_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HGS0698397.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15322..16092)
                     /locus_tag="ACL719_000368"
     CDS             complement(15322..16092)
                     /locus_tag="ACL719_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS0698398.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16118..16972)
                     /locus_tag="ACL719_000369"
     CDS             complement(16118..16972)
                     /locus_tag="ACL719_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGS0698399.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17129..17533)
                     /locus_tag="ACL719_000370"
     CDS             complement(17129..17533)
                     /locus_tag="ACL719_000370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGS0698400.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEDRQVRI"
     gene            complement(17603..19084)
                     /locus_tag="ACL719_000371"
     CDS             complement(17603..19084)
                     /locus_tag="ACL719_000371"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS0698401.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(19103..20281)
                     /locus_tag="ACL719_000372"
     CDS             complement(19103..20281)
                     /locus_tag="ACL719_000372"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGS0698402.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            20446..21453
                     /gene="galR"
                     /locus_tag="ACL719_000373"
     CDS             20446..21453
                     /gene="galR"
                     /locus_tag="ACL719_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGS0698403.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21499..22536)
                     /locus_tag="ACL719_000374"
     CDS             complement(21499..22536)
                     /locus_tag="ACL719_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGS0698404.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAVEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22537..22890)
                     /gene="nmlR"
                     /locus_tag="ACL719_000375"
     CDS             complement(22537..22890)
                     /gene="nmlR"
                     /locus_tag="ACL719_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGS0698405.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23134..24024)
                     /gene="czcD"
                     /locus_tag="ACL719_000376"
     CDS             complement(23134..24024)
                     /gene="czcD"
                     /locus_tag="ACL719_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGS0698406.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24157..24693
                     /gene="sczA"
                     /locus_tag="ACL719_000377"
     CDS             24157..24693
                     /gene="sczA"
                     /locus_tag="ACL719_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGS0698407.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24860..25666)
                     /gene="pnuC"
                     /locus_tag="ACL719_000378"
     CDS             complement(24860..25666)
                     /gene="pnuC"
                     /locus_tag="ACL719_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGS0698408.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26011..27531)
                     /locus_tag="ACL719_000379"
     CDS             complement(26011..27531)
                     /locus_tag="ACL719_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGS0698409.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(27524..28252)
                     /locus_tag="ACL719_000380"
     CDS             complement(27524..28252)
                     /locus_tag="ACL719_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698410.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(28267..29031)
                     /locus_tag="ACL719_000381"
     CDS             complement(28267..29031)
                     /locus_tag="ACL719_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS0698411.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29035..29478)
                     /locus_tag="ACL719_000382"
     CDS             complement(29035..29478)
                     /locus_tag="ACL719_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS0698412.1"
                     /translation="MRDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30160..30456)
                     /locus_tag="ACL719_000383"
     CDS             complement(30160..30456)
                     /locus_tag="ACL719_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS0698413.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLASFIKREESKYRGTATSALMYEELFKML"
     gene            complement(30478..31542)
                     /gene="pepA"
                     /locus_tag="ACL719_000384"
     CDS             complement(30478..31542)
                     /gene="pepA"
                     /locus_tag="ACL719_000384"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGS0698414.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            31942..32643
                     /locus_tag="ACL719_000385"
     CDS             31942..32643
                     /locus_tag="ACL719_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGS0698415.1"
                     /translation="MTKTILVTGETGYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKSYLEENQIDAALHCAGETVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNAAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            32659..32769
                     /locus_tag="ACL719_000386"
     CDS             32659..32769
                     /locus_tag="ACL719_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGS0698416.1"
                     /translation="MNYLFQEDSSSQIFNLETKKGYTIKEIFKTAEELLN"
     gene            33066..34025
                     /locus_tag="ACL719_000387"
     CDS             33066..34025
                     /locus_tag="ACL719_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698417.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            34015..34971
                     /locus_tag="ACL719_000388"
     CDS             34015..34971
                     /locus_tag="ACL719_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGS0698418.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            34968..35720
                     /locus_tag="ACL719_000389"
     CDS             34968..35720
                     /locus_tag="ACL719_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698419.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            35816..36781
                     /locus_tag="ACL719_000390"
     CDS             35816..36781
                     /locus_tag="ACL719_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698420.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIIGMPTKTIPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSIKANIES
                     LASAFGETSIQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(37006..37248)
                     /gene="yidD"
                     /locus_tag="ACL719_000391"
     CDS             complement(37006..37248)
                     /gene="yidD"
                     /locus_tag="ACL719_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGS0698421.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(37248..37970)
                     /locus_tag="ACL719_000392"
     CDS             complement(37248..37970)
                     /locus_tag="ACL719_000392"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0698422.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(37957..38526)
                     /gene="scpB"
                     /locus_tag="ACL719_000393"
     CDS             complement(37957..38526)
                     /gene="scpB"
                     /locus_tag="ACL719_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGS0698423.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(38545..39273)
                     /locus_tag="ACL719_000394"
     CDS             complement(38545..39273)
                     /locus_tag="ACL719_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGS0698424.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(39273..40007)
                     /gene="xerD"
                     /locus_tag="ACL719_000395"
     CDS             complement(39273..40007)
                     /gene="xerD"
                     /locus_tag="ACL719_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGS0698425.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(40004..40465)
                     /gene="cbpB"
                     /locus_tag="ACL719_000396"
     CDS             complement(40004..40465)
                     /gene="cbpB"
                     /locus_tag="ACL719_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGS0698426.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(40462..40983)
                     /locus_tag="ACL719_000397"
     CDS             complement(40462..40983)
                     /locus_tag="ACL719_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS0698427.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(40959..41930)
                     /locus_tag="ACL719_000398"
     CDS             complement(40959..41930)
                     /locus_tag="ACL719_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGS0698428.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(41927..42721)
                     /gene="racE"
                     /locus_tag="ACL719_000399"
     CDS             complement(41927..42721)
                     /gene="racE"
                     /locus_tag="ACL719_000399"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGS0698429.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(42928..43176)
                     /locus_tag="ACL719_000400"
     CDS             complement(42928..43176)
                     /locus_tag="ACL719_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGS0698430.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(43432..45057)
                     /gene="treC"
                     /locus_tag="ACL719_000401"
     CDS             complement(43432..45057)
                     /gene="treC"
                     /locus_tag="ACL719_000401"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGS0698431.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(45235..47202)
                     /gene="treP"
                     /locus_tag="ACL719_000402"
     CDS             complement(45235..47202)
                     /gene="treP"
                     /locus_tag="ACL719_000402"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGS0698432.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQVQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            47387..48097
                     /gene="treR"
                     /locus_tag="ACL719_000403"
     CDS             47387..48097
                     /gene="treR"
                     /locus_tag="ACL719_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGS0698433.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYIVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(48130..48933)
                     /locus_tag="ACL719_000404"
     CDS             complement(48130..48933)
                     /locus_tag="ACL719_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698434.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(48946..49823)
                     /locus_tag="ACL719_000405"
                     /pseudo
     CDS             complement(48946..49823)
                     /locus_tag="ACL719_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(49835..50772)
                     /locus_tag="ACL719_000406"
                     /pseudo
     CDS             complement(49835..50772)
                     /locus_tag="ACL719_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(51263..52189)
                     /locus_tag="ACL719_000407"
     CDS             complement(51263..52189)
                     /locus_tag="ACL719_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698435.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(52200..53267)
                     /locus_tag="ACL719_000408"
     CDS             complement(52200..53267)
                     /locus_tag="ACL719_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698436.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYAPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(53276..54202)
                     /gene="oppC"
                     /locus_tag="ACL719_000409"
     CDS             complement(53276..54202)
                     /gene="oppC"
                     /locus_tag="ACL719_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGS0698437.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(54202..55698)
                     /locus_tag="ACL719_000410"
     CDS             complement(54202..55698)
                     /locus_tag="ACL719_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698438.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(55765..57744)
                     /locus_tag="ACL719_000411"
     CDS             complement(55765..57744)
                     /locus_tag="ACL719_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698439.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(58430..59623)
                     /locus_tag="ACL719_000412"
     CDS             complement(58430..59623)
                     /locus_tag="ACL719_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS0698440.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(59761..61203)
                     /gene="gtfA"
                     /locus_tag="ACL719_000413"
     CDS             complement(59761..61203)
                     /gene="gtfA"
                     /locus_tag="ACL719_000413"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGS0698441.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(61514..62347)
                     /locus_tag="ACL719_000414"
     CDS             complement(61514..62347)
                     /locus_tag="ACL719_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698442.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(62361..63227)
                     /locus_tag="ACL719_000415"
     CDS             complement(62361..63227)
                     /locus_tag="ACL719_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698443.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIVMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(63241..64499)
                     /locus_tag="ACL719_000416"
                     /pseudo
     CDS             complement(63241..64499)
                     /locus_tag="ACL719_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(64534..66696)
                     /locus_tag="ACL719_000417"
     CDS             complement(64534..66696)
                     /locus_tag="ACL719_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS0698444.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQIEKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYENQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            66804..67664
                     /locus_tag="ACL719_000418"
     CDS             66804..67664
                     /locus_tag="ACL719_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS0698445.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHMGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            67661..68596
                     /gene="birA"
                     /locus_tag="ACL719_000419"
     CDS             67661..68596
                     /gene="birA"
                     /locus_tag="ACL719_000419"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGS0698446.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(68722..68809)
                     /locus_tag="ACL719_000420"
     tRNA            complement(68722..68809)
                     /locus_tag="ACL719_000420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68773..68775),aa:Ser,seq:gct)
BASE COUNT        20666 a        15338 c        12547 g        20262 t
ORIGIN      
        1 ttatttcatt ttattatact ctgttaattc atatgagttt aaaccgattt catctttaac
       61 ctcgagtaaa gcagtttcaa atatttgttt aagagttttt gattctttac aattaaccga
      121 caaactttct gataaaatat gtacaacttc tgagactgaa taacctatct cctctttaga
      181 attatataaa tctgtagctc caccaataat ccaaaaatac tgattttgtg aacctacaat
      241 atcctcattt tctacggaac ttccttgtat cgaacaaatt ttatttatct ttaccataat
      301 acttcaaccc ttttagtgtc aaaagtaaac caattcctgt cactgttaag aatagttcca
      361 taatcttgtt cgaaccagtc tttggtaatt tttgttttac atctactatt tctttagatt
      421 tattaatatg attttcagtt tctctgccat ctccaactat tttatagttt acttcttctg
      481 tcttattatc ttgtttattg tcgatcttgt cattcatttg tctattatct ttacttgagt
      541 taaactctcc gttcttctgg ttactatcaa ttacattatt tgaattagat tgtttttcct
      601 ctttgttttt ttctttttcg tttttatcac ttaaattatt tgttacaatt ttgtaaagcc
      661 cattctccgt tacaatattg aaattaccat cgctatcacg tataacaggt tctttcccgt
      721 ttgcattaga tttgatgaat gatatatact taccggataa attataaaat tggttattta
      781 aaacggttat tttacccttt gaatcctcaa taacaattcc ttctttacca ccgaatatac
      841 tattctctga cactactaaa ttatcactaa ctcgtaataa ttcaatattt ggttgttctg
      901 tttccttaga attattatta attacatttt ttgttacagt aatatctgat acttttccta
      961 aatattctgc actatctttt gcaactcgta tcgcttttgt tttaggatcg gcaatattaa
     1021 atatattttc ggcgataacc acctgtttat ttaaatctaa taggtcttta gtgtttttat
     1081 agctataagc atttactaaa gctgctccgc tttctcgata atgtacactc tctcctttaa
     1141 ctttcttatc aaagcgattt cctttgatta atacatcagt gaatcctgta aaacgtacac
     1201 ctgcatacat catgttatca aaatgattat ttaaaatttt aatattagag gggttctgtg
     1261 tcgacaatgt ttgatagtgt gtgccaattg ctgttactaa ttccccagat ttatcacttt
     1321 tgccaaaata ggaattttga atagtcacat tttcagattt tttcccatca tcattcaagg
     1381 cataaggaaa accttttcta gttaatggtt caatctgaat gctctcctta cttatgattt
     1441 gcccatcctt catcgttttg ggtaaggctt gcccaagaaa acgagaatta tcaactaata
     1501 catttttcga acctgcaatt tgaatagcat gcccttgata actatccttg aatgttacat
     1561 tttttatagt tacgttattt gaattcccaa tagcaaatgc acctgaagaa tttataagtg
     1621 gtagattttt tgctttagtt ccttcttcat tcaaagcacc gttcatatca atcgtaccac
     1681 cagaataact aatatcttct gttgggcccc attctacttg cgcaccatca tccgtaaata
     1741 aacctgtcat aaaaacaatg gaagggtgat tcttaatatt tataccattt agaattgtag
     1801 ctttctcatt caattctaag tgtgtatgac tttttaaaaa aacaatttct tttactaaat
     1861 aagttccttc aggaaaatat acatttcctc cacctagccc ttgagctgca gcatctattg
     1921 catcttgaat tgcttgtcga tcatcatgaa tcccatcacc taccgcacca taatctttta
     1981 cacttaaagc agtatcttca aattcttttt tgataattct gtcacttata acatcttcct
     2041 tataactctt attttcatta gtagatgtta ctgtagattc aactgttctg ttgtctaatt
     2101 gtgaattgga acgaatagta ccattagagc tgatactatt aacacttaac gaattatcta
     2161 aaccattagt ggttgaatta gaggcaatca tttcagcaga acgattctga ttaacagttg
     2221 gtgaattatc taaatcatta gtaattgcat tagaagactc ttttgtagcc atcggtgaag
     2281 aactagtaac aacttcatcc gataaaatac cttggcttcc caaaattgaa attgccaata
     2341 gtaccgagca agtccccacc tttaatttac gaatactgaa tacctcatta ggaacaaaat
     2401 atttcatcag aacctccaat ttttttacta tttttaacaa ttgattatta cttttcttaa
     2461 aatagataga atgacaccgc tgtcattatc tttatcatat attttccaaa ttgaattgtc
     2521 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
     2581 cagtgccgcc ttgccctaag tctaggttac tgacttggtc agttctatag tagactgaaa
     2641 ctataatagt acacctctac ttctaaaaca ttgttagaaa tcgatttaac tgtcccgatc
     2701 aatttgtcct attcttgttt cattttacta tatctacaac ctcaaagcag ggttttgagc
     2761 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
     2821 tcttagcttg aatataaaat atcccattta ctatatttaa atttgttgat atgttgtaaa
     2881 ttataaatga aagaacaaat accttgtcat acaagcattc gccagggata tttacctgaa
     2941 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
     3001 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
     3061 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcaggag atctaatgat
     3121 gtatagcaag catcactaaa catatgggat atttgcgtta ttataatatc aacatgctat
     3181 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
     3241 tattctgaaa acaaaaaaaa gacgctagtt tccacaatct cactcatgga aactcacgtc
     3301 tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc
     3361 tagtaaggat tctaaatgct ataccaaatt gctcacatct tatttcaaac cacgggactc
     3421 ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg cctcaactac
     3481 aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg
     3541 acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc
     3601 agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg
     3661 aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc
     3721 aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc
     3781 aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc ttgctccttc
     3841 tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg
     3901 atggatgcga gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg
     3961 caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat
     4021 gttgcactta taggctggtg taacgatatc gtattcccat gaccccagtt gcatcttggc
     4081 aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc
     4141 gtcatcaatc actggattgg ctttccaagt cgcacttcca ccttctgccg ttgtaaagtt
     4201 cttaacagca tggaatgaga aggaagtaaa atcagcgata gaaccagaag gttgtccttt
     4261 ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt
     4321 ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg
     4381 gtcataatcg caaacaatcc ctgcgagctc tactgggata atcaccttag ttttctcagt
     4441 gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat
     4501 gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg
     4561 aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcggc
     4621 tgtcgcagag ttgagacaaa cagtcttagg tgtctgtgtg taaagagaca agcggcgctc
     4681 cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc
     4741 agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga
     4801 tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac
     4861 ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt
     4921 atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt
     4981 ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt
     5041 aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct ctgtataacg
     5101 tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg
     5161 aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc
     5221 gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacggaactt
     5281 ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc
     5341 cttgctatcc aacttgatcc aaatgctcaa gatgagaaag atgggagaag ttagaaccag
     5401 taagaccaag gccaaaaccc agtccaagca acgcttgaaa atcagcgaac ccttcctttt
     5461 agagacaagc tggtagtaag actcaacctc gcttgatttc atttccacag gcaagtcttc
     5521 ccatttcagc atgctgttct cctttgtttt tattatacta tttgtcaaca tttttcatta
     5581 taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta
     5641 ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag
     5701 attttcaaag agtataggcc atggcccctg cctgcaaact atacatcttg tgataggttc
     5761 ctcccagagt caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga
     5821 catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc
     5881 ggccctgtct catcttcgcc agagaagctt gaaccaaact ttctgtttca gagtcaatat
     5941 tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg
     6001 caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggagagtcgt
     6061 acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa
     6121 cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga
     6181 ggaagggttc ctgtaaaacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc
     6241 tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga
     6301 cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct
     6361 tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatac gagaaacaca
     6421 catgttcaaa acggatattg ccttctttga ctttggcttg cccattttct tgaaggggct
     6481 cataggtcct ctcatcgatt aaggcaaaga cacggcctgc agaaaccata gacgtttgca
     6541 gtgttgaaaa gttttgcgtc acctcaatca aagggtcaaa gaggcggttg atgtactgga
     6601 taaaagcata catggtcccg gccgttatcc cgatagaaag accacgatag ccaaaatagg
     6661 tcatcaagac agcataacct agaagtttca acaaactcat ggcaggtctc aaaaagaggg
     6721 catccaaggc tacagaacgg ttggcatagg ccaagtgctc ttggttgatt tcatcaaatt
     6781 ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc tcgatattct
     6841 ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg attttcactg
     6901 acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg
     6961 ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag ataaaaactg
     7021 ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg gtatcgttgg
     7081 tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct
     7141 ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa
     7201 agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca
     7261 gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata aagtgggaag
     7321 ccacaagggg aatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa
     7381 aggtaaggag tccataaggc ttgagatagg acatcaagcg cttcaataca gtccattgtt
     7441 cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg gtaggtttgg
     7501 gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca
     7561 ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg ggcagtgata
     7621 atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg
     7681 gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa aatcatagcc
     7741 cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc
     7801 agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta
     7861 gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt
     7921 gaagtcgcaa aaagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc
     7981 agacgatagt cccgaatatc gtgaccgttt agataaatgg cgcccttatc cacatcgtat
     8041 tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc
     8101 agtgtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa
     8161 gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactcc
     8221 gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc
     8281 cctcgctgag tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc
     8341 aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg aaccaccaag
     8401 gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac catagggtca
     8461 aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc attgactgcc
     8521 tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt gatacctgat
     8581 acggactcct gtaccttgtt attgagttca gaaaaagcag cttgggattc gccaaaggcc
     8641 ttatgagtct ttctccctag gcgactagtc gcataggcca tgaaaggtag ggggagaatg
     8701 gcaacaagag tcatctgcca tgagatgcta aagagcatgg tcaacaaagt caccagagcc
     8761 gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt caaggcattg
     8821 atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata aaaggctgac
     8881 gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa gcaataagag
     8941 gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa ggctgcaagt
     9001 agcaagtaaa ataggctaag aaggaggtcc tgctgggtta attgccccga tgtgatggca
     9061 tcaataaccc gccccataac cataggagga atgagattga ggacggaaac caagaccagg
     9121 gccacaattc cgactagata acggcgtttt tctaacttga aaaaccacca aagtttttga
     9181 ataatggaca taaaatccct ttctgattgc aaatggaaac ctgaggccaa tactcaatga
     9241 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc tttgaggttg
     9301 tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga cgcggtttga
     9361 agagattttc gaagagtaca agaccccagg tttttctttt tcataagtta cgactgtaac
     9421 agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc cttgtttact
     9481 gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat ctacttgaat
     9541 cacaccgtca gtcacaactg gatacttacg attttcatct cccatttgca caacgataag
     9601 ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa tcccttggct
     9661 acgaagtttg agggctacat ccgatgcaga caaaccaact gaacgacagg cttctgggtc
     9721 aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt tcacaaagtg
     9781 aacattgttg ttgagccatt tgagagtcaa gaccagtttg attaaaattt cgatataatt
     9841 aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa ttcgactttc
     9901 tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg atctcccacc
     9961 ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga cttgtctact
    10021 cccaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt attgttagaa
    10081 acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata aggccagagc
    10141 tgaaatcatg accaactcac gaaattcggt tcccttttta tcatggtatg ccttgaaagc
    10201 aaaaaaaact gtcacagcta atagaacaat cccaaagcca accatgatac cattttgctg
    10261 accgatttgg ctgagtacat agtcataaga gtagaatttc atatattttc actctctttc
    10321 ataaaatcat tcttaattat aaacgaaaga gagtaatttt tcaaaccata cgttggggaa
    10381 atagatcttt ttttggtata ataaaatcta taatctgaat gaaaaaggta actttatgaa
    10441 acttatctca tggaatattg attccctaaa cgctgcccta actagtgact cagctcgtgc
    10501 caaattgtcc caagaagtcc tacaaacctt ggtcgctgaa aatgctgata ttattgctat
    10561 ccaagaaacc aagctttctg ccaaaggacc tacaaagaaa cacgtggaaa ttttagaaga
    10621 actcttccca ggctacgaaa acacgtggcg ctcttcccaa gagcctgccc gtaaaggcta
    10681 tgctggaacc atgttccttt ataagaaaga acttacgcct acgatcagct tcccagaaat
    10741 cggtgcccct tctaccatgg acttggaagg ccgtatcatc actctagaat ttgatgcatt
    10801 tttcgtaacc caagtttaca ctccaaacgc tggtgacggt ctcaaacgct tggaagaacg
    10861 ccaagtctgg gatgttaaat atgctgagta tttggctgaa ctagacaaag aaaaaccagt
    10921 ccttgcgacc ggtgactaca acgtagccca caatgaaatc gaccttgcaa atcctgctag
    10981 caaccgccgt tcacctggat ttactgacga ggaacgtgct ggatttacca accttttggc
    11041 aactggattt acagacacct tccgccatgt tcatggcgat gttcctgaac gctacacttg
    11101 gtgggcacaa cgcagcaaaa cttctaaaat caacaataca ggctggagaa tcgactactg
    11161 gctcacaagt aaccgtatcg ctgacaaggt cactaaatct gatatgattg actcaggtgc
    11221 tcgccaagac catacaccga ttgtcttgga aattgacttg taaggatttt ctatatggac
    11281 tacaatgcgg tcattcccga gttgcttgta tcgaatatcg aacagtctcg gtccttctac
    11341 tgtggtttac tgggttttag gattgaatac caacgtccag aagaaaactt tctctttctt
    11401 tctcttgaag agtgtcaact aatgttagag gagggaacaa aggatcaact agctgaactg
    11461 acctacccct ttggtcgtgg agtcaatctc tcctttggca taaaggatgt ttcaaaactc
    11521 tattaaagag tgatgaaggc aaactatcct atttatcgtc ccttgaccaa aagaaagttt
    11581 cgtgtcagtg atccctatat ctatcctcat aaatttgcag ttttggatcc aaatggctat
    11641 tttttaagat ttagcgagta gaataataaa aattgggaaa agtctttaaa gttagaaaaa
    11701 ggcatattgt caggtgttta aaaacttgat agtatgcctt ttatgatgga aaaatcttct
    11761 aatactcttc gaaaatctct tcaagccaca tcaacgtcgc cttaccgtag gtatatgata
    11821 ctgactctgt caattctatc cacaacctca aaacagtgat ttgaggttgt ggatagcttc
    11881 ctagtttgct ctttgatttt catttagtat aaatgttagc ttacaatgca gtttttgttc
    11941 actccttctt ctgtttgttc gttttttaaa ccaaaatact aacaaaatag ttgatttata
    12001 aagttattta gtgtaaaata aaaacattgg ctcaaaccta tttaaaattg aatatcgttt
    12061 tacttgttta tgtcgtgaat tggcacgacg tttctacaag gtgccggaac acctaacaat
    12121 aagtaagtca gcagtgagat atggccgttt ttgccatgcc tatttggggg acttacttag
    12181 agattaagta ggtctttttt gaatattttt aatatttccc ttaattagtg cggacgggag
    12241 gtgaaattat cctgtgaata atttcaggaa tccagggaat ttcatcccta atttttgagc
    12301 ccctagctct ttcatttcta ggcttaggct aaaatagttc cccgaactat tttactctca
    12361 aaaatcccgt catagacaat aacaagatta ttgtctatga taccactaca acgggaaata
    12421 tttgataaga ctcgcttcgc tcgtaatagt tttatcaaat atatagtatt agcaaaaggg
    12481 atcactatag aaacatttaa acaacaagta aattaacttt tagactttag gaggtcttat
    12541 gaaattatta gaagagcgca tcctcaagga tgggcatatc ttgggtgata acatcctcaa
    12601 ggtagattcc tttttaaccc accaagttga ctttagcttg atgcgagaga ttggtaaggt
    12661 ttttgcggaa aaatttgctg ctgctggcat taccaaggtc gtaaccattg aagcgtcggg
    12721 tattgcccca gccgttttta cagctgaagc cttaaacgtt cccatgattt tcgccaaaaa
    12781 agctaagaac atcaccatga acgaaggcat cttaactgct caagtctact cctttaccaa
    12841 gcaggtgacc agcactgttt ctatcgctgg aaaattcctc tcaccagagg acaaggtttt
    12901 gattatcgac gatttccttg ctaatggcca agctgctaaa ggcttgattc aaatcatcga
    12961 acaggccggt gccacagtcc aagctatcgg tatcgtgatt gagaaatcct tccaagatgg
    13021 tcgtgatttg cttgaaaaag caggctaccc tgtcctatca cttgctcgct tggatcgttt
    13081 tgaaaatggt caggtcgtat ttaaggaggc agatctctaa tgcaaactca agaaaaacac
    13141 tcgcaagcag ccgttctggg cttgcagcac ttactagcca tgtactcagg atctatcctg
    13201 gttcccatca tgattgcgac agcccttggc tattcagctg agcagttgac ctacctgatt
    13261 tctacagata tcttcatgtg tggggtggca accttcctcc aactccaact caacaaatac
    13321 tttgggattg gactcccagt cgttcttgga gttgcattcc agtcggtcgc tcccttgatt
    13381 atgattgggc aaagccatgg tagtggcgct atgtttggtg cccttatcgc atctgggatt
    13441 tacgtggttc ttgtttcagg catcttctca aaagtagcca atctcttccc atctatcgta
    13501 acaggatctg ttattaccac gattggttta accttgatcc ctgtcgctat tggaaatatg
    13561 ggaaataacg ttccagagcc aactggtcaa agtctcttgc ttgcagctat tactgttctg
    13621 attatcctct tgatcaacat ctttaccaaa ggatttatca agtctatctc tattttgatt
    13681 ggtctggttg ttggaactgc cattgctgct actatgggct tggtggactt ctctcctgtt
    13741 gcggtagctc cacttgtcca tgtcccaact ccactctact ttgggatgcc aacctttgaa
    13801 atctcatcta ttgccatgat gtgtatcatc gcaacggtgt ctatggttga gtcaactggt
    13861 gtttatctag ccttgtctga tatcacaaaa gatccaatcg acagcacgcg ccttcgcaac
    13921 ggttaccgcg cagaaggttt ggcggtactt ctcggaggaa tctttaacac cttcccttac
    13981 accggatttt cacaaaacgt tggtttggtt aaattgtcag gcatcaaaaa acgcctgcca
    14041 atctactacg cagctggttt cctggttctc cttggactgc ttcctaagtt tggtgccctt
    14101 gcccaaatca ttccaagctc cgtcctcggc ggtgccatgc tggtgatgtt tggttttgta
    14161 tctattcaag ggatgcaaat cctcgcccga gttgactttg ctaacaatga acacaacttc
    14221 cttatcgcag ctgtttcaat cgctgcaggt gtcggtctca acaacagtaa tctctttgtc
    14281 agcatgccga cagccttcca aatgttcttc tcaaacggaa tcgtcgtagc cagcctactc
    14341 gccattgtcc tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga ggtgcgagcc
    14401 tcttttttgt ttatcacaaa ttgattataa atacaatcaa tcccaattcc catctaattg
    14461 aattggacct atttcaaatt ctttaaaata ttatgttcca aatcgttaat attatataag
    14521 aatggtaaaa tatcaaatgt ttctcgtaaa ttattcttag ccttatacca accttgtcca
    14581 tcagtaatcc acataaactc aacattagga atagctttag tttcttcagc aatcattttg
    14641 taagatctag ccgtctcatt taattttgaa ccacttcctg aataaaaatt aacctcaatc
    14701 agataaagca cttgttcatt ttttatgaca aaatcaaaac gtttcacagt attcccatca
    14761 ttcgtaatag cagataaatc gacagaaaaa atttcctcta tttcattttg ttcaatctct
    14821 ttaaataaat tctctcctaa aatataccct tcagcttcaa gatagctttg aacaatattt
    14881 tccatcgcat ctccagtccg atttttgcgc ccattagtat ccataccaac ctcaacacct
    14941 gtaacgtagt caactaaatt tgatactaaa tgattttgca ataaatcaaa tatacctgat
    15001 ttttccaaaa acatagtata ttcttcaata ctatagtttc gtttactaaa atcaaaatag
    15061 aaatctttta tcggatcctt aacaataatc gtatctctca aacgtttagc aataagtaga
    15121 ggcacaactt tcaaaatttc tggataattt tgatataagt ctaaaaaatc ctcttgtata
    15181 tttttactac caataagact attcataata tttagagcaa cttttatact agatacattt
    15241 ttataaacct taggaaaatc tgtataataa gtccagtcag ctactgtgtc ggtcatcgtt
    15301 gacaaccact catcaaaatt tctagtttgt ttcattttct gcctccaacc tcttccttgc
    15361 aatttttaaa tattcttttt cagcatcgat acctataaat cttctaccta accgcttcgc
    15421 aacaacaccc gtagtgccac taccaacaaa tgggtctaga atatagtcac cctctttagt
    15481 agaggctaaa ataatacgtt ctaacaaata ctctggtttt tgagttggat gtttcccagc
    15541 ccatttttca actttctttg ttaaagaacc ggtccagaca tctttcattt gttttccatc
    15601 attcaattct ttcattaaat cataattgta gtaatgacga gcttttttat cattttttct
    15661 ggcccataaa atggtttcag tagaatgggt aaaataacga caagataaat tgggggcagg
    15721 gtttgttttc tgccaagtaa tattattcag aattttaaaa ccttcttgtt ctaatgccat
    15781 tccaactgag tatatgttgt gcaaactacc tgaaatccat accgtcccat taggcttcag
    15841 aacttctttt gctaggcgaa tccatttacg attaaactca tgtttttctt cgaatgaaga
    15901 aattttatcc caatctcctt tatcaacaga aactacttgt cccccagaat tagatattcc
    15961 accattactt aaaaaataag gtggatcagc aaaaatcata tccatacttt ctggtttcat
    16021 ttttgataag aacttgaacg tatctgaatg aacaagaatc atcttatttt tattgtagta
    16081 tggttttgtc ataagaacac ctccatactt atattcgtta tttttcataa tttgtgacta
    16141 taatttcaga aatttttcct cgacttgaag attttgctcc attagttcgg gtagcttcaa
    16201 cataatgtat attaaaatcc ttatacaact cctctactaa agcactagaa gaatttgata
    16261 acataacata agctcctgta tcactcaatc tcttaaaggc atctcttaat cttacttgat
    16321 ctgcaaaaga gaatccctca tgagtataag acgtaaatgc actcgtctca gacaatggaa
    16381 tatatggagg gtcaaaatac acaaaatctc ctgttcgaac atctacaatt gccttttcaa
    16441 aatctcccac tttaatttct agttgattgt tatttatata aactgaaata gcagatatca
    16501 attcctcatc aacaatttta ggattcttat aacgtccata tggaacatta aattgattct
    16561 tagaattcac acgatataga ccattaaagt tcactctcaa catatataga atacgtgcag
    16621 ctctttgtac ttcggacatc atatctattc tttcatcacg atctgcagaa cgtaaatcta
    16681 aataatattc ttttgaatta tattcctgat gaactttcaa aatttcaatc aattcttgag
    16741 gattgtcctt aatttgttga tagcaattta ttagttcagc gttaaaatca ttaataactg
    16801 catctttagg agccaaatca aaaaataaag ctccacctcc aacaaaaggt tcgaaatacc
    16861 tgttataggt tttaggtatt aattctctaa taacaggcaa taattgtctt ttaccacctg
    16921 tccatttcgt gaacggttgt aaagtaactt tctttatttc ttttatcttc atgcttttaa
    16981 ttataccata gaaattaaaa cacaactctt gtattttcta ccacattact aaattttcta
    17041 ttttagaatt aaatcatatc aatatcaaaa aatgagtaag gcaaatctta taaattccaa
    17101 aattcacctc actctattaa attactcttt atatccttac ctgtctgtct tctgaccgct
    17161 ggcctgtgac aaacatggta aaggttgctt tgcagacatt tctgccttct tgattggtga
    17221 tatccacatc cattacacag gttgtacgac cttgatggac acattctcct ttaatggtca
    17281 atacatcgtc gagttttcct gccttaaggt agttgataga ggattggagt gtcaccccat
    17341 caagtcccag cgagataacc accaaaccac tgatttggtc acatagcgtg aatagatagc
    17401 caccatgggc attgccatag tagttgagcg acgagttcac tactttggtc gtcaccacaa
    17461 cgtgaccatc tctcattttt tcaatttcgt aattttcaaa ggcagatata gcgtcaaaat
    17521 gaaagtcttt catcgtttcc tcctgatatt tcaaacgaca ctatgatact actttttata
    17581 ggactgtgca aggagaaagc tcctagtcta gtaaaattcc gacctgttcc acaaagcgca
    17641 taaaggctgt ctgaccttgt tctgtctgct tgtagactcc tgcatcctca agtacacgca
    17701 caaagatagc acccacagag tccttgacga ttgcaagggc tttttcttta tccgttaggt
    17761 ctggatgttg ggatttgagt tggtctgccc actcctgatg ataatcggca actgtaacag
    17821 cttctcctac aagatagcta gcgacttgct ccacttcttc tttcagacgt ggtggcaaga
    17881 ttgccaagcc catgacctca atcaagccga tattttcctt cttgatatgt tggacatcct
    17941 tgtggggatg atagatgcca tcaggatgct ctgctgaagt ctgattgtct cgcaagacca
    18001 agtccaactc aaactgtcca tcacgcttac gcgctattgg tgtgatggtg tgatgcggtg
    18061 tcccgtctgt ctctgctaaa atctgcacac tagaatctga atactgacgc cattcctgga
    18121 aaatcttatc agctaagtta atcaaatcct ctttggaatc cgaagtcaag cgtaggacag
    18181 acattggcca cttgataatt ccagccttga cctgctcaaa accagcaaat cggaaggtct
    18241 tttgcaaggg agccaattcc ataggaaata cgtgacggcc tccctgataa tgatcatgag
    18301 ttagaataga gccccccaca atcggcaggt cggcattaga tccagcaaaa tatcctggaa
    18361 actgctctac gatagccaac agacgttcaa aactctgacg actaatggcc atgggacgat
    18421 gctggccatc taaaaagata caatgctcat taaagtaagc atagggcgaa tactggaacc
    18481 cccattcctg accaaccatt tcaaaacgga taatccggtg gttgctgcga gctgggtggt
    18541 tgacccgacc atggtaaccc tcattctcta aacaaagttg acactgagga taattactag
    18601 cttgcaccaa cttggctacc gcaatctctt tcggatcctt ttcaggctta gagagattga
    18661 tggtaatttc aagttctcca taatcagatg gaacacgata agcgatattt ttagcaatgg
    18721 ccttgagttt gatgtagtca tttttccgac tgagttgata aaaatcctct atcgcttgct
    18781 caggagagta ggcataggct tcccagaagt cacgattgac ctgactcgga caaggagtca
    18841 ccaagtccat cagttcagta ccaaggattt cacgcgcagt ctgactatcc tcaatcatct
    18901 ctaatcgaac ggcttcctca actagctggt ccttgaggtc aatcaattta tccagattgg
    18961 tctcaacttc caaaacacct tctcccactc gtgccaaaac acgattggtc aggtagattc
    19021 gatccatttc ctcaaatgaa ctttcagaaa tgacatgtgt tacaaattta tctactaagg
    19081 tcactataga gcctcctttt gactagtcaa ggacgcgagt cccacctgca acttcagcga
    19141 tatagaagct tggagcgtat ccaactactt cctcgtagtg tttgcctaca gcttccttaa
    19201 aggcctcaac agtatctttt tgaaccaagg caatggcaca gccaccaaaa ccagcccctg
    19261 tcatacgagc accgagaact ccttcttgtg cccaagctgt gtgaacaaga gtatccaatt
    19321 ccaaaccagt tacttcataa tcatgctcca gagaaacgtg tgacgcattc atcaaacgac
    19381 caaatgtttc caaatcacct gcttgaaggg ctgcttgagc tttaagggta cgttggtttt
    19441 caagcacagc atggcgagca cgtttcaaac gattttcatc tttaatcaaa tagctatatt
    19501 ggtcaacggc ccactcgtcc aattcaccca gggtctgaat atccaaggca acttgcaatt
    19561 cttccactgc tttttcacac tcagcacgac gttcattgta tttagagtcc gccaattcac
    19621 ggcgtttgtt ggtgttcatg ataacaacga cattgtcctt caaatcaagt ggcaccaaat
    19681 catactctaa ggtgttggta tcaaggtaaa tagcacgttg atcagccccc ataccaatag
    19741 caaactggtc catgatacca gagttgactc cgataaagtt attttctgtt tgttttccga
    19801 ttttaaccaa atcgagacgc tctaatttta aatcaaagag atgctctgcc actacccctg
    19861 tcaagagctc caaggatgct gaagaagaca aaccagcacc atttgggata ttcccataaa
    19921 cataaaaatc aaaacctttg tcaatcacgt gcccagcttc ttgcaagaaa tgaaggacac
    19981 cttttggata attggtccag ttgtgctctt tttcaaactt gaggtcagcg agaggcactt
    20041 cgataatgcc cttgtcctca aagttagctg agtagaaacg caagacttgg tcatcacgct
    20101 tacgagctgc accgtaagtt cccaaggaaa tagcagcagg aaaaacgtgc ccaccgttgt
    20161 agtctgtgtg ttcaccaatc aaattaatac ggcctggtga aaagaaggtt tggtctgctt
    20221 cttgaccaaa aacagcaaga aagtctttgc gaaggacttc agtagtaaga tgttgtgcca
    20281 tatgaattct cctttgactg tccgttaagc catcttaacg tgtaatcgtt ttctttctat
    20341 tgtatccaag gaatttccca aggtcaatcc tttttactaa aattttacta aaatatcagt
    20401 aaaaagaaga aaaatatgat atactaacct aatcaaggag gatttatggc taccttaaaa
    20461 gacattgcac agctagcctc tgtctcaatc gcgaccgtat cccgtgtcct caaccgcgac
    20521 cagagcctat ctgttacaga agaaaccaga caccgtattt taaccgttgc tgaagagcta
    20581 ggctacacca aacatctcaa gacaggcgag tcccacaagc ccaagcaaaa gattgccatt
    20641 atccaatggg tcagcgaaca aggggagcta gacgacctct actactacca gattcgccta
    20701 ggaatagaaa aaagagccca agagttggac tacgatatct tgcgctattt taatgaccat
    20761 ccttttacct taagcgagga agtgattggg attctctgca tcggaaaatt tagccgagct
    20821 cagatttctg cctttgaaga ataccaaaag cctcttgtat ttctagacag cgatacactt
    20881 tccctgggac atacctgtat tatcacggat ttttacactg ctatgaaaca ggttgtcgat
    20941 tatttcctca gtcaagggat ggaccgcatc gggattctaa cagggcttga ggaaacaaca
    21001 gaccaagaag aaatcattca ggacaagcgt ctagaaaact tcaaaaacta cagtcaagcg
    21061 aggggaatct atcatgatga actggtcttt caaggaagat ttactgccca gtctggctat
    21121 gacttaatga aggaggccat tcagagcttg ggagaccaac ttccgccagc atttttcgca
    21181 gccagcgata gtttagctat cggtgccctc cgtgccctcc aagaagctgg aatcagcctg
    21241 ccagatcgcg tcagcctcat ttcattcaac gacaccagtc tgaccaaaca ggtctatcct
    21301 cccctctcta gtattacagt ttatactgaa gaaatggggc gagcaggtat ggatattctt
    21361 aacaaggaag tcctccacgg tcgcaaaatt cctagcctga ccatgctggg aaccagactg
    21421 acattaagag aaagtaccct aaatcaagaa taggataaca taaaaaacga atagagttct
    21481 aaaactccta ttcgtttttt attcgattac aatcatagac ttaattgtct tacgttcatc
    21541 catatctttg taggcttggt cgatatcttc cagtttataa cttgaagtaa agacgcgacc
    21601 tggattgata tcaccatcaa ggacggcttt tagtaaaaat tgcttatcgt atgttgtagc
    21661 agaagctgcc ccacctgcta cagagatatt ttgcataaat gtcgaaccaa gagcacgatt
    21721 attatagtgt gggactccta caaagcccat acgccctcca ttatgaagaa cacctagcgc
    21781 ctgttctata gcagcctccg taccaacaca ttcaagtgct gcgtctgctc ctccaccgag
    21841 gatttcacgc accttggtaa ttccttcttg accacgttct gcaacaacag ctgtcgcacc
    21901 tgactccata gccatctttt gacggtcttc atgacggctc ataaggataa tttgtgatgc
    21961 tccacgcatc ttagccgcga tgacagcaca ttgaccaaca gccccatcac cgataacaac
    22021 aaccttgtcc cctttttgaa catttgcaac acgcgccgca tgatagcctg tcggcatgac
    22081 atctgcaaga gtcaaaaggg actggagcat cccttctgta tagtcagaag gttgaccagg
    22141 gattttaacc agcgcccagt ttgcatagtg gaagcgaata tattctgcct gaaaatcacc
    22201 ccccaaatta ttgccaatat gattgtcgca agaaccgtca aatccagcaa gacaggcatc
    22261 acactcacca catccatgtg taaaagggac aatcacaaaa tcacctggtt tcaccgtcgt
    22321 aatggcttcc acagcttctt caacaatccc aatcgcttcg tgtccactat ttttgtgtcc
    22381 agctttcgtt tctggattac ggtacctcca taaatctgaa ccacaaacgc acgcacgaac
    22441 cacacgaata atcacatcat ccgcttctat tatttgcgga cgttcaatgc tagcaagtcc
    22501 aacctgacct gcctttgtat atactgctga tttcatttaa aattttcctt ccttataaag
    22561 tttaattttg agatttaaac gatttaaagc tgtctgtagc tgagacaagc gctcctctaa
    22621 tttttgcttt tcctcttcta aaataccaag cctctcctct ctcgtttcat ctcccttttg
    22681 gtagagcgac atatagtcaa ctaaactatc tacagagaca cccgccgaac gaaaacactt
    22741 aataaattcc agcgcttcga tatcctgatc ttgaaaatca cgaatcccag tagcagtacg
    22801 agtaatcggt ggcacaagac caacccgttc ataataccga atcgtatccg ctgaaattcc
    22861 caacaaatca ctggcagatt taatattcac acgctaacct cctctcattt cattcactga
    22921 attcacgcta ttctaactca cttatcttat tatataactt tgagttgact ccaagtcaag
    22981 cattttgtat ctaatcgaaa aatttttatc attaacaatc atatatctgg agtttataca
    23041 atgctcttct cttaccaatt tttgaaatat ttaaataata aagaaattac tctattccaa
    23101 ataagatagt acccaagtat ttttcacttt tttctaatgt tgatgctcat aactccgttc
    23161 caagtcacac acctttcgct tatgggtttg gtgagtttct aggtcagcat caatttcaat
    23221 ggtaatattt tgaaaaccac aatcttttag gaaaattcga atagactctt tacaagtttc
    23281 catatgttcc atttctttta gacaaacatg gacaatggca tttttttcca aagcatccat
    23341 agtccagaga ttaagctgat taaggctggc cacattgtcc aatcgctcca ggccactctt
    23401 tacttgcttg atatcaagac cttctggcac agcatccaag aaaatcttga gtgtagacca
    23461 aaaacgtgga agggcttttg aaagaataaa gaaagaaatg acaagggaca aaagaggatc
    23521 taggatatac cagtccgtaa atcgaagaac aatcgccatc aggataacag ctacccaccc
    23581 tagcgtatct tccagaaaat gcagactcag aatagactca ttctttgtct ttcccttacc
    23641 aaccaccaga ctcgctaaca gattgatagt aatcgcaata attcctaacc agagaatccc
    23701 ctcatcattg actggttgcg gatgcaaaat cttcgtgaca ttttccaaaa tgactagaac
    23761 agagcccgtt acgagaatca cagctgttac caaggctcct agcaggctaa accgcttata
    23821 gcccaaggtg tactgattgt cttcttcacg attggagatt gtttctagaa aagctgatat
    23881 tccaattgca atcgcatctc ccaagtcatg cacagagtca gcaagaacag cgctagaacc
    23941 aaatactcca cctgcaataa actcaacaat ggcataagtc aaatttaaga aaaaagccac
    24001 ccaaacagca tattttgcct tcatatttct cattcctttg ttataataga tttatgaaca
    24061 ccttgttcat tatcattatc cactaaaact caaagaaagg atagaagcaa aacgtcagct
    24121 ttattcagtt ctgaacaatt tgccttaagt gtccatatga ctaacattga ccgccgtatc
    24181 agcaaaacca aaaaagccat ctatcaagct tttatacaac ttttgaatgc taagggctac
    24241 gaggccacta ctgttcagga tatcattgat ctcgcagatg tgggacgatc caccttttac
    24301 tgtcactatg agagtaagga gcttcttctg gaccagctct gccgctacct cttccatcat
    24361 ctttttgaaa gagagcaagc catttcaacc gaggactacc tcgcccacct ctttctccat
    24421 tttcataaaa accaagacca tatcaccagt ctgctatttt ccaaaaatga ctacttcctc
    24481 cgtcaactcc ataaagagct agaacaccat gtctattccg tgctagctga taatttaaaa
    24541 gaagcccacc ctaatctgcc tacttcttac ctccaacact tggtcatgtc caactttatc
    24601 gagacattga cctggtggct caaaaaaggt caagatttca cagaccagga agttgtccaa
    24661 ttttatctag accttctcat tcctaaaaat tgaatataga gtaaagcttc agttgtctta
    24721 tttctaggtt actgagtttt ttatcttttc aacaacaaaa gaggacccgc cgatcctctt
    24781 tttcatacta taaatccttg attatcaact atatctgttt taatcgaaat ctcaaaacag
    24841 cactttcaaa catcttttcc tagttaagta aatcagtatt ctgcttagct gccttgctcc
    24901 attgatacca accaactaga ctgttaatga gataaattag atatttccct tgaatttgca
    24961 ggctttctcc ccaccagaga tagattgaaa agacattggt agccgcccag aatatccact
    25021 gttcacggta aacagctgtc atgaggattt gccctacccc attggttgca tctgtgattg
    25081 aatcacgata gggacgattg gcaccaatag actgataaat gaagccaaag gccaaccacc
    25141 aaagcacact aatggaaaga tactttgtcc agcccttgcc gtccagttta cgcgcgacaa
    25201 actcctgctt ttccttctta aactgtgcct gataaatcca aactagaagt ccaattggct
    25261 gcatgactgt gaagtaaagt gtcgtcagca cctcaccata aaagcctttc tgtagggcca
    25321 aaataaggta aataacagag ttaatcaagc caaaaagata attacttgct cgaccttccg
    25381 atacaaagat tacacagata atccctgtca agctacaaat catcccaatc cagtcaacaa
    25441 tacgatgttc gtaaaccaac tccagccaga gaggaaaact tcctaaaacc agcaaataaa
    25501 tccactgggc aaaactacga tgggcaaaga ggtcatccca gatagccttc atagttcctg
    25561 aaaatcctaa atcagccata gccgcaacca tacgacggta accacctgac atttcaccta
    25621 gggttgtttt gatattttca attttctttt gcaaataagt atgcatcatt tctccttttg
    25681 tttttaaaga gccgtgtctg gatagacttt cggacgcaac gctctattag ataatgaact
    25741 gcctatacac aagatttcta accttagtcg acatgagctg aaacctctta tttgttaagt
    25801 agttcacaaa atattataca cctattttat gaatagtcaa ctgtctttac agtaaaattt
    25861 tagaaaatca tgaaaatttt ctctttcttt ccattttaag tgacattcag tcattctcac
    25921 atcaaaaaag cccagacgaa attgtctgag cattctttta tctagtcgtt taaggaagtt
    25981 gagttcagta tgtttaaagt ctctgtccca tcatttcttc aacaaacctt gttcttggag
    26041 aaactccttg gctacttgct ttgctgactt gccttcaaca ccgacttggt agttgagctg
    26101 gctcatctgg ctttctgtaa tcttaccagc caatgtatta agaactcttt ccaactctgg
    26161 gtgtttcttg agaagagctt ctttcatgag tggagcccct tgataaggtg ggaagagttg
    26221 cttgtcatct tccaagacct gtaaatcata acgctccaat tccgcatcag tcgaataggc
    26281 atccgtgatt tgaatatccc ctgactgaat agcctgatag cgaagggctg gctccatggt
    26341 cgctacattg agattgagac catacattga ttgcaagccc ttatttccat cttcacggtc
    26401 gttaaactcg agtgtaaaac ctgccttcaa ctgcccttcc acttttttca agtctgaaat
    26461 ggtcttcaag ccatattctt gagcaatctt tttcggaaca gctacagcat aggtgttttg
    26521 ataagacatg ggtttgagat aggctagatg atcctgctta gcaatgccat cacgcgccac
    26581 ctgataaacc tgttctggtt catgactcac cttgggtgat ggttgaagca aactttcagt
    26641 caccgtacca gtaaattcag gatagatgtc aatatcgcct tttttcagag cttcataaag
    26701 gaagcttgtc ttcccaaaat tcggtttaac agtcgcagtc atgctggtat tttcttcaat
    26761 cagcaactta tacatattgg ccaaaatttc tggttctgga cctattttcc cagcaataac
    26821 caagttttcc ttctcttttt gaaccaaaag agctggacta taagacagac ccagtaataa
    26881 agccaccaag gcaaaacctg agaaaatcgt ccgtaatttt gctttttcca tcacttttag
    26941 taggaagtta aaggcaatgg ctagcactgc agaagaaagt gccccaatca aaatcaaact
    27001 ggcattatta cggtcaattc ccaaaagaat aaaggaacct agtccccctg caccaatcaa
    27061 ggccgccaag gttgccgtac cgataatcaa aacagctgcc gtccgaatcc cagacatgat
    27121 aacaggcatg gcgagtggaa tttcaaattt cttgagacgt tcccatctgg tcatcccaaa
    27181 ggcaatccca gcctcttgca ggttcggatc aattcccttc agcccagtga tagtattttg
    27241 caaaataggg aaaatcgcat aaatcactag agctgtcaaa gccggcaagg tcccaattcc
    27301 catcaaaggg ataaagagcc ccaacaaggc cagagacggg atggtctgga aaatacctgc
    27361 aatctgcaag acccagtcgg ccagcttctc atgatagcga agaaaaacag ccaagggaat
    27421 cgcaagcaaa atagctagta acaaggtcaa aagcgacaac tgcaaatgtt gagatagagc
    27481 tgtcaaccaa tcactaaaac gatcctgaaa agttgcaatt aaattagtca tgaacactac
    27541 ctccaaacaa gtctgctaca aagtctgttg caggcgcttt taaaattgtc tcgggattcg
    27601 ctacctggcg aatttctcca tcctgcaaga cagcaatacg gtccgccaac ttcaaggctt
    27661 catccgtatc atgggttaca aaaatcgttg tcatcccaaa ctctttatgc aattcttttg
    27721 tcagaacctg caactgtttt ctcgaaatag catccaaggc cgaaaagggt tcatccatga
    27781 ggaaaatctt gggctgacca atcatagctc ggacaatacc gacccgttgc tgttctccac
    27841 cagataattc actaggtaag cgatgcccat actcggctac tggtaaacca accttagcca
    27901 aaagctcttc tgttttcttc gtaatttctt ccttgctcca ccccttcatt tcaggaatga
    27961 gagcaatatt ttccgcaact gttagatttg gaaaaagagc aatagcctgt aaaacataac
    28021 cagtagaaag acgaagttca cgctcatcat agtctttgat gcgcttccca tccatataaa
    28081 tatttccatc agttggttcc aaaagacggt taatcatctt gagcatggtc gtcttacctg
    28141 acccagaagg ccctactaaa accataaatt ccccatcctc aatctgtaag ttgacatctc
    28201 tcaagacatc cttttctgtg tagcgcagtg ctacattttt gtattcaatc attctttgtc
    28261 ctcaatttaa aacttccctc gattggtcaa gtcttctacc ttaggcataa cttccttatt
    28321 atcccaatgc tccacaattt tcccgttctc taaacggaag atatcgtact gggcataagc
    28381 aacgccatca atctgagtct gaccatagct aaccacatag tttccttgtc ctaagagttg
    28441 gaaaacaaag tcaaaagtga cactatattc agccacatag tttttataag cagcacttcc
    28501 ttgtccaata tcatgattat gctgaatcaa atcgtctgcc acataatcac tccactgctc
    28561 tagctcccca ttttggaaaa tttctgtcaa gaaacggcga accagctttt tattttctgc
    28621 tttcttgtcc aaatccttga tttcaaaatc tccaaaaatt tgatctagtt ggtcattttc
    28681 aggtgttcga tagtagtcaa cgacatccca atgctcaaca atacgaccat tctcatccgc
    28741 acggaaagta tccgtcgtca cccattgagc ttctccacca ttcagatatt gatgaacatg
    28801 aacaaagacc agattgccat cctcaatggt gcggacaatc ttaatctgac gctctggatg
    28861 acgctcaaag aaatctgcaa agaaggctgc aaatccttct ttcccgtcag gaacacctgt
    28921 cgaatgttgg atataggtat cccctacaga ctgggcttga gcctcagcaa ctcgtccgtc
    28981 ttgaatggca tggatgtata ggttgtgagc atttttcact tgttgtgaca tattctaaac
    29041 ctcatttccc ttctctttca gattcgccaa aattctttct tgaaaacctt caaattggtg
    29101 aatttcttcc tctgaaaatc ctttgtaaaa gatagtatcc aatttctgac tgacacgatg
    29161 ccccacttct ttctgggact tgcctaactc cgttaaaact aaatacttct tacgcttgtc
    29221 ttttccacac ggactaacaa ttacaagctt ttgttcctct agctttttta tcatagtcgt
    29281 cagcgtatta ttcgcaagcc cagtcgcaag cgcgatatct gtcgcagttg cgcagccagt
    29341 ttcactattc cataaaaccg ctaaaatctt gccctgttca cccctataaa gagcctcagg
    29401 atcttgactc agtaactttt gaaaaatccg cccattcaac aaacgaatat gatgggctag
    29461 caaatgacta tctctcataa cacctccaat ttatttcgat atcgaaatga ataaaacaat
    29521 tgtaacactc atcgttctat ctgtcaacta tttcgatttg gaaataattt ttgataatta
    29581 tccacaccac catactccgg ctcaactaac ttttaacgag agtttctaaa ttccttcgtc
    29641 ctccagtcta caaaagcctt ccatccgtac tatcctatat tttatgaggg gacacatttt
    29701 tcctatcaga gcatttattt taaagataga agtaaatcat aattgcttcc atctgttctt
    29761 ttatagtata ttgaagttga actagggcac tgtatcttct aaaacattga taaaaagcga
    29821 tttgaatttc ccaatcaatt tgttcgtatt tatagcattt tgcaactgga ataggacacc
    29881 atgactgcta aaagatttct ataaattcat ttaatttcct caatcaattt gttcatatct
    29941 tatttcatcc cgttataatt tcaccttacc ctatcttttt cgtagcaccc ttcaaacagc
    30001 ctatccccta ccgtttgacg attcctcact tcgctccact tccattacag aagtttcttc
    30061 actactatgg tctcggctga cttctcatga ttccttgtta ctactatttg aacgctcacg
    30121 agataggtct tataaaaaat gctttgatcc acaatggaat caaagcattt taaagagttc
    30181 ctcatacata agcgcagaag tcgcagttcc tctgtacttg gattcttctc ttttgataaa
    30241 gctagccaag ttgagcaact caggtgctgg atgtttggga tttaggagca attcacgatt
    30301 gaccaggcct gagagacgaa ctgcctgcaa ttgctcattt gtagtaggca gttttttagt
    30361 agtctctagg agagcagcaa ctaaatcttc actcaaatca tgtcgagcat gattgtaaag
    30421 atcttttata aggctttcta ggtttggttc taccatccct accacctccc ttatggttta
    30481 ataatgttta atcaaatcaa ccgttgaacg atccaatttc ttcaccaagg cttgtaagaa
    30541 agcttgcgct tctaggaagt catccattgc atagagggtt tggtgagaat ggatataacg
    30601 agcgcagaca ccgatagttg ttgatgggac accaccattt ttcagatgag ctgcacctgc
    30661 atctgttccg cctttaccac agtagtattg gtacttgata ccagcttctt cagccgttgt
    30721 caaaaggaaa tccttcatcc ctggaagaag caagtgacct ggatcataga aacgaatcaa
    30781 ggttccatct ccaatcttgc cttgaccacc gtagacatca cctgctggtg agcaatcaac
    30841 tgcgaggaag acttctgggt caaacttggt tgtagaggta tgagcgccac gcagaccaac
    30901 ttcttcttgg acgttagaac ccagatagag ttcattgccg agtttttgac ctgataaagc
    30961 ttcagctagc tcgcttacca tgaggacacc gtagcggtta tcccaagctt ttgagatgat
    31021 atttttttca ttggctgtca aaattgcaga actatctggt acaatggtat caccaggacg
    31081 gatgccaaaa ctttctgcct cagccttgtc cgcaaaacca ccatcaaaaa cgatatcggc
    31141 aatggctggc atggttggtc ccccctttcc acgagtcaaa tgcggaggaa cagaacctga
    31201 aatcacagga atttcatgac catcacgagt caagagtttg aaacgttggc tgctaactac
    31261 catggggttc cagccaccga tttctacgac acggaaggta ccatctggct tgatttcgct
    31321 gaccataaaa ccaacttcgt ccatatgaga agcgaccaag acgcgcggtg catccacagc
    31381 ttctgaatgt ttgataccaa aaataccacc caagccatct gtcaccactt catccacatg
    31441 cggtgtcaac ttttcacgaa gataagcacg gacaggcgct tcatgacctg agactgcagc
    31501 aagttctgtt acttctttaa tttttgaaaa taatgttgtc atttcagttc cttctttctt
    31561 tcatccattt taccactttt tataggagaa ggatagtggg aaggtggatt tctaagttag
    31621 tatcttagtc ctgctctatc ttaaaaaagg atagtattct cttgcatgta gtgcaaaatc
    31681 tagtaaacat tccaaaatta actcgaatat ttatttccaa acaaaaaaca atacaccatc
    31741 aaagttgttt agatttttca tgaaatttac agaaaatagt tgacttccct ttcttctttc
    31801 tttaaatata tagttggttg agtttggaat agtacgctgt agctgctaaa acatttctag
    31861 aaattaattt gactttccta atagagttat tcatatctta tttcaatcta ctatagtaca
    31921 aaactagaaa aggaaaaaat catgaccaaa acaattcttg ttacaggtga gactggctac
    31981 attggctccc ataccgttaa agctctttta aatgctggct atcaggtgca tgtcctcgat
    32041 aatctctcta caggaaatcg agcggctgtg gatagtcgtg ctagctttaa agaactggat
    32101 gtttatgatg ctagcgcctt aaagtcttac ttagaagaaa atcagattga tgctgccctc
    32161 cattgtgcag gtgaaactgt tgtgagcgaa agtattgaaa atccaagtaa atacttcact
    32221 gccaacgttg ctggtatgaa ccaagttctc aaagtcttat ctgaagttgg cattcaaaaa
    32281 atcatgttct cttcgactgc ttctctctat ggaaataact gtattgacaa gccggtgact
    32341 gaagataccc tgctcgaccc tgtcaaccct tatgcagaga caaaactgat gggcgaacga
    32401 atgatttact ggatggccaa tcgctacgac tggaaatatg ttattttccg ttactttaat
    32461 gctgctgggg ctgaaatgga tgcttcaaac ggtctgcgtg tgaaaaatcc aactcacatc
    32521 attccaaata tcaacaagac cgcattggga caaaatgata gcttaaaaat atttggagat
    32581 gactacgata cacgtgacgg ttcatgtatt cgagattaca tttatgtctt ggaccttgca
    32641 taggctcatg ttaaaggaat gaactaccta tttcaagaag acagttcttc tcaaatcttt
    32701 aacttagaaa ctaaaaaagg ctatactatc aaagaaatct ttaaaactgc tgaagaatta
    32761 ctaaattaaa aaatcccaca cgagattgtt gctcgacgtg ctagtgaccc agccagtgtc
    32821 ctagcagatg cttcaaaagc taaacaatat cttgattgga cggctagcta ctccctcgaa
    32881 gatattattt tatcagatta aggtaaaaac attttagaat aggataacaa aggagaaagt
    32941 cttgttgctc tctccttgtt gtatttatta aattgtcaga aattttattg acagattaga
    33001 aaaataatgt tacatttata tccgcagata tctttcgata ccaaatctac atgaaaggac
    33061 ggggtatgaa actttctcat tatttaattg gcttacttct actcctagtc tttctctcta
    33121 ttagcattgg gaccagtgat ttttcatggg gaaagctatt tgatttcgac cagcagacct
    33181 ggctcctctt tcaagagtcc cgtctcccaa gaactatcag tattctcctg actgcctcta
    33241 gtatgagtat ggcaggcctt ctcatgcaga ctattaccca aaatcagttt gctgcaccga
    33301 gtacagttgg aacgactgaa gccgccaaac tgggaatggt gctgagcctt tttgtctttc
    33361 catcggctag tctgacccaa aagatgctct tcgcttttgt ttcatccatc gtattcaccc
    33421 tcttcttcct agcctttatg accattttta ctgtaaagga aaggtggatg ttgcctctga
    33481 ttgggatcat ctatagcggg attatcggct cagtcacaga agttatcgcc tatcgtttca
    33541 atctggttca gagtatgact gcctggaccc agggctcctt ctccatgatt cagacccatc
    33601 agtatgagtg gctcttctta ggcctcatca tcctgataac cgtttggaaa ttatcccaaa
    33661 ccttcaccat catgaatctg gggaaagaaa ccagcgagag tttggggatt tcctactccc
    33721 tacttgaaaa actggccctc tttctggtgg cgctaacgac aagcgtcacc atgattaccg
    33781 tgggtggctt accatttctc ggagttatcg ttcccaatct tgttcgcaag cgctatggag
    33841 ataatctaag tcaaaccaaa ctcatggtcg cactggttgg tgccaatcta gttctggctt
    33901 gcgatatcct atcccgagtt ctgattaggc cctatgagtt gtctgtcagt ctcttgctag
    33961 gaatcatcgg tagtctcgtc tttatcctac ttctctggag agggggacga aaagatgcag
    34021 actaaaagca aacataccaa gctcttctgg attctcatta ttcttgccat cggagcttgt
    34081 cttctctact tttggcccat cactcacttg tcagcctttg cttggaagtt gcgttcccaa
    34141 aagatcatcg tttatctctt ggtagccatc gcgactggga tttcgaccat tagttttcaa
    34201 accctgacgg aaaatcgctt cctgacgcct agtattttgg gaattgaatc cttctacgtc
    34261 ctactacaaa ccctactact ggtttttgaa agcaagtttc ttcaacttgg caaatcccct
    34321 atcttagaat tcctagtctt acttcttgtc cagtccttct tctttctcgc cttacaaggt
    34381 tacttgaaga cactgatgaa gcaagacctg gtcttcatcc tgctgatctg tctagcgctc
    34441 agaagtctct ttcgaaatat tagcaccttc cttcaagtcc taatggatcc aaacgaatac
    34501 gataaactgc aaaatagtct ttttgcctcc tttcaacatc tcaacacttc catcctagcc
    34561 atcggttctc tgatcatcct cgctttgaca atctttttct ttcgaaaagc agtcgttcta
    34621 gatgtcttgc acctgcaaag agaaacggct cagatattgg gactcgatgt tgaaaaagaa
    34681 cagaaagagc tcctctgggg aatcgtgctt ttgacctcaa cggccactgc cttggtagga
    34741 cctatggcct tcttcggctt tatgctggct aacctcacct acctgattgt caaagactat
    34801 cagcacaagt tactctttat agtggccatt ctggttggat ttattagctt aaccttgggg
    34861 caagccttga ttgaacgagt ctttgcactg gaaattcgta tcagtatgat cattgagagt
    34921 gtgggtggct tcttattctt tatcttacta tataggaggt ctcgtcagtg aaactggaaa
    34981 acattgacaa atccattcaa aaacaggata ttttgcaagg catttcgctt gaagtcagtc
    35041 ctcaaaaact gacagccttt attggtccaa atggtgctgg aaaatcgact ctcctctcca
    35101 tcatgagcag actaaccaag aaagatcagg gagttctcag tatcaaagga cgtgaaatcg
    35161 agagctggaa ttcgcaagaa ctggcccaag aactaaccat cctaaaacag aaaatcaatt
    35221 accaagccaa attgactgtt gaagaactgg tcagttttgg acgttttccc tacagccgag
    35281 gtcgacttag atcagaagac tgggaaaaaa tccgagaaac tctgaactat ttggaactga
    35341 ccaacttaaa agaccgctac atcaatagcc tgtcaggggg gcaactccag cgcgtcttta
    35401 tcgctatggt actggcccag gatacggact ttatcttgct ggacgaacca ctcaacaatc
    35461 tcgatatcaa gcaaagcgtc agcatgatgc agattcttcg acgactggtg gaggaactcg
    35521 gcaagaccat tatcatcgtc ctccacgata tcaacatggc cagtcagtat gcagatgaaa
    35581 ttgtcgcctt caaggacggc caggtcttta gcaagggaag aaccgatcaa atcatgcagg
    35641 ctgacctgct cagtcaactt tatgagattc ccatcacgct agctgatatc aatgacaaaa
    35701 agatctgtat ctatagctag taacataaaa gctcaagtta gagaaccttc agtctcttag
    35761 tcaataagat taagagactc cctaaatcgt tatcacattt taaaaaggag aaattatgaa
    35821 aacatccctt aaactttatt tcactgccct agtggccagc ttcttgctcc tacttggtgc
    35881 atgtagtaca aactcaagca ctagtcagac agagaccagt agctctgctc caacagaggt
    35941 aaccattaaa agttcactgg acgaggtcaa actttccaaa gttcctgaaa agattgtgac
    36001 ctttgacctc ggcgctgcgg atactattcg cgctttagga tttgaaaaaa atatcatcgg
    36061 aatgcctaca aaaactattc cgacttatct aaaagaccta gtgggaactg tcaaaaatgt
    36121 tggttctatg aaagaacctg atttagaagc tatcgccgcc cttgagcctg atttgattat
    36181 cgcttcacca cgtacacaaa aattcgtaga caaattcaaa gaaatcgccc caaccgttct
    36241 cttccaagca agcaaggacg actactggac ttctatcaag gctaatatcg aatccttagc
    36301 aagtgccttc ggcgaaacta gtatacaaaa agccaaggaa gaattggcca agctagacaa
    36361 gagcatccaa gaagtcgcta ctaaaaatga aagctctgac aaaaaagccc ttgcgatcct
    36421 ccttaatgaa ggaaaaatgg cagcctttgg tgccaaatct cgtttctctt tcttgtacca
    36481 aaccttgaaa ttcaaaccaa ctgatacaaa atttgaagac tcacgccacg gacaagaagt
    36541 cagctttgaa agtgtcaaag aaatcaaccc tgacatcctc tttgtcatca accgtaccct
    36601 tgccatcggt ggggacaact ctagcaacga cggtgtccta gaaaatgccc ttatcgctga
    36661 aacacctgct gctaaaaatg gtaagattat ccaactaaca ccagacctct ggtatctaag
    36721 cggaggcgga cttgaatcaa caaaactcat gattgaagac atacaaaaag ctttgaaata
    36781 agccacttca aactcaattt aatactcaat gaaaatcaaa gagcaaacta ggaaactagc
    36841 cgcaggttgc tcaaaacact gttttgaggt tgcagatgaa agctgatgcg gtttgaagag
    36901 attttcgaag agtataaaat tttgacattc taaaacgcat cctatcaagt tcactcaaac
    36961 ctgataggat gcgtttttat cattttgaaa tctatttacc ccacctcatt ccccttcttg
    37021 atttcgttta agggaaaagt ggtctggaac gggatcctta cctgttttcg accagggatg
    37081 acaacgtaaa atccgagcca agcccatcaa taccccctta aaaccatgtt tttcaatagc
    37141 ctgaatcatg tagttggaac aagtcagctc aaagcgacaa gagggtggaa agactggtga
    37201 gataaaacgt tggtaaaagc gcacaggcgc tattaaaatt cgtttcatta ttttttagtt
    37261 acagccatgg tgtgtagttg gctgatttct tttttattaa gacgacggga ttctcctgga
    37321 cggagtcctg tcaagtctag gtgtccgaaa cgagtccgag acaacttatc tacttggaga
    37381 ccaacagctt caaacatctt tttaacctga tggttacgcc cttcatggat ggtcaactgc
    37441 accacagagc gatttttgac tgggtccact ttgagaattt cataaacagc tggcttggtt
    37501 ttcttaccat caatctcaag accacgggtc aaggggcgga gattgtcctt attggccaca
    37561 cctttaacac gcgcgacata aaccttgtca atctcattac gagggtgaat catctcgtct
    37621 gtaaagtccc catcattggt caaaatcaag acacctgatg tatcccagtc caaacgaccc
    37681 acagggtaaa tacgctcttt gacattgggc aagaggtcga caaccgtctt gcgaccctta
    37741 tcatctgtca cactggaaat cacaccgcgt ggtttgttaa gcagatagta gaccttttct
    37801 tcgttgtaga taggttgacc ttcaacttcg accttgtcgc ctgacttgat agtggttgct
    37861 agttcacgca ccacttggcc gttaaccgtc accaagcctt gcttaatcag ctcttctgct
    37921 tttctcctac tggccacacc tgcgtgggca atatacttat tgattctcat cttcttctat
    37981 cctttcacca aataattggc tttcttgggc ttgaatctca agctcatcaa tcactggtaa
    38041 ttcttctaaa tggtttatcc ccatgtaatc taggaaataa tccgtagtca catagaggtt
    38101 ggggcgcccc aatacttcct ttttcccgtc ttcctttatc aggtcaaaag cctgcaactt
    38161 tgccaaggct ccactcgagt taactccacg gatggcatca atttctatcc gcgtaatcgg
    38221 ctgtttgtag gcaataatgg acaaggtctc aagggcagcc cgagacaagc tctggttgat
    38281 aggcgcctta gagtattcct tcaaaatctc tgcaaattga ggcttggtca ccaatctata
    38341 agcaccactt gtctcaatca aagccaaact ggaatctggg tccttttcat acttctgggc
    38401 taattttcct aaactttgct ggatgcctgt cggtggcaga gagaggagtt cagctaactg
    38461 gcggacccga atcccatctt cacccgctac aaacaagagc gcttctattt ttgctaaagt
    38521 actcatcttt cctctctatc aagtctagct ttgaggcact tgactttctt ccttcttttc
    38581 catgagatag atatctccga aactctcctc ttgcacgagg atcagctcct gggttttgat
    38641 taactctagg gttgccaaaa agagggtgat gacctcttgg acattctggg cttccttgaa
    38701 caaatcctgc aagcgcaatt gatctcgtcc aatcaaggac tctttcacga taatcatcat
    38761 gtcctcaatc ttatactcat cccgcaagat agtcgtgtga ttttgtgcaa actcctcttt
    38821 tttcttggct aggatatttg aaaaagccaa aaagaggtca atggtcgtct tgtcatgcac
    38881 aagctccgca tcttcgtaaa tcaactctgt cggcgctttg gaataatact gggcccgttc
    38941 ttggtgcttg gcttccaagt gctcacccaa gagcttgaac ttgcgatatt cttcgatttg
    39001 agagaggagg tcctgctcca ggtcatcccc caagtctgtc acttctgcta ccttcggaag
    39061 gagtttacga ctcttaatca gcatgagctg actagccatg accatgtact cacccgtcac
    39121 ttccagacgc atggcctgca gggttgagac ataggctaga tactgttcga tgacttccgt
    39181 aatgggcaca tcgtagatat ccatctggta cttagaaacc agatgcaaga gtaagtccag
    39241 gggtccttca aaatctttta atttaatatc cattatctat atttttctaa ggtcaggact
    39301 gtttttaatc ctaatttttt tgcaatttcg tacaaatcga ccttgttttc tatttgtctt
    39361 agaataaact gttcacgtaa gacttgagct gataaggatg gaaaaccttt ctccttgaca
    39421 aaagattcta actgacgaaa ggcccactga cgagaatagg gtttctctcc tctttcaaaa
    39481 agataggtct gccccatcaa gggttccaat tctgaaagca aggccgtggg aatggtgaca
    39541 atcctctgtt gggaagcctt gctgattcgc agcacctgaa aatccagatt gatgtccgca
    39601 accttgatgg ctaaaatctc actgggcaag agccccattt ctaggattaa gagcgctagc
    39661 aagcggccct ctggatggtc gctttcctgc caaaaagagt ctaggtatag aatctctggc
    39721 ttttccgtct tcttttcagc ttgtttggct aattccaagc ggtaaaagct gtccacctct
    39781 cctttttgat agagaaagta gagaaattgg ttacaggccg aaatctttcg cttctgggcg
    39841 ctgattttta gattggctag ctgggcttgg taaatcttga gactggtctc agaaatccgc
    39901 tcacctacca tgtctaaaaa ttgctccaaa tcatacttat aggactgctt ggaattgaca
    39961 gacaagccct gcttttcctc taaaaaggct gaaatcctgt ctctcattgg catcgaatct
    40021 catattcctt actaaagtca tgcaagaggg cattaacggc cttgaggatg gacttgcgcg
    40081 taataatccc ttggaaaata ccctctgcat ccacaaccgg taagaaggac tcatctacta
    40141 gcttgtgcaa gacctccgta atggtgaaat caggcgaaac aaccgctacg tccgtttttg
    40201 tcatatgaac gatatccgta tccgccatga tttcttggct caagtcatgc tccatctgat
    40261 aagccataat atctctgagt ccaatcgtcc caacaaactg tttttcatct gtcacaacgg
    40321 gaacacgggt ataggtcatc tgactgagca agagggtcgc atgatccgca ttgtgggtat
    40381 caatcaacac agctagattt ttagcagggg tcaaaaaagt ttcctcctgc cccaacaaga
    40441 aagtctcaaa ctccttggca atcatcgact aaactccttg gacaaacctg gatacacctc
    40501 gtgatctcgt gttaaaaagt ccactttgaa gtaactatca tcaatctcca cacgagcata
    40561 gagacattct ctgatggtac ctcgtggttg gctgatagaa cctggattta gaaagaggat
    40621 cttgccttcc atccaagcac ttggcacatg caagtgacca tagaggcaga tagcggcctc
    40681 ttcctcctga gcccagtagt ccaacttttg aaagttgaaa ttgatgtcaa acaagtgacc
    40741 atgagtttgg ataatcttgg tcgaaccaag ctcagtcacc agacgttctg ggtagccggc
    40801 gtagaagtcc atgttccctt taacaacgcg gatgccctcc caaagtggag aatccggacg
    40861 tagttcagaa tcgccgttat gaaaaacggc atcgactttg cccacatagc gatcacggac
    40921 ttcttccaca atcaagctat cgccatggga atcgctcatt acaatgatgg tttgctttgc
    40981 catgatggaa atacctccaa aagtttctta acggctaagg cacggtgaga ttgactattt
    41041 ttttcttcca gggttaattc agctgatgac tcacctgttt ctcctacaag gaagagggga
    41101 tcatagccaa agccattttc acccttaggt tcaaagttaa tataacctga ccagtctgct
    41161 tcaacaacta aactttcctt atttgggctg gctacgacta gggttgtgtg gaactgagcc
    41221 gagcggtcct tgagttcaaa gaccatggcc aattcgtgca agagtttggc attattttca
    41281 cggtcagttg ctcccacacc tgcgaaacga gctgaccaga cgcctggtaa gccaccaagg
    41341 acatcgactt tgagaccaga atcatctgcc aaaaccatct tgcccgttaa ttgagaaatg
    41401 gtttctgcct taaggcgggc attttcttca aaggtcatac ctgtttctgc tacttcaggc
    41461 aggtcagggt agtcattaag attttccaca tcgtagccta acttatcaaa gatagctcgg
    41521 aattccttgg ttttaccttc gttacgagtc gcaatcaaca aggtttctct gaccttgctt
    41581 gtctcaaaga aatcatgaac attgacccct tctttaggca attctacata caagagttgc
    41641 ccattttcaa ccaagagcag ggccccatga cgttgaataa cttccaagtt tcgtcccatt
    41701 tcttgattaa gcatgtctac cacaaaggag aacaaacggt aggtagaacc atagcgtaaa
    41761 acagtaactg aaagcgggaa accatactct tcatctccaa atattttgga gaattcaaac
    41821 agaggaaaat ctgtcttata gtcgctcaaa ctgttaacgc caccaaaaat actataagac
    41881 ccaacatacc agtcctggtc atccttatat tcataaattt tatttgtcat aattctacat
    41941 gctccacatg aatctctttt tccagccatt cttcaccaat ttgtgcaaaa ctttggctac
    42001 tggctgttgt gtaaaaacgg tgatggagtg gtccagcatc gcgaccacga ttgatttcaa
    42061 aataattgag taagactgag atatcccgta cgcactctgc cccactatcg atgagctgaa
    42121 cctttggccc catcacattt tggataatag ggcgaaggag tggataatga gtacagccca
    42181 aaatcaggct atccaccttt ccaaccaagg gacgcagggt ttcatagacc accttcttgg
    42241 taacactggt tgacagggca cctgactcaa ccaagggagc aaacttggga caggccaagc
    42301 tctccacctg taagtcggga tccagatcat ggattttctg acggtatatg tctgattgta
    42361 ccgtcatggg cgttccaatc actccgattt tcccaccttg actggacttg atggctgccg
    42421 aagctcctgg caaaattaca cccaagacag gaatatctag ttgagccttg atttcttccc
    42481 agacgaccgc agtcgcagtg ttacaagcaa tgacaatcat tttgacatcc ttagtcaaga
    42541 gaaagttgac cagctgccaa gtatattcac gaatttgctc agcaggacgg gggccatagg
    42601 gcgcccgcgc cgaatctcca atatagacga tttcttcatg gggaagctgg cgcatgagct
    42661 cgcgcacaac ggtcaagccc ccgacacccg aatccaaaaa accaattggt cgattatcca
    42721 tacagtcttc tttctagtct tttttctgat tgatcgttca ttatgattcc ttgtactgaa
    42781 cgaactgacc gacagcttga tagaatgtca actacctctc tcttaatcat aatcaaatat
    42841 caatagaatg caaggaaaaa gtctcatccc taaaccataa ccaacatagt gagaagtctg
    42901 gaactttcat cccagacttt tccttattta ttttttcttt ttattgttag caagggctgc
    42961 cttttgttgg cggatgattt ggtggtaaac ttgttgtacc ttagcttcgc ttggtttttg
    43021 accatttgca ctcaaaagag tacgaactgc ttcagcattc aaacgtgggt tgtcagcaaa
    43081 ttctttttcg atttgcttac gaaccaagta cattcctcca agagctcctc ctagaaaagc
    43141 tagcacaatc aatacaattg ctaaaagtaa atccatcatt tttctcctgt ttctttttga
    43201 gttcctttca ttataccaca aactagccac cctgaccagt ttgttctaca ctttcaggga
    43261 tggaatagac agcaaaaaga accctgcttt tctgaaaaag aggtctcctt gctaagttta
    43321 tactcaatga aaatcaaaga gcaaactagg aagctagcca caggttgctc aaagcaccgc
    43381 tttgaggttg cagataaaac tgacacggtt tgaagagatt ttcgaagagt attaatttac
    43441 ataaatagcc agtgtttgat agggtttgag tagaattttc tcagacactt ctgcatcttc
    43501 atagtttgat atcaaaatct gtccattttg gtagactgct ggcaagtcga tttccacttc
    43561 tttagcataa aagttattga gcactagtaa cttttgatcc tcaaactggc gttcaaaagc
    43621 gtagacttgt ttgctatctt caaaggctgg tttgtaactt ccttctgaaa tgattggcat
    43681 ttccttacgc agtcgaatca agtcttgata gaaggtaaaa atcggaccct ggatttcatt
    43741 ttctacattg atgtatttat aggatttacc agctttcaac caaggagtgc ctgttgaaaa
    43801 tcctgcattt tccgaagcat cccactgcat gggaatgcgt gaattatcac gcgacttagc
    43861 ttgaataatc tggaaggctt cttgctgact ctttccttct tctaagagca tctgataggc
    43921 attaagcgat tcgacatcca cataatcagc catagaatca tagtctgggt caatcatccc
    43981 gatttcctca cccatgtaga tataaggtgt cccacgtgac aggtgaatgc tggctgctag
    44041 catggtggct ccttccttgc ggaagttttg aatatcgaca aaacggttca aggcacgtgg
    44101 ttgatcgtga ttgttccaaa agagggcact ccaaccgtct ttatcactca tttccttacc
    44161 ccaactatgg taaagactct tcaactcttc aaaatcaaag ggagccaagg tccacttttg
    44221 tccatcctta tagtccacct tgaggtgatg aaaattaaag gtcatggata attcctgacg
    44281 atcaggcgac gaatagagga cacagttttc catggtggta gaagacattt ccccaactgt
    44341 cataaagcta tcgtcagatc caaaagtggc ttggttcatc atacgcaaat agttatgaac
    44401 gatgggtttg tctgtataag ctggcttccc ttcattttca ggacagtcca ctgaaacctc
    44461 gtccttaccg atcaaattga tcacatcaaa tcggaaacct ttgacaccct tgtcgcgcca
    44521 gaaattaaca accttgaaaa gctccttacg gacattggaa ttgcgccagt taaggtcagc
    44581 ctgggtctca tcaaataggt gaagatagta tttcccagta tccccgaaag gcgtccatgc
    44641 agaaccacca aacttagact gccaatctgt tggttggtct tggatgaaga aaaagtcttg
    44701 ataatactta tcaccagcta gggctttctg aaaccattca tgctctgtcg aacaatgatt
    44761 aagtaccatg tccagcataa agtcaatctt gtgctcttta ccgacacaca ccatttcctc
    44821 aaaatcagcc atatcaccaa aaagaggatc cactgccata taatctgaaa tatcgtaacc
    44881 attatcccgt tgagggcttg gatagaatgg attgagccag accatatcca cacctagttt
    44941 ggctaaatag ggaatttttt cgataatccc acggaaatcc ccaataccgt tttcagtggt
    45001 gtctttgtaa gattttggat agatttgata gactactttt cctttatcaa gtgtcatctg
    45061 tttctccttt tctgataaaa gggaggaagc agtcttccgt ccctatttgt gctatttcaa
    45121 ttatactcaa tgaaaatcaa agaacaaact aggaagctag ccacaggttg ctcaaaacac
    45181 tattttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag agtattagat
    45241 tcgtgtagcg accatgagag atgctccagc ttggatcgtt gtcggataag ttccgggaat
    45301 agtcgctgta taagcatctt ggttggtgat gataacagga gtttctgtca ccagacctgc
    45361 agccttaatg acatccatat caaaacgaat cagttgctga ccaactgtaa cgtgatctcc
    45421 ttggactaca agactttcaa aacctttgcc atcaagacct actgtatcca taccgatgtg
    45481 gatgagcaat tcaactccct cgtcagagac aatgccgatg gcatgcttgg tagggaaaag
    45541 aaccgtcact gtcccattaa ctggagaggt caactcacct tggcttggtt caatgactag
    45601 accttgcccc atgacacctg atgcaaaaat aggatccgtc gcttgactca attctttcac
    45661 ttggccagtt agtgggctga taatttctac cgaagtaagt tctactggtt catggttcac
    45721 aaattctgct tcttcttgag caacgaattc tgcctgcaag ttcgtatcgc cctctgtttt
    45781 tgtaaagaga ccagccttgc ggaagaagaa agtcaagagc attggaacaa caatcgcaac
    45841 tagcatagtt cctgcaaatg gcagcatgta ttgaggttga atagagagaa tacctggcaa
    45901 accaccgata ccaatagaag ccgcagttac attaaaagta acggataaca tgcctgcaag
    45961 ggctgaacca gtcatcccag caacaaatgg ataaatatat tttacgttaa ccccaaaaag
    46021 agctggttct gtaacaccga gataggctga aatggttgca ggaagtgaaa cctgagcctc
    46081 acgctcatca tggcgatgca tgaaataata ggcaaacacg gctgagcctt gagcaatatt
    46141 agaaagagca atcattggcc atagggcagt gccaccagca tccgcaatca attgtgtatc
    46201 aatggcattg gtcatatggt gcagacctgt gatgacaaat ggagcgtaga gggcaccaaa
    46261 aattgcaccg aagagccatt taactggacc agttaaacct gccaagacaa ctgatgaaag
    46321 tccttgtcca attgtccaac cgattggtcc caaaacagta tgagccaaaa tcaaggctgg
    46381 aatcaatgac aaaaaaggta caaaaatcat ggaaatgact tctgggatat gtttgcgcca
    46441 gaaaatttca agataagaca gactcaaacc tgcaagcaag gctgggataa cttggacttg
    46501 gtaaccaata cgattaacag taaaataacc aaaattccaa acccagtttg ccgcgatatc
    46561 agctgctggc gttgaagcaa ccgcataggc attgagcaac tgaggcgata ccaaacagat
    46621 tccgagaaca attcccaaaa tttggctggt tcccatctta cgagaaacag accaagtaat
    46681 ccctactggt aagaactgga agatagcttc accaggcaac cagaggaagt gattaacccc
    46741 tgcccaaaac tgagaggatt ctgtgatggt cttgccatcc aacatcgacc aatggacacc
    46801 ttccaagaca ttacggaaac cgaggatcaa tccaccaaca atcaaggctg gaataatcgg
    46861 agtaaagatt tccgccagag tggtcataac accttgaacc acgttttgat tactcttagc
    46921 tgcagacttg gttgcttctt tggaaacacc ctcaatacct gaaacggctg taaaatcatt
    46981 ataaaagatg ggcacgtcat ttccaatgat tacctgaaat tgacctgcat ttgtaaaagt
    47041 tcctttaaca gctggaattg actcgatagc tttaacatta gccttcttat catctcttaa
    47101 aacaaagcgc atccgtgtcg cacagtgagt tacggcagtc acattttctt tgcctccaat
    47161 tgcctgaagc agatctttgg cttcttgttc aaattttccc atttgattct ccttatactt
    47221 gtcaaagcaa gtttgatttg ataagtatat tgtaaacgat accaaacttg tctgcaactt
    47281 gtttggtata aaaagggtgt ttttttgtaa attttctagc acttgtatgg taaaatagat
    47341 acaggtgttc attaaactag actaaaaacc tattaaagca ggcaaaatga agaaatacca
    47401 acaattattt aagcaaatcc aagaaaccat tcaaaacgag acttacgctg tcggagattt
    47461 ccttcctagc gagcacgacc ttatggagca atatcaagtg agtcgtgata ccgtccgaaa
    47521 ggccctgtct ctcctccaag aggaaggatt gatcaaaaag ataagagggc aaggttctca
    47581 agtcgtcaaa gaagaaaccg tcaatttccc tgtatccaac ctaaccagct accaagaact
    47641 agttaaagaa cttggactgc gctctaaaac caacgtggtc agtctggaca agattattat
    47701 tgataaaaaa tcctcactga taaccggttt cccagagttt cggatggttt ggaaggtggt
    47761 ccgccagcgt gtggtggatg atctggtatc cgttctggat acggactatc tggatatgga
    47821 actaattcca aatctcactc gccaaattgc tgagcagtct atctattctt atatagaaaa
    47881 tggcctcaaa ctccttattg attatgctca gaaggaaatc accatcgacc actcaagcga
    47941 ccgagacaag attctcatgg acattggcaa agacccttat atcgtttcga ttaaatcaaa
    48001 agtctatctc caagacggac gccaatttca gtttaccgaa agtcgccata agttagaaaa
    48061 atttagattt gtagattttg caaaacgcaa gaaataaaag attgagacac cagatctcag
    48121 cctttttctt tatcccaaaa ctaattcatc actgaccaga ctttggccac tatttttctg
    48181 gaagagatgc ataaggtctt caactgtcag atgctttttc tcttctccct tgacatccac
    48241 cactatcttg ccttgataga gcataatgag acgattgccg tactcaatcg catgattcat
    48301 atcatgggta atcatcaaag tcgtccactg atggtgctcc acaatctttt gcgtcaagtc
    48361 catcaccatt tgactggttt tcgggtcaag tgccgcagta tgttcatcca aaagtaaaag
    48421 tttgggtttc accagagctg ccatgactag ggtcaaagcc tgtctttgtc ctcctgagag
    48481 atattgagta tctactttta agcggttttc aagaccaata ttcaactcct tcaaggcctc
    48541 ttggaactgg attctatcct tctccttcac accccaacca agtcctctct tttgcccacg
    48601 tctcaaggca atagccatat tctcctcaat cgtcaaacga gaagctgttc ccatcttagg
    48661 atattgaaaa acacgggcaa tatccttggc tctctttctg acactcagat ttttaatgga
    48721 tttccctgcc agtagaaggt ccccttcgtc caccgataga ttgccagcca agatattcat
    48781 caaagtggat tttcctgctc catttccacc aatcacatag ataaaatctc cctcttcaac
    48841 ctctaaatct aatcctttga ggacatggtt ctcattgacc gtccctgctt caaatgtctt
    48901 gtgaatattt tcaagtgtta acaaacttgc cataactttc tcctcctatt catttctcaa
    48961 tttcagacca cgaatcttta atctcttttg caattctggt gcaaataaaa ccaaagcgag
    49021 caagattgca ttaaagagac gtaccaagtt ttgatctaga tttggaattt catagatatt
    49081 taaaataatc aaacggtaaa caatagcacc gattccgatg gataataagc ggcctccaat
    49141 ggtcaagtcg tgtatcaaga cttccgagct caaaccaaca acaatggttc ctgtcccaga
    49201 agtcacatct gaaaatccat cattttgggc aaacaaggaa ccacataggg caatcaaacc
    49261 gtttgaaatc atgtaaccaa caatcttcat ggtgtctaca ttgactccat tggcctcact
    49321 tatcgggata ttatccccag tcgaacgcaa gaccaaacca atctctgttt tcatcaaaag
    49381 ggtcaagacc aaacaagtaa gagacagact aaactaagtg agaaaacagc tccttcattt
    49441 gtcagaccca agctcgccaa ctgtttaaag acggttgcag aatctcccaa ggaaagattg
    49501 ggtacactgc ccataatttt aatattgatt gaatagagcc ctgtcaaggt cacaatccct
    49561 gtcaagagag ctgggatttt catcttggtg tggagcattc ctgatacaag acctgctacc
    49621 atacctgcca gcaaagcaag taaagtcgca agccaaggat ttgcccctgc ctgtatctgt
    49681 gatacaacga ctgctgcccc cagaggaaag gctccctcag cagttatatc cgcaatatcc
    49741 aaaatacgga aagtcaagta gaccccaatc gccacaatcg accacaacaa accttctgat
    49801 aaactagata acacaaaatt catcttaatc ctccttattc cttgccttct aacttactaa
    49861 tatcaattcc caatgcatct gccatttctt gattggtatg caattccagt ttttcaggca
    49921 actcaactgc tatattttct ggtttctcac cttttaaaac acgaatcagc atgcgagctg
    49981 tctgtcttcc caattcttca taattggttc cgtagttata caagtcacca acagcaatca
    50041 tttctgttga accaccgaat actggaacct tgtgtttaat agaaacctgc ttaactgttt
    50101 ccatggttga cgaaatgata ttatcagttg ggacaaaaac catatccacc tctgccatca
    50161 aactctctgc tgccgccttg acgttattac tgtccaagat tgttttttca acaacggtaa
    50221 agcctttttc tttcagaagt cgcttaactt catcatttta aacaaccgaa ttgggctcgc
    50281 tctgagtata gaggattcca atcgttttag cctttggcaa cactttcttt atcaaattga
    50341 tttgggttga aatggcatct gatgactgat cgcttgtccc agttacattg cccccaggat
    50401 gttctcttga ctcaaccaac ttggcactga ctgggtctgt tacagctgaa aagataactg
    50461 gtgtcgtttg cgtcgtattg gccaagctct gagcagaagg ccttgcgatg gctagaacga
    50521 catcgctgga ttctgctagt tgctgggaaa tcgtttttag atttctttgt tctccctgta
    50581 cattttgcaa atcaatctca atattttccc cctcaacata gccttgtttg gccagctcat
    50641 ccacaaattc ttctctagta gcgtctaagg actggtgggt aataaattgc gaaataccga
    50701 tacgaaagac atctttttct tatctgcctt caactgatgc aaactgatca gagaagtcaa
    50761 gaggagcccc actaccaaga gggggctagt gattttcgaa caactttcat aatgaaactc
    50821 cttctcagaa aagataaaac aaaaaaccgc ctagatacta tctaaacgga tacttgaaat
    50881 aacctaacaa gttacaactt agctagtcca tttagatatt catctatatg gaccacaatc
    50941 aaaaatctta gccacataga tacaaacaaa ttgcttgaac tgtttgcatc catggtgaca
    51001 tgtctacaaa cactatctaa agtagctaaa gtaaaagttt tgattcatag ttacggcttg
    51061 tctcttattc atttcttttt ctgctttctt ttttgatgct tcctatccta ccaccttttt
    51121 catcacctgt caataatatt tcagaatttt ttaaaatttt tcgaaaattc tttcaggaag
    51181 ctttcaaagt ttttcaaatc agtccaacaa aaaaagtttt ataatttcca taaaaattga
    51241 catggaaatt ataaaaccat tattagttta gtcctttttg ataacgtgct aattcggctt
    51301 ggttcgccca aacatagtga cctggacgga tttctaccat agatggctta tcagtctcat
    51361 agtcgtgttg acttgggtcg taaaccttca agaccttctt acgttccaag attggatctg
    51421 ggattggtac cgctgaaagc aaggcttgag tatatgggtg aattggattg ttaaacaatt
    51481 cttctgtttc tgcaacctct acaataacac ccttgtaaat aactgcgata cgatctgaaa
    51541 taaagcgaac aaccgacaag tcatgggcga tgaagagata ggtcaagccg agctcttttt
    51601 ggaatttttt gagcaagttc aagacttggg cacgtacaga aacgtccaag gctgaaattg
    51661 gctcatctgc aataacaaag tctggttgca tgaccaaggc acgggcaata ccgatacgtt
    51721 gacgttgacc gcctgagaat tcatgagggt aacgagtcaa gtgctcagca agaagaccta
    51781 cttcacggat aatactttga actttctctt tacgttcttc ttcatcctta aataaacggt
    51841 gattgtaaag accttcagaa ataatataat caacagtcgc acgttcattc aaacttgcgg
    51901 cagggtcttg gaaaatcatc tggattcgac gaatcaattc cgcagcttgt tcacgcgatt
    51961 tcttaccatt aatcttttga ccatcaaaaa tgatatctcc attacttgta tcatttagac
    52021 cgatgatagc acgaccaata gttgttttcc cactaccgga ctcacctaca agcgagaaag
    52081 tttctccctt gttgataaag aagttagcat ttttaaccgc gacaaacttc ttacttcctt
    52141 caccgaagga aatttctaaa tctttgattt ctactaattt ttcagacatt tccttcctcc
    52201 tagtcagcca gatgggcaaa tcccattttt tcacggatct tatcatggag atttgcaatc
    52261 acagctggtt tttctacttt tggagcatcc tcatgaagaa gccaagtttt agcccaatgt
    52321 gtctctgata ctgagaattg aggagctttt tgttcgaagt caatctgcat tgcgtagtca
    52381 gaacgcaagg caaaagcatc ccctttcagg tcagtataaa gtgacggagg tgttcctggg
    52441 attgagtaaa gatccccttt atcatcagca agctgaggca agctagacaa gagactccat
    52501 gtatatggat ggcaaggggc atagaagact tcctcaaccg ttccatactc aacgatttcc
    52561 cctgcataca taaccgctac cttatccgca atacttgcca ccacaccaag gtcgtgggta
    52621 ataaagattg ttgtgaaatg atactcgttt tgtaaagatt ttagcaaatc aataatctga
    52681 gcttgaatag ttacatccaa ggcagttgtt ggctcatcac agatcaagac atcaggtcgg
    52741 caggcaaggg caatagcaat aacgatacgt tgacgcattc ctccagaata ttggaatggg
    52801 tattcattaa aacgtctatc tgcgtctgga atgccaacct tattcatgta gtcaatggcc
    52861 aattctttcg cttctttagc tgtttttcct tggtgtttta caataacttc tgtaatctga
    52921 ctaccaattg ttttaatggg gtccaaacta gtcattgggt cctggaagat agtcgcaatc
    52981 ttagcaccac gaatttgttc ccaatccttg tgagaagata aagctgtcaa gtcctgacca
    53041 cggtagtcaa tactaccttg ggcaatgcga ccattttctt cgagcatacc tgtgaaggtc
    53101 tttgtcaaaa cagatttacc tgatcctgac tcacctacca aggctaatac ttctccttcg
    53161 actagttcaa gggaaacgcc gcgaatggct gtcaatactt tgtcacgaac gtcaaattcc
    53221 acgacaatat cgcgagcagt caaaattaca tttttttctt ttgtcatttc tactcctatc
    53281 tatgtgtacg tggatcacta gcatccgcta agttttgacc aactacgaaa agggacaagg
    53341 ataccaagac aagggttgtc aatggaatcc agaacaagta agcattggtt gttacgtttt
    53401 gtgaataatc cgaaatcaaa cgacccaaac ttggcactgt aatcggtaat ccaagaccga
    53461 agaaagacaa gaaggcttcg tatgagataa agcttggaag catttgagtc atggttgtca
    53521 caataacaga taccaattga ggcatgatat ttttggcaac aatcttcaag gttggtgttc
    53581 ccaaagtacg tgacgccaag ttgtattcca agtcacgata gcgcaagatt tgcacacgga
    53641 tcatgaaggc aataccaatc catgttgtta cgctcatggc aaaaatcaga ttccagaatc
    53701 cagctccgat tgagtaagtc aagacaataa caatcaaaag aggtgggatg tttgagatga
    53761 cgttgtaaac ttccatcatg acacggtcaa ctgattttga aataccccaa ataccaccga
    53821 caaaaacacc gataaccaag ttaatcactg tcgcaatcac agaaatgagg atggagttac
    53881 gagctccgaa ccagacaccg tcaaagagcg atttaccgtt actgtcagta ccgaaccaat
    53941 gctccgcatt tggcttgata taacgaacac taaagtcgtt taccttgctg acatcattga
    54001 aatcaaactt agaaaacatt gggtagatga aacttatcaa aatgatggct accaagattc
    54061 ccaacatgac tacagttgat tttttcttca taaattgttt aaacactgat ttccagtaag
    54121 aatatgctgg cgcatcaata gtttcagagg caaaatcgtc acgttttaca aactgaaatt
    54181 tttctttatc gattgtagac attatttgcc tcctttctca gtcaatttaa tacgtgggtc
    54241 aataatagtc atccaaatat ctcccaaaag acgtgagaag atagaaatac atgtaaagat
    54301 gaagacaaga ccaacgacca tagagttatt agatgctttt acagagtcaa tcaacatttt
    54361 acccatacct gggaaggcga agactgtttc agtaagggtt gcaccaccga taaccccaat
    54421 aatggcacca ggaattcctg aaaccagcgg aaccatggca tttttaaaga tgtgtttgtt
    54481 tgaaatttct ttttcagaca aaccttttgc acgagcgaaa cgaacaaagt cttgagattg
    54541 caagtcaatc atgtaacgac gaatccaaat ggctgtaaca ggagcaccca acaaaccaag
    54601 gatgactgct ggtaaaacgt aagaacgcca atctccagct cccaagatag ggaatgaatc
    54661 tggaagggca atagatgatc caatcaatcg aacgatgtaa accaaggcaa tcgttggaag
    54721 agcaagcaag aaggtcaaag tccctgttga taggctatca atccaagtgt tcttgaaacg
    54781 agccatggct gaaccaagtg gcacggcaag agcataggca agaaccaaac caatcaaacc
    54841 agtaatagca gagctgacaa tcatagatgg atattggtaa ttactttcag tcgctgtata
    54901 aggatcatct ttcccatagc tagctacttc acgagagtca gcctgactag gtgacttgta
    54961 ggttcttgag taaatattta cagaagacgt tttcttacct gttgggaact gaacttgggc
    55021 agttttggtt tgtccttgac cttgagtaat aacctgaaga actggtgtat tagcataggt
    55081 tgggtaagag tcacctaaat tcaagttcac aaagttttga tgaacaaatg ggaactgact
    55141 gttaaagtac aagagatatt tatgtttagt tcctgaaccg accaatgacc atccgatagc
    55201 tggatcattt tcaaaacgaa ggtagcgttt caagtctgga ttttcagggt cttggatttt
    55261 atttgtatgg tcaatgtcaa tcaagttagc atagaagtga aaaacacgtt caaaaattgg
    55321 aatttcacga gtagcataga attgaccact ttcagtaaat tctcccaaag tccaaccatg
    55381 acctaattga ttgatgtact tttcataaat agctttattg gtcgcatttg cttctactgt
    55441 tacagaagaa tccatgctac ttgccttttc ttgcaactct ttagtatcgt aatactcaat
    55501 gtagcccata cgctcaaaca cagtattttc atagttatca cgtttatcag ccgttgtcgc
    55561 aattttatta tagttaggat cctgcttgaa aatcaatttt cgaggaacca aggtatagat
    55621 aatcgtgtag gtcaaagtcg ttactaagaa aatcgaaacc aatgaccgca aaacacgcat
    55681 aaaaatatat tttttcatat tatttccttt aaaaatccca aaagaacctt ctcctcatgg
    55741 agagaaagtt ctattagaaa ttatttactt cacatgactt gccaattctt tttgagcttt
    55801 ctcatttgat tcagcttttt ctttcaacca tttttcacga gctttttcat actcttcctt
    55861 agtcaccact ttatcttgta atttcaaata tttgaagtaa acatctgacc ccttagagcc
    55921 tgtttgcgca gaagctccag taaatggaac aattcgtgaa agcactggtg ctgcaccaga
    55981 agaagccata gcaggaataa agagtgaact atctgtcaac catgcttgag ccgctgcata
    56041 tttttcataa cggacattca agtcgcttgt ctctctggca gcttcatcaa ctaatttatc
    56101 gtattctttc aaaccaactt gaactactga agggctattt ggattatcaa atcctaaata
    56161 tgtttttgta gtttcactgc tagttgtttt taaaatatcc aggtaagtag atgggtcttg
    56221 atagtctgga ccccatgaaa ctcctcctga tacatcccaa tcctcagatg aagcattggc
    56281 agcatagtaa gtaatattaa ggaattcatc acttgtcatt tgttgaatat caacaacgac
    56341 attttcaaca ccaagaactg tttctacaga ttgtttaaag gactgaatac gagatatgta
    56401 gttttttgat gcttggtcta ctggaacgtc cagatgaata ggaaactgaa cgccgtctgc
    56461 ttctaaagct ttcttagctt tcgcaaactc tgccttggcc ttgtcagcat tgaataaacc
    56521 atcctgccca tcagctaaat tcacaccttt ccactcatca ccataagcag gaagttgagc
    56581 agcgactaaa tcaccaaagg tcttctcacc agctgaaaca aagtctggtt ttacaaataa
    56641 attacgaact gctaaagctg ctccatcttt accattgatt tgagctgagt aagctgagcg
    56701 atcaagagca aaattcaagg cttgacggaa atctttgtta agcaatgcct tcttagtagc
    56761 tactttctct gaatctgtag ttttagaagt atagttgtaa ctttggcgat caatattcac
    56821 acccagacca gcaatcccag agcctgattg tgtgtaatag atattgtcct tgtattcttc
    56881 tgcaacctta gaatagttgg agctggtagg gtaaagacgg gcataactat aagctccact
    56941 agtgaagtta cgctctagcg actcctgatc tgatccatca tagtaagcta gattgatagt
    57001 atctaggtgg acattttctt tatcccaata ttgctcattt tttacaaact ctacagaaga
    57061 ttttgcagtc aaccctttca acaagaatgg accattataa agcaaggatg tcggatctgt
    57121 tggtttagca aaatcgcttc cttttgatgt ttcgaattct tcattcagag gccagaaaat
    57181 agaataggtc aacttagagt tccagaacgg ttcaggctgg ttcaaagtgt attgtaacgt
    57241 ataatcatca accgccttga caccaactgt tgaaaaatct gttgaagttc ctgatagata
    57301 atctgccaag cctttaaccg aattttcagc taaatacata gcttctgatt ttttatctgc
    57361 tgcgtgtttt aaaccgttca cgaaatcttt agccgtcacc tctgcatatt cttctccatc
    57421 agaggtaaac catttaaccc ctttacgaat cttataagtg taggtcaaac catccttaga
    57481 gacttcccaa tcctctgcaa ctgcaggagc aagattaccg taattatcgt tagtgaataa
    57541 accatcaatc ccatttgaag tcactactgt tgtactattt ttacttgaaa tcaggtagtc
    57601 caaggtttct gggtctgctg tataaacata gccataagct ttaggggctg atgaatcaga
    57661 tgattttgaa gaactgcatg ctgcaagtac acctgctgct aataaaacaa gacctgctgt
    57721 agcaaataca cgattttttt tcattttcta ctcctctgtt tatgtgaatt atagattgac
    57781 aaccattata tcacattatc cattaaaaat caaacaaatt ttcagaatat ttaggcttgt
    57841 tggcacaaat ttttcatttt ttttgaatat atgattcaaa ttgtcgttcg aagtgtcaaa
    57901 gactacagtg aaaataggaa atttgacgca gaaactttgg agtttaggaa gacatacagt
    57961 aaaatgaaat acggacggaa caatgtgatt ttggaattca aattaaatta taacaatatt
    58021 gtagaagtat cattctagta ttcaagattc agtttactat gtcttttcac accaacctta
    58081 ttccgaattc aattactttt gtgatttaca tatatagatt aagactatct tttatacttt
    58141 aaaatttctc gctaccttat ccactatatg ctcctcgcta tcacgtttct attcatagcc
    58201 tacgatttca ctattgcttt ctctgacaat tcttatttcc tgcgtcagac ttaaaacgat
    58261 ctatccccag accattttaa tccgctacct cacgacagtc aggcttgggg agcgctattg
    58321 tattcaccgg tagtggatcc ctacagagga cttacacctc agatgcacga catgcccata
    58381 gtataaaaaa atctcctacc caaggtagaa gatttcaaac ttataaaact taatccgtca
    58441 tgtccgatac caacattcga tgctccaatg gaatactgca cataactagc aagaaaataa
    58501 agcctgactg aatccagaag agagccaagt caaaaattcc gtgcacagca accactgtaa
    58561 ggaaagatag ataaaggccg ataatcggac gtttccccga ctcctgactc atatccatca
    58621 tcaagcgaac aggagcaaca gaagacaaaa ctaataaaat ggtacccaca attccgtaac
    58681 tcagaatcgt atcaatataa agactgtggg catgttcatg ataaggagca tgtatccgag
    58741 gataagagtg catataggtc aatggccctt caccccaaaa aggattttgc ttaaacaagg
    58801 ccatcccagc atcccagata gaaatgcgtt cttccataga agagtctaaa gtacccattc
    58861 gaactcccaa atcactagaa aagaggaaac tcaaaccaat cgcgaagacc ccaatactaa
    58921 gccaaaaggc cttccagttt ttaatagtcg taaagagata gataattgct ccagcgataa
    58981 tagcaggaaa ggcagttcga ttttgagtaa agttcaaacc aaagagatta acaaagcctg
    59041 caatcacaca gaatactttc aaccaattca acttggtcgt tgtaaacaga tagaaagcaa
    59101 tcataataca gaaacaacaa ataattccat aataattagg attaaagaag gtcacttctg
    59161 cccggttctg atgccacacc tgcatattgg gtgaaagaaa agcatagtta aatttcttca
    59221 caatttggaa atgttctaaa ctggcaaaag cagctgacaa gacactacca aacaagacga
    59281 actgcaaaat caatcgaaag aatttatggg ataaaatcga ctgatagtgc aaaaagaaaa
    59341 tagtaaatag aaacattcct actgaagcca caagacccat ccaattttgt gcaagaatgg
    59401 atataacagt actatagcta agaaaaagaa gcagcatcgg atgctccccc attttctgaa
    59461 gaatactttt catgtctcct gtaaaaatca aactgataat atataaacag agtacaacta
    59521 caaaaagata aaagggtaaa aagatactta ggataattcc caataaaatc agctctttac
    59581 tagacaaccc cttcagcttt tcaataaagc ctattgattt caaaatgaat cctttctctc
    59641 caaatcagct gattcagata atagtaagct atcctatatt gtaccacttt tttagcaatt
    59701 tgaaaacaaa ggaaacgttt tccaaaataa aaaccctatt ttatccacca tatcaaggct
    59761 tcaaaatgat acttcaactc cattctcaat tacccgataa gtctgatttt gcaaatcaat
    59821 ttctactact gctgttacgg acttatcttt attttgacgt ttgattacaa tgctgtgagc
    59881 tgttggtgtc tctatctcag tagtcccttc tagatcaaag gcttctgaac ggttacggaa
    59941 agaaaataga ttgagaaggg ccttcacaac aggtcgttgc acttcttttg ctatttcctc
    60001 gttgctatag taatgacgat taatatttcg accttcttta gtttcttcta ataatttcaa
    60061 gtcattcttg cctgctaata gacccacata gtaaacctga ggaatacctg gggcaaaagc
    60121 ttgaattaga cgagcgagaa aatacttgac atcatcatct ccaagcgctg aatagtaggt
    60181 tgaattgatt tggtagatat ctaagttgtt atactcggca ctagagtact tacgtttgac
    60241 attggctcca accttataga gttcatttga agcatagtca atctcctcat cggtcaggat
    60301 atccttgaca tctactactc caatcccatc atgggtatct agcgtcgtaa attgcttcat
    60361 cgggctcatc tttaaccact tagccaaacg ctctgttctg gaactgtaaa gagtataaag
    60421 tgtcaccatt ggaagagcaa aatcataaac atagtaatca tggtctgcta ttttaaactg
    60481 aatcgaatag tgttcatgaa tctcaggtaa aagctctgtc ccatactcag cagcgatatc
    60541 tcgaactttg tccaataaat cccaaatatc tggttccaca aagaaatcat tagtatccaa
    60601 tttcttcact gcataagcaa aggcatctag acgaatcaaa tcacacccat tacttgccaa
    60661 gtgctgaatg gtcttacgga taaattccat agttacttct ttggtcacat caagatcaat
    60721 ctgctcctca ccaaaggtat tccacaaatg ttccactgaa ccatcttcaa acacaatctc
    60781 ttgctttggt gcacgatcct tacgcttgta aattaaatct acatcagact gtgtcggacg
    60841 gttttctggc caaaacttat cccagtttaa aaagagagct ttaaattcac tggcttcatg
    60901 tttttcttga tagtccttat aatacttgga ttgacgagaa atatgattaa tcataaaatc
    60961 aaacataaga taatatctct cacctaaacg cttaacatcc tcccaatcac caaaagctga
    61021 gtccacttcg tcgtagtcaa ctggcgcaaa tccacgatca cctgttgatg ggaaaaatgg
    61081 taaaaggtga actcctccaa tagcatctcc aaaatgctct tccaaattat catataagtc
    61141 tttaagatta tttccaaggc tatcagaata ggtaatcaac atggttttat tttgaattgg
    61201 catcattact ctcctttttc taattgaagc caagtatcat atgatctggc ttcataaata
    61261 aaattcattt taaatctcta tttatcatca aactcgtact aatatagact gtgataaaca
    61321 aagtactact ttcttgtttt ctacatagaa ttatcaacaa gctgaactct tcctctgtgt
    61381 caaagactat agattccatg agctcttctt atactattcg aaaatctctt caaaccacgt
    61441 cagcttcacc ttaccgtact caagtacagc ctgcggctag cttcctagtt tgctctttga
    61501 ttttcattga gtattacttc actgccccgt tgctcattcc tgaaatgata tggcgttgga
    61561 agaagagata gacaatggtg atactgataa tgccgaccac gtaagaggca aagcttggtc
    61621 cgtagtcgtt gaaatattgg cctgcgtagt tgtattggaa caaaggcaga gtccacattt
    61681 tggaatcccg gttcaagaca aggagtggca acatgaagtc attccagaac caaagggcat
    61741 tgatgatcat ggttgtcgca tgcatcggtt tcatcattgg gaagatgatg cggaaatagg
    61801 ttgtaaattg attagcccca tcgatctctg ctgcttcatc cagactttct ggaatcgaga
    61861 ttttgatata gccaacatag agaaagaggg tctgtggaat cgcataggtc aagtagagta
    61921 agatcaaacc aaaggtatta gccaaaccga gtttactcat cataaccgta atcggaatca
    61981 tgatgacttg gaaaggtacg aagattccga ggattaagag ggtatacatg atggtaaagg
    62041 cttttctttt actcatattg cgagcgatgg agtaggctgc cataggtata aagatcatta
    62101 ctgcaagtaa agataagaca gtgatgacga cagagttcca ataatagcct ccaatcccat
    62161 cagctaagag acggctaaag ttgtcccatg tgaagttggt tggaaagcca aagaaattat
    62221 ctacaatatc cttagtgggt ttgaaggaac taaagagggt agcaaggagc ggcactaaaa
    62281 tcagaaccga tcctagaatc aatagaatgt atttgccaat cagggctttt ctttcatctt
    62341 gtttcatcat gcttctcctc ttaaatttca aatttcttag atactctcaa ttggatgatc
    62401 gaaatcacta caattaagaa gaacaagatt acggcaatgg cattggcata accgaattgg
    62461 ttgtttttaa aggcatagtt ataaaccaag agcccaagtg aggttgtggc attgtttgga
    62521 ccaccaccgg tcatggcaaa gacttggtca aaggcagtca gcccaccttt tagggctagg
    62581 ataaagacta tagagacact tggtagcaag taaggcaatt caatgttcca gaaaacttgc
    62641 ttgctagtcg caccatcaat ccttgctgcc tctgtaatct cagttggaat agattgcaaa
    62701 ccagctagga agatgatgat gggcatagcc accccttgcc aaagaaggac aaagacagcc
    62761 gcaaagattg ctccccactt agtccctaaa agactggttt ggagaaattc aatatgaagg
    62821 gcattcccaa tcgctggaag accgtagttg aagacttgct tgaagatcaa agccactgtc
    62881 aaaccagata aaacagctgg gaagaagaac caagcacgga agaaggtttg gcctttgatt
    62941 ttagagttca agacacgcgc aatgaagatc ccgagtgcaa tctcaccaac caccatgaca
    63001 atcgtaatga ttgcggtaaa gccaatcgca ttcatgaatt ttggatccat gaagaggagc
    63061 ttaaagttgt ttaagccgac aaatttgtag ttgtaagtca atcctgtcca gttggtaaaa
    63121 ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa ttgcttgtaa caagaggggg
    63181 atgaccacaa aagcccatgc ccaatatttt tgtaatactt ttttcatagt ctctctactc
    63241 ctaatccaca tccgctttca tcgggttaaa gaaggcattc aaatcattga ccatgccttg
    63301 tttatcaccg gtcaagacat agttcatggt caaggtatgg aagtctgctt cactggtcca
    63361 gtattgttgc aaccagacca agtgacgatc cgtaaaggca tattcggtca taccagcaag
    63421 cggtgaatct tctcctgctt gtttgacccc ttcgatcgct gttggagatc cgtccacatc
    63481 gtagtatttt tgcatgactt ctggacgggt catatattcc acaaaggcat tggcttcttt
    63541 tggatgtttg gtggtggctg agatagacca tgccaagtct cccgcaccaa cggttaagct
    63601 ttgtcctttt tcttttcctg gaatcatgaa ggtcccaatc ttaaagttcg gtttttgttc
    63661 attaatcgct gtgatcgccc aagacccatt tggtgtcatg aggacatccc cacgtgcgaa
    63721 ggctccgata acatcggtat agccagcacc ttcccagttc ttttgcttag agccattgat
    63781 gcgaaggatg tccatgacct tgatatcatc tttcataatc ggatccgaca atttaatggc
    63841 atttggttga gaataacgaa ggtattgatt tgcttctttt cctccacctg ttgctgtcgc
    63901 aaaggctaat tgattgtaac cattgagtgt ccaagcatct gcacctgcaa ttccaaatgg
    63961 tgtttgtcct ttagcaacga tatctttgac taactgttca aattcatccc aggtttcagg
    64021 aaccttcaag cccagttctt cgaatttatc tttgttgtag taaattccat aagcattagc
    64081 tgtaaaagga acgttgtaaa ctttttcgtt tacagcatat ttttcagcgt agccattttt
    64141 cacgcgtttc aggtagtctt tgttgctcaa atcttcaaag acacctgctt ttgcccattc
    64201 ttgcagttcg atggactgtg ggtaaatatt gaccacatca ggcacatctc ctgcgagaac
    64261 gcgtgtcttc aatacttcac cagcatttgg tacattgacg actttgacct tgatcttagg
    64321 gttttccttc tcaaaatcac gagtgatttt gagaaggttt tggtcatttc ttttttctgg
    64381 ttgaaatact cgatggtcac tgtgccatcc gcagatttac catagttgga gcaagcgccg
    64441 agcccaacca aagctaaacc tgtagttgca agaagtccga tttttttata ccattccatt
    64501 agaaagcctc ctttataaat ttatacaccc ttatcatagt cttctaaaaa tatactgtct
    64561 actcaaaaaa tctccttggg ataagataac agttaggccc gcatacatta gttctgcacc
    64621 tgagtaaact tcgccatttt cctgtaattt atatagtccc tcttcatcca aatcttttaa
    64681 ttttaaagtt gtttccatgg tctctacaac agataaaacg cgaacgtagg ttacaatcgt
    64741 ttgattttcg taattaaatt gtacagctgc ttcattggat acagtatcag gattaattag
    64801 tctatactgc tgtcctaact gaactactgg tcgtaattct ttatacaagt tcacctgatt
    64861 agcaatcgta gctttctctt catctgataa atttgtcaaa tcaagttcat agcccaaatt
    64921 tcccatcatt gctacaaggc cacgtgtttc taatggtgtc attcgtccca tctgatgatt
    64981 cggtactgct gacacatgag cccccataga aatggttgga tagagatagg atgaaccgta
    65041 ttgaattggt aaacgtgcaa tggcatcagt attatcacta gcccaaactt gtgggaaata
    65101 gcgcatcata ccaagatcat ttcgtccacc accaccagag caggactcaa agagaatatg
    65161 gctgtgcttc tctgtcagat aagaaacgag ttcgtaaagc cccagcatgt actgatgaga
    65221 ttgcatctgt gtctctagat aagttaatcc attccctagc ttagtgatat tgcggttcat
    65281 atcccattta atgtaatcaa tatcatgata agataggagt tgatctaaga cacttttcaa
    65341 gtattctact acctgaggat tggcaagatt aagtactaat tgattccgag aataagtatg
    65401 ctcatagtca ggaacctgaa tagcccagtc aggatgtcga cgatacaaat cactatctac
    65461 agaaatcatt tcgggttcta accaaagtcc aaactgcaaa cctctttcat ggatagctga
    65521 aatcagactt tctagacttc cacccagttt ttcctcatta acaacccaat cacctaaagc
    65581 acgattatca tcaaaacgat tgccaaacca accatcatct aatacaaaaa gttcaatgcc
    65641 aactttctta gcttcatctg ctaactctaa cagtttttct atctgaaagt caaagtaagt
    65701 agcttcccag ttattgatta gaattggacg ttctttttta gaaaattcac ttagcataat
    65761 gtgcttcagt acaaaattct gactttcatg actaatacca gttaatccct gatctgaatg
    65821 ggtcactaaa gctaccggtg tttcaaagta ttcctcagga gctaacttcc aagaaaagtt
    65881 ttctggatta atgccaatag ccacccgaac ttcattcaat tgattttttt gaacaaaagc
    65941 ttcaaagttg ccactataca ttagttgaat agcaaacaca ttcccagcat cctctgtgac
    66001 tccttgttcg catagtagaa gagctggtgt ttgagcatga ccagaagcac ctcggtttga
    66061 gctaatcgaa aagattcctt gttctacctg ttgacgtcta acagtctttt cacgagcata
    66121 agcaccctgc agagttacta tttcgtaatc tgcagctgga aaatcagcca taaaagaaaa
    66181 atctttatgg atgacaactt cctgattact attattatct aatttactgt agctagcaat
    66241 agtcgcatca ttattaaaag tagtataata caaagtcaga ctaagttgag ccttagaatc
    66301 ttctaacatt aagacaagag tctctgtatc gtccatgcta tgtggagaag gtaagccctg
    66361 tggaccattc tgacctttta aaatctttgc ttctacaaat cgaaagtctg ttacttcagt
    66421 tacactatgc tgaacctgta tggttggttt cctaaaatct cctaagccat gttgtccaaa
    66481 aatctgtcgc tgagtatcta aactaaaggt tcgattagta gccgttggat ttcctgaaaa
    66541 ggcatggtct cgttcataaa cactattgga acctttatag ttcttaatag tctttcctaa
    66601 atgtttcaaa agtaagtagc catttcgatt ttcaataatc aaacttagat ttttactctc
    66661 aacataaaat agattattct ctatcctaac tcccatttac ttcacctcat cactttattg
    66721 attatatttt atcacctgaa atcgctttcc aaaatagaaa aatgtctcaa gaatatggta
    66781 aaatgttagg taggaggtag cacatgttag ttttttcaga ataccagact ggaacaatcg
    66841 accttgccct aagcttttat ggatatgagg aatgcacacc taattactct tttggtccag
    66901 ccattcgtga tacatacgtt ctacattaca ttactaaagg acaaggaaaa ttttattaca
    66961 agggtaaaat tgttgattta aaagaaggag atttctttct attaaaacca gaggaactaa
    67021 ccttttatca agcagatagt aaagaacctt gggcctacta ctggttagga atcactggag
    67081 ggaaatcccc tgattatttt gctctttccc aaatttctga tcaatcctat ctcatccaat
    67141 ctgaaacttg tcatacccag actactgcaa aactcatctc agacattgtc cgcttcgctc
    67201 agattacaaa atcaagtgaa ttagctcaac tccatatcat gggacaactt catgaactga
    67261 tgtttcatat gggaactatt gctcccaatc agaaaaaaaa gaatatttca tcaacccacc
    67321 aactctatct tgaatgcaaa cgattaattg atagccacta tcctcaatca cttacaattc
    67381 aagatttagc aaaagaacta tccgttcaca gaagctactt atcaagcgta ttcaaagaat
    67441 ttaatacctt atcacccaaa gaatacctac tctacgttcg aatgcaccga gctagacaac
    67501 ttctcgaaaa tacccaagag tccatcaagg taattgcata ctcggtaggt ttttcagatc
    67561 cactccattt ttcgaaagct tataaacaat actttaatca gactccaagt catacaagaa
    67621 aagaatactc tcaataccaa ctagtgagaa aggcaacatt atgaaatcct accaagctgt
    67681 ctaccaaatc ctatctaaag aaaccgacta tatcagcgga gaaaaaatcg cagaaaaact
    67741 atccctaagc cgaacatcaa tttggaaagc catcaagcga ctagaacaag aaggcattga
    67801 aattgatagt atcaaaaata gaggatataa attgatgaat ggtgacctta ttcttccaga
    67861 gattctagaa gaaaatcttc caattaaagt cagctttaaa cccgaaacaa aatcaacaca
    67921 actagatgca aaagaagcaa ttgatttagg ccatgaagca aataccctct atctagcttc
    67981 ctatcaaaca gcaggccgag gccgttttca acgttccttc tactcaccac aaggtggtat
    68041 ttatatgaca ctccatctta aaccaaatct cccctatgac aaattaccat cctacacact
    68101 acttgtagct ggagctgtct acaaagctat taagaaccta actttaatag atgtcgacat
    68161 aaaatgggtc aatgatatct atctaaacaa tcataaaatt ggaggaatcc ttactgaagc
    68221 aatgacctct gtagaaactg gcttagtcac agatatcatt attggagtag gtatcaattt
    68281 cactattaaa gacttccctc aggaattaaa agaaaaagct gccagcttat ttaaagctac
    68341 agctcctata acaaggaatg aattgatcat agaaatctgg cgtgctttct tcgaaacacc
    68401 agcagaagag ctattatacc tatacaaaaa acagtcattc attctaggaa aagaagtcac
    68461 tttcacacta gagcaaaaag actacaaggg acttgctaaa gacatctcag aaaatggaaa
    68521 acttttaatt caatgtgata acggaaaaga aatctggcta aatagtggcg aaatttctct
    68581 caatagttgg aagtaaaata acacaattat aatataaacg atataaaaat aacttcagat
    68641 tagtaattca attaagtttt acggatctga agttttattg tctctaaaaa caaaaaagag
    68701 agttacaaac tctcattaaa acggagaata agggattcga acccttgcgc cagttacccg
    68761 acctaacgat ttagcaaacc gtcctcttca gcctcttgag taattctcca att
//
LOCUS       DBERCA010000006        62392 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.006, whole genome shotgun sequence.
ACCESSION   DBERCA010000006 DBERCA010000000
VERSION     DBERCA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 62392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.006"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(158..1138)
                     /locus_tag="ACL719_000421"
     CDS             complement(158..1138)
                     /locus_tag="ACL719_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGS0698447.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTPPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1454..3469)
                     /gene="recG"
                     /locus_tag="ACL719_000422"
     CDS             complement(1454..3469)
                     /gene="recG"
                     /locus_tag="ACL719_000422"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGS0698448.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLNGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERETDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMIETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEVWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3488..4591)
                     /gene="alr"
                     /locus_tag="ACL719_000423"
     CDS             complement(3488..4591)
                     /gene="alr"
                     /locus_tag="ACL719_000423"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGS0698449.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGTHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4581..4943)
                     /gene="acpS"
                     /locus_tag="ACL719_000424"
     CDS             complement(4581..4943)
                     /gene="acpS"
                     /locus_tag="ACL719_000424"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGS0698450.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            complement(4988..6019)
                     /locus_tag="ACL719_000425"
     CDS             complement(4988..6019)
                     /locus_tag="ACL719_000425"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS0698451.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6021..7052)
                     /locus_tag="ACL719_000426"
     CDS             complement(6021..7052)
                     /locus_tag="ACL719_000426"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS0698452.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7133..9646)
                     /gene="secA"
                     /locus_tag="ACL719_000427"
     CDS             complement(7133..9646)
                     /gene="secA"
                     /locus_tag="ACL719_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGS0698453.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKKRYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9840..10079)
                     /locus_tag="ACL719_000428"
     CDS             complement(9840..10079)
                     /locus_tag="ACL719_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698454.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLVFGILSILMALLSKKVSLFVFGLICCLSL"
     gene            complement(10107..10739)
                     /locus_tag="ACL719_000429"
     CDS             complement(10107..10739)
                     /locus_tag="ACL719_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698455.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10743..11384)
                     /locus_tag="ACL719_000430"
     CDS             complement(10743..11384)
                     /locus_tag="ACL719_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698456.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11377..12168)
                     /locus_tag="ACL719_000431"
     CDS             complement(11377..12168)
                     /locus_tag="ACL719_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698457.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12353..12688)
                     /locus_tag="ACL719_000432"
     CDS             complement(12353..12688)
                     /locus_tag="ACL719_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698458.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVRH"
     gene            complement(12828..13163)
                     /locus_tag="ACL719_000433"
     CDS             complement(12828..13163)
                     /locus_tag="ACL719_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698459.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13254..14564)
                     /gene="der"
                     /locus_tag="ACL719_000434"
     CDS             complement(13254..14564)
                     /gene="der"
                     /locus_tag="ACL719_000434"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGS0698460.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14578..15291)
                     /locus_tag="ACL719_000435"
     CDS             complement(14578..15291)
                     /locus_tag="ACL719_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGS0698461.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15288..16184)
                     /gene="dnaI"
                     /locus_tag="ACL719_000436"
     CDS             complement(15288..16184)
                     /gene="dnaI"
                     /locus_tag="ACL719_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGS0698462.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16185..17354)
                     /locus_tag="ACL719_000437"
     CDS             complement(16185..17354)
                     /locus_tag="ACL719_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS0698463.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17355..17828)
                     /gene="nrdR"
                     /locus_tag="ACL719_000438"
     CDS             complement(17355..17828)
                     /gene="nrdR"
                     /locus_tag="ACL719_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGS0698464.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17968..18333
                     /locus_tag="ACL719_000439"
     CDS             17968..18333
                     /locus_tag="ACL719_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0698465.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18338..19033
                     /locus_tag="ACL719_000440"
     CDS             18338..19033
                     /locus_tag="ACL719_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698466.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19050..19817
                     /locus_tag="ACL719_000441"
     CDS             19050..19817
                     /locus_tag="ACL719_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698467.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20170..21369)
                     /locus_tag="ACL719_000442"
     CDS             complement(20170..21369)
                     /locus_tag="ACL719_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698468.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVEVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21366..22259)
                     /locus_tag="ACL719_000443"
     CDS             complement(21366..22259)
                     /locus_tag="ACL719_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698469.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22382..22573
                     /locus_tag="ACL719_000444"
     CDS             22382..22573
                     /locus_tag="ACL719_000444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698470.1"
                     /translation="MKSKEQTRKLAVGCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            22966..23154
                     /locus_tag="ACL719_000445"
                     /pseudo
     CDS             22966..23154
                     /locus_tag="ACL719_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23569..24186
                     /locus_tag="ACL719_000446"
     CDS             23569..24186
                     /locus_tag="ACL719_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGS0698471.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24506..25393)
                     /gene="scrK"
                     /locus_tag="ACL719_000447"
     CDS             complement(24506..25393)
                     /gene="scrK"
                     /locus_tag="ACL719_000447"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGS0698472.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25542..27479)
                     /locus_tag="ACL719_000448"
     CDS             complement(25542..27479)
                     /locus_tag="ACL719_000448"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGS0698473.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLIGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGNAVIEVKI"
     gene            27855..29309
                     /locus_tag="ACL719_000449"
     CDS             27855..29309
                     /locus_tag="ACL719_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS0698474.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQNLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDCFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29290..30255
                     /locus_tag="ACL719_000450"
     CDS             29290..30255
                     /locus_tag="ACL719_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0698475.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30290..31564)
                     /locus_tag="ACL719_000451"
     CDS             complement(30290..31564)
                     /locus_tag="ACL719_000451"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGS0698476.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31564..32736)
                     /locus_tag="ACL719_000452"
     CDS             complement(31564..32736)
                     /locus_tag="ACL719_000452"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGS0698477.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLNKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(32867..33115)
                     /locus_tag="ACL719_000453"
     CDS             complement(32867..33115)
                     /locus_tag="ACL719_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698478.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(33183..33808)
                     /locus_tag="ACL719_000454"
                     /pseudo
     CDS             complement(33183..33808)
                     /locus_tag="ACL719_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(33870..34319)
                     /locus_tag="ACL719_000455"
     CDS             complement(33870..34319)
                     /locus_tag="ACL719_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS0698479.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(34321..35079)
                     /locus_tag="ACL719_000456"
     CDS             complement(34321..35079)
                     /locus_tag="ACL719_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS0698480.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(35195..37174)
                     /gene="stkP"
                     /locus_tag="ACL719_000457"
     CDS             complement(35195..37174)
                     /gene="stkP"
                     /locus_tag="ACL719_000457"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGS0698481.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(37171..37911)
                     /locus_tag="ACL719_000458"
     CDS             complement(37171..37911)
                     /locus_tag="ACL719_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGS0698482.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(37926..39239)
                     /gene="rsmB"
                     /locus_tag="ACL719_000459"
     CDS             complement(37926..39239)
                     /gene="rsmB"
                     /locus_tag="ACL719_000459"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGS0698483.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFTNGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(39232..40167)
                     /gene="fmt"
                     /locus_tag="ACL719_000460"
     CDS             complement(39232..40167)
                     /gene="fmt"
                     /locus_tag="ACL719_000460"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGS0698484.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(40180..42576)
                     /locus_tag="ACL719_000461"
     CDS             complement(40180..42576)
                     /locus_tag="ACL719_000461"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGS0698485.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQMVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(42642..42956)
                     /gene="rpoZ"
                     /locus_tag="ACL719_000462"
     CDS             complement(42642..42956)
                     /gene="rpoZ"
                     /locus_tag="ACL719_000462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGS0698486.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(42981..43607)
                     /gene="gmk"
                     /locus_tag="ACL719_000463"
     CDS             complement(42981..43607)
                     /gene="gmk"
                     /locus_tag="ACL719_000463"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGS0698487.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(43737..45350)
                     /locus_tag="ACL719_000464"
     CDS             complement(43737..45350)
                     /locus_tag="ACL719_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGS0698488.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(45541..45795)
                     /locus_tag="ACL719_000465"
     CDS             complement(45541..45795)
                     /locus_tag="ACL719_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HGS0698489.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(45799..46053)
                     /locus_tag="ACL719_000466"
     CDS             complement(45799..46053)
                     /locus_tag="ACL719_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGS0698490.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSIQVHVIEE"
     gene            complement(46291..47388)
                     /locus_tag="ACL719_000467"
     CDS             complement(46291..47388)
                     /locus_tag="ACL719_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS0698491.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHTIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETS"
     gene            complement(47398..48138)
                     /locus_tag="ACL719_000468"
     CDS             complement(47398..48138)
                     /locus_tag="ACL719_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0698492.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(48247..48600)
                     /gene="rsfS"
                     /locus_tag="ACL719_000469"
     CDS             complement(48247..48600)
                     /gene="rsfS"
                     /locus_tag="ACL719_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGS0698493.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(48613..49113)
                     /locus_tag="ACL719_000470"
     CDS             complement(48613..49113)
                     /locus_tag="ACL719_000470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS0698494.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(49114..49707)
                     /gene="yqeK"
                     /locus_tag="ACL719_000471"
     CDS             complement(49114..49707)
                     /gene="yqeK"
                     /locus_tag="ACL719_000471"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGS0698495.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(49707..50336)
                     /locus_tag="ACL719_000472"
     CDS             complement(49707..50336)
                     /locus_tag="ACL719_000472"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGS0698496.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(50388..50699)
                     /gene="yhbY"
                     /locus_tag="ACL719_000473"
     CDS             complement(50388..50699)
                     /gene="yhbY"
                     /locus_tag="ACL719_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGS0698497.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(50914..52020)
                     /gene="yqeH"
                     /locus_tag="ACL719_000474"
     CDS             complement(50914..52020)
                     /gene="yqeH"
                     /locus_tag="ACL719_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGS0698498.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(52023..52550)
                     /locus_tag="ACL719_000475"
     CDS             complement(52023..52550)
                     /locus_tag="ACL719_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGS0698499.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(52565..53473)
                     /locus_tag="ACL719_000476"
     CDS             complement(52565..53473)
                     /locus_tag="ACL719_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS0698500.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(53530..54090)
                     /locus_tag="ACL719_000477"
     CDS             complement(53530..54090)
                     /locus_tag="ACL719_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGS0698501.1"
                     /translation="MQKFIQAYIEKLDVTSIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            54229..55447
                     /gene="sstT"
                     /locus_tag="ACL719_000478"
                     /pseudo
     CDS             54229..55447
                     /gene="sstT"
                     /locus_tag="ACL719_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            55515..56468
                     /locus_tag="ACL719_000479"
     CDS             55515..56468
                     /locus_tag="ACL719_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS0698502.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEKIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            56612..56992
                     /locus_tag="ACL719_000480"
     CDS             56612..56992
                     /locus_tag="ACL719_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS0698503.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            57092..57313
                     /locus_tag="ACL719_000481"
     CDS             57092..57313
                     /locus_tag="ACL719_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS0698504.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            57330..57644
                     /gene="trxA"
                     /locus_tag="ACL719_000482"
     CDS             57330..57644
                     /gene="trxA"
                     /locus_tag="ACL719_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS0698505.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            57950..58483
                     /gene="queF"
                     /locus_tag="ACL719_000483"
     CDS             57950..58483
                     /gene="queF"
                     /locus_tag="ACL719_000483"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGS0698506.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKPLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(58693..59361)
                     /locus_tag="ACL719_000484"
     CDS             complement(58693..59361)
                     /locus_tag="ACL719_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS0698507.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(59472..60263)
                     /locus_tag="ACL719_000485"
     CDS             complement(59472..60263)
                     /locus_tag="ACL719_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGS0698508.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWIHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            complement(60688..61452)
                     /locus_tag="ACL719_000486"
     CDS             complement(60688..61452)
                     /locus_tag="ACL719_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698509.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAILMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(61445..62215)
                     /locus_tag="ACL719_000487"
     CDS             complement(61445..62215)
                     /locus_tag="ACL719_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698510.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT        17948 a        14145 c        11890 g        18409 t
ORIGIN      
        1 attgttagaa atcgatttga ctgtcctgac cgatttgtcc tgttcttatt tcattttact
       61 atatttccat gataaaacgc atagtataaa gttttcttaa tcccctgata ctatgcgtgt
      121 ttgtaatttt taagattttg tgcttagagt cgactcctta ttttagatat ttaaaaggaa
      181 tctcacttcc acagagccag ttgtagactt ggtcattgac aaatacattc atggcttcgt
      241 gagcatactc aggcatgatg cgataggttt tatcgcaggt cagacgatta taaatcgcaa
      301 actgggtaat gggatagcaa acatcgtcgt ccaagcccgt aatcatctta acctcacctt
      361 ggatacgatg ggcaagattt ttgacatcga tataggcaag ggtcgccatg atttcctcct
      421 ctgtttcatg gaaggggtcg tgaaacttga aataacggaa aagttcgtcg taagcctcgc
      481 tagtattacc aatctcaatc acccgtctga agtctgacaa gaagggataa atggcaactg
      541 ttttctgaat tcgaggattg agcgctgctg caactagagc tagagcccct ccttgtgagg
      601 caccatagct agaaagacgc ttctcatcaa cctgagacag actagcaaca atttcgacca
      661 actggtaaat atccagataa acatccttat aaaagaggtg gtcccgacct tccatagcac
      721 cacggataat atgccccttc acggtatttc ctaaaggaga acgcaagccg tcttgtgagt
      781 aacctgactg gccccgcaca tccatggaaa caacaccgta accagctacg gtgaagccca
      841 gcatgtcggc ccagtcccag ccacgtccca tataaccatg aaaatggaag attagtggaa
      901 ccttctcctc actctttgga agaacaatgc gtgcatagac ctttccttcc ttgcttcctt
      961 caaatgttaa ctcatagcac ttgacttgag gaatgtggaa atctctttcc tccaagtggt
     1021 aggatggagg tgtggaaaca tttttcactt ccccatccca gaaagcatca aagtcttccg
     1081 gaacctcatc ccttcctcta taggtcttaa tttcttctag caaagctgga tttttcatag
     1141 catgctcctt ttattttgta atcgttacca caactgtagc atatctatgt aatcaatgca
     1201 agaaagaagg ataaatagaa agtgaattta gattctttac ttttaggatg atgggctgaa
     1261 agtagtttta gaaacgaaaa agagcatttc gctctctctt ctgtttatac tagttttttg
     1321 cttccttctt ttggaaagtg gtttggtaat ttactttaat cgttactgtc atatgagagt
     1381 cctcgtttct ttttgatgac tttagtataa gagccaaacc taaaaactag cttaagtttt
     1441 ccttagagaa agcttaatcc agatgttctt tcttttccag atgaagggca atcatgcgcc
     1501 actctggatc ttcttgccaa acttctatag aactaatgta gctagcaacc tttcttgctt
     1561 cttctaaaat cggaaaatct tcgataatat cagccacttg gaactctgga agtcctgact
     1621 gtctggttcc aaaaatctca ccagaaccac gcattttcaa atcttcctcc gcaaggacaa
     1681 atccattggt cgtttctatc atgatgcgca tgcggtcttt cccagaatcc gtcttgggat
     1741 tggcaacgag aacagcgtag gactgcttgt ccccccgacc gacacgacct ctaagctggt
     1801 gaagttgact gagaccgaag cgatcggcat ccatgataat catgacagtc gcattgggaa
     1861 cgttgacccc aacctcaata accgtcgtcg aaaccagaat atccgtctct ctctccttga
     1921 aatcctgcat gatctggtct ttttcgtcac tcttcatcct accatgtaga agagccacct
     1981 ctgccttgcc tgcaaaatga gtcgtcaact cctctgataa ggcaatggca tttttcaaat
     2041 ctagagcttc tgattcttca atcaaaggag agatgacata gacttgggaa cctttttgaa
     2101 tttccccctc taaccaagtc aagacctgag gtagttgctc atgtttgatc cagcgcgtca
     2161 caataggctt ccgacctgct ggcatctggt cgataatgga aacatccata tctccaaagg
     2221 ctgtgatggc aagcgtccgt ggaatgggag tcgccgtcat catgaggaca tctggattgt
     2281 cacctttttc ccgtaaaata cgcctttgcc ctacaccaaa acggtgctgc tcatcgataa
     2341 taatcaaacc aagacgagca tactccaccc catcttgtat cagagcgtga gttcctataa
     2401 tcaaatcagc ctcacccttg gcaatggtct ccaagacctc tctcttttct gcagctttca
     2461 aggaacctgt caagagagcc agtttcaaat tgggaaaaag gttctgtaaa ctctcaaagt
     2521 gttgctctgc gaggatttct gttggtacca ttagggcagc ctgataacct gctgtcactg
     2581 ccgcaaacat ggccaagcca gcgactaccg tttttccact ccccacatcc ccttgtagga
     2641 gacgattcat gtggtggtcg gacttcatat cagttaaaat ttcctgcaaa ctcttttcct
     2701 gagcttgggt cagggcaaaa ggaagactta ctttaactgc tgtcactttt tcctgagacc
     2761 aattcagaac cagaccactt ccctgaactc tattttcaga cttgagcatc tgcagctgca
     2821 tttggaaata aaagagttcc tcaaacttta tacggcgaag agcctgcttg tattctgcca
     2881 aatcctttgg aaaatgcata gctcggactg cctgacaacg ggacatgagt ttgtatttgt
     2941 ctagtaaaga ctggggcaga ttttcttcta tcaagaggtc cagtccctga tcaaaagccg
     3001 tcttgatgac cttaaccaga ctggcctgac tgattccctg agccagacga taaacaggct
     3061 ggaggtcatc ttctacctga gccagaacct tcatcccatt cagactagcc ttagcgcggt
     3121 cccattttcc aaagacagca agggttgctc ccaactctat tttatcagcc agatagggct
     3181 ggttaaagaa attcaccgca aaaacgacct ctccctgctt gagactaaaa cgcaggcgat
     3241 tgcgcttgaa accataatac tggacactag caggagtcac tacctgacca gaaagaactg
     3301 ccttctcacc gtcttccagc tccagcacct gcttggtttt gaagtcttca taacggaaag
     3361 gaaagtagag caagagatct tgcaagtttt caattcctag tttggcgtat ttttctgctg
     3421 actttggtcc cacaccaggc aagacatgca agggttgatg tagattcatg ctccactcct
     3481 ttcttttcta ataatattct ctcggaatac ggtcgctgag gaggcaaacc acctcatagt
     3541 taatggttac gcggtaggtc gctacctgag ttgcagtgat ttccttatcc ccattggagc
     3601 caatcaaggt taccttggtt cctagcggat aaagcttagg caatcgaata gtgatttggt
     3661 ccatcgaaac cctgccgaca attgggcaag cttggccatc taccaagaca gagaaatttt
     3721 gcatgtctct tgtccatcca tctgcatacc cgattggcac ggtcgcgatg acttgctcgc
     3781 tatccgcttg ataagttgct ccatagccca tgcaagctcc tgctggaact gtcttgacat
     3841 gaaccagagc agactccaag gtcaaggccg gtatcaaatc ataaggcaaa tccaagaccg
     3901 ctccacttgg attgaggcca tacatggcat ctcccatacg aaccgcattg aaaatagtct
     3961 ctacatgcca aagagtcgtt gcagaattgc tagcatgaac cagctctgga acttccttca
     4021 tactagctaa aatagtttta aaccgttcta actgggcatt aaaatagtca tctgattcct
     4081 catcagcagt agcaaagtgg gtaaagattc cttcaacacg aacaccgtgt tgttgtagca
     4141 aatcttgagc ctgctcaacc tcactggcct ctctaaaacc aatccgtccc atccctgaat
     4201 caatcttgag gtggactgtc aatccagtta ggtccacttc cttatctaag agtgcttgaa
     4261 tccactccag tccagccact gtcaaggtga agtcatattc tttagctaga gcaacagctt
     4321 cgatttcaga aactcctaaa atgaggattg gcttgctgag tccagcttgt ctgagttcaa
     4381 tggcttcatc gatattggaa acgcaaaagc catcaacatc atcttgaatt gccttggcaa
     4441 cggcaacagc tccatgacca taagcattgg ccttgaccac agcccacttg agcgttcctt
     4501 gagggatatg agtccccatt tgctgaatat tttgtcgaat agctcccaga tgaatcagaa
     4561 ccttggttgg tctatgtgga ctagctttca tgattttcct ccaaaatgac actggctgtc
     4621 acaaactgat cggtgtggct gatagacagc caaatctttc ctgaaaatgg tgcctgacta
     4681 aaataaggcg ccccacgttc attgttcaat acttccaaat cctgaaaacc gagcttgcta
     4741 atgcccgttc ccatagcctt ggaaaaggcc tccttagccg accagcgacc agctaaatat
     4801 tctatttgcc tgcgtccttt gagactggtg aagcgctcca tttcctgagc agtcagtaca
     4861 cgcttagcaa atccttcatg tcgtgtaact gcgctttcta tcgaagccaa ttcttcgatg
     4921 tcaattccgt gtccaactat cattttcatc aaaaggagtt gagattcccc aactccatcc
     4981 ttttcactta ttttgtcaag gtaacataaa tctcttctac caaggcctct gtattttccc
     5041 aacctaggca agggtcagta atagagcaac caaagatctc tggttggttt tgacgaccat
     5101 ctgctaggta agattcaatc ataaatcctc gaaccgtctt tttaattttc tcattccaat
     5161 cacgattctg caaggtctgg cgaacaattc gaatctgctc catatattgc ttgcctgagt
     5221 tatcatggtt ggtgtcaatg aggataaaag gattttcaag tcccatggtt tcatagcgtt
     5281 caatggcttg gagtaggttt tcatagtagt aattcggcat ataattgcca tactcgttga
     5341 ctgctccacg gaggataaca tgggccaaag gattacctga tgtctcaact tcctgcccat
     5401 gataaaggaa ggtttgcttg ttttgagcag catagatggc gttaaacata acacccaaat
     5461 ttcctgaggt tggatttttc atccctactg gtgcatcaat cccagaagcc acaaagcggt
     5521 gctcttggtc ttccacagaa cgagctccaa cggcatggta gctgaccaag tcatccacca
     5581 agatcagatt tgacggataa agcatctcat ctgccgttgt taaaccagtc tctgtaatca
     5641 cgcggtagtg caactggcgc acagcctgca agccgttaat caggcttgga gccttagaag
     5701 tatctggctg gtgaaccaat cctttatagc cgtctccatt ggtacgaggc ttagcagtat
     5761 acacgcgcat gaccatgaaa atcttatccg ctaccttctt ttgcaaggcg gataaacggc
     5821 gagcatattc caagaccgcc tcttcattat cagaagagca aggaccaatc accaagagaa
     5881 tacggtcatc ttcccctgaa ataatatctg ccaattccct gtcacggctt tccttgagtc
     5941 tcaaggcttc cgcagacaat tgggtttcag ccttgatgac ttccatatcg atttcttgac
     6001 ctttttcaat aaatgccatc ttattctcct aacgttttgt aaatttctct gacaagagct
     6061 tctgtgttat cccaacccag gcaagggtct gtgatagact taccaaatac ttctggctca
     6121 ttttgtcgac catcttccag ataagactca atcataaaac cacgaacgaa ctgcttaatt
     6181 ttttcattcc aagcacggtt aatcaaggtc tggcggacaa ttcggatctg ttcaatatac
     6241 tgcttaccag aattgtcatg attggtatca atgatgataa aaggattttc caagcccatt
     6301 ttctcatact gggcaatggt atcaattaaa ttgtcataat agtagttggg aatatttttt
     6361 ccatactcat taagagcacc acgaagaata gcgtgtgaaa gcgggttccc agttgtttct
     6421 acttcttttc ctaagaaaag gaaactttgt ttgttttgag cagcataaat tccattaaac
     6481 atgacattga gatttccaga ggttggattt ttaaaaccag tagaaaatcc tgccccactt
     6541 gccacaaagc ggtgttgctg gtcttcaact gaacgggcac caactgccat gtaagaaatc
     6601 aaatcatcta caagcggaag gttttcagga taaagcattt catcagcagt tgtcatccct
     6661 gtttctgtga tgacacgata gtgaagatgg cgaacggctt tgattccatt gataagacta
     6721 ggcgcttctg tcgcgttagg ctggtgaatc aagcccttat agccatctcc gttggtacgg
     6781 ggtttggcag tataaacacg cataaccata aagatacgat ctgccacttc ttcttgtagg
     6841 actgccaaac gcttagcgta ttcaaggaca gcttcttcgt tgtcagatga gcatggcccg
     6901 attaccaaga gaattcgctg atcttctcca cgtataatgg cttctagctc ttgatctcgc
     6961 tgtgatttcc tctccaaagc ctgaccttct aattttgaca aggcacgaac ttcttctata
     7021 tttattttag agctttttgc tgtaaatacc ataactcatc tccttataaa gcaaacggat
     7081 acccagtaaa aatttacttt tcacaagata tccgcctcta aactatctca ttttattgtc
     7141 ttttaccgtg acagttttta aatttcttac cagaaccaca tgggcaaagt tcattgcgtc
     7201 caatctggct caaatccaaa tcttctggca tacttgcttg gtgagcagcg atattgcgag
     7261 tcgctgttgt actgatatga cgttctgcct gtggtctttc ttgttcatga atttgtgctt
     7321 tcatcatcaa gcgtgtcaca tcaaactcaa tcgaaccaat catatcatta aacatacgga
     7381 aaccttctgc ctgatactca acaacagggt tgttctgagc atagccacga agtccaaccg
     7441 cgttacgcaa ttgatcaagg gcatcgatat gatctgtcca cttgttatcc accactcgta
     7501 gaatcaaaac tttttggaat tctttaactg cttcttcatc gcgtagtttt gaaacctgac
     7561 tatcgtaaac cttcaaggca cgttggaaaa gctcttcctt gatggcctta tcagacaagc
     7621 ctgacaagtc ttccatcgta atagaatctt caggaagcaa gttgtactta gcaaagttca
     7681 aaattgcctc tagtttttca tcttgtttag cacgcgcatg accgtcaacg acacgttcaa
     7741 tcgtgcgttt gatcatagac tgaatttcag gtgccaagtc acgatctgca gtgatgacat
     7801 cgtaacgttg agcatagata atctcacgtt gttcacgcat gacatcatcg tattgaagga
     7861 cttgtttacg ggtatcgtag ttatttcctt cgacacgttt ctgagctgct tcaacctgac
     7921 gcgtcaacat gcgagactca atcgcctctt cagacatgtt gagacgttca aagattccct
     7981 tcaagcgttc agaaccaaaa cgtttcatca aatcatcttc aagagatagg tagaattgtg
     8041 actcacctgg atctccttga cgacctgaac gtccacgaag ctggttatcg atacgacgac
     8101 tttcatgacg ttctgtacca ataacacaaa gtcctccaag ttcacgaaca ccttcaccaa
     8161 gcttgatgtc ggtaccacga cccgccatgt tggttgcgat ggtaacggca ccacgttgac
     8221 cagcattcat gatgatttgg gcttctctat agtggttttt ggcattcaag acttcgtgag
     8281 gaacaccagc tgcgaccaat ttcttagaaa tgtagtcact agtttcaacc gctactgtac
     8341 caaccaagac aggttgaccc ttttggtaac gagccttaac gtcttcgaca accgctttaa
     8401 acttagattc gatacttgca taaagaaggt ctgagtggtc aatacgttga acaggacggt
     8461 ttgttgggat tggaataaca cgaatgttgt agatttcacg gaattcttct tcctcagtct
     8521 tacctgtacc cgtcatacca gacaatttct tgtacatacg gaagaggttt tggtacgtga
     8581 ttgaggcaga tgtcttggtt tcatcctgga ttggcacacc ttctttggct tcaatagctt
     8641 ggtgcaatcc atcagaataa cgacgacctt ccatggtacg acctgtaaat tggtcgacaa
     8701 tcaagatttc ttgctcttcg ctcaccacat agtcaatatc gagaagcatg atgtagttgg
     8761 cacgaagggc gttatcgata aagtgggtca aagccacgtt ttcgatgtca tagaggtttt
     8821 caagtttgaa gtagctttca gccctgtcaa tccctgaatc agacaaacca atagtcttag
     8881 actgcacatc gatgatgtag tcatctttgt tcaaagattt tacatagtgg tctgccatgt
     8941 gatacaactg actggtttca accgcattgg cacctgatac aatcaaaggt gtacgagcct
     9001 cgtcaatcaa gatagagtca acctcatcga ccaaggcata gttaagcgga cgttgtacca
     9061 tgttttcagc gcgaacgacc atgttgtcac gaaggtagtc aaatccgatt tctgagttag
     9121 ttgagtaagt aatatcacac tcataggctt cttttttctc cattggagat ttggtagcca
     9181 agttaatccc tactgacaaa ccaagccaag agtacaattc acccatctca gtcgcgtcac
     9241 gttctgacag gtattcatta accgtaacta cgtgaacccc tttacctgaa agggcattga
     9301 ggtataccgg catggtcgca gtcaaggttt tcccttcccc tgtacgcatc tctggcacgt
     9361 caccatggtg aagaacaatc ccccccatga cctgaacctt ataagggaag agacctagga
     9421 cacgtttggc accttcacgg acaaccgcaa atgcttcgta aagcaatgaa tccagtgatt
     9481 ctccattttg ataacgtttc ttaaattcaa ctgtttttgc ttttagttgg tcgtcagtca
     9541 aagcagccat ttggtcttcg tatttgaaaa ccttgtcagc catcttttcc agacgacgga
     9601 tttctccttt atcattttcg ataattgttt ttaaaatatt agccatgttt ttccttactt
     9661 taaattccga atattttaga atgttccttt aattttagca caattactga ttattttcaa
     9721 ggaagaatcc ccattttgaa aaggaaaaag aggttagttt tcaagctaac ctctccgact
     9781 tttatgactg tttaattgcc aaaaattggc aaaatggcaa atgtgataaa taagttaatt
     9841 caaagagaaa gacagcaaat cagtccaaaa acaaagagac tgactttctt ggacagcaag
     9901 gccatcaaaa tggacagaat tccaaaaact agcaagattt tctgtatgat gaagatgtca
     9961 atccttaccc ccagaatcgc tctatcccaa gggataaaga agaaagacag ccagatcagc
    10021 agaactaaac caatataaac cttggaatag cgtgtaataa atgattttag atgtgccaca
    10081 agccatctcc taggattgta tatcctttac atttgtatat gtccattttc gacaactaat
    10141 acatctgtac aaaggtctga aatatcttcc gatatgtgcg acgtcaggat aaccagacca
    10201 ttttcttttt tcaggtaaga taaaatgacc tgtttggtta acctcacact cttctcatcc
    10261 aaagcattca taggttcatc gagaaagagt atagaaggag aggaaataaa ggcttgaatc
    10321 aaggccattt tttgctttgt tcccaaggat aaatgccggt attcaacgtc ttcaaattcc
    10381 tgtaaatcat agtattgaat ccaataggca attctttttt ccgtaacttt agatgaaaaa
    10441 taccttaaca gttccaaatt ttctctcagg gataaatgag ataaaaactc gactttttca
    10501 attaaaattc ctgcatcctg aatataatga ttttttatcc cgtaaacttt accatcttga
    10561 agaacttttc ctttgtcaag cttaatataa ccagcaagta cctttaaaag aaccgtcttg
    10621 ccagatccat tatcaccttt aagtccataa atttttcctg attgaaacac caaattcaac
    10681 tgatttaaaa taggtctttt cccataaatt ttctgtccat ttataatttc aagtttcatc
    10741 gtttatcctt tcttaaattt ctcttccaag cgactaacaa tcaacataac taataagctt
    10801 aagagtagta aatacactgt tacaatatga tccatcatcc attcttgtcc cacaaatgct
    10861 aaaaaataga gaaaaaagga aaaaccagcg cccgcactaa tcagtgctat cagataagac
    10921 agtagtaagt acaacatcat taactgagca agtaaaaaga aaagtgcgac tgttttgaaa
    10981 tgaattccta gcggaagtaa accaaggaac cagacaagta aatcttgagt aaaaaattta
    11041 gaaatgacaa ccaatcgttt ccttgtataa tcgaaaaagg agcttgattg atagcgaatc
    11101 aagtgatggt aatggtctgc caaatcactt tcaagtaaaa tgacagagag ccatcctacc
    11161 atgaaaagcg aaaggtttaa caagagtttg gggatttcta aattcgcttc agaatagtca
    11221 ttataggaaa agattgaaat caattctctc ttgaaaaaga taacagcatc ctgcttttga
    11281 accgctgttg acaatagata gtttatcaaa atcatcatca gaactatacg aagtagtaaa
    11341 acgatctttc tctttagcca aatattgtta aaattcttaa acattatctt catatttttc
    11401 aagcaccata taagggacaa tataaaggat ataaggagta aagagtttca tcaaacttac
    11461 atccccatag ctagcttgca tcaaactcgt cataggaacc atatagtaag gcaaggcact
    11521 atagagcagc atagaaccaa gccaaagaaa catcaaatag gtgattgccg aacgagtcac
    11581 attccccaca taatcaacta tttgtaaaaa atagattgca ttgatgaaaa taccgattag
    11641 taggacacaa gtaaagaaca aatagctttt tatctctcca tctaggagtc tttgtaaacc
    11701 acttccatca gaaaatagag aattccatcc aagaggagaa aaagtcccaa agaaataggt
    11761 tataattgca attatcagca cagtcactaa atatatcaaa caagggatac ttgcaacttg
    11821 caaagtcaac tttcttttta acccttgata actcacttct cttccaatac tcaatcgtaa
    11881 aactttattt ttcagctcaa tatattgaaa acctagcaaa acaatgatga ttggaataat
    11941 aaatgattga taaaaaccta gattgaactg gagaatacga aaatctaatg ctgattttaa
    12001 taagtcattt ttaattgctt gtccatcgaa tttagctaga tcgggcgcat gaatatcaaa
    12061 aaagaaataa gtcatcaaaa catcacgata aaccaaagca agaaaaagca aagtaagcaa
    12121 gacataaaca aatttatgcc ttgccaaagt taagagagga atcttcataa gccacctcct
    12181 acaaatagaa agtaatataa tactcaatga aaatcaaaga gcaaactagg aaactagccg
    12241 caagttgctc aaagcactgc tttgaggttg tagataagac tgacgaagtc agttacatat
    12301 ataatccaag gcgacgttga cgtggtttga agagattttc gaagagtata aatcaatgcc
    12361 ttacgccttt agacttccct agttcccgtc tcaagtcaag catttttttg aaacagatat
    12421 agtctaacca gtcactagca aaagcgataa gtagtaataa tagccaaata ccccataact
    12481 tgatatctgt caaatttctc aagacgatat tgaaaaacag aactgaaaaa actgtccaag
    12541 caaggctaaa aagagaatag agggtgatgt aaatccagta aaaatagtaa aaaattggga
    12601 aaaatttact ttctctatta tccttgtcaa tccagctgaa aaagtaaaaa cagggtaata
    12661 agattatcat tttaaacaag tgttccatca caagcctcct tttttaaatt gttttcttta
    12721 gtttcgttct attaaatatt attatcaata taaaccttgg aatagcgtgt aataaatgat
    12781 tttagaggta ccataagcca cctcctacaa atagaaaccg atataaatca atgccttcca
    12841 cccttagact tccctagttc ctgtctcaag cgaaacattt ctttgaaaca ggaataagtt
    12901 aaccaattca taccaatagc tagcagaata aaaagaaacc aaatgcccca taacttgata
    12961 tctgtcacat ttctcaagac ggtattgaaa aacagaactg aaacaactgt ccaagcaagg
    13021 ctaaaaagag aatagaaggg gatgtaaaac cagtaaaaat aataaaaaat tggaaaaaac
    13081 ttactatttc tgttggcctt ttcaatccag ttatcaaaat aaaagtacgg tgctaaaagt
    13141 aagaatttaa acaaatgttc catcaccgac atcaccctcc ttcttttgat agcgttttct
    13201 attattttat tatatcaaaa aaatccggaa ctgtcattcc agattctact tttttatttg
    13261 cgttttcttg cgatgagatg aatcggtgtt ccctcaaaaa caaaggcctt gcggatttga
    13321 ttttccaaga aacgcaggta agaaaagtgc atgagttctt cttcattgac aaagatgaca
    13381 aaggttggtg gtttggttgc cacttgggtc gcatagaaaa tcttgagacg ttttcctttg
    13441 tctgtcggtg ttgggttgat ggcaatggca tccatgatga catcgttcaa gacagctgat
    13501 ggaatacgtg tattttgact ttcgctgatt tgcttaatca tctcaggaag tttgtggaga
    13561 cgttgcttgg ttaaagctga tacaaagata atcggtgcgt aaggcaggta ttggaactgc
    13621 tcacggatat cttcttccca gtttttcata gtgtggttat ctttttcaag cgtatcccac
    13681 ttgttgacca cgataatcat ccctttacca gcttcatggg caaatcctgc gatacgcttg
    13741 tcgtactcac gaatgccttc ttccgcattg atgaccatca agaccacatc tgaacggtca
    13801 atagcacgca tggcacgcat aacagagtat ttctcagtat tttcataaac cttaccagac
    13861 ttacgcatac cagccgtatc aatcatggta aactcttgac catctgtatc tgtaaagtgg
    13921 gtatcaatgg catcacgagt tgttccagca acaggactag caataacacg gtcttcgccc
    13981 aagatagcat tgatcaagct tgattttcca acgtttggac gaccaatcag gctaaactta
    14041 atgacatctg gattttcttc ttcatattca tttggaaggt tttccacaat cgcatctagt
    14101 acatcccctg taccgatacc atggacagat gagataggca gtggttcacc caaaccgagg
    14161 gcatagaaat catagatatc atttcgcatc tcagggttgt ccaccttgtt aactgcgagg
    14221 ataactggtt tgtgggtctt ataaagctta cgggctacgt attcgtctgc atcggtaatt
    14281 ccttccttac cggacacgac aaagacgata acatctgctt cttccatggc aatttctgcc
    14341 tggtgcttga tttgttccat gaaaggagca tcgacatcat caattcctcc tgtatcaatc
    14401 atgctaaaag aacgattgag ccactcaccc gttgcataaa tacggtcacg tgtcactcct
    14461 tcgacatctt ctacaatgga gattcgctca ccagcgatcc gattaaatag ggttgatttc
    14521 ccaacattgg gacgtcctac aatggcaata gttggtaggg ccataatttc tcactttcta
    14581 caataatttc ttctgttcaa gattttttct agttgagctt ggttcagctt gaccaaactg
    14641 ttctgctagg cgctgactcc agcttgtggt cgcacgcgcc ccagcatagt cagcctgaac
    14701 acggtcataa gcttggattg cctcagttga ctgttcttgg tattcttcct caaagacaac
    14761 attctctagt ggcagtctcg gtttcatatc atgatgttga tttggcacac ccagtgccat
    14821 cccaaagaca gaataggtgt agtcaggcag gttaaagagc tctgccactt cttcagactt
    14881 gtatcgaacc aaaccgataa tcacaccacc atagcccaag ctttcagctg ccaacaaggc
    14941 gttttgtcca gcaagagctg catcgaccga actaatcaag agaccttcca caccttgggg
    15001 ttggaaggtg tcggtatgaa gtcgggctcc cttttctgct cggttcaaat ctccgacaaa
    15061 gagaaggaaa acagcagact ggcgaatggc ttcttgaggt accaattcat acaaggcatc
    15121 tttcttctct tgacttcgta ccacaatcac agagtaggat tggaaattct tccaagatga
    15181 tgccatctgg gctgctgtca aaatctcatt taagtctact tggggaattt cttgctcttt
    15241 aaacctgcgc actgaagtat gagccttcat caatttaatg gtttctgtca tcgacggttt
    15301 actccttcta aacgagtctc ctcagccaaa taacggatgc gttccatgac ccgtctggct
    15361 tcccaggttt cgtcatttcc atgtttcact ttcgcaaaat gcttctccaa atcttcaaag
    15421 ttgaagttgg atgtgaaaaa ggtcggtaaa ttttcctgca tccgatattg gagaatgacc
    15481 tgcaggattt cgtcacgcac ccaaacggtt gattgctcgg cgccaatatc atctaaaatc
    15541 aggacctcag acagcttaat ctcatccacc aaggtcttaa cattgccatc actgatagca
    15601 tttttgacat caatgacaaa gctaggatag tggaggagag ttgatgaaac accacgtttt
    15661 tctgataaat catgagctaa ggccgccacc atgaaacttt tacccacacc aaagtctcca
    15721 tataagtaaa gaccttttcg aatagctgga tattgctcca cgaaggctag tagcttttca
    15781 aaaactggta agcgccccaa atcatccaag tcaacttgag ccaaactagc tttcttgaga
    15841 ctggctggta gattgattaa cttgagacgg ttcttaatag ccgcttcttt ttcagccgcg
    15901 attagctcag gagtttcttc atatgaaaca tctgcatagc catgattctt aaccaaaatc
    15961 ggcttgtagc ctttggcaat ataatccgta tccccacgga gaaacttgtc acgctcggtg
    16021 atgtactgat taaacttgga gatactgcga tttaattcct ctggagttaa ggattcttgc
    16081 tggataaagg ccgcaacatc agggtccttc atgattttct ggaccaaatc ttgataataa
    16141 aaacggctgg gttgacgttt gagtacgtct ccgacacttt ccatctaatc tcctcctttt
    16201 tctaatcgag ctaatagttc ttgcttctta cgttctagtt ccagacgagt ttcctcgctg
    16261 gtttcattct tatattcagg attactccat ttaggaacat tggttttttc tggggcagtc
    16321 tgattctgtt tttgtgtttt tgctttctgc cctcgatcac gaattcgtaa aacggcctct
    16381 tctgccgaat gaatcttttg ataggcatag tcattggcta ccttcatggc atatttctca
    16441 ttgatatttg ccgaatccac cttattaaag gtcaataaga gaataatatt gatgacttcg
    16501 tccagtaagc ccaagccagc catctgttgc aagagttctc tttctgtttg ggtaatggtt
    16561 cccttgcgtg tttgcttgat ttctgctaag aactgcaggg cagttttact tttagcttct
    16621 ttgataatgg tcgcttcctt aagactaaag tcagaggaaa ctggtttttg agcaattttt
    16681 tcacgcatgc gtttggttga aataacctgg gaaacagctg ttgacttggc caattgatag
    16741 gtttcaaacc aagtccattt cttctcctcg gcaatagcaa agaggtttaa gacatcggac
    16801 tgctcatccg caaaacgaag tccatctcga gccatcagct ggcgaaaatg ttccaagtca
    16861 aaatcattgg ccactttctt cttgagacca aggtcttctt gactgcctag ttctgccaat
    16921 tctggaaaga cttgattgag tgagacaggt atttcttcac catcagcact ttcaactttc
    16981 aaatcctcca cagctacatc gccaatcttt ttctctaaga gtctgcgata aacaggatgc
    17041 cccaagaagt cttgactaga tagaggagca tggagggcta gctgataaac atcacccttt
    17101 tgatagaggg tcaagagatt aaaagcagat aagattttca atgattttat cagtctatcc
    17161 atcccaaagt tgagatggtt gagaatgctt gaaaaaagat attcctttct accattatcc
    17221 caaaaactga ttgtataaag ataaaggctc agtgcctcct gaccgataat cgggaggtag
    17281 cactgtacca gagatgaggt atcttgcgac acccgattat tctttagata agaaaaacgg
    17341 tcaattggct tcatttatct ttcctttttc tttttagagg actgggtgat ttgttggagc
    17401 aagctctcta actcactgac atccttaaaa ctacgataga cactagcaaa acgtacatag
    17461 gtaatctcgt ccaattcagc caactcctcc atgacgagtg aaccaatgtc ctcactttga
    17521 atttcatttt catttcgacc acggagtttc tgttcgatac gattgactac catgttgatt
    17581 tcatcacttg acacaggacg tttctgggct gagcggataa tcccattaaa gattttatct
    17641 ctggagaatt gttcccgtgt gccatctttt ttaacaacca ctaaggttct ttcttctact
    17701 cgttcgtagg ttgtaaaacg gtgttggcat tcgtcgcact cacgtcttct acgaatggtg
    17761 ttcccttctt ctgcttggcg actatcgata acacttgact tggtagcccc acattttgga
    17821 caacgcatcc tttccctcct tatcgttttc ttttcattat accatttttt aagcgattcc
    17881 caaaacaatt cttctttttg cttgacaagt tttttgtttt gttgtattat ttaattaaga
    17941 caacaaggta aaagaaagga gactaagatg tcctggacat ttgacaacaa aaaacccatc
    18001 tatttacaga ttatggagaa aatcaagctt cagattgttt cccatacact ggaacccaat
    18061 caacaacttc caaccgtgag ggagctagct agcgaggctg gtgtcaatcc caataccatc
    18121 caaagagcct tatcagacct tgaacgagaa ggatttgtct acagcaagcg aacaactgga
    18181 cgatttgtga ctaaggataa ggagctaatc gcccagtcac gcaaacaatt atcagaagaa
    18241 gaattggaac acttcgtttc ctccatgacc cattttggct atgaaaaaga agaactacca
    18301 ggcgtagtca gtgattatat taaaggagtt taagcctatg tcattactag catttgaaaa
    18361 tgtatccaaa tcatatggag caacaccagc ccttgaaaat gtttctcttg acattccagc
    18421 tggaaaaatt gtcggccttc ttgggccaaa cggctcagga aaaacaaccc tgattaaact
    18481 aattaatggc ctcttacaac cagatcaagg acgtgtcctc atcaacgaca tggacccaag
    18541 cccagcaacc aaggccgttg tagcttattt gcctgatacg acctatctca atgagcaaat
    18601 gaaggtcaaa gaagccctaa cctacttcaa gaccttctat aaagatttca atcttgaacg
    18661 cgctcatcat ctacttgcag acctgggcat tgatgaaaat agtcgtctca agaaactatc
    18721 aaaaggaaac aaagaaaagg ttcaactgat tttggttatg agccgtgatg ctcgtctcta
    18781 tgttttggat gaacccattg gtggggtgga tccagcagcc cgtgcttata tcctcaatac
    18841 cattatcaac aactactcac caacttctac cgttttgatt tctacccact tgatttctga
    18901 tatcgagcca atcttggatg aaattgtctt cctaaaagac ggaaaagtcg tccgtcaagg
    18961 aaatgtagat gatattcgct acgagtcagg tgaatccatt gaccaactct tccgtcagga
    19021 atttaaggcc taagcaaagg agattattta tgttttggaa tttagttcac tacgaattta
    19081 aaaatgttaa caagtggtat ttagccctct acgcagccgt gctagtcctt tctgccctca
    19141 tcggaataca gacacaaggc tttaaaaatc taccttacca agaaagtcag gctactatgc
    19201 tactttttct agctacagtc tttggtggct tgatgcttac acttgcgatt tcaaccattt
    19261 tcttgattat taaacgcttc aaaggtagtg tctacgaccg acaaggctat ctgactttga
    19321 ccttgccagt ttctgaacac catatcatca cagccaaact aatcggtgcc tttatctggt
    19381 cattgattag caccgctgta ttggctctaa gtgctgttat tattctggct ttaacagctc
    19441 cagaatggat tcctctttct tatgtgatta catttgtaga aacacatctc cctcagatct
    19501 ttcttacagg tatatccttc ctactaaata ccatttcagg aatcctctgc atctacctgg
    19561 ctatttccat tggacagctt ttcaatgaat accgtacagc actcgctgtt gcagcctaca
    19621 ttggtatcca aatcgtcatt ggatttattg aacttttctt caatcttagt tctaatttct
    19681 atgtcaattc actggtagga ctcaatgacc atttctatat gggagcaggt atagccattg
    19741 ttaaagaact catattcata gctatctttt atctcggaac ctactacatc ttgagaaata
    19801 aggttaattt gctttaaata atttttacct agatatgtaa catactcata gaacaaaaga
    19861 gaccaggcaa aaagtcttta aaattagaaa acgcatagta tcaagtgtta aaaatccttg
    19921 atattatgcg ttttttgtga gttgacttat ttcctttcac tatatcgcaa aatgaaataa
    19981 gaacggaacg atgggatttt ggaattcaaa tcaatttgta agaatgtttt agaagtaata
    20041 ttatcctatt ccagattcag ttcactatac aattgagttt tcaagcaact tgtttacata
    20101 atgtgtacat aattaggttc gtgattccac ccttttcacc tttaaaaacc tcgctttcgc
    20161 aaggctcttc tatttataag ataaggcacg tttaaaggtt ttccaaatcc ctaaatcatc
    20221 cgtttgaaga acgagactag catacatgcg tccgataaat cctgttgcta ccaccgcaaa
    20281 aatcactgta atagcaagtg aaatccatgc ttccgctccc cccgcatagc cattaatcgt
    20341 tcgaaacggc ataaagaagg tcgaaataaa gggaatataa gaaccaatct tcaagaggag
    20401 attgtcacca gctgcaccta gagctgtcac tccaaaaaaa ccacccataa tcaaaatcat
    20461 caaaggcgac aaggctttcc ctgagtcctc aggacgagaa accatagatc ctaggaaggc
    20521 tgccaagact acatacatga aaagactgat caaaataaag agcagggtat tcagtgagat
    20581 agcatctccc aagtgatcca aaataccaga ctgagccaag aatggcaaat ctttaaagag
    20641 caaaacggca gccagaccac ctacaacata gatcccaata tgcgttaaaa tcactagaaa
    20701 cagagccatc atccgcgcat agaaatagtg acttgccctt atgctagaaa aaacgacttc
    20761 cataattttg gtgccttttt cactggcaac ttcctgagct gttacacccg cataggtaat
    20821 cagaatcata taaagaaaga atcctaaggc acctgctgca attgtttgaa taaacttttt
    20881 attttccttg gcttcatcaa tcttttctgt gaattgaatt gtctgcgcta agcgtttttc
    20941 ctgctcttga gacaaggaag cagttgaacg attaagctga ttttgcagtt cattgagtgt
    21001 acctgtaacc tcaaatttaa ttccattttc aagcgatgtt tcgccatgat aaactgcctt
    21061 tagaacacta tcttcttgat caatggtcaa ataacctttt aatttttctt ctttaattgc
    21121 ttctttggca cttgcttcgt ctttatagtc gaagttaaca ccatttacat tcttcagtcc
    21181 ttctgctaca gatggcactg ttgtcactac ttccacttta ttatttttag ccatagaaga
    21241 accttggaga tgcccaattc ctacagagat tcctaaaaag aggaacggcg aaatcaccat
    21301 aaagaagaaa ctccatgact cgacatgtcg aagataggtt tccttgatta caacccacat
    21361 atttctcata cttccactcc tgattctagt ttaaagattt catcgatagt tggcgcttgt
    21421 tggtcaaatg ttgcgatata ttgaccttga gtcaagattg ggaagagttc ccttccagcg
    21481 ctctcatcct ccaaaatcaa tttccaactg ccttgtttgg tcaagctcac ctgtttgaca
    21541 tgaggaagat tttccaattc ttccttgctt cgttcacttg aaacaaagag acgcgttttc
    21601 ccgtattgat tgcggacatc ctgaactggt ccgtgcaaga ccacacggcc atctcggatc
    21661 atcagaatat cgtcacaaag ttcctcaaca ttggtcatga catggtcaga aaagataatg
    21721 gttgctccgc gctctttttc ctgaaaaatg acttgtttga gcaattctgt attaactggg
    21781 tccaatccac taaaaggctc atccaagata atcaggtctg gttcatgaat cagagtaata
    21841 atgagctgaa tcttctgctg atttcctttt gacagactct tgattttatc tgtcagcttt
    21901 cctttcactt ccaacctctt catccattga gggagttttt ctttgacttc tttggcatcc
    21961 atgcctttta gagtcgccaa gtagcgaact tgttcaagaa ctgtcaattt aggcatgaga
    22021 ctgcgttctt caggcagata accaatccga gcataggtct cctgacgaat atcctgacca
    22081 tctagaccga tttctccctg atattctagg aatttcaaaa tactatggaa aatcgttgtt
    22141 tttccagcac cattttttcc gactagtccc aaaatacgac ctggtcgcgc ttgaaagtca
    22201 ataccaaaca aaacttgctt ggagccaaaa cttttctcta gacttcttac ttctagcatc
    22261 tttcacctcc gaaatttctt gcactcatta tactcctttt tgatagcctt tacaatgttt
    22321 tttgtccatt tttagaagac tattgctgtg taaaatatgg cctggagcac ttttatactc
    22381 aatgaaaagc aaagagcaaa ctagaaagct agccgtaggc tgctcaaagt acagctttga
    22441 ggttgcagat aaaactgacg aagtcagctc aaaacactgt tttgaggttg tggatagaac
    22501 tgacgaagtc agtaactata tctacggcaa ggcgaagctg acgtggtttg aagagatttt
    22561 cgaagagtat tagtgataaa tccattatac agtagcaaac ttaatttata ccttccgctc
    22621 ctcaactgtc tatttttaat cctgaattgt tatttgagta actccttttt cctcataaag
    22681 ttttcttcct ctaaaacttc tggaaaaaag cgaatagttt cagataacat ttttataagg
    22741 aacaagttca tctgtcattt caagaaggag taatccttta tctactaatg gacggaacgg
    22801 aattcaaccg cttgtccgat atgttttcta aggattatat agtaaaatga aataagaaca
    22861 ggacaaattg atcaggacag tcaaattgat ttctaacaat gttttagaag tagatgtata
    22921 ctattctagt ttcaatctgc tatatctatt atgcacacca ctataggatc taataaaaat
    22981 cacaacaagt tcattcatag atggttacct cagggaacta agaaaacgac tacaaaggaa
    23041 gtcgcattca tcgaaaagtg gattaacaac tatcctaaaa aatgcttgaa ctacaagtcc
    23101 cccagagaag acttctggat gactaacttg aacttgaaat ttagcaataa ttaattcacc
    23161 atctaactat atttagtaat tatttcagaa ctgattaata ttaaaattaa ctaacaattc
    23221 aaaggattca tactagccat aaattacgtc catcagagag agactcttac tacttttaga
    23281 ttttagtctt tctagcttca gaatacatct aaactttagg gaaaatgact attcgaaagc
    23341 gcgaatgcct caaaattatc tcagataagc tattcgaaac ttagaatgct tttaaattta
    23401 tggaattgcg attattcgaa acctagaatg catataacct ttagttgaca gacctattct
    23461 aagtctcgaa gggctattta ctttctattc cttatcaaaa aagactcatt ccccctttct
    23521 cctccaaaat atggtatagt agaaatatac tatctatgag gagtttacat gtcacaggat
    23581 aaacaaatga aagctgtttc tccccttctg cagcgagtta tcaatatctc atcgattgtc
    23641 ggtggggttg ggagtttgat tttctgtatt tgggcttatc aggctgggat tttacaatcc
    23701 aaggaaaccc tctctgcctt tatccagcag gcaggcatct ggggtccacc tctctttatc
    23761 tttttacaga ttttacagac tgtcgtccct atcattccag gggccttgac ctcggtggct
    23821 ggggtcttta tctacgggca catcatcggg actatctaca actatatcgg catcgtgatt
    23881 ggctgtgcca ttatctttta tctagtgcgc ctatacggag ctgcctttgt ccagtctgtc
    23941 gtcagcaagc gcacctacga caagtacatc ggctggctag ataagggcaa tcgttttgac
    24001 cgcttcttta tttttatgat gatttggccc attagcccag ctgactttct ctgtatgctg
    24061 gctgccctga ccaagatgag tttcaagcgc tacatgacca tcatcattct gaccaaaccc
    24121 tttaccctcg tggtttatac ctacggtctg acctatatta ttgacttttt ctggcaaatg
    24181 ctttgacacg taaaaaatcc gtttggtttc ccaagtggat ttttaaagcg tagattaact
    24241 atagcttgat actaaatata ctttggtatg gaaatcatgc atatttttcg atagtgaggc
    24301 gaggacttac ctagcctttc cgccgtgata gaaacacctg aaatctaatg gtttcaggta
    24361 ttcggaaact ttgagacact aggctcaaag tttaggtatg gaatttcgaa gaaagtcgct
    24421 accgtccgta atcacttaag gaaaggctca aaaatattgt tttcaaccac aaaatccgtt
    24481 tggtttccca agcggatttt gtgctttatt ttgaaacttc ttttgcaaga acaaagttcc
    24541 caagtgtggc agaaccattt cctgcgactg ctggcgtcac gatatagtca cgcacatctg
    24601 gtactggtag gtaaccatta agaagagatg taaatttctc acggacacgg tccagcatat
    24661 gttgttgagc catgacccct ccaccaaaga caatcacgtc tgggcggaaa gtcactgtcg
    24721 cattaaccgc agcttgagcg atatagtagg cttgaacatc ccaaacaggg ttgttgagtt
    24781 caatagtttc cccacgtaca cctgtacgag cttccaaact tggaccagct gcataacctt
    24841 ctagacatcc cttatggaaa ggacaaacac ccttaaactc tttttcaata tccattgggt
    24901 gtctagcaac ataataatga cccatttcag ggtgacccac accaccgata aactcaccac
    24961 gttggatgac gcctgcaccg atacctgtac cgattgtgta gtaaaccaag ttttcgatac
    25021 gaccaccagc attgttacgg gcaaccattt caccgtaagc agagctgttt acgtctgttg
    25081 tgaagtacat tggcacgttt agggcgcgac gaagggcacc aagcaagtcc acatttgccc
    25141 agtttggttt tggagtcgtc gtgataaagc cataagtttt tgagtttttg tcaatatcaa
    25201 tcggcccaaa tgaaccaact gcaagaccag caaggttatc gaattttgag aagaactcaa
    25261 tggttttatc gattgtttcg attggagttg ttgttggaaa ttgtgttttt tctacaacgt
    25321 taaagttttc atcaccgaca gcacagacaa actttgtacc gcccgcttcc aagcttccat
    25381 ataattttgt catgataaac ctcttgtttt tattttcttt attatagcat acttcgaaag
    25441 tctaaatgtc tctatttttt agattttcct ctgtaaatct tactatctaa taaaaacgaa
    25501 caaacatgtc atttgttcgt tttcacatta gagaggattg attagatttt cacttcgatc
    25561 acagcattcc ccttcgcaac tgaacctgtt gcgactggag ctactgaagc gtagtcagct
    25621 gtatttgtaa cgataaccat tgttgtatca tcaagtccag ctgcagcgat tttgtttgag
    25681 tcaaatgttc caagaacatc gccagctttc accttattac cttgagcaac ttttgcttca
    25741 aaaccgtcac cgttcataga tacagtgtca ataccaacgt gaatcaaaac ttcagcacca
    25801 tttcttgttt tcaaaccaaa agcgtgccct gttggaaagg caattgaaac ttcagcatca
    25861 gctggtgcat agaccacgcc ttggcttggt ttcacagcga taccttgtcc catagctcca
    25921 cttgagaaga ctgggtcatt gacatcagca agagcgacaa catcaccaac gataggagtt
    25981 acaagtgttt cattttgaag agtatcagtc tcttccacta ctggtgcttc tactgtaggt
    26041 tctgtaactg tttctgcggt tgctgtggca gcttcatcag caaagacact agctgctttt
    26101 gttttcccgt agaaataagt gaaagcaaag gcggcaacaa aggacataat ttccgcaatt
    26161 atatacatag ggattgaacc agccttaata gaaaggaatc caataaatcc agccgctccc
    26221 aatgaaactg caataacgtg agttaaacct gctacaaagg caccaattgc tgaaccagca
    26281 agggcgcaga agaatgggaa tttgtatttc aagtttaccc caaagagagc aggttctgta
    26341 ataccaagga aggcagaaac agcagccgaa ctggacaatc cttttgtttt agcatttttt
    26401 gtcaagaagt agactgcaag agtagccgca ccttgtgcta cgtttgccat tgaagcaaca
    26461 acaaagatgt agtctccaaa tccagtaccg ttattattgt atgcagacaa caattgtgtt
    26521 tcgattgctg ggaatgattg gtgaagaccc gtcataacga ttaatgagta agttccacca
    26581 aatagcccca taccaaaggc acctgttgtg ttgtacaacc atacaatcgc atttgtaatc
    26641 gcatcagaca caaccaacat aacaggaccg ataactgtaa aggtaaggaa tcctgtaatc
    26701 atcactgata aaagcggtgt aaaagtgaag tcaactgcag atggcaattt cttgtggaag
    26761 aatttttcta aattagctaa caaccagact gcaacaagga ctggaataac ctgataagca
    26821 tagtttgttt gtgtaacatg gaaacctaag atgtcccaga aggcttcttt accacccaaa
    26881 tctggagtgg tcataatcat accgattgcc gcaccaagga attggtttgc tccaaagcgc
    26941 ttggctgctg aaataccaat caaaacaggg aggaacataa atggagcagc agacatcaat
    27001 tgaatcatag ctgagaagcc tttgatagca ggatacatat cttcaattga ttgaggtcca
    27061 aacaaatctg gtgatgtcaa gaagttccgt aaagccatca agagaccacc tgcaactaag
    27121 gcagggataa taggaacaaa aatgtctgac aaaagtttaa tcaagtccat caatggattg
    27181 aactttttgt ctttattagc aatctctttc aaatcatctg tcgaaacttc tttcaaacca
    27241 gttttcttaa taatttccgc atatacgaag ttaacatcac caggaccaat gataatttga
    27301 tattgtccgt tagtgctaaa agtccccttg acatccgcat tattatccag agctgcttga
    27361 ttcacttttg cttcgtcttt caataccaag cgaagacgtg tcgcacagtg agctgcagct
    27421 acaagattat ccttaccgac agcctcaatg acctcggcag caactttact ataatccata
    27481 gcaaaaatct ccttatataa cattttgttc ttgtttgaaa gcgattttat tcgcccttac
    27541 gactattatt ttatcatgtt ttaaaaatat gtcaagcgtt ttgcagaaaa aatattatat
    27601 ctacttagtg ggcaagcttt agattgattg tttttgactg tttcatcagg aatttcttga
    27661 aaaataagtt tgaaaccttg gttaaaaagt cttttatttc attatctttc acattttcgt
    27721 aaaaatttga ttgatttcct gtatttattt tatgataatc gtttgacagt tttttctctt
    27781 ttttaacata aagggcttgc attttttatc gaaaacagtt actattaaaa gtgataagat
    27841 ttttggagga atgaatggaa tggacaactg agcgtcgtta cagactttat caagattgga
    27901 cgcaagaaga aattcaacat ataaaggaaa atatggcaca atctccatgg catactcatt
    27961 accatgttga gccaaaaaca ggacttctca acgacccaaa tggcttttct tactttgatg
    28021 gcaagtggat cctcttttac cagaattttc cttttggtgc agcccacggt ttaaaatctt
    28081 gggcacagct agaaagtgat gatttgattc actttagaga aactggagtc aaagttttgc
    28141 cggatactcc attagatagc cacggtgcct actctggttc tgccatgcaa tttggcgata
    28201 acttattcct attttataca ggaaatgttc gcgatgaaaa ctggatccgt cacccatacc
    28261 aaatcggtgc tttgatggac aaggagggta agattacaaa gattgacaag atcttgattg
    28321 accagccagc agactctact gaccacttcc gcgatccaca aatttttaac tttaaaggtc
    28381 aatactatgc tgtcgtcggt ggacagaact tggagaaaaa aggcttcgtc cgtctctaca
    28441 aggctgtcaa taacgactac acaaactggc aagcagttgg cgaccttgac tttgctaacg
    28501 accgtactgc ctacatgatg gaatgtccta atctggtctt tgtagaggaa cagcctgtcc
    28561 ttctctactg tccacaagga ttggataaga aagttctaga ctacgataat atctttccaa
    28621 atatgtataa gattggggct tcctttgacc ctaaaaatgc caaaatggta gatgtgtctc
    28681 aacttcaaaa catggattac ggtttcgaag cctatgcaac tcaagccttc aacgctcctg
    28741 atgggcgtgc tctagcagtt agctggcttg gtttgccaga tgtttcttac ccatctgact
    28801 gttttgatca ccaaggaacc ttctctttgg tcaaggaact cactatcaaa gacgacaagc
    28861 tctaccagta tccagtcgct gctattaagg accttcgtgc ttctgaagaa gccttctcaa
    28921 accgttccca aaccaagaac acttacgaac ttgaactcaa cttggaagct aatagccaga
    28981 gcgagattgt cttacttgct gataaagaag gtaagggact ttcaatcaac tttgaccttg
    29041 taaacggtca agtgacagtg gatcgtagcc aggctggaga acagtatgcc caagaatttg
    29101 ggacaactcg ttcttgccct atcgagaatc aggctactac tgctacaatc ttcatcgata
    29161 actctgtctt tgaaattttc atcaataaag gagaaaaagt attttctggt cgtgtcttcc
    29221 cacatgcgga ccaaaatggt atcctgatca aatctggaaa cccaactgga acttactatg
    29281 aattagatta tggtcgcaaa actaactgat gtcgccaaac ttgcaggcgt cagtcctact
    29341 accgtttctc gggttatcaa taaaaaaggg tatctatctg agaaaaccat ccaaaaagtc
    29401 aatgaagcca tgagagaatt gggctataaa cccaacaacc tggctcgtag tctgcaagga
    29461 aaatcagcta agttaatcgg cttgattttc cccaatattt ccaatgtttt ctatgcagaa
    29521 ttgattgata aattggaaca ccaactcttc aaaaatggtt acaagaccat catctgcaac
    29581 agtgaacatg attctgagaa ggaacgcgaa tacatcgaaa tgttggaagc caatcaggta
    29641 gacggcatca tttctggtag tcacaaccta ggaatcgaag actacaatcg tgtgacagcg
    29701 ccgattattt cctttgaccg aaacctatcg ccagacatcc ctgttgtctc ctctgacaac
    29761 tatgctggtg gggttcttgc tgcccaaacc ttggtcaaga caggtgccca gtctatcatc
    29821 atgattacag ggaatgacaa ttccaattcg ccaaccggac tgcgccacgc tggttttgca
    29881 tccgtactcc caaaagctcc tattatcaat gtttccagtg acttttctcc cgtcagaaaa
    29941 gaaatggaaa tcaagaatat cttgacccgg gaaaaaccag atgccatttt tgcttcggat
    30001 gatttgacag ctattctggt cattaaaatc gctcaagaat tgggcatttc tgtcccaaaa
    30061 gagctcaagg tcatcggcta tgatgggacc tactttatcg aaaattacta ccctcaattg
    30121 gctactatca agcaaccttt ggaagagatt gcttgtctca ctattgatct tctcttgcaa
    30181 aagattgaag gcaaggaagt cgccacaact ggttacttct taccagttac gctattacca
    30241 ggaaaaagta tttaaacaca agaaaactca gaccgattcg tctgagtttt tatgatctta
    30301 aattttcgag atagcgctgg gctgtctcta ggttaaaggt tttatctgag atgaggcgct
    30361 ctactagggg agcaacttca gattcactag ccccagctag gagagctagg gatttggcct
    30421 gtagtttcat gtggccttgc tggatgcccg tacttaccaa ggctttgagg gctgcaaaat
    30481 tttgagcaag accgatggac acgataatct gggctaattc tctggcagaa ggatttccta
    30541 gtagatcatg actgagagct acacgtgggt tgaggccgat agagccaccc ttagtcgcta
    30601 caggcatggg cagggtcatc tcaccgacca attcttctct ttcaaggtcc agcgtccagc
    30661 agctaagacc ttgatagcgt ccatctcgac tggcaaaggc atgggcccca gcttcgatgg
    30721 cacgccagtc attaccagtg gcaatcaaaa tcgcatcaat accattaaaa attcctttat
    30781 tatgagtagc agctcggtaa ggatcagcct gcgcaaactg actagccaac gcaattttct
    30841 ccgcaatctc tcgtccttga tccttttggc ggctcaagta gcgaaaggcg atgcgacagc
    30901 ttgcagtcac cagagaatca gtcgcgtagt tggacaggat tcccatgaga ctctgtccct
    30961 gactgagttc ttctaagact ggtttcaagg cttccagcat ggtgttgagc atattggcac
    31021 ccatggcttc ctgggtatcg acatgaatat aaacaacgag aaagtctggt tcgcctttta
    31081 tctgctcgac atgcagatca cgcgccccac ctccacgttt aacgatagaa ggataggctt
    31141 gattggcaag ctccaagagc tccgctttct tgctggcaat cttctcttgc gctagtttag
    31201 gattagcaac ttgataaagg gctacctgcc caatcatctg tcgctgatgg acttgtgcag
    31261 taaaaccacc tgcacgcttg atgattttgc tggcatagct ggccgccgca accacagagg
    31321 gttcttctgt cacgtaggga acggtgtatt cctgaccgtt gacaagtacc tctggaacca
    31381 gtgaataagg cagagaaaaa gttcccacta cattctcact cagctggtct gccacagtca
    31441 cgctcatctg ttcatccttc tccagactag cttgtctctc aggactaagg agcgcctgag
    31501 cttttaacag ctcgaggcgc tcttggtatg attttttaga aaatccattc caacttatct
    31561 tcattatttt tcaaccttgc tataacggcg ttggtggtcg agaatttcaa ccaaggcaaa
    31621 atcttgattt tcatagccag caaactgggc agagtttgtt tcatccaagt ttacttcctc
    31681 aaaaaagacc ttttcatagt ctgcaacgga tagggcagtt cgttggttga gcttgttcaa
    31741 acggtcttta ttcaaataag cttcatatcc ttcaaccaat tcaccactga agaactcagc
    31801 cacagctcca cttccgtaac tataaagggc gattttatcc ccagctttca agctatctgt
    31861 attttccaag agagacaaaa gtccaaggaa aagtgaacct gtgtagatat tccccacctt
    31921 ttgactgtag agaatagact ggtcaaaatg cttttgtaag aggtcttttt tctcttgagg
    31981 caggctctta tccatgattt ttttcaagcc ttttagcgct aatttaggat aaggcaagtg
    32041 gaaacaaaca gccgcaaaat catccaaagt aagctggtag cgtttttgat attcaagcca
    32101 agtcgttttc aaactatcca agtattgttg ggtagaatag acaccattta cataaggagt
    32161 tgtcgagtaa tttggtcgcc agaaatccat gatgtcacgg gtctgagcta cattgtcatt
    32221 attaaaggcc atcatgcgtg gattttgtgt aatcaacata gctacacttc cagcaccttg
    32281 agttggttct cccggagttt caataccata tttggcaata tcactggcaa tgaccaatac
    32341 cttggactcc ggagaatttt ccacatgcaa tttggcataa tggagggcag ctgtcgcccc
    32401 atagcaggct tctttaatct cgaaactacg agcaaagggc tggatgccca gcaagccatg
    32461 cacaaagacg gccgcagcct tactctggtc aattcctgac tcggtagcca caatgaccat
    32521 gtcaacttct tgtctttctt gctcagttaa aatagagtca ctagcactgg ccgccaaggt
    32581 cacgatgtcc tcagttaggg gcgcaatact caattccttg agtaagagtc ctttacttaa
    32641 tttttcaggg tcaattcccc tcgcttctgc taagtcttgt aatttcaaga catattgact
    32701 ggtcgcaaaa ccaatcttat caataccgat tgtcatattt acctctgttt tatcattcat
    32761 gtaaaaaatc gttctatact attttatcac aaatggcagt aaaagagaga aaaaagactt
    32821 gattcaccaa atcaagcctc ttattggtca tcattttaaa gaatgattag ttgctagaga
    32881 gttcaccgat ataagtagct ttataagctc cattcacagt tatcagctcc tggaggatca
    32941 aatttcctga gtaaatcctt cccatctcat ctacaaattt ttgataaaac tgactggtcg
    33001 gaatttctct gacatcctta tcaaatgtct tgtcaagtgt tttactaacc ttctcagcaa
    33061 tcaattgatg ctcttgccat ccactttgaa actctgagcc cgaactagaa accatgactg
    33121 ggataaacaa caaggtcagt agatttacag acaataagga aagtagtaga cttcctgcaa
    33181 aactagaatc ctagttcatg attgataatg ccagcaatca aattcattcg taatccgaag
    33241 cgtttacgat gatttcgata ggttgttgaa aacattttaa acgtttttac tttggcaaag
    33301 atgttctcaa ccttgcttct ctccttagat agcgcatggt tataggcttt atcttcaact
    33361 gttagcggct tgagtttgct ggatttacgt ggagtttgtg cttgaggata tatcttcatg
    33421 agcccttgat aaccactgtc agccaagatt ttaccagctt gtccgatatt tctgcgactc
    33481 attttgaaca acttcatatc atgacaatag ttcacagcga tatccaaaga aacaattctc
    33541 ccttgacttg tgacaatcgc ttgagccttc atagcgtggc atttttttta ccagaatcat
    33601 tcgctaattg ttttttttag ggcgattgat ttttacttcc gtcgcatcaa tcattaccgt
    33661 gtcttcagaa ctgagaggag ttcttgaaat cgtaacacca cctttgaaca agagttactt
    33721 caacccattg gctccgacgg agtaagttgc tttcgtggat accaaaatca gccgcaattt
    33781 cttcataagt gcggtattct cgcacatata gaaagcgttg ttaatttatt tatctcattt
    33841 ttcagaaaat tcttttattt ctgtaaagtc tacgatactc gatgtgtttt tatataatga
    33901 tacagtctga gaatcactgt tccgctagcc attccaatag agattaccaa agccaacatg
    33961 acaaccaagg tcgcacttgc cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt
    34021 gttcggtagg agagataacc tggaaccagc ggtgccaaaa tggccaagat aaagaccaca
    34081 gcaggtgtct tataaagaat acttaaaatc tggctgacac aagaaccaat aatggctgca
    34141 atgaaggtag ctacaatgac attggtcggt tccttgagca agagatagat tagccagaca
    34201 gtcatgccca aaatccctcc aggtaagagc atagaccgtt gcacattgag tacgattaaa
    34261 aaagtgataa tggcaagaaa acttgctact gcttgtaata aaaaggttgt tagtgtcata
    34321 ttagttcatc aataccaagg cgacagaagt tcctgcccct aaagcgaggg taatgagcag
    34381 ggattcaaac atcttactca taccagagtt tatgtggttg gtcataatat cacggaccgc
    34441 attggtcaag gcaatacctg gtacaaacgg catgaccgca ccagctataa tcaaatctgc
    34501 cgttgaagga aaacctgtgt agcgagccca aaactgggca attatcccaa agacaaaggc
    34561 tccagcaaag gctgtcacaa agggaattcg gataaatttt tccacataga gggaaaaggc
    34621 aaaaccaaat aaggtcgcca ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat
    34681 aactgaaaag aaaggagcac taaaggtcgc agccagagtt acctgcaact tagtataggg
    34741 aaggggttga gcttgcaagg ccgtcaattg cttaaaggct gtttctaagt caatctgccc
    34801 cccaactagc tgacgagaaa tctggttcac atcgcagact ttttcgatgt tataagaaga
    34861 ggaggtcacg cgcttcatgc gcgaaatatt ggtattttca atagagaaaa agatagcggc
    34921 aggcatggca aggacattgc aatccacaat cccctgcgaa tgcgcgattc gaatcatggt
    34981 atcttctaca cgatggattt ctgagccact tttaaggaga atagtccccg ctagcataat
    35041 cacatcaatg acggcattta attcttttga ttcttccatg ctttcctcct tttatcaact
    35101 ccctctattc tatcacaaat ctggactcaa aaaaaaatct ttgccatgaa atcatgacaa
    35161 agattgatta ctcattttga ttatccatct gcttttaagg agtagctgaa gttgttttag
    35221 gtttgtagat tgaaatcttg actctagtct tatttaggtc taccttttca cctgctctag
    35281 gactttgttc aacaaccatg ccttctacac tacctgcagg cgctgtcgtc acttctacaa
    35341 cttctatatt agcttcctta atcccaacaa tttgaatcaa attgttctta gtaaactcca
    35401 agctggaacc aatgtaactc ggcatggcaa cacttgtaac ttttttagct actgtcaaga
    35461 caatttgagt aggtttactc acatcataag tcgttccggc acctggactt tgtttcataa
    35521 tcgttcctgg ttcgctttcg ctggactctt cttcctctat cttaatcaaa ttctcaggaa
    35581 ccttcttctg cttgagttct gagattactt ctgtagagtt ccgtccaata tagttcccta
    35641 attgaatcgt cgtagctttt ttagctactg tcaaaacaat ttgagttgcc ttactcaagt
    35701 cataggtcgt accttctggt agactttgct tcaggaccgt tccagcctca ctctcattcg
    35761 actcttcttc ctcaatttta atcaaattat ctggaacttt tttctctttt aattccgcga
    35821 taacatcaga agatttccgg ccgacataat tactaatttg gaaggattgt ttgcctgatg
    35881 agacaaccag attgattttc gttccttctt ttcgaccagt tccagcgcca ggatctgtac
    35941 ggataatccg cccttcttcc accttttcac tagcctctgt cttctcctca ccaatctcaa
    36001 aattggcttt tttgagcgtt gccttggcct ctgcaactgt ctgacctgcc acatctggaa
    36061 tggcaatggt tgcaggagtt ctggatagta tccaaataag agaagctgcc accaatacaa
    36121 ggctggccaa caaaatcagg taacgcatct taaatctatg ttttttcggt gcttgtggtt
    36181 ggtaagtttc ctctgtcaca gcctggcttg ggtttttgat tgatttgtgt tctgtctgcg
    36241 cttgaacctt aggaatagat gtcaaggtac tctgagaaac cttcggcaag gtcttggtat
    36301 ctgccttgct cgtttcatca aagattaact tactttcatt tctacgattg taggacaagc
    36361 tactagacaa gtccacatac atctctgaaa ccgagcggta gcgattggtc aactttttag
    36421 cagttgcctt gataataaca ttttctaaag cctgaggtac agatggattt tctgcaataa
    36481 cggacggcag gggtttctgg aaatgctgga gggcaatggt caccgcgcta tccccgtcat
    36541 aagggatatg gcctgtcaac atctcataga aaataatccc catggcatag atatcactct
    36601 gcacagtcgc cttcgaacca cgcgcctgct ctggtgacaa gtaatgaact gagcccaaca
    36661 tcgagttagt ctgggtcaga cttgtctctg caaaggctac agcaatccca aagtctgtga
    36721 ccttggccgt cccatctggt gtcaaaagga tattttgagg tttcaagtcc ctgtgaacaa
    36781 ttcctcgagt atgggccaag cgcatagcca agagaatttg tcccatgata cggactgctt
    36841 cttcattaga aagaggataa tgttccttga tatagcgttt gaggtctagt ccagcaacat
    36901 actccattgc aagatactgt tgaccgtctt cctcaccaat atctgttatc cgaacgatat
    36961 gaggatggtc tagatctgcc atagctctcg cttcacgctg aaaacgagct acagctatcg
    37021 ggtccgtctg gtagttggtc ctcagaacct tcactgccac ttcttcccca tctaagatta
    37081 agtctttggc taggtagaca tccgccatgc ctcctcgacc aatctgtttg acaatccgat
    37141 agcgtccggc aaaaatcttg ccgatttgga tcattcttca tcctcctcgt tcatagaaac
    37201 aagggcaacc gtaatgttgt ctaaacctcc tgcattgtta gcaaaacgaa caagtgtctc
    37261 cgttttatct gctaaaggaa tatcactggt tacaatatca cgaatctcac tgcctgaaat
    37321 catgttggtc aagccgtcac tattgagcaa gagatagtca cctgactcaa ggataactgt
    37381 cccaaaatca ggctgaattt catctttttg cccaatagac tgggtgataa tatttttttg
    37441 cggatgagct tctgcctctt ctggtgtcaa ttgaccagcc ttgagcaatt cattaaccaa
    37501 ggaatgatcg ctcgtcaact gatggtattc ttctccacga atcaagccga tacgcgaatc
    37561 accaatatga gcatagatag cctgattatc aataatagca aggacttcca aagtagttcc
    37621 catacctctg taagcttcat cctgaccaag ctggtgaatc ttttgatttt caatttctag
    37681 gtaatgggcg aaccattcac gcacttcatt gactgtatcg atctgggtat caacccaagc
    37741 tacacccagg tctgtgaccg ccatttcact agcgatattc cctgcacgat gacctcccat
    37801 cccatcagct aaaataatca tggtacgtcc agctctattg acatagtggt tgacatagtc
    37861 ttggttattt gttcgtttct gaccaacatc tgttaataat gaaatttcca tgtgtcagtt
    37921 ccttcctaat ccgatatctt gcgaaattga ctgatgaaga atccatcact tccatacaat
    37981 tcaggtgtaa tgaggataca gccgtctttc atgatatcct tacattcatg ttctagtttt
    38041 acctgctcga actcgggatg actctctaaa aaggccttaa cgacttgaaa attctcctct
    38101 gagacgatag tgcaggtgct ataagttatt ataccacctt tgcctagtat ttgacaaaca
    38161 ctacctaata tttctaactg aatttcctgc aaggacgcga aatctgccgt ttctttattg
    38221 tatttgatat ctggttttcg gcgcaaaaga ccgattcctg aacaaggagc atccaccaaa
    38281 atcttatcaa aggaatcctg gtcaaaaaac tcatgcacct ttctggcatc taatttttga
    38341 gtttgaaccc gatctgcaac tcccagacgt tgggcatttt cttggattaa atccaacttg
    38401 tggtcgtaca agtccagagc agtaacctga cctgtcgtaa gataagaggc tatatgggct
    38461 gttttcccac ctggagccgc acaggcatca agcactcgct catcaccttg taaatcaagc
    38521 gtcggagcaa ccagctgact ggactcgtct tggatggtaa tggctccatt cgtaaacaaa
    38581 ttatgccctg caaaatgccc ttgctcctta accagaccag tggttgctaa aagggaatta
    38641 ttcgcctcca acaaggcttg gatttcctct tttcgactta ggtctgttac acgaatactg
    38701 gctttgtttc gcactaacag gctttcaaag atggcttttg ctctctcctc tccgtattct
    38761 tccttgagtt tggcaactag ccaaactggg agagaatagg caatggagtc acgcttgttt
    38821 tttcgcttga tgctagcaat atctggccag ccttcacgca agatacggcg aaggacagcg
    38881 ttgaccaatt tttcactgcc ttttttacgg agtttggcca attccactgc ttcattgacc
    38941 acagcatgat ctggaatctt gtccaaatag cggagttggt aggcactcat gagaagaagg
    39001 acatagagcc agctgtctaa ctggtctctg tcttcgataa agtgggatag gtaccattcc
    39061 agagtcagtt tacgggctac cgttccatag accagctcgg tcactaagcc cttgtctgct
    39121 gcagagagct gactcccctt gaggtgttta tttaaggcaa tatttgaata tgcttgattc
    39181 acaaaaacat cctctagcac tgctagagct aaacttctag ccgtttctac tttagtcacc
    39241 aaatcgttct cctacagtca atgtacgtcc aactccgttg aggaaggaag caatgtccat
    39301 cttaggctta ccagctggct gcacttgttt gagggataga gccccttcag ccgttgcgac
    39361 aatcaattct ttcttgccga tagagagaat ctcacctgga tttccctgac cttctactgg
    39421 tagggcttca taaatcttaa agcggtcacc cttaaggaaa gtatgggcaa caggccaggg
    39481 gttcattcca cgaatttggt taaagagttg acgattggtt ttgttccagt tcagtttttc
    39541 ttcctctgac tttatatttg gagagaaggt aacctgactc gtatcctgcg gttcaggttt
    39601 gatatcacca gcaatatagg caggcagagt gtccaaaagc aaatcacgac caactagcgc
    39661 caatttttca aacaaggtgc caacattgtc ctcatctgtg atcggaatgc tgcgacgaga
    39721 aatcatatct cctgcatcca tttccttaac catttccatg atggtcacac cagcttcctc
    39781 atccccttga atcaaggcat aatggatagg cgcaccacca cggtgtctag gaaggaggga
    39841 ggcatgaacg ttgacagcaa agtccatgct atcaaggagt ttgcttggga gaaactgccc
    39901 aaaagcagca gtcacaattc catctgctcc tagcttcata agatcttcca tctctggact
    39961 tccagataat ttttcaggtt gatagataga taatcctgct tccttggcag cctgcttgac
    40021 tggggtttct tggataactt ttttacgacc aacagcacgg tctggctggg tcacaacggc
    40081 tagaatttcg taacggtcat ctgtcaaaag tccttttaag actgttgctg aaaagtcggg
    40141 ggtccccata aagattagtt ttgtcatatc ttctccttct tataaaaatt gctgcggctc
    40201 atggtcaatg ctgagacgga gctcactatt ttcccgttct tgagtcaagg ccaggacctg
    40261 gttgagggtc ggccccagct catcttctaa acggtattta attaaaatct ggtaatgata
    40321 gaggttgtgg gtacgggcaa tcggttttgg cgttggcccc agaatgggac tggtctctga
    40381 caagcctgac cgcaaaatgt tcatgacttc ataggcacgt ttgaaaacct cttcttcttt
    40441 cttgtgagaa agggtaatac caatcgtgaa atagtaaggt ggatagccga gttgtcgtct
    40501 gattcccatt tcataggcat aaaagccttc gtaatcttga tccttggcaa atcgaatagc
    40561 atagtgctgc ggattgtagg actgtatcaa gacttgacct gctttttcag cccgacctgc
    40621 tcgacctgcc acctgagtca agagctggaa ggttctctca gaagaacgga aatcaggcag
    40681 attcaaggcc gtatccgcat ttagaactcc gactagggta acattgggaa aatccaaacc
    40741 ctttgcaatc atctgagtac caagtaaaat atccgcttcc cctcgcccaa actggtcaag
    40801 caaggcttgg tgactgcctt tctttcgagt cgtatccaca tccatcctca aaatgcgagc
    40861 ttggggaaag agttctgcta gctcatcata agccttctga gttcccgtcc catagtaacg
    40921 aatactgtgg ctcttacagt taggacagac ctgaggaata tccttcgaga aaccacaata
    40981 atggcagttc atagtcttgg tatccatatg caaggtcaga gaaatatcgc agttgggaca
    41041 agtatccacc gtcccacact cccgacacat gacaaagcta gaataaccac ggcgattgag
    41101 catgagaacc atctgctctt ttttaaccag acggtcttgg atagcctcta gcaaaggagg
    41161 cgtaaagttt gacgtctcat tttgtccgat atagtctcga aagtcaatca cttgaacctc
    41221 agggattgta gccaaaggat tggcacgttg ggttagacgt aagtgttgat agacgccttt
    41281 gccagcacgt gcccggctct ctaagctcgg cgttgcagat ccaagtacca gagttgcttg
    41341 attatactga gcccgtaaaa tagctacctc tctggcatgg taacggggat tgctgtcctg
    41401 cttataagcc gcttcatgct cttcatcaat aatcatgaca cccagatttt tcagaggagc
    41461 aaagatagca gatctggcac caacaacaac ttgggcatcg ccacgctcca ccttgcgcca
    41521 ttcatcatac ttttcaccat tggataatcc tgagtgaaga atggctacct tgtccccaaa
    41581 acgtgctata aaacgctcgg tcatctgagg agtcaaggaa atctcaggta ccagcaaaat
    41641 agctgtcttg cccttatcca gggcaccttg gataatctgc aagtaaacct cggtcttccc
    41701 acttcctgta atcccttgaa gtagaaaggg aggttgagaa ctgccaatag aactcacaac
    41761 cgcatcacgc gcctgtcttt gttctggatt taactccaaa ggtctacttg cttcaattcc
    41821 ttcaaaataa gcagccgagc gtcgaacttc cttttggact atagtaacag caccttgatc
    41881 cacaaagaag ttaacttgct ctcgcgagta ggactctaac aagctagcca aggaagcgct
    41941 ctctggatga gacagcagat aatctctcag ttccaacttt ttcttggcac gtgtagaaat
    42001 ctcaacacct tctaattgag catggtcaac ctcataccaa gactgggtct tgaccttctt
    42061 ttgatcgact gcctgatatt ccagaccaag caggcctttt ctagtcaaac gcatcatttc
    42121 agcttgctta cctaaatcca gagaagaaaa ggctagcgaa tcttctgaat caaacaggcg
    42181 tactcgttct tcctgactca agccttccag aggatagaga atcttgtcat agctagaatt
    42241 cagaaatccc ggaagcatgg ccttgaggat agagattttg taggagaaga cagatttgcg
    42301 taactcctca gccagccaga gttgttctgg cgtgagaaca ggagaaaaat ccagcacctc
    42361 tgcaatatct tttaaatctt gctccatctc ttctccatct gattgggact ccaaaccaag
    42421 aacaatccct tgaatcaggc gattaccctt accaaaaggc acatgaaccc gcatcccaac
    42481 ttccagcatt ccctcaaatt cctccggaat cctgtaacta tagggctggt ccgtctgcat
    42541 caagggcaca tctacgataa tcttagctag ggccatcttc tcacctcctc cttgtcagta
    42601 cattcttgca atagaaaaaa taagattgag tccccccaac cttaaatttt ttcaccatct
    42661 tctttttctt tagcaatttg ctctttgatt ttcttttctt cttcttcttt gcggcgtttt
    42721 tcttcttcga tacggcgacg cactgcttca cgttttcctt ctggatctgg gtgaattgta
    42781 acgtttcctg attcgatttc ttctaaagcg cgaagagttg atttttcaga cttgaaacct
    42841 tgagttgctg gggcacctgc ttccaattcg tgggcacgtt ttgcttccaa gattacgagt
    42901 gaatattttg aaggaacctt gtcgagcaag gtatcaatag agggttttaa catcatttgc
    42961 ttgtacctat tttctaaatt ttatcgggta gttggagatt ttggtaacat ctcctgatag
    43021 tgaccaatga cacgatccac acagaagtgt tctgcttcaa tcacacattt gacacgttca
    43081 gcagctaggg gcacctgatc gttgacaatc gcataatcat actcacgcat gagggcaatt
    43141 tcttccttgg ccttttcgat tcgttgggca atcacttctg cactatctgt tccacgacct
    43201 accaagcgat cttgcaattc atccaaatct ggtggtgtca ggaagataaa gacagcatct
    43261 ggaacctttt tcttgacctg aagagcaccc tgaacttcaa tttcaaggaa aacatcgatt
    43321 cccttgtcca aggtttcatt gacataggtc agaggagttc catagtagtt gccgacatat
    43381 tctgcgtatt ccaacatctg tccttgacga atcagctctt caaattcttc acgagtacgg
    43441 aagaaatagt caacaccgtc cacttctcca ggacgttgtg cgcgtgtcgt catcgataca
    43501 gaatattgaa attggttttc agaactctca aaaatctctc ttctaaccgt tccttttcca
    43561 acccctgaag gaccagaaaa aacgattagt aagcctcggt ctgccattgt gtctcctttt
    43621 agtcaatctg tgaaataaca tttctctaga ataatggcaa aaagccagat tatcctttac
    43681 agtctttcta tctagtgtaa caaaaaagca gtaatttttc aactgctctt tcttatttat
    43741 ttagcataat ctactgcacg aagctcgcga atcacggtta ccttgatatt tcctggataa
    43801 tcgagattgt tttcaatttt cttacgaact ttgtgagcca agattgtgac tttgtcgtcc
    43861 ttgatttttc ctggattgac catgatacga atttcacgtc ctgcttgaag ggcaaagcta
    43921 gtttgcactc cttcaaagcc gttagcaatt tcttccaaat catggagacg cttgatgtag
    43981 ctttcaagag actcactacg agcacctgga cgggctgcgc tcaaggcatc tgctgcagcg
    44041 acgataactg ctatcacgct ctcagcttca acatctccgt ggtgactagc aatcgtattc
    44101 accacaactg ggggttcctt gtacttacgg gccaattcca taccgatttc aacgtggcta
    44161 ccttcaacct catggtcaat ggctttcccg atatcgtgaa ggaatccagc acgacgggca
    44221 agagccgcat tttcaccaag ttcgctcgcc atgataccag ccaacttagc aacctcaatc
    44281 gaatggcgca aaacattttg tccatatgaa gtacggaact gcaaacgtcc cataatcttc
    44341 atcaagtctg gatgaaggtt tggcgcacca atttcatagg cagcagcctc accgtattca
    44401 cgaatcttat tgtcaatctc ttgacggttt ttctcaacca actcttcgat acgagctgga
    44461 tgtatacgac catctttgag caacatttcc atagtcatac gggcaatctc acgacgaatc
    44521 ggatcaaatc ctgacaaggt caccacttct ggtgtatcgt cgataatcac atcgacccct
    44581 gtcaaacttt caaaggtacg aatgttacga ccttcacgac caataatgcg tcccttcata
    44641 gtatcgtctg gcagatgaac tgttgagttt gttgactccg ctacatattc accagcgata
    44701 cgttgcatag cttgaaccaa gatgtccttg gccattttgt cagaacgttc cttgacctct
    44761 tgctcagctt cgcgaatgcg actggcaatc tccctggtca agttttcctc tgtctgagcc
    44821 aagataatat ctcgtgcttc tgcctgagac agagcaccaa tacgctctag ttctgcttct
    44881 ttttgtcttt cgacttcctc taattgctct tcacgcgcat caaggttttt cgctctatca
    44941 gaaatacttt gttctttttg ttcaagtgtt tgttctttac tcgtcaaatt gtcgtcctta
    45001 cggtcaaggc tagtagctct ctctgtcaaa cgactttcga tttgtttgag ttcttgacgt
    45061 tctgatttga attcagcgtc cacttcttca cggtattttc tggcttcttc tttggcctcc
    45121 aatagtgctt cttttttaag agacttgctt tcacgtttgg cttcattaac aagtaaatcc
    45181 gcttcacgct cagcttgtcc acgtaaatta gttgcttctt gttcagcatt taaaagcatc
    45241 aactctgcag cttcctgaga tgatttcatc ttagctgaga tgctgacata tccaatgact
    45301 aaaccaatga tgacggcaaa aacagcaatc gcaagcgaca tgatttccat gtttttacct
    45361 cattttattg ttattccgaa tgacatacat tcttttacat tctaccataa aaaagtgatt
    45421 ttcacaaacc taaaatagaa tatgttttgg ggaatttgga acacatttgc caaaataaac
    45481 ttgtttttta gaaatagtag tttagtagag acttgagaaa aagcctacct ttcaatagac
    45541 ttagtaatga tctttaaagg acaagaaagc cacgctatct ccatccatca tataaatcaa
    45601 gcgattttct gcatcaatac gccgtgacca ggctccttgg taatcatatt tgagtggctc
    45661 tggttttcct attcctgtaa atggatcgcg ttgaatatcc ttgattaatt tattgattct
    45721 ttttagtgtt tttttatctt ggttttgcca gtagcaataa tctgcccacg catcttctgt
    45781 aaacttgagt agcattcatc actcctcaat cacatggacc tgaatgcttc cagcacggac
    45841 ttgagccatt cctcgcaaaa ccttgtctga taactcctta ttttgagcga ttctcagggt
    45901 ttcctgaata ctgtcccatt cgctctttga aagaactaca atgtcctcat ctggattttt
    45961 attgaccacc gtcaaaggct caaattcatc atttaccttc ttcatgtagt cctttaaatg
    46021 atttcggaat gttgagtaaa ggactgcttc cataaccata cctcgtttta gctcttttcc
    46081 actattatac acgaaaagaa agaaattgtc aggaacttgt acaagatttt cttttctatc
    46141 tatttatact caatgaaaat caaagagcaa actaggaaac tagccgcagg ctgtacttga
    46201 gtacggcaag gcgacgttga cgcgatttga atttgatttt cgaagagtat tattcgtaaa
    46261 aaatctcaaa aagcctacct ttcggtagac ttagcttgtt tctattctaa tcggcactct
    46321 tccaaaattt tgctctgcta tacttggctt tcctagttgg taaatctggt ctgctttttg
    46381 ggtcatagca tcccaaggtt ctttgccaat tcggctgact agattgacct gtcctttcag
    46441 agacttgaga tgttgcctgc ctttttcagt aaatccaagg acatgaatgg cttctggcaa
    46501 gacattttct ctagcctgca ccaaaatata ggtcaatagg cgtctgacac gcgccttggt
    46561 gtaacgtttg gtcgcaaccg cctcgaccaa ctcttctaca gactgggctg ttttaatagc
    46621 ttctttaatg cgcacagcca tttcttgatt gacctgatag atggtggtta ggtcgggatt
    46681 tgacaagatt tgatagcgga gcaagggaaa atagtcttcc cagatcacct tactggcctg
    46741 ctcaaatagg gcaacagaag gcataaagcg ttctaagaaa tcttggtcct tctgatgttg
    46801 acggagggct gtcgccgagg caaagtccac atctttattc acagaatggt aacctgcccc
    46861 ctgacgctga atcgtatgca gcttgatatt tcgtcctgca accgcctttg cataggccag
    46921 agcaagaaca tgattggggg tattacctga aaaatcaaga ccagcaaatt ccttccacat
    46981 agattgggtt ttctggggat aggaaaggga atcaggcaga ttttccacaa atttctccat
    47041 ctcagcacct ttctctgtgt ataagtcagc gattttctga taatctcgaa cttcctctgt
    47101 cccaaaaact agactatcaa tgcccaagcg atccaagata tccacagctc cttgaccaaa
    47161 gaaatcagct gcctgaacac tgactaaaaa gggcaattcc actaccaagt ctgcgccatt
    47221 ttccagcgcc atctgggccc ttgtccactt atccacaata gcaggctctc cacgctgcat
    47281 gaaattccct gacatggcaa caattttcag tccctctgcc tgatccagca ggtatttatg
    47341 gccattatga aaaggattga actctgcgat aatacctgtg atggtcatga tgttctccta
    47401 cttctgcgcc acaaaaaacc aacgggtgct agtttctgtt ggttccttat cctcaaagtc
    47461 tgcatagagt ttgaaggact tgaatccagc ctgttccagc aagatatcat aggtcaagat
    47521 ctcataagtc ctctcctcat gtacttcatc gtggcgacta aaggaaccgt cagcctcctt
    47581 gataaagaaa gtcagctcat gcacgatgga gtgaggagct tcgtcctcat atgtatccca
    47641 aagcatggca aaatcttccg cattttcatg gtaggaatag ccagggaata cttcatctgt
    47701 ctggtaggtc gagtgcacgt caaagatgaa aactccctct tcattcagcg cattgtacac
    47761 gtccttaaag acgtccccta cttccacctc atcctgcata tagcagatag agtccgaata
    47821 acacgtgaca aaatcgtatt tccctgcctt ggacaaattc agcatattac cttctataaa
    47881 agcaatcttt tgcttggctg aagttgctct cttctccgca atcttcaaca tatccgcact
    47941 caagtcaagt ccagtcacat caaaaccagc ctgagagaag cgcactgatt gaattcctgt
    48001 cccacaagcc aattccaaga gtttctttct ctccttggtc ttaggcaaat gacgcagaga
    48061 aaaattcgtc catttgtcgt ataaactatc gtccatgacc gcatcataga ccgccgcaaa
    48121 ggtttcataa gtcgccataa tcatgatact aagagctcca tggtaaacac cactagccct
    48181 ttacctttct atcaattttt ctaaaataag caaagaaacc cagttgacta caactgagtt
    48241 catcttctaa gcaagagctt ctgaaatatc tactgaattc gcctcatgcc atagcttctc
    48301 tagattataa tgggcacgca tttcttctga aaagatatgc acgacaacag caccgaggtc
    48361 cagtaagacc cagcctccag ctgcatcgcc ttcgacatgg ctacctttaa agcctgcttg
    48421 agctactttt tcacggatat tagctgcgat agcgtccaac tgacggctat tcattgagct
    48481 agtgatgaca aagtagtccg tcacactagt caaatcttgt acatcaagtg cgaggatatc
    48541 ctccgcacgt ttctcatcag ccgctttcac gactagttct agtaattctt tttcgttcat
    48601 ttaatcctct ttttagaaaa tatgtttgta atctagaaca tctgcaaagc gctcctccca
    48661 aatgtcctca taaaattcat taatcgtttc tgctggaatg tcatccccat caaaggttgt
    48721 gacagcacct tctggaacaa taagctgata gccatattca aaggcaacct tgacagaggt
    48781 atccacacaa tattctgtct gcataccaca taaaactaat ttttcaatcc cctgcttatc
    48841 caagtattct tttaagccag tttctttgaa aatactgtta tacttcttct gaaagacctt
    48901 ttcagcaggt tttcgattta aaagcgcaga taactgccaa tcttctgatg tttgagcttc
    48961 agagttctca atatgttgaa catagataat ttcaatattc ttgcttctag cctggttttg
    49021 taaataagaa attttttcca atagactttt tgtctggaac ccagtttcta ctaaaacatt
    49081 ctgaacatca atgattataa aagccgtctt catttagtcc tctttcaaat agtgcacaaa
    49141 ggcgttatag gtttcaaggg tttggggata gatggggaat ccctgatgag ctagatactc
    49201 cacggtacga gctgtttcgt aggccaccgc cttattgagc gatagacttg caatctcacg
    49261 cgccacatcc actccaggaa atgcccgatt gtgctcgata tagtctgcga cgtagatgac
    49321 cttatctagg tctgtcattt gaccagctcc gactgtatga atttcaatgg ctcgcagaat
    49381 ttcagaatca tgcaaatcca aatcttcctg aattttgtaa attccgacca taccatgcca
    49441 gacattatta ccccagtttt tgaggtcgaa atctagctga taacggtcaa tcaagtctag
    49501 aaattcctga tctgacaact ttttagcata gtcatgaaga agacctgcta gacttgcttt
    49561 ctcaacatct acaccaaagc gctgagctaa ttccatagcg gcacgctcca cacctagaca
    49621 atgggttaga cgtttctcag gtagaagctc tgccattttt tccaacaagg cctcacggga
    49681 gcagttgata tagtcttgat aggacatcag tagagcccct ccttctcgat gtagtctagc
    49741 actggctgag gtaggagaaa gttgggtttc cgaccttggg caatgaagtc ccgcaccatg
    49801 ctggacgaga tatccatgag cggtacgtcc acccagataa ctggatagga agtccctgcc
    49861 ttgtagcgtg gacgctgaac ccccacaaac tgaaccatgt caaccagttc atcaattcgg
    49921 taccacttag gcagatagtc aaccatgtcg gcaccgatga taaagtaata atccgtatct
    49981 ggattcttct ctgtcaaaat cttcatggtg tcgtaggtgt aggaaatacc cttgcgctcc
    50041 aactcaatgg tttcaatgac taggccgtca attccctcaa ttgccaactc aagcatcttg
    50101 agacgatggt gttcagggat ggtttccttt ttatcaacgt gaggaggttg gtattcaggc
    50161 atgagaagaa cttgatccag tcccaactgt tgccgtactt gatccgcaac aatgagatgg
    50221 gcattgtgaa cagggttaaa attcccccct aaaatcccaa cttgtttgcg ttttttctcc
    50281 ttgatttctg gctccaactc taccttggta aagggagtca atagttcgat tgtcataggc
    50341 tagtcccctt tatttctcta taaaaacagt tatttggagt ttcggtctta gatttctttg
    50401 actttcttag aaatcttgcg attttctttc ttgctagatt gtttaaacaa aatcaagatg
    50461 cgtcctattt tttggactgt atccacaccg atttcttctt ccaaaatttc agctacttcg
    50521 tggatgtttt catctgtgtt ttgtaagaga gtaaccttga ttaattcacg cgcatcaaga
    50581 gcttgacgga cgctggtttt gatttggtcg ttgagtccat ttttcccgat ttggatgata
    50641 ggtttgaggg tgtgtgcctg gctgttgagg aaggcacgtt gttttgatgt taatgacata
    50701 atttctttaa aagagtttct ttttatactt ttctgaagtg gtgacggacg tcagcaaagt
    50761 ccttcggact ttcatgacta aaatttgagc ctaaggtctc aaattttccg cagttggtac
    50821 aatcacttgt accaacttac accacagcga aaagtattct ctatggggct cgccttgctc
    50881 gcccaaattc agacaaccct ttcctttctg tgcttaaata attgcttttc gtgtgacgac
    50941 ggcgacgcct tctggtgccc agacggcgac ttttgctatg cctgttacac gaatccagcc
    51001 taggcctgaa atgactaggt ctgtcttatc tttaatggta aagacatgct ggactagcct
    51061 tgggaaatct tctttttcct tgctatttgg tggtgtcaga agagttccca ggtgcttatc
    51121 gtagaaagca ctagctcctt caagcttgct acgatggagt ttgagttcat tatcaaagaa
    51181 agcagtaaat ccttgctttt ctcctgctat aaagtcaaag cgtcccaaac cacctaaaaa
    51241 tagggtttgc tcaggattaa gctgataggt cttaggcttg atttcctttt taggactgac
    51301 atacttgagg tttttggccg tcaagtagtg agccatctgg tggcggtgga taattcccgg
    51361 cgtatcgtaa atataagatc cgtcgtcaag cggaatctct attttgtcca aggttgtccc
    51421 tgggaagcgt gaagtagtga tgacattctg atcacccgtg atttcttgga taatagcatt
    51481 gattagagtt gattttccaa cgttggtcac accgaccaca tagacatcgc ggcccttacg
    51541 gtagtgttca atcttgtcaa tgacttcctt aatggcatgt ttattttgtg ctgaagttag
    51601 gaccacatcg actggacgaa gaccttcttc ataggcacgt ttcatgagcc actggctaat
    51661 cttaccagac ttaactgact taggaaggat atctttttta tttcctacca agaggacatc
    51721 attgcccgag acgaaacgtg gtaaacctgg gatgacagat ccattaaaat caaagatatc
    51781 aataacattg accactaaag catcactgtc tcccacctcg tgcaagagct tgaggaaatc
    51841 atcgttcgtc aactggacat ctgtgatttc attgtagtgg cggagacgga aacagcgttg
    51901 gcaatagact tcgccagtct ccaaaccttt ttcaagtgcc gactgggggg taaaaccaag
    51961 accagcttta tctgtcgtct gaatggttgc tccacaacca atacagagaa tttcttccat
    52021 agttaaattc cttttttata tgtaatcggt ccgtactttt cagtgatttt tctcataaca
    52081 cgacgctcac gagttcggtt aatctgcgtt ttgattgagt catgttggac caagggtttg
    52141 actaaaattg accgaatccc tgcacggtgg gctgctcgta tatctgtcat gagctggtcg
    52201 ccaaccatga ccacttcctt tttgtcatag tggaattcct tcatagcacg gtcaatacca
    52261 aatgtgaagg gcttcaaggc ccagtaaacg taatcaatcc caaatttctc aactgctcgt
    52321 tgaacgcgtt ttttggtgtt atttgacact acgataatgc caatacccgc gtcccgaagg
    52381 tcatgtagcc attgcttcat ctctggcgtt ccatcagggt tgttccaagc aatgagggta
    52441 ttatccaaat cgaccaaaac agccttgatt ccctgcgcct gcaggcttgg gactgtcaga
    52501 tcatagactg cttccacagc aaaatctggt atataatttt caatcgccat tctggctcct
    52561 tttcttaact ttttttcgca attttcctta gtaacaagga taaaacaatc cacaaacctg
    52621 cactagccaa actgatatag agccaagcat ggggctcatc tgttaaaggt aagggaacat
    52681 tcattccgaa aaagcctgtc acgactgcca aaacagctag caagactgaa atgatagtca
    52741 aggttgtcaa attgtcattc agattattgt ttagaatatt gttgtaagag gctgaaagct
    52801 gctgtaaaat ctgagagatt aggtctgtca tggataccag ctgatgagcc tcaatcatgg
    52861 catcatcaaa ctgttctctc tcaatctcat caaaactacg atacaaggca tgaccttgaa
    52921 tatgctctaa caaaatccga ttttgtttgg cagctgccgt cagataaacc ataccagtct
    52981 ccaaatcaga cagggcaaag aggtttttct tggtagttcg ctggcgcaag aggtcattga
    53041 cctcatccct actcttgtcc atctgctcaa tgacaggata gtaggcattg ctgatgattt
    53101 ccagactggc aaagagaaac ttataaatcg aaagcgtgtc atggttctcc agataacgag
    53161 tcatctgttc aatgacatag gcgttcttgg tattactaat ggtaatcagg cgacgatgct
    53221 cgacgataaa ggtcatggga aaagtctcat agtaggcctt gtcctttttt acgtctagga
    53281 cattatagat aaaggtaacc gtcccacttt cacggtggta gtccatgtgg gcgcgctcgt
    53341 ttctatccag tgcgtattca atggtttcct tgtccaaacc gtaaatttca gaaaggtctt
    53401 ctagtttatt caactttcct aggtctaggt ctatccaggt acaaccattg cccaactgct
    53461 tttctaaaac catcttttct ccttattaaa ctccctctat tgtaccacaa attacttaaa
    53521 ataacaggtc tagtctgtgc gtgagaaatt gctaacaact gtgatatttc ggttagggac
    53581 aaagtgaagg gcttgctcct cgctacgaag ctgtgtcgta cgaattccca cactgacaac
    53641 cttacccgat acagtaatcg gtccatttgt cagaacgacc tcatctccca catccagttg
    53701 acgttcaaag aggataaaaa agccattaat gacatcagac agaaagcctt gggctcccat
    53761 accaatcgct accccagcaa taccagctcc agccagcaaa ctagaaactg gcaaacctaa
    53821 aatcgacaaa atgcagtaga gtaaaaagaa atatagcgta taattaaaca cattttctag
    53881 taaacgtgag atggtttttt ggcgtccgac atcatgacga gacattttta gagaaggttt
    53941 gacaattctc tgcaccatgg tatgaagcat ttttttagca atataaaata caatcaaaag
    54001 cagtaaaaga gaaatgacct tggttagaat attctcgata atgcttgtca catctagctt
    54061 ttcaatataa gcctgaataa atttttgcat ataaaactcc tttatttcat tataccatat
    54121 tttcatcagt ctcattctcc ataaatattc aaaaaataga cagaaaattc ttgattttgt
    54181 ctatcattta tactataatc ataatcacta cacaaaaagg agattattat gaaaagaatc
    54241 attcatgctt ggaataaggc aagccttgtc aagcgtatct tgattggtat gctttgtggg
    54301 gaatcctaga actgaccctt cctaatctct cagggattgg gctactcggg gatttattcg
    54361 ttggaggtct aaaagctgtt gctcctatcc tagtctttgc cctcgttgcc aatgcccttt
    54421 cccaacatca aaagggacaa gatagcaata tgaaaactgt tgttttcttg tatatcctat
    54481 gatagagact ttcgacgctg ctcttgtagc tgtactagca agtttcatcg tccctattga
    54541 aattacccta aatagtgcta atactgaaat tgcaccacca gatgggattg ggcaggtcct
    54601 cagcaacctc ttgctcaaac tggttgacag cccagtcaac gccctgctta ctgctaacta
    54661 tattggaatc ttatcttggg cagtcatttt tggaatcgct atgagagaag ccagtaaaac
    54721 cagtaaaaat agtaaagaat tgctaaaaac tatcgctgac gtgacttcta aaattgtcga
    54781 atggatcatc aatctggctc catttggaat ccttggtctt gtttttaaaa ccatttctga
    54841 caagggagtc ggaagccttg ccaactacgg tattttattg gttccattag taacgactat
    54901 gctttttgtt gcccctgtgg tcaacccttt gattgccttc ttctttatga gacgcaatcc
    54961 ttactctcta gtttggaact gcctccgtgt cagcggtgtg acagcctttt tcactcgtag
    55021 ttctgcgact aacattcctg tcaacatgaa actctgccat gaccttggac tcaacccaga
    55081 tacctattct gtttctatcc cactcggttc tactatcaat atggctggag tagcgattac
    55141 cattaacgtt ttgacccttg ttacagttaa cactcttgga attcctgttg actttgccac
    55201 agcctttgtc ctcagtgtgg tagcagctat ctcagcctgt ggtgcttcag gtattgccgg
    55261 aggttccctc cttcttatcc cagttgcttg tagccttttc ggtatttcta acgatattgc
    55321 catacaagtt gttggggttg gttttgtgat tggtgtcatc caagactcat gtgaaacagc
    55381 ccttaactct tctacagatg tcctctttac cgccgttgcc gaatacgcag caacccgtaa
    55441 aaaataactc atcaaggcaa gcctgctcat gtcttgtctt ttacgctttt attctaactt
    55501 attaggaaat tcttatgtct attagccaac gtacgaccaa gctcatctta gctacctgtc
    55561 ttgcctgcct gcttgcttat tttctcaatc tttcgtcagc agtttcggct ggaattatcg
    55621 ctctcttgag cctatctgat acgcgtagaa gtactttaaa actggctcgc aatcgtcttt
    55681 tttctatgct tctagctctg gctatcggtg ttctagcttt tcacttgagc ggatttcata
    55741 tctggagtct cggcctctat ctggccttct acgttccttt agcctacaag atgggctggg
    55801 aaattggcat cacaccaagc actgttttgg ttagccatct cttggttcaa gagtcaacct
    55861 ctccagacct tctagtcaat gaattccttc tctttgctat tggtacagga tttgccttgc
    55921 ttgttaatct ctatatgcct tcacgagaag agaaaatcca gcactaccac acgctggtgg
    55981 aagaaaagtt aaaagatatc ctccagcgct tcaaatacta tttatccaga ggagacggac
    56041 gcaaccgagc acagctggta gcagaattag acacgctttt gaaagaagcc ctcagactgg
    56101 tctatttgga tcactctgac cacctctttc accagaccga ctaccatatc cactactttg
    56161 agatgagaca gcgacaaagt cgtatcctga gaaacatggc ccaacagatt aacacttgtc
    56221 accttgccgc cagtgaaagc ctgatcttag cgcaactctt ttcaaaaatt gcaggtcaac
    56281 tgagccagac caatcctgct tctgatttgc tagatgaaat tgaacgttat ctggaagtct
    56341 tccggaaccg cagtctgccc aagacaagag aagagtttga aacccgcgcc actcttcttc
    56401 aactcctacg tgaagccaaa accttcatcc aagtaaaagt tgatttttac caaaaatatg
    56461 gacagtaaaa aagaaaacca ctaaaaaaca gagcttgcca gcattcttga aaagtagtat
    56521 aattattgca tgcgcaatca tcttgtgacg cagagactgc ccgttaatgg actttcttct
    56581 atattatgac tctaaaaaga aaggagacac tatgacctat ttggaaaaat ggtttgactt
    56641 caatcggcgt cagaaagaaa ttgaaagtct cttggaagag accattgccc aacagagcga
    56701 gcaaagtctg accttgaaag agttctacct gctctactat ctggacttgg ctgaagaaaa
    56761 atctctacgc cagattgacc tgccagataa acttcatctg agcccgagcg cagtttctag
    56821 gatggtggct cgcttggaag ctaaaaattg cggtctactc agtcgcatgt gttgccatca
    56881 agatagacgc tctagtttta tctgcctgac aaatgatggg caaaagacac tggcctcgct
    56941 acaaaaaact gtcgaagaaa gcttggaagc aggtttggat ttcttgattt aagatgattt
    57001 ttaaaaaaag ttgcgtgcgc aatcattttt cttgacattc tcttttataa ggagtaaaat
    57061 aaagttagca ttaaacaaag gagttttaaa tatgattgaa attacctata tagatgccag
    57121 caagaacgaa agaactgtga ctttcgagtc ttatgaagac tttgaacgtt cgcaacaagc
    57181 ttgccttatc ggcgtcgcag actactaccc tgtccaaaaa ttaacttaca agggtcataa
    57241 tttggactac catgggactt atggagatat cttcttctat ctcatgaaac aagatttaag
    57301 ccaatataac taaaaaaagg agaaatacaa tggcaaaagc aattacagat gcaacattcg
    57361 aacaagaaac aaaagacggt ttggtcttag tagacttctg ggcaacttgg tgtggtccat
    57421 gtcgtatgca aggtccaatc ttggacaaat tgtctgaaga actttcagaa gatgtcttga
    57481 aaatcgttaa aatggacgtt gatgaaaatc caaacacagc tcgtgctttt ggaatcatgt
    57541 ctattccaac tcttctcttc aaaaaagacg gccaagttgt caaacaagtt gcaggtgttc
    57601 acacagcaga acaaatcaag gccatcattg ctgaattgag ctaatcaaac gagagaccaa
    57661 gtactttctt tggtctcttt tttcttgccc tttgctattt ttcaaaaaat atgctagact
    57721 atagtatatt gaaactagaa tagtacgcct ctacttctaa aacattgtta gaaatcgatt
    57781 tgactgtcct gatcgatttg tcctgttctt atttcatttt actatataag tagaatatta
    57841 cgatgtttgg gatatgccat cgctaaaaaa acctcgattt ggtatttttt agctccctaa
    57901 tgggagcttt ttgcgtgctc tgaacatttc ccattttgga aggagtacta tgaaacgtca
    57961 atcagccttg gtcgtcttta gtggtggtca agattcaaca acctgccttt tctgggccat
    58021 gcaacactat gaaacagtcg aagctgtcac ctttgcctac ggccaacgtc atcacctcga
    58081 aattcaaatt gctagagaaa tcgctaagga acagggcatt cgtcaccata tcctcgatat
    58141 gtctctgctg ggacaaatca ctgctcagcc agactttgcg acgattcata tttcctacat
    58201 tcctgacaag ctctgtgtcg agtcaaaacc cctcaaacta tatctattta gctaccgaaa
    58261 ccacggagat ttccacgaaa actgtatcaa caccatcggg aaagacttgg tcaacttgct
    58321 agaccctcgc tatttagaag tctggggaaa attcactccg cgcggtggca tttcaatcga
    58381 cccctactat aactacggta agcaaggaac taagtatgag ggcttggcag aacaacgcct
    58441 cttccaacac gacctttatc cagagaaaat tgacaaccgc taaactcata ctcaatgaaa
    58501 atcaaagagc aaactaggaa gttagccgca ggttgctcaa agcagtgttt tgaggttgta
    58561 gatgaaactg acgaagtcag taaccatacc tacggcaagg tgaagctgac gtggtttgaa
    58621 gagattttcg aagagtatca tacgaaaaag ccctgttcct caagatgagg agcaaggctt
    58681 tttgagtttc aattattctt ctgttccaag gaaatttttt gcaacaaggg ctgcaagaac
    58741 tccaccagcg attggtgcaa ggatgaaaat ccaaacttgt tgaagggctg cgccgcctac
    58801 caagacagct ggtgccaagc tacgagctgg gtttactgaa agtccagtaa tcttcaatcc
    58861 gacaagaatc atcgccatca atgacaaacc gattaccaaa ccagcaatcg cgccattgcc
    58921 cttgctttct gaagtcacag tcatgataac caagacaaac aagaaagttg cgatgacttc
    58981 aaacaagaaa ccaccaaaga cagtgacacc gtttgccaag gcattttcac caaggctagc
    59041 agttgacata cctgaattac ccaagaggaa gaagactgcg ccagaagcga tgaaagctcc
    59101 aacaacctga ccaaggatgt agtttacaag ttctgaagat gacaaacgtt tgtttacaaa
    59161 catagcaatc gaaacagccg ggttcaagtg agcacctgaa acagttccga ttgagtaggc
    59221 tgccaccacg attgccaaac caaaggcaaa ggcgattcca aggtgaccaa ggccatcaaa
    59281 accatttcca aaaacaacag ctcctgtccc aacgaacaca agcatgaacg taccgattaa
    59341 ctcagcaaca aattttttca ttttctttct ccttttttca aaaactagat actagtctat
    59401 caaaagtagg aaagggtttc aagaaaattg attggaaatt ttttgaaaat catagaacta
    59461 ttagctaatc cctagtattg aaaagactgg atagcttctt tcaggtcatc ttgtaaacta
    59521 tttctctggt caagttggac atagacttcc accagacagg atctaaagtt ggaaaatttg
    59581 taaaaatcct ccctttcttc tatcggaaaa tcaacagttt ttatccaaga agctacttgt
    59641 tcttgctcca acttcccttg taaaataggt tcatagatca ctcttgctaa acgccaatcc
    59701 tcatcatctg taaagcgaat cgacattctt ttaaatagtt ggccaagtat gtcaaatact
    59761 tcatgaattc tgtttatagg aaagtctgga tgacagacca cctctgtcag taaatcggct
    59821 ccatgtgcaa aagaatgaat ccaaccatac tgacttgaga aacctgttgt atccttttct
    59881 tttgaaagat agtgcaagcc ttgatttgaa aagacattac gaatttctaa ttgtaatccc
    59941 tgataaaaaa ccgattgctg gttggcatct gcatacaaga gatttgcata aacaagtgcc
    60001 ctaaaagaac gttcaagtgt tggcagacct accttatcaa tctctttatc tagtcctaca
    60061 tcagatgaaa ccccttcagc aatgaaatga aattgctcct gtgtaaatag ctcttcctga
    60121 atccctctag caaagcttgt aaaaacaagg tcatcgcgaa tttctggaga aggatctccc
    60181 aaatgatcaa gcaaccactg gatttcctcc tggttataat ttggtttttc ttctgctatt
    60241 tttcttagta aatcttgata catggtcaat acctctacat ttctagcaac tgttcaaaaa
    60301 agcagtctta aatgacctta ccaatttgta aaattaagtt aaaaagaaaa agacatttat
    60361 agtcagagaa acagcatatt tctgaaaaga tatataaatg atttattcca tattacaaca
    60421 aataaacttg taatgataga gccttatcac aaaaatcaca ttcctgtctc aaaaattgcg
    60481 aaaaactagt tataataaag tgaaataaaa actatacaaa ttgattaggg aatttaaaac
    60541 aatttctaac aatattttaa aaactgaacc gtactatttc aacttccata aattacaata
    60601 ttgataggca cagttcccat acctgcccaa attcgaataa cgtttagtat aaattagaat
    60661 atgactacaa catcacaaat aattagacta tctataatat ctacgatgta acatatacat
    60721 aggaagcacc gttatcaaga gcgccaaggc tatcaaccag ataagatgaa gatacaaacc
    60781 tgtcacctca cctccccata taatacgaat catgcgagta gcatgataca agggagaaaa
    60841 ccaagtcaaa tcaccaagta ttgggggcaa ggaagtaatg ggaaagaagg tatctgaaaa
    60901 catataaaga ggacggatta ccaaaacatt gtagtaattc agaaattccc tagacggcgc
    60961 caagtaggta aagattagtc ccaaaacacc aaacaaataa cccactataa ataaaatcag
    61021 aggaatcaat aacatcaaga catgaaaatg cgctgtgata agcatagaaa tcagccaaaa
    61081 aatcccaccg aaaatcactg cacgaatacc tgcccagata gcctgaccca ccacaatatc
    61141 actcgtattg attggcgtca tcaatatagc atcataagtc ttttcaatct tatactggat
    61201 aaaggcattg accgttgttt ctgcggtggc cgcagacata gcagtcgcag caattaaacc
    61261 tgtaatgaca aaatcacggt agagcatacc gttaacatgc gtcaaatagg caccaactcc
    61321 aaatcccatt gctaaaagat aaaagagggg gtcgataagg ttgggaacaa ttgaaatttt
    61381 aaataaattt ctaaaactca tgtagttgcg taccacaacg tgtccgatgg agtgaaatga
    61441 taacttaaac atgcaagtcc tccttctctg tcccagtcaa ccccaaaaaa agcgtctcaa
    61501 ggttgtcagc atcatacttg atgagcaagt cttctacagt tccttcttct ttgatagtcc
    61561 cttggtggag catcagcacc ctatctgtca aatattcaat ctcattcata tagtgggaag
    61621 taataatgat ggaaacacct aattccttca actccaaaag tttatcccaa ataatattgc
    61681 gggattgaat gtctaaacca actgtcggtt catctaaaat gataatatca ggccgattga
    61741 tgagagctct tgccaagaca agacgtcgtc gcatcccacc cgacagctga ttcaccatct
    61801 tttgcgcatg tttgctcagt tgaacaaaat ccaagagaga atcgattctc ttcctaagtt
    61861 cgacagatga gataccatag catagaccgt gagcaaaaag attatcataa acattcaagg
    61921 tcatgtccag catatcctcc tgccctacaa tacccagaca agatttactc ttacgacgat
    61981 tggtttcaat attttgcccc aagatatcaa tctctcctga ggaaattgag gtcagtgagt
    62041 aaatcatctt taaagtggat gttttgccag caccgttgat tcccaagata cccaaacact
    62101 ctccttctcc caattcaaag ctaattccct tgacagcttc ataagaacca tattgctttt
    62161 ttagattttt aacacttaac ttcttcactc tttctttccc ccacaaatag agcatgttgg
    62221 atactttgtt aattcatctc acttttgaaa aaatgttgag ggacgttaat cttaagatta
    62281 acgtccctca actgtggata ctgctgcaat tacttttgta attttacctc catttaaact
    62341 cagacaataa aatttaaaat attctctagt aaaacatttt taaaatctat tt
//
LOCUS       DBERCA010000007        58023 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.007, whole genome shotgun sequence.
ACCESSION   DBERCA010000007 DBERCA010000000
VERSION     DBERCA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 58023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.007"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(224..586)
                     /locus_tag="ACL719_000488"
     CDS             complement(224..586)
                     /locus_tag="ACL719_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGS0698511.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            complement(856..1014)
                     /locus_tag="ACL719_000489"
                     /pseudo
     CDS             complement(856..1014)
                     /locus_tag="ACL719_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1310..2616
                     /locus_tag="ACL719_000490"
                     /pseudo
     CDS             1310..2616
                     /locus_tag="ACL719_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2705..3394)
                     /gene="rplA"
                     /locus_tag="ACL719_000491"
     CDS             complement(2705..3394)
                     /gene="rplA"
                     /locus_tag="ACL719_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGS0698512.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(3602..4027)
                     /gene="rplK"
                     /locus_tag="ACL719_000492"
     CDS             complement(3602..4027)
                     /gene="rplK"
                     /locus_tag="ACL719_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGS0698513.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(4336..5052)
                     /gene="ldcB"
                     /locus_tag="ACL719_000493"
     CDS             complement(4336..5052)
                     /gene="ldcB"
                     /locus_tag="ACL719_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGS0698514.1"
                     /translation="MKKRYIVLSGLLAVTLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(5078..5539)
                     /locus_tag="ACL719_000494"
     CDS             complement(5078..5539)
                     /locus_tag="ACL719_000494"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS0698515.1"
                     /translation="MCLICQRIEWIKAGENPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KEHVTELHHMETSVKLRFLEEMSLVQEAVAKTFKAEKMNIELLGNGDAHAHWHLFPRR
                     SGDMRGHGLNGRGPVWWVPWEEMAAEDCQVKSHELEQMIKALSDELEKYTV"
     gene            complement(5541..6578)
                     /locus_tag="ACL719_000495"
     CDS             complement(5541..6578)
                     /locus_tag="ACL719_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGS0698516.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIELPVTTHFAFSVFEE
                     VGHGANSNIPSQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVVLA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(6676..8001)
                     /gene="brnQ"
                     /locus_tag="ACL719_000496"
     CDS             complement(6676..8001)
                     /gene="brnQ"
                     /locus_tag="ACL719_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGS0698517.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(8172..8459)
                     /locus_tag="ACL719_000497"
                     /pseudo
     CDS             complement(8172..8459)
                     /locus_tag="ACL719_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(8619..9197)
                     /locus_tag="ACL719_000498"
     CDS             complement(8619..9197)
                     /locus_tag="ACL719_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGS0698518.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFLLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(9251..10651)
                     /gene="pepV"
                     /locus_tag="ACL719_000499"
     CDS             complement(9251..10651)
                     /gene="pepV"
                     /locus_tag="ACL719_000499"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGS0698519.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(10664..11269)
                     /locus_tag="ACL719_000500"
     CDS             complement(10664..11269)
                     /locus_tag="ACL719_000500"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGS0698520.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIADRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            11440..12240
                     /locus_tag="ACL719_000501"
     CDS             11440..12240
                     /locus_tag="ACL719_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698521.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(12422..13219)
                     /locus_tag="ACL719_000502"
     CDS             complement(12422..13219)
                     /locus_tag="ACL719_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0698522.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNVIEVEDGELVNILFRKVAYDYEAELEFAK
                     SKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKNFFDFL"
     gene            complement(13209..15056)
                     /gene="uvrC"
                     /locus_tag="ACL719_000503"
     CDS             complement(13209..15056)
                     /gene="uvrC"
                     /locus_tag="ACL719_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGS0698523.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     GVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNEP"
     gene            complement(15095..15796)
                     /locus_tag="ACL719_000504"
     CDS             complement(15095..15796)
                     /locus_tag="ACL719_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGS0698524.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(15875..17107)
                     /gene="murN"
                     /locus_tag="ACL719_000505"
     CDS             complement(15875..17107)
                     /gene="murN"
                     /locus_tag="ACL719_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGS0698525.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(17112..18332)
                     /gene="murM"
                     /locus_tag="ACL719_000506"
     CDS             complement(17112..18332)
                     /gene="murM"
                     /locus_tag="ACL719_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGS0698526.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQTIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(18444..19223)
                     /locus_tag="ACL719_000507"
     CDS             complement(18444..19223)
                     /locus_tag="ACL719_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0698527.1"
                     /translation="MAVMKIEYYSQVLDMEWGMNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(19292..20953)
                     /locus_tag="ACL719_000508"
     CDS             complement(19292..20953)
                     /locus_tag="ACL719_000508"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS0698528.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(21128..23350)
                     /gene="recJ"
                     /locus_tag="ACL719_000509"
     CDS             complement(21128..23350)
                     /gene="recJ"
                     /locus_tag="ACL719_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGS0698529.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIAFMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGMVQEIYDFLMEKE
                     "
     gene            23627..24385
                     /locus_tag="ACL719_000510"
     CDS             23627..24385
                     /locus_tag="ACL719_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0698530.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            24396..25190
                     /locus_tag="ACL719_000511"
     CDS             24396..25190
                     /locus_tag="ACL719_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS0698531.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            25203..25880
                     /locus_tag="ACL719_000512"
     CDS             25203..25880
                     /locus_tag="ACL719_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0698532.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            25890..26549
                     /locus_tag="ACL719_000513"
     CDS             25890..26549
                     /locus_tag="ACL719_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0698533.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            26807..27997
                     /locus_tag="ACL719_000514"
     CDS             26807..27997
                     /locus_tag="ACL719_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGS0698534.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTIKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(28318..29199)
                     /locus_tag="ACL719_000515"
     CDS             complement(28318..29199)
                     /locus_tag="ACL719_000515"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGS0698535.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(29340..30668)
                     /gene="vncS"
                     /locus_tag="ACL719_000516"
     CDS             complement(29340..30668)
                     /gene="vncS"
                     /locus_tag="ACL719_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGS0698536.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMYLDAQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(30665..31321)
                     /gene="vncR"
                     /locus_tag="ACL719_000517"
     CDS             complement(30665..31321)
                     /gene="vncR"
                     /locus_tag="ACL719_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGS0698537.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(31370..31453)
                     /gene="pep27"
                     /locus_tag="ACL719_000518"
     CDS             complement(31370..31453)
                     /gene="pep27"
                     /locus_tag="ACL719_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGS0698538.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(31633..33012)
                     /gene="vex3"
                     /locus_tag="ACL719_000519"
     CDS             complement(31633..33012)
                     /gene="vex3"
                     /locus_tag="ACL719_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGS0698539.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQVSILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(33064..33711)
                     /gene="vex2"
                     /locus_tag="ACL719_000520"
     CDS             complement(33064..33711)
                     /gene="vex2"
                     /locus_tag="ACL719_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGS0698540.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(33724..35001)
                     /locus_tag="ACL719_000521"
     CDS             complement(33724..35001)
                     /locus_tag="ACL719_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698541.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(35358..35528)
                     /locus_tag="ACL719_000522"
                     /pseudo
     CDS             complement(35358..35528)
                     /locus_tag="ACL719_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(35671..36270)
                     /locus_tag="ACL719_000523"
     CDS             complement(35671..36270)
                     /locus_tag="ACL719_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698542.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYAPLNDVTKYAR"
     gene            complement(36512..37396)
                     /locus_tag="ACL719_000524"
     CDS             complement(36512..37396)
                     /locus_tag="ACL719_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0698543.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(37400..37786)
                     /locus_tag="ACL719_000525"
     CDS             complement(37400..37786)
                     /locus_tag="ACL719_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGS0698544.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(37779..39122)
                     /gene="cysS"
                     /locus_tag="ACL719_000526"
     CDS             complement(37779..39122)
                     /gene="cysS"
                     /locus_tag="ACL719_000526"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGS0698545.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMTKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLAAQGIKLLDTKDGVRWTRD"
     gene            complement(39204..40088)
                     /locus_tag="ACL719_000527"
     CDS             complement(39204..40088)
                     /locus_tag="ACL719_000527"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698546.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFEREIASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(40100..40717)
                     /gene="cysE"
                     /locus_tag="ACL719_000528"
     CDS             complement(40100..40717)
                     /gene="cysE"
                     /locus_tag="ACL719_000528"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGS0698547.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(40733..42946)
                     /gene="pnp"
                     /locus_tag="ACL719_000529"
     CDS             complement(40733..42946)
                     /gene="pnp"
                     /locus_tag="ACL719_000529"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGS0698548.1"
                     /translation="MAKQVFQTTFVGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELITFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(43631..44497)
                     /gene="metF"
                     /locus_tag="ACL719_000530"
     CDS             complement(43631..44497)
                     /gene="metF"
                     /locus_tag="ACL719_000530"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGS0698549.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(44561..46810)
                     /gene="metE"
                     /locus_tag="ACL719_000531"
     CDS             complement(44561..46810)
                     /gene="metE"
                     /locus_tag="ACL719_000531"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGS0698550.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(47071..47337)
                     /locus_tag="ACL719_000532"
     CDS             complement(47071..47337)
                     /locus_tag="ACL719_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0698551.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(47463..47910)
                     /locus_tag="ACL719_000533"
                     /pseudo
     CDS             complement(47463..47910)
                     /locus_tag="ACL719_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            48066..49067
                     /locus_tag="ACL719_000534"
     CDS             48066..49067
                     /locus_tag="ACL719_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS0698552.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(49135..51540)
                     /gene="pheT"
                     /locus_tag="ACL719_000535"
     CDS             complement(49135..51540)
                     /gene="pheT"
                     /locus_tag="ACL719_000535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGS0698553.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDK
                     APYYAARILENVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAASSLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(51617..52126)
                     /locus_tag="ACL719_000536"
     CDS             complement(51617..52126)
                     /locus_tag="ACL719_000536"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698554.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GIGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(52126..53172)
                     /gene="pheS"
                     /locus_tag="ACL719_000537"
     CDS             complement(52126..53172)
                     /gene="pheS"
                     /locus_tag="ACL719_000537"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGS0698555.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(53760..55175)
                     /locus_tag="ACL719_000538"
     CDS             complement(53760..55175)
                     /locus_tag="ACL719_000538"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS0698556.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(55188..57026)
                     /locus_tag="ACL719_000539"
     CDS             complement(55188..57026)
                     /locus_tag="ACL719_000539"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGS0698557.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAASFSALLAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGIELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(57044..57883)
                     /gene="licT"
                     /locus_tag="ACL719_000540"
     CDS             complement(57044..57883)
                     /gene="licT"
                     /locus_tag="ACL719_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGS0698558.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFVALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT        16671 a        13023 c        10614 g        17715 t
ORIGIN      
        1 ataaactaac ctcctcatta ttagcaaggc ttctactgat tataacctaa ttcttagact
       61 acttcaagac taactgtcca ataataatct acagataaat catccactct taatcaaatt
      121 ttattcaatc catctactca ttcccaaaag gttgctgtat aatgcaagac atttttctta
      181 ctgcttttac ttttccttac cctcaattta gtgagctaat aaattattct tcttcaaaag
      241 gttgagattt atctagtaac tctaaaacat taatggtagg tagcaacatt gcttcactgc
      301 tatcaattgc cgaaactatt gaaacaatag aacgtaaata aggataaaga atagcagtac
      361 catttacgta tagaatatcg gtgatatttt catctatatt tatttcaaaa tatcctatta
      421 cagttacaga tatttttttc atggcatctt tgtcaactaa agaaacacta agttttacgg
      481 ctccatgact ataatcatct gtatgaccaa ccttaactat gggatataat atatcgaagg
      541 taaatgtgaa caaactgatt agggaattta aaccaacttc taacaatgtg ttatactcaa
      601 tgaaaaccaa agagcagact aggaaactag cctcaggttg ctcaaaacac tgttttgagg
      661 ttgtggatag aactgacgaa gtcagtaacc atacgcacgg caaggcgacg ttgacgtggt
      721 ttgaagagat tttcaaagag tattagaaag ctcgatggac tattccagat tcattccact
      781 agatttcgaa atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtgccgt
      841 tcggcatacg gcagttcaac taacttttaa cgcatgtcgt tcaaggtaat aatccaaaca
      901 cgaaaccagt tcacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt
      961 ctgagctact aattgatagt ggtcgcccca gccagatacc ttatccgcta tataggcctt
     1021 attatcacgt tcctgttcgt agagccacga tttcgctatc ccttcctctg acgattctta
     1081 tttcctgcgt cagacttggg aatcgctatt gggttcaccg gtagcaggtg cccactgagg
     1141 acttacacct cagatatacg acatgcccgt cgtacatcaa aaaaagggct ctataatatt
     1201 tgtagtgggt aaccccccta tagatattat ggagcctatt ttgtgtagaa aaaaagtccc
     1261 atatgaccta taatgaaaag cgacaaaaca actcattaga aagattcata tggaacaatt
     1321 acattttatc acaaaattac tagacattaa agaccctaat atccagattt tagacatcat
     1381 caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc catcttgccc
     1441 tgagtgcgga aaccaattga agaaatatga ctttcaaaga ccttctaaaa ttccttatct
     1501 tgaaacgact ggtatgccta ctagaattct ccttagaaaa cgccgtttca agtgctatca
     1561 ctgttcaaaa atgatggttg ctgagactcc cctggtaaag aaaaatcacc aaatccctcg
     1621 tatcatcaac caaaagattg ctcaaaagtt aattgaaaag atttctatga ctgatattgc
     1681 ccatcagctt tccatctcaa cttcaactgt tattcgtaag ctcaatgatt ttcactttaa
     1741 acatgatttt tcttgtcttc ctgagattat gtcttgggat gagtatgctt ttacaaaggg
     1801 aaagatgagc ttcattgcgc aagattttga taatcttaat attatcacta ttcttaaagg
     1861 cagaacacaa gctgtcatcc gaaatcactt tcttcgctac gatagagctg tccgatgtcg
     1921 cgtcaaaatc attactatgg atatgtttag cctttactat gacttggcta aacagcttcg
     1981 ctttcaaatt tctaggctca ggctgaaaca gtctcccaga ctgtttcact cccgaatgct
     2041 aaaatcgttc ttgaacgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg
     2101 cgtgtccaaa tcatgaatca gtttcatcga aaatcccatg aatacaaggc tattaagcgc
     2161 tactggaaac tcattcaaca ggatagccgt aaactgagtg ataagcgatt ttatcgtcct
     2221 acttttcgta tgcacttaac caataaagag attttaaaca agcttttgag ctattcggaa
     2281 gacttgaaac accactatca gctctatcag ctcttgcttt ttcacttcca gaataaggaa
     2341 ccagagaaat ttttcggact tattgaggac aatcttaagc aggctcatcc tatttttcag
     2401 actgtcttta aaaccttcct caaagataaa gaaaagattg tcaacgccct tcaactacac
     2461 tattctaatg ccaaactgga agcgaccaat aatctcatca aacttatcaa gcgcaatgcc
     2521 tttggttttc gaaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa
     2581 aaagaaagga caaaatttgt cctttctcga gcttagcttt ttttcaaccc actacagttg
     2641 acaaagagcc aaaaaaagtt gagacatagc gtcttcaact tttttacatt actttaacta
     2701 ctgattaaag tgagtttaca tcaactttga taccaacacc ttgagtagtt gtgatagtca
     2761 agtttgttac gtaagttcct ttagctgtag ctggttttgc tttttggatt gtttcgttga
     2821 aagctttaaa gttttcaacc aatttttcag cttcaaatga tactttaccg atgattgctt
     2881 gaacgttacc tgcacggtca gcacggtaag tgattttacc acctttagac tcttcaactg
     2941 ctttagcaac atccattgtt acagtaccag ttttagggtt tggcatcaag ttacgtggtc
     3001 caaggacacg tccaagacgt ccaacaagag ccatcatatc aggtgtagcg ataactacgt
     3061 cgaagtccaa ccaaccgtcg ttgattttag caacaaggtc atcttcacca acaaagtctg
     3121 caccagcagc ttttgcttct tcagcttttg caccacgtgc gaaaacaaga acacgtgaag
     3181 ttttaccagt accgtttggc aataccattg ctccacggat ttgttggtca gcttttttaa
     3241 cgtcgatgtt caagttgtaa gcaacttcta cagttgcgtc aaattttgca aagttagttt
     3301 cttttgcaag tgctacagct tcttctacgc tgtatgcttt tgtgctgtcg attttctcaa
     3361 gagcagcacg aagttgtttg ctttttttag ccattttcta ttctccttgt aagtggttca
     3421 atcgattttc atctcccacg tcacttctcg aaatgatgaa gtcttgcggg ttttcagtct
     3481 actatttttc tcttcctacg gccttaagct cccttgctgt acccaagtac aagcctcagt
     3541 cacttgccta gtatgaaaag caactaatag actgctgtga gattttctac tgtgttattg
     3601 attagtcaac aacagtgaat cccatagaac gagcagtacc ttcgatcata cgcattgcag
     3661 actctacgtt tgctgcgttc aaatctggca tcttagtttc tgcaatttct tgtacttgtg
     3721 cacgagtaac tgtagcaact ttagttttat taggtgtacc tgatcctttt tcaacacctg
     3781 cagctttttt caaaagaaca gcagctggtg gtgttttcgt aacgaaagta aatgatttat
     3841 cttcgtaaac tgagataaca actggaatga tcataccagc ttggtcagct gtacgagcgt
     3901 tgaactcttt tgtgaatccc atgatgttga taccagcttg accaagagca ggtccaaccg
     3961 gtggagctgg tgtagcttta ccagcaggga tttgcaattt tacaagtttt tcgacttttt
     4021 tagccatttt taaatcctcc tttgtggttt tggcggtaat tagagatttt tacctcccac
     4081 aagtatgttt ttcgcatacc cattcattat acactattta tctggaattg caagagaaaa
     4141 gtttattttt tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc
     4201 tcaaagtaca gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt
     4261 gaggttgtga atagaaccga cgcagtcagt aggtaagacg acgctgacgt ggtttgaaga
     4321 gattttcgaa gagtattaat cgacgtagtc tccgccttca aagccatagt attcttccaa
     4381 actgagacca cttgcagcaa tttctttagc ttcttttcct acataacgca ggtgccattc
     4441 ctcagccata tagcctgttt ccttttcctt gcctttgaga taacggacaa caaagccata
     4501 atcagctgca tgatccaaga gccattgggc tgctttttct tctgtcacca aatcaccatc
     4561 agtcccaatc acatcaaagg ccaagcctgt ctggtgttcg ctatagccag gacgggcaga
     4621 gtaacggtca gctgctgcct ttccatcttg gttgacataa tcttgataga gcttggtctg
     4681 agtttcataa cttctaaaac cactgtaatg atcactaata gggaaacctg cctcttgcat
     4741 cgctttgatg agtttgacca actcagcctt ggctgttgga ttttccccgg gattatagtc
     4801 tttcgacaaa ggataatgtt tattggctac gatgatttcg tcgtattttc cttggatgct
     4861 gtagtaatca cctttattga ccacttctgc ttttttctgg gcagcccctt gagacttact
     4921 gccgactgtc ccatcagctt tggctgtctg ttctgtctta gctgtgccat cttcattttt
     4981 tgctttttct tgtgaacaag ctgctagggt tactgccagc aaaccagata aaacgatgta
     5041 tctttttttc attttttctt cctctattct tcctctctta gaccgtgtac ttctctaatt
     5101 catcggataa ggctttaatc atttgttcca actcatggga tttcacttgg caatcttctg
     5161 ccgccatttc ttcccagggc acccaccaga ctggaccacg gccattcaat ccatgacccc
     5221 tcatatcacc tgaccgtctt ggaaagagat gccagtgagc gtgggcatct ccatttccta
     5281 gaagttcaat gttcatcttt tcagctttaa acgttttggc aacagcttct tggaccaaac
     5341 tcatttcctc tagaaaacga agttttacag aagtctccat atgatggagt tctgtgacat
     5401 gctcttttgc tagaaataag gtatagccct taaagtattg gtggtctcca ataacaacat
     5461 agcctgtttc tagttctttt acaaagtagg gattttcccc tgccttgatc cattcaattc
     5521 tttgacaaat caagcacata ttagtctacc aatgcactct taagataagc atcgaccata
     5581 cgctcagtcg ccactactga atcaatgtgt gttcgttcgt aagagtgact tgactcaata
     5641 ccagctccaa ggagagcatg ctttacttct gctcctgcag acatagctgc cgaagcatcc
     5701 gaaccgtaaa atggataaat gtcgagctta aatggaatat cttgctcctt cgccaaaact
     5761 accaagtgtt gacggaagtc atagtgatag ggacctgaag cgtccttgac acagatagac
     5821 actgtatact catccgtctg ctggtcatct cccatagctc ccatatctac agccagatat
     5881 tctactacct gacttggaat attggagttt gcaccgtggc ccacctcttc gaagactgaa
     5941 aaagcaaaat gagttgttac tggcaattca atcccctctt ccttataaac acgaagaaga
     6001 ttgagcaaaa ttgctgcgct gaccttgtca tccaagtggc gagacttgat aaaaccagtc
     6061 tctgtcacga cagttcgtgg atcgaagctg ataaaatcac cgacctcaat cccaagagca
     6121 cgagtttctt tttcattgct tacttttaca tccaaacgca cttccatatt gtcctgcgtg
     6181 cgttctgcag ttcctgcatc cttgtagaca tggcaagaag tttggtggat gaggatggtt
     6241 cctgatacct tttgacctgt gctagccaca tgaacggtac agttttcacc ttcaatcatg
     6301 ttccaaggaa aaccaccgat acggtccaat ttaagacgac catctggttt gacagcacga
     6361 acaatagcac ccagcgtgtc cacatgggca gtcacatagc gatgttgctc atcattttga
     6421 cctttgatgg tcacattgac accacccttg gctgtgcgaa ctggctggta accaaaacct
     6481 tctagagtct tgactaaata attcgaaatt tcacgagtga agcccgttgg cgatgcgatg
     6541 gctgtcagtt ctttgatata ttctactgtt tgattcattt cttcacctct ttcgctacct
     6601 attataacac atttatcatc ggataaaaaa agaaaatcac tcctgtagac ttggctacaa
     6661 aagtgattta agtgattatt ccatttcaaa aacatcgctt tcttgcttgt ttggtagaac
     6721 caatgagagc aagattccaa caatggctgg caccaaccat gggagagatg ccttggcaaa
     6781 aggaagaacg ctaacaagat ttgcaagaaa ctcaacctta aacgagcttc ctagtacgct
     6841 tgcaatggca ataactgtaa ccacagcaat tgtcaactgc atacctggtt ttgaaagggc
     6901 aacaaatttg ttgacaatga caatcataac gatagcaatc gtgattgggt acaagataac
     6961 cagtactgga attgagtact tgataatcgc atcaagaccc aaattggcaa tagcaaatcc
     7021 aatcaaggta aaggctgtcg cataaacctt gtagctgatt tgtgggaagc gctcattaaa
     7081 gaactcagct gttgacacaa tcaaaccaac agtcgttgtg aagcaggtta cggtaaccat
     7141 agctgcaagg aagagttgag ctgttgagcc aaagatttct tgagtggctt gtgacaagat
     7201 gtaaacacct ggtgttccac ccttcatcgc ttcagctggt actgggaaat gatttccaag
     7261 aaaacctaaa ccgatgtaaa gagcgctgaa ggcaagggca acaacgatac caacaaccca
     7321 aatagttgaa atgtattctt tcttacttga aaatccaagt tgtttcaagg tttgaactgc
     7381 gattacgcta aaggccactg aggcaagggc gtccaaggta ttgtaacctt ctaggaaacc
     7441 tgtaccaaag gcagaagctt gataagcagc tgaagcagct tgaggacttg ttccaccata
     7501 tttgatagct cccagaacga ccaagataac aatcaaaatt gcaaagactg gcgttaaaat
     7561 acgtccaatg cggtctaaga tttttgatgg attaagcgaa atcaaatagg ctgccgcaaa
     7621 atacagaacc gtaaatacaa tcaagccaag tcctttattt gcatccgaca aaagggggct
     7681 aatccctact tcgtaagctg ttgtagcagt acgtggggta gcaaagaatg gaccgattga
     7741 caagtaaaga actgagaggt aaagagtcgc aaaccaaggc gctatcttcg ttgaaatctc
     7801 gtagatatat cctttaggat ttagcgttcc aataataagg gtcaagacgg cgataccaac
     7861 gcctgaaaag acaaaacctg cgatggcagg aagaaaatgt tctccagata gagcacctag
     7921 agaaggcgga aaaatcaagt tccccgcacc aaaaaatatt ccaaacagga gcaaacctgt
     7981 tagggtacct tttttagcca taaaaatctc cttcatattt ataaaatact gacctagtat
     8041 accacgatta ggagtctgaa aaaagcatca cgaggcaaat attaaatgtt ttcaattttc
     8101 ttaaaaataa gaattatcta aaaataaaac tctcctaccc agtccacacc aagtaggaga
     8161 aatgcaaaat tttaaagttc ttcaaaggct aacatgccac cctggacatt gataacgtta
     8221 taaccttgtt ctaacaggaa ttggcaagca cgcgccgatc tcattccaga tttgcaaata
     8281 atataatgca agcgatcttt gtccaattaa tcataactat cagccaattg actaagcggt
     8341 aggttgtggg taccttctaa atgaagagct gcaaactcat ccacttctct cacatccact
     8401 agagaaagct ggtcgttttg gtaaagctga taaaatgcgt caaaggctat ttctttcatt
     8461 ctttttcttc taatactctc cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgtg
     8521 cgtatgttac cgacttcgtc agttttatct gcaacctcaa agctgtactt tgagcagcct
     8581 acgactagct tcctagtttg ctctttgatt ttcattgact ataaaatggt tttaattctt
     8641 tttttcaaat ctggcactac ttctgcctca aaccaaggat ttttggccat ccagatttga
     8701 tttcgtggtg atgggtgaac tagcagaaaa taggctggca gatagtcttt atagtgtttc
     8761 accctctccg ttaccttccc actgattttc tcctgtaaat agtaggcttg ggcatattgc
     8821 ccaatcaaga gggttaactg aatatcaggc aattcctgta agagctgcgg atgccatttt
     8881 tctgcaaaac ctgtacgagg cggaagatca cccgacttgc catgtcctgg aaagtagaaa
     8941 tccataggca aaacagcaaa ataacctgaa ttgtaaaagg tatcttcatc tacacctagc
     9001 cagtcccgca agcggtcacc acttttatct ttccagtaaa ggcctgcttc ttgagtttta
     9061 agtcccggag cctgaccgat gatattgatg cgagcagttt ttggcgctgc aaagagaggc
     9121 tcaatgccac gctctgtata gctggcattc tgcgaatccg ccatgatagc ctgtttgatt
     9181 ctttcgattt gagacatcta ctttccctcc aaaaagaagt ccaagcataa gatctcagac
     9241 ttctatcgtt ttatttgatc aattcgtaaa tagcttcggc ataaattgct gctgctcgga
     9301 agagatcgtc caaggcaata aattcattgg cttggtgcat agtatcaatt gagtctggga
     9361 acatagcacc ataggcaact ccgcgctcta acaagcgacc aaaggttcca ccaccgatga
     9421 cttgctcatg accttgtagg ccagtttgtt tttcatagac attcaacaag gtttgcacaa
     9481 gtggatcttc cattggcaca tagtgagggg tatgaccatg ttctgataga ctgacagaag
     9541 caactggcaa gttttctagg attgacttga tttgttctgg acttgttcct tttggatagc
     9601 ggatgttaag ggcaatggta ttatcagcac ttgtttcatc gaagcggaag acgccggcat
     9661 tcatagaaag agcccccatc ttttcatcca catgagcaat cttgagattt tcaccctcat
     9721 gatcgttcaa gagaatttta cctgcgatgt caaggtagtc tttggctgga ccagcaaagc
     9781 caaactggct gaggaagagg gcaaggtaag ttgcgccatt gacacctgaa gcaggcatag
     9841 caccgtgggc tgatttacca atgatcgtca ccttgtattt gccagcttct tcttggagtt
     9901 ctcctctaag tttgtgttct gcaacaaagg catctagttt agcttgcaag tcagccaaat
     9961 cacctgaaac gactgctgtt gctgattctg gtaccatatt ttcacgtaaa ccacctgtaa
    10021 agctgtgaag acgggcagca cctgtatttt ctcctgcaaa gtggaggtat tccgtgatat
    10081 ttcctttttc accattgatg attggaaatt cagcatctgg tgagaaaccg aaatctggtt
    10141 tggcaagtcc tacgtgctca aagtagtagt ccatgtctgc ccagcctgat tcttcgtctg
    10201 ttccaacgat gaagcgaact ttcttagaag ttggaaggcc taattctttg atgattttca
    10261 aaccatagta gcaagctgtt gtaggtccct tgtcatctga agcaccacgc gcataaaggc
    10321 gaccatcttt gatagttggt gtgtaaggat ctgtatccca accgctacca gctggaacca
    10381 catccatatg ggcaaagatt ccgagaactt cttctccatc accaaactca aaatgtcctg
    10441 catagttatc aacattttta gttggataac catcacggtc tgcgatttca aggaattttt
    10501 ccaaggcttt tactggacca ggtccaaatg gatgttgggc atcagccttg ctatcatcac
    10561 gttctgagtt gatttccaaa aggctaaaca agtcagccaa gaggtcttct ttgcgttttt
    10621 ctacttctgc tgtaaaatca attgctatca ttttttcttc ttcctatctt ttttcgatga
    10681 tttcatctac tggcaagcgg tagcttggtt ccaatttgtc gtctgtgtaa cccactgtga
    10741 tcaagagttc tgggcggaaa cggtctgcga tttccaaaac ttcattaact tttgatttgt
    10801 caaaaccaag gataatattg gaaccaattc cttggtctgt aagagcgaga accaagttca
    10861 tagcaaccaa acctgcattg agtgctaggt agtcgctgac ttgttgttca ttgtagcgtg
    10921 caaattcagc tggcaaattt ttcatgaagt attgaagttg ttcttctgag aaattgttag
    10981 caccaccaac tcgggcaatc ttacgagcgc gtttggcaag gtctgtatct gtaaataggg
    11041 caatggttac aggtgctgat gatacctgtt caaaattgga accataagct aactttgcca
    11101 gttcagcatt tttctcacgt accaccacaa atttccaagg ctggctgttg tgggcgcttg
    11161 gcgccaaggt tgcaatttcg atagccgtac gcacatcttt gggatcaaca ggcttatcag
    11221 taaaatgctt ggtcgcatga cgttttttat ttaattcaag aaatttcata atcgatttcc
    11281 ttttctaatt ctactgcttc cattctacca taatttgaaa gagcttgcag ggatttttca
    11341 tgattaagtt tttctatcac agacataaaa gatatatatt ggtttatcag gaaagtttct
    11401 gataaactag caagtacttt acatttgaat ggagacagta tgaaaaaatt tagcctatta
    11461 ctagctatcc taccattttt ggttgcctgt gggaatcaag ctacacccaa agagactagc
    11521 gctcaaaaga caatcgtcct tgctacagct ggcgacgtgc caccatttga ctacgaagac
    11581 aagggcaatc tgacaggctt cgatatcgaa gttttaaagg cagtagatga aaaactcagc
    11641 gactacgaga ttcaattcca aagaaccgcc tgggagagca tcttcccagg acttgattct
    11701 ggtcactatc aggctgcggc caataacttg agttacacaa aagagcgtgc tgaaaaatac
    11761 ctttactcgc ttccaatttc caacaatccc ctcgtccttg tcagcaacaa gaaaaatcct
    11821 ttgacttctc ttgaccagat cgctggtaaa acaacacaag aggataccgg aacttctaac
    11881 gctcaattca tcaataactg gaatcagaaa cacactgata atcccgctac aattaatttt
    11941 tctggtgagg atattggtaa acgaatccta gaccttgcta acggagagtt tgatttccta
    12001 gtttttgaca aggtatccgt tcaaaagatt atcaaggacc gtggtttaga cctctcagtc
    12061 gttgatttac cttctgcaga tagccccagc aattatatca ttttctcaag cgaccaaaaa
    12121 gagtttaaag agcaatttga taaagcgctc aaagaactct atcaagacgg aacccttgaa
    12181 aaactcagca atacctatct aggtggttct tacctcccag atcaatctca gttacaataa
    12241 cactcttcga aaatctcttc aaaccacgtc agctttatct gcaacctcaa agctgtgctt
    12301 tgagcaacct acggctagct tcctagtttg ctctttgatt ttcattgagt ataagatata
    12361 ctaaaagcga tgggatcagc caatcgcttt tttagtttgc attggttatt ttaggaaact
    12421 cttacaaaaa atcaaaaaaa ttcttcacat cctctacata cccatgcttt tctattaagc
    12481 ttgctaatag ttccagattg tgcccctgat agttatcttc acgacgtagt tcttcataca
    12541 tttcgataaa gggaagcccc ttggatttgg caaattctaa ctcagcctcg taatcataag
    12601 cgactttacg aaataggata tttaccaatt ccccatcttc aacctctatc acgttaaaat
    12661 agggcatgcc aatcgaccct ggattgatga tttgttgccc ttgactacca taacgaagca
    12721 actgcttgtg aacatgacca taaactgcca cgtccgtttc cgcatctagc agttggtcaa
    12781 atttctctgt atcattctca actagcaagt caccaccata gtttttgtca ggtaaattat
    12841 gagagataga aaagcgcaat ccgtcaattt ctttcttttc cagcaaaggc aagcttcgta
    12901 gccagacaat cgttgcagga tccattcgct ccatcaaata ctgtgtcata cgcaagagct
    12961 gaacttcctg tgggtcttct aagccatatt gcccatctaa agcctcaagg acacgatcat
    13021 cccaattgcc tcgaacactt gctgtgatag gaaggtcctt tagcagggcg actaagtcat
    13081 ttgcgcctgg accaggaaga aaaatatctc ccagaagcca gtattcactg actccttgat
    13141 ttttagcatc tgcaatcact gcttctagcg ccgtcgcatt gccatgaata tctgataaaa
    13201 ttgcgatttt atggttcatt gtggtttcct tctgtttggt aatctactct ttcttccgcc
    13261 acttgaagca agatttctgt ttcctgcggg ttcaactttc tttgcacagc ctctgctact
    13321 gctcttggta ctccaacttc gacaatctca tccacaccgg cttccttgat tttggtcaga
    13381 gacttgaagt gcctcatgag attttgcttg cgtttaggtc ccagaccatc aatcccatcc
    13441 aattgagatg agaaagaatt tttggagcgc agttggcggt ggaaggtaat ggcaaagcgg
    13501 tgcacctcat cttggatgcg ttggaggagg aaaaattcct gagaattgcg agacaagtcc
    13561 accacctcaa gcggatctcc aaagagcagt tcatgggttt ggtgcttatc attcttttgc
    13621 agacctgcaa ttggaatatc caagcccagt tcttcttgga tgacttgctt agcaatattg
    13681 acttgacctt gccccccatc aatcacaatc aaatctggag gagtcaaagc ctcacgctgt
    13741 actcgaccat agcgtctgcg aatgacttct ctcatactag catagtcgtc cggcccaaca
    13801 acggttttta tcttgtactt acggtaatct tttttacttg gcctgccgtt aacaaagacc
    13861 accatggctg aaacaggact ggtccccatg atgttggagt tatcgaagga ctcgatgcga
    13921 actggagtcg gaatttggag caaacgtcct agattttcaa tagctccttg ggtcttttcg
    13981 actgattttt ctagcaggtt aaatttctgc tccagactaa cacgtgcatt ttttatggct
    14041 agattgacca gctgtttttt ctctccacgt tggggtttga gaatcttggt atccaccaaa
    14101 gccttgacag cttcttcgtc aatatcctgc ggaatcagta cctcattggg aactaggtga
    14161 gatttttctt gatagaattg tcccacatag gtcaagaaat cctcatctgg atcattgaag
    14221 taggggaaga gattgacatc gcgctcgatg agcttgccct gacgaacaaa gaaaacctgc
    14281 acacacatcc agcccttatc cacatagtag ccaaagacat cgcgattttg gagatcctta
    14341 gccatgaccc gttgcttggt tcgaagtgtt ccaatggcct gaatcaggtc acggtattcc
    14401 gctgcccgtt caaactccat actttgtgct gttactgcca ttttactctt gagatcatcg
    14461 atgattttgt catcctgacc tttcagaaaa tcagacacct cctgggccat ggacttgaaa
    14521 taggcctcgt cattcttaca gatggtgtgg gccatacatt ggccgatatg gtagtaaaaa
    14581 cagaccttag agggcgggtt ggtacatttt cgaaaaggga aaatcctatc tagaagtcgc
    14641 ttgatttcat tggctgcccc cacatctgga taagggccaa aataaagacc accgtccttt
    14701 ttgacctgac gagtaatgat aagccgtgga tagtgctcat tggtgatttt gataaaagga
    14761 taagacttgt catccttgag catgatattg tacttgggct tattttcctt gatcaggttg
    14821 atttccagca gaagcgcctc aatattagac tccgtaacaa taaattcaaa atccacaatt
    14881 tcagacacca aggcctctgt tttggtatca tgacttccac ggaaataaga cctcacgcga
    14941 ttacgcagat ttttagcctt tcctacatag ataatggtgc cgtttttatc cttgtaaatg
    15001 tagcaaccag ggctggtcgg caagagctct agttttggtt taatcaagtt gttcatagtt
    15061 ccattatagc aaaaaagtag ggaaagatag taccctactc attagtctca tatatttttc
    15121 ggaggtttgc aacatcctct tcctggatac cataaaagga acctgctggt tgcatgacag
    15181 acttctcatc tgtatggtcc aagccaatcg catgacctaa ctcatgttct gccgtatgga
    15241 caaggcgttc ataggaatag ccatagtatg gattggacaa ataataatga ttcaaccgca
    15301 ccgttacgga caagaattgc cctgttaaga gattagtttg actttccgcc tctcctgcca
    15361 caggagtgcc tccgtcgttc atctccgtag ccgtaatatc cgccttacta gactcagtca
    15421 cgagttcaaa gttaaaagca ccagtttgat tccagttctg gattgcttct aaataagcct
    15481 cttgaaagcg tgaatccatc tgcggatcca ggtaaatacg agcagaggcc tgtgcccacc
    15541 ttccttcaga atgctggtga ctatctgtct ggcttaactt aggcagttgc cctgttttct
    15601 cccattggtc aatactttgt tgaccaattt ttactgaccg ttctgcttgc tttagcgctc
    15661 cttgaaaatc cccgttcaga taccagagaa gtccgaaagc aagcacacat aagagcacaa
    15721 ctatccaaac cagacgccaa aacaaacgcc acacaaaaga aaagaaagcc cctatcaaac
    15781 gaaaaagcca gcgcatgtct ctccaccttt ctagcaaata agaattattt tactaaaaga
    15841 agctaaaaga aatctaaata ctgacttttt catcttaacg tcctactatt tttttgagta
    15901 actggatagc tttgtatttt aaaggcgatg gatggtaacg gaaagtaccc gctttgcgta
    15961 caatatagcc attaaaattc tgtttaaaac gcaaaacacc atcacttcca tcaaaaatcc
    16021 cttgaatgcc taggaaattg tatttaggta ttccacgttt tatgctttcc aacataacat
    16081 atttttgaag cagtgcaggg gcatagaact tattaaactc agtgtaggaa ccactaaaga
    16141 gataagtcgt ttcctgaggc atataaacaa ataaactccc agctaaaaca atatcttctt
    16201 ctccatattt ttcaatcaag tctcgcgctt ctgcttttcg aacttcaaac gtttcaaatt
    16261 gactagaata ttctctcagt tgattttgtt ttttctcaga atgaggattt ttactcaaat
    16321 caagtcgcaa cttgtccaag ttttcttcta gtttattttg ttcaccttgc aatttgctca
    16381 tatagtccga aaaatttaag cttgctatga gaaactccgc ttgttctcca aaagtatcat
    16441 aaaaatgctc ataatattct aaacttttat cactatattc tctacgttca gaggtttctt
    16501 ttgttatatt cttaaaaatc gatagttctt cacgttttaa ctttttcaac cgaatgccaa
    16561 aggtttcagc ctttttcacc aagggtttac cctttttgct aaaactttta agcaaactct
    16621 tttcagttaa ttcagttaaa tctttatagt ataaccaatc tggttctcca cctgggtaac
    16681 ctgttgttaa gccatcaaat tgataaccta aatcagtcaa atcttgaata atactttttt
    16741 tctcagcatc tattggatta ccttggctat caaaagtttg ataagtttca tagggtttta
    16801 caagcaactc taatacacca ttttgcttgg catattcttt taactctgca taaaaaactg
    16861 gaagagcatc ttgttgggta taaatcggcc ccgaattgag ttccatatgc agaccaccca
    16921 gcatgggcaa gctataaacc agagctgcaa cttgaatttc tccttcttgt ttcaaagcaa
    16981 gataaacaat tcgagcccct cttttttcta gcaaatcccc catctggaca gattgcataa
    17041 aggaacgaga agaaacctga tcagaataag tctgaaactc ttctttcgtg agtgttgtta
    17101 gtgccatata cttactttct atgttttttt cttaatgttt tacggaaatc aagagcaagt
    17161 cttaacagag gatagagagg atgagtgggc attgtaaatt cacccaagta ttcttcaatc
    17221 gttggattaa atttttcctt aaaatgataa agtccaccat tgagagagtt ttcaacacca
    17281 cctaaatttt gccagatcat acctcgctca aaggcatagc gagccgtttc ataccatgtt
    17341 aaaattggtg cattgtaacg tttaaaatca tcatccatac cagcatatat attgacagag
    17401 gtagtaccaa attccaaact caaagtagcc gctaaaggaa ctctcgcttg acctacatct
    17461 atatattcct gcaagaaggt caattcctct aacaaacgtt ctttttcctt cttctgcgct
    17521 tctacttttg aagttcgagt cgactcagta aacgtctctt ccaaggctct atttttcgct
    17581 aactgttctt ctaattcttg cgaacgttta gaaacatcca aggtggccaa ggtgatatag
    17641 gccttgtcct taaaattatc taacaatttt ttataatagg cttcattcct caaatgaatc
    17701 tcttttcgct tctcagtttt tttcatcaac tccgaaaatg aatctaatag ttccagtcca
    17761 ccatattgaa tctcaagccc tttgtttcgt gctgttcgaa tagtctgttt tgttgacttg
    17821 gaaagtttat cttcttcaaa attttccttg tatattttcg cctgaatacg aggttgaatg
    17881 gtgtctccca tttcctccgt ttttcctgac caccttactc ccatttgttg caaactatca
    17941 ataatagcca gattttcagg aaattctgtc ttttcctgat tgattaaact ttgagatagg
    18001 caaatacttg ggtcaaaagt cacaaaaacc gctctcttac tgcgagcata ggacttaata
    18061 gactgaatgg caaaattcaa gagttcttta tccccataat ccaatatagg tcctcttggg
    18121 atgtaaaaca ttttatagcc tagcggaaga gttctaatca aaatactagc tgtcgccagt
    18181 aatttttctt ccctgtaaac accaaacttc tcatgttgcc aattagactt aacttcctcc
    18241 caagcactac tttgtaatac attggctaat tcatgttctt tgacaaactg atcatattct
    18301 aatgtgggaa tgccaatttg ataacggtac atttcctact ctctttcctc cagtatttta
    18361 ttactattct actactttcg tttgaaaagt ctagtgaaaa catattttat tttagttcta
    18421 ctccccctat gctgaagttc aaactaagtc agtctctctt ctaatttgaa atcaattggt
    18481 agggttgtta aaaaaacttc caattgtttt tcccagtagt accactcgtg agttccagcg
    18541 ctatggctat aggtcacatc aaaacctagt tttttgagat ttttcactgc gagattattg
    18601 gcttcgtaca agaaatcctg ttcgccacac cacgcccaaa gtttggtctt tttatccgat
    18661 tttttagcca gactttcaag agaataggga ctagttgtcc agtctctaat ctctccaaaa
    18721 acacctctcc agtaggctgg acttcccaga ttttgacttt caggagaaaa gttttgaaag
    18781 ctgagggcac ctgaaaaact agctgcatga gaaaaacgat ttgtcgcaag agccagtttg
    18841 aaacagccgt agcctcccat agaaagacca gctataaagg tcttttcacg cttgctagtc
    18901 atattaggga agaagcgttt cagaacctgt ggcaattcct ctgctagagc cgtgtagtag
    18961 tcaaaaccat actgggtatc ggtgtaccaa ccattgctgg tattgggcat aacaacgatg
    19021 agattagttc ctcgaagcaa gcgttctaca ttggtccgct taagccaact attatgattt
    19081 ccagacatcc cgtgcaaaag gtacaagacg ggaatatctt cacactctgg ttcttccact
    19141 cgattggcat cagggtagag gacattcatc ccccactcca tatccaatac ttgtgagtaa
    19201 tactcgattt tcattactgc catgattttc tccttctatt tttctataag aaaaagccga
    19261 aactcgactt tctctctgtt tatttcaacg attattttgc ttccatgact actggtaaaa
    19321 tagctggacg acgcttggtt tggtcaaaga gatacttggt caaattatct cgaaccttcc
    19381 ctttaagatc tgcccagtca aagtcatctc cttgaagata atcttctacc gtttggttaa
    19441 tcaattctga actttcacgg agaatatcgc gactcttctt gagataaaca aatccacgcg
    19501 tgtgaacacg agctctagcc acaattttct tctcacgacg gttgactgta atagccacga
    19561 tgaaaattcc atcctctgac aagaccttac ggtcacgaag aacaacattt ccaacatcac
    19621 caatggcatt cccatcaatt aagatatctc ctgctgaaac cgatccagct ggaacaaagt
    19681 ctccattctc gtaagccatg gtcgtcccct ttttaggaat gaagatgcgt tctggcaaca
    19741 tcccaactgc catggcagcc ttagcgtgag catccaactc acgatactcc ccttggacag
    19801 ggaagaggta cttaggttgc aaaagattga tcatcagctg caaatcacgc acatttccgt
    19861 gccctgatac atgtaaactt tgggtaatca atttgacaac cccacctgcc tgataaatca
    19921 tattttctac acgcgcaaca aaggcttctt tagcaataga cggagccgta gcaatataga
    19981 ccaggtcccc atccttgatt tctacataac gatggcgacc aatcgacatc ttacgaagtc
    20041 cattgatagg ttcacccata cgacctgtct caagaataat caactcatgg tcttcaaagc
    20101 gagacatatc tttaggctta atcaagagaa tttcgttggc taaagacaac ttcttaagac
    20161 gaatcgctgt gcggacgata ttttcaatat caaatcctgt caagacgata cgtcgacctg
    20221 ttttatccgc agcgtcaaaa atctgctgaa tacgagaaag attactggaa acagctgcaa
    20281 cgatgatacg accttcccag tcagcaatag tttgggtaat ttcatcccta acttcacttt
    20341 cactagccac ctgaatattg ctgtctgcat tggccgaatc actgaggaga gccaggacgc
    20401 cgtcacgacc aatctctgcc aaacgagcaa aatcagttgc ataagattca ctagccgttt
    20461 ggtcaaattt gaagtcacct gtataaacga tgcttccttc cgatgtcttc aagacaattc
    20521 ccagactctc tggaacggag taagtcgtag ggaagaagga aaccactgtc ccaccaaaat
    20581 caatctccgt attctcatca atgacatgga aatcattaaa tttcttaacg gcatcatttc
    20641 ctttgacaaa gagctttgcc aactcaatgg tcaactcaga cccaaataca ggaactttag
    20701 cctctgccaa gagataaggt agagcaccaa tcgcatccgc atgcccgtgg gtcaagaaaa
    20761 ccccagcaat acggtcgcta ttttcaaaaa ggtaatccat gtttggaatc accacatcga
    20821 cccctaattg ttcattttca ggatatttta accctacatt caaaacaaaa atggactctc
    20881 caatttcagc aatgtacata ttttttccat tctcacgcac accaccaagt gttgttaaac
    20941 tgatattact catttttcct ccaaaatctt agtttatctt gattataggg caactctacc
    21001 ctcttattct aaaagttcta atactttcag ccgaatcttt tcctaccatt ataccatttt
    21061 tttgatgatt ttcaaaatga aggtctgttt tcaaaaaaga gttgatttcc caactctttc
    21121 ctaacttcta ctctttttcc atcaaaaaat cataaatttc ttgcaccata cccagcgcca
    21181 tcatttcttg gtctttaacg gtttgtttga gattttggta aatttgactt tctcctatct
    21241 cccgctttgg cgcctcttta ttgactgtca tcacaccatc ttttatcgtc acaaagccta
    21301 gttcttcaaa tacttgaatc atcttgacca gcaagatttg ttgaatatta agatatgcag
    21361 ccaaatcttt cagcttgtag cgaatatcaa actctgggaa ctggtaaata gtcttgtaca
    21421 atttggcaaa ctgatctcta gtcccataac ctgtcagata ataggccttg tcaatatcat
    21481 ttttgaaata gacagcagag aaatgctgtt cctgaaaaat ggtcttcagc tgagtaatat
    21541 cctctggaat gttttttacg acaatagctt cactagccgc aagatttggc agttctccag
    21601 gaaaatccaa gactggaaca ccttctggca agactgcatt ttttccacga atgttaaaaa
    21661 gttgaacacc ttccactcgc gcatccacca tcatcaactg gagggcagtt tggccattcc
    21721 attggttgac agacaattta accgctaact ctagattctt ggtttgagaa aactctgtcg
    21781 cccatctgcc ttgaccaaag gctaccactt caaaactcgc ctcacccttg gaaattttca
    21841 gctttagatg ggcattacct gcccccatag tacgagcact ttcgacctga aaattcttga
    21901 tataaaaaat aggtttctga ttatccattc caaaaggagc taaacgttca aaacttttga
    21961 ccgtttccaa gctaagtgcc tccaaatcca actcttcatc taggtttaac ttattcttgc
    22021 caccagcatc tgcacctttt tcacgaacat aatcttccaa aacctgagat aaatctgaga
    22081 gttgctcaac ttccagcgtc atacccgctg cacctgcatg acctccaaag gcgataaaga
    22141 ggtctcgatg gggatccaga gcttcaaaaa tatcgaccgc ttccacacta cgagcactgc
    22201 ccttggcacg accgtcttct atattaagaa caatgactgt ctgtcccaat tcttccaata
    22261 aacgaccagc cacgattcct agaaccccag gattccagcc ttccttggcc aagacctgaa
    22321 ccttcttctc aggatccacc atggtcttgg cttcttcata gatagactga acgatttcct
    22381 tgcgctcttc gtttttctgg tgaatcataa aggcaatctc atgcgcttcc tcatcatcaa
    22441 atccagtcaa caaatcaatg gcaggattgg gatcatccaa gcgacccaag gcattcaaac
    22501 gaggagcaat ctggaaacca accgtttctt ctgttacttc gttggcagca atcccagcca
    22561 tgtccagcat ttcttgcaga ccaatgcgct gggtatgacc caacatttcc agaccatatt
    22621 gaactaagat acgattttca tccgtcagac tcaccatatc tgcaatagtt ccaatagcga
    22681 ccaaatcaag caattccact tgcacttctt ctaacagggc acaagccaac ttgaaagcaa
    22741 ctccacaacc agccaaatat ttaaaaggat aatccgcatc tggatgttca ggatggacaa
    22801 tagcataagc atctggcagg gtttcaggca tggaatgatg gtctgtcaca atgacatcta
    22861 ctcccataga ctgagccaat gcaatagcct catgaccagc aaccccattg tccaccgtca
    22921 caatcaagga aattccttct tgctcgataa agtatttata aacactagca ttagggccat
    22981 agccatcggt aaaacgattt ggcaggtaaa ctcggcactc agcaccaagt tgttccaaac
    23041 tttccttcac aatagaagcc gaagtcatgc catccgcatc atagtctcca taaacgagaa
    23101 tattttcccc ttcttcaata gcctgacgaa tccgctccac tgccttgtcc atatcatgga
    23161 gcagataagc atcatgtaag tcctccaagg aaggttctaa aaacttcttc agactttctt
    23221 ggtcctgaat ccctctctca aacaataacc gagccacctc aggacccagt ccagccttct
    23281 tggctatctt tgtaaaatcc gcatcttcta cctgcagggc aaactgccat tcataagtag
    23341 gtgttatcaa aaagacatcc actctttcta agaattctat tgcttcatta taacatgatt
    23401 tagacgtttt ctctatccag attgcttttt tataacattt tatagtatat tgaaactaga
    23461 atagtacacc tctacttcta aaatattgtt agaaatcgat ttgactgtcc cgatcgattt
    23521 gtcctgttct tatttcacta tagtgaattt tttcagatat ggtttgacaa gaacattctt
    23581 ttattgtaga atcgtgttat aaaatctaac acaaaggaga tctctcatgg ctttagtaga
    23641 atttaaaaac gtcgaaaaat attacggaga ctaccacgca ctccgcaaca tcaatctccg
    23701 ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct ggctctggga agtccactct
    23761 tatccgtacg atcaatggtt tagagactgt tgacaaagga agtctcctag tcaatgggca
    23821 ccaagttgct ggtgccagcc agaaagattt ggtacctctt cgcaaggaag tcggcatggt
    23881 ttttcaacat tttaaccttt atccacacaa aactgtgtta gaaaacgtaa cacttgcacc
    23941 cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa accgcccaaa aatatctgga
    24001 atttgtaaat atgtgggaca agaaagattc ctatcccgcc atgctatctg gtggacaaaa
    24061 acagcggatc gccatcgctc gtggtcttgc tatgcatccg gaactcctcc tctttgatga
    24121 accaacatct gctcttgatc ctgagactat cggagatgtt ctagcagtta tgcagaaact
    24181 ggcgcatgat gggatgaaca tgatcatcgt tacccacgaa atgggctttg ctcgagaggt
    24241 tgcggaccgc attatcttta tggccgacgg agaagtttta gtagatacga cagatgtcga
    24301 taactttttt gacaatccaa gcgaacctcg tgcccaacaa ttcctcagca aaattatcaa
    24361 ccacgaaagt gacaaagtca aataaggagg cgcctatgaa aaaaaaattc tttttatcag
    24421 cattattgat tagccttttc ggccttgctg ctgccaaacc agtccaggct gatactagta
    24481 tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt caaacaagac gttcccaatt
    24541 ttggttacaa agatcccaag accggtactt attctggtat cgaaaccgac ttggccaaga
    24601 tggtagctga tgaactcaag gtcaagattc gctatgtgcc ggttacagca caaacccgcg
    24661 gcccccttct agacaatgaa caggtcgata tggatatcgc gacctttacc atcacggacg
    24721 aacgcaaaaa actctacaac tttaccagtc cctactacac agacgcttct ggatttttgg
    24781 tcaataaatc tgccaaaatc aaaaagattg aggacctaaa cggcaaaacc atcggagtcg
    24841 cccaaggttc tatcacccaa cgcctaatta ctgaactggg taaaaagaaa ggtctgaagt
    24901 ttaaattcgt cgaacttggt tcctatccag aattgattac ttccctgcac gctcatcgta
    24961 tcgatgcctt ttccgttgac cgctctattc tatctggcta cactaataaa cggacagcac
    25021 tactagatga tagtttcaag ccatctgact acggtattgt taccaagaaa tcaaatacag
    25081 agctcaacga ctatcttgat aacttggtta ctaaatggag caaggatggt agtttgcaga
    25141 aactttatga ccgttacaag ctcaaaccat ctagtcatac cgcagattaa ggaggatacc
    25201 ctatgacaga tttatcatct tggacagcct attttcagga ttttggacaa tttttcaatg
    25261 gtttcctctt tactcttgcc ctagcgattg gatcctttat cctcgccatg gtcttaggaa
    25321 tcttctttgg tgccatgtca accagtaaac gtccaatttt acgcatttta gctcgcatct
    25381 ttgttgaatt ttaccaaaat actcccctct tggtgcagtt tgttattgtt ttttatggcc
    25441 tacctcttat cagtgaccat atcatcatga ttccaattta ttggacagcc gttctctgcg
    25501 tgggactcta tcacggcgct tatatcgctg aggttattcg ttcagggatt cagtctattc
    25561 ctagtggtca gatggaggcc gccttgtcgc aaggttttac ctatatcagt gccatgcgct
    25621 tgattatctt gcctcaggcc ttccgcatca ttctccctcc attgaccaac caaattgtta
    25681 acctcatcaa gaacacctct actgtagcta tcatctctgg agtagacttg atgtttgtga
    25741 ctaagtcttg gtcggctctc aacggaaact atatcccagc ctttttaggt gctgctcttc
    25801 tctactttgc cctatgcttc cctgttgccc agtttggtcg caagatggag caagccaaca
    25861 aaaaagccta ttcactttag gaggttacta tggaatctat tttagaagtt ttaaccccag
    25921 ataacctagt ctttatcttt aaaggatttg gcttgaccct ctatatttct ctgattgcca
    25981 tcatcctctc tactatcatc ggtacggtgc tagctgtcat gagaaatggc aaaaatcctg
    26041 tcttgcgaat catctccagt atttatatcg agtttgtgcg caacgttccc aaccttctct
    26101 ggatttttac tatctttttg gtgttcaaaa tgaaatccac accagcaggt attacagcct
    26161 ttactctctt tacatcagca gccttggttg agattattcg aggcggtctc aatgccgtag
    26221 acaagggaca atacgaagca ggaatgtcac aaggcttcac ctcagcccaa atcctctact
    26281 acatcattct cccacaagcc atccgcaaaa tgctaccagc catcatttct cagtttgtta
    26341 ccgtgattaa ggataccagt ctcctctact ctgttatcgc cctacaagaa ctctttggag
    26401 ccagccaaat tctcatgggc cgttattttg aaccagagca ggtcttcagt ctttacatcc
    26461 tgattgccct catctacttc agctttaacc tagcaatttc tagcctgtct catatgctag
    26521 ccaaacgttg gcaacaagct gcagaataat actcttcgaa aatctcttca aaccacgtca
    26581 gctctatctg caacctcaaa acagtgtttt gagcaacttg cggctagctt cctagtttgc
    26641 tctttgattt tcattgagta taaacagcca ctctccagtt caattggaga gttttttgat
    26701 gagagtagat atttatagac ctcaatttga caccatacat tttctatgat aaaatgtaac
    26761 aaattcattt acaacaaatc gaaatctaaa tgaaatggaa ttttctatga aatcagtcaa
    26821 aggactactc tttatcatag ctagttttat cttgactctt ttgacttgga tgaacacttc
    26881 tccccaattc atgattccag gactagcttt aacaagccta tctctgactt ttatcctagc
    26941 cactcgtctc ccactactag aaagctggtt tcacggtttg gagaaggtct acaccgtcca
    27001 caaattcaca gcctttctct ccatcatcct actaatcttt cataacttta gtatgggcgg
    27061 tttgtggggc tctcgcttag ctgctcagtt tggcaatctt gccatctata tctttgccag
    27121 catcatcctt gtcgcctatt taggcaaata catccaatac gaagcttggc gatggattca
    27181 ccgcctggtt tacctagcct atattttagg actctttcac atctacatga taatgggcaa
    27241 tcgtctcctt acatttaatc ttctaagttt tcttgttggt agctatgccc ttttaggctt
    27301 actagctggt ttttatatca tttttctata tcaaaagatt tccttcccct atctaggaaa
    27361 aattacccat ctcaaacgct taaatcacga tactagagaa attcaaatcc atcttagcag
    27421 acctttcaac tatcaatcag gacaatttgc ctttctaaag attttccaag aaggctttga
    27481 aagtgctccg catccctttt ctatctcagg aggtcatggt caaactcttt actttactat
    27541 taaaaattca ggcgaccata ccaagaatat ctatgataat cttcaagccg gcagcaaagt
    27601 aaccctagac agagcttacg gacacatgat catagaagaa ggacgagaaa atcaggtttg
    27661 gattgctgga ggtattggga tcaccccctt catctcttac atccgtgaac atcctatttt
    27721 agataaacag gttcacttct actatagctt ccgtggagat gaaaatgcag tctacctaga
    27781 tttactccgt aactatgctc agaaaaatcc taattttgaa ctccatctaa tcgacagtac
    27841 gaaagacggc tatcttaatt ttgaacaaaa agaagtgccc gaacatgcaa ccgtctatat
    27901 gtgtggtcct atttctatga tgaaggcact tgccaaacag attaagaaac aaaatccaaa
    27961 aacagagctt atttacgaag gattcaagtt caaataagta atcatactct cctgggattt
    28021 cccaggagag ttttttatag ccaaatgaaa taagacagga acgatggaat tttggcattc
    28081 aaatcaattg ataagaatgt tttatagtgg agtgaaatag gatacgaaca aattgattag
    28141 gaaaatcaaa tgaatttata gaaatctttt agcagttatg ttgtcctatt ctagtttcaa
    28201 aacgctatag aagcagcatt gtgctagtct agattcagtt tactatacta aaacgagtag
    28261 cttgaaatca aaaaacccac cctcacaggc aggttttatc tgtattattc agctagatta
    28321 tgctttacct tctgaaccga atacgtcgat acgttcttca accgatgctt ggatagcttt
    28381 tacaccgtca gccaagaatt tacgtgggtc gaagagtttt ttcttgtcgt attctgcttc
    28441 gtttgcttcg tagtcacgag caaatttacg agttgcgtta gcgaatgcga tttggcattc
    28501 tgtgttaacg ttaactttgg caacaccaag tttgatagct gcttggattt gctcatcagg
    28561 aatacctgat ccaccgtgca atacgattgg gaatcctgga agagcttctg tcaatttttg
    28621 caagtggtca aggtcaagac cttcccagtt tactgggtaa ggaccgtgga tgttaccgat
    28681 accagctgcc aagaagtcga taccagtttc aaccattgct ttagcgtctt cgattggagc
    28741 caattcacct ttaccgatga ttccgtcttc ttcaccaccg atagtaccaa cttcagcttc
    28801 tactgagata cctttagcgt gtgctttttc aacaacttct ttagccaatt taaggttttc
    28861 ttcaactgga aggtgtgaac cgtcaaacat gattgaagta taaccaactt cgatacactc
    28921 aagtgcatct tcgtagtgac cgtggtcaag gtggatagct actggtacag tgatacccat
    28981 tgattcaaca aggttagcga tcaagttgcg agcaactttg taaccaccca tgtatttagc
    29041 agcacccatt gaagtttgga tcaaaactgg agctttttta gcttctgctg cgcgcaagat
    29101 agcttgagtc cactcaaggt tgtttgtgtt aaatccacca actgcataac cgttgtcacg
    29161 ggctgcttgg acaaattttt ctgctgaaac gattgccatt ttatcaggcc tcctgtatat
    29221 ttttatgggt catcccattt acattgttca ttttatcact ttttgccaaa aaaatctagt
    29281 ttttcccgca gtttcgattg attttcttct aactccatct atgtaaaccc tttctctccc
    29341 tagtcttgga cgacttttgg aaaatctata aagaaggtta aacgattctc ctccatctcg
    29401 aaacgataag ctaatttttc atgttctaat agactcttaa ccacaaagag ccccatacca
    29461 gaccccttga ccttgcgact ggcattgtca gaaaaagact gggctagttt ttcttgttcc
    29521 tctgagctac agctattttc gataaaaagt tctccttctc tttctccaat tcgaactaag
    29581 ccacctggaa cagagtgctt aatggcattg ctgatgagat tagaaagaat caacttcata
    29641 actgatgggt ttagataagc ctgctgatgg gtcaaactat tgtctatctg gagctctctt
    29701 tccttggcta gcaaggcata atctttgacc agattttgcg tcatctggag gaggtcaatt
    29761 gtttccctat catctcgcaa ttcctgcaca gaagagaggg aaagtatctg cagaacatgg
    29821 tgattgagtt catccacaat ccccaaggca actcccagat actggtctct atccttataa
    29881 cgaccgatat tctctctcat attttcgatt aggattttca aactagccag cggtgttttc
    29941 aattcatgag aagctcctcg taggaattcg accttcatct tctccagctg gagaatggct
    30001 tcattctttt catgcaagtc cgcaataaca gtcaagagat gctggtagag gctattgatt
    30061 tgttccttga gattacctat ctcatcctta gaatccacgc gcaatcgcac ttgggcatcc
    30121 aggtacatca tccgacgagt cacccgcttg atttccaaaa tcggtgcaac aatagtccga
    30181 gcgtagatgt aggccaccaa gagggaaatc agaaaggagg ccagcaaggt atagggaaga
    30241 aactggagac tgatttgctc cgcttccttt tgtaaatcca tggaagctag aaactggaga
    30301 atcatagtac caccgtcttg cgttttcacc tcgcgctcct caataaagag agaggttgtc
    30361 tggcggtctg tgtccagagg aagactgtcc ttgacttcta acttgtcctc ggtcatctca
    30421 cctttgacgg tccccttgat atcactagtc tgggaataca agtctaacac ttgctcgata
    30481 ctctgcctat ctttcccttc tagggactgg gcaatggctg ttgccttttg accaatggtt
    30541 tcctgacgat gactcagata agtcgaagga aaaagaaaat aaatagctaa atgaaggcag
    30601 ataaccagaa cactaaatat cgagaaggta tagataaata tctttgcaaa taaacctgtt
    30661 cgtttcattt tcgctccaat ttataaccaa cattgcgcac agtgaggata caatccaagt
    30721 ctagcttttt ccgcaattcc ttgatataaa catcaataac acggtcaaag ggaacctcat
    30781 ctgtcgcttt ccagacggca tcgataatct gagatcgagt caaggcccgg ccttcatttt
    30841 tcactagata gtccagaatt tccaactctt tggcattgat aggcacttct tgacctgcga
    30901 tgcttgcact gtagctttca aagtccacct tggtatcctt gtaagaaaag attcgtcctg
    30961 tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt taaaagggag agggagaaag
    31021 gtttttccag atagccatct gccaaagagg caaaggcact catcttgtat tcctcatcct
    31081 gaaaagctgt caacatcaag acaggaacct gactggtttt acgaatctca gctaggactt
    31141 ctaagccgtt gagcttgggc atctggatat ccagtaaaac cagggccacc tcatagctag
    31201 aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat agtttcatag ccacaatccg
    31261 tcaaataatc actgaccccc tcacggatca tctcttcatc ttctacaatt aaaattttca
    31321 tactttaact gctctctatt ttttattttt cttagaataa atacctactc tattttctat
    31381 tatagtctct tgctggcctt ttgtctgtaa gtaactgacc actagataaa acgttgtgaa
    31441 attcctttct cataaattcc ataactttag tatattatat ttaagcacta aagtacaaag
    31501 aaagcaactg aaagcaatga ttttcaccac tgctttcaga tttattttga attgttaaat
    31561 agccattcct atccactatt cttgaataga aacacaagat gcaatcttta ttccagactc
    31621 attttttcaa atttattcac catccagcaa gagttctttt ggttgttttc taaggagatt
    31681 gcttgaagca agcgccataa cgagaaccac tagaaccaag gcaaggacaa aaatgatgat
    31741 aaagtctgat gtctgaatgg aaatgtctag gctcgacaag gtcttgctaa agccatctac
    31801 ttctgcacca ccaccaaggt tagaggcttg agccgcctta ctagcctgtt tggcaacacc
    31861 tgaagtcaca ttggcaagga cagtgtttcc aattgcacgg gcagtgtaat tagctaggaa
    31921 gtaagcagaa actagagcag ggatagcaat caagatagat tcggtgatga attgacccaa
    31981 gatacttacc tgcttgaggc cgatagagag gagaattccc acttccttgc gacgggcgtt
    32041 gatccaaagg ctgagcaaga gggcaaggag gagaactgag aagctcaagc taccccagaa
    32101 gaggaggttg gccatcttgt acataccaga gatagattgc tcaagagctg ggtagttaga
    32161 ggagctcttg acgagtgtgt agctcttcca gttgatacca ctgatgccat tcaactcttt
    32221 cataacatca tccaagttct tgtctgctgt tacaaagaag gttgcgtccc cataaatggc
    32281 tgtgtcttct gtgtatccat aaagttttgc agcagtgtga atgtctgtaa tagctgtgtt
    32341 ttcgtaaagt tcttgtgagt aggttactgc tgacttatta tgaccatcaa agagtccctt
    32401 gattgtcact tcaactgttt ccttggctcc tttttcatta tctgcatcgt agatattaga
    32461 gtccagttta accttgtccc ctactttcca gccgtgtttg gctgccaagt ccttgtgcaa
    32521 gaggatttta tccttgtcgt cgttggttaa gtgctctcct tcgactagtt tataagaacc
    32581 agagacaaac ttgtcttctt tagaggagtc attgacacct gtaatcatca agctacttcc
    32641 aaaacgtttg gcacgatcag cagtgagatt cttcttggtt tctggcgttt caatcaggtc
    32701 atatccagtc aaatctccga tagcgttgat acgtttgaca taagactcga tggccttgtt
    32761 ttcggtgatt tttttgatgt cttcaccctt gatattccca gcaccacgag gcgttccttg
    32821 gttgacgcga cgattgattt gcatggagaa gctattggtg atatttttaa aggtctcctg
    32881 agaagccttg gcagtagctc ccttgattga caagccgacc aaactcaagc tcgccatgag
    32941 gagaataatc aggaagatga caatcgattt gaaaaacttc cttgtaacat aggcaaatgc
    33001 gttgtgtaac atagattccc tttctagatt ttgttttaat cattctatca aaataagctc
    33061 aaattattta ctagtattgc gcgtttcagt cagtttctta tcctttaatt caagtgtaat
    33121 atctgacgct tgtgccactt ctttactgtg agttacgaca atcacacatt tacctgtttt
    33181 ctgggcaagt gatttgagta gttcgacaat atctccagca gttttaggat ccagatttcc
    33241 tgttggctca tcagctagaa taactggagc ttctgagacc aaactgcgag caatggcaac
    33301 acgttgctgt tgaccacctg ataactggag aacattccgc ttgatctggc tttcatccaa
    33361 accaagctca agaagtgtat tcttgcttgc ctttttgttg accaatcgga tattttccag
    33421 cggagaaaga taatctatca agttataatt ttgaaagacc agggaaatat ggtgcatgcg
    33481 atggtaagaa tagcccttct tacgaatatc ctctccttga aaaaggatag aaccttcaac
    33541 aggactatct agaccagcaa gtagggacaa gagtgtggat tttcctgctc ctgactcccc
    33601 aataatactg taaaattttc caggttcaaa attataatcg atctgatata ggactgcttc
    33661 agcagtattc ttataacggt aggtaacatc ttgtaattgt aataaagtca tgatttctcc
    33721 ttcttaacta atagatgata aaatttcttt cggtgatttt ctaaataaga ataggaaaca
    33781 aagggctaca gataagcaac taagcagaac tagaaaaaca taggattctg caaaagataa
    33841 gatgctagtt gataaactgc ttgctttggc tagtgtatct tgtaagcttg cctgatctcc
    33901 acttgctagt agagtttgga gtaggtaagt tgtgattccg tttcctgcaa caaatgctgg
    33961 aagcaaagct ccaagagata ccaaaactac ctctaaacag aattgtagga agatcgagct
    34021 cttgcctttt ccaagtgcaa gtaaaatccc cacttcatag acccgttctc tcaaccagag
    34081 agacaaaacc agaattaagg ctccagctcc tgctatcaac atcccataaa ggaagatggt
    34141 caggaaggtt tggaaagttg caactgagtc tttgatttgt tcaaaagcct tgttttcctt
    34201 ttcgacttgg tagccttgat tttccaaggc caagttttct acctgcttca tgagtccgtc
    34261 catttcctta ggattttcta catagaagcg tgctgcactg acttgagctt cactattgcc
    34321 caaaagggtt tggctacttt catagtctgt aaagacttga ttttcactga agtcagaaga
    34381 caagcctgtg aatttctctt gttttttacc agaaaagatg ccaataatct caaactctac
    34441 tgtttgtcct tttccagatt cagactgacc agcatccaag ccaatcttgt catgaagtga
    34501 aagaccgttc ttcttagcca attcttcgtg gataaggatt ttcttggaat cccctttttg
    34561 aaggtgtcgc ccttctttta gattgaaagc cgaactggta aaggttacat ccttggatga
    34621 atcctcaaga gccgttaagc taaccaagtt attgtctgca gctgataaat catcacgctc
    34681 cacgctctgc tctccagtca ctgcttcctt gtcttttagt tttgcgaccg tctcaagttc
    34741 aggagagaca ttttccagcc ccttaatctt gcttacagat gctaggtctg acaacttgaa
    34801 tgtctgacca ttctctatct tcttaataga aaaagatgta ttgagtgatt tataaagatt
    34861 gctttctact gttttgttgg acttcatcag agtcaaacag gctgaaattc cggccaataa
    34921 gaccaataaa atcagaaata aaataaaact tctcagtcgc tttctgctga cataagccca
    34981 agatctttgg attggattca tttgtcacct ccatatttgt aagactatta taaaacccaa
    35041 atatgaaatg tttatgaaat acgaaaaaaa aatatcgagt aggggataat ctctagcccc
    35101 tctcacacca ccgtacgtgc cgttcggcat acggctgttc aactaacttt taacgcatgt
    35161 cgttcaaggt aataatccaa acacgaaacc agtccacgtt tttcaaggac tggttttgat
    35221 atagcacgtt taagtaccga cttctgagct actatagtag attgaaacta gaataatata
    35281 cctctacttc taaaatattg ttagaaatcg atttgacttc cctgatcgat tcgtcctatt
    35341 cttatttcat tttaatataa ttgatagtgg tcgccccagc cagatacctt atctgctatc
    35401 catttaggaa cccctaactt aagcaatccc cataatcgtc tcgatttctt cttccattgc
    35461 ttccagataa tcactcgtag gcgagtacgc aagcgctcat ctatgctagt gactatactt
    35521 ttcatattta taattcattc ctttcgtttc actcaaggca caacacagaa tgaaaaagtg
    35581 ttgtgatctt tattttgttt tataataata gtgagaaagc ctatcactac tacaaatcac
    35641 ggggaggtga ataagtgagt ggtacagcca ctacctcgca tattttgtca catcatttaa
    35701 cggtgcataa taagttgtac catctgaata agttgctaca atatcatttg catgctctcc
    35761 ttcaccttta gcaaaggttg gagctcctgc tggatgattt ttatttgcct ctttcaattt
    35821 ttcaataatg gcattttttt ctgtatcttt tatattatca ggatttttca ctaagatttt
    35881 gtctggatat gtcggtttag cagaaacaat ttttactgtt atttcttttt tattcgaagc
    35941 acttgtccag tttccagcat tatctttagc atttaatttt acagtaattc ctgaactagg
    36001 aacttcagta gcaggttgat tatcaacatt attcaacttt aatttcaaaa gagctgttgc
    36061 atcagacgtt ttatcaatcg ttgtatataa tgatgaattg ttattataac cagtttcttc
    36121 atatttagct gtttgtgagc tacttgaaac agaactgaaa ttatgcccac tacctccctg
    36181 attatcttca atgcttacgt ctaaatgaac ttccccacta ttatttggct tagcaacaac
    36241 tgttatagta aaataacata aaatttgcat aaatagatta gggaaatcaa agcaacttct
    36301 aggaatgttt tatactcttc gaaaatctct tcaaaccacg tcagcttcac cttgacgtac
    36361 tcaagtacag cctgcggcta gcttcctagt ttgtactttg attttcattg agtattatca
    36421 gtcacagtgt actttcccag catcaagcca ctataactct gcacataaaa atggagaaga
    36481 tggcaatcct cttctccaaa tattaacttc tttacaaacc aactatagtt gacaaagaac
    36541 ctaaaatcaa ttgataacac aaggtcaggt cggtcaactc tttcaactga agccctgtca
    36601 actcttccca tttatcaatc ttgtattgga gagaattgcg gtgcagatag agttgctggg
    36661 ctgttttagt gagaacagca ctattttccc aaagagagag aatgatttcc tgaatctgat
    36721 cttgatccaa aatcatctgg tgtagacatt ctttaattgg cttcaagtcc acgagtcttt
    36781 ctcccatact ccaaagatag agctgagaaa aagtatgaac accttggtga ccctgacgcc
    36841 accatgtctt gaacaaatcc cgctcagctt tgattaagtc tgatagggct tgatgtcctg
    36901 tctgagacca aacctgaccc aacatgatag aaagacgaag tccaaaatca tactcaaccg
    36961 cttcaatcgt atcacttaaa atatctctta cagaagtgta tttgtcttgt tgaagcacga
    37021 aaacataatc ctgagctccg acctgtagca ctgtctgaca attcggaaaa agagtccgca
    37081 tcatatctag ccaagaagtc agattttcct gctgaaaata agaaagatgg caataaacca
    37141 actgaatctt tttaaaaact tgcggtgcct gtcccttgcc ttcaaccaga taggaatacc
    37201 aagggtttag cgaacgagcc tgctcctgct gggtcaaaag ggcaaccaac tgcttttcac
    37261 gctcgctgag cccagcttcc tccagcaaaa tccactgctg agaagctaaa gggagcgtga
    37321 gatagccctc tttctctact ggttggtctg aaatccgagc ctcaggaaac cagtcttgta
    37381 gttctttttc cctcatgttc tagccctcca ctttttggat gcaccatgaa accaaactct
    37441 caagacgttc cagattctca gtcatatgga gatagcccat aaccgcttca aatcccgtgg
    37501 acatacgata agtcacgaca tctgcatttt tagcctttgt gtggctattg gtattgcggc
    37561 cacgtttgta gatttcttct tctttttccg ttaggacctg ctcctccaac atgagagcaa
    37621 tcaggcgagc ctgagccttg gctgacacat acttggttgc ttcttgatgg agtttattgg
    37681 gtttggtcat acctttgagg atgaggtggc ggcgaatata catagaatac accgcatctc
    37741 cctcaaaggc tagcgcaatc ccgttaatga gattgacatc aatcacgtgt ccacctcact
    37801 ccatcttttg tatccaatag tttaatgcct tgagcagcca gttggtcacg gatttggtct
    37861 gctgtctcaa agtctcgatt ggcacgcgcc tcttggcgtt tttgaatcaa gtcttcaatc
    37921 tctgcatcca aaacttcctc aacaaagaca attccaaaaa tttctaacat atctgcaaga
    37981 gcttgcttga cacttgcatc atagttccct gagttgatcc atttggtcat ttcaaagaca
    38041 actgtgatac cgttggcagc attaaaatct tcatccatag ctgctacaaa cttatcttta
    38101 aagttttgta actcttgggc atccacgttt cctgtaaatg gttgttcgta agtattcttc
    38161 agatacttga gattggtctc ggcatcgcga actgcctttt ccgtgaagtt gataggctta
    38221 cggtagtgct gggtcgcaaa gaagaaacga agtacttgcc catcaagagt tttaagggca
    38281 tcgtgtaccg taatgaagtt acccaaggac ttagacattt tgacattgtc gatattgaca
    38341 aagccattgt gcatccagta gttagcaaaa gccttgcctg ttttagcttc agattgggca
    38401 atttcattgg tgtggtgcgg aaattctagg tcagctccac caccgtggat atcaatggta
    38461 tcacctaaaa tctctgtcga catgactgaa cactcaatgt gccaacccgg acgtccaggt
    38521 ccccaaggac tatcccaaga aatctcacct ggtttggaag atttccatag agcaaaatct
    38581 acaggatttt ccttacgagc cgtttcttca tcggtacgac ctgaagcacc tagatccaaa
    38641 tcttccaagg ttttattagc caatttggca tagttgtggg atttttctac acggaaatag
    38701 acatcccctt gactctcata ggcaaagcct ttctcgatca agtcttccac aaaacggatg
    38761 atgtctgcca taaactccac tacacgcgga tggcgagtcg caggtttcac gcctaaggcc
    38821 gtcacatcct cacgaaaggc agcgatgtac ttatccgcaa cctcctgagg cgtgatacct
    38881 tcttccctgg cacggttgat aatcttatca tccacatctg taaaattgga aatataggca
    38941 accttatacc cacggtactc aaaatagcga cgaatcgtat caaaagctac cgtcgaacgg
    39001 gcgtttccca cgtggatata gttgtacacc gttggcccac aaacatacat cttgatcttg
    39061 ccgtcctcaa tcgggacaaa ttctcgcaaa tcacgagaca tggtgtcata gattttaatc
    39121 ataaatcata atcaggaaag ctgaaatcca agaacaatta gtttcatcac taaaagttca
    39181 agtaaatttc agtccgaata tctctacact tcggaatccc ttgctccttt ctcattcaga
    39241 taaaccacct gagtctgttt gacaaagcca attttttcat acaaacgttt ggcacctaca
    39301 ttgctatctt ccactgcaat ctgaaattcc ttgtcatttt gctcaattag ttggttgacg
    39361 agggattttg ctaagtagct tccatagcct ttcccacgtt caggttccga tattgctaaa
    39421 ccgtagaagt aattcgtatt agtcgataaa tctaccgtgc aagtcccaat aacctgaccg
    39481 tcttttaaca aaatatataa gcgactttct ggatccttca gagcttcagc gacatatcta
    39541 tccacaactt ctctcgattc atgttcctct gaaaatgcct gaaattttaa ttgactaatt
    39601 tgatcctgat acgaactatc tgctaacaaa acttcaagat gggaaacatt tgctaacgga
    39661 taaggtcttc tatccttacc taaccaagtt tctgtctctt catcctcgat tagtccccag
    39721 ttactggcaa agtcaggatg attctctaaa aaaatacgtt ctgtctgaaa agtgactgac
    39781 cgaatgggga aagaagctat ttctctctca aaactagtaa acaatgcacg cgcaatcccc
    39841 tgacggcgat gacctggatg aaccagtatc gttacttcta catcttggtc atctgcatag
    39901 acagttaata aaccaacaag ttcgcctttt tcataataaa ggaaaaaggc gggcatgttt
    39961 gggtcaaaat taagcatgtt agagagatag ggatcgcgat aggtaccgtc atagttttgg
    40021 caacagttaa ttactttttt cgcctcagat agctcctctt ggcttaactt gtttcttgct
    40081 tgaatcatat aggtatcctc tacaaaccag acgatctgtg actggcatct ttagcctgct
    40141 cgagtttatt gacataatac tctcgttttt cttcgacttc gtgaatgaca ggctcatctt
    40201 tcttaccatg aagacggaca atcttggccg gaataccgac aaccgtcacg tcactaggta
    40261 catctgctac gacaactgct gcagcaccga ccttggcatt ttcaccaatt tccacaggcc
    40321 cgataacttg ggcatgggct gatatgaggg ctccctttcg tacagtcgga tggcgtttgc
    40381 cacagtcttt ccctgttccc ccgagagtca ctccgtgata gagaagaacg cctttttcaa
    40441 caatcgctgt ctctccaatc accagaccag aaccatggtc aataaaaaca cctgaatcaa
    40501 tctgggctcc tggatgaatc tcaatctgag tccaaaagcg ccaaaactga ctgtacatac
    40561 gagctaatag tttgaagccg tgcttccaga gaaaatgcga gagacggtgg gccgccaagg
    40621 ccttgacacc tggataagtc agcaaaacct ccaaagtggt gcgggccgct ggatcatttt
    40681 cttttacaat atcaatggtt tcgcgccacc accccataca tttctccttt tcttattctg
    40741 aatcttttgg tgtttctgta aattctttct taggtttgtg atccttttga tgacgtgggc
    40801 ggtgagggcg ctcagacttt tcaccttttt catcatgctc aggttttggc ggacgaggta
    40861 gaagagcctt catagaggca tcgatacggc ctttttcatc aatcttgata accttaacat
    40921 caacttcatc cccgatttct accaaatcct ctacacgatt ggtacgagtc caagccatct
    40981 cagagatatg aacaagggca tctgtcttat caaagaggtt aacaaaggca ccaaatttct
    41041 cgatacgaac gactttagca cggtaaactt catccacttt ggcttcacga accaaaccag
    41101 caataatttc tttggcacgg ttaatagcat cttggtcact agagtagata gacacatttc
    41161 cttcttcgtc tatatcaatc ttaacacctg tttcagcgat aatcttgtcg atggtttctc
    41221 cacccttacc gatgacaatc ttaatcttgt ccacatcaat cttgatcgta tcaattttcg
    41281 gagcagttgg agccaattct ggacgaactt ctggaatggt tgcttcaatg acatcaagga
    41341 tttcaaaacg cgctttcttg gcttgagcaa gagcctccgt caagatttct gcagtaatcc
    41401 cttgaatctt gatatccatt tgaagggctg taatcccatc acgagtacct gcaaccttga
    41461 agtccatatc tccaaagtga tcttccaaac cttggatatc tgtcaatact gtgtagttat
    41521 ttccatctga gataagtccc atagcaatac cagctactgg cgccttgatt ggcacaccac
    41581 cagccataag ggcaagagtt cccgcacaga tagaagcttg agatgaagaa ccgtttgatt
    41641 ccaaaacttc tgctactaga cggatagcgt atgggaattc ttccaagctt ggcaagactt
    41701 gagcaagagc acgctcacca agagcaccgt gaccgatttc acgacgacct ggcgcaccgt
    41761 aacgacctgt ttcccctaca gaatattgag ggaagttata gtggtgcata aagcgtttct
    41821 tgtactctgg atccaaacca tcaatgattt gagtttctcc catcggagcc aaggtcaaga
    41881 ctgaaagagc ttgagtttgc ccacgagtaa agagacctga accatgtaca cgaggaagga
    41941 agtcaacaac cgcatccaaa ggacggattt catcgacctt acgaccatca ggacgcacct
    42001 tgtcttctgt aattaaacgt cgcacttctg cgtgttccat ttgttccaag atttcagcca
    42061 catcacgcat aatacggtca aattcttcgt ggttcgcata tttttcttcg taaacggcag
    42121 tcacttggtc tttcactgct tgagttgcag cttcacgggc caatttctct tctacttgaa
    42181 ctgccttttg gaggtcactg ttgtaggctg caatgatttc agcttgcaat tcagcatcca
    42241 cgtgaagcaa ttccacttct gctttttctt taccgacagc agcaacgatt tcttcttgga
    42301 aggtaatcaa ttctttgaca gcttcgtgcc ctttaagaag cgcttccaac atgatttctt
    42361 ctgacaattc tttggcacca gactctacca tgttgatagc gtgcttggtt ccagctactg
    42421 tcaattcaag aagagattgc tctgcttgtt cttgacttgg gttgatgatg atttggccat
    42481 ctacatatcc cacttgtacc ccagcaattg gtccgtcaaa tggaatatct gaaatagaca
    42541 gtgccaaaga tgaaccaaac atagcagcca ttggtgcaga tgcattttca tcataagaaa
    42601 gcactgtatt gatgacttgg acttcattac ggaaaccttc cgcaaacata ggacgaatcg
    42661 gacggtcaat caaacgcgct gtcaaggtcg catctgttga aggacgtcct tcacgtttca
    42721 taaagccacc aggaaacttc ccagccgcat acattttttc ttcgtagttg acttggagtg
    42781 ggaagaaatc cccagttgcc attttcttag acataacggc agcagtcaag acagttgact
    42841 caccgtaacg tacgacaaca gagccatttg cttgcttagc aacctgacca gtctctacaa
    42901 ttaactcacg acccacaaaa gtcgtttgaa acacttgttt tgccatttta atcccctttg
    42961 gattgatgaa attatacgcc ttgcctacaa agatcaagat accaaggacg tcaaaagcaa
    43021 agtaaaaata ggaaactgac gaagtcttcg atgaagacaa gacagtttat cttttttaca
    43081 cagcttttcg gccgtgttca attacacaag atattttgga cggttcggct tgccgaacat
    43141 ttctgtagaa aaataggaag gtgacgtcgc actcgacgag tgctaggaag cttatctttt
    43201 ttcctaagaa atgagaccaa aattcaatta ctcaagatgc taagccgtca agcaactcaa
    43261 aggaagatag gaaatcgaac gacggagcga ctgctcctag ggagatttat ctttttccac
    43321 agagttgtag gcaagttcag ttttcaagat acatcattag aaaggtttaa tattaaagta
    43381 tctaaacctt tcacgctaat tgctatcggg cgattagcta aatgctttac taactctttc
    43441 gtcaaataac atcgatttga ctcactcgtg tcgttaaatc ttacagttta aatgcattgt
    43501 attatttaat accttcatct ttgtatcaag tacgtacaga atctatttta tcatattttt
    43561 cttaaaaagt gaggtctttg ccattaaaaa ggaaccattc ccctcacctg agaagaatgg
    43621 tttgctttta ttatcctaga gactggtgat taaacaaggc atgggttgct tgatggatgt
    43681 attttgctgt atcagcatta ttcatcgtat agagatgcac accggcaaca tcctgagtta
    43741 ccaagtccac gatttggtcc actgcatagg caagtcctgc tgctctgagc gactcagggt
    43801 catgctcata cttgtctaag atggctttaa atttgcgtgg aagatggata ttctcacaag
    43861 tcttcaagag tcggagagcc tgatttcgat tcagaattgg cataattcct gcatgaatgg
    43921 gaacatcaat cccagccaag atgcacttgt cctgaaaatc atagaagcgc tcattgtcaa
    43981 agaagagctg agttacgagg ctcgaacagc ctgcatccac tttcttctta agattttgaa
    44041 tatctgaaat ctgatttggc gaatctggat gcccttctgg atagcaagct ccaataatat
    44101 caaagtgagg ggtttgttcc ttgataaact caatcaagtc ggttgcatag cggaaatcct
    44161 tttgtggttc cacgtctgga ataatatccc cacgaagagc caagattttc tgcaccccaa
    44221 ctttgtccaa gtcagcaata gtttcagcaa ccttgtcctt agttagataa atagctggca
    44281 agtgggcaat ggtcggaatc gccaaatcat tttggataaa gtcagccaaa cgaaccgtcg
    44341 tttccttgat attaaattta ttattgctgg cagttacact gataaaatgg ggagccaact
    44401 cctgcatatc ctgcaaggct gaaataatgt taccattacc cacggctggg tttggaggga
    44461 acacttcaaa tgagagtgac ggtgtttggc gtgacatatg taataacctt ttctagttga
    44521 tttctttttg aacaaccact gtatggagag aaatccaatc ttacaatttc tcacgcgcag
    44581 ctttagctgc ttcaacaagg cggatcaagc tttcttttgt ttctgggata ccacgtgttt
    44641 tcaaaccaca gtcagggttg atccaaactt tcttgcttgg cactttagca aggatggctt
    44701 cgattgtgtt gtcgatttcg ccttcatttg gtacacgagg tgagtggata tcgtaaaccc
    44761 caggtcccac ttctgtttgg aagtttttcg ctttgagttc gtccaagatt tcaaggtttg
    44821 aacggctagc ttcaaaggaa ataacgtctg catccatgtt atcgatagct gggatgatat
    44881 ctgtaaattc tgagtaacac atgtgagtgt ggatttgtgt gtctggcgct actgttgagt
    44941 gtaccaagcg gaaggcagga atagcccagt caaggtagtc ttcgtaccag tcgctacggc
    45001 ggagtggcaa tttttcacga agagcagcct cgtcgatttg gatgattttc acaccagcag
    45061 cttcaaggtc aagtacttca tccttgatag caagggcgat ttggagagtt gaatccttga
    45121 tagagatgtc ttcacgtggg aatgaccagt taaggatggt aacaggtcca gtcaacatac
    45181 ctttaacagg tttgtttgta cgactttgtg catagctaga ccatttaaca gtgatagggt
    45241 taagacgagt gacatcaccc cagatgattg gtggttttac cccacgcata ccgtatgatt
    45301 gtacccatcc atttttagag aagaggtatc ctgacaagtt ttgaccgaag tactcaacca
    45361 tgtcattacg ctcaaattca ccgtgaacaa ggacatcaaa gtcaatatct tcttgccact
    45421 tgatccattc gtcaatcgtt tcagcaagga aagcatcgta ctctttttga gacaattcac
    45481 ctttacggta agccaaacgt ttggcacgaa cttcttttgt ttgagggaat gaaccaatcg
    45541 ttgttgttgg aagagctgga agtttgaaag cttcttcttg gatagcttca cgttctgcaa
    45601 aggctggcaa acgagtgtag tctgcgtctg tcaagccagc gatacgcgca cgaagttcag
    45661 cattttcacc aacacgctca gtcgcaaaga gttctttgtt ggctgcaaga gcttctgaac
    45721 cttgaccatt gcggatagca tccaagtcac ggatttcatc caatttttca actgcaaagg
    45781 caaagtggtt caagagtgct ggttcaaatt cttcattagc agttgtaaat ggcacatgaa
    45841 gaagtgagca agagcttgtc aaaacgatgt tttcagctgg aatttgctca agaacagcca
    45901 agctcttttc gtagttgttg cgccagatgt ttttaccatt gacaatacct acatagagag
    45961 tcttgtcagc tgggaagcca cctttaacga gttcaagagt tttcttacct tcaacaaagt
    46021 caagaccgat agcatctact ggtaagttta caaggtcagc gtatacgtca cgaacatcac
    46081 cgaaataagt ttgaagcaag acttcaagac cttttttgtc agccaagagt ttgttgtaaa
    46141 ggttcaagaa gagagctttt tcttcagctg tcaagtcttt tacaagagcc gcttcatcca
    46201 attggatgca agtcgcacca agttcagcca atttagcaaa aacttcttgg taagcagcca
    46261 ctaagctatc tacgaagtcg tctgctttca cgccttcttc aaagtctgac aattgaagga
    46321 aagtgaaggg acctacaaga actggacgag tgttcaatcc aagttctttt gcttcttgga
    46381 actcatcgaa aatcttgtga ccagccaatt tcacttgagt gtctttttca aatttaggaa
    46441 cgatgtagtg gtagttagtg ttgaaccatt tcttcattgg aagggcgcga acgtcccctt
    46501 tttctccctg gtaaccacgt cccaaagcga agtagcgctc aaggtcagac aagtccaagt
    46561 tttgaacaga tgcaggcacc acgttgaaaa ggaaagccgc atctaggaag ttatcatagt
    46621 gagaaaagtc atttgatgga atttcagtga tgcctttttc tttgacaatg ttccagtgtt
    46681 tagcacgcaa gtcttttgct gctgctaaaa gttcttcttc tgagatttct tttctaaagt
    46741 atttttcagt tgtaaatttt aattcacgga attcgcccaa acgagggaaa ccgatgattg
    46801 tagttgacat gatgtgtcct ccaaaatttg ttgttgaaac tatcttaaca gaaaagaaag
    46861 cgtctgtata attgtaaaaa attagggttt gatatagttt gaaactatat atctgtttcg
    46921 gacaaaagaa aaagacttga agcaaacgtc tcaaatcctt tgtatagctt gttttatagc
    46981 tgtattttag ttagaatgct caaacgcgtt attagtaatt cttataagtg actatggctt
    47041 gttattagaa aagactataa ctgattctag tcaacttttt ccctgttcaa gtgggacgat
    47101 tgctagtgtc tttcctaaac tggctaggac ttttaagact gtatccaact gaggactagt
    47161 ctttcctgtc tccatcctag ctatgacagg ctggcttatt ccactgactt cttccagctt
    47221 tttctgactg attccttgtt catacctagc ctcaatcaac tcgctcatga tagccactcg
    47281 catatcactt tcaaggattt cctccttgct aaagagctca gatggacatc cttccaatta
    47341 ctcccaatag cactattctt catcacttaa ccctcttttt tttacgtcta tgtattttta
    47401 aaaaaatgag cgaattatga ttcgatagat tgaccagcgg gtttaaagtt ggtgctagcc
    47461 tatttcttaa gcgattttcc ttttctagga taaagcagat cctgcttgct taaccccaat
    47521 tttccacgat gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc
    47581 accatcattt caggcgaaaa tttttgatta tgtatagtgg agaatctttt cctttagttc
    47641 cttggtcaag cttgatttct tgaccgagcg cttgcgattg ttttcataag actgttgagc
    47701 gtagtcggca gaataaacct ctttgaagcg cccttttcca agacattgtc ggactgtccc
    47761 acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg caacttctct
    47821 atttgatttt ccttcttttt tccatctttc gattaagcga cggctatcga ttgtcaaatg
    47881 tttacctttt gtagtataat tgtcttgcat ctctgtgcct tttaatcatt tcaatcttaa
    47941 attggacttt ttttacttgg gttgtactta atctataagg aagacaataa aaagaatatc
    48001 aatcaagtaa agtcacaaag tcacattagc tccgagcaac cattgcaaat tgaggtactc
    48061 acacaatgat taaaacattt ctctctgccc tttcggtcat tctctttgtt atccctatca
    48121 tagcttattc tttttttcca tcttctaatc ttaatatttg gctatctacc agacctatct
    48181 tggcacagat ttatgccttc cccttagcta ctgcaactat ggcttctatt ttaagtttcg
    48241 tatttttttc cctatctttt tacaagaaaa ataaacaaat acggttttac tctggcattt
    48301 tgctcttact atcgctcata ttactattat tcggaacaga taaaaccctt tcttctgcat
    48361 caaataagac taaaacctta aaattagtaa cttggaacgt cgctaatcaa atagaagcac
    48421 aacatattga gcgaattttt agccattttg atgccgatat ggctatattc cctgaactag
    48481 ctaccaatat cagaggtgag caagaaaacc agagaatcaa actattgttt catcaagttg
    48541 gactttctat ggccaactat gatattttca cttctccacc taccaatagt ggaatagctc
    48601 ctgtgactgt gattgtcaag aaaagttatg gtttctatac agaagctaaa acttttcata
    48661 caacacggtt tgggacaatt gtattacatt cgagaaaaca aaatatacca gatatcattg
    48721 ccttgcatac tgctcctcct ctgccaggtt taatggaaat ctggaagcaa gacttaaaca
    48781 tcattcataa tcaattggct tcaaaatatc caaaggctat tattgcaggt gattttaatg
    48841 caactatgcg tcatggagca cttgcaaaaa taagctctca tagggacgca ttaaatgtac
    48901 tgccaccttt tgaaagagga acttggaata gccaaagtcc aaaacttttt aatgcaacaa
    48961 tagatcatat tttattgcct aaaaaccact actatgttaa agatttagac attgtaagtt
    49021 ttcaaaactc tgatcataga tgtattttta cagaaatcac attttaatta ttttatataa
    49081 aatcacccct ctagtgttca taaactagag ggggaatttg tatcatacta tcgtttaacg
    49141 cacttctgca ttcacttttt cttcgagcga tgcttggatt ttttccatat agcgtgcgac
    49201 ttcttcgtcc gttaagctat cttctggatt ttggaaggtc aagctataag ccattgactt
    49261 cataccaagt cccaatttct cacctgagaa gacgtcaaag agtttgatat ctgtcaaacg
    49321 tttcacgccg gcagcttgga tagcatctac aacttcttga tgagtcactt ctgccttgag
    49381 gagaagggca acgtcacggc tgactgctgg gaatttcgtg atttccacaa atggaacagc
    49441 aggttggagc gccccttcga tggctgaaag gttaagctca gctacatacg tttctggaat
    49501 atcgtaagcc ttggcagtga ctggatgcac ttggccaagg aaaccaagaa cttggtcacc
    49561 gagtgaaatc acggctgtac gacctggatg aaggctagcg atttcagatg ttgctgtata
    49621 ggttacttgg agtcccaaac gagtaaatag ggcttcaagg attcccttag catagaagaa
    49681 atcaactgga actgctgctg tttggaaatc tttttcagca accaagcctg tcaaggcaaa
    49741 ggcaaaactg ttgatttcat ttggaagttc ttcttttgga ttacctgttt gttcaaagac
    49801 ttttccaatc tcgtaaagag ccaagttttt attcttacga gccacgttgt aggcaacggt
    49861 atcaaggata cctgaaatca tattttgacg gaggactgaa cgatccacag tcattggcca
    49921 catgagttca gtaaggttac ttggttgagc cgtaaactca actgcttttt caggagttgt
    49981 cagagcatag gtgatgattt ctgtcaaacc tgctccttca gcaatagtac gaacttgacg
    50041 gcggagtttt tgtgtggctg tcaattcacc tgctgtacca tcgtcttttg gtagactagt
    50101 tggcaagcgg tcataaccat agatacgagc aatttcttca aaaaggtcag cttcgattgt
    50161 gatatcccaa cgacgacgtg ggactctgac tgtaaagctg tctgcatttc cagaaagacc
    50221 aaagccaaga cgacggaaga cgtcttctac atcagcataa gacagctcag ttccgaggac
    50281 acggttaaca tcagcaaggg ttgaagaaac ttctacatct gaagtatcaa gctcacccgc
    50341 tgaaacgatg cccttacgca ccgtcgcacc tgcaagttcc gcaatcaggc tagatgccgc
    50401 atcaagggct tcattaactg ttgccacatt aattcctttt tcaaagcgag aagatgactc
    50461 agaacgaagg ttcaggcgac cacttgtctt acggatagat ttgccattga aaacagcagc
    50521 ttcaaggaca acacgactag atttttcaga gatttctgtt gcttgaccac ccatgacacc
    50581 tgcaagggct actggcttgt ctgcgacagt gatgactagg tcattcacgt ccaagtcacg
    50641 ttcttcaccg tccaaggtca ccaatttttc accagcacgc gcttcacgca cacggatgtc
    50701 agtcccttca aagttatcca agtcaaaggc atgcattggt tgaccaaaat agagcaggat
    50761 gtagttggtc acgtccacta cgttattgat gggacggatg ccttcgttca tgagaaggtt
    50821 ttgcaaccat tgtggacttg gtgcgatggt cacattttcc aagatacgag ctgcatagta
    50881 aggcgccttg tctgtctcaa tgctgacaga aagggcatct gccgcagctt cattagtttc
    50941 tgttagagta aattctttaa agttgactgc cttgtcatag atggctgcca cttcgtgagc
    51001 cactccacac atagaaaggg catctgcacg gtttggtgtg atggaaagtt cgatgatttc
    51061 atcatccaag tctaggtaag aaaagacttc ctcacctggc acggcatctt caggcaagat
    51121 ttggatgcca tctgcgaatt ccttaggcac aactgagtca gaaatcccca attcaccaag
    51181 tgaacagatc attccaagtg actccaaacc acggattttt ccttttttga ttttgtagtt
    51241 atcagcgata cgagctcctg gaagagccac catgaccttg atcccagcac gcacatttgg
    51301 ggcaccacaa acgatctgac gggcttcttc ttcgccaacg ttaacctgac aaacatggag
    51361 gtgagtctct ggcacatctt cgcaagacaa gacctcaccg acaacaattt ttgagagatc
    51421 agcagctggt gattcgacac tctctacctc gatccctgtg gttgacattt tttcagccaa
    51481 ctcctgtgat ggcacatcaa tgtccaccaa ttcttttaac catttataag atacaagcat
    51541 aatttagttc tccagaatga ccattgccac actggttctt ttcctttcct atcatttcaa
    51601 tagaagaatc ctcttcttac cttaatttct ttctcagtaa ccaatccgta tctacttttt
    51661 gaccaaccat aaaatgatgt tggctaaatt tttcaaaacc atatcgatta taaaacgctt
    51721 gagcttttgt attatgctcc caaacaccta gccaagccca agaaaaacta ttttttgtag
    51781 caagttcaag tgcgaattca aacagttgct tacctattcc aaatccttgg aatttttgta
    51841 gcacatagag gcgttgaatt tcaaaagcgt cctctaattc tctctcagtt tgagcacttc
    51901 cccagttgac tttgagaaaa ccagctatct cctcctcatg cataatgaaa taggtttcag
    51961 agtcaggatt tcccaactca gttgacaaag ttttcagact ataagcctct tcaaagtatt
    52021 cctgtaactg ctcttccgta ttatcatacg caaaggtttc acgaaaggct tgtttggcaa
    52081 ttttagccaa cacctcaaca tctgccattt ctacttttct aatcattatt taaactgttc
    52141 tgagaagcgg acatctcctt ggtagaatcc acggatatcg ttgattccat aacggagcat
    52201 agctacacgc tcttgtccaa gaccaaaggc aaaaccagag tatacagtcg catcgatacc
    52261 actcatttca aggacacgtg ggtgaaccat accggctccc ataatttcga tccaacctgt
    52321 tttcttacat acgttacagc cttctccacc acacttgaag caagaaacat ccacctcaac
    52381 agatggctct gtgaatggga agtaagatgg acgcaaacga atttgacgct cttcaccaaa
    52441 cattttttgg acaatcaact gaagcgttcc ttgaagatca gccatagaga tatttttccc
    52501 aactaccaag ccttcgattt ggtggaattg gtgactgtgg gtcgcatcgt ccgtatcgcg
    52561 acggaagaca cgccctggcg agatcatctt caaaggacct ttagaaaaat catgggcatc
    52621 catagcacgc gcctgaactg gagacgtgtg ggtacggagc aagatttctt cagtgatata
    52681 gaaagtatcc tgcatatcac gagctgggtg gtcttttgga aggttcatac gttcaaagtt
    52741 atagtagtct tgctctactt caaaaccatc cacgacttga taacccatac cgatgaagat
    52801 atcttcgatt tcttcactgg tttgtgtcaa aacgtgacgg tgaccagtcg caactggacg
    52861 acctggaagc gtcacatcga tactctcgct agccagttga gccgcgactt tcttttcttc
    52921 caagagctta gctgtttctt caaaagcagc agtcaagaca tcacgagctt cattgacgtg
    52981 tttcccgatg attggacgca tctcagcaga aacatctttc atccctttga ggatttcagt
    53041 gagcgaaccc tttttaccaa ggacagagac acgcaaatct tgcatctctt tttcatttcc
    53101 agcagtaatc tgcttcaagc tagccagcgt ttcttcgcga agcgctttta attgttcttc
    53161 aatagttgac atatttcctc catcagtctc tcgtagataa aaagaaaacc acatgccaaa
    53221 aactccactc ggagcgttga cacgcggtac catccgtttt catctgacaa gtcagacctt
    53281 catttctaaa tccatgcgca agtgaattca cccagctttc atatagagag cttgcagtca
    53341 cggctctcct ccctgatata cttcccttgg gctactagtc tttcagattc ctattcaatt
    53401 actacttagt ttatcagatt tttaccattc ttgcaagacc tatcttactt ctgcttgtta
    53461 gcttattctt atctaacttt atagcggatt gaaataagat gagaacaaat cgattgggaa
    53521 agtaaaatta atttctataa atgttttagc aattgtttcg tactatttta gattcagtct
    53581 actatatata accaatctat cctaagttaa ctatttataa tcggctctct gtaacaaaat
    53641 taaattaatc cccctataaa ataaagaggt ttagaatttg atgtcttcca aacctcttta
    53701 tttcatattt aatgagatgc tgccttaacc gtgataatat ctaatcatca ataaacaaac
    53761 tatttgaata aggattctcc atttgattca atcactcctt tataccaagt aaaggacatt
    53821 ttcttatatc gattgaatgt gccatttcca tcatcatttc gatcaacata aatgagaccg
    53881 taccttttag aaagttgtgc agtggacata gaaacacagt caatacatcc ccaagacgta
    53941 tagcccataa tttcaacacc atcctgtaga gcttcagcaa cttgcaataa atgttctttc
    54001 atatactgaa ttctataatc atcttggacg gttaagttat taagttcatc ttttattagt
    54061 tgatctttag cacctaatcc attttctact ataaataatg ggatttgata acggtcataa
    54121 tatctattta aaattatacg tagtccaatt ggatcaattt gccatcccca ctctgaagac
    54181 tctaaataag gatttactaa accaccaata atattccctt ctcctgaatt atactgtgtt
    54241 ggaagagcag attgagtcac actcatgtaa tagctaaagg ataaaaaatc tacagtataa
    54301 ttttttaata actctgcatc ttcagctgca aactctatgt taatgtcatt ttccttaaaa
    54361 tatctttttg cataattcgg ataataacct ctaacatgca catctgaaaa tagataattt
    54421 agattctcat actcatgagt cgcccataca tcttttggat ttggagtcat tggataagct
    54481 ggcatagcta ataccataca ccccacctta aactctgaat taatctcacg agcaattttt
    54541 gtaaccaaac tcgaggcgac taattcatga tgtatagctt gatataattc ttgtttcgaa
    54601 agattctcct taggtatatc tattcctcca ctagtaaacg gtaattccaa aacagagttt
    54661 acttcgttaa atgtaagcca atatttaact ttatctttat acctttctaa aactgttcga
    54721 gcaaattttt cataaaaatg aatcattctc ctatcaatcc atccatgata ttttcttgct
    54781 aaatataatg gagtctcata gtgtgaaaga gttacaagtg gttctatccc gtgagcatgt
    54841 agttcatcaa acaattcatc ataatatttc aacccagctt cgttaggttc ttcctcatct
    54901 ccttttggaa aaattctact ccatgcaata gaagtacgaa aaacattaaa gcccatttca
    54961 gaaaacaagg ttatatcttc cttatattta tgataaaaat caatacctat caattttaag
    55021 ttatcttctg taggattttc tgttgcttct cctaatccac ctttgggtaa cacatcctga
    55081 actgataagc ccttaccatc ttcattatat gctccctcta cttgattagc tgcaacagct
    55141 ccaccccaaa gaaaatcatc tggaaaaatg gtcataactt tcctccatta taatattacc
    55201 agtaattcct tagaatgctc gattgtctga ttattaggta atactaatac atctagaaaa
    55261 tcattggtat tcgttacaat tactggtgta actgtttcgt agcctttagt cttgattaaa
    55321 ttcaagtcca tttcaaaaat caactgattt ttgaaaactc tgtctccttc ttctacatga
    55381 ctaataaaac cttgaccttt tagctcaaca gtatctaatc caatatgaat tagtaactca
    55441 ataccctcat cactcttcaa tccaattgcg tgcttagtcg gaaaaatatt tgtaattttc
    55501 ccatcaaatg gtgcataaac cttaccttca cttgggataa tcgctactcc gtctccaatt
    55561 agtttatctg aaaatgtttt atcctggaca tcgcttaacg gaatgatttc tcctgatata
    55621 ggagaaaata tcattttttt atttgaaact ccagctttaa cttctaaatt gctagaactc
    55681 tcttcttcat cgattccaaa tatataagct aatacaaagg taataacaac cgaaatgacc
    55741 gccacaatta aagcatttac aatatttgat ggcacatcag aataaataaa ttgaggcaac
    55801 gctatcaaag atgggacagc aaatagatac gctttaacac tagtaagacc tgcaaataat
    55861 cccgctaatc caccaccaat catagctgca taaagcggtt ttttatattt taaagtcaca
    55921 ccatatactg caggttcggt aatccctgca agtaaggctg agaaacttgc tgcaaaagca
    55981 atttgttttg tattattatt tttactcttt aatgcaacag ccatcgaagc agccccttga
    56041 gctaagtttg accctaacat tgctggaaga attaatacgt ctggagtagc aatagatgcc
    56101 gccaaaaaaa taggtgcaaa agcccaatgc attccagtca taacaataaa tggcataata
    56161 gcaccaagaa tagctaatgt aagccatcca gctacaccat acatttgccc aactagattt
    56221 gataatcctt caccaacaat tactccaata ggtccgacta caactaaggc aatacagctt
    56281 gatactaata atactagcgt aggttgcaaa aaactcttag taatagctgg tgttaattta
    56341 gcaattattt tttcaatata tttcatcaac caaaccataa taagaattgg aacgactgat
    56401 gaaccataac tagctggtgt cacaggtgca ccaaataaac taagaggatt ccctgattgc
    56461 accatttgaa caaaatttgg atggagaagt acacctgcta cagacatagc taatgtagat
    56521 gttactttta atttttgtga tgcagaataa gctaataaca gcggtaagaa ataatatgga
    56581 gcatccccaa aaaatgtcaa aaaagcaata gtctgagaat ctgattgcaa tataccaagc
    56641 attggtaaaa tgattaccaa gactttcaac atacctcccc ctaacattgc tggaatgatt
    56701 ggagtcatgg aaccagcgat atactcaatg attctttcta aaatattccc tttgtgccct
    56761 tgaacaactg aatcggattc aaaattgcca agtttaacga attctttata ataattagct
    56821 acatcattac caagtataat ttgatattgt ccattctttt tcataatacc tattacacct
    56881 ggtatcttct tcacatcatc atcattgact aaattttcat cttttaattc taatcttaaa
    56941 cgtgttacac aatgggtaac tctattgaca tttttttcac ctccaattac atcgaggatt
    57001 ttttgtaccg tatctttata actcatggta ttctcctatt ctattaatct aaattttttg
    57061 ttaagcgacg aatatgagcc atcaaataaa ctaattcact agaagtcagc aaataattgt
    57121 actccgtttg tataaacatt gccacctgtt caccacattc atattctcta ggatatttat
    57181 ttttcattaa tgctaacaag tcttcatcat catcgtcaca ataagttgta ttttcaacca
    57241 agcgatgggc aaataatttt atatgagtta tgaatctata ataatcaata gaatcttggt
    57301 taaactcagt acaatagaaa tcttgaatga ttttctcaat tcccataatt atttcagtga
    57361 ttttatacga ctcttgaaaa ttattttcta aattagcatt aacaaaatgt agcgctacga
    57421 atgcagcttc gtcaattgga agttcaatac ctaactcttt ttttattatt tgtaaagccc
    57481 ttttcccaag ctcaaattct tctttataat atttcgagat ttcccagcgt aggggattcg
    57541 aaataataac tccttcttta taacgttcta tactcgaaga aatatgatcc gttagattta
    57601 tatagataat ctcatcaaaa ttattaccta atgttatttt acctagatta attatttttt
    57661 cggtgatgta caccaactcc aaaggaacgt taataaacat ctctgtaaaa cgggtcatat
    57721 tatctctatt tttgagaaaa aaacttttct caatatcaat tggatttact ttatctccaa
    57781 tttttcttcc aaaagctatg ccccttccaa aaagaagaat atcgatatct tttttacttt
    57841 gcgcaattac ggcattgtgg tttagtattc gttttatgat cataaaattt cccttttaaa
    57901 ataaaaggaa cctacttaaa aaacatcaag aaacttcttc tgatgtgatt aaataggttc
    57961 ctgctcactt gtattagtga taacatcctt ttatttcaat ttttaatggt gacatgaagc
    58021 tta
//
LOCUS       DBERCA010000008        52269 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.008, whole genome shotgun sequence.
ACCESSION   DBERCA010000008 DBERCA010000000
VERSION     DBERCA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 52269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.008"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(394..1590)
                     /gene="tuf"
                     /locus_tag="ACL719_000541"
     CDS             complement(394..1590)
                     /gene="tuf"
                     /locus_tag="ACL719_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS0698559.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2501..3370)
                     /gene="gla"
                     /locus_tag="ACL719_000542"
     CDS             complement(2501..3370)
                     /gene="gla"
                     /locus_tag="ACL719_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGS0698560.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3593..4114)
                     /locus_tag="ACL719_000543"
     CDS             complement(3593..4114)
                     /locus_tag="ACL719_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS0698561.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(4072..5178)
                     /locus_tag="ACL719_000544"
     CDS             complement(4072..5178)
                     /locus_tag="ACL719_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGS0698562.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5289..5543)
                     /locus_tag="ACL719_000545"
     CDS             complement(5289..5543)
                     /locus_tag="ACL719_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698563.1"
                     /translation="MLNVDQDFMSISKSNKSDSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5537..5779)
                     /locus_tag="ACL719_000546"
     CDS             complement(5537..5779)
                     /locus_tag="ACL719_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698564.1"
                     /translation="MYQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(5901..6434)
                     /locus_tag="ACL719_000547"
     CDS             complement(5901..6434)
                     /locus_tag="ACL719_000547"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS0698565.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6421..6747)
                     /locus_tag="ACL719_000548"
     CDS             complement(6421..6747)
                     /locus_tag="ACL719_000548"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS0698566.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            6981..8699
                     /locus_tag="ACL719_000549"
     CDS             6981..8699
                     /locus_tag="ACL719_000549"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGS0698567.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAILVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8810..9157
                     /locus_tag="ACL719_000550"
     CDS             8810..9157
                     /locus_tag="ACL719_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS0698568.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9435..10271)
                     /locus_tag="ACL719_000551"
     CDS             complement(9435..10271)
                     /locus_tag="ACL719_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698569.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10284..10913)
                     /locus_tag="ACL719_000552"
     CDS             complement(10284..10913)
                     /locus_tag="ACL719_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0698570.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10923..11564)
                     /locus_tag="ACL719_000553"
     CDS             complement(10923..11564)
                     /locus_tag="ACL719_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0698571.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11710..12939)
                     /locus_tag="ACL719_000554"
     CDS             complement(11710..12939)
                     /locus_tag="ACL719_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS0698572.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEESENLLT
                     KWMIARSYQEMDDLDTAYEHYQELIGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12929..14095)
                     /locus_tag="ACL719_000555"
     CDS             complement(12929..14095)
                     /locus_tag="ACL719_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGS0698573.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14239..15252
                     /locus_tag="ACL719_000556"
     CDS             14239..15252
                     /locus_tag="ACL719_000556"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGS0698574.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15297..15716)
                     /locus_tag="ACL719_000557"
     CDS             complement(15297..15716)
                     /locus_tag="ACL719_000557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGS0698575.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15727..17133)
                     /gene="atpD"
                     /locus_tag="ACL719_000558"
     CDS             complement(15727..17133)
                     /gene="atpD"
                     /locus_tag="ACL719_000558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGS0698576.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17219..18097)
                     /locus_tag="ACL719_000559"
     CDS             complement(17219..18097)
                     /locus_tag="ACL719_000559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGS0698577.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18113..19618)
                     /gene="atpA"
                     /locus_tag="ACL719_000560"
     CDS             complement(18113..19618)
                     /gene="atpA"
                     /locus_tag="ACL719_000560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGS0698578.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19633..20169)
                     /locus_tag="ACL719_000561"
     CDS             complement(19633..20169)
                     /locus_tag="ACL719_000561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGS0698579.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20169..20663)
                     /gene="atpF"
                     /locus_tag="ACL719_000562"
     CDS             complement(20169..20663)
                     /gene="atpF"
                     /locus_tag="ACL719_000562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGS0698580.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20677..21393)
                     /gene="atpB"
                     /locus_tag="ACL719_000563"
     CDS             complement(20677..21393)
                     /gene="atpB"
                     /locus_tag="ACL719_000563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGS0698581.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21428..21628)
                     /locus_tag="ACL719_000564"
     CDS             complement(21428..21628)
                     /locus_tag="ACL719_000564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGS0698582.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21703..22638)
                     /locus_tag="ACL719_000565"
                     /pseudo
     CDS             complement(21703..22638)
                     /locus_tag="ACL719_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23087..23485)
                     /locus_tag="ACL719_000566"
     CDS             complement(23087..23485)
                     /locus_tag="ACL719_000566"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698583.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(23858..24340)
                     /gene="greA"
                     /locus_tag="ACL719_000567"
     CDS             complement(23858..24340)
                     /gene="greA"
                     /locus_tag="ACL719_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGS0698584.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(24411..26066)
                     /gene="mltG"
                     /locus_tag="ACL719_000568"
     CDS             complement(24411..26066)
                     /gene="mltG"
                     /locus_tag="ACL719_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGS0698585.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTINELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTDIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(26147..26641)
                     /locus_tag="ACL719_000569"
     CDS             complement(26147..26641)
                     /locus_tag="ACL719_000569"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698586.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(26638..27102)
                     /locus_tag="ACL719_000570"
     CDS             complement(26638..27102)
                     /locus_tag="ACL719_000570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698587.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDCKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(27112..28446)
                     /gene="murC"
                     /locus_tag="ACL719_000571"
     CDS             complement(27112..28446)
                     /gene="murC"
                     /locus_tag="ACL719_000571"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGS0698588.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(28457..29074)
                     /locus_tag="ACL719_000572"
     CDS             complement(28457..29074)
                     /locus_tag="ACL719_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS0698589.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(29122..32220)
                     /locus_tag="ACL719_000573"
     CDS             complement(29122..32220)
                     /locus_tag="ACL719_000573"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0698590.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQVLVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(32313..33479)
                     /locus_tag="ACL719_000574"
     CDS             complement(32313..33479)
                     /locus_tag="ACL719_000574"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGS0698591.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQIYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(33487..34581)
                     /locus_tag="ACL719_000575"
     CDS             complement(33487..34581)
                     /locus_tag="ACL719_000575"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS0698592.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(34738..35325)
                     /locus_tag="ACL719_000576"
     CDS             complement(34738..35325)
                     /locus_tag="ACL719_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698593.1"
                     /translation="MLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSG
                     SIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRL
                     QIARTYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDC
                     IFFLEEGKITGSGTHKELLENHERYACFVQEQMIE"
     gene            complement(35319..36232)
                     /locus_tag="ACL719_000577"
                     /pseudo
     CDS             complement(35319..36232)
                     /locus_tag="ACL719_000577"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(36201..36401)
                     /locus_tag="ACL719_000578"
     CDS             complement(36201..36401)
                     /locus_tag="ACL719_000578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698594.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSYWSSLVFFYWLLSV"
     gene            complement(36573..38531)
                     /locus_tag="ACL719_000579"
     CDS             complement(36573..38531)
                     /locus_tag="ACL719_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0698595.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     AYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(38734..40356)
                     /locus_tag="ACL719_000580"
     CDS             complement(38734..40356)
                     /locus_tag="ACL719_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS0698596.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            40462..41907
                     /locus_tag="ACL719_000581"
     CDS             40462..41907
                     /locus_tag="ACL719_000581"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGS0698597.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLREQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISNYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(41950..42147)
                     /locus_tag="ACL719_000582"
     CDS             complement(41950..42147)
                     /locus_tag="ACL719_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS0698598.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(42248..42897)
                     /locus_tag="ACL719_000583"
                     /pseudo
     CDS             complement(42248..42897)
                     /locus_tag="ACL719_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(42881..43393)
                     /locus_tag="ACL719_000584"
     CDS             complement(42881..43393)
                     /locus_tag="ACL719_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGS0698599.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(43479..44414)
                     /locus_tag="ACL719_000585"
     CDS             complement(43479..44414)
                     /locus_tag="ACL719_000585"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGS0698600.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(44485..44784)
                     /locus_tag="ACL719_000586"
     CDS             complement(44485..44784)
                     /locus_tag="ACL719_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGS0698601.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGRGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(44774..45523)
                     /locus_tag="ACL719_000587"
     CDS             complement(44774..45523)
                     /locus_tag="ACL719_000587"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGS0698602.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(45577..45936)
                     /locus_tag="ACL719_000588"
     CDS             complement(45577..45936)
                     /locus_tag="ACL719_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGS0698603.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(45938..47338)
                     /locus_tag="ACL719_000589"
     CDS             complement(45938..47338)
                     /locus_tag="ACL719_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGS0698604.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILIGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(47633..47872)
                     /gene="rpsR"
                     /locus_tag="ACL719_000590"
     CDS             complement(47633..47872)
                     /gene="rpsR"
                     /locus_tag="ACL719_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGS0698605.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(47904..48374)
                     /gene="ssbA"
                     /locus_tag="ACL719_000591"
     CDS             complement(47904..48374)
                     /gene="ssbA"
                     /locus_tag="ACL719_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGS0698606.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(48386..48676)
                     /gene="rpsF"
                     /locus_tag="ACL719_000592"
     CDS             complement(48386..48676)
                     /gene="rpsF"
                     /locus_tag="ACL719_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGS0698607.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(48829..50175)
                     /gene="asnS"
                     /locus_tag="ACL719_000593"
     CDS             complement(48829..50175)
                     /gene="asnS"
                     /locus_tag="ACL719_000593"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGS0698608.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(50191..50556)
                     /locus_tag="ACL719_000594"
     CDS             complement(50191..50556)
                     /locus_tag="ACL719_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0698609.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(50549..51736)
                     /locus_tag="ACL719_000595"
     CDS             complement(50549..51736)
                     /locus_tag="ACL719_000595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0698610.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(51733..52221)
                     /locus_tag="ACL719_000596"
     CDS             complement(51733..52221)
                     /locus_tag="ACL719_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGS0698611.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEKADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
BASE COUNT        15296 a        11521 c         9617 g        15835 t
ORIGIN      
        1 ttgatttcat attactattg tataatgctt tttgatttta gtctagtatt atactcttcg
       61 aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata gatgttactg acttcgtcag
      121 ttctatctac aacctaaaaa cagtgttttg agcagtctgt agctaacttt ctatagtaaa
      181 atgaaataag aacaagacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
      241 gaagtaaatg tgtactattc tagtttcaat atattatagt ttgctctttg attttcattg
      301 agtattagtt caatatctaa aaaaagaacc ttgccaagca agattctttt agtgtctgac
      361 ttaaataatt gttcttctgg gaactaaatc gaattaagct tcgatttctg taaccatacc
      421 tgaaccaaca gtacgtccac cctcacggat agagaatgta gtaccttgtt ctacggcgat
      481 tgggtgaatc aactcaacgt cgattgtcac gttatcacca ggcattacca tttcagtacc
      541 tgctggaagt tcgattgaac ctgtaacgtc agtagtacgg aagtagaatt gtggacggta
      601 gttgttgaag aatggagtgt gacgtccacc ttcttcttta gtaaggatgt agacttcacc
      661 tttgaattta gtgtgtgggt tgattgaacc tggtttagcg ataacttgtc cacgttcgat
      721 ttcatcacgt tgaacaccac gaagaaggac acctacgtta tctccagcaa gaccttcgtc
      781 aagttgttta cggaacattt caacaccagt aacaactgct ttttgagttt cttctttgat
      841 accaacgatt tcgatttcgt cgttgacttt aacgatacca cggtcgatac gtcctgaagc
      901 aactgtacca cgtccagtga ttgagaatac gtcctcgact ggaagaagca atggtttgtc
      961 agtgtcacgt tctggttctg ggatatactc atcaactgtg ttcatcaatt ccataacgat
     1021 gtcttcgtat ttagagtcac cttcaagagc tttaagtgct gaaccttgga taactggaag
     1081 atcgtcacct gggaagtcgt attctgacaa taggtcacgg atttccattt caaccaattc
     1141 aagcaattct tcgtcgtcaa ccaagtcaac tttgttcatg aagacgataa ggtgtttaac
     1201 accaacctga cgtgaaagaa ggatgtgctc acgagtttgt ggcattggtc cgtcagttga
     1261 agctactaca aggatagctc cgtccatttg agcagcacca gtgatcatgt ttttaacgta
     1321 gtccgcgtgt cctggagcgt cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac
     1381 gtgcgcagtg ttgatagtga taccgcgttc gcgttcttct ggagcagcat cgatagacgc
     1441 atagtcttta ggttggttaa ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc
     1501 tgcagttagg gtagttttac cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg
     1561 tttactacga tcgtattttt cttttgccat ttgagtaaaa gcctccaata aaatatattt
     1621 tatagataga cagtaggcaa tacagtctaa ctttccttac tattttatca aatttcagct
     1681 aaattgcaag tgttttacaa cgtttttgtg aattattctt gataaaaatt taactaacta
     1741 atagaaagta ctaaatagac ttatccttca aggagttttc agcattatcc atccactcac
     1801 ttgctgaaaa tgtaaacagt ctctggttta tatagtaaaa tgaaacaaga atagggcaaa
     1861 tcgatcagga cagtcaaatc gatttctaac aatgttttag aagttgaggt gtactattct
     1921 agtttcaatc tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt
     1981 agtttgccct ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg
     2041 actcagcaga gtcaatgtag gtcgaagaat agccctccta atcgcttatt ttatagcaaa
     2101 atgaaataga aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag
     2161 aaagaaggga ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa
     2221 cccatctccg ataatatata gtagaatgaa aactgcacaa tttgattgga gaattcaaac
     2281 caatttctaa caatgtttta gaaacaaggg tctattattt caactgcaac ctactatagc
     2341 ccattgaagc tggaatagcc cactgagact tctaaaccac tgctggagag gagtttgttt
     2401 agagtttctt tcatttccct aaaaaatcaa aaagagagaa ccagtctgat cctctcttac
     2461 ttatagatgg tgctcactca aatgttaaag gagctatttc ttagagataa aggaatttga
     2521 atacagctac tgccgcaatt gctgctgcga taggtgctac tactggtacc caagaatacc
     2581 accattttga atcgccttta tgctcaccaa gaactgattt tggaaggaaa gcatgaagga
     2641 gacgtggtcc caagtcacgg gctgggttca aggcaggtcc tgtaggtcct ccaagtgatg
     2701 ttaccaaagc cataacgagg aatccaagtg ccaagtgagc cacagaaagt cctgaagcag
     2761 tgtgtggagc cacctgtgct ttaatagcca agtcagaaaa atcaactgtt tgtcctgctt
     2821 ctattgcctt ttgtttcatg aattgaagca cttcagcacc aaagaagttt ttagtcaaac
     2881 caagagctgc aaagaacaaa acaaatgaac caacaaactc attaatcaaa ccattgacag
     2941 ttgctgcata gcgactttct tttgtaccat ggtcaatact tgaaatagtt gagaaagttc
     3001 ccaagatgtt atttggtttt tcagttttca agtagaatgg acggtatgtt gccacaacta
     3061 aggcttggcc aaagatagcc cccaagactt gcgcgataat gtaaggtacc acttgtgccc
     3121 aagggaaaag accgctaact gcaagcccta gagtgaaagc agggttgatg tgattcccag
     3181 atacgttacc aaacatcaag gctgggatca taacccccat accataacca acagcgatga
     3241 cgatccagcc actttggtga cctttcgtac ctttaagttc aacgttggca actgcaccat
     3301 tcccaagaat gatcaaaatg gcagttccca aaaattcagt ggcatacttc agtgcccatg
     3361 tgaaatccat ttgctagatt ctccttaaaa ttttttagat attccttatt ttatcaattt
     3421 ttaccctatt tagcaactga tttctggaaa tatttttaaa aaaataggat tcatagtttc
     3481 ccagttcaac gtacttgtaa gcgtttttat tcttcacgaa agacctcatt ctcaaaacca
     3541 ggaaaaagcc cacatctgtg atcttttcct tgttcctaga ttctaaaatc tattattttt
     3601 catcttcttt tttcttgaag aatcctaatc ctagaccagc taacaaggtc atgataccag
     3661 cactagccaa ggtagcatta gcttctgtac ctgtgtttgg caattcctct ctcttacctg
     3721 tctcataagt cggaacttct gggtctggat tcactggagt ttcagttttt ggagtacctg
     3781 ggtctggagt tggtttatct ggtgttgata aacggtcata ccttaccgtt atttctttat
     3841 cactagagtc tgacgtaact tcttgtgatt caactgttgg aatatctgga tctttgtact
     3901 tgtcaatctt accagataca acctcgtccc agtttcctgt tgtccattca ccgtaggtta
     3961 caactcccgt gaccttgttc tcagtttttg tacggcttaa ggttacaggt tgaacaacat
     4021 cttcttttac attttggttc gtaactttat caacgtaatg aatgatacgc gttatagtct
     4081 tcgtctcagt agaggttgct gttttgggaa ccactgtttc ctcaacattc tcacggtagt
     4141 aatagtcaac tgttgcaccg tcttctggta cgcatttgca ggagttgcta ccaaggtgta
     4201 tgttgttttc cttgtgataa ctcggtcttc tttgtcctca gttgttgttt tcccttcaat
     4261 agtttttgat tctgtggtat actcagaacc tatcgctaaa tcagctttta taacagactc
     4321 tgccaacttc tcttggctac cttctttata gtaattggct gttactgtag cagtggttgg
     4381 cgcttcgctt tctctataaa ctaaggtcac tgttctacct tcgcttacaa tattcccagt
     4441 taaacttgca gaatttgtat ctgcttcttt aaaagtataa tattttccgt cagtagtagt
     4501 catgctactg agttttttat ctgtgacatt gtagctggta ccaatcagtt gttttttatt
     4561 ggtaatgtag gttccgtcac tttctttttc tccaattcca gtatcatttt tcaatgatag
     4621 caaacgccct tgttcatcaa catagcgaac tttcacattt tctgagatta gttctgccaa
     4681 ttctgaggtt tttttctttt tcttgatttc ttcggttatt ttccctttct cttcttcggg
     4741 aatatttagt tttggaatga ttttttcaac aacggttcgt gatggttcca cagtatcttg
     4801 gatgactgaa aagtcagcta gaattgggag attatactga acacggtgac tttgagtgtt
     4861 tactcctact ctttcattat tctctgaaaa tacttgtacg gtataagaaa caacatcttt
     4921 tcctaataga acatccccag tagagaaata gccgcctttt cctagtttgc tatctccaga
     4981 gtccacttct ttcctaatct tatcagatag ttttttacca gtcagtacat tcgttcgcac
     5041 aatccctttg tctaccccta caaagtggga gaactttttg aactcttcag aaccagatct
     5101 agcccaacca ttattaaggg catttgcttt tgtatttgta ttctctctca aaggtttggc
     5161 gattagaatt atattcatcg gcacttagag ttgctgctat atctgactct tgaataccaa
     5221 cttccttact accatttcta gcggcagtat atgtgtattt atctgtttct acttctagct
     5281 gattatcctt aacatgatag gtaattttct cctggtataa gagtcttcag tagtttatat
     5341 gctctgtcat catctttagc tttagaagca gctgagttta gccaagttgg ggtattagga
     5401 gtatctttat cagcctgttt caagacatta ttcaagtcat ttgctaacct attggttgtc
     5461 ctcactgtga aagttttctt ccagtctgaa tcacttttat tagacttaga tatgctcatg
     5521 aaatcctggt caacattcaa cacaatatcg ccttgttttt tagcatcttc cgttgcttct
     5581 gcttcaaatt gatcatggag tttttgttgc tctggtgtat agttttttta tttacacgat
     5641 ttaatgtact attttttatt ttagtattaa gaactccatt aatacgattt ttaaattctg
     5701 cttcaaaaaa tcccttaatt tgttggtgcg caagttcaac attcaaacct ttcttataca
     5761 agtcttcatt ctgatacaag tcttcattct gaacgatttc ggctacacgg tgaatggttt
     5821 gatcgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgatagg
     5881 ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca ttcaaaaata
     5941 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     6001 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     6061 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     6121 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     6181 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     6241 gaagttcgct caaaacgacg tccgctaatc ttttcataga ctttctcccc tctaggagcc
     6301 cctgcataag gacgatagag gtagcggtcg attcctgttt catcaatata tacgattggt
     6361 aaatctttta ggttatccaa aatatcaaga aactcagcga ctttctctga ctcttgttcc
     6421 ttaaagatcg tcatcttttt ttaaagtgac cttaatctgc tttaaagctg cccatactga
     6481 aggaacagca caatcaaaat gtgccgcaat ttcccgtaaa aaagcatctg gatgagcctc
     6541 tacaaaggct ttcaattctt ctaaagggat cttacgcttt ttgacgactc gctttttccg
     6601 ctctaagttc ctttgttcac gtctttcttt tcccacgtga agagagttct gacgccaaca
     6661 ccaaaaaact ttggccgcct cgacatgtct gtgccctcct ttgatggaat ctaatgctcc
     6721 ttgtttaaaa tcagtattat atgccatagc tttattagac catgcttatt ttaaactaag
     6781 caactatatc aatgttttgt tatgagaaga cttacttttt ttactgaaat tgaatttttc
     6841 ctagcctcaa catcatataa ccccaaaatt caatctgacc tggtttgaag agatgttcga
     6901 agcagataag gttttactaa aactaaaaaa aatgatataa tcaaactgat taaatgtttt
     6961 aaaaataaag gagaatttga atgtcttacc aagaaaatta ccagaaatgg gttgattttg
     7021 tggagcttcc tgactacctt cgtcaagatt tggaaaatat ggacgaaaaa actaaggaag
     7081 atgccttcta tacaaatctt gaatttggta ctgcaggtat gcgtggcttg gttggtgctg
     7141 gtacaaaccg tatcaacatc tacgttgttc gccaagctac tgaaggattg gctcgtttga
     7201 ttgagtcaaa aggtggaaac gagaaagaac gcggtgtagc aattgcctac gatagccgtc
     7261 acttctcacc tgagtttgcc tttgaatctg cggcagttct tgctaaacac ggcatcaaat
     7321 cttacgtatt tgaaagcctt cgtccgactc cagaactatc atttgcagtt cgtcatctca
     7381 actgtttcgc aggtatcatg gtcacagcca gccacaaccc tgctccattt aacggttaca
     7441 aggtttacgg tgaagacggt ggacaaatgc ctccacacga tgcggacgct ttgactactt
     7501 atatccgtgc aatcgaaaac ccatttgcag ttgaagttgc tgatgtggaa actgaaaaag
     7561 cttctggctt gattgaagtt atcggcgaag ctgttgacgt agaatacctt aaagaggtta
     7621 aggacgtaaa catcaaccca gccttgattg aagaatttgg taaagacatg aagattgtct
     7681 acacaccact tcatggtact ggtgaaatgt tggctcgtcg tgctcttgcc caagcaggat
     7741 ttgactctgt tcaagttgtt gaagcgcaag caactgctga cccagacttc tcaactgtaa
     7801 catctccaaa cccagaaagc caagcagcct ttgcccttgc tgaagaactt ggtcgtcaag
     7861 ttggtgcaga tgttcttgtc gcaactgacc cagacgctga ccgtgttggt gttgaagttc
     7921 ttcaaaaaga tggtagctac ctcaaccttt caggtaacca aatcggtgct atcatggcta
     7981 aatacatctt ggaagctcac aaaaacgctg gaactcttcc tgaaaatgcc gctctctgca
     8041 aatctatcgt ttcaactgac ttggtaacga agattgctga aagctacggc gcaactatgt
     8101 tcaacgtctt gacaggtttc aaatttatcg ctgaaaaaat ccaagaattc gaagaaaaac
     8161 acaaccacac ttacatgatg ggatttgaag aaagcttcgg ttacttgatt aaaccatttg
     8221 tacgtgataa agatgccatc caagccattc ttgtcgttgc tgaacttgct gcctactacc
     8281 gttctcgtgg tttgacactt gctgacggta tcgaagaaat ctataaagaa tatggctact
     8341 acgcagaaaa aacaatctct gttactcttt caggtgtcga tggtgctgaa caaatcaaag
     8401 cgattatggc taaattccgc aacaatgctc caaaagaatg gaacgcaaca gctatcactg
     8461 tcgtagaaga cttcaaggca caaactgcta ctgttgctga cggtactgtt acaaacttga
     8521 caactcctcc aagtgatgtg ttgaaataca cacttgctga cggttcatgg attgccgttc
     8581 gcccttcagg tacagaacca aaaatcaagt tctacattgc agttgtaggg gaaaccaacg
     8641 aagaatcaca agctaagatt gctaacatcg aagcagaaat caatgcattt gtaaaataaa
     8701 gacctataaa agatgagacc aaccaacctc atcttttttc gtatcaaatc taagcaattt
     8761 tagaaacttt atggcatact agacttatca atgaaagcga ggtaatctca tggaacaatt
     8821 tttagataac atcaaagacc ttgaagtcac tacagttgtg cgtgcgcaag aagctcttga
     8881 taaaaaagaa actgcaacct tctttatcgg tcgcaaaact tgcccttact gccgtaaatt
     8941 tgcaggtaca ttgtcaggtg tcgtagctga aaccaaagct cacatttact tcatcaatag
     9001 tgaagaacca agccaactca atgatttgca agcattccgc tcacgctatg gaatcccaac
     9061 tgtaccaggt tttgttcata ttacagatgg acaaatcaac gtccgttgcg actcttcaat
     9121 gtcagcacaa gaaatcaaag atttcgcagg attgtaaaaa atcagctaac aagtcttaaa
     9181 atcacaaaaa cgcataatgt aaggtgcaaa aaccttgata ttatgcgttt tattgttcct
     9241 aaaacagtta catcagcatt tcattacaga acattttaat tttggaagtc aaatcaattt
     9301 ttaacaatgt tttagaaatc acagtgggct attccatatt aaattatcca gattcaatcg
     9361 ctattatcgt tttcctagat tacaaaaaaa ctgcgttttg ctaatcaaaa cgcagtctca
     9421 gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc ttctccaaac
     9481 catttttggc tgatttcttg gaacttgccg tccttgtaaa gactagaaaa agcttcattt
     9541 atcttcttaa ccaaggttgt atcttcctta cgggctccaa ccgcaaaagc ttctgtttct
     9601 agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa ataatagttt
     9661 gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc aatcaaggct
     9721 tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt caaaatttct
     9781 ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa tgtctttcca
     9841 gtcatatcct ttgcagtcgt gataccagat gatttcttcg taaccaatac ctgctcattc
     9901 ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc ggaatagcca
     9961 ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc ccaatcaatc
    10021 ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa atcaatatca
    10081 aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt actatcaaat
    10141 ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact agtttcgcta
    10201 gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa tacaagcatc
    10261 cattttttca tccatttttc ctcctattta ggttctactt tcaataatac atctgcgata
    10321 ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag ttccctattt
    10381 tgcaagatta gcttctccac ttccaaacgt aattctggat ccagggcaga agttggttca
    10441 tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac ccgctgcttt
    10501 tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc taactgttcc
    10561 aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt cttcacaggc
    10621 gataaggtca aattgtccag aactgataaa tgagggaaca gttgaaaatc ttggaataca
    10681 aatcccagta gattgcgctt ctgcaattca tccagctcta aaggttgtcc attataaaag
    10741 atttgccctg aatcaatggt ttcaagacct gcaagcatac gtaaaagagt tgtcttacct
    10801 ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag actgaaatta
    10861 gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa catggcagcc
    10921 tcctatctat aataactata cttcttctca acttttttgg aaataattgt cacaatccca
    10981 atcaaaatca aataaatggc tcctgccaag aacataggaa ctagactagc atcgcggtta
    11041 gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac caaagaagta
    11101 tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt ggtcacttgg
    11161 ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt ggcggcctca
    11221 tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa gtaagctgca
    11281 tagttgagaa caaaggcaat aatagctgca ggaaggcggt ctaaacgaat cccaatactt
    11341 gggagcacat aatagataaa aatcagttgc aagagtaagg gggttcctcg cataacccag
    11401 atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc aaaggcaatc
    11461 aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac agtcgtgctc
    11521 gcaccattca gtaaactcgg taatatctca aataaataag acatactgca cctcctaaaa
    11581 gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg taaaaagatt
    11641 ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt tctatatgtt
    11701 taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg gaaccagttt
    11761 taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac gcaagagata
    11821 gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctatcaact cttgataatg
    11881 ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca tccacttggt
    11941 caaaagattt tctgactcct cactctgcaa gtctagaata tcctcataac gctcctgctc
    12001 cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt cttcttttgc
    12061 agtaaggaga taattttctg caccactagc atcatgcaat tcataagaaa attgtgaagc
    12121 agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag cgatacgcag
    12181 ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact catagccttc
    12241 aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt gatattcttc
    12301 ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt ctaactccag
    12361 ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat aggcaaagcc
    12421 aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat ctaactgggc
    12481 ataggcttga atagccgctt ggtaattttc caactcacta tccaactctg ccaaacccaa
    12541 tatcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac gtgccacatc
    12601 tgtcaaacct tccagctggt aaaggtctgc cttcagagcc aaagacgaga cataccagtc
    12661 actgtcagct tggatttcct caagataggt aaaggcttct tctatttgac catcctcgct
    12721 agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt tcaggtaaat
    12781 ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact catacagaag
    12841 atcacttgaa tcattttcta aagctttggc gaaataatgc tcagccttag ttaaatcttg
    12901 ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc tcaccctcta
    12961 attcatatag accactgact accttatacc attcgaaaat ggctgaaatg acaaccttag
    13021 cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata gatcctgaag
    13081 ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa ttgacttccc aaaatcaatg
    13141 gagagacaaa acggccttca atagtttgtt ctacgataaa gacaatcact actttcaaaa
    13201 gcatgactgg accagcaatc aaacccaata ctagagcagg aagcatggct agaaagctac
    13261 caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc gcatagcgta
    13321 gaccaataat cttgaagaag atgataaaca ttactgctac aataatagcc actgtcactt
    13381 gccctcgaac atagttggac aactgttgat tcacatctga taaaacttgt ccaacaggtt
    13441 ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc ccatcacgca
    13501 agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga gaagctccgc
    13561 taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc aaaactgtag
    13621 cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca tctggcaggt
    13681 gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca ggaacgtttc
    13741 ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag ccccaaatga
    13801 taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta accttatgct
    13861 tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac aaaatgactg
    13921 gcaacatcac aactgctaaa aagtctaaaa caggtgaaaa tagaaaacta atcttactta
    13981 aaataaaaag attcagtccc aataataagg ttactaaaaa taccgtaatt gccttgttat
    14041 ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc tccataaata
    14101 cttcctttct attgttctct cattatacca tttttgaaac aaaataactc ttttttaaag
    14161 tgcttacatc taattggata tctttaattt acttgttttt gtgatataat agtcttatct
    14221 ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac acgtcgtact
    14281 tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc aaatctagaa
    14341 ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca acatttatac
    14401 actgttggta gccaagacga taagggggga attgcagcct atcaaactga cgggactgtg
    14461 ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga tgaaaagcgt
    14521 gatttggttt acgcagccaa ctatcacaag ggacaagtcc ttgtttataa acgccaggaa
    14581 gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc acatgaaaat
    14641 caagcttccc cccatgttca ctatacagat ttaacacctg accactatct agtgacctgc
    14701 gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa attatctaag
    14761 ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt ccataaccac
    14821 tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt aatctacgat
    14881 ggcgttggcg aatttgaacg tatgcaggtc atttcaactt taccggaagc ttacgaaggc
    14941 tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta cgcttctaac
    15001 cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt agagttatta
    15061 gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc cgaccaaaaa
    15121 tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg taattgtgac
    15181 aatggtcgtc tagcagaact ctccaacgac ttccatgttc ccgaagcagt ctgcatccgt
    15241 tttgctcctt aaaacaccat aaaaaatgaa cttggtattt tcaagttcat tttttcttat
    15301 agtctatttc cgacattaat acggttaata gcacgttgca aagcaatctt agcacgacgt
    15361 tcttggtcaa tcaaatgttt gtcttgtgct tcttcaattg cacgttctgc acgaagtttg
    15421 gcacgttctg cacgactgat atcgatatca cgagcacgtt ctgcagagtc agcgacgatt
    15481 gtgatcatat cattggcaat ttcaataaca ccaccgttta ctgcaatcca gttcacgtga
    15541 tctttatcat cgatacgttt tacctttact tcatcaactg ctaaaaccgc aatcatattt
    15601 tcatgtcgtg gcaagatccc catctcacca tccagagttc gaaccgatac atagctggca
    15661 tggtgatcat agacgagacc atctggtgtc acgatctgga cagttaactg agccatagtt
    15721 cacctcttaa aatcccattt tttcagcttt tgcaatcaca tcttcgatag aacctacacc
    15781 acggaaggca tcttctggca agtggtcgta tttaccatca aggatttcct taaagccacg
    15841 tacagtttca gcaactggaa cataagaacc tggctgacca gtaaattgtt ccgcaacatt
    15901 gaagttttgt gacaagaaga actggatacg acgggcgcga gcaaccaagg tcttttcttc
    15961 atcagaaagt tcatccatac caaggatagc aatgatatct tgcaattcat ggtaacgttg
    16021 aaggacacgt tttacttcag cagcaactgc atagtgctct tctccaacga tttcaggtgc
    16081 caaggcacgt gagcttgaag caagtgggtc aacggctggg tagataccca attgtaccaa
    16141 cttacgttcc aagtttgttg ttgaatccaa gtgagcgaag gctgttgctg gcgctgggtc
    16201 agtatagtca tccgctggca catagatagc ctggatagag gttacagaac ccttcttggt
    16261 tgatgtgata cgttcttgca attgacccat ttccgtagca agtgttggtt ggtaaccaac
    16321 ggctgatggc atacgaccca aaagggcaga tacttctgaa ccagcctgag tgaaacggaa
    16381 gatattatcg ataaagagaa gcacgtcttg gccttccaca tcacggaagt attcagcgat
    16441 tgtcaaacca gtaagggcaa cacgcatacg tgctcctggt ggctcattca tctgaccaaa
    16501 gaccatggct gttttctcga taacgcctga ttctttcatt tcccagtaaa ggtcattccc
    16561 ctcacgagta cgttccccaa caccagcaaa tactgaaata ccaccgtgct cttgggcaat
    16621 gttgtgaatc aattcttgga ttaagacagt tttaccaact ccggcaccac cgaaaagtcc
    16681 aactttacca cctttaaggt aaggggcaag aaggtcaata accttgatcc ctgtttcaag
    16741 gatttcagaa gaggtagaca actcatcaaa agttggagct tttttatgaa ttggctgacg
    16801 ctctgcgtct tctgtaaaag gagcttccaa gtcaatggta tctcccaaaa cgttgaagac
    16861 acgtcccaaa gtttctttac ctactggtac agagattgga cgacctgtgt ccaatacttc
    16921 cattccacga gtcaacccat ctgttgattc catggcgata gtacgaacca taccatctcc
    16981 taactccaag gctacttcaa ggacgatttt tgtttttctt tcgtcatttt tgtagacgac
    17041 aagtgcattg ttaatctcag gaagtttttc ccctgctgca aacaaaacgt ctacaacggg
    17101 accgataacc tgagcaattt tacctgaact catctccttc tcctattcta tataagatac
    17161 tgtcggttcc tagctcaact aggtcctaag ttcattttca tacgagctgg actagagcct
    17221 attctaaggc actagcacct gctacgattt ctgtaatttc ttgtgtaatc gccgcctgtc
    17281 tggcacggtt atactgaatt gtcaaatcat tgatgacttt cttagcatta tctgtcgctg
    17341 tttgcatggc tgtcataccc gcagcattct cagctgtctt ggcatctata atggcaccgt
    17401 aaatcatact ttctgcaaac tgaggcaaca actgctccag aatttcttct cggctggttt
    17461 ccaattcaaa agtcaagctg tactcttcat ccgcttcatt tggatccaag tcaacaatcg
    17521 gaagcatttg ttccacacgc atttgactgg ttagcgtatt gacatggtgg ttgtagcaaa
    17581 cataaagctc atcaaagagt tcattttggt acatttcaac agtttttgaa ataatcttac
    17641 gaacttgatc aaagctaggt tggtctgcca agccacgtaa ttcataaagt ggttgaatac
    17701 cgcgagcctt aaagaaatca gctcccatcc caccgataca gatcatttca aaacctttac
    17761 cgtctgggtg gtattcttct ttcaactcca taacagcttt caaaatagag gaattataac
    17821 ctccaaccaa accgcggtct gaagtgataa cgatatagcc tgtcttcttc acagaacggc
    17881 taatcaacat cggattagtt gaagcaccag ctccattacc atgaaggata tctgtcaaaa
    17941 gtttacgcac tttctgagcg taaacttgga agttgcgagc agcttcttca gagcgaccta
    18001 gcttagcagc cgataccatt tgcatggcat tagtgatttg actcgtattt tttgttgagg
    18061 cgatttttgt tttaatatca tttagagata ctgccatctg acacctctat tcttattgga
    18121 agctagattg attgagaaac tctgtaatcg cagcatccaa gactgcttct tctggcaagt
    18181 cttttgtatc acgaatggtt tccaaaatct ctggatgttg agcatcaaag aaggcatgga
    18241 actcttcctc gaaacgaaca atatcatcta ctggaacagt atccaagaaa ccatgtgtca
    18301 aagcataaag aatggttact tgtttctcaa caggtaatgg tttgtgaaca ggttgtttca
    18361 agacctcaac ggtacgacgt ccacggttca acttagcctg tgttgctgcg tccaagtcag
    18421 aaccaaactt agtaaaggct tccaactcac ggtatgaagc aaggtcgata cgaagtgtac
    18481 cagcaacctt cttcatggct ttgatttgtg cagaaccacc tacacgagat acagatgaac
    18541 ccgcatcgat ggctggacga atacctgcat tgaagaggcc atcgccaagg aagatttgtc
    18601 catcagtgat agaaatcacg ttggttgcga tataggctga gatatctcct gcttgtgtct
    18661 cgataaatgg tagggctgta attgatccac caccaagttc atcagaaact ttagctgagc
    18721 gctcaagcaa acggctgtgg agatagaaaa catcccctgg gaaggcttca cgacctggag
    18781 gacgacgaag caagagcgac agttcacgat aagctaccgc ttgttttgat agatcatcgt
    18841 atacaatcaa aacatgctta ccttgataca taaattcttc cgccatagcg accccagtat
    18901 aaggagctag gaagagcaat ggagatggtt gtgaagcaga ggctgtcaca acgattgtgt
    18961 agtccaaggc accgtactga cgaagtgttt ctacttgcgt acgaactgtt gattcttttt
    19021 gtccaatcgc gacgtagata cagatcatat cttgatcttt ttggttcaag attgtatcaa
    19081 tcgcaatggt tgttttccct gtctgacggt caccgataat caactcacgt tgaccacgac
    19141 caatcggtac aagggcgtca atagctttca aaccagtttg caatggttct gaaacagact
    19201 tacgttgcat aacaccagga gctggtgctt ctactggacg agttttatca gtgtggattt
    19261 ctccaagacc gtcaactgga cgaccaagcg gatccacaac acgaccaatc agactttcac
    19321 ctacagggac ttccatgatt ttccctgtac ggcggattgt atcgccttca cggatatctg
    19381 taaagtcacc taggatgata ataccaacgt ctgttgactc caagttttga gccataccat
    19441 aagagccgtt ttcaaaattc aacaactctc cactcatgac attttcaagg ccgtgagcac
    19501 gcgcgatacc gtccccgata taggttacaa cacctgtttc agtcacatca aaattgggtt
    19561 tgaaattttc aatttgttgc ttaattaaag cgctgatttc ttgtgcgtta attgccaaaa
    19621 gaacaccact ttctatttca aattttcttt aacaacttta agttgttgtt taatactcac
    19681 atcaattgtc ttgtgattgg caaaaatgac aaaaccacca atgagacttt catcgatttg
    19741 ttcttttaca ctccttactt tcagagacat ttttttctca atcaaaggga gcaaacgagt
    19801 cttctgttca tcagttagag gatgagcaga cgtaatcgtc acttcaaatc gatttgtttc
    19861 tttttcaagt cggttcaagc aatctacaag cacatcataa aaaagatttg ctctgtgatt
    19921 gtaggccaga acctggataa agttttgtaa taaaggcgac acagaatctt ggaaaaaagc
    19981 aattgttttt tccttatccg actcgtctac tgccacttgt tttaaaaaag aaggcagacc
    20041 tgttttttca acaacttgct tgatttgagt caagtctgaa aagatacggt cttcttctcc
    20101 tttttcaagt accaattgga caaaaggcat gctgtatttt tcaattacct ttactgtttt
    20161 cttgtccatt aagcttctcc tagctgatcg atatactgat caatgagtgc tttatgggca
    20221 tgactgtcaa ggttttgtga gatgatttta ccagctaagc tgatggtcag atctgcgacc
    20281 tcacccttaa cactctgtaa agcttctact ttattttgag caatttcttg attggctttt
    20341 tcttttaagt gtcctgcttc tagtttagca tctgctaaga tattagcctt actttgctca
    20401 gctgtttcct ttgcattttc aatgattgtc ttagcttctt tacggctacc agccaattca
    20461 tcttcgcgtt tttgagccaa tacttctgct ttttgacggg cttcttcagc tctgtcaata
    20521 tctgaagcaa ttttttcagc tctttcttcg aaaatgcctg taatattaga ccatgcaaat
    20581 tttttaatca agactagcaa aagaataaaa gagccagtga ttaaaataaa attaccaatt
    20641 aattcaccta ctgttacgtg catcagttac tcctttctat tcctcttcaa tattaatctt
    20701 attccctaaa tacacagatg tcaaaagagt aaaaacataa gcttggatgc aggaaataaa
    20761 gacagaaaat gcagtccaag ccaaattagc tccaaaggct actggatacc aataaatagc
    20821 ttggtgggaa agaagaagta acaaactcgt catgacctct cctgcaaaga tattcccaaa
    20881 aatccgcaaa gccaaagata agaagtttgt aaattcttcc aagatattca tcggtatgac
    20941 aaaaacagga ctcataaaag attttatact ttttttaaat ccacgacgac gaacgctttc
    21001 tatatgtgtc aacaaaatga caagaaaaga taaggttaag tcatactgta aattagcggt
    21061 tggcgaactc caccagttag tcccatcgat cgtttgaagc tttgtcatta agccaaggtt
    21121 attggcaatc accatgaaaa ggaataaaca aaggaaaaag agtgaataat ctttcatgta
    21181 gcgcgaacca atattaggtt ctgtaaatcc aataacaaag tcatagacat actcaagtac
    21241 attttgcttt cctttgggtt tcaaggtcat attgcggctt gcccaataaa taaagacaaa
    21301 aataactccc acaatcaaca aagtcatggc taacatagtc agattgaaga taacaggacc
    21361 aatagagatg attggattaa tactttcttc catctaaaat cctccctttt cttctcgttc
    21421 catgtattta tttgataata aatgagaaga caagagttac aaagaaagtt ccttcaataa
    21481 aggcaacacc taaaaacatc aaactacgaa actcagaaag catatctggt tggcgtgcta
    21541 ctgatttaaa cagtccattc atcaataaac cttcaccgac agatacgccc atacaggcaa
    21601 tacataagcc taaaaatgtt aaattcataa caaattctcc tttaaaataa aatttactta
    21661 cttagtgtag tcctaaaatt taaatttgtc aacttttaac cgctattgga agcgtttaat
    21721 aaaaattatt tccattttgc taaatttcaa gttcaagtta gccatccaaa agtcttctct
    21781 gggtgacttg tagtccaagc attttttagg atagttgtta atccactttt cgatgaatgc
    21841 gacttctttg ggagtcattt tcttggttcc cttaggtaac catctacgaa tgagcctgtt
    21901 gtgattctca ttagttcccc tttcccaaga ggcataggga tgtgcataat aaatgtgctc
    21961 ctcagaaaat acattagaca agcgattgaa ttccgttcca ttatctgccg tgatggaaag
    22021 aatcttgtgt tgttttaaga tgagttttag agcctgattg accacctcag cacttttatt
    22081 tggaatcaat cggatgatct gatgtctact ttttcgatcc gtcaagacaa gcaagcagta
    22141 gtttttcgct ctcgtaagta gaactgtatc aatctcataa tgcccattct ccaagcgaaa
    22201 attgatagct tcaagccgct gttcgatgga ttgaccagca ggtttaaagt tggtgctagc
    22261 ctgtttctta agcgcttttc cttttctagg gtaaggcaaa tcctgcttgc ttaaccccaa
    22321 ttttccatga tgaatccaat agtaaatggt tgaaattccc acgttaaccc ctttagccat
    22381 aaccatcatt tcaggcgaaa atttttggtt atgatagtgg agaatctttt cctttagttc
    22441 cttggtcaag cttgatttct tgaccgagcg cttgcgattg ttttcataag actgttgagc
    22501 atagtcggca gaataaacct ctttgaagcg cccttttcca agacattgtc ggactgtcct
    22561 acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg caatttctct
    22621 atttgatttc cctttgttcg ccttttgtag tataatggtt ttgcatctct gtgcctttct
    22681 tgtgtttgtg gttgaacaac aagtataaca caaaggtgtt ttcttatgcc tacaagagct
    22741 ttcattattt ccattttctt ttggatttca ctctattctg aaaaacttgt gtatatttac
    22801 tgaagctagc aagtcttacc tgtaaattta atgaaagcaa cacaaaatcc gagaggggaa
    22861 tctcggatta atgtcaattt aaaaagatag agagttttta gtttaaataa attgttttaa
    22921 aatatcaaca acatcacttc ttttcttaac ctgataagcc ttgattccta attttggggc
    22981 tacgattata ttgtcctcaa tatcgtctag aaagacacaa tttctaggtt ataactggta
    23041 tttatcgata gttactcata tatatcagtc cacctccata ctcaccttac agattctttt
    23101 gataataata tctttgccta atgtagagat agtcttgcaa agaaaaaact tccttgtagc
    23161 catgtttctg ataaaagtcc ggtgcctgga actggtaagt attgacaaag gcaaaacaac
    23221 aatttcgatt cttagcttca ctttctgcct gttgcaatag ttttgaaccg attccttgcc
    23281 ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca aaagtctctg
    23341 ctatcaaacc cgcaaggaga ttgccctttt catcttcgac ataaagatta agtggctcac
    23401 tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct atttcttgcg
    23461 atttatgtga ttccttattt tccaatctaa agtataatga aatgaaataa aacatgcgca
    23521 aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa gtgtactatt
    23581 ttaacttcaa tgcactatac atctaatact caataaaaat caaagagcaa actaggaaac
    23641 taggcgcagg ctgctcaaaa caccgttttg aggttgtaga tgaaactgac gaagtcagct
    23701 caaaacatgg ttttgaggtt gtagatgaaa ctgacaaagt cagtaaccat atatacggca
    23761 aggcgacgct gacgtggttt gaagagattt tcgaagaata gcaaaatgga aaaaggagtg
    23821 agtgaagcac atcgcctccc cactcctttt tctgttttta ggctgttttt tcaaccttca
    23881 agatttttac atcatagcta ccaacaggcg tttcaatggt tgctgtgtca cctgttttct
    23941 tgccaatcaa ggcctgccca attgggcttt catttgaaac cttacctgca aaggcatccg
    24001 caccagctga acctacgata atataaactt cttcttcgtc ctcaccaatt tcttggatgg
    24061 tgactgtttt accaatcgct acttcgtcct gggcaactgc gtcgctattg acgatttcag
    24121 catagcggat ttttgtttct aagctagaga tttgtccttc gacaaaggct tgttcatcct
    24181 tagctgcttc gtactcactg ttttctgaaa gatcaccgta tgaacgggca atcttaatgc
    24241 gttctaccac ttctggtcga cgaaccaatt tcaattcttc taattctttt tcaagtttct
    24301 ccttttcctc aagggtcata ggatatgttt tttctgccat ttttctcaac tttcttctga
    24361 taatattttc taaagaaaat tatgtgaagt atcacataat tttagtttgt ttagtttaat
    24421 ttgctgttga catgttcagc gacattgcgg tcgtggtctt cttgattgtt agcatagtag
    24481 accttgcctt ctgtgacatc tgctacaaag tagaggttat cgctcttagt ttgattgatg
    24541 cttgactcaa tcgcatccag acttggacta tcgactggac caggcatgag acctacattt
    24601 ttataaacat tataaggtga atcaatgtcg gtatcaatcg caacatcctc agctagactg
    24661 atattttgcc ccagttttcc ttgggcatac aagattgcaa tattactttg aagtggcata
    24721 tcacgattta aacgattgta gaatacacct gcaatgagct tacgatcttc tgtcttggca
    24781 ccttcttttt cgaccaagga agcaatggtc aacaactcat tgatagtcaa gtttttagat
    24841 ttgatagtac tatagtaagg agataggttc ttatccatag cagctaacat ctcatcaatc
    24901 aagctctcaa tagttgtgct ttccttgata gagtatgtag ctgggaaaag gtatccttcc
    24961 aaacgataac gcgcaccgct gtcttttaca ggcaaacttt ccagtaaagt aggatatttc
    25021 gctactgctt gactgataaa cgtctcatct tgaactttag ccaagaaagc ctccgctgtc
    25081 aaagactctt tgaagtcacc ttgcaattga cccacagttt gagcaatctg atccaaggta
    25141 taaccttctg gaattgtcaa agtcgcaagt acaggttctt gcggttcatc tgttccacct
    25201 ttttgcaact ctttgagtaa gtcttctgta ctcatactct tttgcaaatt gtagtaacct
    25261 gctttcaagt cggtataatt tttatacttg gcataaaaac taaaaatcag accatgcttt
    25321 accaaaccag ctttttcaag cgtcgtaccg atttcttgaa cgtttgaacc ttctggaatt
    25381 ccaaccgtca catatttctt agaattagca tcgataggta acaaagaatc tagcacgtac
    25441 tggtaaccaa agtaaccacc tgctgagagc aaggcaagga atacaaggaa tgaaatcaca
    25501 aaagctttca catgtgactt cttctctttc ttaggtttaa cgggttttgc tccttctcta
    25561 cggctacgac gtgatgtatc gctgatgata tccactgttt ctgtagcagg cgtaggtgtt
    25621 tctggacctg cttgttctgc ttttttctct gccgtcgtat aggaaacagc tacccttgtt
    25681 ggggtttcat tgtattctct ttcaagtttc ttaggtctaa caggacctgg acctggtctt
    25741 gatccacttt cttccgctgg agaagaaggt acatcttgac ttggatgact tggaacacca
    25801 ggagtttctc tttgaatctc atctgctgga gaagctggta caccttgact tgggtgagta
    25861 ggcacggtag gagcttttct cataatctcc tctaccgttg acaaggaatc agccatgagt
    25921 tcttcagctg aaggttcatt tgcaggagtg cgaactactg cctcatcttc tttcagaact
    25981 tcatcatagc cttttacttt ttctaaatct ctcagaatct gctctttaaa gcttaatttc
    26041 tcttcttctc ttgacttttc actcaaaagt ttttcctcct tgttgataat ccataatatt
    26101 atatcttaaa agtccaaaaa aagcaatcta tgatactttt cctaacttat tttttcattt
    26161 cccagaccat atcataccat gtttcccctg caaaggttga ctgggactgg ccttcgttga
    26221 caaatccatg tttttcatag taggcgatga gatagtcatg acaggttaga ttaatgccat
    26281 ctctctcatc ttcaagagca acttctttca aggctgtcag caatttctga cccagtccga
    26341 gtccctgtgc ctccttggca atagacagac aggtcacaga gatataacca ccaggctcat
    26401 gactatagtc ttttatttct tctgtaaaag actggtcttg cagatggcgg tgcaggccaa
    26461 ctggtccttc gatataaccc atgattcttc cttctttttc agcaaccaga aaagaggtct
    26521 gaatttctct caaatgtgct tcaaagacag aaggaggaat ggcttcttcg accgaaaaat
    26581 tttcaaattc aagttcaaca atccgatcca aatcttctaa tcttgcttgt ctgattttca
    26641 ttgttcctcc agataaaagg gattaaacca aatcatacta tagccctggc tagttgcata
    26701 gagcaaagtt tcttcttcat caacaaaacc gttcatttca aaataggaaa gcagctcatc
    26761 aggactctcc aaacgaatcc ctttgcaatc cagctcaact gccacctctt tcaaggctgc
    26821 aagaagaagt gttcccaggc cctgtctctg atggtcagac tcgatgacta aagaatgtac
    26881 ttttagacat tgcggattgt ctgactgggg acttgataaa atatagccta aaagttgatt
    26941 ttcatcccta gctagaagaa aggtatccgc acacttacgg atactttctt ctaaaatatg
    27001 ggaaagttgc tgcttttcag ctggaaaaga cgaggtctga agtgccccta tctcaggcaa
    27061 atcagacttg cttgcctgaa tgatcttaat tggaatttcc atgggaacat cctattgaac
    27121 attgcttgtc aagttagata agagacgctc aaatgagtat tcataggttt ggatgtctcc
    27181 tgctcccata aagacgtaaa cagcattgtc atggtctagg agtggagaaa cattttcaac
    27241 agtaatcact tggtgttttt tgttgatttt atttgctagg tcttctacct taacgtcacc
    27301 atgatctact tcacgagccg agccataaat ttgcgctaga taaacagcat ctgcttggtt
    27361 taaagcatgg gcaaagtcgt ccaacagggc aatggttctt gtaaaggtat gcggttgaaa
    27421 gactgctaca atttccttgc ttgggtattt ctgacgagcc gcatccaagg tcgcaataat
    27481 ttctgttgga tgatgggcaa agtcatcaat aatcactgta tcattgacaa ttttctcagt
    27541 gaaacgacgt ttaacaccgg caaatgtttt caagtgctca cgcaccaagt tcaaatcaaa
    27601 tcctgctgtg taaagaagac caataacggc tgtcgcattc atgatattgt gacgaccaaa
    27661 ggttggaatg tggaattgcc ccaagttttg tccacggaaa tgaacggtga aggttgaacc
    27721 agttgttgaa cgaagaagat cactagctac aaagtcattg ccttcagctt caaaaccata
    27781 ataataaatt ggtgcatcag acgtaatctt acgcaattca gcatcttcac catagacaaa
    27841 aagacccttg gtaatttgtt tggcatagtc gttaaaggca ttgaaaacat cctcgagact
    27901 tgtgaaataa tctggatggt caaagtcaat gttggtgata atagagtatt ctgggtggta
    27961 aggcatgaag tgacgctcat attcgtcaga ttcaaagaca aaatatttgg cattagccga
    28021 accacgacct gtcccatctc caatcaagaa gctggtatct gtaatgtgag acaagacatg
    28081 agacaacata cctgtcgttg aagtttttcc atgtgctcct gctactccca tgctaacaaa
    28141 gtcacgcata aagctaccta gaaactcatg gtaacgtttg tagctgatac cattttggtc
    28201 cgcataggca atttcgacgt tgttatctgg acgaaaggca tttccagcga taatttccat
    28261 atcaccgtct agattctttt catcaaaagg aagaatggta attcctgcct gctcaagacc
    28321 gcgttgggta aagtagtact tttcaacatc tgatccctga accttgtgcc ccatctggtg
    28381 caacatcaag gccaaggcac tcatccctga tcccttaatt ccgataaaat gatatgtctt
    28441 tgacatgttt tctcccctat tctgtcattc tggtcagatt caactcttgg gcaacccgac
    28501 gttcttgttc tgtttgttta ctttttttat tgtagatttg gctcttcttt agaaaatcat
    28561 aattgttttt ctttggagca ggtgctgaca cttcttcatt cttggtaggg atagaatgaa
    28621 cttcttccgc caagatataa tgagactggg tcaatttttg gctatatttg acaaattcac
    28681 caggattttc cttttggaaa ggagttgtcg gttgattgcc ctgtctaact agactgggct
    28741 gagaatgacg tctcgcaagg ctgaaatcct gagttaggta gttagcagag cgtttctttt
    28801 tcaagtccgc acgcgcttct tcacgcgcca cctccgcata gctctttcct tcttttttaa
    28861 cccctaaagg agccttttta ggtttttcga cttgcttttc aatcggtttt actggttttt
    28921 cttcagtaat aggagcccat tctaaataat ttttatctcg atactcaccc ttgatattac
    28981 tgatcagatc agactcatca tagagattca tgactggcat ttcagtcaac atgacctcgt
    29041 catctgacac caatggaaat cgttcttgtt tcattttcta tttcctttca acacttcatt
    29101 atagcgtatt gtcttgattt ttcaagtgct ggcttcagaa attcccaaaa tttctctaat
    29161 ttctgctagg gtcagactac cacgtgactc tgtgccatcc aatacttgtg acaccagatg
    29221 tttcttttgt tcttggagtt cctgaatttt ttcttcaatg gttcccttgg tcaccaagcg
    29281 atagacctca accgtttctt cctgacccat ccgatgggca cggccaatgg cttgcgcttc
    29341 caccgcagga ttccaccaaa ggtcaaccaa gatcactgta tctgcacctg tcaggttcag
    29401 accgacccca ccagccttga gggaaatcag aaaggcatct ctttctcctt ggttaaaggc
    29461 cttggtcatg tcttgtcttt ccttggctgg ggttgaaccc gtaattttaa aggatgtcag
    29521 gcccaagtct ggcagttctt gttcaatttt ttccaacatt cccttgaact gagagaaaat
    29581 caagacacgg tgtccgccgt ctgccacctg taccagtagg tctcggagac tatctagttt
    29641 gccgctggct ccctgataat cttccataaa cagggcagga gtgtcacata tttgacgcaa
    29701 gcgcatcaaa ccagataaaa tttccacacg acttcgctga aattcctgtt ctgacacttg
    29761 agccagatgg tctcgcatct gttgtaactg ggcaaggtaa atagcctttt gctggtcttc
    29821 cagttcattt ttataaacca cctcaatcaa gtctggcaat tcagtcagaa cttcttcttt
    29881 cttgcgtcgc atcacgaaag gcttgataaa ctgagccact cgctctgctg gcaatttcat
    29941 aaattctttc ttgcttggca aaagtccggg catgacgatt tggaaaatag accacaactc
    30001 acccagatgg ttttcaatcg gagttcctga caaggcaaag accgacggca ccacaaattg
    30061 tctcaaggtc tgggcaatct tggtctgggc atttttcatg acctgagcct catctaagaa
    30121 aaggaagtca aaggccatcc cctgataaaa ctcactgtcc tgacggaagg tggcatagct
    30181 agtcacatag atttgatggc tctcggcaag aatctcctca cgacttgctt tcaaaccatg
    30241 aacaacagcc acatccaact gtggggcaaa tttctgaaac tcatctgccc agttgtaaat
    30301 caaacctgac ggagcgagaa tcaaaacccg actttctttt gtcacttgac tagtcaaaaa
    30361 agcaatggtc tgaagggttt tcccaagtcc catatcatca gccaaaatcc caccaaaacc
    30421 ataatgatgg agcatctgca accagccaat tcccttttcc tgataatctc gcaagtcagc
    30481 ctgaacctga gttgcctgtc gaggaaagtc ctctggatgt gtcaaatcct gggccagatt
    30541 ctggaattct tgtgaaaaag aaacacggtc tcgcccttca aagagatgag ctaaactgta
    30601 ggccaaggat ttccgagcct gcaaggtccc atcttttaat tcaaattgcc ccagttcctg
    30661 tagattttgg cgaattttct tggtttcttc atcgaaaaag taaacttgat tagacgaatc
    30721 aatataaaaa tcctgattgg caaccaagac ctgcatggct tggtcgattt cctcctgggc
    30781 aatatcttga aaatcaaact ggatttccaa gagacctccc ttggaggcaa tctgcacctg
    30841 aggactcgct aggctataaa gctcttctag tttatctgat aggtcaacat gcccgagttt
    30901 ttcaaagact ggaatgatat catgaaaaaa atgatagaca gactccgctt ttaaggcctg
    30961 acgccaagat tgaaaatcgg cttcaaagcc cgcagccaaa cagacttgga aaattctttc
    31021 ttctaagtcc gcgtcacttg aaaatggtaa ttcttctagc tcttgtcggc tagatacctg
    31081 tctatttcca taatcaaact gaatttctaa acgaatccga ttatcttctt ccctgtcaaa
    31141 gtaaaaagag ggcgcaaaag ttttgatttg tagacgttct ggagctgaaa cggtgcccat
    31201 ctggataaaa agagtcagac aggaggccaa tttgtctcga tcactgctat caaattgcag
    31261 gtatttcttt ccttgttgac ccacaggtaa cgctttaatt tccttgagaa gacgcatctg
    31321 ctggtctgtt aaaaaataaa cctgaccttt atggaaaagt actgctccct gataaaagac
    31381 attgacccta ggactctcac tgatttccat ttcaaaatag tccgagtatt ctgttactgt
    31441 aaaggcaaat agattggcat cagcatgcat atcctgaaaa agcagggttt ggtagctatc
    31501 cacttgatgg tcaaattgaa aatggggcaa ggccatcagt aaattcacac cctgctcaaa
    31561 aaaggtcaga gggaaaaaga ggtgccgacc ttggttttgg aaaaagaggt ctgaaaccag
    31621 cccttcctcc gttagtccgt gcaagaaagt caaaagttct tggctggcat catcaaaggc
    31681 ttcccaagaa agagactcct cataaatctt gccaatcata tacgactttc tctgctcgac
    31741 aatccttaaa aaaagtggaa tatcccgaat gacatagtat ttttggctat tgatttggcc
    31801 gattctcaga gtccacaaga tatgattggt tcctgcttcc acctgaccca cagctgataa
    31861 ctcataggcg cattctgatt ttggagataa aattcgatcc aaaaacttgc cacccaaggt
    31921 taccttggtt tcaacagcct ctttttcttc atgaccttct tccagactcc acaagatttc
    31981 ctgaccacgc tcatcatttt tcagaaaatg ctctagcgct gccaaatgca cacagtaacc
    32041 cctcttttga aaaaaatcac aggcacaaaa aaccaaatca tcctctaaac tatagcgcag
    32101 ttcttcttct gcaacgcgag cgtagagccg attgttcttt tccttgatga tatcaacctt
    32161 accagtttca taaagggcaa caccttcgat acgaattttc cccggaatca atttagccat
    32221 attttcacct ttaccttatc tttttattat accatatttt cgcctatgaa aatagccttc
    32281 taggaagact tttctcctag aaggctggat ttttaacgtt tggcaaaagt agccacaatc
    32341 cgctgacaga cttcttgcaa cagagatttg ggcatagcta tattgatgcg ggcatggaga
    32401 cttccttcct ctccaaaatc caaaccacgg ttgaggataa ccttggcttc atttctcaac
    32461 aactcttgca atgtttcatc agtcaggtca taagctgaaa agtcaagcca aatcaagtag
    32521 gtaccttgcg gtttcatgac cttgatttta gtctcttttc caaatagatc caccacataa
    32581 ttgatgtggt cttcaaagac ttgcttgagt tcctctagcc aatctttacc gtatcgatag
    32641 gcagcttctg tcgccaaata acccaagcct gaaatttcat gctgattatt ggccaacagg
    32701 cgtttctgga aagctagtct caacttagga ttttcaatga ctgcatagga attttttgtt
    32761 ccagcaatat taaatgtttt agtggcactg ctcaagacga tagcaaaatt tttgaaggca
    32821 ggattgatgg tattgaaaga ctggtgtttg tgaccaaaga gggtcaaatc ttggtgaatc
    32881 tcatccgaaa ctaacaaaac accgtgtttt tggcagagtt ggccaatctt ctccaacact
    32941 tctttttccc aaacacgtcc accaggattg tgagggttgc aaagaatata gagtttaacc
    33001 tcctcttcca ccaaatcctt ttcaagttgg tcaaagtcaa tctcaaacag accatccttt
    33061 tccactaagg aattagtaat caatctacga ttattcaact tgacactgcg agcaaagggt
    33121 gggtagacag gcgtgttaat taaaaccgcc tcgccttctt ttgtaaaggt ttgaatagct
    33181 gttgagatgg ctggtaccac accctcgata aagacaagag cctctttgtc aaagttgtaa
    33241 ccgtattgtg tagcttccca cttttgaact tccttaatta agtcttcact ggcataggta
    33301 taaccataaa ccagttggtc tgcgtaaatt tgcacggctt ggcggatttc aggcaagacc
    33361 acaaagtcca tatccgctat ccaagctggt agaacttcac tatccgtttc tgtttctttc
    33421 catttatagg tatggtgccc taaacggttg ggcaggcttg taaaatcata ttttcccatc
    33481 tttgtcttat ccttctaagg cttggcgcaa atctgcaatc aaatctctag catcctcaat
    33541 cccaatagac aaacgcaaga ggtcatctgt caaaccataa gaatggcgta cttctgctgg
    33601 aatatcagca tgagtttgag tcgttggata agtaataaga ctttccactc cgcccaaact
    33661 ttccgcaaaa gagaagacct tgagactgtt caaaatatga ggaatgcgtg tttcatcggc
    33721 tactttaaag gaaatcatgc ctccacgacc agtgtagaga acttccttaa ctgctggaga
    33781 atccttcaaa aaggcaacca cttcttgggc gttagctgtt gagcgctcca tacgaagaga
    33841 caaggtcttg agaccacgaa gcaactggta gctgtcaaat ggagacaaga ctgcccctgt
    33901 tgtattgaga ttgtaaaaaa gcttctcgta tagttctaaa ctattggtca caaccactcc
    33961 agccaagaca tcattgtggc ctgctagata cttggttgct gaatggagaa cgatatctgc
    34021 tccatcttca atcggacgtt ggtagatagg gctatagaag gtattgtcca ccaccacttt
    34081 ggcaccctta gcatgagcca attttgctag tttttcgata tcaaattcca acatcaaggg
    34141 attggttggg gtttcgatat agagaacatc cacatccttt tctaactcgg caatcaactc
    34201 ttcttctgta ttggcatagg taaaatggaa acgaccttcc tgctccactt ggttaaacca
    34261 gcgaaaagaa ccgccgtaaa gatcacgcac tgccaagacc ttacttccta ctggaaagcc
    34321 gctaaaggcc agtacaatag ctgacatccc tgagctagtc gctaaggcat agtctgctga
    34381 ctcaatagtc gccaagactt cctcagcctt actacgagtt ggatttttag tgcgcgtata
    34441 gtcaaaccca gtagatcgac caaactctgg atgctgatag gtcgttgaaa aatgaagcgg
    34501 tgtcaccaat gcacctgtcg cttcatctga cttaataccc gcctgggcca aaattgtgtt
    34561 aatgtgtaat tccttgctca tacaatacct ccaaatctat agtaactatt gtaccactta
    34621 ttttcatcaa ctcattttct tcttttcaag agctagttat agtttcaaac tatataaaaa
    34681 gggagttttt cctgctccct ttaatagact ataaaatggt gaatctcaaa agacacttca
    34741 ctctatcatt tgctcctgca caaaacaagc ataacgctca tgattttcca gtagttcctt
    34801 atgagttcct gagccagtga ttttcccctc ctctaagaag aaaatacaat ccacatcttt
    34861 taccgttgac aaactatgcg ctataatcac aaccgtcttc tcctttaata cagaatagag
    34921 gctactgata atcgcatact cagaatccgc atcaagatta gcagtagctt catcaaatat
    34981 aagaatttca gcatctttta agtaggttct agctatttga agtctttggc tttgcccccc
    35041 tgacaagaat cgtccgcgtt caccaacttc agtatctagt ccctctttca tggagcgaat
    35101 ctcatcacct agtgatacta agtctagcac tttcatcaat tcatcatcag ttactaagcg
    35161 attcaaaccg agacaaagat tgtcacgaat actgccagat aagactgcat tattttgtga
    35221 aacccaagcg attttacttc tccattcttt taagttaaaa tcatatatac ttgattgctc
    35281 cattagaata tctcctgaaa gcggtttata aaaccgctct aacaaacgca caatcgttga
    35341 ttttcctgat ccagatggtc caacaaaagc aatttttgcc ccttgaaaat tgaacaagta
    35401 atatccttta agacaggtcg attttcatca taaccaaaat agacatggtt aaaattcaac
    35461 ccttgtcctg ataccgattt tcctccctca aatttttctt taggaactgc aagcaagtta
    35521 tccagtgcaa ctgaagatcc cttgctccta gaataaacag ttacaaaatt agctatatta
    35581 ctaataggat taagtaattg aaagaggtaa atcaaaaacg aaaccaaggt tcccacagat
    35641 atatatcctg cgctgacccg ataaccccca taggttagca tcacagctat agtcgcaaag
    35701 ataaataaga gagcaaacgg ggtctcaaaa gaagtaaccc tatctgattt cagtgaattg
    35761 ttttgtaccc tttcaataca attatccaaa acatcctgta cacttttctc tgcttggtta
    35821 gtcttaatta attcatgttc ttgaatcttt tcagtcaatt gccctgttaa atttcctcct
    35881 gtaaacgacg actatacttt tcactgatat tggaaagggg caagataata aacatcatac
    35941 aaggaagagt gatgaataaa agtagagaaa gattccaatc aagactaaat aagactacaa
    36001 tggaaccaag taccataact aaactcagaa taatatttgg gaaagtcgta attaaaaact
    36061 cacgaatgac actcatgtca ttgacaatgg cagaagtcaa ctccccactt tggctcttat
    36121 caaagaagga tttctctgca taaatcaacc cctctatcac ttttttcctg atttttgcta
    36181 tctttttttc acccgattga ctaaacagat agtaaccaat agaagaaaac aaggcttgac
    36241 caatacgatt gaaatacttt ggaacctata ttttcaatag aactcccatc tattaaaccc
    36301 tttaagataa ggggaagcaa caaagcaagt agactagaca gaacaagtaa gaaactcccc
    36361 ataatcacct tagtatctac tcttaataat tttaatttca taaatactcc ttataatatt
    36421 tcaacggata aagtcgggaa taactcaatt tgaggataaa atctaataaa tcttcctata
    36481 acaaaacgca taacatctag gattttatat acctgatatt atgcgttttt aagcacaaag
    36541 acttcttaca caaacttatc tacaattaga ttttatttga catgttttgc caattcttct
    36601 tgggcttttt tattggattc ttctttttct ttcaaccatt tttctctggc ttttgcatat
    36661 tcgtctgttg tgacaatctt atcttgtact ttgaggtatt tatatgattc aacccctttt
    36721 gtaccggtta aaccataggc agcagcaaat ggtacggttc ttctcaatga tggtgttccc
    36781 ccacgcgaaa cacttggaag aactaaagaa ctatcaatca accaagcttg aatatcagca
    36841 tatttctcat aacgtttggc tggatcttgc tctttattag cttcttccaa catttgagta
    36901 tagacatcca gtccaactgc cttagccttg tcattggctt ctccaggctc tagtccaaga
    36961 ttttgtagaa ctcctccact attagtatta aaaatatcga gataggctga cgggtcttga
    37021 taatcaggtc cccaaccgcc atgatataaa tcatagtctt tctgagcagc tgtttgagca
    37081 aagtagcctg aactgtcaaa ctcatctgat gttaattgct gaatgtcaat cactacatta
    37141 tcagaaccta aaacagattc aattgattgt ttgatagaac taactccttg tatgcctact
    37201 ttatctgtta cttccacagt cttatccaag tggattggga attgaacacc ctttgcttcg
    37261 agttctttct tagcttccgc aaacttagcc ttggctttct caggattgta gtaagggtct
    37321 tgaccatccg caaagttgat accttgccat tccttaccat agttgaccat cttagaggct
    37381 acaacttcac caaagtcttt tcccttgata ctgacaaagt ttggaggaac cactaggtta
    37441 cgcaaaatct ttgttgcacc ttctttccct tcagactgag ccccataaga tgttctgtca
    37501 aaagcaaaat tgatagcctg acggaagttt ttattgagaa ctgcttcctg ggtcgatttc
    37561 ttttcaatgt cacttgtttt agaagtataa ttgtaagact tcctatctag gttaaaatta
    37621 aagaaatatg aagttgaatt ttgcatacta tagatgatat tgtttttgta tttttcttta
    37681 atcccttcat agctggagct gttaggaaaa agacgagccg tagtataagc accagctgta
    37741 aaattacgtt ccagtgattc ttggtcgcta ccatcatagt aggtcaattt cacatcgtct
    37801 acaaagacat tcttagcatc ccagtaatta gggtttttct tatattcaat agcagatttt
    37861 gagacaagtg ctttcatcaa gaaaggtcca ttgtacaaaa tactagatgg atccgccttc
    37921 ccaaaatcat ccccttttga tttcaggaaa tctgcattaa caggaaaaag tatcgttgca
    37981 agtgtttttg aattccagta aagttctggt ttaaccaaag tatattgaac cgtttggtca
    38041 tcaagtgcct tgacaccgac agttgaaaag tcgcttgttt taccagtgat atagtcatcc
    38101 aaaccagcaa cagagtcctg cactagatac aaagcttctg attttttatc agctgcatat
    38161 tgcaaacctg tcacaaaatc ctgggcagtt acaggcgcat attcttctcc ctcagaagta
    38221 aaccacttgg catccttacg aagtttgtag gtataggtca aaccgtcctg agaaacagtc
    38281 caatcctctg ctaatgatgg aataatattc ccatattggt cattttctaa taacccgtct
    38341 accaaatttg caacaatatc ggatgttgct gcgcggtttt ctgctagata gttcaaacta
    38401 gatggatcac ttgaataaac atagttatag gtttttgatg cagtgctaga atttccacac
    38461 gcgctcaata aaactcctgt acccaggaca agacctgcca aggttagata tttgctctta
    38521 gactttttca tttccagaac ctcctgcttt aaatatttgt tttattatac aactcttggt
    38581 ttgtttagtc aattgttttt tccaaaaaat caaaaaaacc tcattttcta tttttgaaag
    38641 ataattttag ctattttgac gctaaaaaag gaataagttt caagttctcc taaaagattg
    38701 ttcaacttat tccttttatt tataaatgct accttacgaa agcttaaatt ttgctcgcag
    38761 gcgatctgct tgggcttttc ctattacctt atctaataaa taggtacgca gactcataac
    38821 catataaagt ccacccccca tggcaccgac aagagctaca taaaagaagc tccacaaacg
    38881 tccacttggt tggaagaaaa atcctaacag ccactggatg gttcctatta acagaaacat
    38941 gactagggtc agcaaactga ttaaaatggt tcgcttcaaa atcaccttgc gcttgacacc
    39001 agttacttta caaatatccc gatacatcaa gacgttagga atgatgagag caatggttgt
    39061 tgaaatcaaa ggaccataac tgtggaagag ggcgatggta ggtagttgca agactagctt
    39121 ggcaatagaa ccatagataa aatagagaac ggccttgcgg ttgcggaaca tggcctgaag
    39181 cattggagac aagaccatgt acaagcctaa aataatagac tgcaaaactg caaagacaaa
    39241 taagcccaga gccaaactat ctggcttacc atagaagacc gtataaagag gttctcctac
    39301 cataaccact ccaaccgttg ctggtagcaa gaacataaag agtagggtga gactgtcctg
    39361 aacgagacga gaagccgctt tcaagtcccc cttgacatag ttttccgtca aaagtggcaa
    39421 accaacactc ccaatcgaaa cccctacaga aatcaaaatc atcgtgattt tattaggatt
    39481 ggctgagaaa taagaaaaca tgacaaccaa gtcctcattg ctgtagttgg taaaccagct
    39541 catactattg ataaaggtca gctgatccaa aatctggaag agctggatgg cagaccctgt
    39601 caggataaaa ggaatggctt ccttaatggt atcaaccaag agacgcttac tgttaatctt
    39661 atctcctgtt tcaaagactc ttttgagtga accttcttgg gcaaggaaat aaatcaagac
    39721 tgcaaaactg gctaccatac cgacaaaggc agcaaaggtt gattgggtaa cggctgctag
    39781 ataatctcct gaaccgagct tcataatgat aaaggttgct aggagcatcc agataacacg
    39841 aatgacctgc tcagcaattt ggctcatggc atagggtttg aggttattca tcccttggaa
    39901 aaatcctcgg ataacactca tagacgggaa aatcaagact ccccaagcca agctttgcat
    39961 gattgggatc aagtctttgc ccacgccaga caagtctgct agccaaggag caaagacata
    40021 caagactaaa gcaaaaacca ggcctagtcc tgtcataaag cctaagaagc tccgaatcag
    40081 ggcaaagcta tgctcttctt ctcgcatggt attatacttg gcaacttgct tggccaccgc
    40141 aactggaatc cccgctgttg aaaccaacaa gaaccaagca tagatattgt aacccatggt
    40201 aaagagacca tttgccttag ctgcataagc ccccatccag atgtaccaag ggataatgta
    40261 aacagccccg agtaggcgac tgataaagtt actagccgtt agccaagcag tcccccgtaa
    40321 catctgggcc tgctggtgat tgttttcgtg cgacatagat tcctcattca attttgataa
    40381 ctaggaaaaa ctccttatct tccattataa acttttcctt gttacttgta aatttacgac
    40441 tttcggttta caatagaaag tatgattaag attgaaaccg tattagatat tttaaagaaa
    40501 gatggccttt ttcgcgaaat tattgaccaa ggtcattacc actacaacta cagcaaagtt
    40561 atttttgata gcatcagcta cgacagccga aaagtaacag aagacactct tttttttgca
    40621 aaaggcgctg cctttaaaaa agaatacctt ctttctgcta taacacaagg tttagcttgg
    40681 tatgtagctg aaaaggacta cgaagtcgat atccctgtca tcattgtgaa cgatataaag
    40741 aaagccatga gtttgattgc catggagttc tatggtaatc cacaagagaa actcaaactc
    40801 cttgccttta ctggtactaa gggtaagaca acagcaacct atttcgccta taacatctta
    40861 tctcaagggc atagacctgc tatgttgtcg accatgaaca caactcttga tggcgagact
    40921 ttctttaagt cagcgttgac aacccctgag agtattgacc tctttgacat gatgaatcag
    40981 gctgtgcaaa atgaccgtac tcacctcatc atggaagtct ccagtcaagc ctatctagtc
    41041 catcgagtct atggactgac ctttgatgtg ggagttttcc taaatatcag cccagaccat
    41101 atcggcccga ttgaacaccc tagctttgaa gactatttct accacaagcg tctcttgatg
    41161 gaaaatagcc gagcagtcat cattaacagt gacatggacc acttctcagt cttgagagaa
    41221 caggttgaag atcaagacca tgatttctat ggtagccaat ttgataacca aatcgagaat
    41281 tccaaagcct ttagcttttc agctacgggt aaactcgctg gagattatga tatccaactc
    41341 attggcaact tcaaccaaga aaatgcagtt gctgctggac ttgcttgtct ccgtctcgga
    41401 gcaagtcttg aggacatcaa aaaaggcatc gctgcaaccc gcgttcctgg tcgtatggaa
    41461 gtcctcactc agaaaaatgg agccaaggtc ttcatcgact atgcccacaa tggggatagt
    41521 ctgaaaaaac tcatcaatgt ggttgaaact catcaaaccg gaaagattgc tctggttctg
    41581 ggatcaacag gaaacaaggg agaaagtcgt cgtaaggact ttggcctcct cctcaatcaa
    41641 caccctgaga ttcaagtctt tctgactgct gatgacccta actatgaaga cccaatggcc
    41701 attgcagatg aaattagtaa ctacatcaat catcctgttg aaaagattgc ggatcgccaa
    41761 gaagccatca aggcggcaat ggctatcaca aatcacgaat tagatgcagt tattattgcg
    41821 ggtaagggag ccgattgtta ccaaatcatc cagggcaaga aagaatccta cccaggagat
    41881 acagccgtcg cagaaaatta tttataagaa tagaaaaaat caagggaaaa tgaaaccctt
    41941 gattttttcc tattctttag taaaaacgtt tttcaaacct tcaacggcac cttctacagc
    42001 accttttgcg tcttctacaa cgtcttttgc cttagcaact gttttttcaa cagctccttc
    42061 aagttctgtc ttgctgtctc cagtaacttt accaaaacct tctttgacag cgcctgttgc
    42121 ttgttccaat ttgttttcaa gtgacatatg attgtctcct ttagtttatt ccgatatgtg
    42181 ttaccggtta catatagttt ataccgaata ctaaggaaag tcaaacaatg tgctcagaaa
    42241 caagaaatca cgctaagtag aaagttaatc tctccttatc aaaatcgata gccaactcat
    42301 actttccatc ttcattttgc acaatatagc ctaagacaac caaactgtcc acaaagatat
    42361 cacggcattt ctgcataagc tggtattttt tgagaaattt caacaaaaaa gccgtcatat
    42421 acttgagggc atactcagga ttaacatctc ctaaaatgtc atagagctcc tgctgttttt
    42481 ctgtcaaagg atactgctgt ttgaccttgt aaaaataatt ggacagggtc attttaatgc
    42541 gcgcaaagtc cgtcttttca actaaaatag ctgcattgat ttgattgcgc aattccgtct
    42601 caaaactctg ctccaacaag gcttgataga ccggactagc ttctctgaca aaaatctctt
    42661 gatccagttc aagactatca agtgattcaa gcgtaggaaa attgaggtaa tagcgcttat
    42721 tttcacgtag aatcaagcct gcctttatat actcttccat gagtttatca actgcaacat
    42781 ctggaaattg agccttaatt tcccgtagaa tcacatcctc atactggtcc agatagcgga
    42841 tcaattctcc aaaaatggct gtctcgtcaa acgagatgga ttaaaaatct gaatcatgaa
    42901 gattcctttc tttacattgt aacagaggcg atgctgccaa ctgactagga ttggtaccag
    42961 cttggttcag aggaacaggc agacctagtt ctatgtaata ctccctccaa aaatcactaa
    43021 tctcctgctc cccatctcca aatttcatag gaatagtttc aaaaataggt aggatttctg
    43081 ctatatactg ggagcagtag aaaccagctg catctggata gaaagaagca ttgtagggtg
    43141 ctccaagatg tttgcaagct ctttccttca ccgactggat atccatttct gggtaaacat
    43201 agaggtcgta taaatgattg gactcaaaga agtctgccgg ttcttgacag acaacaccag
    43261 cctgtccact agcatgataa atcatcccat ccaaataaat ggcaacatgg ctatagttac
    43321 ctgtggaagt ctggatggcc tgtcccatgt ctgacccatc tctcacaaaa atcaaatcgc
    43381 cattttctaa catacttcac tcctagaaaa aaggagccga aactcctttc gatataaggc
    43441 aaactagact ttccgatttt gagctgacac ccaaaatctt acgcattaaa gctttcagtc
    43501 aattgaggta ccacttgttt cttacgtgaa acggcaccag caaggaaggc atgattgttt
    43561 tcaagtttga agttgaaagc cgcttcgacc ttgtccatat tggcaccaag agccaagatt
    43621 tctgagtttg agttgacgat atctgtaatc atcaagacaa agtcagagta gccgtttgat
    43681 tcgttggcag cttgcattgc agcttcaatt tctgcttggc gttccaaaac ttcagcgatg
    43741 tcaactgtgt tcacttgggc aacacggaca ttatttccgt tgagttcaaa agttttagca
    43801 tcgatgtcaa tcaattcttc agcagatttg ctagccaagt tggtaccagc tttcaacatt
    43861 gccaaaccat attcttccaa gttcacacca gccaattcag ccaattcagg agcaatgatt
    43921 ttatctgttg ggtgtgttgt tggtgatttc aaaagaaggg tatctgaaat caaacctgaa
    43981 agcatcaaac ctgcaatctc tttaggcaca gctacaccat gttctttgaa catacggtaa
    44041 acgattgaag acgctgatcc aactggctcc aaacgcatgt aaagtgggct tgcagtttca
    44101 aagttagcca cacggtggtg gtctacaaca ccgtaaactt ctacttcagc gatatctgat
    44161 acagattgtt ggaattcatt gtggtcagtc aagataactt gctctgcccc ctctgctttg
    44221 gcagaagtga taacacgtgg tgcttccaca ccaaaatagt tcaagacaaa ggctgtttct
    44281 tcatttggag ttccaagggc aacagcttcc gtatccaaac cgtaagcttc ttttgcaagg
    44341 taggcaaaag ctacagatga cccgatggca tctgagtctg gattttggtg accaaatact
    44401 agaatcttgg acatgataat acctcatttt gtttattttc tttattgtag catttttttc
    44461 gctttttgac aaaggtaaga ggagtcaaag gactccccac gattcttcaa aataactgag
    44521 taaatttcta tcttgatttt cagataaaaa ttcttccttc tgtggcctct tcttacgctt
    44581 gagaagagct tcagccgaca tggcttcttc cttactggca aaaccttgag catagataag
    44641 tttgactggc aagcgtgctc gtgtatattt ggctcccctc ccactattgt ggatagcgag
    44701 gcgtcttctc acatcagtcg tatagcctat atagtaggat ccatcacgac actccagaac
    44761 gtacatataa gccttatgat ccataataaa tctcttcgat ttcgggcgta taagagccat
    44821 catcattgtg gacaatcaaa ggaggtaaga ccttaaagcc acttgttgag ccatccttga
    44881 tcgcctcaat caaaagcata ttggcttcct tttctctttt tggataaaca aactgcaggc
    44941 gcttaggggc tagattatgt cgttttaacg tatccaaaat atccagaagt cgatcaggac
    45001 gatgaaccat ggccaaacgc ccattagact tgagaatact ctgagcacta cgacagattt
    45061 cttccaaatt agtcgtgatt tcgtgtcgag ccaagagata atgttcactc tcgttcagat
    45121 tagaataagg atccaccttg aaatagggtg gattacacaa aatcatatcc accttactcc
    45181 cctgaatgtg agcaggcata tttttcaaat catcgcagat gacctgcatt tgctcctcta
    45241 atccattcaa acggacagag cgttcagcca tatccgccaa acgctcctga atctcaacag
    45301 acaatatctg tgcttgagta cgagtgctag caaaaagccc cactgctcca ttcccagcac
    45361 agaaatccac aatcaacccc ttcttaggaa aacgtggaaa tcgtgataag agaacactat
    45421 ccaccgaata gctaaaaacc tctctatttt gaatgatttt gatatctgtc gaaaagagct
    45481 ggttaatgcg ctctcctgat tttaataatt gttcttcttc catggtccta ttatagcaaa
    45541 tttatattaa cattacaaaa aatataaaac tctaaactac ttcttctttt ttaaatggtg
    45601 cagggcttct ccagtccaga ttggtaacat tcgtcgaaag ggagcaaaac cgtagttaaa
    45661 gcggtcgctt gaaaagcgtc tccgtctagg aaactggtac ttttcttcct ccaaagtgcg
    45721 gatagaaaga ctggctttcc ccgtaaattc atctaaatcc actacctgaa cttgaacctc
    45781 ttcatcgact ttcaaggttt catgaatatt ttcaataaat cctgtccgaa tttctgaaat
    45841 gtgaatcagc cccgtatcac ccgtctctaa ctcaacaaag gcaccgtagg gctgaatccc
    45901 tgtaatacgc ccctttagct tatcaccgat tttcatctta gtcctcgatt tcaatagttt
    45961 caattacaac atcttcaact ggcttgtcca tagctcctgt ctcaacagca gcaatggcat
    46021 ccaagacagc gtaagatgct tcatcagcta actgaccaaa aaccgtgtga cggcggtcta
    46081 ggtgaggtgt cccaccttga ttggcataga tttctgcaat cggttctggc caaccaccac
    46141 gagtaatttc tttcttcgaa taaggtaggt gttggttttg cacgataaag aactggctgc
    46201 cgttggtatt tggaccagca tttgccatgg aaagagcacc acggatattg taaagctctt
    46261 ctgagaattc atcctcaaaa gattcgccgt agattgactc gccacccata ccagttccag
    46321 ttgggtctcc accttggatc ataaagtcct tgataatacg gtggaaaatg acaccatcat
    46381 agtagccatc ttttgaaaga gatacaaagt tagccactgt tttaggagca tgttcaggga
    46441 aaagcttgat acgtaagtct ccgtgattgg tcttaatagt cgcaagagga ccttctactg
    46501 tttcaatgtc tacttgtgga aaatgcaatt ctttttctac cataccaaat acttctaagg
    46561 catcaaaaat gccatcttct tctaatgttt ttgtaatata atccgctttt tctttgattt
    46621 tatcatgaga aattcccatg gcaacgctga ttccagcata atcaaagagt tccaagtcat
    46681 tgagaccatc tccaaaaacc atgaccttct ctggtttcaa gccaaggtgt tccacaacct
    46741 tttccacccc cgtcgctttg gagcctgaaa tcggcacaat atcagacgaa tgttgatgcc
    46801 aacgaaccat gcgaagtttg tctgagagac tgtcaggcaa gtgcaagtca tctcccttat
    46861 cttcaaaagt ccacatctga tagatatctt ctttttcatg gaaatcggga tctacatcta
    46921 agtcgggata aattggattg atagcttcac tcatcatatc ggtgcgagtc gacaacttgg
    46981 catcatgact cccaaccaag ccatactcaa ttccttcttg cttagcccaa gagatatact
    47041 cctcaacatc tgacttttca atctgatgct gataaatgac ctgacctttt ttatcttcga
    47101 tataagctcc attcaaagtt acaaaaaagt caggcttgag atcacgaatc tctggaacaa
    47161 caccaaaaat gccacgtcca gaggcgattc ctattaaaat tcctttttca cgcaactgtt
    47221 taaaaacagt gggaattgta gttggaataa accctgtctt tgaattccgc aatgtatcat
    47281 caatatcaaa aaagacaatc ttgatcttct ttgccttgta tcttaatttc gcgtccatct
    47341 cactacctct ttcaatctaa ctctttccat tatatcataa agtaggcaaa tcccctattt
    47401 tcaaaaagtt tatcattttt attttaattt cttggatgag aaaagagaca tatttatgaa
    47461 aaagctccat cgtgctttta atgtgttctc ttgttttcaa actcgtaaaa agggagccac
    47521 tgagcctaac tcgctctctc atttcaaagc ttgtgaaaaa agacccgttg gggtcttaat
    47581 tcgctttctt gttttcaagc tcatgaaaaa gagacccaac cgggtctttt ctttaatctt
    47641 cgtttacgaa aggcatcaaa gccattacgc gagcgcgttt gatagctgtt gttactttac
    47701 gttggttttt agctgaagtt cctgttacac gacgaggaag gattttccca cgttctgaaa
    47761 cgaaacggct aagaagctca gtatctttgt aatcaacata ttcaattttg tttgctgcga
    47821 tgtaatcaac ttttttacgg cgtttgaatc cgccacgacg ttgttgagcc atgttttttc
    47881 tcctttataa gtttagttgt ccattagaat ggtaaatcat catctgaaat atccaatggg
    47941 tttgttgctc caaatggatt ttcattacgt gaaaagtctg gtactgaatt tgtaggtgct
    48001 gaatagtttg cagttggtgc agagtaagct ccacctgtgt gaccctcacg cacactacgg
    48061 ctttccaaca tttggaaatt ctcagccacg acctctgtca cgtagacacg ttgtccttgc
    48121 tggttatcgt aactacgagt ctggatacga cctgtcaccc cgataagtga gcctttttta
    48181 gcccagttag caagattttc agcctgttgg cgccacataa cgacattgat aaaatcagcc
    48241 tcacgttcac cattttgact cttaaatgta cggtttactg caagagtaaa agtcgcaact
    48301 gctacatttg atggggtata acgcaactca gcgtcacgtg tcatacgccc tacaagtaca
    48361 acattgttaa tcatagttta ccttcttacg cgtcaatttt gacgatcatg tgacgaagaa
    48421 tgtcagcatt gatttttgaa agacggtcaa actctttaag agctgcatcg tcatttgctt
    48481 caacgttaac gatgtggtaa agtccttcac ggaaatcttg gatttcgtat gcaagacgac
    48541 gtttttccca agtttttgat tcaacaacag ttgcaccgtt gtcagtcaaa atagagtcaa
    48601 aacgtgctac caaagcgttt ttagcttctt cttcaatgtt tggacgaatg atataaagaa
    48661 tttcgtattt agccattgat atgttcctcc ttttggtcta atgaccccaa gactttgcaa
    48721 ggggtaagtg aggttcgctc acaataaact attatactag aaaaaatttt tttacgcaag
    48781 taaaaacact agaattcgaa aaaacgccac atgggcgttt tcctgttctt atggtttgat
    48841 acggtgcaac atacgtggga atggaatagc ttcacggata tgttttgttc ctgctgcgaa
    48901 ggttaccata cgttcgatac cgataccaaa tcctccgtgt ggaactgtac cgtatttacg
    48961 aaggtcaagg tagaattcat attctgtacg atccatgcca agttcatcca tcttagcgac
    49021 aagggcatcg taatcttcct cacgcataga cccaccgata atttctccat agccttctgg
    49081 agcaagcaag tctgcacaaa gcacgcgctc tggatttcca ggaactggtt tcatgtagaa
    49141 ggccttgatg gctgctggat agttcatgac aaatgttggc acaccaaagt ggtttgaaat
    49201 ccaagtttcg tgtggtgacc caaagtcatc accatgctca agatgctcgt agtcagcatc
    49261 ttcatcattt tcatgctctt gcaagaggtc aatggcttga tcgtaagtga tacgtttgaa
    49321 tggctctgca atgtagcgtt tcaagagttc tgtatcacgt tccaaggttt ccaaggcttg
    49381 aggcgcgcgg tcaagaacac cttgtagaag agctttcaca taagcttctt gcaagtcaag
    49441 cgactcatca tgtgtcaagt atgagtactc agcatccatc atccagaact cagtcaagtg
    49501 acggcgtgtt tttgattttt cagcacggaa aactggacca aagtcaaaga cacgaccaag
    49561 agccatagcc cctgcttcta ggtaaagctg acctgattgg ctcaagtagg ctggcgttcc
    49621 gaagtagtca gtttcaaaga gttctgtaga atcttctgcc gcatttcctg aaagaattgg
    49681 gctgtcaaac ttcataaaac cattcttgtc aaagaactca taagttgcat agataatagc
    49741 gttacggatt tgcaacacag ctacttgctt acgagagcgt agccacaagt gacggttatc
    49801 catcaaaaag tctgttccgt gttcttttgg tgtgattggg tagtcttgag attcaccgat
    49861 cacttcgatg tctgtgatgt ccaactcata gccaaattta gaacgttcgt cctctttgac
    49921 aatacctgtc acataaacag acgtttcttg gctcaagcgt ttgataacat caaacttctc
    49981 aagtcccact tcttcaccaa atttttcgac aaagtttggt ttaaaagcca caccttgaaa
    50041 gaaggctgtt ccatcacgca attgtaagaa agcgattttc ccttttcctg atttgttggc
    50101 aacccaagcg ccaatcgtca cttcctgacc aacatagtct tttacatcaa taatcgttac
    50161 acgttttgtc attatttttc cttttctttt ttattcttta tggcaaacca cctctatatt
    50221 gttcccatcc aggtcaatca taaaagcagc ataataaatc ggatgctcac ttcgataacc
    50281 aggaacccca ttgtctcgcc cacctgcctc taagccagcc tcataacaag cctgaacttc
    50341 ttccttattt tctgctaaaa aagcaaaatg aacaggatct tgtgttccct gagtcagcca
    50401 aaaatcacca ccaggatgag ggctgttcgg ggatagaaaa ctaattagag aactagtctt
    50461 aaaagccaat ttatagtcca aaggagcgag aaaactccta taaaatcctt ctgaaatttg
    50521 taaatccttt accttaatct caaaatgatc aatcattctc actacccata aatgctttca
    50581 agcgttcgac tgcttcttta agcgtgtcta ggtctgtcgc atagctgagg cggacatttt
    50641 ctggtgctcc aaatccagct cctgttacca aggccacttc ggcttcttct aagatagcag
    50701 ttgtaaagtc tgtcacatcc gtgtagcctt tcatctccat ggcctttttg acatttggga
    50761 agagatagaa ggccccttgt ggtttgacca cttcaaatcc tggcacctct gcaaggaggg
    50821 gatagatggt attaagacgt tcctcaaagg cctgacgcat gctttctaca gtatcttgct
    50881 cacctgatag agcctcaact gctgcatatt gggctactgc tgacggattc gaagttgttt
    50941 gacctgcaat cttggacatg gcagcgataa tgtctgcttc tccaacggca taaccaatcc
    51001 gccaaccagt catggcatag gttttagaca caccattgat gaccactgtt tgcttgcgaa
    51061 tcgcttccga taggctagaa atcggtgtga actcatgacc attataaacc aagcggccat
    51121 agatatcatc tgctaggatg agaatatcat tttctacagc ccagtttcca attgccaaga
    51181 gttcctcacg ggtgtaaatc atacctgtgg gattagatgg cgaattcagc accaaaacct
    51241 tggtcttgtc agtgcgagct gcttctaact gctctacggt caccttaaag tgattgtctt
    51301 ccttagcaga aacaaagacg ggaacgcctt ctgccatctt gacctgatct ccatagctaa
    51361 cccagtatgg ggttgggatg atgacttcat cacctggatt gaccacagcc ataaagaagg
    51421 tatagagaga atatttggct cccgcagcga ctgtcacttg atttgacgct acagaatagc
    51481 cgtaaaagcg ctcaaagtag ctattgacca ccgccttaag ctctggcaga cctgaggtta
    51541 ctgtataaaa agaagcacgc ccatctcgaa tcgatgcaat ggcggcatct tggatatttt
    51601 tgggagtagt gaaatctggc tcacccaagg ttagagacaa aatatctcta ccctcagcct
    51661 tcagtgcttt ggcacgggct ccagcagcca aagtcacact ttcttccatt tctaaaacac
    51721 ggttggatag tttcataggc cctccttatt gaccaatgct cctgtttcaa aatctactag
    51781 ataaaaatca gatcctgact taacttccca gattggcttg tcttgataac gaccaaaggt
    51841 tatcttgtca atctcgccag ctcccttttc cttagaaacc gtttctgctt tttcttgtga
    51901 aataccctga tttagctgat aaacgtaaat cttatggtca tctttaccaa tcaggacagc
    51961 aagcgcttct tgctgtttat tacgaccaag aacgctgtaa taagattcca agccattgta
    52021 taaatcaacc tgatcagcct tctctaatcc tgcatactgc tgagctaatt tttctccttc
    52081 acttttagct gtttgatagg gtttcatgct aagagaaacc atatacagaa aggaagcact
    52141 gataaccaca aacaaaatcg tcatccctag accatactgc cacagtagat tattttttgc
    52201 tttgttttgt ctttttttca ctcgtctatt ttaccatcta ttaagcttta ttacaagtga
    52261 atataagaa
//
LOCUS       DBERCA010000009        50269 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.009, whole genome shotgun sequence.
ACCESSION   DBERCA010000009 DBERCA010000000
VERSION     DBERCA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 50269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.009"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..663
                     /locus_tag="ACL719_000597"
     CDS             <1..663
                     /locus_tag="ACL719_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0698612.1"
                     /translation="DYVLKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGS
                     IEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGL
                     SNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQ
                     ITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYS
                     NN"
     gene            687..2426
                     /locus_tag="ACL719_000598"
     CDS             687..2426
                     /locus_tag="ACL719_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS0698613.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            2477..3244
                     /locus_tag="ACL719_000599"
     CDS             2477..3244
                     /locus_tag="ACL719_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGS0698614.1"
                     /translation="MSQKLNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRMSSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     VGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3246..3878
                     /locus_tag="ACL719_000600"
     CDS             3246..3878
                     /locus_tag="ACL719_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698615.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLTG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            4019..4297
                     /locus_tag="ACL719_000601"
     CDS             4019..4297
                     /locus_tag="ACL719_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HGS0698616.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(4807..5220)
                     /gene="ndk"
                     /locus_tag="ACL719_000602"
     CDS             complement(4807..5220)
                     /gene="ndk"
                     /locus_tag="ACL719_000602"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGS0698617.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(5331..9008)
                     /gene="rpoC"
                     /locus_tag="ACL719_000603"
     CDS             complement(5331..9008)
                     /gene="rpoC"
                     /locus_tag="ACL719_000603"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGS0698618.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(9041..12652)
                     /gene="rpoB"
                     /locus_tag="ACL719_000604"
     CDS             complement(9041..12652)
                     /gene="rpoB"
                     /locus_tag="ACL719_000604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS0698619.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            13068..13138
                     /locus_tag="ACL719_000605"
     tRNA            13068..13138
                     /locus_tag="ACL719_000605"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13100..13102,aa:Cys,seq:gca)
     gene            complement(13336..14667)
                     /locus_tag="ACL719_000606"
     CDS             complement(13336..14667)
                     /locus_tag="ACL719_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGS0698620.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(14958..15782)
                     /gene="endA"
                     /locus_tag="ACL719_000607"
     CDS             complement(14958..15782)
                     /gene="endA"
                     /locus_tag="ACL719_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGS0698621.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15821..16009)
                     /locus_tag="ACL719_000608"
     CDS             complement(15821..16009)
                     /locus_tag="ACL719_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS0698622.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(16002..17285)
                     /gene="murA"
                     /locus_tag="ACL719_000609"
     CDS             complement(16002..17285)
                     /gene="murA"
                     /locus_tag="ACL719_000609"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS0698623.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(17309..17539)
                     /locus_tag="ACL719_000610"
     CDS             complement(17309..17539)
                     /locus_tag="ACL719_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGS0698624.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17597..18634)
                     /locus_tag="ACL719_000611"
     CDS             complement(17597..18634)
                     /locus_tag="ACL719_000611"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGS0698625.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18618..19106)
                     /gene="coaD"
                     /locus_tag="ACL719_000612"
     CDS             complement(18618..19106)
                     /gene="coaD"
                     /locus_tag="ACL719_000612"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGS0698626.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDMIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(19096..19635)
                     /gene="rsmD"
                     /locus_tag="ACL719_000613"
     CDS             complement(19096..19635)
                     /gene="rsmD"
                     /locus_tag="ACL719_000613"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGS0698627.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19700..20692)
                     /gene="asnA"
                     /locus_tag="ACL719_000614"
     CDS             complement(19700..20692)
                     /gene="asnA"
                     /locus_tag="ACL719_000614"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGS0698628.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20803..21486)
                     /locus_tag="ACL719_000615"
     CDS             complement(20803..21486)
                     /locus_tag="ACL719_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGS0698629.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21542..22282)
                     /locus_tag="ACL719_000616"
     CDS             complement(21542..22282)
                     /locus_tag="ACL719_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGS0698630.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            22316..22594
                     /locus_tag="ACL719_000617"
     CDS             22316..22594
                     /locus_tag="ACL719_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGS0698631.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            22672..23598
                     /gene="yidC"
                     /locus_tag="ACL719_000618"
     CDS             22672..23598
                     /gene="yidC"
                     /locus_tag="ACL719_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS0698632.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23673..24467)
                     /gene="pflA"
                     /locus_tag="ACL719_000619"
     CDS             complement(23673..24467)
                     /gene="pflA"
                     /locus_tag="ACL719_000619"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGS0698633.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(24593..25843)
                     /locus_tag="ACL719_000620"
     CDS             complement(24593..25843)
                     /locus_tag="ACL719_000620"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGS0698634.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25910..26737)
                     /gene="purR"
                     /locus_tag="ACL719_000621"
     CDS             complement(25910..26737)
                     /gene="purR"
                     /locus_tag="ACL719_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGS0698635.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(27004..27945)
                     /locus_tag="ACL719_000622"
     CDS             complement(27004..27945)
                     /locus_tag="ACL719_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGS0698636.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(27935..29191)
                     /gene="rmuC"
                     /locus_tag="ACL719_000623"
     CDS             complement(27935..29191)
                     /gene="rmuC"
                     /locus_tag="ACL719_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGS0698637.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(29193..29855)
                     /locus_tag="ACL719_000624"
     CDS             complement(29193..29855)
                     /locus_tag="ACL719_000624"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGS0698638.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29818..30474)
                     /gene="rpe"
                     /locus_tag="ACL719_000625"
     CDS             complement(29818..30474)
                     /gene="rpe"
                     /locus_tag="ACL719_000625"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGS0698639.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30485..31363)
                     /gene="rsgA"
                     /locus_tag="ACL719_000626"
     CDS             complement(30485..31363)
                     /gene="rsgA"
                     /locus_tag="ACL719_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGS0698640.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31365..32237)
                     /gene="rsmA"
                     /locus_tag="ACL719_000627"
     CDS             complement(31365..32237)
                     /gene="rsmA"
                     /locus_tag="ACL719_000627"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGS0698641.1"
                     /translation="MRIADYRVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32368..32676)
                     /locus_tag="ACL719_000628"
     CDS             complement(32368..32676)
                     /locus_tag="ACL719_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698642.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32669..33310)
                     /locus_tag="ACL719_000629"
     CDS             complement(32669..33310)
                     /locus_tag="ACL719_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698643.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(33312..35342)
                     /locus_tag="ACL719_000630"
     CDS             complement(33312..35342)
                     /locus_tag="ACL719_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS0698644.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYIP
                     SGNVIYVSPRYLIEEKITVSSEFIDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35419..35715)
                     /locus_tag="ACL719_000631"
     CDS             complement(35419..35715)
                     /locus_tag="ACL719_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS0698645.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(36035..36946)
                     /locus_tag="ACL719_000632"
     CDS             complement(36035..36946)
                     /locus_tag="ACL719_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGS0698646.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     EDLFYEQLPNEEKVWFEATRATIDVIRSGRPEYGETVLDDYFKTIYDKELFLINELEV
                     INLYFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIASEYLFALSNTLFSGLACL
                     DNLSSYDSLGAYIFSLNHIMEKTQDFQKKPIILMLEWKLSLIINNDYVSAEQFYQKSK
                     LFADIIENSYLVTMLEKQWQEDLKKYL"
     gene            complement(37018..37578)
                     /gene="rnmV"
                     /locus_tag="ACL719_000633"
     CDS             complement(37018..37578)
                     /gene="rnmV"
                     /locus_tag="ACL719_000633"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGS0698647.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37578..38351)
                     /locus_tag="ACL719_000634"
     CDS             complement(37578..38351)
                     /locus_tag="ACL719_000634"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGS0698648.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELLLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(38656..39408)
                     /locus_tag="ACL719_000635"
     CDS             complement(38656..39408)
                     /locus_tag="ACL719_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HGS0698649.1"
                     /translation="MCPVIILFYYKVEVAGDGTATITFPDGSVVTILGKDTVQQSAKG
                     ESVTQEATPEYKLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASK
                     QLATEKESAKNAIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTM
                     EDVKEAETIGVQAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPN
                     TGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            complement(39584..39718)
                     /gene="rpmH"
                     /locus_tag="ACL719_000636"
     CDS             complement(39584..39718)
                     /gene="rpmH"
                     /locus_tag="ACL719_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGS0698650.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39863..41077)
                     /locus_tag="ACL719_000637"
     CDS             complement(39863..41077)
                     /locus_tag="ACL719_000637"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0698651.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKKLLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(41233..41526)
                     /locus_tag="ACL719_000638"
     CDS             complement(41233..41526)
                     /locus_tag="ACL719_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698652.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            41688..42140
                     /locus_tag="ACL719_000639"
     CDS             41688..42140
                     /locus_tag="ACL719_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGS0698653.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(42175..43563)
                     /locus_tag="ACL719_000640"
     CDS             complement(42175..43563)
                     /locus_tag="ACL719_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0698654.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGKSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43629..44591
                     /locus_tag="ACL719_000641"
     CDS             43629..44591
                     /locus_tag="ACL719_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGS0698655.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(44887..45897)
                     /gene="ccpA"
                     /locus_tag="ACL719_000642"
     CDS             complement(44887..45897)
                     /gene="ccpA"
                     /locus_tag="ACL719_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGS0698656.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(46322..46921)
                     /locus_tag="ACL719_000643"
     CDS             complement(46322..46921)
                     /locus_tag="ACL719_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0698657.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDITILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(46923..48020)
                     /locus_tag="ACL719_000644"
     CDS             complement(46923..48020)
                     /locus_tag="ACL719_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0698658.1"
                     /translation="MLERLKSIHYMFCASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTVFVAGNYIWFLFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     ERVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(48020..48757)
                     /locus_tag="ACL719_000645"
     CDS             complement(48020..48757)
                     /locus_tag="ACL719_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698659.1"
                     /translation="MKRWIALNKIEFLLTKRQLVYYLLSVGMPTAFYLFFSGIYQETP
                     GGPANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVEIPLGSWVGAAILLILGSVAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(48759..49643)
                     /locus_tag="ACL719_000646"
     CDS             complement(48759..49643)
                     /locus_tag="ACL719_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698660.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(49630..49824)
                     /locus_tag="ACL719_000647"
     CDS             complement(49630..49824)
                     /locus_tag="ACL719_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698661.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(49821..50027)
                     /locus_tag="ACL719_000648"
     CDS             complement(49821..50027)
                     /locus_tag="ACL719_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698662.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14660 a        11295 c         9556 g        14758 t
ORIGIN      
        1 gattatgttt tgaaaggaat aaatgttact attaaaaaag gagaaactgt tgctattgtt
       61 ggagaatcag gttcaggtaa gagtacatta gctaaaattt tattaggttt attagaacct
      121 aatattggtt caatagaagt tgatggagta gaaaaagaag aaattggtca aacattgtat
      181 agaaagattt ttggagcagt gttacaaaat tcaaccctaa gttatggtac cttaagagag
      241 aatttgacat ttggacactt tgtttcagat gaagaattaa tgacaaatct aaattcaatt
      301 ggtcttagca atgtagttaa atctttacct cttggattag agacaatcat cgctgaagaa
      361 ggtaataact tttctggagg gcagcagcaa atgatacttt tagctcgttg tcttttgtcg
      421 aaaccttcgg tagttgtttt ggacgaagca acaagtagtt tagataattt atctcaacaa
      481 attacaactt cttacttaag tgaaatcggt accactaaga ttttaattgc ccatcgacta
      541 gatactatca agtctgcaga taagatctta gtaatgcata atggtgaaat tgtagagatt
      601 gggacccata gagaacttct tgaactagga ggcatttata agcaattgta ttcaaataat
      661 tagtttttga ttaaaagggt aaatttatga agattatgaa aaaaaaatat tggactttag
      721 cgatattatt cttttgtttg ttcaataatt ctgttactgc tcaagaaata cctaaaaatc
      781 ttgatggcaa tataactcac actcagacta gcgaaagttt ttctgaatct gatgaaaaac
      841 aggttgacta ttctaataaa aatcaagaag aagtagacca aaataaattt cgtattcaaa
      901 tcgataagac agaattattt gtaacaacag ataaacattt agaaaaaaac tgttgtaaat
      961 tggaacttga accacaaata aataacgata ttgttaactc tgaaagtaat aatttactag
     1021 gcgaagataa tttagataat aaaattaagg aaaatgtttc tcatctagat aatagaggag
     1081 gaaatataga gcatgacaaa gataacttag aatcgtcgat tgtaagaaaa tatgaatggg
     1141 atatagataa agttactggt ggaggcgaaa gttataaatt atattctaaa agtaattcta
     1201 aagtttcaat tgctatttta gattcaggag tcgatttaca aaatactgga ttactgaaaa
     1261 atctttcaaa tcactcaaaa aactatgtcc ccaataaagg atatttagga aaagaggagg
     1321 gagaggaagg aataatatca gatattcaag atagattagg tcatggtacg gctgttgtag
     1381 ctcaaattgt aggggatgac aatattaatg gagtaaatcc tcacgttaat attaacgtct
     1441 atagaatatt tggtaagtcg tcagctagtc cagattggat tgtaaaagca atttttgatg
     1501 ctgtagatga tggcaatgat attatcaatc ttagtactgg acaatattta atgattgatg
     1561 gagaatatga ggacggaaca aatgattttg aaacattttt gaagtataaa aaggctattg
     1621 attacgcgaa tcaaaaagga gtaattatag tagctgcatt agggaatgac tccctaaatg
     1681 tatcaaatca gtcagattta ttgaaactta ttagttcacg caaaaaagta agaaaaccag
     1741 gattagtagt tgatgttcca agttatttct catctacaat ttcggtcgga ggcatagatc
     1801 gcttaggtaa tttatcagat tttagcaata aaggggattc tgatgcaata tatgcgcctg
     1861 caggctcaac attatctctt tcagaattag gacttaataa ctttattaat gcagaaaaat
     1921 ataaagaaga ttggattttt tcggcaacac taggaggata tacgtatctt tatggaaact
     1981 catttgctgc tcctaaagtt tctggtgcga ttgcaatgat tattgataaa tacaaattaa
     2041 aagatcagcc ctataattat atgtttgtaa aaaaaattct ggaagaaaca ttaccagtaa
     2101 aaaatggtat aaaagtgtta aatataccaa acgtattgag atatgatttg aatatgttac
     2161 aattagaata taaaaatgaa caaagttggg atagtttcat agataatgtt aatttaattg
     2221 agttggaaga gagaattcaa actactattg gaattaaaca aataaacaca cacaatatta
     2281 ttactattgc ccgagaaggg tactctcaaa attatttacc taacacttca gaaaatacat
     2341 ataattcatt acaagtcagt ttagttggag tattactact ttttataagt atggtaaata
     2401 ttttatgggc taaaaaaagt aaatgaaaat aaaatttgga gccctctgaa aaagtaagta
     2461 ctacagttca actaaaatga gtcaaaagtt gaatcacctt gatgtagggg agtttgtctt
     2521 attgctgcct gaacacctcc gttcagagga agaacattat aaatctgttt ttgaagacga
     2581 cttaaccagt cgcatgtcta gtcaagatga acgacagcaa atgactgcta cggtaggtta
     2641 tttagaatca ggtcaggatc gttttgtgta taatacgacc cctatttctt accagcagtt
     2701 tttgaaagat ccaatcatca ttgttataac accccaatca actggtccac agtccatttt
     2761 gttttggata gacgcagtac agaactacgt tctctttaat caattgtctg atgcccagga
     2821 gcttatccag agacaaggca ttgaaaattg ggtctcagaa atgcaaacag gttaccacaa
     2881 ctacatcaca ttattggata atatccagag ggaacgttgg gtaatgctag caggagctgt
     2941 gcttgggatt gcaacttcaa tcttgttgtt taacactatg aataggctct actttgaaga
     3001 atttagacgt gccattttta tcaaacgcat tgcaggtctc aggttcttag aaatccatcg
     3061 cacttatctc tttgctcaac tgggtgtgtt tttactggga tttgttgcga gtgtatttct
     3121 tcaggtagag gtaggagttg ctttcttagt cttgttactc tttactggtc tatctctttt
     3181 acagttacat gtccaaatgc agaaagaaaa caagatgtcc atgcttgttt tgaagggagg
     3241 ttaatatgat tgaacttaaa caggtgagta aatcttttgg agaacgagag ttattttcga
     3301 atctttcaat gacatttgag gctggaaaag tctatgcctt aattggttca agtggtagcg
     3361 gaaaaacaac cttgatgaac atgattggga aattagaacc ttatgatggg acgatttttt
     3421 accgaggtaa agacttggcc aattataaat cgagtgattt tttccgtcac gaattgggtt
     3481 acctcttcca gaactttggc ttaattgaaa accaaagtat tgaagaaaac cttaagctag
     3541 gtctcactgg tcaaaagttg agtcggtcgg aacagcggtt gaggcagaag caggctttag
     3601 aacaggtcgg cctggcttat cttgacctag ataagcgcat ctttgagtta tcgggcggag
     3661 aatcgcaacg ggttgccttg gcaaaagtta tcttaaagaa tccacccttt attctggcag
     3721 atgagccaac agcttcaata gacccagcaa cctctcagtt gattatggag attttgctat
     3781 ctcttcgaga tgataatagg ctaatcatta tcgcaacaca taatccagca atttgggaga
     3841 tggctgatga agtgttcacg atggatcatc tgaaataaaa atccttgttt ttaattgcac
     3901 gatgagttac tgaaatatta tcatgaatca ataattggag ttaatttaga attgtacttt
     3961 attaatattg aggtaacttt ttcttgataa aggaagaaat agtggagagg aagttagaat
     4021 gaaaaaattc gacaattata ttattgagaa gccttgcgat tctaattcag ataaactgca
     4081 aaaaatctta ataattgaaa atttggtaga tgatattttg caatttcctc tcagaatcaa
     4141 taatagtgta ggagagattt tcctcctaca accgtttcaa aagaaaacta tctttattcc
     4201 atgttatttt gaggaagata ttgtgaaagt caaagatgat gataaagttg agtggaattt
     4261 gttagaattt caaaaattta gagcattttt ggcttagtaa tctgtgttga aggctcaaaa
     4321 cctatggtaa aaaagtcgct ttgaaaacgt attgcctcca aagatttagt taaataatga
     4381 tttaacacaa aaagaaatta ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag
     4441 gtgggaaaaa cttgcgattt tcacagagaa aggaagaaaa agtatagaaa tatagtcaat
     4501 tgaaacaaga acaggataaa agaacctttt gtgccatatt tttctccttt cgctttacaa
     4561 ttggattgaa cacctttatt gtatcgcgtt tggagttttt ttggtataac cttcgacgcg
     4621 cacccgcata gcgggtgttt tttttgtctc gcacctaacg gagcgagaca aactaatagt
     4681 cacttaatca aaaaacgcac catatcaaaa actaaaaagt ttgatatcat gcgtcatgtc
     4741 ttaaactaat tgactatact ttctattcaa atgagctttt aaccaattga ttgagccaat
     4801 ccactcttaa aaccaaagag caatttctcg cttagctgac tcttctgaat ctgaaccatg
     4861 tacaacattt tggataatct cattttctcc agcagctttt gcaaaatcac ctcgaatagt
     4921 gcctggtaaa gcttcttctg gacgagttgc acccatcatg gtccgccaag tttcgattac
     4981 tttgggacca gaaatgacac ccacaagaac tggacctgaa gtcatgaatt cacgaatcgg
     5041 tgggtaaaaa ctctgaccaa ccaagtcctg atagtgctgg tcaatcaact cttctgaaac
     5101 ctgtgaacga aactccaatt tttcgattgt aaatccacgt tgttcgatgc gctttaacac
     5161 ttcacccact agccctcttt ttacaccatc tggtttgatg ataaagaatg tttgttccat
     5221 acccgtctcc tttgtcagct tctttctttt attttaccac atttcgtgga aaaatggaga
     5281 aagttttcag aagagagaat gagagaaccc gagggttctc tcattctctc ttattctact
     5341 gtttcttcca cagtttcaac ggcagtatcc acaactactt ctgttgtttc ttcatttcct
     5401 tcttcctcta ctggaggatt aaggtattct tcttcgttga cagcatgtgg ttcaaggtta
     5461 cggtaacggg ccataccagt accagctggg atgatcttac cgatgataac attttcttta
     5521 agtccaagga gatggtcttt cttaccacgg atagctgcgt cagtaaggac acgagttgtt
     5581 tcctggaagg aagccgctga caagaaactg tttgtttcaa gtgaggcttt ggtaattccc
     5641 ataaggactg ggcgacctgt cgctggaact ccacctgcga taaggacatc tttgttggca
     5701 tctgtaaagt cattgatatc catgagggta cccatgagaa gatctgtatc acctggatcc
     5761 atgacacgga ctttacggat catttgacga accattacct cgatgtgttt gtcaccgatt
     5821 tctacccctt ggctacggta aactttttgt acttcaccga gaaggtacgt ttcaactgac
     5881 aagacatcac gaactgcaag gagacgtttt ggttggatag aaccttctgt cagagcagca
     5941 ccacgcgcta cttggccccc aacttcgaca cgcatacgag ctgtaaatgg aacgacatat
     6001 tcaccttcgc cagtttcacc cttaacaaag actttcttgg tacgagttga tgcatcttct
     6061 tcgatagcag taacttgtcc tttaacctct gtaataaccg cttccccttt aggattgcgg
     6121 gcttcaaaga tttcttggac acgaggaaga ccctgagtga tatcggtatt tgaggcaacc
     6181 ccacctgtgt ggaaggtacg cattgtaagc tgtgtaccag gttccccgat agattgggca
     6241 gcaattgtac caactgcttc accaacttca accgcatcac cagtcgccaa gttgataccg
     6301 taacagtgac ggcagacacc gtgacgagtg ttacatgtaa atacagaacg gatagtcact
     6361 tcttccacac cagcattgac aatttcacgc gccttgtctt ctgtaatcaa ttcatttgga
     6421 ccaataatca ctgcaccagt ttctggatgt ttaacagttt tcttagtgta acgaccgttg
     6481 agacgctctt cgagagactc gatcatctct tttccttctg cgatagaacg gatcaagaga
     6541 ccacggtcag ttccacagtc gtcctcacgg atgataacgt cttgggcaac gtcgaccaaa
     6601 cgacgagtca agtaacctga gtcggctgtc ttaagggccg tatcggtcat acctttacga
     6661 gcaccgtgag ttgagaagaa catttccaat accgacaaac cttcgcggaa gtttgaaagg
     6721 attggcaatt ccatgatacg tccattcgga gcagccatca gaccacgcat accggcaagc
     6781 tgtgagaagt ttgagatgtt accacgggct ccagagtcca tcatcataac gattgggttc
     6841 ttaggatctt ggttagcaat caagcgtttc tcaagttttt cacgggcagc acgccattca
     6901 gctgtaacag cattgtaacg ctcgtcgtct gtgatcatac cacgacggaa ttgtttggtg
     6961 atttgttcga cacgtttgtg tgattcttca atgatttcag tcttgtcatc aacgactggg
     7021 atatcggcaa tacccactgt caatcctgca agagttgagt ggtggtaacc gaggttcttc
     7081 atgcggtcaa gtagggcaga agtttctgtc gtacggaaac gtttgaagat ttcagcgatg
     7141 atatttccaa ggtttttctt cttgaatgga gggttgagct caagattgct gatagcttcc
     7201 ttgatatctc caccaagtgg caagaagtat ttagctggaa caccttctgt caagttggca
     7261 ttgtttggtt cttgcaagta tggtagcccc tctggcatga tatcgttgaa gagaatttta
     7321 ccaactgttg taagcaagac cttatgtctt tgctcttctg tccaaggctt gttgaggctg
     7381 tctgttgcga taccaacacg tgagtggagg tgaacataac cattgcggta agccataacc
     7441 gcttcgtcac ggtctttgaa gaccattcct tcaccttcgc gaccagcttc ttccatggtc
     7501 aagtagtagt tacccaaaac catgtcctga gatggagtaa ctaccggttt cccatctttc
     7561 gggttcaaga tgtgctcagc agctagcatg aggatacgag cttctgcttg tgcttcttct
     7621 gaaagtggta cgtggatggc catttggtcc ccgtcaaagt cagcattgta ggcttcacag
     7681 acaagtgggt gcaagcgaag agccttacca tcaatcaaga ctggctcgaa ggcttggata
     7741 cccaaacggt gaagggtcgg tgcgcggttc aaaagcactg ggtgttcttt aatcacttct
     7801 tcaaggatat cccagatacg ctcatctccg cgttccacca agcgtttagc tgctttgacg
     7861 ttttgcacga tatcacgggc aacgatttca cgcatgacaa atggtttaaa gagttcaatc
     7921 gccatttcac gtggcacacc acattggtac atcttaagag ttggaccaac ggcgataacg
     7981 gaacgtcctg agaagtcaac acgtttaccg agcaagtttt gacggaagcg tccttgttta
     8041 cctttaagca tgtggctcaa tgatttcaat ggacggctac ctggtcctgt gattggacga
     8101 ccacgacgac cattgtcaat caaagcgtca actgcttctt gaagcatacg cttctcattt
     8161 tgaacgatga tacctggtgc atttaactca agcaaacgag ccaaacggtt gttacggttg
     8221 ataacacggc ggtaaaggtc attcaagtca gatgaggcaa aacggccacc atccaactgc
     8281 aacattggac gaagatctgg tgggataacc ggaaggatgt taagaatcat ccattcaggt
     8341 ttgtttccag acttgtaaaa ggcatccaaa acatccaaac gacggatggc tttgacacgt
     8401 ttttgtccag tagctgtttt caattcttct ttgagttcag caatttcttt ttcaagatct
     8461 acttgcttca aaaggtcttg gatggcttcc gcacccatct tggcaacaaa tgaaccataa
     8521 ccatattcac gcaagcgctc tcggtattcg cgctctgtca tgatagactt gtgctcaagt
     8581 ggtgtatcct taggatcaat caccacataa gccgcaaagt agataacttc ctcgagggca
     8641 cgagggctca tatcaagggt caagcccata cggcttggaa tccccttgaa gtaccagatg
     8701 tgagatacag gagctttcaa ttcgatatgt cccatacgct cacgacgaac tttcgtacgc
     8761 gttacttcaa ccccacagcg gtcacaaaca attcctctgt aacgaatgcg tttgtactta
     8821 ccacaagcac attcccagtc ttttgtagga ccaaagatca cttcatcaaa gagtccttca
     8881 cgttctggtt tcaaggtacg ataattgatt gtttcaggtt ttttgacttc tccataagac
     8941 catgaacgga ctttacttgg agaagctagg gtgatttgca tacttttaaa acgatttaca
     9001 tcaaccacta tttcttccct ttctattcta agtgaactgc ttattcttgt tcagcagctt
     9061 cttctgttgc ttccgctttt gttgctttct cagcttcttc agcttcaaag gctgctttag
     9121 cctcttgggc tgctttttcg cgggcttttt caaggtcatc tacgtggatg acatcttcgt
     9181 ccattccttc atccaagtcg cgaagttcca cttcttggtc atcttcgtct aggacacgca
     9241 tgtcaagacc aagagattgc aattctttga caagaactcg gaaggattct ggaacacctg
     9301 gttttggaat tggtttgcct tttgtaatag cttcataggc tttcaaacgt ccgttgatat
     9361 cgtccgactt gtaagtcaag atttcttgaa ggacatttga cgcaccgtag gcttcaagag
     9421 cccaaacctc catctcaccg aaacgttgtc caccaaactg agccttacct ccgagtggtt
     9481 gttgggtaac agttgagtat ggtccgactg aacgcgcgtg caatttatca tcaaccatgt
     9541 ggtggagttt gatcatgtac atgactccga cagaaacacg gttatcaaac ggttcaccag
     9601 tacgtccatc gtaaaggatc gttttggcat cgctatccat acctgcttct ttaacagttg
     9661 accaaagatc ttcagaactt gctccatcaa agactggtgt cgcgatgtga ataccaagag
     9721 tacgagctgc cataccaagg tgaagctcca taacctgacc gatattcata cgtgatggta
     9781 ccccaagtgg gttcaacatg atgtcgactg gagttccgtc tggaaggtaa ggcatgtctt
     9841 ctacaggaac gatacgagag acaacccctt tgtttccgtg acgtccggcc attttatctc
     9901 cgaccttaat cttacgtttt tgagcgatgt aaacacgaac caacatgtta acacctgatt
     9961 gcaactcatc tccatttaca cgtgtaaaga tcttaacatc acgaacgaca ccatcggcac
    10021 cgtgtggtac acgaagagaa gtatcacgca cttcacgaga cttgtctcca aagatagcgt
    10081 gcaagagacg ttcttcagct gaaagatctt tctcaccctt aggtgttact ttacctacaa
    10141 gaatatcacc ttctttaacc tcagcaccaa tacggataat ccccatttcg tcaaggtctt
    10201 tgagggcatc ttcaccaacg tttggaattt cgcgagtgat ttcttcaggc ccaagctttg
    10261 tatcgcgcgt ttctgattcg tattcttcaa ggtgaacaga tgtgtagaca tcgtccttca
    10321 ccaagcgttc gctcatgata acggcatcct cgaagttgta accttcccaa gtcatgtagg
    10381 caacgattgg gttttgtcca agcgccattt ctccattttc catagaaggt ccgtcagcga
    10441 tgaaatcgcc tttttcaacg acatcaccaa cttttacgag agtgcgttgg ttgtaagcag
    10501 tacctgagtt tgaacgacgg aatttttgga tgtggtaaac atccaatgaa ccatcttcac
    10561 gacgaacttc taccttgtca gcatctgcgt aagtaacttt accatcatac tgagcaatca
    10621 cagccgcacc agaatcgtgg gctgcttggt attccatacc agtaccaacg taaggtgcct
    10681 gaggattaat caatggcaca gcctgacgtt gcatattggc tcccatgagg gcacggttgg
    10741 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactacc tgttttggtg
    10801 aaacgtccat gtagtcaaca atattagctg gatactcttg gttgacccct tggtgacgtc
    10861 ccatgacaat cttctcagca aaggttccat cttcattcag acgagagtta gcctgagcta
    10921 cagtatattc atcttcttca tcagctgtca accaaacaat ttcgttcgtg acaacacctg
    10981 tttcacggtc aaccttacgg tatggtgttt gaacaaaacc atatttgttc aagtgtccgt
    11041 aagatgacaa gttattgatc aaaccgatgt taggtccttc aggtgtctcg attggacaca
    11101 tacgaccata gtgagtgtag tgcacgtcac gtacttcata tccagcacgg tcacgagtca
    11161 aaccaccagg tcctaaggct gacaaacggc gtttgtgaga caactcagaa agcgggttgt
    11221 gttggtccat gaactgtgac aactgtgatg aaccaaagaa ttctttaact gcagctgtta
    11281 caggacggat attgataatt tgttgtggtg tcaagacttc attgtcctga acagacatac
    11341 gttcacggac attacgttcc atacgagaaa gtcccaaacg tacttggttg gcaagcaatt
    11401 caccaaccgc acggatacga cgatttccaa ggtggtcgat atcatctaca cggccaagtc
    11461 cttcagccaa gttgaggaag tagctcatct cagcaaggat atctgcagga gtcaccgtac
    11521 gaaccttgtc atctgggtta gcattaccaa tgatcgttac gacgcgatct ggatcagttg
    11581 gagcaataac cttgaatttt tgaagaacaa caggctcagt cacaacggct gcatcgtttg
    11641 ggatgtagac aatcttgttc aagtcgccat ccaaatggct ttcaatgctt tcaatcacgc
    11701 tacgagtcat aatcgtacca gcttctacca agatttctcc agtttcaggg tctaccaatg
    11761 gctctgcaat ggtttggttg agcaaacgtg ttttaacatt gagtttttta ttgattttgt
    11821 aacgaccaac tgctgccaag tcatagcgac gtgggtcaaa gaagcgagcc acaagcaagc
    11881 tacgtgagct ttcagccgtc ttaggctcac ctggacgaag gcgttcgtaa atttctttca
    11941 aggcttcgtc tgtacgagag tccattggat tcttgtggat atctttttca acagtgttgc
    12001 gaaccaattc gctgtcacca aaaatatcaa agatttcatc atcacctgag aaaccaagag
    12061 cacgaaccaa ggttgtaaat ggaatcttac gagtacggtc gatacgagtg taggtgatat
    12121 cttttgagtc gctttcaagt tccaaccaag ctccacggtt agggataaca gttgaaccat
    12181 agcccacctt accatttttg tctactttgt cgttaaagta aacacctggt gagcggacca
    12241 actgagaaac gataatacgt tcaccaccat tgatgatgaa agtacccatt tctgtcatga
    12301 ttgggaaatc accaaagaaa acttcttggg tcttgatttc gcctgtttct ttattgatca
    12361 agcggaaggt tacaaaaatt ggtgctgagt agctagcatc gtgaatacga gcttcttcta
    12421 gcgtgtattt tggttccttg atttcatatc caacaaattc caactccatt gtgtctgtga
    12481 agtttgaaat tggcaataca tcttcaaaca cttccttaag accgtggtct aggaaagctt
    12541 tgaatgagtc agtttgaatt tcaatcaaat ttggtaagtc aagaacttct ttgattcttg
    12601 aaaaactacg acgggtacga tgtttcccgt attgaacgtc atgtcctgcc aagatgattc
    12661 tcctttgtaa ataagttcca agccttgtca atcaggcttt tctaatcgcc atatggttgt
    12721 aaacccctta tcaccgtgtc ctcttgacga attttcagaa tctttaagcc tctgttacaa
    12781 atgctcaaaa tcttgaaaaa aagcacaaaa agagcagcta aatctgactt tttcagaaga
    12841 tttaactgct gtgagccttg tctggacaat atttcagaca aaacctacga caaatgatta
    12901 cccatattat accctattta gctagatttt tcaaggggat tcagtaggtt tttggtaatt
    12961 tttttcccat agaaaacttg gcatcacatt cgaatcacgc tatggtacaa aaaactgaaa
    13021 aaactattga ctgaaaatca ttttcaaggt ataataataa acgttaaggc ggtatagcca
    13081 agtggtaagg cacggctctg caaaagcttg atcgtcggtt caaatccgtc taccgccttc
    13141 tataacttga tttatcaggt ttcaaatgaa cagaaagccc aatttgaagg gcttttttta
    13201 ttttccctcg aataaatacg tataacttta aaaacttttg gagcgagttt gtggcagagt
    13261 tctttccatg gcataattcc cttttgaaat caattgaaaa aagcttcgtc acgcctggtg
    13321 acaaaagcct ctagtttatt ctgtttcttc ttctatttcg acttctgtga tttggacttt
    13381 taccttggta acacgtccat tcttcacttt atcattggtt aagataaact gtttgttttg
    13441 gctgaccaat tcatagctga gtttctcagt cgttggaatg gttcccactc ctgtcaaata
    13501 ataaccagcg atggtatcaa cgtcatcact ttccagttca acatcaaagt aattattgaa
    13561 atcattgagg gtcatagttc cttgaacaat gtaggtatcc tcgccaatct gatgaacatc
    13621 tattgctgct ttatccgttt catcatcaat ttcaccaaca atttcctcta agaggtcttc
    13681 caaagtcact aaaccagcca taccaccgta ttcatccagc aagattgcca tttgtctttg
    13741 ggtatttcgc agttctttta gcaagtcatc cacaaaaata gtttcaggta caaaaagtgg
    13801 atcttgtaaa attctcttcc aaacaatatt gtcaaaaccg tccacaaagc ctgccttaag
    13861 gagactcttg gtgtgaatga ttccaattac attgtcctta tccccatcat aaaccgggat
    13921 acgagaataa ttttgtttta aaatactttg gataatggct tgactatcat cctgaatatc
    13981 caccataaag gcatccgttc gaggaaccat aacctctcgt gccatcagtt catcgagcga
    14041 aaagacacct tgtagcatct caatctcatc agcatccaat gtttcttcac tatttgtcag
    14101 catataggca atttcatcac gggtcatctt ttcatccgca tcatcgaatg acataggagt
    14161 caaatggctc aagaaattgg tcgaagcagc taaaagccaa acaaaaggac tgactagttt
    14221 tccgatccca atgataaccg gcgctgtacg aattgccaag gcatccttta gattaagagc
    14281 gattctctta ggatataatt ccccaaaaac gatggaaata taggtcaaaa atgccaagga
    14341 tagaaaagtt gccacggctt gtgctgtttc gccattccca agccaagagg caatcacacg
    14401 tcctagagta tcagttaaac tcgcccctga taagattgta atcagggtga ttcctacctg
    14461 aatggttgat aaaaagtggt taggattttc tagtaccttc agcaggcgaa tgtagcgtct
    14521 gtctccttct tccgcctttt gttcaactcg ggcacgatta agagaaacca tggccatttc
    14581 tgtggctgag aaaaaggcat ttaataccgt caagataaac aataaaacaa attgtagcaa
    14641 caaattctga ctacttgggt cttccatagt tcttctccta aaatagtatc ttatccttta
    14701 ttatatcaca aaatttaatc caatacatat tatttttttc ttagcgtaac ttccatctct
    14761 ctcccaaaac tggaagttag tctaagacgg atttccactg atttcacgcg tttttctcat
    14821 actaaaagct aggttgtggg aaactggaag ttagttttag aagttactat tttttcttag
    14881 tacctgttct agccctgctt aaactagtaa aaagaactcc tacatccata agtgacatag
    14941 gagtttaggc ttatctttta ctgagttaca gttacttctc cagttcggta atccagttga
    15001 agtccctttt gaacattggg aactagaaca ttgaattcca attctccatc cgaagacttg
    15061 gcttcaatct gtgaagctga gggaactaaa tcctcgtttg aagcgtagta aagggttaca
    15121 cggtaacgga cacgcttgtt ttggtccaag gctttacgca ccttgctttc atagtagttt
    15181 tgaccagtcg aatactcggc ttgtgcctga tttgcccagg ctgtctgaac agcaatgttt
    15241 ttaggattgc ttgttgaggc atcaaaacca tccaaaccac cgattaaggc atagcctaac
    15301 aaatgacctc tatcgactgc atgggtataa gagcccttta gattcttgac ctgatgccaa
    15361 cctggaggag tccaagaagt tgaaccattc ccagtttctt tacgattctt gtactgacga
    15421 gtggccttag acaagagggc attagctacg gttggaacag tttccttgcc cactgtcttt
    15481 gttttattgt cagcgtaggg cttacttgaa accttggcat ctagatttgt tttattacca
    15541 ttgacgataa aagcacctga gccattccac tccagactcc cctttatttg actcttgact
    15601 gcgtctgtta agacactctc tgccaatgct tgactaggag cttcaggcgc ttgttttttc
    15661 tgactaagct tggttttagg actattaggt gccgactgca tctgcttgat ataatagctc
    15721 cctgtagaca aaagcaataa cactagcagt ccgattagtg tctgtcttgt ttttttgttc
    15781 atatttctcc ttatctttag aaaaaggctg gttctccagc ctaatttcct gtaaatttat
    15841 gaatcaattc ctgccatttt gctggagaca ggatagccca tggatcttga cccttgccca
    15901 agattccata acctaccatt aaaccgattc ctagagccag agtacctaaa atcagtacta
    15961 tgatgactaa aagtaaacgc ttgactacgt agcttgattt cttattcatc ttcatcactt
    16021 gcctcaatcc gctgaatctt agcacctagc tgcgccaact tctcatggaa accgtagtaa
    16081 cctctatcca agtgaaccaa tttaccgacc acagtttctc cctgtgctac caaacctgtc
    16141 aaaatcaagg ctgcactggc acgaaggtca gttgaaagaa cttctgctcc ctgcaaaggc
    16201 tgtccaccaa caatacgagc tgtatcacgg ataatctcag aatgcaagcc catgcggcgc
    16261 atctcttcta ggtgttggaa acgattttcg aaaactgtct ccaccatggt tgattcgcct
    16321 tttgcaactg tcatcaaggc tgtaaattga gcctgcatat ctgttggaaa tcctgggtgg
    16381 ggcaaggttt tcacatgaac agcttttaga ttttctagtt gagaacgaac acgaattcct
    16441 tcgtcttctt caattacttc aacacccatt tcaagtaact tggcaatcaa gggacggttg
    16501 tgctcccaga cagcgtctcg aatcaagaca tcaccaccag tcatggcagc agctaccata
    16561 aaggttcctg cttcgatacg gtcttggact acattgtgag tcgtaccatg aagtttctca
    16621 acaccagtaa tggttatagt ctctgtacca gcacctttga ccttggctcc catttcatta
    16681 aggagaatgg ctaagtcaac aatctcaggc tcacgcgcag cattctcaat cactgtcacc
    16741 ccatcagcca gagtcgctgc catcatcaag ttctgcgttg caccaacact tggaaagtcc
    16801 atatagatat gagcgccatg caagcgttct gccttggctt cgatgtaacc agctgtctga
    16861 ctaatcttaa cccccatagc ttccagacct ttcaaatgaa gatcaatagg acggctacca
    16921 atcgtacaac cacctggcat ggataccttg gcatgaccca cacgggcaag gattggccct
    16981 aatacaacga tggaggcgcg catcttgctg acatacttgt aaggggcttc ctcagtgatg
    17041 tcgccagtag catccacctt gacaagatga gcttcctcat caaagtcaac cttggcattc
    17101 aaaccaccaa ctacctgatt cataataaag acatccgaca aaatcggaac attctgcaag
    17161 acggtctttc cttcacttgc tagaatagtc gctgccaaca agggtaagac tgcatttttt
    17221 gctccctcga tcgtcacgct tcctaccaga cgattatcgc caccttgaac cacaatttta
    17281 tccatactcg tttcctttac tcttgatttc ataataatcc tctaagcgct tcttgccaca
    17341 aatgatacag gctaataaag aaagaactca ggatgtagcc tatgacaatg ctaaaaaagg
    17401 ctactaataa acgaactttt cttgtattat catctgtcac ttttaaaacc ttctcccatc
    17461 taacaagatc ctttaaaagg taaaaactca cataaataaa gaacatatga ctacttagag
    17521 tgaataataa ttgaaccatt tgactattat accatcttct ctgtttgtgc gctagtttgg
    17581 aaacttcact caaaaattag ggattgtttt tcaagtaatc aatcgcatct tgtagggttt
    17641 taacgggcac gattttcata tccgtcttga ttgttttagc cgcttctagg gctgtttggt
    17701 agttgttttt cgcgtccgga tgcgcctttt gttcttcttc gctaacaggg ttatcaggag
    17761 caaagaaaat agcagcacct gccctagccg aagctacaac cttcttatca atacctccaa
    17821 tgtctcccac attaccatcg cggtcaatgg tacctgtacc ggcaacaata cgaccattac
    17881 gaagatctgg gtgagctatt tgagtataga tagctagact aaacatgaga ccagcacttg
    17941 gaccgccaat accagctgtt gaaaagctaa ttgggacatt gctgattacc tctgtacggt
    18001 caatcaagcc gattccaatt ccatttttgc cattttccaa ggtgatgatt tttccttctg
    18061 cagacttggt ttgcccatcc tcttcatagg tgaccttgac ggaatcccct aatttttgag
    18121 aactgacgta atcaatcaag tctttggaac tatcaaaggt ctgatcattg actgctgtga
    18181 ctgtatcaga gatattgaga atccctttaa aggttgaatt atccgtcaca ttcaaaacat
    18241 aaactccaaa gtacttgagt tcgatatcct taccagctgt ttttagtcct tgatacttgg
    18301 ccatattttg cgatgtttgc atgtagaatt gattgattcg cataaattca acatcggaag
    18361 aaccacctgt agtctcctga gcactacgaa tatctgtaaa aggtgtcaac caagcataaa
    18421 tcatatgagc taaagtggca tgttgaacac caaccgtaac gaattgatag gcaccagctt
    18481 ccttatcttc tgtgtcattt actttaagga cttggcgaat atcttccgaa ccacctggaa
    18541 cctctatata ataaggcaag ggcactacaa atgccaagaa agtcacaatc aaggccgcaa
    18601 tgacgtataa gggccatcta atcttttttt tcatttctta tttcctccaa aatactctcg
    18661 ggaacatagc aggcaatatc ctgaccaaac ttcaaaagct ctctaacgcc tgatgaactg
    18721 atatagagat gttcaggtcg actatgtaaa taaatagtct ctatatcaga agacagctga
    18781 tgattgtagt aatcaaaact ggcttcatat tgcaaatccg acgcatttct caagcctcgc
    18841 actaggcaag tagcccccag tctttttgcg acatcgacca ccaatttatc atgagaagac
    18901 acgactttaa catttcccaa atgtttcaca gccttttcta acccccgttt acgattttca
    18961 agagggagaa atccttgttt gtggggatta aaaaaaatac ccacataaag cttatcaaag
    19021 agtctgctcg cccgttcaat catatccaga tgcccatttg tcatcggatc aaatgagcct
    19081 gtgaataagc caatcttatc tgacatagac tgtcacctta ctaattccat aaatcttttc
    19141 cttccagata cccagacagg caatctcttc tggaagttca acggccttat ccgtctcgca
    19201 cacaaccata acatcttcag aaaaaagctc tctctcagcc attttttcaa tatctgctac
    19261 gatttgttcc ttggcatagg gagggtctaa gaaaacgagg tcaaattccc cagatacctg
    19321 ttccaatgcc ctctctgcat ccatcttgag gagttgaaat tttccaactt ccttggtcat
    19381 ctggatattt tcagccacga tggtctgagc cttacggtct cgctccacca aaacagcact
    19441 ggacatgcca cgcgatactg cttcgataga taaaccacca ctacctgcat aaaggtccaa
    19501 gactcgtccc ccttcaaagt agggaccaat catgttaaaa atggctcccc taaccttatc
    19561 cgaagtaggt cttgtcgtct tgccttctag tgtcttgagg ggacgtcccc catagattcc
    19621 tgatacgatt ttcatactgt ttattatacc aaattataga caaaaagaga aagaaaaccg
    19681 aaccttgcgg ttcgattctc tacaaaatat tttcgtaagt atcgcggact tcttgaggcc
    19741 aaacacttgt ttgcacttct ccgatgtgtc tcttgcgaag taggaacatg gccatacgag
    19801 attgtccaat tcctccaccg attgtcaatg ggaatagacc attcaacaaa gacttgtgcc
    19861 attccaattc caagcggtct tcatcacctg taatttccac ctgacgtcta agagtttctt
    19921 catctacacg aattcccata gaagacaact caaaggctcc acctaaagac tcattccaga
    19981 caagaatatc accatttaga cccttgtagc cattctcaga ctcgcttgtc cagtcatcat
    20041 agtctggtgc acgtccatcg tgcggtttac catctggcaa ctcgccaccg ataccaatca
    20101 aaaagacggc tccaaattct ttacaaatcg cattttcacg ttctttcggt gtcaagtctg
    20161 ggtagcgttc taccaattct tctgtatgga taaaggtgat ttgttttggc aagatagact
    20221 cgatgtcata gcgggcttca acagctagct cagtcaggcg aatagcctta taaatcttct
    20281 caactgtttc ttttagataa acgatgttac gcttaccatt tgggataacc ttctcccagt
    20341 cccactggtc aacataaaca gagtgggttg catccaagga atcctcatct ggacgaaggg
    20401 ctttcatgtg gacaaagaga ccctctcctt caccaaagcc aaaacgagcc aaggtgtggc
    20461 gtttccattt agcaagtgag tgcaccactt cataagtagc atcagggatt tggagaacct
    20521 tgaccgatac tggattttcc acaccagaca ggttgtcctg cattccgtca ccgaccttac
    20581 tcaagatagg accttgaact tcgacaactt ctagcttatc tttcaaatac tgggtaaaag
    20641 tgtttttgac aaaggaaatt tcttcttgtt gatggataaa acttttcttc atagacaact
    20701 cctcaaaaat ttaataaacg tattatacac ctatttccaa gaaaagaaaa gagaaaattt
    20761 aaaaaaatca gtgtcactca aaacactgat ttcatagacc ttttagtcat tgcgaccaaa
    20821 gatacgtaga atacttagga aaagattgat aaaatcaaga tagatgctga gagccattga
    20881 caccacccag cctgtcgcta cacgaccttg tgattgttca taggcgaggc gaatcttttg
    20941 gttatcccaa gcaatcaacc ctgaaaaaac caaaaccatg gctacgctaa tcatataatc
    21001 aaagaagcca ctagccaaga aaatattgac taccatagca atcagtaaac caatgagagc
    21061 tgccatcatt gctcgaccaa tcccactcaa gtctttcttg gtaaagatac caactgccgc
    21121 catgacaaag aagagaaggg cgctcgatac aaaggcagat aaaactgtac ccggagtata
    21181 gaaagccacc acaaaactaa gggtaaagcc gtttaaaacg gagtaaagta aaaatactgg
    21241 aagagcagct ggactatttc ttgaagccat gctactggca acgaaaacca aagccaactc
    21301 cgcaaaagta gcaatggtca accagagacg cccctgcatc aacaagtaaa ccaactgaga
    21361 ctgaaagacc gtcaacataa ggcctgatac caaagcagat agtccgattc ccagaccaac
    21421 aaaggcataa accttagcgt aaaattgatt gagacctgca cggtcatgta taatagtgtg
    21481 attcatcctt ttctcctttt ctgtgaacat ctttccttga ttataacaaa aaaagttaaa
    21541 attagcttaa atggaagatt aaaataccgg ttgcaactgc aacattcaaa ctctcggcct
    21601 gccccttcat gctaatatgg accaactggt ctgcactttc agccataagg ggactaattc
    21661 cttgaccctc atttcccata actagtacaa aattttctat agaaggcagt tctctgtaat
    21721 caacagaatc tttagatagg gttgttgcta gcactgggat agctaccttt ttagtttcgt
    21781 caagaagcgc ttgactagtc atccggtaaa tgggcagatg gaaatgacta ccttgcatgg
    21841 aacgtagggt tttgagactg tagatgtctg ccgacttatc tgaaacaatc actcctgtaa
    21901 aacctgctgc atccgcagtt cgaatgatag ttcctacatt accaggatct tgcacatctt
    21961 ccaaaaacaa gaacttgccc tgactcaaat cagcttgtcc tacttcttct ttttgaacca
    22021 cggcaacaat tccctgtgga gtctgagaat ccgccaaatc tagcaaaata tcctctgaaa
    22081 tccagacagt ttgcggaaaa gcagctaact gatttcgata attttctagg gcaaagattt
    22141 tctcaatcgt cactccagct tggacagctt cttcaaacaa gtgccagcct tcaatcaaat
    22201 aggcagactt gcggtatttt ttttggtgta atttcttggc attttttacc acagaattgg
    22261 cttttgaggt tataatagtc atagaaatat tataacacaa tcaaaggggt ttggtatgca
    22321 aaaggttaga atgattgccc aaggtagggt gcagggagtc ggctttcgtt ggggtgttta
    22381 tagcctggcc cttgaaatcg gtggcatcac aggtcgggta tggaataacg acgatggcac
    22441 agtggaaatc ttagcccaag cagactcatc tgctatcatg gcaaaattta tccaagaaat
    22501 ccgaaaagga ccgacacctt tttcaaaagt aagctactta gatgtcaaac taagcaactt
    22561 tcctccctac tctgacttta aaatcgcaaa ttaggtctcc agaactattg tatattttgt
    22621 aaaaaaacag tagaatagaa aggtataatt tttaaagaag gaataaaaac agtgaaatct
    22681 attaaacgtt ttgcattctc ggctatggga gtggctatgt tgcttgtctt gactggctgt
    22741 gtcaatgtcg ataaaaccac aggtcagcca acaggattta tttggaatac gatcggagcg
    22801 cctatggctg aagccatcaa gtacttcgct actgataaag gtctaggctt tggtgtcgct
    22861 atcattatcg taaccattat cgtacgcttg attatcttac cacttggtat ctaccaatca
    22921 tggaaggcaa cgcttcactc tgaaaagatg aacgctctca agcatgtcct tgagccacac
    22981 caaacacgtc tcaaagaagc aactactcaa gaagaaaaac tcgaagccca acaagccctc
    23041 tttgccgctc aaaaagagca cggcatcagc atgtttggtg gtgtaggatg tttccctatc
    23101 ctccttcaaa tgcctttctt ctctgctatc tactttgctg cccaacatac tgaaggggtt
    23161 gctcaagcaa gctacctagg cattcctcta ggttctccaa gtatgatttt ggttgcctgt
    23221 gctggtgtcc tttactatct tcaatcgctc ctttcacttc acggagtaga agatgaaatg
    23281 caaagagaac aaatcaagaa aatgatttac atgagcccac tcatgatcgt cgtcttctcc
    23341 ctcttctcac cagctagtgt cacactttac tgggttgtcg gtggtttcat gatgattctc
    23401 caacagttta tcgtcaacta tatcgttcgt ccaaaacttc gcaaaaaagt ccgtgaagaa
    23461 ctagccaaga acccaccaaa agcaagtgct ttctctaaac caagtggacg aaaagacgtt
    23521 acccctgaac aaccaactgc tatcacaagc aagaaaaaac acaaaaatcg caacgctgga
    23581 aaacaacgtt cgagataaga agaaaaaggc ttagctaatt tgctaagtct ttttgcaaat
    23641 caaaagcccg atgtttccat caggcttctt ttctatccat gtacacgttt catataatct
    23701 tgataacttt cggtatccat gagtttttta gcgttcttga cgcgatctgc tgttggtggt
    23761 ttgactcctt cgagggaata tggaattcca agttcacgcc acttgaactc acccatggtg
    23821 tgataaggta gaatttcaaa cttatcaaca tttttgaggg tcttgacgaa cttaccaagt
    23881 tcaatcaagt catcatctct gtctgtcaat cctggaacta gcacgtggcg aatccagaca
    23941 ggttttccaa tatctgatag atactgggca caagccaaga tatttttatt agtttggcta
    24001 gtgacaatct tgtgctgttc ttcgttgatt tccttgatat ccaaaagaac caagtcagtg
    24061 acagccatga gtttgtcaaa cttctcaagg taacgtggtt tattacggaa aggaagagca
    24121 caggtatcca aggtacagtg gattccttgt tccttagcct tggtgaagag agcaatcagg
    24181 aaatcaatct gcaagagagc ttctcctcca ctgactgtaa tcccaccctt atttccccag
    24241 aaaccacggt agcgcaaggc ctctgtcaag acatcatcta ctgtccgttc acgtgactta
    24301 ttggactcca tagcccaagt gtctgggttg tggcaatact ggcaacgcat gtgacagccc
    24361 tgcaaaaaga caataaagcg aataccaggc ccatctactg acccaaagct ttctgtcgaa
    24421 tgcaccattc ctgtcacttg tccataatca attgtttctt cagacatatc gttaccttcc
    24481 ttgaaaacgt tttatagttt tattatatca cgacttgaag gaaaaacaag atttttgcct
    24541 attttttaga aagcaatctg tttttcaatt tcattttcaa tctattatca gattattctt
    24601 caaaatcgaa gccatataag gttgcaaaat agtcctcagg gcgctctgca cggcggattt
    24661 gacgggcttt accttcttcg gtatagagga tttccgcaga acgtaatttg gcattatact
    24721 ggtagcccat tgaaaatccg tgggcacctg tatcatgaat taccagcaaa tcaccgattt
    24781 ctgtatgagg cagttcgcga ttcactgcaa atttatcatt gttttcacag agtgaaccaa
    24841 ccacatctac cgcttcagct ggtccatctg gatgggtcac gttgctaata tgatggtaag
    24901 ctccgtacat agctggacgc atgaggttga ctgctgaggc atccacacct agataggtac
    24961 ggtaggtttc cttcttatga gtcactcttg tgactagagc accgtgaggt gccagcataa
    25021 aacgacccaa ttcggtgaaa atcttgacct gaccaagacc tgctgacgta agaacttctt
    25081 catacacctt acgaactccc tcaccaatca aggcgatatc gttcggctcc tggtctggac
    25141 gataattaac accaataccg ccagaaagat tgataaagtc tagcgaaatg cccaactttt
    25201 ccttgatttc aacagccagt tcaaagagct gacgagccaa ctctggataa tagagatggg
    25261 tcacggtatt ggacgctagg aaggagtgaa tcccaaaagt cttggctcct ttttccttca
    25321 agatagcaaa ggcttcaaag agctggtcct tggtcatgcc aaacttagcc tccccaggat
    25381 tgtccataat gtctgtcccc agttcaaaaa cgcctccagg attataacga caagagatga
    25441 tttctggaat gcctgctact ctctccagat gttcaatatc ttcaaaggca tccaagttaa
    25501 tggtcgcacc caattcacgt gcataggcgt attccttgtc tggcgtgttg ttggaagaga
    25561 acataatctc agaacccaga aagtccagtt tatggctcat caaaagctct acataactag
    25621 agcagtccac accacaacct tcttcttgga gaattttcaa aatagctgga gttggagtag
    25681 ccttaactgc aaaatattcc ttaaagccct tgttccacga aaaagcttgg ttgacggctc
    25741 ttgccttctc acgaatcccc ttctcatcat acaagtgaaa gggagtcggg aactcggcaa
    25801 caatcgcttc taactcttct ctattgataa atggtgtttt cataagcttc cttctattct
    25861 gtttgcaaag aaaggctggg acaatcgttc caacctttag ttctatctct tatttatctt
    25921 cgtcaaagat attgccaacc tcaacatcga tggcttggtt cttgacatca atattggtta
    25981 ccttcaagag tgacttgtag tcaaactgct tttcacgttc ttcttgggca ttgtccgcaa
    26041 agaccgctac acctgccagt tctgagtcga actcgcgcaa gagactaatc ataccattga
    26101 ccgttccgcc acctttcaag aagtcatcca caatcaagac acggctgcct gccttaagac
    26161 tacgttttga aaggaacatt ttctcgatac ggtcaccact tgaacctgaa acatagttga
    26221 cgctaacagt tgaaccttcg gtaattttca ggtcacggcg cacaatgaca aaagagacat
    26281 tgaggacatt ggcaactgca tttgcaagtg gcacaccctt agttgctacg gtcataaccg
    26341 cgtcaatttt ttggtccata aagcttttgg caataatacg accaatattt ttcaagatgg
    26401 ctggtgtgct aagcaaatca gacagataga tataaccacc tggcaagata cggtcacttt
    26461 ctgacaactt ggtacgcaag tcttcaacca tttccttagc atcctgactc gaaatagacg
    26521 gtgtgaagat gacacctccg ccagccccag tcactgtctg gatatgaccg atttcaattt
    26581 cctcaaaggc gcgtttgata atgacgatat cttctgagat ggatgattta gcagactcat
    26641 acttttcagc aaaagtattg agactagtta gtttataagg attattaatc aaatagttgg
    26701 aaatgacaac catccgatca cttcttctta atttcatttc tatttcctat tccattttta
    26761 ttcaaaaaat caaaaagcaa actagaaagc tggtcgctgg tggttcaaaa cactgttttg
    26821 agattgtcaa tagaactgac aaaccctgta atatacctgc atatatacat acgacaaggc
    26881 gatactatcc tagtttgaag agattttcga agagtattca tttttgtctt ttacttatta
    26941 taccatattc acataaaaaa acgaacattc ttatcctaaa aaatgctcat ttttcttaaa
    27001 ttatcaatct aaatctggtt tatagaagga acgattatcc atagcgaaga ttttattggt
    27061 catctctcct ttatccacca aagccagagc tgttgacatc atcatcatgc ttgcatccag
    27121 attgtcaatc atatggataa tctctgcctc cataatacgt ggacggactg gacttccata
    27181 ttcaagcaag ccgtggtgac tgaggatgac atgacgaagc aaaacgactt cttccttggt
    27241 atcatcgatg ccgagttcca taactgtctt ggtaatttcg ctatcaatga gagcgatatg
    27301 tccaagaaga ttacctcgca ctgtgtactc tgtctggtct ggccccgtca actcgataac
    27361 cttagctaag tcatgcagca taatccccgc atagagcagg ctcttattga gctgaggata
    27421 aacttcgcta atagcgtctg ccaaacgcac catggtcgcc gtatgatagg ccaagcccgt
    27481 ttcaaaggca tggtggttgg tcttggcagc tggataggag tagaattcct tatcatactt
    27541 ggtgtagaga tttcggacaa tccgttgcca gacaggattt tcaattttga aaatcatttg
    27601 cgacatgtag tcacgaattt ccttgacatc aactggtgac ttgaccttga aatcagctgg
    27661 gtcattgggt tcaccagctt gaggcaggcg gagagtaatt tgattgactt gaggggtatt
    27721 gttataaact tctcggcgtc ctttcatgtg gacaacctta cctgcggtaa aggcctcaat
    27781 gttatgaggt tgggcatccc agagcttccc atcaatctcg ccactatcat cttggaaggt
    27841 aaaggctagg tagtttttcc cagctcgagt ttgcctcagg tcagctgatt tgattaggta
    27901 aaagccttca aataactcat cttttttcat gtgactaatc ttcatattct tcctcatttt
    27961 cttgaaaatg gagtagatca agcgcaggct caccttctga caactcaatg tgacggagcg
    28021 tccgctcgat agctatggta cgacggttta ataattcatc aatattgcca gaggcatgtt
    28081 ggagatgttt ttgtgccttg accagaatac caccaaactt gccaaactcg gtcttgacac
    28141 tggcaagagt cttgctgata tggtcggcac tcttttggat attaagggtc ttgaaaccaa
    28201 ctgataggga gttaagaagg gctgataggg tacttggtcc tgcaacgata atctgttctt
    28261 cccgtctcaa atcatcaaag aagaccggat tgcgcacaat ctctgaataa agaccttctg
    28321 tcggaacaaa caagattcca aaattggttg tccgaggcgg tgctatgtac ttgctcttga
    28381 tatccttggc aaagcgcttg acgcttgcta ggagtgactt acgacagcgt tcaatctcat
    28441 ccttgtcacc tgtctcatag gcttcttcca agcggtaata atctgccagt ggaaacttag
    28501 agtcaattgg cagatagacg tattcttggt cgccttgtcc gggtaacttg atggcatact
    28561 ccactcgttc actagagttt tcaaccgttg cgtattctcg ttcgtactgg gcaggtgtca
    28621 tgatgtcttc aataatttgc cccagttgca attctcccag aatacctcgc gtcttggttc
    28681 cagagagaac cttgttaaga gctccgacat cacgggcaac tgtctgcatt tctccaaggc
    28741 cacgattgac agactccagt tgtttagaaa ctgtttcaaa ggaagcctgt aagcgtgtct
    28801 gcaaggtctt ttctagtttt tcctcgaccg tctggcgcat ttgttccaaa cgttgctcat
    28861 ttgattcctg caaggcttgg agacgttggt ctgtcttgtc tctagtttgg aggagattat
    28921 ctgtcatttc ttgacggact tgggtcaggc cttggtgcaa ttcaatccgc acttcttgca
    28981 aacggtcgct gacagccact tccaaatctt tttggtctaa ctggctggct tgtctggctt
    29041 ggtcaaagcg gtaatccaac tggtctgaca aatgatctgc ctgatcctcc aagctcttac
    29101 ttaagtgttt ctcctgccta tcctgccttt gccaaatcag aaagagacca gctaggttgg
    29161 caattaataa tagaataagt aaactttcca tcctacctcc tgtccttgct atgcagtacg
    29221 accacataac catctgggca agttaccgac acttccctat ctatatattc gttagaagcg
    29281 tacacttttt taaagaaaaa attctcctct gtcaactcat acttagctcc aagaatagtc
    29341 agctggctat cccgaactgg cataaaggct agatagtcgt agtctgaacg aggttctagc
    29401 tgactggtcc cttctgaaca ataggcaatc aaattttgcc catcctcaat ttctatttga
    29461 cgcatatagg gtgccaactt aggattgcta ggtagaaaga cattggccaa catatggtca
    29521 atacggccac ccaaggcacc gaaaatagtg acctgagcct gaggattttg ttcaaagatg
    29581 gttaagagag ccaattccag atctgtatca tccttttctg gtcgggcttg gacaaaatac
    29641 tgggcacgtt tttgaatcac ctgtcgctct tcttccgtca cagaatcaaa atctccgact
    29701 gctagagcaa gaggtaagtc ttcttccaag acccagagcg agcctcgatc caccccaaca
    29761 aaagcatcaa aatctgtccg ataatgaccg cggtttccgc ctgcaaaaac tgcaacccta
    29821 gtccagttgt tttctgagag tttgtactcg ctcattgact tctcccttaa agacatagga
    29881 acctgctaca aaaacagtcg caccagcttc tttggcttga gcaatagttt ggtcatcaat
    29941 cccaccatcc acttcgattt caaagttcaa acctttttcc tcacgaagag caaccaactc
    30001 acgaacctta tccatggttt ctggcagaaa ggcttgccca ccaaaacctg gattaaccgt
    30061 catgactaaa acttggtcaa ctagatgaag gacgtgcttg atggcttcaa ctggtgtgcc
    30121 aggattgata acgactgaag gcttaactcc gagtgaacga attttttgga gggcgccatg
    30181 aatatgaggt gttgcttcta catggatact gatgatgtct gcacctgcac gcgcaaaatc
    30241 ttccagatga tgctcagggt ttgacaccat caagtggcaa tcgaaaacca tcttactatg
    30301 aggacgaagg ctctcgacca cacctgcacc aaaactgatt tgcggtacaa aatgactgtc
    30361 catgatatcg atatgggcat attctgcccc agttgcttct agacgtttga tttcacgttc
    30421 aaagttggca taatctgctg ccagaattga cggagcaatc ttgtattgag acataggttt
    30481 ctccttattt tggaattttt ttgctgactt ttttataggt ttctctacga ttttcaattt
    30541 cactaaggaa ttgcaggtaa ttgtcaaaac ggaaggttgc aataacaccc tcttcaacag
    30601 ctggtttgac ggcacaagac ggctcatggg tatgggtaca agtacggaac ttacaatctc
    30661 ggctaacagt agcaatctct gggaaagcct gattgaggtc ttcagccctt gatacttcat
    30721 agtccaagga tgaaaatcct ggtgtatctg cgattttacc cccgttgaga ttgtaaaaac
    30781 taacagctcg agtggtatgg cgaccgcgac ctagactgtc tgaaatttct cccgtttcaa
    30841 gattgaggtc tggtgcgatt ttattgagaa gagttgactt cccaacacct gtctgcccca
    30901 taaagaccgt aaccttgcct gttaacaaag acaggagttc ctctttactg gtcacaaagt
    30961 catagccgat gtcaccatag gtctgctggt aaaaatccag ttctccccta tcttccaaca
    31021 aatccatttt ggaaatatag acaatgggat ggatgccctt gtgctccaaa agaaccaaga
    31081 aacgatccag caaattgctg ttaaaatcag gttccttgac ggacatgatt actacagctt
    31141 gatcgatatt gacaataggc ggacgaacca gactgttttt ccgttcgtga attttgagga
    31201 tatagccttc tgaattttcc tcggcagaga aatctaccca gtccccaaca taaggggtat
    31261 ggcctttttt acggaaattc ccacgcgcgc gtgtttgata aacctggcca tcactctcca
    31321 catagtagaa acctgccaag gctttaatga tttgtccctg catcttagag tccttgccct
    31381 ttaagtgcgt ctgctagacc ggcaaattct gccaagctga gagcttcccc acgcacactt
    31441 ggtgacaagc ctgcctggtc caaagccttg gtcagcttgt ccttgacctc ttcagtctta
    31501 ccaaagtaac ctgtcaagtt attccacaag gtcttgcggc gatgggtaaa actagccttg
    31561 gaaaccttaa agaaaaagtt ctcatcttct actgctacgg ctggctctgg acgacgcacc
    31621 attttcaaga tggctgaatc cacatttggc gctggcacaa agaccgtacg aggcacgata
    31681 aaggcaacct tggctgtcat gtaatactgc acggcgatag acaagctacc gtaagccttg
    31741 gtgttaggct gggctgaaat gcggtccgct acttctttct gcatcatgac cacaaactca
    31801 caaaaaggaa tgccactctc aatcaagtgc atgagaatag gcgtcgtgat gtagtaaggc
    31861 aaattagcca ctaccttgat tggcaggtta ggatttttaa aattttggat atgttgcgcc
    31921 aaatcaacct tgagaatatc ttcgttaact acagtcacat tatcaaaatc acgcagggta
    31981 tctaccaaaa ttggcaccaa acggtggtca atctcaaaag ccatgacttg ggctgcacgc
    32041 tcagccagaa actccgtcaa ggcaccaata cctggcccga tttcgatgac attgacctgg
    32101 tcatcaattt cagccgtatc cacaattttt tgaaggatat tggtatccgt caggaaattt
    32161 tgcccgaagg actttttaaa ggtaaaaccg tgacgctcca gcactgcctt ggtcactcta
    32221 taatctgcaa ttctcatcta ttctcttttc tattatctgt tactactatt gtaacacatt
    32281 ccccttgtat tttgggaatg aaaatcatac caaaaaacta tctttcctca tgaagaaaga
    32341 tagtcttgga aatctttaaa agtggcctta acagttctct cgaaacatgc gttcaaaaaa
    32401 atcagcttct tgataacttc gattttcagg tagggtcaaa tccacatcct cctccacatc
    32461 atccactgtc agttcaaccg tctgaggtaa atttttcaaa cgataatgct tttgcctttg
    32521 cccttcaata ctagcaaaat caagcaaata gccatgcact cggatttgaa ccaaaaggag
    32581 atgattggtc atattttcca tgatgaggat atcttctaaa ccacctctgg cactaggata
    32641 atcagatacc ctgcgaatac ggattttttc acacatgagc aagtttcctc atatcaatga
    32701 tttcatcagc cttattccag actaggggat tatgggtcgc aatgatgata attcgatttt
    32761 catctttcaa atccaccaag agattcataa cctcctctga attttcagga tcaagagctg
    32821 ctgttggttc atctgccaaa atcaagggtg gatttttcaa aatagtctta gcaagggcaa
    32881 ctcgttgggc ctctccccca gataaagtat aaattttttg ttctaaatcc aaatacccta
    32941 gattaacttt ttctaaagcc cccacttgcc tttccaaacg ttctactttt gagattttct
    33001 gaccaacaaa acccaaatcc aaattttctt tgattgattg gttttctaag aggccaaaat
    33061 tttgaaagag atagcccatc tgatctcgaa aatactcacg agtgggaatg gtttttaaat
    33121 ctttcccctg atagagaacc cttcctccat ctatcttttc tagctttccc aagatattca
    33181 gcagcgtcgt ctttccgctt ccactctttc cgattaaggc ataaaccttg cccttctcca
    33241 gcttgagatt caaaccagag aaaatcgcgc ggtcattaaa tttcttttcc aatccttgaa
    33301 tatcaatcat cttattttcc tttcaatact gccatgctac cagcttcttc ttttttgtcc
    33361 tgccttacta aaatcaagag ggcgttaagc gtaaacaaag ctacaactag agcgctaatc
    33421 aaaccatctc ttgtcaaaat actagatagg actaggccaa gcaagagaac tcctccttgc
    33481 gccagtaaat acttgccatg aagctcatag attgtcatac cagcaagacg tttaatcata
    33541 agctcccgtc tgaactgctc aaaatagaga agattcatgg aatcaaataa caagatagcc
    33601 gtagacaggg tcaaaatcgt cccaataagg agagagtaaa actccacccg tttgtcattt
    33661 agtaccttgg caaaaaatag ctggcttttt accaagtaag aaaccttgtg atacagacct
    33721 tgctctttta gagctgtgat gctgtctcca tatcgatcta gtttcaaggc actattagcc
    33781 gtagttcccc acaacatccc tgcaacaggt cttgttccag tcgcttgcgg cgttagaact
    33841 acaataatcg gatcttttag gtactggcgt gttgtcttgt acccatcatt atagaggaaa
    33901 cgttcctgtc ctgtttctgt aaaagctagc cttacctgtg ggaagtagtg tttctgactc
    33961 gtcacttcta cactttcaga tgaaaagttt tgcaggtaat ctgtaaacaa tccttggtag
    34021 taggcagact gctctcgcaa gctctcaggc aagatcagcc caaactctcc ctcagacaag
    34081 ttttgcatct tgtcaataaa ctctgaagaa acggtaatct tttcttctat cagatagcgc
    34141 ggcgagacat agataacatt ccctgacggt atgtagtcac tgagacgatt gccatctagg
    34201 tccacttctg ctccatctga gaaattgtca acattgctca taatataaaa agagtctgta
    34261 ttggctaacc gttcttcagt aaatgtctgc cactcacgat tatccttacg cgttccttct
    34321 tcatcggcaa atgcactaga ccaaccaaag gatagacggt aacggtctga ggactggctc
    34381 catttattgc tagctctctc catttcctgc atttcacggt agtggggtag gagagctgtc
    34441 gcactcgatc cgaccaccaa tacagctaag agttgcccca ccatcatcaa tgtcatcatc
    34501 cgtttgagag ggagtttccc tttcaataga tccaccagac tattttcctg taaaccaagt
    34561 agatggacga cacttagtaa ggtagaaatc cctgccaggg tcaatccata aagtagaaga
    34621 gcaatgatga ccagttgtac cgttgccata aacaaggcac cttgatacca gagaatcccc
    34681 aatcccaaaa gactggatac cagcactgag cagataagct gtctcacatc ttctaacact
    34741 ggtctgagag caactccaaa caagctctca ccagctatta agcgaatccc tgcctgacga
    34801 agggatttga tccgataaat cagggtcaga ctcataaagg tcagcagaaa aatagctaaa
    34861 ctcagtagca ccataggggt ggccgccaat agtaagacta tcgaaaatgg atcttctcca
    34921 ttcgaaacaa agccttgata gccaagttct ttcaaggtgg tctgtaagct cactccatcc
    34981 aaacttcctg acacaatcaa gtaagaccct aataaatcac tcgtttctgc actctccttg
    35041 gaggaaatgg tcagaccttc tggaagcttt ccctcaccat aagtggcata ggtaaagcgt
    35101 gtttctccag cttcatttgg ctcaacgatt cgacgggcga tgagactatt atgctctacc
    35161 gccaagtgac tcaaagcctc tgtcacctct tcgcgactag ctactggttg atttatcatc
    35221 attcctgtca catcaacaga aggatagtta ccaaataaaa tctcttctcg atggtcaacg
    35281 gaagttatca ccatataaag cgataccaat accattgaaa tcaaaataaa taaacgtttc
    35341 atcatttcct ccaaaaagtt acttggtcac ttagtgagaa ggtgaccaag taaagctaaa
    35401 caatagggct ataccattct aaacattata gtagaatgca actttctctc cccaagaagt
    35461 acgaatccat gcacgagaag tccctccagc accagcatat cccttgtcag atgaaccagt
    35521 ttttctgcta acaactgttg aactatggta ttttgaaggg tgatagtagt ttgagtaagc
    35581 accaccgtta cctgaaccat aagtccaaac accaccttct gggtattgaa cggctgcaga
    35641 ggcaactgct acacttccac ctaatgttgc tgcaatgctc gctacaagag ctaatttttt
    35701 aactgtttgt ttcatattga tactcctcta tttttgatta caagattgtt gagttacttg
    35761 ctcctctttc cattatcaac gttgatacta ttatattacc aaatgatatt ctaaaattat
    35821 ttgtttttct ctaaattgtc aaaatagcac cctgaaatga acttttatag tcattatact
    35881 ccatctttat cacactatca aggacatcaa tgtccttaaa atctaactat ttcatattat
    35941 ttttagctta tatctaaaaa aacagttcta aagacgaact ttagaactgt tttgatacga
    36001 ggacaaggac atttttgtca ctgattcact atgtttataa atattttttt aaatcttctt
    36061 gccattgttt ttctaacata gtaactaaat aagaattttc tattatatca gcaaatagct
    36121 tcgatttctg atagaactgt tcggcagaaa cataatcatt gtttattatt agagataatt
    36181 tccactccaa cattaatata attggtttct tctggaaatc ttgtgttttt tccattatat
    36241 gattaaggct aaagatataa gctcctagtg aatcataaga cgacaagtta tccaaacagg
    36301 ctagacccga aaacaaagtg ttacttaaag caaacagata ttctgatgca attaattcta
    36361 catgatttgt caaacgaact aaaaacgaat gaattctgtt aatttcttca atctgatttt
    36421 gaccttgttt tatctttgta agcactatag caaaatataa gttgataact tctaattcat
    36481 ttatcaaaaa caattcctta tcataaattg ttttaaaata atcgtccagt acggtttccc
    36541 cgtattctgg tcgtccactt cgaataacat ctatcgttgc tctagtagct tcaaaccata
    36601 ctttttcctc attaggaagt tgctcataaa ataaatcctc tatctcttcc aaacacgctt
    36661 ccttcttatc gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca
    36721 attctaaata agcagaagga agagctgaaa aatccggcat aaggctataa actggaactc
    36781 ctagccggcg agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa
    36841 ttcgcgacag ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa
    36901 ttcctttttc tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctat
    36961 tctacctgct tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta
    37021 ctcataagat ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg
    37081 tttgaggagt tgcttgccgt tggaatagcc gattcggaga gtctctccga ggtattctct
    37141 acgcttacgg ctgtctgccc ctgctagaaa accaaggcga atcaaatcgc tacgactaat
    37201 gtcaaactgg ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc
    37261 ttcatagctg gcatgctcaa ttcccagaga acgccccttg gtcttggact tgggaacagc
    37321 ttcatctcgc ttgagaaagg catgctgaac tgttggaatg accgtcatga tcatgcgccg
    37381 aatccgttct ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag
    37441 gcgctgaatc cgctctatat cctgatcatt gatggcagaa cctcgagtct cataggtctc
    37501 cacatcgaaa taacgcttga gattgaccgt atcatcacgc ccttcaacca cgataacttg
    37561 agaaattttc tctttcatta cttgctgtcc agtccaaaaa ttcgttctgc atttgcagtc
    37621 gttgctaccg ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca
    37681 tagcgagtat aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc
    37741 gcatctgttt ccaccaaaat cttgtccaaa agtaactctt tagctgcttc ttggaggtca
    37801 gttgccttct taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac
    37861 ttctctgccc actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg
    37921 ccctcactct tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa
    37981 ggcaaatcca agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct
    38041 ttgggcgctg tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga
    38101 tgttttaact tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta
    38161 ggatgccaac caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc
    38221 tcaatcgtcg gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc
    38281 aaggcaattt cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta
    38341 tcaaaaatca tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg
    38401 gcacctaatt tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc
    38461 tatccgatat tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga
    38521 tagaatattc ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca
    38581 tcactaggat tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag
    38641 aattttctag ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact
    38701 tgttgccacc actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg
    38761 taattgtttg ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg
    38821 cggtgcactt gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac
    38881 catggcaatg gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc
    38941 attttcaatc tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc
    39001 tttttcttta tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt
    39061 ttcaatggca tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg
    39121 agctgagttt tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgccttcatt
    39181 cgcattagca tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag
    39241 cttatactct ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt
    39301 atcttttcct agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc
    39361 tccagctact tcaactttat agtaaaataa aataataaca ggacaaatcg atcaggacag
    39421 tcaaatcgat ttctaacaat gttttagaaa cagaggtgta ctattctagt ttcaatctac
    39481 tataacatcg cttccgaatc catttgcatc tactgaactt ttttacaaaa ataaaaatca
    39541 gtagactcga gttcctactg atttttgata gaattcgttt ggattatgca gccaaaactt
    39601 tgcgtccttt acgacgacga gctgccaata cgcgacgacc atttttagtt gacatacggt
    39661 tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg ttgataagta cgtttcacga
    39721 tgaatacctc ctcatagatt ttgtattcgt ttagccggct ataaagtgat cagttactaa
    39781 acatacttta ctattctatc tgattctttc acttttgtca atagccctag gcaaatgttt
    39841 ccagcttttt cgaatgcaag ccctatctac gatactgttt caagaaacga gtcatctttt
    39901 cttggatttg ggctaactca tcaacacgag gaaggtaaac gatacggaag tggtctggtt
    39961 cctgccagtt aaagcctcga ccatgaacca agagaacctt ttcctgcttc aagaaatcaa
    40021 ggacaaactg ctcatcatca tcgatacggt acatattgcg gtcgattttt gggaagatat
    40081 agagccccgc cttgggttta acggcagaca aacctggaat atcttgaatg gcattataga
    40141 tgaaatttct ttgctcgtag attcgtccac caggaagaag caattcatcg actgattggt
    40201 gaccccccaa ggaagtttgt acgacttgtt gggccaaaac gttagagcaa aggcgcatat
    40261 tggacagcat attgagccct tcgatatagc ccttaacatg agtcttaggg ccagacaaga
    40321 ccatccaacc cacacggaaa cctgctatgc ggtgggattt tgacagacca ttcatgctga
    40381 cacagaagac atctggtgcc aagctcgcca caggcgtatg cacatgtccg tccattacca
    40441 tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt ttgacgggca atctcaataa
    40501 tctccagcaa gagtttctta ggataaaggg ctccagttgg gttatttgga ttgataagga
    40561 cgattgcctt ggtattggaa gtgatttttg acttgatatc gtcaatatct gggtaccatt
    40621 caacagcttc atcacagata tagtgaacgg catttcctcc agctaggctg acagccgccg
    40681 tccatagagg atagtctggc attggcacta agacctcatc cccattgtcc aaaagcccct
    40741 gcatggacat gacaatcaac tcactgacac catttccaag gtaaatatca tcaatatcta
    40801 cgtttgggaa tttcttcagc tggcaatact gcatgatggc cttacgggct gagaaaattc
    40861 ctttggagtc agaatagcct tcactatcac gcgcattcat aatcaagtca tgaatgacct
    40921 cgtctggcgc tgtaaagcca aattctgctg gatttcctgt attcagacgt aaaatctttt
    40981 ctccattcgc tcgcatccgc atggcttctt ccaatacagg accacggata tcataagcaa
    41041 catgctctaa cttactagac ttgttatatt ctttcatctt gtttcctcaa ttcatcagga
    41101 tagtaacatt ataccactag aaagagaatg cgacaagttt actgaaatgt gttactagtt
    41161 tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa cgtagagata gcaggtattt
    41221 tgcaaagaca catcacatac caaaagataa acctagaaaa agcaagccta gaaaggagtc
    41281 ataaagaaga ttaaacatga ggaaaagccc ccatatcatc aagtaatccc aacgcccact
    41341 tcgttttgca actagaaata agattagatg gtagagtaca tgaaaaacta aaaatccaat
    41401 aaaacttgga taaagaagcg gtaccagact ctctaattga cagatcatga tcacttctac
    41461 caagactgaa actaggataa ttccataata aaggaaattt gatttttgaa aagagttttt
    41521 catcattctc ccttccttca cttctttcct gctatccttt cttttatttt atcatatatt
    41581 tatatagagt ttaagcccta atattctttt gaaaaatact ttttttgctt tacttctata
    41641 gtgaaaaggt ttacaattat agtaagaaaa tttcaggagg tttcattatg gcacaacgtt
    41701 accaaaatat catggtcgca atcgatggtt ctaaggaagc ggacttggct tttgtcaagg
    41761 gagttcattc tgctctacga aacgacgcta aactcaccat cgcacatgtc attgacacac
    41821 gcgctctcca aagcgtatcc acctttgatg ctgaagttta cgaagaactc caagtcgacg
    41881 ctgaaagtct gatgaaagag tacgaaaaac gtgctaaaga tgctggggta gcagatgttc
    41941 atatcgtcat tgaaatggga aatccaaaga ccctgctagc acgtactatt ccagatgccg
    42001 aggaagtgga cctcatcctc gttggcgcaa ctggtctcaa cgcctttgaa cgcctcttgg
    42061 tcggctcttc atctgaatac atactccgcc atgctaaggt cgatttgctg gttgtgagag
    42121 aacaagaaaa aaccttataa tcacaaagaa aaggagcccc tagctccttt ttgtttacga
    42181 tttatttctc tctttatggc gttcgtaagc cttgagctgg cgctgcagtt cctttttaat
    42241 agcaggttct ggagcatatt tttcttccca attatctggt tttaagattt tatgggtcac
    42301 tggatcaaaa tgagccttgc catctggaaa aattttcccc atattggcct gatggacaat
    42361 atcaaaaata cgttctgggt ccacccccat caagacaaaa ctgccgtagg tgaagtaaag
    42421 cgtgtcaatc aaggcatcca cttgccctat caaatcttgc tgagcaggtg tcttcttggc
    42481 tactttatct gctgccttat caagggcctg atgaagttgc gatacagctt gaccaaaatc
    42541 ttcttcagaa ggactggctg ctcgaacaaa ctccaccaat tcttctattt taaaaccagc
    42601 cctatgggtt gcaccctcta aatcccaagc tcgaggttct tcttgggttc gttcatccat
    42661 catgtggtgg aaggtcttga ccttattgaa atgatagtca cggctgacaa agactttttc
    42721 tgaagacaaa acaccaaaat gttccaaggc cttgtggatc ccatcttgtt gattgctagt
    42781 ggtaatgtgc ttggcagctt ccttgacact gctactacca tttcccatag cgaccgacat
    42841 accgacgcca gccagcattt caaggtcatt atctgagtca ccaaaagcca tgacttgatt
    42901 gaggtcaaag ccatattctt tcccaactcg gcgaatgcct tctaatttag attttccctg
    42961 attgatgaca tccgatgcaa aaggattgct acgtgtcaat ttcaagtctt caaaatcagc
    43021 tgccgccttc tcagattctt ctggtgtcat cagcatcaaa acttggtaga taggctgatt
    43081 catcaggtga agcaggtcct cttccttttg gggaacaacc ttgctgacca tgcgattaaa
    43141 agactgactc accgtccgag ttaaaacaga gggaacgaag cgactaattc gttgggaaaa
    43201 agaacccaga ccaaaggaca tgattttaga acccaacatg gcatccttgg tccctagggc
    43261 aatctccgtg ccctcttttt tagcatagct aattagatgg cgcaaatgta acttggaaat
    43321 agggctcgtg aacaagactc tgtctttact aaagatatac tggccattat aggttaccgc
    43381 aaaatccaga tccaaatcgt ccatcaattc cttaacaaaa aaaggtcctc gccctgtcgc
    43441 tacgccaact agtacccctt gttctttgac aatcttaatc gcatccttag tggatttcaa
    43501 aacactcttg cgattgttga ccaaggttcc atcgatatca aaaaaaacag ctttgacttc
    43561 catcctatcc caatctcccc ttttgtgata caatgattat accacatttc agaaagagtt
    43621 agtaaatcat gcctaagaaa atccttgttt tacatacggg tggaactatt tccatgcagg
    43681 ccgatgcttc tggcgctgtt gtgacgagtt cagataatcc catgaaccat gtgtccaacc
    43741 cacttgaagg aatccaagtc cacgccttgg acttttttaa ccttccaagt ccccatatca
    43801 aacccaaaca tatgctggtc ctctaccaga aaattaaaga ggaagcagat aactacgatg
    43861 gagtggtgat cacacacgga accgatactt tagaggaaac agcctatttc cttgatacca
    43921 tggaagttcc ccatatgcct atcgttctaa caggagccat gcgtagctcc aatgagctcg
    43981 gtagtgatgg tgtttataat tacctaagtg ctttacgagt ggccagtgat gacagggctg
    44041 ctgacaaagg agttttggtc gttatgaacg atgaaatcca cgctgccaag tatgtcacca
    44101 aaacacatac gactaatgtc aacaccttcc agactccaac acatggcccc ctcggtctca
    44161 tcatgaaaca ggaaatcctc tacttcaaaa cagctgaacc tcgtgttcgc tttgaccttg
    44221 atcacataca aggtttagtc cctatcatct cggcttatgc tggtatgaca gatgagctga
    44281 ttgatatgct ggatttagaa cacttggacg gtttgattat ccaagccttc ggagctggta
    44341 atattcccaa agaaacggct caaaaattag aaagccttct gcaaaaagga atcccagtcg
    44401 ctctggtatc acgatgcttt aacggtattg ccgagcctgt ttatgcctac cagggtgggg
    44461 gcgtacagtt gcaaaaagca ggcgttttct ttgttaaaga actcaacgcc caaaaagccc
    44521 gcttgaaact cctcatcgcc ctcaatgccg gactaacagg acaggctttg aaagactata
    44581 tggaaggcta atactcttcg aaaatctctg caaaccacgt cacgtcgcct taccgtatgt
    44641 atggttactg acttcgtcag ttctatctac aacctcaaaa acatgttttg agctgacttc
    44701 gtcagttcta tctacaacct caaagcagtg ctttgagctg acttcgtcag ttctatctgc
    44761 aacctcaaaa cagtgtgttg cacagccttt aaccagccgc ctagtccgct ctatggtatt
    44821 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
    44881 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
    44941 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatggc
    45001 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
    45061 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ccttatcagc
    45121 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
    45181 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
    45241 taccaaaccc tcactataag cgattcctgc ttttttcaag gtttccttgt agccaactaa
    45301 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
    45361 ttctttagca aggtaactca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
    45421 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
    45481 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
    45541 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
    45601 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
    45661 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
    45721 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
    45781 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
    45841 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
    45901 ttttcctgtc ctttctatct cacacattct tttacaagta gaggtactga ttgaagctct
    45961 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
    46021 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
    46081 agaaaaccta ggtttagctc cttgctacca ccttagacta aacaaaaagg aggaaactaa
    46141 gccctcctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
    46201 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttcc atctactata
    46261 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcatgaaa atattatatc
    46321 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
    46381 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
    46441 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
    46501 ctgtcaacgg attgggacgg gtcatcacca cttccatcaa ttcaggcgaa tactccttgc
    46561 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
    46621 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
    46681 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
    46741 ctgtcttaac aggcatttct acgtcaagga tggtgatatc tacagactcc ttttctaata
    46801 gttggattgc ttcttgccca tttttggctt gaaggacaga ctccacatct ggttgaagcg
    46861 tgagcaattg acacatggca tctcgcaaca tactttgatc ttctgcgact aatactttca
    46921 tcttagtttc tctccttata aggtagtcga acttgcactt ccgttggatg tttctgactg
    46981 attacactta cttctcctga aaatggaaaa acacgttctc ggactgtatg gagctcatct
    47041 ccttttagaa aagcaaagcc gcagccatca tcacttactg ttaaaatgag ttccttctct
    47101 gtccgttcta attttaagta agctttagac gctttggcat gtttgatgat attggtcacc
    47161 aactcaagca aaatcataga agccattgac tccaattctt gagtaaggct agcagtatct
    47221 agttggttat ctgtttccac ctcaattcca gcaatttcta acatcttttt cacagtctct
    47281 agttcggatg tcaaagttct agacttaaga ttttccacaa tggttcgaac ttcacgcatt
    47341 gattctttgc taatttgctg tatttctctt aattcctttt ccacttgtgg ataagcctgc
    47401 atttgaaata gctgcaaggc tagatctgtc ttgacactaa gcatagcaaa ggtatgtccc
    47461 agactatcat gcaaatcctg accgatacga ttgcgctcat tttctgctag caatagattt
    47521 atctgggtat tttgcttgac ctgagcttct ttcaaatcct cgacaaggcg aattcgaacc
    47581 gagcccaaag tcatcaaatc gacaaaagta agaattacaa gcatatagac tagaaactca
    47641 acttcgattc tctgaaaaat caaaagtcgc cccacaacaa ggacttgagc aaggagaaat
    47701 gtccatacat gtaaagactt taaactacgt acgccaaaat gataaattaa gagattggaa
    47761 aggaaaaata aaaaccagat ataattccca gcaacaaaga cggtattccc agctacatag
    47821 accagcatga tcccccagaa aagccaagat aggcgctggt tcttagttat taaaacaccc
    47881 aaatacgtca ctacaaatag aatatcaacc aataaatgcc aagcaggaag ctccccaatc
    47941 actacaggta ggatgggaaa aagcataaaa atcaaactgg cacaaaacat atagtgtatg
    48001 cttttcagtc tttcaagcat taagcattct ccttatgacc ttgaaggtaa atagtcaaac
    48061 caaataaaac tgctgaaaaa acaagtaaat aaactgtggc tgatagattg atgccaccct
    48121 cgtttaagaa ggtcttgagc aactccatca actgataagt tgggagacac ttaccgatcg
    48181 cttgcatcca gtctggaaat aaagagatag gcatccagag tccgcctaaa acagccaatc
    48241 ctagataaag aagattgccc acaacagaca tcagctgact agttggtaag agagtcaagg
    48301 tcaagccaag ggctacaaaa gctacacttc ccaaaataag caaaatcgca gccccaaccc
    48361 agcttcctag aggaatctct acacctctta caaagtgccc aactgagaaa accaccaaga
    48421 ttgaaaccaa ataatcaacc agcatacttg ttatctttga tagataatat tctaccatat
    48481 ttacagggct atgacgtaat gttttctgcc agttgttgat tttatctgta tgtaaaacag
    48541 ctgggaatga aaacatagct gtcgacatca tggaaaaggc tgtcatggag atgaggtaat
    48601 cacgcataaa attagctggt ccacctggtg tctcctggta gatgccagaa aagaataaat
    48661 agaaagccgt cggcattcct acggataata aataatagac gagttgccgt ttggtcaata
    48721 aaaattctat cttatttaat gctatccatc gtttcatctt agtcatctcc tttttgcgtt
    48781 tcttcaaaga ttgtatccag caaactacga ttattaactt cgatttcttg catcctacat
    48841 cctgcttggg ctaatagttc ccagaaagca tctgcttctc gagtgactac ttgtaaagaa
    48901 tcttgcttta gggtccagtt ttcaaccaaa tttgactgct ctacgacttc cttgtaagct
    48961 ataggaagga taaagtgctt ttctatttcc tcgctacgca tagctagagg cgtcgtatct
    49021 cgaatcaact ctcccttatt taagagcaaa atgcggtctg cagtgtgttc cacttcctca
    49081 atataatggg acgaatagag aatggtgact ccttgcgctt ttaactcctg gacaatttcc
    49141 caaaaacgtt gacgagttga ggtatccatg gacgcagtag gctcatctaa aaaaacaatc
    49201 tttggtcgcc caattaaggt caagacgaaa gaaaagagac gcttttgacc gcctgataat
    49261 ttttctgcca actgttcttt ttgttgcttg acaaactgca atagttgatc aatttcctga
    49321 ttgctcaaag gatttggata gatacgttga aagaaagcaa tcaactcttt aacttttaat
    49381 ttctgaacga ttacattttc ttgagggagg taagccctag tatagtctaa cttagaactc
    49441 gtcactggta aaccttgaat agatacttga ccgcttgtga ccagtttatc tccaagcaaa
    49501 caatctaaga gtgttgtctt tccggcacca tttggaccaa ttaaggcaac acattcacca
    49561 tcgttgattt caaaggagat attccgcaaa atttccttgt cttttatttt cttactcaat
    49621 ttctcaactt taaccaccgt catgagactc tcctttcaac cactccattc ccataaggaa
    49681 aacgacgaaa atcataaatc caaaccccaa agcaccacga atgaattggc gaagcaaggc
    49741 ttgatcaaac caatcagtaa acatttccac taaccatacc aagagtgaca ggccgataaa
    49801 gaaatagatg atccctctct tcattcctca aactcctttt tcacatctct gactaatttc
    49861 aaaccttctc taacaagcca agacatgatt ccaaagccag caaagacctt ccaaggaaaa
    49921 tggtagaaat cctcatccaa tcccgaaaac atgagatagg tcataactcc tgctactact
    49981 aaacttactg cgataatcat tttatttctc atctcttctt cccccatttc atactacaat
    50041 tatagtcttt taaaatcaga ggagacagaa gcttctgtca ctagaaaata tgacaaatgt
    50101 cataaaaaat tctgttcaaa acaagcaaga tacactatac aataaaacac aatcagaaaa
    50161 atctaaggca acttcctcaa aagagatatc aaacccaatt cacaccataa tgtaaactaa
    50221 tacttattta aaatcaaaaa gagtaaaaat ttttatcaga caaacacat
//
LOCUS       DBERCA010000010        46333 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.010, whole genome shotgun sequence.
ACCESSION   DBERCA010000010 DBERCA010000000
VERSION     DBERCA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 46333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.010"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..777)
                     /locus_tag="ACL719_000649"
                     /pseudo
     CDS             complement(1..777)
                     /locus_tag="ACL719_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1073..1378
                     /locus_tag="ACL719_000650"
                     /pseudo
     CDS             1073..1378
                     /locus_tag="ACL719_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            1510..2454
                     /gene="manA"
                     /locus_tag="ACL719_000651"
     CDS             1510..2454
                     /gene="manA"
                     /locus_tag="ACL719_000651"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGS0698663.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGIFILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(2475..3953)
                     /locus_tag="ACL719_000652"
     CDS             complement(2475..3953)
                     /locus_tag="ACL719_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HGS0698664.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSAIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFEKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSLWSSLPNLCNQKGLE"
     gene            4058..4807
                     /locus_tag="ACL719_000653"
     CDS             4058..4807
                     /locus_tag="ACL719_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGS0698665.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLYTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(4827..5282)
                     /locus_tag="ACL719_000654"
     CDS             complement(4827..5282)
                     /locus_tag="ACL719_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS0698666.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5302..6476)
                     /locus_tag="ACL719_000655"
                     /pseudo
     CDS             complement(5302..6476)
                     /locus_tag="ACL719_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(6535..7380)
                     /locus_tag="ACL719_000656"
     CDS             complement(6535..7380)
                     /locus_tag="ACL719_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0698667.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSIQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            7505..8062
                     /locus_tag="ACL719_000657"
     CDS             7505..8062
                     /locus_tag="ACL719_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS0698668.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8081..8548
                     /locus_tag="ACL719_000658"
     CDS             8081..8548
                     /locus_tag="ACL719_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGS0698669.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8637..9266
                     /gene="upp"
                     /locus_tag="ACL719_000659"
     CDS             8637..9266
                     /gene="upp"
                     /locus_tag="ACL719_000659"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGS0698670.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9440..10030
                     /gene="clpP"
                     /locus_tag="ACL719_000660"
     CDS             9440..10030
                     /gene="clpP"
                     /locus_tag="ACL719_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGS0698671.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10109..10357
                     /locus_tag="ACL719_000661"
     CDS             10109..10357
                     /locus_tag="ACL719_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGS0698672.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10459..11619
                     /locus_tag="ACL719_000662"
     CDS             10459..11619
                     /locus_tag="ACL719_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0698673.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINGAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11887..12756
                     /locus_tag="ACL719_000663"
     CDS             11887..12756
                     /locus_tag="ACL719_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS0698674.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12760..13716
                     /locus_tag="ACL719_000664"
     CDS             12760..13716
                     /locus_tag="ACL719_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS0698675.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13716..14480
                     /locus_tag="ACL719_000665"
     CDS             13716..14480
                     /locus_tag="ACL719_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698676.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            14480..15190
                     /locus_tag="ACL719_000666"
     CDS             14480..15190
                     /locus_tag="ACL719_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698677.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            15498..16154
                     /locus_tag="ACL719_000667"
     CDS             15498..16154
                     /locus_tag="ACL719_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGS0698678.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16261..17356
                     /gene="prfB"
                     /locus_tag="ACL719_000668"
     CDS             join(16261..16332,16334..17356)
                     /gene="prfB"
                     /locus_tag="ACL719_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGS0698679.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            17374..18066
                     /gene="ftsE"
                     /locus_tag="ACL719_000669"
     CDS             17374..18066
                     /gene="ftsE"
                     /locus_tag="ACL719_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGS0698680.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            18059..18985
                     /gene="ftsX"
                     /locus_tag="ACL719_000670"
     CDS             18059..18985
                     /gene="ftsX"
                     /locus_tag="ACL719_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGS0698681.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSAVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            19253..21433
                     /locus_tag="ACL719_000671"
     CDS             19253..21433
                     /locus_tag="ACL719_000671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGS0698682.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            21485..22300
                     /locus_tag="ACL719_000672"
     CDS             21485..22300
                     /locus_tag="ACL719_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS0698683.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            22440..23783
                     /locus_tag="ACL719_000673"
     CDS             22440..23783
                     /locus_tag="ACL719_000673"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0698684.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLEIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23998..25188
                     /gene="metK"
                     /locus_tag="ACL719_000674"
     CDS             23998..25188
                     /gene="metK"
                     /locus_tag="ACL719_000674"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGS0698685.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            25623..26558
                     /locus_tag="ACL719_000675"
     CDS             25623..26558
                     /locus_tag="ACL719_000675"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGS0698686.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIKELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            26592..27629
                     /gene="holA"
                     /locus_tag="ACL719_000676"
     CDS             26592..27629
                     /gene="holA"
                     /locus_tag="ACL719_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS0698687.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKVDSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27802..28407
                     /gene="sodA"
                     /locus_tag="ACL719_000677"
     CDS             27802..28407
                     /gene="sodA"
                     /locus_tag="ACL719_000677"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGS0698688.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            28564..29094
                     /locus_tag="ACL719_000678"
     CDS             28564..29094
                     /locus_tag="ACL719_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGS0698689.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISCLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SHKEDKGQRHFVIRQK"
     gene            29115..30200
                     /gene="rlmN"
                     /locus_tag="ACL719_000679"
     CDS             29115..30200
                     /gene="rlmN"
                     /locus_tag="ACL719_000679"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGS0698690.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVDCNIGCTFCASDLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITVSTSGLAHKIRNFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEYGT
                     DIDAACGQLRSNTMKRDRQKAVAEVNP"
     gene            30202..30675
                     /locus_tag="ACL719_000680"
     CDS             30202..30675
                     /locus_tag="ACL719_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS0698691.1"
                     /translation="MTKKRELILRLGVAVYSLCIVYFCFTPQATVEVGTPGIQHLGRL
                     VFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKKVLL
                     FSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN"
     gene            30677..32218
                     /locus_tag="ACL719_000681"
     CDS             30677..32218
                     /locus_tag="ACL719_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0698692.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMTEDGADVDALMEEVGELQDRLESRDFYILDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKSLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(32274..33077)
                     /locus_tag="ACL719_000682"
     CDS             complement(32274..33077)
                     /locus_tag="ACL719_000682"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGS0698693.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNHALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(33160..33372)
                     /locus_tag="ACL719_000683"
     CDS             complement(33160..33372)
                     /locus_tag="ACL719_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698694.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(33852..34061)
                     /locus_tag="ACL719_000684"
     CDS             complement(33852..34061)
                     /locus_tag="ACL719_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698695.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
     gene            34231..34503
                     /gene="rpsP"
                     /locus_tag="ACL719_000685"
     CDS             34231..34503
                     /gene="rpsP"
                     /locus_tag="ACL719_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGS0698696.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            34523..34762
                     /gene="kphA"
                     /locus_tag="ACL719_000686"
     CDS             34523..34762
                     /gene="kphA"
                     /locus_tag="ACL719_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGS0698697.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            34911..35235
                     /locus_tag="ACL719_000687"
                     /pseudo
     CDS             34911..35235
                     /locus_tag="ACL719_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            35339..36127
                     /locus_tag="ACL719_000688"
     CDS             35339..36127
                     /locus_tag="ACL719_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0698698.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            36158..36676
                     /gene="rimM"
                     /locus_tag="ACL719_000689"
     CDS             36158..36676
                     /gene="rimM"
                     /locus_tag="ACL719_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGS0698699.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            36666..37385
                     /gene="trmD"
                     /locus_tag="ACL719_000690"
     CDS             36666..37385
                     /gene="trmD"
                     /locus_tag="ACL719_000690"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGS0698700.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            37397..37735
                     /locus_tag="ACL719_000691"
     CDS             37397..37735
                     /locus_tag="ACL719_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGS0698701.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(37765..38085)
                     /locus_tag="ACL719_000692"
     CDS             complement(37765..38085)
                     /locus_tag="ACL719_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698702.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            38183..38398
                     /locus_tag="ACL719_000693"
     CDS             38183..38398
                     /locus_tag="ACL719_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGS0698703.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(38395..38583)
                     /locus_tag="ACL719_000694"
     CDS             complement(38395..38583)
                     /locus_tag="ACL719_000694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698704.1"
                     /translation="MTQYPFFYFENLYSMKIKEQTRKLAAGCSKHCFEVVDETDEVSN
                     HTYGKAKLMRFEEIFEEY"
     gene            complement(38603..39139)
                     /locus_tag="ACL719_000695"
     CDS             complement(38603..39139)
                     /locus_tag="ACL719_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGS0698705.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            complement(39335..40681)
                     /gene="gor"
                     /locus_tag="ACL719_000696"
     CDS             complement(39335..40681)
                     /gene="gor"
                     /locus_tag="ACL719_000696"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGS0698706.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            40967..42166
                     /locus_tag="ACL719_000697"
     CDS             40967..42166
                     /locus_tag="ACL719_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGS0698707.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVANEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            42150..42851
                     /locus_tag="ACL719_000698"
     CDS             42150..42851
                     /locus_tag="ACL719_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698708.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            42853..44112
                     /locus_tag="ACL719_000699"
     CDS             42853..44112
                     /locus_tag="ACL719_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698709.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            44236..46233
                     /gene="metG"
                     /locus_tag="ACL719_000700"
     CDS             44236..46233
                     /gene="metG"
                     /locus_tag="ACL719_000700"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGS0698710.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFCLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT        14171 a         8596 c        10059 g        13507 t
ORIGIN      
        1 atctttaagg tacttgattt atgccatata gcattgtcca taacgagtaa aagataatca
       61 tctggataag cttgtgaaag ctcttctaaa aaggcgttca tccactcagt attacatcca
      121 ccagctatta agaaaaatga ttcgcctgta tgggcatcaa cagctccata acaatagtga
      181 aattctcgta tatagtgact atggacatgt ggacctactc ctattggagc ccaacaagat
      241 cccagtttac tgattctacc gaaaccagcc tcatcttggt acatcaagcg aaccttacga
      301 aaacgtctac tgcttttaaa gcgctttctt ttcttcttga attgagattt tatttttaga
      361 cgcgacaatg gtttgagcgt ctgctttcct aggatgttct ggacgtggca taatatttcg
      421 ccaaccatgg tgcttcaaca gttgatagaa ggcatcacgt gtgtaggaac gacctaactc
      481 ctttttataa gcctgaaata aggcatcaat tgtaacaaat tctcctgcct ctgtagcctt
      541 caaatggcgg gcaagaaaga ctttcttttc ctcaatcgtc atatatgcat ggttacggcc
      601 accacgtgtt tcttgaagga gagagtcgag ttcgaactcc tcatattttt ttacatttcg
      661 ccaaatcgtt gtttgattac agtctatagt gtattgaatc tataacagta caccttagct
      721 gctaaaatat ttctataaat tagtttgact ttcctgatag agttgttcac atcttatttc
      781 aaactactat aaaagttcta taatctcttt ataagatttg cccatcagac gaaatataat
      841 aaattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat cgatttgact
      901 gtcctgatcg atttgccctg ttcttgtttc gttttactat agaacgattt gaaggcgttt
      961 atgatattta gctgtacgag agtctttaaa aagtgttttg atggtttgga tttcttcttt
     1021 agttgatttc atatcactat tatataatgc tttttgattt tagtatagta ttatcgtaga
     1081 aaatggactc ggtgctatgg atacaccgga tgagaatggt tatgtagcag atgactatcg
     1141 gattacttac ttagaggccc acatcaaggc catgcgagat gccatttaca aagacggggt
     1201 tgacttgctt ggttatacga catggggctg tatcgattcg gtttcagctg gcacagggga
     1261 aatgaataag cgctatggct ttatctatgt agatcgagat aatgtaggaa atgggactct
     1321 caaacgtagc aagaagaagt ccttctattg gtacatgtca tttatagcaa tggtgtaagc
     1381 atcgagtaga gcacatttgt ttactatttt tataaaatct tctccctgct ttataagatg
     1441 taaaaaagag ttcaattcga actctttttt tgctataatg aggggagaaa aatcagacag
     1501 gagatcgaca tgtcagaacc attattttta caatcagtta tgcaagaaaa aatctggggt
     1561 ggagccaagc tacgtgatga gtttggctac gacatcccaa gtgaaaaaat cggagaatat
     1621 tgggccatct cagcccatcc aaatggagtc tctaaagttg ccaatggtcg ttacgaggga
     1681 acagatcttg ctactttgta tgcggaacac cgtgaattat ttggcaatcg tccagaacct
     1741 gtatttccac ttttgaccaa gatcctcgat gccaacgact ggctcagtgt ccaagttcac
     1801 ccagacgatg cttatggact cgagcatgaa ggcgaactcg gaaaaacaga atgctggtac
     1861 attatcgcag cggatgaagg ttcagagatt atctacggtc acaatgccaa gtcaaaagaa
     1921 gaactccgcc agcaaatcga ggacaagaac tgggatgact tgttgaccaa agtccctgtt
     1981 aaggctggag atttcttcta tgtaccaagc gggactatgc acgctatcgg ggcgggtatc
     2041 ttcatccttg aaacccagca gtctagcgat accacctatc gcgtctatga ctttgaccgc
     2101 aaggatgaca aaggcaactt gcgtgaactt catcttgaaa aatccatcga tgtcttgaac
     2161 attggtgagc cagcaaatag ccgtcctgta actgttaaag cagatgattt gcgttccact
     2221 ctccttgtat ctaatgattt cttcgcagtt tacaagtggg aaattactgg aaaagttgac
     2281 tttgaaaaga cagctgacta cagcttattg agtgtcttag ctggtcaagg tcagctaact
     2341 gttgacggga aaaattatcc aattcaaaaa ggcagccact ttatcctacc aagtgatgtt
     2401 gaagcttgga ctctggaagg gcaaggtttg gaattgattg ttagccatcc ataaaaagaa
     2461 agggcctgag ttcactactc aagtcctttt tgattacata aattgggcaa tgaagaccac
     2521 aatgatgatg attggaatga cggaacgaag aaacgaagaa ggaagagcca gacttggaac
     2581 agtccttgtt tccatgctct ttcatcgaga tggagttcct ccattgcaag agccttttta
     2641 aagatatagc ctgtaaaaag tgaaaggtag agagctccaa atggcatgag gagattggaa
     2701 accaagaagt ccatagcgtc aaagaaggtc ttaccaaaaa tgtgaacatc cgccatgaca
     2761 ccgtaagata gggctgaagg aatgccaaag acaaaggtca aaattcctaa aataacactc
     2821 catttggcac gtttgctgtt atcctggttg gtgatattgc ccacattgat ctccagcatc
     2881 acgacagaag aagtgacagt cgcaaagagg aagaggacgt agaaaatggt tccaaaaggc
     2941 atcttgtcaa agagttgagg caagacgata aagagcaggc tgggtccccc ttcagactgg
     3001 atattgaagg gggatcgagc ttggaaaatg gctagacctg ccatgatgga tatcgagata
     3061 ttcatggcta cgatggagat tcctgactgg actagattgg ttttcttgtc caagtaagaa
     3121 gcataggtca acatgactgt aacccctagt gagagggcaa agaaagattg tcccagagca
     3181 tagaggagac cagcgctagt cagttttgaa aagtctggtt tgaggaagta aagaacccct
     3241 tccatggcat ttggcaaact gagagagcga ccgatgataa caacaaagat gataaagagc
     3301 aggggcatca tgactttcga agctctttca atcccttttt gaaccccacg tgatacaatg
     3361 aagatattca ataggataaa ggccgcttga gctcctaggg caatggctgg atttgaaatg
     3421 attgaagtaa ataactgagc ataatcaccc gttccaccaa gttggaacaa tttcccaaac
     3481 tcaataccta gatagactag aatccatcct ccgataacac tgtaaaaaga taagaggata
     3541 aagagggcaa aggcgccaat ccaaccgata aagttgtact tgttattctt gcccagtttt
     3601 tcaaaggttt tgatagcgga aacgccagca ctacggccaa gggcaaactc agccagcagg
     3661 agagggaaac cgattaaaat agtggaaatg agaaagatta gtaaaaagcc tccaccgcca
     3721 ttagcagcag tcatgtaggg aaacttccaa acggaaccaa gcccgatggc cgagccagca
     3781 gatgctagaa taaaaccaag tttcgacccc cattgtgatt tttctgacat agttaaactc
     3841 tgatctcctt tttcaaaata agaaaaagct acttgagttg tcaaatagcc ttctgtttga
     3901 ttgatagact tgactatcct atcatgtttt ctaaggtctg tcaagaaaat cattttcaag
     3961 ttttcatacc tttctcaaaa aagttaaaaa attttctcaa aaacgcttga ctctgaccta
     4021 aggggagggg ttatactatc attgtaagga ggaaatcatg taccatataa aagaagctgc
     4081 gcagctttca ggtgtctctg tcaagaccct gcatcactat gacaagatag gactcttggt
     4141 ccccttaaag tcggaaaacg gctatcgaac ctacagtcaa gaggatttgg aacgccttca
     4201 ggtcattctt tactacaaat atctaggctt ttctttagag aaaatagcag agctgttaaa
     4261 ggaagaaagg acagatttat tgccccattt gactaggcag ttggactatc taactcgcga
     4321 aaggcaacat ctgtatacct tgatttccac cttgcaaaaa actattcaag aacaaaaagg
     4381 agaaagaaaa atgaccattg aggaaaaatt cacgggattt agctatcaag acaatcaaaa
     4441 ataccaccaa gaagcggtag agaaatatgg tcaagaagtc atgggacaag cgctcgaacg
     4501 ccaaaaaggt cacgaagacg aggctacggc cgcctttaac caagtctttc aaactttggc
     4561 acaaaatctt caagttggtt tacctgcaac agcaaccgaa aaccaagagc aagcagccaa
     4621 gctcttgcaa gccattcgca cttatggatt tgactgctct attgaggtat tcggtcatat
     4681 cggtaaaggt tacgtctaca acccagagtt taaggaaaac attgacaagt ttggttctgg
     4741 aacagcccag tacacgtcag atgccattgc agcttacgtt cagacaaatg cagaaaaaat
     4801 aggctaggaa tttcctagcc tattttttac ttcaaatcat aaagccagtc gtcaccgttt
     4861 ttgtagtaaa agaattcact gagatcttct tctagaaaca cacgaagcat atcagacata
     4921 tcatcggttg caagttttag atgagaaaga ttttcaaagt cctcccacca aactttccct
     4981 tcgtctgaag actggagttc accagtaaag tgttctgtct tgtaaaaaag gacgacataa
     5041 cgataatcct tgtcgtcata ccagtttttg ataccacaga gttggggttt ggaaatgatc
     5101 agaccagttt cttctttcac ttcacgaatg acagcatcga caaaggattc gccacgttca
     5161 acatgaccac caggaaaagt aatgccaggc cagtcgggac taactcggtc ttggaccagg
     5221 accttatctc cgtttttaat catacacatg ttaacaaatt cgactgcctc tcttctgttc
     5281 attcttcaca acctttaatc tttaatcata atgcagactt cccgccaccc agccggtaca
     5341 gagggcagaa gtgatgttaa agccacccgt gtgggcattg atatccataa cttcgcctgc
     5401 aaagtggagg ccaggtacca gcttactttc aagggtttta ggattgattt ccttgagact
     5461 gactccaccc ttggtaacaa aggactttgc aagggacatt tttccagtta caggaatttt
     5521 aagttcttta atggactgga caagctgttc tcgttccttt tcagtcagct gtttgacttt
     5581 ttcaggatat ccttgtacaa aaaattcggc caagcgttct ggtaacaagg tttttaaagc
     5641 gtttttcaag gatttttccc gattttttct agaaatgtaa ccaagttctt ctcagaaagt
     5701 tgaggcaaaa catcgagtga gagaacctcc ccaccgttta caaagctaga catgcgtagg
     5761 gcagcaggac ctgacaaacc aaagtgggta aagagtaaat catgagtgat gacatgctta
     5821 ccataactta gggtcacatc gtccagagaa atcccttgta aggctttatg tggaaaatct
     5881 gttaataaag gactttcagc agcctcaaga tctgtgatgg tatgcttaaa atggcgagca
     5941 atctcgtgac caaaaccagt cgaaccagtc gaaggataag acttaccacc tgttgtgaca
     6001 atgagtttct cacaagtgaa ggtttgatcc gctgacttaa ggacaaactg gtcatctact
     6061 tttttaacag aaacgatttc tatttgagta gcaacttgac cacctagttc ggtgattttc
     6121 ttttccaaag cttcgataat agtccgagac ttgtcactgg ctggaaagac gcgtccgtgg
     6181 tcttcgacct taagtttaac accattttct gtaaaaaagt tgatgatgtc atgattatcg
     6241 aactgggaga aaacactgta aagaaagcgt ccgtttccag gaattccagc tagcaggttg
     6301 tctaagctac cattgttggt cacattgcaa cgtcccccac cagtcccagc taattttttt
     6361 ccaagtttcc gatttttttc gatgaggagg gttttctgtc cataaaagct actggaaatc
     6421 gtagccatca taccagcagg tcccccaccg atgacaatag tatcaaaatg tttcatagct
     6481 ctattgtacc acaaaaaaac aagagatgat ggtcacctct tgtcaagaat gcaattaatc
     6541 aatttcatag cccatcagca aaccgccatc ttctgcatag aaactgcaga gaccagaggt
     6601 tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg attcgctctg agagctgttg
     6661 acaacatttt tcgttattgc gttgggccat gacaatacgg ccaccagcat atccagcttt
     6721 tactaactca tcataggcag cttgaattga tttctttgat ccccttgctt tttgtagcaa
     6781 ttcgagagtt ccagtttcac tagcttttcc gaccatacga atgttgagaa ggccaacgac
     6841 cgtaccgata agcttgctca aacggccgtt cttcaccaag ttatcgactt tggctaggac
     6901 aaagagcaac ttagtttttt cttgataggc ggtgatagct tcaaccactt cttcaaaaga
     6961 caagccctgg tcaatcaagt cattcaattt ttctacgagt aggtcaactt caccaccagc
     7021 agataaacta tcaatcacat gaatcttagt gtcaggatgg tcttccagat aaatattctt
     7081 tgctagttga gcactattgt gactgccaga aagggtacct gtgatggtta ctaggaaaat
     7141 gtttttggca ccttcaaatg ctcgcaaata gtcatctggg cttggacaag ccgattttga
     7201 agcttctgca gttgcataca tggtttccat catttggtca atatcgagac tggcgtcatc
     7261 aacaaagacc tgatcagcta cttgaatggt taaggggacg cttacaaagg ttgtgttaat
     7321 agctggtgtt ggcagttgac gataatcaca accagagtca gcaataatct tccaagtcat
     7381 agaaattctc catctttgtc agttatacat tgacaaaggt tctgtctttt tttacaatta
     7441 tatcatgaaa acccttgaaa caaaagcatc atccgtctgt tgtgacaagt agaaagaaaa
     7501 tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa aatctcagaa aatcaaacca
     7561 agaatccaat ttattaacca gagaagccat tgaaacagcc ctcttgcaac tcttggaaaa
     7621 aaaggaactg accaagatta gtatttctga attggtcaaa cgtgcaggtg tttcgcgtgc
     7681 ggccttttat cgcaattatg actccaaaga ggaaatttta gagagcgtct ttaaacgaac
     7741 tgtccacaat attatggaac agctgcatca ttacgattta aagacagacc tttatttggt
     7801 ttgggttcac cttttccggg aggccagaaa ggaagctaga gtaattcaat tggccttgga
     7861 ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa tttctagaaa aataccatgg
     7921 gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg agctcagcca tcgtctctgt
     7981 ccttctaaaa tggatcaagg atggcatgaa ggtaccagct gaaaagattg cagatttagg
     8041 tttaccattt tttaaaaaat agagaaaaag gagaaaagag atgactgaaa aaagactggc
     8101 ctgggatgag tattttgcag cccaagccct actaattgcg aatcgttcca cttgtaaacg
     8161 tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt atttccactg gttacaatgg
     8221 ttcggtgtca gggactgagc attgtattga tcacgaatgt ttggtcattg aaggccactg
     8281 tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa ggtgctgagc gtggtgttcc
     8341 caaaggcttt acagcctatg taacccattt tccttgtctc aactgtacaa aacaattgct
     8401 tcaggtcggc tgcaagcgtg tggtttatat caaccagtac cgaatggacg actacgccca
     8461 atacctttat caagaaaaag ggacagaatt gacccattta ccacttgaga cagtacagac
     8521 agctcttaaa gaggctgatc taatgtaaaa attatcaaaa ataaatggtt tagaaagatt
     8581 tttaaaccgt tttttggtat aataagaaga ataaattgaa agaaggaatt ccaaaaatgg
     8641 gaaaaattga agttattaat cacccactga ttcaacacaa attgtcaatc ttgcgtcgta
     8701 cagatacttc tacaaaagct tttcgtgagc tagtagatga gattgcaatg ttgatggggt
     8761 atgaagtact tcgtgatctt ccactagaag atgtggaaat cgaaacacca attacaaaaa
     8821 cagttcaaaa acaattggca ggtaagaaat tggccatcgt cccaatcttg cgtgcaggta
     8881 tcgggatggt tgatggtctc ttgagcttgg ttccagctgc taaagttggc cacatcggta
     8941 tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt ggtgaaattg cctgaggaca
     9001 ttgaccaacg tcaaattttt gtagtagacc caatgttggc aacaggtggc tcagcaatct
     9061 tggctgttga ttctcttaaa aaacgtggcg catcaaatat caaatttgtc tgccttgtat
     9121 ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc agatgtagaa atctttacag
     9181 cagccttgga tgaacgtttg aacgaacacg gttatatcgt tccaggtctt ggagatgctg
     9241 gagaccgctt gttcggtaca aaataagatc gaaaagaaga atgacttgga aaagtatttc
     9301 cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg aaagcagagc aaaaatttga
     9361 ccttttttga ccaagatatt ataatagtct tatcttcagt catttgacca acaaaattaa
     9421 aactcaaaag gagaaatgaa tgattcctgt agttattgaa caaacaagcc gtggagaacg
     9481 ttcttacgat atttactcac gtcttctcaa agaccgcatc attatgctga caggtccggt
     9541 tgaagacaat atggctaact ctgttattgc ccaactgctt ttcttggatg cccaagatag
     9601 tacaaaagat atttaccttt atgtcaatac accaggtggt tctgtttcag ctggtttggc
     9661 aatcgtagat accatgaact ttatcaaggc agatgtccaa accattgtta tgggaatggc
     9721 tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa ggcaaacgtt tcatgcttcc
     9781 aaatgctgaa tacatgattc accaaccaat gggcggtaca ggtggtggta cccaacaaac
     9841 tgatatggct atcgctgcag aacacttgct caaaactcgt aataccttgg aaaaaatctt
     9901 ggctgaaaat tcaggtcagt caatggaaaa agtccatgca gatgcagaac gtgataactg
     9961 gatgagcgcc caggaaacac ttgaatatgg ctttattgat gaaattatgg ccaacaattc
    10021 attgaactaa tgatgataga aggcaaactc gactgggttt gctttttttg gtataatagg
    10081 gagagatttc ttagaaagag gatttatcat gtttgaaaaa gtcaatcgct ctggcttgat
    10141 tatctatctt tactataatc gtgatgccaa aaaactgcag gattatggag atattaccta
    10201 tcattccaag aaacatcgtt acttacaact ctatgttcca actcaagaag tggagcaatt
    10261 ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt agggtttgtc atatccaaga
    10321 gttggaaaca ccctttgtgg gcaatcttta tcgataggaa aacgttatca tcgaaaaagt
    10381 tcaaggaaaa cattgacaat tttctgataa ttcggtatat tcttaacaga ctatttaaga
    10441 aataaggaga caaaaaagat gaagaaaaaa tttgccctat cgtttgtggc gcttgcaagt
    10501 gtagcacttc ttgcagcctg tggagaagtg aagtctggag cagtcaacac tgctggtaac
    10561 tcagtagagg aaaagacaat taaaatcggg tttaactttg aagaatcagg ttctttagct
    10621 gcatacggaa cagctgaaca aaaaggtgcc caattggctg ttgatgaaat caatggcgca
    10681 ggtggtatcg atggaaaaca aatcgaagta gtcgataaag ataataagtc tgaaacagct
    10741 gaggctgctt cagttacaac taaccttgta acccaatcta aagtatcagc agtcgtagga
    10801 cctgcgacat ctggtgcgac tgcagctgcg gtagcgaatg ctacaaaagc aggtgttcca
    10861 ttgatctcac caagtgcgac tcaagatgga ttgactaaag gtcaagatta cctctttatt
    10921 ggaactttcc aagatagctt ccaaggaaaa attatctcaa actatgtttc tgaaaaatta
    10981 aatgctaaga aagttgttct ttacactgac aatgccagtg actatgctaa agggattgcc
    11041 aaatctttcc gcgagtcata caagggtgaa atcgttgcag atgaaacttt cgtagcaggt
    11101 gacacagact tccaagcagc ccttacaaaa atgaaaggga aagactttga tgctatcgtt
    11161 gttcctggtt actataatga ggctggtaag attgtaaacc aagcgcgtgg tatgggaatt
    11221 gacaaaccaa tcgttggtgg tgatggattc aacggtgagg agtttgtaca acaagcaact
    11281 gctgaaaaag catcaaacat ctactttatc tcaggcttct caactactgt agaagtttca
    11341 gctaaagcta aagccttcct tgacgcttac cgtgctaagt acaatgaaga gccttcaaca
    11401 tttgcagcct tggcttatga ttcagttcac cttgtagcaa acgcagcaaa aggtgctaaa
    11461 aattcaggtg aaatcaagaa taaccttgct aaaacaaaag attttgaagg tgtaactggt
    11521 caaacaagct tcgatgcaga ccacaacaca gtcaaaactg cttacatgat gaccatgaac
    11581 aatggtaaag ttgaagcagc agaagttgta aaaccataat agaaaaatgt tgaaacaggg
    11641 aatgagcctt tgactcactc cctgtttcga tatttaatac tcttcgaaaa tctcttcaaa
    11701 ctgcgtcaac gtcgccttgg attatatatg tgactgactt cgtcagtctt atctacaacc
    11761 tcaaagcagt gctttgagca acctgcggct agtttcctag tttgctcttt gattttcatt
    11821 gagtataaga acctatcaaa aagtgaggga aaaccctcgg aattataaat agaaagagtg
    11881 aatcttatgc tccaacaact cgtaaatggt ttgattctag gtagtgttta cgcgctgtta
    11941 gccctaggat ataccatggt ttacggaatt atcaagctca tcaacttcgc ccatggtgat
    12001 atttatatga tgggagcctt tatcggttat ttcttgatca attctttcca aatgaatttc
    12061 tttgtagcgc ttattgtagc tatgctagcg acagctattc ttggtgtcgt gattgagttt
    12121 cttgcttacc gacctttgcg ccactctact cgtattgctg ttttgattac ggctattggg
    12181 gtttctttcc tattggagta tggaatggtc tatctggttg gtgccaatac ccgtgccttc
    12241 cctcaagcga ttcaaacagt tcgatatgat ttgggaccaa ttagcttaac aaatgtgcag
    12301 ttaatgattt tgggcatttc cttgattttg atgattttgt tacaagtcat tgtccaaaag
    12361 actaagatgg ggaaagccat gcgtgcagta tcagtagata gcgacgcggc gcaattgatg
    12421 gggatcaatg taaaccgtac gattagcttt accttcgctt tgggttctgc tcttgcgggt
    12481 gcggctggtg ttctgattgc tctttattat aactctcttg agcctttgat gggggttact
    12541 ccaggtctta aatctttcgt tgccgcagta cttggtggta tcggaattat tcctggtgcg
    12601 gctcttggtg gctttgtgat tggtctattg gaaacctttg cgacagcctt tgggatgtca
    12661 gatttccgtg atgccattgt ttatggaatc ttgttgttga tcttgattgt ccgcccagct
    12721 ggtatccttg gtaagaatgt gaaagagaag gtgtaaacga tgaaggaaaa tttaaaagtt
    12781 aatattctat ggttactcct tttgttagct ggctatagct tgattagtgt actggtttca
    12841 gtcggagtac ttaatctatt ctatgtacag attttacaac aaattggaat taatattatt
    12901 ttggctgttg gtctcaactt aatcgttggt ttttcaggac aattttcact tggtcatgct
    12961 ggtttcatgg cgattggtgc ctatgcagca gctattattg gttctaaatc accaacctac
    13021 ggtgccttct ttggagctat gcttgtaggg gctttgcttt caggagcagt tgccttactt
    13081 gtcggcattc caaccttgcg cttgaagggg gactatcttg cggtagcaac tctgggtgtt
    13141 tctgaaatta tccgtatctt tatcatcaat ggtggaagtc ttacaaatgg tgcggcaggt
    13201 atcttaggga ttcctaactt tacaacttgg caaatggttt acttctttgt cgtgattaca
    13261 accattgcaa ccttgaactt cttgcgtagc ccaattggcc gttcaaccct ctctgttcgt
    13321 gaagatgaaa tcgctgctga gtcagttggg gttaatacga ctaaaattaa aatcatcgct
    13381 tttgtctttg gtgccattac tgcaagtatt gctgggtcac ttcaggcagg atttatcggg
    13441 tctgttgtac cgaaagatta caccttcatc aactcaatca acgttttgat tattgttgta
    13501 tttggtggac tcggttccat tacaggtgcg attgtttcgg ctattgttct gggaattttg
    13561 aatatgcttc tccaagatgt tgctagtgtg cgtatgatta tttacgcttt ggccttggta
    13621 ttggtaatga ttttcagacc aggtggactc cttggaacgt gggaactgag cctatcacgt
    13681 ttctttaaaa aatctaagaa ggaggaacaa aactaatggc attacttgaa gtaaaacagt
    13741 taaccaaaca ttttggtggt ctaacagctg ttggagatgt gactcttgaa ttgaacgaag
    13801 gggaactggt tggattaatc ggtccaaacg gagctgggaa aaccaccctt ttcaaccttt
    13861 tgaccggtgt ttatgaacca agtgagggaa cagtaaccct agatggtcac cttttgaatg
    13921 ggaaatcacc ttataagatt gcctctttgg gacttggacg tactttccaa aatatccgtc
    13981 tttttaaaga tttaacagtt ttagacaatg ttttgattgc ttttggaaac catcacaaac
    14041 agcatgtttt tactagtttc ttacgcttac cagcttttta caagagtgaa aaagaattaa
    14101 aggctaaagc tttggaattg ttgaaaatct ttgatttaga tggtgatgca gagactcttg
    14161 ctaaaaatct ttcctacgga caacaacgtc gtttggaaat tgttcgtgcc cttgctacgg
    14221 aacctaaaat tctcttctta gatgaaccag cagcaggtat gaacccacag gaaacagccg
    14281 aattgactga gttaattcgt cgtatcaaag atgagtttaa gattacaatc atgttgattg
    14341 aacacgatat gaatctggtc atggaagtaa cagaacgtat ctacgtactt gaatatggcc
    14401 gtttaatcgc tcaaggaact ccagacgaaa ttaagaccaa taaacgcgtt atcgaagctt
    14461 atctaggagg tgaagcctaa tgtctatgtt aaaagttgaa aatctttctg tgcattacgg
    14521 tatgatccaa gcagttcgtg atgtaagctt tgaagttaat gaaggagaag ttgtttccct
    14581 tatcggtgcc aacggtgcag gtaagacaac tattcttcgc accttgtcag gtttggttcg
    14641 accaagttca ggaaagattg aatttttagg tcaagaaatc caaaaaatgc cagctcagaa
    14701 aatcgtggca agtggtcttt cacaagttcc agaaggacgc cacgtctttc ctggcttgac
    14761 tgttatggaa aatcttgaaa tgggagcttt cttaaagaaa aatcgtgaag aaaatcaagc
    14821 taacttgaag aaggttttct cacgctttcc tcgtcttgaa gaacggaaga accaagatgc
    14881 agccactctt tcaggggggg aacaacaaat gcttgccatg ggacgcgccc tcatgtcaac
    14941 accaaaactt cttcttttag atgaaccatc aatgggactt gccccaatct ttatccaaga
    15001 aatttttgat atcattcaag atattcagaa gcaaggaaca acggtcctct tgattgaaca
    15061 aaatgccaat aaagcacttg caatctctga ccgaggatat gtactggaaa cagggaaaat
    15121 cgtcctatca ggaacaggaa aagaactcgc ttcatcagaa gaagtcagaa aagcatatct
    15181 aggtggctaa aacaatccag tggattgttt tagtcggcag atggagatta cgaagtaatc
    15241 atcaatatag tccgggggac ctttttagtc ggtagattga gattgcaaac aaatctgcat
    15301 ctacattgaa agcttaattt ctaataattg aaaaaatcga atgaaaaatt tcttaccttc
    15361 attcacagag ctcgatttca gagctctttt tgctagctta ttcatacttt tttgaatttc
    15421 gaaaaagaaa tgtaagcgtt tgatagattt acaaaaagat tgtataatgg ggataagaat
    15481 agaaaaggag aagtctcatg gcagttaaag attttatgac ccgcaaggta gtttatatta
    15541 gtccagatac aacagtatct catgcagcag atttgatgag agagcaagga ttgcaccgct
    15601 tgcctgttat cgaaaatgat caattggttg gtttggtgac tgagggaacc attgcacaag
    15661 caagtccatc taaagcaaca agtctttcta tctatgagat gaattatctt ctgaataaga
    15721 caaaagtaaa agatgtcatg attcgcgatg ttgtcactgt ctcaggctat gctagtctag
    15781 aagatgcaac ttatctgatg ttgaaaaata agattggtat tctccctgtc gtagataacc
    15841 atcaagtata cggagttatt actgaccgtg acgttttcca agcctttctt gaaattgcag
    15901 gttatggcga agaagggatt cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg
    15961 gaaaaattat ttctttgatt gtagaagaaa atttgaatat atcccataca gtcaatattc
    16021 cgcgtaagga tggtaaggtc attatcgaag tgcaaatcga tggatcaatt gatttaccag
    16081 ccttgaaaga aaaatttgaa gcaaatggta ttcaagtgga agaaatcgct cgcacttcag
    16141 caaaagtctt gtaagaaggg aagccaaagg cttctttttt catgaaaagg ggattagagc
    16201 aaaagatgga aagaaatgat aaaatatgct ataatgaaat aatgtaaaaa aggagtattt
    16261 atggacattt cagtaattcg tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc
    16321 agggggtctc tttgacctcg aagggctaga ggaagagatt gccatcttgg aaaacaagat
    16381 gacagaacct gatttttgga acgataatat tgcggcccaa aaaacgtcgc aagaattgaa
    16441 tgaattaaaa aacacttaca ataccttcca taagatggaa gagttgcagg atgaagtcga
    16501 aattttattg gattttttgg ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt
    16561 agccgaactt gataagataa tgactagtta cgagatgact ctactcttgt cagaacctta
    16621 tgaccacaac aatgccatct tggaaatcca tccaggttct ggtggtactg aggcgcagga
    16681 ctggggtgat atgttgcttc gtatgtatac tcgttatggt aatgctaaag gctttaaagt
    16741 ggaagtgttg gattaccaag caggtgatga ggctggtatt aagtcggtaa ctttatcatt
    16801 tgaagggcct aatgcctatg gtctcctcaa gtcagaaatg ggtgttcatc gcttagtgcg
    16861 aatctcacca tttgactctg ccaaacgtcg ccatacctct ttcacatctg tagaagtgat
    16921 gccagaattg gatgatacta ttgaagtgga aatccgtgaa gatgatatca agatggatac
    16981 cttccgttca ggtggtgccg gtggacaaaa cgtcaataaa gtttcaacag gtgtacgttt
    17041 aacccacatt ccaactggaa ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa
    17101 tagagatcgt gccatgaaga tgttgcaggc taagctctat caaatggagc aagataagaa
    17161 ggctgcggag gtagattctc tcaaaggtga gaaaaaggag atcacttggg gaagccaaat
    17221 ccgttcttat gtcttcacgc cttatactat ggtaaaagat caccgaacta gctttgaggt
    17281 tgctcaggta gataaggtta tggatgggga cctagatggt tttatcgatg cttatctcaa
    17341 gtggcgaatt agctaagata gaaaggaact cacatgtcaa ttattgaaat gagagatgtc
    17401 gttaaaaaat acgacaacgg aacgactgct ctacgcggtg tttcggttag cgttcaaccg
    17461 ggggaatttg cctacatcgt aggaccttca ggagcaggga agtcaacttt tattcgttct
    17521 ctgtatcgtg aagtaaaaat cgataaagga agcctatcag ttgctggttt taatctggtt
    17581 aagatcaaaa agaaagatgt cccgcttcta cgtcgtagtg ttggggttgt cttccaggat
    17641 tataaactgt taccaaagaa aactgtctat gaaaatattg cttacgctat ggaagtaatc
    17701 ggggaaaatc gccgtaatat caaaagacga gtgatggaag ttttggactt ggttggattg
    17761 aagcataagg ttcgttcttt tccaaatgaa ctctcaggtg gggagcagca gcggattgcg
    17821 attgcgcgtg caattgtaaa taatcccaaa gtattgatag ctgatgagcc aacaggaaat
    17881 ctggatccgg ataattcatg ggaaattatg aatctcttgg aacggattaa cctacaagga
    17941 acaactattt tgatggcgac tcataatagc cagattgtaa ataccttgcg ccaccgtgtc
    18001 attgccattg aaaatggccg tgtcgttcgt gacgaatcaa aaggagagta tggatacgat
    18061 gattagtaga ttttttcgcc atttatttga agccttaaaa agtttgaaac gaaatggttg
    18121 gatgacagta gctgctgtca gttcagtcat gattactttg accttggtgg caatatttgc
    18181 atctgttatt ttcaatacag cgaaactagc tacagatatt gaaaataatg tccgtgtagt
    18241 agtttatatc cgaaaggatg tggaagataa tagtcagaca attgaaaaag aaggtcaaac
    18301 tgttacaaat aatgactacc acaaggtata tgattctttg aagaacatgt ctgcggttaa
    18361 aagtgttacc ttttcaagta aagaagaaca atatgaaaaa ttaaccgaga taatgggaga
    18421 taactggaaa atctttgaag gagatgccaa tcctctctat gatgcttata ttgtagaggc
    18481 aaacactcca aatgatgtaa aaactatagc cgaagatgct aaaaaaattg aaggtgtctc
    18541 tgaggttcaa gatggcggtg ccaatacaga aagactcttc aagttagctt catttatccg
    18601 tgtttgggga ctagggattg ctgctttgtt aatttttatc gcagttttct tgatttcaaa
    18661 taccattcgt attaccatta tttcccgcag tcgcgaaatt caaatcatgc gcttggtcgg
    18721 agctaaaaac agttatatcc gtggaccgtt cttgttagaa ggagccttta tcggtttatt
    18781 gggagctatc gcaccatctg ttttggtctt tattgtttat caaattgttt accaatctgt
    18841 caacaaatcg ttggtagggc aaaatctatc catgattagt ccagatttat ttagtccgtt
    18901 gatgattgcc ctactatttg tgattggggt tttcattggt tcattgggat caggaatatc
    18961 catgcgccga ttcttgaaga tttaggtaaa atagctgctt ttatgaggag attgtaaaat
    19021 ctcctttttt gctacaagag tttttgaaaa gagattccaa agaagtcccc cagagaagac
    19081 ttcttgatgg ctaatttgaa cttgaaattt ggcgcactag aaatttttta gaaaaaagtg
    19141 taattttttt ataaaagcct ttacaaaaaa aattaaagtg gtataattaa atcatagaaa
    19201 gtgcaaacgt tttcgtaaaa cgtttgccta aataaaaata aaggagattt gaatgatgaa
    19261 agatacattc aaaaatgtct tgtctttcga attttggcaa aaattcggta aggctttgat
    19321 ggtagttatc gcggttatgc cggctgctgg tttgatgatt tcaatcggta agtctatcgt
    19381 gatgattaac ccaacctttg caccacttgt catcacaggt ggaattcttg agcaaatcgg
    19441 ttggggggtt atcggtaacc ttcatatttt gtttgcccta gccattggag gaagctgggc
    19501 taaagaacgt gctggtggtg ctttcgccgc tggtcttgcc ttcatcttga ttaaccgtat
    19561 cactggtaca atctttggtg tatcaggcga tatgttgaaa aatccagatg ctatggtaac
    19621 tactttcttt ggtggttcaa tcaaagttgc tgattacttt atcagtgttc ttgaagctcc
    19681 agccttgaac atgggggtat tcgtagggat tatctcaggt tttgtagggg caactgctta
    19741 caacaaatac tacaacttcc gtaaacttcc tgatgcactt tcattcttca acgggaaacg
    19801 tttcgtacca tttgtagtta ttcttcgttc agcaatcgct gcaattctac ttgctgcttt
    19861 ctggccagta gttcaaacag gtatcaataa tttcggtatc tggattgcca actcacaaga
    19921 aactgctcca attcttgcac cattcttgta tggtactttg gaacgtttgc tcttgccatt
    19981 tggtcttcac cacatgttga ctatcccaat gaactacaca gctcttggtg gtacttatga
    20041 cattttaact ggtgcagcta aaggtactca agtattcggt caagacccac tatggcttgc
    20101 atgggtaaca gaccttgtaa accttaaagg tactgatgct agtcaatacc aacacttgtt
    20161 agatacagta catccagctc gtttcaaagt tggacaaatg atcggttcat tcggtatctt
    20221 gatgggtgtg attgttgcta tctaccgtaa tgttgatgct gacaagaaac ataaatacaa
    20281 aggtatgatg attgcaacag ctcttgcaac attcttgaca ggggttactg aaccaatcga
    20341 atacatgttc atgttcatcg caacacctat gtatcttgtt tactcacttg ttcaaggtgc
    20401 tgccttcgct atggctgacg tcgtaaacct acgtatgcac tcattcggtt caatcgagtt
    20461 cttgactcgt acacctattg caatcagtgc tggtattggt atggatatcg ttaacttcgt
    20521 ttgggtaact gttctctttg ctgtaatcat gtactttatc gcaaacttca tgattcaaaa
    20581 attcaactac gcaactccag ggcgtaacgg aaactacgaa actgctgaag gttcagaaga
    20641 aaccagcagc gaagtgaaag ttgcagcagg ctctcaagct gtaaacatta tcaaccttct
    20701 tggtggacgt gtaaacatcg ttgatgttga tgcatgtatg actcgtcttc gtgtaactgt
    20761 taaagatgca gataaagtag gaaatgcaga gcaatggaaa gcagaaggag ctatgggtct
    20821 tgtgatgaaa ggacaagggg ttcaagctat ctacggtcca aaagctgaca ttttgaaatc
    20881 tgatatccaa gatatccttg attcaggtga aatcattcct gaaactcttc caagccaaat
    20941 gactgaagca caacaaaaca ctgttcactt caaagatctt actgaggaag tttactcagt
    21001 agcagacggt caagttgttg ctttggaaca agtaaaggat ccagtatttg ctcaaaaaat
    21061 gatgggtgat ggatttgcag tagaacctgc aaatggaaac attgtatctc cagtttcagg
    21121 tactgtatca agcatcttcc caacaaaaca tgcttttggt attgtgacgg aagcaggtct
    21181 tgaagtattg gttcacattg gtttggacac agtaagtctt gaaggtaaac catttacagt
    21241 tcatgttgct gaaggacaaa aagttgcagc aggagatctc cttgtcacag ctgacttgga
    21301 tgctatccgt gcagcaggac gtgaaacttc aacagtagtt gtcttcacaa atggtgatgc
    21361 aattaaatca gttaaattag aaaaaacagg ttctcttgca gctaaaacag cagttgctaa
    21421 agtagaattg taatatactt gaggttggaa gctgtattcc aacctcttat tttgggagaa
    21481 aagaatgaaa tttttaacac tcaatactca cagttggatg gagaaagaag cagaggaaaa
    21541 attccagatt ttgcttgaag atattcttga aaaggactat gatttgattt gttttcaaga
    21601 aatcaatcag gagatgacct cgtcagaggt ggaggttaat gacctttatc aagctttgcc
    21661 agcagctgag cctattcacc aagaccatta tgttagactc ttggttgaaa agttgttaga
    21721 gcaagggaaa aattactact ggacctgggc ttataaccat atcggctatg accgctacca
    21781 cgaaggtgtg gctatcttgt ctaaaacacc tattgaagcc agagaaattt tggtttcaga
    21841 tgtggatgat ccaacagact atcatactcg ccgtgttgcc ctagctgaaa ctgtagttga
    21901 tggcaaggag ctagcagttg ccagtgttca tctctcttgg tgggataaag gtttccaaga
    21961 agaatgggca cgatttgagg ctgttttgaa aaaattgaac aagccacttt tactagctgg
    22021 agattttaac aacccagcag gtcaggaagg ttaccaagct attttagcta gtccattagg
    22081 cttacaagac gcatttgaag ttgctcaaga gaaaagtggt agctatactg ttccgcctga
    22141 aattgatggc tggaaaggga acactgaacc ccttcgaatc gattatgtct ttactaccaa
    22201 agagttagcg gtggaaaatt tacatgtcgt atttgatggt aacaagagtc cacaagtgag
    22261 tgatcactat ggcttgaatg ctatgttaaa ctggaaataa taactgaaaa gaggttggaa
    22321 ctataaaatt ccagcctttt cttactagag aagctactgg aaatagccta aataagtgag
    22381 actactgtaa tggaataaaa tatggtataa ttgataagat agatagaatc gaggatgtta
    22441 tgtcatttac gaaatttcaa tttaaaaact atattagaga agccttgaag gagttaaaat
    22501 ttacaactcc aacagaggtg caagacaagt tgattcctat tgttttggca ggtcgtgacc
    22561 tagtaggaga atcaaaaaca ggttcaggta agactcatac tttcttgtta ccgattttcc
    22621 agcaattaga tgaagctagc gatagtgtgc aagcagtgat tactgcaccg agtcgtgagt
    22681 tggctactca aatttaccaa gtagcgcgtc agatttcagc tcactcagat gtcgaagttc
    22741 gtgtggttaa ttatgtgggt ggtacggata aggctcgcca gattgagaaa ttggcaagca
    22801 atcagcctca tattgttatt ggaacaccag gccgtatcta cgacttggtt aaatctggtg
    22861 atttagctat tcataaagcc aagacatttg ttgttgatga agcagatatg accttggata
    22921 tgggattctt ggaaactgtt gataagattg ctggcagtct tccaaaagac ttgcaattca
    22981 tggtcttctc agcgactatc ccacaaaaac tgcaaccatt cttgaaaaaa tacttatcaa
    23041 atcctgttat ggagaaaatt aagaccaaaa cggttatttc tgacaccatt gataattggt
    23101 tgatttcgac caagggacat gataagaatg ctcaaattta ccagttgact cagttgatgc
    23161 agccgtattt ggcaatgatt tttgttaaca ctaaaacgcg tgctgatgaa ttgcattcat
    23221 atctgactgc tcaaggcttg aaggttgcaa aaatccatgg agatattgcc cctcgtgaac
    23281 gcaagcgaat catgaatcag gtgcaaaatc tggattttga gtatattgtc gcaacagatt
    23341 tggcagcgcg tgggattgac attgaaggtg tcagccatgt catcaatgat gccattccgc
    23401 aagacttatc tttttttgtt catcgtgttg gtcgtactgg acgaaatggc ctaccaggta
    23461 cagctattac cctttatcag ccaagtgatg actcggatat ccgtgagttg gagaaattgg
    23521 aaatcaagtt tagtcctaag atggtcaaag acggggaatt tcaagatacc tatgaccgtg
    23581 atcgtcgtgc caaccgtgag aaaaaacaag ataaacttga tatcgaaatg attggtttgg
    23641 ttaaaaagaa aaagaaaaaa gtcaaaccgg gttataagaa gaaaattcaa tgggcggttg
    23701 atgaaaagcg ccgtaaaacc aagcgtgctg aaaatcgcgc tcgcggtcgt gcagagcgta
    23761 aagctaaacg ccaaacattt taatagaaat tgttggagta ttgagctcca actttttgct
    23821 ttatgagaac gaactatcta aaccgaaaca ctacattaaa gactgcaaat tgcgattaaa
    23881 aatggtataa tgataaagtt atatagtccc gataagatgg taggtattta ttacgaagag
    23941 ttttcctatc agtactttgt aactctataa caatattttt taagggggga catttttatg
    24001 tcagaacgta aattattcac gtctgaatct gtatctgagg ggcatccgga taagattgca
    24061 gaccaaattt cagatgcgat tttggatgct attttagcaa aggatccaga ggcgcacgtt
    24121 gctgctgaaa cagctgtata tactggttct gtccacgttt ttggtgaaat ttctacaaat
    24181 gcctatgtgg atattaaccg tgtggttcgt gataccattg cagagattgg ttataccaat
    24241 acagaatatg gattttctgc tgagacggtg ggagtacacc catctttggt ggaacaatct
    24301 cctgacatcg ctcaaggtgt taacgaagcc ttggaggttc gtggaaatgc tgatcaagat
    24361 ccactggact tgattggagc aggtgaccaa gggctcatgt ttggatttgc agtagatgaa
    24421 acagaagagc tcatgccatt gccaattgca ctcagtcata aattggttcg tcgtctggca
    24481 gaacttcgta agtctggaga aattagctat ctccgtccag atgcaaaatc acaagttaca
    24541 gttgagtacg atgaaaatga ccgtccggta cgtgtagata cagtcgttat ttctactcag
    24601 catgatccag aggccactaa tgaacaaatc catcaagatg tgattgacaa ggtcatcaaa
    24661 gaagttattc catcttctta tcttgatgat aagacaaaat tctttatcaa tccgacaggt
    24721 cgttttgtaa tcggtggtcc tcaaggggac tcaggtttga ctggtcgtaa gattattgta
    24781 gatacttatg gtggctactc tcgtcatggt ggtggtgcct tctctggtaa agatgcgact
    24841 aaggtggatc gttcagcctc ttatgcggct cgctatattg ccaagaatat cgttgcagca
    24901 gaccttgcta agaaggcaga agtgcagttg gcctatgcta tcggtgttgc gcaacctgtt
    24961 tctgttcgta tcgatacttt cggtacagga acagtagctg aaagtcaact tgaaaaagcg
    25021 gctcgtcaaa tctttgacct tcgccctgca gggattatcc aaatgctgga ccttaagcgt
    25081 ccaatttacc gtcaaacatc ggcttacggt cacatgggac gtacagatat tgatcttcca
    25141 tgggaacgtt tggataaggt agatgctttg aaagaagcag taaaataaga ttttaagagg
    25201 ggaacgtcct ctctttttta tagtttttaa ctatactggg atactgttct gaaaatccat
    25261 tttgcgaaag tagagattta catgtatagt agattgaaac taaaatagta cacttgaact
    25321 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttctcctg ttcttgtttc
    25381 attttactat atttctttaa aaatgataaa ggttgagatt tctccttgtt gcttggactt
    25441 cattcaaaag tctgttaccc aagccttgtt caaacttcca atacactagc tgtttccata
    25501 gcatgacttc tgtactagac tttcttttcc gaataaatag atagaaccac agaatctagt
    25561 aaacctagaa ttaaaattat ggtataatat tagcaataaa agaaatctgg aggattagaa
    25621 tcatggtatc aacgaaaaca caaattgctg gttttgagtt tgacaattgc ttgatgaatg
    25681 cagcaggtgt ggcttgtatg acgataaagg agttagaaga ggtcaaaaac tcagcggcag
    25741 gaacctttgt tactaagaca gcgaccttgg atttccgtca ggggaatcct gagccacgct
    25801 accaagatgt tccacttggt tccatcaact ctatgggctt gccaaataat ggcttagact
    25861 attatttgga ttatctttta gatttgcagg aaaaagagtc gaaccgaact ttcttcttat
    25921 ctctggtcgg catgtctcca gaggaaaccc atactatttt gaaaaaagtc caagagagtg
    25981 attttcgtgg tctgactgag ctaaatcttt cctgtccaaa tgttccaggt aaacctcaga
    26041 ttgcctatga ttttgagaca acagaccgga ttttggcaga agtgtttgct tacttcacca
    26101 aacctcttgg aattaaattg ccaccttatt ttgatattgt tcactttgac caagcggcag
    26161 ctattttcaa caaatatccg ctcaagtttg tcaactgcgt taactctatc ggaaacggcc
    26221 tctatataga agacgagtct gtcgttattc ggcctaagaa tggttttggt ggaattggtg
    26281 gagaatacat caaaccgact gctctagcca atgttcacgc cttttatcaa cgtttaaatc
    26341 ctcaaatcca aattatcgga acaggtggcg ttctgactgg tcgagatgcc tttgaacaca
    26401 tcctctgtgg agcaagtatg gtgcaggtgg gaacgaccct tcacaaagaa ggcgtcagtg
    26461 cttttgaccg cattaccaat gaactgaaag caatcatggt ggaaaaaggc tacgagagct
    26521 tagaagattt ccgtgggaaa ttgcgctata ttgactaaat taaatcgaaa aatctgaaga
    26581 aaggagagac gatgctagcc attgaagaaa gtcagaagtt gactttatca aatttaccga
    26641 gcctgagcct atttacaggg acagatcagg gtcagtttga agtgatgaag agtcaaatgt
    26701 tgaaacagat tgggtatgat tctgctgacc tcaactttgc ctactttgat atgaaagaag
    26761 tagtttacaa ggatgtggaa ctggagttgg tcagccttcc tttctttgcg gatgagaaaa
    26821 tcgtgatatt agattatttt atggatatca cgactgctaa gaaacgcttt ttgacagatg
    26881 atgagcttaa gtcatttgag gaataccttg acaatccttc tccaacaacc aagttgataa
    26941 tctttgcaga aggaaagctg gatagcaaaa gacggttagt caaattactt aagcgtgatg
    27001 ccaaggcctt cgatgcagta gaagtaaaag aacaagaatt gcgccagtac ttccaaaagt
    27061 ggagtcagaa acaaggtctg cagtttacca atcattcttt tgaaaatctc ctcatcaagt
    27121 cggggtttca atttagcgaa atccagaaaa atcttctctt tttacagtcc tataaggtgg
    27181 attctgttat cgaggaagag gatattgtta acgcaattcc caagaccttg caggacaata
    27241 tttttgattt aactcagttt attctgacta aaaagatgga tcaggcgcgc gatttggtga
    27301 gagacttgac cttgcaaggg gaagatgaaa tcaaactgat tgcagtcatg ctgggacaat
    27361 ttcggacttt tactcaggtg aagattttgg cggagtctgg ccaaacagaa tcgcagattg
    27421 caagtagttt aggtagttat ctgggacgta acccaaatcc ttaccaaatc aagtttgcat
    27481 taagagattc gagaggactt tctttgagct ttctgaagca agctatttcc tatttgattg
    27541 agacagacta tcagattaag acaggtcttt atgaaaaagg tttccttttt gaaaaggcac
    27601 tcttacagat tgctagtcag gtcaattgac atttgttgaa actacaaccc gcgccaggat
    27661 tatcttgtcg tgggtttctt gctgatttgc ttattttatt agaaagtatg tcagtgattt
    27721 gcttatctat aagcaaaaaa cttgaaaaaa gtgaatgttt gcgttatcat tttcatatag
    27781 aaagaaaaag aggtattaca gatggctatt atcttaccag aacttccata tgcatacgac
    27841 gctttggaac catacatcga tgcggaaaca atgcatttgc accatgacaa acaccatcaa
    27901 acttatgtca acaatgccaa tgcagcttta gaaaaacacc ctgaaatcgg tgaagacctt
    27961 gaagccttgc ttgctgatgt agaatctatc ccagctgata tccgtcaagc acttatcaac
    28021 aatggtggcg gacacttgaa ccacgctctt ttctgggaat tgatgactcc cgagaaaaca
    28081 gctccttcag cagagctggc agcagcaatc gatgcaacat ttggttcatt tgaagaattc
    28141 caagcagcct tcactgcagc agcaacaact cgttttggtt caggttgggc atggttggtt
    28201 gtcaacaaag aagggaaact tgaagtgact tcaacagcaa accaagacac accaatctca
    28261 gaaggtaaaa aaccaatctt gggcttggac gtttgggaac atgcttacta cgtgaaatac
    28321 cgcaacgtgc gtcctgacta catcaaagct ttcttttcag taatcaactg gaataaagta
    28381 gatgaattgt acgcagctgc taaataatga tagttggagg gaagaattgt tcttctcttt
    28441 tttaggttat aagattctgg tctgacaaaa tcgtcagact tttttcattt ttatgagaaa
    28501 cgtgctaact gctagaaatt atggtagaat aaagtattaa gtaatcgtgg aaaaaggata
    28561 tctatgcgaa aagaaattgc acctgaatta tacaactata acaagtttcc tggtccggag
    28621 ttccatttac acggggacaa ggtcgaaacg gaagggatag ctttttcctt ggttgaaaat
    28681 atcaaggatg cctttgatgt gacgactttt aatcagcgtt tttcagaagt attaaccaag
    28741 tttgattata tcgtggggga ctggagcaac gaacagcttc gcctacgagg tttttacaag
    28801 gatgaccgaa cagaagaaaa acttgaaaaa atcagttgtt tacaagacta ccttttagag
    28861 tattgtagtt atggttgtgc ctattttgtc ttagaaaatg aagcccctaa gcgagcatca
    28921 tttgacaaga aaatgcgtaa gacggaagaa gaaacacctt ctagaaaagg aaagaaaccg
    28981 actcaaacta aacgaaagtc gaatgcagat aagaaaaata gacgtcgtca gaaagaccag
    29041 cattctcata aagaggacaa gggacaacgt cattttgtca ttcgtcagaa gtgagtagag
    29101 aataaggaga agaaatgaaa ccctcaattc atagtttggc acatcaaacc atgcaggaat
    29161 gggtattgga gcagggagaa aagaaattcc gtgcagatca aatctgggaa tggctctacc
    29221 gtaaacgtgt gcagtcattt gaagaaatga ccaacctttc caaggatttg attgctaagc
    29281 tcaatgacca gtttgtggtc aatcccttga aacaacgtat tgtgcaagag tctgctgatg
    29341 gtacagtcaa atatctcttt gaattgcctg acggtatgtt aattgaaact gtactcatgc
    29401 gtcaacacta tggtttatct gtctgtgtga ccactcaggt cgactgtaat atcggttgta
    29461 ccttctgtgc ctctgatttg attaagaagc aacgtgacct caataacggt gaaatcgtag
    29521 cgcaaatcat gctggttcag aaatactttg ctgagcgtgg tcaggatgaa cgcgtcagcc
    29581 atatcgttgt catgggaatc ggtgagccct ttgataatta caacaatgtc ttgaatttcg
    29641 tttgtactat caatgatgat aaaggaatgg ctatcggtgc tcgtcacatc acggtttcaa
    29701 cctcaggttt ggctcataaa attcgtaact ttgctgatga aggggttcag gtcaatcttg
    29761 ccgtgtctct tcacgcaccc aacaatgaac ttcgttcaag tatcatgaag attaaccgtg
    29821 cctttccgat tgaaaaactc tttgctgcta ttgaatacta cattgagaca acaaaccgtc
    29881 gtgtaacctt tgaatatatc atgcttaatg aagtcaacga cggtgtagaa caagccttgg
    29941 aattggctga attgctcaag aacatcaaga aattgtctta tgtaaacttg attccttata
    30001 acccagttag tgagcatgac caatatagcc gtagtcccaa agagcgcgtg ttggctttct
    30061 atgatacgct taagaaaaaa ggggtcaact gtgtggttcg tcaagagtat ggtacagata
    30121 ttgacgcagc ttgtggacaa ttgcgctcta acacaatgaa acgtgaccgc cagaaggcag
    30181 tcgcagaggt aaatccgtaa aatgactaaa aaaagagaat taatcttaag attgggagtg
    30241 gctgtttata gcctatgcat tgtctatttt tgctttactc ctcaagctac cgtggaagtg
    30301 ggaactccag gtattcagca tcttggacgc ctggtttttc ttttgactcc tttcaattct
    30361 ctctggaaac tgggcgaagt gagtgacatt ggacaattat gttggatttt tttacaaaat
    30421 atcctcaatg tcttcttgtt ttttcctctg attttccaac tcctttatct atttccaaat
    30481 ttgcggaaaa caaaaaaggt ccttcttttt agttttcttg tgagtcttgg aatcgagtgt
    30541 acgcaattaa tcttggactt tttctttgat ttcaatcgcg tctttgagat tgatgatttg
    30601 tggaccaaca ctttgggtgg ctatctggct tggctccttt ataaacgatt acataaaaac
    30661 aagataagga attaaaatga gtattttaga agttaaaaat ctgagtcacg gttttggtga
    30721 ccgtgcaatt tttgaagatg tgtccttccg tctcctcaag ggagaacata tcggcttggt
    30781 cggtgccaat ggtgaaggaa aatcaacctt tatgagtatc gtgactggta aaatgctgcc
    30841 agatgaagga aaggttgagt ggtccaaata tgtgacggct ggttacttgg atcagcactc
    30901 tgtccttgct gaaagacagt cggtgcgtga tgttctccgt acggcttttg atgagctttt
    30961 caaagctgaa gctcgtatca atgacctcta tatgaaaatg actgaagacg gtgcggatgt
    31021 tgatgctctc atggaagaag taggagaact tcaagaccgt ctggagagtc gtgatttcta
    31081 tatcttggat gctaagattg acgaagtagc gcgtgctctt ggtattatgg actttggcat
    31141 ggatacggat gtaacttctt tgtcaggtgg gcaaagaacc aaggtgcttt tggcaaaact
    31201 tctccttgaa aagcctgata ttttgctgtt ggacgagccg accaactact tggatgctga
    31261 gcatattgat tggctcaagc gctatctcca aaactatgag aatgcctttg ttctcatttc
    31321 gcacgatatt ccattcctca atgacgttat caatattgtc tatcatgtgg aaaatcaaca
    31381 gctgacgcgt tactctggtg actactacca gttccaagaa gtttatgcta tgaaaaaatc
    31441 tcagctagag gcagcctacg aacgccagca gaaagagatt gcagacctca aggactttgt
    31501 ggctcgtaat aaagcccgtg ttgcaactcg taatatggct atgtctcgtc aaaagaaatt
    31561 ggataagatg gatattatcg aactccaaag tgagaaacca aaaccatcct ttgatttcaa
    31621 accagctcgt acaccagggc gctttatctt ccaagccaag aacttgcaaa ttggttacga
    31681 ccgtcctctt actaagtctt taaatcttac cttcgaacgc aatcaaaagg ttgcgattat
    31741 tggtgctaat ggtattggaa aaacaactct cttgaagagt ctcttgggca ttatctcgcc
    31801 aatcgctggg gaagtggagc gtggagatta tttagaactt ggttattttg agcaggaagt
    31861 agaaggtggt aatcgccaaa ctcctcttga agctgtctgg aatgcctttc ctgcccttaa
    31921 tcaagcagaa gtccgtgcag cccttgcccg ttgtggtttg acaaccaaac atattgaaag
    31981 ccagattcaa gtattatcag ggggagagca agccaaggtt cgtttctgtc tcttgatgaa
    32041 tcgtgaaaac aacgttttag tgctggacga gccgaccaac catttggatg tggatgcaaa
    32101 ggatgagctc aaacgcgctc tcaaagaata caggggatct atccttatgg tctgccacga
    32161 gccagacttt tatgaaggtt ggatagacca aatatgggat tttaataatt taacttaaga
    32221 atgagagtaa aagaaataca gtaccgaatg tggatactgt atttttttgg tttttaagat
    32281 ttaaaatcgt agtctttcac cacttcgatg ctgtcgattg tgatagcagt agttggcttg
    32341 tctttttcat ctttttcggc tttagcaatc ttatccacaa catccatacc gtcaatcact
    32401 tgaccaaaga ctgggtgttt gccgtctaga ctagggtttc caccttcttt gtaggcttcg
    32461 ataattttct gtggatactt gcttgtaggg agtttagaag aggtatctgt agagttttgg
    32521 ttgatgaaga actggctgcc attggtgttt ggttgaccag tattagccat agcaagagca
    32581 ccacggatgt tatacaaata aggagtaatc tcgttcttga aaccagttcc tttgtcttta
    32641 gtcttatcct tgtcatgcca gatggactga ccacctgtac cgtccccttt tggatctcca
    32701 gtttggacca taaagccatc gatgacacgg tggaaggtaa taccgttata gtagccttct
    32761 ttggcgtgag tgaggaaatt ttcaaccgct agaggagcga gtttagggaa gagtttaatg
    32821 cgaatatcac cttggcttgt gtggagaatc acttcggctt catcttcagc aacttcctta
    32881 gatagttgtg ggaaattggc gttttcgttt gttaaagcat ggtttaactc cttggcagat
    32941 tgggcagcta ctttggaact ttcttcagca gcaagactgg aatcaacata atcatcacca
    33001 cgcagactgc gttggacgct gctacaccca gctagggcta cagtagacag taaaagaagg
    33061 gttgctagtt ttttcattct gttcctctca aaaattcatt tataccattg taccctaaaa
    33121 ggaagtggat ttcaaatatt agtagtttac agaactggct tagataatag ttgggatttc
    33181 atcatcatct tgaaaatctt caacttcttc cccagtaaat ggtttctgaa aagaacttag
    33241 tataaaacgg gcaattggtt caacaattag gaaatttaga ggaagagctg caataaagtt
    33301 tacgaaccag gcgcttagat aggtattggg tgtgatagca atgccattgt aaaaaagtcc
    33361 atagaaggac atgaaagtga ccagctaatt cttcttctga aaaatgctca tgaagggctt
    33421 gatccaggtc gcaagatttc attttagaaa gtccagtaat tcccttcttc ttcaggatat
    33481 tctttttcat ggtagtattt agatggtcaa gtgagtcaaa agaagtttcg acaagagcat
    33541 accctttttc taccagagtt tttagatgtt tagggggcat caataccata agtgtactca
    33601 aagtattttg gaaaccagat cttaagaaag ctcgtaaagc tagtcagttc tctaaacgct
    33661 aagaactagc tacaacacag catttacaac acttcatggt tcacaccatg gggtgttttt
    33721 taatgatttt aagcaaaatt cacacttaat tgaaagttat ttaaattaga tgaaaattta
    33781 ttttttgaaa aagtgggaaa acattgattc taagcgaatt ttgaatcata ttcgaatggg
    33841 agtttttaaa atcatgtttc agtggtaaaa gtaccaaact ggattctcca tagtaaaatg
    33901 atatctcctg ctagtgaatc atctgctaat aagtccatat ttcgcttggg aacgggcttg
    33961 ggacttagga gccaagtatc caaatctcgt tcaggcgata tgaagggttt gacttggtgt
    34021 tctttataaa atttttctaa aatagtattc acggatagca tctttcaaac atttatttcc
    34081 tattctaaca aatttctaaa tactttaaga aatttatagt ctgtcaagtt tttttcttga
    34141 cacatgccag aaatctgcta taatagaaca tgtgctaaat agctcagcta tttcaccgaa
    34201 ataaaaaatt aaagaaatga gactttaaaa atggcagtta aaatccgttt gactcgtatg
    34261 ggttctaaga aaaaaccttt ctaccgtatc aacgtagcag attcacgttc accacgtgac
    34321 ggacgtttca tcgaaacagt tggaacttac aacccacttg ttgctgaaaa ccaagtaact
    34381 ttgaaagaag accgcgttct tgcatggttg gctaatggag ctcaaccttc agacacagta
    34441 cgtaacatcc tttcaaaaga aggcgtattg aaaaaattcc acgattctaa attctcaaaa
    34501 taagtttaaa gtaggttgac agatggatac gattgaaaat ctcattattg cgattgtgaa
    34561 acccttaatt tcacaaccag atgccttaac tatcaagatt gaagatacac cagaattttt
    34621 ggaatatcat ttgaatcttg atcaaagcga tgtgggtcgt gtaatcggtc gtaagggtcg
    34681 cactatttct gcgataagaa cgattgtcta ctctgtccca actgaataca aaaaagtaag
    34741 aatcgttatt gacgaaaaat aagaagggcg ggacggatgt ctcgcttttt tgtgtggaat
    34801 agaagatata gtaaaatgaa ataataacag gacaaatcga tcaggacagt caaatcgatt
    34861 tctaacaatg ttttagaagt aaaggtgtac tattctagtt tcaatatact ataaaagatt
    34921 gaacgtttag gcaattccaa gactacttac tcgaacatta acagcagtct cggactgatg
    34981 aaggtctctt tatgaagtta gtggaggaag tcggagaagt agccgaggtt ttgaataggc
    35041 gctctggtcg aaaagaaggg ataggattcg aacgaggaac ctgccataga actggctgat
    35101 atcattcact acaccgtcgc tattgctgtt atcaagcata ttgacctaac tagaaccatc
    35161 tttgagaaag acaaaaccgc agccgttaat taccaacata aacatgattt ggaaggattc
    35221 ttgaagggaa agtagagatg ttgtcccctt cctccaactt gagaaaatct ttggaacgtg
    35281 ataaaataaa ggataaggat ttttaataat tcattatata gaatgagtgg atttctttat
    35341 gaagaggttt gaagtatcta cagaaattgg tagcctctct gttacttatc aaaaacaaaa
    35401 gaaagtgcta gtttgtctaa acggagcagg tttgctacca agttatgaaa atttttcact
    35461 tatacttgaa aaacctcctc ctacaattgg ttatttgaca attgattttc cgaacacagg
    35521 taggagtccg attcatgacc aagctggaaa aaatctagat aatcttgcag atgcggttta
    35581 tgaagtactt gaagaattag gaatttctga atatatactt tgtgcacata gttggagtgg
    35641 aattttagct tgcaaattac tcgagaaacc aattaagcgt cagactttag tagcaattga
    35701 accgacaact aaaaaagtca tgtttgctga tttttcagaa aatccttatc cagaaatgga
    35761 agagcagatg aggctgattg acgagtgtgg tcctgaactt tattttaaga acttaactca
    35821 agcaacattt agtcctgaaa cgaataaaaa aatctgggaa ttaatgcaag aaaaaggctt
    35881 agagttggaa aatcaagatc cagaatttca gatatctgga gagattactg aggaagattt
    35941 tgagaatttg tcgatagaat ctcatgtccc tgtatttatt ttttgtcaga cttatagaga
    36001 aaaagagtac agagaatcag aatattggac ttccaatact aaactcattt taggagggaa
    36061 tcaccattat ttacaatggt cagaatcgga aaaaattgcg gctattattc gagaattgtc
    36121 agaataagat ggaaaaaagg agattacagg agacaagatg aactacttta atgttgggaa
    36181 aatcgttaat acgcagggat tacagggtga gatgcgagtc ttgtctgtga cggattttgc
    36241 agaagaacgg tttaaaaaag gagctgagct ggctttgttt gatgaaaaag atcagtttgt
    36301 ccaaacagtg accatcgcta gccaccgtaa acagaagaac tttgacatta ttaaattcaa
    36361 agatatgtac catatcaata ctatcgaaaa gtacaaggga tacagtctca aggtcgctga
    36421 ggaagatttg aatgacctag acgatggtga attttactat cacgagatta tcggtttgga
    36481 agtctatgag ggtgatagct tggttggaac catcaaggaa atcctgcaac caggtgctaa
    36541 tgatgtctgg gtggtcaaac gaaaaggcaa acgtgatttg cttttacctt atatcccacc
    36601 agtggttctc aatgttgata ttccaaataa acgggtcgat gtggaaatct tagaagggtt
    36661 agacgatgaa gattgatatt ttaaccctct ttccagagat gttttctcca ctggagcact
    36721 caatcgttgg aaaggctcga gaaaaagggc tcttggatat ccagtatcat aattttcgag
    36781 aaaatgctga aaaggcccgt catgtagatg atgagcccta cggaggcggt cagggcatgt
    36841 tgctcagagc acaacctatt ttcgattcct ttgatgctat tgaaaagaaa aatccgcgcg
    36901 ttattctcct cgatcctgct ggaaagcagt ttgatcaggc ttatgctgaa gatttggctc
    36961 aagaggaaga gctaatcttt atctgtgggc actatgaggg ttatgatgag cgcattaaga
    37021 ccttggtaac agatgagatt tccctaggcg actatgtcct cactggtgga gaattggcag
    37081 ctatgaccat gattgatgct acagttcgcc tgattccaga agtgattggc aaggagtcta
    37141 gccaccaaga tgatagtttt tcttcaggtc ttttagaata tcctcagtac acacgtccct
    37201 atgattatcg aggcatggtc gtgccagatg tattgatgag tggccaccat gaaaagattc
    37261 gtcagtggcg attgtacgag agtttaaaga aaacctacga gcgcagacca gatttacttg
    37321 aacattatca actgacagta gaagaagaaa aaatgctggc agaaatcaaa gaaaacaaag
    37381 aataaaggag aaacctatgc aagtaatcaa acgtaatggc gaaattgctg aatttaatcc
    37441 agataagatt taccaagcca tcttgaaggc agcccaaact gtttatgttt tgacagatga
    37501 tttgcgtcaa aatcttgctc aagtcactaa gaaggtagtt ttggatttac aagaagccaa
    37561 ggtggaacgt gcgactatca gtatgattca atctatggtt gaacatcgtt tattgggcgc
    37621 aggttacatt accattgcag aacactatat ttcctatcgt ttacaacgtg acttggaaag
    37681 aagtggttat ggagatcata tcgcagttca tttacatttt gaacaaattc gctaagaaaa
    37741 agagtgggat gaagcaacta catctcactc tttcttaaat ctattttttt gggctgtttg
    37801 gacagttgag gggacaaatc gcaggcgttg aatatcgctt atacggataa tccggctcac
    37861 atttttggaa aaatttttca cgataatttg ctggccttgc tgatcatatt tgatgatatc
    37921 acctgtaaag cttgtctcag acaagataag gtgaacagcg gtttgtttct ggatagcctc
    37981 ttcaatagtg gctgtaaggg agttggtttc agtctggtca ggttggtcat gtccatttaa
    38041 aaaatcatgt attttatcta gaacttgctg gaatttatgt ctcatattgg cctcccttct
    38101 tttatatcaa tattatataa aattttccta aaatctacaa gattttacga ataaactaat
    38161 gaaactaaaa aaggagaaga aaatggcaga atttacattt gaaatcgaag agcacttgtt
    38221 gactctttct gaaaacgaaa aaggttggac caaggaaatt aaccgtgtga gctttaatgg
    38281 tgcccctgca aagtttgata ttcgtgcttg gagtccagac catactaaaa tgggcaaagg
    38341 gattactctc tcaaatgaag aatttcaaac gatggtggat gcctttaaag gcaactaata
    38401 ctcttcgaaa atctcttcaa accgcatcag ctttgccttg ccgtatgtat ggttactgac
    38461 ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag cagcctgcgg ctagcttcct
    38521 agtttgctct ttgattttca ttgagtataa gttttcaaaa taaaaaaaag gatattgagt
    38581 catgataact cggtatcctt ttttagttag taaagtaagt ctgattttta aggcgttgaa
    38641 gtaggggacg gctaataaaa ctaatagcca gaagtttgcc aaggtctgga atgataaagg
    38701 gaagaacccc cacaacaaga gctttttcaa atgccattcc agctaggaaa tgcaagctga
    38761 gaatcccgcc gacaaagaca agggcatcac ccaagaggtt tgcaagaaaa atcttaacaa
    38821 caccactctt gctgttggtt agagaggaag taagtccaga gtaaacgaga taaaaccaaa
    38881 gatagcctgc agtagggcca actaaagcct gaaaaccagc tccacctcct gcaaagacag
    38941 gaagaccgat agcacctaga agaagataga gtccagcaga aagtacagcc tctctcggtc
    39001 taaagacagt agcaatcaag ccgattgcaa agttttgcag agtgaaggga acaggtccaa
    39061 ttggaagact gatttgtgcc aaaacagcaa tgagagcagc cccaatagca gggatagcat
    39121 aaacgtgagc ttttttcaaa atagttaacc tcttttctaa tgtatagtaa ctattatact
    39181 catataggag tcgatgtcaa ccatttttta aaaacaaggt aacaaaaact gtaacagccg
    39241 attttcgtga ctagtcaagt tggataattt tttgttgatt cgaaaatcat taaaaaagaa
    39301 gcagacatat ttatatcagc ttcttttgtt tcgattaacg catggttaca aattcttcag
    39361 atgaagttgg gtgaatcgct acagttgcgt caaagtcagc cttggttgct cccattttga
    39421 tagcaacggc aaatccctga atcatttcat caacgccgta gccaattcca tgaagtccga
    39481 caactttttc ttctgaacca gctgttatga gtttgaaacg ggattcttga cggttgcgag
    39541 tgcaagcaga gtacatagat gcaaagcttg atttgtaaac cttgatttgg tcttgaccgt
    39601 attctttaat agcttgctct tctgtcaaac caacagttcc gatagcaggg tgtgaaaaga
    39661 caacagttgg aatagttgag taatccattt ttgcagtagt ttttccgtta aagagacgtt
    39721 cagatagggt acgtccggcc ttgattgcaa ctggagtcag ttctttctcg cccgttacat
    39781 cacctagagc atagattccc tcaacaacag tattttggta ttcatccact tggataaagc
    39841 cacgttcgtt cagagtcact ccagcttttt caagttgcaa gcccttaacg tttggacggc
    39901 ggcctgtagc ccagataact tggctagctg tgtgactagt accatcttcg aaatgaatgg
    39961 taatgccgtc agtagttttt tctaacttga cagggacttt gtgagtgtga agtggtaagt
    40021 ttgttctttc catttccttg accaaacctt caacgatgta ggaatcaaaa ccacgtaaag
    40081 gacgatcgcg gcgaacaaag agatctgtct tgacaccaaa agtgtggagt acgccagcta
    40141 attcaacggc gatataacca gcgcctagaa tggcaactga ctctggaagt tcttcccagg
    40201 caaatacatc atcagaagag ccacctagct cagcaccagg aatattagga atacttggat
    40261 gggcacctgt agcaatcacg atatgtttag cacgaatcag ttcaccattt acgcttacag
    40321 tatgagaatc tacaaattca gcatgacctt caatcaagtc tacaccgttg cgtttaaaac
    40381 taccatcata agaagaacga gcgcgatcaa tgtaggcttc acgattgcga cgtagggttg
    40441 caaagttaaa gttaagatca gtagtcttaa agccgtagtc ttctccaaat tgatggaaag
    40501 tctcagcgat ttgcgccccg taccacatga tttttttagg aacacaaccg acgttgacac
    40561 aggttccacc taatttcttt tcctcaataa cggctgcttt ggctccatgt tcaccagcac
    40621 ggttcatggt agcgattcct ccgctacctc caccgatagc aatgatatca tattctctca
    40681 ttgaaaactc ctttgtaatc ttttaaatag tagagtgtct tttttgatag ttatatttta
    40741 ttcttttttt gatttgattg caaaaatctg ctacgcacaa aaaattgcaa aaaagcttgc
    40801 caaataagtc aagatagtgt attatatatc tagtacaata gaaaagcaaa attccgaacg
    40861 attgtttaat cctgaactgc cattatttcc atctgaactg cattgtttgc tggagggaaa
    40921 tttggtataa tagcaaagta ttgtaaaaaa gaaaaggagt ttcattatga agaaaaagaa
    40981 tggtaaagct aaaaagtggc aactgtatgc agcaatcggt gctgcgagtg tagttgtatt
    41041 gggtgccggg gggattttac tctttagaca accttctcag actgctctaa aagatgagcc
    41101 tactcatctt gttgttgcca atgaaggaag cgtggcatcc tctgttttat tgtcagggac
    41161 agtaacagca aaaaatgaac aatatgttta ttttgatgct agtaagggtg atttagatga
    41221 aatccttgtt tctgtgggcg ataaggtcag cgaagggcag gctttagtca agtacagtag
    41281 ttcagaagcg caggcggcct atgattcagc tagtcgagca gtagctaagg cagatcgtca
    41341 tatcaatgaa ctcaatcaag cacgaaatga agccgcttca gctcaggctc cacagttacc
    41401 agcgccagta ggaggagaag atgcaacggt gcaaagccca actccagtgg ctggaaattc
    41461 tgttgcttct attgatgctc aattgggtga tgcccgtgat gcgcgtgcag atgctgcagc
    41521 gcaattaagc aaggctcaaa gtcaattgga tgcaacaact gttctcagta ccctagaggg
    41581 aactgtagtc gaagtcaata gcaatgtttc taaatctcca acaggggcga gtcaagttat
    41641 ggttcatatt gtcagcaatg aaaatttaca agtcaaggga gaattgtctg agtacaatct
    41701 agccaacctt tctgtaggtc aagaagtaag ctttacttct aaagtgtatc ctgataaaaa
    41761 atggactggg aaattaagct atatttctga ctatcctaaa aacaatggtg aagcagctag
    41821 tccagcagcc gggaataata caggttctaa atacccttat actattgatg tgacaggcga
    41881 ggttggtgat ttgaaacaag gtttttctgt caacattgag gttaaaagca aaactaaggc
    41941 tattcttgtt cctgttagca gtctagtaat ggatgatagt aaaaattatg tctggattgt
    42001 ggatgaacaa caaaaggcta aaaaagttga ggtttcattg ggaaatgctg acgcagaaaa
    42061 tcaagaaatc acttctggtt taacgaacgg tgctaaggtc atcagtaatc caacatcttc
    42121 cttggaagaa ggaaaagagg tgaaggctga tgaagcaact aattagtcta aaaaatatct
    42181 tcagaagtta ccgtaatggt gaccaagaac tgcaggttct caaaaatatc aatctagaag
    42241 tgaatgaggg tgaatttgta gccatcatgg gaccatctgg gtctggtaag tccactctga
    42301 tgaatacgat tggcatgttg gatacaccaa ccagtggaga atattatctt gaaggtcaag
    42361 aagtggctgg gcttggtgaa aaacaactag ctaaggtccg taaccaacaa atcggttttg
    42421 tctttcagca gttctttctt ctatcgaagc tcaatgctct gcaaaatgta gaattgccct
    42481 tgatttacgc aggagtttcg tcttcaaaac gtcgcaagtt ggctgaggaa tatttagaca
    42541 aggttgaatt gacagaacgt agtcaccatt taccttcaga attatctggt ggtcaaaagc
    42601 aacgtgtagc cattgcgcgt gccttggtaa acaatccttc tattatccta gcggatgaac
    42661 cgacaggagc cttggatacc aaaacaggta accaaattat gcaattattg gttgatttga
    42721 ataaagaagg aaaaaccatt atcatggtaa cgcatgagcc tgagattgct gcctatgcca
    42781 aacgtcagat tgtcattcgg gatggggtca tttcgtctga cagtgctcag ttaggaaagg
    42841 aggaaaacta agatgcagaa tctgaaattt gccttttcat ctatcatggc tcacaagatg
    42901 cgttctttgc ttactatgat tgggattatt atcggtgttt catcagttgt tgtgattatg
    42961 gctttgggtg attccctatc tcgtcaagtc aataaagata tgactaaatc tcagaaaaat
    43021 attagcgtct ttttctctcc taaaaaaagt aaagatgggt cttttactca gaaacaatca
    43081 gcttttacgg tttctggaaa ggaagaggaa gttcctgttg aaccgccaaa accgcaagaa
    43141 tcctgggtcc aagaggcagc taaactgaag ggagtggata gttactatgt aaccaattca
    43201 acgaatgcca tcttgaccta tcaagataaa aaggttgaga atgctaattt gacaggtgga
    43261 aacagaactt acatggacgc tgttaagaat gaaattattg caggtcgtag tctgagagag
    43321 caagatttca aagagtttgc aagtgtcatt ttgctagatg aggaattgtc cattagttta
    43381 tttgaatctc ctcaagaggc tattaacaag gttgtagaag tcaatggatt tagttaccgg
    43441 gtcattgggg tttatactag tccggaggct aaaagatcaa aaatatatgg gtttggtggc
    43501 ttgcctatta ctaccaatat ctcccttgct gcgaatttta atgtagatga aatagctaat
    43561 attgtctttc gagtgaatga taccagttta accccaactc tgggtccaga actggcacga
    43621 aaaatgacag agcttgcagg cttacaacag ggagaatacc aggtggcaga tgagtccgtt
    43681 gtatttgcag aaattcaaca atcgtttagt tttatgacga cgattattag ttccatcgca
    43741 gggatttctc tctttgttgg aggaactggt gtcatgaaca tcatgctggt ttcggtgaca
    43801 gagcgcactc gtgagattgg tcttcgtaag gctttgggtg caacacgtgc caatatttta
    43861 attcagtttt tgattgaatc catgattttg accttgttag gtggcttaat tggcttgaca
    43921 attgcaagtg gtttaactgc cttagcaggt ttgttactgc aaggtttaat agaaggtata
    43981 gaagttggag tatcaatccc agttgcccta tttagtcttg cagtttcggc tagtgttggt
    44041 atgatttttg gagtcttgcc agccaacaag gcatcgaaac ttgatccaat tgaagccctt
    44101 cgttatgaat gagatcaaca agatggacac tcgtctatct tgtttttgta tggatttccc
    44161 tatcgaaatt cattaaaaat gtgatataat gaagtgttag aaaaacaggt ttcatttgcc
    44221 tggaaaggaa aaattatgtc tgaaaaaaat ttttatatta caacgccgat ttactatcca
    44281 tctgggaaac ttcatatcgg ttctgcctac acaactatcg catgtgatgt cctagcacgt
    44341 tacaaacgcc tgatgggcta cgatgtattt tatctgacag gtcttgatga acatggtcag
    44401 aaaatccagc agaaagcgga agaagctggt attacacctc aagcctatgt tgatggaatg
    44461 gcggttggag ttaaagaact ctggcaatta ctagatatct catacgataa atttatccgt
    44521 acaactgatg actaccatga aaaagttgtc gcacaggtct ttgaacgctt acttgctcaa
    44581 gatgatattt acttgggtga atactctggt tggtattcag tatcagacga agaattcttt
    44641 acagaaagcc agctggcaga agttttccgt gatgaagctg gaaatgtgac tggtggtatt
    44701 gctccatcag gtcatgaggt tgaatgggtt tctgaagaat catactttct tcgccttagc
    44761 aaataccaag accgtttggt cgaatttttc aaagctcatc ctgaatttat cacgccagat
    44821 ggtcgcctta atgaaatgct acgcaacttc atcgagccag gtttggaaga tttggcggta
    44881 tctcgtacaa cctttacatg gggagtgcct gtcccatcaa atccaaaaca cgttgtctac
    44941 gtttggattg atgcccttct taactatgcg acagctcttg gctacgctca agacgaacat
    45001 ggtaactttg acaagttctg gaatggaaca gtcttccata tggtaggaaa agatatcctt
    45061 cgcttccact ctatctactg gccaatcctt cttatgatgt tggatgttaa attacctgat
    45121 cgtttgattg cccatggttg gtttgtcatg aaagacggaa aaatgtctaa gtcaaaaggg
    45181 aatgtcgttt accctgaaat gttggtagag cgttatggac tagatccact tcgttactac
    45241 ctcatgcgta accttccagt tggttcagac ggaaccttta ctcctgaaga ctatgttggc
    45301 cgtatcaact atgaattggc taatgacctt gggaacctcc ttaaccgtac ggtttccatg
    45361 attaataagt actttgatgg acaaatccct gcctatgtag aaggtgtgac tgaatttgat
    45421 catgttcttg ctgaggttgc agaacaatca atcgcagact tccatacaca catggaagca
    45481 gttgactatc cacgtgcgct tgaagcagtc tggactctga tctctcgtac caataaatac
    45541 atcgatgaga ccgcaccatg ggtcttggcc aaggatgaag ctcttcgtga ccaattggca
    45601 agtgtcatga gccacttggc agccagcatt cgtgtagttg ctcacttgat tgaaccattt
    45661 atgatggaaa ctagtcgtgc agttttgact caacttggtt tggaagaagt ttctagtctt
    45721 gaaaacttga gtttggctga cttcccagca gatgtgactg tagttgccaa aggaacacct
    45781 atctttccac gtctaaatat ggaagaagaa atcgcctata tcaaggaaca aatggaaggc
    45841 aataaaccag cagtcgaaaa agaatggaat ccggacgaag ttgagctcaa actaaacaag
    45901 gatgaaatca agtttgaaga ctttgacaag gttgaaatcc gtgtggcaga agtcaaagaa
    45961 gtgtctaaag tagaaggttc agataagttg cttcaattct gcttggatgc tggtgatgga
    46021 gaagatcgtc agattctttc aggaattgcc aaatactatc caaatgaaca agaattggtc
    46081 ggcaagaaag ttcaaatcgt tgctaacctc aaaccacgta aaatgatgaa aaaatatgtc
    46141 agccagggta tgattctctc agctgaacat gatggcaaat taacccttct cacagttgat
    46201 ccagctgtac caaatggaag tgtgattggg taaaagtaga caggactagt tcaggctagt
    46261 tctgtttttt tatttatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    46321 ttgctcaaaa cac
//
LOCUS       DBERCA010000011        45623 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.011, whole genome shotgun sequence.
ACCESSION   DBERCA010000011 DBERCA010000000
VERSION     DBERCA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.011"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            476..1762
                     /locus_tag="ACL719_000701"
     CDS             476..1762
                     /locus_tag="ACL719_000701"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGS0698711.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYISTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1777..2088
                     /locus_tag="ACL719_000702"
     CDS             1777..2088
                     /locus_tag="ACL719_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGS0698712.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2334..3062
                     /locus_tag="ACL719_000703"
                     /pseudo
     CDS             2334..3062
                     /locus_tag="ACL719_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            3397..4288
                     /locus_tag="ACL719_000704"
                     /pseudo
     CDS             3397..4288
                     /locus_tag="ACL719_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4304..5032
                     /locus_tag="ACL719_000705"
     CDS             4304..5032
                     /locus_tag="ACL719_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698713.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLGLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            5418..5627
                     /locus_tag="ACL719_000706"
     CDS             5418..5627
                     /locus_tag="ACL719_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0698714.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            5617..6105
                     /locus_tag="ACL719_000707"
     CDS             5617..6105
                     /locus_tag="ACL719_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGS0698715.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            6221..7681
                     /locus_tag="ACL719_000708"
     CDS             6221..7681
                     /locus_tag="ACL719_000708"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGS0698716.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGAQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            7678..8502
                     /gene="nadE"
                     /locus_tag="ACL719_000709"
     CDS             7678..8502
                     /gene="nadE"
                     /locus_tag="ACL719_000709"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGS0698717.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            8852..9403
                     /locus_tag="ACL719_000710"
     CDS             8852..9403
                     /locus_tag="ACL719_000710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0698718.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWA
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            10448..10675
                     /locus_tag="ACL719_000711"
     CDS             10448..10675
                     /locus_tag="ACL719_000711"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS0698719.1"
                     /translation="MKVPAESKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVT
                     TITYNVDGKSGQVTESTLSQKKDSQTRVVKK"
     gene            10864..11253
                     /locus_tag="ACL719_000712"
     CDS             10864..11253
                     /locus_tag="ACL719_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS0698720.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(11914..12942)
                     /gene="queA"
                     /locus_tag="ACL719_000713"
     CDS             complement(11914..12942)
                     /gene="queA"
                     /locus_tag="ACL719_000713"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGS0698721.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            13093..13800
                     /locus_tag="ACL719_000714"
     CDS             13093..13800
                     /locus_tag="ACL719_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGS0698722.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            13940..14116
                     /gene="rpsU"
                     /locus_tag="ACL719_000715"
     CDS             13940..14116
                     /gene="rpsU"
                     /locus_tag="ACL719_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGS0698723.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            14246..15181
                     /gene="hprK"
                     /locus_tag="ACL719_000716"
     CDS             14246..15181
                     /gene="hprK"
                     /locus_tag="ACL719_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGS0698724.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            15174..15962
                     /gene="lgt"
                     /locus_tag="ACL719_000717"
     CDS             15174..15962
                     /gene="lgt"
                     /locus_tag="ACL719_000717"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGS0698725.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            15963..16389
                     /locus_tag="ACL719_000718"
                     /pseudo
     CDS             15963..16389
                     /locus_tag="ACL719_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            16362..16751
                     /locus_tag="ACL719_000719"
     CDS             16362..16751
                     /locus_tag="ACL719_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGS0698726.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            16835..17965
                     /gene="hemW"
                     /locus_tag="ACL719_000720"
     CDS             16835..17965
                     /gene="hemW"
                     /locus_tag="ACL719_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGS0698727.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLNAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            17970..18707
                     /locus_tag="ACL719_000721"
     CDS             17970..18707
                     /locus_tag="ACL719_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGS0698728.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            18718..19491
                     /locus_tag="ACL719_000722"
     CDS             18718..19491
                     /locus_tag="ACL719_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGS0698729.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            19481..20098
                     /locus_tag="ACL719_000723"
     CDS             19481..20098
                     /locus_tag="ACL719_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGS0698730.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            20310..20711
                     /locus_tag="ACL719_000724"
     CDS             20310..20711
                     /locus_tag="ACL719_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGS0698731.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            20711..20989
                     /locus_tag="ACL719_000725"
     CDS             20711..20989
                     /locus_tag="ACL719_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGS0698732.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            20979..21752
                     /locus_tag="ACL719_000726"
     CDS             20979..21752
                     /locus_tag="ACL719_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGS0698733.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            21753..23057
                     /locus_tag="ACL719_000727"
     CDS             21753..23057
                     /locus_tag="ACL719_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGS0698734.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            23631..24509
                     /gene="pstS"
                     /locus_tag="ACL719_000728"
     CDS             23631..24509
                     /gene="pstS"
                     /locus_tag="ACL719_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGS0698735.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            24509..25426
                     /gene="pstC"
                     /locus_tag="ACL719_000729"
     CDS             24509..25426
                     /gene="pstC"
                     /locus_tag="ACL719_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS0698736.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            25416..26300
                     /gene="pstA"
                     /locus_tag="ACL719_000730"
     CDS             25416..26300
                     /gene="pstA"
                     /locus_tag="ACL719_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS0698737.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            26311..27114
                     /gene="pstB"
                     /locus_tag="ACL719_000731"
     CDS             26311..27114
                     /gene="pstB"
                     /locus_tag="ACL719_000731"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0698738.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            27127..27885
                     /gene="pstB"
                     /locus_tag="ACL719_000732"
     CDS             27127..27885
                     /gene="pstB"
                     /locus_tag="ACL719_000732"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0698739.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            27897..28550
                     /gene="phoU"
                     /locus_tag="ACL719_000733"
     CDS             27897..28550
                     /gene="phoU"
                     /locus_tag="ACL719_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS0698740.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            28707..29522
                     /locus_tag="ACL719_000734"
     CDS             28707..29522
                     /locus_tag="ACL719_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0698741.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(29542..30813)
                     /locus_tag="ACL719_000735"
     CDS             complement(29542..30813)
                     /locus_tag="ACL719_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGS0698742.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            30955..31686
                     /gene="budA"
                     /locus_tag="ACL719_000736"
     CDS             30955..31686
                     /gene="budA"
                     /locus_tag="ACL719_000736"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGS0698743.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            31696..32449
                     /locus_tag="ACL719_000737"
                     /pseudo
     CDS             31696..32449
                     /locus_tag="ACL719_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            32561..33511
                     /gene="murB"
                     /locus_tag="ACL719_000738"
     CDS             32561..33511
                     /gene="murB"
                     /locus_tag="ACL719_000738"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGS0698744.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            33570..34727
                     /locus_tag="ACL719_000739"
     CDS             33570..34727
                     /locus_tag="ACL719_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698745.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            34708..35514
                     /locus_tag="ACL719_000740"
     CDS             34708..35514
                     /locus_tag="ACL719_000740"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698746.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            35511..36284
                     /locus_tag="ACL719_000741"
     CDS             35511..36284
                     /locus_tag="ACL719_000741"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698747.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            36281..37351
                     /locus_tag="ACL719_000742"
     CDS             36281..37351
                     /locus_tag="ACL719_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0698748.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            37612..37851
                     /locus_tag="ACL719_000743"
     CDS             37612..37851
                     /locus_tag="ACL719_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698749.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            38126..38611
                     /locus_tag="ACL719_000744"
     CDS             38126..38611
                     /locus_tag="ACL719_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGS0698750.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            38633..41251
                     /gene="alaS"
                     /locus_tag="ACL719_000745"
     CDS             38633..41251
                     /gene="alaS"
                     /locus_tag="ACL719_000745"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGS0698751.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GNIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQLSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGR
                     NQAKIQELLDAVAGKL"
     gene            complement(41341..42795)
                     /locus_tag="ACL719_000746"
     CDS             complement(41341..42795)
                     /locus_tag="ACL719_000746"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGS0698752.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFIDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            42974..43723
                     /locus_tag="ACL719_000747"
     CDS             42974..43723
                     /locus_tag="ACL719_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698753.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCNRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            43704..45338
                     /locus_tag="ACL719_000748"
     CDS             43704..45338
                     /locus_tag="ACL719_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698754.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYRSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT        13254 a         8427 c        10213 g        13729 t
ORIGIN      
        1 ttctttgagc ttttttcttt ttaaatattg attgtttcta aaagttctag taatattgct
       61 atttctcatt tttccaaata tgttattcaa cttattagct acgtctctat tcatatttgg
      121 gtataaaaca aaaaatcaat tctaacagtt ttatatcaga ataataaata aaaaagcgat
      181 attctagact gtatcaatcc ttttccatta taggagcaac agagtgggag tagtcatcta
      241 aggactaatt tatgtatttt tacgagtcag tatctttgga tactggtttt tacttttcta
      301 gactttttga ctacttgtta aaactgggat aattttcgac tgtttaacag ttattatgca
      361 aagtctaaaa gattagaatt gtcaaaacaa tccgtctagg cttgatttta tcctttattt
      421 actataaaat gagaaggaaa aatgtcaaac ttttatattg caaataggag aaattatgac
      481 aaaaacatta aaacgtcctg aggttttatc acctgcaggg actttagaga agctaaaggt
      541 agctgttcag tatggagcag atgctgtctt tatcggtggt caggcctatg gtcttcgtag
      601 ccgtgccgga aactttactt tcgaacagat ggaagaaggc gtgcagtttg cggccaagta
      661 tggtgccaag gtctatgtag cggctaatat ggttatgcac gaaggaaatg aagctggtgc
      721 tggtgagtgg ttccgtaaac tgcgtgatat cgggattgca gcagttatcg tatctgaccc
      781 agccttgatt atgattgcag ctactgaagc accaggcctt gaaatccacc tttctaccca
      841 agccagtgcc actaactatg aaacccttga gttctggaaa gagctaggct tgactcgtgt
      901 cgttttagcg cgtgaggttt caatggaaga attagctgaa atccgcaaac gtacagatgt
      961 tgaaattgaa gcctttgtcc atggagctat gtgtatttca tactctggac gttgtactct
     1021 ttcaaaccac atgagtatgc gtgatgccaa ccgtggtgga tgttctcagt catgccgttg
     1081 gaaatacgac ctttacgata tgccatttgg gaaagaacgt aagagtttgc agggtgagat
     1141 tccagaagaa ttttcaatgt cagccgttga catgtctatg attgaccaca ttccagatat
     1201 gattgaaaat ggtgtggaca gtctaaaaat cgaaggacgt atgaagtcta ttcactacat
     1261 atcaacagta accaactgct acaaggcggc tgtggatgcc tatcttgaaa gtcctgaaaa
     1321 gtttgaagct atcaaacaag acttggtgga cgagatgtgg aaggttgccc aacgtgaatt
     1381 ggcaacaggt ttctactacg gtataccatc tgaaaatgag cagttgtttg gtgctcgccg
     1441 taaaattcct gagtacaagt ttgtcgctga agtggtttct tatgatgatg cggtacaaac
     1501 agcaactatt cgtcaacgaa acgtcattaa cgaaggggac caagttgagt tttatggtcc
     1561 aggtttccgt cattttgaaa cctatattga agatttgcat gatgccaaag gcaataaaat
     1621 cgaccgcgct ccaaatccaa tggaactatt gactattaaa gtcccacaac ccgttcaatc
     1681 aggagacatg gttcgtgcat taaaagaagg actcatcaat ttttataagg aagatggaac
     1741 cagcgtcaca gttcgagctt aagaaaggaa aaggaaatga tagaggcaca gggtttctta
     1801 gtggataagc aaacaagatg cattcattac catagcaagc tggatattat tgctttacaa
     1861 tgctatgatt gtaaaaagta ttatgcttgt tatcggtgtc atgattcatt agaaaatcac
     1921 ccttttgagc cgtatccctt atctttgata caggataagc ctattttatg tggtgtttgt
     1981 ctaaaactac taacatataa gcaatataaa gaaagtttaa gttgcccctt ttgtttttct
     2041 cgctttaatc caggttgcca aaatcataag gaacgctatt ttaaatagca aatcatctag
     2101 ttttgaagta ggagaaaact caattccaag agaaaatgaa gtaaatcttc ccacaataaa
     2161 acgcataata tcaagattgt tcaatacctg atactatgcg tttttaagat tttaaagact
     2221 tttttccttt atctggtatt ttgactactt gttaaaactg ggttaatttt caactgttta
     2281 acagttatta tgcaaagtct aaaagattag aattgtcaaa acaatccgtc tagagtatgc
     2341 gtgatgccaa ccgtggtgga tgttctcagt catgccgttg gaagtacgac ctttacgata
     2401 tgccatttgg gaaagaacgt aagagtttgc agggtgagat tccagaagaa ttttcaatgt
     2461 cagccgttga catgtctatg attgaccaca ttccagatat gattgaaaat ggtgtggaca
     2521 gtctaaaaat cgaaggacgt atgaagtcta ttcactacat atcaacagta accaactgct
     2581 acaaggcggc tgtggatgcc tatcttgaaa gtcctgaaaa gtttgaagct atcaaacaag
     2641 acttggtgga cgagatgtgg aaggttgccc aacgtgaact ggctacagga ttttactatg
     2701 gtacaccatc tgaaaatgag cagttgtttg gtgctcgtcg taaaattcct gagtacaagt
     2761 ttgtcgctga agtggtttct tatgatgatg cggcacaaac agcaactatt cgtcaacgaa
     2821 atgtcattaa cgaaggggac caagttgagt tttatggtcc aggtttccgt cattttgaaa
     2881 cctatattga agatttgcat gatgccaaag gcaataaaat cgaccgcgct ccaaatccaa
     2941 tggaactatt gactattaaa gtcccacaac ctgttcaagc tggagacatg gttcgagctc
     3001 ttaaagaggg gcttatcaat ctttataagg aagatggaac cagcgtcaca gttcgtgctt
     3061 aatgtagttg tttagtttta aaaaattatg caaagctcca tatacaacac ttaaacgaga
     3121 ttaaagaatg gcgaaatccc ttgatgcgca agggattagc tgtctttttt attttttaag
     3181 tgataaagtc ggagcttagg tatcaaagcc tatcaaatta aacaaagaag cgatgtctta
     3241 gatattttga aaaaaattaa taagcagaaa actctctatt attttgttgt agagagtttt
     3301 ttgttaataa aattttacaa aatgacattt atatattgca ttaagttaga tatatgatat
     3361 aatgttgtta aaaagaggcg caacttttta aactcaataa gaatcaaaga gcaaaccgag
     3421 aatattaatg gaggaataaa aaatgtaagt tataaatgta agtaagcatt atggtcattc
     3481 aatcattctc aaagatataa attttgcact taacaagggt gaaattgttg gtctagtagg
     3541 gagaaacgga gttggtaaga gtacattgat gaaaattctt gttcagaata atcaaccgac
     3601 ttcagggaat attataagta gtgataatgt tgggtattta attgaagaac caaaattatt
     3661 tttatctaaa acaggtttag agaatttaaa atatctggca aacttatatg gtgttgacta
     3721 caatcaagaa agatttagaa gtttgatcca agagttagat ttgactcagt ctattaataa
     3781 aaaagtaaag acctattctt tgggtacaaa acaaaaatta gctttgcttc taactctcgt
     3841 tacgaaacct gatatattga ttttagatga accgactaat ggtttagata ttgaatcatc
     3901 acaaatagtt ttagcggttc taaaaaaatt agctttaaat gaaaacgtgg gaattttaat
     3961 atcgagtcat aaattagaag acattgaaga aatttgtgag agagttcttt tcttggagaa
     4021 cgggcttttg acatttcaaa aagtaggaaa agatagtcat aatttcttgt ttgagatagc
     4081 tttttcatca gctacagata gagacatttt cattaccaaa caagaatttg gggatattgt
     4141 tcaggaagag ggattgagaa ttactatgtc tgggaatatt caaagtagtg agctttttaa
     4201 attttttaac gaaaactcta ttaaagtagt tgattttgaa actaaaaaag agacgcttaa
     4261 agatatttac ctaaatcgtt caaaataaag gaaggttata atcatgaaat taaataaatt
     4321 gaattttctt aaggaaaata taagaaattt atattcatca ggcgtaatat atctcggttt
     4381 gcttatctcg tttataccgc cgatattgct tacattcttt attctaaaga ctcaagggac
     4441 atcgcttggt attaagcata tttcaaactt ttatgctatg ctcggtatgt taatggctgt
     4501 tatacatgct aatcgagtca ttagtagaga tttttcccac aatacggtaa gtttgtttta
     4561 taatcaacag aagaatcgga tgatttacgt cttgtctaat tttctatatg ccatctcagt
     4621 ttccattatt tatgctttga atggcattgt gttactagtc atagtaagta aattgggtat
     4681 tccaggtgat ttaggattag attttatagt agctattgta gtcaatacaa ttttgttagt
     4741 cctgttttat tttctattat cttacatttt ctatttatac aaattgaaaa gtggcttggt
     4801 atttggtatt ttagtagctt tactactctt tatccctaat atattaaata cgatgatgat
     4861 gaatactagt aatgatttgt ttatcaaagc aattgaactt cttccttttt attctttacc
     4921 tgtatttgtg gcttcaaata cgatgtctat tagtcagtat cttgtggtaa tcactacaat
     4981 cattttattg tactttttca ctctcaagaa aagcaagaag tattcatttt agtttcattt
     5041 agtattattt tgcataccta aaatacaata aaaaaatcag tcatcttggt atgctcctgc
     5101 tttcactatt caacacgttt ttgacttata ctaggctcat ttccaaaagc attatataat
     5161 agtgatatga aaccaactaa actaaacaag aaatataagc aataaaaact cgtttaaaag
     5221 attctactaa agctaatact aaataaaaat aaaagagtaa actaggaagt ttatttcaaa
     5281 caacctaaaa tactgatttt cggctgaaga taatactgga gtgcaaatta atggggttat
     5341 aataaatagc tgatagcttg tgttggtttt ggatttttta agagtagatg agtattaaaa
     5401 ctataaggag gacgaaggtg gctaaaaatt taaaattaaa attagctcgg gtagagcgtg
     5461 atttaacaca aggtcaactg gcagaggctg tcggggtgac acgccagact attggtttga
     5521 tagaggcggg aaaatacaat cccagtctct cgctctgcca gtctatttgc agatgtttag
     5581 ggaaaaccct agaccaacta ttttgggagg aagaagatga aaaatagatt ttattattat
     5641 caattactag acgaaagaga agaacaactg ttcaataaag cgggatctga aagtttctat
     5701 atctgcattg ctttgtcgct cctatcttat atcatttcag tattagcacc aagccttttt
     5761 aattctaata tgctgctaat cgttatcatc atagggacat tttacttttt caatcgcgcc
     5821 cgttatctgg gagtgaccta ctatagtcgt tttcatttta cgattttggg ttgttttttc
     5881 ctaaccttgg ctattacggc tcttttgatg ttgcagaatt atcaattcaa catagaaatt
     5941 tatcagcaca atcctttgaa ttttaaatac ctgtctgctt gggtcattac ttatgtcatt
     6001 taccttccgt gggtctttat tggcaatctt ggtcttaaga gctatggcga atgggctcag
     6061 aaaaaatttg aacaagatat ggatgaattg gagagtggag aatagcttgt tactcttttc
     6121 tcaatccagc taaaatgtga tataatagta ctaatttatt ggaatacatg aaagttcttg
     6181 aaaattttca tgtgtttcta gctaaggaag taggaaaagt atgtatccag atgatagttt
     6241 gacattgcac acggacttgt accagatcaa catgatgcag gtttactttg accaagggat
     6301 tcacaataag aaggcggtct ttgaggtgta tttccgccaa cagcctttta agaacggcta
     6361 tgcggttttt gcaggtttgg aaagaattgt gaactatctt gaagacttgc gtttttcaga
     6421 tagtgatata gcctatttgg agtcgcttgg ttatcatggg gcgttcttgg attaccttcg
     6481 caatttcaag ttggagttga ccgttcgttc tgcccaagaa ggggatttgg tttttgctaa
     6541 tgaaccgatt gtgcaggtgg aaggacctct agcccaatgt cagttggtcg aaacggctct
     6601 tttgaacatc gtcaactacc agaccttggt ggcgacgaag gcagctcgta ttcgttcggt
     6661 tatcgaagat gaacccttga tggagtttgg gacacgtcgg gctcaagaaa tggatgcggc
     6721 catctgggga acacgcgcag cggtgattgg tggggccaat ggaaccagca acgtgcgtgc
     6781 gggtaagctc tttgacattc ctgttttggg aacccatgcc catgccttgg tacaggttta
     6841 tggcaatgac tatgaagctt tcaaggctta cgctgcgacc cacaaaaatt gtgtctttct
     6901 tgtggacacc tatgacaccc ttcgcatcgg tgtaccagct gccattcagg tggcgcgtga
     6961 gctgggtgct cagattaact ttatgggtgt gcggattgac tctggggata ttgcctacat
     7021 ttctaagaaa gtccgtcagc aactggacga ggctggattt acagaggcta agatttatgc
     7081 ttctaatgat ttggacgaaa atactatcct caatctcaag atgcaaaagg ccaagattga
     7141 tgtctggggc gtgggtacca agctgattac agcttatgac cagccagctc ttggtgcagt
     7201 ttacaagatt gttgcaatcg aagatgaaac tggtcagatg cgcaatacga ttaagctgtc
     7261 taataatgct gaaaaagttt ctacgccagg taagaagcag gtgtggcgca ttaccagtcg
     7321 tgaaaaaggc aagtcagaag gtgactacat cacttatgat ggtgtggata ttagcgacat
     7381 gacagaaatc aagatgttcc atccgaccta tacatacatc aagaagacgg ttcgtaattt
     7441 tgatgccgtt cctctcttgg tggatatctt caaagaagga atattagttt acaacttgcc
     7501 tagtttgact gacattcagg attatgcccg taaggaattt gacaagttgt gggatgagta
     7561 taagcgtgtg ctcaatccgc agcactatcc agtggatttg gcgcgtgacg tatggcaaga
     7621 taagatggac ttgattgata agatgcgcaa ggaagccctt ggtgaaggag aagaagaatg
     7681 agtttgcaag aaacgattat ccaagagctg ggtgtcaaac cagtgattga tgcccaggaa
     7741 gaaatccgtc gttctattga tttcttaaaa agatatctga aaaaacatcc cttcctaaaa
     7801 acctttgtac tagggatttc tgggggacaa gactcaacct tggcaggacg tttggcgcaa
     7861 ttagctatgg aagaactgcg agctgaaacg ggagacgata gctacaaatt tatcgctgtc
     7921 cgcctgccat acggagtgca agctgatgaa gcagatgctc aaaaagccct agccttcatc
     7981 cagccagatg tcagcttggt tgtgaatatc aaggaatcag ctgatgccat gacagctgca
     8041 gttgaagcga caggtagtcc tgtttcagac ttcaacaagg ggaatatcaa ggcacgttgc
     8101 cgtatgattg ctcagtatgc ccttgctggt tcccatagcg gagcggtcat tggaacagac
     8161 cacgccgcag aaaatatcac aggtttcttt accaagtttg gtgacggcgg tgcggatatt
     8221 ctccctcttt accgcctcaa taaacgccaa ggaaaacagc tcttgcagaa acttggcgca
     8281 gagccagccc tttatgaaaa aatcccaacg gcagacctag aagaagataa accaggccta
     8341 gctgacgaag tcgcacttgg agtcacctac gcagagattg acgactacct agaaggcaaa
     8401 acaatcagcc cagaagctca agcgaccatt gaaaactggt ggcacaaagg ccaacacaaa
     8461 cgccacttac ccatcaccgt atttgatgac ttttgggagt aaaaaggtcc gggggacctt
     8521 tttagcttct tgccctgaaa ttaaaaagca agaaaaacct ccactggagg ttttcagcct
     8581 ctcatcttga aataagaaag tgagagaagg tccgggggat cttgaacctc gagtttagaa
     8641 ataagaaaat gaggcagatt cggtaactcg aagagttcga tttcatcgtc ttacccctgc
     8701 aacgatgact aggtttgaaa aagcttgcta gatcgcattt caaaccaggc agcgactgcg
     8761 tcaagaaatt agaagacaaa ctcgttttct agctgttact gagttgagcc tttttactac
     8821 gagcatagaa ataaggaagt gaggtagcat catgaaatct attggtacgc aaatattaca
     8881 gacagaacgt ttgattttaa gaagatttgt ggagagtgat gcagaagcca tgtttcaaaa
     8941 ttgggcttca tccgctgaga atctgaccta tgttacctgg gctccccatc ctgatgtcga
     9001 aatcactcga aactcgattc gcaattgggt tgcttcctat actaatctca actattacaa
     9061 atgggccatt tgtctaaaag aaaacccaga gcaagtgata ggagatatca gcatcgttaa
     9121 gatagacgag gctgatttaa gctgtgaaat cggctatgtg ctaggcaagg cttactgggg
     9181 acatggtatg atgacagagg ccttgaaagc tgtcttggac ttttgtttta ctcaagcagg
     9241 ttttcaaaag gtcagagcac gttatgccag tctcaaccca gcttcaggtc gtgtcatgga
     9301 aaaggctgga atgtcctatc tacaaaccat tgttaatggt gtagagagaa aaggctatct
     9361 tgcggatctt atttattatg gtataagtag ggaagaatgt tgaattctat tttctgtttc
     9421 tatcgaagtc aactatttat tgtaaatata ataattagca ttccaagttt atttgcaatt
     9481 ttaaaatagc atattgatta gtacaagaca gatgttctag ttccttcttt aatctggttt
     9541 agtattagtt aaaaaatcgc tttaagcttg taactaagag ggagctaatc gactagattc
     9601 tccagccgaa caggtggtaa tgtacttttt atagtgtaat cctagctgtt gttaaattta
     9661 aaatagaatc ctctatcgag ttagggaatt aaattcaacc aattttattt atgttttttc
     9721 tatcaaatta tctaatatta aaatagtctc attctgatga gaaaactatt ctatttatac
     9781 tgcatcgttg tgtaaggaat gatgaaagat gagtgaaaat tatcaagttg gaatgtttgt
     9841 atctaaatat attagcatgt atttagatca gatgtctgca atcctttata tatgagattg
     9901 attacgatat agttaaagtg ttagttcaat agtaaatata tcattctaag tctattttat
     9961 atataaagta tattgtttaa cgtttgatag aaaagatgct atactagaag taggatgtaa
    10021 tggcaaaatc taatactaat aagcgctatg caatacgtaa actcggaaag atttttggtt
    10081 ctgcggtaat cgggctaact ttatttttag gaggaatctg aatacaagtt ttgctcatgc
    10141 tgctgatggg attcaatatg tcagagatga tactagagat aaagaagagg gaatagagta
    10201 tgatgacgct gacaatgggg atattattgt aaaagtagcg actaaaccta aggtagtaac
    10261 caagaaaatt tcaagtacgc gaattcgttt tgaaaaagat gaaacaaaat accgtagtga
    10321 aaatcctgtt acaattgacg gagaggatgg ctatgtaacc acgacaagga cctacgatgt
    10381 taatctagag actggtcatg ttaccgaaca ggttactgtt gatagaaaag aagccacgga
    10441 tacagttatc aaagttccag ctgaaagcaa ggttgaagaa gttcttgttc catgtgctac
    10501 taaatatgaa gcagacaatg acctttctgc aggacaggag caagagatta ctctaggaaa
    10561 gaatgggaaa acagttacaa cgataactta taatgtagat ggaaagagtg gacaagtaac
    10621 tgagagtact ttaagtcaaa aaaaagactc tcaaacaaga gttgttaaaa aatgaaccaa
    10681 gccccaagtt cttgtccaag aaattccaat cgaaacagaa tatctcgatg gcccaactct
    10741 tgataaaagt caagaagtag aagaagtagg agaaattggt aaattactct tactacaatc
    10801 tatactgtag atgaacgtga tggaacaatt gaagaaacta cttctcgtca aattactaaa
    10861 gagatggtaa aaagacgtat aaggagaggg acgagagaac ctgaaaaagt tgttgttcct
    10921 gagcaatcat ctattccttc gtatcctgta tctgttacat ctaaccaagg aacagatgta
    10981 gcagtagaac cagctaaagc agttgctcca acaacagact ggaaacaaga aaatggtatg
    11041 tggtattttt ataatactga tggttccatg gcaacaggtt gggtacaagt taatggttca
    11101 tggtactacc tcaacagcaa cggttctatg aaagtcaatc aatggttcca agttggtggt
    11161 aaatggtatt atgtaaatgc atcgggtgag ttagcggtca atacaagtat agatggctat
    11221 agagtcaatg ataatggtga atgggtgcgt taacagattc ctccctgcga caaaattgat
    11281 aatagtatgt ctgaatatat aattatatat aaaaagctat agataaggtc atcttatagc
    11341 gactataaaa aagtgaacaa gcctagtctt ctgtacatta gaattcagat ttgttcactt
    11401 tttgtttaat actcaatgaa aatcaaagag caaactagaa aactagtcgc aggttgctca
    11461 aagtactgct ttgaggttgt agatagaact gacgaagtca gtcacatata taatccaagg
    11521 tgacgttgac gcggtttgaa gagatttttg aagagtataa gtctttgatt ttgagactaa
    11581 ggagagtcag ggtcaaagta gtgaatgatt ctgaaataac tcataatagt ctcttgagag
    11641 gacagataag agtaacaaat atggtgctac tcatgtgtct tgcattttga tttacaaaca
    11701 gcgaagaaaa tataggaagc tgaaataaaa tatagatgaa tcaatttgga gagtcaacgc
    11761 aatctatggc aatattttcg aagctagggg gaagcatttt aatttcaatg tactaggcgt
    11821 cttctacagc tagctagagt ttcaattgta gatgttcttt gaaataatat cttagcgatt
    11881 tattggtatt agaagaatta gagaaattct ttctcaataa ataaacatgg cgtcaccaaa
    11941 actgaagaag cggtagtgtt cttggatggc atggtggtag gcatctaaga ctaattcacg
    12001 gcctgcaaag gcagaaacca acatgaccag agttgatttt ggcagatgga agttggttga
    12061 gaaggcatcc acgaccttcc actcataccc aggtttgata aagatattgg tccaaccaga
    12121 atctgcttgg atttgcccat caaacttgga accaatagtt tccaaggtgc ggatagaagt
    12181 ggttccgaca gcgatgacgc gaccaccatt ttctttgaca gagcgaaggg tggcagcagc
    12241 ttcctcagaa agttgataga actctgagtg catttcgtgt tcgtccagat tatccacaga
    12301 aacaggtcta aaggttccga gtccgacatg gagagtcaga tagactagat gaacaccctt
    12361 agcttggatt tctgccagca gttctttggt gaagtgaaga ccagcagtcg gtgctgcagc
    12421 agagccgctt tccttggcgt agacggtttg ataacgttca cggtcatcta atttttcgtg
    12481 gatataaggt ggcagaggca tttctcccag actttccaag acttctagga aaattccttg
    12541 gtattcaaag cggacaatgc gtcccccgtg ggtcaattct tctgtaacga cagcgctgag
    12601 gcggccatca ccaaagctga tacgagtacc gaccttgagg cgtttggcag gtttagccag
    12661 aacttcccac tcgtctccac tagtgttctt aaggaggaga agttccacat gacctcctgt
    12721 ctccactttt tgaccataga ggcgggcagg gagaactcgg gtgtcgttca tgacaagggc
    12781 atcaccaggt tccagcatat caataataga gtggaaatgt ttatcttgca tttctcctgt
    12841 ctcacggttg acgatgagga gtttggaggc atcacgtttt tcaaggggcg tttgggcaat
    12901 caattcctct ggcaagtgga aatcaaaatc agctgtattc atatatttgt tcattctttc
    12961 taagttctaa tcctatccat tataacacgt ttcaagtttg gacgctcttt ttttagaaat
    13021 taaatcgttt tcacttgaca aaaattggtc tataccatat aataaatata gacagaaatg
    13081 gaggatgaaa agatgaaagt tattaaagtt gaaaaccaag ttcaaggtgg aaaagttgct
    13141 tttgagattt tgaaggaaaa attggctaat ggagctcaaa ctctaggact tgcgacagga
    13201 agtagcccac ttgaatttta caaggaaatt gttgagagtg accttgattt ttcaaatcta
    13261 accagtgtca accttgatga gtatgtgggc cttgatgggg acaatccaca gtcttatcgt
    13321 tacttcatgc aagaaaactt gttcaaccaa aaaccattta aagaaagttt cttgcctcgt
    13381 ggtgttaagg acaatgctga agctgaagtt gaacgctaca accaaatttt ggctgaccat
    13441 ccagttgacc tccaaatctt gggaatcggt cgcaatgggc atatcggctt taatgagcct
    13501 ggtactccat ttgacagtca aacgcatcta gtagaacttg accagtctac tatcgaagcc
    13561 aatgcacgct tctttgccaa gattgaagac gtcccaaccc aagctatttc aatggggatt
    13621 aaaaacattt tggatgccaa gtctattatt ctttttgctt acggtgagtc gaaagcagaa
    13681 gccattgccg gaacagtatc tggcccagtg actgagaacc tacctgcaag tagcctccaa
    13741 aatcaccctg atgtaaccat catcgcagat gctgaagcgc ttagcttact cgaaaaataa
    13801 aaaagcaatg ttctattgaa cgctttcaac tcttgtataa atggaagaaa aaatcaaaat
    13861 taaaccgcat ttttgcttga caattattcc ttttacgtgt agaatgaaat agatcttgaa
    13921 cttgaaggga gtgaaaaaaa tgtctaaaac agtagtacgt aagaatgaat ctcttgacga
    13981 cgcacttcgc cgtttcaaac gtgcggttac taaagctggt actcttcaag aaacacgcaa
    14041 acgtgaattc tatgaaaaac cttctgtaaa acgtaaacgt aaatcagaag tagctcgtaa
    14101 acgtaaaaaa ttctaattga aatgaaaggc tagagaaatc tagtcctttt tcttttaaat
    14161 aaatactcca aagcctgcaa aaatctgaaa cttcctccta caatttgata taatagagag
    14221 aagaactcat ttgaaggagg aaatgatgtc ggttttagta aaagaagtga ttgaaaagct
    14281 tagactagac attgtctatg gtgaaccaga attgcttgaa aaggaaatca atacagcgga
    14341 tattacgcga cctggtcttg aaatgacagg ctattttgac tactatacac cagagcggat
    14401 tcaacttttg gggatgaagg agtggtctta tctgatcagc atgccttcca acagccgtta
    14461 tgaagttttg aaaaaaatgt ttctacctga gacaccagca gtcattgttg cccgtggttt
    14521 ggtggttcca gaggagatgt taaaggctgc tagagaatgt aagattgcta ttttaaccag
    14581 ccgtgcagct accagtcgtt tatctggaga gttatctagc tatctggatt ctcgtttggc
    14641 agaacgtacc agtgtgcacg gtgtcttgat ggatatttat gggatgggcg tcttgattca
    14701 gggagatagt ggaattggta agagcgagac aggtcttgag cttgtcaaac gtggtcaccg
    14761 tttggtagcc gatgaccgtg tcgatatctt tgccaaggat gagattactc tctggggtga
    14821 accggctgaa attttgaaac acttgattga aattcgtggg gttggtatta tcgatgttat
    14881 gagtctctac ggtgcgagtg ctgtcaagga ttcttcacag gttcagcttg ctgtctattt
    14941 ggaaaattac gatacgcata agacctttga tcgtcttgga aacaatgcag aggaacttga
    15001 agtttctggc gtagccattc ctcgtattcg tattccagtt aaaacaggtc gtaatatctc
    15061 tgttgtgatt gaggcagctg ccatgaatta tcgtgccaag gaaatgggct ttgatgctac
    15121 ccgtttgttc gacgaacgac tgacaagtct catagctcga aatgaggtgc aaaatgcttg
    15181 atccaattgc tattcaacta ggacccctag ccattcgttg gtatgcctta tgtattgtga
    15241 caggcttgat tcttgcggtt tatttgacca tgaaagaagc acctagaaag aagatcatac
    15301 cagacgatat tttagatttt atcttagtag cctttccctt ggctatttta ggagctcgtc
    15361 tctactatgt tattttccga tttgattact atagtcagaa tttaggagag atttttgcca
    15421 tttggaatgg tggtttggcc atttacggtg gtttgataac tggggctctt gtgctctata
    15481 tctttgctga ccgtaaactc atcaatactt gggattttct agatattgcg gcgcctagcg
    15541 ttatgattgc tcaaagtttg gggcgttggg gtaatttctt taaccaagaa gcttatggtg
    15601 caacagtgga taatctggat tatctacctg gctttatccg tgaccagatg tatattgagg
    15661 ggagctaccg tcaaccgact ttcctttatg agtctctatg gaatctgctt ggctttgcct
    15721 tgattctgat ttttagacgg aaatggaaga gtctcagacg aggtcatatc acggcctttt
    15781 acttgatttg gtatggtttc ggtcgtatgg tcatcgaagg tatgcgaaca gatagtctca
    15841 tgttcttcgg ccttcgagtg tcccaatggc tgtcagttgt ccttatcggt ctcggtataa
    15901 tgatcgttat ttatcaaaat cgaaagaagg ccccttacta tattacagag gaggaaaact
    15961 aaatgttaga agttgcatat attcttgttg ccctagcttt aattgtcttt ttggtctatc
    16021 tgatcattac tgtacaaaag cttggtcgtg tcatcgatga aacagaaaag acgattaaaa
    16081 ccttgacttc agatgtggat gtgaccttgc atcacaccaa tgagttgttg gctaaggtca
    16141 atgtcttggc agatgatatc aatgtcaagg tggctacgat tgatccactc ttcagtgctg
    16201 ttgcagattt atctctatct gtttcagacc tcaatgacca tgcgcgtgtc ttgagcaaga
    16261 aagcttcatc agctggttca aaaacactca agactggtgc aagtctgtca gctcttcgtc
    16321 ttgcaagtaa atttttcaaa aaataaaaaa ggagaatcct tatgggtaaa ttatcctcaa
    16381 tccttttagg aacggtttca ggtgcagctc ttgccttgtt tttaacaagt gataagggca
    16441 aacaagtttg cagtcaggct caagattttc tagatgattt gagagaagat ccggagtatg
    16501 ccaaggagca agtctgtgaa aaactgacag aagttaagga gcaggctaca gattttgttc
    16561 tgaaaacaaa agaacaggtt gagtcaggtg aaatcactgt ggacagtata cttgctcaag
    16621 ctaaatccta tgcttttcaa gcgacagaag catcaaaaaa tcaattaaat aatctcaagg
    16681 aacaatggca agaaaaagcc gaagctcttg atgactcaga agagattgtg attgatataa
    16741 cagaagaata aaccatcacc atctccggac ggactatgta tctggggatg gtgattttta
    16801 tctggaatct agtctttgtg gtataataat tactatgcag aaaaaaccaa cgtcagccta
    16861 tgtgcacatc ccattttgta cccagatttg ttattattgt gatttttcaa aggtcttcat
    16921 caaaaatcag ccagtcgaca gctatttaga gcatctgctg gaagagtttc gttcttatga
    16981 tattgaaaag ttgtcaaccc tttatatcgg tggtggaaca ccgacagccc tgtcggctcc
    17041 gcaactggag gttttactga atggcttgac taaaaacttg gacttgtctg tcttagaaga
    17101 gttgaccatt gaagccaatc caggcgattt gaatgcggat aagatagctg ttttgaaaaa
    17161 ttcggctgtc aatcgtgttt cgctaggtgt ccagaccttt gatgataaga tgttgaaaaa
    17221 gattgggcgc agtcatttgg agaaggatat ttatgaaaat atcgatcgcc tgaaactggc
    17281 tggttttgac aacatctcca ttgatttgat ttatgctctg cctggtcaga ccatggagca
    17341 agtaaaggaa aatgtggcta aagccattgg actggatatt ccccacatga gtttgtatag
    17401 tttgatttta gaaaaccata cggtctttat gaaccggatg cgacgtggga aattgcctct
    17461 gcctaaggag gaactagaag cggagatgtt tgagtacatc attgcagagc tggagcgagc
    17521 gggttttgag cattatgaga tttctaattt ctccaaaccc ggctttgaaa gtcgtcataa
    17581 tctcatgtac tgggacaatg ctgaatacta tggtattggt gcaggggcat ctggttatgt
    17641 caatggagta cgttataaaa atcatggtcc cattcgtcat tatctcagtg cggttgagga
    17701 aggcaatgct tgtattacag aagatcacct gagtcaaaag gagcaaatgg aagaagaaat
    17761 gttcttggga ctccgcaaga aatccggggt ttccatggcg cgatttgagg aaaaatttgg
    17821 acagtctttt gctggacttt atggagaaat tgtcagagat ttggttcaac aaggcctcat
    17881 gcagattgag ggtgaccacg ttcgcatgac aaagagaggt ctctttttgg gagatactgt
    17941 agcagaacga tttattttgg agtaggatca tgggcttaac ttatcaaatg aaaatgaaaa
    18001 ttccttttga tatggctgat atgaacggtc atatcaaact tccagatgtg attttgctgt
    18061 cactgcaagt ttcagggatg caatcgattg aactgggagt tagtgataag gccattttgg
    18121 aagactataa tctggtctgg attatcgcag aatatgatat tgaggtggtt cgtttgcctc
    18181 gttttgcgga agaaattacc atcgaaacgg aagccttgag ctacaatcga cttttttgct
    18241 accgtcgctt tactatttat aatgaagcgg ggcaggagct catccatatg atggcgacct
    18301 ttgttctcat ggaccgcgac agtcgaaaag tccatgctgt tgaacctgag attgtggctc
    18361 cttaccagtc tgattttgat aaaaaactta tccgtggacc gaagtatgaa tccttggaag
    18421 agccgatcag caaggattac catgttcgtt tttacgactt ggatatgaat ggtcatgtca
    18481 ataacagtaa atacttggac tggatttttg aggtcatggg agcggatttt ttgacccact
    18541 atattcccaa gaaaatcaac ctcaagtatg tcaaggaagt tcgaccaggt ggggtgatta
    18601 catcggctgt tgaacggact ggactggaaa gcaagcatga aattacaagt gatggggtta
    18661 tcaatgccca agcaatcatt acttggcaag aaataaaaaa ggtttaggga gaaatagatg
    18721 aaatataaag gctatttaat tgatttagac ggaaccattt ataaggggaa agaccgaatc
    18781 cctgctggag agacttttgt tcatgaattg caaaagcggg acattcccta tctctttgta
    18841 accaacaata caacccgcac tccagagagt gttaaggaga tgttggctca gaattttaat
    18901 atcgatacgc ctctatcgac tgtctacaca gcgactttgg caaccatcga ctatatgaat
    18961 gatttggggc ttgaaaagac tgtctatgtc gtcggagaag caggactcaa ggaagccatc
    19021 aaggcggctg gttatgtgga agacaaggaa aaccctgcct acgtggtagt tggtctggat
    19081 tggcaagtcg actatgaaaa atttgccaca gcaactcttg ctattcaaaa gggtgcccat
    19141 tttatcggaa ccaatcctga cctcaacatc ccgacggaac gtggtctttt gccaggtgct
    19201 ggctcactga ttacgctgct tgaagtagcg acacgagtga agccggttta tatcggaaaa
    19261 ccaaatgcca tcattatgga caaggcggtt gagcacttag gtttggaacg tgaagagtta
    19321 atcatggttg gggacaatta tttgactgat atccgggctg ggattgacaa tggcattcca
    19381 acgctcttgg tgacgacagg ttttactaag gcagaagaag tagctggcct gccaatcgca
    19441 ccgacacatg tggtttctag ccttgcggag tgggattttg atgaaaacta aattgacctt
    19501 ttggggctct atgctctttc tcctctccct ctcaatcctt ctgaccattt atctggcttg
    19561 gattttctat cctatggaga ttcagtggct aaacttaacg aatcgagtct atctaaaacc
    19621 agaaaccatt caatacaatt ttcatatctt gatgaattat ctgaccaatc cttttagtca
    19681 ggtcttacag atgcctgatt ttcgttcgtc agcagctggt ctgcaccatt tcgcagtggt
    19741 caagaatctc tttcatttgg ttcagctagt agctctagtg acactgccaa gtttctatgt
    19801 ctttgtcaat aggattgtga aaaaggactt tttgtctctt tatcgaaaaa gtctcctgac
    19861 tctagtagtc ttacctgtga tgattggact tgggggagtt ttgattggtt ttgaccaatt
    19921 ctttactctt ttccatcaaa ttctctttgt gggagatgat acctggcttt ttgatccagc
    19981 caaggatcct gttattatga ttttgccaga gaccttcttt cttcatgcct tcctcctctt
    20041 ttttgccctc tatgaaagct tctttgggta tctgtgtctg aaaagtcgta ggaagtgaaa
    20101 tgaaagatgt ttttaatttg catatataaa aatatatata tcgtttaaaa ccatcgttaa
    20161 gagtgaatta accaaatcca attgtgtcaa tcgttcgcaa acagcctatt tttcttgaaa
    20221 aaataggctt tttttctaaa aatccctagt caagcgcttt atttttttgt ataatagaag
    20281 tagcaaagta tagataagaa gagaaaagca tgattacact atttctatca ccgagctgta
    20341 catcatgtcg taaggctaag gcctggttag aaaaacataa ggttcccttt gtggaacaca
    20401 atattatgac cagtccttta acaagaaaag aattgcaaca catcctttcc ttgaccgaaa
    20461 atggtactga tgacatcatt tcaactcgtt caaaaatttt tcaaaaattg aatattgatg
    20521 tagagagtat ttcggtatcg gaattgcttc atttgattga gcagtatcct agtcttttgc
    20581 gtcgtccaat tattattgat gccaagcgta tgcaaatcgg ttttaatgaa gatgagattc
    20641 gtgcttttct ccctcgtagt tatcgtaagc aagaactaaa agaagcaaga atgagagctg
    20701 gtattagtta atgaacaaac agtatagtta cccactagat ttgtcgtgga gcactgaaga
    20761 acttgcttca gtgctttctt tttttaatga tgttgaagct gcctatgaag gcaaagtaga
    20821 ggctaaaaag ttactggact cctataaggg attcaaagcg gtcgtgccaa gcaagagcga
    20881 agagaaacgt ttgggacgag aatttgaaac ggttagtggc tattcacttt accgagcagt
    20941 tcaggctgcc aaagaaaaag gggaaggaaa gatttctctt ggaaagtaaa tttgaatttg
    21001 ctaaagagct tgttaagaaa gcaggccagt acattcttga ccatatgcag gaagacttgc
    21061 gtgttgaaac caagtcctct ccaacagatt tggtgaccag actggacaaa gaagttcagg
    21121 aactcttggt tggtgagatt ttgtcccgtt atcctgagga taagatttgt gctgaagaag
    21181 gttgtctgcg agcctcggtt caagagggca aggtttgggt cattgatccc attgatggta
    21241 ccaataattt tgtagcccag caggaagatt ttgctgttat gatggcttat tttgaaaatg
    21301 gtcagggaca gtttggtctg atttatgatg tggtcaaagg ggattgttac cacggtggtg
    21361 gggaatttcc agtttgtcta aatgataggc ccctagctcc ttttaaaact aaaccacttg
    21421 gagattttct cattgcaggg aatagtggta tgctggaaac caatgaatgg gggctggctg
    21481 atttgagccg agcggttctt ggtgttcgtg tctatggaag tgcggccatt agttttgcca
    21541 agattttgtc agggcgtttg ttgacctatc tcacttatct gcaaccatgg gattatgccg
    21601 cagctagtat tttaggggaa agtctgggct atcgggttgt gacccttttt ggtgaagctc
    21661 ctgattttca aaccagacag cctgtcatga tggtgcctct tgagatgcag gaggaaattc
    21721 agtcctatat ttacgaaagg aaaagaactt aaatgcaatt tccagaagga tttgttgaaa
    21781 aatataaaga aatactagga gatgaggcaa gagattttct tgcctctttt gaggaggaag
    21841 cggtttcggc ctttcgggtc aatcccttaa aagaagagca actttccttt tctgatgcca
    21901 ttactcaaac cccttggggc cactatggga aggtttcagg gaaatcgcct gagcatgcta
    21961 caggtttagt ttattcgcaa gaacctgctg ctcaaatggt ggctcaagta gcccaaccca
    22021 gtcctggtat gaaggtcttg gacttggctg ctgcaccggg gggcaaatca actcaactgg
    22081 cagcctatct agcaggggag gggctacttg tttccaatga aatttcaagc aaacgggcta
    22141 agattttggt tgaaaacatg gagcgttttg gagcgacaaa tgtcgtggtg accaatgaat
    22201 ctgccgaccg cttggtcaag gtctttaaag gctattttga cttaatcgtt cttgatgccc
    22261 cttgctctgg tgaaggaatg tttcgtaagc aacctgatgc tatggactat tggagcttag
    22321 attatccgag tcaatgtgct agcttgcaaa gagaaattct ggaggatgca gtgaccatgt
    22381 tagctgaagg tggtcgtctg gtttattcga cctgtacctg ggcacccgag gaaaacgaag
    22441 agattgtcaa ttggttgctg gaagagtatg attttgactt gttgccagta gagcatatca
    22501 atggaatggt agctggtatt gacctgccag aaacggctcg gatgtaccct catcagttta
    22561 agggagaggg tcagtttgtt gcccatctac agtttaaggg taacaatcca gctcctaaat
    22621 ttaaggcaag taagagtaac ctcagccgtg aacaagttgc cttgtggcag gaatttgccc
    22681 aaaaccattt gaaggtcaat ctaccgggta tcttgcagat ttttggagac cagctttatc
    22741 tcttgccaga acttttgcca gatttaggga agctcaagat tgctcgcaat ggactgcatc
    22801 tgggtacttt taagaagaaa cgctttgagc ccagtttcgc tcttggttta gccttgaaac
    22861 cgagtcaggt cgaacagtcg gttgaaattg gccaagaagc gtttgtcaag tatgcggctg
    22921 gagaaaccgt tcagctggca gaaagtctgc caaatggttg gtaccaagtt ttggttaaag
    22981 gcaatggctt gggctttgca aaggttactg gaaatgtttt gaaaaattat tttccaaaag
    23041 gcctcagatt caaatgaaaa tgctagtcaa atatcttatt ctatgttata gtgaaagtat
    23101 ggattttttt agtaggatga ttcttgatat cttgattgcc atcacatgta gttttactac
    23161 tcgatgaaaa tcaaagagca aactaggaag ctagccatag atagctcaaa acactgcttt
    23221 gaagagattt tcaaagagta caagaagttt agttattagc gttcttaccg cttgtaaact
    23281 agatttctca taaaatagaa tcttttcctt ttagttgtaa actagtctgg gagagtagag
    23341 aggtttgaga tacctttcta gcttttggat tatcatctaa gaagagtaat ttcccttgca
    23401 ttaaaaaggg gaaaaagaga cacgaaatga ctataatggg tgacaatggg ggaagggata
    23461 gacaagagat tttatccaca tatgaaaaaa ggaggttagg aaagagtaat atagcctata
    23521 ttatataaat aatcaattgc gcagaaattt ggtaagaatt catgcgtcaa ctcataaaga
    23581 actacttaaa aaattcacag tattcataat tattttcgag gagaaaaaca gtgaaaaaaa
    23641 gaaaaaagct tgctctgtct cttatcgctt tttggctgac ggcttgttta gtaggctgtg
    23701 ctagctggat tgatcgtgga gaatccataa cggctgttgg ctcaactgcc ttgcaaccct
    23761 tggttgaagt agcggcagat gaatttggca cgatccatgt tggaaaaacg gtcaatgtcc
    23821 aagggggagg ttctggtaca ggcttgtccc aggttcagtc tggggcagtt gatataggaa
    23881 actcagatgt atttgctgag gaaaaagacg gaattgatgc ttctgctctt gttgaccaca
    23941 aggtcgcggt agctggcttg gctctgattg tcaataagga ggttgatgtt gataacctaa
    24001 cgacagagca acttcgtcaa atcttcatag gtgaggtaac caattggaaa gaggttggtg
    24061 gtaaggactt acccatctct gttatcaatc gggcagccgg ctctggctct cgtgctacct
    24121 ttgatactgt cattatggaa ggtcagtctg ccatgcaaag tcaggagcag gattcaaatg
    24181 gagcggtaaa atcaatcgta tcaaaaagtc caggagctat ctcttattta tctcttacct
    24241 atatagatga ttcggtcaaa agcatgaagt tgaatggcta tgacttaagt ccagaaaata
    24301 taagtagcaa taattggccc ttgtggtctt atgagcatat gtatacattg gggcagccca
    24361 atgagttggc tgcagaattt ctcaattttg ttctctcgga tgagacccaa gaagggattg
    24421 tcaaaggatt gaagtatatt ccgattaagg aaatgaaggt tgaaaaagat gctgccggaa
    24481 ctgtgacagt gttggaaggg agacaataat gaatcaagaa gaattagcta agaaaatgtt
    24541 gcttccatca aagaattctc gtctggagaa attaggaaaa ggtttgacct ttgcctgtct
    24601 ttctttgata gtcatccttg tggccatgat tttggttttc gtagcgcaaa aaggcttgtc
    24661 gaccttcttt gtcaatggtg tgaatatctt tgactttctt ttgggaggaa cttggaatcc
    24721 ttctagtaaa gaatttggtg cccttcctat gattttgggt tcctttatcg ttaccattct
    24781 ctcagccctt atcgcaacac cctttgctat tggtgcagca gtttttatga ccgaagtatc
    24841 accaaaaggg gcgaagattt tgcaaccagc tattgaactc ctggttggga ttccttcagt
    24901 agtgtacgga tttattggct tgcaagtcgt cgttcccttt gttcgcagtg tctttggtgg
    24961 gactggtttt gggattttgt cagggatttc cgtcctcttt gtcatgattt tgccgaccgt
    25021 aacctttatg acaacggata gcttgcgtgc ggttcctcgt tattatcgtg aggctagttt
    25081 cgctatggga gccactcgct ggcagactat ctggcgtgtg atcttgaagg cggcccgttc
    25141 tggtattttc actgcagtgg tctttgggat ggcgcgtgcc tttggtgagg ctctagctat
    25201 ccagatggtt gtcggaaact cagctgttat cccaacttcc ttgaccacac cagctgcaac
    25261 tttaacttct atattaacta tgggaattgg gaacactgtc atgggaactg taaataataa
    25321 tgttctctgg tcactggcct tggtactgct cttgatgagc ttagttttta acagtgtgat
    25381 taaattgatt acgaaagaaa gaggaaagaa aaattatgcg cgctaagaaa ttagataaac
    25441 ttgcaacagc tgtcctctat acgattgcta gcatcattgt gacaatcttg gcttccttga
    25501 ttctctatat cttggttcgg ggcttgcccc atatctcttg gtctttcttg actggaaggt
    25561 cttctgcttt tcaagcaggt ggtgggattg gcattcagct ttacaattcc tttttcctat
    25621 tggtcattac cttgattatt tctgtacctc tttctatggg agctgggatt tacttggctg
    25681 aatatgctaa aaaaggtcct gttaccaact ttgtgcgaac ttgtattgaa atcttgtcct
    25741 ctttaccatc agtggtggtg ggtctctttg gttacttgat ctttgtagtc cagtttgagt
    25801 atggattttc aatcatttca ggtgctttgg ccttgacagt ctttaacttg cctcagatga
    25861 cgcgtaatgt agaagatagt ttgaaacacg ttcaccatac ccaacgtgag gctggtctgg
    25921 ctcttgggat ttctcgctgg gagacagtgg ttcatgttgt tattccagaa gcgcttccag
    25981 gtattgtaac gggtgtcgtc ttggcatctg gtcgtatctt tggcgaagct gcagctctga
    26041 tctatacagc agggcaatcg gcgccagctc ttgactggtc taactggaat atcctcagtg
    26101 tgactagccc catctctatc ttccgtcaag cagaaacctt ggctgtccat atctggaaag
    26161 tcaatagtga aggcactatt ccagatggaa ccattgtatc agcaggttct gccgctgtgc
    26221 tcctgatctt tatcctgatt tttaactttg gagctcgtaa gttcggaagc tatctacaca
    26281 agaaattaac cgctgcctaa aggagaagcc atgtcaacat ataactggga tgagaagcat
    26341 atccttacct ttcctgaaga aaaagtagcc ctttctacta aggatgtcca tgtttattat
    26401 ggtaaaaatg aatccattaa ggggattgat atgcaatttg aaagaaataa aattacagct
    26461 ttgattggtc cgtcgggatc ggggaaatct acctacttac gcagtctcaa tcgcatgaat
    26521 gataccattg atattgctaa agtaactggg cagattctct atcgtggaat tgatgtcaac
    26581 cgtccagaaa tcaacgttta tgaaatgcgt aaacacattg gaatggtttt tcaacgcccc
    26641 aatccatttg ctaagtcaat ttaccgtaat attacctttg cgcatgaacg tgctggagtt
    26701 aaggataagc aagtcctaga tgaaatcgta gaaacctccc ttcgtcaggc tgccctctgg
    26761 gatcaggtta aagacgatct ccacaagtca gccttgacct tatcaggtgg tcagcaacaa
    26821 cgtctctgta tcgctcgtgc catctctgtt aagccagata tcctcttaat ggatgagcca
    26881 gcctcagcct tggatccgat tgcgaccatg caactagaag agaccatgtt tgagctcaag
    26941 aaaaacttta ccatcatcat tgtaacgcat aatatgcagc aggctgctcg tgcaagtgac
    27001 tatacaggct tcttttactt gggtgatttg attgagtatg acaagactgc aactattttc
    27061 caaaatgcca agctacagtc caccaatgac tatgtatctg gtcactttgg ttagaaagga
    27121 aaccgtatga cagatgcgat tttacaggta tcagacctgt ccgtttatta taataaaaag
    27181 aaggctttga atagtgtttc cctatctttc caacctaagg aaattacagc cttgattggt
    27241 ccatctggat cagggaagtc aaccctcctc aagtctctca accgcatggg agatctcaat
    27301 ccagaggtga ccacaactgg atccgtggtg tacaatggtc acaacatcta cagtccgcgt
    27361 acagatacgg ttgaattacg taaggaaatc ggaatggttt tccaacaacc taatcctttc
    27421 cctatgacta tctatgagaa tgttgtctac gggcttcgta tcaatggaat taaggataag
    27481 caggttctgg atgaagccgt agaaaaagcc ttgcaaggtg cctctatctg ggatgaggtc
    27541 aaggatcgtc tatatgattc agctattgga ttgtcaggtg gtcaacagca gcgtgtctgc
    27601 gtggcccgtg tcttggcaac tagtcctaaa atcatcctct tggatgagcc aacttcggct
    27661 ttggatccga tttcagctgg taagattgag gaaaccttgt atggtctaaa agacaagtac
    27721 accatgcttc tggtaacccg ttccatgcag caagcttcac gtatctctga taagacagga
    27781 tttttcctag atggagattt gattgaattt aatgatacca agcagatgtt ccttgatccc
    27841 caacacaagg aaacggaaga ctatattaca ggaaaatttg gataaggaga tgaaagatgt
    27901 tacgatctca atttgaagaa gatttagaga aattacataa ccagttctac gctatgggac
    27961 aagaagtgct ctcacaaatc aatcgtacgg tacgtgcttt tgtcacgcat gaccgtgacc
    28021 tggcaaaaga ggtcatagaa gatgatgcag aagtaaatga atacgaagtg aaactggaaa
    28081 agaaatcatt tgaaatgatc gcactccaac aaccagtctc tcaagatttg cgtacagtct
    28141 tgactgtcct taaggctgta tcagatgtgg agcgtatggg ggatcacgct gtagccattg
    28201 cccaggcaac catccgtatg aagggggaag agcgcatccc agctgtagag gaagaaatta
    28261 aaaaaatggg acgtgaagtt aaaagcgttg ttgaagcagc acttgatctt tatcttaatg
    28321 gttctgttga cgacgcatac cgggtggcct ccatggatga gcaaattaac cactattttg
    28381 aaactatccg tgaccttgcg actgaagaga ttaagaaaaa tccagaagcc attgttacgg
    28441 gtcgtgatta tttccaagtt atttcctact tggagcgtat cggtgactat gctaaaaata
    28501 tctgtgaatg ggttgtctac tttgaaacag gtaagattgt cgaactataa aatagtttaa
    28561 ttctgtataa aaaggagctt gaaatcaact gatggctcct ttttttgaac gaaaaaatat
    28621 ttttgagata aaaatttaaa aaacacttga caagtaactt ggatatcgtt ataattatac
    28681 aaaattaaca gagaggttgt ttatttatga aatcaaaaaa atggatattt gttttatgta
    28741 attttcttgc aagtttcttc ttagtagctt gccagtcagg ttctaatggt tctcagtcag
    28801 ctgttgaggc cattaagcaa aaggggaaat tagttgtggc aaccagtcct gactatgcac
    28861 cctttgaatt tcaatcattg gttgacggaa agaatcaggt agtcggtgca gacatcgaca
    28921 tggctcaggc tatcgctgat gaacttgggg ttaagttgga aatctcaagc atgagttttg
    28981 acaatgtttt gaccagtctt caaactggta aggctgacct agcagttgca ggaattagtg
    29041 ctactgacga gagaaaagaa gtctttgatt tttcaatccc atactatgaa aacaagatta
    29101 gtttcttggt tcgtaaggct gatgtggaaa aatacaagga tttaactagc ctagaaagtg
    29161 ctaatattgc agcccaaaaa gggactgttc cagaatcaat ggtcaaggaa caattgccaa
    29221 aagctcaatt aacttcccta actaatatgg gtgaagcagt caatgaattg caggctggaa
    29281 aagtagatgc tgttcatatg gatgagcctg ttgcacttag ttatgctgct aaaaacgctg
    29341 gcttagctgt cgcaactgtc agcttgaaga tgaaggacgg cgacgccaat gccgttgctc
    29401 ttagaaaaaa tagtgatgat ttgaaagaag tggtagacaa ggtcatccaa aaacttaagg
    29461 atgaaggaac ttaccaaagc tatcttgaaa aagcagcaag tctaactgaa gttgaagaat
    29521 aagaaaaagc agagttggat gtcattccaa ttctgctttt aaatagttta aaacattttc
    29581 cagattgtct agggacacct tggcaaattg ataaggacag gtgcttaaat aggcctcttc
    29641 aaagaagtcc aagcccagtt gactgtgttt gagactggcg attttaggat actgtctcag
    29701 gtctagcaat aaaccatcgt gtaggggata atgaggaagg ggacaaggtg ttttagttat
    29761 cctttcctcg atttgttttt ggtaagattc atgattggtc aagcgagcca aacgattttt
    29821 ttcaaacaat tgactgtcaa tatagagtga cagggccttt tgcataatga ggttgctgtc
    29881 gtagtctagg atatttttgt aggccacaaa tgcttctttg atagcatttg gaagaatgag
    29941 tgctgtaata cgaagggcag gaaaaaggct ggtcgagaag gacttgatat aaatgacacg
    30001 ctcctctgta tcaagatagt ggaaggtttg gcccttcttg gagtccaaat cacccagata
    30061 atcgtcctct acgatataga catcatactt ggcagctaag ttaagaatag atcgtttgtc
    30121 ctgctcagaa taggaatgtc ccagaggata gtgaaatcgg ggaatggtgt agaaaaattt
    30181 aatttttcct gttttgaagt ggccttccag ctcctccaag tcaatcccat caatgcctcg
    30241 ttcaatcgtt tgatagtcca gcccctgtgc aatcaagagg cgattcatcc gatggtaggt
    30301 tggctgttcc accaagattt ccttggcttg tctaggaaag gatatttgag agaggataaa
    30361 caaggcttgt tgggttccag aagtcagtac tagttggtta gccttgcagt agagagcttg
    30421 ctcaaagagg agtttgtgaa tggactgtct taggtcttct aatccttctt gattgtcata
    30481 gtagttgaag aggtagtttt ctcggccaat caaggtttca ttgacacaga gtcggaaatc
    30541 gtcataggca ctggcatgtt cgtcggtaac ctcgatttct aggtcttgat gttgcccttg
    30601 ttctaataca tagtagccac tctgaggctt ggcatagagg tattgttcgt gccgtaattc
    30661 cagcagggct cgttgaatgg tgtccttgct gcagtgaaag tcaaggctca gttgacggat
    30721 agaaggcagg cgactacccg tcggaaatcg ttcagactcg ataccatttt tcagatagga
    30781 aacgacctct ttgtacttgc tttgtttctt cattccagac ctcccttgat tttgttacat
    30841 tgtacccttt tttttccttc ttggcaatgt ctagagcgtt tctctcgttc acatctaggg
    30901 gcaaatgtgg tatacttagg agtaagattt atagaataac agacaagaaa gtgaatggat
    30961 aagaacgtgc aggaaccagt gaaattattt caatacaata ctcttggagc cttgatggct
    31021 ggcctttatg gtggtactat gacagtagga gaattgctag aacatggtga ccttggtttg
    31081 ggaaccttgg attccattga tggggaattg attgtcttgg atggcaaggc ttatcaggcc
    31141 aaagggtcag gagaccagcc agaaattgtg gaagtgtcac cagatgccct tattccttac
    31201 gctgcagtgg taccgcatca ggcagaggtc attttccgcc agcgctttga gatgacagac
    31261 aaggaattga aagaacgaat tgagtcttat tatgatgggg aaaatctttt ccgttctatc
    31321 aagattcggg gggaattttc acatatgcat gtgcgtatga ttcctaagtc aacacccgat
    31381 accaagtttg ctgatgtcgc aacccatcaa ccggaatata gtcgtgacaa tgttgcgggg
    31441 actattgttg gtttctggac gcctgagatt ttccatgggg tcagtgtggc aggctaccat
    31501 ctgcacttca tatcagatga tttgaccttc ggtggacatg tcatggattt tgtcatcaag
    31561 gaaggcatta tcgaggtggg agcagttgac cagttggacc aacgtttccc agtccaagac
    31621 cgtcaatact tgtttgctaa gttcaatgtt gacgagatga aaaaagatat tgaaaaggca
    31681 gaataggaga agaaaatgac cattcatatc attattacca tgttgctgtt gctagctttt
    31741 ctgataggaa gcatttggtt tgccaaaaag aaataccaga ttaatctagc tgtcttgggc
    31801 ttgggtgcag tcgctttctt tgtctcttcg cagattttgg aaaaactggt gcatatcttg
    31861 attttgcatc ctcaaaaaag acggtagtat tgccctcttg caagatcacc cgcttgtcta
    31921 tattatctat ggcctagcta tggcagcatt ttttgaggaa acggctcgtc ttgttttctt
    31981 caaatggttg gagaaaaaga gaagtttgga aaaggcagac gccttggcct atggactggg
    32041 gcatggtggc ttggagttga tttttctagg ccttactagt ttactcaatc tttacattgt
    32101 tctttcggca gttcaaactc agaatccaca ggttatgcaa ttactgtctg aaaatatgtt
    32161 aaaaaccatt cagtcacttt cggtctggca gatttatttg cttggttttg agcgaatctt
    32221 ggcgcttggt ttccaattac ttttgacagt ttgggtttac caagctgttc gccagaagaa
    32281 atggatttat ctcctagcag cctatggctt gcatgccttc tttgatctgg caccatctct
    32341 ttcccaagta ggctggttga caaatccagt cttggttgaa gtgattctag cactggagct
    32401 cgttctggtc gcctatggaa ccaaggaaat cttttgtaaa aaatcataaa aagggggaaa
    32461 cctctttttc ttatgcaaaa tccaaacaag gtatttttat ggtcgtcaaa tgtctctaaa
    32521 aatggtataa tggaatgaat tttgtaaaag gaagagtgac atgtctgtaa gagaaaaaat
    32581 gcttgaaatc ttagaaggaa ttgatatccg ttttcaggaa cccttgcata gctatagtta
    32641 tacaaaagta ggtggagagg ctgattattt ggtctttcca cgaaatcgtt ttgagttggc
    32701 tcgcgttgtg aaatttgcca accaagaaaa tatcccttgg atggttcttg gcaatgcaag
    32761 caatatcatc gttcgtgatg gtgggattcg tggatttgtc atcttgtgtg acaagctcaa
    32821 taacgtttct gttgatggct ataccattga agcagaagct ggggctaact tgattgaaac
    32881 aactcgcatt gccctccgtc atagtttaac tggctttgag tttgcttgtg gtattccagg
    32941 aagcgttggc ggtgctgtct ttatgaatgc gggtgcctat ggtggcgaga ttgctcacat
    33001 cttgcagtct tgtaaggtct tgaccaagga tggagaaatc gaaaccctgt ctgctaaaga
    33061 cttggctttt ggttaccgcc attcagctat tcaggagtct ggtgcagttg tcttgtcagt
    33121 taaatttgcc ctagctccag gaacccatca ggttatcaag caggaaatgg accgcttgac
    33181 gcacctacgt gaactcaagc aacctttgga atacccatct tgtggctcgg tctttaagcg
    33241 tccagtcggg cattttgcag gtcagttaat ttcagaagct ggcttgaaag gctatcgtat
    33301 cggtggcgta gaagtgtcag aaaagcatgc aggatttatg atcaatgtcg cagatggaac
    33361 ggccaaagac tacgaggact tgatccaatc ggttatcgaa aaagtcaagg aacactcagg
    33421 tattacgctt gaaagagaag tccggatctt gggtgaaagc ctatcggtag cgaagatgta
    33481 tgcaggtggt tttactccct gcaagaggta gtggggacct gacagagccc cgatcggtta
    33541 atctatgaaa aagaaggaat ttatgccaat tgaaaaaacc aattatcgaa ttcaaaaacg
    33601 tctctaaagt ttttgaagac agcaacacca aggttctcaa agacatcaac tttgagttgg
    33661 aagaagggaa attctacacc cttctaggtg catctggttc ggggaaatca actatcctaa
    33721 acattattgc aggtttactg gatgcgacga caggagatat catgctagac ggtgttcgta
    33781 tcaatgatat tccaaccaac aagcgcgacg tacataccgt cttccaatcc tatgccttgt
    33841 tcccacatat gaatgtgttt gaaaatgttg cctttccact tcgcttgcgt aaaattgata
    33901 agaaagaaat cgagcagcgt gtagcggaag ttctcaagat ggttcagttg gaaggttatg
    33961 aaaaacgttc catccgcaaa ctttctggag gacaacgtca gcgtgtggcc atcgcccgtg
    34021 ctatcatcaa ccaaccccgt gtggtcttgt tggacgagcc tttatcagcg ctggacttga
    34081 aattgagaac agacatgcag tacgaattgc gtgaattaca acaacgattg ggcattacct
    34141 ttgtctttgt cactcacgat caggaagaag ctcttgccat gagtgactgg attttcgtta
    34201 tgaatgatgg cgagattgtc cagtctggaa cccctgtgga catctacgat gagccaatca
    34261 accactttgt tgccaccttt atcggggagt caaacatctt gccaggtacc atgattgagg
    34321 actacttggt cgaatttaac ggcaaacgct ttgaagcggt tgatggtggg atgaagccaa
    34381 atgaacctgt tgaggtcgtt attcgtccag aggacttgcg cattaccctt cctgaagaag
    34441 gcaaactcca agttaaggtc gacacccagc tcttccgtgg agttcattat gaaattatcg
    34501 cctatgacga acttggaaat gaatggatga tccactcaac ccgtaaggcc atcgtgggtg
    34561 aggaaatcgg tctggacttt gaatcagaag acatccacat catgcgtctc aatgaaaccg
    34621 aagaagagtt cgatgctcgt atcgaagaat acgtggaaat cgaagagcaa gaagcaggtc
    34681 tgatcaatgc gatcgaggag gaaagagatg aagaaaacaa gctctaaact ctttgtagtg
    34741 ccctacatgc tttggattgc cctctttgta ttggcaccct tggtcttgat tttcggacaa
    34801 tcctttttca acattgaagg ccagtttagt ttagaaaatt ataaatctta ctttgcgtca
    34861 caaaacttga cctacctcaa aatgagtttc aactcagtgc tttatgcagg cattgtgacc
    34921 tttgtgacct tgcttatcag ctatccaaca gccctctttt tgacccgtct caagcaccgt
    34981 caactctggc tcatgctgat tatccttcct acctggatca atttgctcct taaagcctat
    35041 gcttttatcg ggatttttgg tcaaaatggc tctattaacc aattcttgga atttatcgga
    35101 attggttcac aacagttgct ttttaccgat ttttccttta tctttgtcgc aagctacatc
    35161 gagctcccct ttatgatttt gccgattttc aatgtcttag acgatatgga taataatctc
    35221 atcaatgcta gttatgacct tggtgcaact aagtgggaga ccttccgtca tgtcatcttc
    35281 cctctatcta tgaacggtgt gcgaagtggg gttcagtcgg tctttatccc aagtttgagt
    35341 ctcttcatgc tgacccgttt gattggtggg aaccgcgtta tcaccttggg gacggctatt
    35401 gagcagaatt ttctaaccaa tgacaactat ggtatgggtt caaccatcgg tgtgattctc
    35461 atcctgacca tgttcatcac catgtgggtg actaaggaaa ggagagaacg atgaaaaaat
    35521 ttgccaacct ttatctggga ctggtctttc tggtcctcta cctgcctatc ttttacttga
    35581 ttggctatgc ctttaatgct ggtgatgata tgaatagctt tacaggtttt agctggactc
    35641 actttgaaac catgtttgga gatgggagac tcatgctgat tttggctcag acatttttct
    35701 tggccttcct atcagctttg atagcgacca ttatcgggac ttttggtgcc atttacatct
    35761 accagtctcg taagaaatac caagaagcct ttctatcact caataatatc ctcatggttg
    35821 cgcctgacgt tatgattggt gctagcttct tgattctctt tacccaactc aagttttcac
    35881 ttggcttttt gaccgttcta tctagtcacg tggccttctc cattcctatc gtggtcttga
    35941 tggtcttgcc tcgactcaag gaaatgaatg gcgacatgat tcatgcggcc tatgacttgg
    36001 gagctagtca atttcagatg ttcaaggaaa tcatgcttcc ttacctgact ccgtctatca
    36061 ttactggtta tttcatggcc ttcacctatt cgttagatga ctttgccgtg accttctttg
    36121 taacaggaaa tggcttttca accctatcag tcgagattta ctctcgtgct cgcaagggga
    36181 tttccttaga aatcaatgcc ctgtctgctc tagtctttct ctttagtatt atcctagttg
    36241 taggttatta ctttatctct cgtgagaagg aggagcaagc atgaaaaaaa tctattcatt
    36301 tttagcagga attgcagcga ttatccttgt cttgtgggga attgcgactc atttagatag
    36361 taaaatcaat agtcgagata gtcaaaaatt ggttatctat aactggggag actatatcga
    36421 tcctgaactc ttgactcagt ttacagaaga aacaggaatt caagttcagt acgagacttt
    36481 tgactccaac gaagccatgt acactaagat aaagcagggt ggaacgacct acgatattgc
    36541 cattccaagt gaatacatga ttaacaagat gaaggacgaa gacctcttgg ttccgcttga
    36601 ttattcaaaa attgaaggaa tcgaaaatat cggaccagag tttctcaacc agtcctttga
    36661 cccaggtaat aaattctcca tcccttactt ctggggaacc ttaggaattg tctacaacga
    36721 aaccatggta gatgaagcgc ctgagcattg ggatgacctt tggaagccgg agtataagaa
    36781 ttctatcatg ctctttgatg gggcgcgtga ggtgctggga ctaggactca attccctcgg
    36841 ctacagtctc aactccaagg atctgcagca gttagaagag acagtggata agctctacaa
    36901 actgactcca aatatcaagg ctatcgttgc ggacgagatg aagggctata tgattcagaa
    36961 taatgttgca atcggcgtga ccttctctgg tgaagccagc caaatgttag aaaaaaatga
    37021 aaatctacgt tatgtggtac cgacagaggc cagcaatctt tggtttgaca atatggtcat
    37081 tcccaaaaca gttaaaaacc aaaactcagc ctatgccttt atcaacttta tgttgaaacc
    37141 tgaaaatgct ctccaaaatg cggagtatgt cggctattca acaccaaacc taccagcgaa
    37201 ggaattgctc ccagaggaaa caaaggaaga taaggccttc tatcccgatg ttgaaaccat
    37261 gaaacaccta gaagtttatg agaaatttga ccataaatgg acagggaaat atagcgacct
    37321 cttcctacag tttaaaatgt atcggaagta ggagataaac gtaagaagac gaatcagtca
    37381 agctggttcg tttttaagga atagaagtca gtttttagag ccctatcttt ttctattcta
    37441 gttgtttatt tgtttatttg cttatttgct tgcctagtag tatactaagc ctatactctt
    37501 caaaccacgt cagcgttgcc ttgccgtact caagtacagc ttgaggctag cttcctagtt
    37561 tgctttttga tttttattga gtattaacct tacagaaagc ggcaacaaat aatgaaaatg
    37621 tctacttttt tcaaaaaaag tttctggcca acctttatga ttgttaacca aactgctata
    37681 ctattccatt taaaggatgg cttggatcga caatacctaa caactgagtc catatattgg
    37741 gtgataggca cctttatatt tggaaatatt ctagtagctg tgtttagtaa tatgaaaatt
    37801 tgggataaaa agaaaaacgg cagtaagaaa aaatatatcc tgaaaaagta atcagagatg
    37861 tagggaattg gtctaattca acggagatca tttccagttg acgcaagctt aaaaaaacgt
    37921 tataataaga aagttgagaa ttgattttca cttgtcttag tccagagaaa tggcggtgct
    37981 gcgagccatc taagctagga agtcatgctg ctcaatcata caattgcata agaacaagag
    38041 aatgacaagt tcattgaatg aaggtggtac cgcggttttt cgcccttcgt gatatgagct
    38101 tgtcttttga tttttggagg tgttgatgaa aacatttctt gtcaaacaaa agtttcgtct
    38161 tggaggcgaa cgcttcgcta tcaaggatga caggggagaa attgcctatc aggtggaggg
    38221 atcatttttt aagattccta aaacctttac catctatgat gcggctggtg aacaagtcag
    38281 tgagattagt aaagaaatct tgaccttgct tcctcgtttt gagattcagc ttcgggatgg
    38341 ctcgagtttt gtcattcgta agaagttgac cttctggcga gataagtatg agtttgataa
    38401 tctaggtctt cgtatcgagg gcaatatctg ggatttgaat ttcaaattgc tggatgatcg
    38461 cgatcagttg attgcggaaa ttaagaagga actcttccat ctgacctcta cctataccgt
    38521 aacggttctt gaagacgctt atgcagacct agtcatttcc ctctgtgtcg cgattgacta
    38581 tgtggaaatg ctggaaagcc aatcacatta aacaagtaaa taaggagaca atatgaaaca
    38641 actatctagt gcacaagtac gccaaatgtg gcttgatttc tgggcgacca aaggtcactc
    38701 agtagaacca tcagtaagtt tggttcctgt aaatgaccca actcttttgt ggatcaactc
    38761 tggggtagca acccttaaga aatactttga cgggaccatt atccctgaaa atccacgtat
    38821 taccaatgcc caaaaggcta tccgtaccaa cgacatcgaa aacgtaggga agactgcgcg
    38881 tcaccatacc atgtttgaaa tgttggggaa cttctctatc ggggattact tccgtgacga
    38941 agctatcact tgggcttatg agcttttgac aagccctgaa tggtttgatt tccctgctga
    39001 aaaactttac atgacctact atccagacga taaagattct tacaaccgct ggattgaagt
    39061 gggagtggat ccaagtcact tgattccaat tgaggacaac ttctgggaaa tcggtgcggg
    39121 accttctgga ccagatacag aaatcttctt tgaccgtggg gaagcctttg acccagaaaa
    39181 tatcggtctt cgcctgcttg cagaagatat tgaaaacgac cgttatattg aaatctggaa
    39241 catcgttttg tcacaattta acgcagaccc tgctgttcct cgtagcgaat acaaggaatt
    39301 gccacataag aacattgata cgggcgctgg tttggagcgt ttggtggctg ttatccaagg
    39361 ggctaagacc aactttgaaa cggacctctt tatgccgatt atccgtgaag tcgagaaatt
    39421 gtctggtaag gtttatgacc aagatggcga caacatgagc ttcaaggtta tcgcagacca
    39481 cattcgttct ctttcatttg ctatcggtga tggtgccctt ccaggaaatg aaggtcgtgg
    39541 ttatgttctt cgtcgtcttc tccgtcgtgc ttctatgcat ggtcaaaaat tgggtatcaa
    39601 cgagcctttc ctttacaaac ttgttccaac tgttggaaac atcatggaaa gctactaccc
    39661 agaagtgctt gagaaacgtg actttattga gaaaatcgtt aagagcgaag aagaatcatt
    39721 tgcccgtacc cttcactcag gtcaacactt tgcccaaggc attgtggcag acttgaaaga
    39781 aaaaggtcaa tctgtcattg ctggtcaaga tgtctttaaa ctctatgata catacggatt
    39841 cccagttgaa ttgactgaag aaattgctga agaagctggt atgactgtag accgtgaagg
    39901 ttttgaagca gccatgaaag aacagcaaga acgcgcgcgt gcgtcagctg tcaagggtgg
    39961 ctcaatgggt atgcaaaatg aaactcttca aaacatcact gtagaaagtg tcttcaacta
    40021 caatgctagc caattgtctt ctaaattggt agctatcgtt gctgacaatg cagaagtagg
    40081 agctgtttca gaaggaactg cctctcttat ctttgcggaa acgtcatttt atgctgaaat
    40141 gggtggacag gtagctgact acggacaaat cttggatgag tcaggtaagg ttgtggctac
    40201 tgtgaccaat gttcagaaag ccccaaatgg tcaagccctt catacagttg aagtccttgc
    40261 accgcttgcc ttgaaccaag aatatacctt ggcaattgat agcaatcgcc gtcaccgtgt
    40321 catgaaaaac cacactgcga ctcatttgct tcacgctgcc cttcacaata tccttggaaa
    40381 ccacgcaaca caggcaggat ctcttaacga agttgaattc cttcgctttg actttaccca
    40441 cttccaagca gtaactgctg aggaacttcg tgcgattgaa cagcaagtta acgagaaaat
    40501 ttgggaagca cttgaagtga agacagttga aacggatatt gacactgcta aagaaatggg
    40561 agctatggcc ctctttggtg agaaatacgg caaggaagtt cgtgtcgtga ctatcggtga
    40621 ctactctatt gaactttgtg gtggtactca tgttggcaac acttctgaga ttggtctctt
    40681 caaaattgtc aaagaagaag gaatcggttc aggaactcgc cgtatcttgg cagtgactgg
    40741 taaggaagcc tttgaagcct atcgtgaaca agaggatgct cttaaagctg tcgcagcaac
    40801 cttgaaagca cctcaagtca aggaagtacc tcacaaggta gaaggacttc aagaacaact
    40861 tcgtcaactt caaaaagaaa atgctgagtt gaaagaaaaa gccgcagctg cagccgcagg
    40921 cgatatcttc aaagatgcta aggaagtcaa cggtcatcgt tacattgcta gtcaattgtc
    40981 tgtatccgat gccggtgccc ttcgtacttt tgcagataac tggaaacaaa aagactactc
    41041 tgatcttctt gtcctagttg ccgctatcgg tgacaaagtc aatgtccttg tagcaagcaa
    41101 gacaaaagac cttcatgcag gaaaccttgt caaagaatta gcaccaatca tcgatggacg
    41161 tggtggtggt aaaccagaca tggccatggc aggaggaaga aaccaagcta agatccaaga
    41221 attgttggat gcagtagcag gtaaattgta atcaagtaaa gatctatcca tttgggtagg
    41281 tctttttgtg aatacaaaaa agccaaatcc gcttggacct agcttggtta ttatgagcta
    41341 ttagatggta ttgactgccc agacacttac ggatctagct gagacaggga attgtccata
    41401 accttcctca tcaattgtaa cttgaccttg gtggttacca agtaaatcta caaaggtttg
    41461 attagtccat tcttgaccga caaacattga cttgctgttt tcttggtcat ttgagataag
    41521 gactgcgatt ggggattgat tttcagcacc tgaacgtacc caaccgatac agttagcatg
    41581 gtcaaagtag tcattttgtt ctccataggc caagtctttt cggatggcta ggaggcggtc
    41641 aaggatttct ttgaaatctt cttgagcata ctggcctgaa atcccatagt agtctccgta
    41701 aaagacacat ggaaggccgt cttggcgtaa caaaatgagg gcataggctg ctggcttgaa
    41761 ccattcttca acggtagact caagggcttg tcctcgttgg gtatcgtggt tgtcgataaa
    41821 agtcacagcc ttgtcaggct tgagttcaac caggctatct gtgaaaatgc cacgaaggtc
    41881 atagtttgcg ccagcttgac tggcttcaaa gagattctgg tggagacgaa catcgacaag
    41941 gtcaaagtgt tcttccgttt tttcgagata gtccagattg gcttccttgt ctgggttcca
    42001 aaattcacca aaaacataga aatcgtcacc gtatttttcc ttcatatcgc ggatgaaatt
    42061 gcgcataaag aaagagtcaa tatgcttaac ggcatccaaa cggaaaccag ctacaccagt
    42121 cgtttccatg aaccaatcag cccagtcata gatgttttgg atgacttcag gatgtttaaa
    42181 gtctaggtcg gcatacatga ggtagtcgta gtttccgttt tcgttatcga ccaattcctc
    42241 gttagcccag cccttgttgt ctccttggat taggtaaatc ccggatttac ggcgtttggc
    42301 atcatagtct gtacctgtga agtggtacca gtgccagtgg aagtcattgt aggtatcttg
    42361 gcgaccatcg aaggtaaaac tagtccagcc attgatggtg aagggttctc caagttcaac
    42421 tgtacggtct acaggatcca cttcaataac ctgaaaggct tccctgtgat cagcagcagc
    42481 cttgtggttg agaactacat cggccatagg ttgaattccc tgcgctttaa gggcttgaat
    42541 ggcttgaaga tagtcttctt tgaaaccata cttggtgcgg acagtccctt tttggttgaa
    42601 ctctcctaag tcaaataagt catagacccc atagccgaca tctttttcgt tggttgcctt
    42661 gaaggctggt ggcatccaga cgtgactgat ccccagatga gctaggtgtg gagcattttc
    42721 agccagacgc gtccagtgtt gaccgtcgtg gggcagatac cattcaaagt attgcatgag
    42781 tgtttgattt tgcatgatgt ttcctcttac ttgtcaatct tgttctttat tctatcataa
    42841 agtatctctc ggagcaaacg tttgcgtaag atagaaggaa actattttaa ctacttaaaa
    42901 taaagtattg atttttttgt atcaaagtgc tagtatagcg atatataata cagataaaga
    42961 taggagtcat cccatgattg aatttcaaaa tgttagtaag ttgtatggtg ataaagaggc
    43021 cttgagtaat ctcaatttgc agattgaaaa tggagagatt atgggcttga ttggtcataa
    43081 tggggctgga aaatcgacca ccataaaatc cctagtcagt atcatttcac ccagcagtgg
    43141 tcgtattttg gtagatggtc aggagttatc ggaaaatcgc ttggcgatca aacgaaagat
    43201 tggctacgta gcagactcgc ctgacttatt tttacgttta acggccaatg aattttggga
    43261 attgatcgcc tcatcctatg atctgactag ctctgacttg gagactagtc tagctaggct
    43321 attgaacgtt tttgattttg ctgaaaatcg ttatcaggtt attgaaaccc tttctcatgg
    43381 aatgcgtcag aaagtttttg tcatcggggc actcttgtct gatcctgata tttgggtctt
    43441 ggacgaaccc ttgactggtt tggatcccca ggctgccttt gatttgaaac agatgatgaa
    43501 ggaacatgca caaaaaggga agacggtctt gttttcaact catgtcctag aggtagccga
    43561 gcaagtctgt aatcggattg ccattttgaa aaaggggcat ttgatctatt gtggtagtgt
    43621 agagaacttg aggaaagacc acccagacca gtctttggaa agtatctacc ttagtcttgc
    43681 tggtagaaaa gaggaggttg cggatgcgtc tcaaggtcat taaaaaatta gttgatatca
    43741 atatcctcta ttcatctcaa gaagctaatc tggctaatct acgaaagaag caggctaaga
    43801 atcctgggaa aaaagtaaat gtttccgcta gagtcctaag ttcttacatt ttttctagtc
    43861 tcttgatgct tttcatgttt ataaatatag cctttcgttt tccttttgag gaaatgccaa
    43921 gtttttttag tacgatggtt gctattttac tggtgctggc cttttcaact tctttcactg
    43981 cattttacaa tgtcttttat gagagtaagg acctggcctc ctataggccc tatgccttta
    44041 aagaatcaga gattataatg gccaaaggtc tgtctgtcct cttgccagct ctagctggaa
    44101 ttgtaccaat cctagcttat tttctggtcc tctacattag gctagcacct tctctgtggt
    44161 tgggtttgcc tttgatgcta ctgtccttga ccttattatt tgtctctgtt actttagtga
    44221 tggtagtggc agtacatttc ttggctcaga ctagggtctt cagaaagtat cggtctattt
    44281 tttcgaatgt gatgattggg ataggagttc tcataccttt aatatttatc ttctttcttc
    44341 agtcgacttt tggaagtatt gttgacaaag ttagagacat tccatttctc ctttatcctc
    44401 ttcatatctt ttacaaaata gcagtggagc ctttttcgac agaagcctta gtgggtctgc
    44461 tcgcttggat aggactaact ctcttcctgc tttatctgac caaaaagaag gtccttcctc
    44521 gtttttatga cgtgatcctg cttaatagtg aggataaggt caaaaaagaa cgtcgcagca
    44581 aggagagaat ttcaaccact aaaaagggat tttttcgcat ggttttacgc taccacctca
    44641 ccctcttggg acaggggact ggcgtggtta cagtgctttt tacaagtgct ttctttcctt
    44701 atctcatgat gatcggtctg atttccaaaa tccgagattc tcagatagtt ccagacattc
    44761 atcctccata ctggttaccc ttgtttttta tagctctctt tatagcagtt gtcaataaca
    44821 atatcaccag cctgcattca attgccttgt ccttggagag ggaaaatgtt gattttctta
    44881 agagtttacc ctttgacttt gctcgttatg tgaaagtgaa attttggatt atatatgctg
    44941 tccagtcctt tttaccagtt ctgactttgc ttggtctttc tctatatcta ggcttgccca
    45001 tcatttcgat gatttacctt attgtggcat ggatccttgc cagtgtcatc ctttcttgcc
    45061 accattactt taaggacgtt aaaaatctgt caaccaattg gagtagcatt acggacctgg
    45121 tgaatcgttc aaatggcata gtcgccatag ttttattgtt tatttatagt gcaatcctga
    45181 tggcccttgt aattgggagc atattcttgg ttcagtctct ctccactatc cttgccatca
    45241 gcttgggagt aggagctctt atcctcctgc ttgctcttgc tatttttggc tatcattatt
    45301 acctgtcacg catattggca gaaatagaaa aaagatgagc tggttggtcg cttgcttgat
    45361 aaattcaatt cgacaaacgc ttttatagtt tgttagaatg tagttgtaaa tgattaagat
    45421 tggtaatcaa aaatataggg aggaatgttt tgaataagaa atataacata gttctatttt
    45481 tgttgtttat agtttattta tttggatatt tttcaatttc aaaaacgtta attcctataa
    45541 tgtgtgtgtt ccaagtattc ttgatagaac atatatttag aattcgaaat agaatgatgc
    45601 agataggtga aattataatt att
//
LOCUS       DBERCA010000012        39497 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.012, whole genome shotgun sequence.
ACCESSION   DBERCA010000012 DBERCA010000000
VERSION     DBERCA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.012"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            314..1591
                     /locus_tag="ACL719_000749"
     CDS             314..1591
                     /locus_tag="ACL719_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGS0698755.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1667..3151
                     /gene="thrC"
                     /locus_tag="ACL719_000750"
     CDS             1667..3151
                     /gene="thrC"
                     /locus_tag="ACL719_000750"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGS0698756.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3212..4492
                     /locus_tag="ACL719_000751"
     CDS             3212..4492
                     /locus_tag="ACL719_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS0698757.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4496..5116
                     /locus_tag="ACL719_000752"
     CDS             4496..5116
                     /locus_tag="ACL719_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0698758.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5282..6394
                     /locus_tag="ACL719_000753"
     CDS             5282..6394
                     /locus_tag="ACL719_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS0698759.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6717..7142
                     /locus_tag="ACL719_000754"
     CDS             6717..7142
                     /locus_tag="ACL719_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS0698760.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7153..7554
                     /locus_tag="ACL719_000755"
     CDS             7153..7554
                     /locus_tag="ACL719_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGS0698761.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            7629..8273
                     /gene="pcp"
                     /locus_tag="ACL719_000756"
     CDS             7629..8273
                     /gene="pcp"
                     /locus_tag="ACL719_000756"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS0698762.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(8301..9158)
                     /locus_tag="ACL719_000757"
     CDS             complement(8301..9158)
                     /locus_tag="ACL719_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGS0698763.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFILDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9288..10430
                     /gene="tgt"
                     /locus_tag="ACL719_000758"
     CDS             9288..10430
                     /gene="tgt"
                     /locus_tag="ACL719_000758"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGS0698764.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10531..12348
                     /locus_tag="ACL719_000759"
     CDS             10531..12348
                     /locus_tag="ACL719_000759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS0698765.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHIILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12501..13652
                     /gene="nagA"
                     /locus_tag="ACL719_000760"
     CDS             12501..13652
                     /gene="nagA"
                     /locus_tag="ACL719_000760"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGS0698766.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13815..14873
                     /locus_tag="ACL719_000761"
     CDS             13815..14873
                     /locus_tag="ACL719_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGS0698767.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15024..15389
                     /locus_tag="ACL719_000762"
     CDS             15024..15389
                     /locus_tag="ACL719_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGS0698768.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15465..16406
                     /gene="comGA"
                     /locus_tag="ACL719_000763"
     CDS             15465..16406
                     /gene="comGA"
                     /locus_tag="ACL719_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGS0698769.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16354..17370
                     /gene="comGB"
                     /locus_tag="ACL719_000764"
     CDS             16354..17370
                     /gene="comGB"
                     /locus_tag="ACL719_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGS0698770.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17372..17698
                     /gene="comGC"
                     /locus_tag="ACL719_000765"
     CDS             17372..17698
                     /gene="comGC"
                     /locus_tag="ACL719_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HGS0698771.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVND"
     gene            17691..18095
                     /gene="comGD"
                     /locus_tag="ACL719_000766"
     CDS             17691..18095
                     /gene="comGD"
                     /locus_tag="ACL719_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGS0698772.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18058..18360
                     /gene="comGE"
                     /locus_tag="ACL719_000767"
     CDS             18058..18360
                     /gene="comGE"
                     /locus_tag="ACL719_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGS0698773.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18323..18784
                     /gene="comGF"
                     /locus_tag="ACL719_000768"
     CDS             18323..18784
                     /gene="comGF"
                     /locus_tag="ACL719_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGS0698774.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            18762..19175
                     /gene="comGG"
                     /locus_tag="ACL719_000769"
     CDS             18762..19175
                     /gene="comGG"
                     /locus_tag="ACL719_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGS0698775.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19312..19906
                     /locus_tag="ACL719_000770"
                     /pseudo
     CDS             19312..19906
                     /locus_tag="ACL719_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            19967..20920
                     /locus_tag="ACL719_000771"
     CDS             19967..20920
                     /locus_tag="ACL719_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0698776.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYTHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            20971..22161
                     /locus_tag="ACL719_000772"
     CDS             20971..22161
                     /locus_tag="ACL719_000772"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGS0698777.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFSCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22162..22293
                     /locus_tag="ACL719_000773"
     CDS             22162..22293
                     /locus_tag="ACL719_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698778.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22310..22681
                     /gene="rnpA"
                     /locus_tag="ACL719_000774"
     CDS             22310..22681
                     /gene="rnpA"
                     /locus_tag="ACL719_000774"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGS0698779.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22656..23480
                     /locus_tag="ACL719_000775"
     CDS             22656..23480
                     /locus_tag="ACL719_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS0698780.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23499..24485
                     /gene="jag"
                     /locus_tag="ACL719_000776"
     CDS             23499..24485
                     /gene="jag"
                     /locus_tag="ACL719_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGS0698781.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24536..25159
                     /locus_tag="ACL719_000777"
     CDS             24536..25159
                     /locus_tag="ACL719_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGS0698782.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25290..26747
                     /locus_tag="ACL719_000778"
     CDS             25290..26747
                     /locus_tag="ACL719_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0698783.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26770..27051
                     /locus_tag="ACL719_000779"
     CDS             26770..27051
                     /locus_tag="ACL719_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0698784.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27128..27613
                     /locus_tag="ACL719_000780"
     CDS             27128..27613
                     /locus_tag="ACL719_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0698785.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27630..28295
                     /locus_tag="ACL719_000781"
     CDS             27630..28295
                     /locus_tag="ACL719_000781"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGS0698786.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28299..29162
                     /locus_tag="ACL719_000782"
     CDS             28299..29162
                     /locus_tag="ACL719_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGS0698787.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29164..29868
                     /locus_tag="ACL719_000783"
     CDS             29164..29868
                     /locus_tag="ACL719_000783"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGS0698788.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKTWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30047..31720
                     /locus_tag="ACL719_000784"
     CDS             30047..31720
                     /locus_tag="ACL719_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0698789.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            31832..32923
                     /gene="ulaG"
                     /locus_tag="ACL719_000785"
     CDS             31832..32923
                     /gene="ulaG"
                     /locus_tag="ACL719_000785"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGS0698790.1"
                     /translation="MPNVKEITRELWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RINVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLTVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33037..35013
                     /gene="tkt"
                     /locus_tag="ACL719_000786"
     CDS             33037..35013
                     /gene="tkt"
                     /locus_tag="ACL719_000786"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS0698791.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAIEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35130..35429
                     /gene="yajC"
                     /locus_tag="ACL719_000787"
     CDS             35130..35429
                     /gene="yajC"
                     /locus_tag="ACL719_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS0698792.1"
                     /translation="MNPNYTFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35476..35904
                     /locus_tag="ACL719_000788"
     CDS             35476..35904
                     /locus_tag="ACL719_000788"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGS0698793.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            35906..36316
                     /locus_tag="ACL719_000789"
     CDS             35906..36316
                     /locus_tag="ACL719_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGS0698794.1"
                     /translation="MENPTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36617..39268
                     /gene="adhE"
                     /locus_tag="ACL719_000790"
                     /gene_synonym="adhC"
     CDS             36617..39268
                     /gene="adhE"
                     /locus_tag="ACL719_000790"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGS0698795.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12083 a         7019 c         8839 g        11556 t
ORIGIN      
        1 aaaaatttaa gtaaagaaat aaggcatcaa atccaagata ggaattccta aaaaattact
       61 aaaaactaaa ataattattg tttttatagt ataactaatt tcttaaaaca ttgaattaat
      121 ctgaattatg acgtaagtct gctaatttca ctgctctcaa aatccagtgt ccgcaaggac
      181 gtgccggttc gaccccggcc gccggtatag tatagtgtta ggaacgttgt tattcttcgt
      241 tcttttttta tattattttt ggtataatta tagttattca aattttattt agattaagaa
      301 agtgtagggt agtatgtctt gttctatcga tttattaaaa catcggtatt tgaaaaatat
      361 taaagaaaat cctgaattgt ttgtcggaat tgagttggag tatcctgttg caagtttaga
      421 aggggatgct acagatgttg aagttataaa ggatctattt cattatttag tttctacttt
      481 ggatctcacc gtagcaaagg tagatgattt tggcaatctg atccagttag tagatccgat
      541 aagtcaggat gctattttat ttgaagtttc ctatacaacg attgagtttg catttggtaa
      601 ggctgaaacg attcaagagg tcgaaaatcg tttcaataat tatatgaatg taattcagag
      661 aaagttagct gaatcaaatc atgctattgt tggctgtggt atccatccca actgggataa
      721 aaatgagaat tgtccagtgg cttatccacg ctatcagatg ttgatggatt atttgaattt
      781 gagtagaaat attattaaat cagatttaca tcatttccct gaatatggta cttttatctg
      841 tgggagccag gttcagctgg atatttcaaa aaccaactac ttacgggtga ttaatgcttt
      901 tactcaaatt gaagcggcta aggcttattt atttgcaaac tctgaatttt cgggtgcgga
      961 ttgggatacg aaaatttcaa gggatatttt ctgggaagaa tctatgcatg gtatctatcc
     1021 agagaatgtt ggggtcaatg ctagactcct taatgatgaa gctgattttt ttgactatct
     1081 aaatcattct gcgattttta ctgcggaacg tgatgggcag acctattatt tttatcctat
     1141 tcaggctggg gactatttgg ctacgcctga aatccaagca tttgctctga atggggatga
     1201 ggttattatt tccccccaag agaaggattt tgaaactcat cgtagttacc agtaccaaga
     1261 cttaacgact cgaggaacag ttgagtttcg tagtgtgtgt acacagccac ttgataggac
     1321 ttttgcttct gcagcttttc acttgggatt attggttaat ttagacaagt tagaagctta
     1381 cttagaaaca gcacctttct ttaaagtatt tggttatgat tacaagtctt taaggagaca
     1441 attttctaag aaaaatctta cagatgagga agaaactacg attattgaat tttccaaaga
     1501 cttactccta ctagctgagg agggactagt ggtgagaaat aaggaagaaa tgacctattt
     1561 acagcctttg agagaagaat tgagcctata atttctctta taaagggaga attttctgaa
     1621 aaatcatgat ataatggacg agactataga taaaggatag agagtaatga cattagttta
     1681 tcaatcaacg cgtgatgcca acaatacagt aactgctagc caagcaattt tgcaaggttt
     1741 ggcgacggac ggcggtttgt ttacaccggt tacttatcca aaggtagatt tggactttga
     1801 caaattgaaa gatacttctt accaggaagt tgctaagcta gttttgtcag catttttaga
     1861 tgactttaca gctgaggagt tggactactg tatcaacaat gcctacgata gcaaatttga
     1921 tactccagct attgcaccat tagtgaaatt agatgggcaa tacaatttgg aacttttcca
     1981 tggttcaacg attgccttta aggatatggc cttgtctatt ttgccatact ttatgacgac
     2041 tgctgctaag aaacatggtt tggagaacaa gattgttatc ttgacagcga catctggtga
     2101 cacggggaaa gctgctatgg cggggtttgc ggatgtgcct ggtactgaga ttatcgtctt
     2161 ttatccaaag gatggtgtca gcaagattca agagttacaa atgaccactc aaactggcga
     2221 caatactcat gttattgcta ttgatggtaa ctttgacgat gcgcaaacaa atgtgaagca
     2281 catgtttaac gacgtggctc ttcgtgaaaa attgactacc aacaagttgc aattttcatc
     2341 agctaactct atgaacattg gtcgtctggt gccacaaatt gtttattatg tttatgctta
     2401 cgctcaattg gttaagactg gtgaaattgt agctggtgaa aaggttaact tcacagtacc
     2461 aacaggaaac tttggaaata tcttggctgc cttttatgcc aaacaaattg gtttgccagt
     2521 tggtaaatta atctgtgctt caaatgacaa caatgttttg acagacttct ttaaaacacg
     2581 tgtctatgac aaaaaacgtg agtttaaggt aacaaccagc ccatctatgg atatcttggt
     2641 atcttcaaac ttggagcgct tgattttcca tcttttggga aataatgctg aaaagacaac
     2701 tgaacttatg aatgccttga acacgcaagg acaatataag ttgacagact ttgatgcaga
     2761 gattttggac ctctttgcag ctgaatatgc gactgaggaa gaaacggcag cagagatcaa
     2821 gcgtgtttgt gagttagatt cttatatcga ggaccctcat acagctgttg cttcagcagt
     2881 ttataaaaaa taccaatcgg ccactggaga tgtaactaag acagtgattg cttcaacagc
     2941 tagtccatac aagttcccag tagttgcagt agaagctgta actggaaaag caggtttaac
     3001 agactttgaa gccttggctc aattacatga aatctcaggc gttgcagtgc caccagcagt
     3061 tgatgggctt gaaatagctc caattcgtca caagacaaca gtggcagctg ctgacatgca
     3121 agcagcggtt gaggcttatt taggacttta agacagaggg agcaaactcg gttgggaaac
     3181 caactgagtt tcttttcatc aggaggagag attgtttaag aaaaataaag acattcttaa
     3241 tattgcattg ccagctatgg gtgaaaactt tttgcagatg ctaatgggaa tggtggacag
     3301 ttatttggtt gctcatttag gattgatagc tatttcaggg gtttcagtag ctggtaatat
     3361 tatcaccatt tatcaggcga ttttcatcgc tctgggagct gctatttcca gtgttatttc
     3421 aaaaagcata gggcagaaag accagtcgaa gttggcctat catgtgactg aggcgttgaa
     3481 gattacctta ctattaagtt tccttttagg atttttgtcc atcttcgctg ggaaagagat
     3541 gataggactt ttggggacgg agagggatgt agctgagagt ggtggactgt atctatcttt
     3601 ggtaggcgga tcgattgttc tcttaggttt aatgactagt ctaggagcct tgattcgtgc
     3661 aacgcataat ccacgtctgc ctctctatgt tagtttttta tccaatgcct tgaatattct
     3721 tttttcaagt ctagctattt ttgttctgga tatggggata gctggtgttg cttgggggac
     3781 aattgtgtct cgtttggttg gtcttgtgat tttgtggtca caattaaaac tgccttatgg
     3841 gaagccaact tttggtttag ataaggaact gttgaccttg gctttaccag cagctggaga
     3901 gcgacttatg atgagggctg gagatgtagt gatcattgcc ttggtcgttt cttttgggac
     3961 ggaggcagtt gctgggaatg caatcggaga agtcttgacc cagtttaact atatgcctgc
     4021 ctttggcgtc gctacggcaa cggtcatgct gttggcccga gcagttggag aggatgattg
     4081 gaaaagagtt gctagtttga gtaaacaaac cttttggctt tctctgttcc tcatgttgcc
     4141 cctgtccttt agtatatatg tcttgggtgt accattaact catctctata cgactgattc
     4201 tctagcggtg gaggctagtg ttctagtgac actgttttca ctacttggga cccctatgac
     4261 gacaggaaca gtcatctata cggcagtctg gcagggatta ggaaatgcac gcctcccttt
     4321 ttatgcgaca agtataggaa tgtggtgtat ccgcattggg acaggatatc tgatggggat
     4381 tgtgcttggt tggggcttgc ctggtatttg ggcagggtct ctcttggata atggttttcg
     4441 ctggttattt ctacgctatc gttaccagcg ctatatgagc ttgaaaggat aggaaatgca
     4501 aaaaacagct tttatttggg atttagacgg gactttattg gactcttacg aagcgatttt
     4561 atcagggatt gaggagactt ttgctcagtt ttctattcct tatgataagg agaaggtgag
     4621 agagtttatc ttcaagtatt cggtgcaaga tttgcttgtg cgggtggcag aagatagaaa
     4681 tctggatgtt gaggtgctaa atcaggtgcg tgcccagagt ctggctgaga agaatgctca
     4741 ggtagttttg atgccaggtg cgcgtgaggt gctagcttgg gcagacgaat caggaattca
     4801 gcagtttata tatactcata aggggaacaa cgcttttacc attctcaagg acttgggggt
     4861 ggaatcctat tttacagaga ttttaaccag tcagagtggc tttgtgcgga agccaagtcc
     4921 agaagcggct acctatctgc tagataagta tcagttgaat tctgataata cttattatat
     4981 aggggatcgg actctggatg tggaatttgc ccagaatagt gggattcaaa gcatcaactt
     5041 tttagagtct acttatgaag ggaatcacag gattcaagcg ttagcagata tttcccgtat
     5101 ttttgagact aagtgataaa aagattgtgt cagttttgtg acagagacct aacaaactat
     5161 ttcaagtaac ctagtttgtt acaaggaata gacagttctg ttaaataggc ccgagagggc
     5221 tttttttcta cattttttgt gttatgatag acaggtactc atttgaaagg aatttgaaag
     5281 aatgaagaaa agaatgttat tagcgtcaac agtagccttg tcatttgccc cagtattggc
     5341 aactcaagca gaagaagttc tttggactgc acgtagtgtt gagcaaatcc aaaacgattt
     5401 gactaaaacg gacaacaaaa caagttatac cgtacagtat ggtgatactt tgagcaccat
     5461 tgcagaagcc ttgggtgtag atgtcacagt gcttgcgaat ctgaacaaaa tcactaatat
     5521 ggacttgatt ttcccagaaa ctgttttgac aacgactgtc aatgaagcag aagaagtaac
     5581 agaagttgaa atccaaacac ctcaagcaga ctctagtgaa gaagtgacaa ctgcgacagc
     5641 agatttgacc actaatcaag tgaccgttga tgatcaaact gttcaggttg cagacctttc
     5701 tcaaccaatt gcagaagtta caaagacagt gattgcttct gaagaagtgg caccatctac
     5761 gggcacttct gtcccagagg agcaaacgac cgaaacaact cgcccagttg aagaagcaac
     5821 tcctcaggaa acgactccag ctgagaagca ggaaacacaa gcaagccctc aagctgcatc
     5881 agcagtggaa gtaactacaa caagttcaga agcaaaagaa gtagcatcat caaatggagc
     5941 tacagcagca gtttctactt atcaaccaga agagacgaaa gtaatttcaa caacttacga
     6001 ggctccagct gcgcccgatt atgctggact tgcagtagca aaatctgaaa atgcaggtct
     6061 tcaaccacaa acagctgcct ttaaagaaga aattgctaac ttgtttggca ttacatcctt
     6121 tagtggttat cgtccaggag acagtggaga tcacggaaaa ggtttggcta tcgactttat
     6181 ggtaccagaa cgttcagaat taggggataa gattgcggaa tatgctattc aaaatatggc
     6241 cagccgtggc attagttaca tcatctggaa acaacgtttc tatgctccat tcgatagcaa
     6301 atatgggcca gctaacactt ggaacccaat gccagaccgt ggtagtgtga cagaaaatca
     6361 ctatgatcac gttcacgttt caatgaatgg ataaacccga cttgataaca tcattttgac
     6421 gaatgagatc tagctttcgt gatggaaagc gattctcgtt cgttttttct ttgtcatact
     6481 cttcgaaaat ctcttcaaac cacgtcagtt ttatctgaaa cctcaaagct gtgctttgag
     6541 caacctgcga ctagcttcct agtttgcttt ttgattttca ttgagtatca atttgaatgg
     6601 aaaatggaaa gttatcatct tgtaatgagt taagcaacat tcttgcaatc tattttactt
     6661 tatatcacaa ttaattagtc aactattgat aaatcaataa aaagagaggg gaagaaatgc
     6721 tagagattca agatttactg tatcaactcc gcttgtctga gcaagcgagt acgcaattgt
     6781 ttgaaaaaag gcttgggatt agtttgacac ggtatcagat tttactgttt ttgctggagc
     6841 attctccttg taaccaaatg gcgattcagg agcgtttgaa aattgatcag gctgctttga
     6901 cacggcattt caagattttg gaaacggaag gtttggtgga gcgtcatcgt aatcctgaaa
     6961 atcagcggga agtgttggta gaggctgcga agtatgccaa ggagcagtta gtggtgaatc
     7021 cccctctgca acatatcaag gttaaggaag agatggaaag tatcttaaca gagtttgaga
     7081 gaacagaact cagccgttta ttaaataaat tggttttggg tattgaaaat atagaaattt
     7141 aaggagaaat agatgtcaat tattacaatt attttaacaa cgatcgttgc tttggagcat
     7201 ttttacattt tttatttgga aagtattgct acgcgatcag atgcgactag tcgtgtattt
     7261 aatatggaaa aggaagaatt ggctcatccg tcagtaagtt cattgttcaa aaatcaaggg
     7321 atttataagg ctctgctagg agtctttctc tggtatggca tttatttctc acagaattta
     7381 gaaattgtga ctatttttgt cttatttgtg attggtgctg cgacttacgg ctctttaaca
     7441 gcggataaaa aaattatttt gaagcaaggt ggatcagcta ttttggcctt gattagtatt
     7501 ttactcttta aaatacactt gaaggtcgat tctaatctcg ctaatccttt ttaatccaga
     7561 ataagggaaa tatgttatac ttgtttttaa gaaaaaagtc tcattgaatt ggttttgagg
     7621 agttagaaat gaaagtatta gtgacaggtt ttgagccctt tggaggggaa aagggcaatc
     7681 caactttgga ggccattaaa ggtttaccag ctgaaatcca tggtgctgag gtccgttggc
     7741 tagaggtgcc gacagttttt cacaaatctg ctcaagtatt ggaagaagag atgaatcgtt
     7801 atcaacctga ctttgtcctt tgtattgggc aagctggtgg aagaactagt gtgacacctg
     7861 aacgagtggc cattaatcaa gacgatgcac gcatttctga taacgaagat aatcaaccga
     7921 ttgaccgtcc cattcgccca gatggtgctt cggcctactt tagtagtttg ccgattaaag
     7981 cgatggttca agctataaaa aaagagggct taccggcctc tgtttccaat acggcaggga
     8041 cttttgtctg cagccatttg atgtatcagg ctctctattt ggtagaaaag aaatttccat
     8101 atgttaaggc aggttttatg catattcctt atatgatgga acaggtggtg aacagaccga
     8161 ctactccaac tatgagttta gtggatattc ggcgagggat agaagcagca atcggcgcta
     8221 tgatagaaca tggggatcag gaactcaagt tggtaggcgg agaaactcat tgatagaaaa
     8281 aagcttgagg ggaaaaacct tcaagctttt ggacgttttc gagccaatac tgctcggtaa
     8341 aacataattt tagtgcattg gatataaggt aggagtgaaa aactagcaat gccaaaggta
     8401 atccaattga ggaagtacca aggaagaagc tgtaaatcta ggataaagtg ctggaacttg
     8461 tagcccttca taaaggaacg gctagttttt aggattcgtc ttggtgggac ctgtcctagg
     8521 tctagactat aacagagaag aaattccacc tgtgaatagg cataatactg tggaatatag
     8581 aggatatttc ctacaatgat caagatgaga cttgcaagaa agtagagtcc aaagaccatg
     8641 aggaaacgct cggtttcaac tgatgagaga tctagatttg gaaactcagg atgtagggtg
     8701 acgaattttt tggctaaaaa gctactataa aagaggagat aaatcccaag taaattaggg
     8761 atactccata aaaagagata gaaacgtttg agaagtaggg tcaaaaaggt ttgagaaaag
     8821 cgctcctcat caaagagagc taggctgttt tttacagatg gctccgtttt agaatctttc
     8881 atgagtgtca gtgttgcata gacggaactg gtcaaaagaa tagtcccgat aaaggagact
     8941 agtagaggaa agaggtaggc ttgaagtatt tggccaagta tgctgaaaaa tggctgttct
     9001 aaaacagtcc cgtggatccg agataaggga ttaagaaaac tagataagat gaccagcata
     9061 ctgggaagga tatagaggag aaagagacgg ggggtgtcag cctgaaaatg ttttgactcc
     9121 tgacgaattg tttttaaatc aatttttgga tagttcattc tcttattata ccatagttct
     9181 tatacatagt tcgtgacagt tcctactttt tttgataaaa tcatacagtg tgtacttggg
     9241 cacactgtat gaactgggac tgtctttccc agcttcggag gtaaaaaatg tcagattcac
     9301 caatcaaata tcgtttgatt aagaaagaaa aacacacagg agctcgtcta ggagaaatca
     9361 tcactcccca cggtaccttt ccgacaccta tgtttatgcc agttgggaca caagccactg
     9421 tcaaaactca gtcacctgaa gaattgaagg agatgggttc gggaattatc ctatcaaaca
     9481 cttatcatct ctggcttcgc cctggagatg aactcattgc acgcgctggt ggtctccaca
     9541 agttcatgaa ttgggaccag cctatcttga cagatagtgg tggttttcag gtttattctt
     9601 tagcagatag ccgtaatatc acagaagaag gagtaacctt taaaaatcat ctaaatggtt
     9661 ctaagatgtt cctatcccca gaaaaagcca tctctattca gaataatctg ggttcagaca
     9721 tcatgatgtc ctttgatgaa tgtcctcagt tttatcaacc ttatgactac gttaagaaat
     9781 cgatagagcg taccagccgt tgggctgagc gtggtttgaa ggctcaccgt cgtccacatg
     9841 accaaggttt gtttggaatt gtgcaaggtg caggatttga agaccttcgc cgccaatcag
     9901 ctcatgatct tgtcagcatg gatttctcag gctactctat cggtggtttg gcagtgggag
     9961 aaacccatga agagatgaat gcggtcttgg actttacaac tcaactgctg cctgaaaata
    10021 aacctcgtta tctgatgggt gtgggagcgc cagatagctt gatcgatggg gtcattcgtg
    10081 gggtggatat gtttgactgt gtcttaccga ctcgaattgc tcgtaacggg acttgtatga
    10141 ccagtcaagg acgtttggtt gtgaaaaatg cccagtttgc tgaggacttt acgccactgg
    10201 atcctgagtg tgattgctac acatgtaata actatacacg cgcttacctt cgtcacctgc
    10261 tcaaggctga tgaaaccttt gggatccgct tgactagcta ccacaatctt tacttcttgc
    10321 ttaacctgat gaagcaagtg cgacaagcca tcatggatga caatctcttg gaattccgtg
    10381 agtattttgt ggaaaaatat ggctataata agtcaggacg taatttctaa aatggaattg
    10441 atataagcta aaaatcctaa gttttctctt aggatttttc ttcttttttt gatagaataa
    10501 agtgtacaat gaaaggaaga ataaactcgt atgcgcatta aatggttttc cttggttagg
    10561 attacaggtt tacttttggt actcttgtat cacttctttc agacgatctt tcctggagga
    10621 tttttcgggg tagatgtctt tttcacattt tcaggtttcc tgattacagc tctactcatt
    10681 gaagaatttt ctaaaaacca tgagattgat ttgataggat tttttagaag acgcttttat
    10741 cggattgtgc cacctgtggt tttgatggtc ttggtgacca tgccttttac tttcttggtt
    10801 cgccaagact atgttgctgg aattggtggc cagattgcga gcgtcttagg ctttatgacc
    10861 aacttctatg aactcctaac aggtgggagt tatgaatctc agttcattcc tcatttgttt
    10921 gttcataatt ggagtttggc agttgaggtt cactactata ttctttgggg attggcagtt
    10981 tggttcttat ccaaacaagc taaatcaaat ggtcagttga aggggatggt ctttctctta
    11041 tctgctgttg ccttcttgat cagtttcttc tccatgttta ttggtagttt tctagtgacc
    11101 tcttattcct ctgtttattt ctccagttta actcatgtct atccattctt tttgggaagt
    11161 atgttagcaa ctattgtagg cgttcgtcag acgacttccc tcgtcaagca gttggataaa
    11221 atctgggatt tacgaaagac tttggtagtt tttggaggag gctttggttt cctagttctt
    11281 ttgactttct ttgtcaaatt cacttatctt tttgcctatc ttatcggctt cttacttgcc
    11341 agtcttgcag ctcttgccat gattctggcg gcgcgtgtct tacatgaaaa gacacatcat
    11401 atacaggagc cgaagattat cagcttttta gcggatacta gctatgcggt ttatcttttc
    11461 cattggcctt tctatatcat tttctcacag ttgacatcaa atcttcttgc tgtattactg
    11521 actctgattt gttcttatgg ctttgccagt ctgtcatttt atgtattgga accttggatt
    11581 gcaggcaaga acacacctat tgtccaaacc cttcgtcccc tgccttatat tcacgcaatt
    11641 cttgcagcag gtacaggaat cttgaccatc attgtctgca cggtgacctt gttggcacca
    11701 caagtgggag cgtttgagac agacttgact gtcaatggct tgaagcaagc tgcaacaaat
    11761 attggccaga ccaaggtgat ggcagaacgg gcagatgcaa acagtttggg aattgctgat
    11821 ggcactatgt taattggtga ctcagtggct ttaagggcaa atacagcact acagacagct
    11881 cttcctggag cacagattaa cgcgcaggtc agcgtaacaa ccaagaccgc aaatgaaatc
    11941 atgctaaata atagccagaa taaattttta cctaagacgg tggtcattgc gactggggta
    12001 aataatcctg agaattacaa ggatgactgg gacagtatcg tgaaaaatct tcctaaggga
    12061 caccatataa ttttggtgac tccttatgag ggagataaga caaaagagac ctatgccatc
    12121 gttgagaagg ctgctgccta tatgagagaa ttggcagaga agacacctta cattacgata
    12181 gcagattgga atcaagttgc gaaagagcat ccagaaattt gggcaggaac agaccaggtt
    12241 catttcggga gtgagagtag cactatcgaa gcaggagcaa aattgtatgc agatacgatt
    12301 gccacagctt tgcagacagc tcaagacaag ccggttaaat caaaataact tgtattaaaa
    12361 aagagaagag ttggagaaat ccagctcttt taaaatatct ccaaaaaata ggtctatacc
    12421 atttacaaat aaaaaagaaa ggtttataat gtaattgaca taataaattg tagaatcaat
    12481 cttttaagga ggttaacatt atgcctaact atattaaagc ggatcagttt ttctacccac
    12541 acggagttcg tcgaggtggt tacttggaac ttgtggacgg caagtttggg aaacatgtag
    12601 agcagattcc tgaaggggct gaggtgattg actatacagg ttatagcatt gccccaggtc
    12661 ttgtggatac tcatattcat ggatatgcag gtgtagatgt gatggacaac aacattgaag
    12721 gtacattgca tactatgagt gaaggacttc ttagtaccgg tgttaccagt ttcttaccca
    12781 caactttaac agccacttat gagcaattgc ttgcagtcac tgaaaatctt ggaaaccatt
    12841 ataaagaagc aacaggtgct aagattcgtg ggatttatta tgaaggtcca tatttcacag
    12901 aaacttttaa gggggcacaa aatccaactt atatgagaga cccgggtgtt gaggagtttc
    12961 attcttggca aaaagcggca aatggcttgc ttaataaaat tgcccttgca ccagaacgtg
    13021 atggggtgga agactttgtt cgtacagtta cgggcgaagg tgtgacggtt gctcttggac
    13081 attcaaacgc gacttttgat gaagccaaaa aagcagtcga tgctggagcg agtgtttggg
    13141 tgcatgccta caatggaatg cgtgggttga ctcaccgtga attgggtatg gttggagcca
    13201 tgtaccaatt gccacatacc tatgcagagt tgatctgtga tggtcaccac gtagatccaa
    13261 aggcttgcga aattcttatc aaacaaaaag gaacagaaaa catcgctctt atcacagact
    13321 gtatgacagc tgggggattg gaagacggag attatatgtt gggagaattc ccagttgtcg
    13381 ttgcaaatgg aactgcacgc ctcaaatcga caggtaactt ggcaggttct atcctcaaac
    13441 tcaaagatgg tttgaagaat gtggtcgaat ggggaattgc gaatccgcat gaagcagtca
    13501 tgatggccag cttcaaccca gctaaatccg ttcacatcga tgacgtctgt ggccaaatcc
    13561 gtgaaggcta cgacgctgac ttcatcgtat tagataaaga tttggaattg gtagcaacct
    13621 acctagatgg cgtaaaacgt tatcaagcat aagagagaaa gcagaagtta gagactaact
    13681 tctgcttttt tatgcagggt actttactgg taaataaaaa gttaaaaaaa tcacaaaaaa
    13741 agcttgaaga aacaaagaaa aaggcttata attgaactat aaatattaca aataaaaaaa
    13801 cggaggagtg ctttatgaaa gcctatactt atgttaaacc aggacttgct tcttttgttg
    13861 atgtagacaa accagttatt cgcaagccaa cagacgctat tgtgcgtatt gtaaaaacca
    13921 ctatttgtgg aacagacctc catattatca aaggggatgt tcctacttgc caaagtggta
    13981 ccattcttgg ccacgaaggg attgggattg ttgaagaagt tggggaagga gtttccaact
    14041 tcaaaaaagg tgacaaggtc ttgatttctt gcgtctgtgc ctgtggtaaa tgctactact
    14101 gtaaaaaagg aatttatgct cactgtgaag acgaaggggg ctggattttc ggtcacttga
    14161 ttgatggtat gcaggctgaa tatctacgtg tccctcatgc agataatact ctttaccata
    14221 ctccagaaga cttgtcagat gaagctttgg ttatgctgtc agacattctg cctactggat
    14281 atgaaattgg tgtcttaaaa gggaaagtag aacctggttg cagcgtagcc attattggtt
    14341 caggtccagt tggattggct gctcttttaa cagcccaatt ctattcacca gctaaattga
    14401 ttatggtaga cctagacgat aaccgcttgg aaactgccct atcattcggt gcgactcata
    14461 aggttaattc ttcagaccct gaaaaagcca ttaaagaaat ttatgatttg acagatggtc
    14521 gtggtgtgga tgtcgctatc gaagctgttg gtattcctgc aacatttgat ttctgtcaaa
    14581 agattatcgg tgtagacgga acggttgcca actgtggtgt gcatggtaaa ccagttgaat
    14641 tcgatttaga taaactttgg attcgcaaca tcaatgtaac aactggtttg gtatctacaa
    14701 atacgactcc acaattgttg aaagcgcttg aaagtcataa gattgaacca gaaaaattgg
    14761 taactcacta tttcaaactc agtgaaattg aaaaagccta cgaagtcttc agtaaggcag
    14821 cagaccacca tgccattaag gtcattatcg aaaacgatat ctcagaagcc taagtagtaa
    14881 aaatattttt gtacataagt aaatagaaat tcagtcatcc atcagatggc tggatttttt
    14941 atcaaaaaat taagaaatga gcatatttct ttccttgtct ggcggaattg gttataatat
    15001 acggtacaaa ggaatgaatg aatatgtatc gtgttataga aatgtacgga gattttgaac
    15061 cgtggtggtt cttagaaggt tgggaagaag atattgtagc aagtagaaaa tttgaccagt
    15121 attatgatgc tctcaaatac tacaaaactt gctggtttag attggaacaa gaatcccctc
    15181 tttataaaag tagaagcgac ttgatgacca ttttttggga cccggaagac caacgctggt
    15241 gtgatgaatg tgatgagtat ttacaacaat accattcttt ggctcttttg caggatgagc
    15301 aggttatccc agacgaaaaa ctacgctcag gctatgaaaa acaaaccagt caggaaagga
    15361 atcgttcttg ccgtatgaaa ttaaaataga gaaaagtaac ttttttggag ttgctttttt
    15421 tatttttcta acgctttgcg aatagtatag gtgaggaggt aagtatggtt caagaaattg
    15481 cacaagaaat cattcgctcg gctcggaaaa aaggggcgca agacatttat tttgtcccca
    15541 agttagacgc ctatgagctt catatgaggg taggagacga gcgctgtaaa attggtagct
    15601 atgattttga aaagtttgca gccgttatca gtcactttaa gtttgtggcg ggtatgaatg
    15661 tgggagaaaa aagacgtagt caactgggtt cctgtgatta tgcctatgac cagaagatgg
    15721 cgtctctacg tttatctact gtaggcgatt atcgggggca tgagagtttg gttatccgtt
    15781 tgttgcacga tgaggagcag gacttgcatt tttggtttca ggatattgaa gaattaggca
    15841 agcagtacag gcaacgggga ctctatcttt ttgctggtcc ggttgggagt ggtaagacga
    15901 ccttgatgca tgaattgtcc aagtcactct ttaaaggaca gcaagttatg tccatcgaag
    15961 atcctgtcga aatcaagcag gacgacatgc ttcagttgca gttgaacgaa gcaatcggtc
    16021 taacctatga aaatctaatc aaactttcct tgcgtcatcg accagatctc ttgattatcg
    16081 gagaaattcg tgacatcgag acggcgcgtg cagtggtcag agctagtttg acaggtgcga
    16141 cagtcttttc aaccattcac gccaagagta tccgaggtgt ttatgagcgt ctgctggagt
    16201 tgggtgtgag tgaagaagaa ttggcagttg ttctgcaagg agtctgctac cagagattaa
    16261 tcgggggagg aggaatcgtt gactttgcaa acagagatta tcaagaacac caagcagcca
    16321 agtggaatga gcaaattgac cagcttctta aagatggaca tatcacaagt cttcaggctg
    16381 agacggaaaa aattagctac agctaagcaa aaaaatatca tcaccctatt taacaatctc
    16441 ttttctagcg gttttcatct ggtggagact atctcctttt tagataggag ttccttgttg
    16501 gacaagcagt gtgtgaccca gatgcgtaca ggcttgtctc aggggaaatc attctcagaa
    16561 atgatggaaa gtttgggatg ttcaagtacc attgtcactc agttatctct agctgaagtt
    16621 catggcaatc ttcacctgag tttgggaaag atagaagaat atctagacaa tctggccaag
    16681 gtcaagaaaa agttaattga agtagcgacc tatcctttga ttttgctggg ttttcttctc
    16741 ttaattatgc tggggctacg gaattacctg ctcccacaac tggatagtag caatattgcc
    16801 acccgaatta tcggtaatct gccacaaatt tttctaggca tggtagggct tgtttccgtg
    16861 cttgcccttt tagcactaac tttttataaa agaagttcta agatgagtgt cttttctatc
    16921 ttagcacgcc ttccctttat tggaatcttt gtgcagacct acttgacagc ctattatgca
    16981 cgtgaatggg ggaatatgat ttcacaggga atggagctga cgcagatttt tcaaatgatg
    17041 caggaacaag gttcccagct ctttaaagaa atcggtcaag atctggctca aaccctgaaa
    17101 aatggccgtg aattttctca gacgatagga acctatcctt tctttaggaa ggaattgagt
    17161 ctcatcatag agtatgggga agttaagtcc aagctgggta gtgagttgga aatctatgct
    17221 gaaaaaactt gggaagcctt ttttacccga gtcaaccgca ccatgaattt ggtgcagcca
    17281 ctggttttta tctttgtggc actgattatc gttttacttt atgcggcaat gctcatgccc
    17341 atgtatcaaa atatggaggt aaatttttaa aatgaaaaaa atgatgacat tcttgaaaaa
    17401 agctaaggtt aaagctttta cattggtgga gatgttggtg gtcttgctga ttatcagcgt
    17461 gcttttcttg ctctttgtac ctaatctgac caagcaaaaa gaagcagtca atgacaaagg
    17521 aaaagcagct gttgttaagg tggtggaaag ccaggcagaa ctttatagct tggaaaagaa
    17581 tgaagatgct agcctaagca agttaaaaga agatggacgc atcacggaag aacaggctaa
    17641 agcttataaa gaatacaatg ataaaaatgt gggagcaaat cgtaaagtca atgattaagg
    17701 cctttaccat gctggaaagt ctcttggttt tgggtcttgt gagtatcctt gccttgggct
    17761 tgtccggctc tgttcagtcc acttttgcgg cggtagagga acagattttc tttatggagt
    17821 ttgaagaact ctatcgggaa acccaaaaac gcagtgtagc cagtcagcaa aagactaatc
    17881 taaatttaga tgggcagacg cttagcaatg gcagtcaaaa gttgacagtt cctaaaggaa
    17941 ttcaggcacc atcaggccaa agtattacat ttgaccgagc tgggggcaat tcgtccctgg
    18001 ctaaggttga atttcagacc agtaaaggag cgattcgcta tcaattatat ctaggaaatg
    18061 gaaaaattaa acgcattaag gaaacaaaaa attagggcag tgattttact ggaagcagta
    18121 gtcgctctag ctatctttgc cagcattgcg accctccttt tgggacaaat tcaaaaaaat
    18181 aggcaagagg aagcaaaaat cttgcaaaag gaagaagtct tgagggtagc taagatggcc
    18241 ctgcagacgg ggcaaaatca ggtaagcatc aacggagttg agattcaggt attttctagt
    18301 gaaaaaggat tggaggtcta ccatggttca gaacagttgt tggcaatcaa agagccataa
    18361 ggtcaaggct tttaccttgt tagaatccct acttgccctc attgtcatca gtgggggatt
    18421 actccttttt caaactatga gtcagctcct catttcagaa gttcgctacc agcaacaaag
    18481 cgagcaaaag gagtggctct tgtttgtgga ccaacttgag gcagaattag accgttcgca
    18541 gttcgaaaaa gtagaaggca atcgcctata catgaagcaa gatggcaagg acatcgccat
    18601 cggtaagtca aagtcagacg attttcgtaa aacgaatgct cgtggtcgag gttatcagcc
    18661 tatggtttat ggcctcaaat cagctcagat tacagaggac aatcaactgg ttcattttcg
    18721 tttccagttt caaaaaggct tagaaaggga gttcatctat cgtgtggaaa aagaaaaaag
    18781 ttaaggcagg tgttctcctc tacgcagtca ccatagcagc catctttagt cttttgttgc
    18841 aattttattt gaaccgacaa gtcgcccact atcaagacta tgctttgaat aaagaaaaat
    18901 tggttgcttt tgctatggct aaacgaacca aagataaggt tgagcaagaa agtggggaac
    18961 aggtttttaa tctaggtcag gtaagctatc aaaacaagaa aactggctta gtgacgaggg
    19021 ttcgtacgga taagagccaa tatgagtttc tgtttccttc agtcaaaatc aaagaagaga
    19081 aaagagataa aaaggaagag gtagcgaccg attcaagcga aaaagtggag aagaaaaaat
    19141 cagaagagaa gcctgaaaag aaagagaatt cctagtcaat tcaactataa tgcgttgaat
    19201 ccagaatagt ccactgtagt ttctagaaaa ttgctggaaa tggatgttaa gctccaattc
    19261 atttgtttat atcttatttc agtttactat actttgtgct aaattaaaga tatgaaacat
    19321 gattttaacc acaaagcaga aactttcgat ttccctaaaa atatcttcct cgcaaacttg
    19381 gtatgtcaag cagccgagaa acagattgat cttctatcat acaaagaaat tttagatttc
    19441 ggtggtggca cgggtctatt agccttgccc ctaaccccta gccaagcagg ctaagtcagt
    19501 cactcttgta gacatttctg agaaaatgtt ggagcaagct cgtttgaaag tggagcagca
    19561 agcaatcaag aatatccagt ttttggagca agatttaccg aaaaatccct tggagaaaga
    19621 gtttgattgc cttgctgtta gtcgggttct tcatcatatg cctgatttgg atgcggctct
    19681 ctcactgttt catcaacatt tgaaggaaga tgggaaactc atcattgctg attttaccaa
    19741 gacagaagct aatcatcatg gatttgattt agctgaactg gaaaacaagc taattgagca
    19801 tggtttttca tctgtgcata gtcagattct ctatagcgct gaagacctgt ttcaaggaaa
    19861 tcactcagaa ttctttttaa tagtagccca aaaatcactc gcctagtcag ggagtgattt
    19921 ttctataaga atggaaaaaa gaagggaaat ttgataagat aggaatatgg attttgaaaa
    19981 aattgaacaa gcttatacct atttactaga gaatgtccaa gtcatccaaa gtgatttggc
    20041 gaccaacttt tatgacgcct tggtggagca aaacagcatc tatctggatg gtgagactga
    20101 gctagaacag gtcaaagaca acaatcagac ccttaagcgt ttagcactac gcaaagaaga
    20161 atggctcaag acctaccagt ttctcttgat gaaggctggg caaacagaac ccttgcaggc
    20221 caatcaccag tttacaccgg atgctattgc tttacttttg gtgtttattg tggaagagct
    20281 gtttaaagag gaggaaatta ctatcctcga aatgggttct gggatgggaa ttttgggcgc
    20341 tactttcttg acctcgctta ctaaaaaggt ggattatttg ggaatggaag tggatgattt
    20401 gctgattgat ttggcagcta gcatggcaga tgtaattggt ttgcaggctg gctttgtcca
    20461 aggagatgcc gttcgtccac aaatgctcaa agaaagtgat gtggtcatca gcgacttgcc
    20521 tgttggctat tatcctgatg atgccgttgc gtcgcgccat caagttgctt ctagtcaaga
    20581 acatacttac acccatcact tgctcatgga acaaggactt aagtacctca agtcagacgg
    20641 atacgctatt tttctagctc cgagtgattt gttgaccagt cctcaaagta atttgttgaa
    20701 agtctggttg aaagaggagg caagtctggt tgctatgatt agtttacctg aaaatctctt
    20761 tgctaatgct aaacaatcta agactatttt tatcctacag aagaaaagtg aaatagcagt
    20821 agagcctttt gtttatccac ttgctagctt gcaagatgca agtgttttaa tgaaatttaa
    20881 agaaaatttt caaaaatgga ctcaaggtac tgaaatataa aatagatttt gttataatag
    20941 ttgaaaacgc ttaaaaaggg gtatcatgtt atgacaaaaa caattgcaat caatgcagga
    21001 agttcaagtt tgaaatggca attatactta atgccagaag aaaaagtatt ggcgaaaggt
    21061 ttgattgaac gtatcggttt gaaagattca atttcaactg taaaatttga cggccgttct
    21121 gaacaacaaa ttttggatat tgaaaatcat acacaagctg ttaaaatttt attggatgac
    21181 ttgattcgtt tcgatattat caaggcttat gacgagatta caggtgttgg acatcgtgtt
    21241 gttgctggtg gagaatattt caaagaatca acagttgttg agggagatgt tttagaaaaa
    21301 gttgaagagt tgagtttgtt ggctcctcta cacaacccgg caaatgcagc aggtgttcgt
    21361 gccttcaagg aattgttgcc agacattacc agtgtagttg tttttgatac ttccttccac
    21421 acaagtatgc cagagaaagc ttatcgctac cctctaccaa caaaatatta cacagaaaac
    21481 aaggttcgta aatacggtgc tcatggtaca agtcaccagt ttgtagcagg agaagctgca
    21541 aaactcttgg gacgtccatt agaagacttg aagttaatta cctgtcatat tggtaacgga
    21601 ggctcaatta cagctgtgaa agccggcaaa tctgtagaca cttctatggg gttcactcct
    21661 cttggtggta ttatgatggg aacgcgtaca ggggatattg atccagctat cattccttat
    21721 ttaatgcaat atacagagga ttttaacaca ccagaagata tcagtcgtgt tcttaaccgt
    21781 gaatcaggtc ttttgggagt ttctgctaat tctagcgata tgcgcgatat agaagcagct
    21841 gtagcagaag ggaatcacga ggctagcttg gcttatgaaa tgtatgttga ccgtatccaa
    21901 aaacatatcg gtcagtacct tgcagtgcta aatggagcag atgccattgt tttcacagca
    21961 ggtgtcggtg aaaatgcaga gagtttccgt cgtgatgtaa tctcagggat ttcgtggttt
    22021 agttgtgatg ttgatgatga aaagaatgtc tttggcgtta caggagacat ctcaacagag
    22081 gcagctaaaa tccgtgtctt ggttattcca acagatgaag aattagtcat tgcccgtgac
    22141 gttgaacgct tgaaaaaata agtgaaacta aaaaaatatt caatacaagg agttgggaaa
    22201 gttatttttc cagcttcttt ttctgatgaa attgtccaaa accttgctat gattggcttt
    22261 tttgaaaaat atggtataat agtagtaatt taatagatgg agttgagttt tgaagaaaaa
    22321 ctttcgtgta aaaagagaga aagattttaa ggcgattttc aaggagggga caagttttgc
    22381 taatcgcaaa tttgtggtct accaattaga aaaccagaaa aaccattttc gagtaggtct
    22441 atcagttagc aaaaaactgg ggaatgccgt cactagaaat caaattaagc gacggattcg
    22501 gcatattatc cagaatgcaa aagggagtct ggtagaagat gtcgactttg ttgtcattgc
    22561 tcgaaaagga gtcgaaacct tgggatacgc agagatggag aaaaatctac tccatgtatt
    22621 aaaattatca aagatttacc gggaaggaaa tgggagtgaa aaagaaacta aagttgacta
    22681 gtttgctagg actgtctctg ttaatcatga cagcctgtgc gactaatggg gtaactagcg
    22741 atattacagc cgaatcggct gatttttgga gtaaattggt ttacttcttt gcggaaatta
    22801 ttcgcttttt atcgtttgat attagtatcg gagtggggat tattctcttt acggtcttga
    22861 ttcgtacagt cctcttgcca gtctttcagg tgcaaatggt ggcttctagg aaaatgcagg
    22921 aagctcagcc acgcattaag gcgcttcgag aacaatatcc aggtcgagat atggaaagca
    22981 gaaccaaact agagcaggaa atgcgtaaag tatttaaaga aatgggtgtc agacagtcag
    23041 actctctttg gccgattttg attcagatgc cggttatttt ggccctgttc caagccctat
    23101 caagagttga ctttttaaag acaggtcatt tcttatggat taaccttggt agtgtggata
    23161 caacccttgt tcttccgatt ttagcagcag tattcacctt tttaagtact tggttgtcca
    23221 acaaagcttt gtctgagcga aatggcgcta cgactgcgat gatgtatggg attccagtct
    23281 tgatttttat ctttgcagtt tatgcgccag gtggagtcgc cctatactgg acagtgtcta
    23341 atgcttatca agtcttgcaa acctatttct tgaataatcc attcaagatt atcgcagagc
    23401 gcgaggccgt agtacaggca caaaaagatt tggaaaatag aaaaagaaaa gccaagaaaa
    23461 aggctcagaa aacgaaataa ataaggagga atctggtaat ggtagtattt acaggttcaa
    23521 ctgttgaaga agcaatccag aaaggattga aagaattaga tattccaaga atgaaggctc
    23581 atatcaaagt catttctagg gagaaaaaag gctttcttgg tctatttggt aaaaaaccag
    23641 cccaagtgga tattgaagcg attagtgaaa cgactgttgt caaagcaaat caacaggtag
    23701 taaaaggcgt tccgaaaaaa atcaatgatt tgaacgagcc tgtgaagacg gttagtgaag
    23761 aaaccgttga ccttggtcat gtggttgatg ctattaaaaa aatagaggaa gaaggtcaag
    23821 gtatttctga tgaagtcaag gctgaaatct taaaacatga aagacatgcc agcactatct
    23881 tagaagaaac tggtcacatt gagattttaa atgaacttca aatcgaggaa gcgatgaggg
    23941 aagaagcagg cgctgatgac cttgaaactg agcaagatca aactgaaaat caagacttga
    24001 aagagatggg cttgaaggtc gagcaaagtt atgatattgc ccaggtggct acggatgtga
    24061 ctgcttatgt tcaaacgatt attgatgaca tggatgttga ggctacactt tcaaatgatt
    24121 ataaccgtcg tagcatcaat ttgcaaattg atactaacga accaggtcgt attatcggct
    24181 accatggtaa agtcttgaag gccttgcaac tgttggctca aaattatctt tacaactgct
    24241 attcaagaac cttctatatc actattaatg tcaatgatta tgttgaacac cgtgcagaag
    24301 tcttgcagac ctatgcgcaa aaattggcga ttcgtgtttt agaagaaggg cgcagtcatc
    24361 aaacagatcc aatgtcgaat agtgaacgca agattatcca ccgtattatt tcacgtatgg
    24421 atggcgtgac cagttactct gaaggtgatg agccaaatcg ctatgtcgtt gtagatacag
    24481 aataagtaaa atcagggttg acctgatttt ttgctagcta gaggaggtta aactgatgtt
    24541 gaataagata agagactatc tagacttttc tggtttgcag taccgtagtc ctgataaagc
    24601 gggagcagag cgagagaaga tgctggcgtt ccgccacaaa ggacaagagg cccgaaaggc
    24661 ttttacagaa ctggccaaag cctttcaagc aagccatcca gaatggcaac tccaacagac
    24721 tagtcagtgg atgaatcagg cccagcgttt gagaccacat ttttgggttt atctacagag
    24781 agacggacaa gtgacagaac ctatgatggc cttacgtttg tatgggacat ctactgactt
    24841 tggaatttct ttggaagtca gtttcatcga acgtaagaag gatgagcaaa cactgggcaa
    24901 gcagaccaaa gttttagaca ttccaaccgt taaagggatt tattatctaa cctactctaa
    24961 tggtcaaagt caacggtggg aggcgaatga agaaaagcgt cgtactttac gcgagaaggt
    25021 gagaagtcaa gaagttcgaa aagttttagt gaaggtagat gttcctatga cagaaaattc
    25081 gtctgaagaa gaaatcgtag aaggcttatt gaagtcttat tctaaaattc ttccctatta
    25141 tctagctacg agaaaataag ataatttgta aaacatcata aatcatacag tccaagagtg
    25201 aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct ttcgcattat ataataaact
    25261 tacaaaaaca attcaaaagg agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt
    25321 tttctagcaa tattttgcag aatcccgcat ttttcgtagg tttattggtg ttgataggat
    25381 atgcactttt gaaaaaacct gcccatgacg ttttttcagg gtttgttaaa gcaacagtag
    25441 ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac aacctttcgt ccaatcttag
    25501 cagctcttaa ctacaaattc caaattggtg cagcggttat cgacccttac tttggacttg
    25561 ctgcagcaaa caacaaaatt gcagcagagt ttccagattt tgttggaact gcaactacag
    25621 ctctattgat tggttttgga ataaatatct tgctcgtagc tcttcgaaag attacgaagg
    25681 taagaaccct ctttattact ggtcacatca tggtacaaca agctgcaaca gtatctctta
    25741 tggttctatt cttagtacca caattgcgca atgcttacgg tacagcagcg attggtatca
    25801 tctgtggact ttactgggca gttagttcaa atatgactgt tgaggcaact caacgcttga
    25861 ctggtggtgg cggatttgcg attggtcacc aacagcaatt tgcaatctgg tttgtagata
    25921 aagtagcagg acgctttggt aagaaagaag aaagtttaga caatcttaaa ttacctaagt
    25981 tcctctcaat cttccacgat acagttgttg catctgctac cttgatgctc gtattcttcg
    26041 gagccattct tttaatcttg ggtccagaca ttatgtctaa taaagaagtc atcacttcag
    26101 gaactctatt caatcctgct aaacaagatt tctttatgta cattatccaa acagccttta
    26161 ccttctcagt ttacttgttc gttttgatgc aaggtgtccg aatgttcgta tctgagttga
    26221 caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg ttcattccca gcggttgacg
    26281 ttgcagcttc ttatggattt ggttctccaa atgctgtcct gtcaggattt atctttggtt
    26341 tgattggtca attgattaca attgttttgc tcatcgtctt taaaaatccg attcttatta
    26401 ttacaggatt tgtaccagtg ttctttgaca atgcagccat tgcggtctac gctgataaac
    26461 gcggcggatg gaaagcggct gttatccttt cctttatatc aggtgtcctt caagttgctc
    26521 taggagctct ttgtgtggcc cttctcgatt tggcatctta tggtggctac catggaaata
    26581 tcgactttga attcccatgg cttggatttg gatatatctt caaatacctt ggtattgttg
    26641 gttatgtact tgtgtgtctc ttcttgcttg ttattcctca acttcaattt gccaaagcaa
    26701 aagataaaga gaaatattac aacggtgaag ttcaagaaga agcttagtat ctagaaaagg
    26761 agaaataaaa tggttaaagt attagcagcg tgcggaaatg gaatgggttc atcaatggtt
    26821 atcaagatga aggttgaaaa tgctctccgt aagcttaatc aaacagattt tacagtcaat
    26881 tcatgcagtg tcggtgaagc taaaggttta gcagtaggat atgacatcgt aatcgcttct
    26941 cttcatttga ttcaagaatt ggaagggcga actaatggga agttaattgg gcttgataac
    27001 ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag cactacagta aaaggttgga
    27061 gggggctgga cagaaactga gagttatcgt ttctgtcctt ctccctcttt aaataaagga
    27121 ggcagatatg aatttaaaac aagctttaat tgacaacgac tcgatccgac taggtttaga
    27181 ggctaacgat tggaaagaag cagtcaaggt agcagtagat cccttgattg aaagtggggc
    27241 aattttgcca gagtattacg atgctatcat tgaatcgact gaagagtatg ggccttacta
    27301 tatcttgatg ccaggtatgg ctatgcccca cgctagacct gaagctggtg tgcaaagtga
    27361 tgccttttca ttgattacct tacaaaatcc tgttgtattt tcagatggga aagaggtatc
    27421 tgttttgttg gcactagcag caacaagttc aaaaattcac acaagtgtag ccattccaca
    27481 aattattgcc ctgtttgaat tagaagattc tattgcacgt ttacaggctt gccagactaa
    27541 agaagatgtc ttggctatga ttgaagaatc taaggatagc ccttatctcg aaggattgga
    27601 tttggaaagt tagaaagagg aataaagaaa tgacaaaaag aatacctaat ttacaagttg
    27661 cattagacca ttcagacttg caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag
    27721 tagatattat cgaagctgga actgtttgct tgcttcaagt tggaagtgaa ctggctgaag
    27781 tcttgcgtag ccttttccca gataagatta ttgtggcaga cacaaaatgt gctgatgctg
    27841 gtggaacagt tgctaaaaat aatgcggttc gtggagcaga ctggatgact tgtatctgtt
    27901 gtgcaaccat ccctactatg gaagcagctc taaaggctat caagactgaa cgaggagaac
    27961 gaggcgaaat ccagatcgag ctttatggcg attggacttt tgaacaagct cagctttggc
    28021 tagatgcagg tatctcacaa gctatttatc accaatctcg tgatgctctt cttgctggtg
    28081 aaacttgggg tgaaaaagac cttaataagg ttaaaaaact cattgacatg ggcttccgtg
    28141 tatctgtaac aggtggtcta gatgtagata ctctcaaact ctttgaaggt gttgatgtct
    28201 ttacctttat cgcaggtcgt ggaattacag aggctgcgga tccagcagga gcagcgcgtg
    28261 ccttcaagga tgaaatcaaa cgaatttggg ggtaaatcat ggtacgtcca attggaattt
    28321 atgaaaaggc aaccccaaca cactttactt ggctagaacg tttaaatttt gccaaggagt
    28381 taggctttga ttttgtcgag atgtctattg acgaacgtga cgagcgttta gcaagacttg
    28441 actggagtaa ggaagaacgc ttggaagttg tcaaagaaat ctatgaaact ggtgttcgta
    28501 ttccttctat ctgtttttca ggccatcgtc gctacccatt gggatcaaaa gatccagttc
    28561 tagaggaaaa atctctagaa ctcatgaaaa aatgtatcga attagctcaa gacttgggag
    28621 ttcgtacgat tcaattagct ggttacgatg tttactatga ggaaaagtca ccccagacac
    28681 gccaacgttt tatcaaaaat ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg
    28741 tacttgctat tgaaattatg gatgatcctt tcatcagtag catcgaaaaa tatttggcta
    28801 tagaaaaaga gattgactct cccttcctct ttgtatatcc agatattggt aatgtgtctg
    28861 catggcataa tgatatctat agtgagtttt atcttggtca tcatgccatc gcagctctcc
    28921 atctcaagga tacttatgca gtgacagaaa gttcaaaggg ccagttccga gatgtacctt
    28981 tcgggcaagg ttgtgtcaaa tgggaagaag ctttcgatat tttaaaggaa accaattata
    29041 atggaccttt cctaatcgaa atgtggtctg aaaattgtga aacagtagaa gaaacacgcg
    29101 cagccattca agaggcgcaa gcttttctct atccactcat taagaaagca ggtttgatgt
    29161 aagatgaatc aagtaatcaa tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg
    29221 ccaaaacatg gacttgtcaa atttacctgg gggaatgtat ctgaagtcaa tcgcgaactc
    29281 ggtgtcattg ttatcaaacc atcaggcgtg gattatgacg aattgacacc tgaaaacatg
    29341 gtagtgactg atctagatgg taagatccta gaaggggatt taagaccatc ttccgacctc
    29401 ccaactcatg tgcaattata taagacttgg tcagaaattg gtagtgtggt tcacacccat
    29461 tcgacagaag ctgttggttg ggctcaggca ggtcgtgata ttcctttcta cggaacaacc
    29521 catgcagatt atttctacgg ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta
    29581 gaagtggcct atgaaaaaga tactggcctg gttatcgtag aagagtttga acatcgcgga
    29641 cttaacccgg ttgaagtacc aggaattgtt gtacgcaatc acggtccatt cacctggggc
    29701 aaaaatccag agaatgctgt ttatcactct gtcgtactag aggaagtatc aaagatgaat
    29761 cgctttacag aacaaatcaa tccaagagtt ggacctgctc cccagtacat actagaaaaa
    29821 cactaccaac gtaaacatgg accaaatgct tattatggtc aaaagtaaga acgatgaagg
    29881 aggagaaaaa gataaattta gctcctcttt ttacatttga tttttattga gagtaaagtt
    29941 ggagttgaag taattttgaa agatttttta gaaatagcgc ttgatatata tatggtaaaa
    30001 taaaaagaat tgctgtgata tcaatagatt tgggggattt tttaatatgg tactggataa
    30061 ggcaagttgt gatttgcttc aatatttgat ggatcaagaa acgtccaaaa cgattatggc
    30121 gatttcgaaa gatttgaaag agtcaagaag gaaaatttat tatcacattg acaaaatcaa
    30181 tgctgctctg gttgacgagg cgcttcacat cattagtatt ccacgaattg gtattcactt
    30241 aacggaagag cagagagatg cttgttgtaa actattatcg gaagtagatt cgtacgatta
    30301 tatcatgagt gcgcatgaac gtatgatgat aatgttacta tggataggta tttctaaaga
    30361 acgtattacg attgaaaaat tgatagagtt aacagaggta tctaggaata ctgttctcaa
    30421 tgatttgaat agtattcgtt atcaactaac tttggaacaa tatcaggtga tcttgcaagt
    30481 gagcaagtca cagggatacc accttcatgc ccaccctctt aataaaattc agtatcttca
    30541 atcgcttcta tatcatattt ttatggaaga aaatgccact tttgtatcta ttttagaaga
    30601 taagatgaaa gagaggttag atgatgagtg tttgctttct gttgaaatga accaattttt
    30661 taaggaacag gttcctttag ttgaacaaga tttagggaag aaaataaacc atcatgaaat
    30721 aacttttatg ttgcaggttc taccttattt gctgttaagc tgtcataatg ttgaacagta
    30781 tcaagaaaga catcaggata tagagcaaga attttctttg ataagaaaaa gaatagagta
    30841 tcaggtgtct aagaaattag gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg
    30901 acttgaagtt tctcttgtag ctgttctcct cctctcctat cgtaaagatt tggatattca
    30961 tgcagaaagt gatgattttc ggcaattaaa acttgcttta gaagaattta tctggtattt
    31021 tgaatcacaa atccgaatgg agattgagaa caaggatgat ttgttacgaa atttgatgat
    31081 ccactgtaaa gccttgttat ttagaaagac ttacggtatt ttttctaaaa atcctctaac
    31141 aaaacaaatt cgatccaagt atggagaatt atttttagtc actagaaaat ctgcggaaat
    31201 tttagaagga gcatggttta ttcggctaac agacgatgat attgcctatt tgacgattca
    31261 tattggagga tttttaaagt atacaccatc gtctcaaaaa aatatgaaaa aagtttatct
    31321 cgtttgtgat gaaggtgttg cggtttcgag acttttgctg aaacaatgca aactttattt
    31381 tccaaatgag caaattgaca ctgtatttac aacagaacaa tttaagagtg tggaagatat
    31441 tgcacaagtt gatgtagtga ttactactaa tgatgatttg gatagcagat ttccgatttt
    31501 aagggttaat cctatccttg aagcagaaga tattttgaaa atgctagact atcttaaaca
    31561 caatatattt cgtaatgaga gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc
    31621 ttatattgta gacagcaagt tggctagtaa gttccaagaa gaggttcaaa cacttataaa
    31681 tcaagaaata gtagttcaag cttttttgga agatatttga aggacagtcc aatgatgaac
    31741 acaaacctgt gtttttcttg gtctttttta gtgttttgaa gggtggtata ctaatctcaa
    31801 agataacaat tatatccaaa ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga
    31861 gttatggatt ttagccactt tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga
    31921 agaagtcgta cctgaaggca actttgccat gtggtggcta ggcaactgtg gtacttggat
    31981 taagacacca gctggtgcta acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac
    32041 caaaaaagtg aaagatatgg ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa
    32101 gttgcaacca aacttgcgtg ttcagccaat ggttatcgat ccatttgcta tcaacgaact
    32161 agactattac ttagtttcac acttccacag tgatcatatc gacccataca cagctgcagc
    32221 aattctcaac aatcctaagt tagagcatgt taagtttatc ggtccttacc actgtggacg
    32281 aatctgggaa ggatggggtg ttccaaaaga acgtatcaac gttgttaaac caggtgacac
    32341 tatcgaatta aaagatatga agattcatgc agtagaatca tttgaccgta cttgcttggt
    32401 aactctccca gtgaacggtg ctgatgagac aggcggtgaa cttgctggct tgactgttac
    32461 agatgaagaa atggctcaaa aggctgttaa ctatatcttt gaaacaccag gtggaaccat
    32521 ctatcatggt gcagattctc acttctcaaa ctattttgca aaacatggta aagactttaa
    32581 aattgatgtt gctttgaata actatggtga aaatccggta ggtatccaag acaaaatgac
    32641 atctatcgac cttcttcgta tggcagaaaa tctgcgtacc aaagtcatta tcccagttca
    32701 ctatgatatc tggtctaact tcatggcttc tactaatgag attctagaac tttggaaaat
    32761 gcgaaaagat cgcttgcaat acgatttcca tccatttatc tgggaagttg gcggtaagta
    32821 cacttatcct caagatcaac acttagtaga ataccatcat ccacgtggtt ttgatgattg
    32881 ttttgaacaa gactctaaca ttcaatttaa agctttgcta taatataaaa atattctttg
    32941 gaggttatcc gtatgttgca tgatacggat aattttcata taatagtaaa atagaatgtg
    33001 tgattcaata atcacctcaa atagaaagga aattctatgt caaatctatc tgttaatgca
    33061 attcgttttc taggtattga cgccattaat aaagccaact caggtcatcc aggtgtggtt
    33121 atgggagcgg ctccgatggc ttacagcctc tttacaaaac aacttcatat caatccagct
    33181 caaccaaact ggattaaccg cgaccgcttt attctttcag caggtcatgg ttcaatgctc
    33241 ctttatgctc ttcttcacct ttctggtttt gaagatgtca gcatggatga gattaagagc
    33301 ttccgtcaat ggggttcaaa aacaccaggt cacccagaat ttggtcatac ggcagggatt
    33361 gatgctacga caggtcctct agggcaaggg atttcaactg ctactggttt tgctcaagca
    33421 gaacgtttct tggcagccaa atataaccgt gaaggctaca atatctttga ccactatact
    33481 tacgttatct gtggagacgg agacttgatg gaaggtgtct caagcgaggc agcttcatat
    33541 gcaggcttgc aaaaacttga taagttggtt gttctttatg attcaaatga catcaacttg
    33601 gatggtgaga caaaggattc ctttacagaa agtgttcgtg accgttacaa tgcctacggt
    33661 tggcatactg ccttggttga aaatggaaca gacttggaag ccatccatgc tgctatcgaa
    33721 acagcaaaag cttcaggcaa gccatctttg attgaagtga agacggttat tggatatggt
    33781 tctccaaaca aacaaggaac taatgctgta cacggcgccc ctcttggagc agatgaaact
    33841 gcatcaactc gccaagccct cggttgggac tacgaaccat ttgaaattcc agaacaagta
    33901 tatgctgatt tcaaagaaca tgttgcagac cgtggcgcat cagcttatca agcttggact
    33961 aaattagttg cagattataa agaagctcat ccagaactgg ctgcagaagt agaagccatc
    34021 atcgacggac gtgatccagt cgaagtgact ccagcagact tcccagcttt agaaaatggt
    34081 ttttctcaag caactcgtaa ttcaagtcaa gatgccttga atgttgtggc agctaaatta
    34141 ccaaccttcc taggtggatc agctgacctt gctcactcaa acatgactta tatcaagaca
    34201 gatggacttc aagacgatgc caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa
    34261 tttgcaatgg gaacaatctt gaacgggatg gcccttcatg gtggacttcg tgtatacggt
    34321 ggaactttct tcgtcttctc tgactatgtg aaggcagctg tccgcttgtc agccttacaa
    34381 ggacttcctg tgacttatgt ctttacccat gattcaatcg cagttgggga agatggtccg
    34441 actcacgaac cagttgagca tttagcaggt cttcgtgcta tgccaaatct aaatgttttc
    34501 cgtccagcag atgcgcgtga aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa
    34561 acaccaactg cccttgtctt gacacgtcaa aacttgactg ttgaagatgg aacagacttc
    34621 gataaggttg ctaaaggtgc ttatgttgta tatgaaaatg cagccgactt tgataccatc
    34681 ttgattgcga caggttcaga ggttaatctt gctgtctcag ctgctaaaga attggctagt
    34741 caaggcgaaa aaatccgcgt agtcagcatg ccatctacag atgtctttga taaacaagat
    34801 gcagcttaca aggaagaaat tcttccaaat gcagtccgcc gtcgtgttgc aattgaaatg
    34861 ggtgcaagtc aaaactggta caaatatgtt ggtctcgatg gtgccgttct aggtattgat
    34921 accttcggag cctctgcccc agcaccaaaa gtattggcag aatatggctt tactgtagaa
    34981 aatcttgtaa aagttgttcg aaacttgaaa taatcctaaa aatcagagcg taagctctgg
    35041 tttttcttac cagaaaagta aggtacaatc ttgtaaaagt agctgaaatt tgatatagta
    35101 gtcctatgta aaagacaaag gagaagacaa tgaatccaaa ttatacattt ttaatcatgc
    35161 ttgtaggtat gatggccttg atgttcttta tgcaacgttc tcaaaagaaa caagctcaaa
    35221 agcgtatgga aagcttaaat aaactacaaa aaggctatga agtgattaca atcggtggac
    35281 tttatggaac agtcgatgaa gtagatacgg agaagggaac aatagttctt gacgtagatg
    35341 gagtttactt gacttttgaa ctagctgcta tcaagacagt attaccgctg aaagaaacag
    35401 cttcactaga aggcgcaatt gaaaaataag acgggatcac gaactcccgt ttttctataa
    35461 aagaaaggaa atgggatgaa aaaattagtc tttgtctgtc tgggaaatat ttgccgtagc
    35521 cctatggccg agtttgttat gaaatcaatg acagataact acgaaatcca aagtcgagca
    35581 acttcctctt gggaacatgg caatccgatt cataagggga ctcagggaat ttttcaagag
    35641 tatgagattc cttatgacaa gaacaagaca tcgcttcaga ttagtaagga agattttgaa
    35701 gcctttgatt atattatcgg aatggacgct tcaaatattt ctgacttacg tcagatgtgt
    35761 ccagtagact gtcaagataa gatttactca ttttcatctg aaagtgttcc agacccttgg
    35821 tatacaggag attttgaaga aacctatcga cgtgttcaag agggttgtca agcttggtta
    35881 gaacgtttag aaaaggagag ttaaggtgga aaatcctacg aatttttacg aaaagtatcg
    35941 tgtctatctg actcgtccac gtttagagct tttggcagta gttaccattg ttttctgtgc
    36001 tgtactcgtc ttttttctaa atattccagg aaaaggtgtc ttaaaactcg ataatggaac
    36061 gattgtttat gatggcagtc ttgtccgtgg taaaatgaat ggtcaaggta ccattacctt
    36121 ccaaaatgga gaccaatata caggtggctt caacaatgga gccttcaacg gaaaaggtac
    36181 ctttcaatct aaagaaggct ggacctacga aggtgatttt gtaaatggtc aggctgaagg
    36241 aaaagggaaa ctaacaacag aacaagaagt cgtttatgaa ggaactttta aacaaggcgt
    36301 ttttcaacaa aaataaagcc tccttatcca aaggaggtat tattagaacc acaaggtaag
    36361 cgcttacctg taaatccctt tctttccaaa tccctcttcc aagcaagttt gtgaaataaa
    36421 aaatatttga aataaatttc acaaacttta aagataaaac ctgataagaa aagaaaatga
    36481 gaaaagtttc acaagagttt aaaaattctt tgtgaaatgt ttatgaaact gtttacaaag
    36541 ttgaaataat gcgttataat aaacttgtga aaaaattaac aaaggatatc gttccttgaa
    36601 agctatggag gaaaatatgg ctgataaaaa aactgtgaca ccagaggaaa agaaactcgt
    36661 tgctgaaaaa cacgtagatg agttggttca aaaagctcta gttgcccttg aagaaatgcg
    36721 taaattggat caagagcaag ttgactacat cgttgccaaa gcatcagtag cagctttgga
    36781 tgcccacgga gaattggctt tacatgcctt tgaagaaaca ggacgtggtg tatttgaaga
    36841 taaagcaact aagaacttgt ttgcctgtga acacgtagta aacaacatgc gccacactaa
    36901 gacagttggc gttatcgaag aagacgatgt aacaggattg actcttattg ctgaaccagt
    36961 tggtgttgtt tgtggtatta ctccaacaac aaacccaaca tcaacagcga tcttcaaatc
    37021 attgatttca ttgaagacac gtaatccaat cgtctttgcc ttccatccat cagctcaaga
    37081 atcatcagct catgcagctc gtatcgtccg cgatgcagct atcgcagctg gtgctcctga
    37141 aaactgtgta caatggatta ctcaaccatc tatggaagca acaagtgccc ttatgaacca
    37201 cgaaggtgtt gcgacaatcc ttgcaacagg tggtaatgcc atggttaagg cggcttattc
    37261 atgtggtaaa ccagctcttg gggtaggtgc cggaaacgtt ccagcttatg ttgaaaaatc
    37321 agcaaacatt cgtcaagcag cacacgatat cgtcatgtct aaatcatttg ataacggtat
    37381 ggtctgtgca tctgaacaag cagttatcat tgataaagaa atttacgatg aatttgtagc
    37441 agagttcaaa tcttaccaca cttactttgt aaacaaaaaa gaaaaagctc ttcttgaaga
    37501 gttctgcttc ggtgtcaaag caaacagcaa aaactgtgct ggtgcaaaat tgaacgctga
    37561 catcgttggt aaaccagcaa cttggattgc agaacaagca ggatttacag ttccagaagg
    37621 aacaaacatt cttgctgcag aatgtaaaga agttggcgaa aatgagccat tgactcgtga
    37681 aaaattgtca ccagttattg cagttttgaa atctgaaagc cgtgaagatg gtattactaa
    37741 ggctcgtcaa atggttgaat ttaacggtct tggacactca gcagctatcc acacagctga
    37801 cgaagaattg actaaagaat ttggtaaagc tgttaaagct attcgtgtta tctgtaactc
    37861 accttctact tttggtggta tcggggacgt ttacaatgcc ttcttgccat cattgacact
    37921 tggatgtggt tcttacggac gcaactcagt tggggataac gttagtgcca ttaacctctt
    37981 gaatatcaaa aaagtcggaa gacggagaaa taacatgcaa tggatgaaac ttccttcaaa
    38041 aacatacttt gaacgtgatt caattcaata ccttcaaaaa tgtcgtgacg ttgaacgtgt
    38101 catgatcgtt actgaccatg ccatggtaga gcttggtttc cttgatcgta tcatcgaaca
    38161 actggacctt cgtcgcaata aggttgttta ccaaatcttt gcggatgtag aaccggatcc
    38221 agatatcaca actgtaaacc gtggtactga gattatgcgt gccttcaaac cagataccat
    38281 catcgcactc ggtggtgggt ctccaatgga tgctgccaaa gtaatgtggc tcttctacga
    38341 gcaaccagaa gtggacttcc gtgaccttgt ccaaaaattc atggatatcc gtaaacgtgc
    38401 cttcaagttc ccattgcttg gtaagaagac taaattcatc gcgattccaa ctacatctgg
    38461 tacaggatct gaagtaacac catttgccgt tatctctgat aaagcaaaca accgtaaata
    38521 cccaatcgct gactactcat tgacaccaac tgtggcaatc gtagatcctg ctttggtatt
    38581 gacagttcca ggatttgttg ctgctgatac tggtatggac gtattgactc acgcgacaga
    38641 agcatacgta tcacaaatgg ctagtgacta cactgatggt ttagcacttc aagccattaa
    38701 attggtcttt gaaaatctcg aaagctcagt taagaatgca gacttccact cacgtgagaa
    38761 aatgcataac gcttcaacaa tcgctggtat ggcctttgcc aatgccttcc taggtatttc
    38821 tcactcaatg gcccataaga ttggtgcgca attccacaca atccacggtc gtacaaatgc
    38881 tatcttgctt ccatacgtta tccgttacaa cggtacacgt ccagctaaga cagcaacatg
    38941 gcctaagtac aactactacc gtgcagatga aaaataccaa gatatcgcac gcatgcttgg
    39001 acttccagct tctactccag aagaaggggt tgaatcttac gcaaaagctg tctacgaact
    39061 cggtgaacgt attgggatcc aaatgaattt tagagaccaa ggaattgacg aaaaagaatg
    39121 gaaagaacat tctcgtgaat tagccttcct ggcttatgaa gaccaatgtt caccagctaa
    39181 cccacgtctt ccaatggtag accatatgca agaaatcatc gaagatgcat actatggcta
    39241 caaagaaaga ccaggacgcc gtaaataatt gtttatcagt ctagaagcaa gacaaaaact
    39301 caatttgagg gaaagatcca gtaatttttc tatgataaaa ggcatcctat caaggttttt
    39361 gaacacctga taggatgcct ttttatgata ttgaggcctt tttgcccttt ttgaaaaact
    39421 agaatagaaa caaaatatat aatagattga aactagagta gtacacatct gcttctaaaa
    39481 cattgttaga attcgat
//
LOCUS       DBERCA010000013        39329 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.013, whole genome shotgun sequence.
ACCESSION   DBERCA010000013 DBERCA010000000
VERSION     DBERCA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.013"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..177
                     /locus_tag="ACL719_000791"
     CDS             <1..177
                     /locus_tag="ACL719_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0698796.1"
                     /translation="DKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKIS
                     EPEKLYAYIAEATA"
     gene            215..520
                     /locus_tag="ACL719_000792"
     CDS             215..520
                     /locus_tag="ACL719_000792"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0698797.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            568..2043
                     /locus_tag="ACL719_000793"
     CDS             568..2043
                     /locus_tag="ACL719_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGS0698798.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYATKAK"
     gene            2069..2260
                     /locus_tag="ACL719_000794"
     CDS             2069..2260
                     /locus_tag="ACL719_000794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698799.1"
                     /translation="MKIKEQTRKLAAGCSKHSFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHIYGKATLTKFELDFRRV"
     gene            2348..9049
                     /gene="bgaA"
                     /locus_tag="ACL719_000795"
     CDS             2348..9049
                     /gene="bgaA"
                     /locus_tag="ACL719_000795"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGS0698800.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWYF
                     KLNANSKEALKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIINTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRAIIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGATGQITLTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSASSQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9359..9736)
                     /locus_tag="ACL719_000796"
     CDS             complement(9359..9736)
                     /locus_tag="ACL719_000796"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0698801.1"
                     /translation="MFDKKQKRGYKESAKNLANWHYNDKEDSYTHPDGWYYRFHHTKH
                     QKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQGRQIFAQRKI
                     DVEPVFGQIKASLGYKRCNLRGK"
     gene            complement(9901..10146)
                     /locus_tag="ACL719_000797"
     CDS             complement(9901..10146)
                     /locus_tag="ACL719_000797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698802.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEDCHFHAF
                     YHAFDRLSYHLKMLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            complement(10358..10717)
                     /locus_tag="ACL719_000798"
     CDS             complement(10358..10717)
                     /locus_tag="ACL719_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGS0698803.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            10809..11765
                     /locus_tag="ACL719_000799"
                     /pseudo
     CDS             10809..11765
                     /locus_tag="ACL719_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11749..12306
                     /locus_tag="ACL719_000800"
     CDS             11749..12306
                     /locus_tag="ACL719_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0698804.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12299..12553
                     /locus_tag="ACL719_000801"
     CDS             12299..12553
                     /locus_tag="ACL719_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698805.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12565..14619
                     /locus_tag="ACL719_000802"
     CDS             12565..14619
                     /locus_tag="ACL719_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGS0698806.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLIWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14803..15663
                     /locus_tag="ACL719_000803"
     CDS             14803..15663
                     /locus_tag="ACL719_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGS0698807.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15874..16584
                     /gene="ccdA2"
                     /locus_tag="ACL719_000804"
     CDS             15874..16584
                     /gene="ccdA2"
                     /locus_tag="ACL719_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS0698808.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16604..17170
                     /locus_tag="ACL719_000805"
     CDS             16604..17170
                     /locus_tag="ACL719_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGS0698809.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17181..18293
                     /gene="msrB"
                     /locus_tag="ACL719_000806"
     CDS             17181..18293
                     /gene="msrB"
                     /locus_tag="ACL719_000806"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS0698810.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18561..19298
                     /locus_tag="ACL719_000807"
     CDS             18561..19298
                     /locus_tag="ACL719_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS0698811.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19295..20986
                     /locus_tag="ACL719_000808"
     CDS             19295..20986
                     /locus_tag="ACL719_000808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0698812.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSAGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21102..21665
                     /locus_tag="ACL719_000809"
     CDS             21102..21665
                     /locus_tag="ACL719_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGS0698813.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            21687..27350
                     /locus_tag="ACL719_000810"
     CDS             21687..27350
                     /locus_tag="ACL719_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS0698814.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDPTKYTDEET
                     VLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPIEGTTVETR
                     EEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAPTITETVIE
                     KPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSIKVALKDNT
                     GTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKEVQLDLKKV
                     EIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLAITNIEEVT
                     EEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDLVDAMQKNP
                     NGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFDRVEGGSIK
                     NINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDKLDDAGIMT
                     NVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIVGHLAHDFN
                     PRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKRGDIFFGSN
                     DMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMGITADTFEV
                     TEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQGNKLTTDS
                     PLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVKQVKEYSVA
                     ELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVNAIKNLYLE
                     ESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGLTYLNRYYG
                     VKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFREVVAKNTLS
                     GNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYRLYEGLNNG
                     QHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRETFKSEINKRAKEQVN
                     YLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPDYAPIREFF
                     GRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETTHVNDRMAY
                     LGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQWYNPDPTKL
                     QTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRPMDRNKLSG
                     PHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTAYVNVNMMA
                     GIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKENNGLLGDDF
                     IIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKTMFDEAVTK
                     DLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            27419..29140
                     /gene="pabB"
                     /locus_tag="ACL719_000811"
     CDS             27419..29140
                     /gene="pabB"
                     /locus_tag="ACL719_000811"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGS0698815.1"
                     /translation="MHRKTVIDFRTLGERYTFTKPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            29141..29758
                     /locus_tag="ACL719_000812"
     CDS             29141..29758
                     /locus_tag="ACL719_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0698816.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSRCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29808..30806
                     /locus_tag="ACL719_000813"
     CDS             29808..30806
                     /locus_tag="ACL719_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HGS0698817.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            30933..31892
                     /locus_tag="ACL719_000814"
     CDS             30933..31892
                     /locus_tag="ACL719_000814"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGS0698818.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31989..32828
                     /locus_tag="ACL719_000815"
     CDS             31989..32828
                     /locus_tag="ACL719_000815"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGS0698819.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32876..33046)
                     /locus_tag="ACL719_000816"
     CDS             complement(32876..33046)
                     /locus_tag="ACL719_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGS0698820.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33167..34051
                     /gene="miaA"
                     /locus_tag="ACL719_000817"
     CDS             33167..34051
                     /gene="miaA"
                     /locus_tag="ACL719_000817"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGS0698821.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            34044..35282
                     /gene="hflX"
                     /locus_tag="ACL719_000818"
     CDS             34044..35282
                     /gene="hflX"
                     /locus_tag="ACL719_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGS0698822.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            35275..35898
                     /locus_tag="ACL719_000819"
     CDS             35275..35898
                     /locus_tag="ACL719_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGS0698823.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLKEAAAFSGKRYFRSENRTAMLYID"
     gene            35913..36842
                     /gene="rnz"
                     /locus_tag="ACL719_000820"
     CDS             35913..36842
                     /gene="rnz"
                     /locus_tag="ACL719_000820"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGS0698824.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAKKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKDDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36863..37618
                     /locus_tag="ACL719_000821"
     CDS             36863..37618
                     /locus_tag="ACL719_000821"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0698825.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37672..38580)
                     /locus_tag="ACL719_000822"
     CDS             complement(37672..38580)
                     /locus_tag="ACL719_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS0698826.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38644..38913
                     /locus_tag="ACL719_000823"
     CDS             38644..38913
                     /locus_tag="ACL719_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGS0698827.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38913..39293
                     /locus_tag="ACL719_000824"
     CDS             38913..39293
                     /locus_tag="ACL719_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS0698828.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT        12070 a         7354 c         8757 g        11148 t
ORIGIN      
        1 gataaggaag tagaagtatc cctactcttc tttattatta acaattcaag ttcaagtcaa
       61 acaaatattc tggctcagtt gatggacttt ttcacaggaa atggacatct tgaagaccta
      121 tcaaaaattt ccgaaccaga aaaactttat gcttacattg ctgaagcaac cgcttaatct
      181 tgtctattaa aaaataaaat cggaggaaat ccaaatgatt aaaattcttg ctgcctgcgg
      241 tgcaggtgtt aactcaagtc accaaattaa aagtgctcta gaagaagaac tttcaaaccg
      301 tggttatgat gttcactgtg atgcagtcat ggtgaaagat gtaaacgaag accttatgaa
      361 aggttacgat atctttacac caatcgctgc aacagacctt ggttttgaac caggtatccc
      421 agttatcgaa gctgggccaa tcttattccg tatcccagca atgagcgctc cagtatttga
      481 caatattgaa gcagctatta aagaacacgg attaagctaa ttctaatttg ttaacattca
      541 tataacataa aaaagggagg aactgttatg gatgcaatct ttgacctaat cggaaaggtt
      601 ttcaatccca tcttagaaat gggtggacct gtcatcatgt tgatcatttt gacagtattg
      661 gctttacttt ttggagtgaa attctccaaa gcgcttgaag gtggtatcaa acttgccatc
      721 gctcttacag gtatcggtgc tatcatcggt atgctaaaca ctgctttctc agcatcactt
      781 gcaaaattcg ttgaaaacac tggtatccaa ttgagtatta ccgacgttgg ttgggcacca
      841 ctcgcaacaa tcacttgggg ttctgcttgg acactatact tcttgctcat catgttgatt
      901 gtcaacgtag tgatgcttgc tatgaagaaa accgatacac ttgatgtcga tatctttgat
      961 atctggcact tgtctatcac aggtctcttg attaaatggt atgctgataa caatggtgtg
     1021 agtcaagggg tttcactctt tattgctaca gcagctatcg tccttgtcgg tgtgttgaaa
     1081 attatcaact ctgacttgat gaaacctaca tttgatgacc ttcttaacgc cccaagttca
     1141 tcaccaatga catcaactca catgaactac atgatgaacc cagttatcat ggttttggat
     1201 aagatttttg aaaaattctt cccaggcctt gataaatatg actttgatgc tgctaaattg
     1261 aacaagaaaa tcggtttctg gggatctaaa ttcttcatcg gtttcatcct tggtatcgtt
     1321 atcggtatta tgggaactcc acatccaatt gcaggtgttg cagatgcaga taaatggcgt
     1381 cttgttatca aaggatggtt gtctcttggt ttgactgccg gtgtatcttt ggaactcttc
     1441 tcacttatcg gttcatggtt catcgcagcc gtagaaccac tatcacaagg tattacaaac
     1501 gttgctacta aacgtcttca aggacgtaaa ttcaatatcg gtcttgactg gccattcatc
     1561 gctggtcgtg ctgaaatctg ggcttgtgcc aacgtacttg caccaatcat gttgattgaa
     1621 gcagtgcttc tttcaaaagt tggaaatggt atcttgccac ttgcaggtat catcgctatg
     1681 ggtgttactc cagctctctt ggttgtaact cgtggtaaat tgctccgtat gattatcttc
     1741 ggaacactct tgttgccact cttccttctt tcaggtacac ttattgcacc atttgcaaca
     1801 gaacttgcta aaggtgtagg tgccttccca gaaggtgtga gccaaactca attgattact
     1861 cactctactc ttgaaggacc aatcgaaaaa cttcttggtt ggacaattgg taacactaca
     1921 actggtgata tcaaagcaat ccttggtgca gtagtcttcc ttgtattcta tatcggtatc
     1981 tttgcttggt acagaaaaca aatgatcaaa cgtaacgaag agtacgcaac aaaagcaaaa
     2041 taatgcgctc ctataaaata taaacttaat gaaaatcaaa gagcaaacta ggaagctagc
     2101 cgcaggttgc tcaaaacaca gttttgaggt tgtagataag actgacgaag tcagctcaaa
     2161 acatggtttt gaggttgtag atgaaactga cgaagtcagt aatcatatct acggcaaggc
     2221 gacactgacg aagtttgaat tggattttcg cagagtgtaa attgttgaaa ctaggttgtc
     2281 ataccatgta taccacttgg gctttggtag tagctaactg cactaaatat aatataagga
     2341 ggtttttatg gggaaaggcc attggaatcg gaaaagagtt tatagcattc gtaagtttgc
     2401 tgtgggagct tgctcagtaa tgattgggac ttgtgcagtt ctattaggag gaaatatagc
     2461 tggagaatct gtagtttatg cggatgaaac acttattact catactgctg agaaacctaa
     2521 agaggaaaaa atgatagtag aagaaaaggc tgataaagct ttggaaacta aaaatgtagt
     2581 tgaaaggaca gaacaaagtg aacctagttc aactgaggct attgcatctg agaagaaaga
     2641 agatgaagcc gtaactccaa aagaggaaaa agtgtctgct aaactggaag aaaaagctcc
     2701 aaggatagaa tcacaagctt caagtcaaga aaaaccgctc aaggaagatg ctaaagctgt
     2761 aacaaatgaa gaaatgaatc aaatgattga agacaggaaa gtggatttta atcaaaattg
     2821 gtactttaaa ctcaatgcaa attctaagga agcccttaaa cctgatgcag acgtatctac
     2881 gtggaaaaaa ttagatttac cgtatgactg gagtatcttt aacgatttcg atcatgaatc
     2941 tcctgcacaa aatgaaggtg gacagctcaa cggtggggaa gcttggtatc gcaagacttt
     3001 caaactagat gaaaaagacc tcaagaaaaa tgttcgcctt acttttgatg gcgtctacat
     3061 ggattctcaa gtttatgtca atggtcagtt agtggggcat tatccaaatg gttataacca
     3121 gttctcatat gatatcacca aataccttca aaaagatggt cgtgagaatg tgattgctgt
     3181 ccatgcagtc aacaaacagc caagtagccg ttggtattca ggaagtggta tctatcgtga
     3241 tgtgacttta caagtgacag ataaggtgca tgttgagaaa aatgggacaa ctattttaac
     3301 accaaaactt gaagaacaac aacatggcaa ggttgaaact catgtgacca gcaaaatcat
     3361 caatacggac gacaaagacc atgaacttgt agccgaatat caaatcgttg aacgaggtgg
     3421 tcatgctgta acaggcttag ttcgtacagc gagtcgtacc ttaaaagcac atgaatcaac
     3481 aagcctagat gcgattttag aagttgaaag accaaaactc tggaccgttt taaatgacaa
     3541 acctgccttg tacgaattga ttacgcgtgt ttaccgtgac ggtcaattgg ttgatgctaa
     3601 gaaggatttg tttggttacc gttactatca ctggactcca aatgaaggtt tctctttgaa
     3661 tggtgaacgt attaaattcc atggagtatc cttgcaccac gaccatgggg cgcttggagc
     3721 agaagaaaac tataaagcag aatatcgccg tctcaaacaa atgaaggaga tgggagttaa
     3781 ctccatccgt acaacccaca accctgctag tgagcaaacc ttgcaaatcg cagcagaact
     3841 aggtttactc gttcaggaag aggcttttga tacttggtat ggtggcaaga aaccttatga
     3901 ctatggacgt ttttttgaaa aagatgccac tcacccagaa gctcgaaaag gtgaaaaatg
     3961 gtctgatttt gacctacgta ccatggtcga aagaggcaaa aacaaccctg ctatcttcat
     4021 gtggtcaatt ggtaatgaaa taggtgaagc taatggtgat gcccactctt tagcaactgt
     4081 taaacgtttg gtcaaggtta tcaaggatgt tgataagact cgctatgtta ccatgggagc
     4141 agataaattc cgtttcggta atggtagcgg agggcatgag aaaattgctg atgaactcga
     4201 tgctgttgga tttaactatt ctgaagataa ttacaaagcc cttagagcta agcatccaaa
     4261 atggttgatt tatggatcag aaacatcttc agctacccgt acacgtggaa gttactatcg
     4321 ccctgaacgt gaattgaaac atagcaatgg acctgagcgt aattatgaac agtctgacta
     4381 tgggaatgat cgtgtgggtt gggggaaaac agcaaccgct tcatggactt ttgaccgtga
     4441 caacgctggc tatgctggac agtttatctg gacaggtacg gactatattg gtgaacctac
     4501 accatggcac aaccaaaatc aaactcctgt taagagctct tactttggta tcgtagatac
     4561 agccggcatt ccaaaacatg acttctatct ctaccaaagc caatgggttt ctgttaagaa
     4621 gaaaccgatg gtacaccttc ttcctcactg gaactgggaa aacaaagaat tagcatccaa
     4681 agtagctgac tcagaaggta agattccagt tcgtgcttat tcgaatgctt ctagtgtaga
     4741 attgttcttg aatggaaaat ctcttggtct taagactttc aataaaaaac aaaccagcga
     4801 tgggcggact taccaagaag gtgcaaatgc taatgaactt tatcttgaat ggaaagttgc
     4861 ctatcaacca ggtaccttgg aagcaattgc tcgtgatgaa tctggcaagg aaattgctcg
     4921 agataagatt acgactgctg gtaagccagc ggcagttcgt cttattaagg aagaccatgc
     4981 gattgcagca gatggaaaag acttgactta catctactat gaaattgttg acagccaggg
     5041 gaatgtggtt ccaactgcta ataatctggt tcgcttccaa ttgcatggcc aaggtcaact
     5101 ggtcggtgta gataacggag aacaagccag ccgtgaacgc tataaggcgc aagcagatgg
     5161 ttcttggatt cgtaaagcat ttaatggtaa aggtgttgcc attgtcaaat caactgaaca
     5221 agcagggaaa ttcaccctta ctgcccactc tgatctcttg aaatcgaacc aagtcactgt
     5281 ctttactggt aagaaagaag gacaagaaaa gactgttttg gggacagagg tgccaaaagt
     5341 acagaccatt attggagagg cacctgaaat gcctaccact gttccgtttg tatacagtga
     5401 tggtagccgt gcagaacgtc ctgtaacctg gtcttcagta gatgtgagca agcctggtat
     5461 tgtaacggtg aaaggtatgg ctgacggacg agaagtagaa gctcgtgtag aagtgattgc
     5521 tcttaaatca gagctaccag ttgtgaaacg tattgctcca aatactgact tgaattctgt
     5581 agacaaatct gtttcctatg ttttgactga tggaagtgta caagagtacg aagtagatag
     5641 ctgggagatt gccgaagaag ataaagctaa gttagcaatt ccaggttctc gtattcaagc
     5701 gaccggttat ttagaaggtc aaccaattca tgcaaccctt gtggtagaag aaggcaatcc
     5761 tgcagcacct gtagtgccaa ctgttactgt tggaggtgaa gcagtaacag gtcttactag
     5821 tcgacaacca atgcaatatc gtactctatc ttatggtgcc caattgccag aagtcacagc
     5881 aagtgctgaa aatgctgatg tgacagttct tcaagcaagc gcagcaaacg gcatgcgtgc
     5941 gatcatcttt attcagccta aagatggtgg ccctcttcaa acctatgcaa ttcaattcct
     6001 tgaagaagcg ccaaaaattg ctcacttgag cttgcaagtg gaaaaagctg acagtctcaa
     6061 agaagaccaa actgtcaaat tgtcggttcg agctcactat caagatggaa cgcaagctgt
     6121 attaccagct gataaagtaa ccttctctac aagtggtgaa ggggaagtcg caattcgtaa
     6181 aggaatgctt gagttgcata agccaggagc agtcactctc aaagcggaat atgagggagc
     6241 tacaggtcaa atcactctca ctatccaagc caatactgag aagaagattg cgcaatctat
     6301 ccgtcctgta aatgtagtca cagatttgca tcaaaaacct actcttccaa caacagtaac
     6361 ggttgagtat gacaaaggtt tccctaaagc tcacaaagtc acttggcaag ctattccgaa
     6421 agaaaaacta gactcttatc aaacatttga agtactaggt aaagttgaag gaattgacct
     6481 tgaagcgcgt gcaaaagtct ctgtagaagg tatcgtttca gttgaagaag tcagtgtgac
     6541 aactccaatc gcagaagcac cacaattacc agaaagcgtt cggacatatg attcaaatgg
     6601 tcacgtttca tcagctaagg ttgcatggga tgcgattcgt atagagcaat acgctaagga
     6661 aggtgtcttt acagttaatg gtcgcttaga aggtactcaa ttaacaacta aacttcatgt
     6721 tcgcgtatct gctcaaactg agcaaggtgc aaacatttct gaccaatgga ccggttcaga
     6781 attgccactt gcctttgctt cagattcaaa tccaagcgac ccagtttcaa atgttaatga
     6841 caagctcatt tcctacaata accaaccagc caatcgttgg acaaactgga atcgtagtaa
     6901 tccagaagct tcagtcggtg ttctgtttgg agattcaggt atcttgagca aacgctccgt
     6961 tgataatcta agtgtcggat tccacgaaga ccatggagtt ggtgcaccga agtcttatgt
     7021 gattgagtat tatgttggta agactgtccc aacagctcct aaaaacccta gttttgttgg
     7081 taatgaggac catgtcttta atgattctgc caactggaaa ccagttacta atctaaaagc
     7141 ccctgctcaa ctcaaggctg gagaaatgaa ccactttagc tttgataaag ttgaaaccta
     7201 tgctattcgt attcgcatgg ttaaagcaga taacaagcgt ggaacgtcta tcacagaggt
     7261 acaaatcttt gcgaaacaag ttgcggcagc caaacaagga caaacaagaa tccaagttga
     7321 cggcaaagac ttagcaaact tcaaccctga tttgacagac tactaccttg agtctgtaga
     7381 tggaaaagtt ccggcagtca cagcaaatgt tagcaacaat ggtctcgcta ccgtcgttcc
     7441 aagcgttcgt gaaggtgagc cagttcgtgt catcgcgaaa gctgaaaatg gcgacatctt
     7501 aggagaatac cgtctgcact tcactaagga taagaactta ctttctcata aaccagttgc
     7561 tgcggttaaa caagctcgct tgctacaagt aggtcaagca cttgaattgc cgactaaggt
     7621 tccagtttac ttcacaggta aagacggcta cgaaacaaaa gatttgtcag ttgaatggga
     7681 agaagttcca gcagaaaatc tgacaaaagc aggtcaattt accgttcgag gccatgtcct
     7741 tggtagtgat cttgttgctg aggtcactgt acgagtgaca gacaaactag gcgaagctct
     7801 ttcagataac cctaactatg atgaaaacag taaccaggcc tttgcttcag caaccaatga
     7861 tattgacaaa aactctcatg accgcgttga ctatctcaat gacggagatc attcagaaaa
     7921 tcgtcgttgg acaaactggt ctccaacacc atcttctaat ccagaagtat cagcgggtgt
     7981 gatcttccgt gaaaatggta agattgtaga acggactgtt gcgcaagcca aacttcactt
     8041 ctttgcagat agtggtacgg atgcaccaac taaactcgtt ttagaacgct atgtcggtcc
     8101 agagtttgaa gtgccaacct actattcaaa ctaccaagcc tacgacgcag accatccatt
     8161 caacaatcca gaaaattggg aagctgtgcc ttatcgtgcg gataaagaca tcgcagctgg
     8221 tgatgaaatc aacgtaacat ttaaagctgt caaagccaaa gctatgagat ggcgtatgga
     8281 gcgtaaagca gataagagcg gtgttgcgat gattgagatg accttccttg caccaagtga
     8341 attgcctcaa gaaagcacgc aatcgaagat tcttgtagat ggaaaagaac ttgctgattt
     8401 cgctgaaaat cgtcaagact atcaaattac ctataaaggt caacggccaa aagtctcagt
     8461 tgaagaaaac aatcaagtag cttcaactgt ggtagatagt ggagaagata gccttccagt
     8521 acttgttcgc ctcgtttcag aaagtggaaa acaagtcaag gaataccgta tccacttgac
     8581 taaggaaaaa ccagtttctg agaagacagt tgctgctgta caagaagatc ttccaaaact
     8641 cgaatttgtt gaaaaagatt tggcctacaa gacagttgag aaaaaagatt caacactgta
     8701 tctaggtgaa actcgtgtag aacaagaagg aaaaactggt aaagaacgta tctttacagc
     8761 gattaatcct gatggaagta aggaagaaaa actccgtgaa gtggtagaag ctccgacaga
     8821 ccgcatcgtc ttggttggaa ccaaaccagt agctcaagaa gctaaaaaac cacaagtgtc
     8881 agaaaaagca gatacaaaac caattgattc aagtgaagct agtcaaacta ataaagccca
     8941 gttaccaaat acaggtagtg cgtcaagcca agcagcagta gcagcaggtt tagctcttct
     9001 aggtttgagt gcaggattag tagttactaa aggtaaaaaa gaagactaga aattttgata
     9061 ccttctttat cattaaagac caaagaaaaa ctcttgtcct aaatagaaga taaagagtct
     9121 ggaacaaaat agttctcaac cacaaaaaag ctagatattt ccaattgtgg aactctagct
     9181 ttttaatttt gagtcattct cttattgtat agtggattga aactagaata gtacacttct
     9241 acttctaaaa cattgttaga aatcgatttg actgtcctta tcgatttgtc ctgttcttat
     9301 ttcattttac tatattttag gaggttattg gccataagta ccaatcccat gtcaattctc
     9361 acttgcccct cagattacat ctcttgtaac ccaaagaagc ctttatctgc ccaaagacag
     9421 gttccacatc aatcttgcgt tgagcgaaaa tctgtctacc ttggggagat aaaagcgcct
     9481 gacattcttt agctttcaag ttttgatagc gttcgttcat atatagtccc ttttgagggg
     9541 ctgattcagg ttcgtcggcg tagtaaacct tgatttcctg ttgaaagtct gtctgtgttt
     9601 tctgatgttt ggtatggtga aaacgataat accagccatc aggatgtgtg tagctatcct
     9661 ccttgtcatt atagtgccaa ttcgctaagt ttttagctga ctctttatac cctctctttt
     9721 gtttcttatc aaacatggca tatttaatca ggaggggagt gatttcttct tgaaaataga
     9781 cttgtatctg ttcttgaagt ttggcggaga atttctctgt cgttttcttc cacacaaaac
     9841 tatacttgtt ggcattggct tcaatcttag ttccgtcaat aaacaaacaa tctaaggtca
     9901 ctaactcttc catttttcaa tttttcgacc agagaaaatc ccttgtgaat aggcaaatag
     9961 aagagtagat acaagcattt taaggtgata agacaggcga tcaaaggcat gatagaaggc
    10021 gtggaaatga caatcctcca aggtattcac cactttttca atagtaaaaa cgagatggtc
    10081 ttgtggcaag aaggaactga tttctagtgg taaagttgtt tgatttgtgt tatagtgaat
    10141 atgcatggga tagcctttct aaatggttta ttgggcaatt ctattttacc aagactatcg
    10201 taaccatgca aaaaaagcaa cggcaaatcc gttgcttttt ttggagataa gagactattg
    10261 tcccagactc ttttctttgt ataaaattgt atagtgttat tgtctatact attttgtaga
    10321 gaaagaaaaa atccctagaa aatgcttagg gattcgacta tttcattaag aacaattcaa
    10381 caatcatttc tacatacatg ataagggtta ataagaatcc tgcacgccag aagaatttga
    10441 tgaatttagg atagtaaaag ctgcgttttt tcaaaaggaa gaaaaagacg agaataatgg
    10501 ctaggatcga gagggctagc cccagtctag ggaggaaatt atgggtaaag gttttagctg
    10561 taatcaggta atattcaaat accaagaccg gaaaagccaa atccgcaaag tttcgtccta
    10621 gtttttttaa tctaaaaagt ttggttagga taatgcagac gactaaggtc agtatcaata
    10681 ataaaataga tgctaatttc attaaaatca tacccatatt gtatcataaa aaaaggctaa
    10741 tggaaacgag aaacagagga atttatagga aagtcaggct tttgagattg tgggtgattt
    10801 ttgttataat gaaagtcatg aaatcttata ataccttgaa tgattattat cgaaaattct
    10861 ttggagaaaa gacttttaaa gtccctattg atgcgggatt tgactgtccc aatcgtgatg
    10921 gaactgtggc tcatggaggc tgtacttttt gtacggtttc aggttctgga gatgctattg
    10981 tagccccaga tgcgcctatc cgtgagcaat tttataagga aattgacttt atgcaccgta
    11041 agtggccgga tgttcagaag tatctggttt attttcaaaa ttttaccaac acccatgaaa
    11101 aggtggaagt tattcgagag cgttatgaac aggctatcaa tgagccaggt gtggtaggta
    11161 tcaatattgg aacgcgccca gactgtttac cagacgaaac catcgaatat ttggctgagt
    11221 tatcggagtg catgcatgtg acggttgaat tgggcttgca gaccacttat gaagcaacct
    11281 ctgacctgat taaccgtgtg cattcttatg aattgtaggt ggaaacggtc aagcgtttga
    11341 gaaagtatcc caagattgag attgtttccc atttgatcaa tggcttgcct ggtgaaaccc
    11401 atgagatgat ggttgaaaat gtccgccgtt gtgtcacgga taacgatatt caagggatta
    11461 aactgcattt gctccatctt atgacaaata ctcgtatgca acgagattac catgagggac
    11521 gtttacaatt gatgagtcag gacgaatatg tcagggtcat ctgtgaccaa ctggaaatta
    11581 ttcccaaaca tatcgtcatc catcgaatta caggagatgc gcctagggat atgctgattg
    11641 gtcctatgtg gagcctcaaa aaatgggaag tcctcaacag cattgagatg gagatgcgac
    11701 gtcgtggaag tgttcaagga tgcaaggctg taaaacagga gtttgaaaat gaaaagacca
    11761 cttgagatgg cacatgattt tttggctgag gtcgtgacaa aagaggatgt cgtagtggat
    11821 gcgactatgg gaaatggtca tgacacgctt tttttagcca agctagccaa gcaagtctat
    11881 gcctttgata ttcagaagca agccttggaa aagacccaag agcgtttgca tcaggctggc
    11941 ttgacaaatg cccagttaat cttgcaaggc catgagacac tggaccagtt tgtgacaaaa
    12001 gctaaggcag ggatttttaa tctgggctat ttgccgtcag ctgataagtc tgtcatcacc
    12061 cgaccgcaga caacgattga ggcattagaa aagctatgtg gcttacttgt caaaggtgga
    12121 cgaattgcta ttatgattta ctatggtcat gaaggaggcg acctcgagag agatgctgtc
    12181 ttggattttg tgatccagtt gaaccaacaa gagtacacag ctgccattta ccgaacttta
    12241 aaccaagtca acaacccgcc gtttttagtg atgattgaaa aattagagag atacagacat
    12301 ggataaacaa tacctacacg aaaagctgga tgccatgcgc cagaattttg ttgaatcaac
    12361 tcaccacgaa cgagcggtgg gcgtgctaga ccaagcgcat atgagcaaaa aaatgcttaa
    12421 aatcaagaaa aaattagttg ctcttgaaat ggaacggtgc cagagaaaaa ttgagcacaa
    12481 agactgttcc aagattgacc aaaaaatcaa agagcagaag gagatatttg aatcctgttg
    12541 taaaaaagat taaggaggac gtgcgtggaa ttactgattt acctcatcct atttttactg
    12601 gttttgattg tctcaagtac aaccaataag ctcctaccct ttttgcctct ccctttggtg
    12661 cagattcttt tgggaattgt gattggtctc tttttaccca atactgactt tcatcttaat
    12721 acggagttgt ttttggccct ggttatcgga cccttgcttt tccgagaggc tgaagaagca
    12781 gatgttacgg ctattttaaa acactggcga atcattgttt atctcatatt tccagtgatt
    12841 tttatctcga ccctgagttt gggtggcttg gcccatctta tttggttcag ccttcccttg
    12901 gcagcttgct tggctgttgg ggcagccctt ggtcctacgg acttggtggc ctttgcctct
    12961 ctttcggagc gttttagctt tcctaagcgc gtgtccaata ttcttaaggg cgaaggactc
    13021 ttgaatgatg cttctggttt ggtggctttt caggtagctt tgacagcttg gacaactgga
    13081 gctttttctc tggggcaagc tagcagttcg ctcatctttt caatcctagg cggtttttta
    13141 attggctttt taacagccat gaccaatcgt ttcctccata gcttcttgct aagtgtgaga
    13201 gcaacggata ttgctagtga gctccttctg gaattgagtt tgcccttggt aaccttcttc
    13261 ctggcagaag aagtccatgt ttcagggatt attgccgtcg tagttgctgg aattttaaag
    13321 gcaagtcgct tcaagaaaat cacgctcctc gaagcccaag tggatacggt gaccgagacg
    13381 gtctggcata cagtgacctt tatgctcaac ggttctgtct ttgtgatttt agggatggag
    13441 ttggaaatga tagcagaacc tatcttgacc aatccaatct ataatcctct acttttattg
    13501 ctatctctca tcgcccttac ctttgtcctc tttgtcattc gttttattat gatctatggc
    13561 tattatgcct atagaacccg acgcctcaag aaaaagctaa ataagtatat gaaggacatg
    13621 tttctcttga ccttttcagg tgttaaggga acggtgtcga ttgctacgat tctcttgata
    13681 ccaagtaatc tagaacagga gtatcctctc ttgcttttcc ttgttgcagg tgtgacgctt
    13741 gtcagctttt taacaggtct cttggtcttg cctcatcttt ctgatgaaga ggaagaaagc
    13801 aaggattatc tcatgcatat cgccattttg aatgaagtaa cgctagagtt ggaaaaagag
    13861 ttggaagaca ctagaaataa acttcccctc tatgcggcta ttgacaatta tcatggacgt
    13921 attgaaaatc tcattttaag ccaagaaaac caggatgatc aagaagactg ggctgctttg
    13981 aaactcttga ttcttagtat tgaaagtgat ggtttggaac aggcctatga agaggggaac
    14041 attagcaatc gtgcttaccg agtttaccaa cgttatctga aaaatataga acaaggaatc
    14101 aatcgtaaac ttgcctcaag actgacctat tattttcttg tttccttgag gattttacgt
    14161 tttctccttc atgaagtttt tactctcgga aagaccttcc gtagctggaa gaacaaggag
    14221 caaagccgtc tccgtgctct tgattatgac caaattgcag agctctatct tgccaataca
    14281 gagatgatta ttgaaagttt ggaaaacctg aagggagtct acagacgctc tttgattagt
    14341 tttatgcagg agtctcgtct tcgagaaaca gctattatca gcagtggtgc ctttgtcgaa
    14401 cgggttatca atcgtgtcaa acccaacaat atcgatgaaa tgctgagagg ctattatctg
    14461 gagcgcaagt tgattttcga atacgaagaa aaacgattga ttacgactaa gtatgccaag
    14521 aaattacgac aaaatgtaaa taacttagag aactattcct tgaaggaagc tgccaatacc
    14581 ctaccgtatg atatggtgga attggtaaga agaaattagt taatactctt cgaaaatctc
    14641 ttcaaaccac gtcagcgtcg ccttaccgta tgtatgtgac tgacttcgtc agtcttatct
    14701 acaacctcaa aacagtgttt tgagcagctt gcggctagct ttctagtttg ctctttgatt
    14761 ttcattgagt ataagattat aagtgaagga gtatgatatg acatgaaaaa gtggtggaaa
    14821 gagctgatag acaaaccttt attaaaagct tttttgcatt attatcaagc atcagatagt
    14881 gagttgacca gtgtcgcagt agcctactat tggttgattt cgattttccc cctgcttttg
    14941 gtggtggtca atatcctccc ctattttcag attcctgtgg gagaattttt gggctttatg
    15001 aaagacgttc tgcccccaag cctctatgaa ggtgtggaaa aaatagccag agaagttttg
    15061 acgcaacctt caacaggttt attgagtttt tctgttttat cggcgttatg gagtttttca
    15121 aaatctatga atttcttgca aaaagctttt aacaaggctt atggcgtcga aaagagtcgt
    15181 ggacttattt cccatcagat gctgagtctc ctagtcagtt ttggcttgca gctcctcttt
    15241 gcgtttgcct tgtttttgat cttatttggt cagatgattt tggctttact tgctcattat
    15301 tggacaaaag atggcattat ctatcaggct ttacaagggt tggcaggtcc tctgatttac
    15361 gccctcctct ttgctatctt ggtcatgctt tattattttc ttcccaatct atctaacagg
    15421 aaaattagct ataccctacc aggcagtgcc tttgttctct tagtgatttt aggtttacta
    15481 actctgttct ctagttatct caattactat gtccatcatt tggtggatgt ccgaatttta
    15541 ggttccgttc ttcttgttgt tatgatgttt tggtttatct tgattgctaa aattgttatc
    15601 ctaggcgcgg ttatcaatgc cagtatgcag agtttgaaag atccggtctt taaaaaagac
    15661 taactattta tctattataa aagcgcatag tatcaagact ttatagaacc ttgatactat
    15721 gcgcttttgt gctttgacag ttttttgtat agtttttgtc caagatttct aaatcatctg
    15781 attaaattcc tattttcccc tatcattgtc cctgtttttt cgaaaatgga aggagtataa
    15841 taaatctatc aataaaatga taggaggaac tttatgggtc atattttctt ttttctaagt
    15901 gtctttttgg cagggattct atccttcttt tctccttgta tcttaccttt gttaccggtc
    15961 tatacagggg tgttactaga tgataaggat ggtgctcagg cttctagcgg caaattttca
    16021 atctcagtta ctagtttatt acgaactctg gcctttatag caggaatttc ctttatattt
    16081 attttgttgg gctatggagc tggtttttta ggcgatttgc tttatgcttc ttggttccaa
    16141 tatcttactg gggcaattat tatccttctt ggtttgcacc aaatggagat tctacacttt
    16201 aaggggcttt ataaggaaaa gaggctacaa ctgcaaggac aggggcaaaa tggtaagggc
    16261 tatagtcagg catttttatt gggcttgacc tttagttttg cttggacgcc ttgcgtgggg
    16321 ccggttctgg ggtctgtttt ggccttggcg gcttcaggtg gttcaggagc ttggcaggga
    16381 gctggtctca tgttggtgta tacgctgggc ttggcgctac cattcttgct tctagctctg
    16441 acctctagtt atgttttgaa acatttccga aaacttcatc cctatctcgg aatcctcaaa
    16501 aaagtgggag gttttctcat tattgtgatg ggactcttgg ttctgtttgg aaatgcttca
    16561 attttaagtc aattatttga ataaaatgga aaggaatatc aatatgaaaa aatggcaaac
    16621 atgtgttctt ggagcgggtt cgctcctttg tttgacggct tgttcaggca agtccgtgac
    16681 tagtgaacac caaacgaaag atgaaatgaa gacggagcag acagctagta aaacaagcgc
    16741 agctaaaggg aaagaggtgg ctgattttga attgatggga gtagatggca agacctaccg
    16801 tttatctgat tacaagggca agaaagtcta tctcaaattc tgggcttctt ggtgttccat
    16861 ctgtctggct agtcttccag atacggatga gattgctaaa gaagctggtg atgactatgt
    16921 ggtcttgaca gtagtgtcac caggacataa gggggagcaa tctgaagcgg actttaagaa
    16981 ttggtataag ggattggatt ataaaaatct cccagtccta gttgacccat caggcaaact
    17041 tttggaaact tatggtgtcc gttcttaccc aacccaagcc tttatagaca aagaaggcaa
    17101 gctggtcaaa acacatccag gattcatgga aaaagatgca attttgcaaa ctttgaagga
    17161 attagcctag gaggtgtctt atgaatgata agttaaaaat cttcttgttg ctaggagtat
    17221 tttttctagc cataaccggt ttctatgttc tattgatacg aaatgcaggg cagacagatg
    17281 cctcgcaaat tgaaaaggcg gcagttagcc aaggaggaaa aacagtgaaa aaaacagaaa
    17341 ttagtaaaga cgcagacttg cacgaaattt atctagctgg aggttgtttc tggggagtgg
    17401 aggaatattt ctcacgcgtt cccggggtga cggatgccgt ttcaggctat gcaaatggta
    17461 gaggagaaac aaccaagtac gaattgatta accaaacagg tcatgcagaa accgtccatg
    17521 tcacctatga tgccaagcaa atttctctca aggaaatcct gcttcactat ttccgcatta
    17581 tcaatccaac cagcaaaaat aaacaaggaa atgatgtggg gacccagtac cgtactggtg
    17641 tttattacac agatgacaag gatttggaag tgattaacca agtctttgat gaggtggcta
    17701 agaaatacga tcaacctcta gcagttgaaa aggaaaactt gaagaatttt gtggtggctg
    17761 aggattacca tcaagactat ctcaagaaaa atccaaatgg ctactgccat atcaatgtta
    17821 atcaggcggc ctatcctgtc attgatgcca gcaaatatcc aaaaccaagt gatgaggaat
    17881 tgaaaaagac cctgtcacct gaggagtatg cagttaccca ggaaaatcaa acagaacgag
    17941 ctttctcaaa ccgttactgg gataaatttg aatccggtat ctatgtggat atagcaactg
    18001 gggaacctct cttttcatca aaagacaaat ttgagtctgg ttgtggctgg cctagtttta
    18061 cccaacccat cagtccagat gttgtcacct acaaggaaga taagtcctac aatatgacgc
    18121 gtatggaagt gcggagccga gtaggagatt ctcaccttgg gcatgtcttt acggatggtc
    18181 cacaggacaa gggcggctta cgttactgta tcaatagcct ctctatccgc tttattccca
    18241 aagaccaaat ggaagaaaaa ggctacgctt atttactaga ttatgttgat taatactctt
    18301 cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtagggtta ctgacttcgt
    18361 cagttttatc tacaacctca aagctatact ttgagctgac ttcgtcagtt ttatctacaa
    18421 cctcaaagcg gtactttgag aaacctgtgg ctatcttcct agtttacttt ttgattttta
    18481 ttgagtataa gaggtctttc ctaagcagtt agaggagaat tttgctatac tgatactagt
    18541 aaatggtaaa ggagcagagc atgacctaca caatcttaat cgtagaagat gaatatctgg
    18601 taagacaagg tttgactaaa ctggtcaatg tagcagccta cgatatggaa atcatcggtc
    18661 aggctgaaaa tggaaggcag gcttgggaat tgatccaaaa gcaggttcca gatatcattt
    18721 taaccgatat caacatgcct catctaaatg gcatccagtt ggccagtttg gtacgagaaa
    18781 cctatcctca ggttcatttg gtctttttaa caggttacga tgattttgat tatgccttgt
    18841 ctgctgtcaa actaggtgtg gacgactacc tgctcaaacc cttttctcgt caggatattg
    18901 aggaaatgtt ggggaaaatc aaacaaaaac tagacaagga agagaaagaa gagcagttac
    18961 aagatttatt gactaatagg tttgaaggaa acatggccca gaaaatccag tctcatctgg
    19021 ctgatagcca atttagttta aagtctttag ccagtgactt aggttttagt ccgacctatc
    19081 tgagttcttt gattaagaaa gagttgggct tgccttttca ggattatctg gtgagagaac
    19141 gtgttaaaca agccaagctc ttgcttttaa ctacagatct gaagatttat gagatcgcag
    19201 agaaggttgg ttttgaagat atgaactatt ttacccaacg ttttaagcag attgcaggtg
    19261 tgacacctcg tcagtttaag aagggagaag accgatgaag cgttcttctc ttttagttag
    19321 aatggttatt tccatctttc tggtctttct cattctccta gctctggttg gaactttcta
    19381 ctatcaatca agttcttcag ccattgaggc caccattgag ggcaacagcc aaacgaccat
    19441 cagccagact agccacttta ttcagtctta tatcaaaaaa ctagaaacca cctcggctgg
    19501 tttgacccag cagacggatg ttctggccta tgctgagaat cccagtcaag acaaggtcga
    19561 gggaatccga gatttgtttt tgaccatctt gaagtcagat aaggacttga aaactgttgt
    19621 gctggtgacc aaatctggtc aggtcatttc tacagatgac agtgtgcaga tgaaaacttc
    19681 ctctgatatg atggctgagg attggtacca aaaggctatt catcagggag ctatgcctgt
    19741 tttgactcca gctcgtaaat cagatagtca gtgggtcatt tctgtcactc aagaacttgt
    19801 tgatgcaaag ggagccaatc ttggtgtgct tcgtttggat atttcttatg aaactctgga
    19861 agcctatctc aatcaactcc agttggggca gcagggcttt gccttcatta tcaatgaaaa
    19921 ccatgaattt gtctaccatc ctcaacacac agtttatagt tcgtctagca aaatggaggc
    19981 tatgaaaccc tacatcgaga caggtcaggg ttatactcct ggtcacaaat cctacgtcag
    20041 tcaagagaag attgcaggaa ctgattggac ggtacttggc gtgtcatcat tggaaaagtt
    20101 agaccaggtt cggagtcagc tcttgtggac cttgcttggg gccagtgtca catctcttct
    20161 tgtctgtctc tgcttagtgt ggttcagtct taaacgctgg attgctcctt tgaaggattt
    20221 gagagaaacc atgttggaaa ttgcttctgg tgctcaaaat cttcgtgcca aggaagttgg
    20281 tgcctatgaa ctgagagaag taactcgcca atttaatgcc atgttggatc agattgatca
    20341 gttgatggta gctattcgta gccaggaaga aacgacccgt cagtaccaac ttcaagccct
    20401 ttcgagccag attaatccac atttcctcta taacactttg gacaccatca tctggatggc
    20461 tgaatttcat gatagtcagc gagtggtgca ggtgaccaag tccttggcaa cctatttccg
    20521 cttggcgctc aatcaaggca aggacttgat ttgtctctct gacgaaatca atcatgtccg
    20581 ccagtatctc tttatccaga aacaacgcta tggagataag ctggaatacg aaattaatga
    20641 aaatgttgcc tttgataatt tagtcttacc caagctggtc ctacaacccc ttgtagaaaa
    20701 tgctctttac catggcatta aggaaaagga aggtcagggc catattaaac tttctgtcca
    20761 gaaacaggat tcgggattgg tcatccgtat tgaggatgat ggcgttggct tccaagatgc
    20821 tggtgatagt agtcaaagtc aactcaaacg tgggggagtt ggtcttcaaa atgtcgatca
    20881 acggctcaaa cttcattttg gagccaatta ccagatgaag attgattcta gaccccaaaa
    20941 aggaacgaaa gttgaaatat atataaatag aatagaaact agctaactcc cagtcaattc
    21001 tgggagtttt atgcgtaatt gggcaaactt tctaggttgt ctatcttgct aaaaacaaga
    21061 aaaaacggta tgatagaaga gtgacaacag aggtaacaga gatgaacgag acacagattc
    21121 aaagagaaac acgtcaggta gtggaagatg ttttagaaaa gaccaatttg aaacaggggg
    21181 ccctttttgt tttaggtcta tcttctagtg aggtgctagg tggtcagatt gggaaagaat
    21241 ccagtcaaga gattggtgag ctgatagtgg agactatctt gggtattcta ggtagtagag
    21301 gtatccattt agcagttcaa ggttgtgagc atgtcaatcg ggctctcgtt gttgaacgtc
    21361 aggtggcaga gcagtttggt ctggaaattg tcagtgtcca tcctactctt catgcaggag
    21421 gttcggggca gttggcagcc tttaagttta tgcaggatcc agttgaggtt gaatttatca
    21481 aggctcatgc tggattggat atcggagaca ctgcaattgg catgcatgtc aagcatgttc
    21541 aggttccgat tcgccctatt ttgagagaga ttggtcatgc ccatgtaacg gcactgacta
    21601 gtcgtccaaa attaatcgga ggtgcgcgtg cgcactatcc gcaagatgct attaggaagt
    21661 tttgagaatg aataaaggag ataaaagtgt ttaaaaaaga ccgtttttca attcgtaaga
    21721 ttaagggagt tgtaggctct gtatttcttg gaagcctttt gatggctcct tctgtagtgg
    21781 acgcagccac ctatcactat gtaaataaag agattatttc acaagaagct aaagatttaa
    21841 ttcagacagg aaagcctgac aggaatgaag ttgtatatgg tttggtgtat caaaaagatc
    21901 agttgcctca aacagggaca gaagcatctg ttttgacagc ttttggtttg ctgactgttg
    21961 ggagcttgct tttaatctac aagagaaaga aaattgctag cgtctttcta gttggaacta
    22021 tgggattggt agttcttcct agtgcagggg ctgtagaccc agttgcgacc ctagcactgg
    22081 ctagtcgaga gggtgttgtt gaaatggagg gctatcgcta tgttggttat ctatcaggtg
    22141 acatcctcaa aacgcttggc ttggacactg ttttagaaga aacctcagca aaacctggag
    22201 aggtgactgt agtcgaagtt gagactcctc aatcaacaac aaatcaggag caagctagga
    22261 cagaaaacca agtagtagag acagaggaag ctccaaaaga agaagcacct aaaacagaag
    22321 aaagtccaaa ggaagaacca aaatcggagg taaaacctac tgacgacacc cttcctaaag
    22381 tagaagaggg gaaagaagat tcagcagaac cagctccagt tgaagaagta ggtggagaag
    22441 ttgagtcaaa accagaggaa aaagtagcag ttaagccaga aagtcaacca tcagacaaac
    22501 cagctgagga atcaaaagtt gaacaagcag gtgaaccagt cgcgccaaga aaagacgaac
    22561 aggcaccagt cgagccagaa aatcaaccag aagctcctga agaagagaag gctgtagagg
    22621 aaacaccgaa acaagaagag tcaactccag ataccaaggc tgaagaaact gtagaaccaa
    22681 aagaggagac taaaacagct aaggggacgc aagaagaaga ttacgtgggt gaggctcttg
    22741 ttccagaaca attacctgag tataaggtga ctgaaggaac actcgttgaa gaatcaacta
    22801 cagaacttga ctataaaact gaaacgactg aggacccaac caagtacacg gacgaagaaa
    22861 ctgtcctacg aaatggtgaa aaaggaagtc aagttacaaa aacaacctat aaaacaatag
    22921 gaggggttaa aactgaccaa gttctttcaa ccagtacgga agtcaccaag gagcctgtaa
    22981 atcaacaggt aatccgtggt acaaaaccaa ttgaagggac aacggttgag acaagagaag
    23041 aaatcattcc ttttgaaaca aaagaacaag aagatgacac tttaaaacgc ggaacaagac
    23101 aagtaactca agaaggtgta aacgataaga aacaaattac tgaaacctat aaaacaattc
    23161 gcggagaaaa aacaagtgac gctcctacta tcacagaaac agtgattgaa aaacctcaag
    23221 acaaaattat taagaatggc acaaaagagt tagagaaacc aaccttggaa tgggctaaaa
    23281 cagataaaga tgtcttgaag aagagtgcga cagctagtta tacattgact aaaccagctg
    23341 gagtagaaat taaatcgatt aaagtagctc taaaagataa tactggaaca gttgttaaag
    23401 aagtaacggt agaagaaaat aacttacaag ctactttaga caacttgaaa tattatcaag
    23461 gttatacact atccacaacc atggtctatg atcgaggtaa cggtgaagaa actgaaatat
    23521 tagaagataa agaagttcag ttagacctta aaaaagttga gattaaagat attaaagaaa
    23581 caagattgat gaaagtggat gagaatggaa atgaaacaga tagtagcctt ctagaaactg
    23641 ttccagaaaa tctaacgtca tattatctaa aaattaccac taatcataat aaagtaacaa
    23701 ggcttgccat tactaatata gaggaagtaa ctgaggagga gacatcctta tttaaagtta
    23761 ctgcttcagc tcctgattta atccaacgtg attcaaataa tagtttgagt caaacataca
    23821 cttattacat agagaaaccc aaagcatcgc aaaataacgt ttactataac ttcaaagatt
    23881 tagtagatgc tatgcagaaa aatcctaacg gtgagtttaa attaggatcg gatttaaatg
    23941 caacaaatgt acctacacca agtaaatcat atgtaacagg taaatttaca ggtcacttaa
    24001 caagtgttga tggtaagcat ttctccattc ataacactgc tcgtccatta tttgatagag
    24061 ttgaaggggg ttctatcaaa aatattaatt taattaacgt taatattaat atgccttggg
    24121 caaatcaatt agcttcatta gctcaaacag tgaaaaatgc aactgttgaa aatattaaag
    24181 taacgggtac tatcgttggt aataacaatg ttgcgggtat tttggataaa ttagatgatg
    24241 caggtattat gactaatgtt gcttttatcg gaaaagttca atctgttggt gataaagggt
    24301 ggaacttggc tgggattgca tctgagattt atcgaggcaa tttaaataaa gcttatgcag
    24361 atgttgaact taaaggttta aatactcgtg caggtgctat agtaggacat ttagctcatg
    24421 attttaatcc aagagatgtt ggtgcttggt ctaatgtaag aaattctgta gccaaaggta
    24481 ctattgatgt tgctagaggt gatgtaggcg gtttaattgg taaagactgg ccttggggaa
    24541 ccattgataa caatatcagc ttagtgaatg ttaagcgtgg tgatattttc tttggttcta
    24601 atgatatgga tgatgaattt gtaagcaatt ggattaaacc taataatgca acagttatcg
    24661 gtgtgagttc tggagataaa acccataaac aatcgtctaa aattaaagaa atttcacagg
    24721 tagaagctga taagaaaatt aaggatatgg gtatcactgc tgatacattt gaagtaactg
    24781 agcatttggt tgataagttg aatcgccgta tattacgtga tgaagaatac aaaacaattc
    24841 aagattataa tgcaaatcgt gagcttgttt atcgtaatgt tgaaaaactc caaccgttct
    24901 acaacaaaga atggattgtg aatcaaggga acaaacttac aactgatagt ccattaatga
    24961 ataaagaagt attgtctgtg actgcaatga aaggtaatga ctttattaca gaccttacag
    25021 atgctgacca tattatggtt cattacgcag ataagacaaa agatatcttt acgatttctc
    25081 ctaaagactc tcaagttaag caagtaaaag aatacagtgt cgcagagctt ggtgaagtcg
    25141 tctatacacc aaatatggtt gttaaagatc gtgcagattt aattagtgct attgaaggta
    25201 ttttaagtcc aattgaatta caatctgatc caatttatca acgattagga agaactgggg
    25261 ataacagagt taatgcaatt aagaatcttt acttagaaga aagctttaaa gaagtaaaag
    25321 atcatcttaa tacatttgtg acaaaattag ttgaaaatga agatcatcaa ctgaatacag
    25381 atgaagctgc aaaacgtgcc ttgattaaga aaattgaaga taacaaagct gctgtcctgt
    25441 taggattaac atacctaaat cgttattacg gtgtgaaatt tgataacttc aatattaaag
    25501 aattaatgct attcaaacca gatttttatg gtaagaatgt aagtgtttta gatcgattaa
    25561 tagaaattgg ttcaaaagag gactatatta agggaagtcg tacacaggag tccttccgtg
    25621 aagtggtggc gaaaaatact ctttctggaa acttacatga cttcttgacc tacaatatga
    25681 aactattcac aaatgaaaca gacataaatg tttggtttaa gaaagctatt gaaaataatg
    25741 cttatgtagt tgaacaaccg tcaactaacc ctgcatttgc taataaaaaa tatcgtctat
    25801 atgaaggact taataacgga caacatggac gcatgatttt atcgcttctg aacttgaaag
    25861 atgcacattt atttatgatt tcaacatata atactatctc atttagttcg tttgaaaagt
    25921 atggtaaaga tacagaagaa aaacgtgaaa cgttcaagag tgaaatcaat aaacgtgcta
    25981 aagagcaagt taattactta gatttttggt cacgccttgc cactgataat gtccgcgata
    26041 aacttcttaa gagtcaaaat gtagttccaa ctcctgtatg ggataatcat ggtgcacccg
    26101 gtggttggcc tgatagattt ggtcatcgaa aagataaacc ggattatgca cctattcgtg
    26161 aattctttgg acgtattgat aagtatcatg attataagta tgggtatgga gcgtatgctt
    26221 acatctttgc tgatccacag cctatggatg ctgtatattt tgttatgtcg gatttaataa
    26281 gtgaatatgg cacatctgcc tttacacatg agactaccca cgtaaatgac cgaatggcat
    26341 atctgggagg tcatagacat cgacagggta cagatcttga agcatttgct caaggtatgt
    26401 tacaaactcc agcagaacat ggacatcaag gtgaatatgg cgcacttgga ttaaatatgg
    26461 catttgaacg ttcaaatgat ggaaaccaat ggtataatcc agatcctacc aaacttcaaa
    26521 cacgtgacca aatcgaccat tacatgaaga actacaatga agctatgatg atgctcgatt
    26581 atgcagaagc tgaggctgtt cttcctaagg ttaaaggtga caatagtaag tggttcaaga
    26641 aaattgatcg cgaaacacgt cgaccaatgg acagaaataa actaagtggt cctcaccaat
    26701 gggacaaagt gcgtgatttg atagacgctg agaaaacaac taaccttgaa accatcgatg
    26761 acttggtaaa taataatttc atgaccattc acggaaatcc aggtaataag gtcttccacc
    26821 cagaggactt tggtacggca tatgtcaatg ttaatatgat ggctggtatt tatggcggta
    26881 atacgagtca aggtgcccct ggttctctat ccttcaaaca taacgccttc cgtatgtggg
    26941 gttactatgg atatgaacat gggttcattg actatgtttc aagcaaacat caaggtgcag
    27001 ccaataaaga aaataacggt ctattaggtg atgatttcat tattaagaaa gtttctaata
    27061 accaattttc aaaccttgaa gattggaaga agcaatggta tggggaagtt cttacaaaat
    27121 ctaaaaaagg ttttgcacca attgaaatta atggcactca aatttcaacc tatgaacagt
    27181 tgaagactat gtttgatgag gctgttacta aagatttaac ggaactaaac gatagtagta
    27241 ttaaagacaa ataccgaaat acagttgtct tgaaatcgaa agtctttaaa gctctcctta
    27301 aaaacacaga cggtttctct ggtgatctat ttactgcacc acaagcatag aatgaataaa
    27361 atcgtaaaaa ggtagatcag ctttgtctgt atctgccttt ttatgctata cttaaggtat
    27421 gcatagaaaa acagtgattg attttaggac tttgggggag agatatacct ttaccaagcc
    27481 tattaaagag ttgaaaacga gaaatgtaga agaagtggca gatttgctgg cacaagtgga
    27541 aagctaccaa gagcaaggtt attatgtggt ggggtatgtc agctacgagg ctgcacctgc
    27601 ttttgaggaa aaattagcag ttcacaagga tcccctactg ggagagtacc tgctttattt
    27661 tactgttcat gatagcgtag aaacttcccc tattcctctg acttatgagg atattgattt
    27721 gccctcaaat tggcaggaag taacgtctgc agcagactat gaaaaggcca ttgcccaaat
    27781 tcaccatcat ttacggcagg gagataccta tcaggtcaac tacaccgtcc aacttaagca
    27841 aaagttaaat gccaatcctt ttgccatcta caatcgtatg gtggtagagc aggaggcggg
    27901 ctacaatgcc tatgtggaac atgacgagat ggcagtgatt tccatgagcc cagagctctt
    27961 ttttgagcaa aatgatcgcg agttgacaac acgaccaatg aaggggacga ctcagcgtgg
    28021 ggtaactgac caagaagatc ttgaacaggc cagttggttg gaacaggatc ccaaaaatcg
    28081 ctctgaaaat atgatgattg tggacctctt gcgcaatgat atgaaccgta tttctgaagt
    28141 tgggagcgag cacgtggagc gtctgtgtca ggtagagcag tattcaactg tttggcagat
    28201 gacttcgacc atcaagagtc agttgcgaga ggatgtggac cttgttgaaa tcttccgctc
    28261 actctttcct tgtggttcca taacgggtgc accgaaaatt gcgacaatgg agattatcaa
    28321 ggacttggag cctcaaccgc gtggagtcta ctgtggaacg attggtctct tgcttccaaa
    28381 tggacgacgg atttttaatg tggccattcg taccattcaa cttcacaaag gtcaagccat
    28441 ttatggagtt ggcggaggga ttacttggga tagcacatgg gaatctgaat accgagaggt
    28501 tcatcaaaag gcagcagttc tttatcgtaa acaagctcgc ttccaactga ttacgacagg
    28561 ggaaatcagc cagaagaacc tgttgtttga agatcaacat ctggaaagac tgagaaaagc
    28621 tagtcgttat tttgcctttc cttttgatgc agaagacttg ggacacaaga ttgaggaaga
    28681 gtgtcaggat tgtgaagcta atcgagatta ccgcttgcga atcagcctta gcaaatctgg
    28741 agagatagaa gtcaatcgtc aagtattaac ccctctcagt acaagctttt gtcaggccca
    28801 agtctgcctt caggaagctg ctttgaatca atcctttacc tactttaaaa ccactcaccg
    28861 accgcatttg agcctaggag aacaagagaa gatttaccac aataagtcag gagaactgct
    28921 tgaaacctct ataggaaatt tggttctgaa aatcgctgga aaactctaca caccgcctat
    28981 ccgacttgga atcttgccag gaatttaccg tcagtatttg ctagaaacag gacaggtaga
    29041 agagaaagtc ttgaccttgg cagatttagc ccaagcagaa gctatttacg gctgtaatgc
    29101 agtgagaggc ttgtatgaac taagccttga ggagaactag atgaaactga tatttttgca
    29161 tgggttaggg cagtcagcag agtcttggaa agaagttcgg aatctactga cagattatcc
    29221 ttctgaggct atcgagttat ttccttctgg agttagtaac tatcaacaag ctaaggagcg
    29281 agtttatcag cacctagctc aagagacaga accttttgtt ttgattggat tgtccttagg
    29341 tgctgccctg gcactagaac tttccagtta cgatttacca aatcttcgag ctttgattct
    29401 gtcaagatgc ccgcttaaat tagcagggaa tatcctattt tatcttcagt tgctgatttt
    29461 taaactactt cccaaaagag tatttgaaaa acagggagca gacaaggctc ttatggtcgg
    29521 agtttctgag gagttgaaga cacttgattt aacggatata gcaggaactt gcccttatcc
    29581 aaccttacta atttgtggta tcaaagataa accgaatctt agttctatga gaagtcttca
    29641 taaactaata tcagaatccc aatttcagat tatcccagat ggccctcatg tcttgaataa
    29701 ggcaaaacca aaggagtttg tagaaaagac cagaagtttc cttgaattgc tgaaataagt
    29761 ttgataaaat aatattgaaa tcgatatcat aattatggga gaaagaaatg aataaacgtc
    29821 tatttttaaa aatgagtctg gtgacgttgc caattttagc cttgttttca caaccagttt
    29881 tggcggaaga aaacatccat ttttcgagct gtaaggaagc ttgggcgaat ggctattcgg
    29941 atattcacga gggagaacct ggttattctg ccaagttaga ccgtgatcat gatggtgtgg
    30001 cttgcgaatt gaaaaatgct cctaagggtg cttttaaagc aaaacagtca acggctattc
    30061 aaatcaacac aagttcagca acaacaagtg gttgggttaa gcaggacggc gcttggtact
    30121 actttgatgg aaatggaaat ctagtgaaaa atgcatggca gggaagctat tacctgaaag
    30181 ctgatggtaa aatggcccag agtgaatgga tttatgactc ttcttatcaa gcttggtatt
    30241 atttgaaatc agatggttct tatgcaaaaa atgcatggca aggagcttat taccttaaat
    30301 caaacggtaa aatggcacaa ggtgagtggg tttatgattc ttcttaccaa gcatggtatt
    30361 acttgaaatc agatggttca tatgctcgca atgcatggca aggaaactac tatttgaaat
    30421 cagatggtaa aatggctaaa ggtgaatggg tttatgatgc cacctatcaa gcttggtatt
    30481 atttgacatc agatggttct tatgcttaca gtacatggca aggaaattac tatctaaaat
    30541 cggatggtaa aatggctgtc aatgaatggg ttgatggtgg acgttattat gttggcgctg
    30601 acggagtttg gaaggaaggt caagcaagta cagtttcttc tagtaatgat agcaatagtg
    30661 aatattctgc tgctttagga aaggcaaaaa gttataattc gttattccac atgtcaaaaa
    30721 aacgtatgta tagacaatta acttctgatt ttgataaatt ttcaaatgat gcagctcaat
    30781 atgccattga tcatttagat gattaacaga tactggtagg cgaaaaaatt ccttataatt
    30841 catttacgca aacctttgca taagtgaatg gattttgata tactagtact gtaagcattt
    30901 tcaattacta cacaatgaaa aaggagaata tgatgagtca aaagattatt gggattgacc
    30961 ttggtggaac ttctatcaaa tttgcaatct taacaacagc aggagaaatc caaggaaaat
    31021 ggtcaatcaa gaccaacatt ttggatgagg gaagtcatat cgttgatgat atgattgagt
    31081 ctattcaaca tcgtttggac ttgcttggat tggcagcagc ggacttccaa ggcattggaa
    31141 tgggatcacc aggtgtggtt gaccgtgaca aagggactgt tatcggtgcc tacaacttga
    31201 actggaaaac ccttcaacca atcaaacaaa agattgaaaa agctttgggc attccatttt
    31261 tcatcgataa tgatgccaac gtagcagctc ttggtgagcg ctggatgggt gctggagata
    31321 accaaccaga cgttgtcttt atgacactcg gtactggtgt tggtggcggt atcgtcgcag
    31381 aaggcaaatt gcttcacggt gttgctggtg cagcaggtga gcttggtcac atcactgttg
    31441 actttgacca gccaatctca tgtacttgtg gtaaaaaagg ctgccttgag acagttgctt
    31501 cagcaacagg gattgtcaac ttgactcgtc gctatgccga tgaatacgaa ggcgatgcag
    31561 ccttgaaacg cttgattgat aacggagaag aagtaactgc taagactgtc tttgatctcg
    31621 caaaagaagg agacgacctt gctttgattg tttaccgtaa cttctcacgt tacttgggaa
    31681 tcgcttgtgc taacattggt tcaatcctaa acccatcaac aatcgtcatc ggtggtggtg
    31741 tgtcagctgc gggagaattc cttctacaag gtgttcaaaa agtttacgat gaaaatagtt
    31801 tcccacaagt acgcacatct actaaattag ctcttgcaac tctaggaaat gacgctggag
    31861 ttatcggagc agcatcactt gtattgcaat aagataatga gagaggaaaa tgctaaccta
    31921 gagttagtaa tgttcctctt ttatttttta tgctatacta gttaggacaa tgaatgaggt
    31981 gagagtaaat gacaaaagca gatacgattt ttaaagagaa tattgaacgc attctaaagg
    32041 aaggtgtttt ctcagagcag gctcgtccta agtacaagga tgggactgtt gctaattcca
    32101 agtatgtaac tggtgccttt tccgagtacg acttatctaa aggagaattt cctatcacaa
    32161 ccttgcgtcc cattgcaatc aaatccgcca tcaaagaagt tctctggatt taccaagacc
    32221 agtcaaatag cttagaagtg cttaatgaca agtacaatgt tcactactgg aatgactggg
    32281 aagttggaga cacgggaacc attggtgagc gctatggtgc cgttgttaag aaacacgaca
    32341 ttatcaataa gcttctcaaa cagttggaaa ccaatccttg gaaccgccgc aatattattt
    32401 cgctctggga ttaccaagct ttcgaagaaa cagatgggct gctcccgtgc gcctttcaga
    32461 ccatgtttga tgtccggcgt gttgatgggg aaatctatct ggatgcgacc ttgacccagc
    32521 gctccaatga tatgctggtg gcccaccaca tcaacgctat gcagtatgtg gctttgcaga
    32581 tgatgattgc caaacatttt ggctggaagg ttgggaagtt cttctacttc atcaacaacc
    32641 tccatatcta tgataatcaa tttgaacaag ctcaggaatt gctccgtcgg gagccgtcaa
    32701 actgccaacc acgcttggtt ttaaatgttc ctgatgggac taatttcttt gatatcaaag
    32761 cagaagattt tgagttggtg gattatgacc ctgttaagcc acagttgaag tttgacctag
    32821 ctatttaaaa gaatagaaaa aagaagttga gaataatccc aacttctttt gtttcttaac
    32881 gtgatacgcg gcgacgagct gcttttttac ggttttcttc gatgaaagct gctttttgct
    32941 cttctggttc gattactttc tttttaaatg cgtatactgc acctgcaacg gcagcgacag
    33001 ttcctgcgac acctgttaca agacctttag cgaatccttt agccatgagt cttcctcctt
    33061 tatattctca atcagccagc ctcctcaaga ggtcacattt ttctgactga cctttttgtg
    33121 ttataataat agtaacgaaa aaatgggaat ttttcaagga aaaaagatga aaacaaaaat
    33181 aattgtgatt gttggaccga ctgctgttgg aaagacggct ctagctattg aagttgcaaa
    33241 gcgttttaat ggcgaagtgg ttagtggaga tagccagcaa gtctatcgag gacttgatat
    33301 tgggacggcc aaggctagtc cagaagagca ggcagctgtt cctcatcatt taatcgatgt
    33361 tagagagata accgagtctt actcggcttt tgattttgtt tcagaagcta agatgactat
    33421 tgagggtatt cacaaccgtg gcaagctagc cattatcgcc ggtgggactg gactttatat
    33481 ccagagcttg ctagaaggtt accacctagg tggggagact cctcatgagg agattttagc
    33541 ttatcgagct agtttggagc catattcaga tgaggaatta gcccatttgg tggatcaagc
    33601 aggccttgag attccccagt ttaatcgtcg tcgtgctatg cgtgccttag aaattgccca
    33661 ttttggtcag gatttggaaa atcaagagac cttgtatgaa ccgctgatta tctgcttgga
    33721 tgatgaacgt agtcaacttt atgagcgtat caaccaccga gtggatttga tgtttgaggc
    33781 tgggcttttg gatgaggcca agtggctgtt tgaccattcc cctaatgtac aggctgctaa
    33841 aggaattggc tataaggaac tctttcctta tttccgtgga gagcagacct tggaggaagc
    33901 tcgtgagagt ctcaaacagg ctactcgtcg ttttgccaag cgtcagctga cctggttccg
    33961 taatcgcatg caggtcacct tttatcagat tggagaatct ggtgtacaag accgcatttt
    34021 aagccagata gaggagtttt tagatgattg aaacggagaa aaaagaggag cgggtcctgc
    34081 tgattggtgt ggaattgcag ggtatggaca gttttgacct ctccatggaa gaattggcta
    34141 gtttagcgaa aacggcaggg gcagtcgttg tagatagcta cagacaaaaa cgtgaaaaat
    34201 atgattccaa gaccttcgtc ggctctggta agttggaaga gattgcgctt atggtggatg
    34261 cagaagaaat cactactgtc atcgtcaaca accgtctgac cccaaggcag aatgtcaatc
    34321 tagaggaagt tctcggtgtt aaggtcattg accgtatgca gttgattttg gatatctttg
    34381 ccatgcgggc tcgaagccat gaagggaagc tccaagtcca cctagcccaa ctcaaatacc
    34441 tcttgcctcg cttggttggt caggggatta tgctcagccg tcaggcaggg ggaattggtt
    34501 cccgtggtcc tggtgaaagc caactggagc tgaaccgtcg tagcgttcgc aatcaaatca
    34561 cggatatcga gcgccagctt aaggtggttg agaaaaatcg tgcgactgtc agagaaaaac
    34621 gtttggagtc tagcaccttt aagattggtt tgattggtta taccaatgct gggaaatcaa
    34681 ctatcatgaa catcttgacc agtaagaccc agtatgaagc agatgagctc tttgcgactc
    34741 tggatgcgac aaccaagagt attcatctag gaggcaatct ccaagtaact ttgacagata
    34801 ccgttggctt tatccaagat ttgccgacag agttggtgtc cagtttcaag tcaaccttgg
    34861 aagaaagcaa gcatgtggac cttctggttc atgttatcga tgctagcaat ccttaccacg
    34921 aggagcatga aaaaacggtt ctctccatca tgaaagacct ggacatggaa gatattcctc
    34981 acttgacgct ttataataaa gcggatttgg tggaggattt cacgcctacc caaacgccat
    35041 ataccctcat ttctgccaag tctgaggaca gtcgtgaaaa cttgcaagca ttattgctag
    35101 ataagattaa ggaaattttt gaagcattta ccctgcgagt gcctttttca aagtcctaca
    35161 agattcatga tttagagagt gttgcgattc ttgaagaacg tgattatcag gaagacagcg
    35221 aagtgattac aggctacatt tccgagaaaa ataaatggag gttagaagaa ttttatgact
    35281 gatattaaaa ctttggctct aaagtatgga ggttatacaa gtctggacaa ggtctatctg
    35341 gatcagcttc tagctggtaa aacagagcag gagcagttaa cacttattac acctccgcca
    35401 agtgttgtta atgcctactt tgcagaactc tatcagaaaa agagtcctca ggctgcgacg
    35461 gattattttg cagaactcag tcaggaactg aatctttaca atactgagcc aagtttcacc
    35521 ctagaaaata agtcttttat tcggcttaat ctgtctggta aatcctttgg tttttgttat
    35581 gagagtgacg gctttggtcg gattttctct gaaaatgagg agaaaatctc ggatgacttg
    35641 ctctttgaaa ttgcgcaaat tttcccccat caattagtct ttgaagagtc tggtaagatt
    35701 tacatgaagc ctgtcgagga cgaggaagtt gtcagcctag aaagtctcac agctttgact
    35761 gatttggaaa gattggctga tggtcgaaaa cgtctcaaag gctacagcca agaggattta
    35821 ttgaaagaag ctgctgcttt ttctggcaaa cgctatttcc gatcggaaaa ccgcacagcc
    35881 atgttatata ttgattaatt agaaagtatc gaatggatat tcaattttta ggaacggggg
    35941 ctggtcagcc ctctaaagcc cgcaacgttt caagtctcgc cctgaaactt ttggatgaga
    36001 ttaacgaagt ttggctcttt gactgtggag aaggtacgca aaatcgcatt ctggaaacca
    36061 caattcgacc acgtaaggtc agcaaaatct ttattaccca tctgcatgga gaccacattt
    36121 ttggtttgcc aggtttcctt tctagccgtg cctttcaggc caatgaagag cagacagatt
    36181 tggaaatcta cggacctcaa ggaatcaagt catttgtctt aaccagcctt cgtgtgtcag
    36241 gttctcgtct gccctaccgc attcatttcc atgagtttga ccaagattct ctgggtaaaa
    36301 ttcttgaaac cgataaattc actgtgtatg cagaggagct ggaccacact attttctgtg
    36361 ttggctatcg tgtcatgcaa aaggatctag aagggacgct ggatgctaaa aaactcaagg
    36421 ctgctggtgt tccgttcggc ccgctttttg gtaaaatcaa aaacggccag gatcttgttt
    36481 tggaagacgg aactgaaatc aaggcagcag actatatctc agcgccacgt ccaggtaaga
    36541 ttatcactat tttaggagac actcgaaaaa cggatgccag tgtgcgtctg gctgtcaatg
    36601 cagatgtcct agttcatgag tccacttatg gcaaggatga tgaaaaaatt gctcgtaacc
    36661 atggtcactc aactaatatg caagctgcac aagtagcggt agaagcaggt gccaaacgcc
    36721 tcctactcaa ccatatcagt gcccgtttcc tctcaaaaga tataagcaaa ctcaagaaag
    36781 acgctgccac aatttttgaa aatgtccatg tggtcaaaga cttggaagaa gtggaaatct
    36841 agcagtcaca gaaaggataa gtatgcctac tattctcatt accggagcta gcggtggtct
    36901 agctcaagaa atggtcaaac tcctgcccaa tgaccaactc atcttgcttg gtagaaataa
    36961 ggaaaaatta gcccaactct acggaaatta ttcccatgca gaattgattg aaattgatat
    37021 taccgacgat tcagccctag aagctctggt aactgatctt tatctccgct atggcaagat
    37081 tgatgtcttg attaacaacg ctggttacgg gatttttgag ggatttgacc aaattgctga
    37141 taaagacatt caccagatgt ttgaggtcaa tacctttgcc ctgatgaatc tgtctcgtca
    37201 ccttgcggct cgtatgaagg aaagcagcaa agggcatatc atcaacatcg tcagcatggc
    37261 aggtctaata gctactggca agtctagtct ttactcagcg accaagtttg cggctattgg
    37321 tttttcaaat gctctgcgac tcgaacttat gccctatgga gtctatgtga caacagtcaa
    37381 tccaggtcca atccgaacag gattttttga ccaagctgac ccagatggaa cttatcttaa
    37441 atcggttgac cgcttcctct tagaggcaga tgcagtggct aaaaagattg tcaagattat
    37501 aggcaaaaat aaacgagaac tcaatctccc gattttgttg aacctagccc ataagtttta
    37561 tactctcttt cccaagctag ctgataagtt ggcaggggaa acttttaatt ataagtaaaa
    37621 agaaccaatg tgcaggttgt tgctagccta catattggtt cttatcattt ctcaactatc
    37681 aaactgaact tcttctagta gatagtcgat aaagcgttcg cccatcttag ataggctggt
    37741 tttctcatgc tggatataga ccagctcaat cgggtcatca atatccagtg ggatagaaac
    37801 gatattgtct ccgtttaggt tgctgttcaa aatccctgtc gcaatggtat aaccatccaa
    37861 accaatcaag agattaaaga gggtggcacg gtcactgacc acaatggatt tcttgtggtg
    37921 ttcttgagaa agaatctctt ctgaaaagta gaaggagttg tgcgtccctt ggtcatagct
    37981 gaggtaaggg aaattctcca aatcagacag tttcaccttg tctttctttg ccagagggtt
    38041 ggtcttgctg acaaagatat gcggttgcgc tgtgaagaga tggtgggcta gcaggtgatt
    38101 gtcatccagc atcttggtta aaacatcacg gttgtaactg tttaagaaga ggaccccgac
    38161 ctcactgcgg aagttcttga cgtcgtcgat aatctcccaa gtccgagttt cacgaaggaa
    38221 gagttcgtat ttctccatat cgcttttctt gagcaaagag acaaaggcat tgaccacaaa
    38281 ggcatagtgt tgagacgaaa cgctaaagag ttcgcggtgg gcgacaggat ttttatagcg
    38341 ttcctccaga agctgggtct gctcgacaac ctgacgggca taagagagaa actccatccc
    38401 atcacgggtc aaggtgattc ccttgggatt gcggataaag atctcaatgc ccatttcatt
    38461 ttccaaatct cgcactgcat tggagagact tggctgagtg ataaagagtt gcttggctgc
    38521 ctcattcatg gagccagttt cgacgatttt gataatataa tgtaattgtt gaattctcat
    38581 gtttttattg taccatactt gccttagaat cgctaaagaa gataggaaaa taaggagcat
    38641 tgtgtgagtc tcttcttgaa aaaaacacaa aattttgcta gaatgagtct tatgaaaaca
    38701 ttctatgatg tgcagcaatt cctcaaacga tttggtatta ttgtttacat gggaaaacgc
    38761 ttatatgata ttgaactgat gaagttggaa ctatctcgga tttacgatgc agggttgatg
    38821 gataaattag actatctaga ggcagaagca gttcttcgca gagagcacaa ggtagaattg
    38881 gattatattg agaaaaatgg agaaaagaac taatggttac ttggattttg tgggcactta
    38941 tactagcaat gttggcgtgg atgggcttta actatcttcg tattcgccgt gcggctaaaa
    39001 ttgtggacaa tgaggagttt gaagccttga ttcgtacggg tcaattgatt gatttgcgcg
    39061 acccagcaga attccacaga aaacatatcc ttggtgcacg caatattcct tcaagtcagt
    39121 tgaaaactag tcttgcagcc cttcgtaaag ataaacctgt ccttctctac gaaaaccaac
    39181 gtgcgcaacg agttacaaat gcagctcttt acttgaaaaa acaaggtttt tctgagattt
    39241 atatcctttc ttatggcttg gattcttgga aagggaaagt gaagactagc taaccttatt
    39301 tccaaattag tcgaatgata ggatgaaga
//
LOCUS       DBERCA010000014        34653 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.014, whole genome shotgun sequence.
ACCESSION   DBERCA010000014 DBERCA010000000
VERSION     DBERCA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.014"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            249..2141
                     /gene="pepO"
                     /locus_tag="ACL719_000825"
     CDS             249..2141
                     /gene="pepO"
                     /locus_tag="ACL719_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGS0698829.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2263..2892)
                     /locus_tag="ACL719_000826"
     CDS             complement(2263..2892)
                     /locus_tag="ACL719_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS0698830.1"
                     /translation="MKIYKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3886..6108
                     /locus_tag="ACL719_000827"
     CDS             3886..6108
                     /locus_tag="ACL719_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HGS0698831.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6138..6581
                     /gene="dtd"
                     /locus_tag="ACL719_000828"
     CDS             6138..6581
                     /gene="dtd"
                     /locus_tag="ACL719_000828"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGS0698832.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6848..7033)
                     /locus_tag="ACL719_000829"
     CDS             complement(6848..7033)
                     /locus_tag="ACL719_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698833.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(7052..7465)
                     /locus_tag="ACL719_000830"
     CDS             complement(7052..7465)
                     /locus_tag="ACL719_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698834.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7596..8303)
                     /locus_tag="ACL719_000831"
     CDS             complement(7596..8303)
                     /locus_tag="ACL719_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HGS0698835.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGL
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQISQKQVKLAVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            complement(8422..9072)
                     /locus_tag="ACL719_000832"
     CDS             complement(8422..9072)
                     /locus_tag="ACL719_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGS0698836.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMVIAKQLYVEKIN"
     gene            9184..10026
                     /locus_tag="ACL719_000833"
     CDS             9184..10026
                     /locus_tag="ACL719_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0698837.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQLHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10102..10539
                     /locus_tag="ACL719_000834"
     CDS             10102..10539
                     /locus_tag="ACL719_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGS0698838.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10608..10910
                     /locus_tag="ACL719_000835"
     CDS             10608..10910
                     /locus_tag="ACL719_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698839.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSFISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(11001..12074)
                     /locus_tag="ACL719_000836"
     CDS             complement(11001..12074)
                     /locus_tag="ACL719_000836"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGS0698840.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPSQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPK
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            12230..12907
                     /locus_tag="ACL719_000837"
     CDS             12230..12907
                     /locus_tag="ACL719_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0698841.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12900..13874
                     /locus_tag="ACL719_000838"
     CDS             12900..13874
                     /locus_tag="ACL719_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGS0698842.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13923..15866)
                     /gene="thrS"
                     /locus_tag="ACL719_000839"
     CDS             complement(13923..15866)
                     /gene="thrS"
                     /locus_tag="ACL719_000839"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGS0698843.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15924..16247)
                     /locus_tag="ACL719_000840"
                     /pseudo
     CDS             complement(15924..16247)
                     /locus_tag="ACL719_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            complement(16504..16867)
                     /locus_tag="ACL719_000841"
                     /pseudo
     CDS             complement(16504..16867)
                     /locus_tag="ACL719_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17104..17733
                     /locus_tag="ACL719_000842"
     CDS             17104..17733
                     /locus_tag="ACL719_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0698844.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17874..18143
                     /gene="rpsO"
                     /locus_tag="ACL719_000843"
     CDS             17874..18143
                     /gene="rpsO"
                     /locus_tag="ACL719_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGS0698845.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18958..19572
                     /locus_tag="ACL719_000844"
     CDS             18958..19572
                     /locus_tag="ACL719_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGS0698846.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19865..20614)
                     /locus_tag="ACL719_000845"
     CDS             complement(19865..20614)
                     /locus_tag="ACL719_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGS0698847.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20747..23083
                     /locus_tag="ACL719_000846"
     CDS             20747..23083
                     /locus_tag="ACL719_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS0698848.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23185..23862
                     /locus_tag="ACL719_000847"
     CDS             23185..23862
                     /locus_tag="ACL719_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGS0698849.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            23849..24646
                     /locus_tag="ACL719_000848"
     CDS             23849..24646
                     /locus_tag="ACL719_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGS0698850.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24663..25766
                     /locus_tag="ACL719_000849"
     CDS             24663..25766
                     /locus_tag="ACL719_000849"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS0698851.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            26022..27041
                     /gene="galE"
                     /locus_tag="ACL719_000850"
     CDS             26022..27041
                     /gene="galE"
                     /locus_tag="ACL719_000850"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS0698852.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKVMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            27043..28008
                     /locus_tag="ACL719_000851"
     CDS             27043..28008
                     /locus_tag="ACL719_000851"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0698853.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAAHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(28059..28265)
                     /locus_tag="ACL719_000852"
     CDS             complement(28059..28265)
                     /locus_tag="ACL719_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGS0698854.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28317..28793
                     /locus_tag="ACL719_000853"
     CDS             28317..28793
                     /locus_tag="ACL719_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGS0698855.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSELESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28802..29473
                     /gene="cmk"
                     /locus_tag="ACL719_000854"
     CDS             28802..29473
                     /gene="cmk"
                     /locus_tag="ACL719_000854"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGS0698856.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29604..29942
                     /locus_tag="ACL719_000855"
     CDS             29604..29942
                     /locus_tag="ACL719_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGS0698857.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29945..30574
                     /locus_tag="ACL719_000856"
     CDS             29945..30574
                     /locus_tag="ACL719_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGS0698858.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30814..31965
                     /locus_tag="ACL719_000857"
     CDS             30814..31965
                     /locus_tag="ACL719_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0698859.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32278..33027
                     /gene="truA"
                     /locus_tag="ACL719_000858"
     CDS             32278..33027
                     /gene="truA"
                     /locus_tag="ACL719_000858"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGS0698860.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRKLAGPTAAPNGLYLKEIRYEE"
     gene            33017..33790
                     /locus_tag="ACL719_000859"
     CDS             33017..33790
                     /locus_tag="ACL719_000859"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS0698861.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33768..34229
                     /locus_tag="ACL719_000860"
     CDS             33768..34229
                     /locus_tag="ACL719_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0698862.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            34454..34653
                     /locus_tag="ACL719_000861"
                     /pseudo
     CDS             34454..34653
                     /locus_tag="ACL719_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10039 a         6486 c         7910 g        10218 t
ORIGIN      
        1 aattaagtca agttaatttt tgaaaaaatt aaaataataa ctgaaaaata gattctaaag
       61 ataactttca ggataaattt ctaaattata aaacgcatag tatcaagtgt aaaaaacttg
      121 gaattatgcg ttttatcatg gaaagatttt ttataatagc taaaaaataa tagttaaatg
      181 gttattaatt gcattcccta gtgatttttg ttaagataaa tgcaaataca aatgaaagcg
      241 agaacaagat gacacgttat caagatgatt tttatgatgc tatcaatgga gaatggcaac
      301 agacagctga aatcccagca gataagtctc aaactggagg ttttgttgat ttagaccagg
      361 aaattgaaga cctgatgctg gcgacaacag acaagtggtt agcaggtgaa gaggtgcctg
      421 aggatgctat cttggaaaac tttgtcaaat accaccgcct agttcgtgat tttgacaaga
      481 gagaagctga cggtatcaca cctgtcttac cactccttaa agaattccaa gaattggaaa
      541 cttttgcgga ttttacagct aaactagcag agtttgagct tgcaggaaaa ccaaacttcc
      601 ttccttttgg tgtatcgcca gactttatgg atgctagaat caatgttcta tgggctagcg
      661 ctccaagcac aatcttgcca gatacgacct actatgcaga agaacatcct cagcgcgaag
      721 aactcttgac tctttggaaa gaaagcagcg caaatctcct caaggcttat gatttctctg
      781 atgaagaaat tgaagacttg ctagaaaaaa gacttgaatt ggaccgccga gttgcggcag
      841 tggtgctctc taatgaagaa agttcagaat atgctaaact ctatcatcca tattcttacg
      901 aagatttcaa gaaattcgcg cctgccctac ctttggatga cttcttcaaa gcagttattg
      961 ggcaattacc agacaaggtt attgtagacg aggaacgttt ctggcaagca gcagagcaat
     1021 tctacagtga ggaagcctgg tctctcctta aagcaacctt gattttgagt gttgtcaatc
     1081 tttcaaccag ctatttaaca gaggatatcc gtgttttgtc tggtgcctac agccgtgccc
     1141 tttctggagt tccagaggca aaagataagg tcaaagcagc ttatcatcta gcacaagaac
     1201 ctttcaagca agccctgggt ctttggtacg cccgtgagaa gttctctcca gaagccaagg
     1261 cggatgtgga gaaaaaagtg gcaaccatga ttgatgttta taaggagcgt ctgcttaaga
     1321 atgactggct cactccagaa acctgtaaac aggctatcgt gaagctcaat gtgatcaaac
     1381 cttatattgg ctatccagaa gaattgcctg cacgttacaa ggataaggta gtgaatgaaa
     1441 ctgccagtct ttttgagaat gctctagcct ttgcgcgtgt ggaaatcaag cacagttgga
     1501 gtaagtggaa ccagcctgta gattataagg aatggggcat gcctgctcat atggtcaatg
     1561 cctactacaa tcctcagaag aacctgattg tctttccagc ggccatttta caggcgcctt
     1621 tctatgactt gcatcagtca tcttctgcta actacggtgg tattggggca gtgattgccc
     1681 atgaaatttc ccacgccttt gatactaacg gggcttcctt tgacgaaaat ggtagcctca
     1741 aggattggtg gacagagagc gactatgctg ccttcaagga gaaaacacaa aaagtcattg
     1801 accaatttga tggacaggat tcttatggag caaccattaa cggtaaattg actgtatcag
     1861 aaaacgtggc tgacttggga ggaatcgcag cagcgcttga agcagctaag agagaagcag
     1921 acttctcagc agaagagttc ttctacaact tcggtcgcat ctggcgcatg aaaggtcgac
     1981 cagaatttat gaaacttttg gctagcgtcg atgtgcacgc accagccaaa ctccgtgtca
     2041 atgtgcaagt accaaacttc gacgatttct ttacaaccta tgatgtcaaa gaaggagacg
     2101 gaatgtggcg ttcaccagag gagcgcgtga ttatttggta atacaaaaaa tctagtcatc
     2161 agaaaaaggc atccaatcac gtgtgaaaac cttgatagga tgccttttgt aatagaaaga
     2221 tttgctggat agtttactta aatttcgatt gtcatcatct tgctaaaaaa agggattgaa
     2281 ggccttttcg tgagcgatag tagtagctgg accatgtcct ggatagacat cgtagtttgg
     2341 tagggtgaag agttgggtct ggatactatg aaggagttgc tccatgctac cagtcggaag
     2401 gtcggtccgt ccgatagttt cgcggaatag agcatctccc gtcaagacta ggtgagcatc
     2461 aggaaagact agggaaacac caccgataga gtgccctggg gtcggtagaa ccttaaaacg
     2521 aaattcctct agttggtatt cttcgtgaaa gacaaaggtg tgttctgcag gttttgtgac
     2581 cacatctgcc atatcatcgt ggcgagggag accggagaga ttatcgacag gagtgtagag
     2641 ccagctggct tcgctctctg cgatatagac aggaggattg ccaaacgtct cgcgaaccaa
     2701 gtccagactc atgatatggt cataatgggc gtgggtcaag agaatagcac agatcggttt
     2761 gttgatcttc tcgattgtct gacgaatggc ttcccaatga ctaccaggat cgacgacgat
     2821 gaggtgcttt tcgccttcta gataataggt attttcatag gcaacaggat tcacggtttt
     2881 atagattttc atactagctc caatctcaaa gaatatacta atactcttcg aaaatctctt
     2941 caaaccgcgt caactttatc tgcaacctca aagcagtgct ttgaacagcc tgcggctagc
     3001 tttctagttt gctctttgat tttcattgag tataaattaa gtatagcaca gttagggaga
     3061 ataggtaagg atttaaaatg aacttaaaaa ccagcatgac tagatgaaga caggctggtt
     3121 atcaatttat caatattgga aaggtgtcaa ttgcccttca taggttgcat aaactccgtc
     3181 attagcttgt ttgatagacg tgatgtttag agtgctaccg tagttggctt ctcatttgtc
     3241 gctatattca ctataagtta tcctactggg caggttctgg tcgttagcat agtattgaac
     3301 gagtttttta tagctttgct ggctgaatag gaacaggcag caaactccta acattaggag
     3361 agcaaagata gaaatagcag tttttttcat aggtattctc cttataaact aaaggataga
     3421 agaaagtatc ttgaggattt ttatactctt caaaaatctc ttcaaaccgc gtcaacgtcg
     3481 ccttgccgta tatatgtgac tgactttgtc agtcttatct acaacctcaa agcagtgctt
     3541 tgagcaacct gcggctagtt tcctagtttg ctctttgatt ttcattgagt attaattcca
     3601 aaatccagtc aaggtaccag tgtatgtgat acgagttcca ccgtttatct ttcgtatctt
     3661 caatggaatt ggggaaatca gaaagtcttt gggttacaat atggtttccg ttcttctatc
     3721 tctccttcta tcacctaagt ttaaaatcta acttcgtagt atattatggg ccaataattc
     3781 agaattatca agcaattttg aattattttt ttcttattct gttccaatct agttttcata
     3841 ttcttaaaaa agtgataaaa tggtaggaag aattggagag tagagatgcc gaaagaagtg
     3901 aatttaacag gcgaagaagt tgtcgcttta accaaagaat atttaacgga agaggatgtt
     3961 cattttgtcc ataaggcctt ggtctatgct gttgaatgcc acagtggtca atatcgcaaa
     4021 tcaggcgagc cttatatcat tcaccctatc caagtggcag gtattttagc taagctaaag
     4081 ctggatgctg taacagtagc ttgtggattc ttgcatgatg tggtggaaga tacagatgcg
     4141 accttggacg atttggaaag agagtttggt cctgatgtgc gggtgattgt tgacggagtt
     4201 accaagcttg gcaaggtcga gtacaaatcg atcgaggagc aattagcgga aaatcatcgc
     4261 aagatgctca tggccatgtc tgaggacatc cgcgttattt tggtcaaact gtctgaccgc
     4321 ttgcacaata tgcggaccct gaaacatctt cgaaaagaca agcaggagcg tatttccaaa
     4381 gaaaccatgg aaatctatgc cccacttgcc catcgtttgg ggatttccag tgtcaaatgg
     4441 gaattagaag acttgtcttt ccgttatctc aatccaacgg agttttacaa gattacccat
     4501 atgatgaagg aaaagcgcag ggagcgtgag gccttggtgg atgaggtagt cacaaaatta
     4561 gaggagtata cgacagaacg tcacttgaaa gggaagattt atggtcgtcc caagcatatt
     4621 tactcaattt tccgcaaaat gcaggacaag agaaaacggt ttgaggaaat ctatgatctg
     4681 attgctattc gttgtatttt agatacccaa agtgatgttt atgccatgct tggttacgtg
     4741 catgaatttt ggaaaccgat gccaggtcgc ttcaaagact atattgccaa ccgcaaggcc
     4801 aatggttatc agtctatcca tacgactgtt tatggaccaa aagggccgat tgaattccag
     4861 attcgaacca aggaaatgca cgaggtggct gagtacgggg ttgcggctca ctgggcttat
     4921 aagaaaggta taaaggggca agttaacagc aaggaatcag ctattggaat gaactggatc
     4981 aaggagatga tggagctcca agaccaggct gatgatgcta aggaatttgt ggactctgtt
     5041 aaggaaaact atttggctga ggagatttac gtttttaccc cagatggagc tgtccgttcc
     5101 cttcccaaag attcaggacc gattgatttt gcctacgaaa tccataccaa ggtcggtgaa
     5161 aaagcaactg gtgccaaggt caatggccgc atggttccac tgacaaccaa gttaaagaca
     5221 ggggatcagg ttgaaattat cgccaacccg aactcctttg gacctagccg tgactggctc
     5281 aatatggtca agactagcaa ggcgcgcaat aagattcgcc agttctttaa aaaccaagat
     5341 aaggaattgt ctgtcaacaa gggtcgtgag atgctgatgg ctcagttcca agaaaatggc
     5401 tatgtggcaa ataaatttat ggacaagcgc cacatggatc aagttctgca aaagaccagt
     5461 tacaagacag aagactccct ctttgcggcc attggttttg gggaaatcgg tgcgattacc
     5521 gtctttaatc gtctgactga aaaggaacgc cgtgaggaag agcgtgccaa ggccaaggct
     5581 gaggcagagg agcttgtcaa aggtggcgag gtcaaggttg aaaataaaga aactctcaag
     5641 gtcaagcatg aggggggagt ggttattgaa ggtgcttctg gtctcctagt gcggattgct
     5701 aagtgttgta accccgtgcc tggtgacgat attgttggct acattaccaa gggtcgtggt
     5761 gtggctattc accgtgtgga ctgtatgaac ctgcgtgccc aagaaaacta cgagcaacgt
     5821 ctccttgatg tggaatggga agaccagtac tctagctcaa ataaggagta tatggcccat
     5881 atcgatatct acggtctcaa ccgtacagga ctgttgaacg atgtactgca agttctttca
     5941 aatacaacca agaatatttc aacggtcaat gcccaaccaa ccaaggatat gaagtttgct
     6001 aatatccatg tgtccttcgg tattgccaac ctctctacac tgaccacggt tgtcgataaa
     6061 attaagagtg tgccagaagt ttactctgtc aaacggacca acggctagtt gtcctagctc
     6121 ttactagaaa ggctattatg aaaatcatta tccaacgggt taaaaaagcc caagtgagta
     6181 tagaaggcca gattcaggga aaaatcaatc agggactttt attgctggtt ggtgttggac
     6241 cagaggacca agaggaagat ttggactatg ctgtgagaaa actggtcaat atgcggattt
     6301 tttcagacgc agaaggcaag atgaacctgt ctgtcaaaga tattgaagga gaaatcctct
     6361 ctatttctca gtttaccctc tttgcggata ctaagaaagg caatcgtcca gcctttacag
     6421 gggcagctaa acctgatatg gcatcagact tctatgatgc tttcaatcaa aaattagcgc
     6481 aagaagtgcc cgttcagaca ggtatctttg gagcagatat gcaggttgag ctggttaata
     6541 acggacctgt taccattatc ctagatacta aaaagagata agaaagacca agcccagtcg
     6601 gcttggtctt tctcatctat cataaaatac tccaaaaaga aatcggttct tgatatgctt
     6661 gggggaagtc tcttttcagg ctttggcaga tgcgatagga agggatgaga tgtcctagga
     6721 tgaggagagt tccctgaagg aaaagtggaa tatcacttaa agacaccaag gagagaatca
     6781 tcaggctatc ccataggaga atttgtaaag caaaacgcca gaatctcggt ctaatctggg
     6841 aatagagtta actaaagtgg tcacaaagtt ggtttgaaag ataggtcaat agcacaggat
     6901 gaaagaaaat gagccaaccg ctataaatca aaatccagtc ccaagtaagc ccccttttaa
     6961 atgtcaaaaa tgccagcatg attccatcaa tgacaaggag ttgaatggtt ttgatgaaga
     7021 tgattttttt catgctaaaa cctccttttc cttagagaca ctcccataaa gagatgtgtt
     7081 tgtcgtaaaa gtctggatag ttttctctta ggtagtcagc tgtcatataa taaaaagtag
     7141 aatggcaagc agtgataatt gacataagag atgaaaaacg ctgagaacta atgaaggcta
     7201 agagaacaaa aaatagaaaa tcaatggtga gaacccctaa atagtagaag cgaatctttt
     7261 tattgatttg aggataaatc tcagcgaatt gatgtttgag tcggacgatc aagagaaata
     7321 gcaggagtcc ttgagcaagg aagaaaatta aaccagagag caagagccac tcaaaagatg
     7381 tctcgagtct aatgataaaa aatgcaaata gtagcaaatc aataactgca attgctataa
     7441 aatggattct atagagtttt ttcatgacaa gacctccctc ttttatctaa cttcattcta
     7501 ctccaaaaga atgggagtta caactaaaat gataaaaata gcagaaggga gattctctta
     7561 agttggctag tattctttat ttgagtttcc ttctattatc taacttcttc atcattccag
     7621 acaaataaag ctccgattgc attgaggata taaaagatgt atttaccgat attggcgaag
     7681 tttccttgaa taccagcttt tgtcagctga acgaaattgt aaatcaacca aaagccccac
     7741 tgagttgtta gttttaatgc attcaaagca ttggcaatga gggatagtgc aaaggcaata
     7801 gttgttacgt aggcaaggag attcatcttg ccctcatatc cgatatagtt ggtcacaaag
     7861 gcaaagagga aggcgatgat ggaaatgatg atgaccgcca attttacctg tttttggctt
     7921 atttggttgg gtctgccttc ttgcgaagct ttccatttct ttatagcaaa ggtataaatg
     7981 aggaaggtga cgggataggt aatgatggcc gccttatttc caaggatata atcaatagca
     8041 ccggacaaaa tggtattaac aataccaaag taatttcccc atttgcttaa tttccccgtg
     8101 aaacgagtgg acaacatgga aatcccaacg ttggttacgg aaatcaatcc aaagggtaca
     8161 agagctgtcc atagtcccca gtctacaaat ttatcgaggc gtgagttgag ataaccagat
     8221 gcaatcgcaa tcccaacgac caaagcaacc ccgaagaggt caaaccattt agatgtagca
     8281 aaaattttta gtgatttttt cataggttaa actacctttc tttttttttc aaatattcta
     8341 ccaccaaatg aaagtaaaat aaaatgatag aaataaaacc ctgaaaataa aactttcagg
     8401 gttggtaggg gacttgagaa attagttgat tttctcgaca tagagttgtt ttgcaatgac
     8461 catattcact tgtaaatcct cgtcgttact gaggatagtg aaggtattgc tgaagccatc
     8521 aaactgcttg acttggagct ggtcaccgat gtgaagtgag tgcttgtcca gataatggag
     8581 aatgtcaaaa ctatcgtgca cccgagtcag gcggtaggcg ccagcttcct tgatatcagc
     8641 tagtgggagg ttattgattt caacgaggag ttctcccttg gcaggaatag ttcctccgtg
     8701 ggggcaggtt ttagggaaac ctagcagttt atctagtctt tccacgaaca ggtcagagac
     8761 agtgtgttcc aagacctcag cttcctcgtg aatctggtca cttgtatagt ctaaatgatg
     8821 aactagaaaa acttcaatca agcggtgctt acgatagagc tcagagacca gtttgagacc
     8881 gaggtcagtc agtagatagc cacattcctt gtcctttagg atgagatttt cacttttcat
     8941 tcgtttgatc atttcagtta cggcaggggg agagacttgc atgcgagccg caatttcctt
     9001 gttggtaatc ttatgcaggt ctatgccaat ttcataaata cattttagat agtcttcttt
     9061 gtttggggtc attcgtttcc tcttgtctaa tatctttatc tagtatatca aaaaaagcta
     9121 gatttctcta gctgtgactt tttatgttat acttattgca agagaaaaaa cgaggagatg
     9181 gatatgacga aaatagctct tctttcagat attcatggaa ataccaccgc cttggaggct
     9241 gttttggcag atgctcggca gctaggagtg gatgaatact ggcttttggg agatattctc
     9301 atgccaggga caggacgtag aaggattttg gacttgttgg atcaactacc gattacggct
     9361 agagttttgg gaaactggga agacagtctt tggcatggtg tccgtaagga attggatagt
     9421 actcgcccca gtcaacgcta tctcttgcgc cagtgccagt atgttttaga ggaaatttcc
     9481 ctagaagaaa ttgaagtgct ccacaatcaa cctcttcaac ttcatcgtca gtttggggat
     9541 ttgacggtgg gaattagcca tcatctgcct gataagaact gggggcgaga gttgattcat
     9601 actggcaaac aagaggagtt tgaccgcttg gtgactcatc ctccctgtga tattgctgtt
     9661 tatggacata ttcaccagca gttgcttcgt tacgggactg gtgggcaatt gattgtcaat
     9721 ccgggttcga ttggccaacc tttctttttg gacgctcagc tacggaagga cttgcgggcc
     9781 cagtatatga ttttggagtt tgatgacaag ggcctggtag atatggactt ccgacgggta
     9841 gactacgatg tggcagctga attgcagctg gctaaagacc tgagacttcc ctattttgag
     9901 gtttattatg aaagtctggt caatgggatc caccatactc atcatcagga atttttgaga
     9961 gaattggctc agaaagaggg ctgcgatcgg gagttagacg actggttgaa aagtggtaac
    10021 gattgacatt acaactaaaa agggtataat aatagaaaaa gaagagtaga ggcagggtag
    10081 cctcgtaaaa aggagaagag gatgcaaatt ccaagtagat ttaccattgc gactcatatg
    10141 ctgataatca ttgccctcga ggggaaggaa agcaaggtga ccagtgattt tctggctgct
    10201 agtgtcgggg tcaatcctgt cattatcaga aagatcttgt cccagttgaa gaaggcagag
    10261 ctgatttcag tagcgcgtgg aacgggcgga acagagattg tcaaggacct taaggatatt
    10321 agtcttttag atgtttatca ggcggtcgaa tgtcttggta agacaggtca actcttcagt
    10381 ttccatgaca atccgaatcc caattgccct gtaggagctc atattcatga tgttttggat
    10441 caaaaattgg agagaattca gttgactatg gaggcagaac ttggtcagac cagtctagaa
    10501 aaagtcgtgg ccgatgcaga gagtcagatg aaggattaag agggataaat gccctctttt
    10561 tttctttgga taattatgat aaaatgtagc tattaaagga gtaagaaatg tatcttgtta
    10621 taggaattgt attagccttt atagtgtcat tttggaagga taatagaagt ttgtggaatc
    10681 cagtattatt tttattatcc ttcatttcaa gttattttta tctatcctac cttttttata
    10741 aaaatggata cgagaatgtt cagttagctt tttatgtatt tgcctttgtc ttacttcctt
    10801 ttttgctttt tctaagtggc atctttttaa tctataatgg ggtcattttg ttgaaacgag
    10861 agggacgttc caagccccat tatctctcaa tgctctttga tttttattga ctattagatt
    10921 ctatttaaaa agacttagaa tgcacatgaa agtatgtatt ctaggtcttt tgttttgtat
    10981 acatttaatt ttcaagagta ttatctattt ttccatgcat ggtaacgagt atcaatcaat
    11041 aactgggtaa ggcgacccaa ggtttctctt tcttttggag tgagggattg agcttggaca
    11101 aagagatgat gaatgtattc aagcgccttt aaggaagcca agtcattgat agagctaata
    11161 ccagcatcaa gaatagtgcc aaagaagagg tcgaagtaga gctgaagttc ttcgtcaggg
    11221 actgtggcca cccattcttt aaaggttgtg tctacttgcc ggctatcact gttggtctta
    11281 tccagttgaa cgaagtgctt gtcctcaatc tgccaactaa aggtatcgtg ctgggcgatg
    11341 ccacccaggg cagtactctg aacgatgatt tggtgagcag gaatttccag catcataccg
    11401 ataatggaac cttgtggaat gaatatcttg cttctatcca ttatcctttg ataacccgca
    11461 gtctgtgtca attcttgatg gagaccaggt gcatcaaatg tataaactgc tgtgatctga
    11521 ttttgcaaac tttgctcaat ttggctagca gcatagatag cgagatttcc tcccttggaa
    11581 tgcccagcca gaataacctt ttgcttagga tgatgggcaa aaaagttctt taaatagcga
    11641 agggcgtgct tttgagaagg aatttccttc atataggtca ggtggaaatc ttccttccag
    11701 ccaatgatac tgtcatctgt cccacgaaag acaatcagat aggtatcgag gctgacacga
    11761 taagtcatag ccgcaaattg cttttgcagt tcagggtcga tgtcgttgat aaaatgggag
    11821 agtttgcaat ttttgaagcg cttgtgttga gccaattcat ctaataattg aaggcgattt
    11881 ttgctagtaa gcatggtggg ctctcttgga acctgaggtg ctagatctaa aagacgttga
    11941 ggaagtgtgg agaccagatt atcaaaggag aggtaggtga tttctattaa ggttagaatg
    12001 tctaactcat tcaagggaag gtcgtaataa gaatcatatg cgacatcttt cagatagtca
    12061 aaaatattgg ccataagagc tcctttcttt ttatttatct tagcaaattt ccctcaaatt
    12121 agctagtagc atagcctgtt tgtgctggct aaaaacaggc tatttcaaat tcagtttcag
    12181 accatctagc atggaaaaat ctgttataat aatggaaaag gagaagcgca tgcacaagat
    12241 tttattaata gaagatgatc aggtcattcg tcaacagatt gggaaaatgc tctctgaatg
    12301 gggatttgaa gtggtcctgg tagaagactt tatggaagtt ttgagtctat ttgttcagtc
    12361 ggaacctcat ctggtcctca tggatattgg tttgcccttg tttaatggtt atcactggtg
    12421 tcaggaaatc cgcaagattt ccaaggtacc tatcatgttt ctttcttcga gagaccaggc
    12481 tatggatatt gtcatggcaa tcaatatggg ggcggatgac tttgtgacca agccttttga
    12541 ccagcaggtt cttttagcta aggttcaggg cttgttgcgt cgttcctatg agtttgggcg
    12601 tgatgagagt ttgctggaat atgctggtgt tatcctcaat accaaatcca tggatttaca
    12661 ttatcaaggg caagtcttga atttgaccaa gaatgaattc cagattttac gcgtgttatt
    12721 tgagcatgca ggcaacatcg tagcacgtga cgacctgatg cgggaacttt ggaacagtga
    12781 ctttttcatt gatgataata ccctctctgt caatgtggct cgtttgcgta aaaagttgga
    12841 ggagcaggga ttggtaggat ttatcgagac caagaaagga atagggtatg gattgaagca
    12901 tgcttgattg gaaacaattt tttctagcct atctgcgctc ccgtagtcgt ctttttatct
    12961 atctgctttc tttggcattt cttgtcttac tctttcagtt tttatttgcc agtctaggaa
    13021 tttacttcct ctactttttc ttcttgtgtt gctttgtaac catattattt ttcacttggg
    13081 acatattggt ggaaacgcag gtctatcgcc aggaacttct ctatggagag agggaagcca
    13141 agtctccttt ggaaatagct ttagcagaaa aattagaagc gcgtgagatg gaactctatc
    13201 agcagaggtc aaaagcagaa agaaaactga cggatttgct ggattactat accttgtggg
    13261 tccatcagat aaagaccccc attgcagcca gtcaactctt agttgcagaa gtggtcgacc
    13321 gccaactgaa gcagcagcta gaacaggaaa ttttcaaaat cgactcctat accaacctag
    13381 ttttacagta cctgcgttta gaaagtttcc atgatgattt ggtcttaaag caggttcaaa
    13441 ttgaggactt ggtcaaggaa ataattcgta aatatgctct tttctttatt caaaaaggct
    13501 taaatgtcaa tctacatgac cttgataaag aaatcgtgac ggataaaaag tggctgctag
    13561 tggttattga gcaaatcatc tcaaacagtc tcaagtacac caaggaaggt ggtctggaga
    13621 tttatatgga tgaccaagag ctttgtatca aagatacggg aatcgggata aaaaacagtg
    13681 atgtcctccg agtatttgaa cgtggctttt caggatacaa tggccgtttg acccagcagt
    13741 cctctggact tggcctttat ctatctaaga aaatttctga agaactgggg caccagattc
    13801 gtatcgagtc tgaggtcgga aaaggaacga cagtgcggat tcagtttgct caagtgaact
    13861 tagtccttga gtaaaaatag atgagaaaaa gcctccagat tggaggcttt tatgctagac
    13921 tcttatttct caacgcgtga tttgttggcg atatcagcta ggatagcttg aacaaaatta
    13981 tcaactgaga cagtttgtgt ttctttttgg ccgtagcgac gaacgttgac tgtttcgtct
    14041 tccatttctt tgtctccaac aattaattgg taaggaatct tgctagtttg tgaagcacgg
    14101 atcttgaact gcattttttc attgcgctca tctacgtctg cacggacacc gcggtcacgg
    14161 agtttcttgg ctacttccca agcgtagtcc acgtgttttt cgttagatac tgggataagg
    14221 gttacttggt gtggtgccag ccatgttggg aaggcaccct tgtagttctc aatcaagata
    14281 gctgtgaagc gttccatagt tgagataacc ccacggtgga tcatgactgg acggtgatct
    14341 tcgccatcag ctccgatgta tttgaggtcg aagcgttctg gcaacaagaa atcaagttgg
    14401 atagtagaaa gggtttcttc ttttccaagg gcagttttaa tctggatatc caattttggt
    14461 ccgtagaagg ctgcttcacc ttcggcttca aagtagtcca cgcccatttc atcaagggct
    14521 gcacgaagca tggtttgggc attttcccac atctcatcgt tatcaaagta cttatgagta
    14581 tcttgagggt cacgaagaga gaggcggaag cggtagtcag tcaagttgaa gtcttcataa
    14641 acatcgataa tcaactgaag ggcacgttgg aattcttctt ggatttgttc tggagtaacg
    14701 aataggtgac cgtcgttgag tgacatttca cgtacacgtt gaaggccagt gagggcacca
    14761 gatttttcgt aacggtgcat cataccgatt tcagcgatac ggattggcaa ttcacggtaa
    14821 gagtgaacat ggtgtttgaa aacttggatg tggtgcggac agttcattgg acgaaggaca
    14881 aattcttccc cgtcacccat gtccatggtt gggaacatgt cttcttggta atgatcccag
    14941 tggccagaag tcttgtaaag ctcaacagaa gcaagtggtg gagtgtagac gtgttggtag
    15001 ccagaagcca actctttgtt tacgatgtag cgttccaatt cacgacggat agtcgcacca
    15061 tttggcaacc agaatggcaa accttgtccc acttcttgtg aaatcataaa gaggtcaagc
    15121 tctttaccaa gtttacggtg gtcacgttcc ttagcttctt cacgcatttg aaggtagttt
    15181 ttcaagtctt tcttgtcaaa ccaagctgta ccgtagatac gttgcatcat agcgttgtcg
    15241 ctgtttccac gccagtacgc accagctaca tggagaaggt ggaagatttg gatacgacct
    15301 gttgatggaa catgaggtcc acggcagagg tctacatatt caccctgacg atagatagtc
    15361 aaaccgcctt cgtcttctga gtgttcttca atcaattcca acttgtaagg gtcatttttg
    15421 aagatttcac gtgcctcgtc tttagtcact tcttcacgaa tagatgggaa gttttctttg
    15481 acgatttttt gcatttcttc ttcgatacga ggaaggtctt cgttagagat ttgaccagct
    15541 gtgttgtcag tatcgtagta gaaaccatct tcgatggctg gaccaactcc caagtgaatg
    15601 tctgggaaaa gacgacgagc tgcttgggcg aacaagtgag ctgctgagtg acgcaagatt
    15661 ggaagggcat cttcgtgatc aggtgtcaca atttcgatgc ttccatcttc agtgatagcg
    15721 cgagtagtgt cgatgagttt gccgttgaat ttaccagcca aggctttttt agctagggaa
    15781 ttgctgatag attgggcaat ttcaaaagtt gttacgccag attcgaattc acgaacagcg
    15841 ccatctggga aagtaatgtt aatcatgatg ttctcctttt ttattatttc atagtttcta
    15901 agcattgatt ttttatcagc agactacttc caagtcctaa cgttcaattg cttttcttta
    15961 tcagagagga agttttccat ttccgtcaga atgtctatat ccaaacgttg agaaagttct
    16021 aatagtcacc agatattttc tgaaagtttt tgttccagtg tgtagggtgt ttcatcatag
    16081 tagcgtcctt gttttgtcat caccagtcgt tggaaatttc caatatcatt agataaagcc
    16141 aagaggtctt cttctaccgt ccacttggaa tcatgatgct taacttccag ttcgttataa
    16201 gcttgtcgga ttgcccaact gcgctccact aattcttgca actccataat tagtatccta
    16261 ataaaaaaat gcgacatcct cgaaaggacg tcgcaacgtg gttccacctt catttatgta
    16321 cctcaaaaga gagggacacc tctgattggc tctaacgtgg ccaccgtttt atgttttcat
    16381 aaaaaactca agagtagtat cagtttagac ttcctatgcg tttccagcaa ccacgcactc
    16441 tctagtagaa agggactaag tgacttgtct ctatggatta ttatagcatg attgtgagat
    16501 ttttcaagga ttttgtgaaa gttttttgtt tttcattttt tgtagaatat gcttgatgct
    16561 aataccagaa tccatgccca ccagagcaac tgtttcatag attagcaagt aaattagtat
    16621 aaactcacca aaggttatga tagtaaagta aaagagcagt ccgttaccaa ctaaccatca
    16681 aagtgagaaa cggaaacggt agctatagag taacgataca atcaagatta ccagaggtgt
    16741 aaagaagaga agattgttta tatagtgtaa gggggtcaga aacaactagc aagaacaact
    16801 cataaagtgg ccaataaaag aataatatga ccagatagta ggggagatgg gaatagaatc
    16861 tacgcatgat aatcacctcg ttttaaaaaa acagacaact tagatctctt gctatgatta
    16921 ttatagcata tttaaaacaa gaaagtaaat ttgttgacaa agaagatttt ttttcactag
    16981 aatcatggtt agttttcttg gtagaaatat tttgataata attaagcaag actattgaca
    17041 ttgattatta agagtgtcac aattgctgta actaaaataa ttacaacaag agaggtataa
    17101 aaaatgaaac tagcagttat tgcagcaaat ggacaagcgg ggaaagcaat tgttgaagaa
    17161 gcagtcaaac gaggacatga agtaacggct attgttcgtt ctgaaaacaa aagtcaagct
    17221 gagtctatta ttaaaaaaga tttatttgag ttgacaaaag atgatcttac tggatttgat
    17281 gctgttattt cagcgtttgg tgcttataca ccagatacgc taccgctcca tagcaaatca
    17341 atcgaactat ttaatcagct tcttgctgga acacagacac gttttctagt tgtaggggga
    17401 gcaggaagcc tttatataga tgaaacaaaa acgacacgac ttttagatac gcctgatttt
    17461 cctgaagaat ttaagccact agcaaaagct caagcggatg aatttgatct tcttcgtaca
    17521 aaaaataatc tcaactggac ttttgtcagt cctgctgtag attttattcc cgatggtgaa
    17581 aaaacaggaa actacattct agcaggagaa atttttacta ctaatggaaa aggaataagc
    17641 cagattagtt atgcagatta tgctattgga ttggtagatg aacttgaaaa aggtcatcat
    17701 ataaaagaac gtatctcttt acttgaaaaa taataactat gagagtatta atgtttgaca
    17761 gaaaatttaa atcttggtaa aatagggatt gtcgtaaaga caaataactt cttcttggtt
    17821 acaggcatgc caacctgtca ctcggatgaa gccaaataaa aaggagaaac atcatggcaa
    17881 tctcaaaaga gaaaaaaaat gaaatcattg cacaatatgc acgtcacgaa ggtgatacag
    17941 gttcagtaga ggttcaagtt gctgtcctta cttgggaaat caaccacctt aacgaacaca
    18001 tcaaacaaca caaaaaagac cacgctactt accgtggatt gatgaaaaaa atcggtcgcc
    18061 gtcgtaactt gcttgcatac ttgcgtaaaa acgacgttaa ccgttaccgt gagttgatca
    18121 actctctagg acttcgtcgc taattcaaga tacaaaggcc gtcaaaagca caaagcaaaa
    18181 ataggaaaat tgacgaagaa acttcagttt ctaggagatt ttatcttttt tgccaagtgc
    18241 ttaggccgtg ttcaattgag catatcttga taatgaagct actctaaatt gggtagcttt
    18301 tttgcgttgt caccttacct cgatatactc aagtataatc tttggttacg gttcctagca
    18361 ctgtaaggta aaataaacca gcttttctcc cttcggggag attttttttg tttcactgaa
    18421 agtttcgtac gatcttcgcc tcgtcgccta gtctataaac gatttatcta gcaagaatca
    18481 tgatgctaag ggcgtcaaaa atccgtatga aaatagggaa atgacacagt gttcgatgag
    18541 cacaaggagt ttcatctttt tcactaggat tttagcccgg gctcaaatca gctctctgac
    18601 ttcagagggc ttttttattg aggttttatc ggtaacaatt ttggagacta caaaaacctc
    18661 aaacggtatc agctaattac accataaagg tgcgcagtta ctcggctttt caagccgagt
    18721 aactgtatgc aagcccctag aagagcccac tctttacaaa gtaaagtata gtatgttata
    18781 ctttacatgg aagtagtcac tgaattccag ttagaaatta ctttgtaact acgttttgag
    18841 gaggagtaaa atgctttcct acgttcgaca ttacccacta gcgatagcta aattaatgtg
    18901 tctgtgctct cctaaaatct gctgatttat tactgactaa tacaggagtt tttttttatg
    18961 ggacagacaa tcatatctgc tattggtgtt tatatttcca ccagtatcga ttatttaatt
    19021 attttaatta ttttatttgc acagctatca cagaataaac agaaatggca tatttatgcg
    19081 gggcaatatc taggcacagg cttacttgta ggggcgagtt tagttgctgc ttatgtcgtt
    19141 aatttcgtgc ctgaagaatg gatggttgga ttgcttggtt taatccctat ctatttaggg
    19201 attcgctttg caattgttgg agaagatgcg gaagaagaag aggaagaaat tattgaaaga
    19261 ttagaacaaa gcaaggcaaa tcaactgttt tggacagtta cattgctgac aattgcgtct
    19321 ggcggagata atttaggtat ctatatacct tattttgctt cgttagattg gtcacagacc
    19381 ctcgtggcct tgcttgtgtt tgtaatcggc ataattatct tttgcgagat tagtcgggtg
    19441 ttatcctcta ttccgttaat attcgagaca attgaaaaat acgagcgaat cattgtgccc
    19501 ttagtattca ttctacttgg actatacatc atgtatgaaa atggcacgat agagactttt
    19561 ctgatcgtgt agattttttt gtttcactag gattttagcc cgagctcaaa tcagctctct
    19621 gattttcaga gggctttttc attgagtata aatggacaat cttaaagtta ctctattgta
    19681 gagtggcttt tgatttgatt aggagattct tataaatata gtaaaatgaa acaaaaacag
    19741 gacaaatcga tcaggacagt caaatcgatt tctaaaaatg ttttaagaag tagaggtgta
    19801 ctattctagt ttcaatttga tatataaatg aaaaccttag ctgtttggcc aaggttcatt
    19861 tcttctatgc aagttcttct cttttcttat ctgggttcca gatgaagctt gcgataaagc
    19921 taaagatgat ggttaggata gcgaggataa tagcaaggat gagggcaggg atgcggataa
    19981 accaccagag tggatttggt tttttatcat tgtgccattg tttcgtttct tcgtccagac
    20041 gttggaattc tgtttgaata cgattggcga ctgtttcaat gccttcatca ttcattttct
    20101 tgatatctga gatatcgatt ggatttccaa agttcatatc gacacgttca cggctaatca
    20161 agcccttcaa agtcatggga ccggtgtagg taaccggcat gatacggacc ttggccattt
    20221 tggcaatcag tgctgcgccc cccttgacat cgtttgagtg gcggctacca cttggaaaca
    20281 tgatgagaga gcggtcactt tttttgagaa cgttgatagg atatttgatg gctgaggcgc
    20341 tgggattttc acggtcgatg ggaaaggcgc cacacatacg aatccaccaa ccaaagatac
    20401 ggttggtaaa gagttctttt tttgccataa agatgaactg ttttggcttg gtcgcaaagg
    20461 ccatataaac aggatcccac caggtacggt gaggcgcaac taaaatataa ttttcatctt
    20521 gattaggaat tttatcagta ttatgatagt gagcattgcc attgatggac cataagagca
    20581 atacaactaa tccacgcaaa taagtataaa acatgcgatc tccttcgatt gttttcttgt
    20641 tattattata ccttatcaaa ggagggctgg caaacttttc ccttgactag atgcatattt
    20701 gggatgaaat tagaattctg ttaaaaaaaa tgatataata gaatttatgg ataaaaataa
    20761 gattatggga ttaacccaaa gagaagtcaa ggaaagacag gctgagggtt tggtcaatga
    20821 ctttaccgca tcagccagta ccagcacttg gcaaatcgtt aaacgaaatg tctttaccct
    20881 ttttaacgct ttgaactttg ccattgcttt ggcccttgcc tttgtgcagg cttggagcaa
    20941 tctggtcttc tttgctgtta tctgctttaa cgctttttct gggattgtga ccgagctacg
    21001 agccaaacac atggtggaca agctcaatct catgaccaag gaaaaggtca aaaccatccg
    21061 tgatggtcag gaagttgctc ttaatcctga agaattagtg ctaggagatg tcattcgttt
    21121 gtctgcagga gagcagattc ctagtgatgc cttggttttg gaaggctttg cggaagtcaa
    21181 tgaagccatg ttaacgggag aaagtgattt ggtgcaaaag gaagttgacg gcttactttt
    21241 gtcaggaagt ttcctagcca gtgggtcagt tttatctcaa gttcaccatg tcggtgcaga
    21301 caactatgct gccaaactca tgcttgaggc taagaccgtt aaacccatca actcccgtat
    21361 catgaaatcg ctggacaagt tggctggttt tactgggaag attatcattc cctttggtct
    21421 ggctctcttg ctggaagcct tgcttttaaa aggcctgcct ctcaagtcat ctgttgtaaa
    21481 ttcgtcgaca gctcttttgg gaatgttgcc taagggaatt gcccttttga ccattacttc
    21541 gcttttgact gcagtgatta agctaggttt gaaaaaggtc ttggtgcagg agatgtactc
    21601 tgttgagacc ttggcgcgcg tggatatgct ctgtctggac aagacgggca ccatcaccca
    21661 aggaaagatg caggtggagg ctgttcttcc actgacggaa acttacggtg aagaggctat
    21721 tgccagcatc ttgactagct acatggccca tagtgaggat aaaaatccaa cagctcaagc
    21781 cattcgccag agattccaag gtcaagtagt ctatcctatg ctctctaatc ttcccttctc
    21841 cagcgaccgc aagtggggag ccatggagtt agaaggtttg ggaatagttt tcttaggggc
    21901 acctgagatg ttgctggatt ctgaggtccc agaagccaga gaggccttgg agagaggttc
    21961 acgtgtcttg gtcttagctc tcagtcagga gaaattagac catcacaaac cacagaaacc
    22021 atctgatatt caggctctag ccttgctgga aatcttggat cctattcgag agggagcggc
    22081 agagacgctg aactatctcc gttctcagga agtggggctc aagattatct ctggtgacaa
    22141 tccagttacg gtatccagca ttgcccagaa ggctggtttt gcggactatc acagctatgt
    22201 cgattgttca aaaatcacgg atgaggaatt gattgctctg gcggaggaga cagcgatttt
    22261 cggacgtgtt tcccctcatc aaaagaaact cattatccaa acgctgaaaa aagcgggtca
    22321 tacaacagct atgactgggg atggagttaa tgatatcctg gctcttcgtg aggcggattg
    22381 ttctatcgta atggcggagg gagacccggc aacccgtcag attgccaacc tggttctctt
    22441 gaactcagac tttaatgatg ttcctgagat tctttttgaa ggtcgtcgtg tggtcaataa
    22501 cattgcccac atcgctccga ttttcttgat aaagaccatc tactcattct tgctcgcagt
    22561 tatctgtatc gccagtgctt tactaggtcg gtctgaatgg atcttgattt tccccttcat
    22621 tccaatccag attaccatga ttgaccagtt tgtggaaggt ttcccaccat tcgttttgac
    22681 ttttgagcga aatatcaaac ctgtcgagcc aaacttcctc agaagatcca tgcttcgtgc
    22741 cctaccaagt gccctcatgg tcgtgtttag cgttcttttt gtgaaaatct ttggaacgag
    22801 tcaaggttgg tctgagttag aaatctcaac tctcctctat tatctcttgg ggtcaattgg
    22861 tttcttatcc gtatttagag cctgcatgcc attcactcta tggcgagtcc tattgattgt
    22921 ctggtcagta ggaggcttcc tagccacagc tctcttccca agaattcaaa aactgcttga
    22981 aattgcaacc ttaacagaac aaacgttgcc tgtttatggt gtcatgatgt tggtctttac
    23041 agtgattttc atcctgacta gtcgctatca agctagaaaa taaagaaaga ctgcaatctg
    23101 tggattgcag tttttttagg tacaagattg ccagccgaaa tgtggtataa taagaggtaa
    23161 tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt agcttccttt
    23221 gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct gcctatcgag
    23281 ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga aggtccctat
    23341 cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca agtccgttta
    23401 gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac cattgctggc
    23461 atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt agctaatgta
    23521 gagcgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg gctacaggat
    23581 catggattcc agattgtagc agaaagcatc ttagaagaag ctggcaagtt ttacgagatt
    23641 ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt tggtcccttc
    23701 ttgtccaaag aagtcagtcc agtatttgtc caaaaatggc aaaaagaagc tgagaagcta
    23761 gagttcgccc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt tctagtagat
    23821 aagattcaag ctatcaagga ggtgccccat gttagcaagt gaagtgatcc aagcttatga
    23881 agccttttgc cctcaggaat tttctatgga gggagacagc cgtggtctgc aaattggcac
    23941 tttagacaag ggtatccaaa gggtcatggt tgccctcgat attcgtgaag agacagtggc
    24001 agaggccatt gaaaagggtg tggacttgat tatcgtcaag cacgcgccta tctttcgtcc
    24061 tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc tgattaagca
    24121 tgatatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg ggctcaatga
    24181 ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa caggtccaga
    24241 acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat tggcccaaca
    24301 ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag agaatgattt
    24361 gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt tctataagga
    24421 tgctttggca aagggggcag atgtctatat cactggcgac atctactacc atactgctca
    24481 ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg aagtgctttt
    24541 tgtggaaaaa atcgcagcac tccttactca atggaaagag aaaaaaggct gggaactaga
    24601 gattttacct agtcaagcat cgaccaatcc tttccgtcat atctagttag aaagtgaaga
    24661 caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct gcctactacc
    24721 tctcgagaga aagtgactta gaggtgaccg tttttgacca tgagcaaggt caagccacca
    24781 aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa gcctggtaca
    24841 agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta gagaaatcag
    24901 gacaagaaat cgacttttac cagcgttcgg gagtctttct tttgaaaaag gatgaatcca
    24961 agttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc ttgatagggc
    25021 aattagccat tctggaccaa gcttcagcta atgaattatt ccctggtttg cagggatttg
    25081 accgcctgct ctatgcttct ggtggagcga gagtagatgg ccaactcttg gtaactcgtt
    25141 tgctagaagc cagtcatgtc aagctggtca aagaaaaagt gactctgaca ccgttagcat
    25201 caggctacca gattggcgaa gaggtgtttg atcaggttat tttggcaacg ggagcttggt
    25261 tgggggacat gttagagcct ttaggttatg aagtggatgt ccgtcctcaa aaaggacaac
    25321 tacgagatta tcagcttgcc caagacatgg aagattaccc tgttgtcatg ccagaagggg
    25381 agtgggattt gattcccttt gcaggtggga aattatcctt gggcgctacc catgaaaatg
    25441 acatgggatt tgatttgacg gtagatgaac ccttgctcca acaaatggag gaggccacct
    25501 tgactcacta tctgattttg gctgaagcta cttcaaaatc tgagcgtgtt ggaatccgtg
    25561 cctacaccag tgatttctct cctttctttg ggcaggtgcc tgacttaact ggtgtctatg
    25621 cagccagtgg actaggttca tcaggcctca caactggtcc tattattggt taccatttag
    25681 ctcaactgat ccaagacaag gagttgacct tggaccctga aaattaccca attgaaaact
    25741 atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa actaggaaac
    25801 tagccgcggg ttactcaaag cactgctttg aggttgtgga tagaactgac gaagtcagta
    25861 accataccca tggcaaggcg acgttgacgt ggtttgaatt ggattttcga agagtataag
    25921 cattttactg aaattttatc aggtagttta ggatggcaaa tgacattccc tgtcaaaaat
    25981 ggtaaaataa gaaaaaataa tctgagaatc gaggaaaaaa gatgcaagaa aagattttgg
    26041 taactggtgg tgccggtttt attggaaccc acactgttat tgaattgatc caagcaggcc
    26101 atcaagttgt tgtggtggat aatcttgtca atagcaatcg taagagttta gaagttgttg
    26161 aaagaatcac aggagttgag attcctttct atgaggcaga tattcgtgac acagataccc
    26221 tcagagatat tttcaagcag gaagaaccga ctggtgtcat tcactttgct ggtttgaagg
    26281 ctgttggcga atcaacacgt atccctcttg cctactatga caacaatatc gctggaactg
    26341 tcagcctttt gaaagtcatg gaagaaaaca actgtaaaaa catcatcttc agttcttctg
    26401 cgacagttta cggggatccg cacacagtgc ccatcttgga agatttccca ctttcagtga
    26461 ccaacccata cggtcgtact aagctcatgc tagaggaaat tttgactgat atttacaaag
    26521 cagactcaga atggaatgtt gtcttgcttc gttactttaa cccaatcgga gcccatgaga
    26581 gtggtgattt gggagaaaat ccaaacggta ttccaaacaa tctcttgcca tatgtgactc
    26641 aagtagccgt tggaaaatta gagcaagtgc aagtgtttgg agacgattac gatacggaag
    26701 atggaacagg tgttcgtgac tatatccacg ttgtcgattt ggctaagggt cacgttgcag
    26761 ctttgaaaaa aatccaaaaa ggttcaggac taaacgttta taaccttgga actggtaaag
    26821 gttactcagt tcttgaaatt atccaaaaca tggaaaaagc ggtgggacgt cctattcctt
    26881 accgcatcgt agaacgtcgc ccaggtgata tcgctgcctg ctactcagat ccagcaaaag
    26941 ctaaagcaga actcggttgg gaagcagaac tcgacatcac ccaaatgtgt gaagacgcat
    27001 ggcgttggca aagcaagcat ccaaatggat ttgaagacta agatgatgat ttcaatcatc
    27061 gtcccttgtt taaacgaaga ggaagtactt cctctttttt atcaggctct ggaagcttta
    27121 cttccagatt tggaaacaga aatcgagtat gtctttgtcg atgatggatc gagtgatggg
    27181 accttggaac tcttaaaggc ctatcgggag caaaatccgg cagcccatta tatttctttc
    27241 tcgcgaaatt ttggcaaaga agcagccctt tatgcaggct tgcaatatgc gacaggagat
    27301 ttggtggtgg tgatggatgc agacctccaa gatcctccta gtatgttgtt tgagatgaaa
    27361 aatgtactag acaaaaatgt agacttggac tgcgttggga cacggagaac tagtcgggag
    27421 ggagaaccct tctttcgcag tttctgtgct gttctctttt atcgcctcat gcaaaaaatc
    27481 agcccagtag ctctgccgtc gggtgtccgt gattttcgta tgatgagaag gtctgtggtc
    27541 gatgccattt taagcttgac tgagtccaat cgtttttcaa aggggctctt cgcctgggtg
    27601 ggtttcaaaa cctactattt ggactatcca aatgtcgaaa ggcaggctgg caagaccagt
    27661 tggagtttta ggcaactctt tttctactcc attgaaggga ttattaattt ttcagatttt
    27721 cccttgatta tagcctttgt agctggtctc ctatcttgtt ttctttctct gctgatgacc
    27781 ttttttgttg tggttcggac cctcattttg ggcaatccga catctggttg gacctctctg
    27841 atggctgtta ttctctttct tggaggcatt caactcttga ccattgggat tctcggaaag
    27901 tatatcagta agatttattt agagactaaa aaaagaccac tttatcttat caaagaaaaa
    27961 agtgaccttc ctgattttac agaaaaaaat aaagtgaaaa gactataatt ttacatggaa
    28021 atatgctaaa ctagagggag tggattatcg ccattgattt aggcttcctg gtttccaatc
    28081 agggcgcgag ttgggcaatt tttaacagcc tctaagacat cctgactagg agaaatttct
    28141 ttttccagtt ggtcagggtc atcgtaaaaa cgcacgattc cattatcgtg gtaatcaaat
    28201 aaatcagaat aagtttggca aagcccacag gcgatacatc gttcaggtat aagtgtgatt
    28261 ttcatgttca tatttatatt gtaataagaa aagtaaaaaa aaacaaggag taaggtatgg
    28321 caaaagaacc gtggcaagaa gatatctatg atcaagaaga atcaagagca gagcgtcggc
    28381 atcgaaacca cggaggggct gataggatgg ctaatcgtat tttgacgatc ctagctagta
    28441 ttttctttgt aattgtggtg gtgatggtca tcgttctcat ctatctatca tcggggggga
    28501 gtaatcgcac agcagcctta aaagactttc atgattctga tgcaagtgta gtacaaatct
    28561 catcttcaag cagttctcag cctgagcaga gttcagagct agaatctact tctagtagtt
    28621 cagaagaagc tgctaatcct gaaggaacga ttaaagttct cgcaggagaa ggggaagcag
    28681 ctattgccgc tcgtgcagga atctccattg ctcagttaga ggccttgaat cctgggcaca
    28741 tggctacagg atcttggttt gctaatccag gtgatgttat aaaaataaaa taggagccga
    28801 tatgaaaaca attcaaattg ctattgatgg tcctgcttcc agcggtaaga gtacggtcgc
    28861 aaagattatt gctaaggatt ttggattcac ctaccttgat acaggagcta tgtatcgtgc
    28921 agcgacctat atggctctta agaaccaatt aggagttgaa gaagtcgaag cccttctagc
    28981 cttgttggac cagcatccaa tcagctttgg acgttcagaa actggagacc agcttgtttt
    29041 tgtaggagat gtggatatta cccatcctat ccgtgaaaat gaagtgacca atcatgtttc
    29101 tgctattgca gcaattcctg aagtgcgtga gaaactggtt tctctccaac aagaaattgc
    29161 ccagcaaggc gggattgtca tggatggtcg cgatattgga actgttgtat tgccacaagc
    29221 agaattgaaa attttcctag tagcttctgt tgatgagaga gcagagcgtc gttacaagga
    29281 aaatattgcc aagggaattg aaacagacct tgaaacccta aaaaaggaaa ttgctgcgcg
    29341 tgactacaag gatagtcatc gtgagacttc tcctctcaaa caagcagagg atgctgtcta
    29401 ccttgataca actggtttga acattcaaga agtagttgaa aaaatcaaag cagaagctga
    29461 aaaaagaatg taaatgtaga aaagccgata gattgatatc ggtttttttc taaatttgtc
    29521 aaaatgctga ttttagggta taatagttgc tgttcgagaa attttgattt tcaaagaata
    29581 gtgaatgatg ataaggagaa accatgaaca atttaccaaa ttgtccaaaa tgtaactcag
    29641 agtatgtcta cgaagacggt gccctactgg tttgcccaga gtgtgctcat gagtggaatc
    29701 ctgctgaagt tgcagaagta gaagaaggtc ttgtcgctat cgatgccaac ggaaataaat
    29761 tgactgatgg tgatacagta actctcatca aggacttgaa agtaaaaggt gcgccaaaag
    29821 atttgaaaca agggacgcgc gtgaaaaata tccgcatcgt agaaggcgac cacaatatcg
    29881 actgtaagat tgatggtttt ggtgccatga aacttaaatc agagtttgtg aggaagattt
    29941 aagcatgtca aaacactata aacttgtatt ttatagccgt atcttcttgt ttctagcggc
    30001 ttttacggga gtttatcttg aaattactag gcatggtggt tttgggatgc ttctctatta
    30061 cacggttctg tccaacctct tggtaactat ttttaccctt tatcttctaa aggttatgag
    30121 ccgtgtaggt gaaaattggc aaaggccaag tctcttgcgc ttaaaaggtg gggtcaccat
    30181 gagtatcatg attacctgtg tgatttacca tttcctcttg gcgcccattg cgactaattt
    30241 ctatacccta gaaaatttcc tttgccacta tatcgttccc atctggtttt tagcggatac
    30301 cctctttttt gacaaacagg gtcaatacaa gatttgggat ccagcagtgt ggaccatttt
    30361 accctttctg tatatgatgt ttgctctttt taatggcttg gttctaaaac tcaatattcc
    30421 aaatgccaag gataatcctt tcccttactt ctttttgaat gtgaacaagg gttggaatgt
    30481 tgtgtttaag tggtgtctga ttatctttgt tgcctatatg gtagcaggat ttattttcta
    30541 ctttatcaag caaatcaaga gaaagtcatc ctaatactct tcgaaaatct cttcaaacca
    30601 cgtcagtttc catctgcaac ctcaaaacac tgttttgaac aacctgcggc tagcttccta
    30661 gtttgctctt tgattttcat tgagtataag atacaaggga ttcattttcg aatttaagaa
    30721 ggagtctaca aaccaatgag gcttcttctt ttcttgctta tttgataact atcaaaaaag
    30781 tagaagatta agagaaatat agaaagagat ttcatgaaac aatttttaga acgggccagc
    30841 attttggctc tctccctcgt tttgattacc tccttttcca tttcgagtgc tctaccagcc
    30901 atgtttgact attatcaggg ttattctaag gaacaaattg agctcttggt gagcttgcct
    30961 tcctttggaa tcatgatgat gttactgcta aatggtttct tagaaaaaat atttcctgag
    31021 cgcttacaga ttagtttggg cttgctgatt ttatcattga gcggtacagc tcccttctgg
    31081 taccaagcct atccctttgt ctttggaaca cggcttctct ttggtttggg tcttgggatg
    31141 atcaatgcca aggccatttc tattatcagt gaacgctacc aaggaaaaag gcgaattcag
    31201 atgttagggc tacgcgcttc tgcagaggtc gttggagctt ctctcattac cttggccgtc
    31261 ggtcagttgt tggcctttgg ttggacagct atctttctag cctatagtgc tggatttttg
    31321 gtgctgcccc tttatctgct ctttgtccct tatggaaaat caaagaaaga agtcaagaaa
    31381 agagcgaagg aagcaagtcg tttaactcga gaaatgaaag gcttgatttt taccttagct
    31441 atcgaagcgg cagttgtagt ttgtaccaat acagctatta ccatccgtat tccaagtttg
    31501 atggtggaaa gaggattggg ggatgcccag ttatctagtt ttgttcttag tatcatgcag
    31561 ttgatcggga ttgtggctgg ggtgagtttt tctttcttga tttctatctt taaagagaaa
    31621 ctgctcctct ggtctggtat tacctttggc ttggggcaaa ttgtgattgc cttgtcttca
    31681 tccttgtggg tggtagtagc aggaagtgtt ctggctggat ttgcctatag tgtagtcttg
    31741 acgacggtct ttcaacttgt ctctgaacga attccagcta aactcctcaa tcaagcaact
    31801 tcatttgctg tattaggctg tagtttcgga gcctttacga ccccatttgt tctaggtgca
    31861 attggcttac taactcacaa tgggatgttg gtctttagta tcttaggagg ttggttgatt
    31921 gtaatctcta tctttgtcat gtacctactt cagaagagag cttaggattg attcctaagt
    31981 ttttcttttg atactcaatg aaaatcaaag agcatagttg attgagtttg gaatagtacg
    32041 ctgtagttgc taaaatattt ctagaaatta atttgacttt tctaatagag ttattcatat
    32101 cttatttcaa tctactatag aaagctagcc gcaggttgct caaaatagtg ttttgaggtt
    32161 gcagatggaa actgacgtgg tttgaagaga ttttcgaaga gtatgagatt tttataccca
    32221 tacaatattt gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggttatg
    32281 acgagatata aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag
    32341 tctcatgcgc gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg
    32401 caaaccatta ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag
    32461 gtcattcatt ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat
    32521 acccagtctc ctgaagatat tgatgtgatt tcgattgagc ttgtggcaga tgattttcat
    32581 tgccgttatg ccaaacatag caagacctat gagtttattt tggatagagg acgtcccaaa
    32641 aatcctatgc gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg
    32701 cagattgcaa tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg
    32761 actagtgtag aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca
    32821 ggacaatttt tgacctttac cttttcagga aatggtttct tgtataaaca gattcgcaat
    32881 atggtgggga ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc
    32941 atcttggaga agaaggacag gaaacttgca ggtcccactg cagcaccaaa tggtttgtat
    33001 ttaaaggaga ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt
    33061 tagtggtggt ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt
    33121 tgtagcagtg acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga
    33181 tgataccatt tttcaacatg aattagatag cttgcgtgat gtggaatttg ggggaattaa
    33241 gattggtctt ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg
    33301 cccaggagta cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc
    33361 tgtcagtgag ctctgccaag agttgattcg ctttttccct tatgtcagtg tgattacgcc
    33421 taatctccca gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa
    33481 aactgcagcg cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa
    33541 tcgtcttagt caggacaagg ctgtggatgt cttttatgat ggacagacct ttactatcct
    33601 agaaaatcca gttatccaag gccaaaatgc tggtgcaggt tgtacctttg cctctagcat
    33661 tgccagtcac ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt
    33721 cgtttatcgt gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa
    33781 aaacaactaa aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt
    33841 tcttaaaaat cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg
    33901 cggcctttta ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttga
    33961 ttgacctctt atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc
    34021 agggattttt cgcaggattt aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta
    34081 ttgccatggt aggaggctac gccttgggtt ctactttgat gaatggctgg gcagcagccc
    34141 tcccagaaat tctaccaaat ttcatgcaaa atatggtagg gatgattgta ggatttattc
    34201 ttagtcaaag tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca
    34261 gtttccatct gcaacctcaa aacactgttt tgagcagcct gcggctagct ttctagtctg
    34321 attttcattg agcataaaga ggttgagtaa aaagtctttc aaataagaaa aacgcatagt
    34381 ataggatgtt gaaatgatga actgcccccc aaaagttaga cagaaaaaat ctaacttttg
    34441 gggtgttttt attatgaaac taacttatga tgataaagtt cagatctatg aacttagaaa
    34501 acaaggatat agcttagaga agctttcaaa taaatttggg ataaacaatt ctaatcttag
    34561 gtacatgatt aaattgattg atcgttacgg aatagagttc gtcaaaaaag gaaaaaatcg
    34621 ttactattct cctgtttaaa acaagaaatg att
//
LOCUS       DBERCA010000015        34051 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.015, whole genome shotgun sequence.
ACCESSION   DBERCA010000015 DBERCA010000000
VERSION     DBERCA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.015"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(71..751)
                     /locus_tag="ACL719_000862"
                     /pseudo
     CDS             complement(71..751)
                     /locus_tag="ACL719_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(839..1360)
                     /locus_tag="ACL719_000863"
                     /pseudo
     CDS             complement(839..1360)
                     /locus_tag="ACL719_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1380..1475)
                     /locus_tag="ACL719_000864"
                     /pseudo
     CDS             complement(1380..1475)
                     /locus_tag="ACL719_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1732..2055)
                     /gene="tnpB"
                     /locus_tag="ACL719_000865"
     CDS             complement(1732..2055)
                     /gene="tnpB"
                     /locus_tag="ACL719_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0698863.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWLSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(2036..2221)
                     /locus_tag="ACL719_000866"
     CDS             complement(2036..2221)
                     /locus_tag="ACL719_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698864.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            complement(2525..4087)
                     /gene="guaA"
                     /locus_tag="ACL719_000867"
     CDS             complement(2525..4087)
                     /gene="guaA"
                     /locus_tag="ACL719_000867"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGS0698865.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4229..4927
                     /locus_tag="ACL719_000868"
     CDS             4229..4927
                     /locus_tag="ACL719_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0698866.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4970..5866)
                     /locus_tag="ACL719_000869"
     CDS             complement(4970..5866)
                     /locus_tag="ACL719_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0698867.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5875..6810)
                     /locus_tag="ACL719_000870"
     CDS             complement(5875..6810)
                     /locus_tag="ACL719_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGS0698868.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTASELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6807..7532)
                     /locus_tag="ACL719_000871"
     CDS             complement(6807..7532)
                     /locus_tag="ACL719_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGS0698869.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     SYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7616..8251)
                     /locus_tag="ACL719_000872"
     CDS             complement(7616..8251)
                     /locus_tag="ACL719_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGS0698870.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(8253..9080)
                     /locus_tag="ACL719_000873"
     CDS             complement(8253..9080)
                     /locus_tag="ACL719_000873"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0698871.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9102..9438)
                     /locus_tag="ACL719_000874"
                     /pseudo
     CDS             complement(9102..9438)
                     /locus_tag="ACL719_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(9439..10155)
                     /locus_tag="ACL719_000875"
     CDS             complement(9439..10155)
                     /locus_tag="ACL719_000875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGS0698872.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRVQAPLVIKEVNGIKVALLAYSYGFNGIEQYISKEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSEMVEKDGDKKLIIY"
     gene            10668..11279
                     /gene="def"
                     /locus_tag="ACL719_000876"
     CDS             10668..11279
                     /gene="def"
                     /locus_tag="ACL719_000876"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS0698873.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11324..12052
                     /gene="rlmB"
                     /locus_tag="ACL719_000877"
     CDS             11324..12052
                     /gene="rlmB"
                     /locus_tag="ACL719_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGS0698874.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12091..13002)
                     /gene="trxB"
                     /locus_tag="ACL719_000878"
     CDS             complement(12091..13002)
                     /gene="trxB"
                     /locus_tag="ACL719_000878"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGS0698875.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13068..13292)
                     /locus_tag="ACL719_000879"
     CDS             complement(13068..13292)
                     /locus_tag="ACL719_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGS0698876.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13370..14113)
                     /locus_tag="ACL719_000880"
     CDS             complement(13370..14113)
                     /locus_tag="ACL719_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0698877.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14113..14913)
                     /locus_tag="ACL719_000881"
     CDS             complement(14113..14913)
                     /locus_tag="ACL719_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0698878.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15071..15427)
                     /locus_tag="ACL719_000882"
     CDS             complement(15071..15427)
                     /locus_tag="ACL719_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS0698879.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15440..15952)
                     /locus_tag="ACL719_000883"
     CDS             complement(15440..15952)
                     /locus_tag="ACL719_000883"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGS0698880.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNK"
     gene            complement(15952..16446)
                     /locus_tag="ACL719_000884"
     CDS             complement(15952..16446)
                     /locus_tag="ACL719_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS0698881.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16581..17027
                     /locus_tag="ACL719_000885"
     CDS             16581..17027
                     /locus_tag="ACL719_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGS0698882.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            17024..17671
                     /locus_tag="ACL719_000886"
     CDS             17024..17671
                     /locus_tag="ACL719_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGS0698883.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17692..18273)
                     /gene="pdxT"
                     /locus_tag="ACL719_000887"
     CDS             complement(17692..18273)
                     /gene="pdxT"
                     /locus_tag="ACL719_000887"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGS0698884.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREGILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18274..19149)
                     /gene="pdxS"
                     /locus_tag="ACL719_000888"
     CDS             complement(18274..19149)
                     /gene="pdxS"
                     /locus_tag="ACL719_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGS0698885.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19301..20680)
                     /gene="nox"
                     /locus_tag="ACL719_000889"
     CDS             complement(19301..20680)
                     /gene="nox"
                     /locus_tag="ACL719_000889"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGS0698886.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20974..21897
                     /locus_tag="ACL719_000890"
     CDS             20974..21897
                     /locus_tag="ACL719_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGS0698887.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21957..22562
                     /locus_tag="ACL719_000891"
     CDS             21957..22562
                     /locus_tag="ACL719_000891"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS0698888.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEETDGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22581..23825
                     /locus_tag="ACL719_000892"
     CDS             22581..23825
                     /locus_tag="ACL719_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGS0698889.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23942..24199)
                     /locus_tag="ACL719_000893"
     CDS             complement(23942..24199)
                     /locus_tag="ACL719_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGS0698890.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24241..26277)
                     /gene="glyS"
                     /locus_tag="ACL719_000894"
     CDS             complement(24241..26277)
                     /gene="glyS"
                     /locus_tag="ACL719_000894"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGS0698891.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26489..27406)
                     /gene="glyQ"
                     /locus_tag="ACL719_000895"
     CDS             complement(26489..27406)
                     /gene="glyQ"
                     /locus_tag="ACL719_000895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGS0698892.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(27601..28362)
                     /locus_tag="ACL719_000896"
     CDS             complement(27601..28362)
                     /locus_tag="ACL719_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS0698893.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDLNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28635..29477)
                     /locus_tag="ACL719_000897"
     CDS             complement(28635..29477)
                     /locus_tag="ACL719_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGS0698894.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSITISRIQANLDCFGIELSHKERETLKTIAVQLGAPRVD
                     DVDF"
     gene            complement(29591..30982)
                     /gene="pgdA"
                     /locus_tag="ACL719_000898"
     CDS             complement(29591..30982)
                     /gene="pgdA"
                     /locus_tag="ACL719_000898"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGS0698895.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31220..31588)
                     /locus_tag="ACL719_000899"
                     /pseudo
     CDS             complement(31220..31588)
                     /locus_tag="ACL719_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            31809..32786
                     /locus_tag="ACL719_000900"
     CDS             31809..32786
                     /locus_tag="ACL719_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS0698896.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            32783..33865
                     /locus_tag="ACL719_000901"
     CDS             32783..33865
                     /locus_tag="ACL719_000901"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0698897.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33929..>34051)
                     /locus_tag="ACL719_000902"
     CDS             complement(33929..>34051)
                     /locus_tag="ACL719_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS0698898.1"
                     /translation="AFRILEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
BASE COUNT        10068 a         7635 c         6121 g        10227 t
ORIGIN      
        1 ccaataacga agtatattga aaaatctcca gactagagaa ctcacggata gttcctaatc
       61 tggagatttc ttatttgcac ttttcttgta caactttagt ccacggtaaa taggcctcta
      121 aaacctcttt gtttacgaga gtttcctcgt ttggaagaca ttctagaaga taggatagat
      181 atttctcact atttagttga tgacgtttag ctgtttccaa taagctcata ataatagctc
      241 ttgctttagc tccttcaaaa actttgagaa aacaaccacc ctcgcttttt taaactgagc
      301 ttaggctaaa agagtccagt ggactctttt actccgtccc ataaccaatg atttaatggc
      361 gcgttcagct agattattgg aaaggaccag atgtccgtct tttaaaatgg tcttaaaggt
      421 ttcttcatac ttgaggctgt attcaattgc ccttcctagt tttgaacccg ataaaactga
      481 ctgacgacgg caccaagcaa agaagtcttc tattaggggt tggagctctt cttgacgttt
      541 ctgtagtcgt tcatcagctg gcaaagcctc ccagtctctt tccaaggaaa ataactgatc
      601 acaataggct aatcctttag ctcctaaaga tgatttatcc gcttgcttgg ggggcgcttc
      661 aaaaaacttc cttctcacat gtgcccaaca tccaacaagt tcagcttctg ccagttgact
      721 gacgataagc ttctagcggt tcgcagttta ccaagcttag cgtcgccttg ctcctaaacc
      781 ttggactgct atcgcatagg cagaactaga ggactaaagt cctaagttac tgccgcaaca
      841 tgtcacaatg cacatagcca gaataatctc ctaggaattc ttgtactact aaaccactcc
      901 gacgctgatc atggtggtac agcgtgattg cttgattttc agctttccca gacaaaaagg
      961 tccaatagta ggtcagctga ctatcactct ctagcaccct ataagaagtt tcatctgcat
     1021 gaagtaaggg cttgagtcaa tagtctcgtt ataaaggggc tttaggaata ggagctttca
     1081 cgattttatc actcggattt ttatcactgc atgcttggca tttgtaagcg tgttggatat
     1141 gatctatgcg ttttaattgc gcaggaataa agaccaattc ttgtccttga agactccctc
     1201 caatctcttt tagatctccc tgacaatcag ggcaaatcct ctcttctact tgatgatgaa
     1261 cttcttctga atcaaattgg gcaagaagag cttggcgttt ccctttagat ttcttacgtt
     1321 tgtaggtaat ttcttctgtt tcaactggga acatctccat cttcctcaga tggagactct
     1381 tcctcaaaaa ggttgagttg tccagagggg caaacacttt tctcagagga ttttccatag
     1441 agcttttgag tcagataagc cacttgttca cgcagaaggg caagttcatt ggtgagacta
     1501 tcaattgtag cactctgttg ttgaatgatt ttcaatcttc ttccatgagc ttccctctct
     1561 gttttctaac tttattatac taaaaagaaa gtcctcattt caatagaaat cacgactttc
     1621 tgatggattt atagtaaaat gaaataagaa caggacaaat cgatcaggac aatcaaatcg
     1681 atttctaaca atgttttaga agcagaggtg tactattcta gtttcaatct actatatttt
     1741 tggagtgata gaaaagccct tcataagcca atctacttgt tcaggtgcga gagctttgac
     1801 atccttttct gtacttagcc aagtcagttt tccgttctca aagcgtttat ataatatcca
     1861 aaatccttga ccatcccagt aaaggacttt aaagcggtct ttacgtccac cacaaaagag
     1921 aaagacttga tcggagaaag gatccaattc aaagtgggtt ttaaccagat aagccagtga
     1981 atcgattcct tgtctcatat ccgttttccc acatacgaga tagacctgcc ctaggctaga
     2041 tagatgaatt gtcatagagc aacaccttat ccaaaagctg ttctatcatt tcgagattga
     2101 gagattgaaa aaaacttact tcaagcttgc caatacgaat tttaagcaga atatcatttc
     2161 tctttttaga atcaaaagga cgagattgtg gaatctctac aggaacaata ggttgtggca
     2221 ttaaaatatc ctccaatagt tttactttac taatagtata caggaggagc tccatgaatg
     2281 atagatacct tgttatgacg cgcttactat attagaaagt tggctctcta attaaattaa
     2341 ttctcattat atatatcaaa agaccgcagt ttgaaaacta cggtctaatt ttttgtccaa
     2401 atgtaaacca cttggaacta ttattcaatt tattcttttt agcaaatgtt cccgttttaa
     2461 tcccagttac agtttttaat cctctgtaac cgttgatttt acaaagcttt taattttttt
     2521 gcgattattc ccactcaact gttgcaggtg gtttacttgt aatatcgtag acgatacggt
     2581 taacatgatc cacttcattt acgatacgta ctgagatttt ttgaagtact tcccatggaa
     2641 ttttggcaaa atcagcagtc ataccatcga tagaagtgat agcacggatt gcaatcgtgt
     2701 agtcatacgt acgaccgtca cccataacac cgactgaacg aacgcctgtg ttaacagtga
     2761 agtattgcca aatatcgcgg tcaagtccag ctttagcgat ttcttcacga agaatagcgt
     2821 ctgattcacg aacggtttca agtttctctt cagtgatttc acccatgaca cggatagcaa
     2881 gtcctggtcc tgggaatggt tggcgccata cgatatggtc tggcatacca agctctgtac
     2941 caagagcacg aacttcatcc ttgtaaagag tattgagtgg ttcaatcaat tcaaactgca
     3001 tatcttctgg aagaccaccc acgttgtggt gtgacttgat agtttgagct gtatccgtac
     3061 cagactcgat aacatctgta tataaagtac cttgagcaag gaatttcaca tctttgagct
     3121 tgcttgcttc gtcatcgaat acatagacaa actcgttacc gatgatttta cgtttttgtt
     3181 caggatcaga aacgccagca agtttgtcaa ggaaacgttt agcagcgtct gctttgacga
     3241 tattcaaacc aaacttacca ccgagcatgt ccataacttg atcagcttcg cctttacgaa
     3301 gaagaccgtg gtctacgaag atacagatca attgatcgcc aatcgctttt tggagaagaa
     3361 ccccaacgac agatgagtca acaccacctg atagaccaag aaggacacgt ttatcaccga
     3421 cggtttcacg aatttttttg atctgcatgt caatgaaatt atccattgac cagtcacctt
     3481 tagccttaca aatgttaagg gcaaagttac gaaggatatc atttccgtat acagaatgac
     3541 gaacttctgg gtggaattgg ataccgtaaa tgtgtttatc tgggttttcg atggctgcgt
     3601 atgggcagtc agctgatgta cctgtacgaa caaagtcagc aggaatctca gtaaccgcat
     3661 caccatggct catcaaaaca gtctgttcat caggtgttga ttcaaaaagc gctgatggtg
     3721 tgtgagttag ggttgattga ccgtattcac gatttccagc atcacctgca ggaacaactt
     3781 ttcctccaag tttatgggtc aataactgca taccataaca gattcccaaa attggaattc
     3841 cgagttcgaa gatttctggg tcaatatcaa atgaaccatc ttcatataca gaatttggac
     3901 cacctgatag aataattcct acaggattga cttcacgaac ttcagcagct gaaattttat
     3961 ggctttttag ttctgaaaaa acaccaatct cacggatacg gcgtgaaatc agctggttgt
     4021 actggctacc atagtccaat acgatgattt tttctacatc ttgcaaatca gttgaaatgt
     4081 tgctcatctt tttcctttct caaaagaaat atcttttccc aatattctat cacaaataag
     4141 ggcgaattac caactaaaca aggcgaagtt tgactttttc tagtttattg gggtacaata
     4201 gagaaaagat agaaatgaag tgaaaaatat gctacctgct tatatgaaaa tccatgatca
     4261 gataaaaaag gatattgacg agtaccgttg ggctattggt gagaggcttc ccagtgaaag
     4321 agatctagct gagcagtttg cggtcagtcg catgaccctc cgccaagccg tatctctatt
     4381 agtcgaagaa ggcgtcttag agcgccgtgt aggaagcggc acctttgttt ccagtactcg
     4441 agtacaagaa aagatgcgag ggacaaccag ttttactgaa attgtcaaat cccaaggtaa
     4501 agttccctct agccagctca tttcctacaa aaaaaccatt cccaatgagc aagaagttgc
     4561 caagctagga atttttccaa cggacaatat tatccgaatg gagcgggtcc gctatgccga
     4621 ccaagttccc ctagtttatg aagttgcttc tattcctgaa aaattcatta aggactttaa
     4681 aaaagaagaa atcaccagtc atttcttcca aaccttgcaa aaacatggct atcgtatcgg
     4741 caaatctcaa cagaccatct atgctcgcct tgctaaagaa aagattgccc actatttgga
     4801 agttgaaaaa ggacatgcta ttcttggatt gacccaggtt tcctacctag aagatggtac
     4861 tgcttttgaa tacgtaaaaa gtcaatatgt aggcgaacgc tttgaatttt atcttgaaaa
     4921 taattagatt accacgcaaa aactctctgt cacgaacaaa gagtcttttt tatttttcta
     4981 agaataaatc tttcaaagaa atcaaagtta cagcaaacaa tatcattgcc ataccaagaa
     5041 aatcaatggg ataaaattgt tcattcatta ttaagaaggc aaagaaaatc gccgatattg
     5101 gctcaattga agccaacaag cttgacttga ccggtcctat cagactggct cctttaagga
     5161 aagctgtata ggcaaagaca gtcccgataa ggataatgcc tgcaaacgca aggagaaaat
     5221 caagactagt cgggatatcg gcctgtagaa cccctgtaaa aggaagggcg accaaacctg
     5281 ctatgaccat tcccacacca atgaccaagc tgctccccca ctttttaatc aaggctatgg
     5341 gtaaaatgat atacagagca taagtcaagg cagaaaagag accccagaac agaccagcag
     5401 gtgtcatgga taactggtcc aactgcccat gtgttgcgat caggaaggtt cctccgatgg
     5461 cgaatatgat ggaaactatc tctcccagtg tcggtgccac cctatccttg atacagctat
     5521 aaattaaaat tccgacagga caaacatact gaagcaccgt cgctgttccc gcattggtct
     5581 cctgaatagc agacagatag gcgaattggt tgagaaaaag accaatcaga gcaaaaataa
     5641 gaagagacag caaactcttt ctatccttta aaaagaccag tattttatcc tttgcagtag
     5701 cataagccaa gagcatgaga attccaccag cgattaaaag acgcaagtta gtcaagacca
     5761 gagccgaaat tccgtgtgcc attaggtatt ggccactcgt tcctgacaac ccccaagcaa
     5821 taccagccac aactgttagt aaagtccctt ttaaactatt tgacatgtct ctccttactc
     5881 ttctttgcga atcaagttca tcacttgatt gcggtctaat atccattgag ctctctcgtc
     5941 cttttcatag gtacgattcg ataggaaaat agtggcttct tgcttctgac gattccacat
     6001 gataaaggta cctgtatagc ccgtatggtc tagccaatct ccttccaaat tccatgctaa
     6061 agaacgttcc ttgtcatcca aaggagaaaa attttgattt aagtctcttg caaaatcatc
     6121 tgctaaatag tgttctaaaa agatttgtaa atcctttata gtcgaaaata aaccagcact
     6181 cccagcatgt ctacccagga gacgagcctt gggatcatgc actatgcctg cctctacacc
     6241 tctaactgtt ggaacagcaa gctcaactgg cccaaacttg gtttccgtca ttccccaagg
     6301 tttccagact tgatccttta aaatcacatc caaatcttga ttaaaaattc tttccaaaat
     6361 aaagcccaac agcaaaaaat ggacatccga ataaagaaag gctggctgac ttcgtctgtt
     6421 gagatgaaac atcgcttcct ttaattcaga ggctgttaaa agatcacgat taggaataaa
     6481 aggatcaagg tctgttgcat gagtcaagag ctggcgaata gtgatgtctg gataatcact
     6541 ctcaggtaaa aaatctatta ccagtctatc aatatctaat tgacctattt cccacaagaa
     6601 ggtacaaact gtgccaaccc caacaacctt gctgacacta gctaggtcat aaactagtcc
     6661 tgcctcagtc tgcaagccat gctctggggc actctggcct aaatagaact ctgtccattg
     6721 attgtcctta aaatacgcaa aagaggctcc gggataaatt cctgcctcga tttgttcttc
     6781 tattttttta ataatcttgg tccacttcat acttcttcaa accacaattc aacattattt
     6841 ctatctttac caaggaagaa tttttcagac ttaggaataa aatattcagt agactcaaac
     6901 ttctggcgaa gacttgctat gtctaattca ttgaccaaga acttgagcat agataagtcc
     6961 caggtaaccg tattgtccac agtcaaatcc tgcccctgag ctggaataaa atcaagggat
     7021 gccccaattt cagatgattc caagaaactt tcgatatcag ttgggagatg taattccata
     7081 gaaatctcaa atttacttaa agaaattgat gccaaatctg tttgaaattc aggcttttct
     7141 cctacttcta ctagacttgc tatgtcatct tccgcatgaa tcaaaatcaa atcatcttct
     7201 ggtgagaaaa tttcaaaagc gtagctattt tgacctttat ataatcgatg aatcgaatct
     7261 gttttagata agattccttc aatttctaag ggattttcca ccttgacaat caatctagct
     7321 agtttttttc ttccctctac cttacgagta cgcatactgg gagcttcttc taaaaccagc
     7381 ttttcaagac ccgtttggtc acctagtgac agaaaggccg attcttctaa caaggccttc
     7441 attccaaggg tttcaatata aaatgtttca tttaattttc tattattagc ttttaaagta
     7501 ggaataatcc gtacaatctg atttacattc ataaattcct ccgtcacttt ttattttaaa
     7561 ggattttaga attttttaca agatattatg ctcaaattta acattattta gctatttagt
     7621 aaagatagtc tttcaaggct tttgacaaag cctgataacc agcaatactg aggtgcagtc
     7681 catcagttgt atattctttt ttgagttggc ctgcttggtc tgtcaaacaa tcaaatactg
     7741 gcacaaattc cacctgcata taggcagatg caagctcttg ataggcttga ttccagttct
     7801 gaattttttc attcgagcgg atatagactg cctgctggta ctcctctctc tcattgacag
     7861 gcaaaatgga aagcaattta atctctgtca atggataatc gcgagcaacg gattgaatga
     7921 tagcttcgag attattgaga gcctcattca caggaacatc ctttccgata tcatttgtcc
     7981 caatcagaag aaaaatttta tctactgctc caccatatag atgagcatca aggttctcta
     8041 gtaacagtcc tgtctgatag ccacgaattc ctcgattgac aatcgtcttt gaagtcccaa
     8101 atagctcctg tagaggataa tactcgacaa tggaatcccc aataaaaaga atgtttggtt
     8161 ctacaacaga aatgtgattt aggtgacgat acttagtttg aattttttct tgttccttta
     8221 ggagccaatt ttctaataac tgtactgcca cttcattctc ctttttctaa ccagttttcc
     8281 aaaacttgat aaactcctcc ttggctgttg gctggtgcta gagcagtcgc cacagctttg
     8341 gctttctcat cagcattttc catcgcatag gcaattccag ccatttcaag catttcaaca
     8401 tcattttcac tatcaccaaa agccatgatt tcttgggatt tcaagtccca gcgcttgagt
     8461 aattcctcca agccccatgc tttatgaatc ccagcttgga ggatatcaat gcaaccatag
     8521 ccactggata cagctcggac acggccatca aagagagcat tgatttcttc caaaaccgaa
     8581 ctcaaacgtt cctcaccaac aaccatgctc atcttgagca caccaccaaa gaggtcagat
     8641 gttaattcat ccacaaattg catccgttgg tagaattttt caatcatttc tggagtcata
     8701 aaactttcaa gatctgtaaa aatcgtacct tccttgacaa aatcaccctt catccccgtt
     8761 acaacaaact ggtcctgaca cgctcgaccc ttgaaatgag ccaaagcctt gttgacaatg
     8821 gcgtcatccc atgtctgagc ctgaatcaat tcattgtttt caaaaatacg agcgccatta
     8881 gcaacaacca gaaccactcg atccaccaag ggactcagta gttgtctcat gcggtgaatt
     8941 tcattgcccg tcgcaatgac aaaacgaatg cccctttgat ccaactgatc taaaatcttt
     9001 tccaatcgtg ggagatcaag ctgaccccta gcatccagca aggtcccatc catatctgtc
     9061 gcaattacct taatcatcat ttactttcct ctggattcaa gctaatacca cttcaatccc
     9121 acatgttcat tcatttcttt ataggctgta tcaattcgtt ccttagtcgc ttcatctaac
     9181 tggtcacgat gactgccacc cctctataaa atcttccaaa atataagttt ggtaatgata
     9241 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     9301 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     9361 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     9421 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccat
     9481 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc cccaatcgat
     9541 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     9601 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt cagccttcat
     9661 cttatcttcg tttaaatctg aaagataacg attatagtct tccttagaaa tatactgctc
     9721 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     9781 aatgaccagc ggagcctgaa cacgtggttc gtgcgtataa actccgattg gagtgattcc
     9841 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     9901 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     9961 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
    10021 atcaccaata gctaaatctg cttgcttgag ccaaggagtc acatactcaa aattttcatg
    10081 aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt ggtagagcaa
    10141 atccccgtgg gccataattc tggcagtttt ttcctcttcc tactcctgag acttttgctt
    10201 agtcccctct tgagaaatag tatctcgttt ttgactagtc aagggattcc ttggaagctt
    10261 tcaaacaaca agaccaagcc cattgataaa ccaactgcta gcaagagtat cgccacaaat
    10321 cccttattgc tccacttgcg ataactccta aaaagtttac caagcccttc ataaaacgaa
    10381 aagctaaacc accctgattt cgattttgtc ttctttgtat cttcgttctc cctactttct
    10441 tatgcaagcc ttttcttttt attatatcac agataagtat ttctttcaca attgaattga
    10501 acttcctatc tattttctat aaatcctaaa tgccataatg ctttcaattc ttgtcatttt
    10561 gtgatatcat gtagaggaaa tgaactaatc cacagtggct tattccaagt ataccacttg
    10621 ggctttggca gtagctaact gcactaaaca taatataagg agagaaaatg tctgcaatag
    10681 aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt gaagggaatc
    10741 ctactctacg cacggttgct gaggaagtca ctttccccct atctgaccag gaaatcatcc
    10801 taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg gctgaaaaaa
    10861 tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca aaacgcatta
    10921 tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa gcctacgatt
    10981 tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat gctgctcttg
    11041 gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt gttcgccatg
    11101 cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc aaactcaaag
    11161 gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc atgttttacg
    11221 atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt cttgaataaa
    11281 gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa caaatgatat
    11341 tgtctatggt gtccacgccg ttaccgaagc cctccttgca aatacaggaa acaaactcta
    11401 cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag ctacagaaaa
    11461 gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagatgactg aaggtgctgt
    11521 tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag attacatcct
    11581 tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc taactgatcc
    11641 ccataatctg ggttctatct tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat
    11701 tcccaagcac cgtgctgtcg gagtaactcc tgtcgttgcc aaaacagcca caggtgctat
    11761 tgaacacgtt ccaattgccc gagtgacaaa cctcagtcaa accttagata aactcaagga
    11821 tgaaggcttc tggacctttg gaacggatat gaacggtact ccttgccaca agtggaatac
    11881 aaaagggaaa atcgccctca tcatcggaaa tgaaggaaaa ggcatctcta gcaacatcaa
    11941 aaaacaggtc gatgaaatga ttaccattcc gatgaatgga catgttcaaa gcctcaatgc
    12001 cagtgttgct gcagccattc tcatgtacga agttttccga aatagactat aaaaaagttt
    12061 ccagtcatct gattggaaac ttttttatga ttaactatgt tctgtgataa acttgtaggc
    12121 ttcttgacca gcgatagctc catctccaac tgctgttgtt acttggcgaa ggtctttcaa
    12181 gcgaacatct ccaactgcaa agataccgtc gactgcagtt ttcatgtggt tatctgtcac
    12241 aatccatcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa gagggtccaa
    12301 tccaacatag ataaagacac caccgaaggc ttgttctgtc acttgacctg ttttcacatt
    12361 ttcaaatacg actgattcta ctcggttttc acccttgatt ttccttacta cagaatccca
    12421 tataaagctg attttctcat tggcaaaggc gcgatcttgt aaaacctttt gggcacgaag
    12481 ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga agagggcttc
    12541 ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga agaaagcacc
    12601 atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc caggcactcc
    12661 caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat atgtttggtc
    12721 atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat aaccataaat
    12781 gtgctccaca ccaagatttt caagtggttc aaacatcttt tcagccaatt caggtccact
    12841 aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct gaccacctgg
    12901 cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca aggccgcagt
    12961 catccctgca ggtccagcac cgataataat agtatcgtac atatagattc cttctttctt
    13021 gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta tgccttgaaa
    13081 acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac tccaatcata
    13141 atcatatcat agagatgggc ttggcgagcc ttggctgtct tatcaactcc cgacatggct
    13201 ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa tatcaaactc
    13261 tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat cttttttaaa
    13321 gagtaaactc agctagtcca actaactgag ttttccttta tctattatat caaatataag
    13381 tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg tggataatct
    13441 catccggagt tccagactcg atgattttcc ccttatctaa gaagagaatc ttatccgcaa
    13501 cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct gacttagcag
    13561 catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct gaagttggtt
    13621 cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca acccgttgct
    13681 tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc ccaaccttag
    13741 ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg acaacaacca
    13801 agccttcttt cacattatca agtgctgttc ggcgttcaaa caaattaaac tgttggaaaa
    13861 ccatagacaa cttacggcgt agggcaagga tttcttcttg agtaatttta gaaaaatcaa
    13921 ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa ttgagactgc
    13981 gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacaact tccccttttt
    14041 gaatatccaa gtccagatga tccaagacag tctgtcctga aaaggatttg cttaaattcg
    14101 aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat caggtgcaga
    14161 aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat tgactaccca
    14221 ataaacaagg gcaacggaga tgaagcgttc aaaatagcga taatcagctc cacctagaat
    14281 ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg ttcccttggt
    14341 caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat taggaataat
    14401 cactcgtcga taaacttgcg ctcgggtcat acccagactg cgtgccgcct caatctcacc
    14461 aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg cctcattaaa
    14521 ggcaaaggcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga gaccagttcc
    14581 ccattgctga ttgagggctt tcaaagccaa agggattccg tagtaggtca acatgagttg
    14641 caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg gatataaaat
    14701 cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc
    14761 accaccaatt gtcaacataa ttgttgttgg aagttgttgg acaattctag ggattccatc
    14821 aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca agttttggta
    14881 ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg ataatgattc
    14941 attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac agactttgtc
    15001 tacctactag tttatcatac tttgaaacag ggaagttata tattttatct atcaaagaga
    15061 tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg atttgcttaa
    15121 cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt gctaacaact
    15181 cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat tcacggtatt
    15241 tgattccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct agccaattca
    15301 aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag tccacaccta
    15361 attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat tcgataaatt
    15421 ctaacatgtg cttctccttc tatttgtttc tatctttaaa atctactcct tcatgctcca
    15481 agagccaagc tttcttttcc actcctgcag catagcctgt cagacgcttg cctgctccca
    15541 acacacgatg acaaggtact aggatagacc aaggattgcg tcccactgct ccaccaattg
    15601 cttgagcaga agccacttgc aggtcttgag caatttgtcc ataggtcact gtctgaccat
    15661 aaggaattcc ctgtaaatag gaccaaactc tcttttcaaa attcgttcca attggcgcca
    15721 agagcaagtt ggataaatcc tgaggctcgc ctttaaagta atcatctaag caagcaataa
    15781 ctgggtctaa aataggatga ctaacaactt cttctatcgt ttcatctcct agtcccctct
    15841 caaaatgctt ctgctcctga acccaaattc catacaaata atggtcatca gctacaagtg
    15901 atagaatacc aattggtgag gagtagagga tttttacata cttcttttgc attatttctt
    15961 taatttttga taggccaagc gttcgccatc tactggcacc ttaccaactt gtttaaaacc
    16021 aagtttttca aaaatatgtt gcataacctt gttttcagca tgcgtatctg agcgaaaatc
    16081 aagataatca aaaccttcaa tcaagccctc taagaaggtt tgagcaactc cttttccctg
    16141 cacatctgct gccacagcaa tacggtgaaa gactagatac tctgactctc cagcttgcca
    16201 gtttccctca taaatagctt cataggcctc ctctggactc ttggtcacag cagcataggc
    16261 tagcagttct ccctcttcca aggctacgta ggcttgccct gagatgatat catcgataat
    16321 aacatctgca tttggatagc cattttgcca ctggttacta ccagcatctg ctaaacattt
    16381 tttggcatcc tctatcacct gcatgatggc atctacttca tttggaaaag ctaaacgaat
    16441 ttccatcttt tttcctcatt tctgactagt ttctcccatc atagcataat ttaagtcttt
    16501 ctacaagcag ttaaaacttg actttgtatt tttgttattt tataatctag ttatcaaaac
    16561 tatataaaaa ggagttgaaa atgaaaacta ccttttccta cccaaaatgg gcagaaattc
    16621 caaacattaa cctctatctg gaccaggttt tgctctatgt caatcaggtc tgcgccccta
    16681 tctctcctaa taaagacaaa ggcctaacag catctatggt caacaattat gtgaaaaatg
    16741 gttacctgac aaagcctgac aaaaaaaaat accaacgcca acagattgcc cgtttgattg
    16801 ctatcacaac cctcaagtct gtattttcta ttcaagaaat agcccagaca cttaatactc
    16861 tacaaactca agcaagttca gaccaactct acgatgcttt tgtggactac atgaaccaag
    16921 gaattgaccc agctaaccct attatccaaa ccagctgcca aaccgttaaa ctctatcatc
    16981 aaactctaga cttaatcgat catactcaag aggaggtaat ccaatgaaca ctagtcttaa
    17041 actcagcaaa caactcagtt ttggagagga gattgctaat agcgtgaccc atgctgtggg
    17101 tgcagtcatc atgcttatct tgctgcctat ttcatccatc tatagttatg aagcacacgg
    17161 atttttatcc tctatcggcg tttccatttt cgtcatcagt ctctttctca tgttcctatc
    17221 atccaccatt tatcactcta tggcctatgg ttcgacccac aaatatgttt tgcgaatcat
    17281 tgaccattct atgatttacg ttgccattgc cggctcatac acgcccgttg tcttgacctt
    17341 gatgaataac tggtttggct atctgattat tgtcatccaa tggggaacga ccatctttgg
    17401 tattctctat aaaatctttg ctaaaaaggt caatgagaaa tttagccttg ctctttacct
    17461 gattatgggc tggttggttc tggctatcat tcctgccatt atcagtcaaa cgacacccgt
    17521 tttctggagt ctcatggtaa ctggcggact ctgttataca gttggagctg gattttatgc
    17581 caagaaaaaa ccttatttcc acatgatttg gcatctcttt atcctagctg cgtccgcact
    17641 tcaatacatc gctattgttt attacatgta aaaaagttga gaaattcaat ctcaactttt
    17701 ttctttacac atattgataa agtactggtg caagcgcaca tcatcagtca attctggatg
    17761 aaaagaactt accaacatat ttttttcttg ggctgcaaca atttgattgt tcactgttgc
    17821 tagaatttct acaccctcac caacactact gataatcgga ccacggataa aggtcattgg
    17881 aatcttgcca actcccttac attctgcttc cgtgtagaaa cttcctaatt ggcgcccata
    17941 agcattacgc tcgaccacca tatccatagt tcctagatga ctctctttct gagaagtgat
    18001 ttccttagcc agcaaaatta agcccgcaca ggtcccaaac actggtaagc cagatagaat
    18061 gccttctcgg atgggaagta gcatgttctg gtcacgtaag agcttgccca tggttgtaga
    18121 ctcaccacca ggcaaaatca aacccgacaa gtcactctga tcttgctgaa aatcatctag
    18181 atttctgagt tctacactct cgacacctaa ttgatctagc acttttgcat gttctgcaaa
    18241 ggccccttgc aaggccaata ttccgatttt catctatttt cctcgttcag ccatgagaat
    18301 ttggatttca ttttcattaa taccaaccat ggcttctcct aaatcttcag agatttgagc
    18361 taggatttga ggattacgga agttagtcac agccttaaca atggcactcg ctcgtttaac
    18421 aggatctcct gacttgaaaa tacctgaacc gacaaagacc ccctctgccc ctaattgcat
    18481 cattaacgca gcatctgctg gcgttgcaac acctccagca gcgaaattta caactggcaa
    18541 ttttccatgt tcatgaacat attggaccaa ttctacaggg acttgcaaat ccttggcagc
    18601 aacataaagc tcgtcctcac gtaagttttg aatgcggcga atttcctgat tcatcatacg
    18661 catatgacga acagcttgga cgatatcccc tgtccctggt tctcctttgg tacgaatcat
    18721 ggaagcacct tcagcgatac gacgcaaggc ttcacccaaa tccttagcac cacagacaaa
    18781 aggaacttgg aattctttct tgtccacatg gaaacggtca tcagctggag atagaacttc
    18841 actctcgtcg atataatcaa tttcaatagc ctctaaaatc tgagcttcaa caaaatgccc
    18901 gattctgacc ttagccatta ctggaatact aaccgcttct tggatttcct taatcatctt
    18961 tgggtcgctc atgcgggaaa ctcctccagc tgcacgaata tcagccggaa ttcgttccaa
    19021 ggccatcaca gctgccgcac cagcagcttc tgcgatacga gcctgttcag gattctgcac
    19081 atccataata acaccaccct tgagcatctg tgccaagttt ttatttagtt cataacgatt
    19141 ttcagtcatt tgttttatcc tcatcatttg tattggtagc taccttttca ttttaagtca
    19201 gaacagaatg aagcacaaga taaaaaaact ataattgtat ggggacagat tagctaaaaa
    19261 ctatctgacc ttaacttaag gccagatagc tcattcattt ttatttttca gccgtaaggg
    19321 cagccattgt gatgtagttg tatggtttgt tgaagtgtgg caagaagaag aggtctgtca
    19381 atgccaattt atcaattgtc acatgctctt ggatagcaag tgagaacatg tggattccca
    19441 tgctaattgc aatatcatgt gaaaccattt gggcaccaag aatttcacgg ctatctttgt
    19501 caaagacaat cttaattgct acttcatggt tgtcatgttt catgaattct ggtttttgaa
    19561 gatcgttaaa gcctgtttca gttgcgttgt aaccagcagc tttcgctttt tcaagagtca
    19621 aaccagttga aaccatgtga agaccgtaga ttgagatacc atttgaacct tgaacaccga
    19681 ttccttccaa ttcatgtcca caagcgttgt aggcaccaac gataccagtg cgaacagcat
    19741 ttgaagcaag agcgatatag cttgtatctt tacgagcatt gtcataaaca gtcgcacagt
    19801 caccaacagc gtaaacgcct gggatagatg tttcttgttt cttgtctaca aggaaggcac
    19861 cgttgcggaa gagttcgatc ttaccatctg caagggctgt gtttggacgg aaaccaactg
    19921 caaggataac catatccacg tcaaagcttt ctttgtcagt aatcaagcgt tcaactttac
    19981 cgtcaccttc gattgcttta acagtttgac ctagagccaa gcggatgttg tgatcttcca
    20041 agttcttcgc catcatttgt gtgaagtctt tgtcatagta accgttcaag acagtatcaa
    20101 cgatatcaac aaggacaact tcttttccaa gacgttcaaa ggcttcagca agttcaacac
    20161 cgatgtaacc accaccaaca acggcgatac ggtcgaggtg ttggctcttg tcagaaagtt
    20221 tattgataac ttcttcagca ttttggtaca atttcacgaa ttgtacgttt tcaagagttg
    20281 ctttaaattc gcggtttcct ttaacaattt caacaccttc gattggtggc aagattggtg
    20341 tagaacctgt agcgaaaatc aatttttcgt atgattcttt gtgctctttt ccttcaactt
    20401 ccgctgtaac tactttgtta tcatagtcga ttgaaagaac aggtgagttc atgtaaactt
    20461 tagcaccttt agcttccaat ttttctttat cagaatagaa caagccttca gcaccgtcaa
    20521 tttgttcacc aatccaaaga gccattccac atcctaggaa agagatgtta gagttttggt
    20581 caaatacaac aatttcgttc tcatttccaa aattatccaa catggtattg atacatgctg
    20641 taccagcgtg gttagcaccg actacaacga ttttactcat aaaaatttcc tgccttcatt
    20701 ctaaatttac cttactagta taccatttac cgttagcgtt tacaaggggg gtactattat
    20761 ttcagcattt tttatcaaaa ataattcccc atttatcctg ttttcaagcc tcatgaattt
    20821 tatgagcata tttcttgact ttttccaaat tttgtttgtg aaaattctaa ctttatggta
    20881 ttataataac atgaaaatga aatgtaaggg cttcaattta gatgattatt tgatattaca
    20941 acttattttc tataagagaa aggagttggt agcttgactc ttcgttcaca ttctgaacgg
    21001 ctaatgggga ctactatcac tatttcatta gtggatgagc aagccgatat ctttctccaa
    21061 aaatcctttg acttgctcaa agaacttgaa taccgcttca acgccaatag tcaagaatct
    21121 gagttgatgg aaatcaatta tcaagcagga atagcccccg tcacggttca tccagacctg
    21181 tttgagctga tttcacttgg gttagagcat agcctagcgc tctctagcca cctcaatatc
    21241 agcattggtc ccttaattca aacctggcgt atcggttttt cagatgccaa ggtcgcccag
    21301 cctcaagaaa ttgaatcggt gctgcctcta atcaatcctc atggtatcga gttagattct
    21361 tctacttcta ctgtgttttt aaaacagaaa ggaatgaaga tcgatctagg ttgtttagcc
    21421 aaaggataca gtgcggataa ggttgcccaa tttcttagga aagagggggt gacttctgcc
    21481 ttgatcaatc tgggagggaa tatcctgacc attggaaaaa atcaggcaag aggggataac
    21541 ccatggcaaa tcgggattca agacccagcc aatcctaggg gaaatcattt aatgaccatc
    21601 cctgttgtca ataaatctgt cgtgacttca ggcatttatg aacgtcacct gaccgtcgat
    21661 ggacaagatt accatcacat ttttgacagt caaacaggat atcctgttga aacggaacta
    21721 gcgagtctaa caatcatctc tgataaatca gtcgatggcg aaatctggac gactcgactc
    21781 tttggagaaa gacctgcttc tatcctctgg caagtcgaaa gtttggaggg catcgaagtc
    21841 atcctcatcg ataaagaagg ccacctaagc tgttcttcag gaattcctac tctatagaga
    21901 caactgtttc aatggaattt taaaattgta ttatgtaaaa agagaaagga aatctcatgt
    21961 taaaacttat tgctattgtt ggaacaaatt caaaacgttc tacaaaccgt caattgcttc
    22021 aatacatgca aaaacacttt gctgacaaag ctgaaattga gcttgttgaa atcaaggcca
    22081 ttcctgtctt caacaaacca gctgacaagc aagtacctgc tgaaatattg gaaattgctg
    22141 ctaaaatcga agagacagat ggcgttatta tcggtactcc tgagtatgat cactctattc
    22201 cagctgtttt gatgagcgct cttgcttggt tgtcttatgg tatttaccca cttttgaaca
    22261 aaccaatcat gattacaggt gcttcttacg gtacgcttgg ttcatctcgt gcccaattgc
    22321 aacttcgtca aatcttgaat gctcctgaaa tcaaggcaaa tgttcttcca gatgaattct
    22381 tgctctcaca ctctcttcaa gcatttaacc caagtggcga cttggttgac cttgatgtta
    22441 tcaagaaatt ggatgccatc tttgatgact tccgtatctt tgtaaaaatc acagaaaaat
    22501 tacgtaatgc acaagaatta cttcgcaaag atgctgaaga ctttgactgg gaaaatttgt
    22561 aagataggag accaaaaaga atgaaatttg ttggacttgt tggatcaaac tacgatcaat
    22621 catataaccg taagctcttg gaatttatcc gtcgctactt caaactcaaa tttgaattag
    22681 aagttcttga aattgacgaa gttccaatgt ttaaccaaga tgaaaaatgg gatgaaagtt
    22741 tccaattacg ttatttatat aacaaaatta ctcgtgcaga tggtgttatc attgctactc
    22801 ctgagcataa ccacactatc tcagcttcac tcaaatctgt acttgaatgg ctttcatacg
    22861 aagttcatcc atttgaaaac aagcctgtta tgattgtggg tgcatcatac tatgaccaag
    22921 gaacatcacg tgctcaagtt caccttcgta aaatccttga tgctccaggt gttaatgcct
    22981 acacgcttcc aggtaacgaa ttccttcttg gtaaagctaa ggaagctttt gataacaacg
    23041 gaaatatcac caacgaaggc actgttaaat tccttgaaac ttgcttagat aactttgtta
    23101 aatacgtagg agtcgtttcg aaattgaaaa aaccaaaacc aatcgaatca gaagacttgg
    23161 attgtggaaa accaatcgct acaaccatca cagaagttga ccctgacgat ccagaatggg
    23221 tagaaaaagt tgcagcaatc actggtgctg tttctggtga tacctatgtc aaattggacc
    23281 acggtatcct aacagttaac caaattgata tgttcttgaa agctatgcca tttgaattga
    23341 catacgctga cgataacaac caattcctct actacaacaa cgctcaccaa gatccagaca
    23401 ctatgttcgc taaacgtgta ccacctcaat caggtagccg tatgtcgact gttcatggtt
    23461 ctcttccacc agcacgtatg aagaacgtag agtgggttat cggaacactt cgcaacggaa
    23521 accaagaata cgtccgtacg atcgttccag gttctcctgc aggtgttatc aacactcaca
    23581 actaccaagc aatgtactat cctgatggat catacgctgg tatcaatgaa atcgtcttta
    23641 acttccaacc atggcttgac tggtacctaa aagagactgg tcaacgtttg gtgggtggta
    23701 gcggaccatt tgctcctgct gccggaggtc atggagacgc cgatgctact tctggcgctt
    23761 ctgattcagg ggatgctgga ggccacggtg gtgacgcaga cgctacagcc ggtgcaagtt
    23821 actaatgata gtttttagga accattatgg ttcctttttt agcgaaaaaa gactagcaaa
    23881 cacaaactgc tagtctcttt gttatcgttt catctatctt tgttaaaaga aagaatacat
    23941 attatgaatt tggatcatca agactacggc catgtaatcc tttttcacgt tgtacttggc
    24001 gtagtttttc tggtgtaaca tcgtttcctt cttcgtccac aattttgatt ccttcaatgt
    24061 ggtgacgaac agcgcggcga taaccttcga tgtactcctc acgtagtttg gcttgttcca
    24121 ctttttcttc tggtgttaag ccttctgttt ttttcttttt agcaagctca ttgatacgag
    24181 caattttttt cggatccatt tcttctcctc tgtaataaga taaagtccgt ttatcaaatt
    24241 ttatttagtg ttaatttggt taaaacaagc aaacttagct gctttcttgg ttagttgtga
    24301 caagattgcc aaacgatttt gacggacagc ctgatcttca gccattacca tagtattttc
    24361 aaagaaagca tcaatgactg ggctaagcgc aaagagttgt ttcaattgct gacttgcagg
    24421 tcctgataaa acgagtgttt ctactgcttc tgccaaagct ttttcttggt cattctcaaa
    24481 tagtgctgaa tcaaccgtag caaccccttc tgccttctca gccaggttaa aggcacgaga
    24541 aagtgattca acagatggtt taaaatcttc ttccttgctt acttctacga gagcacttgc
    24601 tgcttccaac atatctgcca caacaaagtt tgaacctgca agaactgctt ctttgatatc
    24661 ttttggagta gagcccatca tcttatcaac acgagccttg ataaagtcca taacctctgc
    24721 tttattttca taagtcaaac tgtcaaattt caatgcataa aggctatcaa tcagctcatc
    24781 catagcaatg tgccaaccaa aggcatccaa gatacgaacc acaccttgag ttgcacgacg
    24841 aagggcataa gggtcattag aacctgatgg aatcaatcct actgagaaga aactcaaaat
    24901 cgtatccaat ttgtctgcaa tggctagaac tgcgccgacc ttgctctctg gaagttctcc
    24961 ttcagctgat gtaggcatgt agtgttcacg aatagcagct gccaccgctg gagtttcacc
    25021 agcaagaagg gtgtattttt cacccataat tccttggagt tcgtcaaatt caccaaccat
    25081 acctgtcaac aagtcaaact tgtaaatggc tgctgcacga gcaaggtcaa ctgtttcatc
    25141 cactgacaaa ccagcttttt ctgccaaaag tacagtgatt tgacccgtac gaatcatgtg
    25201 ttcacgaagg gaaccaatct tctcatggaa ggtgacattg tttaattttt caacaagatc
    25261 tgaaatcacc aatttttggt cttcacgcca gaagaattct ccgtcttcca agcgggctac
    25321 caagactttt tcatttcctt tgatgacatt tttcaaacgc tctgcgtttc cgttacgaac
    25381 agaaatgaag tttggcaaga gttttccatc ttgatcacga acaacaaagt aacgctggtg
    25441 ttccttcata gaagtcacca agacttcttc tggaacttca aggtatttag catcaaaact
    25501 tcccatgaag gcagttgggt attcaaccaa attcaagact tcattcagca aatccgcatc
    25561 aatttcgata cgtacaccat gttttgcctc aatttccttg atttggtcaa caatcatttg
    25621 ctcacgttca catggatctg cgattacaaa ctgcttacga aggtcttctt catagctcaa
    25681 tgctgactga attttggttt ctttccccaa gaaacgatgg ccacggctca cacgacttcc
    25741 cttgatatca aggaaatcca agtcaaactc ttgctcatcc aagagaacag ttaaagtgtg
    25801 gacagggcgg atgtattcaa agctatttcc agcccagtgc atgctgacag ggaaagtcaa
    25861 tgacttcaag acatctacaa caccaggaac aatggcttca actgcttgac caacttcttc
    25921 cttagtgaca tagacatatt cttcaccctt gatttcacgg aattcgatat cttcaacagt
    25981 caagcctttt ccacggacaa atccttgagc tgctttggtg aagtttccat cactatccaa
    26041 ggcaattttc tttgctggtc ccttgaaatc ttctgtcaaa tcagactgtt tgtctgataa
    26101 accagtcaca cgaacagcca aacgacgtgg tgttgagaag gtttgaatgg cttcaaaaga
    26161 caggcggttt tccttgagga aggctgccat tttttcgcct agttgttttt cacttggtgt
    26221 gacaacatag gctggtaatt cttcaagacc gagttctact aataagtttt ttgtcatgtt
    26281 ttttctccga aaagtatctt tttattttcc agtttgcctt atgtgattgg cacgcaagca
    26341 accaacttcg ttgtttcatt gctaaaattg gagccaaaag tctccaattt tgcctagtat
    26401 ccttagtttc actaaggata ccttcatcac tgcggcaact ggctcagggc tcactctgtt
    26461 cgcccatttt cgtaatttgt cactctcttt attctgcgtc ttctgctaaa agtttaactc
    26521 gtgtttcttc atccaaaagt gggtaaccta ggcgtttgcg ttctgcaaca aaggttttgg
    26581 ctacaacacg ggccaagtta cggatacgag cgatatagcc tgcacgctct gttacagata
    26641 cggcaccacg cgcgtcaagc agattaaagg tatgtgaaca tttgagaaca tagtcatagg
    26701 cagggtgtac caagccttct tctaatgcac gaccagcttc tttttcaaac ttatcaaagt
    26761 tttcaagcaa catttcttgg tccgaaattt caaatgaata ttttgagtgc tcatactcag
    26821 gctggataaa gatttctccg tattttacac catcagccca ctcgatatca tagacagagt
    26881 ctacttcttg aatgtaagaa gccaagcgct ccaaaccata ggtaacttcc gcagtcacag
    26941 ggccagttgc caatccaccg acttgttgga aataagtgaa ctgagtgatt tccattccgt
    27001 caagccaaac ttcccaacca agaccagctg aaccagttga tgggttttcc cagttgtcct
    27061 caacaaaacg aatatcgtgc tccaaaggat tgattcccaa tttttccaaa gactcaaggt
    27121 aaagttcttg gatatttgat ggagaaggct tcatgaccac ctggaattgg tggtgttggt
    27181 agagacggtt agggttttcc ccataacgac cgtcagcagg acgacgtgat ggctctacat
    27241 aagctgcatt ccatggctca ggtccgatag cacgaaggaa agtgtaagga ctcattgttc
    27301 ccgcaccttt ttcattatca taagcctgca taagcataca accttggtca ttccaaaatt
    27361 gttgcaaagt caaaataatt tcttgaaatg ttaatttctt agacattgaa tactccttaa
    27421 taaaaaataa ttacttgcga cacgagtctg cctcgtcgat catacgaggc aagacgagtt
    27481 agtactgcgt ctagctcagg cactctagtg ctgagcgtgg ggcagtccga ctgtaaggag
    27541 gtttctgcca ctgatgcagt gaaatgttaa tttctcagac attgaatact cctttagttt
    27601 ttatatttct ttttcaatta atttctgctg aacaaaccaa tctaacaagg gaaaagtttt
    27661 ttctgtattt gcccataaat gataagaatc aaagaccgct tgtacactac ctaaattttc
    27721 taccaactta tctactgtca aaaagctgcg ccagtattca taaaaaatac ttgcatagtt
    27781 cttatcataa gtcgaagaac caaaatcatt caatgaatgc caaccatact tcttctgaaa
    27841 aagttctacg agagattgat tacaaatttt ttccgcttga aattcctctt ctgtcaagaa
    27901 atacttgcga ctaatatatt caaccatccc ctcttcgaac cagatataag agtcataacc
    27961 atcaaaatca tctaaaaaat gctccgacca atgagctaac tcatgtccta caatctgtaa
    28021 gaggaaattt tcagataaag aatgatagtg gctttctatt gcttgagttt gctcagaaca
    28081 ctcgtagtcc atcaactgat acaaatacaa ctctttccaa acagctaaat caggcgtcat
    28141 aaccattcgt ctattatttg tataggctgg aacagcactt tccctaataa tctgtgtagc
    28201 agcattaaga tctgaccaaa taacggcttg cggtaaatca cagactgcaa gttcattctt
    28261 taaaaaagat tgataatctt tcaacttttc agtatttctt actacaaaat cacgaaatgt
    28321 agctagttga ctatcgtctt ttacaagata aaggttgtcc acggctctcc tttctttcac
    28381 acaatagact gaataagcaa aagaaccaca tctgacaagc ctaggttgat ttaacctagg
    28441 ggcgtcagaa acgcggttcc accctaattt attacttcat tgtttttgaa aatactacag
    28501 aaagcgccag cccattattt tgccctactt ggctcccact gtcccaagct cgcttgaaga
    28561 acgccataag actttctttc ctgctgacta ttatatcaaa aattagaata atgtacaatt
    28621 ctttttatga ttttctagaa atccacatca tcaactcgtg gagcacccaa ttgaacagca
    28681 atcgttttta aggtttctct ctccttatga ctcagttcaa ttccaaagca atcaagatta
    28741 gcctgaatac gagagattgt gatagatttt ggaagtggta aaaatccttc tgccaagctc
    28801 caggccaagg ctatctgagc aaccgatttt ccgtgatttg ctgctatttc ttggacttgc
    28861 ttgctatcaa acagttctcc ttgtccaaaa ggcccccaag cttccaataa aattcccttt
    28921 tcacgacagt aagctacgac ttgatcttga tacacacctg gcgccaagcg aacttgattg
    28981 accgcaggaa cgatagttgc agtttcaagc aaggcatcca aatgatgggg aagaaaattg
    29041 ctaacgccga tagcacggat tttcccttct tgatagaggt cttccatcgc tctccaaact
    29101 tccgcattgc gagttttcca tgcgtcattt tctctgagcg gttttgggtt cggccaatga
    29161 atcaaataca aatccaaata atccaagccc agtttttcta tagatttttc caaagcttga
    29221 cgagtttgct cataggtttg ctgactattc caaagcttgg tagttacgaa catttcttca
    29281 cgtggaactc cgctatcttt gattgcttga ccaacacttt cttcattctg ataaatcgcc
    29341 gccgtatcaa tatgacgata accagccttc aaggcttcta acactgcacg ataggcttct
    29401 tctccatcct tagccttaaa agttccaaat cccaatactg gaatttctac tccattattt
    29461 aattgatagg tattcatatg cttctccttc tattttattc atcaattata gcataaaaca
    29521 ttttttaagt agtactatct tctgtaaaaa ataagtctgt caaatatcta acagacctat
    29581 tttttcttgc ttattcatca cgactatagt acagctcatg agcttttagg cgagtattga
    29641 gcatctctgg tatggtcaca aaggtataac cttgattttt caaatactca atgacccttg
    29701 gcaaggcatt gactgtcgga ctgtgaatat catgcatcaa aacgatagag ccgttagcta
    29761 cttgatgctg aatttctgtc aaaatagatg cttcattttt actcttccag tccagactat
    29821 ccacatccca catgataaag ctcaaatcca agctattgcg aatatcatct gtaatagcac
    29881 cataaggtgg acgcatgagt ttagaactag aacccagcac tttagttagc acatcctcag
    29941 tatcagtaat ctgcttttta gcttcatcaa gagagagttg cgagagaatc ggatggctcc
    30001 aactatggtt tccaacaaca tgaccttcag attttatcct cttcaccaag tcctcattcc
    30061 cagaaacatt tttcccaagc acaaagaaag tcgctttaat atcatattta gctagggtct
    30121 ctaatacctg cggggtcgtt gctggatttg gaccatcatc aaaggttaga gcgacaactt
    30181 tttgatgttt cttatcaaag taagattggt acaaggccgc atctttttcg agtaagtacg
    30241 aagattggat aacatcaaag aaagcagata ctggcaaggc tatctcttct aaattttcaa
    30301 ccacaggact tggataaagg ataatctgac tatccttgta atcaaaattc catgcagaca
    30361 agtcttggtc agagaagttt tttacaatct gctcactctg gtcttgctct atttttttat
    30421 cctctatgaa ggaggtcaac tcttttatca gctgttcctt agccttacta gcatctgaaa
    30481 acagttggtc aagtgtaaaa ggttgcccat cttctgttaa gtgaaccttt cctagactgg
    30541 tcttttcagt ctcttcaatc ttaaaagcaa ctaaatcata gatttgtttg gtcacattac
    30601 gattaacgac tccctttaaa cctgactctt cttgctctgt atagtagaaa acaagattgt
    30661 ccttactttc tagcttgtcc ttaacatctt gatttatcag ctccctaaca gaggaaatca
    30721 ctttctcccc ttggagagga taataggcaa tcacttcggc ttgcccctga cgaaaatgct
    30781 ccttctgatt tccctcactc aattgatcat ctttctcttt tttgagcgat tcaatctttt
    30841 gctcaaaact tttttgctgg tagatcttaa aagcaataaa tccgcctaat aggcaaatag
    30901 aaattgctaa aataccaatc aaagccaata atacatgtct cgttttcccg tgtctgccac
    30961 gtcctagtct acttttattc atatttatat cataacaaat ttgagcaata atttcaatga
    31021 tacaatttgg caatccacaa ttcctctagc aagccaaagc atcttacatc aactccaaca
    31081 tccgtatctg tagagattat gtagttttct atctatggaa tttctagtag attgaagcta
    31141 gaatagtaga cctatacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
    31201 ttgtcctgtt ctcatttcat ttcactataa aactatgaaa agctaggtca tctgagaagt
    31261 aattactatc gacaattgcg atagcgtata ctagtgcgat ttgtttgtag ctctggtggg
    31321 tatcaccttc acgttggcga attttttcta ggttttgatt aacctgactg cacagataag
    31381 cccataggcg attgatgaaa aaattctgat gatgaacctg aatttcgatg ataacttgga
    31441 ttccattatc taactccgcc aagacgtcta tactagtata gaaatcttgc gctgagtagg
    31501 gcatggaagg caagacgtga atgttacttc cctccaaaat cgtcacattt tttgctggca
    31561 agtccagcat atcgcggata aattgacaaa tcattagttg ggctgatacc tggatgtctg
    31621 agaatcctcc ttttcctcct tctattatag cacattttta aatctaggtt tgctagattc
    31681 tatgcttcta tctatttatt cggaaaagaa agctaatagc ttatttgaaa ttttctcctc
    31741 tctgttggat agtcccgtct atctatgtta taatgaaaga aggatttaaa atcggaaaag
    31801 gagtatttat gctaaaatta ggtgtcatcg gaactggcgc tatcagccat catttcatag
    31861 aagcagccca taccagtgga gaataccaac tggtcgcaat ctattctaga aaactagaaa
    31921 ctgcagcaac ctttgcttct cgctatcaga atatccaact attcgatcaa ttagaagtct
    31981 tcttcaaatc ttcttttgat ttagtctata tcgccagtcc aaactcctta cattttgctc
    32041 aggcaaaagc tgccttgtct gctggtaaac atgttattct tgaaaagcca gctgtcactc
    32101 aaccacaaga atggtttgat ttgattcaaa cagctgaaaa aaataactgt tttatctttg
    32161 aagcagctcg taattatcac gagaaagcct ttactactat caaaaacttt ttagcagata
    32221 agcaagtgtt aggagcagat ttcaactatg ccaaatactc ttccaagatg cctgacttat
    32281 tggctggaca aacaccaaat gtcttttcag accgttttgc tggtggagcc ctcatggatt
    32341 tggggattta tcccctctat gctgctgttc gtctttttga aaaagctaat gacgcgactt
    32401 accatgctca acagcttgac aatagcattg acctaaatgg agatggtatc ctcttctacc
    32461 cagactatca agttcacatc aaggctggaa aaaacatcac ttccaatctt ccttgcgaga
    32521 tttatacaac agatggaacc ttgactctca acacgattga gcatattcgc tcagctattt
    32581 ttaccgacca ccaaggaaat caagtccagc ttcctatcca acaggctcct catacgatga
    32641 ctgaggaagt cgctgcattt gcacacatga tccagcaacc agatctgaat ctctaccaaa
    32701 cttggctgta tgatgcaggc tctgttcatg agctactata taccatgcgc cagactgctg
    32761 gcgttagatt tgaggcagaa aaatgaaaac caaactaccg actgaatggc aagaactgag
    32821 tgaccaactc ggtttccaag aattcacccc cattcaaact caactatttg agctccttct
    32881 tgctggagaa aacctcctag gagtgagcca aaccggaact ggtaagaccc tagcgtatct
    32941 cctaccaagt cttctcagac tacaaaagaa aaaagcccaa caactcttga ttctagcacc
    33001 aaacacggaa cttgctggac agatttttga tgtatgtaaa acgtgggcag aagctatcgg
    33061 attgactgct caactcttcc tttcaggttc aagtcagaaa cgacaaattg aacgtcttaa
    33121 aaaggggcca gaaattctga ttggaactcc tggccgtatc tttgaactca ttaaacttaa
    33181 aaaaatcaag atgatgaatg tggaaaccat catcctggat gaatttgacc aattactaga
    33241 tgattctcag attcactttg tagagaaaat cactcactac gcacctcgtg accaccaact
    33301 ggtctacatg agtgccacga ccaagtttga ccaagaaaag attgtgccga atacacgcac
    33361 tattgatctc tctgatcaaa aattggacaa tatccagcat ttctacatgc aggtagacca
    33421 acgtcatcga gtggatatgc tacgaaaact ggctcatgta gaggatttcc gtggtctagt
    33481 cttcttcaat agcctgtcag accttgggaa tgccgaggaa aaactacagt atcgtgatat
    33541 attggctgtt tccctcgcta gtgatgttaa tgttaaattt agaaaaatca tcttagaaaa
    33601 gtttaaagat aaccaactaa ccctgcttct tgcaactgac cttttggctc gtggaattga
    33661 tatcgatagc ctagaatgtg tcgtaaactt tgatattcct agagatagcg aaacctacac
    33721 acaccgtgct ggccgtactg gtcgtatggg caaggaaggt tatgttatca ctctcgtcac
    33781 tcatccagaa gaaatcaaaa aacttaaaaa gtttgcaagt atccgagaaa ttgtcctaaa
    33841 aaaccaagaa ctctatatca aataaagccc cattgctcta tacacatcca gtgtatggag
    33901 cttttttata agcaattcaa ttttatactc atctgctttc aaaaagcatt ctagtccatc
    33961 tccgattaac gatggacttt atcacctcct tctccaatcc ttgtatgaca tcttgaagtt
    34021 gattcatgac atcttccaaa attcgaaagg c
//
LOCUS       DBERCA010000016        33068 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.016, whole genome shotgun sequence.
ACCESSION   DBERCA010000016 DBERCA010000000
VERSION     DBERCA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.016"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(210..407)
                     /gene="blpI"
                     /locus_tag="ACL719_000903"
     CDS             complement(210..407)
                     /gene="blpI"
                     /locus_tag="ACL719_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGS0698899.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            697..1284
                     /locus_tag="ACL719_000904"
     CDS             697..1284
                     /locus_tag="ACL719_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS0698900.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1211..2854
                     /locus_tag="ACL719_000905"
     CDS             1211..2854
                     /locus_tag="ACL719_000905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HGS0698901.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDVLASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLENSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFKPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILKAYELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2865..4226
                     /locus_tag="ACL719_000906"
     CDS             2865..4226
                     /locus_tag="ACL719_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGS0698902.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANYLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            4283..4438
                     /gene="blpC"
                     /locus_tag="ACL719_000907"
     CDS             4283..4438
                     /gene="blpC"
                     /locus_tag="ACL719_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGS0698903.1"
                     /translation="MDKKQNLASFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPIQL"
     gene            complement(4483..5823)
                     /locus_tag="ACL719_000908"
     CDS             complement(4483..5823)
                     /locus_tag="ACL719_000908"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0698904.1"
                     /translation="MNIVWILLHTLITNGLEIVIFFKVDGINLTFERIFKAFLLKILL
                     VFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDIERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLKDSSKKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(5837..6574)
                     /locus_tag="ACL719_000909"
     CDS             complement(5837..6574)
                     /locus_tag="ACL719_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0698905.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETDLLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6579..6917)
                     /locus_tag="ACL719_000910"
     CDS             complement(6579..6917)
                     /locus_tag="ACL719_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0698906.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNI
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7193..7522
                     /locus_tag="ACL719_000911"
     CDS             7193..7522
                     /locus_tag="ACL719_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS0698907.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7690..8739)
                     /locus_tag="ACL719_000912"
     CDS             complement(7690..8739)
                     /locus_tag="ACL719_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0698908.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8736..9467)
                     /locus_tag="ACL719_000913"
     CDS             complement(8736..9467)
                     /locus_tag="ACL719_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698909.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9535..9945
                     /locus_tag="ACL719_000914"
     CDS             9535..9945
                     /locus_tag="ACL719_000914"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS0698910.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9955..10242
                     /locus_tag="ACL719_000915"
     CDS             9955..10242
                     /locus_tag="ACL719_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698911.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10965..12101)
                     /gene="dnaJ"
                     /locus_tag="ACL719_000916"
     CDS             complement(10965..12101)
                     /gene="dnaJ"
                     /locus_tag="ACL719_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGS0698912.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12503..12859)
                     /locus_tag="ACL719_000917"
     CDS             complement(12503..12859)
                     /locus_tag="ACL719_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGS0698913.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12861..14684)
                     /gene="dnaK"
                     /locus_tag="ACL719_000918"
     CDS             complement(12861..14684)
                     /gene="dnaK"
                     /locus_tag="ACL719_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGS0698914.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(15164..15688)
                     /gene="grpE"
                     /locus_tag="ACL719_000919"
     CDS             complement(15164..15688)
                     /gene="grpE"
                     /locus_tag="ACL719_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGS0698915.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15715..16749)
                     /gene="hrcA"
                     /locus_tag="ACL719_000920"
     CDS             complement(15715..16749)
                     /gene="hrcA"
                     /locus_tag="ACL719_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGS0698916.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16913..17423)
                     /locus_tag="ACL719_000921"
                     /pseudo
     CDS             complement(16913..17423)
                     /locus_tag="ACL719_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            18031..20364
                     /gene="hsdR"
                     /locus_tag="ACL719_000922"
     CDS             18031..20364
                     /gene="hsdR"
                     /locus_tag="ACL719_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGS0698917.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKAHRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20377..21894
                     /locus_tag="ACL719_000923"
     CDS             20377..21894
                     /locus_tag="ACL719_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS0698918.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINAIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTNDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILPLDQGFKNRQAQTKQSPSSFIEII"
     gene            complement(21983..22132)
                     /locus_tag="ACL719_000924"
     CDS             complement(21983..22132)
                     /locus_tag="ACL719_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGS0698919.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFYKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            22097..22216
                     /locus_tag="ACL719_000925"
     CDS             22097..22216
                     /locus_tag="ACL719_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGS0698920.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(22312..23658)
                     /gene="glnA"
                     /locus_tag="ACL719_000926"
     CDS             complement(22312..23658)
                     /gene="glnA"
                     /locus_tag="ACL719_000926"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGS0698921.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(23695..24051)
                     /gene="glnR"
                     /locus_tag="ACL719_000927"
     CDS             complement(23695..24051)
                     /gene="glnR"
                     /locus_tag="ACL719_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGS0698922.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(24128..24655)
                     /locus_tag="ACL719_000928"
     CDS             complement(24128..24655)
                     /locus_tag="ACL719_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS0698923.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDKSVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(24791..25987)
                     /gene="pgk"
                     /locus_tag="ACL719_000929"
     CDS             complement(24791..25987)
                     /gene="pgk"
                     /locus_tag="ACL719_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGS0698924.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(26077..31017)
                     /locus_tag="ACL719_000930"
     CDS             complement(26077..31017)
                     /locus_tag="ACL719_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGS0698925.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANPELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFALSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDNLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVFSDSKTGVEVHFSN
                     KEKPVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            31310..32941
                     /locus_tag="ACL719_000931"
     CDS             31310..32941
                     /locus_tag="ACL719_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGS0698926.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGFGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT        10065 a         6900 c         6067 g        10036 t
ORIGIN      
        1 taaataatag tttatagtta agttttttat tcctaccaat ccatcaacta agtaaagcat
       61 caacgattac ataaacgatt gataatataa ttaaaatttt gctaactatc ttattctcat
      121 cattcttaga taactttgat attttgtaag taagtaaata agacagtaaa ttaatagcga
      181 taataatact atatttaaga atcataatct tacaaagagg acataattcc tgaacctaca
      241 caaataagtg ttgctgctcc cccagttatc ggaccagtcg cagcagctaa tagtactgct
      301 ccaatacaac caccgattgc agatcctaaa ttgcctcttc ctccactaac tatttcgagt
      361 tcttcattat ccataacaga aaattgttcc atcatttttg tattcatgac aaatactcct
      421 tttttatttt tgtcttgttg taactttgat aagtttagta tatcatcgtt ttttaaaatt
      481 tttcatccag atcttgaatt gtcatcgaaa cgtcttgaat tagctttttt atttcaagcc
      541 acctctattt caagccacct ctaaatgttt aaaaaaaata atttctaatc acttttttac
      601 cattcaggaa gttttaatga ctattcaaga tttcataaaa tatgaactta gttttatgac
      661 ataatagacc tatccactat atgaaaggaa ttgccaatga cttcttataa acgtacattt
      721 gttcctcaaa tagatgcgag agactgtggt gtcgctgcct tagcctcgat tgctaaattc
      781 tatggttcag atttttctct agctcacttg agagaacttg caaagaccaa taaagaaggg
      841 acgactgctc ttggcattgt aaaagccgct gatgaaatgg gctttgaaac aagacctgtt
      901 caagcagata aaacgctctt tgacatgagt gatgtcccct atccatttat cgttcacgtt
      961 aacaaagaag gaaaactcca acattactat gttgtctatc aaacaaagaa agactatctg
     1021 attattggtg atcctgaccc ttctgtaaaa atcactaaaa tgtcaaaaga acgctttttc
     1081 tatgaatgga ctggagtagc tatttttcta gctaccaaac ccagctatca accccataaa
     1141 gataaaaaga atggtctact aagcaagctt ccttcctctg attttcaaac aaaaatctct
     1201 cattgcttac attgttctct caagcttatt ggtcactatt atcaatatag gtggttctta
     1261 ctatctccaa ggaatcttgg atgaatacat tccaaatcag atgaaatcaa ctttaggaat
     1321 catctcagtt ggtctggtta tcacctatat cctccaacaa gtcatgagct tctccagaga
     1381 ttatctccta accgttctga gccagagatt aagcattgat gtgattttat cctatattcg
     1441 ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag aaatcatttc
     1501 acggttcaca gatgctaact ctattataga tgtcttggct tctaccattc tttctctttt
     1561 tctggatgtt tctattctga ttcttgtagg gggagtcttg ctggcacaaa accctaatct
     1621 cttcctcctt tctcttcttt ccattcctat atacatgttc atcatctttt cttttatgaa
     1681 accttttgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta gctctgccat
     1741 tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta
     1801 tcaaaatata gacagcgaat ttgtagatta tttggaaaat tcctttaagc tcagtaaata
     1861 ttctatttta caaacgagtt taaagcaggg aacaaaatta gttctgaata tccttatcct
     1921 atggtttggc gctcaattag tcatgtcgag taaaatttct atcggtcagc tgattacctt
     1981 taacacactt ttttcttact ttacaactcc tatggaaaat attatcaacc tccaaaccaa
     2041 actccaatct gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag tcgaatctga
     2101 atttcaagtt caagaaaacc ctgttcattc acattttttg atgggcgata ttgaatttga
     2161 tgacctttct tataagtatg gttttggacg agatacctta acagatatta atctcacgat
     2221 taaacaagga gataaggtta gcctagttgg agttagtggt tctggtaaaa caactttagc
     2281 caaaatgatt gtcaatttct ttaaacccta caaagggcat atttccatca atcatcagga
     2341 tattaaaaac attgataaaa aagtcttgcg ccgtcatatt aattacctac cccaacaagc
     2401 ctatatcttt aatggctcta ttttggaaaa cttaaccttg ggcggtaatc atatgattag
     2461 ccaagaagat attctaaaag cttatgaatt agctgaaatt cgtcaagaca ttgaaagaat
     2521 gcctatgggc tatcaaactc agctctctga tggagctggt ctatcaggag gacaaaagca
     2581 acgaatcgct ctcgctcgtg ctcttttaac taaagctcct gttttaatac tagatgaagc
     2641 tactagcggt cttgatgtct tgactgagaa aaaggttata gataatctta tatccctaac
     2701 tgataaaacc attctctttg tagcccatcg tctcagtata gccgaacgaa ccaaccgtgt
     2761 cattgttctt gaccagggga aaatcattga agttggtagt caccaagagt taatgcaggc
     2821 gcaaggcttc taccatcatc tattcaataa ataaggagaa tgtcatgaat cctaatcttt
     2881 ttagaagcgt cgagttttat cagagacgtt accataacta tgcgacagtg ttaattatac
     2941 ctctttcatt actgtttact ttcatcttga ttttctccct tgttgccaca aaagaaatta
     3001 ctgttacttc ccaaggagaa atcgccccta caagtgtcat tgcatctatt cagtcgacca
     3061 gtgataatcc tatccttgct aattatttag tggcaaatca agtagttgag aaaggtgact
     3121 tactcatcaa atactctgaa acaatggaag aaagtcagaa aactgcctta gcaactcaat
     3181 tacaaagact tgagaagcaa aaagaaggac ttggaatttt gaaacaaagc ttagaaaaag
     3241 cgactgatct tttttctggc gaggatgaat ttggctacca taataccttt atgaatttta
     3301 ctaaacaatc ccatgatatt gaactgggta tcacaaagac taacactgaa gtttcaaatc
     3361 aagccaatct tgccaatagc agttcatcag ctattgaaca agaaattaca aaagttcaac
     3421 aacaaattgg agaatatcaa gagctgagag atgctatcat aaataacaga gcacgcttac
     3481 caactagcaa tccacaccag tcaattttga atcgttatct tgtagcctca caaggacaaa
     3541 cacaaggaac ggcagaggag ccatttttat ctcaaattaa tcaaagtatt gcaggtctgg
     3601 aatcatctat cgcaagcctc aaaattcagc aagctggtat cggaagtgta gcaacttatg
     3661 ataatagttt agcaaccaaa attgaagtac tccgcactca atttttacaa acagcttctc
     3721 agcaacaact aaccgtggag aatcaattaa cagaattaaa agtacaacta gatcaagcaa
     3781 cgcagcgctt aggaaacaat accttaaccg ctccaagtaa aggtatcgtt catctgaaca
     3841 gcgaatttga aggtaaaaat agaattccaa ctggtacaga aattgctcaa atattccctg
     3901 tcatcacaga tacaagagaa gtactaatca cttactacgt atcttctgac tatctacctc
     3961 tactagataa aggacaaact gtaagattaa aactggagaa gattggaaat cacggcacca
     4021 ccatcatcgg ccaacttcag acaattgatc aaactcctac cagaacagag caaggtaatc
     4081 tctttaaatt aaccgctctt gcaaaactat ctaatgagga tagtaaactc atccaatatg
     4141 gcttacaagg tcgcgtcact agtgtaactg caaagaaaac atattttgat tatttcaaag
     4201 ataaaatttt aacccattct gattaatttt cagataacac tctataacta tttattatct
     4261 tatcaaaaag gagaatcata acatggataa gaaacaaaac ctagcttcat ttcaagaact
     4321 aacaactacc gaactcaacc aaattacagg tggaggactg tgggaagata tcttatacag
     4381 ccttaatata attaagcata acaatacgaa aggacttcat catccaatac aactataaaa
     4441 aataagaccg agtaacaaga actctcggtc ttattttttt catcattctg taggtatcat
     4501 agtaagcacc tgacgaaaga cttgatcttg acaggtagta tttaaactgg cattgggata
     4561 actttccaca atcttcataa cggtatagag accgacacct ctctcctccc ctttagaact
     4621 aactccaaag gagaagattt cagaaatatc gatgccctct tctttgatgg aattttcgat
     4681 gatgaaggtc tcctgtgctc cactttttaa aaaggcaatt gaaacatgag gttgaccggc
     4741 ctctgcactc gcttcaatag cattatcaca aaggatagac acaatggtta gaaaatcaag
     4801 cagtctcatc ccctcaacct gaatctcttc aggaacttcg acattaaaga caatgttctt
     4861 atctctagct tttataaatt ttccagctag gagacttttg agggcacgat cccgaacatt
     4921 caccaatcgg ccaaggtcat atttattgtc ctgcaatttt ttactagaat cctttaagac
     4981 caagtcatag acctctttta tctgctccat atcctcctct tcaataccca gacgtaaact
     5041 tgtcaagagg ttggtgtagt catggcgaaa actccttact tccttgtaaa gttcctctat
     5101 atgccgacta tagcgttcta tatctctata gcgcaagacc tgctcttggt ccagtctatc
     5161 atggagtttg tccttcaaat aggtatccaa tttcttgata acccccataa aaaataggag
     5221 gtagaacact aggatgagat ggcgaacagt cgttgattga ataccttgct catattcaaa
     5281 gtaagacaga ctttgtatca ccaaatagta agcccccatt atccagttaa tcttagtcag
     5341 ggacttttga aaacctgtat ctagaatctc ccttctcaaa ctagtaaaat catagtccaa
     5401 ccatttcaaa aaggctagag aaatgaagaa attgaaaatt attatacata acccagtgaa
     5461 tgagtagcca tcatatactt gcccttgtcc caaaaatgga agcacaaaat aggagactcc
     5521 tctataaaag agattcacca atatcattgg aaagagacca taaaagaaaa ggagtttttt
     5581 aggaagccct cttaacaata agaaagacaa gcctatgccg tacaagggtt ccataaaata
     5641 agataggtaa acatttccta ctatatagct aatcattaca aaaacaaaga ccaacagtat
     5701 cttcaaaaga aaggccttaa aaatcctctc gaaagtaaga ttaattccat ccaccttaaa
     5761 gaaaatgaca atttctagcc cattagtaat aagtgtatgc aataatatcc aaacaatatt
     5821 cataaactct cctagttcag tgtaagttat taatagcctc agacacttcc ctgaccttat
     5881 aacgcgcgat cagacaactt ccaccattgg gaaaaaaaag gagtttttct ttcttatcca
     5941 aatgcaccac atttgcagga ttgatgagaa aagagcgatg gcactgcaag agacggggtt
     6001 cctgcttgaa aacctcctct aaactcgctg taaattccag cctgtctgtc ttggtataga
     6061 gaataacacg atgggctctg ggagacgttt cgagatagta aacctcttta aaaggatatt
     6121 ggaattgggc aaattttgat ttaaagtaaa agcaatcttc cgccagactt ttactatctt
     6181 gactattggc atagaggagg tctgtctcga ttcgagattc aaactcctct gccgaaaggg
     6241 ccttatcaat atagtccaga gcagacactt ggtagcgaaa ggacaggggc ataaactccg
     6301 agtgagtcgt cacaaagaca atcagggcat aaggatcccg atcccgaatc tttctagcca
     6361 cttccagtcc cttcatctct tcatttcgaa tctcaatatc caaaaagaat agctgatggg
     6421 cccccttctc atgcacttca gccagcagtt ggtccggctt gccaaatacc tcaaaagagc
     6481 taggaatgat atgatgtgct ttcaaaagtt tctcaatcgt cgtttcaatt ctagtctgtt
     6541 gggaaaaatc atcctctaaa acaaatattc tcatcttctt actctccctc tttcaaccat
     6601 ttttgacgaa tttctctata gcgacgtctg gcatacttga cagcgtattg accttcttct
     6661 ccgagaaaaa ggattctttc ttgaaaatca atcgatttta atttatcaag attaaccaaa
     6721 caatttcgat gacatctcat caaggtttcc ccacattggt tctcaagatt acttaaattt
     6781 tgaagtatat taaaactagc ttcttctgta acaatctgta cggtatgggc tttagttgga
     6841 tgtgtttgga tatagtagat atcgtcgatg tttactttat agactgtttt ctgcgtctga
     6901 ataatcatat atttcattgg attctcctcc taagaaaaga ataacagaaa aggtctagcc
     6961 caagcacacc caatcctaaa cagtctttga aaactcctaa aaggattatt ttctacaaca
     7021 tcatgatgag cttacaacta ttttatcata aaattcactc agcgcaattc aagacatttc
     7081 aatgacaatt aaagatttga ataaaaaaaa agaattaaaa gtgataaact aacagtatca
     7141 aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc ccatgacaga
     7201 tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag cctaccaagc
     7261 atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact ctattcttgg
     7321 ttttctagcc aaggctgaaa cagtggataa tcgattcttg attgaactag aaaaatttta
     7381 ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc caactcgcgc
     7441 cgcttggcgg gcctatgacc gcttccactt tgaccaagtc aagaccaagt taatactcaa
     7501 tgaaaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta cggtaagacg
     7561 acgctgacgt ggtttgaaga tattttcgaa gagtataagt ctctacggac caaccattat
     7621 cctgtagtaa caaaaaagaa gttgagacag actgaactca acttcttttt tagtgtcgta
     7681 tcagcaatgt tagtcctgca tctgacgttt tacctgataa ggcaaataca agactagtaa
     7741 aaccaaacca gctcctagta aggctagaag ggctagtttt tcttggaagg taatcaaccc
     7801 aacaactaat tccacaagta aaacaaaact ggtcaatcct cgaactacct cctgtaagcc
     7861 tttttccttt tgccccttgt ccagcggaaa gagttgggtc aaatactggt agtcaaaggc
     7921 atgatagagg gccagcaact ggaagagcaa gaggtagtta aagagaacta ccactgctgt
     7981 cgcaatccaa gcttgctcga taaaaacctg cgccagtacg gaaagtaaga gaagacgaag
     8041 actgagagca aagaggtcgc catttcgcag ataagaacgc agatagagat tttgccaaat
     8101 cttcccaggc accttctgaa cagcctttaa aataaagtcc agataggcac gacgcttaac
     8161 gctgtttgaa attcccttga cctgcgtaaa gagggcaaag aaacgaagca agacttgctt
     8221 acgcttgctt tcttgagaaa taagatagtc ccagtccagt ccagtttcag tgaaaaattt
     8281 gctggccttt tgacaaaagt ggaaatattt tcctaccccc aataaaagca catagagcag
     8341 aaaaactggc aagccataac ccattgccaa aaataaaggc gcaaataaca gcaagaaaag
     8401 ggtctgtaca aagagccaaa agactaggga aatgccagtt tgacgcttga gatggagctt
     8461 gatttcctct tctccaacta agagaaagag cttgtctgga gcctccatat aggtggcaat
     8521 tcctccccaa agtaaaagta aaacagacgt aattcctaca aacaaaagga taggccaatg
     8581 attttcagga aaatgttgta agagttgact gtactggtag gctagaaagc ccaacaggac
     8641 aagcaggaac aagacaaagt ggtcattgag cacatagcgc agataaccaa gacactcctt
     8701 acgaaaggcc tgctttctct ttaaaaacaa gtctttcata ggtcctcctc ttttgtcaga
     8761 gccaagtaaa tatcattcaa actagcctca ggcatatcaa aggcttcgcg tagttgcagg
     8821 agattgcctt tggcacgcac ctctcccttg tgaagaatga caaaggcatc acacatcttc
     8881 tccgccgaat ccagcacgtg ggtactcatg agaatagact tgcccttttg cttctccact
     8941 tccaaaagct gaatcaaatc agaaatagcc agcggatcaa gaccaaggaa aggctcatcc
     9001 acgatgaaaa ggcttggatc caccacaaaa gcacagataa tcatgacctt ctgcttcatc
     9061 ccttttgaaa aatgaacagg gaaccagtct aatttctggt ccagacggaa catctttaac
     9121 aagggctcta ctcgttcgaa agccactttt tgctcaatac cgtaagccat agcaaccgtt
     9181 tcgatatgct ctctgagggt caattcctca tacagactag gcgtctcagg aatgtagcca
     9241 atctgcttgc ggtagctagt cgcatctcct tgcagagtca ggccattgat attgatggag
     9301 ccactataag gtgccaacag accgataatc tcattgatcg tcgttgattt cccagcacca
     9361 ttgagaccaa tcaaaccgac caactgccca ctttcaacag taaaggacac atctttcaaa
     9421 acaggaacat gaacatagcc acctgtcagg tttttaattt ctaacatatt ttctccaaat
     9481 ctggtataat gtagctatat tatatcaaaa tttagtacag tagaggtaga ttttatgtca
     9541 gattgcattt tttgtaaaat catcgcaggg gaaattcctg cttcaaaagt atatgaagat
     9601 gagcaggtcc ttgcctttct tgatatctct caagtaacac taggacacac cttggtcgtg
     9661 ccaaaagaac actatcgcaa tcttttggag atggatgcta cgagcgccag ccaactcttt
     9721 gcccaagtac caaaagtagc tcaaaaagtc atgaaagtca ctaaggctgc tggtatgaat
     9781 atcatttcca actgtgaaga agtcgctggt caaacagttt ttcatactca cgttcacctt
     9841 gtgcctcgct acagtgctga cgatgacctc aagattgatt ttatcgccca cgaaccagac
     9901 tttgacaaac ttgctcaagt cgctgaaacc atcaaaaacg cttaaggagt tgctatgaaa
     9961 ttatccaacc tactgctatt tgcaggagct gcagccggaa gttatctggt tacaaaaaat
    10021 cgccaaacca tcacagatga agtcttgaat accactgacc gcgttcaagc tatcaaggac
    10081 gatgtggata ttatccaaaa cagcctgcaa atcattaacc agcaaaaaga acttatcaag
    10141 gaataccaag aagacttgac ttacaagttt aaggtcttgg aaaaggatat ccagactaga
    10201 ctagctgtga taaaagaaat gcagggaact gaagataagt aaaaagagcc ccccggctct
    10261 tttttaaaac gtaaacaagt tatagtgaat tgaatttaga atagcacacc atagtttcta
    10321 aagcattgtt agagattact ttaaaatcct cttatcaatt tgttcatatt ctatttcaat
    10381 ctattatata tatcccatca actatgcatc ataatttagg taactcatac tcaataaaaa
    10441 tcaaaaagca aactagaaag ctaggcacag actgctcaaa acaccgtttt aaggttgtgg
    10501 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
    10561 tcagtaacca tacccatggc aagccgacgc tgacgtggtt tgaagaaatt tttgaagagt
    10621 atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa cacctgaaac
    10681 agcttggttt cagctgtccg gaaactttaa gacttaggtt caaagtttag gtatggaact
    10741 tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt cttaaaaaat ctataaagta
    10801 aaaagagccc gacagctctt tttgtttata ctcaatgaaa atcaaaaagc aaactaggaa
    10861 actagccacg ggctgctcaa aacactgttt tgaggttgta gatacgactc acatatatac
    10921 ggcaagccga cgctgacgtg gtttgaagag attttcaaag agtattattc tccatcaaag
    10981 gcatctttaa tatgatcaaa gaagcctttt ttctttggat ttactttcaa gtcaccagca
    11041 gccgcgaatt ctttcaaggc tactttttgg cggtcgttca agcctgtcgg tgttacgaca
    11101 ttaacagtaa cgtattggtc accaactgca ccgccacgaa ggctcggtgc ccccttacta
    11161 cgtaggcgga atttcttacc agtctgagtt ccctctggaa taaccaattc aacatcaccg
    11221 tgaacagttg gaatatctac tgtatcacca agagccgctt ggacaaagtt gaggttgaga
    11281 ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc tagcttccac agaaactact
    11341 acatacaagt caccataagg tccaccgtta aagcctgctt caccttgacc agcgaggcga
    11401 atttgttgac ctgtttccac accagcaggg attttcacat gtacgctatg agcttgtttc
    11461 tcatgacctg ttccatgaca ggttgtacat ggatatttga tttcttttcc ttggccgtga
    11521 cagacatcac aggttacttg gcgacgcatc ataccaagag gagtctgcgt atcgacgtta
    11581 atgacaccag cgccatgaca gcgtccacaa gtgactggac ttgtccctgg cttagcacca
    11641 gagccattac atgtacgaca gccagcttca cgatgatact taacttcctt ctcagttccg
    11701 aagatagctt cttcaaaggt caaattgaca cgatactgga gatcatctcc ttggcgagga
    11761 gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac ttgagaaaat atcctcaaaa
    11821 ccaccgaagc cacctgcccc attgaaaccg ccgaaaccac catctccacc aaaaccacca
    11881 ttggcgcctg cagcaccata ctggtcatag gcagcacgtt tttggtcgtc actcaaagtc
    11941 tcataggctt cttgaacttc cttgtacttg tcctcagcac caggctcctt gttgatatct
    12001 gggtgatatt ttttggaaag cttacgataa gcctttttga tttcgtctgc cgaagcgttt
    12061 ttggataccc ccagacgatc ataaaattca gtattgttca taattcaaga tacaaagggc
    12121 gtcaaacgga cacagccatc taggagtttt gacgacggac gcttatgtcc tagaaaaaac
    12181 tatctttttg gcacagtccg tagcccgtgt tcagttacga acacccttat tcctttctat
    12241 tgatgttctg aatcgaacca ccatttaggt cggattcaat tcctttcttt tatatttagt
    12301 aagaaattct gaaacccgtg ttcagttacg aacactcttt gttcctttct attgatgaga
    12361 caaaacccaa acaaatgaga ttccgtccct ttttatcgaa aaacagaggc tgggttgcaa
    12421 cctctgcaaa taagtaaatt aactttattt tgattttaaa tatcctataa gtacagaaat
    12481 atttatatat ttttgtctct tcttagtatt tgcctatata attccaattg atcataagaa
    12541 atacaactac tgtccaaatc ataccaatta tgaacggtat tacaaattta tatgtttgta
    12601 tttctaatga tatcatagct gataccatcg ccgatacaaa tgcagacaac aatactgaag
    12661 taacaaaatt ccttaccgct tcatcatttt tgacacaact atcatgctca gctaatataa
    12721 tttctctata tatagtcaaa cattctactg gaatattatc tataaaacct tttgctgctt
    12781 catagttgtc agcttgatta caaaaatcat tataaacttt tcgtgtattt tttagatact
    12841 catcatccaa tacactcatc ttatttttcc gtaaactctc cgtctacgac gtcatcgcct
    12901 gcgtttcctg ttgcttgtgc gccttctgct ccttcttgag cttgttgcgc tgctgcggct
    12961 tgttcgtaga gtttcacagc aaggccttga gctttttcgt tcaacgcttc aagtttcgct
    13021 ttcatgtcgt ccaagttgtt gtcttcttga gctttcttaa ggtcatcaag ggcagcttgg
    13081 gcagcgtcac gttctgcgtc gaagccttta ccttcagttt ccttgattgt cttttcagtc
    13141 gcaaagattg cttggtctac ttcattacga aggtctactt cttctttacg tttcttatca
    13201 gcttcagcgt ttgcttctgc atctttcatc atgcggtcga tttcttcgtc agtcaaacct
    13261 gagttcgatt ggatgacaat agtttgttct ttttgagttc caaggtcttt ggccttaaca
    13321 gacacgatac cgttcttgtc gatgtcaaat gttacttcga tttgaggaat tccacgaggt
    13381 gcagctggga tatcagtcaa ttggaagcgt ccaagagtct tgttatctgc tgccattggg
    13441 cgttcacctt gaagaacgtg gatatcaacg gctggttggt tgtctgctgc tgttgagaag
    13501 acttgtgatt tagatgttgg gattgtagtg ttgcgatcga taagttttgt aaatactcca
    13561 cccattgttt cgataccaag tgacaatggc gttacatcaa gaaggacaac gtccttgaca
    13621 tcaccagtaa tcacaccacc ttggatagcc gcacccatag caactacttc atcagggttt
    13681 actgatttgt ttggttcttt accagtttca gctttaacag cttcaacaac ggcagggata
    13741 cgagttgaac caccaacaag gataacttcg tcgatttctg acaagctcaa acctgcatct
    13801 gaaagggctt gacgaactgg aacttttgta cgttcaacaa ggtcacgagt caaatcgtca
    13861 aatttcgcac gagtcaaagt catttccaag tgaagaggtc cagcctcacc tgcagtgata
    13921 aatggcaagc tgatttgtgt tgaagttaca ccagaaaggt ctttcttcgc tttttcagcc
    13981 gcatctttca aacgttgcat tgccatcttg tcagtagaca agtcgatacc gttttctttc
    14041 ttgaattctg ctaccaagtg gtcaatgatt ttttggtcaa agtcgtcacc accaagtttg
    14101 ttgtcccctg cagttgacaa tacgtcgaag acaccgtcac ccaattcaag gatagagacg
    14161 tcgaatgtac caccaccaag gtcaaatacc aagatttttt cttctttgtc agtcttgtcc
    14221 aaaccataag caagagctgc tgcagttggt tcgttaacaa tacgttctac ttcaagacca
    14281 gcaattttac cagcgtcttt tgttgcttga cgttgagcgt cgttgaagta agccggaact
    14341 gtgataacag ctttggttac tttctcacca aggtagtctt cagcgtagcc tttcaagtat
    14401 tgaaggatca tagctgagat ttcttgtgga gtgtattctt ttccatttgc agaaactttt
    14461 tcagaagttc ccatcttaga tttgatagag ataactgtat ctgggtttgt aactgcttga
    14521 cgttttgcag catcaccaac gatgatttct ccgtttttga atgagactac agatggagtt
    14581 gtgcggtttc cttctgggtt tgcgatgatt ttgctttcag ttccttcaag aactgcaact
    14641 gctgagtttg ttgtacctaa gtcaataccg ataattttag acatgtgttt ttctccttgt
    14701 tagttaattt tctatttttc tttttgatac tcttcttaag tggcgacgcc gtcagagctt
    14761 gacttcgtca atctctttga ctaaactttg agcctaaggt ctcaaaattt gcgcaaatag
    14821 caccacggcg aagagtatcg tctttggttc gcttcgctca ctattacaaa gccaaacata
    14881 ttttatcttt cttcatagtt tattgtcgtt tcggacaagt tttctattat gtaaattgcg
    14941 acacgaggag tcgaaatcga ttttatttcg acgacgagtt agtaaggaag ctaggcaaac
    15001 gtcatagcga ttgccgtttt ctgacgagtt gctttagcta ccgtcagaat tgccgaatcg
    15061 aacacgccct aagctctgag taaaaaagat agattttctt gtgttcatcg aacacttcgt
    15121 caatctccta tttttactgc gagcttttta cgagctttgt atcttagtta tacaccacta
    15181 ccattgctgg gcgtaggatg cggtcatgga gtttgtagcc tttttgaaag acttgggcga
    15241 tggtatctac tgggtgttca tcgtctgctg ggagagtttg gatggccata tggtagttat
    15301 ggtcaaattc gccatctgct gcgatttctt caattccttc ttctttcaaa gcgtgaatca
    15361 agctttcttg caccatcgcc aagcccttct tcacatcatc tgtcaaacct tcaactgcaa
    15421 gtgcacgctc aaggttgtca agagatggta agattgcttt tgccaagtcc tggctacgat
    15481 aacgttgcaa gttttgacgt tcttcattgg cacggcgttg gatattttgc atttctgcat
    15541 gagcgcgaag atatttgttt tcgaactcat ctgcacgttc atttgccaag tccaactcag
    15601 acttttcagg agttgtttct tcagctgttt ccacaacttc ctcttcttga acttcttcta
    15661 cttcttcatt ttttatatct tgggccatgt tcgcctcctt taatgatttc aatcttaatg
    15721 tacttcgtaa tgattactgc tgaggtagcg gtaaaaatct gtcaacttca tggtcaaaac
    15781 acggttgacc acattgattt gattgattag ctgttggtaa tccagattaa ctggaccgat
    15841 aatggctaga attccaactc ctcgataagg aatgaggaac ttactactaa tcactgctag
    15901 gtcagctaaa caggactctt gaccgtctgc aacacgaaca ttttgcatct gatcctcacg
    15961 caacccctca cgaatctcca aggccacctt ttgcggttgg tcaaagaact gataggctgc
    16021 tagattggca aaattcaaga gatggacctt gcccgccatc acaatgtttt cgttgaacat
    16081 ttccttaaag atgtgttcaa agagatcgat gacattatcc gttgttgtaa agtaacgctg
    16141 gataatctgc ggaatctccg tccgaatctt gtagtgaata tctaaaacgg tgtgaccgag
    16201 gaaacgttcc tgaatgatgc tcttcagttt cagcaaatcc tcctgcaaga agttccttgg
    16261 aatcagaaac tgactagtaa ccgttcgcga ctcgtctagg gtaaataccg ccaaggctgt
    16321 atgttgcccc aaaacaacga tatcaaaggc tgtcaaacgt tgcctgctcg gctcaacatc
    16381 cagtgccact accgtacagc cactcaggtc tgttagtaag ttagcagcct cttgcagaat
    16441 atcctccaat ttgaagaatt cctgatcaaa ggctttgaca atctcatata cctcattttc
    16501 agccagccgg tcaaaatcca gtgagtgttt cacatagtac tgaaaaccag caacacttgg
    16561 catccgaccg cttgaagtat gagccttctc aagcaaccct tgtttttcta gttccgccat
    16621 gtcattacga atggttgcac tgctagagtt aatagactct tgcaaggctt ttgatccgac
    16681 aggttcgtgc gttttggtaa agatgtcaat aatcagattt aaaatatcct gctgacgctc
    16741 tgtaaccatc tcatcactcc tctctgtctg atcaattagc actcaatgca tccaagtgct
    16801 aactcatgat tctattatac acccttagaa gagaatgtca agacaaaaac tcaaaaaatt
    16861 agcactcttt tatcaagagt gctaaaaatc aatctgaaga cctagaacct tgctattcat
    16921 ctactcggta tttccttttt agtctgaaat aaccataaat tagataagaa atcagaaaag
    16981 catataaaac aaaaataaga aagaaagaca tagaaagatc ctctctaaac aaactcatac
    17041 aaacaacaag accacctgag aaagtagctg tacaggaacg aagtctagat tgcataactt
    17101 cccatttgag ccctttactc atacagactt gatcctctgg aagtaaatca gaagacgatt
    17161 tacgaagaat cgaacaagaa cctactccta tcaattccca acctctatct ctaaaatctt
    17221 gcagttcatg cttatacttt tttaagaaat ctagaatcat agatacggta gatgacatcg
    17281 tctggttgac attggtcaaa atagaacaaa ccaaaacgac tcgttctata cctccaacct
    17341 ttcaaatgca tctcatgtaa atgttcttct tccttgtcca aatcaacaat ggtgaaaatc
    17401 cgaaattcta ctctgctatt cattgtctta ccccaaaatt agaaaacatg cctggcgtta
    17461 tttattagat aattctttcc acttttgact caatctccaa aaaatataag aaatctgaat
    17521 cgcaaaaact atcaataaaa cccaatctat tatgaaaatc aaaaacactt tccaactgaa
    17581 agaactacct ccagtgacaa actttgagaa aaacggtagt agagctaaaa agagaaataa
    17641 aataggaagc atccgcattg ttaaaatccg tttggcataa aaaaatcttt atttaaacga
    17701 aaatattatg gcaaaattta cgccagtttt tgaacggctg atgtagatat tttatacttt
    17761 caaaatgttt aaatgtgatt atttattttt gaaaaataga tcaccagccc gactgaaagt
    17821 gcttatagaa tgataataag tcgcctgccg aaaacagcga aaaatagcgg tgttatgcgg
    17881 agataatctg acgcgatgcg aaagtatatt gcatacttat tttcaacaat ttagcagagt
    17941 atttttataa gtgtgatata atagaagtat aatttgttct gatagtttat tttatggaga
    18001 agtagatttt tagaatgcgg agggttcaat atggttgagt ttataaagtc taagaaagaa
    18061 atgagtgagg aggatattaa agcaaatttc atcactcctg ctattgtatc caaaggatgg
    18121 aaaaatggtg agcatatcgc ttacgaagaa tacttcactg atggtcgaat tgaagttaga
    18181 ggagataagg ctcatcgtaa agaaggaaaa aaatcagact attcactgta ttaccaattt
    18241 ggaactcgaa ttgcaattgt tgaggcaaag gataataaac acagcgttcg agcaggatta
    18301 caacaagcta ttgaatatgg agagatttta gatgttccat ttgtttattc ttcgaatggt
    18361 gatggcttta ttgaacacga ccgtatcacg agagaagaac gtgagctgga gttagacgaa
    18421 ttccctactc gtgaagaatt attttctcgt atgacgaagg aaaaaggatt gacgtacgaa
    18481 attacagaag ctatctcaac tccatactat acagacgcct tctcaatgaa aacgccacgc
    18541 tattatcagc aaatagctat caaccgtact attgaaacag ttgccagagg acaaaaacga
    18601 gtaatgtttg tgatggcaac aggaacgggg aaaacgttca tggcttttca aattattcat
    18661 cgccttcgaa aagctggttt ggctaaacga gttttattct tagcggatag aaacatctta
    18721 gtagaccaaa cgatggctga agactttagg ccattcgaaa aggtaatgac gaaaattaca
    18781 ccaaaacttt tgactgctcc tgaaaaatta aattcttttg aaatttatct agggctttat
    18841 cagcaactaa ctggtgaaga tggaactgaa acacattatc aaaaatttga caaagacttc
    18901 tttgatttaa tcgtaattga tgaagcgcac cgtggttcag ctaaggaaaa cagtaactgg
    18961 cgtaagataa ttgattattt cagttctgcg acacagattg ggatgaccgc tactcctaaa
    19021 gaaaccaaga atgcttccaa tacggaatac tttggtgagc caatctatac ttatagttta
    19081 aaacagggaa tcgaggatgg ttttttggct ccatatcgtg ttatgagagt taatttagat
    19141 gtagatgtgg atggttatcg tccagaaact ggaaaagttg atgctaacgg acaattaata
    19201 gaagataggt actacggcag gaaagatttt gataaaacca ttgtcattga tgatagaacg
    19261 caaagagttg ccaagtttgt ttctgattat atgaagcaaa acaatgcacg atttgataaa
    19321 acaattgttt tttgtgttga tattgaccat gccgagcgaa tgcgtgctgc acttgtaaaa
    19381 gagaatctag acttagtcca agaagactat cgttatgtca tgcaagtaac tggtgacaac
    19441 gctgaaggaa aagctcaact ggataacttt atggatgtca attctaattt tcccgctatt
    19501 gtaacaacgt ctaaattatt aacgacagga gttaatgcta aaacatgtcg tttgattgtt
    19561 ttagactcta atatccaatc catgactgaa tttaaacaaa ttattggtcg tggcacacgt
    19621 ctttatcctc aaaaggggaa agaatttttt acgattattg attttcgaaa tgttaccaat
    19681 ttgtttgctg accctgattt tgatggtgat ccagtgaagg tgctggaaac aggtgcgaaa
    19741 acagtcagtg gttctacgcc cggtttcgta agtgaggaag gtgacccagt agaaaaatat
    19801 atcgttacag acaagcaggt taccattctt aattctactg ttcaagtatt ggatgaaaac
    19861 gggaaactga ttaccgaaag cctgaccgac tacactcgga agaatatctt aggtagctac
    19921 gccactttga acgattttat cacagtttgg catacggcgg ataaaaagaa gcttatctta
    19981 gacgaacttt ataaaaaagg agtttatcta gatgctattc gagagtcgga gggaatatca
    20041 gaacaagaaa tcgatgattt tgatttactc ctaaaacttg cctatggtca aaaagaatta
    20101 accaaaacgg aacgtatcaa taagctcaaa caaagcggat atttatataa atatagtgag
    20161 gaagcgcgtg ctgttttgga aattttactg aacaaataca tggataaagg tattggagaa
    20221 ctcgaaagca ttgaaacatt aaaacttcca gaatttcaga tatatggtgg aaccttcaaa
    20281 atcatcaata cttattttgg agataaaaaa cgatatttac aagcaattaa agaattggag
    20341 caagagctat ttacagtagc ttaatgaaag gaaagtatgt caattacatc atttgtaaaa
    20401 agaattcaag atatcactcg aaacgatgct ggtgttaatg gtgatgctca acgtattgag
    20461 caaatgtctt ggttattatt cttaaaaatt tatgatagcc gtgaaatggt ttgggaatta
    20521 gaagaagacg agtatgagtc aattatccca gaggaattaa aatggcgaaa ttgggctcat
    20581 gctcaaaatg gggaacgggt attgacaggc gatgaattac ttgattttgt caataacaag
    20641 ttattcaaag agttgaaaga gcttgaaata acttcaaata tgcctattcg aaaaacgatt
    20701 gttaaatcag cttttgaaga tgcgaacaac tatatgaaaa atggcgtctt gttacgccaa
    20761 gtcatcaatg ctattgatga agttgatttc aatagccctg aagatcgtca ttcgtttaat
    20821 gatatttacg aaaaaattct taaagatatt caaaatgctg ggaactcagg agaattttat
    20881 acgccacgtg cagcgactga ttttattgcc gaagttcttg acccaaaact tggagaatca
    20941 atggcagacc ttgcttgcgg aacaggaggc ttcttgactt cgactctgaa ccgtttaagt
    21001 agtcaacgta aaactagtga agataccaaa aaatataata cagctgtttt tggtattgaa
    21061 aagaaagcat ttcctcatct tttagcagtt acaaatctgt ttcttcacga aattgatgac
    21121 cctaaaattg ttcatggaaa tactttggag aaaaatgttc gtgaatatac gaatgatgaa
    21181 aaatttgaca ttattatgat gaatccacct tttggagggt cagaattaga aacaataaaa
    21241 aataactttc cagcagaatt acggagttct gaaacagctg atttatttat ggctgtcatt
    21301 atgtatcgtt tgaaagaaaa tggtcgtgtt ggagttattt tacctgatgg ttttctattt
    21361 ggtgaaggtg taaaaactcg cttgaaacaa aaactggtag atgagttcaa cttgcatacg
    21421 attattaggt tgcctcatag tgtctttgca ccgtatacag gaatccatac gaacattctt
    21481 ttctttgata aaacaaagaa aacagaagaa acttggtttt atcgtttaga tatgccagat
    21541 ggttataaaa atttctcgaa aactaagccg atgaagtcag aacacttcaa tcctgttcgt
    21601 gactggtggg aaaatcgtga agagattctg gaaggtaagt tctacaaatc taaatcattt
    21661 acacctagtg aattggctga gttgaattat aatttagacc agtgtggttt tccaaaagag
    21721 gaagaggaaa tcttaaatcc ctttgagttg attcagaatt atcaagcgga aagagcaact
    21781 ttaaatcata agattgataa tgtattagct gatattcttc cgctcgatca gggattcaaa
    21841 aatcgacaag cacaaacaaa acagtcgcca tcatcattta ttgagataat atagtagatt
    21901 gaaataagat gtaaacaaat cgattaggaa agttaaatta gtttctagaa atttttagca
    21961 gatgtagtgt actattctag tctcaattta ctatggcttc aaatatatct ttcgaaaaaa
    22021 tatttacaga tgtgtaattt tgaagcttgt aaaagttagt aaacttgtag atttcgattt
    22081 gaagtaactt gttttcttgc ccgatattgt ttttgaaatt gaatttttcc atagtgactc
    22141 cttgattttc ttctacacgt ctgatgataa atctaattcg caaaagagtc aagaggattt
    22201 ttcgaaaaat aaatagcgac cgaaatcgct attttaaggg ttataggtat ttgatggctt
    22261 agactgctgt gtgactgttt acccacaggc aatctttctt ctatattagt attagtaaag
    22321 gtctaaataa ttatcaattt cccattgtga aacgaaggtt gcataacttg cccattcgat
    22381 tcgtttggct tcaaggaagc tagtgtagat gtgatctccg agagcagctt taaccacttc
    22441 atcttctgtc aaagctttca aagcgttgtg aagagttgat ggaaggtctg taataccagc
    22501 ttccttgcgc tcttctgctg tcatgatgta gatattttct tcgataggag ctggtgcttc
    22561 gattttattt tcaataccat acaaaccaac ttccaaaaga acagccatag caacgtaagg
    22621 gttcgccatt ggatccactg aacgcaactc aagacgagtt cccataccac gtgaagcagg
    22681 tacgcgcaca agtggcgaac ggttacgacc agcccaagca atgtaaacag gcgcttcata
    22741 acctggaacc aaacgtttgt atgagttaac tgttgggttc atgatggcag tatagttgta
    22801 agcatgcttg atcaaaccgc ctaggaaatg gtaagctgtt tctgacaact gcattccttt
    22861 tggatcattt ggatcaaaga aggcgttatt tccttctgca tcaaacaagg acatattaca
    22921 gtgcatacct gagccagcaa taccaaattt tggcttcgcc ataaatgttg cgtaaagtcc
    22981 gtgtttgcga gcaatggttt taacaacaag tttaaagatt tgaatcttat cacaagcacg
    23041 gagaacttca tcgtacttaa agtcaatctc atgctgtcca accgcaacct cgtggtgact
    23101 cgcttctact tcaaatccca ttttggtcaa gacattcaca atctcacgac gtgtgttgtc
    23161 cgcaaggtca gtaggtgcca aatcaaagta gccacccttg tcattcactt caagtgttgg
    23221 gtccccattt tcatccaact taaataggaa gaattctggc tctggaccaa ggttgaagga
    23281 tttgaatcca acttcttcca tgtgacgaag agctcgtttc aaattaccac gagggtcacc
    23341 cgcaaatggt tcaccttctg ttgtatagac atcacagatc agacctgcaa cacttccatt
    23401 ttcatctccc caagggaaga ctgtccatgt atccaagtcc gggtacaagt acatatccga
    23461 ctcattgata cgtacaaaac cttcaataga agatccatca aacataacct tgttcgataa
    23521 gaccttatct aactgttcat ctgtagcagg aatttcgacg tttttcatgg ttcccaaaat
    23581 atctgagaac ataagacgaa taaaggtaac atttttttcc ttgacttcac gacgaatatc
    23641 tgcagctgtg attggcataa gttttctcct taatctatga ctacttgcgg ttgcctaacc
    23701 gcgaccaaaa ggtgactgta ctgaagcaaa acgcccctgt tggaggagtt cattgtgaag
    23761 tgcacgacgt acttcagtct gactaaccgc tttcttggat ttcgcttcac gttcagcata
    23821 ttttttctta atggcagcga tattataacc ttcagagata taatctttga tttcaagcag
    23881 acgatccatg tcattcaagg aatacatgcg acgatttcct tcgtttcgat cgggcttgat
    23941 caactcttga tcttcataat aacgaatctg acgcgccgat aaatcggtca acttcataac
    24001 actgccgata ggaaaaacag ccatatttcg gcgaaattct ttttccttca tttacaattt
    24061 ccttctttct gtctattata gtctaaaaaa agacaaacgt caattgataa tgttataaaa
    24121 tgtaacatta tttttctttt ttctctaaaa agagacgaat acgatcaata tcgtaattta
    24181 caataattgc gacaaaaact cccataaacg tttctgatac acgcacaaac acgtacgaaa
    24241 ttgtctcacc acttggaatt gatagggtaa tgattaacat agctgctaca ccaccaataa
    24301 cccctgcttt gttattcatg gctacatttg tcataatggt taacatggtg cagattggaa
    24361 caactaccaa ggtcacccaa aaggcttcgt ggaaaaaggt atttaataag aagaagacca
    24421 aggcatagag tccaccgata ctatttccta gaatacgcga agtcccaaaa tgaacactct
    24481 tatcaaaact ctccctcagg ctaaaaacgg ctgtcaaagc accaatttga agacctttcc
    24541 agccaaaaaa gccaaaaatc aagagaacta gaaaaacagc aatacctgtt ttaaaggttc
    24601 gcataccaag tttgaactgg gatttatcga atttatattt tttaaaataa ctcataatct
    24661 caactttcta tttccatttt atcataaatc ggtgattttt atgagtaata gttgagagga
    24721 agcgttttta ttttaagcaa aagaaaagag gaactttcat ccctctcttc tttgatttgt
    24781 ttataaaatc ttatttttct gtcaaggctg caagtcctgg aagaacctta ccttcaagaa
    24841 gttccattga tgctccacca cccgtactaa tccatgagaa cttgtctgca cggccaaggt
    24901 taatcgctgc ggcagctgag tcaccaccac cgatgattga tttaactcct ggttgtttca
    24961 cgatagcgtc catcacaccg attgtaccag cttggaaatc tgggttttca aatacaccca
    25021 taggtccgtt ccatacaact gttttggcac cagtcaaagc ttcgtcaaat ttggcgatag
    25081 attttggacc gatgtcaaga ccaaggaagc cttcagaaac tgcttcacct tcagtgtcac
    25141 gcacttcagt gtaaccagca aatgcgttag cttcttttga gtcaactggc aagatcaatt
    25201 taccatttgc tttttcaaga agagctttcg caacatccaa tttgtcttct tctacaagtg
    25261 agttaccgat ttcgatacct tgtgctttgt agaatgtgta agtcatccca ccaccgataa
    25321 ggactttatc agctttttca agcaagtttt cgataacacc gatcttgtct gaaacttttg
    25381 aaccaccaag gatagccacg aatggacgtt ctggagtttc aactgcttct tggatgtagg
    25441 caatttcgtt ttcaagaagg aaaccagcaa ctgctttttc aacgtttgct gagataccaa
    25501 cgttagatgc gtgtgcacgg tgagctgtac cgaatgcatc gtttacgaag ataccatctc
    25561 caagtgatgc ccagtattta ccaagttcag gatcgttttt agattctttc ttgccgtcaa
    25621 catcttcgta acgagtgttt tcaaccaaga gaacttgtcc atcttcaaga gcgttgattg
    25681 ccgcttccaa ttcagcacca cgagtgacac ctgggaaaac aacatcttga ccaagttttg
    25741 ctgccaagtc tgctgctaca ggagcaagtg atttaccagc tttatcagct tcttctttca
    25801 cacgtccaag gtgagagaaa agaattgcac gtccaccttg ttcgatgatg tacttaatag
    25861 ttggaagagc tgctgtgata cggttatcgt tagtgattac gccatctttc aatggtacgt
    25921 tgaagtcaac acgaacgagg acttttttac ctttcaagtc aacgtcttta acagtaagtt
    25981 ttgccatgtt acaaaaactc ctttatttat tttattcgaa actattatat cacactttgg
    26041 aaatataagg aaagatttca caagattttt cgatatttaa tctttatctt ctcttttccg
    26101 tctcaaggtc aaggctaaac tagcaagacc gaggctggct aagcctgcta ttagggcttc
    26161 attagcatca gctgttcctg tatttggcaa ttgtttgctt gcttcttctg atttaactgc
    26221 tgtattttga gctggttttt cctgtgtagc tactactgct ggtactgttt ttacttttgt
    26281 tccaatttca acaatttctg gaatcgcttc ttggatgact tctgttgaac gaagacgacg
    26341 ctgaccgttt tcatcaactt caaagacatg tcttaacagt ccatctcgtc cctgtataat
    26401 gactcgttgt tccccaacta gcatctcagc attttcatgc tcttgacgat gaaaggcaac
    26461 cttttcctct tgaacttcca atttaggagc cttttgttga tcagaagccg gttgataagt
    26521 aggttttgga aggactttgt tttgtggtgc tggtttagca ggttgtggtt tttcagcaga
    26581 ttcataaaca aaggcataat gagtaaagtg aggtgctgta aagataacat gactatccgt
    26641 ttgttcaaaa gccaattcta ctgcctcttt gccttcaggt aagaaaaata ctttcttaac
    26701 tttcttatct ttttcaattg ggattttcac aatagaagca taagagagat caacatcttg
    26761 acctttttca tccaaacctt ctatttcaaa gacatgagca tcttctccag caaagtattt
    26821 cttctcttca gcacttgctt gaacacgctc tactttcaaa cccttgatga caggcttctc
    26881 tttatttgag aagtgtactt caacacctgt tttactatca gaaaagactg ctgacttgtc
    26941 ttctttcaat aaagcaacca gatcttttag actctgtttc gctgttgcaa gctcttctgg
    27001 actcaaatct actttttcaa ccaaggcctt gtttttctca agaagcggag ctagggcaga
    27061 acctgaagga tggtttggtt tggcagccaa gagttcttga gcctcacctg ttaagcgatc
    27121 aagctctgtc ttttcttccg taaatttctc ttgaccattc agagctttat gagcttctgt
    27181 caatttatta agtcggtcat tgacttgatc ttgggtgcta gtttcatggt ctttcaacac
    27241 ttgttcggcc gcttcaattg ctttcaagaa ggcttctttt acttctggtg aagcaaagtt
    27301 atagaccacc ttgttcttag cagcttcaac ttctgctaat ttttgtttga ggtactcatc
    27361 gttcaatgct gcttttggag ataccaaaac atcaaagttg acttcatgac cttggtaacg
    27421 aagcgtcaag gtttgacgac cagtcttttg agcatcgtaa ccagaaattt caactccctc
    27481 atcagtaaag gaatgttctt ccatggtgtc attgctataa gccactgcaa agcgtccttc
    27541 agacaagtct aagttatcac caactaggta atcttttttc ggttcctgac ttacttcaat
    27601 tcccaaaata gttttcggac ttgcttcatc ttggccagtg acagtcactg acaaattagc
    27661 atttactggt tgtcccaaat attggagagt aagattctgt tctcccttat gatgcgtatc
    27721 aaaacctgat actgatacac ctgcgtgagt taggcgaatg agttcgtcct cagttcctcc
    27781 ttcatactga actcgaagaa caccacctct aaggtccaaa tcctcccctt cggcgtagct
    27841 tgttttctta ggtcctgttt ctaggctgac tgacttgatt cttctgtcat cttttggaac
    27901 ggaaactgct aggacattac taatttcctt gcctggcaac tgggtacgat aataaagaag
    27961 accagattga tttgtcaaat ccaatggtgc acttgctagg tctcctccaa cttcgctctt
    28021 caaggttgcg agcggagttt gagaatttgg attatcataa acggtaatag ttgctccagc
    28081 cggtacatct gcaaagccaa cttgtacctt gttattgcct agagaacggg ctgcagcctt
    28141 ggccatcgga atattgacac tctcagtatc aagcttttca tacattttcc agttatagat
    28201 acgaatagcc ttccatggag ttccattgtc agaagtgaca acattcaagc gccagtcttg
    28261 agcagtgatt ggtttatcaa gagtgatatc tgtcacgtgt gctttgttac cacggacttc
    28321 cttagctagc ttccactcac catctgcatc tttataataa aggtcaaagt ctttagtgtt
    28381 catcaagcca tcgttaacag actcaccacc agctcctgca tgatccatga cccatctaac
    28441 aacagtacgt ggcttggtca aacgaatatc cacactacca ctcaactgag ctgaagacca
    28501 tttatctgac aagctagtaa tggtaccgtt caacatacct tcaatacctt ctccaccttc
    28561 agtcttaggg aaagtactat caataactgt tgcacctgga acgatatttt cagctagtgg
    28621 ttttggtagg ctggtatctt ttacagtcat accccaatca aaggttgtgg ttgcagcttc
    28681 tgaacgaact ccatttttac cgactgctac aaccttcagt tcttgagttg taccctgagc
    28741 acttgctgag cggctaactt ttggtagata aatagttgta gaagatgagc cagttagtaa
    28801 tttccagctg tctccatctt tttcataaac ttcatagaaa tctgcatcct tgttgccttt
    28861 aaattgcaca actgcttccg cttcttgggc atttttaaga gattgtttca ctacagaaaa
    28921 gcttgtcggc gattgtggct cttggtgatt gtccgagata gttaattgtc cgaggttgaa
    28981 ctgataatct tttacagcac cttcatgctc aaaaaatagt ttgactgcat agatggtttt
    29041 acctgctagt gagctgagag caaattcttc atttgtccag ttgtcagaaa gggttagctc
    29101 tttccatgca tctgcatcat cgaatttgta gtctggagtt gtagagaatg ccatataaac
    29161 tttagaacct tttcctccct tgtgggcaac acgaagtttg gtcttctcag ttacttctaa
    29221 cttagtagaa taaagtctca catcctgatc tgtcttaccg gctacatcac cagagaattt
    29281 aagggaattt ccgccattat aggcatctgt aaaatcatat tctgcacgaa gtttttcccc
    29341 tgttgaagtc tgccaccagc gccatgttgg aagaacacct gatactgaac gataattcca
    29401 ctcagaatcc ttagaaacct taccatctac gaaccatttt ttaccatgac ctgtattaaa
    29461 agaagtagta aaagtattac ctaccgctgg cgtacggtcc gcaactaggt tagcaatacc
    29521 ataccaatct ttgtcacctg gtttttggcc agtagggtct ccttgataac ctgtaaagaa
    29581 gatatcttca tttttatgat aatcttcacc agtttttcct aaacttgtaa tggtatctgg
    29641 ggcaaataaa ccaagagaaa ggcgcaattt cccattttcg tctaaaatgt cattccactt
    29701 aacctttgtc ttgtaggaac caccctgttg caattccaaa cctgcaaata catcataagg
    29761 attacgacca atccagttgg cagttgcaat agtgtaatca tttttagcct tatcccagtt
    29821 aaagttagca aagaagttat ctgccggaac cttatctcct tctggttgca tgaattggta
    29881 gttgtattct cccaaaccat cttgatgata acgtccatag ttataggtca tggcatcgta
    29941 ccaagaatac ttgattggat ggtttacctt agcagcatat tccttgctat agagcataaa
    30001 ctggcgcatc ttttctccaa gaggtttaac caaatctcca gttgtttctt ggttgatgaa
    30061 atagccatca tagccataat acttggccat gtctaccaat ttacgggcaa ttgggaagct
    30121 accatctgcg tcttgcttca aagcttcagc aaatctttct tgatctgcaa tactattaga
    30181 ccagttgaag aagagtgtac cgtatacagg aaccccgtta cggtgacctg catcaataac
    30241 gtcaggagtt ggtacgagac cttcccagaa gaccattgaa tctagatatt gccaatagtc
    30301 aaaagcatag gccttgaact cttctccacc aacagaagca tggtcttttg ctttagaatt
    30361 ggtgtttgat aaggcttgaa cttttgcttc cttgctagct ttttcattga ctaaatgacc
    30421 tgtccgacgt gaagcgagga caacagatcc gcggttaata gcatcatctt cacgagcacc
    30481 tggttcccat tttaacaatt cttcccaaga attaaatttg atttcctttg gttttaatgt
    30541 tttttctgta tttttaggga catctggctg gacttctttt tcagtcttat tagcttctgc
    30601 tgctggttta gactcatccc cttggttagt tgcttcgggc tttgcttctg tttccttgtc
    30661 agacttaggt tttgcttctg cagatggtgt ttctgcaacc acctcacttg ctttttcctc
    30721 tggtttagct gcgctagctg cttctgcagt ttcagatgct atcgctactc cttctgcctt
    30781 ttctagcttg ttttctctag catgttcccc ttcattctgc ttatcagctt cattggtcga
    30841 atgctcactc tctcctgaaa caagctctgg attagctccg ctattttcag gaactgcagt
    30901 ttcttcagcc aaggctggac cagcaaataa aacagcacca atcatcagcg agcaggctcc
    30961 tactgataac ttacgaatac tgtaacgaca acgtctttca aaaaatggat tcttcatttt
    31021 ctcctcctaa aagattgatt ttgttgtttt caagaaagcg ctttatattc tgtagttttt
    31081 tatcaaactc tagaaccatg ataatcacat ttttatccta aaacattttt agacaaaaaa
    31141 caataactgt ttgcacaaat ctaaaataag gttaaaaact cttaaaaata atacacttat
    31201 caattataat tttcttttac aaacttgaca ttaacacttg ttagatttac ttttatcctt
    31261 taaaatagaa taggagatat tccgcgatta tttttcggag gaaaaagaaa tgtccattaa
    31321 ttggcaggaa attttatttc actttttagg tggtctgggg ctattcttat atagcatcaa
    31381 gaccatggga gacggtttgc aacaagctgc tggagatcgc cttcgttttt acattgacaa
    31441 atatactagt aatcctttct ttggagttct ggttggtatt gggatgactg ctctaattca
    31501 gtctagttct ggtgtaacag ttatcacagt cggcctggtc agtgccggtc tcttaacctt
    31561 acgtcaggct atcgggattg tcatgggtgc taatattggg acaactgtca catcctttct
    31621 catcggtttt aaattaggta actatgccct acctatgctc tttatcggtg ccgtctgtct
    31681 cttttttaca aaaaatcgga cagtcaataa tatcggacgc atcctctttg gtttcggtgg
    31741 tatctttttt gccctcaatc tcatgagcgg cgcaatggct ccactcaagg atttacaggt
    31801 ctttaaggac tatatgattg agctaagtaa gaatcctgtt ttgggtgtct ttgtcggtac
    31861 cggcttgacc ttgctaattc aagcttcttc ggctaccatt gggattttac aaaacctcta
    31921 cgccggcaat ctaattgacc tacagggagc tttgccagtt ctatttggtg acaatatcgg
    31981 gacaaccatt acagccatca ttgcctcttt aggggctaat attgcagcta aacgggtagc
    32041 aggagctcat gttgccttca acgttatcgg aacagtcgtc tgcgttattt ttctagttcc
    32101 ttttactgtc ctgattcatt ggtttgaagc tacgctaaat ctagcaccgg aaatgaccat
    32161 cgcctttgct cacggaacct ttaatattac caacaccatt gtccaatttc catttatcgg
    32221 agctctggct tactttgtaa ccaagattat tcctggagag gacgaggttg tcaaatacga
    32281 acccttatat cttgatgaac atttcatcaa acaggcccca tctatcgctc taggaaatgc
    32341 taagaaagag ctcttgcact taggaaacta cgctgctaaa gcctttgacc tttcctataa
    32401 gtacatcatt gatttggatg aaaaagttgc tgaaaaaggg cataaaaccg aagaagcaat
    32461 taacaccatc gatgagcaat taacacgtta tctcattgcc ctttcaagcg aagctctcag
    32521 ccaaaaagaa agtgaagtgc ttaccaatat ccttgattcc tcccgtgatt tggaacggat
    32581 tggagaccac acggaggctc tactcaatct gactgactat cttcaacgga aaaatgttga
    32641 attttctgat gccgccttga aagaattaga ggaagtttac cgccaaacta gtgactttat
    32701 caaagatgct ctggatagtg tggaaaacaa tgatattgaa aaagcacgca gtcttgtaga
    32761 acgtcatgaa gcaatcaata agatagaacg tgttctcaga aaaacccaca tcaaacgcct
    32821 caacaaaggc gaatgttcaa cacaagctgg ggtcaacttt atcgacatca tctcacacta
    32881 cactcgtgta tcagaccacg ctatgaacct tgctgaaaag gtttttgcag aacaaatcta
    32941 agaaccaaga agctatccat cataattgga tggcttttta ctttttccta agcaagacta
    33001 ggctgaatga aactgaaaga gtattctgca gatatatagt atattgaaac tagaatagta
    33061 cacctcta
//
LOCUS       DBERCA010000017        32908 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.017, whole genome shotgun sequence.
ACCESSION   DBERCA010000017 DBERCA010000000
VERSION     DBERCA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.017"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            163..951
                     /gene="codY"
                     /locus_tag="ACL719_000932"
     CDS             163..951
                     /gene="codY"
                     /locus_tag="ACL719_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGS0698927.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            951..1526
                     /locus_tag="ACL719_000933"
     CDS             951..1526
                     /locus_tag="ACL719_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS0698928.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1608..1693
                     /locus_tag="ACL719_000934"
     tRNA            1608..1693
                     /locus_tag="ACL719_000934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Leu,seq:aag)
     gene            1966..3096
                     /locus_tag="ACL719_000935"
     CDS             1966..3096
                     /locus_tag="ACL719_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0698929.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            3597..4355
                     /locus_tag="ACL719_000936"
     CDS             3597..4355
                     /locus_tag="ACL719_000936"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0698930.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMRLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4442..4954
                     /locus_tag="ACL719_000937"
     CDS             4442..4954
                     /locus_tag="ACL719_000937"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGS0698931.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5136..6080
                     /gene="metA"
                     /locus_tag="ACL719_000938"
     CDS             5136..6080
                     /gene="metA"
                     /locus_tag="ACL719_000938"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGS0698932.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            6089..6766
                     /locus_tag="ACL719_000939"
     CDS             6089..6766
                     /locus_tag="ACL719_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS0698933.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            6864..7622
                     /gene="tpiA"
                     /locus_tag="ACL719_000940"
     CDS             6864..7622
                     /gene="tpiA"
                     /locus_tag="ACL719_000940"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGS0698934.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVVSKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7660..9165
                     /gene="lytC"
                     /locus_tag="ACL719_000941"
     CDS             7660..9165
                     /gene="lytC"
                     /locus_tag="ACL719_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGS0698935.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9661..10179
                     /locus_tag="ACL719_000942"
     CDS             9661..10179
                     /locus_tag="ACL719_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGS0698936.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10309..10815
                     /locus_tag="ACL719_000943"
     CDS             10309..10815
                     /locus_tag="ACL719_000943"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGS0698937.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10815..10985
                     /locus_tag="ACL719_000944"
     CDS             10815..10985
                     /locus_tag="ACL719_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698938.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            11017..12249
                     /gene="clpX"
                     /locus_tag="ACL719_000945"
     CDS             11017..12249
                     /gene="clpX"
                     /locus_tag="ACL719_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGS0698939.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKNENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12258..12845
                     /gene="yihA"
                     /locus_tag="ACL719_000946"
     CDS             12258..12845
                     /gene="yihA"
                     /locus_tag="ACL719_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGS0698940.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12856..13236
                     /locus_tag="ACL719_000947"
     CDS             12856..13236
                     /locus_tag="ACL719_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGS0698941.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13288..14178
                     /gene="rapZ"
                     /locus_tag="ACL719_000948"
     CDS             13288..14178
                     /gene="rapZ"
                     /locus_tag="ACL719_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGS0698942.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14175..15152
                     /locus_tag="ACL719_000949"
     CDS             14175..15152
                     /locus_tag="ACL719_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGS0698943.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQAILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15149..16060
                     /gene="whiA"
                     /locus_tag="ACL719_000950"
     CDS             15149..16060
                     /gene="whiA"
                     /locus_tag="ACL719_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGS0698944.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16094..17059)
                     /locus_tag="ACL719_000951"
     CDS             complement(16094..17059)
                     /locus_tag="ACL719_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS0698945.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17195..18052
                     /gene="cdaA"
                     /locus_tag="ACL719_000952"
     CDS             17195..18052
                     /gene="cdaA"
                     /locus_tag="ACL719_000952"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGS0698946.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            18039..18818
                     /locus_tag="ACL719_000953"
     CDS             18039..18818
                     /locus_tag="ACL719_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGS0698947.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18842..20194
                     /gene="glmM"
                     /locus_tag="ACL719_000954"
     CDS             18842..20194
                     /gene="glmM"
                     /locus_tag="ACL719_000954"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGS0698948.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KTIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTIEEVDYYVDTITDVVRAEIGID
                     "
     gene            20268..20642
                     /locus_tag="ACL719_000955"
     CDS             20268..20642
                     /locus_tag="ACL719_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGS0698949.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20644..21492
                     /locus_tag="ACL719_000956"
     CDS             20644..21492
                     /locus_tag="ACL719_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0698950.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21758..22525
                     /gene="dapB"
                     /locus_tag="ACL719_000957"
     CDS             21758..22525
                     /gene="dapB"
                     /locus_tag="ACL719_000957"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGS0698951.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22522..23721
                     /locus_tag="ACL719_000958"
     CDS             22522..23721
                     /locus_tag="ACL719_000958"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGS0698952.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAI
                     TEKYQSLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            23718..25589
                     /locus_tag="ACL719_000959"
     CDS             23718..25589
                     /locus_tag="ACL719_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0698953.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25732..26916)
                     /gene="mntE"
                     /locus_tag="ACL719_000960"
     CDS             complement(25732..26916)
                     /gene="mntE"
                     /locus_tag="ACL719_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGS0698954.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27220..29916
                     /locus_tag="ACL719_000961"
     CDS             27220..29916
                     /locus_tag="ACL719_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS0698955.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVILANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29929..30720
                     /gene="yghU"
                     /locus_tag="ACL719_000962"
     CDS             29929..30720
                     /gene="yghU"
                     /locus_tag="ACL719_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGS0698956.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30836..31246
                     /locus_tag="ACL719_000963"
     CDS             30836..31246
                     /locus_tag="ACL719_000963"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS0698957.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31258..32853
                     /locus_tag="ACL719_000964"
     CDS             31258..32853
                     /locus_tag="ACL719_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698958.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRVYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT         9704 a         6107 c         7584 g         9513 t
ORIGIN      
        1 aaaataggca acttgtcaat agaaaaggaa caatttaatt aaaaatattc tggtttttaa
       61 ttaaattgta agtcaatgca agtcaataag gaattttcag ataattattg aaaaagagac
      121 gtttttatgt tataatgata gcgattaaat tcgaggtgaa aaatggcaca tttattagaa
      181 aaaactagaa aaattacatc aattttgaaa cgctcagagg agcagttgca ggatgagctc
      241 ccttacaacg ctattacgcg tcagttagcg gatattattc attgcaatgc ctgcattatc
      301 aatagtaagg gacgtctgct tggctatttt atgcgttata aaacaaatac agatcgcgta
      361 gagcaattct tccaaactaa gattttccca gatgactacg ttcaaggggc taatatgatt
      421 tacgaaacag aagcaaactt gcctgttgag catgatatga gtattttccc tgttgagagt
      481 agagatgatt ttccagatgg cttgacgact attgcaccga ttcatgtatc ggggattcgc
      541 cttggttctt tgattatttg gcgtaatgat aaaaaattcg aagatgagga cttggttctt
      601 gttgagattg ccagtaccgt tgttgggatt cagcttctta acttccaacg tgaagaagat
      661 gagaaaaata ttcgtcgtcg tactgctgtc accatggcgg tcaataccct ttcttactcc
      721 gaactccgtg ctgtttcagc aattttaggg gaattaaatg gaaatgaagg gaagttgact
      781 gcgtcagtga ttgcagatcg tatcggaatc actcgctctg tgattgtcaa tgctcttcgt
      841 aaacttgagt ctgcggggat tattgaaagt cgctcacttg gaatgaaggg aacctatctt
      901 aaggtcttga tttcagatat ttttgaagaa gtgaagaaaa gagattacta atgacaaagg
      961 ctttaatttc gattgattat acagaagatt ttgttgctga tagtgggaag ctgacagcag
     1021 gtgctccagc tcaggcgatt tcggatgcca tcagcaaggt aactcgatta gcttttgaac
     1081 gaggagatta tatcttcttt actattgatg ctcatgaaga aaacgatttt ttccatccgg
     1141 aaagtaagtt atttcctcct cataatctga ttgggacgag tggacggaat ttatatggag
     1201 atttggggat cttttatcaa gagcatggtt cagacagtcg tgtcttttgg atggataaac
     1261 gccattactc agctttttca gggactgacc tagatattcg tttgagagag cgtagagtgt
     1321 ctaccgttat cttaacaggt gtcttgacgg atatctgtgt cctacataca gctatagatg
     1381 cctataatct aggatatgac atcgagattg ttaaaccagc tgttgcttcc atctggcctg
     1441 aaaatcatca atttgcccta ggtcatttca aaaatacact cggagctaag ttagtagatg
     1501 aaaatctaaa tgaactttct gagtaacctg gttggtgaaa atgaaaaaat ttgaaaaaaa
     1561 gtgttgacaa gcatcccaaa agttgatata ctagtaaagt aatcgatgcg gggatggcgg
     1621 aattggcaga cgcgcaggac taaggatcct gtgaccgctt taggtcgtga gggttcaagt
     1681 ccctctctcc gcataggaaa agagttagct aaagctagct cttttgtatt tctctaaaaa
     1741 attagaatcc agtcaaatgt agtggagaat cagcgaaaat atgggaaaac tgatccttgt
     1801 ttctgctttt gtcatagaag aataataaat tcaaattctc cgatattctc taaagttttg
     1861 tgcaagttgc acagaacttt tttatttttt tggtcatctt gccatagaaa tataaagcgt
     1921 tttcatctat aatataatta tcaaaagaca aaaggagttc acctcatggt agaattgaat
     1981 cttaaaaata tttacaaaaa atatccaaac agcgaacact attcagttga agatttcaac
     2041 ttgaacatca aagataaaga atttatcgtt ttcgtaggac cttcaggatg tggtaaatca
     2101 actacactcc gtatgattgc tggtcttgaa gacattacag aaggtactgc atctatcgat
     2161 ggcgtggttg tcaacgacgt agctccaaaa gaccgtgata tcgccatggt attccaaaac
     2221 tacgctcttt acccacacat gactgtttat gacaacatgg ctttcggttt gaaattgcgt
     2281 aaatacagca aagaagacat taacaaacgt gttcaagaag cagctgaaat acttggattg
     2341 aaagaattct tggaacgtaa accagctgac ctttcaggtg gtcaacgtca acgtgttgcc
     2401 atggggcgtg cgattgtccg tgatgcgaaa gtattcttga tggacgaacc tttgtcaaac
     2461 ttggatgcca aacttcgtgt atcaatgcgt gctgaaatcg ctaaaattca ccgtcgtatc
     2521 ggagctacaa ctatctatgt aactcacgac caaacagaag cgatgacact tgcagaccgt
     2581 atcgttatta tgtcagctac taagaaccct gctggtacag gtactatcgg acgtgtagaa
     2641 caaatcggta ctcctcaaga agtttacaaa aatccagtta acaaatttgt tgcaggattc
     2701 atcggaagcc cagctatgaa tttcatcaac gtgaaattgg ttggtagcga aattgtttct
     2761 gacggtttcc gtttgaaagt gccagaagga gcattgaaag ttcttcgtga aaaaggctac
     2821 gaaggaaaag aattgatctt tggtatccgt ccagaagacg tgaatgcaga acctgctttc
     2881 cttgaaacat tcccagactg tgttgtaaaa gcgactatct ctgtatcaga actgcttggt
     2941 tcagaatctc acctttactg ccaagttggt aaagacgagt ttgttgcaaa agttgatgct
     3001 cgtgactact tgcaaacagg tgcaacagtt gagcttggat ttgacttgaa caaagcacac
     3061 ttcttcgatg tagaaactga aaaaacaatc tactaaaata aataaaattc aaagcactac
     3121 aagaaaagat atctctttat caattgtagt ggagagatat cagttaatct agggagagaa
     3181 acaaaatgct tctctccttt ttgctaggga agtcatatta tgcatctata ttgtgatgct
     3241 ctttaatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc gtacgtatga
     3301 ttactgattt cgtcagtttt atctgcaacc tcaaagatgt actttgagca gcttacggct
     3361 agtttcctag tttgctcttt gatttccatt gagtattatt tgtgggtacc atctacaagt
     3421 gaagctatat gcgtaaacta cgtgagcaat tgaattcgaa ctagagaggt aataataaat
     3481 ttatgctata gttatggtga cttgtatgct tttgattcta gtttatcaaa taatagatta
     3541 gaattgtcag ataatatcat tttgtgttat aatgaagaaa aaacagaggt gttcaaatgt
     3601 cagaagcagg tcataagttt ttagcaaaat tggggaaaaa acgcttacgt ccaggtggaa
     3661 agcgtgccac agattggtta attgcagaag gaggattttc aaaagaaaag agaatactag
     3721 aggttgcgtg taatagggga actacagcaa ttgagttggc acagcgtttt ggttgtaaga
     3781 taactgctgt tgatatggat gctcaagctt tagaagtggc taaaaaatct gctggaacgg
     3841 caggtgttgc tcatttaatc agttttgaaa gagcaaatgc aatgaaactt ccttatcaag
     3901 atgctagttt tgatattgtt ataaatgaag ctatgctgac tatgcaagcc gatcaagcta
     3961 agaaaaaatg tgtaatggaa tatctaaggg tattaaaacc tggaggtctt ctattgacgc
     4021 atgatgtgct tcttaaggaa gctaaagagt ctatcagaca ggaattatca caagcaattc
     4081 atgtaaatgt aggtccttta actcaagatg gttgggaaca ggtgatgata gaatcaggtt
     4141 attgtgatgt gaaagtattg actggtgaaa tgacattaat gagattatcg ggtatgattt
     4201 atgacgaagg tttgctagga actttgaaaa tttgtgtaaa tgcttgtaaa aaggagaata
     4261 gaaagcagtt tttaactatg tataaaatgt ttgctaagaa taaacagaaa ttgggcttta
     4321 ttgcgatggc tagttataaa tcgtcaaaac gttagataat tattgaagtt aacttttcct
     4381 tttttctttc ttaaaaaata tgctataata gagagtaaaa aactttgaaa gaaagaaaaa
     4441 gatgaattta aaagattaca ttgcaacaat tgaaaattat ccaaaggaag gcattacctt
     4501 ccgtgatatt agtcctttga tggctgatgg aaatgcttat agctacgctg ttcgtgaaat
     4561 cgttcagtat gctactgaca agaaagtcga catgatcgtg ggacctgaag ctcgtggatt
     4621 tatcgtgggt tgtccagttg cctttgagtt gggaattggt tttgcgcctg ttcgtaagcc
     4681 aggtaaattg ccacgcgaag ttatttctgc tgactatgaa aaagagtacg gtgtcgatac
     4741 cttgactatg cacgcggatg ccattaagcc aggtcaacgt gttcttattg tagatgacct
     4801 tttggcgaca ggtggaactg ttaaggcaac tatcgagatg attgaaaaac ttggtggtgt
     4861 tatggcaggt tgtgccttcc ttgttgaatt ggatgaattg aacggccgtg aaaaaattgg
     4921 tgactacgac tacaaagttc ttatgcatta ttaatgaaaa cagtccctag ggctgttttc
     4981 tctacactag gatataaaaa tagactataa ctagttagag aaaaactata attgaaaact
     5041 atatcttctt gcagtataat aaaaggacta agtgtttgag atttgtcttc aaacatatgc
     5101 aattattcct gaaagagtac agttaggaga gggttatgcc gattcgaatt gataaaaaat
     5161 tgccagctgt tgagatttta cggacagaga atatctttgt catggatgat caacgtgctg
     5221 cccaccaaga tatccgtcct ttgaagattt taattttaaa tctcatgcca cagaaaatgg
     5281 tcacagagac ccagttgttg cgccacttgg ctaatacacc cctacaactg gatattgatt
     5341 ttctctatat ggagagccac cgttctaaaa caactcgttc agagcacatg gagaccttct
     5401 ataaaacttt tcctgaagtc aaggatgagt attttgatgg gatgatcatc acgggtgctc
     5461 cagttgagca tttaccattt gaggaagtgg actattggga ggaatttaga cagatgcttg
     5521 agtggtctaa gactcatgtc tattcgaccc ttcatatctg ttggggggct caggctgggc
     5581 tttatctgcg ctatggtgta gaaaaatacc agatggacag taagctatca ggtatttatc
     5641 ctcaggacac cctaaaagag ggtcaccttc tatttagagg ctttgatgat agctatgtat
     5701 cccctcattc acggcacacg gagatttcta aggaagaggt cttaaacaag accaatctcg
     5761 agattttatc agaaggacct caggttgggg tttctatttt ggccagtcgt gatttacgag
     5821 aaatttatag ttttggtcat ttggagtatg accgtgatac cttggcaaaa gagtattttc
     5881 gagatcgtga tgcaggtttc gacccacata ttccagaaaa ttactttaaa gatgatgatg
     5941 ttaatcaggt accttgtctt tgttggtctt catctgcagc cctctttttc agtaattggg
     6001 taaaccatgc ggtctatcag gagacgcctt ttgactggag aaagatagaa gatgatgcat
     6061 ctgcatatag gtatttataa gaggaattat gacatattta gacgctttta aatcaggtac
     6121 cttggtttta ccgagtgccc tgctcttgca ttttaaggaa ctctttcctt ctagcgacga
     6181 ttttctggtt tggcaatttt tctatttgca aaatacgaca ggcttagaag aaatatcgcc
     6241 aagccagatt gctgaaagga ttggcaagga aatttcggat gtcaaccagt ccatttctaa
     6301 tctgacggaa aggggactgc tccagtatcg tactatcgaa ttaaatggcg aaattgaatt
     6361 gctctttgat gctagtttgg ccttggaacg tttggatgac ctgtttggag cagttcattc
     6421 aagttcagac cagctaacac cacaaaacca gctcaaggat ttggtggaaa ccttccagca
     6481 ggagttggga cgattgttga cgccttttga gattgaggat ttgaccaaga cactaaagga
     6541 agatggaacc agtgctgact tgattaagga ggctcttcgt gaagctgttt tgaacggaaa
     6601 accaaactgg aagtacattc aggcgatttt gagaaactgg cgccatgaag gaatcaagag
     6661 tgtggctcaa attgaggcca agagagcaga aagagaagca agcaatcctc agttgacaca
     6721 ggtatctgca gatttcataa atgccatgga tctctggaag gattaatcca tgcaagtagg
     6781 cttgaaatcc gagtaagatt tgcaagctgt gtataattgt gatagaataa atagaaaata
     6841 aattgaaaaa agaggtatgt gaaatgtcac gtaaaccatt tatcgctggt aactggaaaa
     6901 tgaacaaaaa tccagaagaa gctaaagcat tcgttgaagc agttgtatca aaacttcctt
     6961 catcagatct tgttgaagca ggtatcgctg ctccagctct tgatttgaca actgttcttg
     7021 ctgttgcaaa aggctcaaac cttaaagttg ctgctcaaaa ctgctacttt gaaaatgcag
     7081 gtgctttcac tggtgaaact agcccacaag ttttgaaaga aatcggtact gactacgttg
     7141 ttatcggtca ctcagaacgc cgtgactact tccatgaaac tgacgaagat atcaacaaaa
     7201 aagcaaaagc aatctttgcg aacggtatgc ttccaatcat ctgttgtggt gaatcacttg
     7261 aaacttacga agctggtaaa gctgctgaat tcgtaggtgc tcaagtatct gctgcattgg
     7321 ctggattgac tgctgaacaa gttgctgcct cagttatcgc ttatgagcca atctgggcta
     7381 tcggtactgg taaatcagct tcacaagacg atgcacaaaa aatgtgtaaa gttgttcgtg
     7441 acgttgtagc tgctgacttt ggtcaagaag tcgcagacaa agttcgtgtt caatacggtg
     7501 gttctgttaa acctgaaaat gttgcttcat acatggcttg cccagacgtt gacggtgccc
     7561 ttgtaggtgg tgcgtcactt gaagctgaaa gcttcttggc tttgcttgac tttgtaaaat
     7621 aatcagtaag tagcaaaagc taggtggaac agcattcaga tgtctgttac attttttata
     7681 ggagaggaaa gattgaaaat aaaaattgga ttagcaagta tctgtttact aggcttggca
     7741 actagtcatg tcgctgcaaa tgaaactgaa gtagcaaaaa cttcgcagga tacaacgaca
     7801 gcttcaagta gttcagagca aaatcagtct tctaataaaa cgcaaacgag cgcagaagta
     7861 cagactaatg ctgctgccca ctgggatggg gattattatg taaaggatga tggttctaaa
     7921 gctcaaagtg aatggatttt tgacaactac tataaggctt ggttttatat taattcagat
     7981 ggtcgttact cgcagaatga atggcatgga aattactacc tgaaatcagg tggatatatg
     8041 gcccaaaacg agtggatcta tgacagtaat tacaagagtt ggttttatct caagtcagat
     8101 ggggcttatg ctcatcaaga atggcaattg attggaaata agtggtacta cttcaagaag
     8161 tggggttaca tggctaaaag ccaatggcaa ggaagttatt tcttgaatgg tcaaggagct
     8221 atgatgcaaa atgaatggct ctatgatcca gcctattctg cttattttta tctaaaatcc
     8281 gatggaactt atgctaacca agagtggcaa aaagtgggcg gcaaatggta ctatttcaag
     8341 aagtggggct atatggctcg gaatgagtgg caaggcaact actatttgac tggaagtggt
     8401 gccatggcga ctgacgaagt gattatggat ggtactcgct atatctttgc ggcctctggt
     8461 gagctcaaag aaaagaaaga tttgaatgtc ggctgggttc acagagatgg taagcgctat
     8521 ttctttaata atagagaaga acaagtggga accgaacatg ctaagaaagt cattgatatt
     8581 agtgagcaca atggtcgtat caatgattgg aaaaaggtta ttgatgagaa cgaggtggat
     8641 ggtgtcattg ttcgtctagg ttatagcggt aaagaagaca aggaattggc gcataacatt
     8701 aaggagttaa accgtctggg aattccttat ggtgtctatc tctataccta tgctgaaaat
     8761 gagaccgatg ctgagaatga cgctaaacag accattgaac ttataaagaa atacaatatg
     8821 aacctgtctt accctatcta ttatgatgtt gagaactggg aatatgtaaa taagagcaag
     8881 agagctccaa gtgatacaga cacttgggtt aaaatcatca acaagtacat ggacacgatg
     8941 aagcaggcgg gttatcaaaa tgtgtatgtc tatagctatc gtagtttatt acagacgcgt
     9001 ttaaaacacc cagatatttt aaaacatgta aactgggtag cggcctatac gaatgcttta
     9061 gaatgggaaa acccttatta ttcaggagaa aaaggttggc aatatacctc ttctgaatac
     9121 atgaaaggaa tccaagggcg cgtagatgtc agcgtttggt attaagcgat gatttgaaag
     9181 agggatgtga tagtagcacc ctctttttct ttgttttatg atagttcatc ctcgagtaaa
     9241 ttcaagatct tgctcggaaa tgaagcttat atagtagatt gaatatagac aaataccttg
     9301 tgattggtaa aacattttag aaattcattt acctttccta atcgacttgg tttcatctta
     9361 tttcaatcta ttatagtatt ggggaatttc ttcaaaccac atcagcttgg tcagttctac
     9421 ctgcgacctc aaaacttgtg ctttggtcaa gctgggttta gtttcctagt ttgctgatgg
     9481 atttccattg actataagca tccaaccctc tttttgtctt ctaaagaatt cttaaattat
     9541 cagtctattg caacttttct catataagtt ctttgtcttg ctattggttt tccttagtag
     9601 tatactaagg tagtaatcat taagaagtgg ttacaaaaaa taatgaatga ggtaaagaaa
     9661 atggtagaat tgaaaaaaga agcagtaaaa gacgtaacat cattgacaaa agcagcgcca
     9721 gtagcattgg caaaaacaaa ggaagtcttg aaccaagctg ttgctgattt gtatgtagct
     9781 cacgttgctt tgcaccaagt gcactggtat atgcatggtc gtggtttcct tgtatggcat
     9841 ccaaaaatgg atgagtacat ggaagctctt gacggtcaat tggatgaaat cagcgaacgc
     9901 ttgattacac tcggtggaag cccattctct acattgacag agttccttca aaatagtgaa
     9961 atcgaagaag aagctggtga ataccgtaat gttgaagaaa gcttggaacg tgttcttgtt
    10021 atctaccgtt acttgtcaga acttttccaa aaaggtttgg atgtcactga tgaagaaggt
    10081 gacgatgtga caaacggtat ctttgcaggc gctaaaactg aaacagataa aacaatttgg
    10141 atgcttgcag ccgaacttgg acaagcacct ggtttgtaag aaataaggaa acatagataa
    10201 atctgtagga aatttcttac agattttttc tattttgtaa gctattccaa accagtcttt
    10261 ttttgagttt tttgataaaa tagtactatc agtgaaaagg atggaagcat gactaagaaa
    10321 atcgtagcta tttgggccca ggatgaagag ggtgtgattg gtaaggacaa tcgtctgcct
    10381 tggcatttgc cagcagaatt gcagcacttt aaagaaacaa ctctgaatca tgctatcttg
    10441 atggggcgtg tgacctttga tgggatgggg cgtcgcttgc ttccaaaacg ggaaaccctg
    10501 attttgacgc gtaatccgga agaaaagata gatggggttg ctacttttca ggacgtccag
    10561 tctgttcttg actggtatca ggctcaagaa aagaatctct acattatcgg tgggaagcaa
    10621 atttttcagg cttttgaacc ttaccttgat gaagtgattg tcactcacat tcatgctcgg
    10681 gtggaaggag atacctattt ccctgaagaa cttgacttgt ctctttttga gacagtttca
    10741 agcaaatttt acgccaaaga tgagaagaat ccttatgatt ttaccatcca ataccgcaag
    10801 agaaaggaag tctaatggaa cggagtttat ttggtctatt cacggctttt ctgtgtttca
    10861 tctgctttct ggcaggagca caggcttttc gtaaaaagcg ttacgggctt tctatcctcc
    10921 tttggttaaa tgcctttacc aatctggtca atagtattca tgctttttac atgaccttat
    10981 tttagataga atgacagtat agaatggaag gaaatcatgt ctacaaatag aaaaaatgat
    11041 atgatggttt attgctcatt ttgtggcaaa aaccaagaag aagtacaaaa aataattgct
    11101 ggcaacaatg cttttatttg taatgaatgc gtggagttag ctcaggaaat cattcgagaa
    11161 gaattggttg aggaagtctt ggcagacttg tctgaggtgc caaaaccaat tgaactcctc
    11221 catatcttga accactatgt aattggtcaa gatcgtgcca agcgtgcctt ggcagtggcg
    11281 gtttataacc actacaaacg catcaatttc cacgatacac gcgaagagtc agaagatgtg
    11341 gatttgcaga agtcaaacat tttgatgatt ggcccaactg gttcagggaa aactttcctt
    11401 gcccagacct tggctaagag cttgaatgta ccttttgcta ttgcggatgc gacagctctg
    11461 acggaggctg gttatgtggg tgaggatgtg gaaaatatac tcctcaaact cttgcaggtt
    11521 gctgacttta acatcgaacg tgcagagcgt ggcattatct atgtggatga aattgacaag
    11581 attgccaaga agaatgagaa tgtgtctatc acacgtgatg tttctggtga aggggtgcaa
    11641 caagcccttc tcaagattat cgagggaact gttgctagcg taccgcctca aggtggacgc
    11701 aaacatccac aacaagagat gattcaagtg gatacaaaaa atatcctctt catcgtgggt
    11761 ggtgcttttg atggtattga agaaattgtc aaacaacgtc tgggtgaaaa agtcatcgga
    11821 tttggtcaaa acaataaggc gattgacgaa aacagctcat acatgcaaga aatcatcgct
    11881 gaagacattc aaaaatttgg tattatccct gagttgattg gacgcttgcc tgtttttgcg
    11941 gctcttgagc aattgaccgt tgatgacttg gttcgcatct tgaaagagcc aagaaatgcc
    12001 ttggtgaaac aataccaaac cttgctttct tatgatgatg ttgagttgga atttgacgac
    12061 gaagcccttc aagagattgc taataaagca atcgaacgga agacaggggc gcgtggactt
    12121 cgctccatca tcgaagaaac catgctagat gttatgtttg aggtgccgag tcaggaaaat
    12181 gtgaaattgg ttcgcatcac taaagaaact gtcgatggaa cggataaacc gatcctagaa
    12241 acagcctaga ggtgactatg gaacttaata cacacaatgc tgaaatcttg ctcagtgcag
    12301 ctaataagtc ccactatccg caggatgaac tgccagagat tgccctagca gggcgttcaa
    12361 atgttggtaa atccagcttt atcaacacta tgttgaaccg taagaatctc gctcgtacat
    12421 caggaaaacc tggtaaaacc cagctcctga acttttttaa cattgatgac aagatgcgct
    12481 ttgtggatgt gcctggttat ggctatgctc gtgtttctaa aaaggaacgt gaaaagtggg
    12541 ggtgcatgat tgaggagtac ttaacgactc gggaaaatct ccgtgcggtt gtcagtctag
    12601 ttgaccttcg tcatgacccg tcagcagatg atgtgcagat gtacgaattt ctcaagtatt
    12661 atgagattcc agtcatcatt gtggcgacca aggcggacaa gattcctcgt ggtaaatgga
    12721 acaagcatga atcagcaatc aaaaagaaat taaactttga cccaagtgac gatttcatcc
    12781 tcttttcatc tgtcagcaag gcagggatgg atgaggcttg ggatgcaatc ttagaaaaat
    12841 tgtgaggaaa agaaaatggc aaaaacaatt catacagata aggccccaaa ggctatcggg
    12901 ccctatgttc aaggaaaaat cgttggcaac cttttgtttg ctagcggtca agttccccta
    12961 tcccctgaaa ctggggaaat tgtaggagag aatatccaag aacagacaga gcaagtcttg
    13021 aaaaacatcg gtgctatttt ggcagaagca ggaacagact ttgaccatgt tgtcaaaaca
    13081 acttgtttct tgagcgatat gaacgacttt gttcctttta atgaggttta ccaaacggcc
    13141 ttcaaagagg aattcccagc tcgttcagct gtggaggtag ctcgtcttcc tcgtgatgta
    13201 aaagtcgaaa ttgaagtcat cgcagagatt ggataagcta gttgaagttt ggtgttgcca
    13261 aacttctttt gatataagga gaaaaagatg acaaagaaac aacttcactt ggtgattgtg
    13321 acagggatga gtggcgcagg gaaaactgta gccattcagt ccttcgagga tctaggttat
    13381 ttcaccattg ataatatgcc gccagctctc ttgcctaagt ttttgcagct ggttgaaatt
    13441 aaggaagaca atcctaagtt ggccttggta gtggatatgc gtagccgttc tttcttttca
    13501 gagattcaag ctgttttgga tgagttggaa aatcaagatg gtttggattt caaaatcctc
    13561 tttttggatg cggctgataa ggaattggtc gctcgttaca aggaaaccag acggagtcac
    13621 ccactagcag cagacggtcg tattttagat ggaatcaagt tggaacgtga actcttggca
    13681 cctttgaaaa atatgagcca aaatgtggtg gatacgactg aactcactcc acgtgagctg
    13741 cgcaaaaccc ttgcagagca gttttcagac caagaacaag cccagtcttt ccgtatcgaa
    13801 gtcatgtctt tcggatttaa gtatggaatc ccgattgatg cggacttggt ctttgatgtc
    13861 cgtttcttgc caaatcccta ttatttacca gaactgagaa accaaacggg tgtggatgaa
    13921 cctgtttatg attatgtcat gaaccatcct gagtcagaag acttttatca acatttattg
    13981 gccttgattg agccgattct gccaagttac caaaaggaag gtaagtccgt tttgaccatt
    14041 gccatgggat gtacgggtgg acaacaccgt agtgtggcat ttgctaaacg cttggtgcag
    14101 gacttatcca agaattggtc tgttaatgaa gggcatcgcg acaaagaccg cagaaaggaa
    14161 acggtaaacc gttcatgaga aaaccaaaga taacggtgat tggtggaggg actggaattc
    14221 ccgtcattct aaaaagtctg cgggaaaaag atgtggaaat cgcagctatc gtgacggtgg
    14281 cagatgatgg tggttcttca ggtgaactcc gaaaaaatat gcaacagttg acaccgccag
    14341 gtgatcttcg taatgtcctt gtggccatgt cggatatgcc taagttttat gagaaggtct
    14401 ttcagtatcg gttctctgag gatgccggag cctttgctgg ccatccattg ggaaatctca
    14461 tcattgctgg cttgtcagaa atgcagggtt caacctataa tgccatgcag ttattgagca
    14521 aatttttcca tacaacaggg aaaatttatc cttccagtga ccatcctttg acccttcatg
    14581 cagtctttca ggatgggaca gaagtggctg gagagagtca tattgtagac catcgaggca
    14641 taattgacaa tgtctatgtg accaatgccc taaacgatga tacgcctctg gccagccgtc
    14701 gagtagtgca ggccatcctt gaaagtgaca tgattgtcct agggccaggt tccctcttta
    14761 cctctatttt gcccaatatc gtgattaagg aaattgggcg ggctcttttg gaaactaagg
    14821 cagaaattgc ctatgtctgt aatatcatga cccaacgtgg ggagacggaa cactttacag
    14881 atagcgacca cgtggaagtc ttgcatcgtc accttggtcg cccttttatc gacactgtct
    14941 tggtgaatat tgaaaaagtg cctcaggaat acatgaattc caaccgtttt gatgaatact
    15001 tagtgcaagt ggaacacgat tttgtaggtc tttgtaagca agtttcgcgc gtgatttcat
    15061 ctaacttcct tcgtctggaa aatggcggtg ccttccacga tggagatttg attgtggacg
    15121 agttgatgcg cattatacag gtgaaaaaat gagtttcaca gtagcagtaa aagaagaaat
    15181 cctgggccaa caccatctga gctggcatga attatctgcc attatcaaaa tgtctggtag
    15241 catcggtctc tcgacttcgg gcttgacttt gtcagttgtg acagaaaatg ccaaactggc
    15301 ccgtcacctc tatgagtcct ttcttcattt ctatgaaatc aaatctgaaa ttcgtcacca
    15361 ccaacgtagc aatcttcgca agaatcgcgt ctataccgtt tttacagatg aaaaggtgca
    15421 ggatttgtta agtgatttgc acttggcaga ctctttcttt ggcctggaaa caggtattga
    15481 tgaggcgatt ttatcggatg aggaagcagg tcgtgcctat ctctgtggcg ctttcttggc
    15541 aaatgggagc attcgtgacc ctgagtcagg caagtaccag ttggaaatca gttctgttta
    15601 tctggaccac gcgcaaggaa ttgcctccct tctccaacag tttttactgg atgccaaggt
    15661 gcttgagcgc aagaaggggg ctgtgaccta tctccagcga gcagaagaca ttatggactt
    15721 cttgatagta atcggagcca tgcaggcacg tgatgatttt gagcgggtta agattttgcg
    15781 agaaacccgt aacgacctca atcgggctaa taatgccgag acagctaata tcgctcggac
    15841 agtttctgcc agcatgaaga ccattaacaa tatcagtaaa atcaaagata tcatgggctt
    15901 agaaaatttg ccagtagatt tgcaggaagt ggcgcaactg aggattcagc acccagacta
    15961 ctctatccag cagttagcag atagtctcag cacccctctg accaaaagtg gtgtcaatca
    16021 cagactcaga aaaatcaata agatagccga tgaattataa aaccacgaat cctatgtgac
    16081 tcgtggttct tttttataaa ctggtagagt gttttggttg tactttttgt tcagggtcaa
    16141 tgtagttgat agcgttgttg acagcagttg gagcttctcc gaggcctgtc gcaatcagat
    16201 caatttttcc gtcatagtag cagcagtcac cgatagcata gatacctgct tggctggatt
    16261 cttgtttgct gttgacgata atcttgtgac ggttgaggtc gagcccccag tttttaaggt
    16321 taccgacaga agatttgaaa ccatagttga caaagaggtg gtctaggtca atggtttcag
    16381 tttcatcaga tttgactttt gtgatttcaa gtttatcaag tgtttttcca tttccaagga
    16441 gttggccagg ggcgaatggt gtcttgatgg ttacagatga ttcttgcaag gcttgaacac
    16501 tgtgttccaa ggcacggaaa ttatctctgc ggtgaacaag ggtagttggt gcgatttttt
    16561 caaaagccaa agcccaatcc acagccgagt ctcccccacc aagaatcgtc actttcttac
    16621 cagcgtattg ctgaatgtta gaaacgtggt agtggatatt ttcatagccc tcaacccctt
    16681 caagttccag cggacgtggt ttgaaggcac cgccacccat agcgatgata actgttttag
    16741 tcaggtgact tcctttagaa gttgtgatgg caaattcttc ttgtttgtca atctcaagaa
    16801 ccgtttcatt gagatgaata ggggtatcaa atccatttag ctgttcaatc aagcggttag
    16861 tcaactcttc tccagtcagg tttgggaagc ctggtacgtc taggatttcc ttttcagggt
    16921 agagaatagc aggttgtcca cctagctggg gaagagagtc gatgatttga accttggctt
    16981 ggcgtaggtg ggcataaaag gctgcaaaaa gcccgacagg accaccaccc acaatggtaa
    17041 tatcatagag ttgagacata gtttctcctt tgttttttct agtcagttta ttttatcata
    17101 tttttgttca atgtaaaatg gactaggata gggaaaaaaa tgcccgaaaa tggtataata
    17161 ggacgaaagt gttttgatag ggaggatatc agggatgaat tttcaacaat tatccaatct
    17221 gcaatattgg accagtttgt ttgcaagtcc atggacgata gctatcaatc tgattgatat
    17281 tttgattgtt gcttatattt tataccattt tacaaaagct attgcaggaa ccaagattat
    17341 gattttggta cgtggagttt tggtatttat tttagctcaa atccttgcaa atatgattgg
    17401 tttgactacg atttcttggt taatcaatca aattattact tatggggtta ttgcggcggt
    17461 tgttatcttc tctccagaga ttcggactgg tttggaacgt ttgggaagag cgacagattt
    17521 cttttccaat gcccctatta gtgctgagga acagatgatt cgtgcctttg ttaagtctgt
    17581 tgaatacatg agtcctcgta aaatcggggc cttggttgct attcagcgtg tacgtacctt
    17641 gcaggagtat atttcgacag gaattccctt ggatgctaag atttctgcag aacttctcat
    17701 taacattttt attcccaaca ctcccctaca tgacggtgcg gtgattatca aagaagaacg
    17761 tatcgctgtg acgtctgcct atctgccctt gacaaaaaac acagggattt ccaaggaatt
    17821 tgggaccaga caccgggcgg ctatcggttt atcagaagtc tcagatgcct tgacttttgt
    17881 cgtatcagag gaaacgggag gaatttcgat aacctataat ggaaggttta agcacaacct
    17941 aacacttgat gaatttgaaa cagaattacg tgaaatctta cttccaaaag aggaagtggg
    18001 tcttagtttt aaagaacgat tgctaggagg atggaaacat gaaaaaaaat agtttatata
    18061 tcatatcctc actctttttt gcttgtgtct tatttgtcta tgctacggcg acgaattttc
    18121 aaaacagtac cagtgctagg caggtaaaaa cggaaaccta tactaataca gtaacaaatg
    18181 tccctattga catacgctat aatagtgata agtattttat tagcggtttt gcttcagaag
    18241 tatcagtggt cttgactggt gcaaatcgcc tatcgctagc tagtgaaatg caagaaagta
    18301 cacgtaaatt caaggttact gctgacctaa cagatgccgg tgttggaacg attgaagttc
    18361 ctttgagcat tgaagattta cccaatgggc tgaccgctgt ggcgactccg caaaaaatta
    18421 cagtcaagat tggtaagaag gctcagaagg ataaggtaaa gattgtacca gagattgacc
    18481 ctagtcaaat tgatagtcgg gtgcaaattg aaaatgtcat ggtgtcagat aaagaagtgt
    18541 cgattacgag tgaccaagag acattggata gaattgataa gattatcgct gtcttgccaa
    18601 ctagcgaacg tataacaggt aattacagtg gttcagtacc tttgcaggca atcgaccgca
    18661 atggtgttgt cttaccggca gttatcactc cgtttgatac aataatgaag gtgactacaa
    18721 aaccagtagc accaagttca agcacatcaa attcaagtac aagcagttca tcggagacat
    18781 cttcgtcaac gaaagcaact agttcaaaaa cgaattaaaa atagaaaagg attttataac
    18841 aatgggtaaa tattttggga ctgatggagt ccgtggagaa gctaacctag aactaacacc
    18901 agaattagcc tttaaactag gacgttttgg aggctatgtt cttagtcaac atgaaacgga
    18961 agcgccgaaa gtctttgtag gacgtgacac acgtatttca ggggaaatgc tggaatcggc
    19021 cttggtggca ggtctccttt cagtagggat tcacgtatac aaacttggtg tccttgcaac
    19081 accagcagta gcttacttgg ttgaaactga aggagcaagt gccggtgtca tgatttctgc
    19141 tagccacaac ccagcccttg ataacggaat caagttcttt ggcggtgatg gcttcaaact
    19201 agatgatgaa aaagaagcag aaattgaagc cttgctagat gctgaggaag acactcttcc
    19261 tcgtccaagt gcagaaggct taggaatttt ggtagattat ccagaaggct tgcgtaagta
    19321 tgaaggatac cttgtttcaa ctggaactcc tcttgatgga atgaaggttg ccttggatac
    19381 agctaatgga gcagcttcta ccagtgcccg tcaaatcttt gcagaccttg gtgcccaatt
    19441 gacggttatc ggggaaacac cagacggtct taacatcaac cttaatgttg gttcaacaca
    19501 tccagaagcc cttcaagaag tggtcaaaga aagtgggtca gctattggtt tggcctttga
    19561 tggagacagt gaccgcttga ttgctgttga tgagaatggt gacatcgtcg atggtgacaa
    19621 gattatgtat atcatcggaa aatacctttc tgaaaaagga caattggctc aaaatacaat
    19681 tgtgacaact gttatgtcta accttggttt ccacaaggcc ttgaatcgcg aaggtattaa
    19741 caaggcagtt actgcagttg gtgaccgcta cgttgttgaa gaaatgagaa aatcaggcta
    19801 caaccttggt ggtgaacagt ctggtcacgt tatcttgatg gattacaata ccacaggtga
    19861 tggtcaatta tcagcagttc aattgactaa aatcatgaag gaaactggta agagcttatc
    19921 agagttggcg gcagaagtaa cgatttatcc acaaaaatta gttaatatcc gagtggaaaa
    19981 cgtcatgaag gaaaaggcca tggaagtgcc agctatcaag accatcatcg agaagatgga
    20041 agaagaaatg gcggggaacg gccgtatcct tgttcgtcca agtggaacag agcccctctt
    20101 gcgtgttatg gcagaagcgc ctacaataga agaagtggac tactatgttg ataccatcac
    20161 agatgtagtt cgtgctgaaa ttgggattga ctaaatccta gaaaactaga tgaaaataaa
    20221 aaattatggt acaatagctt ataatattgt agccgatgga gaaagacatg aatcttaaac
    20281 gtgaacaaga atttgttagt cagtatcatt ttgatgcgcg taattttgaa tgggaaaatg
    20341 aaaatggagc tcctgaaacc aaggtggatg tgaatttcca attgctccaa catgaccaag
    20401 aaaatcaagt gacttccttg attgtcatct tgagctttat gattgtattt gataagtttg
    20461 tcattagtgg aacaatctca caagtaaacc atatcgatgg ccgtattgtc aatgaaccaa
    20521 gtgaattgaa ccaagaagag gtggaaacct tggctcgtcc atgtttgaac atgctcaatc
    20581 gtttgactta cgaagtgact gaaattgcat tagacttacc aggaattaat ttggagttct
    20641 aatatgaaat tagctgtttt cacagattcc tctgcttatc tcagtgaaga gaccttgcaa
    20701 agagaagact tgtttgtctt ggatattcct gtcaatattg atggtgagga atatgtcgaa
    20761 ggcatcaatc tgtctgctga ggaattttac caaaaaatgg ctcaggcttc tgaattgcct
    20821 aagaccagtc aaccaagtat tgccaagtta gatgagatct taacttcgct caaagaacaa
    20881 ggctatacac atgccttggg gcttttccta tcttctggaa tttcaggttt ttaccaaaat
    20941 atccagtata tggtcgatga ctatgagggc ttaaccattg ctttcccaga cactttgatt
    21001 acaagtgctc ccctaggcat catggttgaa agcgtcttta attggcgtga tcagggcgat
    21061 gattttgcca gcattcagga taagctagcc attcaaatca gccgtacgtc agcctttatc
    21121 atggtagatg acttggatca tttggtaaaa ggtggacgcc tttcaaatgg ggctgccatt
    21181 ttgggcaatt tgcttagcat taagccaatc ctttatttta acgatcaagg tgtgattgaa
    21241 gtttacgaaa aagttcgtac tgaaaagaag gccaccaaac gcttaattga aattatcaag
    21301 gaaacaacgg cttcaggtca ataccgggtc attgtcattc atgggaatgc tcctgaaaag
    21361 gctgaagaat tgcgtcagca tttgcttgat tttggcttgg gttcggatgt ttcacttgct
    21421 acatttggta gtgtcattgg aacgcaccta ggagcaggaa gtattgctct gggttatatt
    21481 ccagtgattt agtaggcact tctaggctag ttaatcttag caattgaaca cggactacct
    21541 gcctcttgaa aaaagatgtc tgtcttgcct ttcgtggaaa gtcagtgcca ttccctattt
    21601 ttcatgggtg gctaagatcc tttgtatttt gataattgaa cacgcctgga accctgtgtg
    21661 aaaaagatag ttcttccaag gagtagacac ttcttgatca gaattcctat tttcactttg
    21721 tgttcttaca ggctttgtat cttagacagg agtagagatg agtattcgag taattattgc
    21781 cggttttaag ggaaagatgg gtcaagctgc ttgccagatg gtcttgcctg attcagattt
    21841 ggacttggta gcagttttgg atccttttga gtctgagtca gaatggcagg gtattcctgt
    21901 tttcaaggat aaggctgatt tagctggttt tgaagcggat gtctgggtag actttactac
    21961 tccagctgtt gcctacgaaa atacacgttt tgctcttgaa aatggctttg ctccagtagt
    22021 tggaacgact ggtttcacga gtgaagaaat tgcagagcta aaagaatttt ctcgtgccca
    22081 agacttgggt ggcctgattg cccctaactt tgccttgggt gctgtcttac tcatgcaatt
    22141 tgcgacgcag gctgccaaat atttcccaaa tgtggagatt attgagctcc atcatgacaa
    22201 gaaaaaggat gctccgagtg gaacagccat taaaacagct gagttgatgg cagaggttcg
    22261 agagtcaatt cagcaaggtg cagcagatga ggaagagctg attgctggtg ctcgtggtgc
    22321 tgactttgat ggtatgcgca tccactcagt tcgtttgcca ggcttggtag ctcatcagga
    22381 agtcatcttt ggcaatcagg gagaagggtt gaccctccgt catgactcct atgatcgcat
    22441 ctccttcatg acaggagtca atttgggaat taaagaagtt gtcaagcgtc atgagcttgt
    22501 ctatggatta gaacacttat tatgagatta acgcaaatgc cttctgaatt tcagaaggct
    22561 ttaccagtat tagaaaaaat taaagaagca ggctttgagg cttattttgt tgggggctct
    22621 gttcgagatg cccttctcca tagccctatc cacgatgtgg atattgcgac gtcttcttat
    22681 ccagaagaga ccaagcagat ttttccgcga acagccgata tcggaatcga gcatggaacc
    22741 gtcttggtct tagatgggga tgaggagtat gaggtaacaa cctttcggac agaggatgtc
    22801 tatgtggact atcgcagacc cagtgcggtt tcctttgtgc gctcgctaga agaagacctc
    22861 aaacgccgtg atttcacagt caacgccttt gccttggatg agacaggaga aatcgttgac
    22921 ttgttccatg gtttagaaga tttggaaaag caagtcttgc gagcagttgg agtggctagt
    22981 gagcgtttca acgaagatgc tttacggatt atgcgtggtt tccgttttca ggctagtctt
    23041 ggttttgcac ttgagccaga aacatttaaa gctatgaaga ccttgacgcc gcttttggag
    23101 aaaatttctg tagagcgtac cttcgtcgaa tttgataaac tcttgctggc tccattttgg
    23161 agaaggggct tggcttccat gattgagagt caagcttatg actatctccc tgatatggca
    23221 tctagccagg acaagctcaa cagactgttt gatttggaga ctgattttac ttttgaatcc
    23281 tctgaacaag cctgggcggc tttactatgg gctttggaga ttgaaaatgc gcagtcattt
    23341 ttgaaatctt ggaagacctc acgccagttt gccaagcaag ttcaggattt gctgattatt
    23401 ttggctctgc gtgaaaatgg agaattgagc aagcgagatt gttatcgctt tgacatagat
    23461 ttgcttttac aggctgaaaa tcttcgtcaa gctcaaggaa aagaagtcaa cccacaagcc
    23521 atcacagaaa aataccaaag cttgactatc catgacaaga aagagattca gattaatggc
    23581 gatattttga tcaaggaata tggctatcag ccaggcccag acttgggaga gattttaaca
    23641 gagattgagt ttgccattgt cgatggagaa ttggagaata atcgtgaagc catccatgct
    23701 tacctgaggg agaaaaaatg agtgatttta tcgttgaaaa actaagtaaa tccgttggtg
    23761 acaagaccgt ttttagggat atttccttta ttatccatga cttagacaga attggtttaa
    23821 tcggtgtcaa tgggactggc aagaccaccc ttttggacgt cctttctggt gtttctggat
    23881 ttgatgggga tgtcagtcct ttttcagcta aaaatgatta ccagattggt tatttgactc
    23941 aggatcctga ttttgatgat agaaagacag ttttggatac ggttctatct agtgaactca
    24001 aggaaatcca gctcattcgt gagtatgaat tgattatgct cgactatagt gaggacaagc
    24061 aggcgcgttt ggaacgtgtc atggcagaga tggactctct ccaagcttgg gaaatcgaga
    24121 gtcaggtcaa gaccgttctt agcaaattgg gcattcaaga cttatctact cctgttgggg
    24181 aattgtcagg tggtctgaga agacgggtac agttggcaca agtcttactt ggcaaccacg
    24241 accttttgct tttggatgag ccgaccaacc atctggatat tgcgattatt gagtggctga
    24301 ccctcttttt gaaaaattct aagaagaccg tcctttttat cactcacgat cgttatttct
    24361 tagacgcttt gtcaacacgg attttcgagt tggagcgagc aggcttgaca gaatatcagg
    24421 gcaattacca ggactatgtt cgccttaagg cagaacagga cgagcgcgat gcagctctac
    24481 ttcataaaaa agaacaactc tacaaacaag aactggcctg gatgagcaga caaccgcagg
    24541 cgcgtgcgac caagcaacaa gctcgtatca atcgtttcca tgatctgaaa aaggaagttt
    24601 caggcagtag tgctgagaca gacttgacta tgaactttga aaccagtcgg attgggaaga
    24661 aagtcatcga gtttcaggct gtttcctttg cctatgaaaa taagcccatt ttgcaagatt
    24721 ttaatctctt agttcaggtt aaagaccgta ttggaattgt tggggacaat ggtgttggaa
    24781 aatcaaccct acttaacctg attgcaggaa gtcttgagcc gacagcagga caagttgtga
    24841 ttggggaaac tgttcgcatc gcctatttct ctcaacaaat tgagggtttg gatgaaagca
    24901 agcgtgtgat caattacctg caggaagtgg cagaggaggt caagaccagt ggtggttcta
    24961 cgacttccat cgctgagttg ctggagcaat tcctcttccc acgttcgacg catgggacct
    25021 tgattgagaa gttgtcaggg ggtgagaaaa aacgtcttta tctcctcaaa ctgcttttgg
    25081 aaaaaccaaa tgttcttctt ttagacgagc caaccaatga cctagatatt gcaactttga
    25141 cagtcttaga gaatttcttg caaggttttg caggtcccgt tttaacagtc agtcacgacc
    25201 gctatttctt ggataaggta gcgaccaaga ttctcgcttt tgaggatggc aagattcgtc
    25261 ctttctttgg tcattacacc gactatcttg atgaaaaagc ttttgaaaca gatatggcca
    25321 atcaagtgca aaaggccgaa aaggaaaaag tggtcaaggt tcgagaagac aagaaacgca
    25381 tgacctacca agaaaagcag gagtgggcaa gtattgaagg tgatattgaa accttggaaa
    25441 aacgtatcgc tgctattgaa gaggaaatgc aggctaacgg ctctgacttt ggtaagctgg
    25501 ctactctcca aaaagaattg gatgagaaaa atgaagcact ccttgaaaaa tacgaacgct
    25561 atgagtatct cagtgaattt gatagttaag actattcgaa aatcaaagtg caaactatgt
    25621 cggcttcaca ttgccgactc aagtacagcc tgtgaccggc ttcctaattt gcactttgat
    25681 ttttattgag tataaaagaa tagaaaaatc ccgtctcatg aaaggatttc tctattcttt
    25741 tttggtttct tgatgaaaga tattttgcca ggtttcgtgg cgacgccaga tactggtatg
    25801 gatgatacca tctaactcat agcagatgag tttggttttt tgactgatgg aagtaatttg
    25861 aatgtccttg atagcagaat ttaactcttt tttggtctta taagcctctt tatccatctg
    25921 ctctccatct tggcgaatat agacaaaatc atcagtcaag agttcttcta gttggtttcc
    25981 ttggtcaatc aattgttcac gcataagcaa ttttttatag acattgtcta aaatttcatc
    26041 ctcagggata ggtgctggtt cgatatggac atcggtatca aagacgccaa aacgctcctc
    26101 cagcatagac tcgacctgat ccgcgatttc atggctttca aaaacagaca agtcaggatt
    26161 catctctagt gtaatatcca ggtagatgtt gctaccgtag gtgcgacctc tttgcgattt
    26221 gaccttgctg attttgggaa tttccatgat agccttttgg tagtcctcga gcaggcggtc
    26281 gtcaaagcca tctgaaagac taaaggaaga ctcgatgaag atatcatagg cagtcttcaa
    26341 gataaagaaa gtgatgatga tagcaaccag tttatccaca atcggataat tgaaactact
    26401 agctaggatg gcaatggcgg ttccaagtga ggtaacagcg tcagaaagat tgtccttagc
    26461 agctgccttc agcgccttgg agttggattt cttactgagg cgagtattgt agagatagac
    26521 cacaaacata atcgctgcag aaatgattcc tagagttgca ccaagaggat caatgaccgt
    26581 ttcttcccga ctgagaatct tttgaatggt atctcttaga acatcgaaac cgacatagaa
    26641 catgatgata gaagtgatca agcttgccaa atcttcaatc ttccaatgac caaaacggtg
    26701 gtcacggtct gcaggctggc gcgccatccg aatcccgatt aagagggcca catttccaat
    26761 gatgtccgat acgttattaa aaccatcggc caccaaactg gatgaatgaa ggagatgacc
    26821 agctgctaat ttggctgcag acaagatcaa ataggtcgaa atactgataa tggctccacg
    26881 ctcagctaac ttgagatttg agatagattg cttcattcaa cttcctttct agtcacatag
    26941 cattctacca ttatactggt tttcaaggaa aaattcaaat aaggcagttt ttactctttg
    27001 aaaactcccc aaaatttggt ataatggtac tactcaaggg agtagctggc agaaacctgt
    27061 gatagtgtcg tcattccgaa ttttatgctg aaaagtatgc tttccggccc tatcttaaac
    27121 agcgagactt gttatgatta acaggtctct ttttgtttgt cgtgaaaaga tatgacagaa
    27181 agagagtctg ttttgcacac aatgtcaagg aggagacaca tgtcaaaaga acaaaaacgc
    27241 caagcgtttt atactcagag ccctgaagag gtcttgcagg ctgtggatgc gaccgagcaa
    27301 ggtttgtcat caagtgaggc ggaaaagcgc cttgccgaat ttgggcacaa tgaactcgaa
    27361 gaaggcgaga aacgatcaat cttggtcaaa ttcatcgagc aatttaagga tttgatgatt
    27421 atcatcctag ttgcggcagc aatcttgtca gtcgtgactt ctggtgggga agatatcgca
    27481 gatgccatta tcatcctagc tgtggttatc atcaacgctg cctttggtgt ttaccaagaa
    27541 ggaaaagctg aagaagctat tgaagccctc aaatccatgt ctagtccagt tgcccgcgtt
    27601 cttcgtgatg gacacatggc agagattgac tctaaagaat tggtaccagg cgatatcgtt
    27661 gcccttgaag caggtgacgt ggtaccagcg gacctacgtt tgatagaagc caactctctt
    27721 aaaattgaag aagcagcctt gacaggtgaa tctgtaccag tcgaaaaaga cttgtcagtc
    27781 gagcttgcga cagatgctgg tattggtgac cgtgtcaaca tggccttcca aaactcaaac
    27841 gtaacctatg gtcgtgggat gggtgttgtt gtcaatacag gtatgtacac tgaagttggt
    27901 catattgctg gtatgcttca agatgcggat gagactgata caccactcaa acaaaatttg
    27961 aacaaccttt ctaaggtctt gacctatgct atcttggtca ttgcccttgt tacttttgta
    28021 gtgggtgtct tcattcaagg gaaaaatcca cttggtgagt tgttgacttc tgttgccctt
    28081 gccgttgcag ccattccaga aggacttcct gctatcgtta ccatcgttct ttctcttggt
    28141 actcaagttt tggccaaacg acattccatc gttcgtaagt tgccagcagt tgaaacactt
    28201 ggttcaactg aaatcatcgc ttctgataag actggtacgc tgactatgaa caagatgaca
    28261 gtcgaaaaag tcttctacga tgcggttcta catgactcag ctgatgatat tgaactaggt
    28321 cttgaaatgc cactacttcg ttcagttatc ttggccaatg atacgaaaat cgatgtggaa
    28381 ggtaacttga ttggtgaccc aaccgaaaca gcctttatcc aatatgcctt ggacaagggc
    28441 tatgatgtca aaggtttctt agagaaatat cctcgtgtag ctgaattgcc atttgactct
    28501 gaccgtaagc tcatgtcaac agttcaccca ttgccagatg gtcgtttcct tgtagcagtc
    28561 aagggtgcgc cagaccaact cttaaaacgt tgtcttcttc gtgataaggc tggggatatt
    28621 gctccgattg atgagaaggt tacaaatctc attcatacaa acaattctga aatggctcat
    28681 caagccttgc gtgtccttgc aggtgcttat aagattatcg atagtattcc agaaaatctc
    28741 acttctgaag agcttgaaaa tgatttaatt tttactggtt tgattgggat gattgatcct
    28801 gaacgtcctg aagctgctga ggctgttcgt gtggctaagg aagctggaat tcgtccaatt
    28861 atgattacag gtgaccacca agacaccgca gaagctattg ccaaacgttt gggaatcatt
    28921 gacgcaaacg atacagaaga ccacgtttta actggtgctg agctcaacga attatctgat
    28981 gaagactttg aaaaagttgt tggtcaatac tctgtttatg cccgtgtgtc tccagagcac
    29041 aaggttcgta tcgtcaaggc ttggcaaaaa caaggtaagg tcgttgccat gacaggtgac
    29101 ggtgtcaatg atgccccagc tctgaaaaca gcagacatcg gtatcggtat gggaatcact
    29161 ggtacagagg tttctaaggg ggcttctgat atgattcttg cagatgataa ctttgcgact
    29221 attatcgtcg cagtggaaga aggacgtaag gtcttctcaa atattcaaaa gactattcag
    29281 tacctacttt ctgccaatac tgctgaagta ttaaccatct tcctatcaac cttgtttggt
    29341 tgggacgtct tgcagccagt tcatcttttg tggattaact tggtaacgga taccttccca
    29401 gcaattgcac taggtgttga acctgcggaa cctggtgtca tgaatcataa accacgtgga
    29461 cgcaaggcaa gcttcttctc aggtggtgtt ttgagttcta tcatttatca aggtgtactc
    29521 caagcagctc ttgttatgag tgtttatggc cttgcgattg cttacccagt tcatgtgggt
    29581 gacaatcatg ctattcatgc agatgcccta acgatggcct ttgcaaccct tggtttgatt
    29641 cagctcttcc atgcctacaa tgtcaagtct gtctaccaat ccatcttgac agttggccca
    29701 ttcaagtcta agacctttaa ctggtccatc ttggtatcct ttatccttct catggcaaca
    29761 atcgttgtag aaccgcttga aggaatcttc cacgtaacca aactagactt gtcacaatgg
    29821 ggaattgtta tggctggaag cttctcaatg attatcatcg tcgaaatcgt taagtttatt
    29881 caacgcaaac tcggttttga caagaatgcg atttaaggga ggtttcggat gtcagcatat
    29941 caattaccga ccgtatggca ggatgaagct agtaatcaag gagcttttac ggggttaaac
    30001 agaccaacag caggtgcccg tttcgaacaa aacttgccaa aaggagaaca agcttttcag
    30061 ctttattcac tgggaacacc aaatggtgtg aaggttacta tcttattgga agaattacta
    30121 gaagctggtt ttaaggaagc ggcttacgac ttgtataaga ttgctatcat ggatggggat
    30181 caattcggct cagactttgt gaagctcaat ccaaattcca agattccagc cttattggac
    30241 cagtcaggta ctgaaaacgt aagagtcttt gagtctgctc atattcttct ttaccttgct
    30301 gagaaatttg gagccttttt accaagtaat cctgtggaaa aggtagaagt tttgaattgg
    30361 ctattctggc aagcaggtgc agcacctttt ctaggtgggg gatttggaca tttcttcaat
    30421 tatgctcctg aaaaattgga atatcctatt aaccgtttta cgatggaagt gaaacgccag
    30481 ttggatttat tggataagga attggctcag aaaccttata ttgcaggcaa tgactatacg
    30541 attgcagata ttgctatctg gtcttggtat ggacagttag ttcaaggaaa tctttaccaa
    30601 ggttctgcaa aatttttgga tgcctcaagt tatcaaaatc tagtaaaatg ggcagaaaaa
    30661 attgccaatc gtccagctgt taagcgtgga ttggaagtaa cttatacaga aattaaatag
    30721 caaaaaagtc atcttcggat ggctttttgc tacagttgag ggaaagggtt tgcagttatc
    30781 ggcttttgtg ctataattgc tataatatag taaaaagcaa gaggagtgtg aggaagtgga
    30841 aaagaaaatt gtgaaggata tcttattttt atctcaagtg tctcagccgg caagtcagga
    30901 ggacctttat cttgccagag atttgcagga tacactctta gcaaatcgtg atacctgtgt
    30961 tggtctagct gccaatatga ttggggtgca gaagcgcgtg attatcttta atcttggctt
    31021 ggttcccgtg gtcatgttta acccagtgct tctgtccttt gaaggatctt atgaggcaga
    31081 agaaggctgt ttgtccttgg taggtgtgag atcaactaag cgttatgaaa ccataaggct
    31141 tgcctatcgt gacagcaagt ggcaggaaca gaccattacc ttgacaggct tcccagctca
    31201 gatttgccag catgagctgg atcacttgga aggacgaatc atttaggagg aaagcaaatg
    31261 aaacgaatag tctttgaact tatttttatc gcaacgacct ggtatatctt tttaccgccc
    31321 cttaacctga ccagctggga atttctcttc ttcctctgtg ggcatttgtt agttgtggca
    31381 atcttatttg gctttggcaa ggggataaac cttgtcaaaa cggttcatgt gcgccacggt
    31441 aaggcggaag ctgccttaaa tcttgagggt ttcaaaatca atcggttagg gaaaattctg
    31501 ttagcttcga ttggaggaat tcttctcttg gcagctttgg tttccttggt aacttccagc
    31561 atgtttcagg ctaaaaatta tgccaatgta gtcacggtta cggaaaaaga ctttactgaa
    31621 tttcctagga gtgacaccag taaggttcct atcctagata gaagtactgc tgaaaaaatt
    31681 ggagaccgct acttgggttc cctaaccgat aaggtgtcgc aatacgtagc ggcagatacc
    31741 tatacccaat tgacaattga tgggaaacct tatcgggtca caccactaga atatgcagac
    31801 cctatcaaat ggtttaacaa tcaagccaag ggaatcggtg agtatatcaa ggtggacatg
    31861 gtaactggaa atgcggattt ggtggacttg aagacaccaa tcaagtattc agactcggag
    31921 tattttaacc gtgatgtcaa acgtcacctg cgcttgaagt acccgactaa aatctttaaa
    31981 actccatctt ttgaggtgga cgatgagggc aatcctttct atgtagcaac ggtttaccaa
    32041 aagcaatttg gacttgcggt tcctcgtcct gcttcagtca ttatcttgga tgctacaaat
    32101 ggagaaacca aggaatacag cttatcagat gttccagaat gggtggacag ggtctatcca
    32161 gcagaggaaa ccattgagca aatcaactac aacggcaagt acaaggacgg tttcttgaat
    32221 gccatgattt ccaagaaaaa tgtgacccag actaccaagg gctataatta cttgtctatc
    32281 ggtaatgaca tctatctcta cacaggtgtg acgtcggcta atgcggatga gagtaatctt
    32341 ggtttcatcc ttgaaaatat gcgaacagga gaaatcacta agtatagctt ggcttctgcg
    32401 acagaagaat cagcccgtga atcagcagaa ggtgctgttc aggagaaatc ctacaaagca
    32461 accttcccaa tcctcatcaa cctcaatgac aagcctctct acatcatggg cttaaaggac
    32521 aatgctggct tggtcaaaga gtacgccctg gtagacgcag tcgagtacca aaatgttatc
    32581 gttgctacta cagtggaaga gatgctcagc aagtatgcca ataaaaacga ccttgaaatt
    32641 gacaatgcaa cgacagaaag catcaaggga gtagtagcag acctcaaatc agctgttatc
    32701 aagggagaca ctgtctactt ctttaaagtt gatggcaaga tatacaaggt caaggcttca
    32761 gtatccgatg accttcctta ccttgaaaat ggtaaaacct tcgaaggtca agtaggaaaa
    32821 gacaattatc tcaagacctt taaagtacaa taatactctt cgaaaatctc tagctctatc
    32881 tgcaacctca aaacagtgtt ttgagcag
//
LOCUS       DBERCA010000018        27633 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.018, whole genome shotgun sequence.
ACCESSION   DBERCA010000018 DBERCA010000000
VERSION     DBERCA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.018"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(191..478)
                     /locus_tag="ACL719_000965"
                     /pseudo
     CDS             complement(191..478)
                     /locus_tag="ACL719_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            477..740
                     /locus_tag="ACL719_000966"
                     /pseudo
     CDS             477..740
                     /locus_tag="ACL719_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            787..1485
                     /locus_tag="ACL719_000967"
     CDS             787..1485
                     /locus_tag="ACL719_000967"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS0698959.1"
                     /translation="MSQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            1504..3021
                     /locus_tag="ACL719_000968"
     CDS             1504..3021
                     /locus_tag="ACL719_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS0698960.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTSGRGEEVDSKEISESADSTSNIADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            3146..4474
                     /locus_tag="ACL719_000969"
     CDS             3146..4474
                     /locus_tag="ACL719_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0698961.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            4726..5592
                     /locus_tag="ACL719_000970"
     CDS             4726..5592
                     /locus_tag="ACL719_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698962.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            5608..6447
                     /locus_tag="ACL719_000971"
     CDS             5608..6447
                     /locus_tag="ACL719_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0698963.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            6720..7172
                     /locus_tag="ACL719_000972"
     CDS             6720..7172
                     /locus_tag="ACL719_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS0698964.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            7269..7613
                     /locus_tag="ACL719_000973"
     CDS             7269..7613
                     /locus_tag="ACL719_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698965.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIIRGIVNGLIGRYIKLGTY
                     AAKYGISMARSILSRAAATAAARVRLLTKIFGWILRIAVLAADVYGNFANNIAAAWDA
                     HDKIPNNGRINF"
     gene            7623..7817
                     /locus_tag="ACL719_000974"
     CDS             7623..7817
                     /locus_tag="ACL719_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0698966.1"
                     /translation="MKALCIFLLYMLAWTVLAMIIRGGLDGFDEHTWRNILASALSSL
                     YIHYISIDRKRKKSKRKKKK"
     gene            7900..8544
                     /locus_tag="ACL719_000975"
     CDS             7900..8544
                     /locus_tag="ACL719_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS0698967.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            8713..9630
                     /locus_tag="ACL719_000976"
     CDS             8713..9630
                     /locus_tag="ACL719_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGS0698968.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKDKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            9648..10532
                     /locus_tag="ACL719_000977"
     CDS             9648..10532
                     /locus_tag="ACL719_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS0698969.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(10548..11399)
                     /locus_tag="ACL719_000978"
     CDS             complement(10548..11399)
                     /locus_tag="ACL719_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS0698970.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDGNCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            11599..13656
                     /gene="pbp2b"
                     /locus_tag="ACL719_000979"
     CDS             11599..13656
                     /gene="pbp2b"
                     /locus_tag="ACL719_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGS0698971.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            13667..14263
                     /gene="recR"
                     /locus_tag="ACL719_000980"
     CDS             13667..14263
                     /gene="recR"
                     /locus_tag="ACL719_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGS0698972.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            14439..15482
                     /locus_tag="ACL719_000981"
     CDS             14439..15482
                     /locus_tag="ACL719_000981"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGS0698973.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            15566..16939
                     /gene="murF"
                     /locus_tag="ACL719_000982"
     CDS             15566..16939
                     /gene="murF"
                     /locus_tag="ACL719_000982"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGS0698974.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAYLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            16926..17537
                     /locus_tag="ACL719_000983"
     CDS             16926..17537
                     /locus_tag="ACL719_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGS0698975.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEGSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDINRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPVLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            17628..18332
                     /locus_tag="ACL719_000984"
     CDS             17628..18332
                     /locus_tag="ACL719_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGS0698976.1"
                     /translation="MNLWDIFFTTQATKPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIVKEA"
     gene            18551..19924
                     /gene="ftsA"
                     /locus_tag="ACL719_000985"
     CDS             18551..19924
                     /gene="ftsA"
                     /locus_tag="ACL719_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGS0698977.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            19941..21200
                     /gene="ftsZ"
                     /locus_tag="ACL719_000986"
     CDS             19941..21200
                     /gene="ftsZ"
                     /locus_tag="ACL719_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGS0698978.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            21205..21876
                     /locus_tag="ACL719_000987"
     CDS             21205..21876
                     /locus_tag="ACL719_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGS0698979.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYIGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            21886..22425
                     /locus_tag="ACL719_000988"
     CDS             21886..22425
                     /locus_tag="ACL719_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGS0698980.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            22425..22688
                     /locus_tag="ACL719_000989"
     CDS             22425..22688
                     /locus_tag="ACL719_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGS0698981.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            22685..23470
                     /locus_tag="ACL719_000990"
     CDS             22685..23470
                     /locus_tag="ACL719_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGS0698982.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            23479..24267
                     /gene="divIVA"
                     /locus_tag="ACL719_000991"
     CDS             23479..24267
                     /gene="divIVA"
                     /locus_tag="ACL719_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGS0698983.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            24615..27407
                     /gene="ileS"
                     /locus_tag="ACL719_000992"
     CDS             24615..27407
                     /gene="ileS"
                     /locus_tag="ACL719_000992"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGS0698984.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEYGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         8356 a         5055 c         6074 g         8148 t
ORIGIN      
        1 aggaaataat aaaaatctat gggtaatgtt ctgaaagtac ttttaggtta ggagagaaat
       61 ataatgattt ttgaaaattg attcaaaaaa tagcgaattg tagaaaacgc ttaacaaatg
      121 gtgtataata gacttccagc gaaacaaaat atgctacaat aaacctatga attataaagc
      181 aaccttatac tcatctgtct tcacaaagca ttctagtcca tctccgatta acgatggact
      241 ttatcatctc cttctccagt ccttgtatga catcttccaa agttcgaaag gctttattct
      301 taaatccacg tttacgaatc tctttccaca cttattcaat ggggttcatt tctggtgtgt
      361 atggaggaat aaatgcaaag ccaatattag tcggaatctt taaggcactt gatttatgcc
      421 atatagcatt gtccataacg aataaaagat aatcatctgg ataagcttgt gaaagcttgg
      481 ctgacagtgg ttatcaaggg ctcatgaaga tatatcttca agcacaaact ccacgtaaat
      541 ccagcaaact caagccgcta acagctgaag ataaagccta taaccatgcg ctatctaagg
      601 agagaagcaa ggttgagaat atttttgcca aagtaaaaac gtttaaaatg ttttcaacaa
      661 cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct gatattatca
      721 atcatgaact aggattctag ttttgcagga agtctaataa atgtataaga aaaaggagga
      781 aaaaggatgt cacagattag caaagaagcc ttgattgagc aaatcaaaga tggaatcatc
      841 gtttcttgtc aggctcttcc tcatgaaccg ctttatacag aagcgggagg ggtgattccc
      901 ttgctggtca aagcggctga gcaaggtgga gcagtcggta tccgagcaaa cagtgttcgc
      961 gatatcaagg aaattaagga agtcactaaa cttccaatca ttgggattat caaacgtgat
     1021 tatccacctc aggaaccctt catcacggct actatgaaag aagttgatga attggcagaa
     1081 ctggacatcg aggtgattgc tctggattgt accaagcgtg aacgctacga tggtttggaa
     1141 attcaagagt tcattcgtca ggttaaggag aaatatccta atcagctttt gatggctgat
     1201 actagtatct tcgaagaagg gctagcagct gtagaagcag gaattgactt tgtcggaaca
     1261 accttatcag gctacacatc ctacagtcca aaagtagacg gtccagattt tgaattgatt
     1321 aagaaactct gtgatgccgg tgtagatgtc attgcagaag gaaaaattca tacaccagaa
     1381 caagccaaac aaatccttga atatggagtg cgaggcatcg ttgttggtgg cgccattact
     1441 agaccaaaag agattacaga acgcttcgtt gctagtctta aataagatgt gagggggagt
     1501 tttatgttta aagttttaca aaaagttgga aaagctttta tgttacctat agctatactt
     1561 cctgcagcag gtctactttt ggggattggt ggtgcacttt caaacccaac cacgatagca
     1621 acttatccaa tactagacaa tagtattttt caatcaatat tccaagtaat gagctctgca
     1681 ggagaggttg tattcagtaa tttgtcacta cttctctgtg tgggattatg tattggctta
     1741 gcgaaacgag ataaaggaac cgctgcgtta gcaggagtaa ctggttactt agttatgact
     1801 gcaacgatca aagctttagt aaaacttttt atggcagaag gatctgcaat tgatactgga
     1861 gttattggag cattagttgt cggaatagtt gccgtatatt tgcacaaccg atataacaat
     1921 attcaattac cttccgcttt aggattcttt ggaggttcac gcttcgttcc tattgttaca
     1981 tcgttctctt ctatcttgat tggctttgtc ttctttgtta tttggccacc tttccaacaa
     2041 cttcttgttt ctacaggtgg atatatttct caggcgggtc caattggaac ttttctatat
     2101 ggatttttaa tgagactttc tggagcagta ggcttacatc atataattta ccctatgttt
     2161 tggtatactg aacttggtgg tgttgaaact gttgcaggac aaacagtggt tggagctcaa
     2221 aaaatatttt ttgctcaatt agccgatttg gcccattctg gattatttac agaaggaaca
     2281 aggttttttg caggtcgttt ctcaacaatg atgttcggtt taccggctgc ctgtttagcg
     2341 atgtaccata gtgttcctaa aaatcgtcgt aaaaaatacg cgggtttgtt ttttggagtt
     2401 gctttaacat cttttattac cggtattaca gaaccaattg aatttatgtt tctattcgtc
     2461 agtccggttc tatatgttgt tcacgcattc cttgatggtg ttagcttctt tattgcagac
     2521 gtcttaaata tttcaatagg aaacacattt tcaggaggtg taatcgattt cactttattt
     2581 ggaattttgc aggggaacgc taagacgaat tgggttcttc agattccatt tggacttatt
     2641 tggagtgttt tgtattatat tatttttaga tggttcatta ctcaattcaa cgttctaacg
     2701 tcaggacgag gagaagaagt agattctaaa gaaatttctg aatccgcaga ttcaacttca
     2761 aatattgcag attatttaaa acaggatagc ctacaaatta tcagagcctt gggtggatca
     2821 aataatatag aagatgtaga tgcttgtgtg acacgtttac gtgtagctgt aaaagaagtt
     2881 aatcaagttg ataaagcact tttaaaacaa attggtgcag ttgatgtctt agaagtgaag
     2941 ggtggcattc aagcaatcta tggagcaaaa gcaatcttat ataaaaatag tattaatgaa
     3001 attttaggtg tagatgatta agtacttact gacttaataa aaaacagagg agagtgatgg
     3061 atgagtagga tgaaatgaaa tcgcatacaa gaaataaaga actcattatc caagttggat
     3121 acgcttatta cataggagaa tacaaatgaa atttagaaaa ttagcttgta cagtacttgc
     3181 gggtgctgcg gttcttggtc ttgctgcttg tggcaattct ggcggaagta aagatgctgc
     3241 caaatcaggt ggtgacggtg ccaaaacaga aatcacttgg tgggcattcc cagtatttac
     3301 ccaagaaaaa actggtgacg gtgttggaac ttatgaaaaa tcaatcatcg aagcgtttga
     3361 aaaagcaaac ccagatataa aagtgaaatt ggaaaccatc gacttcaagt caggtcctga
     3421 aaaaatcaca acagccatcg aagcaggaac agctccagac gtactctttg atgcaccagg
     3481 acgtatcatc caatacggta aaaacggtaa attggctgag ttgaacgacc tcttcacaga
     3541 tgaatttgtt aaagatgtca acaatgaaaa catcgtacaa gcaagtaaag ctggagacaa
     3601 ggcttatatg tatccgatta gttctgcccc attctacatg gcaatgaaca agaaaatgtt
     3661 agaagatgct ggagtagcaa accttgtaaa agaaggttgg acaactgatg attttgaaaa
     3721 agtattgaaa gcacttaaag acaagggtta cacaccaggt tcattgttca gttctggtca
     3781 agggggagac caaggaacac gtgcctttat ctctaacctt tatagcggtt ctgtaacaga
     3841 tgaaaaagtt agcaaatata caactgatga tcctaaattc gtcaaaggtc ttgaaaaagc
     3901 aactagctgg attaaagaca atttgatcaa taatggttca caatttgacg gtggggcaga
     3961 tatccaaaac tttgccaacg gtcaaacatc ttacacaatc ctttgggcac cagctcaaaa
     4021 tggtatccaa gctaaacttt tagaagcaag taaggtagaa gtggtagaag taccattccc
     4081 atcagacgaa ggtaagccag ctcttgagta ccttgtaaac gggtttgcag tattcaacaa
     4141 taaagacgac aagaaagtcg ctgcatctaa gaaattcatc cagtttatcg cagatgacaa
     4201 agagtgggga cctaaagacg tagttcgtac aggtgctttc ccagtccgta cttcatttgg
     4261 aaaactttat gaagacaaac gcatggaaac aatcagcggc tggactcaat actactcacc
     4321 atactacaac actattgatg gatttgctga aatgagaaca ctttggttcc caatgttgca
     4381 atctgtatca aatggtgacg aaaaaccagc agatgctttg aaagccttca ctgaaaaagc
     4441 gaacgaaaca atcaaaaaag ctatgaaaca atagtcctta gttattctat aaaaagtagt
     4501 tttttaaaga acctaagagt gtataccccc ttttccctct acacagatag tgtaagaaaa
     4561 gggggctttt gtttaaaatg taagaaactg tcacgaaatt aaaatgaagt tcttacataa
     4621 gcgaaccata aaaaatttca ttttgatttt aaaacagttc aagaaagtca aaaaattatt
     4681 ctatttgaaa gagaggtgcc gactgtgaaa gtcaataaaa tccgtatgcg ggaaacagtg
     4741 atttcctacg ctttcctagc accagtatta ttcttctttg tcatctttgt gttggctccg
     4801 atggtgatgg gcttcattac aagtttcttt aactactcaa tgactaaatt tgagtttgtg
     4861 ggcttagata actatatccg tatgtttaaa gatcctgtct ttacaaaatc tctgattaac
     4921 acagttattt tggttattgg atctgtacca gttgttgttc tattctcact ctttgtagca
     4981 tctcagacct atcatcaaaa tgtcattgcc agatccttct accgtttcgt cttcttcctt
     5041 cctgttgtaa cgggtagtgt tgccgtgaca gttgtttgga aatggattta tgacccgcta
     5101 tcagggattc taaactttgt ccttaagtca agccacatca tcagccaaaa catttcttgg
     5161 ttgggagata aaaactgggc attgatggcg attatgatta ttctcttgac cacttcagtt
     5221 ggtcagccca tcatccttta tatcgctgcc atggggaata ttgacaattc actggttgaa
     5281 gcggcgcgtg ttgatggcgc aactgagttt caagtttttt ggaagattaa atggccaagc
     5341 cttcttccaa caactcttta tattgcaatc atcacaacaa ttaactcatt ccagtgtttc
     5401 gccttgattc agcttttgac atctggtggt ccaaactact caacaagtac cttgatgtac
     5461 tacctttacg aaaaagcctt ccaattgaca gaatacggct atgccaacac aattggtgtc
     5521 ttcttggcag tcatgattgc tatcgtaagc tttgttcaat ttaaagtact tggaaacgac
     5581 gtagaatact aaagaaagga gacagctatg caatctacag aaaaaaaacc attaacagcc
     5641 tttactgtta tttcaacaat cattttgctc ttgttgactg tgctgttcat ctttccattc
     5701 tactggattt tgacaggggc attcaaatca caacctgata caattgttat tcctcctcag
     5761 tggttcccta aaatgccaac catggaaaac ttccaacaac tcatggtgca gaaccctgcc
     5821 ttgcaatgga tgtggaactc agtatttatc tcattggtaa ccatgttctt agtttgtgca
     5881 acctcatctc tagcaggtta tgtattggct aaaaaacgtt tctatggtca acgcattcta
     5941 tttgctatct ttatcgctgc tatggcgctt ccaaaacaag ttgtccttgt accattggta
     6001 cgtatcgtca acttcatggg aatccacgat actctctggg cagttatctt gcctttgatt
     6061 ggatggccat tcggtgtctt cctcatgaaa caatttagtg aaaatattcc tacagagttg
     6121 cttgaatcag ctaaaatcga cggttgtggt gagattcgta ccttctggag tgtagccttc
     6181 ccgattgtga aaccagggtt tgcagccctt gcaatcttta ccttcatcaa tacttggaat
     6241 gactacttca tgcaattggt aatgttgact tcacgtaaca atttgaccat ctcacttggg
     6301 gttgcgacca tgcaggctga aatggcaacc aactatggtt tgattatggc aggagctgcc
     6361 cttgctgctg ttccaatcgt cacagtcttc cttgtcttcc aaaaatcctt cacacagggt
     6421 attactatgg gagcggtcaa aggataatac tctgcgaaaa tcgaatgcaa actacgtcag
     6481 cttcaccttg ccatacttaa gtattgcctg cggttagctt cctagtttgt tcttcaattt
     6541 tcattgagta taggaaaatc aatctatcaa gatacagaag tatattttat agatttagag
     6601 aatatagagg ttataagtgt ctacaaaatg gagggtatgc agttacttta tgaagttttg
     6661 tcagacactt atcaacttaa gaatggtttt agttaactat cagaaacgaa ggaaagagta
     6721 tgatttttga cgatttgaaa aacatcacct tttacaaagg gattcatccc aatttagaca
     6781 aggctatcga ctacctctac caacatcgta aagattcatt cgaattagga aagtatgaga
     6841 ttgatggaga taaagtcttt ctagttgttc aggaaaatgt cctcaatcaa gttgagaata
     6901 atcaatttga acaccataag aactatgcag atttgcattt gctgatagaa gggcatgaat
     6961 attcgagcta cggttcacgt atcaaagacg aggcagtagc attcgacgaa gcgagtgaca
     7021 ttggctttgt tcattgtcat gaacactacc cgctcttgtt gggttatcac aattttgcga
     7081 ttttcttccc aggtgagcca catcagccaa atggttatgc aggcatggaa gaaaaggttc
     7141 gaaaatatct ctttaaaatc ttgattgatt aaaaatagga tgaattgttt ttttgtaaag
     7201 ctttgataat actctaccat gaaattgatc tttgtgaggt agagaaatga gaataaaata
     7261 tttaaaaatt ggtatcttct aagtatgctg caagagctag tttcttagat ggacagggga
     7321 ttacagttga tgagatgtcc tggataatta ggggcattgt gaatggattg attggtagat
     7381 acataaaatt aggtacttat gcagctaagt atggtattag tatggcgcgc tcgatcttaa
     7441 gtagggcagc tgcaactgcg gcagcaagag taagattatt gactaagatt tttggatgga
     7501 ttttacgaat agctgtgctc gcagctgatg tatatggtaa ttttgccaac aatattgctg
     7561 cagcttggga tgcacatgat aaaatcccta acaatggtcg tataaacttt taaaatgcga
     7621 gaatgaaagc actttgtatt tttttattgt atatgttagc ttggacagtg cttgcaatga
     7681 taatccgtgg agggctagat ggatttgatg aacacacttg gcgtaatatt ttagctagtg
     7741 cgttgtcctc gctatacata cattatatat ccatagatag aaaaagaaaa aagtccaaaa
     7801 gaaaaaagaa aaaatagatt tgttcatggt agggacttat gaaagcttta ctaacaaaaa
     7861 agaaaacagt ttacaaagaa aaatgatgga ggagcaaaca tggcacaaaa aggagtaagc
     7921 cttatcaagg cagcatttga tacagataac tttctcatgc gttttagtga gaaagtcttg
     7981 gacatcgtga cagccaatct tctttttgtc gtctcttgtt tgcccatcgt gacgattgga
     8041 gtggctaaaa tcagcctcta cgagaccatg ttcgaagtta agaagagcag acgggtgcct
     8101 gtttttaaaa tctatctaag atctttcaag caaaatctga aactaggtct tcagctgggt
     8161 ttaatggagt taggaattgt gtttcttacc ctttcagatc tctatctttt ctggggtcaa
     8221 acagctctgc ccttccaatt gctgaaagcc atttgtctag gcattctgat tttccttacg
     8281 attgtgatgt tggctagcta tccaatcgcg gcacgttatg atttaacttg gaaagaagtg
     8341 ttacaaaaag ggcttatctt ggctagtttt aactttcctt ggttcttcct catgttagcc
     8401 attcttgtcc tcattgtgat ggttctttat ctgtccgcct tcagtctact cttaggtggc
     8461 tcagtcttcc tactttttgg gtttggacta ttggtcttta tccagactgg attgatggag
     8521 aaaattttcg caaaatacca ataggagcct tatttctgaa actactttca aaggctccaa
     8581 acgctattct ataagcgaga aactaaaatc taagtgtcta taagatattg aaagcctttt
     8641 cggtaaagtg tatactaaag aagtaaagaa aaatctgctt tagcagaaaa taaaaataat
     8701 aggagaaaat ctatgtcaga tttgaaaaaa tacgaaggtg tcattccagc cttctacgca
     8761 tgttatgatg atcaaggaga agtaagccca gaacgtacgc gtgccttggt tcaatacttc
     8821 attgataaag gtgttcaagg tctttatgtc aatggttctt ctggtgaatg tatctaccaa
     8881 agcgttgaag atcgcaagtt gattttggaa gaagtcatgg cggtagccaa agataaattg
     8941 accattattg cccatgttgc ttgcaataat actaaagata gtatggaact tgctcgccat
     9001 gctgaaagct tgggagtaga tgctattgca acgattccac caatttattt ccgcttgcca
     9061 gaatactcag ttgccaaata ctggaacgat atcagttctg cagctccaaa cacagactac
     9121 gtgatttaca acattcctca attggcaggg gttgctttga ctccaagcct ttacacagaa
     9181 atgttgaaaa atcctcgtgt tatcggtgtg aagaactctt ctatgccagt tcaagatatc
     9241 caaacctttg tcagccttgg tggagaagac catatcgtct ttaatggtcc tgatgagcag
     9301 ttcctaggag gacgcctcat gggggctagg gctggtatcg gtggtactta tggtgctatg
     9361 ccagaactct tcttgaaact caatcagttg attgcggata aggacctaga aacagcgcgt
     9421 gaattgcagt atgctatcaa cgcaatcatt ggtaaactca cttctgctca tggaaatatg
     9481 tacggtgtca tcaaagaagt cttgaaaatc aatgaaggct tgaatattgg atctgttcgt
     9541 tcaccattga caccagtgac tgaagaagat cgtccagttg tagaagcggc tgctgccttg
     9601 attcgtgaaa ccaaggagcg cttcctctaa tctaaaagga ggtatttatg acatattacg
     9661 ttgcaattga tatcggtgga accaacatca agtatggttt ggttgatcaa gaggggcaac
     9721 ttcttgaatc gcatgaaatg ccaactgagg cgcataaggg tggacctcat atcttacaaa
     9781 agaccaaaga tatcgtagct agttatttag aaaaaggccc agtagcaggt gttgccatat
     9841 cttctgctgg gatggtggat ccggataagg gtgagatttt ctatgctggg ccgcaaatcc
     9901 ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga aagctttact attccttgtg
     9961 agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt tcaggcaagg
    10021 gagcaagtgt gacactttgc ttgaccattg gaacaggtat cggtggttgc ttgattatgg
    10081 ataggaaagt cttccatggt tttagcaatt cagcctgtga agtcgggtat atgcatatgc
    10141 aggatggagc ttttcaggat ctcgcttcta caacagcttt agtgaaatat gtagctgaag
    10201 cccatggaga agatgttgat cagtggaatg gccgacgtat ctttaaggaa gctactgaag
    10261 gaaacaaaat ctgcatggaa ggcattgacc gtatggttga ctatctagga aaaggtctgg
    10321 caaatatttg ctacgttgcc aatccagaag tggttattct tggtggtggt atcatggggc
    10381 aagaggctat cctcaaacct aagatccgca cagccttgaa agaggctttg gtaccaagtt
    10441 tagcagaaaa aacacgatta gaatttgccc atcaccaaaa tacagcaggg atgttgggtg
    10501 catattatca ttttaagaca aaacaatcct agtttggctc agccaaacta ggattttctt
    10561 acacgttttt gtctacgata gccgttgagt tttttatttt cccagtagct attaaagatt
    10621 ttttccttgc tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc gataaagaag
    10681 agcataggaa gttgagcgga tattcgttgg atataggagg gttggctgtg ggtggctaca
    10741 agaacagtct ctgtataggt ctggctatct ttattgggaa cacttgtaaa gagtacagtc
    10801 tttgccccca tctccttggc atctaataga ctatctaaaa tagaaggagt tgagcctgaa
    10861 agtgagaagc ccagtactag acaatttcca tccatgatgc tggttgtcca ggcaaagccg
    10921 tcttggtctg tcaaagcttc gcagaccaca cctagtcgca taaaacgtaa tttcatttca
    10981 cgggcgacga ggccagaact ccctgttcca aagaagtaga tacgctcagc atcttcgatt
    11041 agctgggcaa ttcgctctag ttggatttcg tcaatcaagt cttgtgtttg ttccctcata
    11101 ttgctataac ttctgaggac tcgtttggtc agtggactgt gcttggagac ttggttggct
    11161 tgattttctg cctgatgttg gtattggaaa ataaattctc ggtagccagt aaagccacac
    11221 tttttagcaa agcgggtcaa agcagcttga gaaatatgta atttttgggt gacttgttga
    11281 gaagataaat catctgtaat cgtttcagct tgcaaaaaat agcgagcgat ttcttgctct
    11341 aggtctgtca tttcttcaaa atgtgaatca atgatagttg cgatatctgg tttgtccata
    11401 gggaaagctc ctttacatga gtcatactgg aagactagat cagggaatag tcacacttca
    11461 ttataacaca taatataagg atagataaat aaaaacgcat ttctgtttta aaaacgaaaa
    11521 aatcgaaaaa gcttctttct tttccataat tttctactca aattgtggta caattaagag
    11581 taagatttta agttagaaat gagactgatt tgtatgagaa aatttaacag ccattcgatt
    11641 ccgattcggc ttaatttatt gttttcaatc gtcattttac tctttatgac cattattggt
    11701 cgtttgttgt atatgcaggt tttgaacaag gatttttacg aaaaaaagct agcctcagct
    11761 agtcagacca agattacaag cagttcagcc cgtggggaaa tttatgatgc tagtggaaaa
    11821 cctttggtag aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa taaaatgacg
    11881 gctacagact taaaagaaac agctaaaaag ttactgactt atgtgagcat cagttctcca
    11941 aatttgacag aacgccagct ggcggattac tatttggctg atcctgaaat ctataaaaaa
    12001 acagtggaag ctctcccaag tgagaaacgc ttggattcag atggcaatcg tctatccgaa
    12061 tcagaactgt ataacaatgc ggtcgatagt gtccaaacaa gtcaactaaa ctatacagag
    12121 gatgaaaaga aagaaatcta tctttttagt cagttaaatg ctgttggaaa ctttgcgaca
    12181 ggaaccattg ctacagatcc tctaaatgat tctcaggtgg ctgttattgc ctctatttca
    12241 aaggagatgc ctggcattag tatttctact tcttgggata gaaaggtttt ggaaacttcc
    12301 ctttcttcta tagttgggag tgtatccagt gaaaaagctg gtctcccagc ggaagaagca
    12361 gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg taggaacctc ctatttggaa
    12421 aagcaatatg aagagacctt acaaggaaaa cgctcggtaa aagaaatcca tctggataaa
    12481 tatggcaata tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa caatatcaaa
    12541 ctgaccattg atttggcttt ccaagatagc gtggatgctt tactgaaaag ttatttcaat
    12601 tccgagctag aaaatggtgg agccaagtat tctgaaggtg tctatgcagt cgcccttaac
    12661 ccaaaaacag gtgcggtttt gtctatgtca gggattaaac atgacttgaa aacgggagag
    12721 ttgacgcctg attccttggg aacggtaacc aatgtctttg ttccaggttc ggttgtcaag
    12781 gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt caggaaacca gaccttgaca
    12841 gaccagtcca ttgtcttcca aggttcagct cccatcaatt cttggtatac tcaggcttac
    12901 ggttcattcc ctatcacagc ggtccaagct ctggagtatt catcaaatac ctatatggtc
    12961 caaacagcct taggtcttat ggggcaaacc tatcaaccca atatgtttgt cggcaccagc
    13021 aatctagagt ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg cttgggtact
    13081 gcgacaggaa ttgacctacc agatgaatct actggatttg ttcccaaaga gtatagcttt
    13141 gctaattaca ttactaatgc ctttgggcag tttgataact atacgccgat gcagttggct
    13201 cagtatgtag caactattgc aaataatggt gttcgtgtgg ctcctcgtat tgttgaaggc
    13261 atttatggta ataatgataa gggaggactg ggtgacttga ttcagcaact gcaaccgaca
    13321 gagataaata aggtcaatat atccgactcc gatatgagca tcttgcacca aggtttttat
    13381 caggttgccc atggtactag tggattgaca actggacgtg ccttttcaaa tggcgccttg
    13441 gtatccatta gcggaaaaac aggtacagcc gaaagctatg tggcagatgg tcagcaagca
    13501 accaatacca atgcggtggc ctatgcccca tctgataatc cccaaatcgc tgttgcagtg
    13561 gtctttcctc ataataccaa tctaacaaat ggtgtaggac cttccattgc gcgtgacatt
    13621 atcaatctgt atcaaaaata ccatccaatg aactagaaag gaaattatgc tttatccaac
    13681 acctattgcc aagttgattg acagttattc taagttacca ggtatcggga ttaagacggc
    13741 tacgcgtctg gccttttata cgattgggat gtctgctgat gatgtcaatg aatttgcaaa
    13801 aaatctcctt tctgctaaga gagaattgac atattgttct atttgtggac gtttgacaga
    13861 cgacgatcct tgttctatct gtactgatcc gactcgtgac cagacaacaa ttttagttct
    13921 tgaggatagt agagatgtgg cagccatgga aaatatccaa gaataccatg gactctatca
    13981 tgtccttcat ggcctcattt ctcctatgaa tggtatcagt ccggacgata tcaatctcaa
    14041 gagccttatg actcgtctta tggatagtga agtttcagaa gtgattgtgg cgactaatgc
    14101 tacagcggat ggtgaagcga cttccatgta tctttcacgt ttgctcaagc cggctggtat
    14161 caaggttacg cgtctagcac gaggtctcgc tgtgggagcg gacattgagt atgcggacga
    14221 agtgacactc ttacgagcga ttgaaaatcg gacagagttg taagtgtagg caaatttacg
    14281 aactccattc atttataaaa aatcaaagag gctgaaaatc gttcctatcg gcctcttttt
    14341 gtatagtgtg atgagtagga tcaggttcaa gttttaaaaa accaagcaaa tatgatatac
    14401 taaagagcga gtattctagt agaattagga caaataatat gaaacaaacg attattcttt
    14461 tatatggtgg acggagtgcg gaacgcgaag tctctgtcct ttcagctgag agtgtcatgc
    14521 gtgcggtcaa ttacgaccgt ttcacagtca agactttctt tatcagtcag tcaggtgact
    14581 ttatcaaaac acaggaattt agtcatgctc cggggcaaga agaccgtctc atgaccaatg
    14641 aaaccattga ttgggataag aaagttgcac caagtgctat ctacgaagaa ggtgcagtgg
    14701 tctttccagt ccttcacggg ccaatgggag aagatggctc tgttcaagga ttcttggaag
    14761 ttttgaaaat gccttacgtt ggttgcaaca ttttgtcatc aagtcttgcc atggataaaa
    14821 tcacgactaa gcgtgttctg gaatctgctg gtattgccca agttccttat gtggctatcg
    14881 ttgaaggcga tgatgtgact gctaaaatcg ctgaagtgga agaaaaattg gcttatccag
    14941 tcttcactaa gccgtcaaac atggggtcta gtgtcggtat ttctaagtct gaaaaccaag
    15001 aagaactccg tcaagcctta aaacttgcct tccgatatga cagccgtgtc ttggttgagc
    15061 aaggagtgaa tgcccgtgaa attgaggttg gcctcttggg taactacgat gtcaagagca
    15121 cgctacctgg agaagttgtc aaggacgttg ccttttatga ctacgatgcc aagtatattg
    15181 ataacaagat tactatggat attcctgcca aaatcagtga tgatgtggtg gctgtcatgc
    15241 gtcaaaatgc agaaacagcc ttccgtgcca ttggtggcct tggtctatct cgttgcgatt
    15301 tcttctatac agataaggga gagatttttc tcaacgagct caataccatg ccaggtttta
    15361 cccagtggtc tatgtaccca ctactttggg acaatatggg gatcagctac ccagaactaa
    15421 tcgagcgttt ggttgacctt gccaaggaaa gttttgacaa gcgcgaagcg catttgatat
    15481 aaaatgaaag agagggtaga agccagaacc atcactgcac ggtgactaga gttctcggac
    15541 ttcagccctt tttaaaggag tagaaatgaa attaacaatc catgaaattg cccaagttgt
    15601 tggagccaaa aatgatatca gtatctttga ggacacccag ttagaaaaag ctgagtttga
    15661 tagtcgtttg attggaactg gagatttatt tgtgccactt aaaggtgcgc gtgatggcca
    15721 tgactttatt gaaacagcct ttgaaaatgg tgcagcagta accttgtctg agaaagaggt
    15781 ctcaaatcat ccttacattc tagtagatga tgttttgaca gcctttcaat ccctagcatc
    15841 ctactatctt gaaaaaacga ctgttgatgt ctttgctgtt acaggttcaa atggcaagac
    15901 aacgactaag gatatgttgg cgtatttact gtcaacaaga tacaagacct acaaaacaca
    15961 aggcaattac aataatgaga ttggccttcc ttacacagtt ctccacatgc ctgaaggaac
    16021 agaaaagttg gtcttggaga tggggcagga ccacttggga gatattcatc tcttgtctga
    16081 attggctcgt ccaaaaacag ccatcgtgac cttggttgga gaagctcatt tggccttttt
    16141 caaagatcgt tctgagattg ctaaaggaaa aatgcaaatt gcagacggaa tggcttcagg
    16201 ttccttgctt ttagcgccgg ctgaccctat cgtagaggac tatttgccaa ctgataaaaa
    16261 ggtggttcgt tttggtcaag gagcagagct ggaaattact gacttggttg agcgcaaaga
    16321 tagtctgacc ttcaaggcca atttcttaga gcaagtcctt gatttgccag taactggcaa
    16381 gtataatgcg acaaatgcta tgattgcatc ctatgttgcc ttgcaagaag gagtttcaga
    16441 ggagcaaatt catcaggcct tccaagatct tgaattgacg cgtaaccgta ccgaatggaa
    16501 gaaagcagcc aatggagcag atatcctgtc tgatgtatac aatgccaatc caactgctat
    16561 gaagttgatt ttagagactt tctctgccat tccagccaat gaaggtggca agaaaattgc
    16621 ggttttagcg gacatgaagg aactgggtaa ccagtcagtt caacttcata atcagatgat
    16681 tttgagcctt tctccagatg tgcttgatac cgtgattttc tatggagaag acattgctga
    16741 attagcccaa ttggccagtc aaatgttccc aattggccac gtttactact tcaagaaaac
    16801 agaagaccaa gaccaatttg aagacctagt caagcaggtc aaggaaagcc tcagtgccaa
    16861 tgatcaaatc ttgctcaagg gctctaactc tatgaaccta gctatgttgg tagaaagttt
    16921 agaaaatgaa accaagtgat tttgttaagt atctgcaaag aatgattgcc cttacagata
    16981 ctggcttaac ctttacaaaa gatccttttg accgtgagcg ctacgaggac ttgcgaagtc
    17041 tgttatctga aatgttgaat cagggatcag acctggatgc agaagaagta gcagaagtct
    17101 tgaaaccaac ttcagcttat gcgactccct taatggacgt ccgtgcttgg attgttgagg
    17161 atgagaagat ttgtctggtt aggggacaag gagagggtag ttgggctttg ccaggtggct
    17221 ttggtgaagt cggctattct ccaactgaaa atattctcaa ggaaattgaa gaagaaaccg
    17281 gttttaaagc caaagttgaa agactgctag ctgtttttga tatcaatcgt ttccaactac
    17341 agagcaaaca atatgcaaag tttgtttttg aatgtaagct tctcgatgga caattccaag
    17401 aaaatcaaga aattgcagac cttcaatttt ttgccattga tcaactgcca gtcttatctg
    17461 aaaaacgcat taccaaggag caaatagaga ttctttggca ggtttatcaa ggtcagaggg
    17521 agcaatatct tgactaagaa gatgattatc gtatttttaa atccattttt aacaactagc
    17581 atggtataat aatatgcagg aaaattttga attatgagga agactagatg aatttatggg
    17641 atattttctt tacgactcag gcaaccaagc cgcccaaatt tgaccttttt tggtatgtta
    17701 gcctatttac gctcttagcc ttaacctttt atacagccca tcgctatcgt gaaaagaagg
    17761 tttaccaacg atttttccaa atcttgcaga ctgttcagtt aatccttctt tatggttggt
    17821 actgggtcaa tcatatgcca ctgtcagaaa gcctaccctt ttaccattgc cgtatggcta
    17881 tgtttgtggt actcttgctt cctggtcaat ccaaatataa acaatacttt gcattattgg
    17941 gaacatttgg gacattagca gcctttgttt atccagtgcc agatgcttac ccttttccac
    18001 atatcaccat tctatccttt atctttggtc atttagcact cttggggaac tctctagttt
    18061 atctattgag acagtataat gcgcgattgc tggatgtgaa gggaattttt ctcatgacct
    18121 ttgccctaaa tgccttgatt tttgtggtca atttggtgac aggtggcgat tacggatttt
    18181 tgacaaaacc gccattggtt ggggatcacg gtctagtagc taattattta cttgtttcaa
    18241 ttgtgctggt agctactatc agtttgacta agaaaatctt agaattcttt ttagctcaag
    18301 aagcagaaaa aatgattgta aaggaagctt aacacagagc tttctttttt gctcttagag
    18361 agtttttaca atcagcttat aaaataagaa tttctgaata gacaaactca aaaaatggct
    18421 gggaaattta ggaaaaaagc aagcacgatt aaattttttg tgttataata ttttgtgaat
    18481 agctatgcct atgtttagct atggaataat acgaagtgcg aaacttggaa gatagagagg
    18541 aagcgatgta atggctagag aaggcttttt tacaggtcta gatattggaa caagctctgt
    18601 caaggtgctt gtggccgagc agagaaatgg tgaattaaat gtaattggcg tgagtaatgc
    18661 caaaagtaaa ggtgtaaagg atggaattat tgttgatatt gatgcagcag caactgctat
    18721 caagtcagcc atttcccaag cggaagaaaa ggcaggcatt tcgattaaat cagtaaatgt
    18781 cggcttgcct ggtaatcttt tgcaggtaga accaactcag gggatgattc cagtaacatc
    18841 tgatactaag gaaattacgg atcaagatgt tgaaaatgtt gtcaaatcag ctttgacaaa
    18901 gagtatgaca cctgaccgtg aagtcattac ctttattcct gaagaattta ttgtggatgg
    18961 tttccaaggg attcgtgacc cacgtggcat gatgggggtt cgccttgaaa tgcgtggttt
    19021 gctttataca ggacctcgta ctatcttgca caatttgcgt aagacggttg agcgtgcagg
    19081 tgttcaggtt gaaaatgtta tcatttcacc actagcaatg gttcagtctg ttttgaacga
    19141 aggggaacgt gaatttggtg ctacagtgat tgatatgggg gcaggtcaaa cgactgtcgc
    19201 tacaatccgt aatcaagaac tccagttcac acatattctc caagaaggtg gagattatgt
    19261 aactaaagat atctccaagg ttttgaaaac ctctcgcaaa ttagcggaag gcttgaaact
    19321 gaattacggg gaagcctatc cgcctcttgc aagcaaagaa accttccaag tagaggttat
    19381 tggagaagta gaagcagtcg aagtgacgga agcctacttg tcagaaatta tttctgcacg
    19441 aatcaagcac atccttgaac aaatcaagca agaattagat agaaggcgtc tattggacct
    19501 ccctggtggt attgtcttaa tcggtgggaa tgccatttta ccaggtatgg ttgagcttgc
    19561 tcaggaagtc tttggcgtcc gtgtcaagct ttatgttcca aatcaagttg gtatccgtaa
    19621 tccagccttt gcgcatgtga ttagtttatc agaatttgcg ggtcaattaa cagaagttaa
    19681 tcttttggct cagggagcga taaaaggtga gaatgactta agtcatcagc caattagttt
    19741 tggtgggatg ctgcaaaaaa cagctcagtt tgtacaatca acgcctgttc aaccagctcc
    19801 tgctccagaa gtagagccgg tggcgcctac agaaccaatg gcggatttcc aacaagcttc
    19861 acaaaataaa ccgaaattag cagatcgttt ccgtggcttg atcggaagca tgtttgacga
    19921 ataaagagga aaaataaatt atgacatttt catttgatac agctgctgct caaggggcag
    19981 tgattaaagt aattggtgtc ggtggaggtg gtggcaatgc catcaaccgt atggtcgacg
    20041 aaggtgttac aggcgtagaa tttatcgcag caaacacaga tgtacaagca ttgagtagta
    20101 caaaagctga gactgttatt cagttgggac ctaaattgac tcgtggtttg ggtgcaggag
    20161 gtcaacctga ggttggtcgt aaagccgctg aagaaagcga agaaacactg acggaagcta
    20221 ttagtggtgc cgatatggtc ttcatcactg ctggtatggg aggaggctct ggaactggag
    20281 ctgctcctgt tattgctcgt attgccaaag atttaggtgc gcttacagtt ggtgttgtaa
    20341 cacgtccctt tggttttgaa ggaagtaagc gtggacaatt tgctgtagaa ggaatcaatc
    20401 aacttcgtga gcatgtagac actctattga ttatctcaaa caacaatttg cttgaaattg
    20461 ttgataagaa aacaccgctt ttggaggctc ttagcgaagc ggataacgtt cttcgtcaag
    20521 gtgttcaagg gattaccgat ttgattacca atccaggatt gattaacctt gactttgccg
    20581 atgtgaaaac ggtaatggca aacaaaggga atgctcttat gggtattggt atcggtagtg
    20641 gagaagaacg tgtggtagaa gcggcacgta aggcaatcta ttcaccactt cttgaaacaa
    20701 ctattgacgg tgctgaggat gttatcgtca acgttactgg tggtcttgac ttaaccttga
    20761 ttgaggcaga agaggcttca caaattgtga accaggcagc aggtcaagga gtgaacatct
    20821 ggctcggtac ttcaattgat gaaagtatgc gtgatgaaat tcgtgtaaca gttgttgcaa
    20881 cgggtgttcg tcaagaccgc gtagaaaagg ttgtggctcc acaagctaga tctgctacta
    20941 actaccgtga gacagtgaaa ccagctcatt cacatggctt tgatcgtcat tttgatatgg
    21001 cagaaacagc tgaattgcca aaacaaaatc cacgtcgttt ggaaccaact caggcatctg
    21061 cttttggtga ttgggatctt cgccgtgaat cgattgttcg tacaacagat tcagtcgttt
    21121 ctccagtcga gcgctttgaa gccccaattt cacaagatga agatgaattg gatacacctc
    21181 catttttcaa aaatcgttaa gtaaatgaat gtaaaagaaa atacagaact tgtttttcga
    21241 gaagttgcag aggctagtct gagtgctcat cgagagagtg gttcggtctc tgtcattgca
    21301 gttaccaagt atgtagatgt accgacagcg gaagccttgc ttccgctagg tgttcatcat
    21361 atcggtgaaa atcgtgtaga taagtttctg gaaaaatatg aagctttaaa agatcgagat
    21421 gtgacttggc atttgattgg taccttgcaa agacgtaagg tgaaagatgt cattcaatac
    21481 gttgattatt tccatgcatt ggactcagta aagctagcag gggaaattca aaaaagaagt
    21541 gaccgagtca tcaagtgttt ccttcaagta aatatttcta aagaagaaag caaacacggt
    21601 ttttcgagag aggaactgct ggaaatcttg ccagagttag ccagactaga taagattgaa
    21661 tatattggtt taatgacgat ggcacctttt gaggctagca gtgagcagtt gaaagagatt
    21721 ttcaaggcgg cccaagattt acaaagagaa attcaagaga aacaaattcc aaatatgcct
    21781 atgaccgagt taagtatggg aatgagtcgt gattataaag aagcgattca attcggttcc
    21841 acttttgttc gtataggtac atcatttttt aagtaggaga gaaccatgtc tttaaaagat
    21901 agattcgata gatttataga ttattttacg gaggatgagg attcaagtct cccttatgaa
    21961 aaaagagatg agcctgtgtt tacttcagta aattcttcac aggaaccggc tctcccaatg
    22021 aatcaacctt cacagtcggc tggtacaaaa gagaacaata tcaccagact tcatgcaaga
    22081 caacaggaat tggcaaatca gagtcagcgt gcaacggata aggtcattat agatgttcgt
    22141 tatcctagaa aatatgagga tgcaacagaa attgttgatt tattggcagg aaacgaaagt
    22201 atcttgattg actttcagta tatgacagag gtgcaggctc gtcgttgttt ggactatttg
    22261 gatggagctt gtcatgtttt agctggaaat ttgaaaaagg tagcttctac catgtatttg
    22321 ttgacaccag tgaacgttat tgtaaatgtt gaagatatcc gtttaccaga tgaagatcaa
    22381 cagggtgagt tcggttttga tatgaagcga aatagagtac gataatgatt tttttaattc
    22441 gtatgattta taatgcagtg gatatttact ccctgatttt ggtagccttc gctgtcatgt
    22501 cttggtttcc aggtgcctac gaatccagtt taggtcgttg gattgtagcg ttggtgaaac
    22561 cagtgcttgc tcccttgcaa cgcctgcctt tacagatagc gggtcttgat ttatctgttt
    22621 gggttgcgat tgttttggtt cgatttttag gagaaaacct agtgcgtttt ctggcgatga
    22681 taggatgaat aaagggattt atcagcattt ctccatagaa gatcgtccat ttcttgacaa
    22741 gggaatggaa tggataaaga aggtagaaga tagctatgct ccttttttaa ctccttttat
    22801 caatcctcat caggagaagc tattaaagat tttggccaaa acctatggtc ttgcttgtag
    22861 cagtagtggg gaattcgtct cgagtgagta tgttcgagtt ttattatacc cagattattt
    22921 ccaaccagag ttttcagatt ttgaaatatc tctccaggaa attgtgtatt ccaataaatt
    22981 tgaacattta acgcatgcta agattttagg gacagtcatc aatcaattag ggattgaacg
    23041 gaaacttttt ggagatatcc tagtagatga agaacgggcg cagattatga ttaatcagca
    23101 gtttcttctt ctctttcaag atggactaaa gaaaattggt cgtatacctg tttcgctgga
    23161 ggaacgtcct ttcaccgaga aaatagataa gctagaacag tatcgagaac tggatttatc
    23221 tgtgtctagt tttcgattag atgttctttt atcaaatgtt ttgaaactat ctaggaatca
    23281 agcaaaccag ttgattgaaa agaaacttgt ccaagtaaat tatcatgtgg tagacaaatc
    23341 agattacact gttcaagttg gagacttgat tagtgtgaga aaatttggtc gcttgagatt
    23401 acttcaagat aagggacaaa cgaaaaaaga gaagaaaaaa ataaccgtcc agttattatt
    23461 aagtaagtga ggaatagaat gccaattaca tcattagaaa taaaggacaa gacttttgga
    23521 actcgattca gaggttttga tccagaagaa gtcgatgaat ttttagatat tgtggttcgt
    23581 gattacgaag atcttgtgcg tgcgaatcat gataaaaatt tgcgtattaa gagtttagaa
    23641 gagcgtttgt cttactttga tgaaataaaa gattcattga gccagtctgt attgattgct
    23701 caggatacag ctgagagagt gaaacaggcg gcgcatgaac gttcaaacaa tatcattcat
    23761 caagcagagc aagatgcgca acgcttgttg gaagaagcta aatataaggc aaacgagatt
    23821 cttcgtcaag caactgataa tgctaagaaa gtcgctgttg aaacagaaga attgaagaac
    23881 aagagccgtg tcttccacca acgtctcaaa tctacaattg agagtcagtt ggctattgtt
    23941 gaatcttcag attgggaaga tattctccgt ccaacagcta cttatcttca aaccagtgat
    24001 gaagccttta aagaagtggt tagcgaagta cttggagaac cgattccagc tccaattgaa
    24061 gaagaaccaa ttgatatgac acgtcagttc tctcaagcag aaatggcaga attacaagct
    24121 cgtattgagg tagccgataa agaattgtct gaatttgaag ctcagattaa acaggaagtg
    24181 gaagctccaa ctcctgtagt gagtcctcaa gttgaagaag agcctctgct catccagttg
    24241 gcccaatgta tgaagaacca gaagtagctc caatgcatcc gataggtcca acaccagcta
    24301 cagaaactgt tgattcaata ccgggatttg aagcaccgca agaatctgtt acaattttat
    24361 aagaaatatt ctgagaacaa tatcttatcc ttatatttcc agcgagcagg agatggtgtg
    24421 agtcctgtaa tccctattga taagattatc ctctcaaaaa ctcaagtctg aagctagtaa
    24481 gatttgacgt ttcccacgtt acgggataag agggagaaag actaaatctt tttccgaata
    24541 aaggtggtac cacgattttc gtcctttttg gaagtcgtgg tttttaattt gttattattt
    24601 ataaaggaga taccatgaaa ctcaaagaca cccttaatct tgggaaaact gaattcccaa
    24661 tgcgtgcagg ccttcctacc aaagagccag tttggcaaaa ggaatgggaa gatgcaaaac
    24721 tttatcaacg tcgtcaagaa ttgaaccaag gaaaacctca tttcaccttg catgatggcc
    24781 ctccatacgc taacggaaat atccacgttg gacatgctat gaacaagatt tcaaaagata
    24841 tcattgttcg ttctaagtct atgtcaggat tttacgcacc atttattcct ggttgggata
    24901 ctcatggtct gccaatcgag caagtcttgt caaaacaagg tgtcaaacgt aaagaaatgg
    24961 acttggttga gtacttgaaa ctttgccgtg agtacgctct ttctcaagta gataaacaac
    25021 gtgaagattt taaacgtttg ggtgtttctg gtgactggga aaatccatat gtgaccttga
    25081 ctcctgacta tgaagcagct caaattcgtg tatttggtga gatggctaat aagggttata
    25141 tctaccgtgg tgctaagcca gtttactggt catggtcatc tgagtcagca cttgctgaag
    25201 cagagattga ataccatgac ttggtttcaa cttcccttta ctatgccaac aaggtaaaag
    25261 atggcaaagg agttctagat acagatactt atatcgttgt ctggacaacg actccattta
    25321 ccatcacagc ttctcgtggt ttgacggttg gtgcagatat tgattacgtt ttggttcaac
    25381 ctgctggtga agctcgtaag tttgtcgttg ctgctgaatt attgactagc ttgtctgaga
    25441 aatttggctg ggctgatgtt caagttttgg aaacttaccg tggccaagaa ctcaaccaca
    25501 tcgtaacaga acacccatgg gatacagctg tagaagagtt ggtaattctt ggtgaccacg
    25561 ttacgactga ctctggtaca ggtattgtcc atacagcccc tggttttggt gaggacgatt
    25621 acaatgttgg tattgctaat aatcttgaag tcgcagtgac tgttgatgaa cgtggtatca
    25681 tgatgaagaa tgctggtcct gaatttgaag gtcaattcta tgaaaaggta gttccaactg
    25741 ttattgaaaa acttggtaac ctccttcttg cccaagaaga aatctctcac tcatatccat
    25801 ttgactggcg tactaagaaa ccaatcatct ggcgtgcagt tccacaatgg tttgcctcag
    25861 tttctaaatt ccgtcaagaa atcttggacg aaattgaaaa agtgaaattc cactcagaat
    25921 ggggtaaagt ccgtctttac aatatgatcc gtgaccgtgg tgactgggtt atctctcgtc
    25981 aacgtgcttg gggtgttcca cttcctatct tctacgctga agatggtaca gctatcatgg
    26041 tagctgaaac tattgaacac gtagctcaac tttttgaaga atatggttca agcatttggt
    26101 gggaacgtga tgccaaagac ctcttgccag aaggatttac tcatccaggt tcaccaaacg
    26161 gcgagttcaa aaaagaaact gatatcatgg acgtttggtt tgactcaggt tcatcatgga
    26221 atggagtggt ggtaaaccgt cctgaattga cttacccagc cgacctttac ctagaaggtt
    26281 ctgaccaata ccgtggttgg tttaactcat cacttatcac atctgttgcc aaccatggcg
    26341 tagcacctta caaacaaatc ttgtcacaag gttttgccct tgatggtaaa ggtgagaaga
    26401 tgtctaaatc tcttggaaat actattgctc caagcgatgt tgaaaaacaa ttcggtgctg
    26461 aaatcttgcg tctctgggta acaagtgttg actcaagcaa tgacgtgcgt atctctatgg
    26521 atatcttgag ccaagtttct gaaacttacc gtaagattcg taacactctt cgtttcttga
    26581 ttgccaatac atctgacttt aacccagctc aagatacagt cgcttacgat gagcttcgtt
    26641 cagttgataa gtacatgacg attcgcttta accagcttgt caagaccatt cgtgatgcct
    26701 atgcagactt tgaattcttg acgatctaca aggccttggt gaactttatc aacgttgact
    26761 tgtcagcctt ctaccttgat tttgccaaag atgttgttta cattgaaggt gccaaatcac
    26821 tggaacgccg tcaaatgcag actgtcttct atgacattct tgtcaaaatc accaaactct
    26881 tgacaccaat ccttcctcac actgcggaag aaatctggtc atatcttgag tttgaaacag
    26941 aagacttcgt ccaattgtca gaattaccag aagttcaaac ttttgctaac caagaagaaa
    27001 tcttggatac atgggcagcc ttcatggact ttcgtggaca agcacaaaaa gccttggaag
    27061 aagctcgtaa tgcaaaagtt atcggtaaat cacttgaagc acacttgaca gtttatccaa
    27121 atgaagttgt gaaaactcta ctcgaagcag taaacagcaa tgtagcacaa cttttgatcg
    27181 tgtctgagtt gaccatcgca gaaggaccag ctccggaagc tgcccttagc ttcgaagatg
    27241 tagccttcac agttgaacgt gctactggtg aagtatgtga ccgttgccgt cgtatcgacc
    27301 caacaacagc agaacgcagc taccaggcag ttatctgtga ccactgtgca agcatcgtag
    27361 aagaaaactt tgcggaagca gtcgcagaag gatttgaaga gaaataagat tgaaaagtct
    27421 aggcaaaatt caatttgaga agaaaagaca actaatttta tagtctatta aacgcattgt
    27481 atcacgtttt tgaatacctg atatgatgcg ttttttattt attttaaaaa tttgcgaggt
    27541 atgacttttt atactcaaca agaatcaaag agaaacttag caagctaaca gtagtaagat
    27601 aaaataggaa tttgatatta gggataagat tgg
//
LOCUS       DBERCA010000019        26384 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.019, whole genome shotgun sequence.
ACCESSION   DBERCA010000019 DBERCA010000000
VERSION     DBERCA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.019"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..990
                     /gene="lytB"
                     /locus_tag="ACL719_000993"
     CDS             <1..990
                     /gene="lytB"
                     /locus_tag="ACL719_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HGS0698985.1"
                     /translation="LKSDGKIAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1051..2706)
                     /gene="pavA"
                     /locus_tag="ACL719_000994"
     CDS             complement(1051..2706)
                     /gene="pavA"
                     /locus_tag="ACL719_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGS0698986.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKITSMKKS"
     gene            2811..3308
                     /gene="ybeY"
                     /locus_tag="ACL719_000995"
     CDS             2811..3308
                     /gene="ybeY"
                     /locus_tag="ACL719_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGS0698987.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3289..3684
                     /locus_tag="ACL719_000996"
     CDS             3289..3684
                     /locus_tag="ACL719_000996"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS0698988.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3701..4600
                     /gene="era"
                     /locus_tag="ACL719_000997"
     CDS             3701..4600
                     /gene="era"
                     /locus_tag="ACL719_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGS0698989.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4649..5473
                     /gene="mutM"
                     /locus_tag="ACL719_000998"
     CDS             4649..5473
                     /gene="mutM"
                     /locus_tag="ACL719_000998"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGS0698990.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5473..6078
                     /gene="coaE"
                     /locus_tag="ACL719_000999"
     CDS             5473..6078
                     /gene="coaE"
                     /locus_tag="ACL719_000999"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGS0698991.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            6065..7264
                     /locus_tag="ACL719_001000"
     CDS             6065..7264
                     /locus_tag="ACL719_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HGS0698992.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7264..7413
                     /gene="rpmG"
                     /locus_tag="ACL719_001001"
     CDS             7264..7413
                     /gene="rpmG"
                     /locus_tag="ACL719_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0698993.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7453..7686
                     /gene="secG"
                     /locus_tag="ACL719_001002"
     CDS             7453..7686
                     /gene="secG"
                     /locus_tag="ACL719_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGS0698994.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7788..10142
                     /gene="rnr"
                     /locus_tag="ACL719_001003"
     CDS             7788..10142
                     /gene="rnr"
                     /locus_tag="ACL719_001003"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGS0698995.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQTLGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDTVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10105..10572
                     /gene="smpB"
                     /locus_tag="ACL719_001004"
     CDS             10105..10572
                     /gene="smpB"
                     /locus_tag="ACL719_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGS0698996.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10588..11448
                     /gene="tehB"
                     /locus_tag="ACL719_001005"
     CDS             10588..11448
                     /gene="tehB"
                     /locus_tag="ACL719_001005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGS0698997.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11521..12474
                     /gene="coiA"
                     /locus_tag="ACL719_001006"
     CDS             11521..12474
                     /gene="coiA"
                     /locus_tag="ACL719_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGS0698998.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12493..14295
                     /gene="pepF"
                     /locus_tag="ACL719_001007"
     CDS             12493..14295
                     /gene="pepF"
                     /locus_tag="ACL719_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS0698999.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14297..15010
                     /locus_tag="ACL719_001008"
     CDS             14297..15010
                     /locus_tag="ACL719_001008"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGS0699000.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQRLFDATLDNSELTATLVPLG
                     DGILMLRKNVADVQLPDSE"
     gene            15077..16018
                     /gene="prsA"
                     /locus_tag="ACL719_001009"
     CDS             15077..16018
                     /gene="prsA"
                     /locus_tag="ACL719_001009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGS0699001.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16137..16478)
                     /locus_tag="ACL719_001010"
                     /pseudo
     CDS             complement(16137..16478)
                     /locus_tag="ACL719_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16892..16987
                     /locus_tag="ACL719_001011"
                     /pseudo
     CDS             16892..16987
                     /locus_tag="ACL719_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(17240..17860)
                     /locus_tag="ACL719_001012"
     CDS             complement(17240..17860)
                     /locus_tag="ACL719_001012"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0699002.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(17860..18342)
                     /locus_tag="ACL719_001013"
     CDS             complement(17860..18342)
                     /locus_tag="ACL719_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGS0699003.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(18342..19184)
                     /locus_tag="ACL719_001014"
     CDS             complement(18342..19184)
                     /locus_tag="ACL719_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGS0699004.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSH
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(19279..20079)
                     /locus_tag="ACL719_001015"
     CDS             complement(19279..20079)
                     /locus_tag="ACL719_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGS0699005.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            20223..21602
                     /gene="glmU"
                     /locus_tag="ACL719_001016"
     CDS             20223..21602
                     /gene="glmU"
                     /locus_tag="ACL719_001016"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGS0699006.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            21612..22157
                     /locus_tag="ACL719_001017"
     CDS             21612..22157
                     /locus_tag="ACL719_001017"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0699007.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            22169..22483
                     /gene="macP"
                     /locus_tag="ACL719_001018"
     CDS             22169..22483
                     /gene="macP"
                     /locus_tag="ACL719_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGS0699008.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            22500..23192
                     /locus_tag="ACL719_001019"
     CDS             22500..23192
                     /locus_tag="ACL719_001019"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGS0699009.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            23269..23760
                     /gene="rocS"
                     /locus_tag="ACL719_001020"
     CDS             23269..23760
                     /gene="rocS"
                     /locus_tag="ACL719_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGS0699010.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            23858..24448
                     /locus_tag="ACL719_001021"
     CDS             23858..24448
                     /locus_tag="ACL719_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGS0699011.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            24457..26220
                     /locus_tag="ACL719_001022"
     CDS             24457..26220
                     /locus_tag="ACL719_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGS0699012.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMDRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         8020 a         4795 c         5788 g         7781 t
ORIGIN      
        1 ctcaaatctg atgggaaaat agctgaaaaa gaatgggtct acgattctca tagtcaagcc
       61 tggtactact tcaaatctgg tggctacatg gcgaaaaatg agacagtaga tggttatcag
      121 cttggaagcg atggtaaatg gcttggagga aaaactacaa atgaaaatgc tgcttactat
      181 caagtagtgc ctgttacagc caatgtttat gattcagatg gtgaaaagct ttcctatata
      241 tcgcaaggta gtgtcgtatg gctagataag gatagaaaaa gtgatgacaa gcgcttggct
      301 attactattt ctggtttgtc aggctatatg aaaacagaag atttacaagc gctagatgct
      361 agtaaggact ttatccctta ttatgagagt gatggccacc gtttttatca ctatgtggct
      421 cagaatgcta gtatcccagt agcttctcat ctttctgata tggaagtagg caagaaatat
      481 tattcggcag atggcctgca ttttgatggt tttaagcttg agaatccctt ccttttcaaa
      541 gatttaacag aggctacaaa ctacagtgct gaagaattgg ataaggtatt tagtttgcta
      601 aacattaaca atagcctttt ggagaacaag ggcgctactt ttaaggaagc cgaagaacat
      661 taccatatca atgctcttta tctccttgcc catagtgccc tagaaagtaa ctggggaaga
      721 agtaaaattg ccaaagataa gaataatttc tttggcatta cagcctatga tacgacccct
      781 tacctttctg ctaagacatt tgatgatgtg gataagggaa ttttaggtgc aaccaagtgg
      841 attaaggaaa attatatcga taggggaaga actttccttg gaaacaaggc ttctggtatg
      901 aatgtggaat atgcttcaga cccttattgg ggcgaaaaaa ttgctagtgt gatgatgaaa
      961 atcaatgaga agctaggtgg caaagattag tactataagt gaatatgatt tgagtgaata
     1021 gtaagttaaa aatcctgatt tcaagtaaaa tcaggatttt ttcatggatg taattttttt
     1081 ggagtctggt gtgacgcgga gggtcttttg tcctgtgtaa gtgacaaagc cgggttttcc
     1141 accagttggt ttattgagtt ttttgacttc aatcatatct acctgcacca gattcgacag
     1201 gcgcccttga gagaagtagg cagctaactc tgctgcgtct gtcttgactg catcagatgg
     1261 gtcaagattt cctgagatga caacatggct tccaggaatg tccttagcat ggaaccaaag
     1321 ttcctccttg cgggccattt taaaggtcaa ttcctcattt tgaagattgt ttcgtccgac
     1381 atagatgatg gttttgccat cgcttgctag atattgttct agttttttgc gtttctggat
     1441 tttctcccgt tgtcttctgc ggataaaacc tgtttgaatc aattcttcac ggatttcagc
     1501 gatttcttcc agtccagctt ggttgaggac ggtttctaca ctttccagat agagaatagt
     1561 ggctttggtt tcttcaatca aatcagtcaa gtatttgaca gcttctttga gtttctgata
     1621 ccgtttaaaa tagcgttggg cattctggtt gggagtcaga gccttatcaa gcgcaatcat
     1681 gataggttgg ttggtatagt agttgtctag gataacctgg tcttggtcgt taggcacttg
     1741 gtggaggaag gttgtcagca attctccttt ttgacgaaat tcttcagcgt tgtctgtcgc
     1801 cagtaattct ttttcctgtt ttttgagttt gtgtcggttt ttctgaagtt cattttcaac
     1861 acgacgaatc agttcactgg cctgctgttt gacgcggtcg cgctcagcct tatccttata
     1921 gtaggtgtcc aacaaatcag aaagatttgc aaaaggctct cccacctgat ttgcaaaagg
     1981 aactggactg aaggaagtct cagtcaagca tggcttggtt tcctgattga aaaaatttcg
     2041 gaaagcggaa agtttttcac taaccagtat cctttccaat tcatttgccg tatcgcgtcc
     2101 cagaccttga aagaggcttt gaagattttt tgctgttagt tcttgggttt gcaggatttc
     2161 aaagagcttt tcatccttga tagtaaaagg attgagagat tctgtacttg gcggagcgat
     2221 ataggtcgat cctggaagta aggtgcggta gctattttgt gaaaagccga cgtgtttgat
     2281 aacttcgagg attttatgac tgcttttatc gaccagtaga atattactgt gtttccccat
     2341 aatttcgata atcaaggtag cctggatatg gtctccaatc tcgtttttat tggaaactgt
     2401 catttccaca atacggtcat tttccacttg ctcaatcgac tcaatcaggg ccccctgcaa
     2461 atactttctc aaaaccatga taaaggtaga aggttgggct ggattttcaa aagtcgtttg
     2521 ggtcagctga atgcgtccaa aaactggatg ggcagaaagg agcaggcgat ggctttggcg
     2581 attgctgcgg atttgcaaga ccaactcttg ttcaaaaggc tgattgattt tctggatgcg
     2641 accattcact aattcgcttc gcaattcctc aactatgtgg tgtaaaaaaa atccgtcaaa
     2701 tgacatcgtt ctctccttgt gattgtattc catagtatta tatcaaaaag gtagaataaa
     2761 atcatggaaa tgtggtataa taaagccaag taaagagaaa cgagaagcac atgtatattg
     2821 aaatggtaga tgaaactggt caagtttcaa aagaaatgtt gcaacaaacc caagaaattt
     2881 tggaatttgc agccaaaaaa ttaggaaaag aagacaagga gatggcagtc acttttgtga
     2941 ccaatgagcg tagtcatgaa cttaatctgg agtaccgtga caccgaccgt ccgacagatg
     3001 tcatcagcct tgagtataaa ccagaattgg aaattgcctt tgacgaagag gatttgcttg
     3061 aaaatccaga attggcagag atgatgtctg agtttgatgc ctatattggg gaattgttca
     3121 tctctatcga taaggctcat gagcaggccg aagaatatgg tcacagcttt gagcgtgaga
     3181 tgggcttctt ggcagtacac ggctttttac atattaacgg ctatgatcac tatactccgg
     3241 aagaagaagc ggagatgttc ggtttacaag aagaaatttt gacagcctat ggactcacaa
     3301 gacaataaac gaaaatggaa aaatcgtgac ctgatatcca gtttagaatt tgccatcaca
     3361 gggattttta ctgctatcaa ggaagaacgc aatatgcgaa aacatgcagt gacggctcta
     3421 gtggtcatcc ttgcaggttt tggttttcag gtgtcacgaa tcgaatggct ctttctccta
     3481 ttgagcattt tcttggtggt agcctttgaa attatcaatt ctgctattga aaatgtggtg
     3541 gatttggcca gtcactatca cttttccatg ctggctaaaa atgccaagga tatggcggct
     3601 ggcgcggtat tagtggtttc tctttttgca gccttaacag gcgcattgat ttttctccca
     3661 cgaatctggg atttattatt ttaaacagta agaggaaatt atgactttta aatcaggctt
     3721 tgtagccatt ttaggacgtc ccaatgttgg gaagtcaacc tttttaaatc acgttatggg
     3781 gcaaaagatt gccatcatga gtgacaaggc gcagacaacg cgcaataaaa tcatgggaat
     3841 ttacacgact gataaggagc aaattgtctt tatcgacaca ccagggattc acaaacctaa
     3901 aacagctctc ggagatttca tggttgagtc tgcctacagt acccttcgcg aagtggacac
     3961 tgttcttttc atggtgcctg ctgatgaagc gcgtggtaag ggggacgata tgattatcga
     4021 gcgtctcaag gctgccaagg ttcctgtgat tttggtggtg aataaaatcg ataaggtcca
     4081 tccagaccag ctcttgtctc agattgatga cttccgtaat caaatggact ttaaggaaat
     4141 tgttccaatc tcagcccttc agggaaataa cgtgtctcgt ctagtggata ttttgagtga
     4201 aaatctggat gaaggtttcc aatatttccc gtctgatcaa atcacagacc atccagaacg
     4261 tttcttggtt tcagaaatgg ttcgcgagaa agtcttgcac ttaactcgtg aagagattcc
     4321 gcattctgta gcagtagttg ttgactctat gaaacgagac gaagagacag acaaggttca
     4381 catccgtgca accatcatgg tcgagcgcga tagccaaaaa gggattatca tcggtaaagg
     4441 tggcgctatg cttaagaaaa tcggtagcat ggcccgtcgt gatatcgaac tcatgctagg
     4501 agacaaggtc ttcctagaaa cctgggtcaa ggtcaagaaa aactggcgcg ataaaaagct
     4561 agatttggct gactttggct ataatgaaag agaatactaa gtagaggtag gctcatgcct
     4621 gcttcttgtt tttacagaag gaggacttat gcctgaatta cctgaggttg aaaccgtttg
     4681 tcgtggctta gaaaaattga ttataggaaa gaagatttcg agtatagaaa ttcgctaccc
     4741 caagatgatt aagacggatt tggaagagtt tcaaagggaa ttgcctagtc agattatcga
     4801 gtcaatggga cgtcgtggaa aatatttgct tttttatctg acagacaagg tcttgatttc
     4861 ccatttgcgg atggagggca agtattttta ctatccagac caaggacctg aacgcaagca
     4921 tgcccatgtt ttctttcatt ttgaagatgg tggcacgctt gtttatgagg atgttcgcaa
     4981 gtttggaacc atggaactct tggtgcctga ccttttagac gcctacttta tttctaaaaa
     5041 attaggtcct gaaccaagcg aacaagactt tgatttacag gtctttcaat ctgcccttgc
     5101 caagtccaaa aagcctatca aatcccatct cctagaccag accttggtag ctggacttgg
     5161 gaatatctat gtggatgagg ttctctggcg agctcaggtt catccagcta gaccttccca
     5221 gactttgaca gcagaagaag cgactgccat tcatgaccag accattgctg ttttgggcca
     5281 ggctgttgaa aaaggtggct ccaccattcg gacttatacc aatgcctttg gggaagatgg
     5341 aagcatgcag gactttcatc aggtctatga taagactggt caagaatgtg tacgctgtgg
     5401 taccatcatt gagaaaattc aactaggtgg acgtggaacc cacttttgtc caaactgtca
     5461 aaggagggac tgatgggaaa aatcatcgga atcactgggg gaattgcctc aggtaagtca
     5521 actgtgacaa attttctaag acagcaaggc tttcaagcag tggatgccga cgcagtcgtc
     5581 caccaactac agaaacctgg tggtcgtctg tttgaggctc tagtacagca ctttgggcaa
     5641 gaaatcattc ttgaaaacgg agaactcaat cgccctctcc tagctagtct catcttttca
     5701 aatcctgaag agcaaaaatg gtctaatcaa attcaagggg agattatccg tgaggaactg
     5761 gctactttga gagaacagtt ggctcagaca gaagagattt tcttcatgga tattccccta
     5821 ctttttgagc aggactacag cgattggttt gctgagactt ggttggtcta tgtggaccga
     5881 gatgcccaag tagaacgctt aatgaaaagg gaccagttgt ccaaagatga agctgagtct
     5941 cgtctggcag cccagtggcc tttagaaaaa aagaaagatt tggccagcca ggttcttgat
     6001 aataatggca atcagaacca gcttcttaat caagtgcata tccttcttga gggaggtagg
     6061 caagatgaca gagattaact ggaaggataa tctgcgtatt gcctggtttg gtaattttct
     6121 gacaggagcc agtatttctt tggttgtacc ttttatgccc atcttcgtgg aaaatctagg
     6181 tgtagggagt cagcaagtcg ctttttatgc aggcttagca atttctgtct ctgctatttc
     6241 cgcggcgctc ttttctccta tttggggtat tcttgctgac aaatacggcc gaaaacccat
     6301 gatgattcgg gcaggtcttg ctatgactat cactatggga ggcttggcct ttgtcccaaa
     6361 tatctattgg ttaatctttc ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa
     6421 tgcaacggca ctgatagcca gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac
     6481 tttgtctaca ggcgtagttg caggtactct aaccggtccc tttattggtg gctttatcgc
     6541 agaattattt ggcattcgta cagttttctt actggttggt agttttctat ttttagctgc
     6601 tattttgact atttgcttta tcaaggaaga ttttcaacca gtagccaagg aaaaggctat
     6661 tccaacaaag gaattattta cctcggttaa atatccctat cttttgctca atctcttttt
     6721 aaccagtttt gtcatccaat tttcagctca atcgatcggc cctattttgg ctctttatgt
     6781 acgcgactta gggcagacag agaatcttct ttttgtctct ggtttgattg tgtccagtat
     6841 gggcttttcc agtatgatga gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa
     6901 tcatcgtctc ttggttgtcg cccagtttta ttcagtcatc atctatctcc tctgtgccaa
     6961 tgcctctagc ccccttcaac taggactcta tcgtttcctc tttggcttgg gaaccggtgc
     7021 cttgattccc ggggttaatg ccctactcag caaaatgact cccaaagccg gcatttcgag
     7081 gatctttgcc ttcaatcagg tattctttta tctgggaggt gttgttggtc ccatggcagg
     7141 ttctgcagta gcaggtcaat ttggctacca tgctgtcttt tatgcgacaa gcctttgtgt
     7201 tgcctttagt tgtctcttta acctgattca atttcgaaca ttattaaaag taaaggaaat
     7261 ctagtgcgag taaaaattaa tctcaaatgc tcctcttgtg acagtatcaa ttacctaacc
     7321 agtaaaaatt caaaaaccca tccagacaag attgaggttt taaagtattg tcccaaggaa
     7381 agaaaagtaa ccctacatct tgaatctaag tagattttat ggtaaaataa tagggatttt
     7441 aaggagtttg atatgtataa cctattatta accattttat tagtattatc tgttgtgatt
     7501 gtgattgcaa ttttcatgca accaaccaaa aaccaatcca gcaatgtatt tgatgccagt
     7561 tcaggtgatt tgtttgaacg cagtaaagct cgcggttttg aagctgtaat gcagcgtttg
     7621 acagggattt tagtcttttt ctggctagcc attgccttag cattgacggt attatcaagt
     7681 agataagaaa ataatgggca ggactaggtc tttgcctctt tttattttta aaggatgttt
     7741 gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa agaaaatatg aaagatagaa
     7801 taaaagaata tttacaagac aagggaaagg tgactgttaa tgatttggct cagactttgg
     7861 gaaaagacag ttccaaggat tttcgtgagt tgattaaaac cttgtcctta atggaaagaa
     7921 agcaccagat tcgctttgaa gaagatggta gtctgacatt agaaattaag aaaaaacatg
     7981 agattaccct caaggggatt tttcatgccc ataaaaatgg ctttggcttt gttagtctgg
     8041 aaggcgagga ggacgacctt tttgtaggga aaaatgatgt caactatgct attgatggtg
     8101 ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa taagggaaca gcagcagaag
     8161 ccaaaattat tgatatccta gaacacagtt tgacaacagt tgtcgggcaa atcgttctgg
     8221 atcaggaaaa gcctaagtat gctggctata ttcgttcaaa aaatcagaaa atcagtcaac
     8281 cgatttatgt taagaaacca gccctaaagt tagaaggaac agaagttctc aaggtcttta
     8341 tcgataaata cccaagcaag aaacatgatt tctttgtcgc gagtgttctc gatgtagtgg
     8401 gacactcaac ggatgtcgga attgatgttc ttgaggtctt ggaatcaatg gacattgtat
     8461 ccgagtttcc agaagctgtt gttaaggaag cagaaagtgt gcctgatgct ccgtctcaaa
     8521 aggatatgga aggtcgtctg gatctaagag atgaaattac ctttaccatt gacggtgcgg
     8581 atgccaagga cttggacgat gcagtgcata tcaaggctct gaaaaatggc aatctggagc
     8641 ttggggttca catcgcagat gtttcttatt atgtgaccga ggggtctgcc cttgacaagg
     8701 aagcccttaa ccgtgcgact tctgtttacg tgacagaccg agtggtgcca atgcttccag
     8761 aacgactatc aaatggcatc tgctctctca atccccaagt tgaccgcctg acccagtctg
     8821 ctattatgga gattgataaa catggtcgtg tggtcaacta taccattaca caaacagtta
     8881 tcaagactag ttttcgtatg acctatagcg atgtcaatga tatcctagct ggcgatgaag
     8941 aaaagagaaa agaatatcat aaaattgtat caagtatcga actcatggcc aagcttcatg
     9001 aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa ttttgatacc aatgaagcga
     9061 agattttagt ggataaacaa ggtaagcctg ttgatatcgt tcttcggcag cgtggtattg
     9121 ccgagcggat gattgagtct tttatgttga tggctaatga aacagttgcc gaacatttca
     9181 gcaagttgga tttgcctttt atctatcgaa ttcacgagga gcctaaggct gaaaaggttc
     9241 agaagtttat tgattatgct tcgagttttg gcttgcgcat ttatgggact gccagtgaga
     9301 ttagtcagga ggcacttcaa gacatcatgc gtgctgttga gggagaacct tatgcagatg
     9361 tattgtccat gatgcttctt cgctctatgc agcaggctcg ttattcggag cacaatcacg
     9421 gccactatgg actagctgct gactattata ctcactttac cagtccaatt cgtcgttatc
     9481 cagaccttct tgttcaccgt atgattcggg attacggccg ttctaaggaa atagcagagc
     9541 attttgaaca agtgattcca gagattgcga cccagtcttc caaccgtgaa cgtcgtgcca
     9601 tagaagctga gcgtgaagtt gaagccatga aaaaggctga gtatatggaa gaatacgtgg
     9661 gtgaagagta tgatacagtt gtatcaagta ttgtcaaatt cggtctcttt gtcgaattgc
     9721 caaacacagt tgaaggcttg attcacatca ctaatctacc tgaattttat catttcaatg
     9781 agcgtgattt gactcttcgt ggagaaaaat caggtatcac tttccgagtg ggtcagcaga
     9841 tccgtatccg tgttgaaaga gcggataaaa tgactggaga gattgatttt tcattcgtac
     9901 ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc tagtcgtagt ggcagagggc
     9961 gtggttcaaa tcgtcgttcg gataagaagg aagacaagag aaaattagga cgctcaaatg
    10021 ataagcgtaa gcattcacaa aaagacaaga agaaaaaagg aaagaaacct ttttacaagg
    10081 aagtagctaa gaaaggagcc aagcatggca aagggcgagg gaaaggtcgt cgcacaaaat
    10141 aaaaaggcac gccacgacta tacaatcgta gatacgctag aggcagggat ggtcctgact
    10201 ggaactgaaa tcaagagtgt acgagctgct cgaattaatc tcaaggatgg ctttgctcaa
    10261 gtgaaaaatg gagaagtttg gctgagcaat gttcatatcg cgccttacga agagggcaat
    10321 atctggaacc aggaaccaga acgtcgtcgt aaactcctgc tccataaaaa gcaaattcaa
    10381 aaattggaac aagagaccaa agggacagga atgaccttag ttccccttaa ggtctatata
    10441 aaagatggct acgctaagct tcttttagga cttgccaaag ggaagcatga ctatgacaaa
    10501 cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg cgcgtgtgat gaaagctgtt
    10561 aatcagcgat aaaaagagga attgaaaatg gaaaaattag ttgcctataa acgcatgcct
    10621 ttgtggaata aacaaacaat gcctgaagct gttcagcaaa agcacaatac aaaagttggg
    10681 acttggggga aaattactgt cttgaaggga gctctcaagt ttattgaatt gacagaagaa
    10741 ggggaagttc tagctgaaca cctctttgaa gcaggggcag acaatccaat ggcccaacct
    10801 caagcctggc accgagtgga agctgccaca gatgatgtgg aatggtactt ggaattttat
    10861 tgtaaacctg aggattattt tgctaaaaaa tacaatacca atcctgttca ttcagaggtc
    10921 ctagaggcca tgcagacagt gaaacaaggg aaagctttgg atttaggttg tggtcagggg
    10981 cgtaattctc tttttctagc ccagcaagat tttgatgtga cagctgtaga tcaaaatgga
    11041 ctagctcttg aaatcttgca aagcattgtg gagcaggaag atttggacat gcctgttggc
    11101 ctttacgata tcaattcagc tagcattgaa caagaatatg attttatcgt ttcaacagtt
    11161 gttctcatgt ttctacaagc ggaccgcatt ccagctatta ttcaaaatat gcaggagaaa
    11221 accagtgttg gtggttacaa ccttatcgtt tgtgccatgg acacggagga ttatccttgc
    11281 tcggttaact tcccattcac ctttaaagaa ggagaactgg cagactatta caaggattgg
    11341 gaattggtta agtacaatga aaatccaggc catttgcatc gtcgcgatga gaatggcaat
    11401 cgtattcaac tacgctttgc gaccttacta gctaagaaaa tcaagtaaac acacatgaag
    11461 attaggaatt ttcctgatct tttttctttt ttacgaatga tatagaaaag gagggaattc
    11521 atgtttgttg cgagagatgc taggggagaa ttggtaaatg tgttagagga taaacttgag
    11581 aagcaagcat acacctgccc agcttgtgga ggccagctcc atttgcgtca aggaccaagt
    11641 gtacggacgc attttgccca taaatcctta aaagactgtg attttttctt tgaaaatgaa
    11701 agtccagaac acctggccaa taaggaatcc ctctatcact ggttgaaaaa agagacaaag
    11761 gttcaattag agtacccgct ttcagaactt aaacagattg cggatgtatt tgtaaatggc
    11821 aatctagctc tagaagttca gtgtagtccc ttgcctcaga aagtccttaa agagcgaagt
    11881 gagggctatc gtagtcaggg ttaccaagta ctgtggttgc tgggtcaaaa actgtggctc
    11941 aaggagcgtt tgactcgtct acagcaaggt tttctttatt tcagtcaaaa catgggcttt
    12001 tatgtttggg aattagacaa ggaaaaacaa gttttaagac tcaaatacct gatttaccag
    12061 gatctccgcg gtaaactcca ttatcaaatc aaggaatttt cctatggtca aggtagttta
    12121 ttggaaatat tgcgtcttcc ctataagaga caaaaaatat ctcattttac agtttctgag
    12181 gacaaggaca tctgtcgcta tatccggcaa caactttatt atcaaaatct cttttggatg
    12241 aaagaacaag cagaagccta tcaaaaggga gaaaatatcc tgacttatgg actgaaagaa
    12301 tggtatccac aaattcgacc aatagtgggc aaatttttcc agattgaaca agacttgact
    12361 agctattatc agcactttta tacctattac caaaaaaatc ctcaaaatga ttggcaaaag
    12421 ctttatccac cagcctttta tcagcaatat ttcttgaaaa atatggtaga atagaaagga
    12481 tggaggaatc taatggtatt acaaagaaat gaaataaatg aaaaagatac atgggatcta
    12541 tcaacgatct acccaactga ccaggcttgg gaagaagcct taaaagattt aacagaacaa
    12601 ttggagacag tagcccagta tgaaggccat ctcttggata gtgcggataa cctactagaa
    12661 atcactgaat tttctcttga aatggaacgc cagatggaga agctttacgt ttatgctcat
    12721 atgaagaatg accaggatac acgtgaagct aagtatcaag agtactatgc caaggccatg
    12781 acactctaca gccagttaga ccaagccttt tcattctatg agcctgaatt tatggagatt
    12841 agcgaaaagc agtatgctga ctttttagaa gctcaaccaa agctgcaggt ttatcaacac
    12901 tattttgaca agctcttgca aggcaaggat cacgttcttt cacaacgtga agaagaatta
    12961 ttggctggag ctggagaaat ctttggttca gcaagtgaaa ccttcgctat cttggacaat
    13021 gcggatattg tgttccctta tgtcctagac gatgatggta aagaagttca gctatctcat
    13081 gggacttaca cacgtttgat ggagtctaaa aaacgtgagg ttcgccgtgg tgcctatcaa
    13141 gctctttatg cgacttacga acaattccaa cacacctatg ccaaaacttt gcaaaccaat
    13201 gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca agagtgctcg tcatgcagcc
    13261 ctcgcagcga attttgttcc agaaagtgtt tatgacaatt tggtagcagc agttcgcaag
    13321 catttgccac tcttacatcg ctatcttgag cttcgttcaa aaatcttggg gatttcagat
    13381 ctcaagatgt acgatgtcta cacaccgctt tcatctgttg aatacagttt tacctaccaa
    13441 gaagccttga aaaaagcaga agatgctttg gtagtcttgg gtgaggatta cttgagccgt
    13501 gttaaacgtg ccttcagcga gcgttggatt gatgtttacg aaaatcaagg caagcgttca
    13561 ggtgcctact ctggtggttc ttacgatacc aatgccttta tgcttctcaa ctggcaggac
    13621 aatctggaca atctctttac tcttgttcat gaaacaggtc acagtatgca ttcaagctat
    13681 actcgtgaaa ctcagcctta tgtttacgga gattactcta tctttttggc tgagattgcc
    13741 tcaactacca atgaaaatat cttgacggag aaattattgg aagaagtgga agacgacgca
    13801 acacgctttg ctattctcaa taacttccta gatggtttcc gtggaacagt tttccgccaa
    13861 actcaatttg ctgagtttga acacgccatt caccaagcag atcaaaatgg ggaggtcttg
    13921 acaagcgatt tcctaaataa actctacgca gacttgaacc aagagtatta tggtttgagt
    13981 aaggaagaca atcctgaaat ccaatacgag tgggctcgca ttccacactt ctactataac
    14041 tactatgtat atcaatattc aactggcttt gcggccgcct cagccttggc tgaaaaaatt
    14101 gtccatggta gtcaagaaga ccgtgaccgc tatatcgact acctcaaggc aggtaagtcg
    14161 gactatccac ttaatgtcat gagaaaagct ggtgttgata tggagaagga agactacctc
    14221 aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt ttgaagccct tgttgaaaaa
    14281 ttaggattgg cataaaatgg ttgaatcgta tagtaagaat gctaaccata acatgcgtcg
    14341 tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag cgtcaaaagc aggtcacagg
    14401 tttcttgaaa gaattggaag actttgcccg caaggaaaat attcctatta ttccccatga
    14461 aacggttgct tatttccgtt ttcttatgga aaccatgcag cctaaaaata ttctggaaat
    14521 tgggacggct atcggttttt cagctctctt gatggctgaa catgcgccaa atgctaagat
    14581 tacaactatt gatcgtaatc cagaaatgat tggttttgcc aaggaaaatt ttgcccagtt
    14641 tgacagtcgc aagcaaatca ctctcctaga gggagatgcg gtggatgtct tatctacact
    14701 gacagagtct tatgatttcg tctttatgga ttctgccaag tctaaataca tcgtctttct
    14761 gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt gtcttggatg atatttttca
    14821 aggcggtgat gttgccaagg acattatgga agtccgtcga ggtcagcgaa ccatctatcg
    14881 aggccttcaa agactatttg acgcaacctt agacaattca gaactcaccg caacattagt
    14941 gcctttagga gatggtattc tcatgcttcg taaaaatgta gcagatgttc aattgcctga
    15001 cagcgaatga ttttcagaaa aatttaagaa aaaatagtaa aatagataga gtaacactta
    15061 tctcaaagga gtagacatga agaaaaaatt attggcaggt gccatcacac tattatcagt
    15121 agcaacttta gcagcttgtt cgaaagggtc agaaggagca gaccttatca gcatgaaagg
    15181 ggatgtcatc acagaacatc aattttatga gcaagtgaaa aacaaccctt cagcccaaca
    15241 agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa caatatggct cagagcttga
    15301 tgataaagag gttgatgata ctattgccga agaaaaaaaa caatatggcg aaaactacca
    15361 acgtgtcttg tcacaagcag gtatgactct tgaaacacgt aaagctcaaa ttcgtacaag
    15421 taaattagtt gagttggcag ttaagaaggt agcagaagct gaattgacag atgaagccta
    15481 taagaaagcc tttgatgagt acactccaga tgtaacggct caaatcatcc gtcttaataa
    15541 tgaagataag gccaaagaag ttctcgaaaa agccaaggca gaaggtgctg attttgctca
    15601 attagccaaa gataattcaa ctgatgaaaa aacaaaagaa aatggtggag aaattacctt
    15661 tgattctgct tcaacagaag tacctgagca agtcaaaaaa gccgctttcg ctttagatgt
    15721 ggatggtgtt tctgatgtga ttacagcaac tggcacacaa gcctacagta gccaatatta
    15781 cattgtaaaa ctcactaaga aaacagaaaa atcatctaat attgatgact acaaagaaaa
    15841 attaaaaact gttatcttga ctcaaaaaca aaatgattca acatttgttc aaagcattat
    15901 cggaaaagaa ttgcaagcag ccaatatcaa ggttaaggac caagccttcc aaaatatctt
    15961 tacccaatat atcggtggtg gagattcaag ctcaagcagt agtacatcaa acgaatagtc
    16021 caaatcaatg agtcagggaa aaaactcgac ttcaggaaaa aatgaagcaa acattcccac
    16081 aataaaacgc atagtacaag gtttgtactg ccccccaaaa aagttagaca attaatttat
    16141 ccgaaggatt tagttctgta ttgcacagag ctaagtcctt ttagttttat cttaattctc
    16201 ttattgttgt aataatcaat atagtctata atggcttgtt ccaattgatt aagtgattta
    16261 aatgtttgct catagccata aaacatttcg gattttaaaa tgccaaagaa agattccatc
    16321 ctaccgttgt cttggctgtt tcctttgcgt gacatagatg cttgaattcc cttactctct
    16381 aggaagcgat gataagaatc gtgttggtat tgccagcctt ggtcactatg gagaatcgta
    16441 ttctcgtagt gcttctcttt gaatgcctgt tccaacatta acgatcaatc aatttaatca
    16501 tgtacctaat attagaattg tttatctcaa atttatttga aagtttctct aagctatatc
    16561 cttgttttct aagttcatag atctgaactt tatcatcata agttaatttc ataataaaaa
    16621 caccccaaaa gttagatttt ttctgtctaa cttttggggg gcagttcatt taacacctga
    16681 taatatgcgt tttttgattt taaagacttt ttgaccagtc tcattttttt aacttgatac
    16741 tcagtgcaaa gcaaagatta aactaggaag ctagctgtag gctgctcaaa gaacagcttt
    16801 gaggttgtag ataaaacttg tgaggtcacc aacatatata atgtgaagct gacgtggttt
    16861 gaatagattt tagaagagta tgagtctgga agttttaatg gataatgcaa gattccatag
    16921 aatgggtaag ctagagttct tatgtgaaga gtttgggcat aaacttttac cttttcctcc
    16981 ctactcatct tagtatagaa aagtgaatct gaaatagtac ataactgctt ctaaaacatt
    17041 cttataaatt gatttaaatt ctcaaatcat attattcagt tcttatttca ttttgttcta
    17101 caatcctgtt gagaagacac gtgttcatat caaaaaggta ttggcaagtt gcaatacctt
    17161 tttacgaggc tctgttgtct tatttttgtt tcaactgact atatctccta tggttctagt
    17221 tcagaaggct aggctataat tatgattgat aagaagtatc attccaagta ttgggagtga
    17281 atgtttcaaa atcatgggtt tctataatgg tcaggctggc atttgctaga ccgccatctt
    17341 tacgaagaag tggttcttta tagcctagga gagtacgaag actggcagta agattggcgc
    17401 cgtgtccgac aattagaata cgttcagctg gactatcttt taatgatttg ataaattgga
    17461 tggtccgttg agttgtacta tagagggatt cggctccgaa cattcgagtg tcaaattgag
    17521 caagatttga acgaaaagcc tggatttgtt gcgggtaaat agcttccaag gttgcaattt
    17581 tcaaaccttc taacttccca agttgccatt cacggagatt aggaacgatt tctaaagaac
    17641 agggggtata gagttgactt tggataatct cagcagattt gaccgctcga ggtaaatcac
    17701 ttgaataaat ctgatcaaaa ggaatttcct tgagatactg accaagtcgt tttagggttt
    17761 caatggattc aggaagaaga ggagaatcac cactagcacc ttgaaaacga ccttcttggt
    17821 tccagagggt acgaccgtgg cggacaaagt agagtttcat tacttgatgt cctccaaaat
    17881 atctacaaag tctgccttta caaagctagc caagtcttgt ggcgcgacga taatgctgtg
    17941 tccgacttcg cctgcagaga caatcatttg atccaaatct agagcaattt tatcgataaa
    18001 aatgggataa ttgtgtttct gacgaattcc gacaggatta ttggctccat gaatgtaacc
    18061 agttgttttt tctaagtcct tttgtggaat catgctcact tttttattgc cagaaatttt
    18121 agctagtttc ttttcagaca agtgctgagt gatagggaca attccgataa tcggtccggt
    18181 cttgtctccc aaaagcgcca aggttttgaa aatctgatct cgttcataac cttgaggaag
    18241 ctctccttct agggcattga tttgaatccc ctgatgaggg atagctgctt tagataggat
    18301 ttgttccacc aatgtttttt tgattttaac tttttttgcc attatttata tttatcctcc
    18361 aattgactca tccaaatacc aagccagatt cccagcgcaa agaagaaggc gatgatgaca
    18421 taaccgacaa gtgaaagtcc tgtgtattgg atactttcag cgtttcctgc atttggaatt
    18481 aagatcaaaa gggtacttga taggacgata ccgatgatga aatgatagac gtgtgagtgg
    18541 tagttgttta aggcataatc catcaatttt gaaaaaacga tgagagttgc acctgcacca
    18601 attccaatcg gaaagaaggt tcccaagaga tcaaaagttt taaaaccagt caacatagga
    18661 gcatagagtc ccaaaatcaa aagtaagttc gatgggctga ggccaggaac caagacgcca
    18721 agggccaata gtgcgcctgc taggacgaag ttaagaaagc tggcgcttaa ggttccaacg
    18781 acaaaattta aggcatagag tcctaatcca gaaatgataa aggttgtcca taaccaagct
    18841 aaatcaatct tgtctcggtc agattctcga gttgattctt tgaggaggct aggaactgta
    18901 ccgataatag ctcccgcaaa gctccataat acaaaaacct gataattttc aagcaggtat
    18961 tcaatcgggt aggaaaataa gccgattccc agaagcatac cgatggcaac tggaataaag
    19021 tacaaaacat tttctttaaa gtctttaaag ggatgggcca gaaagccaat cattcgttca
    19081 tagattccta agattgctgc tagaacccct ccggaaattc ccggtaggat aaatccaaga
    19141 gcaattacaa tccctttaat aacgcgtgct aaccatgaga gcatatattt cctccaacta
    19201 tttctatttc aaaaaatcct tactatattg tatcacaatc agacacaaaa agaaaaagca
    19261 aatgataaac aaatgctttt aaggttttaa aaaaagtttt cgaaaggttt cttctttatt
    19321 ttttaaggga gagataacgt tgatatctaa atcgtggtca aagccagcaa tttttccttt
    19381 agatgtgtat tggtgaatat cataatctaa atcagtttta ggactgctct ccaaaaatcc
    19441 tgagtctgag ccgtaggacg gaatccaaac agaggtaaac ttgcctgtat caatactgtg
    19501 ttcttccatg aagtagacac caacgtagat gccgatgttt ttagcaccta gtgatgctag
    19561 ttttgctcga aagttttcga caccttcgtt catattagac atggttttgt cttccacgtc
    19621 aagccaatag taactagggc tgtaaggaga ggcagcattg tagaaaactt cggcagcctt
    19681 ttccatttct tggacacttt ttccagctac ataagcgtag acagcaactg ggacattccg
    19741 cttttgaagt tcagtgatat gactcttata ggccttgtct attccattga taaatgaagc
    19801 atcattttct tttgttgttt gagcaccact gtgaacacga acaatagcac ctgaaatatt
    19861 ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc cagccagaga ggtcaataat
    19921 cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt gctatattgg attttgtttt
    19981 aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa atgaacatca taatcccaaa
    20041 aaaactaaat aaaataagtg gatgaatttg ttttctcata tcttataatt ctaccctaaa
    20101 aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt tagagcattt tttcattcaa
    20161 gagtgcggaa tgatttgaaa tatggtataa taaaagggaa tttctacaga aaagagaaga
    20221 ttatgtcaaa ttttgccatt attttagcag cgggtaaagg gactcgcatg aaatctgatt
    20281 tgccaaaagt tttgcacaag gttgcgggta tttctatgtt ggaacatgtt ttccgtagtg
    20341 tgggagctat ccaacctgaa aagacagtaa cagttgtagg acacaaggca gaattggttg
    20401 aggaggtctt ggctgaacag acagaatttg tgactcaatc tgaacagttg ggaactggtc
    20461 atgcagttat gatgacagag cctatcttag aaggtttgtc aggacacacc ttggtcattg
    20521 caggagatac tcctttaatc actggtgaaa gcttgaaaaa cttgattgat ttccatatca
    20581 atcataaaaa tgtggccact atcttgactg ctgaaacgga taatcctttt ggctatggac
    20641 gaattgttcg taatgacaat gctgaggttc ttcgtattgt tgagcagaag gatgctacag
    20701 attttgaaaa gcaaatcaag gaaatcaaca ctggaacata cgtctttgac aacgagcgtt
    20761 tgtttgaggc tttgaaaaat atcaatacca ataacgctca aggcgaatac tatattacag
    20821 acgtcattgg tattttccgt gaaactggtg aaaaagttgg cgcttatact ttgaaagatt
    20881 ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc gacagctgag tcagttatgc
    20941 gtcgtcgcat caatcataaa cacatggtca acggtgttag ctttgtcaat ccagaagcaa
    21001 cttatatcga tattgatgtt gagattgctc cggaagttca aatcgaagcc aatgttatct
    21061 tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac aaacggtact tatgtagtgg
    21121 acagcactat cggagcagga gcggtcatta ccaattctat gattgaggaa agtagtgttg
    21181 cagacggtgt gacagtcggt ccttatgctc acattcgtcc aaattcaagt ctgggtgccc
    21241 aagttcatat tggtaacttt gttgaggtga aaggatcttc aatcggtgag aataccaagg
    21301 ctggtcattt gacttatatc ggaaactgtg aagtgggaag caacgttaat ttcggtgctg
    21361 gaactattac agtcaactat gacggcaaaa acaaatacaa gacagtcatt ggaaacaatg
    21421 tctttgttgg ttcaaattca accattattg caccagtaga acttggtgac aattccctcg
    21481 ttggtgctgg ttcaactatt actaaagacg tgccagcaga tgctattgct attggtcgcg
    21541 gtcgtcagat caataaagac gaatatgcaa cacgtcttcc tcatcatcct aagaaccagt
    21601 aggagcctat catggagttt gaagaaaaaa cgcttagcag aaaagaaatc tatcaaggac
    21661 caatatttaa actggtccaa gatcaggttg aattaccaga aggcaaggga actgcccaac
    21721 gggatttgat ttttcacaat ggggctgtct gtgttttagc agtaacggat gaacaaaaac
    21781 ttatcttggt caagcagtac cgcaaagcta tcgaggcagt ctcttacgaa attccagccg
    21841 gaaaattgga ggtaggagaa aacacagccc ctgtcgcagc tgcccttcgt gaattagagg
    21901 aagaaacagc ctatacaggg aaattagaac tcttgtacga tttttattca gctattggct
    21961 tttgtaatga gaagttaaaa ctatatttag caagcgattt gacaaaagtg gaaaatccgc
    22021 gtccgcagga tgaggatgaa accttggaag tccttgaagt gagcttagaa gaagcgaaag
    22081 aattaatcca atcaggtcat atctgtgatg ccaagacaat tatggctgtt cagtattggg
    22141 agttgcagaa aaaatagagg aggtcagtat gggtaaatct ttattaacgg atgaaatgat
    22201 tgaaagagct aatagaggcg aaaaaatttc aggtcctcct ttgctagatg ataatgagga
    22261 aactaagatt ttaccaacct cttcttcccg ttttggttat gccaatccta aggatcatgg
    22321 ttttagccag gaaaccttga agattcaggt cgaaccatct attcataaaa gccgtcgtat
    22381 tgaaaatacc aagagaaatg tcttcaattc taagttgaat aaaatcttat tcgcggtcat
    22441 ctttctcttg attttgcttg ttttagcaat gaaacttttg taatagaaaa ggaattgaaa
    22501 tgaaaatagg aattattgct gctatgccag aagaactggc ttatctggtc cagcatttag
    22561 acaatgccca ggagcaagtt gttttgggga atacctatca tacaggaacc attgcttctc
    22621 atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat gtctgctatg agtgtggcga
    22681 ttttggctga tcatttccag gtggatgccc ttattaatac gggttcagct ggggcagtag
    22741 cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa attagcctat catgacgtgg
    22801 atgtcacagc ttttggctat gcttatggac aaatggcgca acaaccgctt tatttcgaat
    22861 cagacaaaac ctttgttgct caaatccaag agagtttatc tcaattggac caaaactggc
    22921 atcttggttt gattgctaca ggagatagtt ttgttgcagg aaatgacaag atagaagcga
    22981 ttaagtccca tttcccagaa gttttagccg tggagatgga gggggcagct attgctcaag
    23041 cagcgcatgc cctcaatctc ccagtcttag tcatcagagc tatgagtgac aatgccaacc
    23101 atgaagcaaa catctttttt gatgagttta ttatcgaagc tggacgtcgc tctgcccaag
    23161 tcttgttgac ctttttgaag gctttagatt aagtggaaat ttgacagttt ttctagctta
    23221 tgataagatt taagtaaaga aaagctagaa aacgtttcag aggatattat gagtattgaa
    23281 atgaccgtca gtgagattgc agaggtctta ggattatctc gccaagcaat caataaccgt
    23341 gtcaaagaat taccagaaga agacacagat aaaaatgaca aaggggtaac agttgttacc
    23401 agaagtggct tgattaagct agaagaaatc tataaaaaaa cgatttttga agatgaacct
    23461 gtcagtgaag atgtcaaaca acgtgaactg atggagattc tagtggatga gaagaatgca
    23521 gaaattcttc gtctttatga acaattaaaa gccaaggatc gtcagttatc agaaaaagac
    23581 gagcagatgc gtatcaaaga ccgtcagatt gctgagaaag acaaacaatt agatcagcaa
    23641 caacaattga cccttcaagc catgaaggat caagaaaatc ttaagctaga gctggaccaa
    23701 gcaaaagaag aagtccaatc cactaaaaaa ggcttttttg ctcgtttatt tggaggataa
    23761 tcaaggagct gtttaggtta aatgctaacc tgatggcttc ttttgtttgt gaatagtata
    23821 gttgattaga acagagacag tagaggagag gtacagaatg gaacgattag aagattacat
    23881 tgcttttgat ttagaattta atcagcatga aggacaaaca cacttgattc aagtatcggc
    23941 agtgcgtttt agagatggtc gggaaatcga tgcctatgat tcctatgttc ataccagcgt
    24001 ccctctaaaa agctttatca atggtttgac cgggattaca gctgagacct taaaagatgc
    24061 tcctccagtt gagaaagtct tagaagaatt ccaagagttt gtgggttctt tgctgatgat
    24121 aggctacaat gctgctaaaa gtgatttacc tattttagca gagcatggtt tagactatag
    24181 ccaacagtac aaggtggatt tgtatgatga agcttttgaa cgtcggagtt cagatttaca
    24241 tggtattgcc aatctcaaat tgcaaactgt tgcctcattt cttggatttc aaggtcagtc
    24301 tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt tacgaatcct tcttagagtc
    24361 ggatcaagct agggaattgt tagagacaga aagtagctta tcaaacaatc cttttggtgg
    24421 cttggacttg tctcaattat ttgactagga gtgcctatga aacctgaaaa acctaaaaaa
    24481 ttgggtctta aacagattta cctcaatctt gataaaatcc tctttctatt tttcttgatt
    24541 tttatgatag tggagtattt gtgggtacca ttcaattcat ggttggctag cttcttatta
    24601 gatcagacag gttatctttt tatttcctat aacaatgtct tagccatcct tactcattct
    24661 ccattgatta gtctagcttt tttagccttg attgtaatta actttctggt tgcttatttt
    24721 caaataggac tcctctttat tggtgctcgt catctcctct ataatgaaaa gcgaaggcta
    24781 attgagtata tccgcaaggt tttccatgaa agttttgaat ttatgaaaaa gctaactggc
    24841 gctaaagcct tgtttatctt tttctatata gctatgctgt ttccttttat acggaaaatg
    24901 ctgaagattt attatcttaa taaaatcgtc attccagagt ttatccaagc ttatttagaa
    24961 gataaatatt ggatgtggtg gctgagcatc ctacttctat ctcttatctt tctctatgtc
    25021 tctgtcagat tgatgtttgc ccttccgaag attgtatatg atcagctgac tgttcgagaa
    25081 gcagtgatgt ttagcttgga gaaaaccaag aagagagtta ctttttatgc ttggaattta
    25141 ttttacatct tgctcaaagc acatttatta ttttatcttc ctttgattcc tttattattg
    25201 gctcaaactt tggtagatga tatcactcag agagagtctc tgattcttgg tattttgaat
    25261 tttgttgtga ttaaaaatct ctattatatg gctctgactt attttttggt taaatttgtt
    25321 tcatttttga caggaaagga gctggatatg cttcctagga gagaaaaaga tcacatcgtg
    25381 cgatggggtg tcatgacttg tgccagtctt ttctttgcct tagaaggtta tatttatctg
    25441 gaggctccca tggttcatct acctcaactt atttctcacc gaggggtttc caatgcaaat
    25501 gggattcaaa atacagtaga gtccttggaa actacagcac aactcaaacc agacttggtg
    25561 gagacggacg tgcaggaaac aaaagatggg cagtttgtca tgatgcatga tgctaacttg
    25621 aaaaatctag caggtatcaa taaaagtcct caagacttaa acttggagga gcttaaaggg
    25681 attgatattt ttgaaaatgg ctaccagact aaaatttcaa gctttgaaga ttatctcagt
    25741 cgagccaacg aacttggtca aaaattacta attgaaatta aaaccagtaa aaaagatagt
    25801 ccagacatga tggaccgctt tttagcccgt tatgctgcaa agctcaagat ttatggacat
    25861 caaatccagt ctttagacta ccatgttgtc gaaaaagtaa gacagtatga tgccgaactg
    25921 ccagtttatt tcatcatgcc ctacaattct gtctttccta aaacaagagc gacaggatat
    25981 actatggagt actcaacctt ggatgaatat tttgtaagca aactatggac aacggatcag
    26041 aaactttatg tctggactgt caatgattca gaagctatca gcaaatctct tcacttagga
    26101 gtagatggag tgattacaga tgatttggaa aaagttcagc gggaaataga agtagcccaa
    26161 gaagatccag aatatacgga tttgctcttg aaaaaagcat tggaattctt tgaattttag
    26221 taataaaaaa gaggaacggt tgttcctccc atgataggta agtattcttg tgaatgcttg
    26281 cttttttgtg gttgcaatcc gtttacgaga attgtttgta ttgttcttgt ttcaatttac
    26341 tttatagtgc tttgaagttg gaatgctaca ctataacttc taaa
//
LOCUS       DBERCA010000020        25225 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.020, whole genome shotgun sequence.
ACCESSION   DBERCA010000020 DBERCA010000000
VERSION     DBERCA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.020"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..134)
                     /locus_tag="ACL719_001023"
     CDS             complement(<1..134)
                     /locus_tag="ACL719_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS0699013.1"
                     /translation="MSQSIVPVEIPQYCRFDSKKRNGILFNVRIANLKFTFFRLDFLR
                     "
     gene            complement(359..1219)
                     /locus_tag="ACL719_001024"
     CDS             complement(359..1219)
                     /locus_tag="ACL719_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGS0699014.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1235..2512)
                     /gene="spxR"
                     /locus_tag="ACL719_001025"
     CDS             complement(1235..2512)
                     /gene="spxR"
                     /locus_tag="ACL719_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGS0699015.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2505..3056)
                     /locus_tag="ACL719_001026"
     CDS             complement(2505..3056)
                     /locus_tag="ACL719_001026"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699016.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3072..4331)
                     /locus_tag="ACL719_001027"
     CDS             complement(3072..4331)
                     /locus_tag="ACL719_001027"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS0699017.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGVSIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGTSVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            complement(5486..5644)
                     /locus_tag="ACL719_001028"
     CDS             complement(5486..5644)
                     /locus_tag="ACL719_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699018.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(5654..6964)
                     /gene="obgE"
                     /locus_tag="ACL719_001029"
     CDS             complement(5654..6964)
                     /gene="obgE"
                     /locus_tag="ACL719_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGS0699019.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(7028..7162)
                     /locus_tag="ACL719_001030"
     CDS             complement(7028..7162)
                     /locus_tag="ACL719_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGS0699020.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(7221..7397)
                     /locus_tag="ACL719_001031"
     CDS             complement(7221..7397)
                     /locus_tag="ACL719_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS0699021.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(7428..8753)
                     /locus_tag="ACL719_001032"
     CDS             complement(7428..8753)
                     /locus_tag="ACL719_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0699022.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRSSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(8769..9812)
                     /gene="cpoA"
                     /locus_tag="ACL719_001033"
     CDS             complement(8769..9812)
                     /gene="cpoA"
                     /locus_tag="ACL719_001033"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGS0699023.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(9927..10256)
                     /locus_tag="ACL719_001034"
     CDS             complement(9927..10256)
                     /locus_tag="ACL719_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS0699024.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(10270..11379)
                     /gene="rpoD"
                     /locus_tag="ACL719_001035"
     CDS             complement(10270..11379)
                     /gene="rpoD"
                     /locus_tag="ACL719_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGS0699025.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(11382..13142)
                     /gene="dnaG"
                     /locus_tag="ACL719_001036"
     CDS             complement(11382..13142)
                     /gene="dnaG"
                     /locus_tag="ACL719_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGS0699026.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(13232..13990)
                     /locus_tag="ACL719_001037"
     CDS             complement(13232..13990)
                     /locus_tag="ACL719_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699027.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(13987..14853)
                     /locus_tag="ACL719_001038"
     CDS             complement(13987..14853)
                     /locus_tag="ACL719_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699028.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMDLMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(14922..15926)
                     /gene="trpX"
                     /locus_tag="ACL719_001039"
     CDS             complement(14922..15926)
                     /gene="trpX"
                     /locus_tag="ACL719_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0699029.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(16039..16256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16339..19035)
                     /gene="ppc"
                     /locus_tag="ACL719_001040"
     CDS             complement(16339..19035)
                     /gene="ppc"
                     /locus_tag="ACL719_001040"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGS0699030.1"
                     /translation="MSLQKLENYSNKSVVKEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWHNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFDSSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(19055..20284)
                     /locus_tag="ACL719_001041"
     CDS             complement(19055..20284)
                     /locus_tag="ACL719_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS0699031.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(20440..20742)
                     /locus_tag="ACL719_001042"
                     /pseudo
     CDS             complement(20440..20742)
                     /locus_tag="ACL719_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(21190..21411)
                     /locus_tag="ACL719_001043"
     CDS             complement(21190..21411)
                     /locus_tag="ACL719_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0699032.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADI
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
     gene            complement(22091..23302)
                     /locus_tag="ACL719_001044"
                     /pseudo
     CDS             complement(22091..23302)
                     /locus_tag="ACL719_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(23304..24548)
                     /locus_tag="ACL719_001045"
     CDS             complement(23304..24548)
                     /locus_tag="ACL719_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HGS0699033.1"
                     /translation="MHIVLFRYSARVVKLKYLYNRNVVIIAPEVQRKKYEDYLAEGNN
                     LPKVIFLEDFILPNIILEIRRISQNTEVESITTLSEEDMDIVGFLNDHFVEKNTHSVS
                     NLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRVFWNNCKSSAAILKPRNEAGSNGIK
                     KVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGYSIGSEIKRFYVHEYEELLFNTLSS
                     SGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNVFSINSEITPFHFEWFLELSTGKIV
                     FCEVGKRFGGGDIPQLIEDSHGVDVIKEYWDILTNSQGLKKINFSKVISRPANISATF
                     ALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYYKVSENVVENSMLVQFNCKNSCEFE
                     KRLDELKHISQKFKYVITDSGE"
     gene            complement(24572..>25225)
                     /locus_tag="ACL719_001046"
     CDS             complement(24572..>25225)
                     /locus_tag="ACL719_001046"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGS0699034.1"
                     /translation="SSATVYESTPIMPFYETNPLKASNPYGRTKQYIEVLLNDLFISN
                     SNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLNIFGVDYSTP
                     DGTCIRDYVHVNDLVYGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEILHSLESVIG
                     SYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQKHPNGYDD"
BASE COUNT         7730 a         5486 c         4161 g         7848 t
ORIGIN      
        1 tttcgcagga agtctaatct aaaaaaggta aatttaagat tggcaatacg aacgttaaac
       61 agaattccat ttctcttttt agaatcaaaa cgacaatatt gtggaatctc tacaggaaca
      121 atagactgcg acactaaaat atcctctctt agttttactt tactaatagt atataggagg
      181 agagccctga atcctagacc ctttgttatg atgcgcttac aaacagagtt ggaaaaaatc
      241 ttaaaagcag aaaaacgcat gatatcagcc cctaacactt gatacaatgc gttttattat
      301 gaaaagatct actggatttt ttatcaaaat aaacttccag tagtcttttc actttttatt
      361 aataagttcc ttcttcacct tggctagtca agataacagg tccatctttc gtaatgacaa
      421 attggtgttc atactgacat gacaatccac cgtcaatggt cttatgcgcc caaccagttt
      481 tcatatctgt atcaatttcc caatcgcctg tattgatcat tggttcaatg gttaagacca
      541 ttccttcacg aagacggagt ccacgacctg caataccata gttaggaacc attggttctt
      601 cgtgcatagt tgggccaaca ccatgaccaa ccaaatcacg cactacaccg taaccacgac
      661 tttcagcgta ttcttgaata gccgcaccga tatcaccgat acgatttcca acaacagctt
      721 gctcaatacc cttgtacata gcttctttgg ttacatccat caagtttttg acttcttcgg
      781 acggtgtacc aacagcataa gcccaacatg agtctgctaa accaccagaa tagctctgag
      841 tgtatttttt catttgttca acattgttga agtttaattt tgagacattt aggtcagatt
      901 tagcaatggg acctcccaaa accatatcaa ctttgagcaa atcaccatct ttcaagatat
      961 aatgacgagg gaaagcgtga gccacttcat cgttaagaga gcaacaggta gcataaggat
     1021 agtccatcat ggcaccgtca accccaatct gaagtggaag gaaattttct tctttacaac
     1081 gacggcggac atattcttca acttcccaca tatctacgcc tggcttaatc aaatcacgta
     1141 agcctatatg aatacttgct agaaaatcac cagccttgtc catagcttcg atttcacgag
     1201 ctgattttaa tgttatcatc ttttctccta gtttctaatt aattttaaca gtcacatttg
     1261 cttttgaaac aatctggtga ccatgataaa tatcgtaatc aattatagct gaccgtctcg
     1321 tatgatgaat aatccgtgcc tgaatgcgca atatatcatc tatctgaaca gcctgcaaaa
     1381 agtagatcag catctgctcg ataatgagat tgcgaccact attaacaact aaatcttggg
     1441 tcatgtgggt cagaatttct gccaatacac cattagccaa aactccattt ttttctagca
     1501 taaagggttc cactgtaatg actacttcat catggtgata agagagcttt tgtccaatct
     1561 gctcagaaaa agttggtaga gccgaaactt gggaacggct catcttctcc atgacatctc
     1621 gtcgcgtcac aacgccaagc aaagtttgat tgcttcgaac aactggtacc atttcaaagt
     1681 cttctgcgat catccgttga ctcacattgg caatatttgt cgataatcca accaaaaata
     1741 gactacgaga cataacctta tcaattgtcg tgcttggtga tttatcacca gcgtctctca
     1801 tggttacaac accaacaacg acctgatgtt gattgataac agggaaacgg ctgctacgat
     1861 tcttacgaac caagtccaaa taatctttaa ctgtatctgt ctctcttaga aaaccatact
     1921 catgactagg gcgataaagt ttctcaactg tcagaatatc agtcttgatt tggacatttg
     1981 acaaggcttt attgatcatg gtcgcgacgg taaaggtatc atgcttactt cttagaacag
     2041 gaatcccttt ttgattggcc agtttaagca catcatcatg aacctgaaat ccccctgtaa
     2101 ccagaactgc attttcattt tccaaggcta gcaactgaat acgggttcgg tctccgacaa
     2161 tcaagagccc cccatcatga aggtaagaca agatattttg ttcagtcatg gcaccaattg
     2221 aaaacttact aaactctctc tctaaacctt cttgcccagc cagaacctca gaagaagtca
     2281 cttctgcaat ttcagcaaac gttaatctct ctatagcaac tttctgggat ttaacacgaa
     2341 ttgttccact tctaggacgg gtctccacaa ttccacggtt ttcagcttct ttaatagccc
     2401 gataggctgt tccatcacta actcctagat gattcgaaat gctacgaaca ctgacccttt
     2461 tacctactgg taattcctcc aaatagctta gaatttcctg atgcttactc attgaactct
     2521 ccttttacat accgaaattc aagataatcc cgcatttttc ttgtgtaacg atcacttccc
     2581 ttaaactgac ggaacaaact aaatccagac ttaagagcaa ctctttggct agctttattt
     2641 tcaaggtggg taatgataaa taattgtttt aagccaaatt cctcaaaaga aagctgacaa
     2701 atttttctaa caacctctgt cataaatcct tgcgaccaag catcttttct caaaaaatag
     2761 ccaagctcag cttctttttt gatttcatct aacttctcaa atttaataga accaatcatt
     2821 tgttgatttt tctggtcaca aattgcccac actcccaaag gggacttcat aaagtaattg
     2881 gccagtgcat attgactttc ttccagactt gcctgcgttg ggaaaataaa ttgaagattt
     2941 tctggatttg aagctatctc gcggaagtcc tgactatcac taaaaaagaa aggacgcaaa
     3001 tacaagcgat ccgtttcaaa aaaagaaaac attgctaatt tggtccaaat attcataaac
     3061 acctctacgg tttaatcctc aacaagtcta atatccgctc ctaaattacg taatttttcg
     3121 ataatatcag aataaccacg taagataaac tcgatattgg taatttcagt tttaccttca
     3181 gccataagcc cagcaatgac tagtgcagcc ccagctctta agtcggtcgc tttaacactg
     3241 gtcccacgta aatcacgtcc acccgtgtac aaaatatgac catttgttgt cgaaatatcc
     3301 gcatccatct ttgctagttc aaaaacatga tttacacgtt tttcgtaaat cgtatcgaca
     3361 attgtaccac gaccattcgc tcttagtaaa agaggggtaa gcggttgttg caaatcagtt
     3421 gcaaagcctg ggtaaggagc tgtcttaata ttgattgctt tcaaattaga ctgttcctcg
     3481 acaaaaatgc tgtcttcaga tacagtcatt ctcactccca tttcttccaa cttagcaata
     3541 aacccttcca ggtgttcgta aagaacatta tttatacgaa ttcctttacc aactgcagca
     3601 gctaaagata tatatgttcc agcttcaatg cggtctggaa tcacctgatg acgtgtccca
     3661 tgtaatcttt caacaccatc aataatgatg atattagttc ctgccccacg gatatgggca
     3721 cccatattat tcaagagagt agctacatca ataatctcag gttcacgagc tgcattttca
     3781 ataatagtac gaccatttgc tttaaccgca gcaatcatcg tattaatcgt tgctcccaca
     3841 ctaaccgtat ccatgtaaat acttacacca tgaagtcctg tatctttagc agataacttc
     3901 atgttatctc cctcgtagct agcagtggca cccatagctt caaacgcctt aaggtgtaag
     3961 tcaatcggac gaggaccaag atcacatcct cccggtagac caactgtcgc ttcaccaaaa
     4021 cggcctaaga ggctcccata aaaatagtaa gatgcacgaa gactgttaat tttaccataa
     4081 ggcattggaa tattttgaac acctcttgga tcaatctcca agacatcgtc ataacgctta
     4141 acagtagctc ccatcaattc catgatttcg acaagactgg ctacatccga aatatctgga
     4201 acacaatcca aagtcaccac atcatcagcc aatataatag ctggaattaa ggccacaaca
     4261 ctatttttag caccactaat agtaatttca ccttgcagtg gtaatccacc attgataaca
     4321 atttttctca ttctaagttt tcaatactct ttcaagattt ctaataaggt cttcaaaata
     4381 aagtatatca taaatttatc cttttttcca tccttttcaa ttttattggc taaatcagag
     4441 ttgacgagtt agtaactgaa ttactaatac tcaataaaaa tcaaagatca aactaggaaa
     4501 ctagccgcag gttgctcaaa acactgtttt gagattgtag atggaactga cgtagtcagt
     4561 atcctacaca tacggcaagg cgatattgac gtgatttgaa gagattttcg aagagtataa
     4621 tctttctcga atcgctattg cccctttttt atcccctaaa actgagtagg agctaagact
     4681 agatctctta tctaagtatc ggaatacggc tgttcaacta aattttagca agaacttctc
     4741 ccccttctct cagttcacag aagcgtttta tacataccgt cctatcagac aatctatttt
     4801 aaagataacc gtgactcagg atcacttcta tctgtcgttt tctctttata gtgatagacc
     4861 tcacgggata agtcgtacat ctttcctcgt gtatacaaga tatgagggcg ttaaaaggtt
     4921 acagtgaaaa tagaaaatct tagtacgaat tcaattactc tcgtgattta cgcatatagg
     4981 ttacgactac ctttttggac tttagaattt gaggccctct tatccgctat atacgcctta
     5041 ttatcacgtt cctgttcgta gagccacgat ttcgctatcc cttcttctga caattcttat
     5101 ttccagcgtc agacttaaaa cggtctatcc ccagaccatt ttaatctgct acctcacgat
     5161 agtcaggctt gggaatcgct atttgactcg ccgagaactt tgacatcaga tatactataa
     5221 caaattgaaa taagaatcaa acaatttaat tttgaaattc aaatcaattt ataacaatat
     5281 agttgattga aactagaata gtacatctct gcttctaaaa cattgttaga aatcgatttt
     5341 actgtcctga tcgatttgtc ctgttctcat ttcattttac tatattttag aagatatgct
     5401 gtgctattcc agattcagtt tgctatacat gcctgtcgta ccacaaaatc ccgtgtctga
     5461 tgaggcacgg gattttttag cttctttact gagatggatt ttccttttta gttcgtgcca
     5521 atctcaaggc acgatttgga ttgagacgat taaatagttc ttccaatttc ttttcattcc
     5581 aaacgtctgc ggcggaaaca aaattaccat ccgcatctcg gaaagatatc ggtttatctc
     5641 ccataccagt ctcctagtct acaaattcaa actcaaattt accaatgcgg accaaatccc
     5701 catctttagc tccacgcgca cgaagggctt catcaacccc cataccacga agctgacggg
     5761 caaatttcat gacagattca tcacgatcaa agttggtcat attaaagagt ttcatgagtt
     5821 tttcaccaga aagtacccat gtcgcatcgt catcacgact aatttcaaag gctttttctt
     5881 cttcgtcaaa tccatagtaa gcttcttctt ccatatcgga ctcgtcgtag agcaaaaatt
     5941 ctggtgtctt gtctaacaat tcagctgtag catctaaaag tgttgccaga ccttgcttgg
     6001 tcaatccaga aattgggaag atagctggta actcttcaaa ttcatcataa ttttcagcca
     6061 attttttctt aaagtcttca agattttcct gactctcagg catgtccatc ttattagcta
     6121 caataatctg tggacgctcc atgaggcgaa gattgtaaga ctccagctct ttattgatag
     6181 ctaggtagtc ctcatatgga tcacggcctt cgctagctga catatcaatg atgtgaagga
     6241 taacacgtgt acgctcgatg tgacggagga actgagttcc caaaccaaca ccttgactag
     6301 ccccttcaat caaacctggc aagtcggcta ctgcaaagga ttcacctgat tgggtgcgaa
     6361 ccatacctaa atttggtaca atagtggtaa agtggtaggc accaatttta ggcttagctg
     6421 aggtaataac acttaaaagt gttgacttcc ctacagatgg gaatcctact aaaccgacat
     6481 ctgccaagat ttttagttcc aattgtaact cacgttcctg acctggttct ccattttcag
     6541 agatttccgg tgcaggattt tttggtgtcg cgaaacgaat atttccacgt ccaccacgac
     6601 caccgtgggc aacgataaat tcttgcccat gttcaatcaa atctgttaaa accttgccag
     6661 tctccgcatc acgaacagtc gtaccttgtg gtactcgaac tctaaggtcc tcagcaccac
     6721 gaccatgcat ccctttggtc atcccttttt caccagaatc agccttgaaa tgacgattgt
     6781 agcggaaatc catcaaggta cgtagtcctt cgtctacaac gaagaccaca ttgcctccac
     6841 gaccaccatc accaccccaa gggcctccat tagggacata tttttcacga cgaaaggcaa
     6901 ccataccatc gccaccatta ccagccttga ccttaatctt agctgtatct aaaaacatac
     6961 tcattatttc ttctcacttt aaaaaagggc tgggaaatcc cagtcactaa attttcttga
     7021 atctatttta tagattactg agggcaccaa ttgcagttgc aaaaattccc aataaacttg
     7081 ctactagcat gataatcacg ataaacaagg ttattttctc aaacatagtt tttttacgat
     7141 ttccattatc tccaaatgcc atttttctct ccttcgttac attctatttt ctattatctt
     7201 agcatgaatt gagctagttt tcaatagtca gttgctactg gtgcgataat ccataaaata
     7261 atgtaagcta caattccagc tccgtaacag caagtaagaa caccccaaat gacttgaacg
     7321 atagtcggat ccatatccag ataatgggcc accccagcac aaacacccgc aatcttttta
     7381 tcacgaccgc ttctcattaa ttgttttttt catagctgtt cctctttcta ttcttcatgg
     7441 tctttccaat agtctctcat actgatcaac tgtgtttttg aagccttcaa catgcgtcta
     7501 gatcctttta caggtacagc aaccacctgt tgctgaagat acaaaactgt cttaaagata
     7561 cgctgactga ccgtatcatc tttagccaaa tctttctgga agttatttga aataatggca
     7621 tccagataaa actcatgcac tcgtttccca aagttctcag ctgaaatctc atacaatttc
     7681 tctgataagg tatgctcgtt catgtctggt gttgcaatca gggcttccaa aatagcacca
     7741 gccaaatcat gttctccata gtacaaggtt ccaaacattt tatcactgat gaggttgttc
     7801 aaataaggat ttccgtgagc aatgacaggt gttccactgg ctaagctttc caagtaggtc
     7861 aaaccttgcg tttcgcttgt cgatgccgaa atgaagaaat ccgccgcttt atagtaaaga
     7921 gccgtctcac taggagcaat catccctgta aagatgactg agtcttgaat ctctaggttc
     7981 tgggcttgct ctttgaggtc attcagataa gggccatccc cagctactac cagtttaacc
     8041 ttgtcttcct ctttcagaac atcagcaaag gctgctaaaa ctgcttgaat atttttttca
     8101 taggagattc tcgaaagact aagcaacgtc ttttcaccat cttgaatccc tagtttacta
     8161 cgcagttctt tcaaattttc ctgcttgatt tccggacgct caaacttggc taattcaatc
     8221 ccagtaggaa tgacccgttt ttcaaccttg accttataat cagatagcaa gtcacggaca
     8281 atctcactag ggcaaataac cccatccaca tcatgcagga aacctctaac cagatacttg
     8341 accatactcg accggatcaa catcccctta gcaatataat ggacatagtc ttcatactgg
     8401 gtgtgatagg tatggatgac tggaattttc aattcacgcg caatccaaat ccccaacagg
     8461 ccaagagaaa attctgtctg agtatggata atatctagct gatactgttt agcaatttca
     8521 agtgccttgc taaaacctcg gtaggcaaag cgacgatcct taaaagcaaa gaaaggaaca
     8581 cttggaatgc ggataatttg ccaatcttcg tagcgattga catccttatc tgtcgtcgta
     8641 aagataaaaa cagcatgtcc ctgcttttca agttctgttt tcaaggttcg aatactggtc
     8701 gcaacaccag aaacctgagg aaaataggta tctgtaaata aaccaattcg catagattac
     8761 ctcacttttt actttctccc taaagcggct tgtttctcat aaaagtccaa ccagatttgt
     8821 aacagatgct cttcagaata ctctctggaa atattcttgg ccttttcttt gagatctttt
     8881 aagacagcag gatttgcttg atattccaaa atagcctctt tcatctcttc tctacccgct
     8941 gtcgcccgat aatttccctc caaaatcacc ttatagagat ctaaatcacg caacataata
     9001 ggagcctcac aactcgcagc ttctaaaata gtcataggaa agagctcatt gtaactaggc
     9061 aacaagaaaa gatccgctag agcatacaat tcgcgcatcc gctctggcga tacaatgcct
     9121 ggaaaaatca aatttttagg gggattttcc ataattttct tatagtgttc ataaccatct
     9181 gtcataccac caaaagagaa gccaccagcc cagataaagg taatctgagg caattcctca
     9241 gccagacgga taaagtcatc aatcccttta cgtttctgaa cttgcccagc acctactacg
     9301 ataaactgat tgtcactaag accaagatct gtgcgcagtc tgactacctc ttcttgtggt
     9361 agaggatgcc atttttcctt gttgacaaag ttaggaatat aggtcacttt ttcacgtgga
     9421 ataccagctg ctaccaaatc ctcaataaac ataggattga ccacaaccaa gtgctccatc
     9481 cggttgtaaa aagaaaatac atagcgtttc acaattccct ttaagaaaaa tggaattttc
     9541 aaacttcctt caagtgtagc tggcaagaaa tgcacatagc caatctttct ccctgagcgt
     9601 ttcttttgga aggttgataa ataataggga aaatcaatcg tatgaaagtg agtcacatct
     9661 gcctcgattg gaagattttc tgtaacaatc aattggtcct tggcagcacg gtgaagaaga
     9721 cgaactaatt cacggtaagc acctgaaact ccctgtcctg ctactttctc acttgaactc
     9781 aacatattga tgcgtaattt ctttttctcc ataactacta ttatatcatt ttctttgaat
     9841 aaaatcagca aaagaaagga gagactagag gaaaagaggg gaaatttcct cgcttttcca
     9901 acagtctctc acatgctttt atatgtttac ttaatgccta gggcaatgcg ggcatagcga
     9961 ctcatttttt caactgtcca ggctggatac cagactaatt taacctcagt atccgttact
    10021 tctggaacct ctatcatagc atcataaatc tggtctgtca aaaggtctgc taagggacaa
    10081 cccatagttg tcaaagtcat gtcaatctct gtttgccctg tgtcaccgtc aaaacgaatc
    10141 tcatagatca aaccaagatt gacaatatcg attcccaact cagggtcgat gacttcttcc
    10201 aaggctgtta aaatccgtgt tttgatgttt tcaatttgct cttctgtata agccatattt
    10261 tcctcactct tagtcttcaa taaaatcacg aagcggtttg ctgcggcttg gttggcgtag
    10321 ttttctcaaa gcctttgctt caatctgacg gatacgctca cgagttacgt taaagacttt
    10381 ccccacatct tcaagtgtgc gcatttttcc atcatctagt ccaaaacgta gacgcagaac
    10441 attttcttca cggtctgtaa gagtatctaa gatttcatcc aattgctcac gcaagacgat
    10501 acgagtcgta taatccactg gattttcaat tacttcatct tcgataaagt ctccaaggtg
    10561 gctatcgtcc tcttcaccga taggagtttc aagagatact ggttcttggg caatcttcaa
    10621 gatttcacga accttatcag gtgtcatatc cattcgttca gcaatctgtt ctggtgtcgg
    10681 atcttgcccc aattcttgaa ggagattccg ctgttcacga accaatttat tgatagtttc
    10741 aaccatgtga actgggatac ggatggtacg agcttgatcc gcaatagcac gagtgatagc
    10801 ctgacgaatc caccaagttg cataagttga aaacttgaac cctttagaat agtcaaactt
    10861 gtcaaccgcc ttcatcaagc ccatatttcc ttcttgaatc aagtcaagga actgcatgcc
    10921 acgaccgaca tagcgtttgg caatagaaac aaccaaacga agattggctt ccgcaagacg
    10981 ttgtttggct tcgatatcgc cagcttcaac agccagtgcc aactctttct cctcttcatt
    11041 ggtcaagaga ggaacgaccc ctatttcttt caagtacata cggacagggt cattgacctt
    11101 agcagaagtt gacccaatca agtcctcatc gctgagttct ggttcttctt cattgctaag
    11161 aacacgcgca cttggatttc cttcgttatc tgtgatagaa atgcctgcat cctgaatccg
    11221 ttgcaagaga tcttcaatcc catcagcgtc caaggtaaaa ggaataacca gacttgcatt
    11281 gatttcatca tctgttgctg tccctttttg cttatgatta cggataaatt ctgctacctg
    11341 tacgtcaaat gttgttactt ctttttgttt tgttgccatt attactccat tcttctcttt
    11401 tgggaaatta aacgttccaa ttcttctagg gctgtatctg tatctcctac atggctagct
    11461 tcctgcacct tctttttgat tctcatattg tcctgattca agagagcctt gtttcgagtc
    11521 atctctactt cactaagttc ctgcggcgat atctcagcag gcaaatcctg agctaaaact
    11581 tggtaccaag ctctttcaac ttcctctgtc tgctctgcta aaacttctgg aggaagattt
    11641 ccatactggc caagcaagtc atataagacc tgaaattcag gtgtagcaaa tgcaaagtct
    11701 tctcgcaaac ggtaatcgtt caaaacaaga ggggattcca tcatccgata gagtagatgg
    11761 gcttctgccc tcataatagc cgataactgc ttggtgatag gcatggtgat tggcgtcggt
    11821 ctggaaattc cttccatgcg attctgcctt tgcacctgac gactctcatt aacaatctgc
    11881 tcaatctggg tataatcaaa ggacgccaga ctgtcagcta aaatatgaat atagctgttt
    11941 tgagcagcga tggacttttc ttgaacaatc aagggagcta ttttttcaat aaactcaatc
    12001 tgagcctgca gattttcact gttttcaggt ttgtactgat gaatgtagaa ctcaatcgga
    12061 ctaatacgag tttttgttaa tagataggcc aagtcttctg gaccattttt ttgtagatac
    12121 tcatcaggat ccaagttatc aggcatgcta acgatttgca caggcatatc accaatttca
    12181 tccaatgctt tcaatatcgc ggcttgccca gccttatctc catcgtaaac aagaaccaat
    12241 ttcttggtta accttttcag atgctcaaca tgctctcgac tcaaggccgt tcccatcgac
    12301 gccacagcat tttcgattcc agcccgatag gctgcaataa catccatgaa tccttccatc
    12361 aggtaaatct cactagcttt tccagaagat ctttttgccc tatccatatg atataattcg
    12421 taacttttgt taaaaattgc agtcgatcgg ctgtttttat acttagaagt ttgtgaatcc
    12481 gttttttgcc agatacgacc tgagaaggca atgacctttc cttggtcatt tgtcagggga
    12541 aacataatgc gattgtgaaa ggtgtctaca aattgattgg catccgagag ataaaacagg
    12601 cctgaatcca gtaaatcctc ttcacgatac tgatcagaca aacgttgata gagatagttt
    12661 cgttctggag gtgctaaacc aatccaaaaa tgtttaagca cttcatctgt caaaccccgc
    12721 tgataaaggt aatttctggc ctcttctccc atagtcgttg tcatgagaat agcatggtaa
    12781 aatttggctg catcttcgtg catatcataa agagcttggt gaggcgaggc tggcttctgt
    12841 tcactataaa gcggtttttc aacctcaatc ccgacacgct gacctaagat ttggacagcc
    12901 tccataaagg taaccccttg gtactcctcg atgaatttaa agacatcacc tgagcgacca
    12961 caaccaaaac agtggtaaaa ctgcttgtcc tctacaacgt tgaaagaagg tgttttttca
    13021 ccatgaaaag gacagagccc tagatagttc cgtcctgcct tttgtaaaga aatcacatct
    13081 cctatgactt ccacaatgtt ggcattgttt ttgatttctt caatgacttg tttgtcaacc
    13141 atacacaata cctccatgtt atcatagttt actttatata gtatacttta tttcagaaaa
    13201 aaagtaaacc atttcactca ttttccctac tttattcaaa gagttgataa taatcagaga
    13261 ttttcatttt tgctttttct tcttggttta aatcttggat aatccgtcct tctttcatga
    13321 caatcaagcg attgccgtat ttgagagcat cttccatatg atgagtaatc ataagggctg
    13381 ttagctgatc tttcttaaca aattcatctg tcaattccat caaagcaaca ctagtctttg
    13441 gatccagggc agcagtatgc tcatctaaca ggagtaattc aggtcgcttc aaggttgcca
    13501 tcaagagact caaagcctgt ctttgtccac ctgataagaa ctcaatcggt gtattcaagt
    13561 gtttctcaag accatttcct actttttcaa tggttgcctg aaattcatcc ttatagctag
    13621 tcaagcgtct tggtaacaat ccacgctttt caccacgaaa cttggcgatt aaaagatttt
    13681 cagcgaccgt catacgggga gctgtcccca tctttggatc ttggaagaca cgagacaggt
    13741 acttggcacg cttctcgggt gaaaacttag tgagatcttc acctaaaata cggatagttc
    13801 cactagttag tgataaggtc cctgctatag tgttaaagag agttgatttt ccagcaccat
    13861 ttccgcccaa aatcgtgata aagtcccgtt caaaaatttc taaggaaaca tcatttaaaa
    13921 taatcttttc ttcatcaaag ccatttttaa cgattttggt tgcatttttt aattctacaa
    13981 ttgctgtcat ttgcttaact tggctccttt caagattgtt tgcttaaatg ttggaatcat
    14041 gaggcagact gctaaaatca aagcactgta taaacgaagg taacttgtat taaagccaag
    14101 tgcgataact gcccacacta aaaattgata agcgatagaa cctacaacga tagtaaccaa
    14161 acgctctgcc aagctcaaac tcttgaaaat aacttctcca ataatcaaac ttgcaagccc
    14221 cacaacgata accccgatcc ctcgagacac atcggcatag ccttcttgct gagcaatgag
    14281 ggcacctgca agggcaatca caccatttga taagaccaag cccatgagat ccatgcgtcc
    14341 agtatgaatc ccgaaacttc tagccatatc aggattatcc cctgtagcaa tataggcttg
    14401 tccgagttta gtgtccaaga aaaagagcat gagagcaata acaatgctca caaagatgag
    14461 acctgtcaag agttgattca aatccgaatc aaaaggcaaa acatcctgaa tttgcttggt
    14521 tccaagcagg cctaaatttg cacgtcccat aatcaagagc atgattgagt gacaagaagt
    14581 catcaccaaa atccctgaga gcaaggttgg gatcttccct tttgtataaa gaaggcctgc
    14641 tgccattcca gccaaacaac ctgctcctac agcaacaagt gtcgctaaaa atggattcac
    14701 gcctttggtt atcaaagtga cagcaacagc tcccccaaga gggaaggaac cttctgtcgt
    14761 catatctgga aagtttaaaa tcctaaatgt cataaagatt cccagaccta gaatagccca
    14821 gacaaatcct tgagaaataa tggaaacaat catattttat ttaatccttt ctatattcat
    14881 ctttttaaaa aatgggaaga gtctcctcct ccctacctta tttattcgat gacttgtcct
    14941 gcttctttga gaacagactc aggaatagta atacctagtt cttgtgctat ttttttattg
    15001 ataactgact taccagttga aaagacattg actggggtat cggctggttt tgcacctttc
    15061 aagacttgca caatcatttt acctgttgcc acaccaaggt catgttggtc aattacaact
    15121 gatgccaaac cacctacttc taccatagct gtcgcactgg gataaattgg tttcttagaa
    15181 ctttgattgc tagagacaac cgttggaaat cctgatgcaa tggtgttatc aattggaacc
    15241 caaatagcat ctaccttgct agtcataaca gtgacagttg aggcaatttc atttgttgaa
    15301 ggaactgcaa atgtttccac tgtcagacct gctttttcag cataagcctt aaattcttcg
    15361 acctgtgttt ttgaattgtc ttcgctactt gagtaaagag ctccgattgt tttcacattc
    15421 ggtgtcagag ccttgatgag ttcaacttgt tgttgagctg gattgtggtc agatacccct
    15481 gtaacgttgc cacctggttt tttcaaatct ttaaccaagt tagcaccaat tgggtctgta
    15541 atagcggcca tgataaccgg taggtctttt gttgcactag ccaacccttg ggctgctggt
    15601 gttgcgatac caaccacaag gtcattccca tttgcaacca attgtttact cattgtcgca
    15661 accttacttt ggtcaccttc tgagttcata aaatcaattt taacttgatc atctttatat
    15721 ccttcttctg caagtccatc ttggatccct ttataaatca aatcaaggga tggatggctc
    15781 acaaattgaa gcacaccaat cttggttatt ttcttctcat ccttattatt ctgaacttct
    15841 gatttattca ttgaagaata aatcaagctt cctgctacta agactgctaa tgcagcaata
    15901 attccaatta aacgtttatt tttcattctg tttctccttt ttatttcctt taacatattt
    15961 cacttatcta aacaagcgac gccattgttt tctttccata ctaacctcca tcaaaaaaat
    16021 cctcacacaa aaaacttgtg tgaggacgtc gatgcgcggt accacctcaa ttatagggaa
    16081 tttccctatc gctcagtctc gcaataacga gatgcactgt aaggtgtgct taccgaattt
    16141 ttataatttc aaattctaaa tagcattcag cccaattttc atcattgcca cttatctgtt
    16201 ttcagctacc acagactctc taaaaagttt gtatgattac ttttctgaat ggttaaatta
    16261 tatcattttt aaactacttg tcaatagtct attagaattt ttttgatatt cacttttagc
    16321 attcactctt gaaaattatc aacctgaatt acgcaatcct gtcgcaattc cgttgatggt
    16381 gatatgaatc aatcgttctt gatcactgga caattctcca cgacgttggc gtttaatcaa
    16441 ctctaactga atatagttga gaatattaaa gtaaggcata cggtaatcca gactagcttt
    16501 tagatatgga ttgtcagcta agagttcgtc atgtccttca atagccaaga taacgttctt
    16561 agtaacttgc cattcattta aaatagtctc atagatggcc ttaacttgct cgtcttcaca
    16621 aagtttagca tattcaaaag caatattcat atttgatttt gacaaaacca tatcaacatt
    16681 tgaaagaagc gattggaaga aaggccaatt ttggtacata tctcgtaaga tagcaatatt
    16741 ctctggattt ttattgataa attccttgaa gcttgaacca accccgtacc atccagggaa
    16801 cataacacga ctctgtgacc atgagaatac ccaagggatg gcacgcaatc caccgatttc
    16861 agtaatagtc ttacgagcgg ctggacgaga accaatatta aaacttgaaa tagccttgat
    16921 tggacttgaa tcgaagaaat aatcatagaa atgctcatta ccaaagacca aatcacggta
    16981 gatatcgtaa ctacggtcca ctacttgatc cataatggct tcataacgat ttggggtatt
    17041 ggtatcgctc ttcttctgag taatcatacg gttaatagct gccgatacta gcatttcaag
    17101 gttatagtaa gcggcgtctt tgttaccgta tttattccca attacttcac cctgctccgt
    17161 caagcggata cgatccttga tagacttgag cggttgagat gtaatggctt cataggttgg
    17221 cccaccacca cgaccgacag taccaccacg accatggaag aaggtaacct taacgccaaa
    17281 ttcatctcca atagcagtca attgttgttg agccttgtag agggtccaac atgatgacaa
    17341 gtaaccgcca tctttattac tgtcagagta gccaagcatg atttcttggt agttatttcg
    17401 tgagtcaatc cattttttgg caaggctaag agaaagatat tttctcattg tttcctctga
    17461 atgatccaag tcttcaattg tttcaaaaag gggaacaatc tgaacacgcg ccctttccgt
    17521 atccaccagt cctacttctt ttaacagaat agctaattct agcatatcag aaaggctggt
    17581 tgcatgtgaa atgatggtct gacggatgac atcatctccc aacttatctt tcaaaacacg
    17641 agccgtctta aaaatagcta attcttttgc taataattct gatttttctg cgtgagtcgc
    17701 agaaagaatt cggggatctt cttctaattc tttcaagaga aggtcacact tttcttcttc
    17761 gctcaactcg ctataacgag aatgaattcc tgctgatttc aagagttctg ccacacaggc
    17821 ttcatagacg ctagagtctt gtcgcatatc aattgacgct aagtaaaaac caaatatctc
    17881 tactgcctgc aataattcca caaaatcacc tgaaatcaag gactcaccct tattttctag
    17941 tagagaatct cgaatggcaa tcaaatcctt gtagaaatca ttggcggttt cataacgagt
    18001 cccaacttct tcatcctcaa tcagataggt tttagttgcc tgaatttttg attgaatatc
    18061 aaacaaggca cgacggtaaa gctctttttc gcggtaaata gagttatcct tggattgacg
    18121 agccatttct ctgacttgct tgctgacgtt gacaatgcta gttgaaagag aaaattcacg
    18181 ataaagttgg taaatctttt tatcatagta gttcatgatg acttcacact gagtgagtgc
    18241 agactgcttc aaggtctttg ctgtaacaaa tggatttcca tcacggtcac cacctatcca
    18301 catacccatg gtgattggtt tagcctgttt taaattcaga ccatgcgctt gcgctaagcg
    18361 cttatactcc gtcgtcaaat gagggacagc tttcaaaaag gagctgttgt aatattccat
    18421 agcattcgtg atttcgttag tcacttttaa ttttttctca cgaatcatgt ctgtctgcat
    18481 gataatttcg atgtaacgac gcaaatcatt gtgccattta tctttattga ttaaccccaa
    18541 cttaacatca cggtatttac gcaaaagact atgaatatga tttgttaaat ccaacatact
    18601 tttgcgttgc acttgtgttg gatgggctgt caaaacaggg acaacattca agtgttctag
    18661 gatctcaacg gcattttcct tttctgctac caatttaatc gttgtagata atttacctaa
    18721 atagtcctga tcaatattat tttgatgatt gatttcataa gctaaatcca catcctctga
    18781 aatattaatc aaaagaggta agatagagaa atagcgtgaa atatagacca tttcatcatt
    18841 tgataagcta gtcactagac ggtttagacc ttgataatct tcctgcgttg ataattcttt
    18901 caactgtatt attttttcaa aggtctctgg ggcaagcata tttttagtaa tatcttccag
    18961 taattctgtt agaatcaaga cttcttcttt cacaacactt ttattactat aattttctaa
    19021 tttttgaaga gacataaact atcctttctt tatcctactt caacagaagg ttcattggtt
    19081 gattttccaa ttctcggtac aacttagcgc gtttttcact ggcatcaata tttaagacaa
    19141 aggctactgc cactgataag actagaagac tatttccacc ctgggataag aaggggaaag
    19201 tcactcctgt agatggaatc aagcccgaaa tccctccgat attgacaaat acctgaacca
    19261 acatcatccc tccgacaccg agtgcaacca tggcattgaa aggattctcc gctcggatac
    19321 cgaccaagat aatccgcaaa atcatgaaaa acaagagagc taaaataaga ctggcaccaa
    19381 caaagccaaa ttcttcaatc acgatagaaa agacaaagtc tgtatgagct tctggcaaat
    19441 aacctcgttt ttcaatcgag tttccaagac ctagaccaaa ccaaccgcca ttgaccatgg
    19501 caaaataaga attagctaac tggtgacctg catcagcacg atcggcaaaa ggattaaaaa
    19561 aggcactaaa gcgcttggct acatagccaa atactggaat ttttgaaaag gtctcaacac
    19621 cgattaggct gatagtggtc aagacaaaga cagaagtggc agatacgagc gccagaatgg
    19681 ttgaaaacca gcgataagcg attccactaa ctgtatacat aatcaaggaa accaagacta
    19741 aaatagtcgc atttcctaaa tcagggaaaa ttcccaaact tccaatcaga actaggagaa
    19801 cgaatcgcca atcattaaaa gcacggggaa gccattgatt ttgagtcaaa acttgaaaat
    19861 cataagtagc tatttcttct tgctgtttgg agaatcggtg agctaaatac caaataataa
    19921 tgatttttaa gtactcagct ggctgaatag ttactcctgc aaccgaaatc caaccgtatg
    19981 ccccgtttac ggaaatacca ataaaacgag ccaagaacaa taaaagcatt tctattaata
    20041 taactaaaat gattagtcgc tcatttctca aaaaatctag tctcaattta taaattaagg
    20101 caatcagtat caaactaaca atccaaaaga ttccttggtt tcgaaccaac tgcaaggcac
    20161 tcttgccttc ttcaattaaa atagcactgg tggtcgaata gaccacaatc aagcccaaaa
    20221 tagataaaag caagtaggga atcaagatgg aataatttaa taagtgcctc ttactaatct
    20281 tcatagcatc accactctac taggatacag ataaatctgt tccaaattcc gttttatttt
    20341 ctagttttga ttgctattat accatttttt cagaaagaaa aaaactctta ccttacaatc
    20401 cttcgaattt tactgttttt acagaattaa ggcagaattt gaaatcgtaa aaccattttg
    20461 aataagagtt gcttcaaccc attgactccg acggattaag ttgctttcgt gaatgccaaa
    20521 atcagccgca atttgttcat aagtgctcta ttctcgcatg tattgaatag ttaccataag
    20581 gagatcgtct aggattaatt tgctttttcg tccacctttt gcgcgtttac gttgataagc
    20641 tgtttttaac acagctaaca tctcttcaaa agtcgtgcgc tgaacaccta caaggatctt
    20701 gaaacggcta tcgcttaatt gtttacttgc ttcatcatcc atagttttat tgtattgtat
    20761 ttttgtttcg caggaagtct attattcctt tcgttccaag gtttttacgc tttttcaagc
    20821 aatcctacac actccacgtg atgcgtctgt ggaactcaaa aggttcgggg aaccttttga
    20881 ggattaattg cgtttctcta acaaactcac acattcaacg tggcatgact gatgaagtca
    20941 tcataagaat ttcacttgct gctctttctc ggtggcagcc tgaacggtca gaagtatcat
    21001 tgtatatcat ttgtatcatg gcatataaag tatcgctcta ttttattgat ggttttaata
    21061 tcgctcttat catggcgaac cattcaaatc tgctccacaa atgaggaaag taccattttg
    21121 gactattcaa ttgtcaaaga acaaataaaa aaaatcttcg cattttcaca ctctatgaaa
    21181 ttgaggagat tattttctaa agcgaatttt atcaggaaaa tccgtcaatc ccaataggta
    21241 gtctgtactg acatcgtaaa agtttgagag agttaccaat atatctgctg ttaacgtatg
    21301 ctcaccccgt tcaattttag cataagctga gtctgtaaac gaaagtattt tagctatttg
    21361 tttttgggtc aaatcgtgat cttccctcaa atctctcaaa cgtctgtaca tctagctttc
    21421 tcctgagaaa agtataatat ataaaactaa gaaatcattc aaagtgtcca aactggacat
    21481 tttgaactaa aattaattta ttaaacatat ctcataagca tatctattat aggtaagaca
    21541 atattaagaa agacggtcaa aatctattaa ccgtctaaag atgttataag ctattaaaaa
    21601 atcagcagta atccggtaac aaaagtcaca taattttgtt aaggaaggga ttaatgatgt
    21661 ccagattact actaaccagc aaattgaaaa atggtcaaca tgatatttct aatgtgaatc
    21721 tcttactttc agaacctaaa agttgaataa tagccacccc ctacttatca ataaaatttt
    21781 ccgttgcatt aacagatgta tatagaccac tccatggatt tcaaagcaat taactagact
    21841 ttatttgtca ataaccacaa aattagatta gaaaaatcat taaaaattaa ttggagtcct
    21901 ttgtgatgct ttttcaataa aagtaatact tctcaatatt ttaactgaac taaagaaata
    21961 gcataatggt atcttgattg tattcttcta aaaaggattg cccttcacat tcaactgtga
    22021 ttgattaaat tccaaaaata tattccaaag gcgctctaaa ttctctatat cagctatttt
    22081 ttatcatcta tcaacttcta tcatacctct tcaatagcgt gctaaatatt agcgataata
    22141 tcgtaataga tacaccaata ataaaaaaat taaaagtatt gttaggattt aaaactactg
    22201 agaaaaaaac agtacctagt ggcataaaaa gaatagctac cgtaaagatg attccaaata
    22261 ctctgccaag aaactcatta tctacgtctc tttgaacaat agagaaaaat tggatattaa
    22321 aaatggaaag aaatagacta aataatgctg gagtaaaggc taaaataatg acgttgcgga
    22381 acatataata gatcggcgct gttagcatta acatcaaacc tgaatatgct aaaaagagca
    22441 ttaaacgttt gcttgacaag gatttattga taaatccact taatatcgca ccaataaagc
    22501 caccaatcgc ttcggctgtt aaaaaggttc catatagccc acttgaaata cttccaaaca
    22561 tttgattact gtatggcagc aacaaattat aggctgctag gaaaaagtta acaagtgctg
    22621 aaagggcaat tatagtgaga atttgtttat gactatatac atacgtaaat cctattttta
    22681 aatcatcaaa gattcctctt atcgtcatat tctcttttgt atccacctcc tcatttacag
    22741 gagtaatgaa gaaaattagt gaggcagcaa ttaaaaacga taatccatct agtaataaca
    22801 caccatgtat tccaagtaac ttatataaga aaattgcaat cataggaatt gttactttaa
    22861 tcacagtact tgttgtttct agcaatgaat taagttatga tatactgtcc ttttttacaa
    22921 tttctttcgt aaaagctttg tatgatggcc ctgaaaaagc actcataaaa gccaaaatga
    22981 catttgttat gactatcgca taaaccaacc atttttcttg ggatataaat gaaagagtta
    23041 tgcaagcaat accacataag atatttgtag taataataat ttttttccgc ttgaaactat
    23101 ctgcaataac tccaccaaat aaattaaata gaacacctat catactttct aatgattgat
    23161 aaattgcaac cactgataga gatgttggat taagaccagc aagaaaggta ttattggcga
    23221 aatcaaacat gatatctccg attcgagata tcatcctact agctacaatg gatagaatat
    23281 tgtgatgttc ttctctaatc attttattct cccgaatcag taatcacata tttaaacttt
    23341 tgtgatatgt gcttaagttc atctaacctt ttctcaaatt cacaactatt tttgcaatta
    23401 aattggacca acattgaatt ttcgactaca ttttcagata ctttatagta agtatttggt
    23461 ttaataaata tgtacacttt ttttgcccaa gccaattctt tttcatcagg tacctcgata
    23521 accttacctt gctttctata taaagcaaac gttgctgaga tattagcagg tctagaaatc
    23581 actttagaaa aatttatttt tttcaatcct tgtgaattag tcaaaatatc ccaatattct
    23641 ttaattacat caactccatg actatcctca attaactggg gaatatctcc ccctccaaat
    23701 ctttttccaa cttcacaaaa gactatttta ccagttgata attcaaggaa ccattcaaaa
    23761 tgaaaagggg ttatttcaga attgatagaa aaaacattca aaactttgcg acattcttcc
    23821 aatgcttttt ctatgtattc aattttttca ctatcattat ataacgaact agtccttaca
    23881 agataatatc cactagaact taaggtattg aacaatagtt cctcatattc gtgaacataa
    23941 aatcgcttaa tctcacttcc aattgaataa ccatcacatg ttatcatgtt atcgatatca
    24001 atgtattcct ctataatata gtttttttca aaatacactg aatcaatttt ctcgttaggg
    24061 taaacttttt ttattccatt acttcctgct tcatttctag gttttaaaat tgcagcagaa
    24121 gatttacaat tattccaaaa aacacgtaaa tcattttctg attctaaaag cctaaaatac
    24181 ggttgattta ctactccaac taaaaatgaa cgcatgaagt atttgtcttt aaataataaa
    24241 ttagacacag agtgagtatt tttttctaca aaatgatcat tcaaaaaacc tactatatcc
    24301 atgtcttcct cactcaaggt tgtaatactc tcaacttctg tattttgact tatccttcta
    24361 atttctaaga tgatatttgg tagaataaaa tcttctaaaa aaatgacctt aggtagatta
    24421 tttccttcag ctagataatc ttcatatttt tttctttgaa cctcaggtgc aataattact
    24481 acatttctgt tatacagata ttttagttta acaacccgtg cagaatatct aaataatact
    24541 atatgcatgt taatcccaat cctttcaatt attagtcatc atatccattt ggatgtttct
    24601 gttgccattt ccacgtatct tggcacattt tcataatatc ataccttggc ttccatccca
    24661 gtagctcttc tgcttttgaa atatctgcaa ttgaaacgtc catatctcct gctctccttg
    24721 aagttatctt atatggaata tacgagccta tgacactctc taagctgtgt aggatttcaa
    24781 atacactaaa accaacacca cttcctaaat tgattacttc atataaacct tcatctgtat
    24841 taaaaatata ctctaatgct tttctgtgtc cgtatactaa atcgttaacg tgaacatagt
    24901 cccttataca agtcccatca ggagtactgt aatcaactcc gaatatattt aaatatggta
    24961 attttccaat tgcaacttgt gtaatgtaag gaacaagatt attcggaatt ccatttggat
    25021 cctctcctaa atcaccactt tcatgtgcac ccaatggatt aaaatacctc agacaaacaa
    25081 ttttccaatt tgaattcgaa ataaacaaat catttaaaag tacttctata tactgttttg
    25141 ttctcccata cgggtttgaa gcttttaatg gattagtctc atagaatggc atgataggag
    25201 tactttcgta tacagtagct gatga
//
LOCUS       DBERCA010000021        24611 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.021, whole genome shotgun sequence.
ACCESSION   DBERCA010000021 DBERCA010000000
VERSION     DBERCA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.021"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..457
                     /locus_tag="ACL719_001047"
     CDS             <1..457
                     /locus_tag="ACL719_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS0699035.1"
                     /translation="AIPVSTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAF
                     TEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSPDNGMMESFFGILKSE
                     MFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(500..1141)
                     /gene="plsY"
                     /locus_tag="ACL719_001048"
     CDS             complement(500..1141)
                     /gene="plsY"
                     /locus_tag="ACL719_001048"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGS0699036.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1277..3220
                     /gene="parE"
                     /locus_tag="ACL719_001049"
     CDS             1277..3220
                     /gene="parE"
                     /locus_tag="ACL719_001049"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGS0699037.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3235..3439
                     /locus_tag="ACL719_001050"
                     /pseudo
     CDS             3235..3439
                     /locus_tag="ACL719_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            3640..6111
                     /gene="parC"
                     /locus_tag="ACL719_001051"
     CDS             3640..6111
                     /gene="parC"
                     /locus_tag="ACL719_001051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGS0699038.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLV
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6243..7265
                     /locus_tag="ACL719_001052"
     CDS             6243..7265
                     /locus_tag="ACL719_001052"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGS0699039.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7321..8025
                     /locus_tag="ACL719_001053"
     CDS             7321..8025
                     /locus_tag="ACL719_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0699040.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8090..8779
                     /locus_tag="ACL719_001054"
     CDS             8090..8779
                     /locus_tag="ACL719_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGS0699041.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8776..9699
                     /locus_tag="ACL719_001055"
     CDS             8776..9699
                     /locus_tag="ACL719_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGS0699042.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9714..10358
                     /gene="pcp"
                     /locus_tag="ACL719_001056"
     CDS             9714..10358
                     /gene="pcp"
                     /locus_tag="ACL719_001056"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS0699043.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10526..10756
                     /locus_tag="ACL719_001057"
     CDS             10526..10756
                     /locus_tag="ACL719_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGS0699044.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10806..10886
                     /locus_tag="ACL719_001058"
     tRNA            10806..10886
                     /locus_tag="ACL719_001058"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10840..10842,aa:Tyr,seq:gta)
     gene            10896..10967
                     /locus_tag="ACL719_001059"
     tRNA            10896..10967
                     /locus_tag="ACL719_001059"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10928..10930,aa:Gln,seq:ttg)
     gene            11129..12331
                     /gene="rpsA"
                     /locus_tag="ACL719_001060"
     CDS             11129..12331
                     /gene="rpsA"
                     /locus_tag="ACL719_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGS0699045.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(12416..13742)
                     /locus_tag="ACL719_001061"
                     /pseudo
     CDS             complement(12416..13742)
                     /locus_tag="ACL719_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13936..14433
                     /locus_tag="ACL719_001062"
     CDS             13936..14433
                     /locus_tag="ACL719_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGS0699046.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            14433..16088
                     /gene="dnaX"
                     /locus_tag="ACL719_001063"
     CDS             14433..16088
                     /gene="dnaX"
                     /locus_tag="ACL719_001063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGS0699047.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            16116..16310
                     /locus_tag="ACL719_001064"
     CDS             16116..16310
                     /locus_tag="ACL719_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGS0699048.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            16460..17230
                     /gene="sufC"
                     /locus_tag="ACL719_001065"
     CDS             16460..17230
                     /gene="sufC"
                     /locus_tag="ACL719_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGS0699049.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            17258..18520
                     /gene="sufD"
                     /locus_tag="ACL719_001066"
     CDS             17258..18520
                     /gene="sufD"
                     /locus_tag="ACL719_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGS0699050.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKDAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            18531..19757
                     /locus_tag="ACL719_001067"
     CDS             18531..19757
                     /locus_tag="ACL719_001067"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGS0699051.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDRVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            19744..20184
                     /gene="sufU"
                     /locus_tag="ACL719_001068"
     CDS             19744..20184
                     /gene="sufU"
                     /locus_tag="ACL719_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGS0699052.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            20238..21650
                     /gene="sufB"
                     /locus_tag="ACL719_001069"
     CDS             20238..21650
                     /gene="sufB"
                     /locus_tag="ACL719_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGS0699053.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(21881..23122)
                     /gene="pbp3"
                     /locus_tag="ACL719_001070"
     CDS             complement(21881..23122)
                     /gene="pbp3"
                     /locus_tag="ACL719_001070"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGS0699054.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPIE
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIEQI
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            23225..24334
                     /gene="cozE"
                     /locus_tag="ACL719_001071"
     CDS             23225..24334
                     /gene="cozE"
                     /locus_tag="ACL719_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGS0699055.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGVFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         7461 a         4401 c         5452 g         7297 t
ORIGIN      
        1 tgccattcca gtaagtactc aaaagcttta cttatcacca gttttagatg gttttaacag
       61 cgaaattatc gcctataatc tttcaacttc acccaactta gaacaagtac aaacaatgtt
      121 ggaacaggca ttcacagaga agcactacga gaatacgatt ctccatagtg accaaggctg
      181 gcaatatcaa cacgattctt atcatcagtt tcttgagggt aagggaattc aagcatccat
      241 gtcacgtaag ggtaacagcc cagacaatgg tatgatggag tccttctttg ggattctgaa
      301 atcggaaatg ttttacggtt atgagaagtc gtttcagtcg cttaagcaat tggaacaagc
      361 cattgtagac tatattgatt actacaacaa taaacgaatt aaggtaaaac taaaaggact
      421 tagccctgtg caatacagaa ctaaatcctt cggataaatt aattgtctaa ctttttgggg
      481 tcagtacaga actggcgttt tattttttag gatcttgatg ggttaggttc aatccccaag
      541 ggaccaaatt ttcagtttta tttttgatac gagctatatt gtccttatga cgaatgataa
      601 tcaaactagc aagtgctaag ataatagcga tgaagagaga gtcatagtta ctcaggataa
      661 aaccaaaaag tggaaagagc agaaccccga taacagccgc gatcgatgct gtgacactag
      721 acagtgaaat catactgcca agatagagag ctccaaagaa gataatcgca aggtagagac
      781 agaagatagg cgcaaatccg aaaatcactc cagcactggt tgcgacagcc ttaccacctt
      841 taaatcctgc aaatataggg aaggtatggc cgataacagc caaaagtcca aagatgagag
      901 gagaaacgcc ttgtagatga aaaataatcg gaagcagcgt tgctagggtt cctttgaaaa
      961 agtcaatcac aaaggttgcc ataccagctt tcttacctaa aatgcggaag gtgttggtcg
     1021 ttccagtgtt accagaacca tgctcgcgta gattgatttg aaagaatact tgtccaatcc
     1081 agagaccaga tggaatcgaa cccagcagat aggctaggat taataaaact attgtaatca
     1141 tactcctatt atatcatgaa ttgggaaaga aaagcagaga atctctgctg aaattgtcac
     1201 atcggagaaa gaaaaatttt gcaaaatcct tggaaaacct gtagaatagt aaagatgaac
     1261 gaataggagg ttccttgtgt caaaaaagga aatcaatatt aacaattata atgatgatgc
     1321 tattcaggtg ctagaagggt tggatgcggt ccgaaaacgt ccagggatgt atattggatc
     1381 gaccgatggc gctggtcttc atcacctagt ttgggaaatc gttgataatg cagtcgatga
     1441 agccttgtct gggtttggtg atcgtattga tgtaactatc aataaagacg gtagtctaac
     1501 ggttcaagac catggacgtg ggatgccgac aggtatgcac gctatgggaa ttccaactgt
     1561 tgaggttatc tttaccattc ttcatgccgg agggaaattc ggtcaaggtg gctataagac
     1621 atcaggtgga cttcacggag tgggttcttc cgttgttaac gccctttcta gctggttaga
     1681 agttgaaatt acccgtgatg gcgcagttta caagcaacgt tttgaaaatg gtggaaaacc
     1741 tgtcacgact ttgaagaaaa tcggtacagc acccaagtct aaaacaggca ccaaagttac
     1801 ttttatgcct gacgcgacta tcttttctac gacagatttc aagtacaata ccatttcaga
     1861 gcgccttaat gaatcagcct ttctcttgaa aaatgtgacc ttgtctttaa cggacaagcg
     1921 aacagatgaa gcgattgagt tccactatga gaatggagta caagattttg tttcttatct
     1981 caacgaagat aaggaaatct tgacgccagt tctttacttt gaaggggaag acaatggttt
     2041 tcaagtggaa gtagccctcc agtacaatga cggattctca gataacattc tatcctttgt
     2101 caataacgtt cgcaccaagg acggtggaac gcacgagaca ggactcaagt ctgccattac
     2161 caaggtcatg aatgactatg cacgtaaaac aggtcttctc aaggaaaaag ataaaaacct
     2221 tgaaggttca gactatcgtg agggactagc ggccgttctt tctatcttag ttcctgaaga
     2281 acacttgcag tttgaaggac agaccaagga taaactagga agccccctag ctcgcccagt
     2341 tgtggatgga atagtggctg ataagttgac ctttttcctt atggaaaatg gggaattagc
     2401 ttctaacctc atccgcaagg ctatcaaggc ccgtgatgct cgtgaagcag cacgtaaggc
     2461 gcgtgatgag agccgaaatg ggaagaaaaa caagaaagat aagggcttgt tgtctgggaa
     2521 attgacccca gcccaatcta agaatcctgc taagaatgaa ctctatctag ttgaggggga
     2581 ctctgccggt ggttctgcca aacaaggtcg tgaccgcaag ttccaggcta ttctacctct
     2641 tcgtggtaag gttgtcaata cagccaaggc caagatggcg gatatcctca aaaatgaaga
     2701 gatcaatacc atgatttata ccattggtgc gggtgttgga gcagacttct ctattgaaga
     2761 tgccaactat gataagatca ttatcatgac cgatgcggat accgacggtg cccatatcca
     2821 gaccttgctc ttgacatttt tctaccgtta catgcgtccg ctagtcgagg caggtcatgt
     2881 ctatattgcc ctcccacctc tttacaagat gtccaaaggt aaaggcaaga aagaagaagt
     2941 ggcctacgct tggacggacg gagaactaga agaactccgt aaacagttcg gtaaaggcgc
     3001 taccctccaa cgatacaaag gacttggtga gatgaatgcg gaccagctct gggaaacaac
     3061 catgaaccca gaaacacgta ccctcatccg tgtcacaatt gaagatttag cgcgcgccga
     3121 acgccgcgtc aatgttctca tgggagataa ggtagaacca cgccgtaaat ggattgaaga
     3181 taatgtcaag tttacgctag aagaagcgac agtgttttaa tgaaagaaaa taatatgtta
     3241 gtaaactgga aattagagtc ggatgtgaat gactatgtga aaaagcagtt tgaaaatctt
     3301 ggtttgaaaa aattgcagga ttattaatga agaatctgct atgagtgctt atctcaaaga
     3361 ggcgtttaag ggagcggcta agacacaaac taagactaat tttggaaaac catattttca
     3421 tattgaaaaa tacaaacagg atagtttggt tacttatcgt aactgaacac gggacgaatt
     3481 cccgtaaaat atcttttact gcataaaaac ctactctaca ttctttgaaa ggagttgaac
     3541 acgccctaga tactgtgtga aaaagataaa ttctcttgtg agtttgctta cttcaagaat
     3601 tttctatttt cacttggtat tttatgggct ttgtatctta tgtctaacat tcaaaacatg
     3661 tccctggagg acatcatggg agagcgcttt ggtcgctact ccaagtacat tattcaagac
     3721 cgggctttgc cagatattcg tgatgggttg aagccggttc agcgccgtat tctttattct
     3781 atgaataagg atagcaatac ttttgacaag agctaccgta agtcggccaa gtcagtcggg
     3841 aacatcatgg ggaatttcca cccacacggg gattcttcta tctatgacgc catggttcgt
     3901 atgtcacaga actggaaaaa tcgtgagatt ctagttgaaa tgcacggtaa taacggttct
     3961 atggacggag atcctcctgc ggctatgcgt tatactgagg cacgtttgtc tgaaattgca
     4021 ggctaccttc ttcaggatat cgagaaaaag acagttcctt ttgcatggaa ctttgacgat
     4081 acggagaaag aaccaacggt cttgccagca gcctttccaa acctcttggt caatggttcg
     4141 actgggattt cggctggtta tgccacagac attcctcccc ataatttagc tgaggtcata
     4201 gatgctgcag tttacatgat tgaccaccca actgcaaaga ttgataaact catggaattc
     4261 ttgcctggac cagacttccc tacaggggct attattcagg gtcgtgatga aatcaagaaa
     4321 gcttatgaga ctgggaaagg gcgcgtggtt gttcgttcca agactgaaat tgaaaagcta
     4381 aaaggtggta aggaacaaat cgttattact gagattcctt atgaaatcaa taaggccaat
     4441 ctagtcaaga aaatcgatga tgttcgtgtt aataacaagg tagctgggat tgctgaggtt
     4501 cgtgatgagt ctgaccgtga tggtcttcgt atcgctatcg aacttaagaa agacgctaat
     4561 actgagcttg ttctcaacta cttatttaag tacaccgacc tacaaatcaa ctacaacttt
     4621 aatatggtgg cgattgacaa tttcacacct cgtcaggttg ggattgttcc aatcctgtct
     4681 agctacatcg ctcaccgtcg agaagtgatt ttggcgcgtt cacgctttga caaagaaaag
     4741 gctgagaaac gtctccatat cgtcgaaggt ttgattcgtg tgatttcgat tttggatgaa
     4801 gtcattgctc ttatccgtgc ttctgagaat aaggcggacg ccaaggaaaa cctcaaagtt
     4861 agctatgatt ttacggaaga acaggctgag gctatcgtaa ctttgcaact gtaccgtttg
     4921 accaataccg atgtggttgt cttgcaggaa gaagaagcag agcttcgtga gaagattgct
     4981 atgctggtgg ctattatcgg tgatgaaagg actatgtaca atctcatgaa gaaagaactt
     5041 cgtgaggtca agaagaaatt tgcaactcct cgtttgagtt ctttagaaga cactgcgaaa
     5101 gcaattgaga ttgatacagc tagtcttatc gctgaggaag atacctacgt cagcgtgacc
     5161 aaggcaggtt acatcaagcg taccagtcca cgttcctttg cggcttccac cttggaagaa
     5221 attggcaagc gtgatgatga ccgtttgatt tttgttcaat ctgccaagac aacccagcac
     5281 ctcttgatgt tcacaagtct tggaaatgtc atctacagac caatccatga gttggcagat
     5341 attcgttgga aggacatcgg agagcatctg agccaaacca tcacaaactt tgaaacgaat
     5401 gaagcaatcc tttatgtgga agtactggat cagtttgacg atgcgacaac ctactttgca
     5461 gcgactcgcc ttggtcaaat caaacgggta gagcgaaaag aattcactcc atggcggacc
     5521 tatagatcta agtctgtcaa gtatgctaag ctcaaagacg atacagatca gattgtagca
     5581 gtggctccga ttaaactaga tgatgttgtc ttggttagtc aaaatggtta tgccctgcgt
     5641 ttcaatatcg aagaggttcc ggttgtcggt gctaaggcag caggtgtcaa ggctatgaat
     5701 ttgaaagaag atgatgtcct ccaatctggc tttatctgta atacttcgtc cttctacctc
     5761 ttgacccagc gtggaagctt gaaacgtgtt tccattgagg aaattctagc aaccagccgt
     5821 gccaaacgag gattacaagt cttgcgtgag ttgaaaaaca aaccgcatcg tgtcttcttg
     5881 gcaggagcag ttgcagagca aggatttgtt ggcgatttct tcagtacgga agtggatgtg
     5941 aacgaccaaa ctctgcttgt ccaatccaat aaaggaacaa tctatgaaag ccgattgcaa
     6001 gacttgaact tgtcagaacg cactagcaat ggaagcttca tttctgacac gatttcagat
     6061 gaagaagttt ttgacgctta tcttcaggaa gtagttactg aagataaata agtgaaatga
     6121 agaatcagtt ctaagagctg gtttttattg gagaaatttt ctgaaaatta caaaatatac
     6181 ttgctatttt agaaaaatag tgtagaatat aagaaatagg tttttagaat aaggagtgga
     6241 atatgacagt aacgattgat tgggaaaacc tcggtttttc ctatatgaaa ttaccttatc
     6301 gctatcttgc tcatttcaaa aatggacaat gggatcaagg agagcttaca gaggatgcaa
     6361 ctttgcatat ttcagagtct tctccaagtc ttcactatgg acaacaagca tttgaaggtt
     6421 tgaaagctta tcgtactaag gatggcagtg ttcaactgtt ccgtcctgat gaaaatgcta
     6481 aacgcctgca acgtacatgt gaccgtctct tgatgccaca agttccgaca gacatgtttg
     6541 tagaagcttg taaagcagtt gtccgtgcga atgaagaata cgtaccacca tacggaatag
     6601 gtggaacctt atatcttcgc cctcttttga ttggtgtcgg agatattatc ggggtaaaac
     6661 cggcagaaga atacattttc accatctttg ctatgccagt tggaaattac tttaaaggtg
     6721 gtttggtccc aaccaacttc ttgattcagg atgagtacga ccgtgcagca ccaaatggta
     6781 caggtgcggc taaggttggt ggaaactatg ctgcaagtct cttaccagga aaaatggcca
     6841 agtcacgtca tttctcagat gttatctatc tggacccatc aactcataca aagattgaag
     6901 aagtcggatc agctaacttc tttggaatta cagctgataa tgaatttgta acaccattga
     6961 gtccatctat cttgccatct attaccaagt attccttgct ttatttggca gaacatcgct
     7021 tgggattaac tcctattgag ggtgatgttc caattgataa tcttgaccgt tttgtagagg
     7081 caggtgcctg tggtacagca gcggttattt ctccaattgg aggtattcaa catggtgatg
     7141 atttccatgt attctatagt gaaacagaag taggtcctgt gacgcgtaaa ttatataatg
     7201 aattgacggg tattcagttt ggcgatattg aagcgccaga aggttggatt gtaaaagtag
     7261 attaaaataa accaaaggag attttttatg aaatagaaaa agcggctctt aacagcagga
     7321 gtggtcctga gcacgtcagc tattttagtg gcttgtggaa aaactgataa agaagcagat
     7381 gcaccgacaa cattttctta tgtctatgca gtagatccag catcattggg ctacagtata
     7441 gcgactcgaa catcgacgac agacgttatt ggaaatgtta ttgatggttt gatggaaaat
     7501 gataaatacg gcaatgttgc tccttctcaa aaagactatg atttgaatag tacaggatgg
     7561 gctccaagct atcaagatcc agcgtcttac ttgaatatta tggatccaaa atctggttct
     7621 gccatgaaac accttggcat tacgaaagga aaagataagg atgttgtagc taaacctggt
     7681 ttggataaat ataagaaatt gttagaagat gctgtttctg agaccactga cctagagaag
     7741 agatatgaaa aatatgccaa agctcaagct tggtcgacag atagttcatt attgatgcca
     7801 acagcttcat ctggtggttc tccagttgta agtaacgtag taccattctc aaaaccatac
     7861 tcacaagttg gtattaaggg ggaaccatat atctttaaag gaatgaaatt gcaaaaagat
     7921 attgttacaa caaaagaata taacgaggtt tttaaaaaat ggcaaaaaga aaaattggaa
     7981 tccaatagca aataccaaaa agaactagaa aaatacatta aataaggaat ggtattgatc
     8041 ttgataaaat ttttaaaata ctgtcatttt gaatataaag gagtttgata tggagtggat
     8101 tagattaata ggaatagcaa tcattgttgt gggttttatt ttaaaatttg atacaattgc
     8161 aacagtagtc ttagctggtt tggttacagc tttagtttca ggtgtttctc tcgttgaatt
     8221 tttggagatt ttgggaaaag aatttagcaa tcagcgagtg ctcacgattt ttatggttac
     8281 cttgcctctt gtggggctgt cagaaacctt tggactcaag caacgatcaa tcgatttgat
     8341 tcgaaagatt aaaggtctga cagttggaaa cttctataca gtttatttct ttattcgaga
     8401 gttagctggt ttcttttcaa ttcgtctagg aggacaccct cagtttgtca gacctttggt
     8461 tcaacctatg ggagaagcag ctgcagagtc tcaattaggt agaaagttaa cagaggttga
     8521 agatgagaca ataaaagcgc gtgcggctgc gaatgaaaat tttggaaatt tctttgctca
     8581 aaatacgttt gtaggtgctg ggggagtcct cttgataggg ggaacattag atcagttagg
     8641 ctatgaaagc aattatgcag ggattgcttc tacatctatt attgttgctg ttataacact
     8701 tattgtagtg gggatttaca attatttatt tgataaaaaa ttgatatcaa aaaagactag
     8761 gggaggagaa caaaaatgac agagttggca aagcaactat tagagttgac ctatattgtg
     8821 attggttgtc aatttctcca tacagcctat tgtagttata aagataaaac aaacccagtt
     8881 cgacttggga catctgcatt ttggactcta ttgtctatta cgtttatagg tggttcctat
     8941 atgccaaata tgagtattgg tattattgta atcctattat cgctgttaac attgtttaag
     9001 caagtccgta tcggaacctt gccatcctta gatgaaatga aagccaatat tgaatctaac
     9061 aggttgaaaa ataaaatttt tattccagtt atgctgatgg caatacttgc gttggtctta
     9121 gcgcaaatga ttccagaatt tagcaagatt tcgattagcc ttgccgcctt gtttgctaca
     9181 atttctgttc ttgtgattac caatagtcac cctaagagtc tgttatcaga aaataatcga
     9241 atgactcagc aagtttcaac aagtgggatt gttcctcaat tattaggggc tttgggggct
     9301 atttttactg tagcaggtgt tggtgatgtt atctctcatc tgattagcgg tattgttcct
     9361 tcagatagtc gctttatagg agttttggct tatgttcttg gaatggttct attcacaatg
     9421 attatgggaa atgcttttgc agcattcacc gttattacag caggtgttgg agttcccttt
     9481 gtatttgctc tgggagctaa tccaattgtg gctggtgctc ttgccatgac agcaggttat
     9541 tgtgggacct tattgacccc aatggctgct aattttaacg ctctaccagc agcattgatg
     9601 gatatgaaag atcagaatgg cgttataaag gctcaagcag gtgttgctct agtaatgatt
     9661 gttattcaca tattcttaat gtactttctc gcattttagt aaaggaaaat aaaatgaaaa
     9721 tattggttac aggttttaat ccttttggag gtgaaaagat taatccagct ttggaggctg
     9781 taaaattatt accatctgag attaatgggg ctgaagttcg ctgggtagaa attccaacgg
     9841 ttttttataa gtcgtcagaa gttttagagg cagaaatatt acgatatcaa ccagatgctg
     9901 tactttgtat tggacaagca ggcggcagga ccggcttaac acctgaacga gtggctatta
     9961 atcaagatga tgctcgcata cctgataacg aaggcaatca accaattgat acaccgattc
    10021 gtattgatgg agcatcggcc tattttagta gtttacctat caaagcgatg gtacaagcta
    10081 tcaaaaaaca gggacttccg gcagttgtat ccaatagtgc aggaaccttt gtttgcaatc
    10141 atttgatgta ccaagctctt tatttagtgg ataaaaaatt ccctaatatg agagcaggtt
    10201 tcatgcatat tccatatatg atggaacaag tagtaaataa gccgaatact gcaggaatga
    10261 gtctatgtga tattgtaaga ggcatagagg tagctattga agctattgta gactacaaag
    10321 ataaggattt gcagttagta ggcggtgaga ctcactgata acctaaaaag gatagtcaat
    10381 tatgcttgtt tactaactat taactatgct aaatcaattg aggttgttta cataaaactc
    10441 tatatcagag aagcctgata tagagttttt tcttgctagt tttaggattt tcttgtaaaa
    10501 tagaaaaagt gaagagaggt atgaaatgag caagaaagat aaaaaaatcg aaattcaagt
    10561 agcggatgcc aaagttaatg ttggtaaaga cagttttgaa ggttatacat tgactatcgg
    10621 taaaaaagtt atcggagaaa ttgccgaatt agacggacaa tttgccatta taaagaatgg
    10681 gaatgtcgat agtttttata aaaaattgga aaaagctgtg gaaattttga ttgaaaatta
    10741 taatttagca aaataagtct tgtttttttg aaattttcat gatataatag tccatgttga
    10801 ttgtaggaga gatagcgaag aggctaaacg cggcggactg taaatccgct ccttcgggtt
    10861 cgggggttcg aatccctctc tctccatttc attaatgggg tatagccaag cggtaaggca
    10921 agggactttg actccctcat gcgttggttc gaatccagct accccagttc ttaggtaata
    10981 atcaagatag aaagcaaaat atcttagggt attttatttt tataattgaa agacgtgaat
    11041 gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc aagtttaaga
    11101 aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc taaatagcgt
    11161 tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg atgcgactca
    11221 agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc gcgaattgac
    11281 aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat tggatgttct
    11341 tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg tatctaaaaa
    11401 acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag aagttgttac
    11461 tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag gtgttcgtgg
    11521 atttatccca gcttcaatgt tggatactcg tttcgtacgt aacgctgagc gttttgtagg
    11581 tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct tcatcctttc
    11641 acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat tcggtaaatt
    11701 ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg gcgctttcat
    11761 cgaccttggt ggtgttgacg gattggttca cttgactgaa ttgtcacatg aacgtaacgt
    11821 atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc ttgatcttaa
    11881 cgaagaagaa ggacgtgtat cactttcact taaagcaaca gtaccaggac catgggatgg
    11941 cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac gtttgactga
    12001 cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg tatcacaaat
    12061 ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag aagttcaagt
    12121 taaagttctt gaagttaacg cagatgcaga acgcgtgtca ctttctatta aagctcttga
    12181 agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc gtccacgtcg
    12241 tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag gattttcaat
    12301 ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa aatcctttgt
    12361 ttactaaaca agggattttt ctggctcttt gtcaactgta gtgggttgaa gtcagctaag
    12421 cttgagaaag gacaaatttc gtcctttctt ttttgatgtt caaagcgata aaaatccgtt
    12481 ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg cttgataagt ttgatgagat
    12541 tattggtcgc ttccagtttg gcattagaat agtgtagttg aagggcgttg ataatctttt
    12601 ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat aggatgagac tgcttaagat
    12661 tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctggaagtga aacagcaaga
    12721 gctgatagag ctgatagtgg tgtttcaagt cttgtgaata gctcaaaagc ttgtctaaaa
    12781 tctctttatt ggttaaatgc atacgaaaag taggacgata aaatcgctta tcactcagtt
    12841 tacggctatc ctgttgtatg agcttccagt agcgcttgat agccttgtat tcatgggatt
    12901 ttcgatgaaa ctgattcatg ttttgaacac gcacacgact catagcacgg ctaagatgtt
    12961 gcacaatgtg aaagcgatca agaacgattt tagcattcgg gagtgaaaca gtctgggaga
    13021 ctgtttcagc ctgagcctag aaatttgaaa gcgaagctgt ttagccaagt catagtaagg
    13081 gctaaacata tccatagtaa taattttgac gcgacatcgg acagctctat cgtagcgaag
    13141 aaagtgattt cgaatgatag cttgtgttct tccctcaaga acagtgatga tattgagatt
    13201 gttaaaatct tgcgcaatga agctcatctc catctcccga ttgaaacagt cactccccgg
    13261 actgtttcaa cgtcccagga cataatctca ggaagacgcg aaaaatcatg ctcaaagtga
    13321 gaatcattga gcttgcgaat gacagttgaa gttgagatgg aaagctgatg ggcaatatca
    13381 gtcatagaaa tcttttcaat taacttttga gcaatttttt ggttgatgat acgagggatt
    13441 tggtgatttt tctttaccag gggagtctca gcaaccatca tttttgaaca gtgatagcac
    13501 ttgaaacggc gttttctaag gagaattcta gaaggcatac cagtcgtttc aagataagga
    13561 attttagaag gtttttgaaa gtcatatttc ttcatttgac ttccgcactc aggacaagat
    13621 ggagcttcat agtccagttt ggcgatgatt tccttgtgtg tatccttatt gatgatgtct
    13681 aaaatctgga tattagggtc tttaatgtct agtaattttg tgataaaatg taattgttcc
    13741 atatgaatct ttctaatgag ttgtttgatc gcttttcatt ataggtcata tgggactttt
    13801 tttctacaca aaaataggct ccataatatc cataggggat ttacccacta caaatattat
    13861 agagcccaaa atcctttgtt tactaaacaa gggatttttc ttttgtctct gctccttttt
    13921 tgatataata gttctatgtt aaaatcagaa aaacaatcac gttatcaaat gttaaatgaa
    13981 gaattgtcct tcctattgga aggcgaaacc aatgttttgg ctaatctttc caacgccagt
    14041 gctctcataa aatcacgttt tcctaatacc gtatttgcag gcttttattt gttcgatgga
    14101 aaggaattgg ttttaggccc cttccaagga ggtgtttcct gcatccgtat tgcactaggc
    14161 aagggtgttt gtggtgaggc agctcacttt caggaaactg ttattgttgg agatgtgacg
    14221 acctatctca actatatttc ttgtgatagt ctagctaaaa gtgaaattgt ggtgccgatg
    14281 atgaagaatg gtcagttact tggagttctg gatctggatt cttcagagat tgaggattac
    14341 gatgctatgg atcgagatta tttggaacaa tttgtcgcta ttttgcttga aaagacagca
    14401 tgggacttta cgatgtttga ggaaaaatct taatgtatca agcactttat cgaaaatata
    14461 gaagtcaaaa cttctcccag ttagttggtc aagaagttgt ggctaagact cttaaacaag
    14521 cggtggagca agagaaaata agtcacgctt atcttttttc tggtcctcgt ggaacgggaa
    14581 aaaccagtgt tgctaaaatc tttgccaagg ctatgaactg tcccaatcaa gtgggtggcg
    14641 aaccttgcaa taactgctat atttgtcaag cagtgacgga cggtagttta gaagatgtca
    14701 ttgaaatgga tgcagcttct aataatgggg tagatgaaat tcgcgaaatt cgtgataaat
    14761 ctacctatgc gcctagcctt gctcgttata aggtttatat catagatgag gttcacatgc
    14821 tgtctacagg ggcttttaat gccctcctaa agacgctgga agaaccaaca cagaatgtag
    14881 tctttatttt ggccactact gaattgcaca agattcctgc tactattcta tcccgtgtgc
    14941 aacgttttga gtttaaatca attaagacac aggatattaa ggaacatatt cactatatct
    15001 tagaaaaaga aaatatcagt tctgaaccag aggctgtgga aatcattgcc agacgggcgg
    15061 aaggtggaat gcgggacgcc ttgtctattt tggatcaagc cctgagtttg acacagggaa
    15121 atgagctgac gactgctatc tctgaagaaa ttactggcac cattagccta tcagccttgg
    15181 atgattatgt ggcggccttg tctcaacagg atgttcccaa agctttgtct tgcttgaatc
    15241 ttctttttga caatggtaag agcatgactc gttttgtgac cgatcttttg cactatttaa
    15301 gagacttgtt aattgttcaa acagggggag aaaatactca tcatagttca gtctttgtag
    15361 aaaatttggc acttcctcaa aaaaatctgt ttgaaatgat tcgcttagca acagtgaatt
    15421 tagcagatat taagtctagt ttgcagccca agatttatgc tgaaatgatg accgtccgtt
    15481 tggcggaaat caagcccgaa ccagctctat caggagcggt tgaaaatgaa attgctacgc
    15541 tgagacagga agttgcccgt ctcaaacaag agctttctaa tgcaggtgcg gttcctaaac
    15601 aagttgcacc agctcctagt cgaccagcta cgggcaaaac agtctatcgt gtcgatcgca
    15661 ataaagtgca atctatctta caagaggccg tcgaaaatcc tgatttagca cgtcaaaatc
    15721 taattcgttt gcagaatgcc tggggagagg taattgaaag tctaggtggg ccggacaagg
    15781 ctctgctagt tggttctcaa ccggttgctg ccaatgaaca ccatgctatt cttgcttttg
    15841 agtctaactt caatgctggt caaactatga aacgagacaa tctcaatacc atgtttggta
    15901 atatcctcag tcaggcggca ggtttttcac ctgagatttt agctatttcc atggaggaat
    15961 ggaaagaagt tcgcgcagcc ttttcagcca aagccaaatc ttctcaaact gaaaaagaag
    16021 tagaagaaag cctgattcca gaaggatttg aatttttggc tgataaagtg aaggtagagg
    16081 aagactaaag aaagatttca tgatacaata agtttatgaa taaacaacaa tttattatta
    16141 tggcgctatt tacagctgct gagacctatt ttttcaatga agcctggatg actggccgct
    16201 atattatggc agccttttgg gcaattttac tctttagaaa tttccgagtc agttatgtga
    16261 tgggtaaaat tgttgatgtc atcgatcagc attttaatag gaaagactag ccctcagctt
    16321 ccagacaaaa tcaaagcctt ttaggctttt ttttgttata ctagaaaagt atatttatag
    16381 aatttttgct ctatttctgg ggaaatcaga cgtttttcta gtaagtactg taaaagtttt
    16441 gaaaaagaaa ggaactatca tgtcagtaat agagatcaaa gatcttcacg ttgagattga
    16501 aggaaaagaa attttaaaag gggttaacct gaccctgaaa acaggagaaa ttgccgctat
    16561 catgggacca aatggtacag gtaaatcgac tctttctgcc gctatcatgg gaaatccaaa
    16621 ctatgaagta actaaaggtg aagttttgtt tgatggcgta aacatccttg agttggaagt
    16681 ggatgagcgt gcgcgtatgg gacttttcct tgctatgcaa tacccatcag aaatccctgg
    16741 aattaccaat gctgagtttc ttcgtgccgc tatgaatgcg ggtaaagaag atgatgagaa
    16801 gatttcagtt cgtgagttta ttactaagct agatgaaaaa atggaattgc tcaacatgaa
    16861 agaagaaatg gcagagcgtt acctcaacga aggcttctct ggtggtgaga aaaaacgcaa
    16921 tgaaattctt caacttttga tgttggagcc aacatttgct cttttggacg agattgactc
    16981 aggtcttgat attgacgctc ttaaagttgt gtctaaaggt gtcaatgcca tgcgtggtga
    17041 aggttttggt gctatgatca tcactcacta ccaacgtctt ttgaactata tcacacctga
    17101 tgtggtacac gtgatgatgg aaggtcgtgt tgtcctttct ggtggtccag aattggctgc
    17161 gcgtttggaa cgtgaaggat acgcaaaact agctgaagaa cttggctacg actacaagga
    17221 agaattgtaa ttccctcgta tcttttagga gaagtaaatg actagagaaa atattaaact
    17281 tttttcagaa atgcacgctg aaccaagctg gttggctgat ctccgtcaaa aagcttttga
    17341 caagattgag actttggaat taccagttat tgagcgtgtc aaattccacc gttggaatct
    17401 gggtgatgga acgattacag aaaatgagcc atcagcaaat gttccagatt tcacagcttt
    17461 agatcatcac ttgaagttgg tgcaagtagg aactcaaact gttttcgaac aaactccagt
    17521 tgagttagct gaacagggtg ttgtcttcac agactttcac tcagctttag aagaaattcc
    17581 agagctgatc gaagaattct tcatgtcatc tgttaagtat gatgatgaca agttggcggc
    17641 ttaccacaca gcttacttta acagtggtgc tgtactctat attccagata acgtagaaat
    17701 cacagagcca attgaaggaa ttttctacca agatagcgat agcaatgtgc cgtttaacaa
    17761 gcatattatg attatcgctg gtaaaaattc taagattagt tatctggagc gtttagagtc
    17821 acgcggtgaa ggaagtgaca aagtaactgc caatatcaca gtggaagtga ttgcacgttc
    17881 tggtgcgcaa gtcaagtttg ctgctatcga ccgtctaggt gaaaacgtca ctgcctacat
    17941 tagccgtcgt ggtaaattag gcaacgatgc aagtattgac tgggctatcg gtgtcatgaa
    18001 cgaaggaaat gtcgttgctg attttgatag tgacttgatt ggtaatggta gccatgctga
    18061 cctcaaggtt gtagctcttt caagtggtcg tcaggtacaa gggattgata ctcgtgtaac
    18121 taactatggc tgcaactcta tcggaaatat cctacaacat ggggttatcc ttgaaaaagc
    18181 aactttgact ttcaatggta tcggccacat catcaaggat gctaagggag cagatgcgca
    18241 acaagagagc cgtgttctca tgctttcaga ccaagcgcgt tcagatgcta acccaattct
    18301 tttgattgat gaaaatgatg tcactgcagg acacgcagcg tctatcggac aagttgatcc
    18361 agaagacatg tattacctca tgagtcgtgg attggataag gcaactgcag agcgtttggt
    18421 tgttcgtggt ttccttggat ctgttatcgt ggagattcca gtcaaggaag ttcgtgatga
    18481 aatgattgca actatcgaag agaaattgtc aaaacgctaa ggggaagcct atgttagatg
    18541 tagaagcgat tcgcaaggat tttccaattt tagatcagat tgtcaatgat gaacctctgg
    18601 tctatctgga caatgctgcg acgacacaaa aaccactagt agttctgaaa gctattaaca
    18661 gttactatga gcaggacaat gccaatgttc accgtggtgt ccatacctta gcggaacgag
    18721 cgacagcttc ttatgaagct gctcgtgaaa ccattcgtaa gtttattaat gcaggctcta
    18781 caaaggaagt tctctttacc agaggaacga caaccagcct taactgggtg gcacgctttg
    18841 ctgaggaaat tctcgctgag ggagaccggg tcttgatttc agtaatggaa caccattcta
    18901 atatcattcc atggcaggaa gcttgtcgaa agactggagc agagcttgtc tatgtctatc
    18961 ttaaagacgg tgccttggat atggaggatt tgcgagctaa attgactgat aaggttaaat
    19021 ttgtttccct agctcatgcc tccaatgttc ttggtgtggt caatccgatc aaggaaatca
    19081 ctcaattagc ccaccaagtt ggggcaatta tggtagtgga tggtgctcaa tctacacctc
    19141 atatgaagat tgatgtccag gacttggatc tggacttttt cgccttttcg ggtcacaaga
    19201 tggctggtcc gactggtatc ggtgtccttt acggcaaaga aaagtatctt gagcaaatgt
    19261 ctccagtaga atttggcggc gagatgattg attttgtcta cgagcaattt gctagttgga
    19321 aggaattgcc ttggaaattt gaggctggaa cgccaaatat ggcaggagct attggacttg
    19381 cgactgcagt tgattatctg gaaaagattg gtatggatgc cgttgaagct catgaacagg
    19441 aattgattgc gtacgtctat ccaaaactgc aggcaattga gggattgacc atttacggtt
    19501 ctcaggattt ggctcaacgt tcgggtgtta ttgcctttaa cctaggtgat ctccatcctc
    19561 acgatcttgc gacggctctg gattatgaag gagtggctgt tcgtgctggt caccattgtg
    19621 cgcaaccctt gcttcagtat ttggaagtcc cagcaacagc tcgtgcaagt ttttatatct
    19681 acaataccaa ggcagattgc gacaaactag tcgatgccct acaaaagaca aaggagtttt
    19741 tcaatggcac tttctaaact agatagcctt tatatggcag tggtagcaga ccattcgaaa
    19801 aatccacatc accaagggaa gttagaagat gctgagcaaa ccagtctcaa caatccgact
    19861 tgtggggatg tcatcaacct ctctgtcaag tttgatgcag aggaccgttt ggaagatatt
    19921 gcttttctaa attcaggatg cacgatttca actgcttctg ctagtatgat gacagatgcc
    19981 gttttaggaa aaaccaaaca agaaatttta gaactggcga ctattttttc tgaaatggtt
    20041 caagggcaaa aagatgagcg tcaaggccaa cttggagacg cggcattctt gtcaggtgtt
    20101 gccaaattcc ctcaaagaat caagtgtgca accctagctt ggaatgccct taagaaaaca
    20161 attgaaaatc aagaaaaaca gtaagacaag tttcttttgt cttatgaatt attagaaatg
    20221 aagaaagaaa ggatactatg gctgaagaaa gagtagaacc aaaaccaatt gaccttggtg
    20281 aatataaatt tggtttccat gacgatgtag agcctgtctt atcgacagga aaaggactca
    20341 acgaaggtgt tattcgtgaa ttatctgctg ctaagggtga gcctgagtgg atgttggagt
    20401 tccgtttgaa gtcttatgaa accttcaaaa aaatgcccat gcaaacttgg ggagcagact
    20461 tgtcagagat tgactttgat gacttaatct actaccaaaa accatctgac aaaccagccc
    20521 gttcttggga tgatgtacct gaaaagatta aagaaacctt tgaacgtatc gggattccag
    20581 aagctgaacg tgcttattta gcaggggctt ctgcccagta cgagtcagaa gtggtttacc
    20641 acaacatgaa ggaagagttc caaaaattag gtattatctt tacagataca gattccgcac
    20701 tcaaggaata cccagactta tttaaacaat actttgcgaa gttggtaccg ccgacagata
    20761 acaagttggc agccctcaac tcagcagtat ggtcgggtgg aacttttatc tacgtgccaa
    20821 aaggtgtcaa ggtagatatt ccacttcaaa cttatttccg tatcaataac gaaaatatag
    20881 gtcagttcga acgtaccttg attatcgttg atgagggagc aagcgtccac tacgtagaag
    20941 gatgtacagc accaacatat tcaagcaata gcttacacgc tgccattgta gaaatttttg
    21001 ctttggacgg agcttatatg cgttatacaa ctatccaaaa ctggtctgat aacgtctata
    21061 acttggtaac aaagcgtgct aaggctcaaa aggatgccac tgttgagtgg attgatggaa
    21121 acttgggtgc caaaacgact atgaaatatc catctgttta ccttgatgga gaaggagcgc
    21181 gtggtaccat gctctctatc gcctttgcta atgcagggca acaccaagac acgggtgcta
    21241 agatgattca caatgctcca cataccagct cgtctattgt gtctaaatcc atcgctaaag
    21301 gtggaggaaa ggttgactac cgtggacaag tcacctttaa caagaactct aagaaatctg
    21361 tttcccacat tgaatgtgat accattatca tggatgactt gtcagcatca gatactattc
    21421 catttaatga aattcacaac tcgcaagtgg ctttggaaca cgaagccaaa gtatctaaga
    21481 tttcagaaga gcaattgtat tatctcatga gccgtggatt gtcagaatct gaggcaactg
    21541 aaatgattgt catgggattt gtagaaccct ttacaaaaga acttccaatg gaatacgcag
    21601 ttgagctgaa ccgcttgatt agctatgaaa tggagggatc agttggataa aatttgattt
    21661 tatactcttc gaaaatctct tcaagccacg tcagcatcgc cttaccgtat gtatggttac
    21721 tgacttcgtc agtttcatct acaacctcaa aacagtgttt tgagcaacct gcggctagct
    21781 tcctagtttg ttctttgatt ttgagtatta gatttactca aaatcaagga ttttgaagat
    21841 gaacttgtat caaaaaatcg cggtttaaaa tcgcgatttt ttataatttc tcgttaacaa
    21901 agcggacaaa ctgattccac caaactttta agaagaaggc tttttcaatt ttcttgtctg
    21961 ctaccatttc gaaactaggg cgctctgtgg tgatgtaacc ttgaccaatc aagtccttgt
    22021 cttcataagt caaatggcca accactgttc cagcttcaag tggtgctggg attgctttgg
    22081 aatcaggtgt gaattgaaca gattgggaag attgattccc aatctgttcg attagataga
    22141 tatcctctgg agccactgca gttactgtat cttcttttcc atcttgtaca ggggctttgc
    22201 tatcttgata ggcatcgcct tgttgaacga ttttgcgaag tgtaaatgta gaagaaatat
    22261 aatccattag ggaagatgta gctgtaaatc gagcgtaagg attattgtct tgatgatctg
    22321 catttaaaac aactgtgatg actctcatgc ctttttcgac agtagtacca acaaaagact
    22381 ctccagcctt atctgttgtt cctgttttta gcccatcaaa accaccacgg taagcaggca
    22441 taccttctaa catgtagttg gttgaagtga ttgtcatccc agcaaaagta gaagaaggtt
    22501 ttttggtgat ttctaagact tgtgggtatt ttttgatgag gttgcgagca acgatagcga
    22561 catcataagc actaagctta ttttcctcat cttttttaga acctgggtaa atgttatccc
    22621 ctagagtttc attgttaaga cctgtcgtat tgacaacagt ggcatcctga attccccatt
    22681 ccaagagttt tgcccgcatc atatcgacga aatctttttc tgagccagca attttctcag
    22741 ctagggcaat agcggcgctg ttggcactag ataccagagt tgcttcaagc aactcttcga
    22801 cagtataatt acgggcctct ataggaatat tactggcttc agaatttgtc gtcaattgat
    22861 aaggataatc agaaatatct acaggagtgg agagggtaat acttccgttt tccaaagctt
    22921 catagaccag ataaacagta atcaattttg ttatggaagc aatttcgaca ggttgcgttg
    22981 catccttctc atagagaatt ttaccagtat ttgcctcaac agcaatcgca tgtttagcgg
    23041 caatggtaaa atcttgagca acagcagtag aagcaccccc taaaagagag acagttaaca
    23101 aagttaaaaa tatttttttc atagtagtct tattctatca taaagaaaaa aaatattctt
    23161 gctttaataa ttcatctgtt aagctttttg aaaatatggt aaaataaagt gagggaggta
    23221 actcatgttt cgtagaaata aattattttt ttggaccaca gaaattttac tcttaaccat
    23281 catcttttac ctatggagac agatggggtc tttgattaac ccttttgtta gcgtgcttaa
    23341 tacaattatg attccatttt tattaggggt ctttctttat tatttgacaa accctattgt
    23401 tactttctta aataaagtct gtaaactcaa tcgtttgctt ggtattttaa ttaccttgtg
    23461 tactttggtc tggggaatgg tcataggtgt tgtctatctc ttacctattt tgattaatca
    23521 gttatctagt ttgattatat ctagtcaaac tatttatagt cgagtacaag acttaatcat
    23581 agacttatct aattatcctg cgctccagaa tttggatgta gaagctacaa ttcagcagtt
    23641 aaacttatcc tatgttgata ttcttcaaaa tatcctaaat agcgtatcaa atagtgtggg
    23701 gagcgtcttg tcagctctta tcagtactgt tttgattttg attatgactc cagttttttt
    23761 ggtttatttc ttattagatg gacataaatt cttgcccatg cttgaaagaa cgattctaaa
    23821 gagggatcgc ttgcatattg caggcttatt aaagaattta aatgcgacga ttgctcgcta
    23881 tattagtgga gtttcgattg acgcaatcat tataggttgt ttagcttata ttggctatag
    23941 tattattggt ttaaaatatg ctttagtttt tgccattttt tctggtgtag ccaatttaat
    24001 tccttatgtg gggccaagta ttggtttgat tcctatgatc atcgcaaata tattcactga
    24061 tccccataga ctgctgattg cagtgattta tatgcttgtt gttcagcagg tagatggcaa
    24121 tatcttatat cctcgaatcg taggaagtgt tatgaaggtt catccaatca cgattttagt
    24181 tttacttttg ttgtcaagca atatttatgg tgtagttgga atgattgtcg cagtgccaac
    24241 ctattctatc ttgaaagaaa tttctaagtt cttatcccgt ttgtatgaaa atcataaaat
    24301 aatgaaagaa cgagaaagag aattagctaa gtaaaagtca ggagaaccct gatttttctt
    24361 tactggaagt ggcctttaga ttagaagact gaaaataagt taaagtctta aactaatttt
    24421 cacagctaag aatagtagaa gttaatctga taaaaatcga aaaaaccagt ggaattctgt
    24481 gtcagggtaa gttccactgg ttttcatagt ctattaaagt tcgaatgaaa cctatttata
    24541 gtagattgaa actagaatag tacacctcta attctaaaac attgttagaa atcgatttga
    24601 ctgtcctgat c
//
LOCUS       DBERCA010000022        23649 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.022, whole genome shotgun sequence.
ACCESSION   DBERCA010000022 DBERCA010000000
VERSION     DBERCA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.022"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            184..414
                     /gene="blpU"
                     /locus_tag="ACL719_001072"
                     /pseudo
     CDS             184..414
                     /gene="blpU"
                     /locus_tag="ACL719_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
     gene            417..542
                     /locus_tag="ACL719_001073"
     CDS             417..542
                     /locus_tag="ACL719_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS0699056.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            1129..3282
                     /gene="comA"
                     /locus_tag="ACL719_001074"
     CDS             1129..3282
                     /gene="comA"
                     /locus_tag="ACL719_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGS0699057.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3295..4644
                     /gene="comB"
                     /locus_tag="ACL719_001075"
     CDS             3295..4644
                     /gene="comB"
                     /locus_tag="ACL719_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGS0699058.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKEDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLINQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4814..5521
                     /gene="purC"
                     /locus_tag="ACL719_001076"
     CDS             4814..5521
                     /gene="purC"
                     /locus_tag="ACL719_001076"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS0699059.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5578..9303
                     /locus_tag="ACL719_001077"
     CDS             5578..9303
                     /locus_tag="ACL719_001077"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGS0699060.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERVNGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVAIVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELETALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEVANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9396..10838
                     /gene="purF"
                     /locus_tag="ACL719_001078"
     CDS             9396..10838
                     /gene="purF"
                     /locus_tag="ACL719_001078"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGS0699061.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGKLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEDRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10875..11897
                     /gene="purM"
                     /locus_tag="ACL719_001079"
     CDS             10875..11897
                     /gene="purM"
                     /locus_tag="ACL719_001079"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGS0699062.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11894..12439
                     /gene="purN"
                     /locus_tag="ACL719_001080"
     CDS             11894..12439
                     /gene="purN"
                     /locus_tag="ACL719_001080"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGS0699063.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12523..13032
                     /locus_tag="ACL719_001081"
     CDS             12523..13032
                     /locus_tag="ACL719_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS0699064.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13057..14604
                     /gene="purH"
                     /locus_tag="ACL719_001082"
     CDS             13057..14604
                     /gene="purH"
                     /locus_tag="ACL719_001082"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGS0699065.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDMTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADNIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14726..15988
                     /gene="purD"
                     /locus_tag="ACL719_001083"
     CDS             14726..15988
                     /gene="purD"
                     /locus_tag="ACL719_001083"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGS0699066.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16245..16361
                     /locus_tag="ACL719_001084"
     CDS             16245..16361
                     /locus_tag="ACL719_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS0699067.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            16391..16879
                     /gene="purE"
                     /locus_tag="ACL719_001085"
     CDS             16391..16879
                     /gene="purE"
                     /locus_tag="ACL719_001085"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGS0699068.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16866..17957
                     /gene="purK"
                     /locus_tag="ACL719_001086"
     CDS             16866..17957
                     /gene="purK"
                     /locus_tag="ACL719_001086"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGS0699069.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17967..18194
                     /locus_tag="ACL719_001087"
     CDS             17967..18194
                     /locus_tag="ACL719_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS0699070.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVKAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18257..19555
                     /gene="purB"
                     /locus_tag="ACL719_001088"
     CDS             18257..19555
                     /gene="purB"
                     /locus_tag="ACL719_001088"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGS0699071.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19610..23548)
                     /gene="strH"
                     /locus_tag="ACL719_001089"
     CDS             complement(19610..23548)
                     /gene="strH"
                     /locus_tag="ACL719_001089"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGS0699072.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6453 a         4677 c         5679 g         6840 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttga
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagta
      301 gcacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gcgggaggag ctatacttgg aggtgtggcc tatgaagcga catgttggtg gtaattatgg
      421 attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat atggatgatt
      481 taattagaaa aaaattttta aagtcttcgg agaagaaaac agaaaaatct gttaaaaaat
      541 aatcaaaact ataaatgatg aatctgaatc aaaattattt tgcgcatgta aagaggagtc
      601 ttatagtaac gagtcaaaaa aggagtaact atgaatcgta atttagaacg gtgttatcta
      661 ttctgactag ggatagatca taccataggt agcttagaaa tagcagagac attagaaatt
      721 gaagtaataa ataggatgtc gtaagtgtta ctatcaatga tttatttgtt tcaagcttgc
      781 ctagggtgtc agtaaaaaat cattttcctt tcaatagcat atttttagtg ggcaggactc
      841 ttgttctgcc tattttttta tccaaaaagt gcagttggga gggagatagg ctcatttggg
      901 aaggaagtcc agtttttgtt tagtgattgg ggtaagatag ttgttatcag atgagtttat
      961 attcttcgaa aatcaaattc aaaccacgtc aacgtcgcct tgccgtatat atgtgactga
     1021 cttcgtcagt cctatctaca acctcaaaac agtgttttga gcagcctgcg gctagtttcc
     1081 tagtttgctc tttgattttc attgagtatt agggaaaagg agatgaatat gaaatttggg
     1141 aaacgtcact atcgtccgca agtggatcag atggactgcg gtgtagcttc attagccatg
     1201 gtttttggct actatggtag ttattatttt ttggctcact tgcgagaatt ggctaagacg
     1261 accatggatg gaacgacggc tttgggcttg gtcaaggtgg cagaggagat tggttttgag
     1321 acgcgagcca ttaaggcgga tatgacgctt tttgacttgc cggatttgac ttttcctttt
     1381 gttgcccatg tgcttaagga agggaaattg ctccactact atgtggtgac tgggcaggat
     1441 aaggatagca ttcatattgc cgatccagat cccggggtga agttgaccaa actgccacgt
     1501 gagcgttttg cggaagaatg gacaggagtg actcttttta tggcacctag tccagactac
     1561 aagcctcata aggaacaaaa aaatggtctg ttctctttta tccctatatt agtgaagcag
     1621 cgtggcttga ttgccaatat cgttttggca acactcttgg taaccgtgat taacattgtg
     1681 ggttcttatt atctgcagtc tatcattgat acctatgtgc cagatcagat gcgttcgaca
     1741 ctagggatta tttctattgg gctagtcatc gtctacatcc tccagcaaat cttgtcttac
     1801 gctcaggagt atctcttgct tgttttgggg caacgcttgt cgattgacgt gattttgtcc
     1861 tatatcaagc atgtttttca cctccctatg tccttctttg cgacacgcag gacaggggaa
     1921 atcgtgtctc gttttacaga tgctaacagt atcatcgatg cgctggcttc gaccatcctt
     1981 tcgattttcc tagatgtgtc aacggttgtc attatttccc ttgttctatt ttcacaaaat
     2041 accaatctct ttttcatgac tttattggcg cttcctatct acacagtgat tatctttgcc
     2101 tttatgaagc cgtttgaaaa gatgaatcgg gataccatgg aagccaatgc ggttctgtct
     2161 tcttctatca ttgaggacat caacggtatt gagactatca agtccttgac cagtgaaagt
     2221 cagcgttacc aaaaaattga caaggaattt gtggattatc tgaagaaatc ctttacctat
     2281 agtcgagcag agagtcagca aaaggctctg aaaaaggttg cccatctctt gcttaatgtc
     2341 ggcattctct ggatgggggc tgttctggtc atggatggca agatgagttt ggggcagttg
     2401 attacctata ataccttgct ggtttacttt accaatcctt tggaaaatat catcaatctg
     2461 caaaccaagc ttcagacagc gcaggttgcc aataaccgtc taaatgaagt gtatctagta
     2521 gcttctgagt ttgaggagaa gaaaacagtt gaggatttga gcttgatgaa gggagatatg
     2581 accttcaagc aggttcatta caagtatggc tatggtcgag acgtcttgtc ggatatcaat
     2641 ttaaccgttc cccaagggtc taaggtggct tttgtgggga tttcagggtc aggtaagacg
     2701 actttggcca agatgatggt taatttttac gacccaagtc aaggggagat tagtctgggt
     2761 agtgtcaatc tcaatcagat tgataaaaaa gccctgcgcc agtacataaa ctatctgcct
     2821 caacagccct atgtctttaa cggaacgatt ttggagaatc ttcttttggg agccaaggag
     2881 gggacgacac aggaagatat cttacgggcg gtcgaattgg cagagattcg agaggatatc
     2941 gagcgcatgc cactgaatta tcagacagaa ttgacttcgg atggggcagg gatttcaggt
     3001 ggtcaacgtc agagaatcgc tttggcgcgt gctctcttga cagatgcgcc ggtcttgatt
     3061 ttggatgagg cgactagcag tttggatatt ttgacagaga agcggattgt cgataatctc
     3121 atggctttgg acaagacctt gattttcatt gctcaccgct tgactattgc tgagcggaca
     3181 gagaaggtgg ttgtcttgga tcagggcaag attgtcgaag aaggaaagca tgctgatttg
     3241 cttgcacagg gtggctttta cgcccatttg gtcaatagct agaaagagga gaggatgaaa
     3301 ccagaatttt tagaaagtgc ggagttttat aatcgtcgtt accataattt ttccagtagt
     3361 gtgattgtac ccatggccct tctgctcgtg tttttacttg gctttgcaac tgttgcagaa
     3421 aaggagatga gcttgtccac tagagctact gtcgaaccta gtcgtatcct tgcaaatatc
     3481 cagtcaacta gcaacaatcg tattcttgtc aatcatttgg aagaaaataa gctggttaag
     3541 aaggaggatc ttttggttca ataccaagaa ggggcagagg gtgtccaagc ggagtcctat
     3601 gccagtcagt tggacatgct aaaggatcaa aaaaagcaat tggagtatct gcaaaagagc
     3661 ctgcaagaag gggagaacca ctttccagag gaggataagt ttggctacca agccaccttt
     3721 cgcgactaca tcagtcaagc aggcagtctt agggctagta catcgcaaca aaatgagacc
     3781 atcgcgtccc agaatgcagc agctagccaa acccaagccg aaatcggcaa cctcatcaat
     3841 caaacagagg ctaaaattcg cgattaccag acagctaagt cagctatgga aacaggtgct
     3901 tccttggcca gtcagaatct agcctactct ctttaccagt cctacaagtc tcagggcgag
     3961 gaaaatcccc aaactaaggt tcaggcagtt gcacaggttg aagcacagat ttctcagtta
     4021 gaatctagtc ttgctactta ccgtgtccag tatgcaggtt caggtaccca gcaagcctat
     4081 gcgtcagggt taagcagtca attggaatcc cttaaatccc aacacttggc aaaggttggt
     4141 caggaattga cccttctagc ccagaaaatc ttggaggcag agtcaggtaa gaaggtacag
     4201 ggaaatcttt tagacaaggg gaaaattacg gcaagtgagg atggggtgct tcatcttaat
     4261 cctgagacca gtgattctag catggttgca gaaggtgccc tactagccca actttatcca
     4321 tctttggaaa gagaagggaa agccaaactc acagcctatc taagttcaaa agatgtagca
     4381 agaatcaagg tcggtgattc tgttcgctat actacgactc atgatgccgg gaatcaactt
     4441 tttctagatt ctactattac aagtattgat gcgacagcta ctaagactga gaaagggaat
     4501 ttctttaaaa tcgaggcgga gactaatcta acttcggagc aggctgaaaa acttaggtac
     4561 ggggtggaag gccgtctaca gatgattacg ggcaagaaaa gttacctacg ttattatttg
     4621 gatcaatttt tgaacaaaga gtaatgttcg tgtttttaga gttaaataat ttttaaactg
     4681 tgagaaagat tcttcttgca gttttttctt tacaattttt gaaaaacatc tgctatttat
     4741 tcggttgaat tcttgtgttt tttggttttt tgtggtaaaa tgtgctcaag taatacgaaa
     4801 ggcgaacttt aaaatgtcaa aacaattgat ctattcggga aaagctaaag atatctatac
     4861 aactgaggat gaaaatctta ttatttcaac ttacaaggac caggcgactg ctttcaacgg
     4921 tgtcaagaag gagcagattg caggtaaggg agtcttgaat aatcagattt catcttttat
     4981 ttttgagaaa ttaaatgcgg ctggtgtggc gactcacttt gtggagaaac tttcagacac
     5041 ggaacaactc aataaaaagg ttaagattat tcctttggaa gtcgtgctcc gcaactatac
     5101 tgctggttcc ttttcaaaac gttttggtgt ggatgaggga atcgccttgg agactccgat
     5161 tgtcgaattt tactacaaaa atgatgattt ggatgatcca tttatcaatg atgagcatgt
     5221 gaaattccta cagattgcgg gtgaccagca gattgcctac ttgaaggaag aaacgcgtcg
     5281 tatcaatgaa ctattgaaag tctggtttgc tgagattggg cttaaattga ttgactttaa
     5341 gctagagttc ggttttgaca aggatggcaa gattatcttg gcagacgaat tttcaccaga
     5401 taactgccgc ttgtgggacg ctgatggcaa ccacatggat aaggatgttt tccgtagagg
     5461 attgggagaa ctaaccgacg tttatgagat tgtttgggaa aagttgcagg gtttaaaata
     5521 acaacctcaa ggctgtttgg gaatattgca agagctgaaa taaaggaata agaattgatg
     5581 gataaacgta tttttgttga aaaaaaggct gattttcagg tcaagtcaga gagtttggtt
     5641 agagagctcc agcacaacgt gggactgtca agcttgaaaa gtattcgtat tgtgcaagta
     5701 tatgatgtat ttgacttggc tgaggacttg tttgcacctg cagagaagca cattttctct
     5761 gagcagggaa ccgaccatgt tttagatgaa gtatctgtgc aggcggatct tgctaactat
     5821 gctttctttg ccattgaaag tctgccaggg cagtttgacc agcgtgcagc ttcgtcacag
     5881 gaagccttgc ttttgttggg aagttcgagt gacgtgacag tcaacacagc ccaactttac
     5941 ttggtgaata aagatattga tgcgactgag ttggaagctg tcaaaaacta cctgctcaat
     6001 ccagttgatt ctcgtttcaa ggatatcacg acagggattg ccaagcagga gttttcagag
     6061 tcagacaaga ccattccaaa attgactttc tttgaaagct atacagcaga agactttgct
     6121 cgctacaagg ccgagcaagg gatggccatg gaagtggatg atttgctctt tatccaagac
     6181 tactttaagt caatcgggcg cgtgccaact gagactgaac tcaaggtttt ggacacttac
     6241 tggtctgacc actgccgtca tacgactttt gagacagagt tgaaacacat cgacttttca
     6301 gcttctaaat ttcaaaagca attgcagtca acctatgaca agtatattgc catgcgcgag
     6361 gagttaggtc ggtctgaaaa accacaaacc ttgatggata tggcgactat tttcggtcgt
     6421 tatgagcgtg ttaacggacg attggatgat atggaagtct ctgacgaaat caatgcctgc
     6481 tcagttgaaa ttgaagtgga tgttgatggt gtcaaggaac cttggctcct catgtttaaa
     6541 aatgaaaccc acaaccatcc aacagaaatt gagccatttg gtggagcggc tacctgtatc
     6601 ggtggagcta ttcgtgatcc gttgtcaggc cgttcctatg tttaccaagc catgcgtatt
     6661 tcaggtgctg gtgatattac agcaccgatt tcggaaactc gtgctgggaa attgccacaa
     6721 caagtcattt ctaaaacagc agctcatggt tattcttcat atggtaacca gattgggctt
     6781 gccacaacct acgttcgtga atacttccac ccaggctttg tagctaaacg tatggaactt
     6841 ggtgccgttg ttggtgcggc tcccaagggc aatgttgtcc gtgaaaaacc tgaagcaggt
     6901 gatgtgatca tccttctcgg aggcaaaaca ggtcgtgatg gtgtcggtgg tgcgacgggc
     6961 tcttctaagg ttcaaacagt tgagtctgta gagactgctg gtgctgaggt tcaaaaagga
     7021 aatgccatcg aagaacgcaa gattcagcgc ctcttccgta atggcaatgt cactcgtctg
     7081 atcaagaagt ccaatgactt tggggcaggc ggcgtctgtg tggctatcgg tgaattggca
     7141 gacggtcttg aaatcgacct caacaaggtg cctcttaaat accagggctt gaatggtaca
     7201 gaaattgcca tctctgaatc acaagaacgg atggcggtcg tggttcgtcc tgaagatgtg
     7261 gatgccttcg ttgccgaatg taacaaagaa aatattgatg ctgttgtggt ggcgatagta
     7321 actgaaaaac caaatcttgt catgcactgg aatggtgaga caatcgttga cttggagcgt
     7381 cgtttccttg acaccaatgg tgtgcgcgtg gttgtcgatg ccaaagttgt ggacaaggat
     7441 gtcaaactcc cagaagagcg tcaaacatct gctgaaacac tggaatcaga tacccttacg
     7501 gttctatctg acctcaacca tgcaagtcaa aaaggattac agactatctt tgactgctct
     7561 gttggacgct caacggttaa tcacccactt ggtggtcgtt accaactcac accaactgag
     7621 gcatctgtgc agaaattgcc agttcaacac ggtgtgactc atactgcgtc ggtcattgct
     7681 caaggtttca acccatatgt agctgaatgg tctccatacc acggtgctgc ttatgcggtt
     7741 atcgaagcaa ctgctcgttt ggtggctgct ggtgccaact ggtccaaggc tcgtttctct
     7801 taccaagagt atttcgagcg tatggataaa caagcagagc gtttcggtca gccagtagct
     7861 gctcttctag gttctattga agcacaaatt cagcttggct tgccatctat cggtggtaag
     7921 gactccatgt ctggtacctt tgaagaattg accgttccgc caaccttggt tgcctttggg
     7981 gtgacgacgg cagatagccg taaggtgctc tctccagaat ttaaagctgt tggggaaaat
     8041 atctactaca tcccaggtca agccctctct gcagagattg attttgactt gattaagaaa
     8101 aattttgctc agtttgaagc catccaagct gaccataaag tgacatctgc atcagctgtc
     8161 aaatacggtg gtgtagttga aagtttggct cttgctacct ttggaaacta tattggtgca
     8221 gaggtgacct tgcctgaact tgaaacagct ttgacagctc aattaggcgg ctttgtcttc
     8281 acatctcctg aagaaattgc tggagtagag aaggttggac aaacgaaagc agactttaca
     8341 ctgactgtca acggtgtgaa gctagatgga cacaagcttg acagtgcatt tcaagggaca
     8401 ttggaagaag tttacccaac agaatttacc caagcgaaag aactagaaga agtaccagct
     8461 gtggcatcag atgttgtgat taaagccaaa gaaaaggttg aaaaacctgt ggtttacatc
     8521 ccagtctttc caggaaccaa ctcagaatat gattcagcta aggccttcga aaaagaaggt
     8581 gcagaggtca atttggtgcc attcgtgacc ttgaatgaag aagctattgt caagtcagtt
     8641 gaaactatgg ttgacaatat cgacaagact aatattctct tctttgctgg tggattctcg
     8701 gctgcggatg aaccagatgg ttcagctaag tttatcgtca atatcctgct taatgaaaaa
     8761 gtgcgtgtgg ctattgatag ctttatcgcc cgtggtggtt tgattatcgg tatttgtaat
     8821 ggattccaag ccttagtcaa atcgggtctc ctaccctacg gaaactttga agttgctaac
     8881 agtactagcc caaccctctt ctacaatgat gccaaccaac acgtggccaa gatggtggaa
     8941 actcgcattg ccaataccaa ctcaccatgg ttggctggtg tgcaagtggg cgatatccac
     9001 gctattcctg tttcgcacgg tgaagggaag tttgtcgtga cggctgagga atttgcagag
     9061 ctccgtgaca atggacaaat tttcagccaa tacgttgact ttaacggtaa accaagtatg
     9121 gattctaagt acaatccgaa tggttctgtc catgccatcg aaggaattac cagcaagaat
     9181 ggtcaaatca tcggtaagat gggccactca gaacgttatg aggacggtct tttccaaaat
     9241 atcccaggca ataaagacca gcacctgttc gcgtcagcgg ttaaacattt cactggaaaa
     9301 taagacttac agattttcta atagatagta tcagtaatgt aaaagtcatg taaatctagc
     9361 tcttgatgat tacaaatgaa aattaggtat aaaaaatgac atacgaagta aaatctctta
     9421 atgaagaatg tggtgttttc ggtatttggg gacatccaga tgctgctaag ttgacctatt
     9481 ttggactcca cagtcttcag caccgtggtc aagagggggc aggaatcctc tccaatgacc
     9541 aaggaaaact aaagcgccat cgtgacatgg gacttttatc agaagtcttc agaaatcctg
     9601 ctaatttgga taaattgaca gggactggtg cgattgggca tgtgcgttat gcgactgctg
     9661 gcgaagcttc tgtagataac atccagccct tcctcttccg ttttcacgat atgcagtttg
     9721 gcttggctca taatgggaat ctgaccaatg cagcctctct caagaaagaa ttggaagata
     9781 gaggagcaat tttcagtgcg acttcggatt cggaaatctt ggctcatctc attcgtcgca
     9841 gtcataatcc tagcctgatg ggcaaaatca aggaagcgct cagccttgtc aaaggtggtt
     9901 ttgcctatat cttgctgttt gaggacaagt tgattgcggc tcttgacccc aatggtttcc
     9961 gtccgctttc tatcgggaaa atggctaatg gagcagttgt tgtatcttct gaaacctgtg
    10021 cttttgaggt tattggtgcc gagtggattc gtgatttgaa gccaggtgag attgtgatca
    10081 ttgatgacga gggcattcag tatgacagct atacagatga tacccagttg gcgatttgtt
    10141 ctatggagta tatctacttt gctcgccctg attctaatat ccacggtgtc aatgtccata
    10201 cggcacgtaa gagaatggga gcgcaattgg cgcgagaatt taagcatgag gcagatattg
    10261 tagttggtgt gcccaattct tccctaagcg cggctatggg atttgcggaa gaatcaggct
    10321 taccaaatga aatgggtctg atcaaaaacc aatacaccca gcgaactttt atccaaccga
    10381 ctcaagaatt gcgggagcaa ggagtgcgga tgaaactgtc tgctgtttcg ggtgttgtca
    10441 aaggcaaacg tgtggtcatg gtggatgatt ccattgtacg tggaacaacc tctcgtcgta
    10501 tcgttcagct cttgaaagaa gcgggtgcga ctgaggttca cgttgccatt ggaagtcctg
    10561 cactagcgta tccatgtttc tacgggattg atatccagac ccgtcaggag ctgattgcag
    10621 ccaatcatac ggtcgaagaa actcgccaaa tcattggtgc ggacagtatg acttatcttt
    10681 caattgatgg cttgattgag tcgattggta tcgaaacaga tgcgccgaac ggtggtctct
    10741 gtgtcgctta ctttgacggt gactacccaa cgcctctcta tgactacgaa gaagactatc
    10801 gtagaagttt ggaagaaaag accagttttt acaagtaggc gacagattct ccattaaaga
    10861 aaaggaaaaa acaaatgaca aataaaaatg catatgccca gtcgggtgtg gatgttgaag
    10921 cgggttatga agttgttgaa cggattaaaa agcacgtggc ccgtacggag cgtgcaggtg
    10981 tcatgggagc tcttggtggc tttggtggta tgtttgacct ttccaagact ggggttaaag
    11041 aacccgtctt gatttcaggg actgacggtg tcggaaccaa gctcatgttg gctatcaagt
    11101 acgacaagca cgataccatc gggcaggact gtgtggccat gtgtgtcaac gacatcattg
    11161 ctgcaggtgc ggaacccctc tattttctcg actacgtagc gacagggaag aatgaaccag
    11221 ctaagctaga acaagtggtt gctggtgtgg cagaaggttg tgtgcaggct ggtgctgccc
    11281 tcatcggtgg ggaaacggct gaaatgccgg gcatgtacgg cgaagacgac tatgacttgg
    11341 ctggttttgc ggtcggtgtg gctgaaaaat ctcaaatcat tgacggttca aaggtggtag
    11401 agggagatgt tcttctcgga cttgtttcaa gtgggattca ctcaaatggt tactctttgg
    11461 ttcgtcgtgt ctttgcggat tacacaggtg aggaagtcct accagaattg gaaggcaaga
    11521 aacttaagga agttctactt gagccgactc gtatctatgt caaggctgtc ttgccgctca
    11581 tcaaagaaga gttggtcaac ggcattgccc acatcacagg tggtggcttt atcgaaaatg
    11641 tccctcgtat gtttgcagat gacctagctg ctgaaattga tgaaagcaaa gttccagtgc
    11701 ttccaatttt caaagccctt gaaaaatacg gtcagattaa acacgaagaa atgtttgaaa
    11761 tcttcaatat gggtgtggga ctcatgttgg cggtcagccc tgaaaatgta gagcgtgtaa
    11821 aagaattgtt ggatgaagca gtctatgaaa ttggtcgcat cgtcaagaaa gaaaacgaaa
    11881 gtgtcattat caaatgaaaa aaatagcggt ttttgcctct ggtaatggct caaattttca
    11941 ggtgattgcc gaagaatttc cagtggagtt tgtcttttca gaccatcgtg atgcctatgt
    12001 gcttgagcgt gcaaagcagc tcggcgttct gtcctatgct tttgaactca aggagtttga
    12061 gagcaaggca gactacgaag cagcccttgt cgaactcttg gaagaacacc agattgactt
    12121 ggtttgccta gcaggctaca tgaaaatagt tggaccaacc ttattgtcgg cttatgaagg
    12181 tcggattgtc aacattcatc cagcctactt gccagaattt ccaggagctc atgggattga
    12241 ggatgcttgg aatgctggcg tgggtcagtc tggtgtgacc attcactggg tggattcggg
    12301 tgtggataca ggccaggtca tcaaacaggt tcgtgtgcca cgactagctg atgataccat
    12361 tgacagattt gaagctcgca tccatgaagc agagtacagg ttgtatccgg aagtagtgaa
    12421 ggctctattt acagattgac tttttgatga ttcatatgat atctttgatt ttaaattgga
    12481 gtcagtgttt gttgaagacg gcttcaaacg gaggtatttg taatgttaga atctaaaaaa
    12541 acaactcgat atgtatttta tgtctatctg atgttattaa cttggggaat cttatttaag
    12601 tttgaaacaa atcctgaatt tatagcattt ttcttagctc caaggtatat caattggatt
    12661 ccattttcag aaccactaat agtcgatgga aaaattgttt ttgctgaaat gttatttaat
    12721 ctgatttcct ttattccatt aggtgtttgt ttccctttga taaaaactaa tttatctagt
    12781 ttaagaatag tcgggacagg tttcttgatt agtttattgt ttgagtgctt acagtatatt
    12841 ttagcaatag gtataacaga tataacggat ttgactttaa atacgctagg tgtctgtgta
    12901 ggcttactga tttatcaaat ttttataaga gtgttcaaat cacagactag aaaatggatc
    12961 aatatcttag gtatgcttag ccttggtttt gcttatcttg ttttactgtt actgcattta
    13021 attggtgttt aactaatgat taaaaaggag aatataatga ctaaacgcgt cttaatcagc
    13081 gtctcagaca aagcgggcat tgttgaattt gcccaagaac tcaaaaaact tggttgggag
    13141 attatctcaa caggtggaac taaggttgcc cttgataatg ctggggtgga taccattgct
    13201 atcgatgata tgactggttt cccagaaatg atggacggtc gtgtgaagac actccaccca
    13261 aatatccacg gagggcttct cgctcgtcgt gacttggata gccacttgga agcggctaag
    13321 gacaacaaga ttgagctcat tgaccttgtg gtggtcaacc tttacccatt taaggaaact
    13381 atccttaaac cagatgtgac ttatgctgat gcagttgaaa atatcgatat tggtgggcca
    13441 tctatgcttc gttcagcagc gaaaaatcat gccagtgtta cagttgtggt agatcctgct
    13501 gactacgctg tggttttgga cgagttgtca gccaatggtg aaacgactta tgaaactcgc
    13561 caacgtttag cagccaaagt atttcgtcac acagcggctt atgacgcctt gattgcagaa
    13621 tacttcacag ctcaagtggg tgaaagcaag cctgaaaagt tgaccttgac ttatgacctc
    13681 aagcaagcca tgcgttacgg tgagaaccct caacaagacg cggatttcta ccaaaaagcc
    13741 ttgccaacag actactccat tgcttcagcc aaacagctca atggtaaaga attgtccttc
    13801 aacaatatcc gtgatgcgga tgctgccatt cgtattattc gtgatttcaa agaccgtcca
    13861 actgttgtgg ctctcaaaca catgaaccca tgtggaatcg gtcaggctga taacattgag
    13921 actgcttggg actacgctta tgagtctgac ccagtgtcta tctttggtgg gattgtcgtc
    13981 ctcaaccgtg aggtggatgc tgcgacagct gagaagatgc acggcgtttt cctcgaaatc
    14041 atcattgcac caagctatac ggatgaagcg ctagctattt tgatcaataa aaagaaaaac
    14101 ttgcgtatcc ttgccttgcc atttaatgct caagaggcta gcgaagtgga agcagaatac
    14161 acaggtgtag tcggtggact tctcgtgcaa aatcaagacg tggtcaagga aagcccagct
    14221 gactggcaag tggtgaccaa acgtcagcca actgagacag aagcgactgc tcttgagttc
    14281 gcttggaagg ctatcaagta cgtcaaatca aatggtatta tcgtgaccaa cgaccacatg
    14341 acacttggtg ttggtccagg tcaaaccaac cgtgtggctt ctgttcgcct tgccattgac
    14401 caagccaaag atcgtctgaa cggggcggtc cttgcttcag atgccttctt cccatttgcg
    14461 gataacgtgg aagaaatcgc caaagcagga attaaggcca tcatccagcc cggtggctct
    14521 gtccgtgacc aagaatccat cgaagcagcg gataaatacg gcttgactat ggtctttaca
    14581 ggtgtgagac attttagaca ttaagaagat aaaagggaag aaaacagttt ctttcctttt
    14641 ttggcttaaa atgctaactg aaacaagatt aaaacgaact tttttgatat aatattggta
    14701 aataattcgc aaaagaggtt gaggaatgaa actgcttgtt gtcggttcgg gtggtcgtga
    14761 gcatgcgatt gctaaaaagt tacttgaatc aaaagacgtt gaaaaagtct ttgtagctcc
    14821 tgggaatgat gggatgactc tggatggttt ggaattggta aatatctcta tttccgaaca
    14881 ttctaaattg attgacttcg caaagaccaa tgatgttgct tggaccttta tcggtccaga
    14941 tgatgccctt gctgctggta tcgtggatga ttttaaccaa gctgggctta aggcctttgg
    15001 tccgactagg gctgcagcgg agctggagtg gtccaaggat tttgccaagg aaatcatggt
    15061 caaatatggt gtttcgacag cagcctatgg cacattttca gatttcgagg aagcaaaggc
    15121 ctatatcgaa aagcatggtg cgcctatcgt agtcaaggcg gatggcttgg cgcttgggaa
    15181 gggtgtcgtc gtttctgaga cggttgagca agcggtcgaa gccgctcatg agatgctttt
    15241 ggacaataaa tttggtgact caggtgcgcg cgtggttatt gaggaattcc ttgaaggaga
    15301 ggaattttca ctctttgcct ttgttaatgg tgataagttc tacatcatgc caacggctca
    15361 ggaccacaaa cgtgcctacg atggcgacaa agggcctaac acgggtggta tgggtgccta
    15421 tgcgccagtt ccacacttac cacagagtgt agttgataca gcggttgaca ccattgtcaa
    15481 gccagttcta gaagggatga ttaaagaagg tcgcccttat ctgggagttc tttacgcagg
    15541 gcttatcctg acagctgatg gaccgaaagt cattgagttc aacgctcggt tcggagatcc
    15601 agaaactcag attatcttgc ctcgcttgac ctctgacttt gctcaaaata tcacagatat
    15661 cctggatagc aaggagccaa atatcatgtg gacggacaag ggtgtgactc tgggtgtggt
    15721 tgtcgcatcc aagggctacc cgctagacta tgaaaggggc gttgagttgc cagccaagac
    15781 agaaggcgat gtcatcacct actatgcagg ggctaagttt gcggaaaata gcagagcact
    15841 gctctcaaac ggcggacgag tttatatgct cgttaccaca gcagataccg tcaaagaagc
    15901 ccaagccagc atctaccaag aactatacca acaaaaaata gaaggactct tctaccgaac
    15961 agatatcgga agcaaggcaa ttaagtaaag atataagaat aacgcgccgt agtcgccaaa
    16021 cacgataatg gtcgtcgtgg tgaaaagacc agaacagtga atgttctggt cagggggaaa
    16081 cttggagacc gtaggctcaa agtttaggaa tgaaaccgaa ggtttgcttc cgcctccatc
    16141 acctaagacc attatcaaaa agaaaaataa aaattcacaa aatacgttaa tgatcgtatg
    16201 gtttgcgagc gttagcgagc taatatagaa caatcaccgc cgttgtgaaa gaacgattgg
    16261 atgataatcc aatcgttcag ggaaattgga agaccttggg tttccaattt aggcatgaga
    16321 cacctttggt ggctgctgcc gtccctcaca agctaaggtg attgttgaaa aagaggaaaa
    16381 aggagaagaa atgaaaccag taatttccat catcatgggc tcaaaatccg actgggcaac
    16441 catgcaaaaa acagcagaag tcctagaccg cttcggtgta gcctacgaaa agaaagttgt
    16501 ttccgcacac cgtacaccag acctcatgtt caaacatgca gaagaagccc gtagtcgtgg
    16561 catcaagatc atcatcgcag gtgctggtgg cgcagcgcat ttgccaggca tggtagctgc
    16621 caaaacaacc cttccagtca ttggtgtgcc agtcaagtct cgtgctctta gtggagtgga
    16681 ttcactctat tctatcgttc agatgccggg tggggtgcct gttgcgacca tggctatcgg
    16741 tgaagctgga gcgactaacg cagctctctt tgccctccgt ctcctctctg tagaagataa
    16801 gtccattgcg gatgcacttg ccaactttgc tgaagaacaa ggaaaaatcg cagaggagtc
    16861 gtcaaatgag ctcatctaaa acaatcggaa ttatcggtgg cggtcaactg ggtcagatga
    16921 tggccatttc tgctatctac atgggccaca aggttatcgc gctggatcct gcggcggatt
    16981 gcccggcctc tcgcgtggcg gaaatcattg tggcacctta taacgatgtg gacgccctcc
    17041 gtcagttggc agaccgttgc gatgtcctca cttatgagtt tgaaaatgtc gacgctgacg
    17101 gtttggatgc cgttatcaag gatggacaac tccctcaagg aacagatctg ctccgcattt
    17161 cgcaaaatcg tatttttgaa aaggactttt tgtcaaacaa ggctcaagtc actgtggcac
    17221 cctacaaggt cgtgacttct agcctagact tggcagatat cgacttgtcg aaaaactatg
    17281 tcctcaagac tgcgactggt ggctacgatg gtcatggaca aaaggttatt cgttcagaag
    17341 cagacttgga agcagcctat gcgctagcag actcagcaga ctgcgtcttg gaagaatttg
    17401 tcaactttga ccttgagatt tctgtcatcg tgtcaggaaa tggcaaggag gtgacgtttt
    17461 tcccagttca ggaaaatatc caccgcaaca atatcctgtc taagaccatc gtaccagccc
    17521 gcatttctga aagtctagta gacaaggcta aagctatggc agtgcgaatc gcagaacaac
    17581 tcaacttgtc tggaactctc tgtgtggaaa tgtttgcgac tgatgatgac gttattgtca
    17641 atgaaatcgc cccacgacca cataactctg ggcactattc tattgaagcc tgtgatttct
    17701 ctcagtttga cacccatatt ctcggtgttc tcggagcacc attaccagtc atcaaactcc
    17761 atgcgccagc cgttatgctt aatgtcctcg gtcagcatgt cgaggctgct gagcaatatg
    17821 tgacagaaaa ttcaagcgcc cacctccaca tgtatggtaa aatagaagcg aagcacaacc
    17881 gcaagatggg acatgtgacc ttgtttagtg atgtaccgga tagtgtggat gagcttgggg
    17941 aagggattga tttttaggac aagtctatga tacaaattat cgttaataca tttattgaaa
    18001 aggataagac tggagcagtt gttgaagtgt tgtatgccag tgctgaccaa gataaggtaa
    18061 aagctaaata tgaagaacta gctgcacaat accccgaaaa ttatttagct atctatgatg
    18121 taccgctgga tacggatttg aatacactag atcattaccc gtctgtgttt attgaaaaag
    18181 aggagtttga gtagaaatct tggtttacct agatagctta ttcccaacag cttaagaaga
    18241 aaggaaaaat taacacatga tcaaccgtta ctctcgccct gagatggcga atatttggag
    18301 tgaagaaaat aaataccgtg cttggcttga ggtggaaatc ctctctgacg aggcatgggc
    18361 tgagttgggg gaaatcccta aggaagatgt ggctttgatt cgcaagaagg cggactttga
    18421 catcgaccgt attttggaaa ttgagcagga gacgcgccac gatgtggtgg ctttcacgcg
    18481 tgcggtttct gagactcttg gtgaagagcg caagtgggtt cactatgggt taacttctac
    18541 cgacgtggtg gatactgctt atggttacct ctacaagcag gccaacgaca tcatccgtcg
    18601 tgaccttgaa aacttcacta atatcatcgc tgacaaggcc aaggagcaca agttcaccat
    18661 catgatgggg cgtactcatg gtgtgcacgc tgagccgaca acctttggtc ttaaattggc
    18721 aacttggtac agcgaaatga aacgcaatat cgagcgtttc gagcatgcag ctgctggtgt
    18781 agaagctggt aagatttctg gtgcggttgg gaactttgcc aatatcccac catttgtaga
    18841 ggagtatgtc tgcgataaac ttggcatccg tgcccaagaa atctctacac aagtccttcc
    18901 tcgtgacctt cacgctgagt actttgcggt tcttgccagc attgcgactt caatcgaacg
    18961 tatggcgact gagattcgtg gtctacaaaa atctgagcaa cgcgaagtag aagagttctt
    19021 tgctaaaggg caaaaagggt cttcagcaat gcctcacaaa cgcaacccaa tcggttctga
    19081 aaatatgact ggtctggcgc gtgtcattcg tggtcacatg attacggctt atgaaaacgt
    19141 cgctctctgg cacgaacgcg atatttctca ctcatcagct gagcgtatca tcacaccaga
    19201 tacgaccatt ttgattgact acatgctcaa ccgttttgga aatatcgtca agaacttgac
    19261 agtcttccca gaaaatatga tccgaaacat gaactcgact tttggtctta tctttagcca
    19321 acgggctatg ttgacattga ttgaaaaagg catgacccgt gagcaagcct atgacttggt
    19381 gcaaccaaaa acagcctact cttgggacaa ccaagtagac tttaaaccac ttcttgaggc
    19441 agattcagaa gtaacatcac gtctcactca agaagaaatc gatgaaatct tcaacccagt
    19501 ttattacacc aaacgagtgg atgatatctt tgaacgtctt ggactaggtg attaattaaa
    19561 aaataaacag cgagcttcaa tctcgctgtt tattttttat cgaaaagact tagtcttctt
    19621 ttcttttagt gagtccatag gctgctagtg tggacatgag tcctgcgact actagtcctg
    19681 cagaatcgtg agttcctgtt tcaggaagtt ttttctctgt taccacagga gctggatctt
    19741 gaggaagagc tttgctttcc tcagcgggag cagttgatgg agctggttgg cttgggattt
    19801 ctagttttgg tttttcttca gcaatagcgg cttgtccgtt ttcatcgcct acatgtgtta
    19861 ccatagttcc gacttcgact atttgagtaa cggcttcctg tgctacgaca ctatttacaa
    19921 gtgttttcac ttccttacca tcggcagaag tgctcacaga gtagaagttg ctacgatgtc
    19981 cattgacgcc cttagtaatg acttgtgttt ttcctttgag taagagtgga ttttcacgag
    20041 tcactgtggt aaatggaatt tcttcttttt ggatatccag tctaggtttt acctcagtag
    20101 ttggtgcaag accactttca tcacccttgt gagttacagg agcgccaact tcaaccactt
    20161 ggtttataac ttctttggtt acctggctat caaggactgt ttctgttgtt tttccatttt
    20221 cagtgagtac agagatgtaa tgagttcgtt cacctttgac tcctgctgtg ataatatttt
    20281 cctgaccagc tgggaggtta ggattttctt tcttgataac ttcaaatgga atttcttcag
    20341 ttcttgtgat gagttctggt ctggtttcaa cattggcagc cacttcattt tcatccaggc
    20401 ttcctgaatg agttgcagct ggtttgaggc cttgaagagc ggcttttagg ttggctacaa
    20461 gtgtgtcaag ctcagcttgt ttattacggt tgaggttgta atttagagct gttttagctg
    20521 cgtcaagggc ctcaagactt tctttactat atccttctaa gtttgtagga attttagcta
    20581 attcttcgcg gagagcatta taattagcac ggaagtagtc tttgttgtgg tctgcaaagg
    20641 cagtcatgag ttcaaagatt tcctcttcct tgtactcagc gcttggtcta tctgcccaga
    20701 ttgaaagcat acttccgact gttggaagat ctacttcagg atatttggta gaagctagtt
    20761 gattgaatgg tgtttttcca gtattctcaa tagctttctt gaggaaacca ccaccatctt
    20821 ctggtttttg accaagaatg tagtaccagt caccgttggt attcaagaat ttatagcctt
    20881 tgcttgctag gtattgaggt gatgcgaggt tatatcccca ccagccttta gaccagtaag
    20941 aaatcaagac atctttgtca aactgaacat cgtccttgtc ttcatagtag aagccatcgt
    21001 tgaaggccat tggttgaagc cctctttctt tggccatagc tgcgagggtg ttggcatatt
    21061 cggcaaattt gccatagagt tgataccact tgaggtagta ccagccttgg gcactagtcg
    21121 catcgttggc gtattcgtca gtaccaaagt tgaaaatctt tgttttacct gcaaagaagt
    21181 ccatgtattt accgatgagg gcttttacaa agttcatcgc ttcttcgttt ttcaagtcca
    21241 tagttgtttt tgaaacttta tcaaagtggg cttgaggatt tttaatacct aatttttcca
    21301 tggcaaccag catagcatcc atgtgacctg gactgttaat agctgggatg agaccgatgt
    21361 ccttagattt agcgtattca attagctctg ttacttctgc ctgtgttagt gcagtaccgt
    21421 ttggatcgtc gtagtaagct ttagttcctt cgataatagc ttttttaacg tcatcactag
    21481 cataggtttt tccgttggca gtaatggtca tatcatcgag tagaaagcga agtccgtcat
    21541 ttcctagaag gagatggaca tcagaatatc cgagctcact ggccttgtct acgatgcgtt
    21601 tgagctggtt cagagtaaag tatttgcgtc cagcatcgat tgagattacc ttgtttttgg
    21661 caagtttttc aacctcacgt ttagcttctt cttcttttag agcttcaggc gtgagggtca
    21721 agttgttgac agtttcttga agtttagcaa tggcttgatc aatcgtatct tgttgggcac
    21781 ggctaaggtt gctatcgaga gagcgaatag ctttttcagc ttcttttacg gccgtgacgc
    21841 tttctgcagt ataacggttc aggtcttttg gtacctcgtt aagtgcttgc tctgcagatt
    21901 cataatcagc tgcgaagtat tcagcgttgg catttgcaaa atgacgcatg agtttgaaga
    21961 ggcgtgatgg tgaataacgt gcagatggag tgtcagccca agcagctacc ataccaccga
    22021 tgattgggat atcagctcct tctgtttttg gtacagaagt gattggtgtg tttttaatac
    22081 cattgagccc ctgatcgaga ttgtaccagc cttggccatc agcgtttcgt ccaagaacgt
    22141 agtaccaagc atcattggta ttaaggattt ggtgaccttt ttcagctagt agtttagaag
    22201 aagcgacatc gtagcctccc caaccaccag tccacataga aacgatgatg tctttgtcaa
    22261 aactaccaaa gcttgtgtcg ctattgtagt agataccgtc gttaaaagcc attggtttga
    22321 gaccgtgcga ttttacaata cgagcgaggt cattggcgta ggcaataaat ttttcatagc
    22381 cttttacagg gtagccttcg tttggatagt atttatcagc ttgaagcaca ctccaacctt
    22441 tagcatctgt cgcatcattg gcatattcat caagtccgat gttgaagatt tcagtctttt
    22501 tcgcgaaata agcagcatac ttgtcgataa gggcttttgt aaaagcgaca gcttgttcgt
    22561 tgtcaagatc gacagtacgg gctgatttct tcccaaaata gctaaagtta gggttttgga
    22621 ttcccaattc tttcatggca ttgagaatcg catccatgtg tccaggacta tttactgtcg
    22681 gaatgagacc gataccttta tctttggcat agttaatcag atctgtcatt tgactttctg
    22741 ttaagtgatt gccgtttgga tcgttgtaat aatcatttgt acctttttca atggcgcgtt
    22801 tgacatcgtc actggcatag gtcttgccgt tagctgtgat gctcatatcg tccaacatga
    22861 aacggagtcc atcatttccg actaataggt gtaaatcagt gtagccataa tgtttcgctt
    22921 tatcgatgat ttccttgagt tgttctggtg agaaatattt acgtccagca tcaatagaaa
    22981 caattttctt tttcgctagt ttttcattta cagttgcagc acgttccttt cctgcctctg
    23041 tttccggttt gtcagcctct gctttcgctt catctttttt agctggttta tccttgtcag
    23101 tcttgtctgt atttgactct ttagaatcaa cctctttcgc ttcttccttt ttagggctag
    23161 cttcttctgc ctttttatta gcagtttctt tttcagcaga agttggagtt accacttctg
    23221 ctttatcact aggagttgaa ctaacttcct cttgtggttt ttcttctgtt tttggaagac
    23281 tagctacctt atcagtagct ggagtttctg tttctacagt ttttggagct tctggttgaa
    23341 gcactgcttt aggtgtttcc tcagtccgat tttcggatga ttgaggggaa tcagaaaccg
    23401 tatggatggt cggttggttt tctgtagtag taggagtaac tccatcggct gcaacagtct
    23461 gtgcttggaa ggcaaatcca attagaacag aagctgctcc tacagcgtat ttacgaatag
    23521 aaaaacgctg ttgtttttca tgtttcattg caaaacctcc tgattgcatt gttatattga
    23581 tagcgattat ataaatcaac gcctttattt tatttcttat attaacgaga gtcaagagga
    23641 gatgacaaa
//
LOCUS       DBERCA010000023        22228 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.023, whole genome shotgun sequence.
ACCESSION   DBERCA010000023 DBERCA010000000
VERSION     DBERCA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.023"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            154..456
                     /locus_tag="ACL719_001090"
     CDS             154..456
                     /locus_tag="ACL719_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS0699073.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAVAGKTSEAWIWTKFGEQVSFFYDYDD"
     gene            527..2548
                     /locus_tag="ACL719_001091"
                     /pseudo
     CDS             527..2548
                     /locus_tag="ACL719_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            2550..3191
                     /locus_tag="ACL719_001092"
     CDS             2550..3191
                     /locus_tag="ACL719_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699074.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3295..4647
                     /gene="murD"
                     /locus_tag="ACL719_001093"
     CDS             3295..4647
                     /gene="murD"
                     /locus_tag="ACL719_001093"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGS0699075.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGEHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4651..5709
                     /locus_tag="ACL719_001094"
     CDS             4651..5709
                     /locus_tag="ACL719_001094"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGS0699076.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5719..6948
                     /gene="divIB"
                     /locus_tag="ACL719_001095"
     CDS             5719..6948
                     /gene="divIB"
                     /locus_tag="ACL719_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGS0699077.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEIEE
                     SESKEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     AFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKK
                     QEEESNRNQTTQRSSRR"
     gene            7181..7882
                     /gene="pyrF"
                     /locus_tag="ACL719_001096"
     CDS             7181..7882
                     /gene="pyrF"
                     /locus_tag="ACL719_001096"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGS0699078.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            7916..8548
                     /gene="pyrE"
                     /locus_tag="ACL719_001097"
     CDS             7916..8548
                     /gene="pyrE"
                     /locus_tag="ACL719_001097"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGS0699079.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            9227..9991
                     /locus_tag="ACL719_001098"
     CDS             9227..9991
                     /locus_tag="ACL719_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699080.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFLAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            9991..10743
                     /locus_tag="ACL719_001099"
     CDS             9991..10743
                     /locus_tag="ACL719_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699081.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLALLSIIIPLTVFSVHRNWRRV"
     gene            10753..11451
                     /locus_tag="ACL719_001100"
     CDS             10753..11451
                     /locus_tag="ACL719_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699082.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            11448..12095
                     /locus_tag="ACL719_001101"
     CDS             11448..12095
                     /locus_tag="ACL719_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699083.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(12182..13048)
                     /locus_tag="ACL719_001102"
     CDS             complement(12182..13048)
                     /locus_tag="ACL719_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0699084.1"
                     /translation="MKLFKPLLTVLVLAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKLFGYVDNDGSYQGYDIELGNQLAQNLGVKVKYISVDAANRA
                     EYLISNKVDIILANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDTAK
                     ADDLVVEGGKVD"
     gene            complement(13060..13817)
                     /locus_tag="ACL719_001103"
                     /pseudo
     CDS             complement(13060..13817)
                     /locus_tag="ACL719_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(13818..14495)
                     /locus_tag="ACL719_001104"
     CDS             complement(13818..14495)
                     /locus_tag="ACL719_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0699085.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(14455..15135)
                     /locus_tag="ACL719_001105"
     CDS             complement(14455..15135)
                     /locus_tag="ACL719_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0699086.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSGK"
     gene            15350..15484
                     /locus_tag="ACL719_001106"
                     /pseudo
     CDS             15350..15484
                     /locus_tag="ACL719_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            15624..17114
                     /gene="lysS"
                     /locus_tag="ACL719_001107"
     CDS             15624..17114
                     /gene="lysS"
                     /locus_tag="ACL719_001107"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGS0699087.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALCEQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKVIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            17738..18874
                     /gene="lctO"
                     /locus_tag="ACL719_001108"
     CDS             17738..18874
                     /gene="lctO"
                     /locus_tag="ACL719_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGS0699088.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      19126..19214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19429..20097
                     /locus_tag="ACL719_001109"
     CDS             19429..20097
                     /locus_tag="ACL719_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGS0699089.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(20094..20321)
                     /locus_tag="ACL719_001110"
     CDS             complement(20094..20321)
                     /locus_tag="ACL719_001110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699090.1"
                     /translation="MKIKEQTRKLTADCSKHCFEPVDKTDEVSSKHCFEVVDRTDEVS
                     NHTYGKVKLTWFEEIFEEYYLIKFVNIMSLL"
     gene            20424..21206
                     /gene="thiM"
                     /locus_tag="ACL719_001111"
     CDS             20424..21206
                     /gene="thiM"
                     /locus_tag="ACL719_001111"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS0699091.1"
                     /translation="MTSLKLLKKKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGMDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            21208..21837
                     /gene="thiE"
                     /locus_tag="ACL719_001112"
     CDS             21208..21837
                     /gene="thiE"
                     /locus_tag="ACL719_001112"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS0699092.1"
                     /translation="MFHKELLKLYFICGTTTCQGKDLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      22046..22134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         6802 a         3835 c         4789 g         6802 t
ORIGIN      
        1 taaaatatct aaaaaattcg attttttcct tgacaacaaa taataaaaat attattctta
       61 tcacataaga aagcggtttc aataaaagag agaggaggat ttattttgtt gcattgttgc
      121 tcagggtgtg ctttttttga aagaaaaaat aaaatgaaaa cgaaaaaaca tagattactt
      181 gctctagctc ttatttcaag ttttacatta ttgggagctg catcagctgc tgtacaatat
      241 ccagatggag gagtatggac atatggagaa ggttcaggag gtggttgggc tttttcaaat
      301 tactatcatg gtaaaaaata tcattattca tccatcgtga gcagatggga tggtcattct
      361 gataaaggag aagcagttgc aggaaaaact tcagaggctt ggatttggac taaatttggg
      421 gaacaagtat catttttcta tgactatgac gactaatatt tagttttctt agataattcc
      481 ccttgtaaga aattacaggg ggaattttgt ttacaaggag tctactatga agaaactatt
      541 catattatta tcaacttttt ttctcagctt cttccttgct tggattattg tcttacgtgc
      601 gccacaatat ttatatgcaa gctatgattc cgtttcttta cttcgtgtca aaaaagatac
      661 tcaggaaccg acgcgtgagg tatttgaaca ggaattggag aattttgcaa actcagaaca
      721 gagtttaata gctagaagaa ttgtagagcc gagtaaggat gggacgactc actttactta
      781 tgcaacttat ggtcagggaa ctttaccaaa agaattccaa gaagctagcc aagaaagtcg
      841 tgaacgtagt gatccgctaa atagttatct ccttttgtca ggctccttga cgaaagaaaa
      901 gcttgccgat aaattaggag atttgggtta taaagcaagt gctgaccgaa agataccgcc
      961 ctattttctt gcttttcgaa tattactaaa tccccttatt ttaattagtt tagcaatatt
     1021 tggcttatct ttctttgctt tagtgattat cactcggatt aaggaaatga gagcagcagg
     1081 tataaaactc ttttctggtc agactctctt atccatcatg ggggattctt tatctactga
     1141 tatcaagtgg ctccttctat cagccctcct ttccttccta ggtgggggag tcgttctttt
     1201 tagtcaaggt ttgttttatc ctatcttgtt agccacctat ggttttggga ttagtttcta
     1261 tctgttgttt ttattggcga tttcaatttt actaatgctt ctttatctaa tgagtttgag
     1321 ttacaaagca ttagttcccg ttattaaggg gagattaccc cttaaacgcc tgatgatttt
     1381 aaccctattg tgtcagttgg ttgctgtctt tacagtagga tacgctgtta agacaggttt
     1441 gacgtcttac caacgattga aagaacttga aatttcaaaa caagcatggc aggatagagc
     1501 agactattat caaatttctt ttggcttagg tgatagagga aaagatacag aaaatcagag
     1561 caagtgatat gcctttgcca aggaagcaat cgaagaagaa caagctcttt atgtaaagga
     1621 taatctgctc cattttgcca atccacaagg aaaaaatgaa cagggagaga cactggatac
     1681 ctatagtcca gatgctaata cgctctatgt tagtcccagt tatttggaca aggaaaaggt
     1741 cgtggtagat gctgagacca aacagaagtt agcccatctt caaaaaggtg agtttatcct
     1801 cttgctccca gaacatttgc gctctcgaga agcagaactt aagaaagttt ttgaagaaag
     1861 attgagttat tatggaaaat ctggtgagga ggcaagtgct cctttggatt atgagatgaa
     1921 agcgcacgtt agttatcttt caatgggaga aaagcggttt gtttataata acggtgagaa
     1981 tcccgtatcc actcagtatt tgactgatcc gattttagtg gtattcacgc cgacttctac
     2041 aggtgatagt tttatatcct tatctagttg gtctatcaat gctggaaaac aactctttat
     2101 caaaggatat gagagtgggc tagaactctt gaagaaagct ggaatttatg agcaagtatc
     2161 ctatcttaaa gaaggaagaa gtgtttatct aactcgttat aatgaagttc aaactgaaac
     2221 agcaacttta atcttaggag ctattgttgg aatagccagt tccttgttac tcttttattc
     2281 tgtcaatctt ctatatttcg agcaattccg ccgagatatc ttgattaaac gaatttcagg
     2341 tttacgattt tttgaaacac atgctcagta tatggttagc caatttgcca gttttgtatt
     2401 tggtgctagt ctctttattt taagcagtcg agacttggtg attggcttgc tcactttatt
     2461 ggtctttcta gctagtgcag ttttgacgct ttaccgtcaa gcgcagaaag aatctcgtgt
     2521 ttctatgaca attatgaaag gaaaatagga tgattgaact aaagaatata tctaaaaaat
     2581 ttggaagccg tcagctattt tcagatatga atcttcattt tgaaggtggg aaaatttatg
     2641 ccttaatcgg tacaagtggc tgtggtaaga caacactctt gaatatgatt ggacgattag
     2701 agccatatga caaagggcaa atcatatatg atggcacttc tcttaaggac attaagcctt
     2761 ctgttttctt tagagattac ttaggatact tatttcaaga ttttggctta attgaaagcc
     2821 aaaccgtcaa agagaatctc aatctgggtt tagttggtaa aaagttgaag gaaaaagaga
     2881 aaatctcttt gatgaaacaa gctctaaacc gtgtaaacct ctcttatttg gatttgaagc
     2941 aacctatatt tgagttatca ggaggagaag cacaacgtgt tgcactagcg aagataattt
     3001 taaaggatcc acctttgatc ctcgcagatg aaccaaccgc ttccttagac cccaaaaact
     3061 ctgaggaatt actttccatc ctagaatctt taaaaaatcc gaatcggacc attattattg
     3121 cgacccataa tcctctgatt tgggagcaag tggatcaggt cattcgagtt accgatttat
     3181 cacatagatg atatggtaag attcagttag aagaaagagt cacaaacaca ctttgtggct
     3241 tttttatttc cataaaaatg gtaaaatagt aggagtagaa atggagttcg agacatgaaa
     3301 gtaatagatc aatttaaaaa taagaaagtc cttgttttag gtttggccaa gtctggtgaa
     3361 tctgcagctc gtttgttgga caagctaggt gccattgtga cagtaaatga tgggaaacct
     3421 ttcgaggaca atccagctgc ccaaagtttg ctggaagaag ggatcaaggt cattacaggt
     3481 ggccatcctt tggaactctt ggatgaagag tttgccctta tggtgaaaaa tccaggtatc
     3541 ccctacaaca atcccatgat tgaaaaggct ttggccaagg gaattccagt cttgactgag
     3601 gtggaattgg cttatttgat ttcagaagca ccgattattg gtatcacagg atcgaacggt
     3661 aagacaacca caacgactat gattggggaa gttttgactg ctgctgggga acatggtctt
     3721 ttatcaggga atatcggcta tccagctagt caggttgctc aaatagcatc agataaggac
     3781 acgcttgtta tggaactttc ttctttccaa ctcatgggtg ttcaagaatt ccatccagag
     3841 attgcggtta ttaccaacct catgccaact catatcgact accatgggtc attttcggaa
     3901 tatgtagcag ccaagtggaa tatccagaac aagatgacag cagctgattt ccttgtcttg
     3961 aactttaatc aagacttggc aaaagacttg gcttccaaga cagaagccac tgttgtacca
     4021 ttttcaacac ttgaaaaggt tgatggagct tatctggaag atggtcaact ctacttccgt
     4081 ggtgaagtag tcatggcagc gaatgaaatc ggtgttccag gtagccacaa tgtggaaaat
     4141 gcccttgcga ctattgctgt agccaagctt cgtggtgtgg acaatcaaac catcaaggaa
     4201 actctttcag ccttcggtgg tgtcaaacac cgtctccagt ttgtggatga catcaagggt
     4261 gttaaattct ataacgacag taaatcaact aatatcttgg ctactcaaaa agccttgtca
     4321 ggatttgaca acagcaaggt cgtcttgatt gcaggtggtt tggaccgtgg caatgagttt
     4381 gacgaattgg tgccagacat tactggactc aagaagatgg tcatcctggg tcaatctgca
     4441 gaacgtgtca aacgggcagc agacaaggct ggtgtcgctt atgtggaggc gacagatatt
     4501 gcagatgcga cccgcaaggc ctatgagctt gcgactcaag gagatgtggt tcttcttagt
     4561 cctgccaatg ccagctggga tatgtatgct aactttgaag tacgtggcga cctctttatc
     4621 gacacagtag cggagttaaa agaataaaat atgaaaaaaa ttgtctttac aggtgggggg
     4681 acggttggac acgttaccct caatcttttg ttaatgccca agttcatcga agatggttgg
     4741 gaagtccact atatcgggga caagcgtggt atcgaacacc aagaaatcct taagtcaggt
     4801 ttggatgtca ctttccactc cattgcgact gggaaattgc gtcgctattt ctcttggcaa
     4861 aatatgctgg acgtcttcaa agttggctgg ggaatcgtcc aatcgctctt tatcatgttg
     4921 cgacttcgtc cacagaccct tttttcaaag ggaggctttg tctcagtacc gcctgttatc
     4981 gctgcgcgtg tgtcaggagt gcctgtcttt attcacgaat ctgacctgtc tatgggcttg
     5041 gccaataaaa tcgcctataa atttgcgact aagatgtatt caacctttga acaagcttcg
     5101 agtttggcta aggtcgagca tgtgggagcg gtgaccaagg tttcggacaa aaacactcca
     5161 gaaccagatg aattggtgga tattcaaacc cactttaatc ccaaattgcc aactgtattg
     5221 tttgtcggcg gttctgcagg cgctcgtgtc tttaaccaat tggtgacaga ccataagaaa
     5281 gaactaacag agcgctacaa tattatcaat ttaactggag attctagcct gaatgagttg
     5341 aggcaaaatc tttttcgtgt tgactatgtg accgatctct atcaaccctt gatggaattg
     5401 gctgatattg ttgtgacacg aggtggtgcc aatacgattt ttgagctctt ggcgatagca
     5461 aaattgcatg tcattgtgcc gcttggtcgt gaagctagtc gtggtgacca gattgaaaat
     5521 gcagcttact ttgtaaaaaa aggctatgca gaagaccttc aagaaagcga tttgaccttg
     5581 gatagtttgg aagagaagct ttctcactta ctaagtcaca aggaagatta ccaagctaag
     5641 atgaaagctt ctaaggaatt gaaatctcta gcagattttt atcaattgtt gaaaaaagat
     5701 ttatcataag gaaagtaaat gtcaaaagat aagaaaaatg aggacaaaga aatcctcgaa
     5761 gaattgaaag agttatcaga atggcagaaa cgaaaccaag aatatctaaa aaagaaggct
     5821 gaagaagagg cggctctagc tgaggagaag gaaaaggaaa gacaagctcg aatgggagaa
     5881 gaatctgaga agtcagagga taaacaggac caggagagtg aaacagacca ggaagattca
     5941 gaatcagcta aggaagagtc tgaagaaaaa gtggcatcct cagaggctga caaagagaaa
     6001 gaagaaaaag aagagataga agaatcagag tctaaagaga aggaggaaca ggataaaaag
     6061 cttgctaaaa aggctataaa ggaaaaacca gccaaagcaa agattcctgg tatccatatc
     6121 ttgcgagcct tcacgatttt atttccaagt ctgcttttat tgattgtctc tgcctacttg
     6181 ctcagtcctt atgcgaccat gaaagatatt cgtgttgagg gaacggtgca aactacagct
     6241 gatgatattc gacaggcttc aggcattcag gattcggatt atacgattaa ccttctgcta
     6301 gacaaggcaa aatatgaaaa gcagattaag tctaactatt gggttgaatc agctcaactt
     6361 gtctatcaat ttccaactaa gttcactatt aaggtcaagg aatatgatat tgtggcctac
     6421 tatatttctg gtgaaaatca ttatcctatt ctttccagtg gtcagcttga gactagttct
     6481 gtgagcctga acagtttacc agaaacttat ttatcagttc tctttaatga tagtgaacaa
     6541 atcaaggctt ttgtctcaga acttgctcaa attagcccag aactcaaggc ggctatccaa
     6601 aaggtggaat tagccccaag caaggtgaca tccgatttaa ttcgattgac catgaatgat
     6661 tcggacgaag tcttggttcc tctatctgaa atgagtaaga aactgccata ttacagtaag
     6721 attaagcctc aattgtcaga accgagtgtg gtcgacatgg aagctggaat ctacagctac
     6781 actgtggcgg ataaattaat tatggaggct gaggaaaaag ccaaacaaga ggccaaggaa
     6841 gctgagaaaa aacaggaaga agaacagaaa cgtttagaag aagaacagaa aaaacaagag
     6901 gaagagagca atcgaaacca aacgacccag cgctcatcgc gtcgctaggt ttaccttttc
     6961 tcttatagtt ctttagtgac catgttttta cttgacaagt gctagtagaa ataatagaat
     7021 agtaaaaaac ctttaaagca gtccagagag gcagctaagg ttagacggtg aaagggtgga
     7081 gactacccat ttttcgtgga accttgctgt tggcaggttc cttttttcgt ggcttctgtt
     7141 ggccagactc tctcactagt aaaggtaaaa ggagaaacct atgcgagaac atcgtccaat
     7201 cattgctctt gattttccta gttttgaggc ggtcaaggaa tttttagctc ttttcccagc
     7261 agaagaaagc ctttatctca aggtagggat ggagctttat tacgcagcgg ggcctgagat
     7321 tgtgtcctac ttaaaaggtt tgggtcatag tgtctttttg gatctcaaac ttcatgacat
     7381 tcctaataca gtcaagtcag ccatgaaggt cttgtctcag cttggtgtcg atatgactaa
     7441 tgtccatgcg gctggtggtg tagagatgat gaaggcggcg cgtgaaggtc ttgggagtca
     7501 agccaaattg atcgctgtaa cgcagctgac atcaacgtca gaagcccaga tgcaggagtt
     7561 tcaaaatatc caaaccagtc tgcaagagtc tgtgattcac tatgccaaga agacagctga
     7621 agctggcttg gatggtgttg tttgctcggc tcaggaagta caagtcatca agcaggctac
     7681 caatccagat tttatctgtc tgacaccagg gattcgtcca gctggtgttg cagttggaga
     7741 tcaaaaacga gtcatgacac ctgctgatgc ctatcaaatc ggcagtgact atatcgtagt
     7801 gggacgtccc attacccaag ctgaggatcc tgttgcagct tatcatgcca tcaaggatga
     7861 atggacacag gactggaatt aaagaactag attagaaaaa taaaaggaga ataccatgac
     7921 acttgctaaa gatatcgcta gccacctctt gaaaatccaa gccgtttacc tcaaaccaga
     7981 ggaacccttc acttgggcat ctggtatcaa gtcaccgatt tacactgata atcgtgtgac
     8041 actagcctat ccagaaactc gtaccctaat tgaaaatggt tttgtggaag ctatcaaaga
     8101 agcctttcct gaagtagaag tgattgcagg aactgcaaca gcagggattc cacacggagc
     8161 cattattgct gataagatgg acttgccttt tgcctacatc cgtagcaaac caaaagacca
     8221 cggagctggt aatcaaatcg aaggtcgcgt agctcaaggt caaaaaatgg tagtggttga
     8281 agaccttatt tcaacgggtg gttcagttct tgaagctgta gcagcagcca agcgagaagg
     8341 agcagatgta cttggagttg tagcgatttt cagctaccaa ttgccaaaag cagataagaa
     8401 ctttgcagat gctggtgtta aacttgtgac gctttcaaac tatagcgagc ttatccatct
     8461 agcccaagaa gaaggttaca tcacgccaga gggccttgat cttctaaaac gctttaaaga
     8521 agaccaagaa aattggcaag aaggttaggt cagtaagata aagagagacg aggctaccga
     8581 gtctctttta ccattttatt taaaatatga caatatttta aataaaataa ttgacaacgc
     8641 tttcttttat tgatataatg aaaatgtaat atgtatcaaa ttgatcaatc aactatcttg
     8701 tttatcactt cttaagagga cgatgtcatg aaaagaagaa aaatatattc cttttttgcg
     8761 tagttacaat tgcatctagt ttgtttctcc ctacagtttt agctagacag attggaaatt
     8821 atgatttaac gttgccgcgt tggggttcgg atacaactag tgagcttgag aaagaaaacg
     8881 cctctgctgg aattaataat agtgacagca ctggtggcgg taaaaggtta agtacctcta
     8941 ttcgtagcgc ctatagtggg tcagatatta ccccggtata ttcattgggg tctggctcta
     9001 ggattgtcat gtactataat ggaggtggtg acaattatat tggttctggt actagattag
     9061 ctatggcgcc acaatttgga aatcatgtaa gaattcatac ttcaggttct tggagtccag
     9121 attcttatta acttacttgt cagagtaagc cttaaagatg gttgattgtg ggtgtagcat
     9181 gaaaaaagaa tgctacaccc tatttttatt ataaggagga gtaaggatgg aatttttcat
     9241 ttgtaatctt gtacgagtcg ttcaatcacc tcgattttat atgtctttat ttttgaccct
     9301 tctttgcatg agtttaggaa atttccttgc tttcaatggt atttataaaa ttgaaggttt
     9361 atcgattttt tttgccgctt cttctattcg aggattttca ccgattagcc tagtagctgc
     9421 acttatctgt acactgccct attctagtca gataatagag gatgctgaga gtcattttct
     9481 aacagcacaa ttgtgccgaa tttctaaaaa gaggtatcta gctattgtag gtagtactgc
     9541 aattatttct tcttttctag tcttttttct cccctattta ttattattag gaattaatct
     9601 tttagtgact ccttatcagg aaatttatat tggagattat agtggtgcct taaaagaatt
     9661 atttgattct aatcagttgc tctatagtct tgtgacgact ctctggtatg gagtttgggg
     9721 cgctgtgttc tctatttttg gactagctag tgctttgcta gtgaagaaaa aaataggagc
     9781 tattttcatc ccagttgcct atatgatggt tggtggtatt tttttggcta ttttagggct
     9841 atcttactta gaacctgtga caacattagc tttgggatat cagaaagata tcagtctttc
     9901 cttagttagt gggcatcttg cttttatttt atttgttagt tgtttggttg tttatggtac
     9961 attttttcta cattcagagg actatgtata atgaaacaat ttgttcaatt ttataaaaaa
    10021 gatttcttag cagtattggt ttattttata ttactgctat cctgtgtttt atctagtaca
    10081 gtatatttat tgcgctgtcg ccaatattca atccatccaa atgtattaga atggatctta
    10141 gttttacttc aagatatgac gactggagta tattgctttc cgttcacata tatattgttc
    10201 tttttttatt tgatgaataa ctattttaat aggttggagt gtcgcattcg tctgaaatca
    10261 attaagcact ttaccagttt tagtttcaaa ttagcagctc ttagtacggg gatttggacg
    10321 gcgattttat ttttattgat ttttctaatt gcatttagta atggttttag cttctctttg
    10381 gagataaagg aggttgattt tttaagagaa ttttatggca taagtattgc aaacaatgct
    10441 agtttcttta taggattttt tttctcttat atagcatact atttcttttt atccttactt
    10501 actattagca gtttttcttg gtttaaaaaa tcaaacatga gcttagtatt tctgtttact
    10561 tttttatttg tagaatcctt attctggatt tatcagttga acaatgggat aattggatta
    10621 ttgccaattt ttcagtatat ggtaaattcc aatccgtatg cattgattta ttggcttgca
    10681 ttactatcta tcataattcc attgactgta ttttctgttc atagaaactg gaggagagtg
    10741 taaaagttgg aaatgggaaa gttaagtagt cacatgtgga ggttgaatca gataatctat
    10801 accaagtact tttggggtta tgttcttttt tggatattga tttgtttagg attatggtat
    10861 tggttagaag gaaatgatag acttgttata gaaattttaa aagggcctaa tctgagtcaa
    10921 aactcttttt tagtcttatc tatatggttg cttcattggt ttattattca tacatttttt
    10981 ctagcagttg tatatcgtag aagagcatcc gatttctttc tggaagtgat tcgattttct
    11041 tctattaagc tctggattag gtatcagatt tggacctgtt ttctttatgg acttatttta
    11101 atcatggtaa aagttctagt gattcaattt atgttacagt taccaaactg ggatatagga
    11161 gttttgttta tagttgattc tttgaatgct tgtgtgctat ccttgctttg ctttatgtta
    11221 tacgcactag gagcgaatgt acaaatgaac tttgcttgcg ttagtttctt tttactcatg
    11281 attgtgtttg gtggtctatt tgtaggaaat cgaactaatt atctatttta tattttgaat
    11341 cgagggaatg gagatatagg acgagattta tttcttcagt tgctattttt agtctttctt
    11401 tttaaaagca ttttctattt cactcgtcaa aagaggaggt ttatagaatg aatgaaataa
    11461 ttacattaaa aaatattgag ttgaaattaa aaaaaacatg tgtttttcaa aaccttaatt
    11521 ttagttgtaa acagggggaa attataggaa ttactggtgc gaatggctca ggtaaaagtg
    11581 tattgtttaa attaatagct ggtttatata gtccgtctta tggagaagtg ttaatcaatg
    11641 gggaaaatat tgttcctgag agaaaaattc cagctaattt gggagctttg attgaagaac
    11701 ctggttttat aaattattat agtggcttta agaatttaca atatttggca agcatacgag
    11761 gagtagttgg taatcaggaa atcaatgata cactgaaaat agttggtcta tatgagcaaa
    11821 aagaccagaa agttaaaact tattcgctag gtatgaggaa aaagctaggg attgctcaag
    11881 caattatgga gaatccctct attcttttac tagatgaacc tatgaatgcc ttggataaat
    11941 caagtgtaga aaatatgaga acattgttta gaaagctctc tagtgaaaaa gggacaacaa
    12001 ttttgattgc tagtcatagt gaagaggata ttcgtatctt atgtgataaa gtatatgcaa
    12061 tagaagataa agtatgtaca ctgtgttcag attgaacatc ttagatagac tcaggatgtt
    12121 ataggtaaaa tcttaaaaag ggattggagc ttaaaatcca gttcctttta agagttaatg
    12181 actaatcaac ttttccacct tcaacaacca ggtcatctgc tttagcagtg tcaccgtagg
    12241 ttgggtgaag tgttttttca taggccttgt ggaagaagtt ttccttgcct aatttttcaa
    12301 tatctttatt gatgaagtct agcaattctt ggttgccttt ttgaactgct gccgcaatgg
    12361 tatcgggatc accgagggaa gtaattccta cttcaaatcc tttattttca agcgcccaag
    12421 ctagaacttc cgtattatca gttgaaaagg catctccacg tccgtcaaga agagcttggt
    12481 aagagtcact gtattggtcg tatttttgga gtttgatttc tggatgattc ttttcaaaat
    12541 aagtctcagc agtcgttcct tttgtgacaa ttaaggtttt accttcaagt tgtttgacgt
    12601 ctgtaatgag accagtctta ggtgatacga cacccagaga aactttcata tatggaaggg
    12661 caaaatcaac ttgtttctta cgttcgtcag ttactgtaaa gttagcaaga ataatatcta
    12721 ccttgtttga aatcaagtat tccgcacggt tggcagcatc gactgaaatg tatttaacct
    12781 tgacaccaag gttttgagct agttggttcc ctagttcaat atcgtagcct tggtaagaac
    12841 catcattgtc aacgtagcca aacagttttt tatctccaaa cacggcgatt cgcagttcac
    12901 cgcttttttt gatttcatcg atagtgcgag ctttggcagt tgtttttcca gaggatgaac
    12961 cagcgtttcc acctgagcta caagcagtga taaagataag ggcaaaggca agtactaaaa
    13021 cagttaagag tggtttgaat agtttcataa gaatctcctt tatagatatg agccgaattg
    13081 gctaaagtca aagacgttta aaaattcctg ggctcgtttg gtttgcggat tggtaaagaa
    13141 ggcttgagct gttccttctt cagcgatttt cccttggtcg aggaagataa tccggtcagc
    13201 aatggcttgg gcaaactgca tttcgtgggt tactaaaatc atggtacggc cttcttgggc
    13261 caaatcattg ataagttcca gcacctcacg caccatttct ggatccagcg aagcagtcac
    13321 ctcgtcaaaa aggatgattt ctggatgcat taggagggca cggacaattg caacacgttg
    13381 cttctgtcca ccagataatt gacgggcaaa gctatgttgt ttatccagca aaccgacacg
    13441 atctagtaat tgcaaagctt cttccgttac ttctttcttg tcccttcctt gagttttgat
    13501 agggcctagg atgaggtttt gtaagacatc cagatgggga aagagttcat aactttgaaa
    13561 gaccatgcca atcttttggc gaactaggtg aaaatctttt ttattttcaa cgatagactg
    13621 accatccaga aaaatatctc caccttgaat actttctaag ccgttgaggc aacgaaggag
    13681 ggtacttttc ccacaaccag atggccctag gatgacaaca acttcccttt tttgatttct
    13741 agagaaagtc ctttgaggat gggattgtct ccgaaggatt tttttagttc cttgatttct
    13801 aagatagttt cagacattta gtttctccaa tgtttttcta agtgagtgga tagtttggaa
    13861 atagggtagc aaactgcgaa atataagact agaatggttc cataaatcca aaatgaagca
    13921 gttgggatgg tcaggcgatt gctatcgatg atttgttgtc caactttggt cacttccaca
    13981 accccaatca aaacaactaa tgaagtggtt ttaatcatcc gagtgacaag attgatagcc
    14041 tgcggtagca gtcttcttaa gacttgtggg atgatgatgt ggtagtaaag ttgaacatta
    14101 gtcaagccga gtgcctgtcc actttcaaac tgatgtttag ggagagaagt gatagctcca
    14161 cgtaccaagt ctcccatttc agctgttccc cagagggtaa aaacgataat agctgaagtc
    14221 tcacctgaga tattgatatt aaagtttcga gccaagccaa agtaaacgat gaagagtagc
    14281 accagctggg gcatgatacg gataaattcc agatacaatc gtgttaaaaa tcgtatgatt
    14341 ctagaatggg aggtcatgat gattcccatg actgttctga acatcatgga taagaggaca
    14401 gacaggatgg atatctcaat cgtaacgccc aatccctgta agattctcag gagattattt
    14461 ccctgaaaga gtacttggat tcccgaatcc tgcatggcgg agcctccttt ctatccagct
    14521 aaagaccagt gagatgggta gcagcatgat cagataagca actaccaaca tagctagcgc
    14581 aatgtctgtc tcatagtaga gaccaatcaa atccttggcg acatacatga ggtcggccaa
    14641 agccactgct gagaaaacag aggtttcctt gataaggaaa atgacattgg cactaaagga
    14701 ggggagtgcc accgctgttg cttgcggaag aaccacatag cgaaagacct gtagaggtgt
    14761 cagaccaata gcgaggccaa tctcctgctg ggtttgactg atggcttcca gcccacttcg
    14821 gaaagattct gccatatagg agcctcctaa aaagacaagc ccaagcgttg cacagacttc
    14881 tgaagatagg acaatcccga ttcggggaag accgaagtag agaaagaaga gttgaatcaa
    14941 aaggggcgta ttacgtgaca attcaatgta ggctgtcgct acttgcgcca aaacagggat
    15001 tcgataatgt cggatgatac taacgattaa accgagcaga aaggatccca aaattcccca
    15061 aactgcaata tgcaaggtca gaaagaatgc cttttgatat agtggtagat attgttcaac
    15121 aatggaccaa tccaaaaata gaacctccca tctagaaata atacagttat tgtagcactt
    15181 aaaatcttct ttggataata tctatttttt attgccgtta taaggatttt ttaccataga
    15241 cataaaattt ctgaaatttc caaacaaaat atttgaaaag ttttgaaaaa gagttaagat
    15301 atttttgtaa tatacaaagt gaacgcttac ttattaagga gggcatttta tgtcatacaa
    15361 aacaagcaat gcagaaggtc atgtagattt catcaatacc tatgatttgg agccaatggc
    15421 gcaacaagtt attcctaaag cagcatttgg ctatatcgct agtggggcgg aagatacttt
    15481 cacttctttc cagtgatttt agcgtcaggt tctttttagt ttttaaagat tatccgtgaa
    15541 tttcttgctt atttatgata aaatgggggt atcgcaaaaa atgactcatc gtattcaatt
    15601 ttgagtaaaa ctaggaggat cccatgtcta cagaacatat ggaagaacta aatgaccagc
    15661 agatcgttcg ccgtgaaaaa atggctgcgc tctgcgaaca aggaatcgat cctttcggaa
    15721 aacgttttga acgtactgca aattcacaag aattaaaaga taaatatgcc aacctcgata
    15781 aagaacaatt acacgataaa aacgaaacag ctactatcgc aggacgcttg gtaaccaaac
    15841 gtggtaaagg aaaagttggt tttgcccacc ttcaagaccg cgaaggccag attcagatct
    15901 acgttcgtaa ggatgctgtc ggtgaagaaa actacgaaat cttcaaaaaa gcagaccttg
    15961 gtgacttcct tggtgtcgaa ggtgaagtga tgcgtacgga tatgggagaa ctctctatca
    16021 aggcaaccca catcacacac ttgtctaagg ctcttcgtcc tcttcctgag aaattccatg
    16081 gtttgacaga cgttgaaaca atttaccgta aacgttacct tgacttgatt tctaaccgtg
    16141 aaagctttga acgctttgtc actcgttcaa aaatcatctc tgaaatccgt cgttaccttg
    16201 accaaaaagg attccttgaa gtggaaacac ctgttcttca taatgaagcc ggtggtgctg
    16261 ctgcccgtcc atttatcacc caccacaatg cccaaaacat tgacatggtg cttcgtatcg
    16321 cgactgagct tcacttaaaa cgccttatcg tgggtggtat ggaacgtgtc tatgaaattg
    16381 gccgtatctt ccgtaacgaa ggaatggacg ctactcataa ccctgagttt acttctatcg
    16441 aagtttacca agcttatgca gacttccaag acatcatgga cttgacggaa ggcattatcc
    16501 aacacgctgc taaatcagtt aagggcgatg gcccagtcaa ctaccaaggt actgaaatca
    16561 agattaacga accatttaag cgtgttcata tggtggatgc tatcagagaa attactggtg
    16621 tcgatttctg gcaagacatg actttagaag aagctaaagt tatcgctgct gagaagaaag
    16681 ttccagttga gaaacactac actgaggttg gtcacatcat caatgccttc tttgaagagt
    16741 ttgttgaaga aactttaatc caaccaacct ttgtctatgg acatccagta gctgtatctc
    16801 cactcgctaa gaaaaatcct gaagaccaac gctttactga ccgtttcgag ctctttatca
    16861 tgactaagga gtacggtaat gcctttactg aactcaacga cccaatcgac caacttagcc
    16921 gttttgaagc ccaagctaaa gccaaagaac ttggtgatga tgaagcgaca ggaatcgact
    16981 atgactacat tgaagctctt gaatacggta tgccaccaac aggtggtttg ggaatcggta
    17041 tcgaccgtct ctgcatgctc ctcactgata caacaactat ccgtgatgta ttgctcttcc
    17101 caacaatgaa ataaattctt atcctctggc tcttaccaga ggattttttg attcaaaaag
    17161 agactgaatt taaggagaaa atgaagtgta gtatattgaa gttgaaatag tacactttga
    17221 tttctaagac attgttagaa attggtttaa attccctaag caatttgtgc atgttttatt
    17281 tcattttacg atagtacgct gaaactgttc aaaaagtact agaaattgac ttggattccc
    17341 caattgattt gttcagattc actataaata aaaaattaat aagtgggata ggaagttagc
    17401 gtcaactagg atagtatctt gcttaaacag tatatatggg actgatataa gtccataggt
    17461 cctattagag gatgttctgg tgtcttattc acttgttttt tatagtatta gtagatagaa
    17521 tcagcaaata aaaaagtagt tcaaatgcct ggcagcaaaa aatctcaacg attcacttaa
    17581 tatagactaa ggtttagagt ttaaggaaag tagggctagt atattgtatg aaaaaataag
    17641 atggttttta agcaaaactc ttgaaaatga ttgaaaaaag agttattata gttgtatagt
    17701 aaaaagcaaa cgcttactta atcgaggagg acagtttatg tcatacaaaa caagcaatgc
    17761 agaaggtcat gtagacttca tcaataccta tgatttggag ccaatggcgc aacaagttat
    17821 tcctaaagca gcatttggct atatcgctag tggggcggaa gatactttca ctttaagaga
    17881 gaatatccgt gcctttaacc acaagctcat cgttcctcat acactttgca atgtagaaaa
    17941 tccaagtaca gagattgaat ttgcaggtga aaaactatct tcaccaatca ttatggcgcc
    18001 tgttgcggcc cataaattag cgaatgaaca gggggaagtg gcgactgcgc gtggtgtgca
    18061 tgagtttggt tctctttata caaccagttc ttactctact gttgaccttc cagagatttc
    18121 tgaagccctt caagggacac ctcattggtt tcaattttac tttagtaagg atgacggtat
    18181 caaccgccac atcatggacc gtgtgaaggc tgaaggttat aaagcgattg tcttgacggc
    18241 agatgctact gtagggggga atcgtgaagt ggataagcgt aatggttttg tcttcccagt
    18301 tggcatgccg attgttgaag aatacctgcc agaaggtgct ggtaaatcaa tggactttgt
    18361 ttacaaatca gctaaacaac gcttgtctcc acgcgatgta gaatttatcg ctgaatactc
    18421 tggtcttcct gtgtatgtca agggaccaca atgccgtgag gacgttgaac gttcgcttgc
    18481 tgcaggagct tctggtatct gggtaaccaa ccacggtggt cgtcaaatcg acggtggacc
    18541 agctgccttt gactcgcttc aagaagtggc agaagcagtt gatagacgtg tgccgattgt
    18601 ctttgactct ggtgttcgtc gtggtcaaca cgtctttaaa gccttggctt caggagcaga
    18661 cttggtagct attggtcgcc ctgttattta tggcttggct ctcggtggta gtgtcggtgt
    18721 gcgtcaagtc tttgaacact tgaatgcgga attgaagaca gttatgcaat tgtctggagc
    18781 tcagaccatt gaagatgtca aacactttaa gctccgtcac aatccataca acccaacctt
    18841 cccagttgac cctcgtgact taaaattgta ttgataaaac agattgcctc cactgaatgt
    18901 ggaggttttt cttcgtgtat tggaagaata aattgctatc gaaaactata gtaaaatgaa
    18961 ataagaatag gacaaatcaa tcaggacagt caaatcgatt tctaacaatg ttttagaagt
    19021 agaggtgtac tattctagtt tcaatctact atataaagat atttttcggt caaatatctt
    19081 gctagggctt tcattttttg ctatactggt tcagtaatta aataatagag acatttgggg
    19141 tgctttaagc tgagatgata cccattgaac ctgatacagt taagactggc gaagggaaat
    19201 gtgaaacgtt ttttcgtatg gcgaaatgaa cgatctaatc ttgtaagtca actctaattc
    19261 ttgattgtaa ttggagtttt ttgtttatat aaactggata ggcttgttta gttagctctc
    19321 tgcaaatgct ctcgatgtgc tttaaaagga tcaagtggaa ttacgatttt gcttgatatt
    19381 tcttggtgtt ttattaatta ctaacaagta aagagaggag aagtaaatat ggaaacacaa
    19441 gactatgcat ttcagccagg tttgactgtt ggagaattat taaaaagcag tcagaaggat
    19501 tggcaggctg caatcaatca tcgttttgtt aaggaacttt ttgcggggac aattgagaat
    19561 aaggtcttaa aggactatct gattcaagat tatcacttct ttgatgcctt cttatccatg
    19621 ctgggtgcct gcgtagctca cgcagataaa cttgaatcca agcttcgttt tgccaagcaa
    19681 ctaggctttc ttgaagcaga tgaagatggt tatttccaaa aggcttttaa agagttaaaa
    19741 gtagcagaga atgactatct agaagtgact ttgcaccctg taacaaaagc ctttcaggat
    19801 ctaatgtatt cggcagtggc ttcatcagac tatgcccatc ttttggttat gctggtcatt
    19861 gcagaaggtc tctatttaga ctggggttct aaagatttgg ctctacctga agtctatatt
    19921 cattcggaat ggatcaatct ccacagaggt cctttctttg cagagtgggt tcaatttctg
    19981 gttgacgaac tcaatcgtgt cggtaaaaat cgagaagatt tgacagaact tcagcaacgc
    20041 tggaatcaag cagtcgcttt agaattagct ttttttgata ttggttacga tgtctagaga
    20101 aggctcatga tatttacaaa tttgatgagg taatactctt cgaaaatctc ttcaaaccac
    20161 gtcagtttca ccttgccgta ggtatggtta ctgacttcgt cagttctatc cacaacctca
    20221 aaacagtgtt ttgagctgac ttcgtcagtc ttatctacag gttcaaagca gtgttttgag
    20281 caatctgcgg ttagcttcct agtttgctct ttgattttca ttgagtataa atcattcaac
    20341 gtaaatgaaa aaaatttctt aacgaaagat agagataaat cgaaatcagt agatcgtata
    20401 aagtgaaaag gaaggatttc aatatgacaa gtttaaaatt attaaaaaaa aaagcaccat
    20461 tggtcatttg tataaccaat gatgtagtaa aaaatttcac agcaaatgga ttagtagcac
    20521 tgggcgcctc accagccatg agtgagtttc cagcagattt agaggatttg ttaaagtatg
    20581 ctggtggttt attaataaac ataggaacat tgacagatga aaattggaaa ttataccaag
    20641 ctgctctgaa aattgcggag aaatataatg tcccagcagt tttagatcct gtagcctgtg
    20701 gagcaggaga atatagaaaa aaagtagcag atgatctaat caacaattat aaactagcag
    20761 cgattagagg aaatgctggc gagattgcct ctttagtagg aatggatgtg gcatctaaag
    20821 gagtagatag tgcgggcgta gataatattg acgaaattgc tctagcagca aatgagaagt
    20881 tcaatattcc aatagtagta acaggtgaag tggatgccat tgctgttaat ggagaagtgg
    20941 taacgattca taatggtagt gctatgatgc cgaaagtcat tgggacagga tgcttattag
    21001 gagctgtagt agcaagcttt atcggacttg aaaaaggtca agaattgaaa tcattagaaa
    21061 cagcaatgtt ggtttacaat atcgctggag aaatggcgga aaaacgtcca aatggacatc
    21121 ttcctggaac atttaaagtt gaatttataa atgccttata tgaaattaca gatgaagatg
    21181 tcaagaaatt caaaagagtg aagtaagatg tttcataaag aattactaaa actatatttt
    21241 atttgtggaa cgactacttg ccaaggaaaa gatctatata cagtcgttga ggaagcctta
    21301 aaaggtggta taaccttatt tcaatttcga gaaaaaggtg agggtgcttt agaaggctta
    21361 gaaaaacttg agctagctat ccaaattaaa gaactttgta aaaaatacaa tgttccattt
    21421 atcgttaatg acgatataga tttagcaatg gaaattgacg ccgatggcgt acatgtaggt
    21481 caagatgata ttggtgttga tgaaattaga aaattgatgc cagataaaat aattggtctt
    21541 tctataaaaa acgaggaaga atttcaacaa tcaaaagttg aatatgtaga ttatgtaggt
    21601 gttgggcctg tatttgatac ccagtcaaaa gatgatgctg gtggtgctat aggttatgaa
    21661 ggtcttgaat tgatgagaaa actattgcca caaatgcctt tagttgcaat tggtgggata
    21721 cagacgaaac atattaaaga cattataaag accaatgttg acggcgtttc aatcatttcg
    21781 gcaatatcgt atgcaaaaaa tatagaaaaa actgttcggg aaatgagtga acaatagata
    21841 tctatccttg gattctaagt aagtaggatt ttcctttttt aaaaagctat gtctagattc
    21901 agcttgaatg gctaattcat caaacaggtc atttgttttt tgcaaaagaa agatctgctg
    21961 tctttgtgag tgcaaggctg tatttccagt tcaatttctt gaactagtat aaaatgtgtg
    22021 ttataataat tacataatta aataataaag gcatttgggg tgctttaggc tgagatgata
    22081 cccattgaac ctgatacagt taagactggc gaagggaaat gtgaaatgtt tttttcgtat
    22141 gtcgaaaaga actgtctaat cttgtaagct gaactctaat tcgtatctgt aattagagtt
    22201 ttttctttta cgaaactgga tgaagatg
//
LOCUS       DBERCA010000024        21926 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.024, whole genome shotgun sequence.
ACCESSION   DBERCA010000024 DBERCA010000000
VERSION     DBERCA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.024"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(226..1074)
                     /locus_tag="ACL719_001113"
     CDS             complement(226..1074)
                     /locus_tag="ACL719_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGS0699093.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1226..1504
                     /locus_tag="ACL719_001114"
     CDS             1226..1504
                     /locus_tag="ACL719_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGS0699094.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1506..3569
                     /locus_tag="ACL719_001115"
     CDS             1506..3569
                     /locus_tag="ACL719_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS0699095.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3647..4903)
                     /gene="tyrS"
                     /locus_tag="ACL719_001116"
     CDS             complement(3647..4903)
                     /gene="tyrS"
                     /locus_tag="ACL719_001116"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGS0699096.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5045..7510
                     /gene="pbp1b"
                     /locus_tag="ACL719_001117"
     CDS             5045..7510
                     /gene="pbp1b"
                     /locus_tag="ACL719_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGS0699097.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALVPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7575..8486
                     /locus_tag="ACL719_001118"
     CDS             7575..8486
                     /locus_tag="ACL719_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0699098.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8716..9414
                     /gene="dapD"
                     /locus_tag="ACL719_001119"
     CDS             8716..9414
                     /gene="dapD"
                     /locus_tag="ACL719_001119"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGS0699099.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9482..10612
                     /locus_tag="ACL719_001120"
     CDS             9482..10612
                     /locus_tag="ACL719_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGS0699100.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10624..11163
                     /locus_tag="ACL719_001121"
     CDS             10624..11163
                     /locus_tag="ACL719_001121"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGS0699101.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDLQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11147..11824
                     /locus_tag="ACL719_001122"
     CDS             11147..11824
                     /locus_tag="ACL719_001122"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGS0699102.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYMTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRLYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(11891..12790)
                     /gene="galU"
                     /locus_tag="ACL719_001123"
     CDS             complement(11891..12790)
                     /gene="galU"
                     /locus_tag="ACL719_001123"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGS0699103.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDEATGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMNDYEKTHAST
                     IAVMPVPHEDVSSYGVIAPQDEGKDGLYSVETFVEKPAPEETPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFARKFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12812..13828)
                     /locus_tag="ACL719_001124"
     CDS             complement(12812..13828)
                     /locus_tag="ACL719_001124"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS0699104.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKPYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVAVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14076..14180
                     /locus_tag="ACL719_001125"
                     /pseudo
     CDS             14076..14180
                     /locus_tag="ACL719_001125"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            14345..14479
                     /locus_tag="ACL719_001126"
     CDS             14345..14479
                     /locus_tag="ACL719_001126"
                     /inference="COORDINATES: protein motif:HMM:NF037495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699105.1"
                     /translation="MFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEILSFW
                     "
     gene            complement(14594..15025)
                     /locus_tag="ACL719_001127"
                     /pseudo
     CDS             complement(14594..15025)
                     /locus_tag="ACL719_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15087..15737)
                     /gene="phoU"
                     /locus_tag="ACL719_001128"
     CDS             complement(15087..15737)
                     /gene="phoU"
                     /locus_tag="ACL719_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS0699106.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15752..16504)
                     /gene="pstB"
                     /locus_tag="ACL719_001129"
     CDS             complement(15752..16504)
                     /gene="pstB"
                     /locus_tag="ACL719_001129"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0699107.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16506..17321)
                     /gene="pstA"
                     /locus_tag="ACL719_001130"
     CDS             complement(16506..17321)
                     /gene="pstA"
                     /locus_tag="ACL719_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS0699108.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17314..18177)
                     /gene="pstC"
                     /locus_tag="ACL719_001131"
     CDS             complement(17314..18177)
                     /gene="pstC"
                     /locus_tag="ACL719_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS0699109.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18295..19170)
                     /locus_tag="ACL719_001132"
     CDS             complement(18295..19170)
                     /locus_tag="ACL719_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS0699110.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19337..20668)
                     /gene="pnpS"
                     /locus_tag="ACL719_001133"
     CDS             complement(19337..20668)
                     /gene="pnpS"
                     /locus_tag="ACL719_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGS0699111.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20669..21376)
                     /locus_tag="ACL719_001134"
     CDS             complement(20669..21376)
                     /locus_tag="ACL719_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0699112.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21460..21926)
                     /locus_tag="ACL719_001135"
                     /pseudo
     CDS             complement(21460..21926)
                     /locus_tag="ACL719_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         6361 a         4189 c         4743 g         6633 t
ORIGIN      
        1 acgggagaaa tcgaaatact tgatttatac ggcctatatc atatttgggg taaatgtagt
       61 atatggtatt caatggttat taagagaact gatttcaact atttcccctt aatgtgtatg
      121 gaaactataa caaaacgcat aagattaagg tttgtgtacc tgttcttatg cgtttttata
      181 atattgaaga ttttcggtaa gttttatacc tttgggaagt ctagtttaga atgctttccc
      241 aaccaaaatc tctgcttcga tggtaatctc tgtcagttgg ctccagtcaa tcttgctctg
      301 gtcaacgtga aagaggagag gagtcatact gagtagggct tggagttgtt ctgctgtgat
      361 agtcttagtc agagaggcag tttgactaga taggatggta aagtgttcct ggaaatgctc
      421 cttgatatct tggttagaat actccttgtt tgtcagctgg tcctgtactc tttgacggat
      481 ttctttgagg tgattttcag ttgggataac ctttatcaag ataccgtctt tggataaaac
      541 gcgacgaaat tctccatagt tggcaggtga gaagatatca agcagaatat ccatgttagc
      601 gtcttttata ggaagtcgtg ccaagtcgcc aacaaaccaa ttgactgccc agttgggttc
      661 acttttagca gcgatttgga ctgaatcttt ggagatgtca aaggcataga aagttttttc
      721 agagtgactt tcttgtagtt tgcgagaata gaatccttca ccacaaccga tatccaaaat
      781 tgttgtggta gtttttgagc ttgcaagcaa gtcagataca gcatctaaga tagcttggta
      841 aaagccggct tctaggattt gttgacggtt ttgaaaattt tccttgttat agttagcaga
      901 ttgcttgatt tgaggtgcta gattgacata gccaaatttc gccaagtcaa aagaatgacg
      961 gttgcagcac ttgaaattag tctctaacag agtcagattt tcttgacaga taggacaggc
     1021 aaaggcagtc gcagaagcaa aacgctgaag tttgggcttg agatttgtat tcatagttta
     1081 agtgtatcaa aagaggcaaa tgaaagcaac tttaggaata agtagatggg agattcagta
     1141 gaaataaaac taattctcca atttatagaa tgaaattgct tttatatcta aattgctata
     1201 taataatgat aaggaggaaa taagtatgtt aaaagaagta ttaaccgttg caaaagttgc
     1261 gaaaaaatct tcattatttt tgggtggtgt cgcatttggt acccttggtt tgaaaatctt
     1321 agcaagtaag gaagctaaaa aaggttattc taaagctttg gctaaggctt acaagttgaa
     1381 agacgagcta gatgcatctg tttctgttgt gaagcaacat ggagacgatg tcttgcaaga
     1441 tgccaaatat ttgtacgagc aagagaaaaa agaagagcaa ttagatagcc ttataggtga
     1501 ataatatgtc ttttaaagtg ctacatagag gataccaaca tatccgacta tcatcttctt
     1561 tttcacttac cttggatatt caagactatc ttcgttcctt ggcgagagat gaaaagggga
     1621 ttgagtctat ccagttttac atggatcaac agcactttac tctacgcata aaagaaggct
     1681 tttctgtatt agataatgca gaagcctttt taaaaagaat tgataaaggg aaagtttctg
     1741 agttgatgac tcttcccatt cgtagagaag agagtgctta ttctattgtt tcaggtgcag
     1801 cggttaagcg tgtacttttt cgtagttttg tgccgtatcc tattcgctat atatggactt
     1861 gttatcaggc tttgggttat attagagaag cctatcaaac actagcgcgt aaggaactaa
     1921 ctatggaagt cttggactgt tcggctattt tattgtcttt gtttatgaac caatccaaga
     1981 cagctagcaa tatcatgttt atgcttgatt tggggaatca tttagatcag tggtccttga
     2041 aaaaaactgc aacagattta gaacagagtc ttcttgcaaa agagagcgat gtattcctag
     2101 tacagggcga tacggttgtt agtataaaga gttccgatgt tcaaatagga gatgtcttga
     2161 tcttatctca aggaaatgaa attctgtttg atgggcaagt agtttcaggt ttaggtatgg
     2221 tcaacgaaag ttccttgaca ggagagagtt ttccagttga aaaaagagag tctgatttgg
     2281 tttgtgcaaa tacagtatta gaaactggag agttacgcat tcgtgtaaca gataatcaga
     2341 tgaacagccg tattttacag ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa
     2401 cgaaacaacg ctatttcatc aagatggcgg ataaagtcgt caaatataat ttcttggggg
     2461 ctgggctgac ttacctattg acaggttctt tttctaaggc tatttctttc ctattggtcg
     2521 atttctcctg cgctttgaaa atctctactc ctgtagctta tttgacagtt atcaaggagg
     2581 ggttgaaccg tgaaatggtg attaaggatg gagatgttct ggagaaatat ctggaagttg
     2641 atactttctt gtttgataag acaggaacaa tcacaactag ttatcctata gttgaaaagg
     2701 tgttaccttt tggagactat agtgaggaag atattctcag aatcagtgcc tgtcttgagg
     2761 aacacattta tcatcctatt gctaatgcca tcgtcaagca agctgagata gagggaattg
     2821 aacatgagga aatgcatggg aaactccaat atatcgcaag caaggggatc aaatctcata
     2881 tagatggcca accagttctt attgggaatt atgtcttgat gcaggatgag cagattcata
     2941 tcagttcaga acaaaatgct ttaattgaag agtacaagag tcactacaat ctcttattct
     3001 tggcttatca aaatgaattg attggaatgt tctgcattca tactcctttg agaaaagaag
     3061 caaaaacagc cttggataaa cttaaggcac aggggaaaaa attgattctg gcaacaggag
     3121 acaccttgat taggacagag gaattagtca aagatttgcc ctttgatcag gtctatacag
     3181 acttgaaacc tgatgggaaa tttgagttag tagagaaact gcagaaagca ggtcacacta
     3241 ttttgatggt tggagatgga ttgaatgact cagcggcttt aactctatca gatatcggtg
     3301 tggtgatgaa tgagagtgca gatatttcta agcagatgag tgatatctta ttgttagata
     3361 atcgtttgga tttcttccaa gagttggatt cgttatcatc atctttgcaa acactcatca
     3421 agaaaaatat tcaagatacc gttgtcgtaa atagtagttt gattggcttt ggcttattta
     3481 attggttcag tccttcaaat ctctctatct tacataatct aacaaccttg cgcattgtct
     3541 tgcgtagctt gtctattaaa aatagataga tgagagttat taacagcaaa aaggagttac
     3601 ctttctcgag gttaactcct ttgtttatag ataaatgttg aatagtttag taagtcaata
     3661 caaagtattt tttcttccca cgacggataa cagtcagttc attttctaac ttatcagcgt
     3721 cactcaagac atagtcaagg tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt
     3781 ggacgtcttc acgggcttgg cgttttgagt taactatacc agatgagacg agcagttcca
     3841 cgatattgtt atgttcgtct gcctgtactt ggtagtttgg cacaccacga agtccttgtt
     3901 tgagctcttt gacagaaagg tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa
     3961 gtgcttcttt gtaggcttct tctccgtgaa caagtgtaac aacttcacga gccaagactt
     4021 tttgagccaa gcgttcgtgt ggcgctgctt caaattgttt acgaatatct tcaatctcat
     4081 caagtgacaa gaaagtaaag attttcaaga agcgaacagc gtcagcgtcc atcacgttca
     4141 tccagaattg gtacatttcg tatggagaag tcttttcggg attgagccag acggcatttc
     4201 cttctgattt accaaatttc ttaccagttg catctgtgat tagtggaaca gtgataacgt
     4261 gaccagtctt gtccgcctta cgacgaagca attcggtacc agctgtcata tttccccact
     4321 ggtcagaacc accgatttga agagtgacat tatggtcttg gttaaggacg aagaagtcgt
     4381 acccttgcat gatttggtaa gcgaactcag tgtaagaaat tcctgtttcg atccgttttt
     4441 taacagattc cttactcatc atgtagttga ccgtgaagta ttttccaata tcacggagga
     4501 agtcaatgaa gctgatgctg ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt
     4561 cgccattttc aaagtcaaga aaacgagaaa gttgtccttg gatagacttg acccagccat
     4621 ctactgtgtc ttttgtttgg agactacgtt cagcatcttt gaaggacgga tctccgatga
     4681 gacctgtagc accgccaacg agcgcataag gtttgtgacc tgctagttgc aagcgacgac
     4741 ttgtcaagat tgcgacaagg tggcctaggt gaaggctgtc agcagttgga tcgtagccag
     4801 tataataaga aacttgacct tcttctaggg ctttacgcaa agcttcttca tcagtcgttt
     4861 gaaatatcaa accacgctct tttagctcat caaaaatgtg catgtgtctt ttctcctttt
     4921 taaaattatt gtttctacct attgtatcac aaacttgggc aatagcctag cagaataggg
     4981 aagaaagtgg ctattttatt gaaaatttcc cttttgtggt ataatagata gagtgaggaa
     5041 atccatgcaa aatcaattaa atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa
     5101 acaaaaaaat aaaaaatcag ctagacctgg caagaaaggt tcaagtacca aaaaatctaa
     5161 aaccttagat aagtcagcca ttttcccagc tattttactg agtataaaag ccttatttaa
     5221 cttactcttt gtactcggtt ttctaggagg aatgttggga gctgggattg ctttggggta
     5281 cggagtggcc ttatttgaca aggttcgggt gcctcagaca gaagaattgg tgaatcaggt
     5341 caaggacatc tcttctattt cagagattac ctattcggac gggacggtga ttgcttccat
     5401 agagagtgat ttgttgcgca cttctatctc atctgagcaa atttcggaaa atctgaagaa
     5461 ggctatcatt gcgacagaag atgaacactt taaagaacat aagggtgtag tacccaaggc
     5521 ggtgattcgt gcgaccttgg ggaaatttgt aggtttgggt tcctctagtg ggggttcaac
     5581 cttgacccag caactaatta aacagcaggt ggttggggat gcgccgacct tggctcgtaa
     5641 ggcggcagag attgtggatg ctcttgcctt ggaacgcgcc atgaataaag atgagatttt
     5701 aacgacctat ctcaatgtgg ctccctttgg ccgaaataat aagggacaga atattgcagg
     5761 ggctcggcaa gcagctgagg gaattttcgg tgtagatgcc agtcagttga ctgttcctca
     5821 agcagcattt ttagcaggac ttccacagag tcccattact tactctcctt atgaaaatac
     5881 tggggagttg aagagtgatg aagacctaga aattggctta agacgggcta aggcagttct
     5941 ttacagtatg tatcgtacag gtgcattaag caaagacgag tattctcagt acaaggatta
     6001 tgaccttaaa caggactttt taccatcggg cactgttaca ggaatttcac gagactattt
     6061 atactttaca actttggcag aagctcaaga acgtatgtat gactatctag ctcagagaga
     6121 caatgtctcc gctaaggagt tgaaaaatga ggcaactcag aagttttatc gagatttggc
     6181 agccaaggaa attgaaaatg gtggttataa gattactact accatagatc agaaaattca
     6241 ttctgccatg caaagtgcgg ttgctgatta tggctatctt ttagacgatg gaacaggtcg
     6301 tgtagaagta gggaatgtct tgatggacaa ccaaacaggt gctattctag gctttgtagg
     6361 tggtcgtaat tatcaagaaa atcaaaataa tcatgccttt gataccaaac gttcgccagc
     6421 ttctactacc aagcccttgc tggcctacgg tattgctatt gatcagggct tgatgggaag
     6481 tgaaacgatt ctatctaact atccaacaaa ctttgctaat ggcaatccga ttatgtatgc
     6541 taatagcaag ggaacaggaa tgatgacctt gggagaagct ctgaactatt catggaatat
     6601 ccctgcttac tggacctatc gtatgctccg tgaaaagggt gttgatgtca agggttatat
     6661 ggaaaagatg ggttacgaga ttcctgagta cggtattgag agcttgccaa tgggtggtgg
     6721 tattgaagtc acagttgccc agcataccaa tggctatcag accttagcta ataatggagt
     6781 ttatcatcag aagcatgtga tttcaaagat tgaagcagca gatggtagag tggtgtatga
     6841 gtatcaggat aaaccggttc aagtctattc aaaagctact gcgacgatta tgcagggatt
     6901 gctacgagaa gttctatcct ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt
     6961 aaatcctact ctggctaatg cagattggat tgggaagact ggtacaacca accaagacga
     7021 aaatatgtgg ctcatgcttt cgacacctag attaacccta ggtggctgga ttgggcatga
     7081 tgataatcat tcattgtcac gtagagcagg ttattctaat aactctaatt acatggctca
     7141 tctagtaaat gcgattcagc aagcttcccc aagcatttgg gggaacgagc gctttgcttt
     7201 agttcctagt gtagtgaaat cggaagtctt gaaatcaaca ggtcaaaaac cagggaaggt
     7261 ttctgttgaa ggaaaagaag tagaggtcac aggttcgact gttaccagct attgggctaa
     7321 taagtcagga gcgccagcga caagttatcg ctttgctatt ggcggaagtg atgcggatta
     7381 tcagaatgct tggtctagta ttgtggggag tctaccaact ccatccagct ccagcagttc
     7441 aagtagtagt tctagcgata gcagtaactc aagtactaca cgaccttctt cttcaagggc
     7501 gagacgataa ttctctaaat gaagtggcca atcaatggat tgccactttt ttcctttcgt
     7561 gttacaataa gggtatgaat cagtatcaga aaaagattgt taacggaaaa atttattcgc
     7621 tcctatccgg cttaatatgg ggaatctgtg gaattttagg agagtacttc tttactcatt
     7681 atcaggtgtc ttcgggctgg attacctcta tgcgtttgac actggcaggg agtcttgtac
     7741 tcatttggtc tgcaatacaa ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa
     7801 attacctgcc ctttttagcc tatgctattt tggggatttt ttcagttcag tattttttct
     7861 atctctgtgt agaatactca aatgctacga cagcaactat tttacagttt attagccctg
     7921 tctttatcct cttttacaat cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt
     7981 tctatgtttt ggttgccatg ctgggtgttt gcttgatggc gacaaaggga gacctctctc
     8041 agttatccat gacgccgcta gctcttataa caggtttgct gagtgccatg ggtgttatgt
     8101 ttaatgttat cttgccccaa ccttttgcta agcgttatgg ttttgttcct acggttgggt
     8161 gggggatgat tttggcaggt ttgtttagca atgtcctctc gccggtttat cagctttcct
     8221 ttactcttga tatttggagt atcttgattt gcctcattat cgctttcttt ggaacggctt
     8281 ttgctttttt catttccatg aaggctgtgt ccttggtttc tcctttggtg gtttccgtta
     8341 tcagtgccag tgaacctctc tcttctgctc tcttgagtgt tttgttctta ggattagtag
     8401 tggattggtc cctccttcta gctatagcct tgattatttt acccatgata tttttgtcta
     8461 tagaagaagc gaaagaaagt agataaaaag tcttttctta attctaaaag tgtgctatac
     8521 tagaatagtc aataaaacac ggggagatag ttgtgaagag tgtttttagg ttgtatcggt
     8581 aggggcttgc ttttaccgac agcacgtttt atctcacatc cctaaaaaat catacctaaa
     8641 aacagacaaa aaggtttcaa atttgaggcc ttttttggta gaataggtat cattataacg
     8701 aaccaggagg cacctatgac tgctacaaaa atgaacgctc aagaaattat ccaatttatc
     8761 gccaatgctg aaaagaaaac cagtgtcaaa gtaacctttg aggggcaact cgcaactgct
     8821 gtgcctagct ctgttgtcaa actaggaaat gtcctattcg gagactggaa ggacgtggct
     8881 ccgcttcttg aaggtttggt agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat
     8941 tctgcagttc ctttgctaga taagcgtgct atcaacgctc gtatcgagcc aggtgctatt
     9001 atccgtgacc aggtggaaat tggtgacaat gctgttatca tgatgggagc tgttatcaat
     9061 atcggtgctg aaatcggtgc tggaaccatg attgacatgg gtgccatcct tggtggccgt
     9121 gccatcgttg gaaaaaatag ccacgttggt gcaggtgcag ttttggcagg tgtgattgag
     9181 ccagctagtg ctgaaccagt ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg
     9241 gttatcgaag gagtccaaat cggtagtggt tcagttgtcg cagcaggagc tattgttacc
     9301 caagatgtcc cagaaaacgt ggtagtagca ggtgttccag ctcgtatcat caaagaaatt
     9361 gatgcccaaa ctcaacaaaa aacagcgcta gaggatgcgc ttcgtacctt gtaattgtaa
     9421 aagtaaaaaa gaggcggaac cctttttcca gcctctttct gctatatcgg aggacagata
     9481 gatgttagat ttgattcaga ctagacgaga tttacaccag ataccagaga ttggcttgga
     9541 ggagttcaag actcaggctt atttgctgga tgtgattgag aaattgacta cgggcaagga
     9601 ttttgttcaa attcgtactt ggcggacagg gattttggtc tacctgcagg gaagtcagcc
     9661 ggagcgaacc attggttggc ggacagatat tgatggcctg cctatcgtcg aacaaacagg
     9721 tctacctttt gcttctcaac accaaggtcg catgcatgcc tgtggccatg attttcatat
     9781 gaccattgcc ttaggctgtc ttgagcgcgc ccttgaggag caacctaaaa ataatctgct
     9841 ctttctattt cagcctgctg aagaaaatga agctggtggt atgctcatgt atgaggctgg
     9901 tgcttttgga gattggttgc caaaccaatt ctatggtctc catgttcgtc cagatttgaa
     9961 agtcggccag attgcgacta atactcatac actctttgca ggaacttgcg aggtgaagat
    10021 ccgtttcaaa ggcaaaggtg gccacgcagc ttttcctcat gaagccaatg acgccttggt
    10081 ggcggctagt tactttgtga cccaagtgca gtcagttgtt agccgcaatg tcaacccaat
    10141 cgagggagca gtggtgacct tcggcgtttt tcaagctgga accaccaaca atgtcatcac
    10201 agacacagct tttttacatg gaactattcg tgccttgaca caggacatga gtctcttggt
    10261 gcaaaagagg gtaaaaacag ttgcagaagg ggttgcagct gcctttgata tggaagtcga
    10321 agtggaactc aaacaaggtg gatacctacc tgttgagaac aatccagcct tggcgcgtga
    10381 actgatggac ttctttgacg agaaagacgg aatcgagttg attgatatcg aacctgctat
    10441 gacaggcgag gactttggtt atctcctttc aaaggttgat ggcgtgatgt tttggctagg
    10501 tatcgatagt ccctacgctc ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc
    10561 tattggggtg gctgcggtgt ctagtttcct gaaaaagaag gcagcagaat agagggcttg
    10621 tctatgaaat cggaattacg caagcaagtc ttgcatgaaa tgaaggcttt atctcaagaa
    10681 caaaaacagg ctatagacca agctttaacc gagcgaattt tacaacaccc cttttatcaa
    10741 gaagccaagg tcatcgcaac ctacctctct ttttctcatg agtttcaaac gcgggaactg
    10801 attgagcagg cgctgaagga cggcaagaag gttttaatac ccaaaactta tcccaagggg
    10861 cgcatggact ttgtagtcta tgatctgcag cagttggtaa aaactgcctt tgccttactg
    10921 gagccacagg gagatttgga agtggtggat gtatctcaga ttgatttgat tcatgttcct
    10981 ggcctggctt ttacgacgga tggatatcgg attggatatg gtggaggtta ttatgaccgc
    11041 tatctggaac atttttctgg tcatactttg agtacggttc atccttgtca aattcaggac
    11101 tttatacctg aaaaccatga tattcctgtt caggaggtat taattgatga aggaaatctt
    11161 tgatagacgt taccctgtga cgagtttctt cctcttagtg acggccttgg tatttttact
    11221 aatgttggtc actgcaggcg gaaactttga cagggcagat acattatttc gatttggagc
    11281 catgtatggg ccagctattc gcctctttcc cgagcaggtt tggcgtctct tgtctgccat
    11341 ttttgttcat attgggtggg aacatttcat tgttaatatg ctttcacttt attatcttgg
    11401 aaggcaggta gaggagattt tcggctctaa gcagttcttt tttctctatc tcttatcagg
    11461 aatgatgggc aatctctttg tttttgtatt tagtcctaaa tccttagcag caggcgcctc
    11521 tacctctctt tatgggctat ttgccgcgat tatcattctt cgctatgcaa cgcgtaatcc
    11581 ttatatccag cagttgggcc aatcctatat gacacttttt gtggttaaca ttattggaag
    11641 tgttctgatt ccagggatca gcctagcagg ccatatcggt ggagcagttg gtggcgcatt
    11701 tctagcagtt atctttccag ttagaggaga aaaacggctg tataacacca gccagagatt
    11761 aggagcggta gtcttgttcg taggactcgc cattttgctt ttctacaagg gaatgggaat
    11821 gtgacgaagt tgaaagaaaa tttgtgtaat gaaaaaagat aaggcatttt ttgaacctta
    11881 tctttttatt ttattccttc tcagtcaatt cttttccaag ttggatgagg taattcttca
    11941 aatcatcttt gacttgtgga tgtttgaggg cgtagtcaat agatgttttc ataaagccaa
    12001 acttatcccc aacgtcgtaa cgagctcctg taaatttacg ggcaaaaaca cgctgtgttt
    12061 tattaagagt atcgattgca tcagtaagtt ggatttcatt tccagcacca ggagcttgct
    12121 tttcgagaat ctcaaaaatt tctggtgtga gtagatagcg accgatgatt gccaaatcac
    12181 ttggagtctc ctcgggagct ggtttttcaa caaaggtttc aacactgtaa agaccatctt
    12241 ttccttcgtc ttgaggagca atgactccat atgatgaaac gtcttcgtgt ggtacgggca
    12301 tgacagcaat ggttgatgcg tgtgtctttt cgtaatcatt catcagttgt tttgtcaaag
    12361 gaacggcctt ctcgtctgtg atgtccatca agtcatcacc cagcataaca acgaagggtt
    12421 catttcctac aaaagctttg gcttgtaaga cagcgtctcc aagaccacga gggtgagttt
    12481 ggcggatgaa atgaaggcgc atgccagttg cttcatctac caattttaac aaatccgttt
    12541 tgcctttttc cttaagattg tattcaagct caaagtttga atcaaagtgg tcctcaatag
    12601 aacgttttga tttaccagtg acaactagaa tatcttcaat acctgatttg agagcctctt
    12661 ccacgataaa ctggatagtt ggtttgtcta cgattggcaa catttctttg gcaagggcct
    12721 tggttgctgg taaaaatcga gttcctagtc cagcagcagg gataactgcc tttctaactt
    12781 ttgatgtcat aaaaatcctt tctatagagg gttaagacca ctcattttct gctttaaatt
    12841 cattgttcat gatgtcataa atggcatctt tgatattggt tccgtgataa ataacttggt
    12901 aaatagcctg tgtaatgggc atatagactc caagttcttg ggctagttca taggctgctc
    12961 gagtcgttga aattccttcg attaccatgc ccatattagc ttctatatca gctagggatt
    13021 ctcctcgtcc gagagcatct ccagctctcc agttacgaga gtggatggaa gttcccgtta
    13081 cgatcaaatc tcccacacca gataagccgc tataggtcaa tggactggcc ccgagtgcta
    13141 cccctaggcg ggtgatttct gctaaacctc gagcgatgat ggctgcctta gcattatcac
    13201 caaatccaag accatgtaaa gctccagcac cgacagcaat aatattttta agagcaccag
    13261 cagtttcaac cccgataaca tccgtattgg tataaagtcg gaagtagtga ttactaaata
    13321 gctcctgaac gtattgagct gtttgtaaat ctttagaagc agcagttatt aaagttaggt
    13381 cacgcacaat ggtctcttct gcatgactag gccctgaaac agcgacgata tcactacgga
    13441 gatgttcagg aatttcttct tcaagaatgg ttgataatcg tttatggcta tcaggttcta
    13501 atccctttga tgcgtgcatg atgataacct tatggtccaa ggtttgtgca acttgctggg
    13561 caacaagtcg tgtcactttt gttgggacaa caaacaaaat cgcatccaca tttttcaatg
    13621 tttctgctaa gtcggtgtag gcaatgatat tttcgtctag aacgacatct ttaaagtagg
    13681 gcttattagt atggtgtgta ttaatttcat tgatttgctc gggaagattt ccccaaatac
    13741 gtacctcgtg tccattgtca tttaagactt gtgaaagggc ggttccccaa gaaccaggcc
    13801 ccaagacggc gacggtttgt ttttccatga tttcctcctt gatagatttc tttctcttat
    13861 tatatcatat tctaggggat tttgcacttg cattactggg gtatggtgga aaaatacaca
    13921 aagatcgaga tgaagggata aactcttagt ctttagttaa agtgagaaga aatctgatag
    13981 atagtttgat tcagtatatg ctataaatag aggtaaacaa ctcaggaggt tcttgtaggt
    14041 tgcgaaagtt tggcgaaaaa attaaaagat tacgtttggc taaaaaaatc agtcgttcag
    14101 aattttgtgg tgatgagtct gaattaagta tccgtcaatt aattagaatt gaaaatggag
    14161 aatccagacc aacactaaca aagttaaaat atatcgctga acgtttgggg gttgaagatt
    14221 acaagttgat gccaagttat atagagttgg ataaggaata cctagaattg aagtatttct
    14281 tgatgaggac tcctacatac gaagatgaaa ctatcgccca aaagaaagag agtgttttga
    14341 taagattttt gaagagtatt atgataggct acctgaggaa gaaagattta tcatcccaaa
    14401 ttattcatat ctggcactaa cgaactacac agttcaaaaa ttaccagaaa agctagttga
    14461 aatactgtcc ttctggtgat tttgcttaat ttctgatgtt cgagacataa ttgaggagat
    14521 ttgtcttgat tttttcctaa aaaagggcag actcctccct tggtttgatg ctcgattttt
    14581 aaaataaggt gacttattct gttaggcatg ttttcccctg ccctctagct ctatatggaa
    14641 cagggagtat cagatttatc tggcagattg tatcaaataa ggcttgaaac tgttttgaag
    14701 aggcatatag acttgagaat ttgagaatat gtcgtctagc agtagagaca tattttccgg
    14761 caatatgaag gaagagacgt cgaaaacgct tgatagtcaa ggcctttact tcatcaccag
    14821 ctagctgctt taaaaagagg tagagattat aagccagaca gcccatcatc atacgtactt
    14881 cattcttaat catggtcgaa ctatctgtct tatccccaaa gaatcctgct ttcctttctt
    14941 tgataaagtt ttcagcattc cctcgttcac gatagaattg aaacagctcc tcactatcta
    15001 cactgcttaa attgttcaaa tgaacttttt tacaaccata aaaaagagca cttaatcgta
    15061 ctcttctctt ttaaggatta gttgaattaa ttcaaatcca ctagttctcc tgtttctagg
    15121 tagactaggc gttcacaaat gttagcaatg taatcagcga agcgttccag atgccctatg
    15181 atataaaggt attgtgttcc attgggaatt gaggtttctt ggtctttcat aagtccaatg
    15241 atttcctttg ataaggcata ataatattgg tcaatctgtt catctttttg agcaatacta
    15301 atagcttttg aggcttggtg caaaggaaag gcaaccaata aatcggctag catgctgagg
    15361 gataatttac ccatttggtg taactgttct tcgtcagggg ctagttgatt ttcttttagt
    15421 tgcaaaacag ctttggcaat gcctgccata tggtctccca tacgttcaag gtctgaacaa
    15481 gaagacatga tgctaatcac aaatcgaagg tcagacactt gtggctgctg caaggccaac
    15541 aaacgggcac aggtcaattc gatagcgctt tgaccttggt tgatagcatg atccttattg
    15601 ataattagct ctgccatctc cttgtctttg gaggctaagg ccagtaaggc ttttgaagct
    15661 gtttcaagga caagttgccc tagtcctaaa aaggattgtt ctaattcatg caattctaag
    15721 tcaaattgat ttctcatagg tttttccttc cttatccgaa ccgtcctgaa atatagtctt
    15781 ctgtgcgctg atcttttgga ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt
    15841 ctcctgttaa gaaaaaagca gttttatctg aaatacgtga agcttgttgc atgttatggg
    15901 taacaatgat aatcgtataa tcttttttta gttgctgaat gaggtcttca atttttaaag
    15961 tggagatagg gtctaaggct gaagtcggct catccattaa cagaatatca ggttctactg
    16021 ctaaagctcg cgcaatgcaa aggcgttgct gctgaccgcc agataaggac atggcactct
    16081 ttttaagatc atctttgact tcttcccaaa tggctgcccc ttttaaagat ttctccacta
    16141 aggcatctaa ttgttttttg tctcgaatac catgtgtcct tgggccataa gccacgttat
    16201 catagataga catggcaaag ggattaggct gttgaaaaac catccctaca cgcttacgta
    16261 gctgattaag gttgaatttg ctactataaa tatcttgctc atctaagagg acttggcctt
    16321 caatatggca agaaggaacc aaatcgttca tccggttaag ggtttttaga aaagttgatt
    16381 tgccacaacc agatgggcct atcaaggcag taatctgcct ttctggtaat tgaatcgaaa
    16441 tattttttaa ggcttgaaaa tccccgtaaa ataagtctag gtgtctaact gaaaatgttc
    16501 ccatactagg aagctccttt cacaagtttt cgagataata agcttgatag agtatttatc
    16561 attaaaacag taataatcaa aatcacaccg gtagcatagg cttcattgac atgtagcccc
    16621 tcacttgaca gcatatacat atgcagggct agagaacgac ctgaagacat gagactactt
    16681 ggcgtattgg tagaggtacc taatgtatac atgagggcag ctgtctcacc aacgatacgg
    16741 ccaatagcta gtatcactcc agctaaaata cctggcatgg caactggtag aacaattcta
    16801 aaaacagtcc gtaacttacc tgccccaagt ccataacttg cttgacgcat gctatcacta
    16861 acagataaaa gggcttcttc tgttgagcga ataatgactg gcaacaccat gatgactgag
    16921 gttaagattc ctgataacag agagtattga aaacctaaga agactacaaa gaagagcatg
    16981 ccaaacagac caaaaacaat ggaaggaatc ccagacaagg tatctgaggc caatcgcatg
    17041 attttaacac aaagggaatc tttttttgta tattccacaa gataaaaacc agcaaaaatc
    17101 cctatgggca aggctaaaag aagagcacca aagaccagaa taacggtgga aataatcgct
    17161 ggcataaggg aaatgttctc agaagtataa gtccaagaaa agagggatag acttagatga
    17221 ggtaagcctt tgatgaggat aaaaccaatg attaaaaaga gagagccaaa ggttaaagct
    17281 gaaaaacaat aaacgagaag ttttagcagg tatttactca taagatgatt ttcctttcaa
    17341 gtaggcaaag taggcattaa tcaagagaat aaggaaaaag agaactgctg aggttgcaat
    17401 aagggcttcc ctatgctgac ctgatgcgta agccatttcc agaacaatat tggttgttaa
    17461 ggttctggtt cctgaaaaga gtccacttgg aataatcggc tggttgcctg ccaccaagat
    17521 aactgccatg gtttcaccta ctgcgcgacc gattcctaaa ataactgctg ataaaatacc
    17581 agatctcgca gctggcaaga tgacactaaa aatactccgt tcatgactag ctcctagagc
    17641 caagctacca gaataatacg ttttgggaac tgttcggata gcagattctg acaaactgat
    17701 aatagttggc aaaatcatta ttcctaatag taacgaagcg gttaggacac tcatgccatt
    17761 tcctaaaaag cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac cataaacaat
    17821 agatggaatg gctgccatca agttgatagc tgattttaag aagccataga cgggctttgg
    17881 acaataataa accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc
    17941 tcctaaggta attaataagg aaccaacgat cattggtaaa ataccatagc ttgccggaat
    18001 gttcgttggc gaccaatcac tgcctaataa aaaacgggca aagccgtagt tagctatgaa
    18061 aggtaagcca ttactaaaaa taaagaaaca gattagcaaa atagctacaa cagctactgt
    18121 tgcactcatg aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt ttgtcacatc
    18181 ttgtcctttc tagtgaagaa agtaagggag atacgacacc tccctacttg ccttctttat
    18241 cttattgtac gatgaaacgt ctgcatctct ttagagattt atggagcaaa cattttattt
    18301 aatcttgtcc caggtggtta atttgccact aaaaacgtct gcaagttcag ccatactgac
    18361 ttggcttgcc ttattgtcat tattgaccac aacagcaata ccgtctaaag caatagcatc
    18421 atgggtgaga ctcttacctt cttcaggagt taattcccta gaaaccatac caatatcagc
    18481 ggttttctcc ttaacagcgg taatacctgc tgaagaccca ttagaggtaa tatcaatcgt
    18541 aacttctgga ttttcttttt tataagcttc tgctaatttt tccattaaag aagatactga
    18601 agtggaacct acaacagaca acttgcctga taagtgttgg cttgtatatt ctgtggtttc
    18661 agttttagct tcaataaatt tattatctgt gaccacttgt tgaccttgtt tggagtggat
    18721 aaagctgata aaatcttgac ctagcttgga aagattagaa gaccaaacaa tgttgaaggg
    18781 acgttgaaga gggtattcac catctaaaac tgtgtctcga ctagccttga caccatcaat
    18841 ctctaaagcc ttgacagatt tcgttaaaga tcccaaggag atgtagccga tagcattagc
    18901 attcccttga actgctgaga gaacaccttc tgtactattt tgaatcacag ctgttttggc
    18961 agtgttgtca atttttttat caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc
    19021 accccgtgtt ccagagccat tttctcgtga aatcacctca atcgttcctg aagctgactg
    19081 tttggaagca gctgactgat tgccacaggc aacaagccca aatcctgata agccaatggc
    19141 tgcaagagta agcatttttt tgaatttcat aataatcacc tttatctcta tgtatttttc
    19201 ttgtgtaggc ttactacatt tatagtctaa caagtctttg taaaggttta tccctgattc
    19261 atgtaaagat tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa
    19321 atagctttaa aaattattag tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc
    19381 aactgcctct gccaagctga ctcgtcaccg tgacttggcc acctaataat tgactgagtt
    19441 ctttgacaat ggcaaggcca agaccagtgc caccagtttg tctgcttcga cctttattaa
    19501 ctcggtaaaa acgttcaaaa atacgatcct gctctaattg actaatacca atccctgtat
    19561 ctgatacaga aatcttaatg ccttcgttca ccttttgggt cttgacctca atttttcccc
    19621 cttgttcagt gtaacggatg gcattggata aaagattgag taagatttgg gaaagtaatt
    19681 gactatctga tacgagggtg acatcatctg gcacctgcac ctttagctgt aaatccttct
    19741 tcttgagctg aggttgcaag ctttgagtca aatcctgtac aaattctgcc aaagaaaggg
    19801 tcgtccattg tataggcatt tgttgagcct tagataaggt aagaagatgc tcaacaatat
    19861 gctcaagacg caaactttct ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt
    19921 cttcagctga catcccctta atggtttcag caaagccctt aatcgaagta actggtgtcc
    19981 tcaattcatg ggaggcattt gagacaaagg ctaaatttaa cttttcataa gttctaatcg
    20041 ttgttaaatc atatagcaag acgagcacag cttccacaga ttgggtgggg ctaaaaacgg
    20101 gaactgctgt tacttctaaa atcaagtcac cctcatgaaa cccacttact tcttgtttta
    20161 accttgtttt ttgatcaaag gcttggtgaa ctaaatttcg aatatccatc cgtttgaggt
    20221 catcaagtga acttatgtcg ccgtccacat cgggaaaata atgaggcaga gagcgactgg
    20281 ataataacat ctgaccttga gcggaaacta aaaacgtccc catggttagg tgcgacagaa
    20341 gaacctccat tgtttcggct aggtccttgt attgctgatc ctgttgggag actttggttt
    20401 ttaggccaga cacatactga gccaaagact ttaagtcttc ttgccctttt tctaaaaagt
    20461 attcactact ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc catttccaaa
    20521 ggcaaaagag ccagtagcca ccttgtccca aagaaagggc tagaagaaag agaccgatgc
    20581 ctttactgat ccaagttaat gccatccctg caatcagaat gaggctaaca cttagattga
    20641 ctagccaaaa ttgaaggtag cgtttcatct ataactcctt gaacttataa ccataacccc
    20701 gaatggttcg aataaattga ggggctttag gattgtcttc aattttttcc ctcaacttac
    20761 caatatgaac gtccaccaaa cgtgtttcct gcccaaagtc atacccccag atacgttcca
    20821 aaagacgctc tctagtcagt gtcatgttgg gatgtttcat aagatagagc aagagttcaa
    20881 attcttttgg ggtcaaactc agtaacttat tcgccttgta gacttcatga cgctcagggt
    20941 atactttcaa ggtcccaaat agccaagaat cgtcagcgat attatctgaa tcatctcctt
    21001 cttgttctcc tttagttcgc ctgaggacag ccttgacacg cgccagcaat tctctagggc
    21061 taaaaggctt ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt
    21121 catcactttt cgcagaaacc atcataattg gagttttgac gcctttggct ctcagccgct
    21181 tacaaacttc catgccatct aattgtggta acatgatatc aagcaagata aaatcaaagg
    21241 gttctgtttc tgccaaagct aaggccttcc gtccatttgt taccaattga gtagaaaagc
    21301 cttccttact taaatggtag tcaagcaatt tcagaatgtg ttcttcatca tccactaata
    21361 agacttgttt tgtcatctat tatctcctat tggtaacatt ataacacaat tatcagaaat
    21421 cctaacattg ctaaatcaga ttaaatttgc ttatcaagac tagtatctgg tcaaacgctc
    21481 aatcatctcc ttgtgctctg gataggtcgc cagtagatct accctttcaa ataattcaaa
    21541 atcctcaaat tcaaaaccag gagcaacaag acaagaaacc agagcatcat ccttatcaac
    21601 tgttgatccc caaatagtgc ccttaggaac acagtagtga agttgttgcc ctttggatat
    21661 gtccaggcct aaagtgactg cttcgtagtg accatctgct gtgatcatgt gaacagtaag
    21721 tggggatcct gcatgaaaat accagatttc atctgctgtc aatcggtgaa aatgtgaagg
    21781 attcgtttct tcttaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa
    21841 ggtttatagt gtctgaagct ttttttgttt gtctaaaata gccaccttca acatggggag
    21901 ctaactctag agttcttatc aagtct
//
LOCUS       DBERCA010000025        21687 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.025, whole genome shotgun sequence.
ACCESSION   DBERCA010000025 DBERCA010000000
VERSION     DBERCA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.025"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..193)
                     /locus_tag="ACL719_001136"
     CDS             complement(<1..193)
                     /locus_tag="ACL719_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699113.1"
                     /translation="MTLRTEDQVRDYAREVLGFNEVEENINQGTGQITTFNQLGFKGY
                     SDKPDGWYLPKNMNDVAIIL"
     gene            complement(197..415)
                     /locus_tag="ACL719_001137"
     CDS             complement(197..415)
                     /locus_tag="ACL719_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699114.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(1153..2784)
                     /gene="rlmD"
                     /locus_tag="ACL719_001138"
     CDS             complement(1153..2784)
                     /gene="rlmD"
                     /locus_tag="ACL719_001138"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS0699115.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2858..3832)
                     /locus_tag="ACL719_001139"
     CDS             complement(2858..3832)
                     /locus_tag="ACL719_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGS0699116.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVYAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3906..4520)
                     /gene="pmp23"
                     /locus_tag="ACL719_001140"
     CDS             complement(3906..4520)
                     /gene="pmp23"
                     /locus_tag="ACL719_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGS0699117.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4520..5494)
                     /locus_tag="ACL719_001141"
     CDS             complement(4520..5494)
                     /locus_tag="ACL719_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGS0699118.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEDVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5502..6758)
                     /gene="glyA"
                     /locus_tag="ACL719_001142"
     CDS             complement(5502..6758)
                     /gene="glyA"
                     /locus_tag="ACL719_001142"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGS0699119.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6820..7251)
                     /locus_tag="ACL719_001143"
     CDS             complement(6820..7251)
                     /locus_tag="ACL719_001143"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699120.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7253..7852)
                     /locus_tag="ACL719_001144"
     CDS             complement(7253..7852)
                     /locus_tag="ACL719_001144"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGS0699121.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7839..8678)
                     /gene="prmC"
                     /locus_tag="ACL719_001145"
     CDS             complement(7839..8678)
                     /gene="prmC"
                     /locus_tag="ACL719_001145"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGS0699122.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(8678..9757)
                     /gene="prfA"
                     /locus_tag="ACL719_001146"
     CDS             complement(8678..9757)
                     /gene="prfA"
                     /locus_tag="ACL719_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGS0699123.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9767..10246)
                     /locus_tag="ACL719_001147"
     CDS             complement(9767..10246)
                     /locus_tag="ACL719_001147"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699124.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(10236..10835)
                     /locus_tag="ACL719_001148"
     CDS             complement(10236..10835)
                     /locus_tag="ACL719_001148"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGS0699125.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALTIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10958..11140
                     /locus_tag="ACL719_001149"
     CDS             10958..11140
                     /locus_tag="ACL719_001149"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGS0699126.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            11277..12650
                     /gene="mnmE"
                     /locus_tag="ACL719_001150"
     CDS             11277..12650
                     /gene="mnmE"
                     /locus_tag="ACL719_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGS0699127.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEEVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12697..13632)
                     /gene="dapA"
                     /locus_tag="ACL719_001151"
     CDS             complement(12697..13632)
                     /gene="dapA"
                     /locus_tag="ACL719_001151"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGS0699128.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13688..14764)
                     /locus_tag="ACL719_001152"
     CDS             complement(13688..14764)
                     /locus_tag="ACL719_001152"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGS0699129.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIVACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(15350..15422)
                     /locus_tag="ACL719_001153"
     tRNA            complement(15350..15422)
                     /locus_tag="ACL719_001153"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15387..15389),aa:Thr,seq:ggt)
     gene            complement(15522..16031)
                     /locus_tag="ACL719_001154"
     CDS             complement(15522..16031)
                     /locus_tag="ACL719_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGS0699130.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(16029..16125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            16154..16585
                     /locus_tag="ACL719_001155"
     CDS             16154..16585
                     /locus_tag="ACL719_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGS0699131.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16667..17044
                     /gene="mscL"
                     /locus_tag="ACL719_001156"
     CDS             16667..17044
                     /gene="mscL"
                     /locus_tag="ACL719_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGS0699132.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(17086..18180)
                     /gene="hemH"
                     /locus_tag="ACL719_001157"
     CDS             complement(17086..18180)
                     /gene="hemH"
                     /locus_tag="ACL719_001157"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGS0699133.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVSISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            18335..19558
                     /gene="pepT"
                     /locus_tag="ACL719_001158"
     CDS             18335..19558
                     /gene="pepT"
                     /locus_tag="ACL719_001158"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGS0699134.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTTIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(19594..20244)
                     /locus_tag="ACL719_001159"
                     /pseudo
     CDS             complement(19594..20244)
                     /locus_tag="ACL719_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            20245..20409
                     /locus_tag="ACL719_001160"
                     /pseudo
     CDS             20245..20409
                     /locus_tag="ACL719_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(20680..21687)
                     /locus_tag="ACL719_001161"
                     /pseudo
     CDS             complement(20680..21687)
                     /locus_tag="ACL719_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1,RefSeq:WP_007521835.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6287 a         4521 c         3995 g         6884 t
ORIGIN      
        1 caaggattat tgctacatca ttcatatttt taggtaaata ccaaccatct ggcttatctg
       61 aatatccctt gaagcctaat tgattaaaag tagttatttg accagtacct tgattgatgt
      121 tttcttcaac ttcattaaag cctaatactt ctcttgcata atccctaact tgatcttctg
      181 tcctcaaagt cattttctat tcctcatcac taaaattatc ttcaaaacta ataatatcat
      241 taggagtgca agataaagcc ttacatattc tttctaaatt cttaaatgta attggtttat
      301 cctttcccat ttgtgccata acatttgttg taagtcctgc catagctatt acatctgttt
      361 tctttagtcc tttttttgct aactgtatcc ataatggttt atagttaagt gccataatat
      421 ccctcttttt ttcttcatta gatttattta attatagcat aaatattgat ttcattcaat
      481 cttacattga tttagtgatt tgttttagag aatttgttgt gttattccgt ccttattttg
      541 ccataaattg ttttaagtgg gacctcttat ccttaccatt tttacctcct aattttgagt
      601 ataaaaaaga cgatagagtt tttaatttct atcgtctgag atttacatat taaattttca
      661 tcacaaaata agactcttta aagaatcttt aaattcattg gtatgctaat tacaatatcc
      721 attattaaaa tggatactat aagcagcaca ccaactattt attttttgag ttttaaactt
      781 tggtagaagt cttgaactcc ttatatttac ataactattt gcctctataa ctattatgac
      841 acgaaaccaa caagcaagtt caagcacctc catatcttta ttcaaatgag gactaagata
      901 ttcacaaact ttatcataaa cctctttatc ttttttcata aaaggggcat acaacttagc
      961 aaatttatcc taaaattttt tgttgccttt atcaatcatt tcaacgctcc tttaaatata
     1021 aaaatgatcc tatcaatctt tatattgagc ctattttcta attgatacct tgcaagaaag
     1081 gaataatagc tgttcctata ccaagtagga atgtaataaa tgcagaaagt catatactgt
     1141 cattcttttc tattaaatca ttttgaagtg tctcaaagca tccatgatgg cttcttcttt
     1201 ttcaggtgta cactgtggaa taatttgttt atccttttta gacttgttgt aatgttctcg
     1261 taattctatt ccacatttct tttttatctg tgcaatatat aatgtcgaaa cttttaattc
     1321 aaatttattc caaacatatt ctttgatttg agcatatgtt gctttgctct ccgcacttgt
     1381 caaattcatc tcatccagct caatttcaac acttatgtgc ttatcgacat cgagtttgga
     1441 caaaagtgct accgtctcga cgtgatgcgt ttgaggaaat agatccaccg gctggacttt
     1501 cttcaattca tatcccaatt cttggtagag tttgatatca cgcgccatgg ttgcgacatt
     1561 gcaggagata taggcgatgc gatcggctcc tgtttgggcg cttgctttga taaagctttc
     1621 tgtcaagccc ttgcgtggag gatcaaccaa gataacggtt ggttgaatcc catctttaag
     1681 ccaattcttc atagcatttt cagctgtgtc acagacataa tgggcgtttg aaatattgtt
     1741 cagctgagca ttttttttac tattctcaac cgcttctgga atcagttcaa caccgtagac
     1801 ttctttgaca tgcttggcga ctgataaacc aatggttcca ataccagaat aagcatcaat
     1861 aaccacgtca tcttttttta actctgcaaa gtcaatggct gtttgataga gtttctccgc
     1921 catttcagta ttgacttggt aaaaggctgg gccagcgatt tggaagtcat ttcccaacat
     1981 ctggtccgta atatagtctt gaccataaag agtgcgccac tccttaccaa aaatcgcatt
     2041 ggtattctgg tcgttgatat tttgcatgac agacacaatc tctgggaact gcttgataac
     2101 ttgttcaatc aattggtcaa cacgaaaaac ttttggacga gttgtcacca aaacgaccat
     2161 gatttgtcct gaatagtgac cacgacgcac cacaagattc cgaatcaatc cagactgttc
     2221 cttttcgtca taaggtttta aatcaaaacg acggagcagg tctcgaagag ctactacgac
     2281 ttggtcaatg acaggatcct ggataaagaa atcttcaagg ggcatgaggt tgtgcgaatt
     2341 cttacggaaa aatcctgttt ccaagacacc attcactcga cgaacgggca cctgcgcctt
     2401 attgcgatac ttgactggat gttccatacc aagcgtttca gcaacttcta catctgcaat
     2461 tccagcaatc ttgtagagac tgtccttgac ttgcttggtt ttaaacttga gctgttctgg
     2521 ataagaaagg tgtcctaaat ccgcgattcc tgaacgcagg taagccaaat ctagatcttg
     2581 attacggtgt ggtgactgga caaggtattt ttcaactttt ccaaagccaa tctttttatt
     2641 gaccttgagg acacgcatga gaattttttc actcggtaaa gcattctcta caaaaaagac
     2701 caaaccatct accttggcaa ctcctgcccc ttcatgggtc aaatcaacaa tttcaacttc
     2761 tacaatatca tttttcttta acattctttt ctctttctat tggccgacaa aaaagacggc
     2821 aacgttacgg ttaccatcca ttataccatg aaatttctta agaaccaaaa ctcttgaaga
     2881 agttgacaag ggcttgccag agggagctta agaagttacc accgtcctct agcgccttat
     2941 ccgcatcaaa gttaaggttg atatttttaa aactgtcgcc agcttgtgat acgatgcttt
     3001 gtttaaggtc atttagggtt ttagtgaaat ctgcattgct gaggatatca ctctttgaga
     3061 gattcaaggc aaaattgatg atgatattga tctggtttcc tgttatgacc tgatcaagtt
     3121 tgtaattttt taaggtatct tcaacaatct tgcggatatc ttcttctgtc agatttccct
     3181 tgctttcttt agctttggcg agtcctgact tgatatcagc tagggcaacg tttaatttat
     3241 tagcatcata gcctgatttg tccttgtttt cagcattgat atctgacaaa gcctttagct
     3301 cttcttgagc caaatcttta ttggcttgcg gcaccttggc tccattagct tctagcgaat
     3361 agtagatccc agccaaagca ctctcacctg taactggaat gggtgctgct acagtgattt
     3421 tggcgtgttc cacacccaaa gttacggctg catttcggta catatcctga gttaccttag
     3481 tgatgttttc aggtgtttca atcttgacct caagtggcga tttgtcacct agcttttgaa
     3541 tcttggctga tgaatacaac tgtaagctag agtcattggc cacattcatg attttagaat
     3601 aaacatcagg tgtcatggtc ttaagttctt tggtatctgt tgaggcattg tagcccagtt
     3661 ttttaagagt ttgatttttt tggtcttcag atagggaaga acctaggaca tattcaggtt
     3721 ggacataggt ttcatcgata actttttgaa catctgttgc tgcatagacg ctattcatag
     3781 ctgttactgc ccacaagatc gcagcgctag tcagaaagag tttctttctc atagggaatt
     3841 tcctccttta cttctttaga gtaatatatc tatcttaaag aaaacttata acaaaaacac
     3901 ctggtctagc cagatgttga aaagagagtg aaacatttga tgatgtaaag gttaagtcgt
     3961 acctgtctag aataataata gtttcctcca tttacataga gttcagcacc gtgaaaaatg
     4021 gaaatgggat gaatataact ataagtcttt ccagtcgtat taccaagcaa gggggcaaca
     4081 gtctcacgag agtactgttt ggctagagcc aaggtatttt ccttgccatt ttgggcgata
     4141 aaatcgatat aggcaggtcc aaaattatag gcttgaacag ctgtccagat atctacaccc
     4201 ttcttctgcg ccagatagag attgcctgtc agagtttgaa tgccttgccg aatgctagag
     4261 gcattatcat tgatggtgtt ggtggaacca cttgcagact cactagactg cataacatcg
     4321 ccttcttttc cttttgtttc agtataaatc atagcaagca caagctcttc gtttgctggg
     4381 gtgtcttttt cactcaatat ttctcgcacc atgggttgat aggtcatgac ttgtttgaca
     4441 tcttgatgaa cgcggtaagc tttatagcca gcaaaaagga agactgctag tacaagcact
     4501 cttcgaattc gtttaaacat tatttacttt ggatatcctc gatatttttg attaagatag
     4561 agtaggttcc attttcgttt tggataaact caacagactc ggcgtcttga tagacgttat
     4621 tgggaacgat gagctcaatt ccatttgata aggagagttt ttggttttca aatttcttta
     4681 attggcgact ggcatcaatt tcatcaaatt gaacaggttc tggtacgtct tctttgactt
     4741 ggtcaataaa gctcaaacga gccgtcagat tgttgtcaaa aaggtcatta gccaatttct
     4801 caggtgacaa ttcattgctt tcttctaggt tgttgaaaat cgctgatttg accttggatt
     4861 gaaattgaaa atcatctgtg ttaaaagatt cagcaattct ctgggctgtt ttttccagtt
     4921 ccttgataga ttttttagga gaaatcttag gagcgacagc aagaagatta tctgaaaaat
     4981 agttcaaaaa agtcccgttg tacttgattc gtttttcaat caggtgatac ttgcgactct
     5041 gaagattgac caccaaggcc tcgtcagcac ccgttccaaa tccaggcagg ttattctgag
     5101 tcagcttgat tggattatca acttctcctc cgaggtgggt caaggtctcc cgcagggcaa
     5161 ttcgcaagaa agcgaaatgt tctacacctt ctttagaaaa ttgaacaaaa atcaagtcat
     5221 tggtcttgag attttcagaa atgctaaact cctctttcca gagattagcc agcgttactg
     5281 atgtctccaa caaatcgtct gtaatatgat tgaagaaggg attttcttct tcgaaaatcc
     5341 cagtcttggc ttcatctgaa tacacatgtt caattttttt acgtaggtat tcttcgattt
     5401 ttggagtaat attgagaaac ttatctgcta agaacagctc ggtatcatcc ggactgaact
     5461 ggtgaataat ggctttctta atataaatat ccataaaagt tttagtcctc gtataatggg
     5521 aaggcatctg tcaattcttt gactgcactt ctcacttctt ctaatacagc ctcattttct
     5581 gaattcttaa gggttttaat gatgagttca gccactttgc gactttcttc ttcaccaaat
     5641 ccacgtgcag taatagctgc tgctccgata cgaatcccac ttgtcttgaa tggcgacaag
     5701 gtttcgtaag ggattgaatt tttatttagg gtaatattga cttcatccag caagttttga
     5761 gcaacttttc cgttttctac aaccttagtc acatcaacaa ggaagagatg gttttcagtt
     5821 ccacctgaaa taatacggaa atcagggtct tgcaagaaga catctgccat agccttgctg
     5881 ttcttaatta cattggcagc atattccttg aaggctggat ccaaaacttc tttgaaagaa
     5941 actgctttag ccgccacaac atgctctaaa ggaccgccct gaatacctgg gaaaatagct
     6001 gaattgattt ttttagcaag ttcttcgtca ttggtcaaaa tcaaaccacc acgaggtcca
     6061 cgaagggttt tgtgggtcgt tgttgttgtg atatgagcgt atggaactgg gcttggatga
     6121 aggccagccg caaccaaacc agcgatatgg gccatgtcca ccatgagctt cgcaccgaca
     6181 gcatctgcga tttcacggaa ttttgaaaaa tcgataattt gagaatatgc tgaagcacca
     6241 gctacaatca gttttggttt tacttcttgg gcttgtttca agatagcatc aaagtctaag
     6301 agttccgttt caggatcaac actataagaa acaaagttgt aggtttgacc agagaagcta
     6361 acaggagccc catgagtcaa atgaccacct gatgccaaat ccattcccat aaccgtatca
     6421 cctggctcaa tcaaggacat gtaagccgca cagttagctt ggcttcctga atgtggttgg
     6481 acattggcaa atttagcacc gaaaatttct tttgcgcgtt caatagcaag agtctctaca
     6541 acgtctacta catcagttcc accataataa cggcgtcctg ggtaaccctc ggcatattta
     6601 tttgtcaaga tagacccttg agctgccata acagccttgg aaactacgtt ttccgaagca
     6661 attaactcga tattattttg ttggcgttct tcttctttgg caatagcatt ccagagatca
     6721 gcatcatatg ctttaaaatc atctttgtca aaaatcatag gtcttctcct ttattgtgtg
     6781 actagtccat tagtttgatt ttacaataag aaaatcaaac taaaagatgc gaataaaccg
     6841 tttctgcatt ttatcacaag tatagccaac tttttcataa aatgcatgag cacccagacg
     6901 atgattggca gaatttaagc ggataaaccc ataaccacat cttttggctt cttgttccaa
     6961 cccttgtagt aaacttttac cgataccttg accttgcgct tgaggtgaaa ctgctaaagc
     7021 taagatatta aatcctgctt tggaatagag tgattcgtaa acttcagcgt ggacatatcc
     7081 aagtaagaca tgattagctg catcctcata gccaagtagg aaatgatggg aatcctgaga
     7141 cagtctagct agttggctag ccgtttcctc tggactaaaa gtataaccca aagcctcttg
     7201 gttgatgtca catatcgctt tcacatctgt ttcttgcaaa tctcttagca tttcaagcct
     7261 cctcaaaaga aatctctggc aaccgagcaa gaatatcttc tcgtttaatt gcgccttggc
     7321 gtaagatttt caccttgtct ccagacaaat ccacaatagt tgaatcctgt ccagttagaa
     7381 aagcatcgtc ttccagaccc agaacctctt ggtcaaaatc cttcagaatt tgttcaaagg
     7441 ttacaccact tgcctgacct gagatattgg cagacggccc aatcaaggga cctgtctctc
     7501 gaattaaatc cagtgtgata gggtgactgg gcatccgaaa tccaatagtt gcaaggtcag
     7561 aatttaccca atagggaact cggtcattgg cttcgagaat aatggtcaag ggacctggca
     7621 aaaaggtctc tacaagtttt tgtagataag ctggctgatt cttagaaaag tgcaagatat
     7681 cctctaaaga ggcaacattg agattgagcg ccttgtctct aggacgacgt ttgagctggt
     7741 aaacatggtc aactgctttt tcgtctaagg ccttggcaaa aagaccataa accgtctctg
     7801 taggcagaat gacagctcca ccattttcca actcttgtct aatcctgtcc atcatcaacc
     7861 acgaccatcc tatcttgacc aaattggtcc ttgagtgttc gaactttttt ttcaggaaga
     7921 tgttttctaa aaagttcggg aacactttga ccttgcttgt atccaatttc aaggtaaatc
     7981 ttaccaccat ctttgagata gtcttttgca tcttccgcaa ttctacggta aatagctagg
     8041 ccatcctcgt ctgcaaagag agctagatga ggctccgaat acaagacatt caagcctacc
     8101 tctgactcat cttcacgaga gatatagggt ggattggaaa caattatatc atatttttca
     8161 gaaatttctg taaaacagtc agatttttta aaaaatattt gaagattttg atttttagca
     8221 ttttcgctag ctaaatctaa agcctcttga gaaacatctg ctgctgtcac tgaccaatct
     8281 ggtctatttt ttgctagagc gagagcaata gctccactac ctgttccaat atccagaact
     8341 gaaagattcg tctcaagatt ttcagttaga ataagctcca ctaactcctc tgtttctgga
     8401 cgaggaatca aaacccgttc atccaccttt aaatgcattc cataaaaatc tgcctgtcca
     8461 atgatgtact gagctggctt gtgagctgct agttgctggt aaatatcttc tacaaattgt
     8521 ttttcttcct ctgttgtcac ctcctgctgg agggcaaaaa taaagtctgt aaaagataga
     8581 tttttcagac tacgatagac aaaagagagg ctttccgctt cctctccttg tcttatcaac
     8641 tcttcttcaa aatttgaaaa taattgagct aatttcatta tttgtttaat tcttctagtt
     8701 tttgtgtttg gtcataaagc accaaggcat ccacaacttc gtccaattta ccagacaaaa
     8761 tcgtatctag tttttggagg gtcaagccga tacggtggtc tgtgacacgg ttttgtggga
     8821 agttataagt tcggatccgt tctgaacggt caccagtacc gattgtcgac ttacgctcag
     8881 cgtcttgttc atcctgagca atctgagcaa agtggtcagc gacgcgtgca cggataatct
     8941 tcatagcctt ctcgcggttc ttctgctggg tacgttcttc ctgcatctca accttgatat
     9001 tggttggcaa gtgaacgata cgaacggcag tcgcaacctt attgacgttc tgtccaccag
     9061 caccagaggc gtgatagatg tcgacacgaa ggtcttttgg atcaatgtcg tattcaacct
     9121 cttcaacttc tggcataaca agaactgtcg ctgtcgaagt atgaacacgg ccttggcttt
     9181 ctgtcacagg aacacgttgc acacggtggg cacctgattc atacttaagc ttagagtata
     9241 cagactgacc tgaaaccata gcaaccactt ctttaaaacc accgacacca ttcatagagg
     9301 cttccatgac ttcaaagcgc caaccttggg cttccgcata cttttggtac atagttagca
     9361 aatctccagc gaaaagtgcc gcttcgtctc caccagctgc tccacggatt tcaaggatga
     9421 tattcttgtc atcgtttgga tcctttggaa ggagcaaaat tttcagtttt tcttcatatt
     9481 cttctttttc agccttggca tctttgagtt cttgcttggc catttcttcc aagtccgcat
     9541 ctccgcctga ttccttaatc atctcttcgg catcgacgat attttgaagg acttgtttat
     9601 actcacggta ggctattacg gtgtcacgat tggaagcttc ttcttttgaa agctccataa
     9661 aacgcttggt gtctgaaacg acatcagggt cactcagcaa ttctcctagt tcttcataac
     9721 ggtcttctac agcttgtagt tgatcataga tgttcatttt ttctccttat ttctcaattg
     9781 ttaaatcata gattgctact acttcattct cggatatttc cccagtttct ttaaatccat
     9841 aactgagata acaaaatctt gcctgttcat tttctggttc ataagacaac caaagtttat
     9901 tgcttaaacc tgctggcgct gttcgaacat agtctagtac tttatccata attggtttaa
     9961 aatatccttg attttgaaaa ttcttatcaa tcataaaacg aaatagtaaa taatttccac
    10021 tactaattac gatcttttta tcataagcta tcatcacaaa acctataatt acatcattat
    10081 cataaactgc caatggagct acaaaatctc catttttagt gtagacgtat gcttcagcta
    10141 aactaattgc gttggttgca atgaattgtt tttgatattc cttgacatcc aaatttaaaa
    10201 catcaaaata attttccatt gtaacatctc ttagttcaat tgtcatagtt ttgctccttg
    10261 ttagaggtta tcattggcgc aaaataatgt ttacggcaaa ccgaaatata ggtttcatta
    10321 ccaccaatct ggatctgttc tccatcgtaa acgggcagtc catcctgtgt tcgcaacacc
    10381 atggtcgcct ttttcttgca atactgacag atggtcttga tttcgtcaat cttgtctgct
    10441 aaaagcaaga gatatttgga accttcgaac aattcattgc gaaagtcatt tttcaagcca
    10501 aaagccatga cgggtatgtc taactcgtcc acaacacgag ctaggtcgta aacatggtgg
    10561 cgtttgagaa actgggcttc atcgaccaaa acacagtaag gtttttctgg taggtctcgg
    10621 atatagccaa agatatccgt tgtttcctca atcgtaaggg cagggcgttt catgccaatt
    10681 cgactcgaca catagccaac accgtcacgc gtatccagag ccgaagtcat aatgacaaca
    10741 ccttttcctt gctcctcgta gttataggcc actttgagaa tctcaatcgt tttaccagag
    10801 ttcatggtcc cataacgata atacaactgt gccatgtttc ttgcttcacg tccatttcta
    10861 aatttttgct acattctagt atatcataat tttcttaagc tttaaacggc aaaatgtggt
    10921 aaaatggaag aaatcaaaac tagtggagga agctattatg ccatttgtac gcatcgattt
    10981 atttgaagga cgcacgctcg agcaaaagaa agctcttgct aaggaagtaa cggaagcagt
    11041 tgtccgcaac actggagccc ctcaatctgc tgtccatgtc atcatcaacg acatgccaga
    11101 aggaacttac ttcccacaag gggaaatgcg tactaaataa gctagcttaa gcagaattgc
    11161 ttaggctttt tcaatctcca agtagcattc attgaagaaa tatcctaaat ttgttacaat
    11221 ttgaaaagaa acttggagaa tttccaagaa aatagctatt aattaaagga aacattatga
    11281 ttacacgtga atttgatacc atcgctgcta tctctactcc actaggtgaa ggggctattg
    11341 gtattgtccg cctgagcgga acagacagtt ttgctattgc gcaaaagatt tttaaaggaa
    11401 aagacttgaa caaggttgcc agccacactc tcaactacgg tcacattatt gatcctctga
    11461 ctggtaaagt catggacgag gtcatggtag gagccatgaa atctccaaaa accttcactc
    11521 gtgaagatat tatcgagatt aacacccacg gtgggattgc ggtgaccaat gagattctcc
    11581 agctagctat ccgtgaagga gctcgattgg ctgaacctgg tgaatttacc aaacgtgcct
    11641 tcctaaacgg tcgcgtagat ttgacacagg ctgaggcagt gatggacatc atccgtgcca
    11701 agactgacaa ggccatgaac attgcagtta agcaattaga cggctccctt tctgacctca
    11761 ttaacaatac ccgtcaagaa atcctcaata cacttgccca agttgaggtc aatatcgact
    11821 atcctgaata tgatgatgtt gaggaagcta ctactgctgt tgtccgtgag aagactatgg
    11881 agtttgagca attgctaacc aagctcctta ggacagcccg tcgcggcaaa atccttcgtg
    11941 aaggaatttc aacggctatc attggacgtc ccaacgttgg gaaatcaagc cttctcaaca
    12001 acctcttgcg tgaggacaag gctatcgtaa cagatatcgc tgggacaaca cgagatgtca
    12061 tcgaagagta cgtcaacatc aatggtgtac ctctcaaatt gattgataca gccggtattc
    12121 gtgaaacaga tgacatagtg gaacaaatcg gtgttgagcg ttcgaaaaaa gccctcaagg
    12181 aagccgactt ggttctacta gtgctaaatg ccagtgaacc actgactgcg caagacagac
    12241 aacttcttga aattagccaa gataccaatc gcattattct acttaataaa accgacctgc
    12301 cagaaacgat tgaaacttcg aaactacctg aagaggttat ccgtatttca gtccttaaaa
    12361 accaaaacat cgacaagatt gaagagcgaa tcaacaacct cttctttgaa aatgctggct
    12421 tggtcgagca agatgctacc tacttgtcaa acgcccgtca catttccctg attgaaaaag
    12481 cagttgaaag cctacaagcc gttaatcaag gtcttgagct ggggatgcca gttgatttgc
    12541 ttcaagttga cttgactcgt acttgggaaa tcctcggaga aatcactggg gatgctgctc
    12601 cagatgaact tattacccaa ctctttagcc aattctgttt aggaaaataa gaaaaatcca
    12661 tgatccttca ttcggtcatg gattttattg tctttattag taatctggtc ttaagacccc
    12721 tgttacagtt gccttagttg cttcgtagtc gccatctacg acaaccttga taatgcgttt
    12781 ggcatcttct tctggtgctg gaacaagagg tagacgagtg ggtccagctt caaatcccat
    12841 atagttaaga actgccttaa ctggagcagg acttggataa gagaagagag cattaacctt
    12901 aggaatgaat ttacgctgaa ttgctgcggc tttcttcata tcgctttctg caatggcagt
    12961 aaacatctcg tgcatttcat ccccatttgt atgagaggca acagaaataa ccccatccgc
    13021 cccaaggttc atggcatgga aagcatctcc atcctcacct gtataaatca agaactcttc
    13081 aggcttgtgc tcaatcaaat aagccatatt agccaagcta gtacattctt tgacaccgat
    13141 aatatttgga tagtcagcca agcgaagcat ggtttctgga gtcaattcga caactacacg
    13201 ccctggaatg ttatagataa taattggtag gtcagaagca tctgcaatag ccttaaagtg
    13261 ctgatacatc ccttcttgag aaggtttgtt gtagtaagga acaatagcaa gcccagctgc
    13321 gaaaccacca aattccgcta cttctttgac aaactcaata gagtcacgcg tatcattagt
    13381 acctacaccc gcaatcaaag gaacgcgtcc attgacaacc ttttgtacag ccgcaaacaa
    13441 ctccaactcc tcatcgtggg tcaaagttgg actctcagca gtcgttcctg cgagaagaat
    13501 tccatccgta tgatgggcca ataaatgctc aatcaaggct ggaatagcat caaagttaat
    13561 ggaaccatcc tcatggaagg gggtaataaa ggctgtaatg attttacatt cttttaaatc
    13621 ttgataagac acgaaaacac ctctctattt caaagaagga gttcatctga actcctaaac
    13681 gatatgacta ttttaattca aatttcaatt cggctgttgg acgaaccaat ccacgttcat
    13741 gaagagtttc agcaatctga actgagttcc aagcagcacc tttgagaagg ttatctgaaa
    13801 caacccacat gtgaattcct ttttctgcat ccaagtcttt acggatacga ccaacaaagg
    13861 tatcacgcga accaactgca ttgatggctt gaggatagat ttgatgagct acatcatctt
    13921 caagaacagc acctgggaag gctgcgatag ctgcttttac ttcttcgatt ggagccactt
    13981 cttttgtttc gatataaaca gactcagagt gagctgacaa gactggaata cgcacacatg
    14041 ttgcagatac tgcaatgcta tcatcttcca taattttctt agtttccttg gtcatcttca
    14101 tctcttcgta cgtgtaatca ttatcagtga aaacatcaat ttgtggaaga gcgttaaagg
    14161 cgataggata atgtttcttg tcaccacctg aaggcaaaat ttccgcatgc aaatcacatg
    14221 gtttcacacc atcattcaag acttcacgaa gttcacgttg tgtctcaaga attgctccca
    14281 taccagcacc tgaaacggct tgataagttg aaacaatgat acggtccaag ccccattttt
    14341 ggcgaaccgg ctcaagagcc accatcattt ggattgttga acaattaggg caggcaacga
    14401 ttccgttgtg agcatcaagt gcatgagcat tgacctctgg aacaaccaaa ggaacatctg
    14461 gattttgacg gaaataagat gtattatcta ctactaccac gccagctttt actgcgtatg
    14521 gtgcatactt agctgatgta gaaccacctg ctgaaaagag agcaatatca actccttcaa
    14581 aagctgtttc agtcgtttct tcaatcgtaa tatcttgatc tttaaatttc aatgacttac
    14641 ctgctgaacg tgcagaagca aggtaacgga ttttatcgat tggaagtgtt gattcttcca
    14701 acatttttat catctgagca ccgacagcac ctgtcgcgcc gactacagca actgtatatc
    14761 ccataaataa cctctttcgg aattttctaa aaatttctat aatagaagta ttatactact
    14821 ttttccatga attgcaaagc tttttcatca ttttttggaa aataaaaatc cccaatcgct
    14881 agactaggga ttaagaggca tcttcatgtg atgagcaggt tcacacaact catcaaggtc
    14941 cgctcctgcg ttatgacctc cttatgctca atagtagccc gaagactacc tatcgactca
    15001 tcgcatctac tattctacta aagagaatga cttttgtcaa cagacgaaac tctttatact
    15061 cttcgaaaat ctcttcaaac ttcgtcagcg tcgccttgcc gtatgtatgg ttactgactt
    15121 cgtcagtttc atctacaacc tcaaaaacat gttttgagct gacttcgtca gttctatcta
    15181 caacctcaaa gcagtgcttt gagcaacctg cggctagttt cctagtttac tctttgattt
    15241 tcattgagta ttagttttat ttacacaggt gcataagaaa tatattggaa aaattggggg
    15301 cagacataac aaaaaccctt gtaaatcaag ggcttttctg tctatttaat gctaattgcc
    15361 gggattgaac cggcgacctc atccttacca tggatgcgct ctgccaactg agctaaatca
    15421 gcttacttaa gaagtatact ataatcagaa aaacttgtca agtttcctta gaaattttcc
    15481 ataggaaaaa gccaggtaag aactacctag cttatcttct tctatttact taaatcaatt
    15541 cgacgaccaa ttttaccaat aataatcgct ccaataatca atgaagcaaa ttcaccgatt
    15601 cctgttgtaa accaagtaag gaagaatggt aattctgcta caatatttaa ctctgcagcg
    15661 atagtaaaca tggaaattga aaagaggatt gaaaagaaga aatgatcttt tcgaatcaat
    15721 ccgttaaaga ggtaatcctt gctatatttt gcaaaaagcc aaacacctaa actgaggaat
    15781 actaaggttg aaccaccacc aacaaagaca tctagcagtc cgaagctaaa gaaattagca
    15841 atcatacaac cgatggtaac tccgatgatg tacttaggat tgtaaaaagc cataaagttc
    15901 atcatttctg aaatacgaaa ctgataagca ccatagctga tggcatttag gggcggtgtg
    15961 acagtcaaaa cgacgtagat agcagcgacg attgcaatat ctgcaacatc acgaatagtt
    16021 aattttttca tcttttctcc tttggcggtt atccgcgtgt aatatgcttg gtgaaagaag
    16081 ctaagcacca agggttgatt cattcaacct tactagtata gcacaatagc ctgctttatg
    16141 ctatacttaa agcatgaaaa aacctattca aaaatttttt aacaatgaaa tcttagccta
    16201 tctctttttt ggccttgcga caactctagt ttcgattctc tcacgactag tcatttatca
    16261 actaagtcat caggaactcc ttgctacggc cttggcaaat attatcggta tcttatttgc
    16321 ctttatcaca aatgatacga ttgtatttaa gcaaaaaaag aaaaatagac ttatccgttt
    16381 agtgaaattt tctcttgctc gcctttctac ttttctgtta gacttgtttt taacttttct
    16441 ctttgtaacc caatttcctc acatcatagg gcaattcgta actgaaaaca ttgataagat
    16501 aaatgctatt gaaacaataa ttgcacaaat attcattatt atcatcaatt atatcttgag
    16561 caaggtctat atttttagaa aataatgctc catttatgag ccttttaatt gccttctata
    16621 atgatttagg atacaatgaa acacgaaaca ttgaaaggaa aacagtatgt taaaaaatct
    16681 aaaatcgttc ttgcttcgag gaaatgttat tgaccttgct gtcggtgttg tcattgcctc
    16741 tgcttttggt gctatcgtta cttcacttgt aaacgacatt atcactcctc ttattttaaa
    16801 tcccgctttg aaagctgcta aagtcgaacg tatcgctcaa ctttcttggc atggagtcgg
    16861 ctatggtaac tttttaagtg ctattatcaa ttttatcttt gtgggtaccg ccctcttctt
    16921 tattatcaag ggcattgaaa aagcacagaa actgactggc ataaagaaag aaaaaactgc
    16981 cgaaaaaaaa ccaaccgaat tggaagtcct tcaagaaata aaagctctcc ttgagaaaaa
    17041 ataatcgatt aaaaggatct agcgcaaagc taaatccttt tttctttact ctttgggtaa
    17101 cttgcctgct ttttcaagca tttttttgat aagataaggc atcttaacat tctcccgccc
    17161 ttttttctca atcagttttt tcacaaattc tggcatttgt aaatcttcag attcagctaa
    17221 aatgtttttc gtctcgtctg aaggaactaa ctcatcaaag gtctcttctt ctgggagaaa
    17281 ttccttaaac tcttgatttt cattgactct aactgtattg accaaagcat caatcaaacg
    17341 agaatctgta tttggcattg gtggacgatg gtagtttacc ccaaattcct ggcataagtc
    17401 ataacattct acatcattgt caaacaagac ctcaatatgc tcactgatga aactaataga
    17461 tacaaaaata taatggtctg gatgttctgt ctgttctctg agatactcca aaacatctgg
    17521 tttaatccat ggaataccaa tatcactctc actctgccaa gtgttggtat attgttctga
    17581 actcaagccc agtttttcag cgactagctt gctattttcg aaaatttggt ctatataagg
    17641 atcaccaaag tccaaggcaa aaatgggcac actatgggct gaaaagatga ctttaaagct
    17701 gtcttgcttt acttcttctt ttaaaatctt agcaatttca tctgcccaat agtttaagag
    17761 cgcttcttcc tgataccagt ccttaatgac caaaaattga atttgtttgc tttctaaaaa
    17821 tttttcatag cccatgacag agtagaaaga ataatggggc tccaaaatca agcaaataca
    17881 ctgttcaatt ccgtctgctt ccatttgacc gatcacatct ggaataaagg gactggaaaa
    17941 cttattagca aagtagacac tgtattcatt ccccagccta gcttctacca aggcaacctc
    18001 ttgacgggta attttttgca gaggcgtgcc tccaattcgt acataattat catagagagt
    18061 ctgaatctcg tggtcttggg gtctcacccc acgacgaata tttgtgaaaa aatcagctac
    18121 accttcaaag gtaatctctt ctggtgaacc gaaggtcatc attaaaattg cttttttcat
    18181 gactgcttcc ttgtgatgtt tttatcaagt ttattttatc attaattcat cgataagtaa
    18241 acgctaacat agggacattc tcaatcttct gtcttttgtt tttctcttct ttctatgata
    18301 caatggaaaa aataaattca aaaggagttt ttttatgact tatccaaatc tcttggaccg
    18361 cttcttaacc tacgttaagg tcaacacgcg ctctgatgaa cactctacta ctactccaag
    18421 tacacagagt caggttgact tcgcaacaaa tgtcctaatt cctgaaatga aacgtgttgg
    18481 actgcaaaat gtttactatc taccgaatgg ttttgctatt ggaaccttgc cagccaacga
    18541 tccgtcttta acacgtaaga ttggttttat atcgcacatg gatactgctg attttaatgc
    18601 tgaaggagtc aatccacagg taattgaaaa ctacgatggt ggtgtgattg aactagggaa
    18661 ttctggtttc aaactcgatc cagctgactt caagagtctt gaaaaatatc caggacaaac
    18721 gctcatcaca acagatggaa caagcttgct aggtgctgat gacaagtcag gaattgctga
    18781 aattatgaca accattgaat atctaactgc tcatcctgaa attaagcact gtgagattcg
    18841 tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc aataaatttg atgcagaaga
    18901 ttttgatgtg gattttgcct acactgttga tggtggtcca ctaggtgaac ttcagtacga
    18961 gactttctca gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc accctggtac
    19021 tgccaaaggg cagatggtca atgcccttca gctagcaatt gattttcata atcaacttcc
    19081 agaaaatgac cgacctgagt taactgaagg ttaccaaggt ttttaccatc taatggatgt
    19141 gacaggtagt gttgaggagg tgcgtgcaag ctacatcatt cgtgattttg aaaaagatgc
    19201 ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg aagaacttgg
    19261 gagcgaccgt gtcactctca acttgacaga ccagtactac aatatgaaag aagtcattga
    19321 aaaagatatg actccaatta ccattgctaa agccgttatg gaagatctag gtatcacgcc
    19381 tattatcgaa ccaatccggg gtggaacaga cggctctaag atttccttta tgggaatccc
    19441 aactccgaat atctttgcag gtggcgaaaa tatgcacgga cgttttgaat acgttagcct
    19501 tcagactatg gaacgtgcag ttgataccat cattggcatc gtagcttata aaggctaaaa
    19561 agacgaggta gctcagctac ttcgcctttc tttttattct actggttttt cttgatttcc
    19621 agtagttgta gaagattctg ttgtttcatt ttctgaagtt gattcagcag gtttagaatc
    19681 tcttgtattg cttggtttgt tttcgtcgct agcagtttca atgttagatt ctgcagttgc
    19741 gtttggttgg ttctcagcac tggtgttatc actatttgct tcagcatttc ttgctggact
    19801 tgtttcttca cttgcgctag cttttgactg gatttgatga ttcaaaacta gaatagcttt
    19861 tgtcgattca agtaaagctg ttttgtcttt actcttagca gaaagttgat ctaataatgc
    19921 atccacctta tcaaagtccg catcagatcc attattactt tctaaataag agtgaagcga
    19981 catgagaata tcgtagagtt tttgatagag tacaagtgtc tgagaatctt gctcagcatt
    20041 ttccttttct tgttgaaggg cgctagcgat acgagtcaaa acatctttta cctgactgtt
    20101 tacttcatcc aagtctgcat cagccttgtt tgtggcagct tttagatttt ctacttcttc
    20161 tgccaaggat tgtctgattc cttcttcatg gatttgttcc aagagttgat ttgcattgtt
    20221 caaaagactt tctacttctt cctttctatc tgtcgcagat tattggttgc tatctaccat
    20281 gtactcctaa aacaggagag ttataatcca agattacaag gccttacaga aataagaaat
    20341 ccagataaga caatgttcgt ccaagacgct attcgcttcg cacagcagca cagattcaat
    20401 atactttaat tttaaagttt aggtgtcaag acctcttttt agtgtgccca aaatttagag
    20461 aagtaatcaa tcaactaact tttatttttt tcaaactttc agtaaactga cctaaagcta
    20521 actcaatctg tctttgtaga tgcttctgct atcagctaga agttgatcta cttttgccaa
    20581 gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg gaatcttgtt
    20641 tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt ggtcgccatg
    20701 aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa aaaccatgac
    20761 catcttcttc ctcatggtgg acatgtagtg acggattact taatacagaa ctagaagaac
    20821 ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt aagagatgac ttaggaatat
    20881 agtgatagtg ttccccatgt cttactatat aagcatcacc tatatctctg acaatatcat
    20941 tagggttaaa gacatatgtg gctgctaatt cacctgccga caagtcactc tcaggaatga
    21001 aatgatagtg accaccatgt ggtactatag taggttgaaa tagaatatga gcaaattgat
    21061 aaggggattt taaagtaatt tctaacaatg atttagaaac tatgatgtgc tattctaaat
    21121 tcaactcact atatataacc atcatcggta gtataacgtc cctgtaattt tgctacagat
    21181 acttctgcac tagctccttt atcgtcttta ccatgttctt gtttttggcg attgatttca
    21241 tcttttgttc gtacattttc tgcatgagct tgatctttaa ggtaaacata atactttcca
    21301 tctaccttaa taatatatcc tcccttaacc taactgacga tatcttgatc tttcggctga
    21361 tagttggggg ctttcattag tagctcttca ctaaagagcg catcaaaagg aactttacca
    21421 ttatagtagt gataatgatc gccatgagaa gttacataac cttgatctgt aatcttaata
    21481 acaatttgtt ttgcttgaat tccttctttt tgactaacct agtctggagt caaattttca
    21541 gtcttcttag tgtctttatt actgtttaca tatgaaacac gatttttatc tgtattggcc
    21601 tgttagctat gttggttcag agcataaaca cacagactta aggaaaggat aacaacagat
    21661 ccagctgcta tatatttctt tttaaat
//
LOCUS       DBERCA010000026        21539 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.026, whole genome shotgun sequence.
ACCESSION   DBERCA010000026 DBERCA010000000
VERSION     DBERCA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.026"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            296..622
                     /locus_tag="ACL719_001162"
                     /pseudo
     CDS             296..622
                     /locus_tag="ACL719_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(674..1261)
                     /locus_tag="ACL719_001163"
     CDS             complement(674..1261)
                     /locus_tag="ACL719_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS0699135.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            1503..2174
                     /gene="sdaAB"
                     /locus_tag="ACL719_001164"
     CDS             1503..2174
                     /gene="sdaAB"
                     /locus_tag="ACL719_001164"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGS0699136.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDDAIEEIRKIPHLHN
                     VNFFK"
     gene            2183..3055
                     /gene="sdaAA"
                     /locus_tag="ACL719_001165"
     CDS             2183..3055
                     /gene="sdaAA"
                     /locus_tag="ACL719_001165"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGS0699137.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            3077..3709
                     /locus_tag="ACL719_001166"
     CDS             3077..3709
                     /locus_tag="ACL719_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0699138.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(3967..5817)
                     /locus_tag="ACL719_001167"
     CDS             complement(3967..5817)
                     /locus_tag="ACL719_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGS0699139.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(5856..7016)
                     /locus_tag="ACL719_001168"
     CDS             complement(5856..7016)
                     /locus_tag="ACL719_001168"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0699140.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            7074..8240
                     /locus_tag="ACL719_001169"
     CDS             7074..8240
                     /locus_tag="ACL719_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGS0699141.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            8379..8705
                     /locus_tag="ACL719_001170"
     CDS             8379..8705
                     /locus_tag="ACL719_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS0699142.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            8692..9284
                     /locus_tag="ACL719_001171"
                     /pseudo
     CDS             8692..9284
                     /locus_tag="ACL719_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
     gene            9277..10188
                     /locus_tag="ACL719_001172"
     CDS             9277..10188
                     /locus_tag="ACL719_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGS0699143.1"
                     /translation="MRKWTKGFLIFGVATTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSANHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            10251..11315
                     /locus_tag="ACL719_001173"
     CDS             10251..11315
                     /locus_tag="ACL719_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGS0699144.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEERAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11396..12247)
                     /locus_tag="ACL719_001174"
     CDS             complement(11396..12247)
                     /locus_tag="ACL719_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGS0699145.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLA"
     gene            complement(12369..13355)
                     /locus_tag="ACL719_001175"
     CDS             complement(12369..13355)
                     /locus_tag="ACL719_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGS0699146.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(13627..15102)
                     /locus_tag="ACL719_001176"
     CDS             complement(13627..15102)
                     /locus_tag="ACL719_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0699147.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKSVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFTFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(15206..16129)
                     /locus_tag="ACL719_001177"
     CDS             complement(15206..16129)
                     /locus_tag="ACL719_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0699148.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(16143..17072)
                     /locus_tag="ACL719_001178"
     CDS             complement(16143..17072)
                     /locus_tag="ACL719_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699149.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(17391..17612)
                     /locus_tag="ACL719_001179"
     CDS             complement(17391..17612)
                     /locus_tag="ACL719_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699150.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(17566..17826)
                     /locus_tag="ACL719_001180"
     CDS             complement(17566..17826)
                     /locus_tag="ACL719_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699151.1"
                     /translation="MTGVSNPTTDSARLVLADSKKAFADDSLIEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            17906..18043
                     /locus_tag="ACL719_001181"
                     /pseudo
     CDS             17906..18043
                     /locus_tag="ACL719_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(18147..18302)
                     /locus_tag="ACL719_001182"
                     /pseudo
     CDS             complement(18147..18302)
                     /locus_tag="ACL719_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(18487..19098)
                     /gene="rpsD"
                     /locus_tag="ACL719_001183"
     CDS             complement(18487..19098)
                     /gene="rpsD"
                     /locus_tag="ACL719_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGS0699152.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(19293..20345)
                     /locus_tag="ACL719_001184"
     CDS             complement(19293..20345)
                     /locus_tag="ACL719_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0699153.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(20342..21040)
                     /locus_tag="ACL719_001185"
     CDS             complement(20342..21040)
                     /locus_tag="ACL719_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0699154.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(21207..>21539)
                     /locus_tag="ACL719_001186"
     CDS             complement(21207..>21539)
                     /locus_tag="ACL719_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS0699155.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT         6370 a         4638 c         4015 g         6516 t
ORIGIN      
        1 tttctcctat ctttattttc tacctacatc atacctctta ttaagctcat tttaaaatta
       61 cgttaacttt tttcacgaaa aggagaaaat tatgcgttat tattatggat agcgctttca
      121 aatttttaaa ctcccatccc atccttttat ctatataata agtgaaaata taataactgt
      181 caagtaatcg aagtgaattt tataaaaaaa attacaagcc aaatttgcaa agtttacaat
      241 aagccgctag ggcaatcgtc tatcagaatg tccgtttatt tgtcaataat ccgagaaaat
      301 cttgcaacgc ttagaagtct ataaaaacta tcaacattta tatgacttgc gaatagcaat
      361 cctgctcaac ctttccacac tctatctata caatcaagat aaaaacatgt gtaagcaaat
      421 ctgctacact ttactggagg acgccaagaa taagaaaagc tacgataggc tggctatctg
      481 ctatgtccct attgggattt gtacagacga ttctaaactt atccaaaaag ggttctccct
      541 tctggagcta accgaggaaa cttctatgct gtctcatctc aaaaaagaag tagagatcta
      601 ttaccaagcg aaggaaagat aaaaaagccg agggatttcc tcgactcttt acatattatt
      661 tgttcgttag ttcttaatac cagccattgt taagccagaa gtttttagca gcagtccatg
      721 aaccgtaacg tcctgcaacg taggcatctg ctacacgttc ttggttttca gctgagtagt
      781 caccgttcag gtatgaatct gttaattggt aacgtccgat ataacgtcca tttgtagctg
      841 tatagctacc acctgattct ttttgagcga tccattcttt ggcttctgct tcagatccgc
      901 ttacagttga aacaactgtt tcacttacta ctggagtttc tgctactgga gctgaaacag
      961 tttcatcttg agcggctggt gccgcataag tagctggcgc tggtgttgca acaggcgcta
     1021 cagggccatc gataaccaac tcttgatcaa cataaatcaa atgaatgtta tcaatgtggt
     1081 tgttttctgc caatttttca actgttgtgt tgtgagtttc agcgatttct gaaagtgtat
     1141 caccttcttt aacagtgtaa gttgatgatt cttgagcaga tacaaatgat ggagcaaata
     1201 ctgcaaacaa ggcagctact cctgcaagag ttgctttaat ctttttagtt attgatttca
     1261 tattcgaaaa ttctccttct ttctatagtt ctatcatacc ctttaaatat taccgtttct
     1321 tgacactttt atgtagaaat attacaaaat tattttttat ttccctaaat aaggtgtttt
     1381 ttgtcataaa agatactatt tttatgctat ttgaggtatt agaaaggttt tcatccacaa
     1441 ttaaagccaa gcccctcatt ctttgatata atagagataa gaatttttat aaggggaaac
     1501 tgatgaaatc acttcgtttt caatctgtct ttgatatcat cggaccagtt atgattggcc
     1561 catctagtag ccataccgct ggtgctgttc gtattgggaa gattgtctct tccatttttg
     1621 atgatactcc gacagaagtt gaattccaac tttttaactc atttgccaag acctatcgtg
     1681 gtcacggaac agacctagcc cttgttgcag gtattttggg aatggataca gatgatcctg
     1741 aaattccaaa tagtctggaa attgcccaca agcgtggtat caagattgtc tggaccattc
     1801 agaaagacag caatgcccct caccctaaca ccactaaaat tactgttaaa aatgcccaca
     1861 agaccatcag cgtgactggt atttctatcg gtggaggaaa tattcaggta accgaactca
     1921 atggttttgc cgtctctctc aatatgaata caccgactat catcatcgtt catcaagata
     1981 ttccaggtat gattgccctt gttacagagg cgctttcccg ctatggtatc aatatcgccc
     2041 agatgaatgt cactcgtgaa aaagctggtg aaaaagccat tatgattatc gaagttgaca
     2101 gtcgcaactg tgatgatgct atcgaagaaa ttcgaaaaat ccctcatctc cacaatgtca
     2161 atttctttaa ataggaggaa gcatgtttta ttctatcaaa gaattggtcg agcaagcaga
     2221 tctggacttt caaggaaatg tcgcagaact catgattaca acagagtttg aattgaccgg
     2281 tcgcgaacgt gaagaagtct tccttctcat ggaacgcaat ctggaagtta tgaaagcctc
     2341 tgtccaactt ggcctcaatg aaaataaatc tcgtagtggc ctgacaggtg gagatgctgc
     2401 caaattggat cactacatca aaaacgaaaa aactttatca gattacacga ttctctctgc
     2461 tgcccgaaat gctatcgcag tcaatgaaca caatgctaaa atgggcttgg tctgcgctac
     2521 tcctaccgca ggaagtgccg gttgtctccc ttccgttctc actgctgcta ttgaaaaatt
     2581 agacctcagc cacgagcaac aattggattt cctctttgct gctggtgcct ttggactagt
     2641 catcgcaaac aatgcctcca tctcaggtgc tgagggtggg tgtcaagctg aagttggttc
     2701 agcctctgct atgagtgctg ccgccttgac tctggctgca ggtggaacac cttatcaggc
     2761 cagtcaagct attgcctttg tcattaaaaa tatgctaggc ctcatctgtg accctgttgc
     2821 aggtttggtc gaagttccct gtgtcaaacg taatgccatg ggagctagct ttgctttcat
     2881 cgcagcagac atggccttgg caggtatcga atctaaaatc cctgtggatg aagtgatcga
     2941 tgccatgtac caagtaggag caagcatgcc aactgccttt cgtgaaacag ctgaaggtgg
     3001 actcgctacc acccctactg gtcgtcgcct ccaaaaagag attttcggag aataagctta
     3061 tcaaatagga gaaaccatga cctctatcac agcgattttt ttcgatttgg atggaaccct
     3121 cgttgatagt tctatcggga ttcacaatgc ctttacctat acctttaaag agctgggggt
     3181 gcctagccct gatgccaaaa ctattcgtgg ttttatggga ccacctctcg aaagtagttt
     3241 tgcgacctgc ctgtccaaag accaaatttc tgaagccatg cagatatatc gttcttacta
     3301 taaggcaaaa ggcatctatg aagctcaact ctttcctcag attatagact tgcttgagga
     3361 gttatcgagc agttatccac tctacatcac cacgacaaag aatacatcta ctgctcaaga
     3421 catggccaaa aacttggaaa tccatcattt ctttgatggt atttatggtt ctagccctga
     3481 agcaccccat aaggcagatg tcattcacca agccttacag acacatcaac tagcaccaga
     3541 acaagccatc atcatcggag ataccaagtt tgatatgctg ggagctcgag aaacaggtat
     3601 tcagaaattg gccgtcactt ggggatttgg agagcaagca gatctactaa actatcaacc
     3661 tgattatatc gctcacaaac ccttagaagt tttggcatat tttcaatagc atagactggg
     3721 taacaattcc atttcagaag aaaatgaagt aatactcaat gaaaatcaaa gagcaaacta
     3781 ggaaactagc tgcgggctgc tctaaacact gttttaaggt tgtggataga actgacgaag
     3841 tcagtaacca tatacggcaa ggcgacgttg acgtggtttg aagagatttt cgaagagtat
     3901 aagtctctac ataataaaac gcatattacc aaggttgtgt aacttgatac tatgcgtttt
     3961 tcttttttat tcaatgtggg ttgtttgatt agcaaaggcg ataatagctt gcttcaactc
     4021 atctccactg agagtgcgga actctccaat cttttgattg atggattcta aaggcatgac
     4081 attaacctta ccaacgaaaa tcttatccat aacttgatta tcaacaagtt cggttgatac
     4141 caagagttct tcgtagagtt tttcacctgg gcggattcca acttcaacga ttggaatttc
     4201 actttcagtg tggccactta gaagcaccat cttcttggcc aagtcataaa tcttgactgg
     4261 tttgcccata tcaaggataa agacttcccc atctttggca taagcaccag catggataac
     4321 cagacggcta gcttctggaa tggtcataaa gtaacgggtc atgcggaagt ctgtcaccgt
     4381 tacaggccca ccttcagcaa tctgacgttc aaagactgga atgacactac cacggctacc
     4441 aagaacattc ccaaaacgaa ctgcacagta ggttgattgg ctacgttggt taaagccagt
     4501 gacaatcaac tccgccacgc gcttggttgc tcccataaca tttggtggat taactgcctt
     4561 atctgtcgaa atcataacca tcttagacac tttagcttca tcaacagcct tagcaacatt
     4621 gtaagttcca cggatattgt ttttgaaggc ttcttttgga ttgcgctcca tcataggaac
     4681 atgcttgtgg gctgccgcat gataaacaat agcaggtttg tactgctcaa agacttgcaa
     4741 caaacgatca tagtcttgaa tgtccgcaat cacaggtaca taatcaatcc cttggaactt
     4801 acgaatcaat tcatgataaa caaggtagat tgagttttcc ccatgaccga gcaagacaat
     4861 gcgttcagga ttgaagcgac taacttgacg acagatttca gaaccgattg aacctccagc
     4921 tcctgtgact aagatggtct tacctgtcag ttctgcaccc agacgcgatt cgtcaagacg
     4981 gatttcctga cgacccaaaa ggtccgtaat atcaattttt tggaagccag tacctgcttg
     5041 gtgaaggccc tgaacaacag tttcaacctt aggcatcttg taacatttga cacccagctt
     5101 attacacatc tgcaagatac gctcatattc tgacggatcc agcgacggaa tcgcaacgat
     5161 gacacgctcg atttgatggc gtttggctaa ttcaggcaga ttgtcataag agcccaaaac
     5221 aggaatacca ccaagttttt gacccttttt cttagaatcc ttatccaaaa taccgaccag
     5281 ttctaattca ctggttggat gttggtaact atccataaag agagccccac catcaccggc
     5341 accaatcaag aaggtccgac ggtgttctcc atcaccacta ccttttttgc gtctggagta
     5401 gattaactgc caagtaatcc gtggcaataa aatcaagaag gtactcaaca agataaagag
     5461 aatgatgaaa cggatggaga agagtggcaa gaaggcataa cagatactat atgacaagac
     5521 actgctagca gtcacaccaa aaaagatttt catgaaatcc gtaatcttgc tgtaacgact
     5581 aatgctcgcg ttcaatcccc aaaaaccaat catcaattga tagaacagga aggccaaact
     5641 cgtatagata atgtagtcaa caggtgctgg attaatcagc ccataaaata aaatataaga
     5701 tacaattatg gaaaccacca tactgaaaat atcaaatatt ccccagaaaa cctgcttttg
     5761 ttgtttattt aaaatttcca ccagatcaat cacataatct gttagttttt tattcataga
     5821 aatctactcc tccttaaaag agttttacta tattgttatt gtagcatatt ttgatttatt
     5881 tggcttattt tatccaaata aattattgtt cccttgtact ccttcttcca tttaaatcct
     5941 ctaattaaac ctacaatcgt cccaaagcca taagcaaagt gaatggaaaa taaaagaaag
     6001 ggcatcacaa ttagaaatcc atttttatgt tttaataaag tcagcaaagt gagtagtgac
     6061 aaaagtagaa aataggcacc taatagtaaa gttatgaata cgaatgtgat cggtaacaat
     6121 gctagactaa acacaagact caaaacaaat aaacaaggaa catagtgaaa taatgataaa
     6181 cacttaaact gaacatgact tgtcaagcca atccacaaac catttaaata cttttgatgc
     6241 agcattttct tgaatgttgg tcgaatatac tgataagata gaatacttgg gctatagcgg
     6301 attttatatc catattctcg aattctataa tgaatatcat tatcttcagt tcggccaagt
     6361 tgctcattta ctaaaccaac cttctggaaa acctctcgtt tatacattcc atgaaaaata
     6421 gaagaaacat atctatcctc agaactattt cgataattgg caatgctact gccaaacata
     6481 ttttcctcaa caagatgcaa ggtctctgcc cattttcctt ttccttcgac aatcgtcggt
     6541 ctaggccccc cacagacaaa ttcaccttgt tgaataatag ccacattgtt cattacaaaa
     6601 gtctcagtaa cttttgaatg agcatcaatt tttaaaataa ggtcccctac agaatgttta
     6661 actcccaggt taaaaccact agcttgattt ttcttaggat tgttatacaa tctaattgag
     6721 ttaaactctg tatcttcctt tataaattgc tgaatgatag ctgtggtccc atctgtggac
     6781 atagcattta taaatagaat ttcaatattc tctttaggat aggtttgatt ttttaagtct
     6841 tcaattagac caggcagata tttttcttca ttgtatgcac tgattacaat ggatacgaga
     6901 agactttcaa cattttgagt cactactact ctcctgaatt tttccttcca ccattctatc
     6961 ataattttca agactttccc atttttatct tgaaagccag aaaccttact tgacattata
     7021 gtgaaaaagc cttaaaataa gctgttatta aaatcagcta gaagaggtat tatatgaaaa
     7081 agcaatcact cttttttgtt ccaggtatta tcctgattgg tgtttccttg cgaactcctt
     7141 ttactgtttt acccattatt ttgggaaata tttcgcaagg tctggaggta gaagttagtt
     7201 cgcttggtgt cttgaccagc ctgcctctcc ttatgtttac cctcttttca ccattttcta
     7261 cccaactggc tcagaaaatc ggcttggagc atctcttcac ctacagcctc ttcttcttga
     7321 ccatcggctc acttattcga ctaatcaatc tgcccctgct ctatctagga accttgatgg
     7381 ttggggcaag tgtcgcagtc atcaatgtcc tgcttcctag tcttatccaa gccaatcaac
     7441 caaagaaaat tggttttctg accaccttat atgtaacgtc tatggggatt gcaacggctc
     7501 tggcttccta tctagctgtg cccattacac aagccagttc ttggaaagga cttatccttc
     7561 tcctcacgtt actctgtcta gcaacttttt tggtctggct cccaaatcac cgctataatc
     7621 atagactagc tccacaaacc aaacaaaaaa gtcaaataaa ggtcatgcgt aataaacagg
     7681 tttgggcaat tattatcttt tcaggttttc aatccttgat cttttacacc gtcatgacct
     7741 ggttacctac catgtctatc catgcaggtc tatccagtca cgaagctggc ttgctgactt
     7801 ctatcttatc tctgattagc attccttttt caatgaccat cccaagcctg acaaccagtt
     7861 tatctactcg caaccgtcag ctcatgctca ctctggtttc actagctggt gtggtcggca
     7921 tttccatgct ctttttccca atcaataatt tcatttactg gcttgccatc catctcctca
     7981 tcggaaccgc aaccagtgcc ctcttccctt atctcatggt caacttttca ctcaagacaa
     8041 gcgcccctga aaagacagcc caattgtccg gcctatctca aacaggaggc tatatcctag
     8101 cagcctttgg gccaaccctc tttggttaca gttttgacct gttccactct tgggtaccat
     8161 ctgtagctgc cctcttgctc atcgatatcc tgatgactgt ggccctcttt acagtggaca
     8221 gagcggataa gatcctttaa acttctagtt ttagctagaa gttttttata tataaaaatt
     8281 ttacacattt ctcttgacag ggctttcttt tagatgtaca atgtatgtgt agaaaaatta
     8341 tatataaaaa tcttacacat tagaaaagga ggtttcccat gtactttcca acatcctctg
     8401 ccttgattga atttctcatc ttggctgtac tggagcaggg tgattcttat ggttatgaga
     8461 ttagccaaac cattaagctg atcgctaata tcaaagaatc cacactctat cccattctca
     8521 aaaaattgga aggcaatagc tttctgacaa cctattctag agagttccaa ggtcgcatgc
     8581 gcaaatacta ctccttgaca aacggtggta tagagcagct cttgacccta aaagatgaat
     8641 gggcactcta tacagacacc atcaatggca tcatagaagg gagtatccgc catgacaaga
     8701 actgaatacc tgactcagct agaactctat ctcaagaaac tacctgaagc tgaccgtatc
     8761 gaagccatgg actatttcag agagctcttt gacgatgctg gagtcgaagg agaagaagaa
     8821 ctcatcgcta gtttgggaac tcccaaagaa gcggcccacg aagttctatc caatcttctc
     8881 gataaaaaat caatgaagca cccgctcaaa aaaataaccg acaaatttta catatcgcct
     8941 tgttagccct ccttgcagca cctatcggca ttcctctggg aattgccatc ctcgtgaccc
     9001 tgttcgcaat tcttgtagcc gctttgactg tcattctggc tttctttgca gtttccatac
     9061 tgggtatcat cggcggattc ctatttttag ttgaaagttt cactgtcctc gcccaagcca
     9121 aatcagcctt tatcttgatt tttggttctg gtttactggc tatcggtgct tcttcgctag
     9181 ttttacttgg catttcctat gtaactcgct tcttcggtct actcattgtt cgtctggtac
     9241 aatttgttct taaaaaagga aagagaggta atcagcatgc gtaaatggac aaaaggattt
     9301 ctcatctttg gtgtggcgac taccgttatc ggctttatcc tgctttttgt aggtatccaa
     9361 tctgacggga ttaagagcct actttccatg tccaaagaac ctgtctatga tagccgtacg
     9421 gaaaagctaa cctttggcaa ggaagtcgaa aacctagaaa ttactctcca ccaacacgcg
     9481 ctcaccatca cagactcttt cgatgatcaa atccacattt cttaccatcc atctctttct
     9541 gctaaccatg atcttatcac aaatcaaaac gatagaactc tgagtctcac tgataagaaa
     9601 ctgtctgaaa ctccgtttct ctcttctgga attggtggga ttcttcatat cgcaagtagc
     9661 tactctagtc gttttgaaga agttattctc cagctaccaa aagggagaac tctaaaaggg
     9721 atcaacatct cagccaatcg cggacaaacc accatcataa atgctagcct tgaaaatgcg
     9781 accctcaata caaacagcta tatcctccga attgaaggaa gtcgtatcaa aaacagtaaa
     9841 ctcacaacgc ccaatatcgt taatatcttt gatacagttc ttacagatag tcagctagag
     9901 tcaacagaga atcacttcca cgctgaaaat atccaagtcc atggcaaggt tgaactgact
     9961 gccaaagatt atctcagaat catcctagac cagaaagaaa gccaacgaat taactgggac
    10021 atctcaagca actatggttc tatcttccaa ttcacaagag aaaagcctga atcaagaggt
    10081 acggaattaa gcaaccctta caaaactgaa aaaaccgatg tcaaggatca actcattgcg
    10141 agatctgatg ataatattga tctaatatcc acaccaagca gacgttgaca aaaacgctta
    10201 catttgctac tatggaaaca tatttatcta actaaaaagg aggttctacc atgaaacaag
    10261 aatggtttga aagtaatgat tttgtaaaaa caacaagcaa gaacaagcct gaagagcgag
    10321 ctcaagaggt tgcagacaag gctgaagaaa cgatagccaa tctcgataca ccaattggaa
    10381 aaaatactca agtagaggag gaagtctctc aagctgaagt cgaactggaa agccaacaag
    10441 aagagaaaat tgaagctcct gaagacagtg aagcgagaac aggaatagaa gaaaagaagg
    10501 catctaattc tactgaagaa gagccagacc tttctaaaga aacagaaaaa gtcactatag
    10561 ctgaagagag ccaagaagct cttcctcagc aaaaagcaac cacgaaagag ccacttctta
    10621 tcagtaaatc tttagaaagt ccttatatcc ccgaccaagc tccaaaatct agggataaat
    10681 ggaaagagca agtgcttgat ttttggtctt ggctagtgga agcgatcaaa tctcctacaa
    10741 gtaagttgga aacaagtatc acacacagtt acacagcctt tctcttgctc attctgtttt
    10801 ctgcatcttc ctttttcttt agtatctatc acatcaaaca tgcttactat ggacatatag
    10861 caagcattaa cagtcgcttc cctgagcagc tagctccttt aactcttttt tctatcgtct
    10921 ctatcctagt agcgacaaca ctcttcttct tttcattcct cttgggtagt ttcgttgtga
    10981 gacgatttat ccaccaggaa aaggactgga cgctagacaa ggttctccaa caatatagtc
    11041 aactcttggc aattccaatc ttcctcactg ctattgctag tttctttgct ttctttgata
    11101 gcctacgatt tacagccctc ttgtgtgtga ttagcattgg aatcattctg cttgccagtc
    11161 tccatatcat tacaagacca agtcaagcaa gtgaaaccga ctccttctat caattattct
    11221 tgtctgtcct tgtgaacgga gttattatcc tcctcttctt tgtagctgaa gtggcactga
    11281 ttggagatta tcttcgtatc ttggcctttc tttaaaattc aatcctgtca ttcttggcag
    11341 gattttttct atgtcaaaaa agcaagtcga ttgacctgct tctgctttac ttttcttatg
    11401 ctaatgtttt aaaatcttgt actaggatgg gttgtatttc tatttgattg gcgtctagct
    11461 ggtggtaaaa agatgggggt aacacctcta agaattttgc atagtccaag cgagctatgg
    11521 attcatagtc ttctggttta gagccgtctc ctgtgatttg aaccaagtca atctcccact
    11581 ggacttcctt atccaccaat tgctcaatat aggctgcagg aacaaacggt tctctcggta
    11641 aaactgtctt gactccttga gctagatggt aaacgccatg cacttttcct tctccatcct
    11701 gatagaagga aactctcgca aagtaggcat ctgtctcttg aaccgcacgc acacgcgctt
    11761 caaactgagc caagccatct gcctctaaaa agcttttcaa gttctcacga ctaaagaaaa
    11821 taggattaaa aattccttgg caaatcgtaa aacgagctac agggtctgct agataggctt
    11881 gaatacttgc ttggaaataa gcttcaaaat caaaaccatc tagtccttca atatcagtcc
    11941 ctgtataagc aagcaaagca tctaaaaatg acttgataat ctgccagtct gtctttgtta
    12001 gtaggtcagg aacatcgaca cgataagcct tttgaccatc agcataactg accttaaaga
    12061 gaaattgtga ttgccccact atcgcacact cgatatattc gagacgatta agaggctgac
    12121 ggagatagac tacatcatag ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact
    12181 tggcattcaa aatctcttgt tctcctaaaa tgctctgttt atttggaata aaaaatgttt
    12241 tcgccataac tggactcctt tgaaatcgtt tacatacagt ataccttatt tttctgaaag
    12301 atacaaaaac aaactttaga tttttgagta caaagcatag aaaaacagcc cctgaggact
    12361 gtttgatctt atacagtagc tgcttgatcc aagctttcac cgatagcggc taggcgctcg
    12421 atcacttcag cttgtgtcaa ttcatttttt gaaacatagc ggttacgtgg gtgaacacgg
    12481 cactcgtgtg agcatccacg aaggtacttg tcttcatttt cttctgatgt caagatacga
    12541 cggttacaga agggatttcc acagttgaca taacgttcac atggtgttcc atcaaaccag
    12601 tctttcccta cgatagttgg gttgacatgg ttgacatcaa cggcaatacg ctcgtcaaag
    12661 acgtacattt tcccatccca aagctcacct tgaacttctg ggtctttacc gtaagttgcg
    12721 attcctccgt gcaattggcc gacatctttg taaccttcac gaaccatcca gcctgagaat
    12781 ttctcacagc gaacgccacc tgtacagtaa accacgacac gcttgtccat gaatttttcc
    12841 ttgttatcac ggacccattg tggtaactca cggaagttgc gaatatctgg gcgaatagcc
    12901 ccacggaaat gtcctaggtc atactcataa tcattacgtg tgtcaaggac aacggtatct
    12961 ttatcaagaa gcgcttcttt gaactctttt ggagacaagt aagcacctgt tgtttcaagt
    13021 gggttgatgt cattgtcaaa gtcgttgtct tccaaaccaa ggtggacaat ttctttcttg
    13081 tagcgaacaa acatcttctt gaaggcttgt tcactttctt cgtcaatctt gaaccagagt
    13141 tcttccattc ctggaaggct gtgaacgtag tccatgtatt tttgagttgt ttcatagtca
    13201 cctgaaactg ttccgttaat tccctcgtca gcgactagga tacgtccttt aagaccgatt
    13261 gatttacaga aagccaagtg gtctgcagca aattgctctg cattttcaat tggagtataa
    13321 aggtagtaaa gtaagacacg aatatctttt gccataagat ttgttctctt ttctattctt
    13381 aaattttcta aattttttat ttatctacac tagtatacct gcttgagaaa acgaatgcaa
    13441 tttatttgac tggaaattgt agaggggtct cgaaatttcc tgaatggtta aaataaggac
    13501 aagagaaaac atggatatct atatccttgt gccaaaaaaa ccactgtctc ccccagacca
    13561 acctgaggaa agcagtgatt cttattttag gagttaggaa tgaatacacg aaatcaattt
    13621 agctgattat tttttgtttt tcaagaattc atcgtattgt ttttgcattt cgttcaatac
    13681 tttttcgtag gcaccttcag atttcaattt ttccatcaat tctggaatcg ctttatctgg
    13741 gtctacagta ccagtgttga tagctgtatc aaattgttgc attgtgttag caatagctga
    13801 gatttcagat ttcacattgt cagtattgaa gataaatcca agcgctggag attctttagc
    13861 ttctgccaat tctttcttag aattttcgat ttgttggtct gtaacgtttt cgttgatgta
    13921 aaggatccag ttgttaccag tgttccatcc acccatgtga gtgtttcctt tgtagccatc
    13981 aagaacgcga acacggtttt ctttaccttc aattttttcc cagttcttgc cttctggacc
    14041 gtaaacaaga ccgttcaaga gttctgggtt cgtattcaag aggttcaaga tttccattga
    14101 tttttctttg ttcttagagt tgtttgagat gacaaagtta gcaacttgtg ttgtttggtt
    14161 tttcttgatg aagttagtaa ttggtttgat ttggatatct ttgttggcaa cacgtgaaag
    14221 caagctgtta ccgtagtcag ctggtcctac tgtttcttca cgaacgaacc aagtatcttg
    14281 ttgaaggtca aatgaagtgt cacttgttgc gacgtctttt ggaatgtagc cagcttcata
    14341 gaatttgtga agagtcttca agtgttcttt gaaacgaggc acttcgtaac ggtttacaac
    14401 tttagtagta tcgccttcaa ggtcaataac gaatggaagc ccgtttgcta ctgggtagtc
    14461 aaagttatca gatgggatga aaactttacc aatagcaaat ggtactacgt ctggagcttt
    14521 ttctttgatt tgtttcaaga ctggctcaag agtttcgtaa gaagtaacac ctgaaatatc
    14581 gataccatat ttggcaagga gagttccgtt gaaggtaaag ttttgagatg atgctacgtt
    14641 ggctgcaact ggtacagcgt aaatcttacc atttacagta ttacccttga tataagctgg
    14701 atcaagtgcc ttgtaaaggt ctttaccttc ttttttgtac aattctgtca agtcagcata
    14761 agcacctttt tgagcattta caatatagtt atctgcaaag gcgatatcat agttttcacc
    14821 agatgatgtg ataactgaca ttttcttacc atagtcaccc cagccaaggt attggatatc
    14881 caatttggca ccaacttttt cttcgatgat tttgttggca tttgctaaca attcatccaa
    14941 gttatctggt ttgtcaccga tttggtacat tttgataaca gatttgtcac ctgaatcagc
    15001 agcttttttg ctgttacctg tcaagttgcc acaagcagca agacctgcag ccaaagcgac
    15061 tacactagca gatgcaaaag catatttttt ccaatttttc atgataaaaa ctcctttttt
    15121 tatttttaaa cttataaaca atgtaatgat cttaacttca cgcaagggaa gtttggaaat
    15181 gagaaatgtt ctttttcaat aagcactatt ctttcacacc accgatagtc aaacctttta
    15241 caaagtagcg ttggaagaat ggatacaaaa tcgcgattgg aagggttgct accacaacca
    15301 tggccatacg acctgtttct ttcggtagag caactcccag ttgaccagtc aagccgaccg
    15361 ctttggcaat gtagtccata ttttgttgga tttgcatgag caaatattgc aatggataca
    15421 agttatcact cttgatgtaa agaagggcgt tgaaccagtc attccagaaa ccaagagctg
    15481 tcaaaagcgt gatggttgcg atacctggta gtgacaatgg caaacagatt tggaagaaaa
    15541 tccgagcctc actggcacca tcgatacgag ccgattcgag aatggcttct ggaatggtct
    15601 tcttgaaaaa agaacgcatc aagatgatat tgaatggtga gagaagcatt ggaacaatca
    15661 aagcccaaac agtgtcacca agctgaagta cacgggtcac catgatataa cctggtacca
    15721 aaccagcgtt gaacaacata ctgagaagga cgaagatggt aaaaaatctg cgatacttaa
    15781 aggttgtccg tgaaatagcg taggcatagg ttgttgtgat aaagacattt gtcaatgtcc
    15841 caactacggt tacaaagaca gagatgaaga gggcttgtag gattttatcc ttaaactgtg
    15901 ccaaaaactc aaaaccgtct aagccaaatt gggatgggaa gaagctatag ccgtattgga
    15961 ggaggctttt ctcgtctgtc actgaaataa tgataacgaa tacaaaaggt aggatacaag
    16021 agagggcaat caaacccgaa atgatactga agaagatatc tgctttctta ctgaaggagt
    16081 gaatgccgac attatcaatt ttttcttttt taattttctt ttttgccata ttctcctcct
    16141 ttctagaaca aagctgagtt tggatcgact cgtcttgcaa gcaagtttga taggataacc
    16201 agaatcaaac caacaacgga ttggtaaaga ccggctgctg cagccatacc gatatctgct
    16261 gtctgagtca aaccattaaa gacatatacg tccaaaacgt tggttacatt gtaaagctga
    16321 ccagcattgt gtgggatttg atagaagaga ccgaagtctg cgcggaagat atttccgact
    16381 gcaaggatgg tcaatacagt tacaagcgga gtcaactgag gaatggttac attgcgaata
    16441 cgttgccact tgctagctcc gtccactgtc gctgcttcgt agtaggttgg atcaattccc
    16501 atgatcgtcg catagtacat gacactgcta tatccaaagc ctttccaaat acctaggaaa
    16561 agtaggagat agggccagat gcccaggtca gcgtagaaat tgacttcttt gagaccaaga
    16621 ctttccaata gatgattgaa caccccttta tcaatattta ggaaggcatc tgtaaagaaa
    16681 ctgatgataa cccaagacaa gaagtaaggg aacaacatag aagtttgaaa aatcttcacc
    16741 attctcttag aacggagctc gctgaggata atggcaatcc ctacagatac aactaaacct
    16801 agaaagataa agccaagatt gtagaggaca gtatttcgtg tgataataaa ggcgtctctt
    16861 gaactaaata agaatctgaa attatcgagt ccgacccatt tactatttat gatactatct
    16921 atgaaaccat tactggtcat gtggtagtct ttgaaggcaa ccacgttccc aaatactgga
    16981 atgtaaaaga atagaatcaa ccagagtgcc cctggcaaaa ccatcaagag aaagatccag
    17041 ttgtctctca atgtttttga aaactttttc ataatttcct ccctttttat tttgatatcc
    17101 atctaaaaat tcttttttag acttttgata acgattacat tattagtata ctcctatttg
    17161 caggttaggt taaactccta attatagaaa aaactccaca aattatgtag cagatttaaa
    17221 actttatcac cactatcaaa caaatgtcct aaatcaattg tttattttat ctctattagc
    17281 ccagtgatgg cgtcactctg ttataagcat ccaacaacgg ggtatactga aaaatctcca
    17341 gactagggaa ctcagcgata gttcctaatc tggagatttt taatatgtta ttaggcgttt
    17401 gctttcaact tagcaataac ctctttaaga ttatcaatca actctgctgc agtatgctca
    17461 gagccttttt catctgccaa gaacaaaact gctttttgaa gttcttcttg agagttttca
    17521 aggacatcct tatctactgt ttcaaggttt gagtctttaa gaagtttact taattccttg
    17581 gctaatttct tgagtttgat ttgcagactc atcttctcct gctgtttctt tgcccgctgt
    17641 ttgtcctcca tccttagttg ctgactggct ttccttaatg gactctaggg aagcaatggc
    17701 atctttgact gtttgcaaga tatcacgtaa accttgctct atcaaactat catctgcaaa
    17761 agctttctta gagtctgcca aaaccagacg tgctgaatct gtggtaggat tcgatacacc
    17821 tgtcaatgat ctcaaaagat tttctaaggt ttgagtctgc ttactaatac tagactaaaa
    17881 tcaaaaagca ttatataaca gtgatatgaa atcaactaaa gaagaaatcc aaaccatcaa
    17941 aacactttta aaagactctc gtacagctaa atatcataaa cgccttcaaa tcgttctatt
    18001 tcgtctgatg ggcaaatctt ataaagagat tatagagctt ttatagtgat ttgaaataag
    18061 atgtgaacaa ctctatcagg aaagccaaat taatttatag aaatatttta gcagccaagg
    18121 tgtactgtta tagattcaat acactatact tggtggttta gctctcctgt tttctctttt
    18181 agctttaacc agccataaat ggtattacgt gagatttgga aaacgtgtga tgcttctgtt
    18241 atactacctg ttcgctcaca ataagagaga acttttttac gaaaatctat tgaatattcc
    18301 ataaaagatt ataccacatt gtgtactatt tttggttcat tttactatat cactttggac
    18361 tatatcggtc tagttatgtc taaacgatta tagctggata ctaagagaaa tcaaaaaagc
    18421 actaagggag aacgtcccaa agtgcttaat atcaaggcct caaagcctgt cttattcaat
    18481 aaaatattac aacatcttgt tgtagaattc aacgacaagt gcttcgttga tttctgggtt
    18541 gatttcgtcg cgttctggca agcgagtcaa tgaaccttcc aatttttcag cgtcgaatga
    18601 tacgaatgct ggacgtccaa gagtagcttc tactgcttca aggattgctg gaactttcaa
    18661 tgatttttca cgaactgaga tcacttgacc tggagttacg cggtatgatg ggatatcaac
    18721 gcgtttcccg tcaacaagga tgtgaccgtg gtttacgaat tgacgagctt gacgacgagt
    18781 agtcgcgaga ccaagacggt aaacaacgtt atccaaacga cgttccaaaa gaagcataaa
    18841 gttgaaacct aggattccgc ctttgatttt tgtagcttgt acgaacaagt tacggaattg
    18901 tttttcacct acaccgtaag tgaaacgaag tttttgtttt tcagccaatt gcaaaccgta
    18961 ttctgacaat ttagaacggt tgtttggtcc gtgttgtcct ggtacgtagt tacgacgtgc
    19021 caattcttta cctgtacctg taagtgaaag gccaaggcga cgagcttgtt tccaagatgg
    19081 tcctgtataa cgtgacatat gaatgtcctc ctgatataaa taatatttcg gtggaaatag
    19141 tcacttagaa agccctgatt cgtgcagatg cccttcgcct aaacagccaa ggttacttgt
    19201 cataagacac ctgttgacga gcttcatgct ttcctgctga tatttcacac aaaatctatt
    19261 ctaccatgaa tagctggatt tgtaaagggg ttttaggctt tattttcact accagagagg
    19321 ttgagaacga gggtaaaggt acttcctaga ccgtactggc tgctgactgt gatttcccca
    19381 cccaattgat gggccaattc acgcgcaatc gcaagtccta atccatgacc acctgtcttc
    19441 atgttacgcg aagtttcgac acgataaagg cgtttgaaaa tattttccaa atcctctggg
    19501 gcaataccct gcccttcatc ggtcacactg attgaaagct ggtccttctc cagcttagcc
    19561 accacttcca gcttggttcc tggagcagaa tatttaaaag cgttatcgac cagattcacc
    19621 aagatacgag aaagcttagc ataatctccc tcaatccggg cagactctgg gattacctgc
    19681 aagtggacat ctcttctctc ctgctcaatc aaaaactgaa attcactcat gcactcaatt
    19741 aagagcttgt ccagaaaaat actgtcttta ctggtagttt ccacctgatt tctagctgtg
    19801 tttagggtca aaaaattcaa ctcctcaacc agtttattga gcctctccgt ctggcgtcca
    19861 atggttgcta gataatgagc ttgctccgac tccttgataa taccatccaa aatcccttct
    19921 accgtcgctt ggatcgaagt gataggagtc ttaatatcat gcgacaactg ggcaatcatc
    19981 aagccctttt ctcgttcgct ttcttccaag gaatcaaagc ttacctgcaa atcatgggac
    20041 atctcattaa aagtttgccc taattgctga aattctacag gaccttgaac ctccaaattt
    20101 gaaggaaaat ccttggccgc tacccgcttg gcatgctcct tgagtttgcc caacgacgta
    20161 aagactggca ataggagaaa gagactaatc ccagcaccga caaggctggc aacgatggtc
    20221 atcccaagca aaaagtaaat ctcgcctttc gcaatcagca ttttttgaac agcccaaaaa
    20281 acaaccaaaa tggttaagag ggttgaaata atatatccaa ccaaaatata actttttagt
    20341 ttcatgtttg tcctctcggt ttctctatct tatatcccaa cccccaaact gtcttaatag
    20401 tgggcgtttg gtcactacta tattttgcca gctcctgtcg aagagcatgg atatgcacat
    20461 tcaaggtatt ggtgtcatcc acgtagtctt ctttccagat cttttcatag aggtctgtct
    20521 tggagaaaac tcgctctgga ttgctagcta aaatccacag caattcaaat gatttaacag
    20581 ttaaatccag catttcttct cctatttgaa cttcatgact actatgattc atttttagat
    20641 tgccaaggga aatcagctct gtttcgcccc cacgatgaag gcggcgcaaa atattgtgga
    20701 cacgcaaaac cagctcacgt gggctaaaag gcttagcaat aaaatcatct gctcccaagc
    20761 tcaggccgta aatcttgtcc tgttcactgg tcttagcagt aataaatagg aaaggctgct
    20821 ctggtgataa gtattgaacc tcactgatta aatcataacc atccatccga ggcatcatga
    20881 catctgtgat aatcaaatca atcggttttt tcttgaacag ctctagcgct tccagtccat
    20941 catgggctac caagacctga taacctgcct gaattaagta tctctgatga atatctgtga
    21001 tttctacctc gtcgtcaacg agtaaaattg tctttcccat ctgtctctcc tttgataaaa
    21061 acagtgctat actgctttaa gtataacact atttttattg ttttacgatg attttaatct
    21121 gctattgaaa gaaagtcctg attgctgatt gagttgttaa actagtctta gtcagcccac
    21181 tatccatcat ttatttcctt agctgattag ttttcttctt ttttgttttt caaacctaga
    21241 ccaccgagta aacctgcaag cgcaagccca aggaaaccaa tacttgccat tgatgtttga
    21301 gtctcaccag tatttggtag catagcttta tcctctgaca tcatcgtatc agacatcttg
    21361 ttagcagaag cagccatgtt ttcacctgcc atcgtgttgg tagaacttgt catggtgtca
    21421 gcaggcatgc tatctgtagt acctgtagca tgattgtgat tcatcggagt cacgccagaa
    21481 ccagagttag aaggtgataa tgaaccattt gctgtgtctg atgtttcttt aacatttat
//
LOCUS       DBERCA010000027        21106 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.027, whole genome shotgun sequence.
ACCESSION   DBERCA010000027 DBERCA010000000
VERSION     DBERCA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.027"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..294
                     /locus_tag="ACL719_001187"
     CDS             <1..294
                     /locus_tag="ACL719_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699156.1"
                     /translation="NYFVSLNYLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGS
                     FEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            609..1586
                     /locus_tag="ACL719_001188"
     CDS             609..1586
                     /locus_tag="ACL719_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699157.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            1963..2337
                     /locus_tag="ACL719_001189"
     CDS             1963..2337
                     /locus_tag="ACL719_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HGS0699158.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            2344..2526
                     /locus_tag="ACL719_001190"
     CDS             2344..2526
                     /locus_tag="ACL719_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699159.1"
                     /translation="MGIKMEKIFVIIFFVCLFISSITFLAYDFVSEEIKKLIIWINVV
                     FLILIIAMMIYPKLRK"
     gene            2545..3447
                     /locus_tag="ACL719_001191"
     CDS             2545..3447
                     /locus_tag="ACL719_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699160.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            3503..3856
                     /locus_tag="ACL719_001192"
     CDS             3503..3856
                     /locus_tag="ACL719_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS0699161.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            3853..4581
                     /locus_tag="ACL719_001193"
     CDS             3853..4581
                     /locus_tag="ACL719_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699162.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            5060..5266
                     /locus_tag="ACL719_001194"
     CDS             5060..5266
                     /locus_tag="ACL719_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699163.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFFYKE"
     gene            5682..6221
                     /locus_tag="ACL719_001195"
     CDS             5682..6221
                     /locus_tag="ACL719_001195"
                     /inference="COORDINATES: protein motif:HMM:NF037419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HGS0699164.1"
                     /translation="MLFYFEKIWSLSLKLLDCILDYQERFNGKTCQVSTNYKYLEIFK
                     VNFCLTDLHHLFGLHKITRDYASQTKPAIQTGVFILEDFRNILLYNDVIERISLYEFI
                     SDIFYSKITSCCIVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFT
                     SAKKYAKVRKTDVKEMKWL"
     gene            6506..8413
                     /gene="pspA"
                     /locus_tag="ACL719_001196"
     CDS             6506..8413
                     /gene="pspA"
                     /locus_tag="ACL719_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HGS0699165.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSSPTFVRAEEAPVASQSKAEK
                     DYDAAKKDAKNAKKAVEDAQKALDDAKAAQKKYDEDQKKTEKKAAAVKKIDEEHQAAN
                     LKSQQALVEFLAAQREGNPKKKKAAQAKLEEAEKAEKEKKKEFDKAQAVVVPEATELA
                     ETKKKADEAKVKEPELTKKLEEAKAKSEEAEKKATEAKQKVDAEHAKEVVPQAKIAEL
                     ENEVQKLEKDLKEIDESDSEDYVKEGLRAPLQSELDAKQAKLSKLEELSDKIDELDAE
                     IAKLEKNVEDFKNSNGEQAEQYRAAAEEDLAAKQAELEKTEADLKKAVNEPEKPAPAP
                     ETPAPEAPAEQPKPVPAPQPAPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRL
                     TQQQPPKAEKPAPAPKPEQPAPAPKIGWKQENGMWYFYNTDGSMATGWLQNNGSWYYL
                     NANGSMVTGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGAMATGWLQNNDSWY
                     YLNASGAMATGWAKVNGSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWVKVNGS
                     WYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVD
                     GYKVNANGEWV"
     gene            8814..9935
                     /gene="mnmA"
                     /locus_tag="ACL719_001197"
     CDS             8814..9935
                     /gene="mnmA"
                     /locus_tag="ACL719_001197"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGS0699166.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            10076..10531
                     /locus_tag="ACL719_001198"
     CDS             10076..10531
                     /locus_tag="ACL719_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0699167.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEYAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            10541..12454
                     /gene="mnmG"
                     /locus_tag="ACL719_001199"
     CDS             10541..12454
                     /gene="mnmG"
                     /locus_tag="ACL719_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGS0699168.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMINEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(12788..14467)
                     /locus_tag="ACL719_001200"
     CDS             complement(12788..14467)
                     /locus_tag="ACL719_001200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS0699169.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAIKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(14469..14702)
                     /locus_tag="ACL719_001201"
     CDS             complement(14469..14702)
                     /locus_tag="ACL719_001201"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGS0699170.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            14982..15197
                     /locus_tag="ACL719_001202"
                     /pseudo
     CDS             14982..15197
                     /locus_tag="ACL719_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15520..15672)
                     /gene="cibB"
                     /locus_tag="ACL719_001203"
     CDS             complement(15520..15672)
                     /gene="cibB"
                     /locus_tag="ACL719_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGS0699171.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(15675..15861)
                     /gene="cibA"
                     /locus_tag="ACL719_001204"
                     /pseudo
     CDS             complement(15675..15861)
                     /gene="cibA"
                     /locus_tag="ACL719_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
     gene            16038..16721
                     /gene="tsaB"
                     /locus_tag="ACL719_001205"
     CDS             16038..16721
                     /gene="tsaB"
                     /locus_tag="ACL719_001205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGS0699172.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEAESLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            16718..17155
                     /gene="rimI"
                     /locus_tag="ACL719_001206"
     CDS             16718..17155
                     /gene="rimI"
                     /locus_tag="ACL719_001206"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGS0699173.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            17145..18155
                     /gene="tsaD"
                     /locus_tag="ACL719_001207"
     CDS             17145..18155
                     /gene="tsaD"
                     /locus_tag="ACL719_001207"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGS0699174.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGESYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(18195..19500)
                     /locus_tag="ACL719_001208"
                     /pseudo
     CDS             complement(18195..19500)
                     /locus_tag="ACL719_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(19836..20118)
                     /locus_tag="ACL719_001209"
                     /pseudo
     CDS             complement(19836..20118)
                     /locus_tag="ACL719_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20255..20545)
                     /locus_tag="ACL719_001210"
                     /pseudo
     CDS             complement(20255..20545)
                     /locus_tag="ACL719_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            20788..>21106
                     /locus_tag="ACL719_001211"
     CDS             20788..>21106
                     /locus_tag="ACL719_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HGS0699175.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKI"
BASE COUNT         6537 a         3682 c         4535 g         6352 t
ORIGIN      
        1 aattactttg taagtttaaa ttatttacta tataaggttg gatttttggg aatcatttac
       61 gataatgaag cattaagaga tttgtcaaaa caatatttaa acaaaaatat ttcggaatta
      121 caagatggtt ctttcgaggg cggtatacaa gatgctgttg cctctaaaca aatagtagtt
      181 ataaatgaat ttatagcttg tttaaattcc agatgcgtgc catcccaata tgatagattt
      241 ttttataaag atagacctta tattttttcc agaaagagtc ctattaaggg gtgaaaattt
      301 gttacatttg gattaacaag tgtcctaggg attagaaata actctagtat agctagatat
      361 ttttgagtaa ctagaaatat cattaggcta cccatatgat tgccagaaaa gatggctgat
      421 gaatcttatt tatactgttg cagtaggcat tgtctaggat aaataagcta tcttgatggg
      481 ggagattgga ataattttag tagaaacatt aaaggattat ggacaagtag cgcaggatta
      541 ggtgatgcaa tgtgctaagt tggcttggtg ttagcagtag tattatttaa caatggaaag
      601 tagactaaat gaaaaataaa cgttattttt ttgacacaat tttaataatt ttactgctta
      661 taagcacgat attttgtgtc agcccagtat ttataaaatt ggacatctta ggaactccct
      721 ctcatgccat cttaaccttt gtattggcga taccgttatt ctatattttg tctcaatgct
      781 tacatacttt gctattacta gtttcctcaa tcttttgtaa attaagacca atttattttt
      841 attttatatt tgtgatcatt ataggagcta ggaaatacta tcgaattctc tttcaccaac
      901 taatgggatt tagtccagga atagcagtgt tttataagga gagtcaaaca actaagaatc
      961 tgtttaaatt ttactacttc ttgtacttta caacactaat ttcatactat tttttcttta
     1021 cttttgtgta tgacaaaccg ttgcttttgc ctcttattcc attttctatt atcatagcat
     1081 tagtacaaaa actttataga attgagaatc aacaattatt tctgttaaaa tcaaaggtac
     1141 taacaatact ggaatctaag agggattgtg agtttaatct tcaggattat catgaaattt
     1201 ggaaattgca atcaaaatct gagttgccat gcgtggcttt atcttatatc agtttgatca
     1261 agccatatct ttcagagagc gtgcgtgaac aaattgattt attagaagtt aagcgattta
     1321 aaaaaataaa tcatccaata tcactttatg gtatgttgga tgttataaaa ttaaatctat
     1381 atttacggca ttataatgaa aaaaataaat atgaatcaat gttaaataaa attttagagg
     1441 taagaccaga ctttgtttta atcgagcaga atattgatga tagtttaaat agctcgcaac
     1501 cattatcttt atctttagcc atttcagaaa tccaactttt gcttgaagtg tatatgggga
     1561 tcaaacatgt ttcaattagg agataaaacc agaataatat ttgccattat aggggcaagt
     1621 tgcatattag tcagtatgta tcttattcca cttattaaaa ctgagaataa attttgggaa
     1681 atacttttat ttatattagg tgtattgcta attggaattg cgttattaag tcaaaaaaaa
     1741 taaattttga aatagtatta gtgagttcgc aatcatttat tgctagtggt ttagaactgt
     1801 taagtttcaa acaataattg attttaaacc ttaggtaacc tataaaaaca aataaggtag
     1861 agaaaggata tcttatctag gtagctaagt gataaaaaca cagtcgtcta tcacttgtta
     1921 ggaaactatc ctaactatgc ttaataaaaa aggagaaaat aaatggaact cgtattacca
     1981 aataattatg ttgttcttga gcaagaagag atgatgtatc ttgatggggg attttctatt
     2041 ccgagatggc ctgttgcaac agccattaat atagctttta atggtgtttt aggtggagga
     2101 gcaatcagtc tagttagaaa ttatattcgt aattatggtt tgcggcgagt tacaagcgca
     2161 attgctggag cagctgcaag atatgttggg gtacgagttg caaatagagt ggcaggattt
     2221 gcactgtctg ctattaatgg atttgcagct tggatgtcaa ttggcgatgc tattacaaca
     2281 atctgggcca ataatgatgt aaataggaga gacccaaatt taaacgcctt gtggtaacta
     2341 gatatgggta ttaaaatgga aaaaattttt gttattattt tttttgtatg tctatttata
     2401 tcatccataa cttttttagc ctatgatttt gttagcgaag aaataaaaaa gttgattatt
     2461 tggattaatg tcgttttttt gattttaatc atagcaatga tgatttatcc aaaattaaga
     2521 aaatgataag gacactgtaa aattatgata cgtaaactaa ttgttttctt actaatgctc
     2581 cctctttttg tcttgtggct agagttgcat gtcctagtga ataacttact attgaatcta
     2641 gaatctcccc ttgattttgt gattagtatg agtcttgctt tctgtagttt gattttatca
     2701 aaaatagttt tagacatact atacgcattg aaagatttat acaagaaaga aggtcttatt
     2761 aagatttttc cctttatttt tataagaagg aaaaaagtga atgtgagatt ctcgccctat
     2821 ttttcattcc atcgtaaaag tttgtcgcct gatgatttaa gatctaggat tatatggagt
     2881 tttattcttg aaattgctat tatcctagta tttattttaa aaattccttt tgcaatcatc
     2941 atgcttacta caatattttt ctggactata atggatatca atcatctcgt ttttaataag
     3001 acggaatttc tttttaatca aaacaaatgg caaaaagaag attcatttga aagtaatttg
     3061 cataagatat tgatggacaa aatacaaaaa tcagaattaa gctattctga cttaatgtcc
     3121 cttcttctct atgatgcgat gaaccaatcc acctttttaa cggatagtga actttttgag
     3181 gatattttga aaaaaatcga agattctcat aacacccttc tgtgtacagg tcttgtcgag
     3241 ttactgctgt atgagatttc tattagtaac aataacaact ggcaagaaaa ggttgataaa
     3301 attagaatac agctcatcag aataaatcaa ttagattttt tctattatac aagttggtta
     3361 aggcaaaatt ttgatttctg tatgaataga gagtatcata aaatgaaatc tcggaaatta
     3421 ttactgagta ataaaaagat agtttaagtt cgtcctattt gtaaaacaaa tgaaacaatt
     3481 tcaactaagg aggagaaaac aaatggaact tgtattacca aataattatg ttgtgattga
     3541 tgaagaagta atgatgtatc ttgatggggg agcttattta agcaagcgtg cttgtcaagg
     3601 aatttgcgca gctttagcta tgagttcagg aacttttata gcattagctg gagctgcagt
     3661 tttaaccaaa aaactaataa actatattaa ggttggaggt cttggaggct ggcttattgg
     3721 tgcagcagca ggtgtattgg ctacagcagc agggaaaata gcttactgta ttggatatgg
     3781 tgttcttaat agaggttgtg atattaacgg gaacccttat ccttgggatg gattcatatc
     3841 tgcaacagta agatgagtaa tgtagataag attcgaaaaa tacacatcat cgtgtgttgg
     3901 gtgtatatat ttttatcatt cagggcgatt ataaatgata cggagtattt tttgcttatt
     3961 tttttagctt ttatctactc aatagtatca ctacctctat attctgtgaa aaataaaata
     4021 gtatctatct gtctagccat aaattcgatt ctgttaatga gttttcccat tttaatcaat
     4081 aagtttttcc cagaaagttt tttgacttat accgtattaa taagtgtttt tattacagag
     4141 ttgataattt ttcatttaat tgggaaagat tttgatacta aattgactaa cgaatataag
     4201 aaaattagtc agttcagaag caaggtgtct caatctcctt ggataaaata tttagagatt
     4261 tctagtttta tattaactat atttccatcc attctttatg gtacagttga taatcatgta
     4321 ctgactctta tctttttgat aaaaatttgt gtagatacta cgataaaatt tttattcatc
     4381 agattatttg acacaagtac tttaatgaag aggagaatat tttttctttt cgcattggat
     4441 gttatagctt acttattttt aggatatctt ttagtgattc aaaaagcagg ctatttgttt
     4501 tcggttttac ttcttttttc taatttttca gttccattta tcaaagaaaa ggaattcgaa
     4561 ttatttaaaa atagcaagta atagaaacat aggttaaatc ttagaacggc ttaaaatcag
     4621 atatgataaa gggaaatagt cttatctagg taactaagtg ctacaaacac agtcgaggat
     4681 cacttgttag gattacattt taatcatgct taataagaat ggagaattcc tatggggatt
     4741 gtattactct gaagtttcga ataccttagt tctcgtgatt ttgagctatt ctaggtttag
     4801 aatccttcag aatgtaaaat tttaagcagt caaagactta gaatagcccg aaaacattta
     4861 ttttatagca attctaggct tagaattgct catatatatg tatttgagct gctcgatagt
     4921 taggacagct ttattctgag aaatctgagc tatcaatgct tcagaatagt atttcgccaa
     4981 tggtttttat tgaagaggtg attgttttat gatagtctaa aataaaactg atttaatgga
     5041 gtaaatggag aatcgttaca tgaaacattt ttttgcagga attggtgaga taaaacttgt
     5101 tggctactta ttgtatatat gtgtaggact agcccctctt tttcatatat atgtcatagg
     5161 ttcggaactg agttttgtga aaatagtgtt atctattttg ggagttatat ttgtaagtat
     5221 gttaactatt gctcgaattt ataggacttt tttctataaa gaatagtttg gacttgaact
     5281 gtatctaaaa acagaggttg gtcgattatc agcctctttc ttgtcgttga aaagagtgag
     5341 atatattgac ttttgaaaaa aagatgtcat aattgagttg ggataaacct aaaagtaata
     5401 tagtagattg aatctggaat agtccgttat gactgttaaa atatttctag aaattaattt
     5461 gactttccta atcgatttgt tcacatcttg tttcaatcta ctatactttc tcgttaaacc
     5521 gttcggcaat tcatggtgac atggacacat ggtagtaagc acactatgtg gcttggcaga
     5581 gctaaaaact gttcccttgc aataagccta ataagcacaa catagggagt tagagaagcc
     5641 gactctaatc aaccactttg ggcaatagtg agaactgccc tgtgcttttt tattttgaaa
     5701 aaatatggag tttgtcgttg aaattacttg attgtatttt ggactatcaa gaaagattca
     5761 atggaaaaac atgtcaagta tcaacgaatt ataagtattt agagattttc aaagtgaatt
     5821 tttgcttgac tgatttacat catttatttg gcttacacaa aatcacacga gattatgcta
     5881 gtcaaacaaa acctgctatt caaactggtg tttttatttt agaagatttt agaaatatcc
     5941 tattgtacaa tgatgtaatc gaaaggatat ctttatatga gtttataagt gatattttct
     6001 attctaaaat aacaagttgt tgcatcgtag ctaaggattt atctaaaaat actatgaaat
     6061 tggacgtcat attttttgag gataaaaata aaagatccgc agttttaggt ttacgaagag
     6121 ataaaagcgg agtatttaaa ccagttactc tacattttac aagcgctaag aaatatgcta
     6181 aagttcgtaa aacagatgtg aaagaaatga aatggctata aagattgtcc gcaggcttaa
     6241 gcttgcgttt tttgttgttt aacgaaatta aaaaataagc tctctcatcg gaaatgtttt
     6301 tataggaatg aaggaagatg atgctatgtt taaaaagaga atcgaaaagg gcaaaagtag
     6361 tgtctttatc tttttggagt agaagcttat gatatagaaa ttgtaataaa aatgtaatat
     6421 aaaacatttg acaaatattt acggagggag tatacttaat ataagtatag tctaaaaatg
     6481 actatcagaa aagaggtaaa tttagatgaa taagaaaaaa atgattttaa caagtctagc
     6541 cagcgtcgct atcttagggg ctggttttgt tacgtcttcg cctacttttg taagagcaga
     6601 agaagctccc gtagctagtc agtctaaagc tgagaaagac tatgatgcag cgaagaaaga
     6661 tgctaagaat gcgaaaaaag cagtagaaga tgctcaaaag gctttagatg acgcaaaagc
     6721 tgctcagaaa aaatatgacg aggatcagaa gaaaactgaa aagaaagctg ctgcggtaaa
     6781 gaaaattgat gaagaacatc aggcggctaa tcttaaatct cagcaagcat tagttgaatt
     6841 cttagctgct caaagagaag gtaatcctaa aaagaaaaaa gctgcgcaag ccaagttaga
     6901 agaagcagaa aaagcagaaa aggagaagaa aaaggaattt gataaagcac aggcagtggt
     6961 agttcctgaa gcaactgagt tagctgagac taagaaaaaa gcagacgaag ctaaagtaaa
     7021 agaaccagaa cttactaaaa aactagaaga agctaaagca aaatcagaag aagctgagaa
     7081 aaaagctact gaagccaaac aaaaagtgga tgcagaacat gctaaagaag tcgttcctca
     7141 agctaaaatc gctgagttgg aaaatgaagt tcagaaacta gaaaaagatc tcaaagagat
     7201 tgatgaatct gactcagaag attatgttaa agaaggtctc cgtgctcctc ttcaatctga
     7261 attggatgcc aaacaagcta aactatcaaa acttgaagag ttgagtgata agattgatga
     7321 gttagacgct gaaattgcaa aacttgaaaa aaatgtagaa gatttcaaaa actcaaacgg
     7381 tgagcaagct gaacaatacc gtgctgcagc tgaagaagac ttagctgcta aacaagctga
     7441 attagaaaaa actgaagctg accttaagaa agcagttaat gagccagaaa aaccagctcc
     7501 agctccagaa actccagccc cagaagcacc agctgaacaa ccaaaaccag tgccggctcc
     7561 tcaaccagct cctgcaccaa aaccagagaa gccagctgaa caaccaaaag cagaaaaaac
     7621 agatgatcaa caagctgaag aagactatgc tcgtagatca gaagaagaat ataaccgctt
     7681 gactcaacag caaccgccaa aagcagaaaa accagctccc gcaccaaaac cagagcaacc
     7741 agctcctgca ccaaaaatag gctggaaaca agaaaacggt atgtggtact tctacaatac
     7801 tgatggttca atggcaacag gatggctcca aaacaatggt tcatggtact acctcaacgc
     7861 taatggttca atggtgacag gatggctcca aaacaatggt tcatggtact acctcaacgc
     7921 taatggttca atggcgacag gatggctcca aaacaacggt tcatggtatt acctcaacgc
     7981 taatggtgct atggcgacag gatggctcca aaacaacgat tcatggtatt acctaaacgc
     8041 tagcggcgct atggcaacag gttgggctaa agtcaacggt tcatggtact acctcaacgc
     8101 taatggtgct atggctacag gttggctcca atacaacggt tcatggtact acctcaacgc
     8161 taacggtgct atggcaacag gttgggttaa agtcaacggt tcatggtact acctcaacgc
     8221 taacggttca atggcaacag gttgggtgaa agatggagat acctggtact atcttgaagc
     8281 atcaggtgct atgaaagcaa gccaatggtt caaagtatca gataaatggt actatgtcaa
     8341 tggtttaggt gcccttgcag tcaacacaac tgtagatggc tataaagtca atgccaatgg
     8401 tgaatgggtt taagccgatt aaattaaatc atgttaagaa catttgacat tttaattttg
     8461 aaacaaagat aaggttcgat tgaataaatt tatgttcgta ttctttaggt acctatctta
     8521 tgatttcagg aaatgtcatt aaaaaaacga ctccttttct ctaacttgaa aaatagatta
     8581 gagaaaatgg gttgttttat ttattatagt tatttgaatg aagataagaa gaaggtatac
     8641 tcacgtcatt cacatgatct gtatattgac tataagtttt aaaaaacaat ttttaagctc
     8701 ttccttgtct tctctaacca agcgtgttat aatgaatact gctcaagcga ccttcaatcg
     8761 tgaagcacac acgaccttca atcgtgaata aacgaataga tgggagactt accatgagtg
     8821 ataactctaa aacacgtgtt gtcgtgggga tgagtggtgg tgttgattcg tcggtgacgg
     8881 ctcttttgct caaggagcag ggctacgatg tgatcggtat cttcatgaag aactgggatg
     8941 acacagatga aaacggcgtc tgtacggcga ccgaagatta caaggatgtg gttgcggtgg
     9001 cagaccagat tggcattccc tactactctg tcaattttga aaaagagtac tgggaccgcg
     9061 tttttgagta tttcctagcg gaataccgtg cgggacgcac gccaaatccg gacgttatgt
     9121 gcaacaagga aatcaagttc aaggcctttt tggactatgc tatgaccttg ggggcagact
     9181 atgtagcgac tgggcattat gctcgagtgg tgcgtgatga ggatggtacc gttcacatgc
     9241 ttcgtggcgt ggacaatggc aaggatcaga cctatttcct cagccaactt tcgcaagaac
     9301 aacttcaaaa aacgatgttc ccactaggac atttggaaaa gcctgaagtt cgaaaactag
     9361 cagaagaagc aggtctttcg actgctaaga agaaagactc gacagggatt tgctttatcg
     9421 gagaaaagaa ctttaaaaac tttctcagca actacctgcc agctcagcct ggtcgtatga
     9481 tgactgtgga tggtcgcgat atgggcgagc atgcaggtct tatgtactat acaatcggtc
     9541 agcgtggcgg actcggtatc ggtgggcaac acggtggtga caatgcccct tggttcgttg
     9601 tcggaaaaga tctaagcaag aatattctct atgtaggaca aggattctac catgactcgc
     9661 tcatgtcaac tagcctagaa gccagtcaag tccactttac tcgtgaaatg ccagaagagt
     9721 ttacgctaga atgtacggct aaattccgtt accgtcagcc tgactctaag gtgaccgtcc
     9781 atgtcaaagg agacaaggca gaagttatct ttgcagaacc acaacgcgcg attacaccag
     9841 gacaggcagt tgtcttttac gatggcgaag agtgtctagg tggcggtttg attgacaatg
     9901 cttaccgcga tggacaagtt tgtcagtaca tttagattga caaattttct caatttgcta
     9961 caataataac agcaatagaa atgatggtca aagctcatgg atgttgcagg cttttttgtc
    10021 ctgcacttct ttgtagtttt gactgttttt gtgtcgttta agggaaagga caagaatgac
    10081 tcaacaagac tttcggacaa aagtagacaa tacggttttt ggtgttcggg ctacagcctt
    10141 gattgtccaa aatcacaagc ttctagttac caaagacaag ggcaagtatt acactattgg
    10201 aggtgcgatt caagtcaatg aaagcacgga atacgcggta gtccgtgaag tgaaggaaga
    10261 actgggtgtc aaagctcaag ctgggcagct agcttttgtg gttgaaaatc gttttgaaca
    10321 agacggcgtt tcctatcaca atatcgagtt tcattatctg gtggatttgc ttgaagatgc
    10381 cccgttgacc atgcaggaag atgagaaaag gcagccctgt gagtggattg acttggataa
    10441 gcttgaggat atccagctag ttccagcctt tttaaaaacg gccctaccag attgggaagg
    10501 ccaactaaga cacattcatc ttgaagaata ggagagaaac atgacttatc attttactga
    10561 agaatacgat attattgtaa ttggtgcggg acacgctggg gttgaggctt ccttagctgc
    10621 tagccgtatg ggctgtaagg tcctgcttgc gactattaac attgaaatgc tggctttcat
    10681 gccttgtaat ccctctatcg gtggttctgc taaggggatt gtcgtacgtg aagtcgatgc
    10741 cctcggtggc gagatggcca agaccattga caagacttac atccagatga agatgctcaa
    10801 cacagggaag gggccagctg tccgtgctct tcgtgcgcag gctgacaagg aactttactc
    10861 taaggagatg cgcaagacag ttgaaaatca agaaaatctg acccttcgtc aaaccatgat
    10921 taatgagatt ttggtggaag atggcaaggt tgtcggtgtg cgtacagcca cccatcaaga
    10981 atatgctgct aaggctgtta ttgtgacgac agggactgct ctccgtgggg aaattatcat
    11041 cggagacctc aagtactcat caggtcctaa ccacagcttg gcttctatta acctagctga
    11101 caatctcaag gaactgggcc tcgaaatcgg tcgtttcaag acaggaaccc ctccacgtgt
    11161 caaggcttct tctatcaatt acgatgtgac agaaatccaa ccaggagatg aagcgcctaa
    11221 tcacttctca tacacttcac gtgatgagga ttatgtcaag gaccaagtgc catgctggtt
    11281 gacctacacc aatggtacca gtcatgagat tatccaaaac aacctccacc gtgcgcctat
    11341 gtttacaggt gtggtcaagg gagtggggcc tcgttactgt ccgtcgattg aagacaagat
    11401 tgtgcgcttt gcggacaagg aacgccacca actcttcctt gagccagaag gacgtaatac
    11461 agaggaagtc tatgtgcaag gcctctcaac cagtctacct gaggatgtcc agcgtgactt
    11521 ggttcattcc attaaaggtt tggaaaatgc agagatgatg cgaacaggtt atgccattga
    11581 gtacgacatg gtcttgcctc atcagttgcg tgcgactttg gaaaccaaga aaatctcagg
    11641 tctcttcact gctggtcaga caaatggaac atcaggttat gaagaagctg ctggccaagg
    11701 gattatcgcg ggtatcaatg cggctttgaa aatccaaggc aagcctgaat tgattctaaa
    11761 acgaagtgac ggttatatcg gggtgatgat cgacgacttg gtgaccaagg gaaccattga
    11821 accttaccgt ctcttgacca gtcgtgctga atatcgtctc attcttcgcc atgacaatgc
    11881 tgatatgcgc ttgactgaga tgggacgcga gattggcctt gtggacgatg aacgctgggc
    11941 tcgttttgaa atcaagaaaa atcaatttga taatgagatg aagcgcctag acagtatcaa
    12001 actcaagcca gtcaaggaaa ccaatgctaa ggttgaggaa atgggcttca agccgttgac
    12061 agatgcggtg acagccaagg aattccttcg ccgtccagaa gtttcttacc aagatgtggt
    12121 ggccttcatc ggaccagctg cagaggaatt agatgacaag attatcgaat tgattgaaac
    12181 agaaatcaag tacgaaggtt acatctcaaa agccatggat caggttgcta agatgaagcg
    12241 catggaagaa aaacgtattc cagccaatat cgactgggat gacattgatt ctatcgcaac
    12301 cgaagcccgt cagaagttca aactcatcaa tccagaaacc atcggccaag ccagccgtat
    12361 ttcgggagta aacccagcag atatttctat tttgatggtg tatctggaag gtaaaaatcg
    12421 tagtatttct aaaactcttc aaaaatcaaa atgatatgtc gtaggcttct tacgaatgag
    12481 ttcaaagctt ggctttgatt catctccaat ctcccatagt tccccgaact atgggagcta
    12541 actcaagtta tgcattttcc tagccttctg gtctgatttt cattgagttt taatctaaaa
    12601 agaaagcata gagaaaaaca gctccgaaga cggggctgtt ttgttgattt gtactcaatg
    12661 aaaatcaaag agcaaactag gaagctagcc gtaggttgct caaagcactg ctttgaggtt
    12721 gtggatagaa ctgacgaagt cagtaacata tatacggcaa ggcgaagctg acgtggtttg
    12781 aagagtatta ttcttcatct ggtgtgagga tcatcgggat gatgatcggt tcacgttcgg
    12841 tattttcata gaggaagggg cgaatagcgt tgacaatggc accattgaca gattgcacgc
    12901 tagcatcctt atttttcagt gcgatacgaa tggcattgaa gaggatacgc tggctttggc
    12961 gaatcaagtc tccagactct ctcatgtaga caaagcctcg gctgaggata tctgggccag
    13021 acagaatcat ctgcgatttg aagtcaacag ttgcgactgc tagaacgaca ccgtcttcag
    13081 atagatcgcg acgatctttg aggacagctg cgccaatttc accgatacga tttccatcga
    13141 catagatatc ttgggcgttg aaatgacctg cgatacgagc tgagtcagca gtaagggcaa
    13201 gcacatcgcc attgctcatg ataaagatat tgtccttctc aacaccagta tccactgcta
    13261 gtccagcgtg gactttttgc atgcggtatt caccgtggac aggcatgaag tattttggct
    13321 taatcaagcg gagcatgagt ttttgctctt gctggccacc gtgtccagat gtatggatat
    13381 tgttcacttt accgtggata acttcgacac cagcttcaga aatgatgtta atcagcttgt
    13441 tgacgctagt agtgtttcca gggatgggac tagaagagaa gataacggta tcacctggtt
    13501 gtaattgtac ttgacggtgg gttccgttgg cgatacgaga gagggctgcc ataggctcac
    13561 cctgactacc tgtacagagg ataagaactt ctcctgcagg ataatctttg atttcatttg
    13621 gctcgataaa ggttccctta ggagctttga tgtagccaag atcgattccg ttgacaatgg
    13681 ccttttccat agaacgacca aagaccgcaa tcttgcgtcc agtcttaata gcagcttctg
    13741 ttgcctgctg gagacggaag atatttgagg caaaggatgc aaagatgata cgtccttcaa
    13801 taccttggat aatcttcata atggactgac caacgacttt ttcagagttg gtaaaggttg
    13861 gtacttccgc atttgtcgag tcagacagga gacagagcac gccttcttca ccaagggcag
    13921 ccatacgatg caagtccgca ggttctccaa ctggagtaaa gtcgaactta aagtcacccg
    13981 tacagacgat tttcccttga ggagtatgaa tgacaatccc caaaggctct ggaatagagt
    14041 gagtcgttct aaagaaagtt gccttgagat ttttaaaggt caactcggtg ttgtggttga
    14101 tttcgtaaag tttggcgttg cgcaagaggc cgtgttcttc gagtttccca cggatcaaag
    14161 ccaaggcaag cggtccagcg tagataggga catttgcttg cttgagtagg aacggaatcc
    14221 caccaatgtg gtcctcgtgt ccgtgtgtga ttaaaacagc cttgacgcgg tcgatattgt
    14281 ccacgatgta agagtagtca ggaatgacat agtcgatacc aagcaagtca tcttctggga
    14341 atttaatccc agcatcgacg atgataatct cgtcttggta ttcaattccg taagtgtttt
    14401 tcccgatttc tcctagacca ccgatggcaa aaacgccgac ttcttcaggt ttaagagtgt
    14461 aggccatatt agaactccgt aatttcgaag gcgccagttt ctttttcgta atcgagcaat
    14521 ttgtcagaca agagttcgat atactcgata ttgtactctg ggcgattttc ttcgacaagt
    14581 tggcgagcag tgatacggcc ctcaagttct gagctggcat cgatgtctag gtaaagcgtg
    14641 cgtgttgttt cacggcgtgg gctacgttct tttgtttctt gataaaaaac tttgtaaatc
    14701 atatagttcc tttctattta caggtcagct tattccaaac ttttagcaga tatttgcttg
    14761 atttcattcc attttgtgtc tagattgacc ttgattcgtt acaattattt caattatacc
    14821 acaaaatgaa aaattagtaa aagacggaaa cgagaaagtt aggggacgat ttctttggta
    14881 agcgtttgca aaaataaaga aaatgtatta tgttgataac ggaagtgaaa tttattgctt
    14941 gtcataattc catatagatt ttaagtcaag gaggttgccc tatgtataac ttatttgctt
    15001 ttgctatcgg agctcgcttg gtagctttta ttgtttttgt tgcttgcgtt tatgcgggga
    15061 atcttctatt tgccaaactg gaacgatgac aaaccaagtt cttgtggaaa attacctttg
    15121 tgactctcta ctcgcttttt ctcctcctcg tgacctatgt cgtttggttt gtattttact
    15181 ttggattaaa tgcttagatg ccctgcttat gctggggttt tttgtttggg gatgtagaga
    15241 tagtagattt acgacacgaa aattccaccc catctatcaa gatgaggtgg tctttgcatt
    15301 tttctaacat gacatgacaa tggaattttc aaagcttatt atttttatgg gagtcattgt
    15361 tctgctcaaa aatagagttg aagcataaaa ataaaacaat acaaacagat aaatcgaatg
    15421 gtaaagggat agtagtaatg ttcaaatcat tgagaatgct gagcaaactg taagagatga
    15481 agacaataaa atttatcctt ctaaaaatgt gtttcttatc tagcatgact taccaaactt
    15541 tttacgaagg tcttttccaa tggtataacc tgccataaat aaagcgatac caacacctac
    15601 accaaagcca cccttgattt cttgcaattc cttattagaa atttcacgat agttgttcaa
    15661 atctttcatc atagttaatc tcctcctaaa aaataaccag ctgccaaacc agaacctata
    15721 agtgctgttc cacctgcaat agccttccaa gatactgcta ctccaaagat aaccaaggga
    15781 gcgagaccgc catcaatatc tgataattca ttttcagata aggataaaaa attgattgtc
    15841 aagaatgtca aaatttgtca ttgaactttc ctcctttttt cttgttcact tttatattcg
    15901 gaaaagaaaa ggaaaagtga gcatttttta aattattttt agttaagtga gggcactctt
    15961 gtctgggggc aaagaaaatt cccatgtgcc agcttttgta gtataatagt aagcagacaa
    16021 aaagatagga atgtgttatg aaagtattag cttttgatac gtccagcaag gctctttctc
    16081 tggctatttt agaggacaag caggttcttg ctgagacgac gattaatatc aagaaaaatc
    16141 acagtattac cctcatgcca gccattgatt ttttgatgga gagtttggac tggacgccca
    16201 aggatttgga ccgaatcgtg gtggcggaag ggccaggtag ctacacaggc ttgcgaatcg
    16261 cggtagccac tgccaagacc ttgactcata ctctgaacat tgagttagtt ggtatgtcta
    16321 gcctcttggc tctggtgcca tatcaacaag aaggcttgtt cgttcctttg atggatgcgc
    16381 gtcgcaacaa tgtttatgca ggattttatg aaaatgctaa acctgtcatg ccagaagcgc
    16441 acctatcttt tgaagaggtg ctagaaaaag tcaagggtgc tagtcaggta acctttgtcg
    16501 gagaagttgc cccctttgtg gagcagattc aagaacactt gccaaggact aattataaag
    16561 aaaccttgcc taatgcagcc aatcttgctc ttttagcttg ggacaaggaa gcagaatcct
    16621 tgcatgattt tgtgccaaat tacctcaaac gagtcgaggc tgaggaaaac tggctcaaga
    16681 accataccga gtctggtgag tcctacatta aacgcctatg atagaaatca agcgaattca
    16741 acaacagcct gacctagctc aagccatcta cgctgttatg gcagctgttt acctagtcag
    16801 tccttggact ctggagcaaa tccaagcaga tctgtcccaa gaccagactt ggtatgcatt
    16861 ggcttatgat ggggcagaag tgattggatt tctagctgtg caggagaatc tttttgaagc
    16921 agaagtcctg caaatcgctg tcaaaggagc ttatcagggt cagggaattg cgtcagcctt
    16981 gtttgctcaa ttgccgacag ataaggagat tttcctcgaa gtcagaaagt caaatcaacg
    17041 agcgcaagca ttttacaaga aagaaaaaat ggcggtcatc gctgagcgaa aggcctacta
    17101 ccatgaccca gtcgaggacg ccattatcat gaagagagaa atagatgaag gatagatata
    17161 ttttagcatt tgagacatcc tgtgatgaga ccagtgtcgc cgtcttgaaa aacgacgatg
    17221 agctcttgtc caatgtcatt gctagtcaaa ttgagagtca caaacgtttt ggtggcgtag
    17281 tgcccgaagt agccagtcgt caccatgtcg aggtcattac agcctgtatc gaggaggcat
    17341 tggcagaagc agggattacc gaagaggacg tgacagctgt tgcggttacc tacggaccag
    17401 gcttggtcgg agccttgcta gttggtttgt cagctgccaa ggcctttgct tgggctcacg
    17461 gacttccact gattcctgtt aatcacatgg ctgggcacct catggcagct cagagtgtgg
    17521 agcctttgga gtttcccttg ctagcccttt tagtcagtgg tgggcacaca gagttggtct
    17581 atgtttctga ggctggcgat tacaagattg ttggggaaac gcgagatgac gcggttggtg
    17641 agtcctatga taaggtcggt cgtgtcatgg gcttgaccta tcctgcaggt cgtgagattg
    17701 acgagctggc tcatcagggg caggatattt atgatttccc ccgtgccatg attaaggaag
    17761 ataatctgga gttctccttc tcaggtttga aatctgcctt tatcaatctt catcacaatg
    17821 ccgagcaaaa gggagaaagt ctgtctacag aggatttgtg tgcttccttc caagcagctg
    17881 tactggatat tctcatggca aaaaccaaga aggctttgga gaaatatcct gttaaaaccc
    17941 tggttgtggc aggtggtgtg gcagccaata aaggtctcag agaacgccta gcagccgagg
    18001 ttacagatgt caaggtcatc attccacctc tgcgcctctg cggagacaat gcaggtatga
    18061 ttgcttatgc cagtgtcagc gagtggaaca aagaaaactt cgcaggctgg gacctcaatg
    18121 ccaaaccaag tcttgccttt gataccatgg aataaagagt ggctctttgt caagtgtagt
    18181 gggtaggcga aaagctacaa tctggagagt acgaaattcg tactctcttt tttgatgttc
    18241 aaagtcatca aaatccgttt tttaaaattg ttaaagttcc ggaaaccaaa ggttttgcgc
    18301 ttgataacct taatcaaatt attggtcgct tcaagtttag cgttggaata aggcagctta
    18361 agtgcgttga taatcttatc tctatccctt aaaaaagtcc aaaagaccgt ttgaaaaagc
    18421 ggatgaaccg ttggcagttc ttgctcaatc aatccaaaga aatggtcggc attcttctct
    18481 tgaaaatgaa agagcaggag ttgatagagt tcgtagtgaa cctgtaatcc ctcagagtaa
    18541 gaaagcagct tatctcgtac ttcccgatgg gttaagtgca ttgaaacgtg ggctgataga
    18601 atcgtttctg acagagcttg aagctatcct ggtgtagtaa atcagtccag tggactgatt
    18661 tagcccgagc ctagaaacga aaaagcgagg gttccaaaag cgcttgagcg cccgatactc
    18721 taaggatttt cggtcaaatt ggttcatgat ttggattcgt actcggttca tagctcggct
    18781 cagatgttgg acaatgtgga agcggtcaag aacaatcttc gcctttggaa agagttgctt
    18841 agcgagccga taataagggc tgtacatgtc catggtgatg acctcgacca gctcccgaac
    18901 ctctctctga tagcgttgga aatggtttcg aatcaccgca taagttcgcc cgtctaaaat
    18961 tgcgaggatg gatttggact caaaatcttg ggcaatgaag ctcattttgc tctttttgaa
    19021 gctatactca tcccaactca taacttttgg caacttggtc caatcggttt caaacttaaa
    19081 ttccctcagt tttcggatga cggtggaagt tgagaccgcc aatctgtggg caatctcagt
    19141 catagactgc ttttcaagga ggagctgagc gattttttgt ctcaccatgt tggaaatctg
    19201 gcaatttttc ttgacaatgg atgtttgaga aacgaccacc ttaaggcaat tcttgcactg
    19261 aaagcggcgc tttttgagat gtagtaccgt gggtaaaccc tgacagtcga gaagcggaat
    19321 tttagaggct ttctggaagg cgtatttgat catcttccct tgacaatgag gacaaggagg
    19381 agcgggggaa tccaactttg cctgaacgac tagatgggtt tggtatttca gaacagacaa
    19441 gattttgata tttttgtctt tcaatccgag caaatctgtg gtattcttaa gttgttccat
    19501 atgaaccctt tctaatgagt tggttagacg cttttcatta taggtcatat ggggcttttt
    19561 ttctacaaga aacgacccta taattcctgg ggtgggatta cccactacag aaattataga
    19621 gccaaaacat tccaaagtct tgtctgactc tggaatgttt tctgttcaca gttgttttgt
    19681 aactgcctaa taagtaaccg cgctataacg aggtatctac cgagattgtc aattctccag
    19741 tatactgtta taaaaaacaa aactggagga tttttttatg tccccccatc gtcccatcga
    19801 ctgttccgta gctgcccaat aacggagcat agtcggctga aacaagaata ggacaaaagt
    19861 gcctcaagaa aagcatcgta atttgacaat attattctga gatgtttttg atgtaagccc
    19921 atattttctc aatgggatta tactcgggtg aataggtagg aagtggtaac agtctatgcc
    19981 ctgctcctta cacatgttca ttctgtgaaa ccttgcattg tccataatga taagggatgg
    20041 tgtatctaaa gtaggtagta agaatttttg gaagcaagct tcgaaaaagt cactcgtcat
    20101 cgtatcttta taggtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    20161 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    20221 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    20281 attcgagata aaaataggtc tcaacccctg tctcataaat ataaatagga gtcaggtagc
    20341 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    20401 tacggctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    20461 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    20521 tctttaattt atctctatca actttttcaa ttaatagtgg aagttttctt gaagttagtg
    20581 tattgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgt atttgtaata
    20641 gtattatagc aattctttgg agaaggtcga gacaagagaa aataggtatc tttgagatat
    20701 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    20761 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    20821 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    20881 tataataatg gacattgtgg aggcatagat ccgcatataa ttggactttg taaaaagcag
    20941 atcagaagga tgtctctata tcctattttg ataatcgtat ctagagattc aaaggttatt
    21001 gatgattata aaaatttaga tattgcctat gttgattgta ctcaatgttc aaataatttt
    21061 gaaactgcct tacacgttaa aaatatttta aaattattaa aaatcc
//
LOCUS       DBERCA010000028        20637 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.028, whole genome shotgun sequence.
ACCESSION   DBERCA010000028 DBERCA010000000
VERSION     DBERCA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.028"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1099)
                     /gene="phtB"
                     /locus_tag="ACL719_001212"
     CDS             complement(<1..1099)
                     /gene="phtB"
                     /locus_tag="ACL719_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HGS0699176.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVLKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPS"
     gene            complement(1257..3707)
                     /gene="phtA"
                     /locus_tag="ACL719_001213"
     CDS             complement(1257..3707)
                     /gene="phtA"
                     /locus_tag="ACL719_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGS0699177.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQTSQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3960..5693)
                     /gene="ptsP"
                     /locus_tag="ACL719_001214"
     CDS             complement(3960..5693)
                     /gene="ptsP"
                     /locus_tag="ACL719_001214"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGS0699178.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(5699..5962)
                     /locus_tag="ACL719_001215"
     CDS             complement(5699..5962)
                     /locus_tag="ACL719_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGS0699179.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            6313..6531
                     /gene="nrdH"
                     /locus_tag="ACL719_001216"
     CDS             6313..6531
                     /gene="nrdH"
                     /locus_tag="ACL719_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGS0699180.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            6612..8771
                     /gene="nrdE"
                     /locus_tag="ACL719_001217"
     CDS             6612..8771
                     /gene="nrdE"
                     /locus_tag="ACL719_001217"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGS0699181.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            8959..9921
                     /gene="nrdF"
                     /locus_tag="ACL719_001218"
     CDS             8959..9921
                     /gene="nrdF"
                     /locus_tag="ACL719_001218"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGS0699182.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            10103..10864
                     /locus_tag="ACL719_001219"
     CDS             10103..10864
                     /locus_tag="ACL719_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0699183.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(11149..11400)
                     /locus_tag="ACL719_001220"
     CDS             complement(11149..11400)
                     /locus_tag="ACL719_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGS0699184.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQMPCLTSEELKSLGKWYVSTGK
                     EWICHSDADLEEFKNLFLNFINPEEWDTISFDSDFMPFQ"
     gene            complement(11443..12849)
                     /gene="lacG"
                     /locus_tag="ACL719_001221"
     CDS             complement(11443..12849)
                     /gene="lacG"
                     /locus_tag="ACL719_001221"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS0699185.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(13064..14758)
                     /locus_tag="ACL719_001222"
     CDS             complement(13064..14758)
                     /locus_tag="ACL719_001222"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS0699186.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(14760..15077)
                     /locus_tag="ACL719_001223"
     CDS             complement(14760..15077)
                     /locus_tag="ACL719_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0699187.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            complement(15113..15949)
                     /locus_tag="ACL719_001224"
     CDS             complement(15113..15949)
                     /locus_tag="ACL719_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS0699188.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(16183..17163)
                     /gene="lacD"
                     /locus_tag="ACL719_001225"
     CDS             complement(16183..17163)
                     /gene="lacD"
                     /locus_tag="ACL719_001225"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGS0699189.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            complement(17165..18094)
                     /locus_tag="ACL719_001226"
     CDS             complement(17165..18094)
                     /locus_tag="ACL719_001226"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGS0699190.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQDPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            complement(18105..18620)
                     /gene="lacB"
                     /locus_tag="ACL719_001227"
     CDS             complement(18105..18620)
                     /gene="lacB"
                     /locus_tag="ACL719_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGS0699191.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHHESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            complement(18651..19076)
                     /gene="lacA"
                     /locus_tag="ACL719_001228"
     CDS             complement(18651..19076)
                     /gene="lacA"
                     /locus_tag="ACL719_001228"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGS0699192.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(19233..19730)
                     /locus_tag="ACL719_001229"
                     /pseudo
     CDS             complement(19233..19730)
                     /locus_tag="ACL719_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(19746..20549)
                     /locus_tag="ACL719_001230"
                     /pseudo
     CDS             complement(19746..20549)
                     /locus_tag="ACL719_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(20557..>20637)
                     /locus_tag="ACL719_001231"
     CDS             complement(20557..>20637)
                     /locus_tag="ACL719_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS0699193.1"
                     /translation="PILFCIPAMSVPVFDNIILPAKQNMV"
BASE COUNT         6093 a         4246 c         3699 g         6599 t
ORIGIN      
        1 gacttggact aggttccgga gtcgattgtg gacttggttg ttctggtctt gaatctggta
       61 cccaatggtt tgaacgataa cgaaggggaa taatacgagc aattcgtttt tccaattcag
      121 acatttgttc ataagggata aagtggtaat ggttaccatg agggacagct acacctctgg
      181 cggttcgact tgtgatttgc gctgggtcga aaataaggcc atcagattcc acatggcgtt
      241 ctgataaggg tttagcatac aattcacgta aaaggcttga aatgttttcc ccttgatttt
      301 gatgataagt tggagtgaca gtcagattgt ggttctctga caatcttggt tgagctggat
      361 ttgcattata actagaactt gaagaaggac gagatccctg cttcccattc caataggctt
      421 ctgcagcagc taactcgcta gctgataact cattcttagg aatgtaatgg taatggtcgc
      481 cgtgaggaac gatataagca tcacccgtgt cctcaatgat atcagatgca ttgaagatat
      541 acccatcatc cgttgtataa cgtccttggg ctctggctgc agcaacagca ttatctgctc
      601 ttgagttatg attatgactg cgttcctgct tctgacgttt aatctcttct tttgtccgaa
      661 tattatccgc atgagctgca tccttaaggt aaacatagta tttaccgttt accttaagga
      721 cataaccacc cttgatttca ttgacaatgt ctgaatcctt caactgataa ttcggatctt
      781 tcatgaggag ctcttcactg atgatggcat cataaggaac cttgccatta tagtaatgat
      841 aatggtctcc atgagaggtc acataacctt gatccgtaat cttgataaca atttgttcgg
      901 cgttgatccc ctccctctta ctgacttcat ctggtgtcaa gttttctgcc ttttgaccag
      961 cctgatcacc atctatataa gcaactcgat tagactcttt cttatcctga ccagcttggt
     1021 aacgtccaag ctcataggaa caaacactta gggcaaggac tgccactgaa cctgctagat
     1081 attttttatt gattttcatt ctttcctctt ttcttaagta tatttgattc actataaagt
     1141 aataaaccgg ttaattaact agttaatagt ttactctttt taatattgct tgtcaagaat
     1201 tttacatata tacataataa aaatgaaagc ctctttatcg agactttcat ttttcattag
     1261 tttatttttt ccttacttac agatgaagga ttacttcctt ttaacaacgc aagtaatttt
     1321 tctgcttctg ccatgatact attgttatcc ataatttgaa gagtcaaatt atttcgtaaa
     1381 ccagctagag tttctgttgc attggctttc agactagaat ccgttacttt cgcaagcaaa
     1441 acttctgctt ctttgagttg ggcttctact ttttcagtct ctacttgagg gacttctggc
     1501 tcagcaggtg tttcctctac tggctcttca tccgctttga agttcttatt tggatcttca
     1561 ctgtggtctt tcttgcctaa cacatgctca ctggcattgc cccatccatc attagaatgt
     1621 ggacgttcgt cagggtgttc tacgtagtac ttaatcgtcg caaacaaatc ttccaaggta
     1681 tagccatttg gagctttgta tgtgtgatca tcaaaccaag caaatttaat gttatggtaa
     1741 tgatccttat gaggaataat caaattaccg tttttaacct caactgtatg ctcaaccata
     1801 tatggaagtc gaacgagtgg aattcgtttt tcccctttca cacgattgta aatagctgct
     1861 gcactatctc cagttggatt tgctttaaca tctgcgtctg gagatggagg taggatacct
     1921 ttttctttag tataggcttg agctgcaact ttttccttat cagaaaggct gtcttttcca
     1981 atccagtgac tatggcccat atgaggcgtt acatatgcat ctccttcatc actgattata
     2041 tcatgttcat caaaaatata accatctgac gttgtatact tatcagctaa ttgagcaata
     2101 cgaacttcgt cttcagtata ctcaatttga gaatttggct tgccaagtcg ctctggatgg
     2161 gtaattggtg ctaggaatgc caataaatca tctaccaatt tttctttatt agtcgattca
     2221 tcattcaagc gttctaataa tttgtctaag gcttggaaat cagaattacg acccttattt
     2281 tcaaacaagg ctttatgagc ctcagttaac agattatatg ctttatcata aaattcttgg
     2341 tcacgaggag caacattttc ttttttagca gttaaagtgt gtgaaacact ctcttgtttt
     2401 gataacttgc tttcaagatt tttaacagtt tcagatggta aatctttcgc aaagacataa
     2461 cgagagatgc ccttttcttc gaatacatat ccttccccaa cttttcgtac cagctgacta
     2521 accaaagaag aatttgagtc tattttaaga tttggtgcag gttgcgggcc tggactaggt
     2581 tccggagtcg gttgtggact tggttgttct ggccttgaat ctggtaccca atggtttgaa
     2641 cgataacgaa ggggaataat acgagcgatt cgttcttcca attcagacat ttgagagtaa
     2701 gggatgaagt ggtaatgatc tccgtgtggc actgcaacac ctctagctgt tcgacttgtg
     2761 atttgcgctg ggtcgaaaac aaggccatca gattctacat gtcgttgact caaaggcagt
     2821 ttgtagagct gtttcaagag actatcaatg tcattacttt gacttgtttg actgttagtg
     2881 ttgctgttgt tgcttgtgtt agtatttgta gttcctggat tgcttacaga aggtacccag
     2941 tttgttcttg aagtgttatc gctattttgt cggcgatagg ttcttgaatt tgacagattt
     3001 ccccgaccag ataggaaggc ttctgcagca gccaactcgc tagctgataa ctcattctta
     3061 ggaatgtaat ggtaatgatc tccatgagga acgatataag catcaccagt atcctctatg
     3121 atatcagaag cattaaagat ataaccatca tctgtagtat agcgtccttg cgaacgtgcc
     3181 aaggcaacag caccatcgtt tcttggagtc ccaccttcac gatgttgact atgttcttgt
     3241 ttttgtcgat tgatttcctc ttttgtacgg acgttatccg cgtgggcagc atccttaagg
     3301 taaacatagt attttccatc taccttgata acatatccac ccttgacctc attaacaata
     3361 tcctcatctt ttagcttata gttcggatct ttcatgagta attcttcact gaaaatcgca
     3421 tcataaggaa ccttaccatt gtaataatga tagtggtcgc catgtgaagt gacatagcct
     3481 tggtctgtta tcttgatgac gatttgctca gcattgattc cttcacgctt gctaacctca
     3541 tcaggagtca aattctccgt tttttgcgtc gcttgttttc catctatata ggaaacacga
     3601 ttattttcct taaccgttct agcttgatac agtcccaact cgtaagaaca aacacttaaa
     3661 atcaaagctg ccgcagaacc aacaaggtat ttcttattaa ttttcatcga aactctcttt
     3721 ctatttgata tttctagttg caattcattt ctaaattgca gaaactcctc tctattactt
     3781 aactggttaa tagtttactc ctaaatctcc aacttgtcaa gaattttatg aaccatttaa
     3841 gtattttttg ttctgactac tactttttca agaaaatctt tctataagaa agattttctt
     3901 gaaaattctt tttaaaatat caaaaaaatc cctgtaactg agttgcaggg acttttcgat
     3961 taatcaaaat taacgtattc tttttgaagt tcaagaactt cttccattgt tgagcattct
     4021 gtaagggcac ggtttgcgta ctcttccatc ttagctgtgt cgagtttctt catcaagctg
     4081 cgtgtacgaa gtacagatgt tgctgacata gagaactcat ccaagcccat tccgacaaga
     4141 agtggaacag cttgttggtc accagccatc tcaccacaca taccagccca tttaccttca
     4201 gcgtgagctg ctttgatcac attgttaatc aagcgtagga ttgatgggtt gtatggttgg
     4261 taaaggtatg aaacttgttc gttcatacgg tctgctgcca ttgtatattg gatcaagtcg
     4321 tttgtaccaa ttgagaagaa gtcaacttct ttagcaaatt ggtctgcaag catagccgct
     4381 gcaggaatct cgatcatgat accaacttgg atgttatccg caactgcaac accttcagca
     4441 agaaggtttg ctttttcttc atcaaagact gctttcgctg cacggaattc tttcaagagc
     4501 gcaaccattg ggaacatgat acgcaattga ccgtgaacag acgcacgaag aagagcacgg
     4561 atttgtgtgc ggaacatagc atctccagtc tcagagatag agatacgaag agcacggaat
     4621 ccaaggaatg ggttcatttc gtgaggcata tcgaagtaag gaagttcctt atctccaccg
     4681 atatccattg tacgaacgac aacaggttta ccgttcattc cttcaagaac agccttgtat
     4741 gcttcatact gctcatcttc agttgggaag tcttgagaat ccatgtacaa gaactctgta
     4801 cggtaaagtc caacagcttc tgcaccgttg ttgttaacac cttcaacgtc ttttggagta
     4861 ccgatattag cagccaactc gaagtgttta ccgtcagcag tcactgtttg agcatctttc
     4921 aagagcgccc attcagcttt ttgtttcgca taagcttcac cagctgcttt aaattctgcc
     4981 gcttgttcat cagttgggtt gataataacc tcaccagtga tcccgttaac agcaaggatg
     5041 tcaccgtctt taacgatttc agtgatgttg tttgtaccta atacagctgc aatttcaagt
     5101 gtacgtgcca tgatagctga gtggcttgta cgtccaccga tgttggttac aaaagctttt
     5161 acaaagtttt tgtccaattg agctgtatca gatggtgtca agtcatgtgc aatcacaatc
     5221 acttcttcat tgatagaagc tgggtttggc aattttttac caagaaggtt tgccaataca
     5281 cgttttgtca catcgcggat atccgctgcg cgttcttgca tgtatgggtt gtcttccatg
     5341 ccttcaaaga tagtgataaa catatctgta acttctttca gacctgcttc tgcattcact
     5401 ttcttcgcac ggatagtttc cttgatttgg ctgatcattt ctgggtcagc aagaaccatt
     5461 aagtgagcat caaaaacttg agctgcttct tcaccgagcg tacctactgc tttctcgcga
     5521 ataacagaaa gctcgtcttg tgatgcctgt agagcggcat caaggcgagc ttcttctgcg
     5581 tttgtatctt cgactgtaat agtctcaaat gacaaatccg gctgaacgag tagatatgct
     5641 tttgcaactg caacaccgtc agatgctgcg attcctttaa gcatttctgt catttccctt
     5701 atgccaatcc ttctttttcc attgtttctg agattgcagc gatagcgtca tctgcatctg
     5761 caccttcagc tgagatagtt acgtcagcac cttggccaac accaagactc ataacaccca
     5821 taattgattt aaggttaact gatttacctt tgtactcaag agtgatatct gaagcaaatt
     5881 tgctagcagt ttgtaccaac aatgttgctg gacgtgcgtg aatacctgtt tctgccacta
     5941 cgtggaaatc tttagaagcc atagtttgac tctccttttg ttctttcttt ttttgagtta
     6001 tatgtgataa cccttacaat ttagtattat accatcttct aggatttttt tcaagcattt
     6061 tctggatttt ccttcaattt cctttcagat aacttgctga aaatcttcta tcctagataa
     6121 caaaacacag gatatagttt ctttaaaaac aatttatagg ataatcattg ctatttcaca
     6181 atacaaacac tatatattgt gtttatatat aaaaacaaca aaagaacacc actatattta
     6241 gttcaaatca ttgacaaaat tttattttct gatatactaa gatagtatta aattttgaag
     6301 aggagttaca caatggtaac cgtttattct aaaaacaatt gtgtccaatg taaaatgacc
     6361 aagcgtttct tggacagtaa taatgtctct tatcgtgaaa tcaatcttga tgagcaacct
     6421 gagtacgtcg atcaagttaa agagctcggt tttagcgcag ctcctgttat ccaaacacca
     6481 actgaagtct tttcaggttt ccaaccagga aaactgaaac aattagcata atcttagtac
     6541 atcatccaga agaaactgct tttagggcta acttagaagc ctttcttttg taattagata
     6601 agggaaattt tatgggatta aaacatcttg aagacgtgac ttacttccgt cttaataacg
     6661 aaattaaccg tcctgttaat ggacaaatca tgcttcataa agataaggaa gccttggatg
     6721 ctttctttaa agaaaatgta gttccaaaca ctatggtttt tgattcaatc aaagacaaaa
     6781 tcaattacct cattgaacac aactacatcg aaacagcctt tatcaagaaa taccgtccag
     6841 agttcttgga agaattggct caatttatca aagaccaaaa cttccaattc aagtcattca
     6901 tggcagctta taaattttac aatcaatatg ccttaaagac taatgacggt gaatactatc
     6961 ttgaaaatat ggaagaccgt gtcttcttca acgcccttta tttcgctgat gggaatgaag
     7021 ctgttgcaat cgatattgcc aatgaaatca tccaccaacg ctaccaacct gctactcctt
     7081 ccttcttgaa tgctggacgt gctcgtcgtg gggagttggt atcttgtttc ctaatccaag
     7141 tgacggatga tatgaactct atcggacgtt ctatcaactc agctcttcaa ctttcacgta
     7201 ttggtggtgg tgtgggaatt accctcagca accttcgtga agctggtgca cctatcaaag
     7261 gctatgaagg agctgcttca ggtgtcgttc ctgttatgaa gctttttgaa gacagcttct
     7321 cttactccaa ccaattgggg caacgtcaag gtgctggtgt tgtctacctc aacgtctttc
     7381 acccagatat catcgctttc ctttcaacta agaaagaaaa cgctgatgaa aaagtacgtg
     7441 tcaagactct atcacttggt gttgtagtac ctgataaatt ctacgaattg gctcgtaaaa
     7501 atgaagaaat gtacctcttc agcccttact ctgtagaaaa agaatacggt gtgccattca
     7561 attacatcga cattactgaa aaatacgatg aattggtcgc aaatccaaat attcgcaaga
     7621 caaaaatcaa ggcgcgggat ttggaaactg aaatctctaa attgcaacaa gagtctggct
     7681 atccttatgt agtcaacatc gatacggcta accgtgcaaa tcctgttgat ggaaagatta
     7741 tcatgagtaa cttgtgttct gagattcttc aagttcaaga accaagcttg atcaacgatg
     7801 ctcaagaatt ccttcaaatg ggaacagatg tttcatgtaa tctgggttca accaacgtgg
     7861 ttaacatgat gacttcacct gattttggtc gttctatccg tgctatggtt cgtgccctta
     7921 ctttcgttac agatagttca catatcgtag ctgtaccaac gattgaccat ggaaatagcc
     7981 aagcccacac ctttggtctc ggtgccatgg gactgcacag ctaccttgcc caacaactca
     8041 ttgaatatgg ttcacctgag tctgttgaat ttacaagcat ctactttatg cttatgaact
     8101 actggacctt ggtagagtca aacaatattg cgcgtgaacg tggtattacc ttccacaact
     8161 ttgaaaaatc agactatgct aacggaagtt actttgacaa gtatgtaact ggtgaatttg
     8221 ttccaacatc agaccgtgtt aaagaactct tcaaaaatgt ttttatccct ggtgttgctg
     8281 attgggctga acttcgcgac aaggttcaag aagatggtct ttaccaccaa aatcgccttg
     8341 ctgtagcgcc aaatggttct atcagctata tcaacgacgt ttctgcttct atccacccga
     8401 ttacgcaacg tatcgaagaa cgccaagaga agaaaattgg taaaatctac taccctgctg
     8461 ctggcttgtc tacagaaacc attccttact acacttctgc ctacgatatg gatatgcgta
     8521 aagtcatcga tgtttacgct gctgcgactg aacacgtgga tcaaggactt tcactcaccc
     8581 tcttcatgcg cagtgacatt ccaaaaggcc tttacgaatg gaagagggaa aataaacaaa
     8641 cgacacgtga tttgtccatc cttcgtaact atgcctttaa caagggaatc aagtctatct
     8701 actacgtccg tacctttaca gatgacggtg gagaagtcgg cgccaaccaa tgtgaaagct
     8761 gtgtgattta gtttctagca tagaaacatt atttttccct aagcagcact gataaaatca
     8821 aatctactat gtcataacat aatttatgta acattttaaa agtcgggctt ccgacttttt
     8881 gtgcgatact cctatagaat tatggtaaag tagaaataat tgtgagttcg caatactaaa
     8941 cacagaaaga gattttaaat ggaaacttac tacaaagcca ttaactggaa tgccatcgaa
     9001 gatgtcatcg acaaatcaac ttgggaaaaa ctgacggagc aattttggct cgatacacgt
     9061 attcccttat caaacgactt ggatgactgg agaaagctat ctaacaaaga gaaagacttg
     9121 gtcggaaaag tttttggtgg tttaaccctt cttgacacta tgcaatctga aactggggtt
     9181 caagcccttc gcgcagacat ccgtacacca catgaggaag ctgttttcaa taacatccaa
     9241 tttatggaat ctgtccacgc taaatcttac tcatcaatct tttctacctt gaatactaag
     9301 gctgagattg aagaaatttt cgaatggacc aataccaatc cttacctaca aaagaaggct
     9361 gagattgtca acgaaatcta cctaaacggc agcccacttg aaaagaaagt tgccagcgtc
     9421 ttcctcgaaa ccttcctctt ctactctggt ttcttcactc ccctctacta tctcggtaac
     9481 aacaaactag ccaacgttgc agaaatcatt aaattgatta ttcgtgacga gtctgttcac
     9541 ggaacctaca ttggttacaa attccaactt ggtttcaatg aattgcctga agaagagcaa
     9601 gaaaaactca aagaatggat gtacgaccta ctctatactc tttatgaaaa tgaagaaggc
     9661 tacacagaga gtctttatga cggtgttggt tggactgaag aggttaaaac cttccttcgc
     9721 tacaatgcca ataaagctct catgaacatg ggacaagatc cacttttccc agattcagct
     9781 gaagatgtca acccaatcgt tatgaacggt atttcaacag gaacttccaa ccatgacttc
     9841 ttctctcaag tcggaaatgg ttaccttctt ggcgaagttg aagccatgca agacgatgac
     9901 tataactacg gtttagacta attctccatc actctcaatc atcaaaaagt aaactagaaa
     9961 catcaaagtt ataacccaac aaaaatatcg aaagttttga aaacaaactt cgatattttt
    10021 gtttttgttt cattttgttg tttttgtatt tgaatgatga gtttgtttat ggtaagatag
    10081 tagtaggaaa cgaggtgata atatgctcaa acaagaaaaa ttaaccaaaa ttttagagat
    10141 agtaaatagt aaaggaacca taacagttaa acagattatg gacgaaatag ccgtttcaga
    10201 tatgactgca aggcgctatt tacaggaatt agctgataaa gatttgctga ttcgtgtgca
    10261 tggtggagct gaaaaacttc gaaccaactc ccttttgact aatgagcgat caaatattga
    10321 aaaacaagcc ctccaaacgg cagaaaaaca agaaatagcc cattttgcag gcagtctagt
    10381 agaagaaaga gaaactattt tcattggacc aggaacaaca ttagagtttt ttgcgcgtga
    10441 gttgcctatt gacaatatcc gcgtcgtaac caacagtcta cctgtttttc tgattttaag
    10501 cgaacgaaaa ttaacagatt tgattttaat aggtggaaat tatcgcgata ttacaggtgc
    10561 ttttgttggt acattgaccc tacaaaatct ctctaatctc caattttcta aagctttcgt
    10621 tagctgtaat ggtattcaaa acggagctct agctactttt agcgaggaag agggagaggc
    10681 tcaacgcatc gctttaaata attctaataa aaaatattta ctcgcagatc atagtaagtt
    10741 caataagttt gatttttata ctttttataa tatatcaaat cttgatacta ttgtttcaga
    10801 ttctaaacta agtgattcaa tcctttttaa gctatctaaa cacattaaag tcatcaagcc
    10861 ttaataaact gaaagagcct gggtaacaac tcaatttcag aagaaaaaaa gtaaatcttc
    10921 ccacaataaa aagcatagta tcaggtgttg aaacacctga tactatgctt ttttctatat
    10981 caaagacttt ttgcccaatt cctcaaagct atacattgag aaatctgcga ctcgcttcct
    11041 agtttaaagt tgctctttga ttttcattga gtataagcaa cgtgattaga taagagtttt
    11101 acagagtact aaatataaga tttaactttt ttgagaaatt ggttacgatt attgaaacgg
    11161 cataaaatct gaatcaaagg agatagtatc ccattcttca ggattgataa aatttaaaaa
    11221 tagattttta aattcttcca gatcagcatc tgaatgacaa atccattctt taccagtcga
    11281 gacataccat tttccaagac ttttcaattc ttcactcgtc aaacacggca tttgagagca
    11341 tttttcgaaa tttataatat aaactttttc ataaatagat tggataaaat ctttgaacat
    11401 ctttttgact tactaaaatt ctatatctaa tgactaattc tactagtcta taatctgagt
    11461 ttccgctact ttcttgtacc agtgagctga tttcttagga taacgttctt gagtttcaaa
    11521 atctacgtag aagagaccat aacgtttctc ataaccgttt gaccatgaga agacatccat
    11581 taatgaccaa atgaagtaac cttttacatt agctccatct gcaatcgcat cagacaagat
    11641 ctccaagtgt tgtttcacgt aatcaatacg accatcatcg taaacagtgt tatcaacgaa
    11701 ctcatcttta tagccgagac cattttcagt gatgtaaatc ttcttgtagt taggataatc
    11761 tttcttcaca cgcatgattt ggtcatacaa accttgaggg tagataatcc aatcccaatc
    11821 cgtgcgtggt acatagtcag gagctacacg acgaccaaca cctttgattt gatacttaga
    11881 gcttcctttt tcacctttac cattatggat aatttcagtt tctccatcaa aggcttccat
    11941 ccagtcactc atatagtagt tgattcctag gaaatcattc aagtcttttg cagcttctaa
    12001 tgctgtaaaa tcttcttcac gaagatccaa actaccacca ttgactgata agatatggtt
    12061 gacaccttcc atggtttcag ctgaatagcg acctagataa gttgcgtcta agatgaattt
    12121 attgtggatg atatcttcca actcagctgc acgaacatct gctggatttt caggatctag
    12181 aggatattta gtaggcaggg cgtgaacaac accaatttcc cctttatagc ctttctcttt
    12241 gtacaatttt accgcgcgtg catgagacac catcatattg tggtgtgatt gaaagacttt
    12301 ggcaaggtcg tactggatac ctggagggaa tttcccaacc aaatattgac catcaccgat
    12361 tggtccaatt tcattaaagg ttgtccaata gtttacttct ggaaattctt caaaacagaa
    12421 ggcagcgtag tctacaaaat gttcgatatt ttcacggttt aagaagtctc catttgagtg
    12481 gagagcttct ggcgtgtcaa agtgatgaag agttacaaaa ggctcaacat gacgtttatg
    12541 acactctgca aataaattat gataaaactc aacaccttta gcatttactt ggccgtaacc
    12601 agtcgggaaa atacgtgacc aagcaataga aattcgaata ccattgacac catactcttc
    12661 tgctagcttg aggtcaactg gatatcgatt gtaaaaatca ctagctggtt cggcagtgta
    12721 ccagtagtta tcctcaagat atttatccca agcaactggt ccttttccat cagtatgtgt
    12781 agcaccttct gcttgataag cagctgttgc gccaccgaaa ataaagtctt ttggaagtgt
    12841 ttttgtcatt tttttcacct atttcttgat ttaaataaag taaaagtgag aggagaggag
    12901 tcagtgagcc atgcttctcc atctcacttc ttgtaccaag tcactgaatt gtgacatgag
    12961 gaggtaaaaa cgatatcttt ttaccgacga attaataagg cgattaggaa attcgccata
    13021 gcgatttccg taacgatggg agactgtttc acagttccta gccttaatcg aattgctctt
    13081 gtacgaatgc tagggcacct tttccatcac gagttaattt gatgtattga gcgccttctg
    13141 tcttagcaag tttaataccg agcttatctg tttctgcctt catatcttca aagtttgaag
    13201 caacttgagg agcaaggata accaaatcaa actctggcaa catttcacgg tgagcaccat
    13261 agccgcctgc tgctgctttc acagggacat tgtattctgc tgctgcctta ttcaaagcat
    13321 ttgcaaggag accacttgta cctccacctg cacagagaac gaggacattt gtttcttcag
    13381 tgatggtatt ttgtgctgca tcgacacccg ctttttcaag aatagcatcc gcttttgcag
    13441 tgttgaagtt tgcagcaact ttttctttca attcagatga agagtttcct ttccgttctt
    13501 cttctaaaat ttgatggtca taaactttaa caaatggata atagattaaa gtgtcgataa
    13561 tgattaaagt tatagccaag acgaatgaca atggagccag cgccgttccc ataacaatac
    13621 caattggagc aggtgttgtc caaggtaaaa taatactgaa actattcatt tgtagaacat
    13681 ctacaaaaaa tttcataatc caaacattga taataggagc agtaacaaac ggaataaaga
    13741 agataggatt taaaacaatt ggagcaccaa ataggattgg ttcatttacg ccaaagaaag
    13801 taggaactac agacgcacgt ccgacaattc tatttctttt tgatttacat aaccacataa
    13861 acataaatgg aactactagt gttgcaccag taccacccat tgttacaaca aaagtttgag
    13921 taactggatt taaaatttta tctgcgtgca taccagcttg cacaagctga acattagctt
    13981 ctatatttgc ataactgata gctacaatta aaggttctac aacagatggc ccatgaattc
    14041 caacaaacca aaagaaagca taagcaccaa atacaatagc taaaccgata taaccatcag
    14101 tagctgaaaa tagaggggct aatattttac caacagattc tgctacattt gtaccaaaaa
    14161 tatttctaac aattaaatcc aatccatata ttgaaagaac agataaggca aacggaataa
    14221 catctttaaa aacttgtgca atatttggtg gaacctcttc aggaagtcta atggttacat
    14281 tatttttaat acatacttta tagatattta ctgtaataaa agctgccaaa aatgcagtaa
    14341 gtaatcctct agtacctaaa agaccattag caaatccacc ttcaattgga tcagctgcca
    14401 gtattaacaa tccaacaaca gctgctaaaa aagttgacat gtagttgatt tggttggttt
    14461 tctccatact acgtgtatta actgaatctg ttaacgattt tgcagttgtc ccagctacta
    14521 agagagctaa aatccccatt gagtagttgt aaggtttcaa aataaaagct aactgttcat
    14581 cagaccattt aaacccaaaa atattgggca cagcagctat tagtataaaa atacttgaga
    14641 ataatactgc tggcatactg gaaataaaac catcttttat cgctcttaag tatatatttc
    14701 tcgataattt ctcaaagaaa ggctttcctt tctcaataaa aattattaac ttatccattt
    14761 tatttaactc ctctcttgta aagttcaatt aaatggtgca ttaggtcttt taacaagata
    14821 gttgtcatca agtgatcttg accatgcatc atggtcacac tatacgctaa gtcctcacca
    14881 gaagcttcct tagtcaatag acttgtttgc gcgtggtgag cctctgcaat acagctacca
    14941 gcttcctcta ccagagcgtc cgctttttca aaatcaccag cttcagcagc cttcaaggct
    15001 tccaatagtt ttgaacgagc atcgccagca taggctacga tttcaaaacc taacaatgtt
    15061 acttcttctc tattcatgat agaattctcc ttatatagtt ttaaataaat ttttatgaca
    15121 ataaacgttg gatatgtaga actagataaa ctcgttcact tttatacaaa tcaagacccg
    15181 tatgttgcgt aatcacatca tagatcttgg aaccaatctc gaaggctttt ggataggatt
    15241 gtttaatatg atcttccata tccagaagtg attggttatc atctctagat ctgtctaaat
    15301 aatccaagaa ataattcaaa tggatcataa agcgatcata gaaatggtta ttctttttag
    15361 ttcgttgaat tgcatagtcc gttaaaactt cttcaacatt cctgagaatt tctttcctct
    15421 tatcaatcga ctccacaagt tccacttcat tttcaccttc agcattaatg aaatgataag
    15481 caatccgaat aatttcgtcc tcaggaaaat gatctgctag cttctgacgg taaatttcaa
    15541 aagcttcatt tgcgatttga aaagcgacag gatacttagc ggaaatatct ggcagattac
    15601 tatccttgta ccttccttga gttagagctt gataagaaca gtaaatatga tctgtcaagg
    15661 ttacatagag atactcttga atcggataat gatatttctt tgatagctta tcaatgattt
    15721 cataggtcac tgtgataaaa tcaagcggaa catctttgag aagagccata aagttttctc
    15781 tggactcttc ggtcttcatc cgaaagattt tctcaacctg attttcagca atcaaatccc
    15841 ccttcttctt tccgaatgca attcccttac caattacaat cacctcttct cccttatcat
    15901 ttctgacaag cgagacattg tgattcattg gatttagaat tcgatacatg tcaacctcct
    15961 tttatatctt aaaggtatat aagtacaaaa aatgaccaca aatgaactag aaaatcagct
    16021 gatttctaat tcacctgtga tcatgcctaa tattacttag taacactcac cttgtattaa
    16081 cttgtgattt aagtatagca caagtaagtt attttgtaaa cctttacata caactttttt
    16141 taaatttttt tagattcatg ttcctaaact aggaggactt tcttacacac gttctttcca
    16201 tgaagtcgct gttgtttgaa gaactttatt gagctcatca atgttttcaa atccagttgt
    16261 gcgaagccat tcgcgagctg ctgcttcacc atctttgatg taagcttcaa ctgatcctgc
    16321 ccatgtagca cggccacaaa gaactccgtt aaagtttgca cctgattcat gagcaaatac
    16381 aagagtatct tggaagagtt tagctgatac accagcactc aagtagatgt atggcaagtt
    16441 cgttgcttca tcttgcgctt tgaagaaggc tgctgcttct tcacgtgtat aaactacttc
    16501 accttcagcg aagccttcaa catatttaat gttaacagga acttcaactt tcaagacatc
    16561 aatgttaaag cgtgggtctg aaaagacttt catagcgccg ataactttgt gtggttttac
    16621 tttcgcgtat tctacagaac ctgcatccgc aattttttca tcgtaagcaa ggatttcaag
    16681 gaagaatggg atatcttcag ccacacactc agaaccgatg cgttcgatgt aggcttgttt
    16741 ttcttgattg agttcgtctg agctatctac atcatagtaa agcaagaatt taactgcatc
    16801 tgcaccttct tctttaatac gttttgcaga ccagacatcc aagcagtctg gcaagcgttt
    16861 tgtgcttgtt gtgtcataac ctgttttttc ataagcaagg agaagaccag ctttttcatc
    16921 aagagcttta gttgctggaa gtccatactc agggtcaaga agcatagatg aagcgtattt
    16981 agtcaattca tctgctacca agactttaag ttcttccatt tgggccacag ttggttcttc
    17041 tgtttggtgt tgagccatga ggcgtttcaa agcaccacgt tggtcaaatg caagagctga
    17101 gatgatacca ttttcatcag aaagtttttc taagcgtaca cgtttttgtt ctgttaaagc
    17161 cattttatac ctcttttact attaattgat catatagaac ttgatagttg gccatgttga
    17221 catgaccagt cattttttct tgagcattga gcataccaag gacatttgcc ttgatgagta
    17281 attctgcatc cgattctttg tgaagaagtc ctgaagaaat tcctgccaca gtagagtctc
    17341 cagatccaac aggatttacc acctgaattc taggaatatc taccttgtag aaagtgtcac
    17401 catgtttggc aaaagtaccg ttggcaccaa gtgaaacgat aatccattca atccctgcaa
    17461 acaaagggtc ttgaagtact tcttttaatt catccaaatc ctcagaaact tctcttccaa
    17521 gaagctgaga caattcttca ttatttggtt tgatgactgt tggtttatgg ggtgattcaa
    17581 gaacagcctg aagtgctgca cctgagcagt ccaagactac aggcttgcca gcttgattag
    17641 caagttctac caagctcgca tagtaatcaa ctggaaggcc agctggcaga ctacctgaga
    17701 tggccactac ttcaactgat tccaagagtt ttttgaaatg ttccaaaaac tcttgacctt
    17761 cctgttccaa tacttcagga cctttttcaa gaacttctgt ttggttgtct ccgtggagaa
    17821 tagcgataca gttacgagtt tctccctgaa ttgagaagaa atctttcttt acttgattat
    17881 cgatatgttc aaccaaaaac tcaccaagtt tgccacccac taaaccagta gcaagaacag
    17941 aatcaccaaa ttctgaaagt actcgggtaa cattgagtcc cttaccacca gccgttttgg
    18001 ttacatccac cacacgattg acagtatcaa tcttcaactc atccaaggga taggaaatat
    18061 caatggatgg gttcattgta actgttaaaa tcataggtta cctcttagtc gtggtattct
    18121 ccgcgatccc atttttcaag gaattcagtg aagaagtttg cgtctgtttg ttgagcattg
    18181 tgactttcat ggtgttcaat tttcgcaatc aattttttgt tttcttcaga tggtttgtat
    18241 tcagcatgaa tgaaagcttc gatgatatca cacataagca attcaccagt aatcttacca
    18301 ccaaaaccga taacgttggc gttcaattgt tctttagcat aaagggctgt tgtcatatca
    18361 cgaaccaagg cagaacgaac acctggaact ttatttacag cgttgttgat accaacacca
    18421 gtaccacaga tacatacccc gagatcagct tgaccactag ttactgcttc ccctactttt
    18481 ttaccaaaga ttgggtagtg agtccgtgta tggtcatagg taccaaagtc aatgacttca
    18541 tatccttttg atttcaaaaa ttctgaaacc gccatttttt catcagttac gatgtggtca
    18601 catccaattg caattctcat ttttaactta cctttcttac tgtaatctaa ttagcccatt
    18661 ttgttcaaca tgtcaacccg gatttgatga cgtccaccgt cgtatttacc attaacaaat
    18721 cctttagcga tgtttttagc caattcatca ccaacaagtt gtgcccccat agtgatcata
    18781 cgtgagttgt tgtggccacg agtcatataa gctgaacgtt cgtcagatac ttctgcagca
    18841 accattcctt tgatcttagt tgcaaccata aatggaccag ctccataagc atcaatcacg
    18901 ataccaaggt tttgttcttc tttgtttact tctgcagcaa cagcgagagt cacatcaaca
    18961 aagtcttgac cttcagctgt aacatccaca aggtggaagt tttctttttc taagaagtct
    19021 ttcacaactt ctttcaatct caaacctgca gcatctgcac cgataacaat agacatattg
    19081 tatactcctt tttatttttt ctatgctgga ttccagcagt gtattggctg tctttaaaaa
    19141 acagtcaatt agtcctcaca aacataatat actactattg tttgaagaag tcaatagttt
    19201 gtgtttgttt ttgttattta ttttttactt ttttataaaa cttctatctc aaaccctaat
    19261 tcttcaacct gattggcttc taagagacaa acattcttct tatcttctaa atgatctcct
    19321 tcttcaagga atgttgacaa gccagaccat ggttcaaagg taatgaaagg tcccttgttc
    19381 aaagttgacc agatgatgag gtttggaaat tctgcaaaat gcactttcaa tcccttatca
    19441 tgtttaagag aacgaagggc aattgttcga gattgcaatt catccaaagt cactgcatct
    19501 gtgctgaaaa gatcataact aagatctatt tctttttgtc cctctagcca tggacttcta
    19561 tcttggaaat ccaacatacc tgtttctggg aaaggacgtg gaacagagca agtctcttct
    19621 ttctcagact ctagataata atcttcatag actccatcat caagtagagg acaattaaat
    19681 cctggatgtc caccaataaa gtaaggcatg accttgctag tttctttatt agacttcctg
    19741 caaaactaga atcctagttc atgattgata ataccagcac tcaaattcat tcgtaatccg
    19801 aagcgtttac gatgatttcg ataggttgtt gaaaacattt taaacgtttt tactttggca
    19861 aagatgttct caaccttgct tctctcctta gatagcgcat ggttataggc tttatcttca
    19921 gctattagcg acttgagttt gctggattta cgtggagttt gtacttgagg atatatcttc
    19981 atgagtcctt gataaccact gtcagccaag attttaccag cttgtccgat atttctgcaa
    20041 ctcattttga acaacttcat atcatgacaa tagttcacag tgatatccaa agaaacaatt
    20101 ctcccttgac ttgtgacaat cgcttgagcc ttcatagcgt ggcatttctt tttaccagaa
    20161 tcattcgcta attctttttt taggtcgatt gatttgtact tccgtcgcat caatcattac
    20221 cgtgtcctca gagctgagag gagttcttga aaccgtaaca ccactttgaa caagagttac
    20281 ttcaacccat tggctccgac ggataaagtt gctttcgtga ataccaaaat cagccgcaat
    20341 ttgttcataa gttcgatatt ctcgcactat tgaagagtgg ccataagaag gtcttctagg
    20401 cttaatttag gttttcgtcc accttttgcg tgtttaagtt gataagctgt ttttaataca
    20461 gctaacatct cttcaaaagt ggtacgctga acaccaacaa gacgcttaaa tcgtgcatca
    20521 gttagttgtt tacttgcttc atcattcata gaactactat accatatttt gtttcgcagg
    20581 aagtataata ttgtcaaata ctggaacgct cattgctggg atacagaata agattgg
//
LOCUS       DBERCA010000029        20604 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.029, whole genome shotgun sequence.
ACCESSION   DBERCA010000029 DBERCA010000000
VERSION     DBERCA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.029"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(420..2147)
                     /gene="ezrA"
                     /locus_tag="ACL719_001232"
     CDS             complement(420..2147)
                     /gene="ezrA"
                     /locus_tag="ACL719_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGS0699194.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2229..4175)
                     /gene="gyrB"
                     /locus_tag="ACL719_001233"
     CDS             complement(2229..4175)
                     /gene="gyrB"
                     /locus_tag="ACL719_001233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGS0699195.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4190..4753)
                     /locus_tag="ACL719_001234"
     CDS             complement(4190..4753)
                     /locus_tag="ACL719_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0699196.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4838..5392)
                     /locus_tag="ACL719_001235"
     CDS             complement(4838..5392)
                     /locus_tag="ACL719_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGS0699197.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5403..6626)
                     /locus_tag="ACL719_001236"
     CDS             complement(5403..6626)
                     /locus_tag="ACL719_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS0699198.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6663..9113)
                     /locus_tag="ACL719_001237"
     CDS             complement(6663..9113)
                     /locus_tag="ACL719_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGS0699199.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9313..10242)
                     /locus_tag="ACL719_001238"
     CDS             complement(9313..10242)
                     /locus_tag="ACL719_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGS0699200.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10382..10669
                     /locus_tag="ACL719_001239"
     CDS             10382..10669
                     /locus_tag="ACL719_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGS0699201.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10703..12037)
                     /gene="ciaH"
                     /locus_tag="ACL719_001240"
     CDS             complement(10703..12037)
                     /gene="ciaH"
                     /locus_tag="ACL719_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGS0699202.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12027..12701)
                     /gene="ciaR"
                     /locus_tag="ACL719_001241"
     CDS             complement(12027..12701)
                     /gene="ciaR"
                     /locus_tag="ACL719_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGS0699203.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12810..15356)
                     /locus_tag="ACL719_001242"
     CDS             complement(12810..15356)
                     /locus_tag="ACL719_001242"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGS0699204.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQHLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15479..15919)
                     /locus_tag="ACL719_001243"
     CDS             complement(15479..15919)
                     /locus_tag="ACL719_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGS0699205.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15894..16763)
                     /locus_tag="ACL719_001244"
     CDS             complement(15894..16763)
                     /locus_tag="ACL719_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGS0699206.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16741..17241)
                     /locus_tag="ACL719_001245"
     CDS             complement(16741..17241)
                     /locus_tag="ACL719_001245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0699207.1"
                     /translation="MEIKKHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETRFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17252..17950)
                     /locus_tag="ACL719_001246"
     CDS             complement(17252..17950)
                     /locus_tag="ACL719_001246"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGS0699208.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17943..18179)
                     /locus_tag="ACL719_001247"
     CDS             complement(17943..18179)
                     /locus_tag="ACL719_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGS0699209.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18279..19205)
                     /locus_tag="ACL719_001248"
     CDS             complement(18279..19205)
                     /locus_tag="ACL719_001248"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGS0699210.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19280..20095)
                     /locus_tag="ACL719_001249"
     CDS             complement(19280..20095)
                     /locus_tag="ACL719_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699211.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20099..20419)
                     /locus_tag="ACL719_001250"
     CDS             complement(20099..20419)
                     /locus_tag="ACL719_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS0699212.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6189 a         4525 c         3660 g         6230 t
ORIGIN      
        1 ggcctattta ccgtgggact aaagttgtac aagaaaagtg caaataagaa atctccagat
       61 taggaactat ccgtgagttc tctagtctgg agatttttca atagacttcg ttattggacg
      121 gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt
      181 aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt acttgagtac
      241 ggtaaggcga cgctgacgtg atttgaagag attttcgaag agtataaaac actgttttga
      301 ggtggtggat aaatcggctg ggtaaaaact caatctcagg agaaaatgaa gtaaatctcc
      361 ccacaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt tttcttttat
      421 taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt ggttacacca
      481 ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata atcaaattct
      541 ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc aaatgagcga
      601 tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac aatattataa
      661 gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg ggtaacagat
      721 tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt attgcttgcc
      781 gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt ttccatgtat
      841 cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc attaatatca
      901 tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc aatatctttt
      961 agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg ggttggttct
     1021 tcttgattcg aagttacctc aacaatagct gcctcaaaac tctctaattc tgtctgaata
     1081 cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa acgtgcaata
     1141 tcttctccca ataaagtatt attctctttc atatgttgaa ggtaagttgg aagagttgca
     1201 agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc atacaaggca
     1261 ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc caactgacga
     1321 atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc ttcaatatcc
     1381 gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggcttc caaatcctgt
     1441 aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg atccacaata
     1501 tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc ttccacaggg
     1561 tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt ttctaattgt
     1621 ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac tctatgctga
     1681 agttcctcaa ataaatccaa agcatgaaga acacgaccac tatttttaga ttcttgcttc
     1741 tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa agtaatttga
     1801 ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg gttatagcct
     1861 tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa atcgacccat
     1921 ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat attttttaca
     1981 gcttctactt catcatttac tggaagattg tatagttctt cttttctttc ttctagcgcc
     2041 tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc cagaactaaa
     2101 atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc aaactccttt
     2161 tttacttgaa acaatcgtaa cgattatatc atatttttta gaccaaggga actacttctc
     2221 cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa ctcacgacga
     2281 ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc agcatcatct
     2341 acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca cagctgatga
     2401 tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg accttcacta
     2461 taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggctg gatatattct
     2521 ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata aacataacca
     2581 gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt acgaatgtgg
     2641 gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc tttcgaaaca
     2701 tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat ttcttcgtta
     2761 gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg aattggaagg
     2821 atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga gtctccttcg
     2881 acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag tttccctgga
     2941 aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg cttggcagcc
     3001 acacgagcct tggcagctaa aatccctttt tccacgatac gtttggcaat ctgtggattt
     3061 tccatgagga aatcggagaa ggcttcactg aagaggcgat tggtaatctt gaccacttcg
     3121 ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg tttaactgag
     3181 ataactgcag ttaagccttc gcgaacatct tcccctgtta ggttgtcttc attgtctttc
     3241 agtaacttat ttttacgagc ataatcattg ataacacgtg tcaaggctgt acggaaacct
     3301 tgttcatgcg ttccaccttc atgtgtatga atattattgg cgaaactcat gacattttca
     3361 tggtaaccag ttgtgtactg catggctacc tcaactgtga tatcatccat ctcaccgtct
     3421 gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc aacgtaacta
     3481 gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttcacg cttatctgta
     3541 attgaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt atttaattta
     3601 tcaaaatcaa agattgttgt ttcagtgaag atttttgggt ccggtgtgaa gtgaacagtt
     3661 gttcctgttt tatccgtatc tccaactatt tcaagatctg cgacaacatg accacgacgg
     3721 tattcttggt aatgaatctt accatttttg tgaacatgaa cgtctaattg agtggaaagg
     3781 gcattaacta ctgacgaccc caccccgtga agaccacctg aaaccttgta tccaccaccg
     3841 ccgaactttc ctccagcgtg aaggactgta aagacggtct caacagcagg acggcctgtt
     3901 ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat cgaatcatct
     3961 ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc aattgagtta
     4021 tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc aatgtacatc
     4081 cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact ggcatcataa
     4141 tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct tatatgtcta
     4201 atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct gcagcttgac
     4261 ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc tgatactttt
     4321 ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag ctagaagtca
     4381 ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa acctgatcat
     4441 ttcgatgaga gaccaaaaaa tgacggccac cttgatttga aatgacttcc aataggtcag
     4501 aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc ttgtactttt
     4561 ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta gaaaccttta
     4621 aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat gtttcaacaa
     4681 atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc cagatgtaat
     4741 cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat gatttccctg
     4801 agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta ccaaagacat
     4861 cttgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc tccaccaact
     4921 cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga ccatctacca
     4981 ctactgtttc cttgacatag tgaccatcaa ggatttgctc ttgaacgccg tcataacaag
     5041 tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc gcacaaatgg
     5101 ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag gtctgattat
     5161 cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca tagtctgata
     5221 aatctccatc aaagacatga tctgctctta cttggattgc atgcgaaccc gttacttgct
     5281 cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct acaactgtca
     5341 aggcttcaat ttcttcaaag ccctgagcta acataactgc tacttttacc atgattttct
     5401 ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt tccgctcttt
     5461 tcttcagcta gattagtctg gtaactcatc gataaaagca aaccaacacc aatcagatta
     5521 ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt caaaggaagt
     5581 agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat aatcaaacct
     5641 gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa catacggtaa
     5701 atcaacatga gatagagggc aataaccagg acagagccaa taaagccaaa atcttctgca
     5761 ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga agcattaaat
     5821 ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg ctggtaagtc
     5881 gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattaat ttggtaggtc
     5941 ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat agctaagaaa
     6001 ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca agaaacccct
     6061 gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc caagtcactt
     6121 tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa aattaacaaa
     6181 aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg gacaatgaca
     6241 cgagccaaca tgaggatata ggatatcttc ataaattctg acggttggaa tagggtaatt
     6301 ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaagcttgg attataaaat
     6361 acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt caccttccaa
     6421 agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa agcaatccag
     6481 gcgacctgct gccctaaaat gggcagaata ttattgggat aatcatgact aacggctata
     6541 tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa actgtaatcg
     6601 actcttgagt cgagagaacg tttcatatat actaacctta tactttcata caatactatt
     6661 tatcaaagtt cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg agagatggtt
     6721 ttaacagtcg cttcttctgc tagagatgct actatttgtt tgccgtatcg ttttccgacg
     6781 attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat acttcttccc
     6841 aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa ggcatttttc
     6901 ccttcttgat tcagttcttg attaatcttt ttcgtcaagg gttcttgagg attttggaaa
     6961 ggaagcctcg gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac tccctcccag
     7021 aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt ctttagctga
     7081 tgaacatccc catttttata ctgggccaag tggctaactg taagtaaatc cgatactgct
     7141 agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc ttgaatttcc
     7201 actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt taccagaggg
     7261 aaatctttga ccatgaccac ttcttgttcc tgttttcccc gagattcaat cttgacaaat
     7321 ttggcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat ctcaagagta
     7381 gctgatactc ccaagacttg gcaactctca ggaagtaaag aggacagaag agtacggcct
     7441 gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc aagccagtaa
     7501 tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagc cttccacttc taattctgaa
     7561 aaatactgat ggagattgtc cagagaatct aagatatttt tcctagattt gccagactga
     7621 aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg ttgttgaacc
     7681 ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg tatatcgtag
     7741 gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc aataatcagt
     7801 aaacggtcac tgacaaattc aggattatct tcaagtctgg tcacgagata ggcatgatta
     7861 gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa atcttccgtc
     7921 acaaataagc tctgggatga taaattccca tcatgacgaa ggtctgctag aaaatgttgg
     7981 taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt aagccagacc
     8041 aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg caaggaatga
     8101 taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat atctgtatgg
     8161 aacacttcct tgagacgttt accttcttct tccatgattt gattttgaag aatctttgtc
     8221 ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa gagatagcca
     8281 taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc tttcaatagc
     8341 aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt caacagagaa
     8401 atatttttag aaaagtcttg agatagtttt cgtagctcca gagaagctgc cgttttctta
     8461 aaatacagac cttgaacttg gaccaagtct ggagaactca ggatagattg gttgcgataa
     8521 gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc cagcaagcgt
     8581 tccaagagac ctttaggaag ctgggtcatc ttttcccgta aaaaaagaag taattctgct
     8641 gtagcttggg catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc taattctcga
     8701 cacaaaatcg gcaagctata tttttccagt tcagggaaaa agacctgggc caattcgacc
     8761 gtatcaacac gagggtttct tagctcatag ccttcaaaaa ataaattttc cgccaagaga
     8821 ttagcatcaa actgaacatt atgggctaca aaaatcccat cctccaccaa gtcaaatatt
     8881 tttctggcaa cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt caatcctgtc
     8941 agttctttga tatgagcatc caagggttca tgtggattga catccgtcgt atagtgatcg
     9001 acgatttctc cgtcctcaat cacgacaatt cccacttgga taattttagc cttactacct
     9061 gtgctagttg cctctaaatc caccacaaca tagcgattac caatcgtttt cattataaaa
     9121 aattatacca aaaaaagggc catttggcac ctgcaaacat aggataattt tcaaactcaa
     9181 gaacactact tttcttaaaa atcccatcga atagagcatt ctagcctgct gatttttgta
     9241 aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt tgcatcttcc ttctctcaac
     9301 gaatcatgta ttttatctaa ccatgtcagg atcgtagtca taaaatcctg tatcaaggaa
     9361 acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt gatcatggct
     9421 aaagttgccc tcttgaatca aattacgcgc ttggataaag agttttgcaa tctcaacaaa
     9481 gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat gctccaccaa
     9541 ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg tcccatgatt
     9601 atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga agaggtcacg
     9661 tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca agagtggacg
     9721 tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa tttccaaaat
     9781 ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag cctcagatgc
     9841 gccagccttt tgcccccaga agttaatctg acgactgcta gttaccatgg ttgaagcatc
     9901 gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc ctgcatcccc
     9961 aatcgtaata tagtctgtct tgatttcttc caagaagtct aagaaaggct tgatacggtc
    10021 catcatatct tggtgcatga gagcattgac cgcaacgagc aattccttac cagcatcatg
    10081 aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca gacgaaggcc
    10141 aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt gttcaacttg
    10201 ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa ttataccaca
    10261 tttctccaaa atcagccatg ctttgaaaat gaaaagggac tttattcttt ttgtaacctt
    10321 tctgtaataa ttctctgccg aaaaaatgat aaaataggta tgtactgtta aggagagaat
    10381 catgcccgta agaaaattac aatcctatga ggtagactat caagaagaat taaaccagca
    10441 gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc tcaaggaaat
    10501 cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta gttttatctt
    10561 tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat tcagcttact
    10621 tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa tccaaaatat
    10681 cgccccaatt ggagcgatat ttttattttt tctttttaga tggtgtctga atggcaatct
    10741 tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt ccttttagag
    10801 catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt tgccgggttc
    10861 tagctttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct gtcgaaatac
    10921 cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag ataagaaaat
    10981 caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg gtcatcagtt
    11041 gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca aaacggaaga
    11101 cacgattatt ttccgaagca atcatctcgt agtttgtgaa agttgtatta aaaaagctag
    11161 ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag ttcagcaagc
    11221 ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg ctttcgctca
    11281 catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt tgcaaaactg
    11341 cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc ttctgcatac
    11401 tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt aaagacaaaa
    11461 tccagaaact agccatcacg accacaatca attgctcatg cttttgacta gcctgctcca
    11521 actgactggt attaatcaag acagcagcat actttatatt ggttgaaacc gaactaatat
    11581 tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta aaaacctgaa
    11641 tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag cccaaaaatc
    11701 gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc gtattggaac
    11761 tgacattagg ttttatttct actttactag cgtccgccct agcattttct aaatctttaa
    11821 tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt tcactcagtc
    11881 catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt aaaataatca
    11941 gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa taactaaagt
    12001 catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata cccaacacta
    12061 cgcaaggttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg gacttttgaa
    12121 acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag acggtcaaaa
    12181 atctgcgtct taggcaaaat cacattttga ttttgaagga aataaactag taaatcgaac
    12241 tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt tgataaatta
    12301 accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg tttgagaagg
    12361 gcctgaatcc gcattttaag ttcttctagg tagaaaggtt tggtcagata atcatccgct
    12421 cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat aatcagaact
    12481 ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt tttttctggc
    12541 aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta gagaccttct
    12601 tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa tactgaattt
    12661 gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc ctccttatta
    12721 aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt acatgagata
    12781 gctgagtttt ctttttattt taggcttatt tatgcatttc cgtattgaag aacaactgct
    12841 tcgactgcag ctttttcacg gctaatcaag tcaacacgcg ctgcaatttc cttgattccc
    12901 ataccgatgt tacggctaag agcaaggtca gaaagttgcg gctcaaagaa ctccttgtat
    12961 tccgccaagt gttgctgagt cttaaataca tgagcaggaa ggataacaaa gctatcaaag
    13021 ctcatatctc ctccaagggc tgccttaatc caagcccagt tttcacgcgc ccaagaccaa
    13081 gctgttttct gagttgcttg atgagctagg aattggtaat accaagcaga caagtcctgt
    13141 ggtttgacca caaatttgtc cttccaagaa gtaatcaagt tttggatatt atccgcatct
    13201 gtactgtatg caagagctgc tgccaactgg cgtttaaaga cagcatctgt tgcgtgagta
    13261 taagtatcaa gataaagtgc taacaagtct ttagtctcat gatgtttcat ctcattaatc
    13321 agaacttgtg aacgaatagc tgctgggagt cctgcaagat tctccttgtg tgttgcgaag
    13381 atttggctag cgacttgact agcttctgca tcatttgagc gaatcatcat cgaaacagcc
    13441 agctgacgaa ccaattcatc ctcatctgat tctccgtctt tagcttcaaa accaagacgg
    13501 tcatagttat gacgagccaa tttagcaacc agtcctttga aggctgtttc agcatccgtt
    13561 ccttcatcaa taaagcgctc aagggctgaa atcacttgag aaacagctga aaccaccaga
    13621 taagactctt ccttagcaag tttatcaaga actggaagca agtctgcata agaaatgtgc
    13681 cctgcctcag ccaacaaacg acgttcttga acaatttgca gtttgcttgt gttatcaagt
    13741 gtctctagct cagcaagaac agcttctaac aagtctcctt gatagtcggt aatatagtgg
    13801 gcagtatttt cagtgttgag acgaagagct ccttcatttt cagcaagaag agctgcgtag
    13861 ccagggattt cgatactttc agtttcgagt gtatcaggca agcctttcca gttgctattg
    13921 aggggcacca cccagagacg gttcttgtct tcgttctcac cgatgaagaa ttgtttttgt
    13981 gaaatcttca agacatcatt ttcaacttta acagtaagaa ctgggtaacc aggctgttcc
    14041 aaccaagaat ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag ggcatcccaa
    14101 aggtcactac caatggtgtt gctgtattgg tgtttttcaa agtaggcgtg caaaccttta
    14161 gcaaaatcag catctcctag ccaacggcga agcatgtgca tgagacggct tcctttggca
    14221 tagacgatag cgccgtcaaa gagtgtattg atttcatctg gatgtttaac ttcgacgtgg
    14281 acagactgaa cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc acctgtttgg
    14341 aaatcttcaa agatattcca gcttggttcg atggtatcca cacagacgta ttccatcata
    14401 ttagcgaaac tttcattgag ccaaaggtca tcccaccatt tcatagtcac gaggttccca
    14461 aaccattggt gagccaattc atgggccaca acaagggcaa cttgttgacg gctagcaaat
    14521 gtagagttct catcgacaac caagtaaact tcacggtagg tcacaagacc ccagttttcc
    14581 atagcaccag ctgagaagtc aggaagggcg atgtggagag attgaggaat tgggtactta
    14641 actccatagt aatcttcgta aaactcgata gagcgaacag cgatatccag tgagaaatca
    14701 agatttgaaa gtggatgtgc tttggttgag tagacaccta ccagggtacc atttttagtt
    14761 ttagcggtca ccccttgcaa atcaccagca acaaaggcca acaagtaaga agacatgcga
    14821 ggtgttgtct caaacttcca gatacctgtt tccttacggt tttcaacatc gatttctggc
    14881 atgtttgaca aggccaattc accttctgct tggtcaaagc gaagagagag gtcaaaagtt
    14941 gctttggctt caggctcatc cacacatggg aaagcttcgc gcgcaaaatg gctctcgaac
    15001 tgagtagaca agacctcctt cttgactcca tcaactgtat aataagaagg gtaaatccct
    15061 gtcatgttgt ctgtaatttt accagaaaag gcaagaacca attcaacttg accagcctca
    15121 gccaattcga tatgaagggc ttcattgtca tggtcaactg taaatggacg agcttgacct
    15181 gcaacttcta cagaggtgat ttccaaatct ttttggtgga gggagatgcg gtcactctgt
    15241 gcttgaccag tgatggtcac tttcccagaa aaagtcttgg tctcacgact caaatctaaa
    15301 aataaatcat aatgttcagg aacaaattgc ttaataaaat gttcaactgc ttgcatagtt
    15361 ttctcctatt ctaagtttaa gagctgctgc tcttcttcaa acaaacttat tatatcatat
    15421 tttgcttgag aaaaaaggat tggacagcga tttccaaaac tttcttcctt ctaatagtct
    15481 acagtgggta gaccttgcga aattcttcta aaacaacctt gctttcaggt gtaaaagtca
    15541 atcctgcttc acccaagcag tctctgaaaa aatcctcatg aacctggcgc caatagacaa
    15601 gtgtcttgtt tccctcacct tccttatagg catggtcagc tgacactcga ttgaagggtt
    15661 taacagaaac ctttgtaatt tcgacaatgc agacagcctg attttgacta tctaaaatga
    15721 catcgaaggt ccctacttgg ggaagtggtt cgtcttctag tacatagagg tcataggctg
    15781 atgctgttgc tgtcttttct cctttaaaca ccaaatccgc taaaaggtct ggttcaactc
    15841 caaaagccca ggcatcgatt tcatctccga tagaaggatt gatttgtttg tatttattcc
    15901 acatttcttg cggtatcatg ggtactcctt tgtaattttt tactttcttc ttttatgtgt
    15961 ttaagatgat ctggatggtc aatctctaaa tcaaaaatct ctggaataga actgtagtgg
    16021 ataatgcact tgatacccaa ctgattcatt ttttgtatga aagaagtatt cagatagcct
    16081 gctacagcaa aattaatctt gttctttctt gctttatcct gcatatctct tagcatatct
    16141 aacattattg gactttccat atcatgccat tgactgtttc tcatagtcgc aaaaacaaag
    16201 gaagtcaaat cattcattcc aactacaatc tttgaaatgc ccgtttccag tatactagat
    16261 aagtcaaaat acgctgacgg taattcaatc atcgttccga ctttcccagt aaaaccctgc
    16321 tgacgcaata ctgtaatagc ttgttttaat tggtcgtcat cattgacaaa aggaaagata
    16381 acagatagat tggggttggt ttgataaact tctgtaacga catgtgcttc agcctgaaat
    16441 tcatccaaac acgccagtaa acgcctggtt cctctatagc caaacaaggg atgcccttcg
    16501 tcaaaaaact ctctagtccc cactaaacaa ttggcttctg tattcgttaa ttcagaaaaa
    16561 cgataccaaa cttcttcatc tgagtacagg gagcagatag tctctagata atcttttaca
    16621 aattgctgac aacttgctaa tatgatgttt tgattaagct ctctcaacaa atattcccca
    16681 cgaatcatgc cgacgtggtg aaatagttga ggataaattt tttcaagaat tttttcgcca
    16741 ctaagggcaa gttgatttct catcattcac cttccaattc atgtaagaag tcttgtccag
    16801 ttctggaaat cctaataatt cagacttaac cttcaagact aatggcgatg cattttcttc
    16861 tgtaatttct tgaatatcca tccaaatata tccaagtgaa tcattcgcac catcagacac
    16921 agcttccgaa gtcgtaactt gaggtgcact ctcattcatt tcaacatcat acaaggccat
    16981 gacatggtga accataaaat tttttaactc ttctctgacg aaaacatcgt agattcgagg
    17041 attagagtag cttctaacag taaatctcgt ctcttccata acttctctag tcagcgtttc
    17101 cgtcagtcct tcaccaagtt gctgactgcc tccaggtaga tcataccgat gttgataagg
    17161 gcctctcgtt ttttcaatgc aaagtaactt tccattttca aagcaaacac agtagacccc
    17221 aaagtgtttt ttgatttcca tccaactcct cctacttcaa agaccagcca ccatctattg
    17281 tcaagatttg tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga ctaagctctg
    17341 ctatttcctc tggctcaatc cagcgcttga ttggggtttc actagccacc cagtcagcca
    17401 aaccacctgg ttcaaaatcc gcagcggtca tagctgtctt gactgctcct ggagcgatac
    17461 caaagacctg aatcccagct tcagcatagt ctagagccaa ctgcttggtg aagccagcca
    17521 aggcatgctt ggatgaagta taggcgtgac cacctccacc tgctaggcta gaagcaatgg
    17581 aacacatatt gatgatgatt ccctttttat tttccagcat ttgtgtcaaa taataccgag
    17641 tcaactctac tggaataatg tagttgattt caaaaatctc ttgaatgtcc tgcgccgttt
    17701 gttccaacag tggtttgtaa tcatccaaaa ctccggcagt attacacaaa acatccacct
    17761 gagggcacca gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt aaaaagtgaa
    17821 aatcaccctc taagagtggc ttttcacctt ggtcaactcc ataaacttga tagcccttct
    17881 ctaaaaagag gcgagcttga gccaatccga tccctgaact cactcctgta atgagtacac
    17941 gtttagtcat gcacttctac ccaatccgtt gccaaaacat cacaagctgt cgggctccac
    18001 atggaaaaac cttctccttc tccagacacg ttgattagga aataaggtgt cacttcaagt
    18061 gcaagcccat tttgctcgat ggtatcaaag agttggacat agttttccgc acctccccaa
    18121 ccagttcgta catatttttt cttagccttt aagccaggta aaatttcttc aaatgtcatg
    18181 tttttctcct ttaatgtcaa taattctccc cttcattata acaaaaaaac cgctttaaaa
    18241 cggctttttg actgtgattt attcaaacct gctactactt acggcaaatt attccctgca
    18301 gcaagataaa tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact
    18361 tctctcaagt gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc
    18421 aatatccaag aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca
    18481 agtacttgat ttaaagcttg aaatttctca tttccaacaa agttcccttt aaaatacccg
    18541 aattgcaaga cagaccatgc ttgaatgacc acatcgtgta atttgcaata ttcaaaaatg
    18601 ctgccatctc gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat
    18661 cctggagtaa aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct
    18721 tttttaagca actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta
    18781 ccttgtttat aaaggagatc aaaggcttct gctacttggt cagattccat caaagcatct
    18841 ggtcgatgaa ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct
    18901 actgatttta taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg
    18961 ccacatttgg actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga
    19021 cctaacagtt cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt
    19081 ccaacagaaa gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt
    19141 ctcatcattc cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat
    19201 ctcatcaaat ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg
    19261 ctttgcaacg gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca
    19321 agacaaagat tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg
    19381 aaattcccat ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca
    19441 tactgacaaa tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa
    19501 tcgtaaagac agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga
    19561 agcttgccat tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt
    19621 aagtgccaat tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca
    19681 caagtccaaa tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg
    19741 ttaagagagg acctttagaa aaatcactta gtagttgata ataacgtaat accgccagga
    19801 caagaattgg cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct
    19861 ctttcattat ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt
    19921 ctgcttctga gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat
    19981 agagaaatcc agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct
    20041 cagaaaactc atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt
    20101 aacccccatt cttaatccct tctacttttt gactcaaatc gtcccattgt tgccaaaaga
    20161 ctgagacacg ctcttctcct tctttcatta atgaaaaata cttccgatct ggaccatctg
    20221 gcgacgggcg catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat
    20281 aaatagttcc tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat
    20341 aaccataccg ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta
    20401 atagctgagt ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa
    20461 cttcacctat agtatatcac ttctacacta gtttgtaaag cataatagtt aatactcttc
    20521 gaaaatctct tcaaaccacg tcagcgtcgc cctaccgtat gtagggttac tgacttcgtc
    20581 agtttcatct acaacctcaa aact
//
LOCUS       DBERCA010000030        20561 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.030, whole genome shotgun sequence.
ACCESSION   DBERCA010000030 DBERCA010000000
VERSION     DBERCA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.030"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            40..159
                     /locus_tag="ACL719_001251"
     CDS             40..159
                     /locus_tag="ACL719_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699213.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLI"
     gene            178..1362
                     /locus_tag="ACL719_001252"
     CDS             178..1362
                     /locus_tag="ACL719_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0699214.1"
                     /translation="MLISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAY
                     RLSEGYGTDGPSAGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSL
                     FMAITIGLVTAEIYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGL
                     EAAGVAGGLNGLLGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDP
                     VWLQFTTENQEAVAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKT
                     LGKLAIIPSIFNINTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTG
                     VILPWTMPPIIGGFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVG
                     GK"
     gene            1364..3250
                     /locus_tag="ACL719_001253"
     CDS             1364..3250
                     /locus_tag="ACL719_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HGS0699215.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3418..3762
                     /locus_tag="ACL719_001254"
     CDS             3418..3762
                     /locus_tag="ACL719_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0699216.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3772..5184
                     /gene="lacG"
                     /locus_tag="ACL719_001255"
     CDS             3772..5184
                     /gene="lacG"
                     /locus_tag="ACL719_001255"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS0699217.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5254..6933
                     /locus_tag="ACL719_001256"
     CDS             5254..6933
                     /locus_tag="ACL719_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS0699218.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(7059..8498)
                     /locus_tag="ACL719_001257"
     CDS             complement(7059..8498)
                     /locus_tag="ACL719_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS0699219.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(8502..9851)
                     /gene="trkA"
                     /locus_tag="ACL719_001258"
     CDS             complement(8502..9851)
                     /gene="trkA"
                     /locus_tag="ACL719_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGS0699220.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            10040..10888
                     /locus_tag="ACL719_001259"
     CDS             10040..10888
                     /locus_tag="ACL719_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGS0699221.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10903..11451
                     /locus_tag="ACL719_001260"
     CDS             10903..11451
                     /locus_tag="ACL719_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGS0699222.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            11458..11670
                     /locus_tag="ACL719_001261"
     CDS             11458..11670
                     /locus_tag="ACL719_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699223.1"
                     /translation="MKIKEQTRKQVAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            11772..13454
                     /locus_tag="ACL719_001262"
     CDS             11772..13454
                     /locus_tag="ACL719_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699224.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            13439..14269
                     /locus_tag="ACL719_001263"
     CDS             13439..14269
                     /locus_tag="ACL719_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0699225.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            14462..14965
                     /locus_tag="ACL719_001264"
     CDS             14462..14965
                     /locus_tag="ACL719_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGS0699226.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      15019..15253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15418..15981
                     /locus_tag="ACL719_001265"
     CDS             15418..15981
                     /locus_tag="ACL719_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0699227.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            15971..16621
                     /locus_tag="ACL719_001266"
     CDS             15971..16621
                     /locus_tag="ACL719_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS0699228.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIR
                     GDLPDYLTILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            16649..17038
                     /locus_tag="ACL719_001267"
     CDS             16649..17038
                     /locus_tag="ACL719_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGS0699229.1"
                     /translation="MKVNIADLHLTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            17043..17492
                     /locus_tag="ACL719_001268"
     CDS             17043..17492
                     /locus_tag="ACL719_001268"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699230.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            17620..18207
                     /gene="rpoE"
                     /locus_tag="ACL719_001269"
     CDS             17620..18207
                     /gene="rpoE"
                     /locus_tag="ACL719_001269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGS0699231.1"
                     /translation="MKLEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVTPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            18597..20204
                     /locus_tag="ACL719_001270"
     CDS             18597..20204
                     /locus_tag="ACL719_001270"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGS0699232.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6092 a         3536 c         4574 g         6359 t
ORIGIN      
        1 atatttaatt tataaaaaat aaaaaaagga gttccggtga tgaacaatat tttagcgttt
       61 ttagaaacaa aagtcgctcc gtttggtgaa aaagttggca accaacggca tttgaaagct
      121 attcgtgaag gatttatgat ggcaatgcct ttgatttgag tcggatcttt atttcttatt
      181 ctaatcagtt ggcctcaaga ggattttaca aattggctga atagtgttgg attgctaagt
      241 atcttgacaa ctatgaatca gtcaacagta gcgattatct ccttggtcgc ttgtttcggt
      301 attgcctaca ggttgtcgga aggatatggt acagatggtc cgtcggcagg gatcatagcc
      361 ttatccagtt ttgtattgat ggcacctcgt ttttcgagta tggtttatga taaaaatggg
      421 gagcaggtca agcagttatt tggcggcgca ataccatttt ctagcctgaa tgcatcttct
      481 ttgtttatgg cgattactat tggattggtt acagcagaga tttatcgtat gtttatccag
      541 cgcggaatta cgataaaaat gccaagtggt gtcccagatg tggtaagtaa atcattttca
      601 gctcttttac ctggctttac tacttttgtt ttgtgggctc tggtcttaaa aggtcttgaa
      661 gcggcaggag ttgcaggagg tctcaacgga ctcctaggtg caattgttgg aacaccgctt
      721 aagttaattg caggaacgct tccaggtatg attctatgtg ttattgtaaa ctcattcttt
      781 tggttctgtg gagttaatgg gggacaagtt ttaaatgctt ttgtagatcc agtttggtta
      841 caatttacta cagaaaacca agaagctgtg gctgcaggac aaacactcca acacattatt
      901 acattaccgt ttaaagattt atttgtattt attggtggcg gtggagcgac tattggtctt
      961 gcgatttgtc tcttcctatt tagtaagagt cgtgcgaata aaacattagg taagctagct
     1021 attataccgt ctatttttaa tatcaataca gctattctat ttacgtttcc aacagtttta
     1081 aatccgatta tgctgattcc gtttattgct actcctacaa tcaatgcctt aattacctat
     1141 gtatcaatgg ctgtaggatt agtaccctac acaacaggtg taatccttcc gtggacaatg
     1201 ccaccgatca taggaggctt ccttgcgaca ggagctagtt ggcgaggagc tctattacaa
     1261 gttgttttga ttttggtttc tgtagcaatt tattatccat tcttcaaaat tgcagataaa
     1321 cgcaatcttg aaaaagaaaa agctactgtt ggagggaaat aagatggtta tcagagtatt
     1381 tgatcagcag aaaaatactt attctagctt tgccttagag gaattaagtt actatataaa
     1441 tcgggtcttt aagactgaca tagaacttgt cgaggagaag gaagcggata tttttgtagg
     1501 attagtcaat aaagaggata gaaaagacca tgttcttatc tcattagaca agggtaaggg
     1561 gagaattgag tctaatacaa ttgtaggttt acttattgga atttaccgaa tgtttcatga
     1621 atttggggtt gtgtatacta gaccagggcg cggacatgac tttgttccag agttacgatt
     1681 tgaagatttt ttagataaag agctatctat agatgaaaca gccagttact atcatagggg
     1741 agtatgtata gagggagcag attcatttga aaatatacta gatttcattg attggctacc
     1801 taagattggg atgaacagtt ttttcattca gtttgaaaat ccctattctt ttttgaaacg
     1861 ttggtatgaa catgagttta atccatacct aaataaagaa aaattctcaa atgaattagt
     1921 acaagaattg agtgataggt tggataaaga attgcaaaaa agaggtctta ttcatcatcg
     1981 tgttggtcat ggatggacag gtgaggtttt aggttactct tcaaaatttg gctgggaatc
     2041 aggtcttagt atttcagagg agaagaaacc ctatgtcgct gaaataaacg ggaaacgaga
     2101 attatttaat acggctccga ttttaaccag tctggatttt tcaaatccag atgtggcgga
     2161 taagatggta gaaattatca aggattatgc caagaaaaga cctgatgtta actacttaca
     2221 tgtatggttg tcggatgctc gtaataatat ttgtgaatgc gaaaactgta gacaagaatt
     2281 ggtttcggat cagtatattc gtattctcaa tcaattggat agggctttaa cgagtgaggg
     2341 attagataca aagatttgtt ttctgcttta ccatgagttg ttatgggcgc ctcagaaaga
     2401 aaaattagat aatcctgaac gctttaccat gatgtttgca ccgattacaa gaacatttga
     2461 aatgagttat gcagatgtag attttgacaa ttccatacct acgcctaaac cttatctgcg
     2521 taataaaatt atacttccga attctcttga ggaaaattta tcttatcttt ttgagtggca
     2581 aaaaacattt aaaggagata gtttcgtata tgactatcct ttagggcgtg ctcattatgg
     2641 cgatttaggc tatatgaaaa ttagtcaaac catttacaga gatgtatctt atctttctaa
     2701 cctacatttg aacggttaca tttcgtgtca agaattacgt gccggattcc ctcataattt
     2761 tcctaattat gttatggggg aaatgctctg gaagaagaca agaagttatg aagaattgat
     2821 tgaagaatac ttttctgctt tgtatgggtc aaattggcag tctgttgttg aatatttaga
     2881 aaaattatcc agttattcct catgtgatta ttttaatgca attggcagtc gtcaaaatga
     2941 tgttttagcg aatcattatt atatagctta caatctagct gataattttt tgccaattat
     3001 tgaggaaaat atttctaagt tattaaatag tcaaaaggat gaatggaaac agctcagtta
     3061 tcatcgtgaa tatgttgtta agatggcgaa ggctttatat cttcaagcaa ccggaaaaac
     3121 aaggcaagct caagatgaat ggagaaatgt gttgaattat atccgtgggc acgaattgct
     3181 atttcaatct aatttggatg tttatcgtgt aattgaagta gcaaaaaatt acgctggttt
     3241 ccacttataa atcataagta tagaaaatga accaaggtat tcagagaaga ttgatcctaa
     3301 atattatgaa atttaaggat ttttaagata tttagggtcg actttctatt tatatcgtag
     3361 cgaagtcatt ttaataatga tgtgtaaaag atggatcaag attgaggagg aagaaagatg
     3421 aaatcaaaag aagaaataaa tatgcttggt tttacaattg tcgcttacgc aggagatgca
     3481 aggtcagatt tgatggatgc tttggcgttt gcgagagatg gatattttga acaggcaaga
     3541 gaattggttg agtctgcaaa cgactcaata gtgtctgccc atcgagaaca gactaattta
     3601 ttagcggagg aggcatctgg agataatttt gaagtgagtt ttatcatgat tcatggtcaa
     3661 gataccttga tgacaacgat gctattgtat gatcaggtca agttttttat tgatgaatat
     3721 gaacgaattc gaaagattga agaacatatt ggtttgcaat gaggattagt catggaaaat
     3781 ttacaggtta aagccttacc gaaggagttt ttattaggag ctgctaccgc tgcttatcaa
     3841 gtagagggtg caactagggt agatggcaaa ggaacaaata tgtgggatgt ttatttgcaa
     3901 gaaaacagtc cgttcttacc agatccagct agtgattttt attatcgtta cgaagaggat
     3961 ataactttgg cggcagaaca tggtttgcag gctttgcgtt tatctatttc ttgggttcgt
     4021 atatttcctg atatagatgg ggagcctaat atattagctg ttcgttatta ccatagagtc
     4081 tttcagtctt gcttaaaaca taatgtgatt ccgtttgttt ctttacatca ttttgattcg
     4141 cctcagaaaa tgttagaaac aggggattgg ttgaatagag agaatattga tcgtttcata
     4201 cgatatgctc gcttttgttt ccaagaattt acagaagtca agcattggtt tacaatcaat
     4261 gaactgatgt ctcttgctgc aggtcaatat ataggaggtc agtttcctcc aaatcatcat
     4321 tttcaattat ctgaagcaat tcaagcgaat cataatatgt tgttggcgca tgctcttgca
     4381 gtcctcgaat ttcatcaatt aggaattgag ggagagatag gttgtattca tgctttaaag
     4441 ccaggctatc ctattgatgg gcaaaaagaa aatattttgg cagctaaacg gtatgatgtt
     4501 tataataata aatttctatt agatggaact tttttgggct actacagtga ggacacgctt
     4561 tttcacttga atcaaatatt ggaagctaat aattctagct ttattattga agatggtgat
     4621 ttagaaatta tgaagagagc tgcagctctt aatacgatgt ttgggatgaa ttattatcgt
     4681 tcagaattta ttcgtgaata caaaggtgaa aatagacaag aatttaattc aacaggaata
     4741 aaaggacagt cttcttttaa attaaatgct ctaggtgaat ttgtaaaaaa acctggtatt
     4801 ccgacaacag attgggattg gaatatttat cctcaagggt tatttgatat gttgcttcgt
     4861 atcaaagaag aatatcctca acatccggtc atttatttaa ctgaaaatgg tacagccctt
     4921 aaagaagtta agccagaggg cgagaatgat attattgatg acagtaagag aatccgttat
     4981 attgagcaac atttacacaa agttttagag gctcgagata gaggagtcaa tattcaaggc
     5041 tattttatat ggtctttgca agatcaattt tcttgggcga atggctacaa taagcgatat
     5101 ggtcttttct ttgttgatta tgaaacacag aagagatata ttaagaaaag tgctctttgg
     5161 gtaaaagggc taaaacggaa ttaaggttag cgatttgact gatgtttaat atattttaac
     5221 tatgagctta aattttttac aggaggagtt tttatggata agttagtcgc tgccattgaa
     5281 aagcaacaag ggaaatttga aaaaatttct actaataatt atatgatagc tattaaagat
     5341 ggatttattg ctactatgcc tttaattatg ttttcaagct ttttgatgat tattattatg
     5401 attcctaaaa atttcggagt agagttaccg agtccagcta ttgcctggat gaggaaagtg
     5461 tatatgctaa ccatgggagt tttgggtatt attgtttcag ggacagttgc aaagtcattg
     5521 gctggaaatg ttaatagaaa aatgcctcct ggaaaggtaa taaatgatac ttctgcaatg
     5581 ttggcggcca tatgtggcta tcttgtatta actgtaacgc ttgtagttga tgagaaaacg
     5641 ggatctacaa gtttgtcgac aaactattta ggatctcaag gattgataac ttcgtttgtc
     5701 agtgccttta ttactgtaaa tgtttaccga ttctgtatta agcgagacat tactattcat
     5761 ttacctaagg aagttcctgg ggctatatca caagctttta gagatatttt tcctttttct
     5821 tttgttttac ttattagtgg tttgttagat attgtatctc ggtttagttt agatgttcct
     5881 tttgcccaag tatttcaaca actattgact cctattttta agggggcaga atcatatcct
     5941 gctatgatgt tgatttggtt tatgtgtgct ttgctttggt ttgttggaat tcatggacca
     6001 tctattgtct tacctgctgt tacagcttta caactgagca atatggaaga gaatgctcaa
     6061 cttcttgcaa atgggcagtt cccttatcat tctttaacac ctaatttcgg gaattatatc
     6121 gctgctattg gaggaacggg ggctaccttt gttgtaccat ttattttgat tttctttatg
     6181 cggtctaaac aattaaaatc ggtaggtaaa gctacaatta ctcctgtttt atttgcggta
     6241 aatgaacctc ttctatttgg tatgcctgtt attttgaatc cctatctttt tgtccctttt
     6301 ttgatgactc caccagtgaa tgtatttcta ggaaaggtat ttattgattt ctttggaatg
     6361 aatggatttt atatccagtt accttgggcc tttcctggtc ccttgggatt gttaattgga
     6421 acgaattttc aacttatctc ctttgtattt ttatctttga tgttagttgt cgacatattg
     6481 atttatttgc cattctgtag agcgtatgat agacagttac tggtgaaaga agatattgca
     6541 agctcaaatg atattatttt agaggaggat acaagtgaaa taatgcctgg tgagatagat
     6601 gtaataaaaa gtaaggagtt gaaagtactg gttctttgtg cagggtctgg aacaagtgcg
     6661 caattagcca atgcaattaa cgagggcgct caattagcag aagttagagt gattgcgaat
     6721 tcaggagcgt acggagctca ttatgatatt atgggtgttt atgatttaat tattctggcc
     6781 ccacaagttc ggagttatta tagagagatg aaggtggatg cagaaagatt agatattcag
     6841 atagttgcta ccagaggaat ggaatatatt catttaacaa agagtccaag taaagcctta
     6901 caatttgtat tggagcatta ccaagctgtg tagtaagttt ttccatcttt tatttgagta
     6961 aagattttgc ttacagatag gcttggattt aaaaacgttc cccctttttt aatataagaa
     7021 tccctctttc acaattgtaa aaagagggat tttgtatttt atctcttaga ccaagttctc
     7081 ttcataaaga gaaggaggat tgggtaaatc tccaagcgcc ctgcaatcat tgcaaaggat
     7141 aggagaattt ttgagatggg actaaagatt gagaaactag aagtggttcc tagaataggc
     7201 ccgatattat tgaaacagct aaagacagcg ctggtcacga ccagaaaatc attgctatct
     7261 aggctgacaa taaagataag cgctagcaaa atcatagcat agatgacaaa gtacttgaga
     7321 atcttatgct gggtatcttt gtcaatcacc gttttattaa catggagggt caaaacacgg
     7381 tggggcgata ggattgacaa aatttggttt ttggcaattt ttgaaaggat gaggcctcga
     7441 ataatcttga gtccacctgc agttgatcca gcagagccac cgattgccat gaggaaaagg
     7501 aggataaact gggagaagag gggccagttg gtaatatctc catatccaaa accagttgtt
     7561 gtaatgatgt tggaaacctg gaagaaggtc atttcaaagc tctttgaaaa ccctgggtag
     7621 aggtagaggg tgttgaggct aatcaagcct gtagaaacca gtacaatgac caagtaagcc
     7681 ctaagctctt catctccaaa gaaggccttg atgcgacgga gcatgaggta gtagtagagg
     7741 ttgaaattta ctccaaaaac cagaactccg atactgacca gataggtaat cagtgagctg
     7801 ccatagtggg caattccgtc gttatagacg gtaaagcctc cagttcccgc tgtccccata
     7861 gcaataacaa aactatcgta gagaggcata ccggctagat aatagatgat gacaaagagg
     7921 gagaagagag ctagataaag gagatagaga atctgggcag tgttttttag tttggataca
     7981 accttgccaa aaacaggacc tggaacctca gccttcatca cctctaggtg gctatttttg
     8041 gcattgtcca taatagcaag tgcaaaaaca agcactccca tccctccaat caagtgggta
     8101 aaacttcgcc agaagaggag ggaacggctg agaaccgaaa cgtcgttcaa aatacttgct
     8161 ccagtagttg taaatccaga actaatttca aaaaaggcat caataaggct ggggatttgc
     8221 ccagaaaaga caaaggggag accaccaaag aaagaccaaa ggatccaaca gagggcaacg
     8281 atcaagactc cctccttggc ataaatccgt tgattttttg gcttctgtaa actccctgaa
     8341 ccgcctaaca atacgagaat ccctatggtc gaaaagaggg ctgtaaagac ttggctcgat
     8401 tcacggtaat agacagcaat cgcaacagga accaaaagaa gaacagcttc aatcaaaagt
     8461 aattttgaaa ggaggtaacg aatcatactt ttattcattt cttacctcgc gatcaagtca
     8521 taaatcttgg tgatgtttgg caacaaggtt gttactagga gcttgtctcc aacttccaac
     8581 atatcctccc cagttgggaa aatagtcttg ccctttcgaa taatggctgc aataagaacc
     8641 ccttttttta atttcagttg agaaagaggt ttggcagtca ttttattggc ttccttgata
     8701 tggaattgca gggtttcgat ttggccattg gctagatggt gcatggcttg aaggtctgaa
     8761 tactgggcat taactcgacc acgaataaag tgcataatcg tatctacagc gatgctttta
     8821 ggtgtgatga tacttgaaaa atcaggcgca ttgataatct cgaggagact ggtacgattg
     8881 accttagtaa tatttttctg tacacctacc ctgtcaagga acatagatgt aatcagattt
     8941 tcctcatcga cacctgttag agtcgcaacg gcatcatagt gttgagcact ttcttccagc
     9001 aggatatctt ttgcggttcc atctccttga acgatgtaga gatttgggaa tttctcgcta
     9061 aagaagctgg cgatttcagg attgatttca atgacttttg tatcgatacg actatctttg
     9121 agaataccaa gtagataata ggcaattcta cctgccccaa cgatgagaag gctcttcacg
     9181 gcgcgtgatt taaaataatt atggaagagt atcatatcga cacggttacc agtgacaaag
     9241 attctatctt tatcctgtac agtcatgtca ccgcttggaa tgataatttg atgatccctc
     9301 tctatcgcac agacaatgac attaccaaat tttttacgaa aatcagaaat gggcatttgg
     9361 caaagaccgc tggtggactt gacgacaaat tccatgaggc taacgcgtcc accagcaaag
     9421 cgttcgacag acagggcgtt ggggaagtca atgatattcg cgatagcgcg ggcagccaag
     9481 agctcaggat taacgataag agaaaaaccg agaatattct tttccttgaa ataagagtta
     9541 gaatattcag ggttccgcac ccgaacgata gtttctttag ctcccatttt cttggctaga
     9601 actgctgcaa tcatgttgac ttcatcgtgc tcagtcaggg cgataaagat atcacaatct
     9661 tggacgctgg cttgctcaag aatggcaaaa tcggccccgt taccaaggat accaatgata
     9721 tcaaagcgac tgacaatatg attgagaaca gcttcgtctt gctcaatcag caaaacatca
     9781 tgcttttctg caaccaagga gcgacagagg gcaaaaccaa cttttccccc tccgacaagg
     9841 ataattttca taataaaacc tactttttca tgatgtaact atcataccct ttttcaagaa
     9901 aaaatgcacc tactagctaa taaccagagt ttttagtagg aaatttgcta taagttaaaa
     9961 ctatacccta accaattgaa atagctatta gcgactttct ctgaaatatg gtatgataaa
    10021 ggatatacaa ggagataaaa tgaataataa tttactggta ttacaatcag actttggtct
    10081 ggttgatggt gcggtatcgg ctatgattgg agtggcttta gaagagtctc caaccttaaa
    10141 aatccatcac ttgacgcacg atatcacgcc ttataatatt tttgagggga gctatcgtct
    10201 ctttcagacg gtggattact ggcctgaggg aacgacgttt gtatcggttg tcgatccagg
    10261 tgtcggttcg aaacgtaaga gtgtagttgc caagactgca aaaaatcaat acattgtcac
    10321 gccagataat gggacgcttt cctttatcaa gaaacacgtt ggcattgtag ccattcgtga
    10381 gatttctgag gtggccaata ggcgtcaaaa cacagagcat tcttatacct tccacggtcg
    10441 tgatgtctat gcctatactg gtgctaaact ggccagtggt cacattactt ttgaggaagt
    10501 agggccagag ctcagtgtgg aacagattgt agagcttcca gtcgtagcga ccatcataga
    10561 agatcatctg gtgaagggag ccattgatat tctggatgtg cgtttcggtt cgctttggac
    10621 ctctatcaca cgggaagaat tttacaagtt ggaaccagaa tttggtgatc gttttgaagt
    10681 gaccatctat catgctgata tgctggtcta tcaaaatcag gttgtctatg gcaaatcatt
    10741 tgcagatgtg agaattgggc aacccattct ttacatcaac tctctctatc gtttaggttt
    10801 ggctatcaac caaggttcct ttgccaaggc ctataatgta ggtgtcggtt catcttggac
    10861 cattgaaata aagaaaatag aaggataaaa taggagaata gtatggaaat caaatttaca
    10921 attaaacaag ttgttgctgt cggaattggc gctgccctct ttgtcgtcat cgggatgatc
    10981 aacattccaa cccctgttcc aaatacaagc atccagcttc agtatgcggt acaagcgcta
    11041 ctttctatta tttttggacc gattatcggt ttgcttgttg ggttgattgg tcatgcaatc
    11101 aaggactctc ttgttggtta tggtctatgg tggacttgga ttatcgctag cggactcttt
    11161 ggtttagttg tgggactttt tagaaagtac gttcgcgtga tcaatggtgt ttttgactgg
    11221 aaagatattc ttatttttaa cctcattcaa ctacttgcaa atgcccttgt ttggggtgtc
    11281 ttggcaccac ttggagatgt tgtgatttat caagaagcgg cagaaaaagt atttgcacaa
    11341 ggaattgttg cgggaattgc caatggtgta tctgtagcta ttgcaggaac tcttctctta
    11401 cttgcctatg caggaactca aactcgtgca ggaagtttga aaaaggacta atactcaatg
    11461 aaaatcaaag agcaaactag gaagcaagtc gcaggttgct caaaacagtg ttttgaggtt
    11521 gtagatgaaa ctgacgaagt cagtaaccat acctacggca aggcgaagct gacgtggttt
    11581 gaagagattt tcgaagagta taaaatgatg gggaaggctg ggcaactagt ctttttcgat
    11641 gtttatagat tagttaggca agtaagctag gataggaatc tttttggcgc taagatttta
    11701 agagaagaga ctgcaagaag ggcttgattt atgataaaat agaagtctaa gaagcgaatg
    11761 aagagagtaa gatgaaagaa gctataattg agtggaagga tttctctttc cggtatgaaa
    11821 cacaacaaga accgaccttg caagggatag acttgaccat ttacaaggga gagaaagtct
    11881 taattgttgg accatctgga tcaggtaaat ctaccttggg tcagtgtttg aatgggatta
    11941 ttcccaatat ttacaagggt cagacatatg gagaattttt gataaagggt caaacagcct
    12001 ttgatatgag catctatgat aagtctcatc tggttagcac agttttgcag gatacagatg
    12061 ggcagtttat tggcttgtct gtggcagaag atttggcgtt tgctctggaa aatgatgtga
    12121 cagccctaga tgagatgaaa ggtcgtgttt ataaatgggc tgaaaagctg gaccttcttc
    12181 ctttactgga tcagcgccct caggatttgt caggtggaca aaagcagcga gtcagtctgg
    12241 ctggtgtctt gattgatgaa agtccgattc tcttgtttga tgagccactc gccaatctag
    12301 atcccaagtc aggtcaggat attatcgaat tgattgacca gattcataag gaagagggga
    12361 cgacgactct gattatcgag caccgtttgg aggacgttct gcatcgccct gtggatcgga
    12421 ttgtcttgat aaacgatggt cgtatccttt ttaatgggag ccctgaccag ttgcttgcga
    12481 ctgatttatt gactcaaaat ggaattcgag aaccccttta tctaacgact ctccgtcaat
    12541 taggtgtgga cttagtcaag gaagaacaat tagcagatct ggataacttg tctatctcaa
    12601 aagggcaagt ccagttacag acggaactgg taaaagaaac cccagaattg cagtcactct
    12661 ttagactaga ggaagtatct ttttcttatg atgatagacc gattttaaaa tccctacatt
    12721 tagatattaa aaagggtgaa aagattgcta ttgtcggaaa aaatggagca gggaaatcaa
    12781 ctctagccaa ggctataagt agctttattc agacggaagg acgctatctt tgggaaaaac
    12841 aggatataaa aggcgattct gttgcagagc gggcggaacg agtaggctat gtgctacaaa
    12901 atcctaatca aatgatttca accaatatga tttttgatga ggtggctcta gggctccgtt
    12961 tgcgaggtgt ggatgagaag gaaattgaaa cgagagtcta tgaaaccttg aaaatctgtg
    13021 gactttatga attccgtaat tggcctattt ctgccctgtc atttggtcag aaaaaacgtg
    13081 tcaccattgc ttcaattttg gtcttaggag ctgaaattat tctcctagat gaaccgactg
    13141 caggtcaaga tcagaagaac tatactgaga ttatggaatt tctcgaagag ttacatcaaa
    13201 aagggcatac cattgtcatg attacccatg atatgcaatt gatgctggat tattcagacc
    13261 gggtccttgt catggtggat ggagaattga ttgccgatac tgttccagcc agtctgttga
    13321 gcgatcctga gctgttagta aaagccaatc taaaagaaac ctccatcttt aacttggcta
    13381 agaaactaga tgtggatcca ctggatttaa cggcatttta caaagaaagg agagagggat
    13441 gcaagctaaa ttaatcggtt accagcatag agatactgtg attcatcgct tgtcaggagc
    13501 tgggaaactt ctctttttca ttctggtatc attggcggcc atgattagct atgataccag
    13561 aatgctcttg ctgattgcca tcttttcagt ctttctcctc tatttatcag aaattcgctt
    13621 taaagatgtt tcctttgtag ccgtttttgc gacggtattt gccgttttaa acgttttgat
    13681 ggtctatctc ttttctcccg agtatggggt tggactttat ggagagagaa gtgtgatttg
    13741 gcagggaatc ggtgtctaca ctctaaccag ccaggagctc ttttatctgc taaatttggc
    13801 cattaagtat ctttgcacca ttcctctggc tattatcttt ttgatgacaa cccatcctag
    13861 tcagtttgct tccagtttaa atcaaattgg tgtgccctat aagattgctt attctgtcag
    13921 cctgaccttg cgctatattc cagatttgca ggaagaattc tttactatca agatgtctca
    13981 ggaggcgcgt gggatggaat tatccaagaa agcttctctt atgcaacgaa tcaaaggcaa
    14041 tctgctcatt attacgccct tgatttttag ctcgctagaa cgcattgata ccattgcgac
    14101 tgccatggag cttcgccgct ttgggaaaga gaaaaaacgc acatggtata gttatcaggc
    14161 cttgaaaaaa ggagactata ttaccttgct cttggcaacc ttgtttttag tagttagttt
    14221 actacttatc ttgcagaatc agggacgatt ttacaaccct tggaaatagc ttgaaaaaat
    14281 tgaaaaaatc aagtcgtttc tattgacaat gattctgaaa gtgttatact aagaaagtag
    14341 tttcgctgat ttacttcaaa cctgttgtga ggtaagttaa cgatgcctta accacgctgt
    14401 ttgctgagct tgactccggg cagtgtggct atttttttgc aatggtgaaa ggaagcaagt
    14461 catgacaaat cacattgtat tatttgaacc tcaaattcca caaaatacgg gcaatatcgc
    14521 gcgtacttgc gctgcgacca attctcccct ccacatcatc aagcctatgg gctttcctat
    14581 tgatgaccgc aagatgaagc gggctggatt ggattactgg gataagctag agatttattt
    14641 ttacgagagt ttggaagatt tcatatctca gatgaaggga aaactctatc tgatttcgaa
    14701 attcgcggaa aaagtctatt ctgaggtgga tttatcgact gacgaggacc attattttct
    14761 ctttggacgt gaagacaagg gcttacctga agattttatg cgagaacatc ctgagaaggc
    14821 tctccgtatt cccatgaatg atgaacatgt ccgcagtctc aatgtgtcta ataccgtctg
    14881 catgattgtc tatgaggctc ttcgccagca gaactttgca ggtcttgagc tcgtccatac
    14941 ctatgaagtg gataaattga aataaaaaat gaaaatgaac aaaatgcttg cgcttgcaag
    15001 cgttttttgt tatgataaaa gagtcttcag ggcagggtgt gattcccgac cggcggtgac
    15061 tttaactagg aaatgatctt ttccttttat actttgttga caagctttgc ctaaccagaa
    15121 gttatgccta cagcttgtcg cctagtctaa aagaaaaatc tctatttcct tctctttaaa
    15181 gaagtccgcg agcgcaagct gatgtggtga gattccacaa ccgacagtat agtctggatg
    15241 ggagaagacg aaagaatagc tttgtctgtt ttgatagatt tatagccgga ttgtaaccac
    15301 ttgctttgtt tttctttaat agagtaagag ggaacttttg ggatataaaa agtgagaata
    15361 gatagaggaa tcctttccaa cttcttctga ttttatagaa aattggagga acctgttatg
    15421 acaaatacac gtcgactttc gaccattgca attttatcag ccatctcatt tgtgctgatg
    15481 tactttgact ttccgctttt accagcggca tctttcctca agatcgaatt tagtatcttg
    15541 ccagtccttg tgggtctggt ggtcatggat ttgcctgctg ctctaggagt gctcttgctt
    15601 cgctcactct tgaaattgct tcttaacagc caaggagtga atacttacat tggtttgccg
    15661 atgaatatcg tagctttggg agtttttgtc atcgtatttg ctttgatttg gaaaaaggaa
    15721 cggacaaccc ttcgtttcct actaggctct ctagctggaa ctgttggttt aaccttggcc
    15781 atgttggttc tcaactatgt ttatgctgtt cctttgtacg ctaagtttgc taactttgat
    15841 attggaaaaa ttttgggact ttccaactac ctaatgacca tggtattacc ttttaacttg
    15901 attgagggtg taatcttttc cgtttcattc tggttgttgt acgtcctttt gaaaccaacc
    15961 ttaaaacact atgaaagata aacaaacatt tttaatgaag ggcagttttg cccttttact
    16021 tttcgttatt cttggctata tggtcaaatt ttaccctgaa atgttggtca attttgacca
    16081 atcgattcag actgccattc gaggagactt accagattat ttgactattc tttttcgagc
    16141 cctcacacgt ctgattgata tcccagtgat tatcacttgg gttgtcatta cagcttttgt
    16201 cttttatcgt aagcgatgga agatagaaag tttcttcatg ctgggaaatc tggctttggc
    16261 aggtctttta atcgtgacct ttaaaaatat ctaccagcgc ccacgaccag ctatcttaca
    16321 tctggtggaa gagaagggat tttccttccc aagtgggcat tctctggctg taactttgat
    16381 ggtaggtact ctgattgtca ttctcagtca acgaattaaa aatccagtct ggagaaaaat
    16441 cgtacaaatc gtccttggcc tctacctagt cagtgtgctg gtatcaagag tctatctggg
    16501 agttcattac ccatcagacg tccttgccag tctctgtgtg ggcttgggag tcctgtttat
    16561 cgagtttccc ttctatgaca agctccgctt ccaatggcga tttaaaggca aacagaagtg
    16621 agtatcgaat tcccttgagg agaaagaaat gaaagtcaat atagcagatc ttcatctgac
    16681 tcaactatat ttatcagaaa agaagttgca agatattcag atgctttacc agtcggcaga
    16741 aacaatccaa gtcgatccaa tcagtattct tgcctttgga gattgtttgc tgattacaga
    16801 tgggcatcac agggcttatc aggctttatt ggcaggtcgg gatacgattt ctgctgagtg
    16861 ggatagagat ggtggtgatg aactatatca tctctatgcg caagcttgcg aggaaagaaa
    16921 gatttactct gtttttgatt tagaagatcg tatcctagct caagatgggt atgaagcaaa
    16981 atggtataac tggtgtgatg gttttaatca agcagcaact cttttgttga aaaggtagtg
    17041 cattgtgttt tgttccttat tataaggtca atcattgtga ggaagctttt gcttggtatc
    17101 aggatgtgaa cttggtttac ctcgtagatg gggtgaagct tccttacagt caagcgacct
    17161 tggaagccat gtattcctat ttggatcggc atggtgagct tttttggatt gaagtcaagg
    17221 agaagggaga atggtttcca attggggata ttacactatc tcaggataat ctccccattg
    17281 tgattgggaa ttccgcttac caacatcgag gacttggaaa aaagattcta agtgctttga
    17341 ttgaattggc tcgagtaaaa ggatggaaag aattgagagt caagaaaatc tacacctaca
    17401 atcatgcttc taggaggtgt ttcaagtcgc ttggatttgt ggaaaatgga gcaacagaaa
    17461 aaggaatgag ttttatattg gaattagtct aatcctgcca ctttctcccc cttaaccttt
    17521 gactattccg caaaattatc gtaaaataaa gagtgaatga taaaatgagg tcagagtctg
    17581 ttcgctctgg cgatagtagt ataaatgagg agaaacgctt tgaaattaga agtatttgct
    17641 gggcaagaaa aaagtgaact atctatgatt gaggtagcgc gtgctatctt ggaacttcgt
    17701 ggtcgcgatc atgagatgca ttttagcgat cttgtaaacg aaattcaaaa ctaccttgga
    17761 acatcaaaca gcgatatccg cgaagctttg cctttgttct acacagagtt gaactttgac
    17821 ggtagcttca tctcacttgg ggacaacaaa tggggtcttc gttcatggta tggtgtggac
    17881 gaaatcgacg aagaaatcat cgctcttgaa gaaaatgacg acgatgaagt aacaccaaaa
    17941 gctaagaaaa aacgtgtcaa tgcctttatg gatggtgatt cagatgccat tgactacaat
    18001 gcagatgatc cagaagacga agatgcatac gaagcagatc cagctctttc atacgatgat
    18061 gaaaatccag atgatgaaaa aaatgaagtg gaagcttatg atgcagaaat caacgaaatc
    18121 gctccagatg acttgggaga agatgtggat ctcaacgaag acgacgacga gttttcagac
    18181 gacgacgctg aaaccagcga ggaataagag ttagctattg acaattatcc tatttttagg
    18241 tatcatattg tttgggcacc tcttttagag gtcggggctc cctagttctt agggagctat
    18301 ttttgttttt tcaagaagtt atcttcttgt attttatact caatgaaaat caaagagcaa
    18361 gctaggaaac tagccgtagg ctgctcaaaa cactgttttg aggttgtaga taagactgac
    18421 aaagtcagga acacatatct acggcaaggc gacgttgacg cggtttgaag agattttcga
    18481 agagtattag ttgtgaatct ggtgcagtcg tcccagatta ttcttattag tagggtcttg
    18541 ttttctatat cccctcgtag ttaacaagac cttgagcatt ttagaaagag gaatctatgt
    18601 ctacgaaata tatttttgta actggtggtg tggtatcgtc cattgggaaa gggattgtgg
    18661 cagcgagtct aggccgtctc ttgaaaaatc gtggtctcaa agtaaccatt caaaagtttg
    18721 acccttatat caatattgat ccgggaacca tgagtcctta ccagcacggg gaagtttttg
    18781 tgacagatga cggagctgag acagatttgg acttgggtca ctatgaacgt ttcatcgata
    18841 tcaatctcaa caaatattcc aacgtgacaa ctgggaaaat ttacagtgaa gttcttcgta
    18901 aagaacgccg tggagaatac cttggggcaa ctgttcaagt cattcctcat atcacagatg
    18961 ctttgaaaga aaaaatcaag cgtgccgctc taacgaccga ctctgatgtc attatcacag
    19021 aggttggtgg aacagtagga gatatcgagt ccttgccatt cctagaggct cttcgtcaga
    19081 tgaaggcaga tgtgggtgcg gataatgtca tgtatatcca tacaaccttg cttccttacc
    19141 tcaaggctgc tggtgaaatg aaaaccaaac caacccaaca ctctgtcaaa gaattgcgtg
    19201 gcttgggaat ccaaccaaat atgttggtta ttcgtacaga agagccagct ggtcaaggaa
    19261 ttaaaaataa actggcccag ttctgtgatg tggcaccaga agccgttatc gaatcgttgg
    19321 atgttgaaca cctttaccaa attccactga acttgcaggc acaagggatg gaccaaattg
    19381 tttgtgatca tttgaaatta gacgcaccag cagcggatat gacagaatgg tcagccatgg
    19441 tggacaaggt catgaacctc aagaaacaag ttaagatttc ccttgttggt aagtatgtgg
    19501 agttgcaaga tgcctatatc tcagtggtcg aagccttgaa acactctggc tatgtcaatg
    19561 atgtagaagt caaaatcaat tgggtcaatg ccaatgatgt gacagcagag aatgtagcag
    19621 aactcttgtc tgatgcggac gggatcatcg taccaggtgg ttttggtcaa cgtggtacag
    19681 aagggaaaat ccaagccatc cgctatgcgc gtgaaaatga tgttccaatg ttgggagtct
    19741 gcttgggaat gcagttgaca tgtatcgagt ttgctcgtca cgttttaggt cttgaaggtg
    19801 ccaattctgc agagcttgca ccagaaacaa aataccctat cattgatatc atgcgtgatc
    19861 agattgatat tgaggatatg ggtggaaccc ttcgtttggg actttatccg tctaagttga
    19921 aacgtggctc taaggctgct gctgcttatc acaatcaaga agtggtgcaa cgccgtcacc
    19981 gtcaccgtta tgagtttaat aatgccttcc gtgagcagtt tgaggcagca ggttttgtct
    20041 tttcaggagt ttctccagac aatcgtttgg tagaaatcgt ggaaattcct gaaaataaat
    20101 tctttgtagc ttgtcagtat caccctgaac tgtcaagccg tccaaaccga ccagaagaac
    20161 tctacactgc ctttgttact gcagcggttg agaacagcaa ttagcaaaat cagaaccttt
    20221 gagaaaaatc tcagaggttt tttgcatacg atgatattgc agtatatctg aggtaggggt
    20281 cctctgtatg tacctgctac cgttgaaatc aatagcgact ccctcttgcc ctgtgctagt
    20341 gaatggattt atcagtatat tgaaatgaaa taaaatttga acaaattaat tcggaaagcc
    20401 aaatcaattt ctagcaaagt tttaggaact ggattgtata gtgaattgaa ataagatgtg
    20461 aacatctcta tcagaaaagt caaattaatt tatagaaata ttttagcagt caagatgtac
    20521 tgttatagat tcaatacatt atactttttt aatttaatcc a
//
LOCUS       DBERCA010000031        20360 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.031, whole genome shotgun sequence.
ACCESSION   DBERCA010000031 DBERCA010000000
VERSION     DBERCA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.031"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..159)
                     /locus_tag="ACL719_001271"
     CDS             complement(<1..159)
                     /locus_tag="ACL719_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HGS0699233.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYAD"
     gene            complement(318..1925)
                     /locus_tag="ACL719_001272"
     CDS             complement(318..1925)
                     /locus_tag="ACL719_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGS0699234.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENSNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2078..3562
                     /locus_tag="ACL719_001273"
     CDS             2078..3562
                     /locus_tag="ACL719_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGS0699235.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3657..4139
                     /locus_tag="ACL719_001274"
     CDS             3657..4139
                     /locus_tag="ACL719_001274"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGS0699236.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4435..6540)
                     /locus_tag="ACL719_001275"
     CDS             complement(4435..6540)
                     /locus_tag="ACL719_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0699237.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(7149..8129)
                     /gene="mraY"
                     /locus_tag="ACL719_001276"
     CDS             complement(7149..8129)
                     /gene="mraY"
                     /locus_tag="ACL719_001276"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGS0699238.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(8131..10383)
                     /gene="pbp2X"
                     /locus_tag="ACL719_001277"
                     /gene_synonym="pbpX"
     CDS             complement(8131..10383)
                     /gene="pbp2X"
                     /locus_tag="ACL719_001277"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGS0699239.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDSFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAVYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(10387..10704)
                     /gene="ftsL"
                     /locus_tag="ACL719_001278"
     CDS             complement(10387..10704)
                     /gene="ftsL"
                     /locus_tag="ACL719_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGS0699240.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(10716..11666)
                     /gene="rsmH"
                     /locus_tag="ACL719_001279"
     CDS             complement(10716..11666)
                     /gene="rsmH"
                     /locus_tag="ACL719_001279"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGS0699241.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            11832..12026
                     /locus_tag="ACL719_001280"
     CDS             11832..12026
                     /locus_tag="ACL719_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0699242.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            12042..12584
                     /locus_tag="ACL719_001281"
     CDS             12042..12584
                     /locus_tag="ACL719_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HGS0699243.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            12595..12837
                     /locus_tag="ACL719_001282"
     CDS             12595..12837
                     /locus_tag="ACL719_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699244.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            12848..13183
                     /locus_tag="ACL719_001283"
     CDS             12848..13183
                     /locus_tag="ACL719_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699245.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(13293..14294)
                     /locus_tag="ACL719_001284"
     CDS             complement(13293..14294)
                     /locus_tag="ACL719_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0699246.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(14354..16255)
                     /locus_tag="ACL719_001285"
     CDS             complement(14354..16255)
                     /locus_tag="ACL719_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGS0699247.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDMLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(16277..16570)
                     /gene="yajC"
                     /locus_tag="ACL719_001286"
     CDS             complement(16277..16570)
                     /gene="yajC"
                     /locus_tag="ACL719_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS0699248.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQTEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(16570..17388)
                     /locus_tag="ACL719_001287"
     CDS             complement(16570..17388)
                     /locus_tag="ACL719_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0699249.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVVIGIIGSWLKIIA"
     gene            complement(17375..18154)
                     /locus_tag="ACL719_001288"
     CDS             complement(17375..18154)
                     /locus_tag="ACL719_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS0699250.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            complement(18169..18660)
                     /locus_tag="ACL719_001289"
     CDS             complement(18169..18660)
                     /locus_tag="ACL719_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699251.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLACNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(18671..19861)
                     /locus_tag="ACL719_001290"
     CDS             complement(18671..19861)
                     /locus_tag="ACL719_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGS0699252.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(19873..20301)
                     /locus_tag="ACL719_001291"
     CDS             complement(19873..20301)
                     /locus_tag="ACL719_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HGS0699253.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFESGI"
BASE COUNT         5946 a         4623 c         3652 g         6139 t
ORIGIN      
        1 atcagcgtaa tagtctaata gatagttaaa tgtagttgga tttccactaa aggttgatga
       61 gtaagttttt gtatctgagc ctgattggcc gcaagcagct aaaagcaaag cagatgcaaa
      121 agtcaggcct gcactaagaa cacgcttctt tatgttcatc ttctttctcc ttaatagtgg
      181 gaatttgtaa agttaattga atttcgaaaa tgaaagttta taaaccttgg ttataaaaaa
      241 caaaggattt ctgcttttta tacagtcctc ccttgttttt atatgatttc aattttaaat
      301 ttttctgcaa aaaatattta tagtaattcc acacagaaag catcccatgg aactaagatt
      361 tgtttttcaa aaacttcttg agctagggtg ttttcaatca agacagattt gacttttcct
      421 tctactgtca agtcttgctc ttcattggac aagttagcca caactaggaa gcgacggtcg
      481 ccatccttac gtatataagc aaagacctta tcagccgtat caagcaattc aaagtcagct
      541 cgaattagcc agctattctc cttgcgaatt tggaccagtt tctgataggt atagaaaata
      601 gaatctggat ttgccagcgc ttcttggacg ttgatcatct cgtaatttgg attaactgcc
      661 aaccaaggtt gacctgttga gaaaccagcg tttttgctct cgtcccattg cataggggta
      721 cgggcattgt cacgtccaat aacacggata ctgtccatga tttcttgcat cggaacacct
      781 ttttcaagag cctcacgcgc atagttgaga gattcaatat cttctacttg atctagtgtt
      841 tcaaacggat agttggtcat cccaatctcc tcaccttggt agatataagg agttcctctc
      901 atgagatgaa gtaagattgc aaaggctttg gcagattttt cgcggtattc ttggtcattt
      961 ccccagattg agacaatacg agggaggtca tggttgttcc agaagaggga attccagccg
     1021 tcctcaactc ctaactctgt ctgccatttg ttgaagattt cttttaactt agcgatattc
     1081 agttcttttt gatagtgcca tttaggctga ccttcctgat actgaagacc gatatgttca
     1141 aactggaaga ccatagacaa ttcttgcccc tttggatcag agtagagctt ggcaatctct
     1201 ggcgttgctc cccaagtctc ccctactgtc aagagatcct tatctccaaa ggtcgcctga
     1261 ttcatttcct tgagataggg atggagcata ggaccattat tgactacctt ctcgtcagga
     1321 attttgccaa tcatgtcaat aacatccata cggaaaccac caataccttt atcaatccag
     1381 aagttcatca tctcataaat tttctggcga agtttttcat tttcccagtt gagatccggc
     1441 tgtttcttgc tgaaaaagtg gagatagtat tgaccagatt tttcatcgta ttcccaagca
     1501 gatccactaa aaatagaatc taaatcgtta ggctcatccc gccagatata gtaatctcgc
     1561 tcagggctat tagaattttc acaggcctct acaaaccagg catgctcatc cgaggtatga
     1621 ttgaccacca agtccatgat gatacgaatg tcacgcttct tagcttccgc aatcagttga
     1681 tccatatcct ccatggttcc aaaaatcgtt gcaatcgctt gataatcagc aatatcgtag
     1741 ccattatcat ccatagggct gtcataaacg ggagaaagcc aaatcgctgt gattcctaac
     1801 ttagctagat agtccaactt actggtaata cctggcaaat caccaactcc atctccatta
     1861 ctatccataa aactctttgg atagacttga tagactacgg cattatgcca ccatttttct
     1921 tgcatctgtt tacctcatta ttttcataat ctatagtata tgataacact ttttaaaatt
     1981 ttgtgcaagc gtttgcttaa tctttttaat ttctttttta gctccgtagt ttcctaactt
     2041 ccaatttttt attataatag aggtcagagg taaaaaaatg aaaaaacaag cttttagttc
     2101 tgaacaatat ttgaatttac aacgcgacca tattttggaa cgcattaacc aatttgacgg
     2161 caagctctac ttagagtttg gcggtaaaat gttagaagat ttccacgctg ctcgtgtcct
     2221 tcctggttat gaacctgaca acaaaatcaa gctcttgcaa gaattgaaag agcaggttga
     2281 ggttgtgatt gccattaatg ctagcaacat tgaacattcc aaagcacgtg gcgacttagg
     2341 catttcttat gaccaagaag ttcttcgttt gattgacaaa ttcaatgaat tagggatttt
     2401 tgttggttcc gttgtcatta cacaatacgc tggccaaccc gctgcagatg ccttccgcaa
     2461 tcaacttgag aaaaacggaa ttgattctta tcttcattat ccaatcaaag gatatccgac
     2521 ggatatggat cacatcattt ccccagaagg catgggcaaa aacgactaca tcaaaaccag
     2581 tcgcaacttg attgtcgtaa ccgctcctgg acccggttct ggaaaattgg caacgtgtat
     2641 gtccaatatg taccacgacc aaatcaatgg catcaaatct ggctacgcta aatttgaaac
     2701 cttccctgtt tggaatcttc cccttcatca tccagttaac ttggcttatg aagctgccac
     2761 agctgacctt gatgatgtca acatgattga ccccttccat cttcaaacct atggagaaac
     2821 cactgtcaac tacaaccgtg atatagaaat cttcccagtg ctcaagcgca tgttggaacg
     2881 tattctcgga aaatcaccat acgcttcacc gacagatatg ggtgtcaaca tggttggttt
     2941 cgctattaca gatgacgagg ctgctgtcga agcttctaaa caagaaatca tccgccgtta
     3001 ctatcaaaca gttcttgact tcaaagctga aaaagttggc gaagctgccg tcaagaaaat
     3061 tgagttactc atgaacgacc tcggtatcac acctgcagac cgtaaggttg ctgtcgttgc
     3121 gcgccaaaaa gcagaagaaa ctggtggacc agccctagcc tttgaattgc caaatgggga
     3181 aatcatcact ggtaagaact cagaactctt tggccctaca gccgctgcct tgatcaatgc
     3241 catcaaaaaa tcagctgaca tcgctaaaga agtaaaacta atcgagcctg aagttgttaa
     3301 gccaatccaa ggtcttaaaa tcgatcatct cggtagccgc aatccacgcc ttcattcaaa
     3361 tgaaatcctg attgcacttg ctatcacagc tacagaaaat cctgatgctg cccgcgctat
     3421 ggaagaactc ggcaacctca aaggaagcga agcccactca accatcatct tgactgatga
     3481 agacaagaat gtccttcgta aactgggtat caacgtaacc tttgacccat actaccaata
     3541 cgaccgctta tatcgtaagt aaagatttca acctctctag cctcggagag gttttctctc
     3601 tgtctcagga acaaccttta tgatataatg agagaagaaa actgaaagga tttaccatgt
     3661 caaaagaagt tattgtcgaa agttttgaac ttgaccacac cattgtcaaa gcaccctatg
     3721 ttcgcttgat tggggaagaa acaggaccaa aaggagacat catctccaat tatgatattc
     3781 gcttggtgca accaaacgaa gactctatcc ctactgccgg ccttcacact atcgagcacc
     3841 tcttagccaa actcatccgt acccgcattg acggcatgat tgactgttca ccatttggtt
     3901 gccgcacagg cttccacatg attatgtggg gacgtcacac cagtgctaaa atcgcagctg
     3961 ttatcaagga ttcgctcaag gaaatcgctg aaactactac ttgggaagat gtcccaggga
     4021 caaccatcga atcttgcgga aactacaagg accacagcct cttctctgct aaagaatggg
     4081 caaaactcat cttggaacaa gggatttcag atgatgcctt tgaacgtcat gtgatttaaa
     4141 atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtaccgt tcggcatacg
     4201 gcggttcaac taacttttaa cgcatgtcgt tcaaggtaat aatccaaaca cgaaaccagt
     4261 ccacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt ctgagctact
     4321 atagtatatt gaaactggaa taatacactt ctactatacg acatgcctgt cgtacctaaa
     4381 aaggaaccag tttttttcag ctggctcctt cttttttatc ctcaaaattc tatcttaggc
     4441 tttctcacga ataaccaaaa cgccatcttc catatctgct tccagatgtt tagcatctaa
     4501 atgatccaag tggaagtctg tcaccttatc acgaatttct tgttcaacca cgcgacggag
     4561 aggacgaacc cccatgactt cgtcgtaacc ttcttctgtg atataatctt tagccgcttg
     4621 actaactacc aagtcaatgt ctttcttagc caaggtttgg ttaacttcag ccaacatcaa
     4681 atctacaatc ttagaaaggt cttccttagt caagtgtgag aactcgatga ctgcattaaa
     4741 gcggttgagg aattctggac ggaagaaggg tttcaaacgg tccatcaatt ctggtttatc
     4801 cgcatcttct gtcaagttgg cttcatagcc aaatccagca tttgaggtcg caataacgac
     4861 agtattcttg aagtttactg tatttccttg accatctgtc aaacgaccat catctagaac
     4921 ttggaggaga agggtaataa cttgaggatc agccttttca atttcatcca agagaatgat
     4981 agagtatgga ttgcgacgaa cacgttctgt taaggtattg ctattgtcat cataacccac
     5041 atagcctgct gttgtaccaa ttagcttaga aacagctgtg cggtcactgt attcagacat
     5101 atccaaacgg ataatcgcat cctgggttcc aaacatatcg agtgccaatt gcttagcaag
     5161 ctccgtctta ccaaccccag tagaccctac aaagaggaag ctgccgattg ggcgatttcc
     5221 ttcatcaaaa ccagcacggt tacgacggat agcacgagct acaacttcta cggccttatc
     5281 ttgaccaatc accttttctt gcaagcgatg agccatatct ttcaaacgtt cgatatctga
     5341 agctcccatt tgcgatactg ggatacctgt cattcgttcc acagattcag ccacatcgtt
     5401 gacacttgca gtcactttca tatcttctgt gtggttttcg attttccttt ccaattctgc
     5461 aatgcgtgtt ttatagttta gagctgcttc aaaatcttct gcttcaactg ctttttcttg
     5521 cttgtctttt tccgtttcga tttctcgttc aacagcatgc acatctgtta ctggatgttg
     5581 agccgccaag tgagcagccg ttacatcgac aaggtcaata gccttatctg gcaagctacg
     5641 ttgaggaatg tattgaacag aataatccac cgctgctttc aagacttcgt ctggcaagat
     5701 gacattgtgg tgttgttgat agaggtcacg aatgccttga agaattttaa aagtattctc
     5761 tgctgaagga gcattgacct tcacttcgtt gaaacgacga gcaagagcag cattcttcaa
     5821 gatggtgtta cggtattcgt cttgagttgt tgccccaatc actgtcaatt ctccacgaga
     5881 gagagctggc ttgagaatat ccgcaagtcc tttagaacca ctgtctccac cagtgctacc
     5941 agcaccaaga atttggtgaa tttcatcaaa gaagaggata atattccctg cttctttcac
     6001 ttcattgact aagttttgga cattttcttc aaagctacca cggtattgag taccagcctc
     6061 aagacctgag atatcaatag aaataatttc cttgttcttg atagcagcag gaacatctcc
     6121 gttcacaatg gcttgcgcta gaccttcgac aactgctgtc ttaccaacac ctgcatctcc
     6181 gaccaaaaca ggattgttct tggtgcggcg tgagaggatt tcagatgttt cttgaatttc
     6241 cttgtttcgt ccgataacag gatccaactt gccctcacgc gcttctgctg ttaagtttcg
     6301 acctagtttt gcaaggacac cgtcttgttt catacctgat gcctgttgtg gcatttgcac
     6361 atcagtttct gcatttcctg gtaattgacc agtcgcacga tagtgagcaa attcctcagg
     6421 tgtgacttcg cgtccattaa ttaagtaacg gcgattttca gaactatatc ctcgcatacc
     6481 acccatcaat tggttaaata aatcatccat gttattaaaa ttattaaagt tgttattcat
     6541 attctttacc tctttttgtt tacttagtta tgattactga tattgactat ctttgaccat
     6601 tgttttaaaa aatttagact agctagatcg ctaccctacg tactatttct ggtttttctt
     6661 tttcaagcag gagtagacta tctttacctc tgaagattgc tagatgaaac atagactcac
     6721 ctctttctat tttactttaa ataagtgttc tagtaaggct ttcatttacc ttacgttcat
     6781 aatatactac attggtcaga aaaggtcaag agttttgact ttaactgacc aatatttttt
     6841 tacactcttc gaaaacctct tcaaaccaca tcagcttcgc cttgtctaca acctcaaaac
     6901 agtgttttga gctgacttcg tcagttctat caaccatctc aaaactgtat agtaaaatga
     6961 aataagaaca ggataaatcg ttcaggacag tcaaatcgat ttctaaaaat gttttagaag
     7021 tagaggtgta ctattctagt ttcaatctac tatattttga gcagcctaaa gttagctttc
     7081 tagtttgttc tttgattttc attgtgtatt taaacacaaa aacaccctga aacatcaggg
     7141 tgccattctt acatcaaata caaaattgca agggtcagga gacttgctag aagcccaact
     7201 ccccaaaaga agaagtcaac cttccactcg ctccaaggat ttcctttacc agacaatccc
     7261 ccaagctcaa aatggtgatg tacaggcgtc atacggaaaa tacgtttacc acctgtcagt
     7321 ttgaaataac tgacttgcat cataacagaa gttgtttcaa aaacatatac aattccgata
     7381 atcaagagag tccattcttg gtggagagcc atagagatag ctgccagcat cccacctagg
     7441 gccaaacttc ccacatcacc cataaagacc ttagcaggct tatggttaaa gatgaaaaaa
     7501 ctgagcaaac caccaatcat ggcaagaatc actagaagaa tatccatctg accttgcaca
     7561 taggcaataa ctccataggc agacaaacta atcacaacgg aaatactagc taaaccgtca
     7621 acaccgtctg tcaagtttac tgcgtttgaa aaaccgacta gccagaaaag agcgaagaca
     7681 atatagaaaa tccctagatg cacttgataa ccaaagacag aaagcatatc gccaccgcgc
     7741 tcatagaaaa gatagaagat aactccacct agaagctgaa gagctaattt ttgcttagga
     7801 ttaagcccct cattgatttt acgaaagacc ttgagaaagt catctaaaaa tccgaccaag
     7861 ccatacaaga ccaagatgaa caaaatcatt cccatattat tgctgaattg gctactaaat
     7921 agggcgaaaa agaaagcaac caaaacagaa gtaatcaaga aaaccaaacc tcccattgta
     7981 ggagtcccag cttttgcctg atgctgtttg acatcctcat gcatctgctg gcctgtaatt
     8041 tgcgcctttc tataaaattg gataaaggcc ggaattccta ctaaagttag taaaaatgtc
     8101 acaattccag cactgatgga aataaacata ttagtctcct aaagttaatg taattttttt
     8161 aatgtccttg atagctgtgt tagcacgaac atcttgcttc tgcacagtag agcccgaacc
     8221 ttgaaattca agttctatat tgagccactt agcaagggtc tcagcagtct cctttgtcca
     8281 accatacata tctggaactt cctctacttt atcagataag ataaggactt gctgattcgg
     8341 ggcaagattc ttcccttctt cagcagaact gtttttaatc ttcgttcctg ttcccacaac
     8401 gatgggttgt acaagattgc gacgcaattc ttctgctaaa tcaccaggtg aaatatcctt
     8461 gacactaggc ataggataag gactttgttg acttacttgc tccaaagctt tagctgttgt
     8521 ttgaagattg agagagtctt tcatagctga agcccgctcc aagataggat tggcaaattc
     8581 tcccaactga atacctgaat aatgttcagg ttgttggacc gtcacataca agataaaatc
     8641 aggattttca gccggactca tcgatacagc cgagaaaata tagtcggtta acccgactag
     8701 ataaccacca tttttctcgt cagcaatctg agccgtacca gacttgaggg ctacattttg
     8761 cccaggaaca gttacagttg gcttgcctgt gctgtggtta tacatggttc cataaaccgg
     8821 atccgtccct accaaaacca tgttagtccg agttagacta gctgcatctt tagaaacagg
     8881 atttcccaca atttcttttt gagatttccg agcagtttga tcatttggat cataaacggc
     8941 actaataaat ttaggctcca gcatgacacc gtcattagca atagctgtaa aggcacgaat
     9001 catttgcgtc tgggtcactg aaatcccttg tccaaatgag ctttgcgcaa tgttgacaat
     9061 attatccgca ggaagctgac cagcatactc atccgtcaaa ccgaaacggg tcggaactcc
     9121 aaacttaaaa cgattaagat aatcaagcca ggtagcatct cccatctttt gctcaaggag
     9181 ggtcatccca acgttacttg agtgtgcaaa accttgagaa aaagtcatca ttctgccacc
     9241 agtcaatcct tcattaacgt cccaatctcg aatcgtggca tctgcaattt ttaactcact
     9301 actattaaag acttctcctc ctggaaaggt attattatca atagcagcag ccaacatcat
     9361 cactttcata gtggaacctg gctcatagtt actttggtaa aggatatcac gccaaacaaa
     9421 gtcctctgta atgccttctt ttgtatctgc atcaaaggtc ggtcgttgcg ttgttgccag
     9481 aatttcccct gttttagcac tgaccaaagt cgctgtcatg tactttcctt ttaccttctc
     9541 ttgaaaagaa tccatctggg tttccataaa ggactggagg gggctggaaa tggttgtata
     9601 aacatcctta ccgtccatcg ttcgttggga aacttgttct gttccgggta caatattacc
     9661 cagacgatcc ttttcatagg taataatgcc gtctgtccct gcaagaatac tgttcaagga
     9721 actctccatt ccagaggttc ccagcaagct cttgcttcca tcttcatttt catggagctg
     9781 agctaggccg ataaaactag aagcaaattg tccgtttggg taactacgat tgggactggt
     9841 tgtaaaatca atccccttga cctctgcagc ttccaattct tttttgatag acatcatatt
     9901 ggcataggta atcccatttc cctttgctcc aaaggaaact tgcttgagat taggttgcga
     9961 gagttgctct cttacatagg attcttccat gtccagatac ttatgaaaga cctctgcaac
    10021 cttgttaaat tgtgtttttt ctacgtaaag aatcttaccc gttgctgact tatagttctc
    10081 atcaatgacc gcatagacat tataggaggt tgcatcctca gcaatcggga ctccatttcg
    10141 gtcataaata gtcccacgtt tggcaggaac tgtacgggtg gtttgatgaa ccttcttagc
    10201 ttccttcgct aaatctgttc caaagcgagt gcctgtccca ataatgaccg caaaattgac
    10261 taaaaaaatg gcaaaaacaa agacagataa taaactcaga ctttttccaa ctctgcgtct
    10321 gttttcagcc ggcgatttcc gatttttggt cgcataacgg attactcttt ttgtccactt
    10381 catatcttac tccgctattc taatattttc attgtttaat tgcaaatcgt gtgaattggc
    10441 aatttctttc aaacgttctg cacgtaatag ttcattgacc tcttgcttgg catcgtccaa
    10501 ttcggtcttc ttttcctcta tctgcgcatt gatttttgtc aaatcattct gcacttgcaa
    10561 gagcttggtc tgcataaaaa taatactaat ggctacaata agagtggtta cagcaatgga
    10621 aaagtaaaaa gctttttcca cacgcgaaaa ccgtttaagt tgcatctgta gtatttgacc
    10681 tgttttttcc attttttctg ccatcttttt ccctcttact tgtgaatttt tctggccacg
    10741 cgcaacttgg ctgagtgcga gcggttattg gcttctaact cttccgcact tggcaagatt
    10801 ggcttacggg acaccaattc catcttgggc ttgagatcat ctgggatgaa aggcaagcct
    10861 tttggaactt caactgttga agcttccttg aacaattgct tggtcaagcg gtcttctaag
    10921 gaatgaaagg taatcactga aattctacca tccagagcca acatatccat agcctgctgg
    10981 atggactcat ctgccgctcc cagttcatca ttgacttcaa ttcgaatagc ctggaaaatc
    11041 tgcttagcag gatgaccctt cttcttgagt tccttggcag gtttgaccaa cttgataatc
    11101 tctgctaact cagtcgttgt ctcaatcggc ttcacttcac gcgcttgctc aatcttacgc
    11161 gcaatctgtt tagagaattt atcctcaccg tatttgaaga aaatacgaac caaatcatga
    11221 tagtcatagt gattaaccac ttcataggct gtcagactag catcctgatt catccgcatg
    11281 tccagtggcg catccttttt ataagaaaaa ccacgctcac gctggtccaa ttgaggacta
    11341 gacactccca agtcataaca aattccatca atttcctgaa caccagcttc gcgcaaacgt
    11401 gcctgtaaat gacggaagtt atccttgata aaggtcacca ttcccttctc aatgtaaggt
    11461 gccaagcgtt tttgcgcatt gtcaatggca ttctgatcct ggtcaaaggc atagagatgg
    11521 cctttttcac ttaatttact taataaatac tcgctatggc ctgctccacc caaagtcgca
    11581 tcaacgtaga taccgtcagg ttttacgtca agcatatcaa tcgtttcgtg gagtaagacc
    11641 gttacatgat gaaattcttt tgtcatatct tatctatttt accacaaatc cgaccagctt
    11701 gcacttgtca gacaaatagg acaaggagac tactatttct tcaaaaaata tgtcaaatat
    11761 acttgacact ttatgtttaa aagactataa tatagtcaaa tatatacgac ataaatcaga
    11821 aaggagacca gatgaatcgt gtgaaagaat ttcgcaagga attgggtatt tcccagctcg
    11881 aactcgccaa ggatatcggt gtctcgagac agactatcaa tatgattgaa aacgacaagt
    11941 acaatccaac cttggaactc tgtctcaatc tcgcccgcag cctccaaact gacctcaaca
    12001 gtctcttttg ggaggaagat ttttaaaaaa ggagcaaact catgaaaaaa gaaaccttca
    12061 ctgaaaaact gatcaaacgc acatacggta tttctggtcc ccttgacgaa tacaaacggc
    12121 gtgaggccga tagtattggg aaccaagtct ttatcgtcct cttttatctg atgattttcg
    12181 gaaatcttat tccactcctt ctagcctata aataccctca agaagtggct ctaatctatc
    12241 ctcctctgat tttagtgatt gccctcatcg ctgctggcta tgtcacctac caaatgaaaa
    12301 aaacaggcat cacagtcatt gaaccagata tgctgaatga gaaagaaagc aagcaactac
    12361 actacccagg tctgaaagca ggtttgttct ttggtctatg gatgtttttt ataactcctc
    12421 ttctcagtat actcatagat gaaggtcagg actattttca ttctcttctc actataagaa
    12481 atggtgtatc aagtattctc ggttctattt tcttcggagc gagcatacag ttcctcatct
    12541 cccgtcgcat tgcaaaaact aagaaaaatc aagatgagga ttaggaggaa actcatgaaa
    12601 aaagaaaaga aacagttacg ttatcctggt cttaaagcag gttcgattta tggaacagtg
    12661 atatttttta taattccact cattgatacc ctaacaagtg aaaatccaaa ttttatcagt
    12721 tctcttctaa atacaaaaca tatttttaaa actatactgg gagctttctt tttcggagtg
    12781 atgatacata ttgtcgactc acttcgtatt gcaagagcta aaaaagacca ggattaggag
    12841 gtgccttatg aaatcactag ctagactact gaacattcat gtttttatca gtattttcct
    12901 tttcttcgcc cttatttcag gagctgtttc tcatacagtt ttactactcc tactcctctt
    12961 tcttcctgcg ctcaataaag gacttgagaa aatacaatca aaacggatac ctgtcctcaa
    13021 cgcagccctc ttctttctcc tcatatcctt tccacaactt ttaaccaacc ctgtccaatg
    13081 gaaattttca atattcctag tcgtaaccat catttcaagt ttggcctact tctataactt
    13141 ttatcaagta gttaaagaag tagatcaaaa acagttgatt taggagcttg ctcccataat
    13201 ttctcatttc ataagcaaat aatcccacct agcttgttct aggtgggatt attttatttc
    13261 ttaacagaga ttgcaagtca ttttccttca ttttagaaag tcgactcttt ccaattcaca
    13321 ctacaatcca agacttgttg cctttcttct tgattacggc cttcaatctg atcaatcaaa
    13381 atctttgtag cctgttgtcc ttcctcaaag gagggctgaa ccagcgtcga aacacttgga
    13441 gaagaaaagc aagtccactc cgtattgtca aaaccaatca acccaacttg tggcaagtta
    13501 taattcaact ctttgataac ggtaaagact agaggtaggg cccaacagtt agggataaat
    13561 accagagttt tttcatcggg atcgatttct ttttgtaaaa attccttaat ttgttccaaa
    13621 ttcgtatgct tatcttcaat ggttagactg gcgtgacgca tattagcatc tgttaaagca
    13681 tccacaaaac cacttgcccg ctcaatccga gtactcaaac gactcgtatc cgctgtaatc
    13741 aagagaaaat gctcataacc tttttcgata caggactggg tcatgtcata aacggcatca
    13801 tagttattgg ttttaaccca gctagtccgg tgttcataga gctgactatc aaaaaagacc
    13861 attttcttct ttttctcatc gatgatacga gaatattttc ggaaattaga ggtcggctga
    13921 ataataaagc cgtctactcc caagagaagc atgctttcaa tataccggtc ctcactctct
    13981 tggctgtaat tactatttcc tatcattacc tggtagccat tctggctggc gatatcctca
    14041 attcccttaa caatttggtt tgagaaactg ttggtaatat caccgatcaa aacaccgatt
    14101 aattttgttc gtttggagtt taagctacgc gcaacaatgc tcggtttgta atttgtttca
    14161 tgaataactt tttcaatctt ttcacgtgtc tcttgggaca ttttttcata tttcccgttt
    14221 aggtaaaatg acacggttgt tttcgaggtc tgagccattt ccgcaatgtc ttttatggtc
    14281 agtttcttct ccaatctaaa acacctccta ctaatactta ttataacata ttcactattc
    14341 aaaatgccta tttctagcac tgcaagcgaa tcattctctc atttatttta tcatatacta
    14401 gacattttcc acgcattttt gttccgtcaa cgaggcacaa tttttcacct acacaaatat
    14461 cctcgcttaa taacagaatc agcttgtctg tttcgtcatt tttcacttca aaagccagag
    14521 cattttccac ttcacgatca tctgtctggt agacagaatg acggatgatt tcaaaactct
    14581 catgcgcaat gatggtataa tccagcatct gattctcaaa gaattggcgc ttggtgagtt
    14641 tgttacttct ttcaagctca ttgtatttag gagaaatgat ggtatcttcc aaatcaaaat
    14701 caacttcact ccatagtctc aactgattga ttttcccatc ttgataggtc acatccttgt
    14761 caaggataaa ctgagtcaac gcctcatgct gaccttgaca cctgatgtca tccaccaaga
    14821 gccagacatc ctctaccaac atgaggattt ttctcttgtg aagataaggc aaatcaggtt
    14881 ctgctgacca ataagcccct tcaatataat gcactccctc cctttcttta tggtgacaaa
    14941 acagggagtg aggatagtat tcatattccc aggatcccgt gattctttcc ggagctttcc
    15001 catctacaat gcaggtcgaa tgactccaag cactctttaa gagataacgt tcatatatct
    15061 cccgataaga ataacgccca gcatctatga aaataggttg tccttgatac tgtaagcaaa
    15121 aactattctc gtcactatgg ctatgggcac ttcctagcgg accatttttg aaaaatagat
    15181 aacgatgttc atccttaatg cagacatgtc cagagtcttc aaagatcatg gacttaggct
    15241 gccaagctct cttttcaaat tcctgcagtc gcttgacctt ttctcgcccc aggaacaaga
    15301 ggctaagcaa atcgacttta acatccagac cgttaagaag gtcttccttg ttcaaaacca
    15361 cagcagacag gctcaaaatt tctgtcgttt ctgtagaatc gctatcacca aaagccaaag
    15421 ttcgtccatc taagcctgtc atcatttgaa tataggtcgc catcttttcc agcaactctc
    15481 ggtaactatc ttgcaagtct ggaagcaaga gacacaaatc cagcaaggct ttataaacct
    15541 ctacatgata gagaatcgac tgttcaaact ggcttccatc tcctaaaatc tgtgtctcaa
    15601 tttgctgttt caactcctct gaagcaaaat ggtaagcttc ttctaggtcc atcttatctg
    15661 aaaagaaata atagatagca agcatcggaa ttgtttgtaa aatcccccag ttactaaggg
    15721 tgtacttggc gcgatagtag cttttcataa agtcaatctg cttttctaga ctgaccaaaa
    15781 ttttctctag ttctttctcc tctagcatgt caaatttcaa gaggagcaag agtagtttca
    15841 accaagtaaa ggaacgaata cccgtatcca aggttctagt catcaaggat tgaggagaaa
    15901 attctctcac ctgctcaatc caatcaaata gaaagaactt gcacttttga atatagtcct
    15961 tatctccttc taccacatac cctatcataa actgcaagag atattcttgt cgattgagca
    16021 tataagccca ttctggatca tcttcaaata cttgatccca taccatcggc tggatttgat
    16081 ggatttttga acaaggctcc atatcccaag gactatcaaa cataaaacga ttgtccatca
    16141 agcgttcaag ggaactcttg actttctcat agtcttttga acagtgcgac aagatataat
    16201 cacgacattg gtttccatcg actctttcaa aaaattgtct tctttcttct ttcattatct
    16261 attaccagaa aaagaactac ttaaaaagca gttcttttgt ctttcccatt acactttcct
    16321 tttctacata gatgaccaca ccttttgcaa tctgcaagga gaccaagtca tctttgatag
    16381 aaatgatttt tccatgaatt ccagacaata acaacacttc atcaccagat gttaaagaag
    16441 ctaaatactc ttgtcgttgc tccgtctgtt tgcgaagcaa cttttgctga cgaatagaat
    16501 gaaagcttga cagtaaaagg ggactcactg ccaagacaat cactattcca taaaacaatg
    16561 ttgtatccat taagctataa tcttaagcca gcttccgata attccgatga caactgttaa
    16621 aataacgagt ttatatgttg tccatttctt ttctttgatc aagtagtaaa ctaaaagtgt
    16681 aaatagggct ggtagaagag ctggagcaac cttatcaagc attccctgaa tacttacgat
    16741 actttgttta gcgtctgctt taacttcccc tgcagcaaag gtaatcggca ccataatctt
    16801 aacagatgtc gctgccaaac cagcaattac ggttacaccg ataatattgg caatacgaga
    16861 aatcgttgcc atctgttcgc ttagtttatc aatcacagtt gttcctagtt tgtatccata
    16921 cagaccagtt gacaatttaa tcgctgttaa aatcgtattc atcgcaagga agaacaagat
    16981 tggaccgaca accaagcctt cttgagcaaa cgaagctgcg atggttgaga acaatggagc
    17041 taaacagaat tgagaaagag aatccccaat acctgccaat ggtcccatca aggccatctt
    17101 gatgctacgt gtttcttttg ccggacggcc attttccaac attacaagat gcaagctggt
    17161 aataaaaggc aggaagtgtg ggttggtatt atagaattca cagttttctt ccaaggcttg
    17221 gtagaaacct tcctgatcct ctccatagtg ttttttcaaa gcaggataca tcacattggc
    17281 atatcccaac ccttgatagt tactatagtt aaatccattt tgacaaaaga atgcccgcaa
    17341 agacgtttta agataatcac gttttgttaa tttgttagat ccagtcatcg tgtgcttcct
    17401 cctctaccac atgattcgct gtttttggct tgttataaaa ttcaatcaaa gcaaagatag
    17461 tacctacaat tgcaatacca attgttggga tgtttagata agctgcacaa acatatccca
    17521 acaagacaaa gggaatcaac tctttcttag ccatcactga caagatcatc gcaaaaccga
    17581 tagctgggag cattttacca gcaactgtca aacctgtaag taataccggt ggaatgtagt
    17641 ctacgagttt caacaaggta tccattgaaa gggcacccaa gcaacccaag gtaaatccaa
    17701 taaaggcaaa caaccaaatt gttgcattta gagtaaactt gaatttcttc aaattatggt
    17761 ttttcaagtg cttcatcgcc gtttcaggcg caccagcacg tacagtgtag gcgaaagttt
    17821 gtaagaattg aatcgccaca gcaatcggag tagagagggc aagagccgct tctggactga
    17881 ctttaccagc actagtgata gccatcaagg taccaaagat accaggtccg attgggtttg
    17941 gtggaacagt acctccagca ccaacaccga atcccatata agccaattca ccaatagctc
    18001 ccattgcaag agcagtaggc agatcaccta gaataattcc gacaccaaat gacagaacaa
    18061 tacatctatt ggtgtaaatt cctaacagca ttccactaaa acagaaagct gtccataatc
    18121 caattaaaat cgcttgaaat acattaatcg acataacgat ctcctctttt aaatatagtc
    18181 cataatgttg acttgaacag ctccatcatt tcctgttgga gttgttttcg tattaaatgt
    18241 tacttgatga gtttggctta attccttgag ggctgccttg tcctcttcac ccaggaagat
    18301 ggagcgtgtc acttgctctt taccaggggc attgtgaatg ttcccaatat tgatttcttt
    18361 gatagggaca ccaccttcta ccaaggttaa agcgtccttt acatccttta caacgataaa
    18421 gatcgtttga gcaggattag ccttgtgaat gatatcaatc accttttgca aagggaagaa
    18481 acgcatggca actgagtctg gcacaactgt tttcatcaga gtttgttgca tcttgtccgt
    18541 gcttacttcg tcattggcaa caatgaccgt attacaagct aggtatttta cccaaagttg
    18601 tccttgtcca tgaatcaacc gttcatcgat acgggtcata ataatatttg gcattgtcat
    18661 atttctcctc ctaccaatat aggttccagt ctttgtagaa acggataagg gcttctaggt
    18721 aatagtagtc accccagata ttgccttcat ccactccttt acctgaatgc catgagtaca
    18781 caccgtggag aagacttgtc ccaccagggg taaattgatc atttgcataa tgttcgatca
    18841 aggaacgaag catggcatgc atagcatgtt tataaatatc tttgtcagca tccacctctg
    18901 ggagatgttt tagcatttca tgaatcccac agacggcgat agctgttgct gaagaatctc
    18961 gtgattgagc actaccatca ttaaaaatca aatcccaata ggacacatga tcttttggca
    19021 gacgattcaa gaaataattg gtcacaccct taaacaagtc aaagcaggac tcgtctttta
    19081 agtgacgata agtcaaagga ataccataga ctccccatga ttgaccacgt gcccagcatg
    19141 aatcatcact atacccttgt ctcgttacac ctttaaaggg ttgacctgtc tcaggatcaa
    19201 aatagaaggt gtggaaggac gaagcgtcat cacggattac attattagct gaagcataga
    19261 aatgactttc tgcaatatcg tagtattttt gatcgcctgt ttcttgataa gcaaagaata
    19321 agagttggat attgagcaag cagtcgataa tcaaacggta atgctctttc ttgcccaagt
    19381 ctccccaagc ttgaataaaa ccaccttttt cttgatagcg ttcaatcaac ttatctgcag
    19441 ctttcaaggt tgcttctcta gcctctccat ctccatttat cttatattca gccatacaag
    19501 acggtgtgta caagaagccg agatcatggt gatccaattc tactctctta ttgacacgat
    19561 ccaggaaaga aagaacattt ttatgagcga tgtttttaaa tgcatcctgt tgactgtatt
    19621 cataagccaa ccacagttct cctgtccaga aaccattggt ccattccgtg ttatccatga
    19681 ttggatagac attatcaaag gtagctggcg tcgggaaatc ttccttgaaa tagtcaaggt
    19741 tgagttctaa ctgccgaata accttatcga ttgcctggcc gacttcttct ttcgtcagaa
    19801 gtggtacttc taagaagcgc tcaggcgatt ttattttttc aatcgtaacc ttttttatca
    19861 ttcttttacc ctttaaatac ccgattcgaa atcttcttcc tctgcatccg ttgaaaacaa
    19921 ttctttacca tttacgactc cgccctgggc cgccactact gatttattaa caacctcatc
    19981 aaagctatga gccattctag caaagactgc ttccattaac atggctaagt tcaaacccga
    20041 gagaacattc attgttttga ctggattttc tcccattatg gtagaagaaa ccttgaatgg
    20101 cgatcctccc aagagatcac ttaggattaa aacttcttct tcatttgaaa ttgcaagtaa
    20161 gattttttgc ttgagttcat ctgctgacat tccttccaca aagtcaatcg cctccacatt
    20221 ttcttgatta cctgcaatca attgtaaaga actataaatc cctgtagcaa aatgtccatg
    20281 ccctacaagt acaattttca tcttttctcc tttcttgttt aggtttaccg gtttacttta
    20341 tattccccat tgtaatcctt
//
LOCUS       DBERCA010000032        20280 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.032, whole genome shotgun sequence.
ACCESSION   DBERCA010000032 DBERCA010000000
VERSION     DBERCA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.032"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            117..3218
                     /locus_tag="ACL719_001292"
     CDS             117..3218
                     /locus_tag="ACL719_001292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGS0699254.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVLEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3301..4308
                     /gene="pfkA"
                     /locus_tag="ACL719_001293"
     CDS             3301..4308
                     /gene="pfkA"
                     /locus_tag="ACL719_001293"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGS0699255.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLYSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEADSKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4367..5872
                     /gene="pyk"
                     /locus_tag="ACL719_001294"
     CDS             4367..5872
                     /gene="pyk"
                     /locus_tag="ACL719_001294"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGS0699256.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6050..6397)
                     /locus_tag="ACL719_001295"
     CDS             complement(6050..6397)
                     /locus_tag="ACL719_001295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0699257.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDKYITIAKTFVEIHLNFLINLC
                     ISYFILNENQRAN"
     gene            complement(6729..6836)
                     /locus_tag="ACL719_001296"
                     /pseudo
     CDS             complement(6729..6836)
                     /locus_tag="ACL719_001296"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
     gene            complement(6946..7200)
                     /locus_tag="ACL719_001297"
     CDS             complement(6946..7200)
                     /locus_tag="ACL719_001297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGS0699258.1"
                     /translation="MGTKIGISKNTIGNYEKRVRSTKKNTIFDLVKVFSSLIDALFSP
                     VQKDSPSDIQSIYDQRAPSRQGKVLTYAERQLYDQKNEVS"
     gene            7636..8508
                     /locus_tag="ACL719_001298"
     CDS             7636..8508
                     /locus_tag="ACL719_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGS0699259.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPTDDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8641..8998)
                     /locus_tag="ACL719_001299"
                     /pseudo
     CDS             complement(8641..8998)
                     /locus_tag="ACL719_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9141..9452
                     /locus_tag="ACL719_001300"
     CDS             9141..9452
                     /locus_tag="ACL719_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699260.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKECEELRQIITTIFEQKMNKHSI
                     K"
     gene            9456..10172
                     /locus_tag="ACL719_001301"
     CDS             9456..10172
                     /locus_tag="ACL719_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0699261.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEEFICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10301..11116)
                     /locus_tag="ACL719_001302"
     CDS             complement(10301..11116)
                     /locus_tag="ACL719_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGS0699262.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQVTNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11113..12018)
                     /locus_tag="ACL719_001303"
     CDS             complement(11113..12018)
                     /locus_tag="ACL719_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGS0699263.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(12019..12150)
                     /locus_tag="ACL719_001304"
     CDS             complement(12019..12150)
                     /locus_tag="ACL719_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGS0699264.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKAKAEKKN"
     gene            12430..14559
                     /locus_tag="ACL719_001305"
     CDS             12430..14559
                     /locus_tag="ACL719_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGS0699265.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGKKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14546..14995
                     /locus_tag="ACL719_001306"
     CDS             14546..14995
                     /locus_tag="ACL719_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGS0699266.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            15054..15326
                     /locus_tag="ACL719_001307"
     CDS             15054..15326
                     /locus_tag="ACL719_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS0699267.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGRFW"
     gene            complement(15680..15871)
                     /locus_tag="ACL719_001308"
     CDS             complement(15680..15871)
                     /locus_tag="ACL719_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699268.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16299..17057
                     /locus_tag="ACL719_001309"
     CDS             16299..17057
                     /locus_tag="ACL719_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699269.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17059..19047
                     /locus_tag="ACL719_001310"
     CDS             17059..19047
                     /locus_tag="ACL719_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699270.1"
                     /translation="MFRLTNKLAVLNLIKNRKLYYPFALAVLLAVTLTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVIITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDNGYKIKEKEVFRYTYFGVANQEGNKLTFFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19089..19763)
                     /locus_tag="ACL719_001311"
     CDS             complement(19089..19763)
                     /locus_tag="ACL719_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGS0699271.1"
                     /translation="MDSNFAGRLNILRAGVLGANDGIISIAGVVIGVASATTNIWIIF
                     LSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GECKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLL
                     EQLFSI"
     gene            complement(19705..>20280)
                     /locus_tag="ACL719_001312"
     CDS             complement(19705..>20280)
                     /locus_tag="ACL719_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS0699272.1"
                     /translation="GQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSR
                     HQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYA
                     SWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMAN
                     LNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
BASE COUNT         6199 a         3706 c         4288 g         6087 t
ORIGIN      
        1 caaaaataca aatggataga aaataaatta gtaagctgat gaaatttttc tcaagagaag
       61 ccatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag tgtaaattga
      121 tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt tcgatcgaaa
      181 agtatgtgag agcagctaaa gaatacggct acactcattt ggctatgatg gatattgaca
      241 atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt catcctttgc
      301 tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc tttttagctc
      361 tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg cagggggaga
      421 aaacttggtc agtcctgtcc cagtacctgg aggatatcgc ggtcattgtg ccttattttg
      481 atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat ccagaaacac
      541 tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct tttgaaagca
      601 gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg ctcagagaag
      661 ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag aaactattcc
      721 aagagcgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc atttcttacg
      781 acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca gtagaggagt
      841 tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa gaatatcaag
      901 atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat tatttcttgg
      961 ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga atgggaaggg
     1021 gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt gacccagtag
     1081 agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg cctgatattg
     1141 atattgatat cccagatatt tatcgtccag attttatcag atatgttggt aataaatatg
     1201 gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag caagctcttc
     1261 gagatgtctt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt actaagaaaa
     1321 tcagttttcg tgacaatctt aagtcggcct atgagggcaa tctccagttt cgtcagcaaa
     1381 tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata gagggctatc
     1441 caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat ttaaccaact
     1501 acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct catggagttg
     1561 aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc tttgtccaga
     1621 agatgcaaga gttgcttgct gaaacagaag gtattcatct gaaaattgaa gaaatcgatt
     1681 tagaagacaa agaaacgtta gctttatttg cctctggtaa tacaaaaggt atctttcaat
     1741 ttgagcaacc aggtgccatt cgtctgctta agcgtgtgca accagtctgt tttgaagatg
     1801 tcgtcgcgac tacttctcta aatcgaccgg gtgctagtga ctatatcaat aattttgtgg
     1861 caagaaagca tgggcaggaa gaagtgactg ttctggatcc agtactggag gatattttgg
     1921 ctccaaccta cggcataatg ctctatcagg agcaggttat gcaggttgcc cagcgatttg
     1981 ctggatttag tcttgggaag gccgatattt tgcgtcgagc tatgggtaaa aaggatgctt
     2041 ctgctatgca tgagatgagg gcttccttta ttcaaggctc catagaagca ggccatactg
     2101 cggaaaaagc agagcaggtt tttgatgttc tggagaagtt tgcaggctat ggttttaaca
     2161 ggtcacacgc ctacgcctac tcagccttgg ccttccagtt ggcttatttt aaaacacatt
     2221 atccagccat attttatcag attatgttga attctgctaa cagtgattat gtaacagatg
     2281 ccttagaagc aggttttgaa gtagccccat tgtccatcaa cacgattccc tatcacgata
     2341 aaattgccaa caaggccatc tatctaggtt tgaaatccat taaaggagtc agtaatgatt
     2401 tagctctctg gattattgaa catagacctt attctaacat tgaagatttt atagctaaat
     2461 tacctgagaa ttatctgaaa cttcctctgc tagaaccttt ggtaaaagtt ggtcttttcg
     2521 attcatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta tttgaatttg
     2581 tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa tcggaagatt
     2641 ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt gtcagcaaac
     2701 atccactaca agctattgca agtaaggcta tttacccgat taccccaatc ggaaatttgt
     2761 cagaaaatag ctatgctatt attttggttg aagttcagaa aataaaagtg attcgtacca
     2821 aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa ttggatgtca
     2881 ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga gccttctact
     2941 atgtaaaagg aaaaatacaa tcacgtgatg gccgtctgca aatgattgca caagaaataa
     3001 gagaagcagt tgctgaacgc ttttggatac aggtgaaaaa tcatgaatcg gatcaagaaa
     3061 tttcacgtat tttagaacaa tttaaaggcc caatcccagt catcatccgg tatgaagagg
     3121 aacagaaaac catcgtttct ccccatcatt ttgtagctaa atccaatgaa ttagaggaga
     3181 aattgaatga aatcgttatg aaaacgattt atcgctaaaa atacggaaaa tagaagaatt
     3241 ttaaaatcaa atgtgatata atcaagaaga atgttaaaag aaaaaggagc ataaaccaat
     3301 atgaaacgta ttgctgtttt gactagtggc ggagacgcac ctggtatgaa cgctgctatc
     3361 cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tgtttggtat ctatgacgga
     3421 tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt aggagacatc
     3481 atttctcgtg gtggtacttt tctttactca gctcgttatc cagagttcgc tcaacttgaa
     3541 gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt agttgttatc
     3601 ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt cccagctatt
     3661 ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat cggttttgac
     3721 acagcggtta ctactgccat ggacgctatt gataagattc gtgatacatc atcaagtcac
     3781 cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc tctttgggct
     3841 ggtattgcaa ctggtgctga tgaaatcatc atccctgaag cagactccaa gatggaagat
     3901 atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat catcgtctta
     3961 gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc tggagataca
     4021 agcgaccttc gtgtaacaga acttggacat attcaacgtg gtggttctcc aactgcgcgt
     4081 gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa agaaggtatc
     4141 ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat tcttggtact
     4201 gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt taacaaccca
     4261 cacaaagccg atattgagct atctagcttg aataagagct tgtcataatt tacaatttag
     4321 ttaataagac caaaaaggtc atatatataa aggagtcaca aaaatcatga acaaacgtgt
     4381 aaaaatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa aattcggtga
     4441 ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca ttgctaaatt
     4501 gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc accaagaaca
     4561 aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa aagttggttt
     4621 ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg aagctaaaga
     4681 atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa tcaaatcaac
     4741 tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg atgttgaagt
     4801 tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg ctaaagatga
     4861 tgaaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta aacaaaaagg
     4921 tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg ataacgacga
     4981 tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg tacgtactgc
     5041 aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac atgttcaatt
     5101 gttcgctaaa atcgaaaacc aacaaggtat cgataactta gacgaaatca tcgaagcagc
     5161 tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg aaatggttcc
     5221 agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg ttatcactgc
     5281 aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag aagtatcaga
     5341 cgtattcaac gctgttatcg acggaactga cgctacaatg ctgtcaggcg agtctgcaaa
     5401 cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga acgctcaagc
     5461 tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact ctaagacaga
     5521 agtaatggct tctgctgtta aagatgctac tagctcaatg gatatcaaat tggttgtaac
     5581 tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa atgctgacat
     5641 cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact ggggtgttat
     5701 cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg ctgaacgtaa
     5761 agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg ctggtgtgcc
     5821 agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt aagaaaaata
     5881 taaaaaccta tcatatccag ctttctagtc tgtatgatgg gctctttttt tgtccagggg
     5941 ggcaaaaagg gggcaaaact ttcataatgg attgatactc ttcgaaaatc aaattcaaac
     6001 cacgtcagcg tcgccttacc gtactcaagt acagcttgcg gctagcttcc tagtttgctc
     6061 tttgattttc attgagtatg aaataagata tgcacaaatt gattaggaaa ttcaaatgaa
     6121 tttctacaaa tgttttagca atcgtaatgt acttgtctag attcgatctg atatattttc
     6181 gatttaatga tatggtattt aaaacctcca aagtagcttg ctccattctt ttacttacgt
     6241 gagtgtagat gttatttact gttttagcgt ttttgtgttc cactctaacc attatagcat
     6301 tcttctcagc tagtgtacta aggagtgtgt gcctgaaaat atgggaacta aggggctggt
     6361 ttatcggttt ctctagttta gtatttgcct tttgcaaagt gatgttaaat gcctttctct
     6421 aaatttacat atcactattg tttaacaaaa tctaatctac tttaggtcac ttattctttt
     6481 tttgaaatgt agaatgaact ttttcaaagt ttttcgaatc ttttaaaatc tgtttgtttt
     6541 atatcgccat tctccccctt ttttaattct ccctatatag cctgacaact ttcccgatgg
     6601 tacgaatatg gttgctttcg tctaggtgga tgtcggggta tttgggattg agttttttta
     6661 aggtagcctt ggcggagttt cttgacatag ttagcgccgt ctacttggaa gatgccgatg
     6721 gtattatagt caatctgtgg ggtattcttg ataaataggt agtcgctgtt tcttatcttt
     6781 ggctccatgg acttgctgat gacataagcg attgggtcgt agtcgtctgg gataatggaa
     6841 actccatatc taaatcgttg tcctgcatcg agcggctacc tgcagagata aactatctaa
     6901 cacgagagta agtagtctgt ctgtagtcgt ccagtctgat gatttttacg atacttcgtt
     6961 tttctgatca tacagttgcc tctcggcata ggtcagaact ttaccttgtc tggatggtgc
     7021 ccgttggtcg tagatagatt ggatatcgct aggagaatcc ttttgaactg gagaaaagag
     7081 ggcatcaatc aagctactga atactttaac taagtcaaat atagtatttt tcttagtaga
     7141 cctaaccctt ttttcataat ttccaatggt gtttttactt ataccaatct tagtacccaa
     7201 ttcttattga gttcaaccat tactagtcta tattgtttta tagttgattg agtttggaat
     7261 agtacgctgt agctgctaaa acatttctag gaattaattt gcctttccta atagagttgt
     7321 tcatatctta tttcaatcta ctatattttt cgcctctaac aatcgcaatc tcttctttat
     7381 ctacgaacga aatcgctttc tatttttgta agtaaagcat cacacgaaat ccacgaaaat
     7441 gaaaaccttt gttgtgtttt cgtaaaaaat ttgttgacag agcacgaaac gcctactata
     7501 attaaatcaa gcttaaggaa ataacaaaaa aacaaatcgg agggaaagac gatgaacaca
     7561 ttaaatgaga aagtaatcaa tatctgtaaa gcaatagtta aagaaacttt aatccaagac
     7621 atttaggaga aagaaatgaa gaaatcaaga aaattagcca cattaggaat ctgttcagct
     7681 ttgtttttag gcttggctgc ttgtcaacaa caacatgcta cttctgaggg gacgaatcaa
     7741 aggcaaagca gttcagcgaa agttccatgg aaagcttcat acaccaacct aaacaaccag
     7801 gtaagtacag aagaggtcaa atctctctta tcagctcact tggatccaaa tagtgttgat
     7861 gcatttttta atctcgttaa tgactataat actattgtcg gctcaactgg cttatcagga
     7921 gatttcactt cctttactca caccgaatac gatgttgaga aaatcagtca tctctggaat
     7981 caaaagaagg gcgatttcgt tgggaccaac tgccgtatca atagttattg tcttttgaaa
     8041 aattcagtca ccattccaaa gcttgaaaag aatgaccagt tgcttttcct agataatgat
     8101 gcgattgata aaggaaaggt ctttgattca caagataagg aagagtttga tattctattt
     8161 tcgagagttc caactgaggc aactacagat gtcaaggttc acgctgaaaa gatggaaaca
     8221 ttcttctcac aatttcaatt caatgaaaaa gctcgaatgc tgtctgtagt cttgcacgac
     8281 aatctggatg gcgagtatct gtttgtaggc cacgttgggg tcttagtacc tactgatgac
     8341 ggtttcttat ttgtagagaa attgactttc gaagagccct accaagcgat taaatttgct
     8401 agtaaggaag attgctacaa gtatttaggc accaagtatg cggattatac aggcgaggga
     8461 ctggctaagc cttttatcat ggataatgat aagtgggtta aactttaata gtgttatgat
     8521 atgacagtaa tcttaggatt tttatttcta tttttgatga gttggtgggt tatttcaatc
     8581 gttaatagcc agaaaccacg caaaaatgaa acatttattg gctatatcca aagatacgat
     8641 attgatggga atggctatca atcattaccg tgtcctcaga actgagagga gttcttgaaa
     8701 tcgtaaaact actttgaaca agagttactt caacccattg gatccgacgg agtaagttgc
     8761 tttcgtggat accaaaaaca gccgcaattt cttcataagt gcggtattct cgcacatatt
     8821 gaagagtggc cataagaagg tcttctaggc ttaatttagg ttttcgtccc ccttttgtgt
     8881 gtttaagttg ataagctgtt tttaatacag ctaatatctc ttcaaaagtg gtgcgctgaa
     8941 caccaacaag acatttaaat cgtgcatcag ttagttgttt acttgcttca taattcatag
     9001 aactactata ccatattttg tttcgcagga agtctaatgc taaaatctta tcagtgagaa
     9061 gatttcttaa agtttattct ttgtaatttc atctgtatta gtacataatt taacttagag
     9121 aaaataggag gactaatcga atggaattaa aagattttac agaaaaagaa caggaaatga
     9181 ttaagaaaag gcttacaatg tctaatatta gtgacaaaga aactactgag aagattctgg
     9241 cgctagtacc acaagacttg attaagcgaa tcccgttttt tgtcagaaaa catgctacaa
     9301 cacgtacgat taaacgcatt tcaattgaac accctgaact ctacgctgta gctcaaacaa
     9361 gtggtgagat tccagaaaaa gaatgcgaag aattgcgtca gattatcaca actatctttg
     9421 aacaaaagat gaataagcat agtattaagt agagaatgaa aaaatatttt attggcggtt
     9481 tgggaagcaa tgcctatcat agcaaggatt ttcttcaaga actagattcg caggtctatt
     9541 ttctaaatcc atatgaaaag catcttcgag atgaaacaga attgaaatca tggtttaaaa
     9601 atgagattgt agaggaagaa tttatctgtc tgataggtca ttctcttgga ggagatttag
     9661 ctcgttatct cgcatcggaa tttgaagaag taaagaaact gattcttttg gatggtggct
     9721 atctagattt agataagatt ttacctttgg atacagagtt agaggaaact aaaaattata
     9781 tcaaatctca aattgttttg gacttagatg ttcttacttc taaagaaaaa tctgaagcaa
     9841 agcattggtc agaaaatatg gagaaagctg taagacagtc ctatcactgg aatgttgagt
     9901 ataatagata tgagttggct ataaattatg aaaatataga agcgatactc cgcctacgga
     9961 gaaaaataca agcttttaag agagaagtgg gagatacctt gtttattagt cctcgctatc
    10021 ctaatgaagc tacatggaga gaggaagccc taaaagaatt gccagactat tttgatacta
    10081 tttttttaga aaactttggc catgagcttt atactcaagc acctaaagaa atcgctagtc
    10141 taatgaatga gtggctcgct tattttctat gagctacttg tattgctgga aatggtataa
    10201 gatgaaaatc tacaagtcac taattttcat ataacaaaaa acgaacaggc tctttttctg
    10261 ttattcagaa gagaacttgt tcgtttttat cttgattttc ttaaaaagct ctatatacat
    10321 agggattttc tggtttatcg actttggtaa agctgtcgat ttccaaggtt ggaaggtttt
    10381 gtttgagttc tttatggtag tgattgacca gttttccttt ggctgttatc caagtgttgt
    10441 tttcatactg ccgggtattg cccttggtca gcaatccata gacaccagaa tctgcgatac
    10501 agtggataat gccgaatcgg aacagaaatt gactattggc atgactgggg tcgttataga
    10561 caaagcctgt aaactggatt gtcttgccct caaactcatc tggatagtcg tagatagcct
    10621 ccatgacttc catatagttt tcattagtga cctgaatact atcttgggat aagtatttat
    10681 ccgccgccgt tcgcatttcc ttttcatagg ctgattttga aaaataagaa ctggtatctg
    10741 gtttcaaata ttggcttgtc gtcccttcgc ttgtctgaat ggctagatcc gttccttccg
    10801 atagggggaa atgataacct ttagcagaaa cagtctgaga atccaagcta acagttggga
    10861 aagttaagcc gatgacaagt ggaatagcca gaagagaaat actcgtcatc ttggctaatc
    10921 ggctgttcag atgactgtgg gttttgactt gcttcatcca gatatacaat tgaacgatag
    10981 ccaagataaa agaaagcacc atggagatat aggccagata ggaatagtgc atgttgatgt
    11041 actggtttag tttgcccgac agatggaggt aaatagtcag ttcaaaatag ccagctaaaa
    11101 ctaaaaatcg aatcatagga tgactccaat caagagagaa taggctaaaa cgaccagagt
    11161 cacaatcgta atgaagtgac taataaatcg tgctttcaag taatttttca tcataagaat
    11221 atttttgata tccagcattg ggccaatgac gagaaaagcc agaactggag ccaaaccgaa
    11281 actcgagagt agagaagccc cgataaaggc atccgcctca ctacagagcg aaagaagaaa
    11341 ggccaaaatc atcaagagca ggatggcaaa aagaggagtc gcactgatag aggtcagaat
    11401 ccgagtcgga acataaacct gtataacaga ggcaaagaga cagccaaata ccaaataacg
    11461 tcccgtatca aaaaactcat caatggcttg cacaaagacc tgaaaaactt tttttgcagg
    11521 acttaagtga gaaaaatcat gctcatgaca ggctagacga ttttcttttt gaatcggttc
    11581 ttgccagaaa aatcctagaa aaattcctag tattacagcc acaacgatgg aacccagagc
    11641 tcgtaatagg gcgacatgga aggagttgcc aaaggcagaa taggtcgcaa aaagaacaat
    11701 gggattgata acaggtgctg tcacaagaaa aggaacggcc gtgtaacttg gaaccttttt
    11761 ttccagaaaa cgattgatga tggggacgat tccacattca caggaaggga aaagtatacc
    11821 gacaaaggtc ccaaaaaaga ttctccccca acgatttcga gggagaaaat gataaacctt
    11881 gtcaggtgtg atataaactt caatcagccc tgagacaata cttcctatca gaacgaaggg
    11941 aagggcttca atgataatgg agagaaaaat ggctccagtt tgaagtacac tagatgggag
    12001 ttgttgaaaa gcagtcatct agtttttctt ttctgctttg gcttttttct ctttgtcatc
    12061 agggaaagtg acttgtttca aatcagggag tttagcaaac tcttcaaaca tcttgtccaa
    12121 gtcgtctcgt tttgtaaatt tgacagccat aggtgtacct cgattctaaa ttctacctct
    12181 attatactat tattttgcgg aaaagggagt tttttaattt gttttcgata gttgatactc
    12241 ttcgaaaatc tcttcaaacc acgtcagctt cgccttaccg tagtacggtt actgacttcg
    12301 tcagttctat ccacaacctc aaaacagtat tttgagcagc ctacggctag cttcctagtt
    12361 tgctttttga tttttattga gtatgaaaag gattggagaa agccttccag cttgtggtat
    12421 aatagattta tggataaaaa atatgaaaaa atctctcagg atttgggagt gacgttaaag
    12481 caaattgata ccgttctaag tttgacagct gaaggggcga ctattccctt tatcgcgcgt
    12541 tatcgcaagg acatgactgg tagtctggat gaggtggcga ttaaggctat tattgatttg
    12601 gataaaagtc tgacaaatct caatgaccgt aaggaagctg tcttagctaa gattcaagaa
    12661 caaggtaagt tgaccaagga attggaagaa gctatcttag ttgccgaaaa attagcagac
    12721 gttgaagaac tctatcttcc ttataaggaa aagcgtcgta ccaaggcaac cattgcccgt
    12781 gaagctggac tctttcctct tgctcgcttg attttgcaga atatagttga cttagagaaa
    12841 gaagctgaaa agttcgtctg tgaaggattt gcgactggca aggaagcctt gaccggtgca
    12901 gttgatattt tggtcgaagc cttatcggaa gatgtgacct tgcgttctat gacttatcag
    12961 gaagtgctga gacactctaa actcacttct caagccaagg atgaaagtct tgatgaaaag
    13021 caggtttttc agatttatta tgatttttca gagacagttg gaactatgca aggctatcgt
    13081 accttggctc tcaatcgtgg ggagaaactt ggtgtcttga agatcggttt tgaacatgcg
    13141 acggaccgta ttcttgcctt ctttgctact cgtttcaagg tgaaaaatgc ttatattgat
    13201 gaagttgttc agcaatccgt taagaaaaag gtcttgcctg ctattgagcg tcgtattcgg
    13261 acagaattaa ctgagaaagc tgaagaggga gctatccaac ttttttctga caatctgcgc
    13321 aatctcctct tggttgctcc actgaaaggg cgcgtggttc ttgggtttga cccagccttt
    13381 cgtacaggtg ccaagctagc tgtcgtggat gcaacaggaa aaatgctgac aactcaggtt
    13441 atttatcctg ttaaaccagc atcagctcgt caaatcgaag aagccaagaa agatttagca
    13501 gatttaattg gtcaatacgg tgtagagatt attgccattg gaaatggaac ggccagtcgt
    13561 gaaagtgaag cttttgtagc ggaagttctg aaagatttcc ctgaagtcag ctatgttatc
    13621 gttaatgaaa gtggtgcttc tgtctattct gccagcgaac ttgctcgtca ggagtttcca
    13681 gacttgaccg ttgaaaaacg ctctgccatt tctatcgccc gtcgtttgca agatcctctt
    13741 gcggaattgg tcaaaatcga tcctaagtca attggtgtcg gtcaatacca acacgatgtc
    13801 agtcagaaga aactctctga gagtctggac tttgttgtcg atacagtggt taaccaagtt
    13861 ggtgtcaatg tcaatacagc tagcccagct cttctttcac acgtagctgg actcaataaa
    13921 accatttctg aaaatatcgt caaataccgt gaggaagagg gaaaaatcac ttcacgctcc
    13981 caaatcaaga aagttcctcg tttgggggcc aaggcttttg aacaggctgc tggtttcctc
    14041 cgtatccctg aaagtagcaa tatccttgat aatacaggag ttcatccaga aaattacgct
    14101 gccgttaagg aactcttcaa atgcttggac attaaggatc tgaatgaaga ggcccaaagc
    14161 aaactcaagt cgctttcagt caaggaaatg gcgcaagagt tagaccttgg tccagaaact
    14221 cttaaagata tcattgcaga tcttctcaaa ccaggtcgag atttccgtga ttcctttgat
    14281 gctccagttc tccgtcaaga tgtcctagat atcaaggact tagtggttgg aaaaaagcta
    14341 gaaggagtgg tgcgtaacgt tgttgatttc ggtgcttttg ttgatatcgg aattcatgag
    14401 gacggtctca tccatatttc ccatatgagt cgcaagttta tcaaacaccc tagtcaagtg
    14461 gtggcagttg gagatttggt gagcgtttgg gttaagaaaa ttgatactga acgtgaaaaa
    14521 gttaatctgt ctctcctagc tccaaatgaa actgactgat tacgtcaagc aggtttcact
    14581 agaagacttc ggcagacctt ttatccatca tgtccagtgg aataggcgtc tacgttcgac
    14641 aggtgggcga tttttcccca aagatgggca tttggatttt aatcccaagg tttatcagga
    14701 actggggttg gacgttttta ggaaaattgt ccgacatgaa ctctgtcatt atcaccttta
    14761 tttccaaggg aagggctatc aacacaagga tcgggatttt aaggaacttt tgaaagcagt
    14821 ggatggatta cggtttgtac catccttgcc caatagtaac tctaaaccac tcaagcttta
    14881 tcgttgtcaa tcctgccagc aaaggtatca gcgcaagcgt aggattgata ccaaacgcta
    14941 tcgctgtgga ctttgtcgag gtaaattgct tctgataaat cagcctgagg actgatgaaa
    15001 gccgagcctc cccgtgatat actatgtcta acctagcaga aagaggaaat atcatgaaca
    15061 gtaaatttta taaaatgaga cgcaatcgca tgatttcagg agttctggca ggtctgtcag
    15121 acaagtggaa ttttgatgtt accctagttc gtttcttgtt tgccattttt acagttgcaa
    15181 actttggact tggcgtgatt atctatatca tcctagcttc tatcatgcct accaaggaag
    15241 aaatcgaagc agaaatgtac ggaacaggcc cacgtaaacg caaagaagcc caagccattg
    15301 acgacaatga aggcaggttt tggtgaggtt ctacagaaga tttggaataa gcaaaaagct
    15361 ttaaatcaat agtctaaact gcttgattta aagctttttt acttttatac tcaataaaaa
    15421 tcaaagtgca aactaggaag ccagccacag gctgtaattt agtcggcaat gtgaagttga
    15481 catagtttgc actttgattt ttgaatagta ttaataatat ttcgccagct tcatccacaa
    15541 actatagtgg attggaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg
    15601 atttgactat cctgatcgat ttgtcctgtt cttatttcat tttattataa aagcagtaat
    15661 ttaaactcag ttttttaatc tatttttgct gctgagacaa tacatttttg gaaaaatggt
    15721 ggatgctgat aacaactgat aaccagcaaa taacataagc agtgccggta aattctgtac
    15781 cgataaagtg tgtgtgttca actaagagct gaccgatgaa tggagagagc agatagagaa
    15841 gtccgatgag tatatgttct ttgtgtttca ttaaagcgca ccttctttcc ttataggttg
    15901 aacgacttgc aagtgttaaa ttgaatggta tgttatctag gtaaccttac taaaatgtta
    15961 gttcccgctt cctccttttc aaagtaagcc ttttcacact tatattttac aactttaaaa
    16021 ataggaaatc aaatcatttt ctaagtaatt ttgcttatat ttcacccaat cttacaaaaa
    16081 tgtaagatta aagtctcttt tgtaaggtta ggttatggca agcggtcttt ttttgatgta
    16141 aactagactc ataaagaaca aaggagtaac atcatgaaac aaatcttaca taagaaaact
    16201 agaaaaccaa gtcacaaaga aattgagcgc gttcagctgg gatgcactat gatgcaagct
    16261 acatttcatt tgatgggata ttaagaaagg agattctcat gacactttta gatgtaaaac
    16321 acgttcaaaa aatttataaa acacgttttc agggcaacca agtagaagcc ctcaaggata
    16381 ttcactttac cgtcgaaaag ggtgactacg ttgccatcat gggtgagtct ggttctggta
    16441 aatcaactct tctcaatatt ctagctatgc tggataaacc aagtcgtggt caggtttact
    16501 tgaatggaac tgacaccgca accattaaaa attcacaggc ctctagcttc cgtcgtgaaa
    16561 agttgggctt tgtcttccaa gactttaact tgctagatac tctatctgta aaggacaata
    16621 tcttgcttcc gcttgtcttg tcaagaagac ctattacaga gatgatgaag aaattggtgg
    16681 tgacagctga gaatctggga ataaaccaat tacaagagaa gtacccttac gagatttctg
    16741 gtggccagaa acagcgtgta gcagtagcac gcgccatcat cactgaacct gaaattctcc
    16801 ttgcggacga accgacagga gctcttgact ccaagtcatc tgcagcctta cttgatgtct
    16861 ttgatgaaat caatgagcgc ggccaaacca ttctcatggt aacccactca acagcagcag
    16921 ctagcagagc taaacgtgta ctctttatca aagacggcat tctttacaat caaatctacc
    16981 gtggagagaa gacagaacgt cagatgttcc aagaaatctc tgataccttg actgtcatgg
    17041 caagcgaggt gaattagtat gtttcgatta accaataagt tagcggtatt gaacttgatt
    17101 aaaaaccgca aactctacta tccttttgcg ctggctgttc tcttggcagt cactctcacc
    17161 tatctctttt actctctaac cttcaatcct aagattgcgg aaatccgtgg aggaacaacc
    17221 attcaggcta cacttggatt tggtatgttt gtcgtcaccc ttgcgtcagc cattatcgtt
    17281 ctctatgcca atagttttgt catgaagaac cgttccaagg aactaggaat ttatggcatg
    17341 ttgggcttgg agaagcgtca tcttatcagt atgaccttta aggagttagt ggtatttggg
    17401 attctaactg ttggagcggg tatcggtatt ggagccttgt ttgacaagtt aattttcgct
    17461 ttcctgctca aactaatgaa attgaaggtt gagctggttg ctaccttcca gacgaaagtt
    17521 atcattacag tgcttgttgt cttcggtttg attttcctag gcctcatgtt cctgaatgcc
    17581 cttcgaatcg cccgtatgaa tgccctccag ctctctcgtg agaaagctag tggagagaaa
    17641 aaaggtcgct tccttcctct ccaaaccatt cttggttcca taagtttagg aattggctat
    17701 tatcttgccc ttacggtaaa agatcctctt acagccttaa caaccttctt catagctgtt
    17761 ttactggtta tctttgggac ttatctcttg tttaatgcag ggattaccgt tttcctccaa
    17821 atcttaaaga aaaataagaa atactattac caaccaaata acctcatatc tgtttctaac
    17881 ttgattttcc gtatgaagaa aaatgcagtt ggactagcaa ctatcgctat tttgtcaaca
    17941 atggttttgg taaccatgtc agcagcgaca agcattttca attccgcaga atcctttaaa
    18001 aaagttctaa atcctcatga ttttggggtt tcaggacaaa atgttgaaaa agaagatttg
    18061 gacaaactct tgagccagtt tgcaagtgac aatggttata agattaaaga aaaagaagtg
    18121 tttcgttaca cttactttgg tgttgcgaac caagaaggaa ataagttaac cttttttgaa
    18181 aaaggacaaa atcgtgtcca acccacaaca gttttcatgg tatttgacca aaaagattat
    18241 gaaaatatga ctggtcaaaa actgtctcta tcaggaaatg aggtcggtct ctttgccaaa
    18301 aatgacggac tgaaaggaca gaaagctcta actctaaatg atcatcaatt ttctgtcaaa
    18361 gaagaattta ataaagattt cattgtcaac catgttccaa ataagtttaa tatcttgact
    18421 actgattaca attaccttgt tgttcctgat ttacaagcct ttttggatca attcccagat
    18481 tcggctatct ataatcagtt ttacggtggt atgaatgtag atgccagcga agaagaacaa
    18541 ctcaaggtcg ctgaggagta tgaaaactac ctcaatcaat ttaatgctca attagacaca
    18601 gaaggtagct atgtttatgg tagcaatcta gcagatgcta gttctcagat gagtgccctc
    18661 tttggtggtg tcttctttat cggtattttc ctatccatta tctttatggt cggaactgtt
    18721 ctggtcatct actacaaaca aatttctgaa ggctacgaag accgtgaacg ctttattatc
    18781 ttgcagaaag tcggtttgga ccaaaagcaa atcaagcaaa ccatcaacaa acaggtttta
    18841 actgttttct tccttccttt gctctttgcc ttcatacatc tcgcctttgc ctaccatatg
    18901 cttagcctga ttttaaaagt gattggtgta ctggatacga ctatgatgtt gattgtgacc
    18961 ttgtctatct gcgctatctt cctcatcgcc tatgtgctga ttttcatgat tacttcaaga
    19021 agttatcgca agattgtgca aatgtaaaaa agatacctcg acttcaaaat cgaggtattt
    19081 cttgtattct aaatgctgaa aagttgttcg agcaggaagg taactcccat ggtcaagaga
    19141 ccaatagcaa ggttccgaat catagctgtt ctagtcggat cctttccaag tctggcacta
    19201 gtgtaaccag tgagaagaag ggccacaccg acaataagga cagtagcagg gatgcgatat
    19261 tcacttggga atatggtcac tgacagcatt ggaggcaaac ttctaaggaa aaaggcaacg
    19321 aagctagaaa tggcagcgtg ccaaggattg gtaaattctt catactcaat cccatatttt
    19381 tcctctacca gagccttgag tggattttta agaaagatct tattggtcaa gagttgggca
    19441 gaagttttgc attctccatt ttggatataa gcagcataga gggatttttt ggctagttcc
    19501 ctatcttggt ctaacaagag tttttctcgc gaaacggcag cttcctcggt atcttttgga
    19561 gttgaaacgg atacatattc tccaccagcc attgaaaagg caccagctaa gatagccgca
    19621 aatcctgata aaaagataat ccagatattg gtcgtggcac tggcaacccc gataaccaca
    19681 ccagcaatgg aaataattcc atcgttagca ccaagaacac ctgcgcgcag gatatttaaa
    19741 cgacctgcaa aatttgaatc gatttcgtga tttgtttctg acgctaaatt tcaagttcaa
    19801 gttagccatc cagaagtctt ctctgggtga cttgtagtcc aagcattttt taggatagtt
    19861 gttaatccac ttttcgatga atgcgacttc tttgggagtc attttcttgg ttcccttagg
    19921 taaccatcta cgaatgagcc tgttgtgatt ctcattagtt cccctttccc aagaggcata
    19981 ggggtgcgca taatagatgt gctcctcaga aaatacatca aacaagcgat tgaattccgt
    20041 tccattatct gccgtgatgg aaagaatctt gtgttgtttt aagatgagtt ttagagcctg
    20101 attgaccacc tcagcacttt tatttggaat caatcggatg atctgatgtc tactctttcg
    20161 atccgtcaag acaagcaagc agtagttttt cgctctcgta agtagaaccg tatcaatctc
    20221 ataatgccca ttctccaagc gaagattgat agcttcagac cgctgttcga tagattgacc
//
LOCUS       DBERCA010000033        19874 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.033, whole genome shotgun sequence.
ACCESSION   DBERCA010000033 DBERCA010000000
VERSION     DBERCA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.033"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(112..387)
                     /locus_tag="ACL719_001313"
     CDS             complement(112..387)
                     /locus_tag="ACL719_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGS0699273.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(493..1332)
                     /locus_tag="ACL719_001314"
     CDS             complement(493..1332)
                     /locus_tag="ACL719_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0699274.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1474..2322
                     /locus_tag="ACL719_001315"
     CDS             1474..2322
                     /locus_tag="ACL719_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS0699275.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2415..3332)
                     /locus_tag="ACL719_001316"
     CDS             complement(2415..3332)
                     /locus_tag="ACL719_001316"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGS0699276.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWY"
     gene            3809..3958
                     /locus_tag="ACL719_001317"
                     /pseudo
     CDS             3809..3958
                     /locus_tag="ACL719_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(4284..4577)
                     /gene="rpmA"
                     /locus_tag="ACL719_001318"
     CDS             complement(4284..4577)
                     /gene="rpmA"
                     /locus_tag="ACL719_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGS0699277.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4594..4938)
                     /locus_tag="ACL719_001319"
     CDS             complement(4594..4938)
                     /locus_tag="ACL719_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGS0699278.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4954..5268)
                     /gene="rplU"
                     /locus_tag="ACL719_001320"
     CDS             complement(4954..5268)
                     /gene="rplU"
                     /locus_tag="ACL719_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGS0699279.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5305..5392)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5487..6086)
                     /locus_tag="ACL719_001321"
     CDS             complement(5487..6086)
                     /locus_tag="ACL719_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGS0699280.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSVYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6557..6799
                     /locus_tag="ACL719_001322"
     CDS             6557..6799
                     /locus_tag="ACL719_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGS0699281.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALE"
     gene            7076..8089
                     /locus_tag="ACL719_001323"
                     /pseudo
     CDS             7076..8089
                     /locus_tag="ACL719_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8405..9379)
                     /gene="pta"
                     /locus_tag="ACL719_001324"
     CDS             complement(8405..9379)
                     /gene="pta"
                     /locus_tag="ACL719_001324"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGS0699282.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(9423..10319)
                     /locus_tag="ACL719_001325"
     CDS             complement(9423..10319)
                     /locus_tag="ACL719_001325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0699283.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSYIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(10316..11134)
                     /locus_tag="ACL719_001326"
     CDS             complement(10316..11134)
                     /locus_tag="ACL719_001326"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGS0699284.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(11118..11789)
                     /locus_tag="ACL719_001327"
     CDS             complement(11118..11789)
                     /locus_tag="ACL719_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS0699285.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRSITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            11917..12486
                     /locus_tag="ACL719_001328"
     CDS             11917..12486
                     /locus_tag="ACL719_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGS0699286.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTLDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            12599..13558
                     /locus_tag="ACL719_001329"
     CDS             12599..13558
                     /locus_tag="ACL719_001329"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS0699287.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            13568..14683
                     /locus_tag="ACL719_001330"
     CDS             13568..14683
                     /locus_tag="ACL719_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS0699288.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMH
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            14688..15035
                     /locus_tag="ACL719_001331"
     CDS             14688..15035
                     /locus_tag="ACL719_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGS0699289.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            15043..15258
                     /locus_tag="ACL719_001332"
     CDS             15043..15258
                     /locus_tag="ACL719_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS0699290.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            15341..15982
                     /locus_tag="ACL719_001333"
     CDS             15341..15982
                     /locus_tag="ACL719_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGS0699291.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            16000..16689
                     /locus_tag="ACL719_001334"
     CDS             16000..16689
                     /locus_tag="ACL719_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS0699292.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGKPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(16686..17366)
                     /gene="radC"
                     /locus_tag="ACL719_001335"
     CDS             complement(16686..17366)
                     /gene="radC"
                     /locus_tag="ACL719_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS0699293.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHLSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(17409..19700)
                     /gene="pcrA"
                     /locus_tag="ACL719_001336"
     CDS             complement(17409..19700)
                     /gene="pcrA"
                     /locus_tag="ACL719_001336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGS0699294.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLNITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSVLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
BASE COUNT         6148 a         4183 c         3580 g         5963 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaattgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt ttttaggagt ggtaacgtat caaaatagcc caagcgttct cacccgtgtg
      541 agtttgaata atggaacccg tttccaaaac agaaattggc ttttcaacat aagcttgtaa
      601 gctttctttc atctcttttg cccaatcatc actaccagaa tatgaaattc caatctctgc
      661 tacagcacgt tcagaaagcg atgttatcaa ctcatctaac cattttttaa atgttttagt
      721 tccacgacct ttaaccattg gctgcaattc atggtctttc atttgcatga caacacggat
      781 attgagaagg gagctcaaca agccagttac acggccaatt cgtccacctt tgacaagatt
      841 ttccaaagtt gaaacaccaa tataaagctc tgtatggttt ttaacctctt ctacatgaga
      901 taaaattgcc tccatatctt taccttcttg agctaacttc gcagcctcaa caacttggaa
      961 tttcagggct tggtcagtga aggaactatc aacaacagtc acatctgcag tagataggct
     1021 agcaccttgg cgtgctgctt ctaccgtacc cgaaagagca tgggacatat gaatagcaag
     1081 aatctggcca ccatctttgc ataggtcttc aaaaatctca gcaaagacac ctacaggtgg
     1141 ctgacttgtt ttcggaagat tcttactttc ttgcatcaac tgaagaaatt taccttcttc
     1201 tttcaaatcc gcatcagaat aaacaacatt atcaatcatt acagataatg gaacaattgt
     1261 aatatctaat tgctttacta gttcaggttc aatagtaaca gatgaatcgg ttacaatctt
     1321 aatttttgtc atagtatcaa tctttctatt ttaggattca gattggtttc cttacttcta
     1381 attatatcaa aaaaaagatt aaaaatccta atggagtcaa tcaaattttc cgtaaaattt
     1441 gatataatca acttataaga aaagaggtgt cctatgatta aaaaaattta ccccattttt
     1501 accattttac taggtgctgc tatttatgct tttggactga cttattttgt agttccccat
     1561 catctctttg aaggaggggc gacaggcatt accctcatca ccttttatct ttttaaaatc
     1621 cctgtttccc tcatgaacct gctgattaat attccccttt tcatcctagc ttggaagatc
     1681 tttggagcca aatccctcta ttctagttta ctaggaacct tagctttgtc cgcctggtta
     1741 gctttttttg agcatattcc ccttcatatt gatcttcaag gtgatttact aatcacagcc
     1801 cttatagcgg gaatcctatt gggaattggc cttggaatta tttttaatgc tggaggtaca
     1861 actggcggaa ctgatattct agctcgtatt ctcaacaaat acactcatat atccatagga
     1921 aaactgctct ttatcttaga tttttgtatt ctcatgttga ttctcctaat cttcaaggat
     1981 ttgagattgg tttcctacac gcttttgttt gattttattg tttctcgtgt tattgatttg
     2041 attggtgaag gaggatatgc cggcaaaggc tttatgatta tcacaaaacg tcctgaccaa
     2101 cttgctaagg cgattaatga tgacctcgga agaggtgtta cttttatttc tggtcaaggc
     2161 tactatagta aagaaaattt gaaaatcatc tactgtattg tcggaagaaa tgaaattgtg
     2221 aaaacgaagg aaatgattca tcgaatcgat cctcaagcct ttataactat tacagaagcc
     2281 catgaaatcc taggagaagg cttcaccttt gaaaaagaat aaaaagaggt aatgtcgtga
     2341 cctcaaaagt tagactaaat catctatctt ttgggttaca gacaacctct tttttatttt
     2401 atttactcaa gctcttaata ccaattccga gttacttctt catcagcctt taactgatcc
     2461 actaattggt caactgagtc aaatttggtc atatctcgaa tgcgatcaag ccaataaacc
     2521 atgacggttt ccccataaat atcttgatta aaatcaaaaa tattgacttc aaaacgtgct
     2581 tcttctccat caaaggtcac atttttcccg acactagcca tagcacgata cttctgtctc
     2641 tgaatctcaa catcaacaac ataaacgcca tctgctggca tataagtacg gtctaaaagc
     2701 actaaattcg ctgtcggata accaattgta cgaccacgag cattaccatg aaccaccata
     2761 cctcttgatg gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt tccatctaaa
     2821 atagcttgac ggatacgagt tgaactaatc tttcctttct catcttctac aggtggaaca
     2881 atgataactt ctccatcaaa gtaatccttt aaatcttctg ctgttttttt gtcagaacca
     2941 aatgtataat caaaacctgc aacaataatt ttggcattca tagccttgat ataagttgca
     3001 aagaattctt gtgcagtgag actagcgaat tgactactaa aatcaaggag atataattct
     3061 tctacacctt cgcgctttaa ttttctttca cgttcagcag ggttcaaaat atgcaaaaac
     3121 aaatctggat gataaggctc taaagcgatc tttggagatt cattaaaggt cataacgacg
     3181 ataggcaaca aatcctttct cgcagccttg ttggcaacac gaaataattc ttgatgcccc
     3241 ttatgtatgc catcaaaata gccgagaaca acgactgaat cagatggtgt gccaatatct
     3301 ttttggtttt ttataggaat agtaataatc ataaaataat tatatcatag cgatagctat
     3361 ttctggaaca gaaaatctga aatgttgttt ttttcacatg aagtgtacct gttttcaaaa
     3421 agcactttat tctatcgttg cttaactatg aactttgcaa tattcttctc aaaaacttgt
     3481 aggacatctt caaaattttg caaggagtga ttagacttgt tcggtaacca taaagtgtca
     3541 tactatgctt atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat
     3601 tggtgccgaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa
     3661 tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc
     3721 actttccctg atgacaaaaa aagccaaagc agtaaagaaa aactagatga ctgctttcca
     3781 acaacttcca tctagtgtgc ttcagactgg gctatttttc tctccatctg ttagcttgga
     3841 ttctcagacc gtttcagcta aagaatatct tttcccttat cagaaggaac gggctcaaac
     3901 cactcagaca agtgaaggga cgacaagcca atatttgaaa ccagatagca gtttttatag
     3961 tcaattgaaa taaaatctga agaaatcgag taggaaactc atatcaattt ttaacagtgt
     4021 tctattccag attcatactc aatgaaaatt aaagtgcaaa ctaggaagtt agccgcaggc
     4081 tgtacttgag tacggcaagg tgaaactgac gtggtttgaa tttgattttc gaagagtatc
     4141 aatccactat acatattaaa aagtatgggt atcaaaacaa aaaaacaggc tctccgaaaa
     4201 ctcggaaagc cttattttaa gctgtttcta gcttcctcac ctttacattt caaggttcgg
     4261 gataaaaggt tcaatggacc tttttattta gcgattgggt aaacagaaac ttgtttttta
     4321 tcgcgtcctt tacgttcaaa gcgtactacg ccttcaactt tagcgaacaa agtatcgtct
     4381 ccaccacgac caacgtttac acctggatag atgtgtgtac cacgttgacg gtaaaggatt
     4441 gatccacctg ttacagtttg tccgtcagct gctttagctc caagacgttt cgcttgtgaa
     4501 tcacgtccgt ttgatgtaga acctccacct tttttgtggg cgaaaagttg caagttgtta
     4561 agagtcattt ttaacataat gttttcctcc gtgttagttt tctgtgataa ctctggtttg
     4621 gacgaactca gaagagttct ccgataagtt tgccatacct aagaaaaatg attcaaagaa
     4681 taactgggtc atttctctct ggtgtgaagg aagatctttt ggtatttcaa ccatcagata
     4741 gccaccttca tcttcgttta attctaggat tggttcatag cctgcaaatt tctcaataga
     4801 attgataaag ttaatggcaa gcgtagaaac cgatgcacac acgacatcta agccgtattc
     4861 gccactctcg gcgtgtccag taatttccgc actcctcagc tcgccatctt cggctctctc
     4921 aaagactgct tgtatcatgt gttctcctta aaattaagcg ttaattgcgt tgatgacaac
     4981 ttttgtatat ggttgacggt gaccttgttt acggtggcta ccttttttag gtttgtactt
     5041 gtaagtaaca actttctttt gttttccttg tttttcaaca gttccaacta cagtagctcc
     5101 agcaacaagt ggagttccga caacagtgtt ttcaccacca acaagaacaa cttcgttaaa
     5161 agtaacttct tgaccagctt caacgttcaa tttttcaatg taaactgctt gaccaacttc
     5221 aactttaact tgttttccgc cagttttgat aattgcgtat gtgctcatta tgcacctcct
     5281 atgattttta tgggtttccc cgattttttt gtgaagactc gcctagcatc gtgggacaaa
     5341 ccacttaaac ttgaataatc aagcaacgat gttcgtgcgg ttgcacagga tcgtgcatag
     5401 tcaactcttc aagtgtagca tatttcctat tttcttacaa gtaataacac ctaaaatcaa
     5461 gctttttctt ttactttttt ctgccactag gcaaaaagca tactgagata aaaaatgctc
     5521 atcataatag gaacaccaag aatggtcttt tcatgataga aaatcgtcaa atagactgaa
     5581 aagacaacgc caaggacaaa actactaagc aggctaacaa atatgaaccc ttcacgcaaa
     5641 aaaggagtgt gcttagttcg gaaataatct ccaaaagcta gcattgttcg tttgatattc
     5701 cctgtcataa aggcattatt gtaggcaata cccgaaacct caccaaaagc agttgtcacc
     5761 agtcccatac agaaggccaa gggcggcact agatagatat tatctacagt ttgtggcaca
     5821 aaaccaataa ttattgatag gattgctaaa ggaatcaagg acagaataga ttttttcaca
     5881 attctcaatt tttccttata aatcgttaat aaaaagactc ccatcataaa cgctattaag
     5941 gttaaaagct tatccctaat atccgaaaca ttattttgta tgagctctac cgaaagaaag
     6001 acaacatttc cagcttgtcc agctacaagg gtattcccgc gaacaataaa agtgtaagca
     6061 tccacatatc cagcacaaaa cgtcaaaaaa gtgctaacct tttagactga cgtgatattt
     6121 ttcttatagg taataacctc attttccctc ccattgtatt ttctcttaga aatattgtac
     6181 cattttcttt ctaaaaaatc gtaggctacc atttagattt tactattagc ataaaaataa
     6241 taatagacaa ctatttatcc aaaaatagat agaggtaaca tgtttgcaaa caaagcatac
     6301 gaacctttag taaaatcatt tccatgaaac tagaatagag cccctcttag caaaaatcat
     6361 tattttaatt tatttctaat cactccttga cataaataac tctcaccaat aaaagactat
     6421 gtcttaaaaa aatggtataa taaaatcaat acttggactt gatggctatg ctactaataa
     6481 caattaggag agaaaatcag gcacttgtta acaacaagga ttatcccctt gagatgaaag
     6541 gaactttaga aatcttatga tgaacatgca aaacatgatg cgtcaagcac aaaaacttca
     6601 aaaacaaatg gaacaaagcc aagctgaact tgctgctatg caatttgttg gcaaatctgc
     6661 tcaagatctt gtccaagcaa ccttaactgg cgataagaaa gttgtcagca ttgatttcaa
     6721 tccagctgtc gttgacccag aagaccttga gactctttct gatatgaccg ttcaagccat
     6781 caactctgct cttgaataaa tcgatgaaac tactaagaaa aaactgggtg ctttcgctgg
     6841 gaaattgcca ttctaaaaac aaggagctag aacaatgctt gtcgataaca aaggctaaga
     6901 aaggtgcaaa aattataaaa aacgcatagt atcaagcaac ctaaaacttt gggctctata
     6961 atatttgtag tgggtaaatc ccctatggat attacggagc ctatttttgt gtagaaaaaa
     7021 agtcccatat gacctataat gaaaagcgac aaaacaactc attagaaaga atcatatgga
     7081 acaattacat tttatcacaa aattactaga cattaaagac cctaatatcc agattttaga
     7141 catcatcaat aaggatacac acaaggaaat catcgccaaa ctggactatg aagctccatc
     7201 ttgtcctgag tgcggaaacc aattgaagaa atatgacttt caaaaacctt ctaaaattcc
     7261 ttatcttgaa acgactggta tgcctactag aattctcctt agaaagcgtc atttcaagtg
     7321 ctatcactgt tctaaaatga tggtcgctga gacttctatc gtcaagaaga atcatcaaat
     7381 tccttgtatc atcaaccaaa agattgatca gaagctaatt gaaaagactt ctatgaccga
     7441 cattgatcat cagttgtcta tttcaacttc aactgtcatt cgcaagatca atgattttca
     7501 ctttgagcat gatttttcgc gtcttcctga gattatgtcc taggacgttg aaacagtctg
     7561 gggagtgact gtttcaatcg ggagatagag atgagcttta ttgctcagga ctttgataag
     7621 ctcaatatca taactgttct tgagagcaga acacaagcca tcatccgaaa tcccatgaat
     7681 acaaggctat caagcgatac tgaaagctca ttcaacaaga tagtcagaaa ctgagtgata
     7741 aacgatttta tcgccctact tttcgtatgc acttgactaa taaagaaatt ctagataaac
     7801 ttttgagcta ttccgaagac ttgaaacacc actataatct ctatcagctc ttgcttttcc
     7861 acctcttttt cagactctgt ttaaaacctt cctcaaagat aaagagaaaa tcgtcaacgc
     7921 ccttcaatta ccttattcca acgccaaact ggaagccacc aataatctca tcaaacttat
     7981 caagcacaat gcctttggtt ttcgaaactt tgaaaacttc aaaaaacgga tttttatcgc
     8041 tctgaacatc aaaaaagaaa ggacaaaatt tgtcctttct cgagcttagc ttttcttcaa
     8101 cccactacag ttgacaaaga gccagaaaac gcataatagc aggtgtcaag acacttaata
     8161 ttatgcgttt tctcatgaaa aaattctctt ttcatactca tttaaaatca aaaagggaat
     8221 ttatcgtttt acggacatgt atattacaga tttctatagg tcgtgtatag acaggatatc
     8281 atcgcttgga agttttcctt tttgacacca atcagtaatt tagttctcag gaattaatgg
     8341 cagtgctttt cgtacatagt ttcataacta cagtatagca tagtatatcg ctttatagtt
     8401 ttcactattg atgaactgct tgagctgctg tgatgagggt caacttgtaa acatcatctg
     8461 cattacatcc acgagaaaga tcattaactg gcttgtttaa accttgcaaa acaggtccta
     8521 cagccgcaaa gccacccagg cgttcagcca tcttgtaacc gatatttcct gcctcgatac
     8581 ctgggaagat gaagacattt gcttgaccag ctaccgtact tccaggagct ttcagagctg
     8641 cagtttcagg aacaaaggct gcatcaaatt gcaactcacc atcgatttca aggtcaggac
     8701 gcaagtcgtg agcaatttta gttgcttcaa cgaccttatc aacgctttca ccaaaccctg
     8761 aacctttagt agaatagctc aacatggcaa ttttaggttc gatgccaaac atcttagctg
     8821 tgattgctga gttgatggca atttcagcca aggcttctgc atctggattg atgttaatgg
     8881 cacagtctcc aaataggtaa cgttccgtac cacgaaccat gaggaaggct cctgaagtac
     8941 gagttacatt tggacgagtt ttgatgattt gtagagctgg acgaactgtt gaagctgttg
     9001 agtgaatcgc tcctgacacc attccatcaa ccaagcccaa gtaaaccaac atcacaccaa
     9061 agtagttgac atcttcaacc aaaaccttgc gtacatcttc ttcagtcatt ttgcccttgc
     9121 gacgctccac caaggcagaa accatttctt caaattgagg ataatgttga gggtcgatga
     9181 cctcataacc atccatgatt ccttcaattt caagataaat tttaattttt tcaggatttc
     9241 caagcaaaac aggaatcact tctgtttctt ttactaagcg ttttgttgct tgaagaatac
     9301 gaggctcttc cccttcaggg agaacgatac gagcattttt accaacaagg ttggctttga
     9361 gactttcaaa aacttccatg agttttctcc tttaagataa taattatact ctgagacagt
     9421 ttttatagag tattagttga taactgggta ataaggttac tgaaatcatc cggcaaggga
     9481 ctttctaact gcaagtcttg ctctaaaaat ggatgataaa aggataggta atggcaatgc
     9541 agagcctgac gttgaatacc atcttccaga ctaccaccat acaaatcatc tcccagcaaa
     9601 ggaaaaccga tataagaaaa atggactcgg atttgatggg ttcgaccagt gtgcaggtga
     9661 atatagacca agtgaatatt tccataagaa gctacaatct tgtatgaagt atgggcatac
     9721 tttccgcctt tagccactcg tctggtaata atggaatctt catcacgcgc aatcggagca
     9781 ataatttccc cttctggctc caaatgtcca tctcccttaa ccaaagcaaa gtagcgtttc
     9841 tcgatagatt tcttctgcaa ctgcttgtct aatcgtgcat gggcataacc gtgcttggca
     9901 aagagcatca agccagaagt gtccctatct agtctggtaa caatgtgaac ctgctgattt
     9961 tcataatttt gcttgacata gtaacccttg ataaaattgg caatggtatt agagtgattg
    10021 acactaggaa tagaagccac tccatagggt ttattcaaga ctagaaagtg gtcatcctca
    10081 tagagaatat ctaatggatg ctcaatagcc tccaaggttt caaagccttc ctcagcggga
    10141 atgtaaatgg taacgtagtc tccaacgtcc aatagatacg ttgcattttg cggttgatta
    10201 ttgaccagaa tcgctccacc tcgaaactta atcttagcca gcaatccctt agaaacctcg
    10261 tgctttttta aaaaggtctt aaccttgaca tgttcatctg cgataaattc aaacctcatt
    10321 catccacctc accgataaag gcatccttaa cacggttcca aaaactggta tggctaggag
    10381 tcgcgacaaa gtgaatctta tgatggtcga tttgatactc aatacgctca atattacgga
    10441 aagaataaac gctattgtca accgaaatag tatgataatc gtttcttgtt ggaataagtt
    10501 caatcttatc cttcttaggc acaataatgg aagagcccag tgttcgataa acacgattat
    10561 taaggctggc aatttccgtt aattgcaaag cttcaatggt agggtgtaaa acagcaccgc
    10621 caagagactt gttataggca gtactaccag tcggtgtcga aactgttagc ccgtctccac
    10681 gaaaacgttc aaagggaaca ccatttatta caatatctgc caccatggtt cgatcagacc
    10741 tgcggatgct ggcttcgttg agtgctctga aaatcttaac ttcaccattt tcaagaaaga
    10801 ccttcacatt cagaacaggg taagaaaccc ttgccccagt atctagctgc aaattagtca
    10861 ctagcttgtc caactcaaaa tcacgataat ctgtatagaa gcccaaatgt ccagtatgaa
    10921 gaccgataaa gcggaccttg tcaagctgat tttcgtactt atgaaaggcc gacaagagca
    10981 taccatcccc gccaatggaa atgacaatat ccggattggt atcattgagt ataaactgat
    11041 ttctcttcaa acgatctcgc aattcataca aaaccctttg actctgcggt tttctattgg
    11101 ctatcagatc aattcgttta cctgtattct tcatctgtat cgtcactgtt tcctacaccg
    11161 tcatttaatt ttctactcaa aggatcaaaa agtgcctggg cttcttggat atcatcacga
    11221 atttcaccca tttcttcatc caactgatgg gcgattctag ctgtaatttc cagtcgcttc
    11281 ttaatctcat ctgggaaatc cccttggtac ttgtagttga gagaatgttc tatcgttgcc
    11341 cagaaattca tggccaaagt acgaatttga atttctgcca aaatagtctt agctccattg
    11401 atggtatcaa ccgtatattc tactaccaca tgataggaac gatagcctga tgcttttcta
    11461 tgagtaatgt aatctcgctc ctgtatgatt cgcatatcct gacgcttgtg caaaatatcc
    11521 actacttcct tgacgtcatc tacaaactga accatcacac gtaagccagc aatatcctgc
    11581 aaatcgtgtt ccaaggtcgc ataagtaatg ctacgacgag ccattttttc tttgatgctc
    11641 tcaattggct tgactcgacc ggtcacaaac tcaattggag aatgcttatt ttgcttacga
    11701 tattgcttac gaataccacg aagtttaatc tttaactcac caacagcttg aatgtaagga
    11761 tctagaaatt cttcccattc taaggtcata tattctccct tgttctcata ttatccgtca
    11821 aaaatatctt tgattttttc tccagattca tatacaataa atacaatgct tattatacca
    11881 taaatccgct ttcaacgata aaaaaaggta agaaaaatga aacatttaga aattgaattg
    11941 aaaacactat tgaaaaaaga tgaatacaat cgtctaaaag accagttcac aggtgtcact
    12001 cctgttcttc aaacaaatta ctacatcgac acgcttgatt ttgaactgcg agaaaagaaa
    12061 gtcgctatgc gcattcgaac ctttgaagac tgggctgagt tgacactcaa agtcccgcag
    12121 agtgttggaa acatggaata caaccaaaaa ttgcaactaa aagatgctga gaactatctg
    12181 agtaaggaag aacttcctca agggctagta ctggatgaat tggcgaaaca tggtatccaa
    12241 agtaagaact ggcaggttct tggttgtcta acaacgcttc gctatgaaat gaaaacagct
    12301 attggtctca tggcactgga tcagagccga tactttgata tgacagatta cgaattggag
    12361 cttgaagtgg aaaatcatga gcaaggcaaa caggatttcc gacaattttt agagaaaaat
    12421 cagatttcct accaaaaagc tccttcaaaa ttggttcgat ttgtcaaaag tatgaaaaat
    12481 agctgaaata atctccattt tttggtaaaa tagaaaagat aaaaatacac aaatcccagt
    12541 tcatatatgt aaggtaaata taactaggat ttataaagtt tacagaggac ggtctataat
    12601 gtcagataga aaaaacatga aacttttcgc actcaactct aaccaagaga ttgcacaaaa
    12661 aattgcccaa gctgttggtg tcccacttgg aaaactatca tcacgtcaat tttcagacgg
    12721 agaaatccaa gtaaatatcg aagaaagtgt ccgtggttat gatgtttaca tcatccaatc
    12781 aacaagtttc cctgtcaaca accacctaat ggaattgtta atcatggtcg atgcttgtgt
    12841 gcgtgcaagt gcccacagta tcaacgttgt ccttccatat tttggctatg cacgtcaaga
    12901 ccgcattgct tgtcctcgtg agccacttac agcaaaacta gttgccaata tgctggttaa
    12961 ggctggagtt gatcgtatcc tgactcttga tttgcatgcc gttcaggttc aaggtttctt
    13021 tgatattcca gtggataatc ttttcactgt tcccctattc gcaaaacatt actgcgataa
    13081 aggattgctt ggttcagatg ttgttgtcgt tagccctaaa aattcaggtg tcaaacgtgc
    13141 gcgtagcctg gctgaatatc ttgatgctcc tatcgccatt atcgactacc ctcaagacga
    13201 tgcaactcgt aacgaaggtt atattattgg tgatgttgaa ggtaagaaag ctatcttgat
    13261 tgatgatatt ttaaatacag gacgtacctt ctctgaagct tctaaaatcg ttgaacgtga
    13321 aggagctaca gaaatttatg ctgtttctag ccacggtctc ttcgtcgagg gagctgctga
    13381 gcttcttgac aatactaata ttaaagaaat tcttgtgact gattcagtag caacaaaaga
    13441 aaaaactcct aaaaatgtat gctacatcac tgctagtgag ttaattggtg atgctatcgt
    13501 ccgtattcac gaaagaaaac cagtcagccc actctttgcc tacaataaaa agaaataagg
    13561 tgattctttg atttatttgg acaatgctgc aacgactcct atgtcagcag ttgctatttc
    13621 agctatgacc aaggttatgc aagaaaccca cggaaatcct tctagtattc atggtcatgg
    13681 tcgtcaagct ggcaaactct tgcgagaagc ccgtcaggaa ctagcccagt tactaaggac
    13741 aaaacctcaa catatctttt tcacttctgg tgggactgaa ggcaataata ctaccatcat
    13801 tggctactgt cttcgtcacc aagaacaagg aaaacatatc atcacaactg ccatcgagca
    13861 ccatgctgtc cttgaaacaa ttgattactt ggttcaacac tttgggtttg aagcaaccat
    13921 tatccagcca gaaaatcaag aaatcacagc ccagcaaatt caaaaggctt tacgtgacga
    13981 tacgattttg gtttctacta tgtttgccaa taatgagaca ggaaacctac ttcccatcgc
    14041 tgaaattggc caaatactca agcaacaccc tgctgcctat catgttgatg cagttcaggc
    14101 tattggtaaa atcccaattc attcagaaga attgggcatt gattttctca ctgcttctgc
    14161 ccacaaattc catggtccta agggaatcgg ttttctctac gcatctagca tggactttga
    14221 ttcctatcta catggcggag atcaggaaca gaaaaaacgt gcaggaactg aaaatctgcc
    14281 tgccatcgta ggcatggttg cagccctaaa agaagaccta gaaaaacaag aagaacattt
    14341 tcaacatgta caaaatctag aaactgcctt tctggcagag ctggagggca ttcagtatta
    14401 cttgaataga ggaaaacatc atctccctta tgttctcaat attggatttc ctggtcagaa
    14461 aaatgacctc ttactccttc ggctagattt agctggaatt tcaatctcta ctggctcagc
    14521 ctgtactgca ggcgttgtcc aatccagcca tgttcttaaa gccatgcatg gcgcaaattc
    14581 agaacgcttg aaggaatccc ttcgcatcag tttgtcgcca caaaataccg ttgaagacct
    14641 acaaaccctc gcaaaaacct taaaagaaat tatcggaggt tagccatatg gcatttgaaa
    14701 aaatcattca gttaaaaaat tgtcgttacg attacactct tagcccttct gttaaaaaat
    14761 tcaccctcaa agataacacc ttttttgaaa ctaaggttgg taactatgaa ctgactcgcc
    14821 ttttggaaaa agtgccaaac agcggtgaag gcttccaact caaaatcatc attaacaagg
    14881 aacttacagg ggctaaaatc aatatcactg acaagtttgg ccttcgtcta gttgatattt
    14941 tcaaatcaga agaccaccat attcatcagg aaaaattcta cttcctcatg gatagcttgg
    15001 tagaacgtgg tgtctttaca aaatcggaaa gatagagcca aaatgtttga actgacctat
    15061 aaggactgct atcatgtaga gcgtactctc aagtatgagg atcatgaggc tctcatgttg
    15121 actttgtcag gatgtgtaac cttaccagat acactttatg tgacttctct gacttttagg
    15181 ggcaaaaaag tttatcaggg gctggtcgga gacctctacc gttttctatc tcatgcagat
    15241 tttttacatc aaaactaaga tttttcttag ttttttctta tttttgttga ttttttcaca
    15301 atgtttctat aaaatagaag aatagaaagg ttgtgatttt gtgaaagata aacagtttgc
    15361 tattccaaaa gctacagcaa aaagactctc tctctactat cgaattttta agagatttca
    15421 tgcagaaaag attgaacgtg ccaactctaa gcaaattgca gaggctatcg gtattgattc
    15481 agcgaccgta cgtcgtgatt tttcctattt tggtgaactt ggtcgtcgtg gttttggcta
    15541 tgatgtcaaa aaattaatga cattttttgc cgatttgctc aatgacaact ctattaccaa
    15601 tgtcatgctg gttggtattg gaaatatggg ccatgccctt ctccactacc gcttccacga
    15661 acgtaacaag atgaagatta tcatggcctt tgacctagac gaccatcctg aagtcggtac
    15721 ccaaactcct gacgggattc ccatttacgg gatttctcag atcaaggata aaatcaagga
    15781 tgctgatgtg aagactgcta tcctaactgt tcccagcgtc aagtcacaag aggttgctaa
    15841 tctcttggtt gatgctggtg tgaaaggaat tctcagtttt tcaccagtcc atctgcattt
    15901 accaaaagac gtggtcgttc agtatgtcga tttgacaagt gaactccaaa ccctcctcta
    15961 cttcatgcga aaagaggatt agaaagcgaa aatcatttta tgaaaaaacc agttattggg
    16021 attacaggaa acgaaaaaac tcatccagat gatgacatca tgatgagcta cgcagcaaaa
    16081 ggctttgttg aaggcgttaa agatgctgga gggattccta tcatcctacc gattggtgat
    16141 caagaaatgg cctgccacta tatcagtttg attgacaagc tcatcttgac aggtgggcaa
    16201 aatgtcgatc caaaattcta tggcaaaccc aaaaccattg atagcgatga ttatcacctt
    16261 caaagagata tcttcgaact ggccctcatc aaggaagcta ttaaacagaa aaagcccatt
    16321 ttctctgtct gccgtggtac ccaactcttt aacgttgcca tgggtggaac tttgtaccaa
    16381 gatatcgaag accattggca ggattcttct gttgagtaca caactcaacg cttggtaaca
    16441 gaaccagata ccgttctcca agaaatctat ggagaaatct ctcatatcaa ctccttccac
    16501 caccagagca tcaaggactt agcaccaaat ttaaaagttg tggctcatga tcctaaagat
    16561 ggtatcattg aggctgtcat gagtacggat gatgtcgcct ttctcggtgt ccaatggcat
    16621 ccagaacttc tttttgaaaa tcgtcccaaa gataagaaac tctttgacta tgtcgttaat
    16681 gaactttaga ttaaatctgt cttttcacga taactaaagt aattagaatg agagacaatc
    16741 aaatggtcca agagaacaat ccccatcagt tcgcaggctt ctttaacaag tttagtgaca
    16801 tgatcatcat tttggctagg cgctaccgct cctgaaagat gattgtggac caagataaga
    16861 gaagtcgcca tatgcttgat tgcatagtgc agaatctctc gcggttcagc gatactacga
    16921 gttacagaac cgataaaaat ggtctgctga tggatgattt gattttgagt attgagatag
    16981 agtgccacca ggtgctcttg ttttttatcc cctaattcct gctgcatctt cttggccaac
    17041 ttttgactgc tgagaatact ttccatttca agagtctcgt gtttgtgaat acgatgcccc
    17101 agttcaatca tagcttgtaa ttctatggcc ttaacacgcc caataccaga caaactctgc
    17161 aattcctgca gggtcatttt tttcaaatcc gttaggcttg aaagattgtt caagactttt
    17221 tgggcaattt caaaaacgct agcttgacgt gttcctgtcc tgagtaaaat agctagcaac
    17281 tcttggttac taagcgcttc aactccttcc ttggccagcc tttctcttgg taatagtgaa
    17341 tcttcttgga atgaaatact gtacataaaa atcctcctca ctttattatt cgtgagaagg
    17401 atggaaaatt agattttttt ctcaattgga gccacactgg ctaaaagttt tttcaaacct
    17461 acttctggga aattgatttt caattcctgc ctagcaccgc tacctgaaac ttccagaaca
    17521 gttccctctc cccatttctt gtggagagca atgtcaccaa tggaccaatt tgcctcgcta
    17581 gatgctgatt ttgcgccagc tgtaaattga ccaaatggaa gaacgcttga ctggattgat
    17641 tttggggcag caccgcgttt acggtcttga agagcctgag ccaaactcat accttgacca
    17701 aaggaaatac taccactgct atatgatgcc ttaaagcttg tatttgcagg acgagccaga
    17761 ccttgatact caagcaagtc tgaactgatt tcgttaataa aacgagtcgg acggttataa
    17821 ttggtacgac caaaaagcaa gcgtgagttg gcattggtca gatagagaat tttctctgca
    17881 cgcgtgatac ctacataggc tagacggcgc tcttcttcta attcatctgg atcttcagtc
    17941 gcacgactaa gtggaaagac attttcttcc atcccaatca aaaagacaac tggaaattcg
    18001 agacctttgg cagcatgcag ggtcatcaag gtcacttctg atgtctcctg actacctgaa
    18061 tctgtgtcgg caatcaaagc caagtcattt aagaaacgac tcagtttgtc cagaccagtt
    18121 tcctcttctg tcacatcagt ggtgtcatca aagttcttcg taacagaaag aaactcttcg
    18181 atattttcaa cccgtgcctt gctttctaga gtcgcttggg cgttaagaat atcgacataa
    18241 cctgtttttt ctaggacgga ctcaaccaac tctgtaatgt ttaagtggtc tagctgctcc
    18301 cgcaaatcaa gcatcatatt ggcaaaatcc cagatagatt gggctgcctt acccttgata
    18361 ccagacaaca taatattagc agaagcatcc agcatagaca tattttgcaa atttgcaaaa
    18421 tcacggattt tctcaactgt acctagacca attccacgtt taggctcgtt gataatacgc
    18481 tcaaaactaa tattgtcact caaattagca ataaggttga gataagcaat aatatcgcga
    18541 atttccttac ggctgtagaa tttggttccg ccaaccatgg tataaggaat gttagacttg
    18601 agcagggctt cctcaattgt acgggactga gcattagtcc gatagagaac tgcaaaatcc
    18661 ttatgaagga agttttgact gcgactaagt tcatcgatgg ttctggctac aaatacagcc
    18721 tcatccagct catcatcggc acgatagtaa acgatttgct ccccatcagc gttttgagtc
    18781 cagagatttt taggacggcg atttttatta tttttaataa cctcgttggc cgcttggaga
    18841 atggttttgg ttgagcggta attttcctcc aacaaaacaa ccttggcttt ggggtaatcc
    18901 ttttcaaagt ccaagatatt ctgcatatca gcaccacgcc aaccgtagat agactggtcc
    18961 gcatccccaa ccacacagat atttttaaaa cgggaagcca agagtttgac taattggtac
    19021 tgagcgtggt tggtatcttg gtactcatca acgtggatgt attggaattt ttgctggtag
    19081 taggtcaaaa catcaggatt ttgatcaaag agacgcaagg tcagcataat caaatcatca
    19141 aagtcaacgg attcagactg acgaagttct ttttgatagg ctgtataaca ctgggccaca
    19201 atttgcgtat acatatcgcc agcttgggca gcataagcaa catcatcaat caaatcattc
    19261 ttagcattgg aaatggtccc caaaatagtt cgttcattcc attttttagg atccaagttc
    19321 aactgtttga gaatacgttt catgagcgtt cgctgttcac caggatccac aattgtaaaa
    19381 ttacgattgt agccaatatg gtccgcatcg cgacgcaaaa tacgcacaca catggagtgg
    19441 aaggtcgcaa tcagacagtc ctgagtcgct ggattgaggc tataagcacg ctctttcatc
    19501 tcacgcgcag ccttgttggt aaaggtaatg gccaagatat tccaaggatt gaccagcttt
    19561 tcatcaatca aataagcgat acggtgggtc aaaacacgag tctttccaga accagcccct
    19621 gccatgatta gcaagggacc ttctgtcgtt tgcaccgcct cagcctgacg gtcattcatt
    19681 ccatttaata atgcgttcat cttctcttcc ttactcttga agtcaatatt tctattatat
    19741 cagaaatcag ggaaaataga aactttacgg ggagtagcaa aatatagtgg attgaaacta
    19801 gaatactaca ctgtgggttt ctaaaacact cgactttttc acccaatttt ttcataaact
    19861 agctcaatac ctca
//
LOCUS       DBERCA010000034        19779 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.034, whole genome shotgun sequence.
ACCESSION   DBERCA010000034 DBERCA010000000
VERSION     DBERCA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.034"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            371..1534
                     /locus_tag="ACL719_001337"
     CDS             371..1534
                     /locus_tag="ACL719_001337"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGS0699295.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGNYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLTMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1531..2208
                     /gene="aroD"
                     /locus_tag="ACL719_001338"
     CDS             1531..2208
                     /gene="aroD"
                     /locus_tag="ACL719_001338"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGS0699296.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2198..3052
                     /locus_tag="ACL719_001339"
     CDS             2198..3052
                     /locus_tag="ACL719_001339"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGS0699297.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3071..4138
                     /gene="aroB"
                     /locus_tag="ACL719_001340"
     CDS             3071..4138
                     /gene="aroB"
                     /locus_tag="ACL719_001340"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGS0699298.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4148..5314
                     /gene="aroC"
                     /locus_tag="ACL719_001341"
     CDS             4148..5314
                     /gene="aroC"
                     /locus_tag="ACL719_001341"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGS0699299.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5324..6427
                     /locus_tag="ACL719_001342"
     CDS             5324..6427
                     /locus_tag="ACL719_001342"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGS0699300.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6438..6776
                     /locus_tag="ACL719_001343"
     CDS             6438..6776
                     /locus_tag="ACL719_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGS0699301.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6868..8151
                     /gene="aroA"
                     /locus_tag="ACL719_001344"
     CDS             6868..8151
                     /gene="aroA"
                     /locus_tag="ACL719_001344"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGS0699302.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8144..8620
                     /locus_tag="ACL719_001345"
     CDS             8144..8620
                     /locus_tag="ACL719_001345"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGS0699303.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQRDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8617..9465
                     /gene="pheA"
                     /locus_tag="ACL719_001346"
     CDS             8617..9465
                     /gene="pheA"
                     /locus_tag="ACL719_001346"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS0699304.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9462..10736
                     /gene="psr"
                     /locus_tag="ACL719_001347"
     CDS             9462..10736
                     /gene="psr"
                     /locus_tag="ACL719_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGS0699305.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11015..11860
                     /locus_tag="ACL719_001348"
     CDS             11015..11860
                     /locus_tag="ACL719_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0699306.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVVFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11850..12953
                     /locus_tag="ACL719_001349"
     CDS             11850..12953
                     /locus_tag="ACL719_001349"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0699307.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12955..13941
                     /locus_tag="ACL719_001350"
     CDS             12955..13941
                     /locus_tag="ACL719_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0699308.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13957..15390
                     /locus_tag="ACL719_001351"
     CDS             13957..15390
                     /locus_tag="ACL719_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGS0699309.1"
                     /translation="MIDGKRLLFSLTIVSYTLTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15419..16453
                     /locus_tag="ACL719_001352"
     CDS             15419..16453
                     /locus_tag="ACL719_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS0699310.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16595..17332
                     /gene="mecA"
                     /locus_tag="ACL719_001353"
     CDS             16595..17332
                     /gene="mecA"
                     /locus_tag="ACL719_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGS0699311.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFAEDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17482..18768
                     /locus_tag="ACL719_001354"
     CDS             17482..18768
                     /locus_tag="ACL719_001354"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGS0699312.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18770..19639
                     /gene="thrB"
                     /locus_tag="ACL719_001355"
     CDS             18770..19639
                     /gene="thrB"
                     /locus_tag="ACL719_001355"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGS0699313.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGSTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
BASE COUNT         5889 a         3590 c         4504 g         5796 t
ORIGIN      
        1 gtatattgag tttgtaacaa aaggcttctc tataaaatca aattttccag atatctactg
       61 aagctgattt atttcttcat ctttcttcta ctacctttgt cactaaattt gacagacaca
      121 tattgctgat acaacctctc tacctataat ctcacagtta acctgaggct agtttcatag
      181 tttgctattt gattttcgtt gataaaaaaa atgctcctcc aagacggaag agcatttttt
      241 attgtgattc ttacacattt acttcctagt tttgcaggaa gtctagtata ccatttttta
      301 aaagaagagt aaaaaagcaa gggtagtttc ttttttgaga attgtctaaa agtttgatat
      361 aatggtagtt atgaatagaa taagagtcag caaaagggtt gaaaagaagc ttgctaaggg
      421 gctagtttta ctagaagcca gtgatcttga gaatgtcaat cttaaggatc aggaagtaga
      481 ggtgcagggt caggaaggaa actttcttgg gactgcctac ctttctcagc aaaacaaggg
      541 cttgggctgg tttgtcagca aagacaaggt ggccttcaat caagctttct ttgaaacgtt
      601 gtttagaaaa gccaaagaaa agagaagcgc ctactatcaa gatgatttaa caactaccta
      661 tcgtctcttt aatcaagagg gagatggctt tgggggtctg acagtggacc tttatggcaa
      721 ctacgccgtc ttttcttggt ataactctta tgtttatcag attcgtcaga ctatatcaga
      781 agcctttaga caggttttcc ctgaggtttt aggggcttat gagaaaatcc gctttaaagg
      841 tttggactat gaatctgccc atgtttatgg tcaagaagca cctgactttt tcactgtttt
      901 agaaaatggt gtcctgtatc aagtctttat gaatgatggc ttgatgacag gaattttcct
      961 agaccagcat gaggttcgcg gtagtttagt tgacggcttg actatgggta aatccttgct
     1021 caatatgttt tcctacacag cggctttttc agtagctgcg gccatgggag gagctagcca
     1081 gacaacttct gttgatctag ccaaacgttc acgagaattg tctcaagcgc attttcaggc
     1141 aaatgggctc agcacagacg agcatcgttt tatagtcatg gatgtctttg agtatttcaa
     1201 atatgccaaa cgcaaagact tgacctacga tgtgattgtc ctagatccgc ctagctttgc
     1261 tcggaataaa aaacaaactt tctctgtggc caaggattat cacaagttga tttcccagag
     1321 tcttgagatt ttaaatccgg gagggattat cattgccagt accaatgctg ccaatgtttc
     1381 ccgtcagaaa tttacagaac aaattgataa aggctttgca ggaagaagtt accagatttt
     1441 aaacaaatat ggtcttccag cagattttgc ctataataaa aaagatgaaa gtagtaatta
     1501 cctcaaggtg attagtatga aggttagtaa atgaaattaa tcgtttcagt aatgccaaga
     1561 agtttagagg aggctcaggc tctggatgcc acgaggtacc tggatgccga catcattgaa
     1621 tggcgtgccg actatctgcc taaagaagcg attttgcagg tagctccagc catttttgaa
     1681 aaattcgcag gccgtgagtt ggttttcacg ctacgaactc gctccgaagg tggagaaatc
     1741 gacctttctc cagaagagta tatccatcta atcaaggaag ttgcgcaatt ctatcaacca
     1801 gactatattg attttgagta ctatagctac aaggatgttt ttgaggaaat gctggacttc
     1861 ccaaatcttg ttttgagtta ccataatttc caagaaacac ctgagaacat gatggaaatc
     1921 ttgtcagagt tgacgatcct aaatccaaaa cttgttaagg ttgcggtgat ggctcacacg
     1981 gagcaggatg tcttagactt gatgaactat acacgaggct ttaaaaccct caatcctgaa
     2041 caggaatatg tgaccatttc tatgggtaag gtgggcaagg tctctcgtat cactgcggat
     2101 gtgactggtt cgagttggtc ttttgccagt ctggatgagg ttagtgcccc tgggcagatt
     2161 tctctagcta gcatgaaaaa aatcagggag attttggatg aagcttgatg gctatacacg
     2221 tttagctgca gttgttgcca atcctattaa gcattctatt tctcccttca tccacaatag
     2281 agcctttgag gcgacagcta ccaacggtgc ttatgtggct tgggagattg aagcgagtga
     2341 cttggcagaa acagtggcca atattcgtcg ctaccagatg tttggcatca atctgtccat
     2401 gccctataag gagcaggtga ttccttattt ggataagctg agcgatgaag cgcgcttgat
     2461 tggtgcggtt aatacggttg tcaatgagaa tggcaattta attggatata atacagatgg
     2521 caagggattt tttaagtgct tgccttcttt tacaatttca ggtaaaaaga tgaccctgct
     2581 gggtgcaggt ggtgcggcta aatcaatctt ggcacaggct attttggatg gcgtcagtca
     2641 gatttcggtc tttgttcgtt ccgtttctat ggaaaaaaca agaccttacc tagacaagtt
     2701 acaggagcag acaggtttta aagtggattt gtgtgcttta gagtatgttt ctgaactgca
     2761 agcaaggatt gccgagtcgg atttgctagt taatgccacc agtgtgggca tggatggcca
     2821 atcctctcct gttcctgaaa acatagtctt accagaaact ctcttagttg cagatatcat
     2881 ataccaaccc tttgaaacac cgtttttgaa atgggctaga agtcagggca atccagcagt
     2941 caatggtctg ggaatgttgc tatatcaagc tgcagaagct tttcaactgt ggacaggcaa
     3001 ggaaatgccg acagaagaga tttggcagtc tttaacagaa aaataccaat aaagaaaggg
     3061 agactctcct atgaaaatca gaatcgatat tcctcatcat ccttatgata ttcagattga
     3121 aaaaggttgt atggcccagg ctggtcagtg gttgcgagaa ctctggcaac cgcaaaaggt
     3181 agtcattgtg acagataacc atgtagcctc tctctatgca gagaaggtca agctcagcct
     3241 agaagatgct ggttttcagg tagctgtttt tgatttctta gaaggtgaag aaagaaagaa
     3301 tttaactact gttcagaaag tctatgaatt tctagtcaag caaggtctga ctcgtagcga
     3361 tggaatcgtt gctcttggtg gtggcgttgt tggggacctg gctggatttg tagcctctac
     3421 ctatatgcgg ggtattcact ttgttcagat tccgactagt ttgacagctc aggttgattc
     3481 ttctatcggt ggaaagacag gcgttaacac accatttgct aaaaatatgg tggggacctt
     3541 tgcccaacca gatggggttc tgattgatcc acttgttctt gaaaccctcg gaaaaagaga
     3601 gttgattgaa gggatgggtg aagttatcaa gtatggcttg attgaggatc cagaactgtg
     3661 ggctctcttg acggggctga atggttctgt tgagagcatt ttggaacatg cagagacctt
     3721 gattgaacat tcttgtcagg tgaagcgcaa gatggtggtt gaagatgagt tggacaatgg
     3781 tattcgtctt tacctcaatt ttggccacac tattggccat gccatcgaag cgactgccgg
     3841 ttatggcaag gtcatgcatg gagaggctgt tgccatggga atggtacaga tttccaagat
     3901 tgctgaggaa aaaggcctca tgccagctgg cattacccaa tctatcacag agatgtgtca
     3961 gaaattcggc ttgcctgttg actatgaaaa ttgggaagtt gacaagcttt atcaggctct
     4021 tactcatgac aagaaagcgc gtggtaacac cttgaaattg gtcttggtgc cagagcttgg
     4081 ttcagcgacc attcatccag tttctctgga agagatgaaa gactacttgg taaaataagg
     4141 agagtgtatg agatatttaa ctgcaggaga atcacacggc ccccgtctaa cagctattat
     4201 tgagggaatt ccagctggac ttccattgac agctgaggat atcaatgagg accttagacg
     4261 ccgtcagggt ggctacggtc gtggtggtcg tatgaagatt gagagtgacc aggttgtctt
     4321 tacttcgggc gttcgccacg ggaagacgac aggggcgcct attactatgg atgtcatcaa
     4381 taaggaccac cagaaatggc tggacatcat gtctgcggag gacattgaag accgccttaa
     4441 aagcaagcgg aaaattactc atcctcgccc aggtcatgcc gatttggttg gggggattaa
     4501 gtaccgtttt gatgatttgc gaaattcttt ggagcgttca tcagctcgtg aaaccaccat
     4561 gcgggtggca gttggtgcag tagccaaacg tctcttggct gagctggata tggagattgc
     4621 caaccatgtc gtggtctttg gtggcaagga aatcgatgtt cctgaaaatc tgacagtcgc
     4681 tgaaattaag caaagagctg cccagtctga agtttctatt gtcaaccaag aacgagaaca
     4741 ggaaatcaag gactatattg accaaatcaa acgtgatggt gataccatcg gtggggttgt
     4801 ggagacagtc gtcggaggcg ttccagttgg tcttggttcc tatgtccaat gggatagaaa
     4861 attggatgca agattggctc aagctgttgt ctctatcaat gcctttaaag gggtggaatt
     4921 tggtcttggc tttgaggctg gttatcgtaa aggcagccaa gttatggatg aaattctctg
     4981 gtctaaagaa gacggttata ctcgccgtac caataatcta ggtggttttg aaggtggtat
     5041 gactaatggg caacccatcg ttgttcgtgg ggttatgaaa cccattccta ctctttataa
     5101 acctcttatg agtgtggata tcgaaaccca cgaaccttac aaggcaaccg tggagagaag
     5161 tgatccgact gctcttccag ctgcaggaat ggtcatggaa gcagttgtag caacggttct
     5221 ggcgcaagaa atcctcgaaa aattctcatc agataatctt gaggaactaa aagaagcggt
     5281 agccaaacac cgagactata caaagaacta ttaaggagtt cctatggcaa aaacaatcta
     5341 tatcgcaggt cttgggttga ttggagcctc tatggcactt ggtatcaaac gcgatcatcc
     5401 agattatgaa attttaggtt ataatcgtag tcaagcttcg agagacatcg ccttgaaaga
     5461 aggcatgatt gaccgtgcaa cggatgattt tgctagtttt gctcctttgg cagatgtcat
     5521 tatcctcagc ttgccaatca aacaaactat tgctttcatt aaggagttgg ccaatttgga
     5581 tttgcgagaa ggcgttatta tttcagatgc tggttcgacc aagtcaacca ttgtggatgc
     5641 ggcggagcag tatttggctg gcaagtctgt tcgctttgtc ggggcccatc ccatggctgg
     5701 tagtcacaag acaggggctg cttcggcaga tgtcaatctt tttgaaaatg cctattatat
     5761 ctttacacct tcaagcctga caagtcagga cacgcttaag gaaatgaagg atctgctttc
     5821 aggtcttcat gctcgtttta tcgagattga tgccaaggag catgatcgtg tcacttctca
     5881 gattagccat tttcctcata ttttggcttc tagtctcatg gagcagactg cggtctatgc
     5941 tcaagagcat gagatggcaa ggcgctttgc ggcaggtggt tttcgagata tgacccgaat
     6001 tgcggaaagc gagccaggaa tgtggacctc cattctcttg tccaatagcg agaccattct
     6061 ggatagaatt gaggatttca aggaacgttt ggaagcgatt ggtcaggcca ttagtaaggg
     6121 agatgaagag caaatttgga acttttttaa ccaagcgcgt gaacaacgcc agacaatgga
     6181 aatccataag cgtggtggtg tggatagctc ttacgacctc tatgttgacg ttcctgatga
     6241 agaagatgtc atcctgcgga ttttggaact gctacgtggg acttccttgg ttaacatcca
     6301 catcaacgag gaaaatcgtg aggatattca cggaatccta caaatttcat ttaaaaatgc
     6361 tcaagacttg gaaagagccg agcatctcat aacaaaaaat accgactata cagtcgtcat
     6421 taaataagga gaagaacatg tcaaatattt acgatagtgc aaacgaactc agtcgcggtc
     6481 tacgcggatt accagaatac aaggctgtta aagcagctaa agatgcgatt gcagcagatg
     6541 ctgaggcaag caaaatcttt acagaatatc ttgcctttca agaggaaatt caaaaactag
     6601 cacagacagg tcaaatgcca gatgcgtcct tccaagctaa gatggaaggc tttggtaaac
     6661 agattcaagg aaatagcctc ttgtcagaat tctttaccaa gcaacaacaa ttggccattt
     6721 acctttctga cattgaaaaa attgttttcg aaccagtttc agaattgctg aaataagaaa
     6781 agaactatcc taaagtcagg aatttttaag gaatttctgg ctttttatga taaaataggt
     6841 aaaagtattg caagtatgag gtccagtatg aaactaaaaa caaacattcg ccatttacat
     6901 ggtagtatcc gcgtcccagg tgacaagtct atcagccacc gttccattat ctttggaagt
     6961 ttggctgagg gtgagaccaa ggtttatgat attctgcgag gtgaagacgt tctttcgacc
     7021 atgcaggttt ttcgtgacct tggtgttgaa attgaggata aagatggggt tattaccgtt
     7081 caaggtgtag gcatggctgg cttaaaagcg ccgcaaaatg cccttaatat gggaaattct
     7141 ggcacctcga ttcgcctgat ttcaggtgtc cttgctggtg cagatttcga agtagagatg
     7201 tttggagatg atagtctttc caaacgtcct atggaccgtg tgacccttcc actgaaaaaa
     7261 atgggcgtca gcatctcagg gcaaactgaa cgagacttgc ctccccttcg cttaaaaggg
     7321 acgaaaaacc taagacctat tcattatgag ttgccaattg cctctgccca agtcaagtca
     7381 gccttgatgt ttgcagcctt acaagctaag ggggagtcag ttattatcga aaaagagtac
     7441 acccgtaatc atactgaaga tatgttgcaa caatttggtg gtcatttaag tgtggatggt
     7501 aagaaaatca cagtccaagg gccacaaaaa ttgacgggac agaaggtggt cgtaccagga
     7561 gatatttcca gtgcagcctt ttggttagtc gcaggtttga ttgctccaaa ttctcgtcta
     7621 gtgctgcaga atgtggggat aaacgaaact cgcaccggta ttattgatgt cattcgtgcc
     7681 atgggtggaa aattggaaat aactgaaatc gatccagtcg ctaaatctgc aaccttgatt
     7741 gttgagtctt ctgacttgaa aggaacagag atttgtggcg ctttgattcc acgtttgatt
     7801 gatgaattgc ctattattgc cctacttgcg acccaagccc aaggtgtaac agttatcaag
     7861 gatgctgagg agctcaaggt caaggaaaca gaccgtattc aggttgtggc agacgcctta
     7921 aatagtatgg gagcagatat tactcctacg gcagatggga tgattatcaa aggaaaatca
     7981 gctcttcacg gtgctagagt caatacgttt ggtgaccacc gtatcggcat gatgacagct
     8041 atcgcagccc tattggttgc agatggagag gtggagcttg accgtgcaga agccatcaat
     8101 accagctatc ctagtttctt tgatgatttg gagagcttga ttcatggcta aggtgttatt
     8161 agggtttatg ggggctggaa aatcgactat tgcaagaggc ttggacccta attaccttga
     8221 tatggatgct ctgattgaga agcgcctagg tatgtccatt gcgaattttt tcgctgaaaa
     8281 gggagaagca gcctttcgtc aggtagaatc agaagtccta gctgatttat tacaaagaga
     8341 ccaagtcgtg tcaactggag gaggagtggt tatttctcag agaaatcgtg acttactcaa
     8401 gactaataca gataacatct acctgaaagc agattttgaa accctctacc tacgtatcgc
     8461 agctgataag gacaatcagc gaccgctttt tctaaataat agcaaggaag aactagtagc
     8521 tatttttcaa gaaagacagg cttggtatga ggaagtggct agtcgggttt tggatgtgac
     8581 caagctaagc ccagaggaaa ttatagagga actaagatga aaattgctta tctaggtccc
     8641 aaaggatcat tttcacacca cgttgtgcag acagcttttc ctcatgagga attgcaggct
     8701 tttgccaaca ttacagatgt catcaaggct tatgagcaag gattagtgga ctattctgtg
     8761 gtgccagttg aaaattctat tgagggtagt gttcatgaaa ccttggacta tctttttcat
     8821 caggctcaca tccaagcagt agcagaaatc gttcagccta ttcatcagca gttgatggtg
     8881 gttccaggcc atactaagat tgaaaagatt ttttcacatc cacaggcctt ggctcaagga
     8941 aagaaattca tcgatgaaca atatccagag gctcaaatcg aggtaacagc tagtacagct
     9001 tatgcggccc gttttatttc cgaacatcca gatcagccct ttgcagcaat tgcacctaga
     9061 agttctgctg aagaatatgg attggaactg attgccgagg atattcagga aatggaagcc
     9121 aatttcacac gtttctggct tctaggagct gaaaagccta gtattccctt gcaagcacaa
     9181 actgaaaaga tgagtttggc cttgacatta cctgacaacc ttccaggtgc actttataag
     9241 gccctgtcga cctttgcttg gcgaggaatt gacttgacaa aaattgaaag tcgtccactc
     9301 aagacagcac tgggtgaata ctttttcatt atcgatgtgg attataccga taaggacttg
     9361 gtccactttg cccaaaaaga attagaagcg attggaatcc agtataaaat tctgggtgcc
     9421 tatcctattt atccaatatc agaccatgga aaggagagaa gatgagtaaa gaaaatccct
     9481 taagtcatca tgagcagttg cgttatgatt atttgctaaa aaatattcac tatctcaatg
     9541 agagagaaaa aaatgagttt gtctatttgc aagaaaagct aactcttgct aggggaaata
     9601 gtagctctag cttggaacaa gaaagagaag agcaggttga cttaccaagc tatgcgaacc
     9661 ggagtcgctc acaatccaaa tcacaagcac tctctttccc tccaaaaaag aaaagacgga
     9721 agctccgtct taaacgaatt tttatggtga ttttttcact tttggtctgt gtggctttgg
     9781 ccatggtatt catgtttttg cgtggttacc aagatgctag cgcaaagaaa actgctgatg
     9841 cccgggcagc tcaagtagaa gtctttaatg gtcaggacac tagagatgga gttaatattt
     9901 taatcatggg tactgatggt cgaatcggcc agaacagtgt tgagacacga actgactcta
     9961 ttatggtatt aaatgtcggg ggctcagata agaaaatgaa gctagtcagt ttcatgcgtg
    10021 ataatttggt ctatatagat ggttatagtc aagtgattaa cggtagaaaa cagacagata
    10081 acaagttaaa cgtagcctac gagttaggag aacaagaagg gcaaaaaggg gcagaaatgg
    10141 ttcgccaagt cttgaaagat aattttgact tggacattaa gtactacgcc ttggttgatt
    10201 tccaggcctt tgccacagcg attgacacgc ttttccctga tggggtgacg attgatgctc
    10261 aattttcaac attgaatggg cgtcccctaa cagaagctac agtcggagat gatttatacg
    10321 ctaccgagac tgagtctcca acccaaacca ttaaagtcgg aaaacagcag atgaatggct
    10381 cgaccctgct caattatgct cgtttccgtg atgatgatga ggcggattac ggccgtacaa
    10441 aaagacagca acaagtttta acagcaattt tagagcaaat taaagatccc actaaacttt
    10501 tcactggttc tgaagctctt ggaaaggttt ttgctatgac ctcaaccaac gtaccctata
    10561 ctttcctcct aacaaacggt ttatctgttt tggatggagc aaaaaatggt attgaaaaat
    10621 tgacgattcc agaactgggt gactgggtag atgcctatga tgtttatgga ggcttgggct
    10681 tgctggttga tcaaaacaaa tatcaaacca agctcgctca aatgggcttg agataagata
    10741 atactcaatg aaaatcaaag tgcaaattag gaagctagcc gcaggctgct aaaaacacag
    10801 ttttgaggtt gtagataaga ctgacgaagt cagttaccta tacctacggc aaggtgaagc
    10861 tgacgttgtt tgaagagatt ttcgaagagt ataagacaga aaaatcaaag tttgatagct
    10921 atttggatag cagtcaggct ttgatttttt acagtctgca atagattttc accccttgtt
    10981 tgtggtataa tgaaacgata cgatagaaag aataatgaac aatatgactg atttaaaagc
    11041 aattcaggct cgtagtctgg agatggctga atattttgtg gtcttttgta aagaacatga
    11101 tttgctctgt tatctctgtg gcggaggcgc tattggtgcc cttcgaaaca agggatttat
    11161 tccttgggac gacgacctag acttttttat gcctcgtaaa gattatgaga aattagcaga
    11221 attatggcct cgttatgcag atgaacgtta tttcttgtca aagagtcaca agaattttgt
    11281 tgatcgtaat ctttttatta ccattcgtga caaggaaacc acctgtatca agccttatca
    11341 gcaggatttg gatttgccac atggtctggc cttggatgtt ttgcctttgg attattatcc
    11401 gaaaaatcca gctgagcgga aaaaacaggt tcgttgggcc ttgatttatt cactcttttg
    11461 tgcgcaaact attccagaaa agcatggtgc tctcatgaaa tggggaagtc gcattttact
    11521 gggtttgact ccaaaatctc tccgttatcg catctggaaa aaagctgaga aagaaatgac
    11581 taagtatgat ttggctgatt gtgatggcat tacagaatta tgctcaggtc ctggctacat
    11641 gagaaacaag tacccaatca catcttttga agacaatctt ttcttgccat ttgaaggaac
    11701 agagatgcct attccaatcg gctatgatgt ctatctcaga actgcttttg gggattatat
    11761 gacgcctcca ccagcagaca agcaggtacc gcatcatgat gctgtcatcg ctgatatgga
    11821 taagtcttat acagaataca agggagaata tggtggctaa gaaaaaaatc ttatttttta
    11881 tgtggtcttt ttctcttgga ggtggtgcag agaagattct atcaaccatt gtttcaaatc
    11941 tggatccaga aaagtatgat attgatattc ttgaaatgga gcactttgac aagggatatg
    12001 aatctgttcc aaagcatgta cgcattttaa aatcccttca agattatcgc caaaccagat
    12061 ggttacgagc ttttttgtgg agaatgagaa tttattttcc aagactgact cgtcgtttgc
    12121 ttgtaaaaga tgattatgat gttgaagttt cttttaccat tatgaatcca ccactgttgt
    12181 tctctaaaag aagagaagtc aagaagatat cttggattca tggaagtatt gaagaacttc
    12241 ttaaggatag ctctaaaaga gaatcacata gaagccagtt ggatgctgcg aatacaattg
    12301 tagggatttc aaaaaagacc agcaattcta tcaaggaagt ttatccagat tatgcttcta
    12361 aattacagac aatctacaat ggatatgatt ttcagactat tctagaaaaa tctcaagaga
    12421 agatcgatat cgagattgct cctcaaagta tctgtactat cggacggatt gaggaaaata
    12481 agggttctga ccgtgtagtg gaagtgatac gattattaca ccaagaggga aaaaactatc
    12541 atctctattt tatcggggct ggtgatatgg aagaggaact gaaaaaacga gtcaaagagt
    12601 atgagattga ggactatgta catttccttg gttatcaaaa aaatccttat cagtatttat
    12661 ctcagacgaa agttctcttg tctatgtcta aacaagaagg ctttcctgga gtgtatgtgg
    12721 aggccttgag tctgggactc ccttttatct ctacggacgt tggaggggct gaggaattat
    12781 cccaagaagg acgatttgga caaatcattg agagcaatca agaggcagct caggcgatta
    12841 ctaattacat gacttctgcc tcaaacttta atgtcgatga ggctagccaa ttcattcaac
    12901 aatttacaat tacaaaacaa atcgaacaag tagaaaaact attagaggag tagcatggaa
    12961 actgcattaa ttagtgtgat tgtgccagtc tataatgtgg cgcagtacct agaaaaatcg
    13021 atagcttcca ttcagaagca gacctatcaa aatctggaaa ttattcttgt tgatgatggt
    13081 gcaacagatg aaagtggtcg cttgtgtgat tcaatcgctg aacaagatga cagggtgtca
    13141 gtgcttcata aaaagaacga aggattgtcg caagcacgaa atgatgggat gaagcaggct
    13201 cacggggatt atctgatttt tattgactca gatgattata tccatccaga aatgattcag
    13261 agcttatatg agcaattagt tcaagaagat gcggatgttt caagctgtgg tgtcatgaat
    13321 gtctatgcta atgatgaaag cccacagtca gccaatcagg atgactattt tgtctgtgat
    13381 tctcaaacat ttctaaagga atacctcata ggtgaaaaaa tacctgggac gatttgcaat
    13441 aagctaatca agagacaaat tgcaactgcc ctatcctttc ctaaggggtt gatttacgaa
    13501 gatgcctatt accattttga tttaatcaag ttggccaaga agtatgtggt taatactaaa
    13561 ccctattatt actatttcca tagaggggat agtattacga ccaaacccta tgcaaagaag
    13621 gatttagcct atattgatat ctaccaaaag ttttataatg aagttgtgaa aaactatcct
    13681 gacttgaaag aggtcgcttt tttcagattg gcctatgctc acttctttat tctggataag
    13741 atgttgctag atgatcagta taaacagttt gaagcctatt ctcagattca tcgtttttta
    13801 aaaggctatg cttttgctat ttctaggaat ccaattttcc gtaaggggag aagaattagt
    13861 gctttggccc tattcataaa tatttcctta taccgattct tattactgaa aaatattgaa
    13921 aaatctaaaa aattacatta gaacgggggt gaccaagtga ttgatgggaa acgattatta
    13981 tttagtttga ccatagtcag ttataccttg acgctagtaa gtggaattgt gtatctgttt
    14041 aataataata atgttagctt actttctact ttattgttct tgttggttag tagcttaatt
    14101 gcttgttgga atgatatcaa gtattactta atccatttta ttttctattt aaccattttt
    14161 gtatttctgg tatcaagacc gaccattgat tattttaggg atggtgcttt ggatacctat
    14221 catccaatag cctatcgttt tgcctttata gttgtcatgg tttcgattct gggcttgacc
    14281 acaggaggcg tcctggctcg ttacttcata gctaggaaga aaataaaagt agcaaatata
    14341 ggaaattctc taaaagaggt ttatatcaag cggttacgct ttgtatcact aggagttttt
    14401 cttctaactt atcctttcta tttcattcgg ttatttgaac ggctcttgta tcgtttgcag
    14461 acttcctact atgcctacta tgcaaatttt gaaagtaaac tgccttattt tacctacatt
    14521 ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg caaccaagcc aaagaaattg
    14581 caggccacag cagtgcttgt ctcctttatt gcagctaata ctattcattt ggcaattggg
    14641 acacgaaatc cctttatttt aagtatttta tttgcttttg tttattactt tatgcgggag
    14701 caaactgaaa aaggaaaatg gattgggttt aaagaaaagt tagcgatttt tgtaggttct
    14761 cctattctca tgttagcgat gggagtactc aattatgtac gggataatgt ccaagtttcc
    14821 catacaggtt tctgggatat cttacttgac tttatctata aacaagggac tagttttggt
    14881 gttctggctc gaggttttct atttaacagt agcctccctt accgagattt ccgtaatttt
    14941 acttttggtc ctgttcttga ttattttgca agggggagtt tgggagccat tttcggagga
    15001 aaagcctttg aacatacaac caatagtgtg gaactagcta ttgatagtaa tagttatgcc
    15061 cacaatctat cctatcttgt cttgaacaag gaatacttga aagggcatgg tatcggaagt
    15121 agttatatca tggagttgta taccgactat ggtatgattg gagtctttct gcttagtttc
    15181 ttactcggcg tattatttat agccatgctg caagtagcct atcgctcaag gacaatccta
    15241 tttgctttat ccctactcat cttgaataat ctattcttta tgccaagaag cagcttttca
    15301 gaaagtttct tcaatttatt tacaatgcaa ttctggggaa ttgttcttgt gattatattt
    15361 gtagcaaaaa tgcttacaaa ggaaaactag tatctactaa acaaaggaga aaaaaatcat
    15421 gtttgaacat tattcagtag ctgatttgtt tgcaaatctt tataaaaagc gaaaagcaaa
    15481 tattttagct ctcatcgctt tatttgctct cattgctgta ccatttacaa ttaaagcagt
    15541 taggaataaa aatactgtca aagatacaac aagttattca acttatctta tctataaaat
    15601 cactcctcca aaagagtcgg ccaaaacgat tttgaatcat caaattggtg gttatagtga
    15661 tttttatggg aaattgattg atggtaattt gaatggagct tatcttttca atgatgtaga
    15721 acccagtgag ttgaaaaaaa ttgccagtga attagacacg acagaaacaa ccttgaaaaa
    15781 ttcaacgaat gactattggt ggaaaaaatt gaccgtctac tatatgattg acgatgcagg
    15841 ggttggtgtg aaaattttga catcaagtaa agatgccaat gacttgttag agaaaaaaat
    15901 tgatgggttg attgagaaat ttaaacatgc ttatgcaaat gtgaaaattg aaaaactgga
    15961 aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca gcgcttggct taaatgtgaa
    16021 aaatctgatt cttcgtttag ttgttattgg agtggtttgt gtgattttgg ttgtgatggg
    16081 aaatgtgtta gtttatctct ttaatccaac aatcaataga gtaggtgatt tttctcagta
    16141 tcaaattgat tttgtaacag agattacaac aattgctaac ctagcagatg ttttgtcata
    16201 caaaaatact ggacaggaat tgaccatcgt tagctcaaat aaagctatcc tagataaatt
    16261 gaaacagagt caagaagctt taaaaggaat gcattttgta gatttacagg atgtatcatc
    16321 tcttttggaa agagatacag tccttcttgt tgaagagtac ggagtgactc gttataagaa
    16381 atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt tctatcctcg gtgtagcaac
    16441 ctttaaatta taagtattct gatgtataag gtcttcaaag tcggctaaga gccgattttg
    16501 aaggcttttt ctatctgtct gcagaaattt tcttttgata aaatcgtaaa aatctagtat
    16561 aatagataga actgaaagta tgaggttact agcaatgaaa atgaaacaaa ttagtgatac
    16621 aactttaaaa atcacgatgt ctttagagga tttgatggat cgtggaatgg agattgctga
    16681 ctttctcgtt cctcaagaaa aaacagaaga gttcttttat gctatcttgg atgagctaga
    16741 gatgcctgat agctttctgg atacaggcat gttgagcttc cgtgtgactc caaaacctga
    16801 taaggtagat gtctttgtga ccaagtcaaa gattgaccaa aatctagatt ttgaagactt
    16861 atcggatttg ccagatatgg aagaattggc tcaaatgtct ccagatgaat ttatcaaaac
    16921 cctggaaaaa agcatcgcag acaaaaccaa ggatgatatc gaagccattc aatctcttga
    16981 gcaagttgaa gccaaggaag aagagcaaga gcaggctgaa caagaagctg agagtaagaa
    17041 agaaccttac atctactaca tcctttcttt tgctaagttg gctgacttgg tagcttttgc
    17101 caagacagtg acttttgaaa tggaaacttc tgaactctac aaaatgaacg agcgctatta
    17161 tttgaccatt ttagtggata ttgaaaatca tccaagccca tatccagctt ggctgttggc
    17221 ccgtatgcgc gagtttgcag aggatagtga tatcagtcgc tcagtcttac aagagtatgg
    17281 tcaagtcttg atgagtcacg atgcagtgct caatctgcaa aaaatcggct aatcctttct
    17341 ggaaagctat ttcagatttt aagaagagcg aatcgtctga ttcgtttttt ctttttatac
    17401 tgaaatagtg atttactata ataggaattt tcacaaaatt ctgttataat ggctatatta
    17461 gaaaatttcg aggagataaa gatgacagtt aaaattgctt tactaggatt tggtaccgtt
    17521 gcaagtggtg tgcctttcct cctaaaggaa aatggaggaa aaatcaatca atcagcacat
    17581 tcagatatca aagttgctaa ggtattggtc aaggatgaag atgaaaaaaa tcgcttgctt
    17641 gcagcaggga atgactttaa ctttgtaacc aatgtggatg atattttatc agaccaggat
    17701 attactatcg tagtggaatt gatggggcgt attgagcctg ctaaaacctt tatcactcgt
    17761 gccttggaag ctggaaaaca cgttgttact gctaacaagg accttttagc tgtccatggc
    17821 gcagaattgc tagaaatcgc tcaagctaac aaggtagcac tttactacga agcagcagtt
    17881 gctggtggga ttccaattct tcgtacttta gcaaattcct tggcttctga taaaattacg
    17941 cgcgtgcttg gagtagtcaa cggaacttcc aacttcatgg tgaccaagat ggtggaagaa
    18001 ggctggtctt acgatgatgc tcttgcggaa gcacaacgtc taggatttgc agaaagcgat
    18061 ccgacgaatg acgtagatgg gattgatgca gcctacaaga tggttatttt gagccaattt
    18121 gcctttggca tgaagattgc ctttgatgat gtagcccaca agggaatccg caatatcaca
    18181 ccagaagacg tagctgtagc tcaagagctt ggttacgtag tgaaattggt tggttctatt
    18241 gaggaaactt cttcaggtat tgctgcagaa gtgactccaa ccttcctacc taaagcgcac
    18301 ccacttgcta gtgtgaatgg cgtaatgaac gctgtctttg tagaatctat cggtattggt
    18361 gagtctatgt actacggacc aggtgcgggt caaaaaccaa ctgcaacaag tgttgtagct
    18421 gatattgtcc gtatcgttcg tcgtttgaat gatggtacta ttggcaaaga cttcaacgaa
    18481 tatagccgtg acttggtctt ggcaaatcct gaagatgtca aagcaaacta ctatttctca
    18541 atcttggctc tagactcaaa aggtcaggtc ttgaagttgg ctgaaatctt caatgctcaa
    18601 gatatttcct ttaagcaaat ccttcaagat ggcaaagagg gtgacaaggc gcgtgtcgtt
    18661 atcatcacac acaagattaa taaagcccag cttgaaaatg tctcagctga attgaagaag
    18721 gtttcagaat tcgacctctt gaataccttc aaggtgctag gagaataaga tgaagattat
    18781 tgtacctgca accagtgcca atatcgggcc aggttttgac tcggtcggtg tagctgtaac
    18841 caagtatctt caaattgagg tctgcgaaga acgagatgag tggctgattg aacaccagat
    18901 tggcaaatgg attccacatg atgagcgtaa tctcttgctc aaaatcgctt tgcaaattgt
    18961 accagacttg caaccaagac gcttgaaaat gaccagtgat gtccctttgg cgcgcggttt
    19021 gggttcttcc agctcggtta tcgttgctgg gattgaacta gccaaccaac tgggtcaact
    19081 caacttatca gaccatgaaa aattgcagtt agcgaccaag attgaagggc atcctgacaa
    19141 tgtggctcca gccatttatg gtaatctcgt tattgcaagt tctgttgaag ggcaagtctc
    19201 tgctatcgta gcagactttc cagagtgtga ttttctagct tacattccaa actatgaatt
    19261 acgtactcgc gacagccgta gtgtcttgcc taaaaaattg tcttataagg aagctgttgc
    19321 tgcaagttct atcgccaatg tagcggttgc tgccttgttg gcaggagaca tggtgaccgc
    19381 tgggcaagca atcgagggag acctcttcca tgagcgctat cgtcaggact tggtaagaga
    19441 atttgcgatg attaagcaag tgaccaaaga aaatggggcc tatgcaacct acctttctgg
    19501 tgctgggtcg acagttatgg ttctggcttc tcatgacaag atgccaacaa ttaaggcaga
    19561 attggaaaag caacctttca aaggaaaact gcatgacttg agagttgata cccaaggtgt
    19621 ccgtgtagaa gcaaaataaa gaatagaaga taggatgggg aaactcttga ccagagggtt
    19681 tcatatcctt tttgtgaaaa gaagtttata ctcaatgaaa atcaaagagc aaactaggaa
    19741 gctagccgca ggctgctcaa aacagtgttt tgaggttgt
//
LOCUS       DBERCA010000035        19203 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.035, whole genome shotgun sequence.
ACCESSION   DBERCA010000035 DBERCA010000000
VERSION     DBERCA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.035"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..498)
                     /locus_tag="ACL719_001356"
     CDS             complement(<1..498)
                     /locus_tag="ACL719_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HGS0699314.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
     gene            complement(535..663)
                     /locus_tag="ACL719_001357"
                     /pseudo
     CDS             complement(535..663)
                     /locus_tag="ACL719_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            complement(725..1594)
                     /gene="rsmI"
                     /locus_tag="ACL719_001358"
     CDS             complement(725..1594)
                     /gene="rsmI"
                     /locus_tag="ACL719_001358"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGS0699315.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(1597..1914)
                     /gene="yabA"
                     /locus_tag="ACL719_001359"
     CDS             complement(1597..1914)
                     /gene="yabA"
                     /locus_tag="ACL719_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGS0699316.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1954..2844)
                     /locus_tag="ACL719_001360"
     CDS             complement(1954..2844)
                     /locus_tag="ACL719_001360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGS0699317.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2841..3479)
                     /gene="tmk"
                     /locus_tag="ACL719_001361"
     CDS             complement(2841..3479)
                     /gene="tmk"
                     /locus_tag="ACL719_001361"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGS0699318.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(3699..4496)
                     /gene="proC"
                     /locus_tag="ACL719_001362"
     CDS             complement(3699..4496)
                     /gene="proC"
                     /locus_tag="ACL719_001362"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGS0699319.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(4500..5762)
                     /locus_tag="ACL719_001363"
     CDS             complement(4500..5762)
                     /locus_tag="ACL719_001363"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGS0699320.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADDDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(5772..6881)
                     /gene="proB"
                     /locus_tag="ACL719_001364"
     CDS             complement(5772..6881)
                     /gene="proB"
                     /locus_tag="ACL719_001364"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGS0699321.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6982..8877)
                     /gene="cbpE"
                     /locus_tag="ACL719_001365"
                     /gene_synonym="pce"
     CDS             complement(6982..8877)
                     /gene="cbpE"
                     /locus_tag="ACL719_001365"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGS0699322.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREEIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQYINAASKDYDATVFDIRQDGLVNISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYVVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     HWFYLTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQY
                     MGQWYYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWH
                     KKANDWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVS
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            complement(8880..9767)
                     /locus_tag="ACL719_001366"
     CDS             complement(8880..9767)
                     /locus_tag="ACL719_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0699323.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9757..10218)
                     /gene="lspA"
                     /locus_tag="ACL719_001367"
     CDS             complement(9757..10218)
                     /gene="lspA"
                     /locus_tag="ACL719_001367"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGS0699324.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10215..11123)
                     /locus_tag="ACL719_001368"
     CDS             complement(10215..11123)
                     /locus_tag="ACL719_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS0699325.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLARKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11116..11313
                     /locus_tag="ACL719_001369"
     CDS             11116..11313
                     /locus_tag="ACL719_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS0699326.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(11364..12035)
                     /locus_tag="ACL719_001370"
     CDS             complement(11364..12035)
                     /locus_tag="ACL719_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0699327.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKTFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETIEMIGRQELPYFLLIVCFIGPIAEELIYRGVLMTTFFKNSPWYGDVLLFA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNIYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12089..12310)
                     /locus_tag="ACL719_001371"
     CDS             complement(12089..12310)
                     /locus_tag="ACL719_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGS0699328.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWKMEPVLTLL
                     VHGFCPRDTDDLGYIGGGNHLCKGSAARF"
     gene            12582..13391
                     /locus_tag="ACL719_001372"
     CDS             12582..13391
                     /locus_tag="ACL719_001372"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0699329.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(13572..14447)
                     /gene="aguB"
                     /locus_tag="ACL719_001373"
     CDS             complement(13572..14447)
                     /gene="aguB"
                     /locus_tag="ACL719_001373"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGS0699330.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(14457..15542)
                     /gene="aguA"
                     /locus_tag="ACL719_001374"
     CDS             complement(14457..15542)
                     /gene="aguA"
                     /locus_tag="ACL719_001374"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGS0699331.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKDAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(15539..16666)
                     /gene="nspC"
                     /locus_tag="ACL719_001375"
     CDS             complement(15539..16666)
                     /gene="nspC"
                     /locus_tag="ACL719_001375"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGS0699332.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(16666..17925)
                     /locus_tag="ACL719_001376"
     CDS             complement(16666..17925)
                     /locus_tag="ACL719_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGS0699333.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(17922..18782)
                     /gene="speE"
                     /locus_tag="ACL719_001377"
     CDS             complement(17922..18782)
                     /gene="speE"
                     /locus_tag="ACL719_001377"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGS0699334.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            19038..19203
                     /locus_tag="ACL719_001378"
                     /pseudo
     CDS             19038..19203
                     /locus_tag="ACL719_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5575 a         4446 c         3461 g         5721 t
ORIGIN      
        1 ttttctgatt ttagttctga aagttccaaa aggtgcaacc gtatttacat gaataaactt
       61 atacacttcc caagtcgctg ttttagtcgc ttcatcagcc cactttctca tatgcggttc
      121 aaataattct tcctcactca acgaatcaat cattgcagag atagaatgaa tattttcatt
      181 taattttgct ttcaactctt gcagagataa atgagcgtag gtatctgtga accactgata
      241 taattcacca agttgattcc atttaaattt atccgatggt gtttttactt gaagcccctt
      301 tctttcatct tcttcccatt taagaaccaa gttggtccaa ccaacctgat aagcaaggtt
      361 ttctgctgga gttctgtcaa cttcatcagc tctcttatct tttaaatttt ctggaatatt
      421 atcaaattct aaaatatatt tctcaaatgt tttctctatc tcagctttta gttcttcttt
      481 attttcgtat gttcgcaaaa aatcaccttc tttctgattt atcaatcctt tgatttaaaa
      541 tgaccttatt tttctattta taccttctaa gaacgctata actgctgctc caaccatcgg
      601 tattccgtat gggcttaata aaaaacttaa taccaaagct tctataccaa gtgatacatc
      661 tttatattat catcactaat taagttaaac agaaacagac agaattatta catcctgtct
      721 ccctttattg tttttcttcc cagtcgtggt aggcagcgta gagttgactc ttattccact
      781 ggtaaatctt agcgacttcc ttaatagctt gatttttctt cataccttgc tggatacgag
      841 cttggatttc taagaacaag tcttcctcgt ctttttcttc cacatcctga ctggcacctt
      901 caacaatcag aagacattca cccttgagag acgtttcagc aatgctttcc agcaattcag
      961 aaattgtacc tctttggtat tcttcataga ttttggtcaa ttccctgacc aagacaacgg
     1021 agcggtcacc gtagacttct agcatatttt ccaatgtatc tgccacacga tggggcgatt
     1081 cataaaaaat ctgagtttca ggatagtctt ttttcgagtc gaaaaattgc ttttgctggc
     1141 ctgattttct cggtaaaaag ccgtaaaaga tatgtggctg cggcgctaaa ccactggcaa
     1201 tcaaggcgga aattcctgca gaggcacctg gaactgtaac aactgcaatt tcttcctcaa
     1261 tagctgcctt aaccaaatca tgaccagggt ctgaaatgct aggcaaaccg gcatcagaga
     1321 cctgagcaat actttgccct gctttcaaga aaccaatcaa atcaggaatt ttttccttgg
     1381 cattgtgctc atgaaaactg atctgcttgg tggaaatgtc aaaatgcttg agcaaaagac
     1441 ctgtattgcg cgtatcctca gcagcaatcc agtccacttc tttcaaggtc tggatagcac
     1501 gaaaagtcat atcatctaga ttgccaatcg gcgttgccac tagatacagc ttgccatagg
     1561 gagactgccc cttaaaactt ttttgaattt gcatgcctac tccctgtata acaactcatc
     1621 acagaacata cattcttcgt cctgctctcg acgttgtcca taaaaatcat tacatacgtg
     1681 aaatccatca cggtaaatgc gacggacact ttcacgaaca tgcttggcct tgacaggagc
     1741 atctgcttcc acctcaccca agcgttctcg caacttagaa ttttccaaac gaagagctgt
     1801 attttcctct accaggctct tgagattttt cttgatggct tccacatcgg ccaaggttac
     1861 caataactgt tgggaaaaat catccagcgc gtcaaataat tcttttttgt ccataaaaca
     1921 gccctttcct ttctttatca ttcatttttg agtttatatt tctttcaaga ccagatattc
     1981 catggcattt tgaaagctga cattagcttg ccacattttt ctagcttcta gtaaatcttg
     2041 tagaatcact cttactctta cctgcaagag gtcctgccca cagagaactt caagaatccg
     2101 taaaacctga tcctgttttt ccttatcatc tgccaagttg gctaatttgg caacctgtag
     2161 ataactttct tttttcttag ctactaacca agtcagcagg cgttcacttt catcgaccaa
     2221 ggtccaaaaa cttgcctgat tagccaactt ttctgcttca gctcgcgatt gactaaactt
     2281 agctaaaaga gtcgcttttt tcttaacaag tcccatttgt tctaagagta agataagttt
     2341 ttcttcttgc tttttaaagt ggaagatctg agtccgactt cggattgtcg gtaacatctt
     2401 ttcctcatcg ctagtcaaga agaaaatata aacttcactc tggggttctt cgatgacctt
     2461 gagcagagaa ttggctgcgt tgggatgcat tttatccgct tgctcgatga taaagacctg
     2521 ttgctggctt tcaatccctg cttgagaaaa ctgtcccacc aattcccgaa tgcgttctgt
     2581 cttgatgacc tgattgactg gcttaatcaa ggtgacatct ggaaactctt cctgttcaat
     2641 cagcttgcaa cttcggcatt tctcacatgg taagacgcca actttatccg tacaaaagag
     2701 gctcttagct aaaaattgcg ctatttccaa gcttccaaag aaacctgaaa agagataggc
     2761 gtgattgagc tggtcttgtt ctaagatacg gacaaaacgg tcaaactgag ctggttgcca
     2821 agcctttagt tgatcttgtt tcatttggcc aagcccattc cgtcaaacaa gacagccttg
     2881 gtagtttcca caacttgctc caaagggaga ctagcatcaa tcttgacaat gcgatttccc
     2941 tctttatcca gaagagaaag gtagccttga cgaacttttt tatgcaagtc caacccttcc
     3001 aaatccaaac gattaacctc gcggtcacta ttagcagcaa tacgagccag cccttcttcc
     3061 acctcgatgt caaaatagag tgtcaaatcg ggtttgaggc catctgtcgc aaactgattg
     3121 agccagtcaa tggcttcaat atctaagcca cgaccaaatc cctgataggc aacagaacta
     3181 tcgataaaac gatccatgat gaccaacttg ccagcttcaa gggctggaag aactttttcc
     3241 accaaatgct gtctgcgact ggcaatatag agaagtagct ctgttttagc atccatctga
     3301 gtatgacttg gatccaaaat cacttcccga atcttctccc caatcaagac tccgccaggt
     3361 tcacgggtcg tcaacacctc tactcctttt tcctctaaaa ttggtagcag agcctctaaa
     3421 acactggtct tgcctgctcc ctctggtccc tcaagagaga ctaaaaatcc ttttgacatg
     3481 tctaactcat ttctttttta ctacttctat tctatcaaaa aaataggggt ttgtgacaat
     3541 ctttttgtct tctcatttca tactcaatga aaatcaaaga gcaaactagg aagctagccg
     3601 caggctgtac ttgagtacgg caaggtgaag ctgacgcagt ttgaatttga ttttcgaaga
     3661 gtatcagcca ccataaaaga ggcagataaa actacctctt atttacctag ttcttgtgtt
     3721 cgtttatagg cttgatgaac tgcctccatg actgtgcctc gaaaagcatg cgcttctagg
     3781 cttgctacgc cagcaatagt cgaaccgcct ggactacaaa cttgatcttt caaaactcca
     3841 ggatgttgct ggctttctag gaccaattgc ccagctccta ccacagtttg ggctgccatt
     3901 ttcaaggctg tttctcgtga taatcccgtc tgaacacctg catctgccaa ggcttcaata
     3961 aagagataga caaaggctgg tccacagcct gcaagacctg tcgctgcatt gattaagctt
     4021 tctcctaatt caaccaagag accagccttg gataaaagct gacaaaagag ctcactgtcc
     4081 tcagccctgc aattaggaga caaggcataa ctaatcactc cttgcccgat agaagcaggg
     4141 gtattaggca tcatacgaat aattcggtgt tgacttggga taagacttgc tagtttttct
     4201 aaggtcaatc cagctgccat cgaaatcaaa agaagacttt ctcttttttc aaggatggtc
     4261 tggtattgag aaagcagttc agaaaactga gcaggcttaa ctcctagaaa aatcacatct
     4321 gcttctgcaa acatttcttc attgctggaa gcctgaccac caaagtctgc aatgaaagca
     4381 tctaccttag cttgactacg attggcaagg agaatctcat ctgacgtcct agtctgcaag
     4441 acagattttg ccaagctagc acccatattc cccaaaccga taaatccaat cttcatctct
     4501 tactccctta tctgcccatc accagcaacc acatacttgt agctggtcaa ctctttcaag
     4561 cccattggac cacgcgcgtg caatttctga gtagaaatcc ccatttcaca accaagacca
     4621 aattgtcctc catctgtgaa acgagttgag gcattaacat acaccgctgc agagtccact
     4681 tgatctgtaa agtatgctgc agcttcagca ttttccgtca caatagcatc cgaatgatgg
     4741 gtgctgtggg attcaatgtg cgcaaccgct tcttctaaac tgctcacaac cttaacagca
     4801 aggacatagt ctaaaaactc ggtgtcaaag tcttgggtct cagctgcttg acctgaaaca
     4861 aactggcttg ctttgctatc caagcggaat tgaattggtt ccaacccagc ttcttttcga
     4921 tctgcaacca gcacttgctc caagcgagga aggaagcttg ctgccttgtc ttcatgaacc
     4981 agcagaacct ccatggcatt gcagacagaa ggacgactgg ttttggcatt gttgatgata
     5041 gacagtgcct tgtcgtcatc tgcgtcctta tcgacataaa catggacaat cccagttcct
     5101 gtctcgataa caggcacaat ggcattctca accactgcat tgatcaagcc agctcctcca
     5161 cgaggaatga gaaggtctag atagcccttg gccttcatca tagcataact actttcacgg
     5221 ctagtatcct ccaccagttg aatcacattt ggatgaatag tagtcgtctc caagcccttc
     5281 ttcaaggctg tgacaatggc atgggttgtt tgataggcat ccttaccact acgaagaaca
     5341 accgcatttc cactcttaag agtcaaagca gccgcatcag acgtcacatt tggacggctt
     5401 tcatagataa taccgatgac acccatagct acacgttttt ttgtgataac caaaccattt
     5461 tcaagctgac ttgtttctaa aacttcaccg attggatctg gtaaggcaac cacttcacga
     5521 attcctcttg ccatcgcttc tatacgatct gcatccaaat aaagacgatc caacatcaca
     5581 tctgagattt tccccttagc cgctgccata tcgagggcat tagccgctaa aatttcctca
     5641 gtagcagcca ctaagtgatc agccatggct agcaaggctt ggtttttcac ttcttcacta
     5701 gctgtgttaa tcgatttttt aacagcctgt acctgttcaa attgttcttg tctactcacc
     5761 atagtttacc tctaaaattc tgtaaaaagt agttggattt caggagtaat ggaaatccag
     5821 tcgtcacggt aaatcaagac acccttggct ttttgagaac gcaacatatc ctccaaagca
     5881 gatgctccaa attgcacgcg tccttttcca agtgattttc cactttcctt atcaaatact
     5941 gtcacgatat caccgtaaga aaagactcct tctgcttcaa cgataccaga taagagaaga
     6001 ctctttccat gttgagagag agcttccgca gcccctttat caacccaaat agaaccttga
     6061 ctctgagcat agaaggcaag ccattgtttc tgggtacgaa gccccttctc ttgagcaaca
     6121 aagtaagaac catcctcggt ctcctctgcc gcctcaatca tggaatctga tttcaaggat
     6181 gagcagatat aaacaggaac tcctgattcc gtcgcgatag ttgcagcctt gattttggtt
     6241 aacatacccc cagttccgtt tgacgaacca gctccaccag ccatatcaat aatctcacga
     6301 ttgatggtct cgattctctc caagcgtttg gctcttggat ctgaattagg atttccagta
     6361 tagagaccgt ccacatctgt caagaaaact aaaaggtctg cttggaccat cgccgctact
     6421 tgagcactta gagtgtcatt gtccccaacc ttaagctcat caataacgac actatcattc
     6481 tcattgatga taggaattgc cccacggttg agcaaaaccg acaaagcctg atgggcattt
     6541 ttataacgac gcttatccac aaagtcatct tgggtcagca agatttgtgc agaaacgatt
     6601 tgacgcaaga gaagattggt cgtatattct tccaacaaaa gtccctgccc taccgctgct
     6661 gaagcctgtt tatcagcaat tttagtcgga cgctttttaa atcctaaggc tccaaaacca
     6721 gccgcaatgg cacctgaaga caccaaaatc aactcatgac cagcctcgtg cagcatagcc
     6781 aactgctggg taatatcctt taccttacta cgtgataaac ttccatcctc atttgtcaga
     6841 gaagaagtac ccaccttaaa gacaatccgt ttgtatttca tagtctcact ccgattattc
     6901 atatttatcc attataacat gaataacagg aaatagaaaa gagttgatag gaaaaagaag
     6961 gccctaaaac cttcttttct actactgttc tgattccgat ttgtttacac tcgtttcaga
     7021 gctttgtgaa gttgaggatg tcaaatcttg actagttgaa aaatttgtaa cactttcatg
     7081 ttgactcgtt tctttatttt caagatcctt tttcactact tctttggatt cttttatttc
     7141 tgaatgactt gtagttttaa ttgtagcaga tttctcgatc gaaacatcca ctaaccaggc
     7201 accacttgaa tccacagtat aaccttctgg tgtcttaccg ttcacaagta attgaccatt
     7261 ttctttcaag aagtaccatt tatccttgtc cttgatccaa cccacagctc gtgaaccgtc
     7321 tgtcttgtag aaataccaat catttgcctt tttatgccag ccttgcttca tagctcctga
     7381 atcttccaga taataatgtt gaccagcaac ttctatctca cctgtcttca tgacaccagt
     7441 agaatccata tagtaccagg tttctttatc ttttacccag cccattttca tttcccctga
     7501 tggagcaaag taataccatt gccccatata ttgaagccat cctgtcttca tagaaccatc
     7561 aacatccaaa taataccact gctctttatc ttgaacccaa ccaatttcca tctgattttc
     7621 tttgttaaaa taataccaga ttccatcaat tttcttccaa tttttagcag aagcaccaga
     7681 gtctgtcaaa tagaaccaat gattgttcca ttttttccat tgattctgta ataagatacc
     7741 cgtttggtta aagtaatacc attcaccctt aatttcattc caaccgacaa catactctcc
     7801 tgtagaatca ggcgcttgat accaccagtt tccatatgaa ctcttatgcc aaccagcttg
     7861 aaaacttgga atcggcttgt aggaagttga aatgtttact aaaccgtctt gtcgaatatc
     7921 aaaaactgtt gcatcatagt ctttgctggc tgcatttata tattgaattc ccattgattc
     7981 aagccaacta atatattccc tatttatttc ttgaccgcta tcatacgaac caattggtga
     8041 ggaggaagtt tgaacaatca aactcggact caaattttta atgaaatcct tggtatttga
     8101 tttgttggta tcatggtgat gattaaactt catcaaatca acttttccaa tgagaggacc
     8161 atacttgtct tctgctccat gaacattatc taagtcgccc ccaaggtaaa ttttcttgcc
     8221 attgactttt accacactaa tcaaggaatt ggaattatca tcccaaattt tctttaattc
     8281 acctgatgaa tcggtttcat tttcatagtt atagagttga atatccatgt ccccaaactg
     8341 aaaatgagca tccccttgtg tgatattttg aataactgaa acaccttttt ctgaagcagt
     8401 ctgtaaaacc ttatcataac cgtacagatt atcccataga cgttcagaat tagtaatacg
     8461 attatcacta tatttcttaa gatagactcg gtcaactgga taggtagaca gtaattcatc
     8521 aacatttcca atatgatcac tgtgggtatg ggtcaccaaa ataaaatcaa gtttttggac
     8581 acccaattcc ttcaaacgac gaaagacacg gtctgttaga acatgcttat aagacgtttc
     8641 aatttcttct ctccatggat agcgagaatc acttccatct gggaaatcat aatcttctcc
     8701 tgtatccacc atggcaaaat gtccattgct ttcaagaata atcgcatcac tgccaccttc
     8761 ttgaacattg ataaagtgga ttttatttcc tgaactttct tgagcctgaa cattcccata
     8821 ccatgacaaa cctaaaaaag cgcctacaag tgctaaacta gttaatttct ttttcattct
     8881 tactttctca atctctccaa ggtttcctta aaaatctctg ggatatctgc tttaaattcc
     8941 aaggtcttac ctgttctcgg atgagtaaaa cctaaagtct tggcatgaag aaattgtcca
     9001 tgtcctttca aagtcttgcg aggaccatag acctcatcac cagcgactgg atggccgata
     9061 taagccatgt ggacacggat ttgatgagtg cgccctgtct ccagttgcaa ctctactaag
     9121 ctataatcgc caaagcgttc caagacgtga aaacgcgtca ctgcaggctt ccctttagca
     9181 gttacagcct gtttcttacg gtctttttca ctccggccaa tcggcgcttc aattacacca
     9241 cgatcattag gcagatttcc atgaacaatc gcccaatatt tgcggagaga ctttttatcc
     9301 ttgagttctt gggcaagtgc tagatgcgca tcatcgtttt tagcaatcat gagaagacct
     9361 gacgtatcct tatcaatacg gtgaacaatt cctggacgca gaaccccatt gatacccgac
     9421 aagtccttaa tatgatacat gagggcattt actagggttc cactggtatg accagcactc
     9481 gggtgcacaa ccattccctg aggtttgtta acgacagcca catcctcatc ttggtagact
     9541 atttctagcg gaagatcctc agccacatac tctaatacct ctggttctgg cacatggtaa
     9601 gtgacgacat caccctcttg gactgtgtat ttagctttct tgacttgacc attgaccaag
     9661 acctggcctg atttaatttg ttcattcgca agactgcgtg ataattctgt caaatctgac
     9721 aaagccttat ccaaacgcag accaccagtt tcaattttaa tttccattta tttcctcttt
     9781 tagcattgca atcaataaaa taatcactcc aaccgtcaga tagctatctg ccacattgaa
     9841 aattgcaaag ttgataaagt caaggtggaa catatccaca acaaagccct gactggccct
     9901 gtcaataaag tttccaagac cacccgcgat tatcaaagtc aaacccaaga ccatccagaa
     9961 tgagtcctcc atgtgtttat gtaaatacca aatggcacct atcacgacaa ccagagtaat
    10021 gacagcgaat aacagctgct gatcttgtaa gatagaaaag gctgcacctc gattttgcag
    10081 gtaggtcaag ctaacgaaat tgggaatcca ggagcgcact tcacccagtg gaatctgctg
    10141 gacgatatag gatttgacca actgatccag cccaattaaa agcagtacaa tgactgccac
    10201 tattgctctt tttttcatga tttcctcttt tgatcaaaat attcttgcat gacttctacg
    10261 aagagagtcc cagcttgact aagctccact tcttcacgtt taacatagac catgcggtta
    10321 tctaggttat ccttgagacg aataactgta atgccattaa cactgtcact atctaaaaat
    10381 ccagaacctg tcgcataggc gtccgtccgc tccaaaatac cattcaaggt ggcacggtct
    10441 gtcacattaa acatctgtga gctagcgctg gtatcgacaa agttctctga ataataaagg
    10501 tactcgtctt tctcttgagt gaaacgaacc gttggtaaat ccgctaaatc ctccatgact
    10561 aattcctctt tccgagctaa aggatgaccc tcacagagat aaatatgggt atggaaagga
    10621 atcaattcga tgacctccag acctaacttt tcaacccgtt gcataatccc ctttttattt
    10681 tgattgttga ggtagataat cccaatctca ctatgccctt gcgccacttc atctaatatt
    10741 tgaacagtag ttgattcaaa aatacggaag ttcttatagt caggatagcg ctctgaaaag
    10801 gccgtaatag ttggtggtaa gaagtcatag tgctggctag caacggaaaa ttcatctttt
    10861 tcttcttcag gattggcata ctgattttga aaaatatcaa atcctttaac caattcttgc
    10921 gctttttcat aaaattccat cccacgacgg gtcaagaaag tccctgagct ggtccgacgg
    10981 aaaatcttaa agcccaactc tttttccaaa tcacgaacag aaatagacag actcggctga
    11041 ctaacataca tcttttcagc agcttcacga aaagtaccac tattggcaat agccacaaca
    11101 tagcgtaatt gttgaatgtt catcttctac ccccaacttc tttatctttt tattatacca
    11161 tattttagaa gttttccaaa aaggaaaaaa gaacatccta ttcttcttaa ctatcttcac
    11221 tatctgcctt tctcacgcca atcttatttt caaaatcaat tcaaaaaaat aagtggctac
    11281 acaccacttc agtatcggaa agaaaaacgt tgactatttg tgaaaaaaga aaatgccgga
    11341 aaattccgac attttttagt aagctaactt cctgaaaata ggagtaacca aacatcccag
    11401 aaagcagtga tattgatgag aatatgaact agtattggat agtagatgtt tttagtcatg
    11461 cgatacaata gagccaaaat aagacctccg ctagcataaa tgaaaaaagc aagaggagtt
    11521 aaagcaaaat tgatatgaat ataaccgaaa ataatagcaa aaagcaagac atctccgtac
    11581 caaggcgagt ttttgaaaaa ggttgtcata agcacaccgc gataaatcaa ttcctcagca
    11641 atagggccga tgaagcaaac gatgagcaaa aaatagggta attcctgtct ccctatcatt
    11701 tctatcgttt cattcaaaga aatttgattt gaagacaggg atatgaaata cgaaaagaga
    11761 aagtcagaca tatacgaagt gatgtaaccc agtaaaaggt agataaaata cctcagctgc
    11821 cacttgaaat gaaaagtttt ctttcctgca aaaactagta gatagatgac cgctaataaa
    11881 agaactccac tctccatcaa aagcagaatc tgaaaaaatt cacgacttgc tggtagatag
    11941 ggctttgcga gataattgaa aagcctccaa aaccaagttg atttgtaaaa aattaaggac
    12001 aatgctagta aaatttgaat agcccgtttt ttcatattaa attctctgct ttctccactt
    12061 gtttcttcat attactaata aattttactt aaaatctcgc agcacttcct ttgcaaagat
    12121 gattacctcc tccaatatat cctaaatcat cggtgtctct gggacaaaat ccatgaacca
    12181 gcagagttaa tactggttcc attttccaag gataataacc attaggttct ttaccatagg
    12241 gatctgctcc accttgtatc agctctaatt cttcatttgt tagatttata ttttttgtca
    12301 ttaaaatcat gatatatttc ctccgttatt gtttagatta gttgagtaat ctgctactca
    12361 tacctactta caaaaaaact attatattaa gttggttttt taatttgtct agttgcaaca
    12421 ttgacaaaca tagtatagca tatctttgcc aaatatcctc ttcaaatcat gaattgtcat
    12481 caaaacatct taaactataa aatcaattag tctcaagctt tctatcaatt tcttatccaa
    12541 aatatgctat aataatacca aaagataaag aaggaagacc tatgattaaa ctactagcct
    12601 tggatatgga cggcaccctc ctcaatgaag ccaaggaaat cccacaagct cacattactg
    12661 ctattcacaa agccattgaa aaaggtgtca aactggttct ctgtacgggt cgcccccttt
    12721 tcggtgtcct cccctactac aaaaaactgg ggcttgacct ccagaacgaa tatgttattg
    12781 ttaacaacgg ttgttcaact caccaaacca gtgattgggg tctagttgac tggaaagaac
    12841 tgagtccagc tgacatcgaa tacctctatg accttgctga aaagagtgat gttcagttga
    12901 cactttttga cgagtcacat tattttgttc tcggtggcaa gcccaatcaa gttattgaaa
    12961 atgatgctaa actagtattt tcagacctga ctgaaatttc tcttgaggaa gcgactagtg
    13021 gaaagttccg gatgttccaa ggtatgtttt taggaacaaa agaacaaaca gacgattttg
    13081 agcagcgttt tgctgaagag ctttgccaac gattcagtgg agttcgttcg cagcctgtca
    13141 tttatgaagc aatgccactt ggaacgacaa aggctactgc tctttcacga ctagctgaga
    13201 ttttgaagat tgattcctca gagattatgg ccatgggcga tgctaataac gatatcgaaa
    13261 tgctccagtt tgcagggctt gggattgcaa tgggaaatgc cagcgattat gtcaaatctc
    13321 ttgcggatgc cgttacctca agcaacgaag aagacggcgt tgcgcgtgct attgagaaat
    13381 atattctata aaaaagaaaa gcaaatagac agaagttact gctatttgct tttttgctaa
    13441 tattttaaaa tacggactaa tcaatagaca agaatagcga atcataaaaa tcgatttact
    13501 agatgccata gtatcttata gttgctaata agaagttagt ctagcagaat gaatctctca
    13561 tttctcccac actaatctgt aatttgtcta tacatttctg gtcttctatc tcgaaacaag
    13621 ccccagttta gacgttcact tgctcccttg tctaggtcat aagtagctaa cagaacagct
    13681 tcttcttgtc tttcagctcg ttctagaata gctcctgttt catccgtcat aaaggaggaa
    13741 ccgtagaagt caagactgga actctgtccg ccattttcct cactaggagt aacctcctct
    13801 aaaccataac gattggctgc gatgactgga acaatattcg ctgctgcgtg cccttgcata
    13861 gtacgttgcc agtgaccaca actatctgta tccaaaattg gctctgaacc gatagctgta
    13921 ggataaaaga gcaattcagc accattcaat gcaagacagc gcgctgtttc agggaaccat
    13981 tgatcccaac agataccgat accaatctta gcatagcgag tattccagac cttgaaacca
    14041 gtgttaccag gcgtgaaata gaatttttct tgataataat ggtcatctgg tatatgggtc
    14101 tttcgataaa cgcccagcac ttccccatct gcatcaatga cggcaataga gttatacaag
    14161 acattaccat ctttttcata gaaactgatt ggtaaaacaa cttgtagttc cttagcaatc
    14221 accttaaaat gctgaatggc agtattttcc gctacagatt gggcatactg gtagtagtca
    14281 tactgacgtt cctgacagaa atagggatgt tcaaacaact cgggcaagag aataatttgg
    14341 gctccttgct cagcagcctg acgtactaaa cgctctgcgg tttggatatt tgttgccaca
    14401 tccttagcgc attgcatctg aatggttgca actcttacat ttctcatctt tttctcctat
    14461 tctggaattt gttgggtgat acagtggata ttgccaccac ctaagagaat atctctggct
    14521 ggtattccga caactttacg gtctgggaaa cacttgctga ggatatctaa ggccacttgg
    14581 tcgtttacat cctcaaactg tggaaccaag acagccttgt tggcgatata aaagtttacg
    14641 taggaagctg ctagtcgttc acctgcgtat cgctcttctt ctccttcttc atagatgtag
    14701 cctggcaaat cttcttctgt cacaacttgt cgaactgcag ggataggcaa tttatgaatg
    14761 gtgaagtgac aaccttttgc atctgtttcc tgttctaaga gttcgagatc tgcttttgac
    14821 atggcatact ggggatcatt ttcgtcatct gtccaagcca aaacaacctc agcaggacca
    14881 acaaaggcag caacattatc gacgtgttca ttggtttcat cctgataaat accataagga
    14941 agccaaataa ctttttcagc accaagactt tctaataatg tgttttcaat ctcctcttta
    15001 gtcaagttag gattgcgacc aggactaagc aagcaacttt cagttacgag aatagttcct
    15061 tgaccatcgc tatggattgc gcctccttcc agtacaaaag gtttagcatc atagacaggc
    15121 ctttccaagg cctcagcaaa acgactggct acttggtcat cctcttcata atcttgataa
    15181 agaccatcat aggtgcctcc ccaagcattg aaggcccaat ccacggctaa tttcttacct
    15241 ttgtcattga cgagaatggt tgggccagta tcacgcgccc aggcatcatt ggtgggaatg
    15301 tctaaataaa caaccttgtc tccaagatag gattgggctt cagatagata ggcctgctcc
    15361 accaaaagat agactctttc cccttctgct atagtctcga tgatctgagt aaatgctctt
    15421 ttagcatcct ttccttgaaa aggccatgat cctggtcgag tcggccatat catgagggta
    15481 ccatgatggg gttcgtactc tgctggcatg tgatagccta atttttttgg actgtccatc
    15541 atgataatct ccctttaaag tcttgatagc caaaagcttt gagtaagcta cagtctccct
    15601 gttcgtccat gagatagaga cttggcaatc caataccatt aaaggtatta tttttgacaa
    15661 aagaataaat ggccatgtct tgaaaataaa gtctgtctcc gatttggact ggattttcaa
    15721 aactataatc accaatcaca tcgcccgtca gacaggtatt agaagaaagt ctgtaggtat
    15781 gggctttttc ctgtgcctca aagccatttc tcaaaggtgg acgatagggc atctcaagta
    15841 catcaggcat atggcaggtc gcagaggcgt ctaaaaccaa gatttccata ccgttttcta
    15901 caatatctaa tacctcagtt gctaaataac ccgcattaag cgcaatggct tcaccaggct
    15961 cgatatagat ttcaagattg taagtttttc ggatacgctt gatttctgaa atcagcaaat
    16021 ccacatcgta accttctctt gtaatatgat gaccaccacc catattgagc cattttacct
    16081 catgtaagta gggaccaaac tgttcttcta ctgctttcaa agttgtttgt aaatcatctg
    16141 ctccctgctc gcaaagggta tgaaaatgaa gtccgtcaac caaatctagc aaatcactcg
    16201 gaatcttgtc tatagtaact ccaaagcgag aacctggtgc acaagggtca tagagcgcgt
    16261 gatctccttg agttgaacac tgagggttga ggcgcaaacc aacactgaca ccagcctctc
    16321 gacaacgcga accatgctta cgcaactgtc tctctgagtt aaagacgata tggtctgtta
    16381 tcccaagcaa ttcctccatg tcctcatcct tgaaagctgg tgcaaaaaca tggacttcac
    16441 caggaaattc ttcccttgcc aatttggcct catagagtcc actagctgtc gtacctgata
    16501 gatactggct aatcaaggga taagttttgt agagggaata tgccttctgg gcaagcaaga
    16561 ccttgcaacc ggcctcttct tgtacatatt gtagaatgcg gcaattagct tctaacttgg
    16621 ccaagtcaat aacataggct ggtgttggta cttgttctaa cttcattagt ccaccatttg
    16681 tggattttca accacaaccc atggcaaacc atactcattc aaagcttcca tgaatggatc
    16741 tggatctaac tcctcaaggt tatacactcc agcttgtttc caagttccgt tcatgactaa
    16801 ttttgtccca atcatggctg gaactcctgt cgtataagaa atagcttgcg aaccaacctc
    16861 tgcgtaacat tcctgatggt cgcagacatt gtagatatag atagtctttt tgacaccgtc
    16921 tttgacacct gtaaagatac atccaatatt ggttttacct acagtacgtg gaccaaggct
    16981 agcaggatct ggaagcaagg ctttcaaaaa ttgaattgga acaatttctt ggccgttaaa
    17041 gttaatggta tccgtacgaa ggagtccaac attttcaaga catttcatgt gcgtcaagta
    17101 agattgacca aaagtcataa agaaacgaat gcgtttgaca cctggaatgt tcttggccaa
    17161 tgattcgatt tcttcatggt gaaggagata catatctttt tgtccaactt gagggaaatc
    17221 atactcacgc ttgatagaca tagcttcgac ttcgacccat ttcccatctt cccagtaaga
    17281 acctggcgca gaaacctcac ggagattaat ttctggatta aagttggttg caaatggata
    17341 accgtggtca ccgccattac agtctaaaat gtcgatataa tggatttcat caaaatagtg
    17401 tttgagggca taagctgaaa agacactagt tacacctggg tcaaaaccag aaccaagaag
    17461 agcagtcaag cctgcttctt tgaatttctc ttgataagcc cactgccatg agtagtcaaa
    17521 gtaggctgta aaaccaagtt ccttacaacg tttttcgtag atagcacgcc actcagggtc
    17581 ttctgtgtct tctgcttcgt agttggctgt atcgatatag tgaacacctg ttgccaaaca
    17641 agcatccata atggttaaat cttgataagg cagagctaca ttcaaaacag cttctggttt
    17701 gtagctttca atcagagcaa tcacttcttc aaccttgtca gcatcaagag cagctgtctc
    17761 aatctttgta cttgttttgc cttccagttt agctttcaag tcatcgcatt ttgacttggt
    17821 acggctagca atcataatct ctgtaaatgt ttcgctatct tgacaaatct ttgaaatagc
    17881 aacttgggca acgcccccac aaccaataac tagtaaacga ctcatttttt tccttcctct
    17941 tcttctaaaa tgtcctcaac atacttgggc aacataaagg ctcccacgtg taagtttgca
    18001 gtgtagtatt ctgtgaaaag ctggcgtttt ttccagcctt ccttgtcaaa atctttgaca
    18061 gggtggtatt ttttcgatgc aaatccaaac aaccaatagc cagctgggct agttggaata
    18121 tgggcctgat aaacccgact gattggaaag gcttgattga ccttgcggtg catgcttcgg
    18181 caggccgact catcctcgtc aaagaacgga ctcccatgct ggtaaatcat gatgccgtct
    18241 tccttcagag ctcgataact attgccgtag aattccttgg taaagagtcc ttccgtatgg
    18301 ccaaatggat ctgtcgcatc gttgatgata atatcatagt catattcgca gtttcgcaaa
    18361 aagcgtagcc cattttggta gtaaatggta acacgaggat catctaggcc tgcagcaaag
    18421 tctgggaaat actcacgaca gacctcgacc aacatctcat ccggttccac gatatcgatt
    18481 tgttccagtt caggatagag tgttaatact tgggcaacac cgccgtcacc acccccaata
    18541 accaatactt tctttggatt tgggtggaca gccatgggaa cgtgaacggt catttcattg
    18601 tagacgaaat catccgcatc tgagaacaag acatggccat ttaaaatcag tattttccca
    18661 aaagctggcg tatccaagac ttcgatatcc tgccattcac tttttccagc gtaaagttgc
    18721 ttggctgttc tcagagacaa tttgacatct ggagtatgaa cttcagaaaa ccataaatcc
    18781 atctatttct ccttatatta tatttagcgc agttagctac tgccaaagcc caagtggtat
    18841 acttggaata agccactgtg gattagttca tttcctccaa tcacttgacg atttccgaaa
    18901 gggtctaacc acagcttttt gtttcaattg gtaatgagtt tgataacgca tagacgtttt
    18961 atctcgtcta aggtaacaag gcaaaaagtt cgtggataac caagtagact tgaaatacta
    19021 tatctggagg tactcccatg ctttatgtgg gcattgatgt cgctaaaaat aaacacgatg
    19081 ttacagcctt gaatgttcga ggaaaactgt tcttaaacca ctcacttttt caaataataa
    19141 agctggtttt gaactcttag atctgtctct tcgacaactc aaccaagact gtctcatcgc
    19201 tct
//
LOCUS       DBERCA010000036        19108 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.036, whole genome shotgun sequence.
ACCESSION   DBERCA010000036 DBERCA010000000
VERSION     DBERCA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.036"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="ACL719_001379"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="ACL719_001379"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS0699335.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="ACL719_001380"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="ACL719_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGS0699336.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="ACL719_001381"
     CDS             2285..2818
                     /locus_tag="ACL719_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGS0699337.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(3259..4014)
                     /locus_tag="ACL719_001382"
                     /pseudo
     CDS             complement(3259..4014)
                     /locus_tag="ACL719_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(4152..5774)
                     /gene="groL"
                     /locus_tag="ACL719_001383"
     CDS             complement(4152..5774)
                     /gene="groL"
                     /locus_tag="ACL719_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGS0699338.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(5790..6074)
                     /gene="groES"
                     /locus_tag="ACL719_001384"
     CDS             complement(5790..6074)
                     /gene="groES"
                     /locus_tag="ACL719_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGS0699339.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKAAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(6344..6739)
                     /locus_tag="ACL719_001385"
     CDS             complement(6344..6739)
                     /locus_tag="ACL719_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS0699340.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(6817..7578)
                     /locus_tag="ACL719_001386"
     CDS             complement(6817..7578)
                     /locus_tag="ACL719_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0699341.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(7611..8237)
                     /gene="ytpR"
                     /locus_tag="ACL719_001387"
     CDS             complement(7611..8237)
                     /gene="ytpR"
                     /locus_tag="ACL719_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGS0699342.1"
                     /translation="MIFTYNKEYVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(8253..8570)
                     /locus_tag="ACL719_001388"
     CDS             complement(8253..8570)
                     /locus_tag="ACL719_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGS0699343.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(8567..8866)
                     /locus_tag="ACL719_001389"
     CDS             complement(8567..8866)
                     /locus_tag="ACL719_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGS0699344.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDLFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFIYENEDLVYEEEVL"
     gene            complement(9064..9144)
                     /locus_tag="ACL719_001390"
     CDS             complement(9064..9144)
                     /locus_tag="ACL719_001390"
                     /inference="COORDINATES: protein motif:HMM:NF039231.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699345.1"
                     /translation="MASSGKMLRNTYTPDSYYVGNLGAWQ"
     gene            complement(9151..9342)
                     /locus_tag="ACL719_001391"
     CDS             complement(9151..9342)
                     /locus_tag="ACL719_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HGS0699346.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(9503..10177)
                     /locus_tag="ACL719_001392"
     CDS             complement(9503..10177)
                     /locus_tag="ACL719_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699347.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(10183..10629)
                     /locus_tag="ACL719_001393"
     CDS             complement(10183..10629)
                     /locus_tag="ACL719_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0699348.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(10731..11246)
                     /locus_tag="ACL719_001394"
     CDS             complement(10731..11246)
                     /locus_tag="ACL719_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS0699349.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(11243..11605)
                     /locus_tag="ACL719_001395"
     CDS             complement(11243..11605)
                     /locus_tag="ACL719_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HGS0699350.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(11671..12702)
                     /locus_tag="ACL719_001396"
     CDS             complement(11671..12702)
                     /locus_tag="ACL719_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699351.1"
                     /translation="MDFLLMGALFIVLPQTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATVHAGQKVAIVGPTGTGKTTIVNLLMKFYEIDKGSIHI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            complement(12709..13530)
                     /locus_tag="ACL719_001397"
     CDS             complement(12709..13530)
                     /locus_tag="ACL719_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699352.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPA
                     SMMMGMPFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLM
                     RTTNDITQVQMLITMDLQVVTCGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            complement(13547..14017)
                     /locus_tag="ACL719_001398"
     CDS             complement(13547..14017)
                     /locus_tag="ACL719_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS0699353.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(14141..14857)
                     /locus_tag="ACL719_001399"
     CDS             complement(14141..14857)
                     /locus_tag="ACL719_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0699354.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(15738..17147)
                     /gene="ply"
                     /locus_tag="ACL719_001400"
     CDS             complement(15738..17147)
                     /gene="ply"
                     /locus_tag="ACL719_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGS0699355.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQHEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
     gene            complement(17158..17568)
                     /locus_tag="ACL719_001401"
     CDS             complement(17158..17568)
                     /locus_tag="ACL719_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699356.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTVIPTSYLARRRRN
                     VSEELYEEILDYLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(17561..18169)
                     /locus_tag="ACL719_001402"
     CDS             complement(17561..18169)
                     /locus_tag="ACL719_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699357.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(18182..18640)
                     /locus_tag="ACL719_001403"
     CDS             complement(18182..18640)
                     /locus_tag="ACL719_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGS0699358.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            18765..19108
                     /locus_tag="ACL719_001404"
                     /pseudo
     CDS             18765..19108
                     /locus_tag="ACL719_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         5646 a         4244 c         3478 g         5740 t
ORIGIN      
        1 ctttttatgt tttgtcaaca ccttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tggccctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgataaa
     1681 aacattgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcaaaaac tagctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gctaataaac tattgaaaaa atatgaggga
     1921 aaacttccat ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc ttgttttttt ggtagtataa aataaattgt
     2881 gtaaacacaa aaaggaataa atccattata gcagagttac aacacattac tagaaagaga
     2941 tttattccta cgactcagtt tggcatgctt aacttcctaa ctcaaaaaca aagcatcgat
     3001 taatttatcc gttcttcttt tgaaacagat ttgaactgat aattgctcgt tttaatttaa
     3061 gaagctcttt tgagcaaaac ttcatttttt taagagtcca acaatttctg tctatcgctt
     3121 ttctactagc aaaaaaaact accttgaggc aagttttcat ttatatatct aatttcgtaa
     3181 tagtatagta aaatgaaata aggacagtca aatcgatttc taacaatgtt ttagaagtaa
     3241 aggtgtacta ttctagtttc aatctactat aaatttgata tgagaatagt ctttctcaac
     3301 atccttaccc aacaagaatg ctgtagcgtc cttcttaact tgaaccaggt caatattctg
     3361 ggcttgggct gcatagacgc agccacaata acattgacga tagatatcat actcctcaca
     3421 catctctact gaacgtttgt agccttgatt tttcttgaaa tcgttgggaa gatagtgggt
     3481 cgtgtaaatt ttttgcacat cgattccgat gctgttgatg gtttgagaat tcttatgagg
     3541 actgatggtc aaggctgaac caaagtagtc aaagcccaag tccatagcca cttgcgctgt
     3601 tttatccagt cggtagtcaa aacaaacctt gcaacggtcg ccaccttcgg gctcctcctc
     3661 tagtccccta actagttttc ggtattaatt gggttcgtag ggagcttcta ggtactgaac
     3721 cgtatttcct gtccgctcat taaaatcact aacaaatttc ttggtgacat agacccgctt
     3781 atggtattct accttgggat ggatattaga attggcaaaa tagatggtca catctgcata
     3841 cttggtcaaa tattctagtg tataggtact acaaggggca cagcaaacat gcacgagaat
     3901 ggttggccgt tgctcatttt tctaccatac ttctaccatt ttctgcatga cacggtcata
     3961 attaatcttc tgattggggg gcatcttgca cagaatttct tctacatcga tcatgttctt
     4021 ctcctttttc tagtcttatt ttatcatata agttaggaga gctcaaatca atttagaagt
     4081 gaatattata aaaagaaggg ttagtcattc cctccttttg tgttttttat aagttgtttt
     4141 ctatagaaag cttacatcat cccgcccatc atacttggat ccattgctgg agctggggct
     4201 actggttctg gtttattggc tacgactgct tctgttgtca aaatcaagct tgctacagat
     4261 gctgcatttt gtagggctga acgactcact ttaactggat caatgatacc ttgatcaatc
     4321 atgttaaccc actcgccagt tgctgcgttg aatcctatac caagctcagc atttttcaaa
     4381 cgatcgataa cgatagatcc ttcaaatcct gcattgtgag caatttgacg aacgggttct
     4441 tccaaagcac ggagaacaat attacgtcct gttgcttcat ctcctgtcaa ttccaaggta
     4501 gcaacagctg gaatcacatt ggcaagagct gttccaccac ctgcaacaat accttcttca
     4561 acagctgcac gagtagcgtt gagggcatct tcaatgcgga gtttcatttc tttcaactca
     4621 gtttcagttg cggctccgac cttaataacc gctacaccac ctgacaattt ggccaagcgt
     4681 tcttgcaatt tttcacggtc aaattcagaa gttgtagttt cgatttgaga cttgataacc
     4741 gcaacacggt gagaaatcgc ttcaggattt cctgcacctt ctacaataac cgtgctatct
     4801 ttgtccacgg tcactctcgc tgcttgacca agagcttcaa ttgtcgcatc tttcaactca
     4861 agaccaaggt cttctgtgat aactgttccg cctgttaaga tggcgatatc ttcaagcatg
     4921 gctttgcgac ggtcaccaaa accaggtgcc ttgactgcta ctacgttgaa ggttccacga
     4981 atcttgttca aaacaagagt tggaagagcc tcgccatcca catcatccgc aataatcaag
     5041 agtggacgat tgctttggag aatgctttcc aaaagtggca agatttcttg gatattagaa
     5101 attttcttgt ctgtaatcaa aatgtacgga ttttcaaggt cagccaccat tttttcgcta
     5161 tcagtcacca tgtactgtga aaggtaacca cggtcaaact gcattccttc tacgacttca
     5221 agctctgttt ccataccacg tgactcttcg atggtgatga caccgtcttt gccaactttt
     5281 tccattgctt cagagatgta ctcaccaact ttttcagaac gagaagatac ggctgcaact
     5341 tgagcgatag cttctttatt ggcaacaggg atggcgttgt ttttcaaagc ttcaactgct
     5401 gcggcaactg ccgtttcaat cccacgacga ataccgattg gatttgcacc tgctgtgacg
     5461 tttttgattc cttcacggac gattgcttgg gtcaaaacag ttgcagttgt agttccatca
     5521 cctgcgatat cattggtttt tgaagctact tctgatacca atttggcacc catattttca
     5581 aaatggtctt ccaattcgat ttctttggca atggtcacac cgtcattggt aatcaaaggt
     5641 gaaccgaatg acttttcaag aacgacattg cgaccttttg gtcccaaggt tactttaaca
     5701 gtgtctgcaa ggatatcgac accacgaacc atggctgaac gggcatctga tgaaaattta
     5761 atttcttttg acatacttac tttctccttc tattcctcaa tgattgccaa aatgttagct
     5821 tcgcctacga tgatgtactt ttcatcgcca tctttgacat caagacctgc gtgggcttca
     5881 actaagacac gatctccagt tttaacactt ggagcaacca agtcaccgtt caaggtacga
     5941 acaccttgtc cagtagccac aacttgagct gctttggttt tttcttgggc tgagcctgca
     6001 aggacaaagc ccccaacggt ttgttctttt tcttctattt ttaagaccac acggtcccct
     6061 aatggtttca acatctgatt tcctccataa tgagatagat attattagca ctctttgaac
     6121 ttgagtgcta attcataatt ctattgtatc acttggtcag aaatagtcaa gaaaaaagtc
     6181 tgactttctc aagataaaaa gcctgagacc aactcagact ttttaatgtt tatactcaat
     6241 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaagcatt gctttgatgt
     6301 tgtggataaa gctaacgtgg tttgaagaga ttttcgaaga gtattaaaat ggcaattctt
     6361 cctcttccaa gaccaaatct gccaaatctt ggcctgcatt attttcacgc atggcacgtt
     6421 gagcacgact ttctaagagt tggaatcctg tcacaaggac ttcggttaca tagttcatct
     6481 gaccattttt ctcaaagcga cgggtacgca attctccatc aacggaaatg agactacctt
     6541 tggttgcgta gcttgccaaa gtttctgcta gcctgcccca taggaccata ttgacaaaat
     6601 cagcttcacg ttcaccgttt tggtctttgt aacgacggtt gacagcgata gttgctcgcg
     6661 ctaccgactt gtcattgttg gttttgtgca attctggtgt agacgttaaa cgcccaatca
     6721 agataacttt attatacata ttttcttcct cctacttatc tattcgtagg aaatcaaaaa
     6781 aagttacaga aatttgtaac ttttcgagga aatttcttat tttttatgaa tcatgaaacc
     6841 tgtcgcctgt tgattggcca taatggtcat atctgtaatc tgaacacggc gaggctgact
     6901 ggtcacatag actactgtgt ctgcaatatc ctgagcttgc aaggcttcta ttccttggta
     6961 aacggacgca gcccgctctt tatcaccatg aaaacgaaca gttgagaaat ctgtttcgac
     7021 aatcccaggc tgaatggttg tcaccttgat atccgttgcg atggtatcaa ttcgcagtcc
     7081 atccgaaaag gtcttaaccg ctgccttggt agctgagtaa acagcggcac cagcataggc
     7141 gtagattcct gcggttgacc ccatattgat aacgtgacct tgattgactt ttatcattgc
     7201 tggcaagaaa cagcgagtaa ccgccatcag acctttaaca ttggtatcca acatggtcaa
     7261 catatccaac tcctcatagt cttgataggg agccaagtca agagccagtc cggcgttgtt
     7321 gaccaagata tcaatcctcc ctatcgtttc tagaatatca gagcaaacag tcttcaccat
     7381 gaccatatcc gtcacatcta gtggaaaagt ccagacagtt tgatttggaa agcttactgc
     7441 aaactccgac ttgaggattt ctaatctgtc tatccgtcgt cctgttagaa cgacatcctc
     7501 accctgctcc agataagcac gcgcaatcgc ttcaccgatt cctgaggttg ctcccgtaat
     7561 cactacattt tttgccatct tatttccttc tatcagatgc tgacaagttc ctaggcagtc
     7621 cagtgtttcg ctgggtcgaa cggtgttccg acaacttggt cttctgataa ttcaagcacc
     7681 ccacgttttt gcggagcatt tggcagatgc aattcacgag gactgcacat cataccaaaa
     7741 ctcttttcac cacgaagttc acctgggaaa atgagattcc cttttggcat catagctcca
     7801 ggaagcgcga caatggtttt caaccccaca cgcgcattgg gagctcctgc aacgatttgt
     7861 acagtcttat cacttgcgac tgcaacttgg cagatgttga ggtggtcact atctggatgg
     7921 gctaccatct caacaatttc accgacaaca aacttaggtt ccttatcatt aacaatttct
     7981 tctgtaaaac cttccgcctg caactcttgg ttcaaacgag cgacttgctc atctgtcaaa
     8041 aagacttgac cgcgctctgc aatttcaaat aaacttgaaa cttcgaaaat attccaagcc
     8101 actgtttccc cattatcttt gagaaaaaca cgggctacct tgcctttgcg ctccacatcc
     8161 agtttggcat ctccgctatt tttcacgatg accataagga catcaccgac atattctttg
     8221 ttatacgtaa aaatcattgt ttcctttttc tcctatttca atcctgctaa aaaatcattg
     8281 atttgttgct tgcttttacg gtcgcgattg acaaaacgac cgatttcctt gtccttttct
     8341 agaacaacaa ggctaggaat tccgtaaaca tcccagagtt tggccaaatc catatactga
     8401 tctcggtcca ttcgaataaa ggtgaactct ggattggtct cctcaatctc tggtaaggca
     8461 ggataaatat aacgacaatc gccacaccag tctgccacaa aaaggaagac cttcttgccc
     8521 gctttttcca ctaaagatgc taattcttct aaacttgctg gctgtatcat aagacttcct
     8581 cctcatagac taggtcttca ttttcataga taaaggtata atgacggcca tcctcaaaaa
     8641 tgacgccacc aaccaagctc tccagactgc tttcgtaaac ttgaacatag agggtcgcaa
     8701 tttcccccat gtcggaaaaa aggtctcgca caatctctgt caactcttcc tgagtcttca
     8761 tgagcttacg gtcatctgca acttttttcg tagcaagagc aaggcttccg atacctagca
     8821 gagccaagcc tgccatccac atttttttag ctttcatacc attcatttta acacaaaaaa
     8881 ggcttcagga caaatgagga agcagcagaa aagcaagtaa aaagcctctt cctttaagga
     8941 aaaagacttc ttatactaag agcaaactag gaagctagcc gcaggctgct caaaacacgg
     9001 ttttgaggtt gtagataagg cgacgttgac gtggtttgaa gagattttca aagagtatta
     9061 ttcttattgc caggcaccta agttgccaac gtagtaacta tcaggtgtgt aggtattgcg
     9121 aagcatctta cctgatgaag ccagataata ctacttgcca ttgtctttga cccaatcatt
     9181 cgcaatcatg gaaccagaag aacttacata ataccattct cccttgtcat aaacccaagt
     9241 actgactttc atggttcctg agcaattaaa ggcaaaaaaa ctgtccaata tcattcgttt
     9301 tttaaaagca tttgacacta catcatctac gctgtagtac atagatacat caaacgtaaa
     9361 agccaaaata tcgaaaaata tacaactttt aaaggcagca tttcattttt taaagtctaa
     9421 caaccataga actgaaatca gagcaaggca tcaatttcct actcttagta cgtctatttt
     9481 gatggttgaa gtgaagattt ttttatttaa cgtagacaat ttccaacttc ccaaaagcca
     9541 catctccacg gatagtcaag gttttgctgg ttggggctac aggagcgtca gccttagctg
     9601 caccaaagga ggtttctact ttcaaatcta cacgccagtg ttgtggaaca tagacggttg
     9661 cattcccgaa ggccacttca atatttaaag ttgcaaaatc acctagcatc tctgcattat
     9721 catagtagat tttagcatcc ccaaaagcga cttccacttg gtcatctacg agatcttgat
     9781 cttgcttata gaaggtcccg ctaccaaaag cgacttcctt atccgtgacg actgtttttt
     9841 tcccattgta ccaccatttt ttttcattcc agaacttact tgaatgcgtc agataaccaa
     9901 caccaagtac caccaagatg ctagcccaaa taagagaatg attggtaact ggtaacaagt
     9961 cataagcgta atttgcaatg atgagcgcca gtaaacctgt aaaaactgcc gaagtgagat
    10021 gacgtctaag gatggactca atggacttat aagcaaaaaa aacaatacct agtaaaggcc
    10081 atattttacc atccaaagaa ggaattccaa aattcccttg cagcaagatc caagctgcta
    10141 aaaccaacaa aacaatacca aatgctttct ttttcatgtt ttttacctca tgtttcttag
    10201 attttctttt aggagggatt ggtaatgccg tgagacatga acctccttgt gcgtctgata
    10261 aaaggaaatg gtgcccgttc ctgaaaagga cttgtccact gagtaaatct gacggatgtt
    10321 tgcgatcgtt gacttggaaa ctcgattaaa atagcgaggc aagatagact ccaactcata
    10381 gagcttgagg cgaacctcat aagcttcctt ctggttatga gcgtagatct tgctcccttc
    10441 tgtttcaaag aagagaattt ctgacaggtc tagataatat tcacctgtcc ccttgtaaaa
    10501 agtcaacctc ggagcctttg actcttgcaa gagccgttgc aagtctgcaa tttcatctgt
    10561 caaagtgggg gtcttgatga caatttcagt ctcctctaaa ttgccgtcaa tctcaattcg
    10621 taacttcatc acatctcctt tctgactcta ctatatcaaa gctaagttag gaatacaagg
    10681 gcaaaaaagc aagtggtcaa tttgaagcag taagtggttg taggactagc ttaacttaca
    10741 tgtaagaata ggaagaaaag gaatccccac accaaaccac aagcatagcc aaccaagaca
    10801 tccttgggat aatgaacacc ccctaaaacc ctcaccaacc ccagcaaagc tgaaaagatc
    10861 aagcatacta agccaatagg tagactagca tgcagacagg ccatggagat gatagttgcc
    10921 gaaaagacat ggcgactggg catcgaccgt cccgaactat ccttatcaag aaggggacag
    10981 atatcccaag tttcataagg tctcgggtga ttgattttct tacgaaacag ggacaaggcc
    11041 acaaaacctg atgcaggaat aagcacacaa accccaactt gctgaccaag tcctagttgc
    11101 aggtaagtgg tgactagcaa gatcagatag accaggggca tggcgactgt catcaaacga
    11161 ttgaaactgc gtaataggaa taaaagaatt ggacgactag ttagtctgga gctgatattt
    11221 cgataccatt cttgataatt tttcatttat gcttctcata cttttctaga acttgttgag
    11281 atgttttccc cttggcaagc caatccacta gcttgtccaa gcgacgtatt tcctgcatca
    11341 gaggatcatc aatttcctca atacggacac tgcaaatctt ccctgtaatt gcaattcgtt
    11401 cagggacata atatggtgct tgtctaaaaa aatctccgta agtaacatcc ctatccagac
    11461 aagcaagaac atcactcact tcataccctg tcaaccaact ggtcacttgg tgaactgact
    11521 ctgcctttcc accctttcgc tcaacttttg caatcaaggc taagtagaca gcagcaaatt
    11581 tcatattgta taatttctga ctcatacttc tctctttcta tgtgtactag tataaataag
    11641 aaaaagaagg ccgtcaagcc ttcttttgat ttattcttct gcttcatctt ctgtaaattg
    11701 actattgtac aagtcagcgt agaagccacc ttgcgccatc agttcctcat agttgccttg
    11761 ctcgatgata tttccatctt tcatgaccaa gatcaagtct gcatttcgga tggttgacaa
    11821 gcggtgggca atgacaaagg atgtgcgtcc ttccatcaaa cggtccatgg ctttttggat
    11881 caattcctct gtccgtgtgt caacagaaga agtcgcctca tccaaaatca aaagcggtgc
    11941 atccttaaga agggcacgag caatagtcaa tagttgtttt tgtcctacag acaaggtcac
    12001 ggtgtcatcc aagatggtat catagccatc tggcaaggtc ataataaagt ggtgaattcc
    12061 cacagcctta ctagcttcca tcattcgttc atcactaatc cctatttgat tatagatgag
    12121 attgtctcga atagttcctt caaagagcca ggtatcctgc aagaccattg aaaaggcatc
    12181 atgcacttct gaacgcgtca tagccttggt atccacacca tcaatgtgaa tacttccctt
    12241 atcaatctca tagaatttca tcaaaagatt gacaatggtt gtcttaccag tcccagtcgg
    12301 cccaacaatg gcaacctttt gaccagcatg aactgtcgca gagaagtcat agtcttgaac
    12361 attgacaccg tccaccagaa tttctcctgc tgacacgtcg tagaaacgtg gaatcagatt
    12421 gaccagagtt gatttaccag aacctgttga cccaataaag gccactgttt gaccagtttc
    12481 tgctttaaag ctaacatgtt caataactgc ctccgaattt gccgcatagc ggaaggtcac
    12541 atccttaaac tcgacctgac ctttgaagtt ttcatcagtc agctgcactt gaacagggtt
    12601 ttggatagaa gaatgcaaat ctaaaacttg attaatccgc ttagcagaga ccatagtttg
    12661 gggaagaacg atgaagagtg ctcccatgag aaggaagtcc atgacaacct acatggcata
    12721 agacatgaaa acaatcatgt cactaaagag aggcagacgc gctatcggag cagcgtcgtt
    12781 aatcacatag gccccaatcc agtaaatcgc cacactcaaa ccacttgaaa tccccatcat
    12841 gataggattc aaaatagcca taagacggtt gacaaacaaa tttaaacggg tcaattcatc
    12901 atttactgct gcaaattttt cattttgata atcctctgca ttgtaggcac gaacgacacg
    12961 aatacctgtt aaactctcac gagtgatact gttcagttta tctgtcagcc cctgaatcaa
    13021 ggactgtttt ggaaaggcta gcgtcatcaa aacggtcgtc atcaggacgt tgacaatcac
    13081 tgccacaagt acggcccaga gccagtattc tgaatgacct aaaatcttcc caatagccca
    13141 gatagccata attggaccac acgttaccac ttgcaagtcc atagtaatca acatttgaac
    13201 ttgagtaatg tcattggtag tacgcatcaa gaggctagga attgaaaatt tcttaatctc
    13261 tgtctgcgag taatccaaaa ctcggttaaa aatatcactt ctcagcctac tagtataaga
    13321 agccgccact cgggatgcaa aaaatccaac tgcaactacg gacaagaagg caagaaaggg
    13381 cattcccatc atcatgcttg ccggctgcca caactcatct aaattagttc cttgactacc
    13441 tagcaaatcc gtaattttcg agatataggt cggcacttcc aactctagat agaccgaaaa
    13501 gcaagtaaag agaatggcta gtaaaatcat cccccattct tttctactaa ttcttttgac
    13561 taatttcttt attctctcct cctattccct tgatatttgc ctgtagttga ccgagaactt
    13621 tctcaaaaat cagtaattca tcttcatcaa tgtcttccat caactgcttg tctatgcgtt
    13681 caaaaaaagc cttaacctgt tgcatctgag aacgtgcttt gtccgtcaga cgaacaaact
    13741 tagcccgctt atcaacagga ctcgcctcca attccaccaa accattttgc actatacgct
    13801 taaccagatt actagcaaca gacttggtaa tattgagttc ctgctcgata tctttaatca
    13861 agaccaagtc ttggtttttc tcgcgattat ccaaaaaatg cacaacctga ccttgcggcc
    13921 cacccataaa ttcaatgccg caacgtttgg cttccttttg caccatcagg tgaatttgat
    13981 gaccaaaacg cttaaagact aacatcggtt tatccataat ctcccccttc taaataaaaa
    14041 tagttctctg gagaataatt aaatttctat gagaactatt ttcttgatta aaaaaatccc
    14101 aagtgatttt ctcacttagg atcatgttct ataggttaaa ttaaaaccca tctacgttcg
    14161 tataaatctt ttggacgtct tcgtcgtctt caagaacgct gtaaagtttt tcaaaggttt
    14221 caaggtcttc gcctgacaat tccacttctg actgaggaat catttccaat tcagtcactt
    14281 ggaattcttc aataccagac tcacggaggg caacgatagc cttgtgaagg tcagttggcg
    14341 ctgtgtaaac tgtgattgta ccttcttgtg cttctacgtc atccacatcc acatccgctt
    14401 cgagcaattg ctcaaagact gcgtccgcat cttcacctcc aaatacaata acacctttgt
    14461 tgtcaaagag gtaagaaaca gaacctgaag cgcccatgtt tccgccgttt ttaccaaagg
    14521 ctgcacggac attggctgct gtacggttga cgttagaagt caaagtatcc acaattagca
    14581 tagagccatt tggcccaaaa ccttcgtaac gtccttctgt aaaggtttcg tctgtgtttc
    14641 ctttggcttt atcaatcgct ttatcgataa tgtgttttgg cacttgggct tgtttagcac
    14701 ggtcgataac gaatttcaaa gctgagtttg attctggatc tggatcacct tttttagctg
    14761 ctacatagat ttctacacca aattttgcat atactttaga gttggctcca tctttagccg
    14821 ttttcttggc tacgatattg gcccatttac gtcccattag gaatctcctt ttttcacatt
    14881 ttaatctttc ttattataac acaagttttt ttgattttca ctagaggaaa tggattttat
    14941 tagcaaatca agctaggata gcactttacc tgctaagatg gtcttgcctt tctatcttta
    15001 tcaacagaca ctcatccaca ttcaaaaaac aaactagacc attatctgca aatagaaagt
    15061 ttcagccaag tttgacaaag tcagctcaaa ttactgtttg aagtttgtag atataagcga
    15121 caaaaacaat catactgcac cttttgttga cagtctactc cagacatatc atagttcaag
    15181 taaatacttt gaaatccaac agttcttata ggcgctattg tattctaaga aatcaataga
    15241 agagtttcta agcaaacctc taatactcaa taaaaatcaa agagcaaact agaaagctag
    15301 ccgcaggttg ctcaaaacac tgttttgagg ttgcggatag ggctgacatg gtttgaagag
    15361 attttcgaag agtataattt acgtgttcct aagatggaga agttagacta gtacactggc
    15421 acttctaaac cattgctagc aattgatttg ttcatattta atttcatttt ttccataaat
    15481 gggtattaga tataaacagc aaaatatttc cgatacgtgt cattcttgaa tttccaatca
    15541 tctaaaacaa gtaaaggata atcaatcccc tgtatatcaa ggaattggct acccttttta
    15601 cttttttaca catcctgttt gatagactca ttttaacatc acgagcatac tccaatggaa
    15661 atcgctaggc aagagataaa ctttcagata ttcgcagaga gctcatcgcc tctttttgtc
    15721 gcaaacattc ttctctccta atcattttct accttatcct ctacctgagg atagagagtt
    15781 gttccccaaa tagaaatcgt ccgcttacgc actagtggca aatcggtttt ttcataaacc
    15841 gtacgccacc attcccaggc aagcccggta cactctctaa ttttgacaga gagattgcga
    15901 acattccctt ttaaaggaat actagtggta aagtgagccg tcaaatcctg cccatttctg
    15961 tcccaagcct taggagtcaa gacttccttg ccttgatgat cataggataa ttcatcccaa
    16021 gtaatataat attgagcaac ataggcacca ctatgatcca gcagtaaatc tccgtttctg
    16081 taagctgtaa ccttagtctc aacatagtct gtactgtttt gaaaggtcgc aactacattg
    16141 tcacgtaaaa aagaagttgt ataggaaatc ggcaagcctg gatgatcggc tgtaaagcga
    16201 ctgccttctt gaatcaagtc ctctaccata tccaccttgc ctgttacaac tcgggcaccc
    16261 gaacttgggt cgccccctaa aataaccgcc ttcacttctg tattgtccaa aatctgcttc
    16321 cactctgtct gaggagctac tccttttatc aaagattcaa aagcagcctc tacttcatca
    16381 ctcttactcg tggtttccaa cttgagatag acttggcgcc cataagcaac actcgaaata
    16441 tagaccaaag gacgctctgc agaaattcct ctctgcctta aatcctctac cgttacagta
    16501 tcttgaaaca catctcctgg atttttaaca gcatctacgc tgactgtata ataaatctgc
    16561 ttaaaattaa caatctgaat ctgcttttcg cctgaatgga cagagttaaa atcaatatca
    16621 agagaattcc ctatcttttc aaagtcagaa ccaaacttga ccttgagttg ttccatgctg
    16681 tgagccgtga ttttttcatg ctgcattcta gctgggacat tattgacctg accataatct
    16741 tgatgccact tagccaacaa atcgtttacc gctccgcgaa cacttgaatt gctggggtct
    16801 tccacttgga gaaagctatc gctacttgcc aaaccaggca aatcaatact ataagtcatc
    16861 ggagcacgat cgaccgcaag aagagtggga ttattctcta acaaggtctc atccactacg
    16921 agaagtgctc caggatagag gcgactgtcg ttggtagctg ttacagaaat atcacttgta
    16981 tttgtcgaca agctccgctt ctttctttcg ataacaacaa actcatcggg tagctgatta
    17041 ccctctttga tgaaacgatt ttcaatactt tctccctgat gggtcaagag tttcttttta
    17101 tcgtaattca tagctagtat aaagtcattt actgctttat ttgccatctt ctacctccta
    17161 ataagttcct ggattgagtt gcataaactc agacttgttc agcgaaatca gccgtggttg
    17221 gactaagtaa tccaaaattt cctcgtacaa ttcttctgag acattgcgtc gccgtctggc
    17281 taaataagaa gtcggaatga ccgtattatc caacataaat accttatcta agtcaatcaa
    17341 ggttggtctt gtaaaaggat tacgagctag atccggctct tctatcataa agttcttgac
    17401 caaacgtctg gtcaagagag ctggtttgaa ggtctgattt ttaaccaact ctttgttttt
    17461 agtcatgctg ttgtcaatac agatatacat atgattcttc acagccaaat cgctactaat
    17521 agtcggaaaa ggcaaataaa gagctacaac atctcctctc ttaatcaagc aagagcaccc
    17581 ccttttctcc taatgtaaca tagacaggat tgaccaagtc ttctgattga ctcagaattt
    17641 ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc ttgtctcttc aatacaaaat
    17701 gcttgtcgcc aataaccttg acaatataat ccttctccaa agctgactgg taaatccaca
    17761 tcagatgttg tctgtcctga gaactcaaga gagaaggatt ttcaagcctc ccgatagtct
    17821 gataaaaatc aaaaacagga gctaactcct gccaatctga ttggctagtt gtcaaggcta
    17881 gaaaaagggc tttgcgagct gatacttctt ggttagcctt gagagttact ttcccctcca
    17941 agttttttag aaatcgggaa actccagaaa gcaaattttt ctctaactgc gagaaataaa
    18001 aacctttcgt tcccagacat aagtctttca tgtcgctttc tctagcaaat aagagctcaa
    18061 acatttgata gtaaaagaaa aatatctggc actgggtcgc gctcatcttt tccttatcgg
    18121 cttctttttt taaccagagc aagggcgaca ggtagctgga ttgagacatt tcctctacct
    18181 cctactcttt tttaactgga gcatctgcac tagctgccac ttcttttgac tggatacttt
    18241 cccactggtt aatctcctct gagataagac cttcgcatgt cttgacaaat agggcaaaag
    18301 ccttggtctt tcctgcatat ttctccgttt ggcattgata gaggaatttt tctttctcca
    18361 ggagttgcgc agttttttgg taagaaatcc aattttcctt tgcattatac aaattgataa
    18421 tcccctcaca cagcaagccg agactggata aggcaaccga aatcaaacgg tagcgatcac
    18481 ctggcatagg aatagcacaa aagacagcta tgaggaaacc tgccacgatt tctgttattt
    18541 ttaatacctt atagcgccta cgatgttgaa cgcttttctt taaaaaatga gctatctgta
    18601 cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat attcgtaact cctttcattt
    18661 actttgataa ccatagtata acatatttga atatcaaagt atagtaaaaa ctggactata
    18721 acttattaga ctccctgcga aacaaaatat ggtatagtag ttctatgaat tatgaagcaa
    18781 gtaaacaatt aactgataca cgatttaagc gtcttgttgg tgttcagcgt accacttttg
    18841 aagagatgtt agctgtatta aaaacagctt atcaaaaaag tcggacaagc tggtaaaatc
    18901 ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    18961 aaagccagca aactcaagcc gctaacagct gaagataaag cctataacca tgcgctatcc
    19021 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgatttca
    19081 acaacctatc gaaatcatcg taaacgct
//
LOCUS       DBERCA010000037        18437 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.037, whole genome shotgun sequence.
ACCESSION   DBERCA010000037 DBERCA010000000
VERSION     DBERCA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.037"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..800)
                     /gene="lytB"
                     /locus_tag="ACL719_001405"
     CDS             complement(<1..800)
                     /gene="lytB"
                     /locus_tag="ACL719_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HGS0699359.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGK"
     gene            complement(842..1780)
                     /locus_tag="ACL719_001406"
     CDS             complement(842..1780)
                     /locus_tag="ACL719_001406"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGS0699360.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEYAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1791..2591)
                     /locus_tag="ACL719_001407"
     CDS             complement(1791..2591)
                     /locus_tag="ACL719_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGS0699361.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2841..3221)
                     /locus_tag="ACL719_001408"
     CDS             complement(2841..3221)
                     /locus_tag="ACL719_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0699362.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3279..3638)
                     /gene="rplT"
                     /locus_tag="ACL719_001409"
     CDS             complement(3279..3638)
                     /gene="rplT"
                     /locus_tag="ACL719_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGS0699363.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3690..3890)
                     /gene="rpmI"
                     /locus_tag="ACL719_001410"
     CDS             complement(3690..3890)
                     /gene="rpmI"
                     /locus_tag="ACL719_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGS0699364.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3923..4453)
                     /gene="infC"
                     /locus_tag="ACL719_001411"
     CDS             complement(3923..4453)
                     /gene="infC"
                     /locus_tag="ACL719_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGS0699365.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4469..4600)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4760..5941)
                     /locus_tag="ACL719_001412"
     CDS             complement(4760..5941)
                     /locus_tag="ACL719_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699366.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYFNSQTANSVS
                     LERELETRLVDRERVINEYEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKDYEFVSNDPTASSELKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEAIIPTLFVIGIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYSIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     gene            complement(5945..6532)
                     /locus_tag="ACL719_001413"
     CDS             complement(5945..6532)
                     /locus_tag="ACL719_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699367.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6565..6782)
                     /locus_tag="ACL719_001414"
                     /pseudo
     CDS             complement(6565..6782)
                     /locus_tag="ACL719_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6931..9171)
                     /locus_tag="ACL719_001415"
     CDS             complement(6931..9171)
                     /locus_tag="ACL719_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGS0699368.1"
                     /translation="MLQWIKNIPIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLIKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSLRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPTFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(9155..9805)
                     /locus_tag="ACL719_001416"
     CDS             complement(9155..9805)
                     /locus_tag="ACL719_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS0699369.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(9873..10442)
                     /locus_tag="ACL719_001417"
     CDS             complement(9873..10442)
                     /locus_tag="ACL719_001417"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699370.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10619..11730
                     /gene="ald"
                     /locus_tag="ACL719_001418"
                     /pseudo
     CDS             10619..11730
                     /gene="ald"
                     /locus_tag="ACL719_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(12031..12357)
                     /locus_tag="ACL719_001419"
                     /pseudo
     CDS             complement(12031..12357)
                     /locus_tag="ACL719_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12360..12860)
                     /locus_tag="ACL719_001420"
     CDS             complement(12360..12860)
                     /locus_tag="ACL719_001420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699371.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            complement(13053..14021)
                     /locus_tag="ACL719_001421"
     CDS             complement(13053..14021)
                     /locus_tag="ACL719_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGS0699372.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14107..14322)
                     /locus_tag="ACL719_001422"
     CDS             complement(14107..14322)
                     /locus_tag="ACL719_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGS0699373.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14331..15185)
                     /gene="cvfB"
                     /locus_tag="ACL719_001423"
     CDS             complement(14331..15185)
                     /gene="cvfB"
                     /locus_tag="ACL719_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGS0699374.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(15245..15802)
                     /gene="frr"
                     /locus_tag="ACL719_001424"
     CDS             complement(15245..15802)
                     /gene="frr"
                     /locus_tag="ACL719_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGS0699375.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15811..16548)
                     /gene="pyrH"
                     /locus_tag="ACL719_001425"
     CDS             complement(15811..16548)
                     /gene="pyrH"
                     /locus_tag="ACL719_001425"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGS0699376.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHLDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16632..17966)
                     /gene="trmFO"
                     /locus_tag="ACL719_001426"
     CDS             complement(16632..17966)
                     /gene="trmFO"
                     /locus_tag="ACL719_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGS0699377.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(18211..>18437)
                     /locus_tag="ACL719_001427"
     CDS             complement(18211..>18437)
                     /locus_tag="ACL719_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS0699378.1"
                     /translation="SQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHEDSQFKGDHYV
                     ECYAVLDNTVIARDRITVPIDPLCGKDFIE"
BASE COUNT         5325 a         4173 c         3395 g         5544 t
ORIGIN      
        1 attttcccat caaatttgag ataaaaccaa gattccttat cccaaatcca ttcattggct
       61 gccatgtagc caccggattt tagataataa tagtgaccat tctcgaaaat ccattcttta
      121 tcagcatagt ttccattttc tttgatgtaa aaccaagatt ggtattgttt gtcaaaaagc
      181 caaccttgct gtactttggc accactagca ttcacataag cttgattaat ccactgactt
      241 gtcagtagat aaccaccgga tttgaaataa tagtccttcc ctttaatttg gagccattct
      301 ttctctgcgt gctgtccatc tgctttgata taaaaccaag catcgtattg agaatcatag
      361 acccagtctt ctattacttt ggcacctgtt gcaccaacat aggaagttcc tacccaagca
      421 tttcttttca tctttccatc ttggtcaaga taataaaagg ctcccttgtc ttctacccat
      481 tctgatttgg ccatatagcc accagatttg aggtaaaaat agtcgtcacc ttgctttagc
      541 cattcatttt cagcatagtt agcatctgct tttatataga accaagattg ataatgagta
      601 tcaaaaaccc actcatttgc tgcttgactg ccatcttctt tcagatactg ttttccttgc
      661 caagtaccat cactagccat tgcaccctct ggactagcta agaaaaatag cagagctaaa
      721 aaaataaatc ttactttctt cattaacctt cttcctctgt tcttatttat tttattgtac
      781 caaaattctc ctaaattcat attacaaact aataaaaaat caagaacaga ttgattgcag
      841 tttacctcag agactctttt acttcctgac ggagttcttc cagactgcta ataccgtatt
      901 tatccatgac ttttggtaaa ttttcgatga tgtcagggca ggcataagga ttggtaaagt
      961 tagctgttcc aactccgata gcagatgccc cagccagata catttctagg gcagcttcag
     1021 ccgaatccac tccccccatt ccaatgatag gcaggtctgt tgtttgggct acttggcgga
     1081 tgagtttgag ggctactgga aagactgctg gacctgacat tccacctgtt ccattggcca
     1141 agattggttt tctggttttg aggtcaaagc gcattccaac cagagtattg atcatggtca
     1201 agccacttgc tcccgcatat tctgcagctt ttgcgacagt aacgatatcg gtcacactcg
     1261 gggttaattt gacataaact ggcacttctg aggcttccac agctgctttc accacatcat
     1321 aagccaaatc tggatcttga ccaatcaaaa gtccatgatt acagtggtca acattgggac
     1381 aagaaatatt gagctcgata gcttttacat tagttgcctt ggaaatccca tgagaaacag
     1441 ctgcatactc ttgttttgaa aaaccagcta cattggcaat aataggaaga tttggatatt
     1501 ctctttccag ccaaggtagc ttttcagcca aaacaacctc taaaccagga ttttgcaagc
     1561 caattgcatt gagcatacca gcaggcgtct ctgccactct tggagttgga ttcccaaaac
     1621 gtggttcaag ggttgtcgcc ttgatcataa tagaacctaa aaggtctaaa tcatagtact
     1681 tggcatactc ttgtccaaag ccaaaacagc ctgatgctgg aataatcgga tttttcaaat
     1741 ccaaaccagg tagagacact tgtaatcgat ttgtagtcat aattttctcc ttataataca
     1801 actgttcctg tgcggaaaac aggaccatct tcacagacgc gttggctgac cgtctcgctt
     1861 tctggtactt ttagaacaca ggcatagcaa gctcccatcc cacaagccat acgagattcc
     1921 agagataaat aggctcttgg gtgatcatca aaggtttgat tgatatactt catcattcct
     1981 ggagccccac acgagtaaac agcatcaaac tgactatcta aatcattgat aacaacggaa
     2041 acatttccct tgatgccata agaaccatca tctgtcgtta caaagacctg accatactga
     2101 gccaattccg ttttcaaaat aacagcatcc ttattagcaa aaccgaggac tgtcactact
     2161 ttcactccac gttcatgcaa ttccttggcc acctcaagca agggtggaac accaatccca
     2221 ccaccaacaa ggagaacctg attctgctca tcaaggtcag acaagtcaaa accatttccc
     2281 tgaggcccca tcacatcaag agtgtctccc tgacttaagg ttgaaaaaat tgcagtccca
     2341 gctccctcaa tccgataaat gaggtgacac tgcttgtttg ccttgtcaat agacgaaatt
     2401 gaaataggac gacgtaagag atgggcatcg tctggtacac gcagatgaag aaattggcct
     2461 gctcgcatgg cttcaaccat ttctccttct aggactaatt caaagattgc tggcgcgatt
     2521 tcctcttgtg caaccacctt catggtttcc aaccgaatga cacccaaacg cttcttacat
     2581 gtgagattca taacttttcc tccttaaatt ttaaggggac cagataaaga aaagaccttg
     2641 ctagtgcaag gcctcagtta aaatcgtaca acacaagaga gcacactcaa aaggtctcct
     2701 ctagaaaatt gcactattct tttatctgac accttgtgag tctctctgga ctccccttaa
     2761 aggttgaatt tttattagta tactctttca gctaaaaaaa gtcaagtaga aaacgaatat
     2821 tctacttaac ttgcacgaaa ttatttttca cgaatgactt cgaccttata tccatcaggg
     2881 tctttaacaa agtaatagtt tggtgtagtt cctggtagac catttggctc agtcacttca
     2941 tagccttttg cactgtgttc ttgatgaagt gcctcaagat caggtgtact gagggcgata
     3001 tgggcaaacc catcaccaac cacatacgga ccgtgatcgt agttataagt caactccaac
     3061 tcataatcat caccctcaag acctagatag acaatcgtga aggcatggtc tggaaaatct
     3121 ctgcgacgca attctttaaa accaaaagca tcttgataaa atgcgattga tttttcaaga
     3181 ttttctactc gtaagcaagt gtgtagcatt tttgaagcca tatctttctc ctttattttt
     3241 aaaaagactg gacaatcctg ttccagtctc atcagttgtt atttaccaag ttttgcttta
     3301 gctgcatctg caagagctgt gaaagctact gcatcgttaa cagccaagtc agcaagcatt
     3361 ttacggttaa cttcgatctc agccaatttc aaaccatgca tcaattgtga gtatgaaagt
     3421 ccgttcatac gagctgccgc attgatacga gtgatccaca atttgcggaa gtcacgtttt
     3481 ttctgacgac ggtcacggta tgcatagtag taagagttca ttacttgttc ttttgcagta
     3541 cggaacaaga tgtgtttagc tccatagtaa ccttttgcta atttaagaat acgtttacga
     3601 cgtttgcgtg atacaacgcc acctttaaca cgtgccatgt tttatttcct ccaaatattt
     3661 cctagaattg tttacttaca gtcaagctat tatttcaagc gagtaagcat tgctttgata
     3721 cgtttgtaat ctcctgaatg caccatagat gctttacgaa gatgacgacg ttgtttctta
     3781 gtttttccgt ggaaacggtg agaagtgtaa gcacggaaac gtttaagtcc accagaacct
     3841 gtacgtttga aacgtttagc tgatgcgcgg tgtgtttttt gttttggcat gattttttct
     3901 cctttattta actttctgac aattattttt tgtcagtcgc tggcgccaat tgcatgaaca
     3961 tttgacgtcc atccatttta gcacgttgtt cgatgatggc aatatcttga gttgcttcag
     4021 caaactcggc taaaactttt gcaccaatct ctttatgggt aatcatacga cccttaaagc
     4081 gaatagatac cttaacttta tttccttttt caaggaactt acgtgcatta cgaagttttg
     4141 tatcaaagtc acccttgtca attgttggac ttagacgaac ttctttcaca gtaacaacgc
     4201 tttgtttttt acgttgttct ttttgcttct tctggtactc aaatttgaac ttaccgtagt
     4261 ccataatttt tgcaacaggc ggtttggctt ggggttgaat caatactagg tcaacattag
     4321 cgttatcagc caaagcttgc gcttcactga gtggcttgat gcctagctgt tctccctcaa
     4381 gaccaatcaa gcgaacttca cgtacacgaa tctcatcatt gatgaataag tcttgctttg
     4441 ctatggtttt cacctctttt gttttattag agaaaaacaa tagcggactc gtaaatatac
     4501 aagcccgcac gttatgatag cgtttcttcg aaacttttca tccgtagggc caggcaactt
     4561 aatgtcacaa ggcgagaagc tctcacttct gcttttctca acttttatat gataccaagt
     4621 tttctagctc ttgtcaagat aaaaagttat tttttgaaaa gtttgtgttt gtattttggt
     4681 ttggttttcg aatcaaaaag agagaaccaa gttgattctc tccctatgtt agtttttctt
     4741 tagcttccct ataggaaaac taaactttaa aaacttcctt tttacgtgaa cttccccagc
     4801 tttcgataaa tagaatcccc actaacaaaa ggataatcag gcaaggaagt aagaccatcc
     4861 ccatgctcca atttagatcg acattatcaa tctgcttaga taatcttcca gataaaatct
     4921 ccactgaacg caagtaagta aagggaatca gatgtgcaat cctttgaaga ggctgaattg
     4981 tttggatgcc aaacaataag ccaacaatcc caatgagtga aagaaagagg acaggcattt
     5041 tttgcttgaa aaaataagca atcaagtaca caacttccac aatgacgata aaggctaaga
     5101 aagctaagag taagccagga aataatacat cttgtatttt cccaatagtt acttcttgat
     5161 tcactaagct ataaattgag tagggataat ctaactgtcc aaaaccactt atcagacttc
     5221 ccactagaaa agaaaagcca cagattccga taaacagcac agttacatat cccactccaa
     5281 ctccaagaga ggatattgca aatgtcactt ttgaaacagg atataagtga gctgtgtcca
     5341 gatgattttg atatctttct gcaaatagtt gtgttagcat aaaaataata ccaatcacaa
     5401 ataaggttgg gatgatagcc tctaaaatcc agacaatctg atcaatcccg tgggtcggaa
     5461 actccaaagt atgtgctttt atgttcaagg gatacagggc ttgataaatc ttccgttcgc
     5521 ggtcaacccc catttttaat tcagagctag cagtcgggtc atttgataca aattcataat
     5581 ctttctcttc atcttgccac tgcagatagt aggcctcttt ccagcgccct tcttttagta
     5641 aagttagaat ttctgtcttt cgcgtcaaaa gatttttttg cacgtctaaa ttatttttag
     5701 caaactggta ttcctccgag ctggtatcag acatttggga gagtttctct tcatattcat
     5761 tgatgactct ctcacggtct acaagacgag tttccaactc tctctccaag ctgactgagt
     5821 ttgcagtctg actattaaaa taaaaagtaa caccgagtac agatacaaat aaagctaaga
     5881 taatccagtt taagcggctt ttgaaaactt ttttcaataa aaatagacta acatctttca
     5941 taaactaaac ctcttctatc tgcccctgat gaatggttac tactctatcg cagatatcaa
     6001 ccaactcttc cttatagtgg gaacttaaaa gaaccagctg ttcttgtcta tcgatttgtg
     6061 ctaacctatc aaaaaacttc tgtcgataat actcatctaa gccatttgta atctcatcca
     6121 tgagccagca tttggcctga ctgagaaaat acatagcaat caccaagcgt tgcttcatgc
     6181 ctaaggaata cttgcggatg ggaagactga tatagtcagc catttcccag taggcgattt
     6241 catctctcaa gtttaggtct gacttccaga tgtttttgat aagacgaagg tagtccatcc
     6301 cacttaagtt tccatccagc cattcaatac tctcataata aaataaagaa ggaggagctg
     6361 cgatatttcc actacttatg ggaattaaat tgctaatggc acggaacagg gtcgtctttc
     6421 cagagccatt gatagcaaga agaccataaa acctaccctt tttaaaggtg aaatccgcat
     6481 cttgcaagat gacttgtcgc gtttttaagg tcacatgagt aagctgcaac atatctagcc
     6541 ctcctttttc tcactcttta agaattaata gcctccgctg taatagttta tgatctcata
     6601 acgaccgtag ttccagccag aaccaactgg atactcagaa tagctgtaat aacgagacca
     6661 tgacggaacg ttagtatgtc tatatccatg gaaattcata ctagacttat agaagatatt
     6721 ccaatcatac cgatactttt aagaacactc ttagcagata cactggactg aagaagacca
     6781 atagattata aactaactaa taaaataact tttgctgatt tttaatgatt ttatatcctc
     6841 aatattgaat acgttttcat tataccattt ttatcattat tatgcaacaa atttttagtt
     6901 tatttcacta atctacaaca tttatctttc ctatcgaaca ctttcgattt tccaactatc
     6961 caacccctta aaacgtatag ctccttgctg gtcagttcga taaactttgc tattgatacc
     7021 ttccagtcgt gtcaatgttt cctgatgggg gagtttcatt cgattgctct ttccaactga
     7081 gataagagta agctctggtt tgagtttttc tagaaaggtt ggacttgatg attttttatt
     7141 gccatgttgg ctagctttca aaacatttac tttcaagtct ggatagtgct tcagcaagtc
     7201 cttctctcct ttctcctcca aatttcccgt gaagagaaat tgcttatcca agaatttccc
     7261 atacagaact agggtatcat cgtgtcctcc atctcccatt ttccttagag atagaacttc
     7321 taactgactt ccaaaaatgg gcaagttctc ccctactgtc atactacgca cctttgtttg
     7381 agtcgcctgt agttctacca caaattgctt ctgcttcaaa ctgccttttg atactaaaat
     7441 ctcccctaca tggaaagcct tggtcacctc taacaaatct ccgacatgtt ccttgtccgt
     7501 atttgtcaaa atcagctggt caatcttagc tactcctcga cttttaagat agggtatcaa
     7561 gcttctctgt gcattgctgg tcgtcatctt ttcttgccat ttttcgattt tcttataaga
     7621 ttctgcctta ccacctacat ctatgagaat ggttttccca gttacatccc gtaggaaaat
     7681 actttctcct tgccccacat ccagcatggt gatttcattt tccagtggat acttgataag
     7741 gaaaaagaga cctgtaatca ataaactcaa tactgttaat cctttaatgt ttttcctcaa
     7801 atcatagacc aaagccaagg aaattaacaa taagattaaa agccatgcgt tgggttgacc
     7861 aaagacaagc ggtctccttg ccacctgcga gaccaagcga ataatgccct ctaaccattc
     7921 aaagataaag ttcagctgaa tgactggata gagaaaggaa aggacaaata aaatagacaa
     7981 gagcggtaag aaggtcaagt caaatagaaa ggaaaagaca aaggtcaaaa ggatagacca
     8041 aggttgaaat tccgcaaaat agaaggatag aatgggcaat atgcccaagg agatgactag
     8101 actttcacta gtaacagcct tgagcccctc cccttctttg ctggtcatgg tcaggataaa
     8161 agcataagcg caggacaaga ctcctcctgc tgtcaagaaa aagtttggca tgacaataaa
     8221 gaggacaagc accgtcaagg caaaattatc caagccctta accccatgtt gagccagtag
     8281 cttttgcaag agactgcgaa taaccgatgc tgaaaatcca gttagtcccg catagataag
     8341 ggaaaaggga taagtcagcc atttcaactt ttcttgggtc aagcccaatc gcaagagaag
     8401 tttcttaaat ccattcatga aaaaacctac ctgcatgcca gatagggcaa agaggtggat
     8461 aattcctaga ctggaataaa gctcattcat ctcctcaaag tcggtgtcca gatgtcccag
     8521 caagagtcct gtcatgtaat tgcgcatagg gtctggaaag tgcgtcttaa tccaaaccac
     8581 agcctttcga cgtaaactgg acaagttttc tcctatatcc caactgccaa tcttttgaag
     8641 tgactggatt gttttgatat tgagagtctg gtaaattccc tgagtcttca gataggcttg
     8701 gtaattaaag ccaccaaaat ttctctgccc ttctggctcc gaaagcttcc cttctagtcc
     8761 tatctcatgc aggtcggtta aagcttgaaa ggcttctttc tcctcctcgg actggagttt
     8821 ataatagact tggaaagcac gaccgttaga cttgccacga aaggatagac tatcaccatt
     8881 aaccttaata gtatcaggca aaatccgtac cccttcaaca gaatccgcca gattttgact
     8941 cgcttgactc tgttgccaat tttgaaaaac aaaccaaaat ccaaagattc cacaaatcac
     9001 tagaactttg ctagcagatt tccaaggaaa ttggaaaaag agacagacta gcagaaaaac
     9061 aaagcccaac aaagcaagat aggatgctga gaaaatagcg taataaagcc aaagtaacaa
     9121 aaaactcagg taaattaggg gaatggggat gttcttaatc cactgtaaca tagtctttta
     9181 gcttttctat ggtcttagca ccaatgccag agaccttctt gagttcatca atcgacttga
     9241 atttcccatt tgcctcacga tggtctataa tgtcctgagc tcgttttcct cccagtccct
     9301 taacctgttt aagctcttcc agactggcct tgttgagatt gaccttcttt tccttgcttg
     9361 ttgaagaagc tgtccccgaa ccagtctgtt gactaactgc ttcttctccc ttagtaggaa
     9421 cgtaaaccag agcctcatca ctaactttct gagctagatt gagcgacttg ctgtctgctt
     9481 gctctgtcaa gccaccagcc ttctgaacag catcattgac tcgactacct acaggcaagt
     9541 cataaatccc tggcgatttg acagcacctt tgacatctac tgtgattaga tcttgttcaa
     9601 ggggttcttc cttctcttcc ttgttcactt ccttttcgga taccaaatcc ttggaaacag
     9661 ctgcaacttc agcctgcaaa ttcgtttcct ttacaggtgt ttgtggagct ggctttagca
     9721 ggaaaaatcc gcctacaagc aagcccagac cagtacagat gacgatgatt ttatactctt
     9781 tgattttctc gataattgct tccatatttt ctcctctctt agattattcg taagaggaag
     9841 aaaaaacagt cgaaaaattt cttttcaact gcttatttat ttgcaaaata gtcttccttg
     9901 gtcaagacat aatgaactct tgtcacgatt cggccttttt catgctggtc catacaagca
     9961 tatggttctg catgggaaaa acgcatgcct gatttctcca tgacctttcc tgacgcagga
    10021 ttgtccttat cgtgaagggc agtcaactta ttcatcccta tcttctcaaa agctagctca
    10081 atcacagcac gattggcttc tgtcgttaat ccttgattcc aatacttttt attgataatg
    10141 tagccaatag ctgccttctt aagaacagaa tcaatcttgt gcaagtcaat ggttccaata
    10201 aactgaccat tgctttttag ttctattccc caacgtccca agggattagc caaatagaac
    10261 tgagcaatgt tattcttggt ttcttccaag ctttgattgg ttggaaaagt gtaacgtgta
    10321 ttacccttgt ccgaggcata gtcaaacatt gcttccgcat catccaaagt tacaggtcta
    10381 agcaataaac gctccgtttc tatagacgga tactgggcaa gttttacaaa aattgattcc
    10441 atacttttcc aaccaatcaa agcttgattt tcactagtct agcataaaaa atttggatga
    10501 caagtttttc ttgatttttg aagcggtttc atatacaatg aaaccgtcac atttttgatt
    10561 tatgaactgg aatgtgacaa ccaaaacaag accgtcaatt gaaaggtagg ttatctcaat
    10621 gttaatcgga atcccaaaag aaattaaaaa taacgaaaac tgtgtcgccc tcacacctgc
    10681 aggtgttcat agcttagtta gtcgtggtca tcgtgtcctt atcgaaacaa atgctggtct
    10741 cggttctggc tttactgatg ctgactatca aaagcaagga gctgagattg tcgctactgc
    10801 tggtgaagct tgggcagcag agttggttgt gaaagtaaaa gaacctttaa gttctgaata
    10861 cggttacttg cgcgacgatc ttcttctctt cacctacttg cacatggccg ctgctccaga
    10921 attagcagat gctatgttag cagcaaaaac aacaggaatt gcctatgaaa ctgttcgtga
    10981 caatcaagga caactaccgc tcctcgttcc tatgagtgag gttgcaggtc gtatggctgt
    11041 ttaaatcgga gctcacttcc ttactaagca agctggtggc tctggtgttc tacttggtgg
    11101 tgtaccaggt gttccaaaag gaaaagtaac tatcatcggt ggtggtgtcg tcggtacaca
    11161 tgctgcccgc atcgcccttg gtcttggtgc tcaagtgact attttagata ttagtgccaa
    11221 gcgtctctca gttctagaag aagtctttgg aagtcaaatt caaactctta tgtctaattc
    11281 attcaacatt gaagcaagtg tgagagatgc tgatgtggtg attggagcca ttctcatccc
    11341 tggtgcaaaa gcaccggaat tggtgacaga tgagatggtc aaacaaatgc gtccaggctc
    11401 tgtatcgttg acgttgctgt tgaccaaggt ggcgttatcg aaacagctga ccgtgtgaca
    11461 acgcacgatg aacccgtcta tgaaaaacac ggtgttctcc actatgccgt tgccaatatc
    11521 cctggtgcgg ttgcccgcac ttcaaccatc gccctaacca atgtcactct tccttatatc
    11581 gaagctttgg ctggcaaagg attcgcacaa gcaatctctg aagatgaagg cttgcgtcaa
    11641 ggtgtgacca cttatcaagg ttacttgact agcctaccag ttgctcaagg acttaatcgt
    11701 gactacactg atatcaatga tttagtataa attttgaaaa gaaaagggtg acttcgcaag
    11761 tcctacccac tgtgctttgt gtcatcagct attacatcgt tggtttattg tactgtatac
    11821 tttgcaactg gagactttcg ttccagactc aattgtgcat aagaaactct aaattgaata
    11881 aagtcaacag agtttcgccc aaccacttcg tcttgtaatg acaattatat aaaccaaagg
    11941 aagctgagct ctttagtccc agcttctttt tatatataaa attttacccg tgcaaagaca
    12001 gggccttagc agacttcttt tttacttcgt tcacccttgc tttttctttt taggtttggg
    12061 cgttggcagt tggttataca tagctaaaat caggtcttat agaaacatct tattatcaag
    12121 ttcttccact caaatcattt ctttggcacc tttgtatgga aactcaaaag aagattggtc
    12181 aatcttatct aagactgctt gcacgggttt aactaaaaag cgatcatcat aaatgccgcc
    12241 aataatcttg ccgcggaagt aaagaatata ctcccccatc atggaacggt aagtcacatc
    12301 atctaatcct gataattgtt ccaaaacaaa ttccaaatag ttcttacttg atgccatttc
    12361 taatcttcta ggctctgttc agcgataaca accgtataga gttcttgctt aacctcgcat
    12421 ccaattgatt taaaaccctg cttttcccaa aaatgctgag attgcggatt tcccttaaca
    12481 taagccaaac gtgcctttcg aaagttctta gcaaaataag ctagtgcttc tgtcacaata
    12541 tgactaccaa tccctttcct ctgataggct tgatcaacca taaacaaacc aataaaaaca
    12601 gtctcctcat caggataggc atagacaaaa tccataacag ccacaaggtc agatccattc
    12661 caaaatccaa caaaaaactt atcagcctta gctttacctt caggtagaca aagcatgtcc
    12721 tcttttacag ttgcaaaatt tggctctggt ggacaatgct gaaaatacag aggattactt
    12781 tcatataaag ataaaatact tggaatatcc ttttcagtta gtatcctaca actgtaatac
    12841 ttagatagtt ggtcaatcat cttttcaaat tcctactttc ttgtgccctg tgattatgac
    12901 acaggaagat gcactgatcg tcatcagcca cataaaagtt ctttccatcg tgcctaatcg
    12961 ttgtctcaga cctttggata aaacctttag cctatacaac tggattttcc tctctcaaaa
    13021 gtatattctt ttgcaggcga acttcctcaa aatcagtcgt gtgcaacttc agtagaatat
    13081 tcataggctc ggataatctg agcgacaact ggatggcgaa ccacatcctt ggctgaaaaa
    13141 tgaacaaagt caatctgatg gatgttcttg agtttctctt gagcatcaat caaaccggac
    13201 ttgacattac gtggcaggtc aatctgactg atatctccat tgacaatcat cttagaatga
    13261 aagcctaaac gcgtcaagaa catcttcatc tgcatgatgg tcgtgttttg cgcctcatcg
    13321 agaatgacaa aggcatcatc caaggtccgg ccacgcatat aggcaagggg cgcaatttcg
    13381 ataatttcac gctccatgag acgagtcgtt tggtctttcc caagaatttg atacaaggca
    13441 tcgtaaacag gacgaaggta aggatccacc ttctccttaa gatcacccgg aagaaatcca
    13501 agactctctc ccgcttccac cgctggacga gttaggataa ttcgcttgac ttgcccacgt
    13561 ttaagggcag tcactgccaa ggtcactgca aggaaggtct tccctgtacc tgctggccca
    13621 attccaaagg tcacatcatg ctgtttgaca ctgtccacat aaagcttttg ccctagggtt
    13681 ttgacacgga taggtttccc agtattatct ttgataattt cttcttcgta aagggcgaca
    13741 aacttgtcaa tttcatcatt tttgaccatg ctaatcgcag tgactacatc tggcgtacca
    13801 acggtcatcc cacgatttac caagaccatc aaagcctgaa taacttgacg ggcttcctca
    13861 caggcagact cttctcccaa aacctggaca atctccgtac gagcatgaat cacaacatca
    13921 agctcttctt ccatcaaacg aagatggcgt tcattggaac caaaaagatg aaacaggtca
    13981 tctggatgac tcagttgaat gtctattgaa tgttccttca aataaagaac ctctctaatc
    14041 cttttatttc tttttattat agcagaggga tggagaaatt actagcccaa acccaatgaa
    14101 taaatgctag tgttctagat attcctgcca aatgctgtca aaatctccta ggttaaaaga
    14161 gaaactggca tgctcctcca aaaagcgact gacctcatca aaatcatctg tatgttttgg
    14221 aaaagctgcc tcttcaaaag cgaggtctgc caaaattgct ttgggactgt tacttttagg
    14281 attgcgctcg gtcatgagcc aagtgtaaaa tgattttctc ataagcctcc ctaaatcaac
    14341 tctgtcccaa actggtcctg cttgatttta ccagccttca taagaccacc taaagctttc
    14401 ttgaactgac ctttagaaat gccaaaggtt gccttgatgt cgtctggaga tgacttatca
    14461 tttaaggtca tgaaaccgcc attactttcc aaataagtca aaatcatctg agcatcgttt
    14521 tccaacattt caaaggagcg tggtttgagg gagaggttca gagtgcggtc cacttcacgg
    14581 aaaccaataa cgcgcgcatc taatacttgc cccaaacgtg gctctgcgta acgctcgcta
    14641 ggatgaataa aaccaagcat attattttct ggtaggtaaa caaaagttcc tgacagcttg
    14701 agacggtaaa caatggctgg ccagttttgg ttctgcatgt tgttgtaggc aggacgagca
    14761 agacgttgga agtcttcttg ataagccaag aggccccaga tacggtcttt cttatccact
    14821 tcaagacgga tgtagagttg gtcgcccttc ttaggccaga gttccttgag ctcagggaga
    14881 atatcgagtg acacaacgat ttccttgtca ggaaggcctg tatccacaaa gacacccaaa
    14941 tccttacgaa cctctgtgac acgtccccaa ccaaattggt cctgagtggc agtcacttct
    15001 aaggttgtca ggcggagttt ttgcttcata tccgtgtatg caaaaccttt gaccgtatcc
    15061 cctactgtat gttgaccttc ttccttagca agagcatagg tttgaccatc cttttgcaca
    15121 aagtaaaaac ggtcattttc atcgatgatc agtccaacga taaaacttgc aagatttgta
    15181 ttcatatttc cttctttcga ataaaactca gccagcaatg ccaactgagt ttttctgttt
    15241 atttttagac ttccaaaagt tctttctctt tgttagcagt catgtcgtcg atgtgtttaa
    15301 cagcatcgtc tgttactttt tgaatatctt tttcaagagt cttcaattcg tcttcagtga
    15361 tttcttttgc tttttcttgt ttcttagctt cgtccatagc atcgcgacgg atattgcgga
    15421 cagccacttt agcattttcg ccgaccttct tcacttcttt agcaaggtca cgacgagttt
    15481 cttctgtaag agctgggata accaagcgaa tcacagaacc gtcattagcc ggtgtgatac
    15541 caagatcaga agcgttcaag gcacgttcga tgtctttcaa tgaagacttg tcaaatggtg
    15601 ttaccaacaa aacacgcgct tctggaatcg taattgaagc gatttggtta agaggagttt
    15661 cgactccata gtattctaca tgtacacggt caagcaagct tgcattggca cgaccagcac
    15721 ggataccacc aaattcacga gcaagtgatt ggtgagattg ggtcattctc tctttagctt
    15781 tttcaataat tacgttagcc atattctttc ttattccttt tcttcgatat tatttgaaac
    15841 tgttgttccg atattttcac caaatacgac acgtttgatg ttgcctgatt ggttcatgtt
    15901 gaagacaacc aagtcaatgt cgttgtccat tgagagggtt gaggctgttg agtccatgat
    15961 acgaagacct ttgttgataa catcaaggtg ggtcaattct tcaaacttaa cggctgtctt
    16021 gtccttctta ggatcggcat tgtacacacc atcgacgcca tttttagcca tgaggatggc
    16081 atctgcttcg atttcagctg cacgaagggc cgctgttgta tctgtcgaga agtatggtga
    16141 accaattcca gcaccaaaga taacgatacg gcctttttca aggtgacgaa gggcacgtcc
    16201 acggacataa ggctctgcca cttgttgcat agcaatagct gtttgtacac gcgtatcaac
    16261 cccaacttgt tgcaatgaat ctgccatcac aagagcattc ataacagtcc caagcattcc
    16321 agtgtaatct gcctgaacac ggtccatacc tgcttctgct gcaggttctc cacgccagag
    16381 atttcctcca ccgataacaa gggcaatttc gatacctaag ctatgaactt cttgaatctc
    16441 ttttgcgatt gtttgaactg tttggatatc aatccctacg ccacgttcac cggcaagggc
    16501 ttcacctgat aacttgatta aaatacgttt atacttggga ttcgccattt tcactctcct
    16561 tctttcatcc tacctatttt atcacaattt ctaagatttt tatagtatca tgaacaattc
    16621 tttcaaaaaa attagacagt caaaaattcc tctaagtcgg caagggcacg ctctgcaatt
    16681 ttttcataac gagccttctt atcacggata cgctcgcctt ccaactcctt gatgatccca
    16741 aaattgacat tcattggttg gaaatgtttg ctgtcggcat gggtaatgta atgagctaag
    16801 cttccaatcg ctgtcgtctc ggggaaaata acctcgcttt cttccttgaa gagacgagct
    16861 gcgttaattc ccgcaactaa gcccgaagcc gccgactcaa catagccttc cacccccgtc
    16921 atctgaccag caaagaagag atttggttgt ttcttggaac ggtaagtctg ctcaagaaga
    16981 tttggtgaat ccatgtaaga attgcgatgc atgacaccat aacggacaaa ctccgcattt
    17041 tcaagacccg gaatcatttg gaagacacgc ttttgttctc cccatttgag gtgggtctgg
    17101 aaaccaacaa tattgtatag gctaccagct gcattatcct gacgaagttg cacaaccgca
    17161 taaggtgttt taaattctcc atcacgaggt cctgtgtagt cgtccggata ctcaagaccg
    17221 actggcttca tagggccata aagcatagtt ttaatgccac gtttggccat aacttcgatt
    17281 ggcatacatc cttcaaagta cttttctttt tcaaaagaat tgagcggtgc ttcttccgca
    17341 ttgaccaagg cttcatggaa atccataaac tcttgcttgg tcattggagc attgagatag
    17401 gctgcttctc ccttatcata acgagatttg agatagacct tactcatatc gatagtgttg
    17461 acatcgataa taggcgctgc tgcatcgtag aaatagaagc catcgccatt attaagggca
    17521 tgaatctttt cagccagggc atcgctagtc aagggaccag tcgccacaac tgtaataaca
    17581 tctgttggca attctgtaat ttcatcacgc accacttcaa tcaaggggtg gttgaccact
    17641 ttttcggtca ccatttgaga gaaaccatca cgatccaccg caagggctcc accggcagga
    17701 acacgtgtag cctcagcaga ttccaagata acagaaccca agcgacgcat ttcttccttg
    17761 agaagaccaa ctgcatttgt caaagcatcc ccacgcaaag aattggaaca aaccaactca
    17821 gcaaaattgt ctgttttatg ctggggtgta gacttgacac cacgcatttc atatagttta
    17881 actggaatac cacgctctgc gatttggtaa gctgcttcag aacctgccaa accagcaccg
    17941 ataacattga tataagattg agacacgaca ctaatacctc tttgggagtg tgaagttaag
    18001 attcacattg aaaaagccaa tcagacttac aagctttcga gtttcttggc tcaggctgaa
    18061 aaagtccaca ggactttttc actcccacaa atctttctat tttttcttct actagtataa
    18121 caaaaaaagg gaagaaggca aacttccctg tttagtcatt ttcttgatgt aaagagatag
    18181 tgagtattcc agttaagaat caattatatg ctactctata aaatcttttc cacataacgg
    18241 atcgataggg actgttattc tatctcttgc tataaccgta ttatctaaaa cagcataaca
    18301 ttcaacatag tgatctcctt taaactgtga atcttcgtga tattttattt tacctgaaaa
    18361 aatagcacca cgctcacaat ttcttctaat agcctcagct tcaatatttc ttactttcca
    18421 atagacttcc tgcgaaa
//
LOCUS       DBERCA010000038        18117 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.038, whole genome shotgun sequence.
ACCESSION   DBERCA010000038 DBERCA010000000
VERSION     DBERCA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.038"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            166..1647
                     /gene="mgaSpn"
                     /locus_tag="ACL719_001428"
                     /gene_synonym="mgrA"
     CDS             166..1647
                     /gene="mgaSpn"
                     /locus_tag="ACL719_001428"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGS0699379.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2008..3009
                     /locus_tag="ACL719_001429"
     CDS             2008..3009
                     /locus_tag="ACL719_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0699380.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3159..4076
                     /locus_tag="ACL719_001430"
     CDS             3159..4076
                     /locus_tag="ACL719_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699381.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYDYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4087..4977
                     /locus_tag="ACL719_001431"
     CDS             4087..4977
                     /locus_tag="ACL719_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0699382.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5006..6622
                     /locus_tag="ACL719_001432"
     CDS             5006..6622
                     /locus_tag="ACL719_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0699383.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKATDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTIPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSNYLAIKQKYYDQYQANKN"
     gene            6632..7951
                     /locus_tag="ACL719_001433"
     CDS             6632..7951
                     /locus_tag="ACL719_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS0699384.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8159..8231)
                     /locus_tag="ACL719_001434"
     tRNA            complement(8159..8231)
                     /locus_tag="ACL719_001434"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8196..8198),aa:Lys,seq:ctt)
     gene            complement(8496..9767)
                     /locus_tag="ACL719_001435"
     CDS             complement(8496..9767)
                     /locus_tag="ACL719_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGS0699385.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            10059..10280
                     /locus_tag="ACL719_001436"
     CDS             10059..10280
                     /locus_tag="ACL719_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699386.1"
                     /translation="MEMMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEI
                     LDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10445..10624
                     /locus_tag="ACL719_001437"
     CDS             10445..10624
                     /locus_tag="ACL719_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699387.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10761..10940
                     /locus_tag="ACL719_001438"
     CDS             10761..10940
                     /locus_tag="ACL719_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGS0699388.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            10977..11429
                     /locus_tag="ACL719_001439"
     CDS             10977..11429
                     /locus_tag="ACL719_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGS0699389.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11720..12190
                     /locus_tag="ACL719_001440"
     CDS             11720..12190
                     /locus_tag="ACL719_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGS0699390.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEKGEFYPYPKY"
     gene            12209..13297
                     /locus_tag="ACL719_001441"
     CDS             12209..13297
                     /locus_tag="ACL719_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGS0699391.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENWLAELEKEMLEGTEDGTRNF"
     gene            13278..13706
                     /locus_tag="ACL719_001442"
     CDS             13278..13706
                     /locus_tag="ACL719_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0699392.1"
                     /translation="MELEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13842..14792
                     /gene="prmA"
                     /locus_tag="ACL719_001443"
     CDS             13842..14792
                     /gene="prmA"
                     /locus_tag="ACL719_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGS0699393.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            14794..15537
                     /locus_tag="ACL719_001444"
     CDS             14794..15537
                     /locus_tag="ACL719_001444"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGS0699394.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15548..17344
                     /gene="pepF"
                     /locus_tag="ACL719_001445"
     CDS             15548..17344
                     /gene="pepF"
                     /locus_tag="ACL719_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS0699395.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFENFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(17404..18081)
                     /locus_tag="ACL719_001446"
     CDS             complement(17404..18081)
                     /locus_tag="ACL719_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGS0699396.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         5621 a         3109 c         3937 g         5450 t
ORIGIN      
        1 ctcaatagac ggtaaaataa gaaaattgtt tatatattgt ctccgtagtg ttattatacg
       61 aaataaaaga ttttaggaaa agtcgttata tcatgctata cctattcttt gtggtataat
      121 tgcaagaggt ttaatccgat aatttataaa gagagaaaga tattcatgag agatttatta
      181 tctaaaaaaa gtcataggca attagaatta ttagaattat tatttgaaca taaacgttgg
      241 tttcatcgtt ctgaactagc agagttatta aattgtacag aacgtgcagt caaagatgat
      301 ctatcccatg ttaaatctgc ttttcctgac ttgatttttc attcttctac taatggtata
      361 cgcattatta ataccgatga tagtgatatt gaaatggttt accatcattt ctttaaacat
      421 tcaactcatt tttcgatttt agaattcatc ttctttaatg aaggttgtca agctgagagt
      481 atttgtaaag aattttatat cagttcatct tcgctctatc gtattattag ccaaatcaat
      541 aaagtgatta aaaggcaatt tcaatttgaa gtcagtttga cccctgttca aatcattgga
      601 aatgagagag acattcgtta cttttttgca caatattttt cagaaaaata ttatttccta
      661 gaatggccat ttgaaaattt ttcatcagag ccactatctc aattgttaga attggtttat
      721 aaggaaacaa gctttccaat gaatttgtca acgcatagaa tgctaaagtt gctcctagtt
      781 acgaacctat atagaataaa gtttggtcat tttatggaag tagataaaga ttcttttaac
      841 gaccaaagtt tggatttttt aatgcaggca gaaggaatag aaggtgttgc tcagagtttt
      901 gaatcagaat acaatatctc tttagatgaa gaagttgttt gccaattatt tgtgtcttac
      961 tttcaaaaaa tgtttttcat agatgaaagt ctctttatga aatgcgtaaa aaaggatagt
     1021 tatgttgaaa aatcttacca tctattgagt gattttattg atcagatttc agtcaagtat
     1081 cagattgaaa tggagaataa ggataatctg atttggcatc tgcataatac cgcacatctg
     1141 tatcgtcaag agttgtttac tgagtttatt ttatttgatc aaaaagggaa tacaatcagg
     1201 aactttcaaa atatttttcc aaaatttgtt tcagatataa aaaaagagct ttctcactat
     1261 ttagagactc ttgaggtttg ttctagttca atgatggtaa atcatctatc ttataccttc
     1321 atcactcata ccaaacattt ggtgattaat ttgctacaaa atcagcctaa actaaaggtt
     1381 ttggttatga gtaattttga tcagtatcat gcaaaattcg ttgcagagac actttcttat
     1441 tactgtagca ataattttga actggaagtt tggactgaat tagaattatc aaaggaatct
     1501 ttagaagatt caccttatga tatcattatt tctaatttta ttattcctcc tattgaaaat
     1561 aagagactca tctattcaaa taatataaac acggtctcac tcatatattt gttaaatgcc
     1621 atgatgttta ttcgattaga tgagtaacca aaaataacaa aaaacgctat ttcgcgtttg
     1681 aactgtacct caaaagttag acagaaaaaa tctaattttt ggggtgtttt tgttaagaaa
     1741 agaccttcaa tcatttaagc aaaatgtaca tctagattta tagccctcta aaactcttgc
     1801 tttttttgta gaagatacga aaaaagccat aaaaccaagg cttttcctgt tttatgtaga
     1861 tgtttacaca tgaaaattga agtattttca aaagctaatt ggagtgattt tattcatgat
     1921 gggataaatg atctggccaa aaatgattga gaaatagttg cttaaaaagc atttgcatga
     1981 tactatatat tgaaaggagg tgataatatg gtagaacaaa gaaaatcaat taccatgaaa
     2041 gatgttgctt tagaagcagg agttagtgtt ggaactgttt cacgtgtaat taataaagaa
     2101 aaaggcatta aagaagtaac tttgaaaaaa gtggaacaag cgattaaaac tttgaattac
     2161 attccagatt actacgctag aggaatgaaa aaaaatcgaa cagaaacgat tgcaatcatt
     2221 gtaccaagta tctggcatcc cttcttttca gaatttgcta tgcatgtgga aaatgaagtc
     2281 tataagagaa ataacaaatt actcttatgt tctatcaatg gtacaaatag agagcaagac
     2341 tatctggaga tgttgcgtca taataaagtt gatggagtgg ttgccattac ctatagtcca
     2401 attgaacatt acttgacgtc aggaattccc tttgttagta ttgaccgcac atactcagat
     2461 attgccattc cttgtgtttc atcagataat gatgcaggtg gaagagaagc agctaaacaa
     2521 ttaattagta aaggatgtca gcatttagcc tttgtgggcg gacataatac aaccattaat
     2581 gaaaccaaac gtcgtagaat ttcatttgaa aagtatgtcc aagagcataa aatacctctt
     2641 agtatttttg atttggatga gacagttact gactattatg gaaagctaga gagtttcttg
     2701 gaagaaaatt cttctataga tggaattttt acaatcaacg attttacagc tttggatgtg
     2761 attgaaatac tagaaaaaaa tggcaaacgt attcctcatg atgtccaaat tattggatac
     2821 gatggaatta aaatggctgg agatagagat tatctacttt caactattga acaaccattg
     2881 gaagaaatgg caaaagaagc ggttcgtatt ttgtttgata taattgatgg aaagactgtt
     2941 aatttgcaga caatcctacc agtaaaattt gttgaaggaa aaacaacaaa aaatgaaaat
     3001 aagtcttgac agaaagcgct atcaatgata gaatgaattc agataaaaag atttattttt
     3061 aaaacaaaaa tgaaacgttt caaaaaaaga aataaagaga cagcgccaag cgctatcttt
     3121 tctagaaaaa aatgaaacgt ttcaaaaagg aggttgctat gaatagcaaa gcgaagcaag
     3181 tttctctttg ggaaagaatc aagaaacaaa aactcttgtt attgatgact gtccctggtt
     3241 tagttttaac ctttatcttt aaatacatcc ctatgtatgg ggttttaatt gcatttaaag
     3301 attacaatcc tttaaaagga attttaggga gtgattggat tggtttttct gagtttacaa
     3361 aattcatatc ctctcccaac tttggtatct tgttagccaa cacattaaaa ttaagtatct
     3421 atggtttatt gcttggcttt ttaccaccaa tcattcttgc tattatgctc aatcaactct
     3481 tgagtgaaaa agtcaaaaaa cgaattcagc tcattttata cgcaccaaac tttatctcag
     3541 tcgttgttat tgtcggtatg attttcctct tcttttcagt gggaggacca atcaacaatt
     3601 ttctttctat gtttggaatg aaggctgact tcttgacaaa tccagacttc tttagacctt
     3661 tatacatctt tagtggtatc tggcaaggaa tgggctgggc ttcaacgctc tacacggcaa
     3721 cattggtaaa tgtagatcca gccttagtag aagcagcccg actggatgga gccaatatct
     3781 tccaacgaat ctggcacatt gatattccag ctcttaagcc tattatggtt atccaatttg
     3841 ttttagctgc aggtggaatt atgaatgtcg gatatgaaaa agcattcttg atgcagacat
     3901 cgttaaattt gccaacttct gaaattatct cgacatatga ctataaagtt ggtcttgtat
     3961 caggagacta ttcttactca acagcggttg gtttgtttaa tgcagtgatt aacgtagtat
     4021 tgcttgttgc agttaaccaa atcgttaaac gcatgaataa tggtgaagga atttaaggag
     4081 gaaagtatga aaaattcgat tatggataca aaatttgata gacgtatctt actcttaaat
     4141 aaaatcatta ttgtctttat cgttttgatg actttgcttc ctttacttta tatcgtcgta
     4201 gcatccttta tggatcctaa ggttctggtt agtagaggga ttagctttaa tccagccgat
     4261 tggactgtag aaggttacca gcgtgtattc agtgaccaat ctattctaag aggttttatc
     4321 aattctctac tatactcttt tggatttgca gctttaacag tcttgctatc tgtgtttaca
     4381 gcttatcctc tttccaaaaa agatttggta gggcgccgtt ggattaacta cttcttgatt
     4441 gtgactatgt tctttggtgg tggtttagtc ccaacttact tgctcgtaaa agaattggga
     4501 atgctcaata ctccatgggc tatcattgtt ccaggtgctg tcaacgtttg gaatattatt
     4561 cttgctaggg cctatttcca aggattgcct gaagaattag ttgaagctgc tgtcattgat
     4621 ggtgcaaatg atttacagat tttcttcaaa atcatgcttc ctcttgcaaa accaattatg
     4681 tttgttctct tcctttatgc ttttgtagga cagtggaact catactttga tgcaatgatt
     4741 tatatcaagg atccaaactt ggaaccattg caacttgtac ttcgtaaaat tctcattcag
     4801 agccaaccag gtcaagacat gattggagca caagcggcta tgaatgaaat gaaacgttta
     4861 gctgaattga ttaaatacgc aactattgtc atttccagct tgccattgat tgttatgtat
     4921 ccattcttcc aaaaatactt tgataaagga attatggctg gttcacttaa aggataaaaa
     4981 aagaaaaaat aaaaggagtt ttctcatgaa attcaaaaca ttctcaaaat cagcagtttt
     5041 gttgacagct agtttagcag tacttgcagc ctgtggctca aaaaatacag cttcaagtcc
     5101 agattataag ttggaaggtg taacattccc gcttcaagaa aagaaaacat tgaagtttat
     5161 gacagccagt tcaccgttat ctcctaaaga cccaaatgaa aagttaattt tgcaacgttt
     5221 ggagaaggaa actggcgttc atattgactg gaccaactac caatccgact ttgcagaaaa
     5281 acgtaacttg gatatttcta gtggtgattt accagatgct atccacaacg acggagcttc
     5341 agatgtggac ttgatgaact gggctaaaaa aggtgttatt attccagttg aagatttgat
     5401 tgataaatac atgccaaatc ttaagaaaat tttggatgag aaaccagagt acaaggcctt
     5461 gatgacagca cctgatgggc acatttactc attcccatgg attgaagagc ttggagatgg
     5521 taaagagtct attcacagcg tcaacgatat ggcttggatt aacaaagatt ggcttaagaa
     5581 acttggtctt gaaatgccaa aagctactga tgatttgatt aaagtcctag aagctttcaa
     5641 aaacggggat ccaaatggaa atggagaggc tgatgaaatt ccattttcat ttattagtgg
     5701 taacggaaac gaagatttta aattcctatt tgctgcattt ggtatagggg ataacgatga
     5761 tcatttagta gtaggaaatg atggcaaagt tgacttcaca gcagataacg ataactataa
     5821 agaaggtgtc aaatttatcc gtcaattgca agaaaaaggc ttgattgata aagaagcttt
     5881 cgaacatgat tggaatagtt acattgctaa aggtcatgat cagaaatttg gtgtttactt
     5941 tacatgggat aagaataatg ttactggaag taacgaaagt tatgatgttt taccagtact
     6001 tgctggacca agtggtcaaa aacacgtagc tcgtacaaac ggtatgggat ttgcacgtga
     6061 caagatggtt attaccagtg taaacaaaaa cctagaattg acagctaaat ggattgatgc
     6121 acaatacgct ccactccaat ctgtgcaaaa taactgggga acttacggag atgacaaaca
     6181 acaaaacatc tttgaattgg atcaagcgtc aaatagtcta aaacacttac cactaaacgg
     6241 aactgcacca gcagaacttc gtcaaaagac tgaagtagga ggaccactag ctatcctaga
     6301 ttcatactat ggtaaagtaa caaccatacc tgatgatgcc aaatggcgtt tggatcttat
     6361 caaagaatat tatgttcctt acatgagcaa tgacaataac tatccaagag tctttatgac
     6421 acaggaagat ttggacaaaa ttgcccatat cgaagcagat atgaatgact atatctaccg
     6481 taaacgtgct gaatggattg taaatggcaa tattgatact gagtgggatg attacaagaa
     6541 agaacttgaa aaatacggac tttctaatta cctcgctatt aaacaaaaat actacgacca
     6601 ataccaagca aacaaaaact agaggttgat tatgggagat aagaaataca cagtagaaaa
     6661 agccaatcgt tttatagcag aaaataaaca tctcgttaat actcaatata agcctgaaga
     6721 acatttttca gctgagattg gttggatcaa tgatccaaat ggatttgtct attttcgtgg
     6781 agaataccat ctcttttatc aattctatcc atatgatagt gtttggggac ctatgcactg
     6841 gggacatgct aaaagtaagg acttggtgac ttgggagcac ttgccagtgg cacttgctcc
     6901 tgatcaagat tatgaccgaa atggttgttt ctcaggctct gccattgtca aggatgatcg
     6961 cctctggctc atgtacactg ggcatatcga agaaaaaacc ggtgtccgcc aagtgcaaaa
     7021 tatggcattt tcagatgacg ggattcactt tgaaaagatt tcccaaaatc cagttgcaac
     7081 tggatcagac ttgccagatg agttgattgc tgctgatttc cgtgatccaa aactctttga
     7141 aaaagatgga cgctattact ccgtagtagc tgccaaacac aaggataatg tgggctgtat
     7201 cgttctacta gggtccgata acctagtaga atggcagttc gaatccatct ttttaaaagg
     7261 gggagaacac caaggcttta tgtgggaatg cccagattac ttcgagttag atgggaaaga
     7321 ttgccttatt atgtcaccca tgcgttatca gcgtgaggga gactcatatc ataacatcaa
     7381 ctcatcgctt ttgttcacgg gtaaggtaga ttggagagaa aaacgtttta tcccagaatc
     7441 agttcaagaa attgatcatg gccaagactt ctatgcgcct caaacattgt tggacgatca
     7501 aaatcgtcgt atcctgattg cttggatgca gacatggggg cgtacccttc caacccatga
     7561 ccaagaacac aaatgggcat gtgctatgac tttacctaga attctaagat tggaagatgg
     7621 caaactaaga caattccctg ttaaaaaagg ccaatatcaa atccaaatag ataaagattg
     7681 tcattaccac ttaggaaatg atacagatta tcttgaattt ggttatgaca gtaatgcgca
     7741 gcaagtttac attgatcgta gccatcttat tcaaaaaatt ctaggtgaag aagaacagga
     7801 cactagtcga cggtatgtag atattgaagc taaagaattg gaagttgttc tagataaaaa
     7861 ttccatcgag atttttgtca atcaaggtga agcaagcttg actgcaactt attacttaac
     7921 ggtgccagct gagctatcac gaattgatta aaaattaagt tatttctcct aaagaaaaag
     7981 ttctctttct aaaatagtgg aaagaggact ttttgtgttt tgggtatata agcttagttt
     8041 atggtatttg taaaattggt gttggattat gatttaagct agttttctaa agaatttgaa
     8101 aaaaatttta tttaagcaaa aaaaccttgg ttccaaggct tttcctgttg tatgtagatg
     8161 ccccctacag ggatcgaacc tgtgacccac ggattaagag tccgctgctc tgccagctga
     8221 gctaaggagg caaagaaaaa agctgtattg gtgccgaaac ttcacggttt gtattgaacc
     8281 cgcgcaatta agcaggtggg caactcgctc taactgaagc tgtttccgtg tgagacggcc
     8341 tacatgctgt tagaagactt ttgtttccct aataatacaa aaaatagtcg gtcaacactt
     8401 aagtgtgaag tcgtacacca cagcgtttct atacttatat gataccactt tttcaaaaaa
     8461 aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga tttttcgcaa acgatcgata
     8521 gcttcctttc tttgagccaa agcccgttca tatttaccag tatcttctgc ttggaaatag
     8581 tgatgattac gaattttttc tggcagatag tcttgcttga tccaatttcc aggatagttg
     8641 tgtggataga gatagtcttg ggcattcccc agttccttgc ttccactgta gtgcccatca
     8701 cgcaggtgtc gcggaatagg caagtgccct gatgttttga ggtcagcaag tgccttatcc
     8761 atagctacat aggctgagtt ggattttgga gaaagggcca aatcaatcac gacattggca
     8821 atgagaatgc gggcttctgg gaacccaatc ttctgggcag catccagagc agtcacggta
     8881 tgaatctggg cctcaggatt ggctaagccg atatcttcat aggcgataac agtcaagcga
     8941 cgagcgagac taggcagatc cccagcctca atcaagcggg cagtatagtg gagactggca
     9001 tccacatctg agccacgaat agacttttgc agggcagata gaacatcata gtgaccgtct
     9061 ccatccttgt ccatagtgat gtagcttctc tgaagactat tttccatgat gtctaaggtg
     9121 atgtggcgaa tgccctcgtc attctcaggg gtagagagaa cagctaagtc cagtgagtta
     9181 aaggcagagc gaaggtctcc gtttgtagag gttgcgatga aatccagcgc atcctcatct
     9241 agttctattg gaaaatcaaa accacgttca gggttactta gagctatctg cagggcctct
     9301 ttgacgtctt ggttagacag aggttccaac tcgaaaattt gaactcggct acgaatggca
     9361 ggagtgacag agaagaaagg attttcagtc gttgctccaa tcatgatgac cagtccactt
     9421 tccaagagag gcaagaggaa gtcttgcttg gtcttatcta gtcgatgaat ttcgtctagt
     9481 aataggacga gaccaccaga aaatttagct tcttccgaga tttcttgcag tcgcttttta
     9541 ctatcaactg tcgcattgaa agttcgaaag gcatacttgg tcgttccagc gatggcagag
     9601 gcaatactgg ttttgccgat tccaggaggg ccatatagaa tcatggagga caggcggttg
     9661 gcttccacca tgcggcggat gatttttcca ggtccgacca gatgctcctg accgatgacc
     9721 tggtcgatgg ttttagggcg catgcgaagc gcgagattgt ctggcatagc agtcctttct
     9781 aacgtggatt tgagtagaat gactcatcta ctatatctta ctatgtccat tctagcctat
     9841 tagttgtgat atttcaatgg ttttgtaata atcagggagt gagatagaat cttttttact
     9901 atttaattta atgaggagag gtatcagcat gagagatttg attttcctta aagctcaaaa
     9961 taggatagtt gagcctaaag aagtggaagt gctgacttga gaaataatat tgctgaatgt
    10021 cattgtatga actataatga aaagagaggt gataagcaat ggaaatgatg gaactaccaa
    10081 gtcaggaaat tttgattttt acaaaacaaa ttcgtcattg gattcttagt gatcaagtta
    10141 tttcagggaa aagaaaactt ttcttccggg aagatactcc aaaagagatt ttagatttgt
    10201 acgaaaacat taaatctaaa cttgattttg cttaccaaga agttcattct aataatgggt
    10261 tgaaaaaata tgaaaaatag aataattgat gtttttgaag tagtaaatcg cctccttgta
    10321 ataactgttg aaaatcctga tttcgaagac ttgagagtta atcatactca atgaaaatca
    10381 aagagcaaac taggaaacta gccgcaggct gtacttgagt acggtaaggc gacgttgacg
    10441 tggtttgaat ttgattttcg aagagtatca gtttgtaaaa attggagata aaaaatatag
    10501 agtacgcagt gttcctatga ttcattcaac tccgcctcaa tctgtcttag atcgagatac
    10561 ttttacaatt gactatacag atgatgaatt gctcgataaa gaagcagttt ttactactca
    10621 ttagaaaaat aggtatgaat aaattggctt cccttagaac cacatctagg ggcttttatg
    10681 tataatagaa taataagtat cactccttta caatacgtgt aacacgtgtt ataatggtgt
    10741 tgtcaggagg taaaagcgct atgcctatga cacaaaaaga gatggttaaa ctccttacgg
    10801 cccatggttg gataaagact agaggcggta aaggctccca tattaaaatg gaaaagcaag
    10861 gggaaagacc tatcacaatc cttcatggtg agctgaataa gtacactgaa agagggataa
    10921 gaaagcaagc tgggttgtaa aattcagccc ctgctttttg atatggaggt agtgaaatgt
    10981 tagttacgta tccagcttta ttttattatg acgacacaga cggaacagaa gcgacttatt
    11041 ttgtccattt cccagatttt gaatactcag ctacacaagg agaggggatt tctgaggctt
    11101 tggctatggg gtcggagtgg ctagggataa ctgttgcaga tttgatagag agtgatggag
    11161 agttacctca gccatcagat atcaatagct tgtctttaat tgataatgat ccttttaaag
    11221 atgatgaaga tttcgtgtca acctatgacc ttgataaatc ttttatttct atggtatctg
    11281 ttgatgtatc agaataccta ggaagtcagg aacccattaa aaagactttg accataccaa
    11341 aatgggcaga taagttggga cgagaaatgg ggcttaactt ttctcagact ttgacagacg
    11401 ctattgcaga taagaaagtt caagcctaag attaagcgtg gtatcgtggt aatagtcaat
    11461 aaaaaagcac gtccacttgt gctagttact cgcctattga ttttatagat ttattagccc
    11521 tttcgagggc tttttatatt gatttttatg agaaatgaag aaatcagact tttaagaaaa
    11581 tcactgatac caagggttta aatgaggtaa tatggtataa ttaggacata aaataatttt
    11641 gtggtaagat ggtagtatct attttagcat atttccgagc aatcggggcg attaaagagt
    11701 cgcatagaaa gaggacaaaa tggcaacata cggattttta gatattttag aggaagagtt
    11761 ggacaagaac tttccctttg actttgagat tagctgggac aaacgcaacc atgcggtaga
    11821 agtgagtttt ctattggaag cgcaaaatgc tgcaggtgtg gagatggtgg atgaagacgg
    11881 agaggtttca tcagatgaca ttctttttga ggaagcggtc cttttctaca atcctgccaa
    11941 gtcaacagtc aatgaggaag actatttgac tgttattcct tacctgccta aaaaaggatt
    12001 ttcccgtgaa tttttagctt attttgcgct tttccttaaa gatactgctg aggttgggct
    12061 agatgtcctc atggactttt tggaagaccc agaagcagag gaatttgtca tggaatggaa
    12121 ccaagaagtc tttgaagaag gaaaaattgg cttggaaaag ggagaatttt atccttatcc
    12181 aaaatactag gagttggttg gaggtgttat gaagaaaatt gggacttatt tggtttatgt
    12241 gctagctgtt gtctttatta tgctagcttt tgcttgtgga acaatcgcat ttgcagagtt
    12301 ggggtattcc gcagttttag cctttacttt tggttatgcc ttcgctcttc taagcatgta
    12361 tttaatcttt attcttcatg agctgggtca tgctttttgt ggctacttga caggctatcg
    12421 gctggtggct tttggattag gacattttat tttgaccaaa aagtcaggca ggtttcatct
    12481 tagtagaaca gccattctga aaaatgttgg tgctcaatac attggtttaa aagaggatga
    12541 aagcgatcaa agaatcatcc tgatgctttc aggaggcttg atggttcatc tcggcttgat
    12601 attattggcg atattgtttg gatttttgac aagaagctgg tattttgcag ggacttggat
    12661 ttttcttaat ttatctttct tcctaaataa cattttgcca gtcgacatca ccgatggagc
    12721 aaaaatttgg gaattactac aacaccctga aaataccaaa tacgcctacc tggtattgag
    12781 acattctgcc cagaccttgc tagctcctca agaatatgat ttgaaagact ttgttcaagc
    12841 agttcctgag gatgcacgag ggagttttgc ggatggtgta ttaggtatgc aaggtgaagt
    12901 ttccattttg gaagggaagg aagaagtagc taaacagcag tttcaagcat tacttgagag
    12961 gacaactact ccgatgatgc agacagttgc ccaactatcc ctgcttcata tcgccttgtt
    13021 ggaaggagat tttgagcaag cggagcaata tgctagtatt cgacaggtca aatcgtttct
    13081 gtctctcaaa ctgtccaatc ttcaagtgat acaagcctgg tatcaattaa aggtgaagaa
    13141 agatgtagat cgaactcgta aggctctcaa gattgctaga cagaaaatgg atagtagtcg
    13201 aatgttgagg gatgagaaaa gatattatga aaactggtta gccgagctgg aaaaggaaat
    13261 gttagaagga actgaagatg gaactagaaa tttctgattt cacaggttgc aagattgctt
    13321 tgttttgtgg ggataagctt ttgactatct tacgcgatga taaggcaagt attccctggg
    13381 ccaatatgtg ggaactgcca ggtggtggac gtgaaggtga tgaaagtcct tttgagtgtg
    13441 tagcgcgtga agtttatgaa gaactgggca ttcatctgac tgaagattgt ctgctttgga
    13501 gtaaggttta cccaagtatg ctttttgcag acaaacagtc cgtttttcta gttggtcagt
    13561 tgacacagaa ccagtttgac agtatcgtat ttggagatga aggacagggc tatcagctga
    13621 tgaatgttga ggaatttctt agttccagtc aagttgtgcc tcagttgcaa gagagattaa
    13681 aagattattt aaaagtaagt gattagaaag gatggttcgg ttaaatttct aaactgaacc
    13741 cgccctaaac acggtgccaa aaagataaac ttctcttaga cacaagcgtc ttcagagaat
    13801 ttcctatttt ggttttgtgt tttacgggct tggtattaat aatggaaaca tggcaagagt
    13861 taaaagttac agtgaagcgt gagggagagg agttggtttc caatctcttg attgagctgg
    13921 gagcgcaagg agtcgcaatc gaagacagta tggactatgt gggaaatgtg gaccgctttg
    13981 gagagatttt cccagaggtg gagcagcaag aagaaatcgt agtgacagcc tactaccctg
    14041 acacggtgga tgtgacagtg gttgaggcgg atttgcaggc tcgtctagca gaattgacag
    14101 attttatgga tttgggagag ttaaaaatag gtacgactgc cttggctgag gaagactggg
    14161 cagacaactg gaagaaatac tatgaacctg ctcgcatcac tcatgatttg accatcgttc
    14221 cctcttggac agactatgag gcgactgcgg gagaaaaaat tatcaagctg gatcctggca
    14281 tggcttttgg tacgggaacc cacccaacta ccaagatgag cctttttgcc ttggaacagg
    14341 ttcttcgtgg tggcgaaacg gtgctagatg tgggaactgg ttcaggggtt ctctctattg
    14401 ccagctcgct tctgggggct aaagaaattt tcgcctatga cctggatgat gtggcagttc
    14461 gtgtggctca ggaaaatatt gagctcaacc ctggaatgga aaatatccat gtagcggcag
    14521 gcgatttgct taaaggcgtg gagattgaag cagatgtcat cgtggcaaac atcttggcgg
    14581 atatcctcat tcatctgaca gaggatgctt atcgcttggt taaggatgaa ggttacctga
    14641 tcatgagtgg cattatcaag gataagtggg acatggtgcg cgagttggct gaatcagctg
    14701 gatttttcct cgaaactcac atggttcaag gggaatggaa tgcctgtgtc tttaagaaaa
    14761 ccaaggatat ctctggtgtg attggaggct agcatgcagc agtattttgt aaaaggcagt
    14821 gctatctctc ctgtcactat cgagaacaag gaaaccagta agcatatgtt tcaggttatg
    14881 cgcttgaaag aagaggatga ggttacctta gtctttgatg atggcatcaa gcgcttggcg
    14941 cgcgtgctgg atatggaaaa tcgtcagttt gagttggtcc aagaattaaa tgacaatgtg
    15001 gaactaccag tccaagtgac catcgcatct ggatttccca agggagacaa gctggagttc
    15061 atcgctcaaa aagtaaccga actgggtgct agccaaatct gggcctttcc tgcagactgg
    15121 tcagttgcca agtgggatgg caagaaattg ggtaaaaaag ttgaaaaact agaaaaaatt
    15181 gcccttggag cagccgagca aagcaagcgt aatattgtac caagtattaa acttttcgag
    15241 aaaaaagcag attttctagc tcagctggac cagtttgact ctatcatagt agcctatgaa
    15301 gaatcagcta aagaaggaga agccgctgcg cttctgcaag cagtcactgg tcttgaaaca
    15361 ggagccaaac tgctctttat ctttggtcca gaaggcggtc tgtcacctgc agaaatcgaa
    15421 agttttgaag ctaagggagc cgttttggta ggcctaggtc ctcgtatttt gcgagcagaa
    15481 acagcaccgc tttacgcctt atcagccctt agtgttttag tagaattaga gaaataagag
    15541 gaagaaaatg gaacaaaaac accgttcaga atttccagag aaggaactct gggacttaac
    15601 agccctatac caagaccgtg aggatttctt gcgtgcaatc gagaaagctc gcgaagacat
    15661 caaccagttt agccgtgatt acaagggcaa tcttcacact tttgagaatt tcgagaaggc
    15721 ctttgcggaa ttggaacaga tctacattca gatgagccat attggcaact atgcttttat
    15781 gcctcagacg acggactata gcaatgacga atttgccaat attgcccaag ctgggatgga
    15841 atttgaaaca gatgccagcg tagccttgac cttctttgac gatgccttgg tggtagcaga
    15901 cgaggaagtc ttggaccgtt tgggtgaatt gcctcacttg acggcagcta ttcgtcaggc
    15961 caaaatcaaa aaagcccact atctaggggc tgatgtggag aaggccttga ccaatctcgg
    16021 tgaagttttc tatagtccgc aggacattta tactaagatg cgagctgggg attttgaaat
    16081 ggctgacttt gaagcccatg gcaagaccta caaaaacagc tttgtcacct atgagaattt
    16141 ctaccaaaat cacgaggatg ctgaggttcg tgagaaatcc ttccgttcct tctcagaggg
    16201 acttcgtaag caccaaaata cggctgcagc agcctatctg gctcaggtca agtctgaaaa
    16261 actcttggct gatatgaagg gatatgactc tgtctttgat tatcttctag ctgaacaaga
    16321 agtggaccgt gtcatgtttg atcgccagat tgacctcatc atgaaggact ttgcaccagt
    16381 cgctcagaga tacctcaagc atgttgccaa ggtaaatggt cttgaaaaga tgacctttgc
    16441 agactggaaa ttggacttgg acagcgccct gaatcctgaa gtgactattg acgatgccta
    16501 tgatttggtc atgaagtcgg tagaaccttt ggggcaagaa tattgtcagg aagttgctcg
    16561 ttaccaagaa gagcgctggg tggactttgc tgctaacagt ggcaaggatt ccggtggtta
    16621 tgcggcggac ccatatcgcg tacaccctta tgtcctcatg agctggacag gccgtttgag
    16681 cgatgtctat accttgattc atgaaatcgg gcattctggt caattcatct tttcagacaa
    16741 tcatcaaagt tacttcaatg cccatatgtc gacctactat gttgaagcac cgtcaacctt
    16801 caatgaattg ctactcagtg attacttaga gaaccaatct aatgacccac gtcaaaaacg
    16861 cttcgctctg gctcatcgct tgacagacac ctacttccat aactttatca cccacctctt
    16921 ggaagcagcc ttccagcgta aggtgtatac attgattgaa gaaggagaaa ccttcggagc
    16981 aagcaaactc aacagtatta tgaaggaagt tttgacagat ttttggggcg atgccattga
    17041 aatcgacgac gatgcagctt tgacttggat gcgccaagct cactactata tgggcttgta
    17101 tagttacact tactcagcag gactagttat ctcgactgct ggttaccttc atctaaaaca
    17161 ttctgaaact ggagctgaag actggctcaa tctccttaaa tcaggtggta gcaagacacc
    17221 acttgagtca gccatgatta tcggtgctga tatttcaaca gacaaaccac tccgtgatac
    17281 catccaattc ttgtctgaca cagttgacca gattatcgcc tacagtgctg agttggggga
    17341 gtaaggggat agagaggttg ttatagaatg ggtatggaca gttatttgct taaaattcca
    17401 tgtttaaatc ccccattcct tttcgaggaa ttctttgccc ttctctgtaa tggtaagtga
    17461 acgtggcgtt tgtgcaggga tgaccaattg tttttcactg aaaaattcta aaagagcatt
    17521 gccgagagct ccgccgacat gatttttacg ttcgctccaa tccaaacatt tagggcaaaa
    17581 tttccgtttt ttctttttca attcatccgt atcaatgtgt aattttcttt ggaaataaga
    17641 cttcccagct tcagtgagtt ggaagtcttt ttctgattcg ctgaggagat tttcttgcaa
    17701 gagataattg gtaatggaaa tgccaagctc accagcaata tggtcatagc aggtgcgagc
    17761 gacttttaat tcctttttgt gaatgtgttg tttataactt ttgacatcac gctgaggaga
    17821 gtagaagctg agaaattcca gcatttctgc aatggcagca ctttttaaag aataataatg
    17881 aaaacgtcca taggtttgta gagtggttac ctgcgcttcg gtaagtaatt tgaggtgata
    17941 gctgacagta tgttgcttga ttttggtaaa gctggcaatt tcgttaaccg tgtgagcttt
    18001 tccatccatt agaatatcta aaatgtgcat ccgagaaggg tctgataaga gttttgtgat
    18061 gatgaaaata tctggttcca taaaaatttt ctccaaattt catatatcga tgatttt
//
LOCUS       DBERCA010000039        17393 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.039, whole genome shotgun sequence.
ACCESSION   DBERCA010000039 DBERCA010000000
VERSION     DBERCA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.039"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            161..1327
                     /locus_tag="ACL719_001447"
     CDS             161..1327
                     /locus_tag="ACL719_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS0699397.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1443..2480
                     /locus_tag="ACL719_001448"
     CDS             1443..2480
                     /locus_tag="ACL719_001448"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGS0699398.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2597..3810
                     /locus_tag="ACL719_001449"
                     /pseudo
     CDS             2597..3810
                     /locus_tag="ACL719_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            4262..9832
                     /gene="zmpC"
                     /locus_tag="ACL719_001450"
     CDS             4262..9832
                     /gene="zmpC"
                     /locus_tag="ACL719_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HGS0699399.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            10188..11042
                     /locus_tag="ACL719_001451"
     CDS             10188..11042
                     /locus_tag="ACL719_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0699400.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            11148..11705
                     /locus_tag="ACL719_001452"
     CDS             11148..11705
                     /locus_tag="ACL719_001452"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGS0699401.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            11922..12686
                     /locus_tag="ACL719_001453"
     CDS             11922..12686
                     /locus_tag="ACL719_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGS0699402.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            13299..14678
                     /locus_tag="ACL719_001454"
     CDS             13299..14678
                     /locus_tag="ACL719_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS0699403.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            14692..15357
                     /locus_tag="ACL719_001455"
     CDS             14692..15357
                     /locus_tag="ACL719_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGS0699404.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            15712..15885
                     /locus_tag="ACL719_001456"
     CDS             15712..15885
                     /locus_tag="ACL719_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS0699405.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTDLLRASQF"
     gene            15952..16362
                     /locus_tag="ACL719_001457"
     CDS             15952..16362
                     /locus_tag="ACL719_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS0699406.1"
                     /translation="MYYLPVDFYRYLIGREGQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            16478..>17393
                     /gene="pavB"
                     /locus_tag="ACL719_001458"
     CDS             16478..>17393
                     /gene="pavB"
                     /locus_tag="ACL719_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGS0699407.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT         5460 a         3050 c         3885 g         4998 t
ORIGIN      
        1 cagtgctttg agcagcctgc ggctagtttc ctacagattt tagttggaac tcgattcaat
       61 tcatgtgaca acgtgaaaat cgttagagca ttttatatag aatatacatg ggaatgtagc
      121 ttactcccat tcccatattt aatagaaaaa gaggaactca atgctacatt atacaaaaga
      181 agacttgctc gaattgggtg cagaaatcac tacgcgtgaa atctaccaac agcctgatgt
      241 atggagagaa gcttttgaat tttatcaagc aaaacgtgaa gaaattgcag ccttcctaca
      301 agaaatcgct gataaacatg actatattaa ggttatcttg acaggtgctg ggacttctgc
      361 ttatgtggga gataccttgc taccttattt taaggaagtc tatgacgaac gcaaatggaa
      421 tttcaatgct attgcgacaa cagatatcgt tgccaatcca gcaacctatt tgaaaaaaga
      481 tgtggcaact gtccttgtgt cttttgctcg tagtgggaat tcgcctgaaa gtttggcgac
      541 tgttgatttg gccaaatcct tggtggatga gctttatcaa gtgacgatta cttgtgcagc
      601 agatggtaaa ttggctcttc aagctcacgg tgatgatcgt aatctcttgc tcttgcaacc
      661 agctgtctct aatgatgctg gatttgccat gacttctagc tttacgtcta tgatgttgac
      721 aactctcttg gtctttgatc ctacagaatt tgctgttaag tctgaacgtt ttgaagttgt
      781 atctagtctt gcccgtaaag ttttagacaa ggcagaagat gtcaaagagc tcgttgattt
      841 agactttaac cgtgtcatct atctaggcgc tggtcctttc tttggacttg ctcatgaagc
      901 tcagctcaag attttggaat taactgctgg tcaagttgcg accatgtatg aaagcccagt
      961 tggcttccgt cacggtccaa aatctcttat caacgacaat acagttgttt tggtctttgg
     1021 tacaacgaca gactacactc gtaagtacga cttggacttg gttcgtgaag ttgctggtga
     1081 ccagattgct cgtcgtgttg tgcttttgag tgatcaagct tttggtcttg aaaatgtcaa
     1141 agaagtggcc cttggttgtg gcggtgtctt gaatgatatt taccgtgtct tcccttacat
     1201 cgtttatgcc caactctttg ctttattgac ttcactcaag gtagaaaata aaccagatac
     1261 accgtctcct acaggtacag taaaccgtgt agtacaaggt gtcataattc acgaatatca
     1321 aaagtaagac agtgtttatg aattcttgac aagaggattt gtaaattatc agataaacca
     1381 tagattgtca gtacgctttc tatggtttgt ttgcttgaga gaaatagtaa aaggagaaca
     1441 gaatgaaagc atacacagag cgtgtatttg gaaatgttga gggtgaggat gtcttggcct
     1501 atcgatttga gacagacggt ggctaccaac ttgaggttat gacttatggt gcgactatct
     1561 tgcgctatgt cgcacctgac aaggctggaa attttgccaa tgttatcttg ggatttgatg
     1621 actttgatag ttatgtaggc aatagtccca agcatggagc aagtgtaggt cctgtagcgg
     1681 gtcgtattgc aggtgcgacc tttgagctca atggtaagac ctatgacctt gaggttaata
     1741 atgctagcaa ctgtaatcac agtggttcaa ctggttggga ttccagcttg tttgaagttg
     1801 aagaagtaag cgatcatggc ttgactctct acacagagcg tacagatggg acaggagggt
     1861 tccctggaaa tctcaagatt tggatcagtt atcacttgga agaaactggt gcctatgaaa
     1921 tcagctacaa ggtaacgacc gatcaggata cgctggtcaa tccaaccaac cacagctatt
     1981 tcaacttgtc tggtgatttc acgcagacga ttgaccgtca tgtcttccaa ctaaacacag
     2041 agggcattta ctcaatcgct cctgacggtg ttcctgccaa aactccagaa gccaaccgtg
     2101 atgtggtcaa acacgtctac aatggtacct tgttgaagga tatctttgca gaagaagatg
     2161 agcaaatcca gctggcatca ggtttggatc atccatttgc ccttcctgca ggccatgaca
     2221 atgctggatt cctttatgac caaaattcag gtcgcttcct gcttttcaag acagaagctc
     2281 cttgctttgt ggtctacaca gcaaactttg tggatgaaag tgtcatcata ggaggtcagc
     2341 caatgctaca gcacaatggg attgctcttg aagcgcaagc tttaccagat gccattcaca
     2401 gtgaccttaa aggccaagtc attcttaaag ctggtcaaac cttcaccagt aagacacgtt
     2461 atgaacttgt tgtgaagtaa aagagtcatt gcgcctactt ttgggagcta ggaataggta
     2521 cgcagagaca aatagtagga aaatatgata taactaagcg ttgaaagcta tctgttaata
     2581 taatattcaa actacaataa ggagtaagaa agaaacgaag aaaattgtat ttgctagtgc
     2641 cttggctttg accttggctg gagcagtttt gacaaatgat gtttttgcga acgacagact
     2701 tgtggcaaca caaactactg atggtaaaaa tgaaaatgta ttgacctcag aggtgctaaa
     2761 accttctagt ggcaatgttt tggttggaat caaaggagaa tttgtggctc ctcatcaaca
     2821 atctattttg gatgccatca atgctatctg taaagaagcg gctgacgaag gtttggtaga
     2881 taagtatgtc cctatcaaat ggtcaactga cctagaaaag gcagcttttg ccagagctac
     2941 agaagcatct ataaccatgg atcatacccg tctttctagc aaagatcttt ggagtgcctt
     3001 tccaacttct aatagtataa tgggagaaaa tttggcatgg aatcatgacg gttttctaaa
     3061 agctattgaa caatggcgtg ctgaaaaagc agattatgtg gagaaaaaaa tagtggttca
     3121 gacaacggga aatctggtca ctatgagtcg ctaattaacc ctaaatttac acacatgggg
     3181 atggcagctt ttaaaaatcc taacaatcaa tacaaagcta ttacaattgc tcaaactcta
     3241 ggtgatgatg cttcttcaga ggaattggct ggtagatatg gttctgctgt tcagtgtaca
     3301 gaagtgactg cctcaaacct ttcaacagtt aaaactaaag ctacggttgt agaaaaacca
     3361 ctgaaagatt ttagagcgtc tacgtctgat cagtctggtt gggtggaatc taatggtaaa
     3421 tggtatttct atgagtctgg tgatgtgaag acaggttggg tgaaaacaga tggtaaatgg
     3481 tactatttga atgacttagg tgtcatgcag actggatttg taaaattttc tggtagctgg
     3541 tattacttga gcaattcagg tgctatgttt acaggctggg gaacagatgg tagcagatgg
     3601 ttctactttg acggctcagg agctatgaag acaggctggt acaaggaaaa tggcacttgg
     3661 tattaccttg acgaagcagg tatcatgaag acaggttggt ttaaagtcgg accacactgg
     3721 tactatgcct acggttcagg agctttggct gtgagcacaa caacaccaga tggttaccgt
     3781 gtaaatggta atggtgaatg ggtaaactag gctcaggcca taggtaaagc attcatctta
     3841 cttagcaaaa agaatgaacg ataagaaaga ggttgatggc gaacattggc ctcttttgat
     3901 ttataaagat tggattcttg tcgcctcaat ttcagacttt tctattgtaa gctaatattt
     3961 tatagcccat taaaagcata agcggtaatc taatttaaaa aatgctgtaa ttagtctgaa
     4021 gtccacactt acttgttgag atgttatctc tgttttttat cgttataatt tactgtattt
     4081 tttatagtat gcagaatatt tttaagtata tttcaataga aatttctatc gatttattgt
     4141 ataatgataa gtaattgttg aaaagtactc agaaaattcc atactatatt atttttatgt
     4201 ttatactttt atgctataaa atatagattg atataaagaa tatagaaaaa gcgaggttaa
     4261 tatgagccga aaaagcattg gtgagaaacg ccatagtttc tcgatgagaa agttgtcagt
     4321 gggattggta tcagttactg tatctagttt ctttttgatg agtcaaggga ttcaatcggt
     4381 atcggccgat aatatggaaa gtccaattca ttataagtat atgaccgagg gtaaattgac
     4441 agacgaggaa aaatccttgc tggtagaggc ccttccacaa ctggctgaag aatcagatga
     4501 tacttattac ttggtttata gatctcaaca gtttttaccg aatacaggtt ttaacccaac
     4561 tgttggtact ttccttttta ctgcaggatt gagcttgtta gttttattgg tttctaaaag
     4621 ggaaaatgga aagaaacgac ttgttcattt tctgctgttg actagcatgg gagttcaatt
     4681 gttgccggcc agtgcttttg ggttgaccag ccagatttta tctgcctata atagtcagct
     4741 ttctatcgga gtcggggaac atttaccaga gcctctgaaa atcgaaggtt atcaatatat
     4801 tggttatatc aaaactaaga aacaggataa tacagagctt tcaaggacag ttgatgggaa
     4861 atactctgct caaagagata gtcaaccaaa ctctacaaaa acatcagatg tagttcattc
     4921 agctgattta gaatggaacc aaggacaggg gaaggttagt ttacaaggtg aagcatcagg
     4981 ggatgatgga ctttcagaaa aatcttctat agcagcagac aatctatctt ctaatgattc
     5041 attcgcaagt caagttgagc agaatccgga tcacaaagga gaatctgtag ttcgaccaac
     5101 agtgccagaa caaggaaatc ctgtgtctgc tacaacggtg cagagtgcgg aagaggaagt
     5161 attggcgacg acaaatgatc gaccagagta taaacttcca ttggaaacca aaggcacgca
     5221 agaacccggt catgagggtg aagccgcagt ccgtgaagac ttaccagtct acactaagcc
     5281 actagaaacc aaaggtacac aaggacccgg acatgaaggt gaagctgcag ttcgcgagga
     5341 agaaccagct tacacagaac cgttagcaac gaaaggcacg caagagccag gtcatgaggg
     5401 caaagctaca gtccgcgaag agactctaga gtacacggaa ccggtagcga caaaaggcac
     5461 acaagaaccc gaacatgagg gcgaagcggc agtagaagaa gaacttccgg ctttagaggt
     5521 cactacacga aatagaacgg aaatccagaa tattccttat acaacagaag aaattcagga
     5581 tccaacactt ctgaaaaatc gtcgtaagat tgaacgacaa gggcaagcag ggacacgtac
     5641 aattcaatat gaagactaca tcgtaaatgg taatgtcgta gaaactaaag aagtgtcacg
     5701 aactgaagta gctccggtca acgaagtcgt taaagtagga acacttgtga aagttaaacc
     5761 tacagtagaa attacaaact taacaaaagt tgagaacaaa aaatctataa ctgtaagtta
     5821 taacttaata gacactacct cagcatatgt ttctgcaaaa acgcaagttt tccatggaga
     5881 caagctagtt aaagaggtgg atatagaaaa tcctgccaaa gagcaagtaa tatcaggttt
     5941 agattactac acaccgtata cagttaaaac acacctaact tataatttgg gtgaaaataa
     6001 tgaggaaaat actgaaacat caactcaaga tttccaatta gagtataaga aaatagagat
     6061 taaagatatt gattcagtag aattatacgg taaagaaaat gatcgttatc gtagatattt
     6121 aagtctaagt gaagcgccga ctgatacggc taaatacttt gtaaaagtga aatcagatcg
     6181 cttcaaagaa atgtacctac ctgtaaaatc tattacagaa aatacggatg gaacgtataa
     6241 agtgacggta gccgttgatc aacttgtcga agaaggtaca gacggttaca aagatgatta
     6301 cacatttact gtagctaaat ctaaagcaga gcaaccagga gtttacacat cctttaaaca
     6361 gctggtaaca gccatgcaaa gcaatctgtc tggtgtctat acattggctt cagatatgac
     6421 cgcagatgag gtgagcttag gcgataagca gacaagttat ctcacaggtg catttacagg
     6481 gagcttgatc ggttctgatg gaacaaaatc gtatgccatt tatgatttga aaaaaccatt
     6541 atttgataca ttaaatggtg ctacagttag agatttggat attaaaactg tttctgctga
     6601 tagtaaagaa aatgtcgcag cgctggcgaa ggcagcgaat agcgcgaata ttaataatgt
     6661 tgcagtagaa ggaaaaatct caggtgcgaa atctgttgcg ggattagtag cgagcgcaac
     6721 aaatacagtg atagaaaaca gctcgtttac agggaaactt atcgcaaatc accaggacag
     6781 taataaaaat gatactggag gaatagtagg taatataaca ggaaatagtt cgagagttaa
     6841 taaagttagg gtagatgcct taatctctac taatgcacgc aataataacc aaacagctgg
     6901 agggatagta ggtagattag aaaatggtgc attgatatct aattcggttg ctactggaga
     6961 aatacgaaat ggtcaaggat attctagagt cggaggaata gtaggatcta cgtggcaaaa
     7021 cggtcgagta aataatgttg tgagtaacgt agatgttgga gatggttatg ttatcaccgg
     7081 tgatcaatac gcagcagcag atgtgaaaaa tgcaagtaca tcagttgata atagaaaagc
     7141 agacagattc gctacaaaat tatcaaaaga ccaaatagac gcgaaagttg ctgattatgg
     7201 aatcacagta actcttgatg atactgggca agatttaaaa cgtaatctaa gagaagttga
     7261 ttatacaaga ctaaataaag cagaagctga aagaaaagta gcttatagca acatagaaaa
     7321 actgatgcca ttctacaata aagacctagt agttcactat ggtaacaaag tagcgacaac
     7381 agataaactt tacactacag aattgttaga tgttgtgccg atgaaagatg atgaagtagt
     7441 aacggatatt aataataaga aaaattcaat aaataaagtt atgttacatt tcaaagataa
     7501 tacagtagaa tacctagatg taacattcaa agaaaacttc ataaacagtc aagtaatcga
     7561 atacaatgtt acaggaaaag aatatatatt cacaccagaa gcatttgttt cagactatac
     7621 agcgataacg aataacgtac taagcgactt gcaaaatgta acacttaact cagaagctac
     7681 taaaaaagta ctaggagcag cgaatgatgc agccttagat aacctatact tagatagaca
     7741 atttgaagaa gttaaagcta atatagcaga acacctaaga aaagtattag cgatggataa
     7801 atcaatcaat actacaggag acggtgtagt tgaatacgta agtgagaaaa tcaaaaataa
     7861 caaagaagca tttatgctag gtcttactta tatgaaccgt tggtacgata ttaattatgg
     7921 taaaatgaat acaaaagatt tatctacgta caagtttgac tttaacggaa ataatgagac
     7981 ttcaacgttg gatactattg tcgcattagg aaatagtgga ctagataacc tgagagcttc
     8041 aaatactgta ggtttatatg cgaataaact tgcatcggta aaaggagaag attcagtctt
     8101 tgacttcgta gaagcgtata gaaaactgtt cttaccaaac aaaacaaata acgagtggtt
     8161 taaagaaaat acaaaggcat atatagtcga aatgaagtct gatattgcag aagtacgaga
     8221 aaaacaagaa tcaccaacag ccgatagaaa atattcatta ggagtttacg atagaatatc
     8281 agcaccaagt tgggggcata agagtatgtt attaccacta ctaactttac ctgaagaatc
     8341 tgtgtatatt tcatcgaata tgtctacact tgcattcggt tcgtatgaaa gatatcgtga
     8401 tagtgtggat ggagttattc tttcaggaga tgctttacga acttatgtaa gaaatagagt
     8461 tgatatagca gcgaaaaggc atagagacca ttatgatatt tggtacaatc ttcttgacag
     8521 tgcttcaaaa gaaaaacttt tccgttctgt gatagtttat gatggattca atgtaaaaga
     8581 tgagacagga agaacttatt gggcaaggtt aacggataaa aacatcggct ctattaaaga
     8641 attcttcgga cctgttggga aatggtatga gtataatagt agtgcaggag cgtatgcgaa
     8701 tggaagttta acgcactttg tgttagatag attattagat gcttatggaa cgtcggttta
     8761 tactcatgaa atggttcata attctgattc tgcaatctac tttgaaggaa atggtagacg
     8821 tgaaggattg ggagcggagt tatacgcact tggtttactg caatctgtag atagtgtaaa
     8881 ttctcatatt ttagctttaa atacgttata taaagcagaa aaagatgatt tgaatagatt
     8941 gcatacatat aatccggtgg aacgtttcga ttcggatgag gcgcttcaaa gttatatgca
     9001 tggatcatat gatgtaatgt atacacttga tgcgatggaa gcaaaagcga tattagctca
     9061 aaataatgat gttaagaaaa aatggtttag aaaaatagaa aattattacg ttcgtgatac
     9121 tagacataat aaagatacac atgcaggaaa taaagtccgt ccattaacag atgaagaagt
     9181 agctaactta acatcgttaa actcattaat cgacaacgac atcataaata gacgtagcta
     9241 tgatgataat agagaatata aacgaaatgg ctactatact ataagtatgt tctctcctgt
     9301 atacgcagcg ctaagcaatt cgaaaggtgc tcctggagat attatgttta gaaaaatagc
     9361 ttatgaatta cttgcggaaa aaggttatca caaaggattc ctaccttatg tttctaatca
     9421 gtacggagca gaagcatttg ccagcggaag caaaacattc tcatcatggc atggaagaga
     9481 tgttgcttta gtgacagatg atttagtatt taagaaagta ttcaatggtg agtactcatc
     9541 atgggctgat ttcaaaaaag caatgtttaa acaacgtata gataaacaag ataatctgaa
     9601 accaataaca attcaatacg aattaggtaa tcctaatagt acaaaagaag taactataac
     9661 aacggctgca caaatgcaac aattaattaa tgaagcggct gcgaaagata ttactaatat
     9721 agatcgtgca acgagtcata ccccagcaag ttgggtgcat ttattaaaac aaaaaatcta
     9781 taatgcatat cttcgcacta cagatgactt tagaaattct atatataaat aagattgtag
     9841 agtttcattg ttgagtagtg tttcttgtaa ggatgaggag tcagatgaca aatcgactcc
     9901 tttttcttat ggatcgatgt agagatttga ttgaatgcag attgcaggaa tcatcttcaa
     9961 ctcatcaacg accaatggtg acaaggtgga tttcaatccc acagaaaatg ttgatttgag
    10021 aaataacttt gctagtctag taaaataaat acaaaacaat cctagaagat tttttctggg
    10081 attgtttttt gctgagtggg atgcttcaag ttgtctggct tgactttctt gagggaagtt
    10141 atataatagt tgtaataatt aaagttacaa taaaagagga aatatttatg acatatgaat
    10201 accagagcca catttattta gcagaggcag ttttaaatgt gaaggatttg gtcagtcaaa
    10261 cagtttttta tcagcagatt attggtttag aaatcctatc tcaaacggat acagaggtcg
    10321 ttctgggact tggaggaaaa gccttggtac acttgattca agcacaagag ggtggagaag
    10381 taagggaaca ttatggtctt taccatctgg ctattctttt gccgacacga aaggctttgg
    10441 cggatgtctt gaagcacctg acggatttac agattcctct tgttggcggt gcagatcacg
    10501 gttacagtga ggccctttac ttagaggact tggagggaaa tggcattgaa ctctatcgag
    10561 ataagccagt ttccacatgg gatattcgag aagatggacg tattatcggg gtgactgaag
    10621 tccttgcggc tcaggatatc tatgagttgg gggaaagagt agagcctttt atcctagcag
    10681 agggtacgag aatggggcat attcatcttt ctgtcaagga tagtcgaaag tccagacagt
    10741 tttatcaaac ggtgttaggg ctcgaggata aattcagtgt gcctagtgct agttggatcg
    10801 cagctgggga ctaccatcat catttagcag tcaacgaatg gggaggaaaa ggtctggatc
    10861 cgcgtaaaca agtcctacca ggtttagcct actatgtcat cgaagtcgca cataaagaag
    10921 aactgttaac gattgcccaa cgagcacaag aagttgacgc accaatcaaa tggatgacat
    10981 cgatccaatt ggaaatcaca gactcagatg gcatcgtgac ccgtattcgt ttagctagat
    11041 agatggtatg tgatgaaggt agagcatcaa ttgtagaaag tgctccttat tctagtcaga
    11101 tacgtggaaa atttgatatg atagtaggga ataaggagaa gaataatatg actagcgaat
    11161 accagaaaat gatagcagga gagttttacc gtccatcaga cccagaactg agagccttag
    11221 ctcaggcttc tcgccaaaaa caggccgcct ttaacaagga agagaacccc ttgaagggag
    11281 ccgaaatcat caagacttgg tttgcctcaa ccgggaaaaa tctttacatc aacactcgct
    11341 tgatggtgga ctacggtgtc aacatccatc taggggaaaa tttttattct aattggaact
    11401 tgaccatgct ggatatctgt cccattcgta tcggggacaa tgctatgatt ggtcctaatt
    11461 gtcagttttt gacacccctc catccactag atccacagga acgcaattca ggtatcgagt
    11521 acggaaagcc tatcacaatc ggagataatt tctggactgg tggtggcgtc attgtccttc
    11581 ctggagtgac actgggaaat aatgtcgttg caggagcagg ggcagtaatt accaaatctt
    11641 ttggcgacaa cgttgtccta gctggcaatc ctgcgcgcgt gattaaggaa atacctgtta
    11701 aatagaagta aaaaggaaca gctggggttg tttctttttt gtaggtttca tcatttttta
    11761 cccagttcac atttacctac tctatctctt agcaagtctg tttcattaag caagttcaaa
    11821 gcatctcgta agtgggatgt ttttctcctc agttcatcag cttcctcctt gacactcggt
    11881 cagattttga tacaatagta caaaattaga ggaggcaggc tatgattcag aaacatgcga
    11941 ttcctatttt agagtttgat gacaatcctc aggcggttat catgcccaat cacgaggggc
    12001 tggacttgca gttgccaaag aagtgtgttt atgcattttt aggtgaggag attgaccgct
    12061 atgcgaggga agtaggggcg aactgtgttg gcgaatttgt ttctgccacc aagacctatc
    12121 cagtttatgt cgtgaactac aaggacgagg agatttgtct ggttcaggct cctgtcggtt
    12181 cagctccagc agcccagttt atggattggt tgattggcta tggtgtggag cagattatct
    12241 ctactgggac ctgtggtgtc ctagctgata tagaggaaaa tgcctttcta gtccctgttc
    12301 gcgctctgcg agatgaagga gccagttacc actatgtggc accttctcgt tatatggaaa
    12361 tgcagccaga ggctattgct gccattgagc gagttttgga tgatagaggg attccttatg
    12421 aagaagtcat gacctggtcc acagatggtt tttaccgaga aacggctgaa aaggtggctt
    12481 atcgcaagga agaagactgt gctgttgtgg agatggagtg ttccgctctt gcggcagtag
    12541 ctcaattgcg tggggttctc tggggtgaat tgttgttcac agcagattct ctagcggact
    12601 tggaccagta cgacagtcgt gactggggct cggaagcttt taataaggcg ctagaactga
    12661 gtttagcaag tgttcaccac ctttagttgt actggcaaag gatttgtttt atcataaaat
    12721 gtctagctca tacttttcaa aaatatgttt aaacgaggtc accttcctct tgtcctaggc
    12781 atgttgaggt tgggaaaaat ctttaaaatc agaaaaacgt atcatatcag gtgatgaaaa
    12841 ctttgacact atgcgtttta tgtcgataag atttagagtg agatgaaatg atactcttcg
    12901 aaaatctctt caaaccaggt cagcttcacc ttgccgtagg tatatgttac tgacttcgtc
    12961 agtcttatcc ggcaacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatttgcaa
    13021 cctcaaaaca gtgttttgag caacctgtga ctagctttct aatcgatgcc ttggttttca
    13081 ttgcctataa tcaaaaagag aaattttctc ctgaaaagca tatagagtag ctggcgttaa
    13141 aagctcctgt cttgcttttt tgacctatag tcacatctat caagtattgt tcttgcctaa
    13201 gctatcaata aaaaggtggc attttttagg cttggtgtta gtagattttg ccttatccta
    13261 tctaagtcat ttcgaacttt ttatggtaca atggaaacat gttattcaaa ttatctaagg
    13321 aaaaaataga gctaggctta tctcgtttat cgccagcccg tcgtattttt ttgagttttg
    13381 ccttggtcat tttactaggc tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct
    13441 cacgagcgac ttattttgat catcttttca ctgctgtatc tgcagtctgt gtgacgggtc
    13501 tctcaaccct tccagtagct cacacctata atatctgggg tcaaataatc tgtttgctct
    13561 tgattcagat cggtggtcta gggctcatga cctttattgg ggttttctat atccagagca
    13621 agcaaaagct tagtcttcgt agccgtgcaa ctattcagga tagttttagt tatggagaaa
    13681 ctcgatcttt gagaaagttt gtctattcta tttttctcac gacctttttg gttgagagct
    13741 tgggagctat tttgcttagt tttcgcctta ttcctcaact tggctgggga cgtggtcttt
    13801 ttagttccat ttttctagcg atctcagcct tctgtaatgc cggttttgat aatttaggga
    13861 gcaccagttt atttgctttt cagaccgatt tactggtcaa tctggtgatt gcaggcttaa
    13921 ttattacagg cggccttggt tttatggtct ggtttgattt ggctggtcat gtaggaagaa
    13981 agaaaaaagg acgtctgcac tttcatacga agcttgtact attgttgact ataggtttgt
    14041 tgttatttgg aacggcaact actctctttc ttgagtggaa caatgctgga acgattggca
    14101 atctccctgt tgccgataag gttttagtta gcttttttca aacagtgacg atgcgaacag
    14161 ctggcttttc tacgatagat tatactcagg ctcatcctgt gactcttttg atttatatct
    14221 tacagatgtt tctaggtggg gcacctggag gaacagctgg gggactcaag attacgacat
    14281 tttttgtcct cttggtcttt gcacgaagtg agcttctagg cttgcctcat gccaatgttg
    14341 cgagacgaac gattgcgccg cgaacggttc aaaaatcctt tagtgtcttt attatctttt
    14401 tgatgagctt cttaatagga ttgattctgc tagggataac agccaaaggc aatcctccct
    14461 ttatccacct catatttgaa accatttcag ctcttagtac agttggtgta acggcaaatc
    14521 tgactcctga ccttgggaaa ttggctctca gtgttatcat gccacttatg tttatgggac
    14581 gaattggtcc cttgaccttg tttgttagct tggcagatta ccatccagaa aagaaagata
    14641 tgattcacta tatgaaagca gatattagta ttggttaaga aaggaaagag catgtcagat
    14701 cgtacgattg gaattttggg cttgggaatt tttgggagca gtgtcctagc tgccctagcc
    14761 aagcaggata tgaatattat cgctattgat gaccacgcag agcgcatcaa tcagtttgag
    14821 ccagttttgg cgcgtggagt gattggtgac atcacagatg aagaattatt gagatcagca
    14881 gggattgata cctgcgatac cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg
    14941 cttgcggtta tgcactgtaa gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt
    15001 cagaccgcta agaaagtgct agaaaagatt ggagctgact cggttatctc gccagagtat
    15061 gaaatggggc agtctctagc acagaccatt cttttccata atagtgttga tgtctttcag
    15121 ttggataaaa atgtgtctat cgtggagatg aaaattcctc agtcttgggc aggtcaaagt
    15181 ctgagtaaat tagacctccg tggcaaatac aatctgaata ttttgggttt ccgagagcag
    15241 gaaaattccc cattggatgt tgaatttgga ccagatgacc tcttgaaagc agatacctat
    15301 attttggcag tcatcaacaa ccagtatttg gataccctag tagcattgaa ttcgtaaaga
    15361 gggatgaccc ctcttttttg atgcccaaga tggcaaatag agacagaagc cccttgtctt
    15421 ctagtaaaag ttcttcaaag gctggacttt atggtaaaat agaaggaagt gacaagagag
    15481 agtaatactc aatgaaaatc aaagatcaaa ctaggaaact agctacgggc tgcttaaaac
    15541 actgttttga ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg
    15601 cagatagaac tgacgaagtc agtaacatct atacggcaag gcgatgttga cgcggtttga
    15661 agagattttc gaagagtata agaaaaaatc agtcccctaa aggagcagat tatgaagtta
    15721 ttgtctatcg ccatttctag ctataatgca gcagcctatc ttcattactg tgtggagtcg
    15781 ctagtgattg gctgggacca ggtcggaaat ttctccaaag gccagtatat catgatgcac
    15841 tcgctgattt atcggacaga tttgttgcgt gctagccagt tctaattgcc tgaacatact
    15901 ttttatgtcg ataatctctt tgtctttacg ccccttcagc aggtcaagac catgtactat
    15961 ctgcctgtcg atttctatcg ttatttgatt gggcgtgagg gccagtctgt caatgagcaa
    16021 gtgatgatta agtgcattga ccagcaactc aaggtcaatc gactcttggt agaccagctt
    16081 gatttgtccc aagtgagtca tcccaaaatg cgagaatatc tgctgaatca tattgaaatc
    16141 acgacggtga tttccagtac cctgctcaac cgatctgaaa cagcggagca tctggcaaaa
    16201 aaacgccaat tgtggaccta tattcagcag aaaaatccag aagtctttca ggctattcgt
    16261 aagaccatgt tgagccgttt gaccaaacat tctgtcttgc cagatcgcaa actgtccaat
    16321 gtcgtctatc aaatcaccaa atctgtttat ggatttaatt aatataagtg ttttataaga
    16381 gggatttaag aaaaatttta actttttctt agtccttttt aatttcagga gattatacta
    16441 gagtcatcaa ataaagaaag actctaagga gaatcctatg aaattcaatc caaatcaaag
    16501 atatactcgt tggtctattc gccgtctcag tgtcggtgtt gcctcagttg ttgtggctag
    16561 tggcttcttt gtcctagttg gtcagccaag ttctgtacgt gccgatgtgg tcaatccgac
    16621 cccaggtcaa gtcttacctg aagagacatc gggaacgaaa gagggtgact tatcagaaaa
    16681 accaggagac accgttctca ctcaagcgaa acctgagggc gttactggaa atacgaattc
    16741 acttccgaca cctacagaaa gaactgaagt gagcgaggaa acaaactctt ctagtctgga
    16801 tacacttttt gaaaaagatg aagaagctca aaaaaatcca gagctaacag atgtcttaaa
    16861 agaaactgta gatacagctg atgtggatgg gacacaagca agtccagcag aaactactcc
    16921 tgaacaagta aaaggtggag tgaaagaaaa tacaaaagac agcatcgatg ttcctgctgc
    16981 ttatcttgaa aaagctgaag ggaaaggtcc tttcactgcc ggtgtaaacc aagtaatccc
    17041 ttatgaacta ttcgctggtg atggtatgtt aactcgtcta ttactaaaag cttcggataa
    17101 tgctccttgg tctgacaatg gtactgctaa aaatcctgct ttacctcctc ttgaaggatt
    17161 aacaaaaggg aaatacttct atgaagtaga cttaaatggc aatactgttg gtaaacaagg
    17221 tcaagcttta attgatcaac ttcgcgctaa tggtactcaa acttataaag ctactgttaa
    17281 agtttacgga aataaagacg gtaaagctga cttgactaat ctagttgcta ctaaaaatgt
    17341 agacatcaac atcaatggat tagttgctaa agaaactgtc gaaaaagctg tta
//
LOCUS       DBERCA010000040        16856 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.040, whole genome shotgun sequence.
ACCESSION   DBERCA010000040 DBERCA010000000
VERSION     DBERCA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.040"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..277)
                     /locus_tag="ACL719_001459"
                     /pseudo
     CDS             complement(1..277)
                     /locus_tag="ACL719_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(323..556)
                     /locus_tag="ACL719_001460"
     CDS             complement(323..556)
                     /locus_tag="ACL719_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS0699408.1"
                     /translation="MKEKEIFDSIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(562..1554)
                     /gene="plsX"
                     /locus_tag="ACL719_001461"
     CDS             complement(562..1554)
                     /gene="plsX"
                     /locus_tag="ACL719_001461"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGS0699409.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETEVVAQTAREFSGE"
     gene            complement(1551..2321)
                     /gene="recO"
                     /locus_tag="ACL719_001462"
     CDS             complement(1551..2321)
                     /gene="recO"
                     /locus_tag="ACL719_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGS0699410.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2318..3487)
                     /locus_tag="ACL719_001463"
     CDS             complement(2318..3487)
                     /locus_tag="ACL719_001463"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0699411.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTSQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3636..4646
                     /locus_tag="ACL719_001464"
     CDS             3636..4646
                     /locus_tag="ACL719_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGS0699412.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(4829..5266)
                     /locus_tag="ACL719_001465"
     CDS             complement(4829..5266)
                     /locus_tag="ACL719_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGS0699413.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5351..7984)
                     /gene="polA"
                     /locus_tag="ACL719_001466"
     CDS             complement(5351..7984)
                     /gene="polA"
                     /locus_tag="ACL719_001466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGS0699414.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8240..9147
                     /locus_tag="ACL719_001467"
                     /pseudo
     CDS             8240..9147
                     /locus_tag="ACL719_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            9273..9554
                     /locus_tag="ACL719_001468"
                     /pseudo
     CDS             9273..9554
                     /locus_tag="ACL719_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9688..10656)
                     /locus_tag="ACL719_001469"
     CDS             complement(9688..10656)
                     /locus_tag="ACL719_001469"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS0699415.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(10801..11615)
                     /locus_tag="ACL719_001470"
                     /pseudo
     CDS             complement(10801..11615)
                     /locus_tag="ACL719_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(11640..12137)
                     /locus_tag="ACL719_001471"
     CDS             complement(11640..12137)
                     /locus_tag="ACL719_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGS0699416.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(12210..13571)
                     /gene="radA"
                     /locus_tag="ACL719_001472"
     CDS             complement(12210..13571)
                     /gene="radA"
                     /locus_tag="ACL719_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGS0699417.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLQQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(13585..14100)
                     /locus_tag="ACL719_001473"
     CDS             complement(13585..14100)
                     /locus_tag="ACL719_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0699418.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(14102..14545)
                     /locus_tag="ACL719_001474"
     CDS             complement(14102..14545)
                     /locus_tag="ACL719_001474"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGS0699419.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(14605..14698)
                     /gene="ffs"
                     /locus_tag="ACL719_001475"
     ncRNA           complement(14605..14698)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL719_001475"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(14731..15198)
                     /gene="tadA"
                     /locus_tag="ACL719_001476"
     CDS             complement(14731..15198)
                     /gene="tadA"
                     /locus_tag="ACL719_001476"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGS0699420.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(15399..16685)
                     /locus_tag="ACL719_001477"
     CDS             complement(15399..16685)
                     /locus_tag="ACL719_001477"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGS0699421.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         5001 a         4161 c         2983 g         4711 t
ORIGIN      
        1 atgattgata ataccagcaa tcaaattcat tcgtaatccg aagcgtttac gatgatttcg
       61 ataggttgtt gaaaacattt taaacgtttt tactttggca aagatattct caaccttgct
      121 tctctcctta gatagcgcat gattacaggc tttatcttca actgttagcg gcttgagttt
      181 gctggattta cgtggaagtt tgtgcttgag gatatatctt catgagccct tgataaccac
      241 tgtcagccaa gattttacca gcttgtccga tatttctcat ttctaaaaac catctacttc
      301 cgttgacttg gaactccgat tgctattttc cttgaatgat tttaaccaca tctcctacac
      361 tttggagttg gtcaatttcc tcatcgctga tttcgatact aaattcatcc tccagcgtca
      421 agataaactc catcaaatca actgagtcag catccaagtc gtctttcaga ctcaaggatt
      481 ctgtcacgac aaagtcctct ccctgtcgct cttggataat ggtcacaata ctgtcaaaaa
      541 tttctttttc tttcatctct tttattctcc tgaaaattca cgcgcagtct gggcaactac
      601 ttctgtttct agcatggtac gaatctggcg aatcgtacta taaacagcct tggcatcgct
      661 tgagccatga gtcttgacaa caggtgcctt gacaccaaac aagaccgctc caccaacatc
      721 tgaataattg agctgttttt tcaaacctct gaggctgtcc ttgagaagga gggcacctag
      781 tttcgctcga agaccaccac ctgtaatagc tgtcttgagc aagcccatga ttcccatagc
      841 tgtcccttcg atggatttga gcacagcgtt tcccgtgaaa ccatctgcca caacaacatc
      901 tgcaacacca ttcatcaaat cacgcgcttc cacgtttccg ataaagttca aactttcatc
      961 agccgccagt aattcataag tttccttacg aagcgggtcg cccttgctac tctctgttcc
     1021 gttgttgagc aaaccaacac gtggttgcgc aatgccacga acattcttgg catagaaaga
     1081 acctaggaca gcgtattgat ggaggtgctg ggctgtattt tctgcattag caccgaggtc
     1141 aagcatgtca aaacctttcc catctacagt cggcaatgtt gacataagtc caggacggtc
     1201 gatattcttg atacgaccca cgatgaagaa tccagcagcc aacaaagcac ctgtattccc
     1261 agccgaaagg acagcgtctg cttcaccatc tttgacagcc ttggctgcca ataccatact
     1321 ggcatttttc ttattccgaa tagctctcgt aggttcatca tccgaatcaa tcttctcatc
     1381 cgtatggata atgctgacgc gctctgtcgc tgtcagatat tgcttgattt tagcttcatc
     1441 tccgtagagt tgaacctcga tatctgaaaa gtcagatagg gcttgattga caccctcaac
     1501 gatggcctga ggtgcgtaat cgccccccat ggcatctact gcgatttttt tcatttcttt
     1561 tcctctttta gtaattgtcc ccagtctgct agggaatcaa taaatttctt tgattttagg
     1621 tgaatcccaa cgtactcttc atatagttga tccataaatt ggcgtagctc ttgcttgatt
     1681 ccaggcttga gcgaaatggt ctccaaagtc tcaaaatcaa tagcttgaaa ttgattgagc
     1741 agatagggga tattgggatt gagatggcaa cgtctctcat cctcatgata atgctctgga
     1801 cagagacagg ctccatattt gaaagaaaag tcaaaagcct gaccaacccg atggcagaag
     1861 acacactcat taaaattgag gctgattcca aatcgagtca aaatttgaat ttcaaaaata
     1921 ttggtcaaaa cctgataatc caagcctgct tccatcaact ccaaagtctt ttgcaaaaaa
     1981 gcaaacaagg gagcatcctg ctgattgtcc tgcaaactag catctgcaag agctgccaca
     2041 taggtcgcat aggccatgac aaagaggtca ctattaatct tgggaaaagt catgacctca
     2101 tgatagtctt cgatgtaact gagtccgtca tcattgattc gcaagagaaa tcgtgccagc
     2161 accaagggct gaataacagg cgccagctta gactgaccag cgtgtttgac aaaaaacatg
     2221 catttgccaa cctgctctgt aaaaattttg acaagcttgt catcctcacg aaaattgcga
     2281 ttgtaaagca ccaagccttg actcgtgata gactgaatca tgcttctctc atatactcct
     2341 caagtcgttt catggcttct ttgatagtct ccatgctggc tgcataagat aggcggacgt
     2401 agccttcccc gtaacgtcca aaggctgcac cagggataaa ggcaacggcc ttcttctgag
     2461 caaaatcctt cagaaaagca aaggagtctt gattgtagcc cgctggaatt ttagcaaaaa
     2521 tatagaaggc accgtctggt ttgataatct caaaaccaag agcagtcatt ttttcgatga
     2581 tatagtcccg acgctggata tattccttct tcatgggctc cgcatcattt ttaccagccg
     2641 tcaaggcttc taccgcagca tgttgcgcca tggtatttgc ggcagtgacc aagtattggt
     2701 gactcttgat taactgggat gtgaaggccg caggagcgaa aatcagcccc aaacgccaac
     2761 ctgtcatggc atgcgattta gacaaaccat tgataataat agcctggtct ctcaacatcg
     2821 ttcctagaga cacatgggct tcgcctgtgt aggtcaattc tgagtaaacc tcatcacaga
     2881 caacaaaaat ttcgtacttg cgtaaaacag ctgccaaggc ctctaactgc tctcgactgt
     2941 aggtaattcc tgtcggattg gctggatagt tgagaataac cgccttgagc ttatcaccct
     3001 gctccaaaat ggccttctcc aacatctcag gagtcaagac aaaaccattt tcagtcgtat
     3061 caatctcaac aatttctgcc ccaactaagt taacaatcgg ttcatagcct ggataagcag
     3121 gagctggcaa aagtaccttg tctccctctt ccaaaatagc cgtcaaagtc gcagataaag
     3181 cctctgtcgc cccaattgta accaagattt cattttcagg agcatagtcc agttggtact
     3241 tttccttaac aaagtcacta gctgcctgac gtagagtcag cagaccactc atccctgtat
     3301 agtaggattg gttctgatca atcgctcgct tggccgcctc cttgacatgg tctggcgttg
     3361 taaaatcagg ttcccccaag gtcaaacgca agaccccagg aatctccgaa atagcctggt
     3421 caaactgacg aatcaacgaa acttgaattt tatctaactg tttattaaag cgcttagtta
     3481 agtccataga tacctcctac tttcaaaacg tatctctatt atactacaaa agccccccga
     3541 ccgcaaaaat acattagagg atagctttcc actttctatt ttacttttcc tgtttatagg
     3601 tctataatgg taatagattt tatttggagg tttttatgtc atttctatca aaaaatggag
     3661 caggtatctt ggcctgcctt ctcatttcta tcgtatcttg gtacttagga ggattcttcc
     3721 ctgtgattgg cgcgcccgtt tttgccattt tcataggcat gctcctacat ccctttctct
     3781 cgtcctataa acaactggat gctggtttga cctttagttc caagaagttg ctccaatatg
     3841 ccgttgtctt gcttggtttt ggtctcaata tctcgcaggt cttcgcagtt ggccaatctt
     3901 cactccctgt catcctgtcc actatctcaa tagctctgat tattgcctac ctcttccagc
     3961 gtttctttgc cctggataca aaactggcta ccttggttgg agtaggttct tctatctgtg
     4021 ggggttctgc cattgcagcg acagcgcccg ttattgatgc taaggaaaag gaagtagccc
     4081 aagccatttc cgttatcttt ttcttcaatg tcttggctgc gctcatcttt ccaaccctcg
     4141 gcacctggct tcatctatcc aatgaaggct tcgccctctt tgcagggact gcggtcaacg
     4201 acacttcctc tgtaacggct gccgccagcg cttgggacag tctttaccaa agcaataccc
     4261 tcgagtctgc aaccattgtt aaactcacac gtactttggc cattatccct atcacgctct
     4321 ttctatccta ctggcaaagt cgccaacaag aaaacaagca aagcctgcaa ctgaaaaaag
     4381 tcttcccact ttttatcctt tactttatcc ttgcctctct cctcactaca ctactcacct
     4441 ctctaggtgt gtccagtagt ttctttactc ctctcaaaga actctctaaa ttccttattg
     4501 tcatggccat gagtgctatc ggtctcaaaa ccaatctggt cgctatggtc aaatccagtg
     4561 gaaaatccat tcttcttgga gccatctgct ggattgccat catcctcacc actcttggta
     4621 tgcagaccct tatcggcatt ttctaacact cttcgaaaat ctcttcaaac cacgtcaacg
     4681 tcgccttgcc gtatatatgt tactgacttc gtcagttcta tctgcaacct caaaacggtg
     4741 ttttgagcag cccgcggcta gtttcctagt ttgctctttg attttcattg agtataacac
     4801 aaaaggtagc ccatcagcta cctttttctt atgcttcctc aatcaagcga gtatgttctc
     4861 tcttgataca gcgattcatc acgatatcat cacatccacc atcacgcaaa atctctttcg
     4921 cttctaaact ttcaagtcct agctgtgccc aaaaaatctt ggcatcagct ttgagaaaat
     4981 cacgcgccac atcgggcaga aattcactgc gacgataaac attgacaata tctacaggaa
     5041 aaggaatttc agcgaggcta gcataagcct tttcacccaa gatttcgcca cctgccgcct
     5101 tgggattgac tgggatgatt ttatagcccc gagcctgcat ttcctttgtt actcgattgc
     5161 tggttgtttc ttcacggtca gacaaaccca ccacagcaag ggttttactc gttgcgagat
     5221 actgacgaat cacgccatca cttggattga taaattcttg actcatagaa atcctccttt
     5281 ttcatcagta tagcacattt tgaaaaggtt tgcagaatta tactacaaaa aaggaggact
     5341 agcccccttt ttatttagcc tcgtaccagg ttgccccttc attctcatct gcgataagag
     5401 gaacactgag ttgaatggct tcttccatgg tttgtttcac caattttttc atctctacca
     5461 attcagattt aggcacttca aggacgattt catcgtgcac ttgtaacagc atcttagtct
     5521 gataaccacc tgcaaccaag gctttatcca gctgaatcat ggcaatcttg agaatatctg
     5581 ctgccgaacc ctggataggt gagttgatag cagttcgttc cgcaaaacca cgaatattga
     5641 agttgcgcga attgatatct ggcaactcac ggcgacgctt aaagagggtc tctacatagc
     5701 ccttatcacg cgcctcccgc accacttcat ccatgtagtt tttaatacct ggaaaacgtt
     5761 caaagtaggt atcaatgtag gctttggctt ccttacgact aattcccaaa ttattagaca
     5821 agccaaagtc tgaaatccca taaaccactc caaagttaac tgccttggca ttgcgacggt
     5881 cgtttgcagt cacatcatca ggacgctcaa tgccaaagac ccgcatggct gtcgaagtat
     5941 ggatatctgc cccctcttgg aaggccttaa tcaagtgctc atccttagaa atatgcgcca
     6001 aaacgcgcaa ttcaatctgt gaatagtcag agctgagtag cacactatcc tcccactctg
     6061 gcacaaaagc cttccgaatc aagcgcccct gttccaatcg ggcaggaata ttttgcaagt
     6121 ttggatccac actagacaaa cgcccggtct gggtcaaatc ctgcacatag cgagtatgaa
     6181 tctttccatc agccaaaatc cagtcctgca agccaattac ataagtagat tgaatcttag
     6241 caatttgacg gtaatccagg attttcttaa caatcggagc aataggagcg agacgctcca
     6301 aaacatccac tgctgtcgaa taacctgtct tggttttctt agtgtattct agaggaagtc
     6361 ccaatttctc aaagagaagc acgcccaact gcttaggcga gttgacatta aactcctcac
     6421 cagccagctc gtaaatctct tgagtcagtt tttcaatgac aagctcattt tcagcctgca
     6481 tctcaagcaa ggtctctttc ttgaccataa tcccagcaat ttccatcttg gcaaggacaa
     6541 aagccagagg ttgctccata tcataaagaa gctctaattg cccattttcg ctgagttttt
     6601 caagtaaaat aggctctgtt tctaccaaaa cagcaagttt acaagctaag tgttccaaga
     6661 atttctcacg ttcaggaatg gcctttttaa cacccttacc gtagaaagtt tcatcatcaa
     6721 ccaagtaagt ctgaccataa agactagcga tggtcgcaat ttcattgtcc tccacagtcg
     6781 aaaggaggta tttagccaaa cggatgtcaa aagcaggcgc ctgcaaatcc acaccaaaac
     6841 gttgcaaaag aactttaacc ttcttaaagt cataaactct cagagatgtt ttttctaaga
     6901 aatccttgaa aatcgggtct tgcaacagct caagcttgtc tgtggcatag agcttatccc
     6961 cacaagacca ggcaaatcca accaaattat ccgtatggta attctcacca aaaagctcaa
     7021 agtggaagat agactcttca ctcagcatat cttgactgat ttggtcaaca atagtaaaat
     7081 ccaaactctc agacacatca gctgacgaca catttaaagc ctgctttagc tgtttgaagc
     7141 ccatctcatc gtagaatttc ccaagatttt caacatctgg accactatag accaagtcct
     7201 ctaaaccaat cgcaatcggt gccttggtat caatggtcgc tagtgtttta gacaaaaagg
     7261 cctgttcctt gtcattgatg agattttcct tcatcttaga agtcttcatt ccatcaatat
     7321 tttcataaat cccctcaagc gaaccatgct ccagcaagag cttaataccc gtcttttcac
     7381 cgactttggt caccccaggg atattatccg acttatcacc catgagcgcc ttgagatcga
     7441 taaactgagc tggtgtgagg cccatttctt ccatgaggta atctggcgta aaggcctcaa
     7501 actcagccac acctttcttg gaaatttcaa ccaccgtatg ctcatccgtc agctgaatca
     7561 aatccttgtc cccactgaca atggtaatat caaaaccatc ctgctctgct agcttatcca
     7621 gcgtcccaat gatgtcatcc gcctcatact gagccagctc atagtgacga atccccatat
     7681 gatccagcaa ctcacgaatg aaaggaaatt gctcacgaaa ctcatcagga gtcttggccc
     7741 gaccaccctt atagtccgca tacatctctg tccggaaggt cgtctttccc gcatcaaaag
     7801 ccaccaaaat atgactcggc tcaacccgct ccaacaaatg actcaacatc aactgaaaac
     7861 cataaatcgc attggtatgc aaaccagccg cattcttaaa acggtccaac tgctgataca
     7921 gcgcaaaaaa cgcccgaaaa gctacagaag acccatcaat caataataat tttttcttat
     7981 ccatacaccc attataaagg aaagaatcaa aaaataccat tgggaagagc tagagcaagt
     8041 atttttcaaa ctttttccga ataaatagat agagccagag aatttagtaa acctagattt
     8101 aaaaatgtgc tataatatag tatattgaat ctataatagt acaccttgac tgctaaaata
     8161 tttctataaa ttaatttgac tttcctgata gagttattca catcttattt caactcacta
     8221 tagaaggagg aataggagga ttctcagaca tccgggcatc agcccaacta atgatttgat
     8281 tgctaagaaa atattcagca atccagaaat cacttgtcaa tttattcgcg atatgctgga
     8341 cttgccagca aaaaatgtga ccattttgga gggaagcgat attcacgtat tactctccat
     8401 gccttactcg gtgcaggatt tttataccag tatagacgtc ttggcggagt tggataacgg
     8461 tactcaagta attattgaga ttcaagtcca tcatcagaat tttttcatca atcacttgtg
     8521 ggcttacctg tgcagtcagg ttaatcaaaa tcttgaaaaa attcgtcagc gagaaggtga
     8581 tactcactag atctacaaac acatcgctcc tgtttacgtc attgctatcg tggatagtaa
     8641 ttatttctca gatgacctgg cttttcatag ctttagtatg cgcgaagaca caacaggtga
     8701 ggtattggcg attaccaaca atggacagga aaaccatctg gttaagatgg cattcttgga
     8761 attaaaaaat acagagaaac cagcaaagac aaggttcgca agccatggtt ggagtttttc
     8821 ggcaacaagc cctttaccca gcaaccgcaa cgagccatta cccaagcaaa tcaactgctg
     8881 gactacaaga gctggtccga ggaggacagg aaaatgttta gtcaactaca tatgcgagaa
     8941 gaacaagtct tgttagcaca ggactatgcc ttggaaactg ctagggctga aggccttgaa
     9001 caaggactag agcgtgggaa agttgaagga agggcagaaa ggaaactttt tgccttccta
     9061 gacatagtac gccaaggtct tctgacttct gaggttgcca gccagcaatt aggtatgtca
     9121 gtatctgaat ttgaggcact gttgtaaaat ggctccataa tatccatagg ggatttaccc
     9181 actatggata ttatggagcc tattttgtgt aaaaaaaagt cccatatgac ctataatgaa
     9241 aagcgacaaa acaactcatt agaaagaatc atatggaaca attacatttt atcacaaaat
     9301 tactagacat taaagaccct aatgtccaga ttttaaacat catcaataag gatacacaca
     9361 aggaaatcat cgccaaactg gactacgacg ccccatcttg ccctgagtgc ggaaaccaat
     9421 tgaagaaata tgactttcaa aaaccttcta aaattcctta tcttgaaacg actggtatac
     9481 ctactagaat tctccttaga aagcgtcgat tcaagtgcta tcactgttca aaaatgatgg
     9541 tcgctgaaac ttctgatgac gtacagtcat atttcttctc tttttattat atcacagttt
     9601 taaatctagc tttactagat tcaccgctac tatctattta ttcggaaaaa agacgaaaaa
     9661 acctgagaat catctcaggc ttggtcatta aatttttttc tcaatatcga aaagtggaga
     9721 aagtggtcgt ttttcatgaa tacgtacgat agcatcccct aggagatgag cgattgaaat
     9781 ctgctcaatc ttatcaatca aacgctcttc tggcagatag atggtatcca aaacaaccaa
     9841 tttcttaata gctgattttt ggatattgtc cgtagcagga ccagaaagaa ctgggtgcgt
     9901 acagcttgca tagacttcaa cagcaccagc ttccgcaaga gcatctgccg catgacaaat
     9961 cgttccagcg gtatcaatca tatcatcaat caagatacaa gtcttgcctt caaccttacc
    10021 gatgatgttc ataacttcac tagtattcat cttatcaacg ctacgacgtt tatcaataat
    10081 agcgatagat gttttcaaaa attctgccaa cttacgagca cgagtcaccc ctccatggtc
    10141 cgggctgaca accacatagt cagaaccaac cataccacga cgctcaaaat aatctgcaat
    10201 cagaggagca cccatcaaat gatccacagg aatatcaaag aatccttgaa tttgcgcagc
    10261 atgcaagtcg atggtcaata aacgatccac tccagctact tcaagcatat ttgcgacaag
    10321 ttttgaagtg attggctcac gcgctctcgc ctttctatcc tgacgtgcat acccatagta
    10381 aggcatgaca acattaacag attctgcact cgcacgcttc aaagcatcta ccataatcaa
    10441 aatttcaagc agattgtcat ttacaggcga actagttgat tgtaagataa agacgtgttt
    10501 cccacggatt gattcttcaa tgttgacctg aatctctcca tctgaaaatt ggcgaacact
    10561 tgatttcccc aactctatcc caatctcctg cgccacacgt tctgccaatt ctttattaga
    10621 agaaagggca aacagcttta aatcagaaaa agacatgatt tcctccggta tatatgtata
    10681 acttgtgctt ttcacaagat tttccatcta ccattgtagc gctttttgca ctatttttca
    10741 atcaaaaata aaagaagggc accatatttg tacccttgca tcattctttt gaaaaatatt
    10801 ctaggtcatc aactcattgt gtttctcaac aaagcaataa gcatgataaa aaccatagag
    10861 agcaatagcc gtaaccactg gaatcgctaa aggcaactct gtttccaact ccacaaaagg
    10921 agagttaaac aagaagtgag ttcccaaggc taaacctaga aaaataagtc cctgtttctt
    10981 gccaaccttc tgtcctttat aggctctgta aagcaagtaa acacctacta cagctagacc
    11041 tgaaaaagtc cagtgagagg caattcctga gatgatacgc tctaaaattc gcgaaatagt
    11101 aaagtcaaag ccctctggca aatccgtacg aatataacca atatcctcaa tcatttggaa
    11161 tcccaaaccg gaagcaattc caagtaaaaa caaagatttt aattttcgca caggaatcaa
    11221 agccaaaaca aaaacaagtg gcaataattt caagggttct tctaccaaag gagccacaat
    11281 agcactttca aaggcattta aaaatgtact atctgggaaa agaaccccca gtaaatcatg
    11341 gatataagta ttagcaaaac tagacaacca tcctgaaagg aacatccctc ccaataaaga
    11401 cagaatcaaa accttctttg gcaattccca tttttcccaa tacggaagag aaaataaaga
    11461 gccggaatca tgtaaaagag agctagaaag atagaaattc ccattagtcc atattccgca
    11521 cctgacctcg aaccgtccgt atagtagatg gtttcatact gtaaaccaat acatagcaat
    11581 aaaataaaaa taaataaaat actgcttttc ttcatacact ttctttctaa atgaagtatt
    11641 tataattcta cgactgtcat acttcctgta tcaacattgt aaatagcacc agagataatg
    11701 acatcgtctg gtattagggg agactcgata agcagttgca tatcctcgcg tacactctct
    11761 tctatatctt ggaagggcaa gaagtcctgg tctgacacat ctacacctaa ttcctctttt
    11821 aaatactcct gaaaaggttc attttcaaag gtctgagcac cacagtctgt atggtgcaat
    11881 accacaatct ctcttgtccc catttgttgc tgggaaataa ctagcgaacg aatcatgtct
    11941 tcagtcactc gaccacctgc attccgcaag atatgagcat ccccaagtgc caagcccaga
    12001 gcttgcgcaa cgtgcagacg agagtccata caggtcacaa tagctactct tgttttgggt
    12061 ttaagtggca gatttaactg cccatgtagg gcaacataag cctgattggc ttgcataaac
    12121 tgttcaaaat acgacacgat tccctccttg aaaatttgat agtcaaatat ttctcctatc
    12181 ttatcatttt taagagaatt tgtcacggat tatgcaaaga cctttttcaa aacttcctga
    12241 atcgttgtca cgccaatgac ctgaatttcc ttaggcagag tgattcctgt caaggaattc
    12301 ttaggtacat aaatcttagt aaagcccagt ttagcagctt cgttgatgcg ttgctcaata
    12361 cgattcacgc gccgaatctc tcctgtcaag cccagttctc cgacaaaaca ttcctgagga
    12421 ttagttggtt tgtctttgta gctcgaagca atagcaactg caacagccaa gtcaatggca
    12481 ggttcatcca atttaacacc accagcagat ttgagatagg catcctgatt ttgcaagaga
    12541 agccctgccc gtttttccaa aacagccata atcaagctag cacggttaaa atcaagtcct
    12601 gtcgtagtac gcttggcatt tccaaacatg gtcggtgtta ctaaagcctg aacctccgcc
    12661 aaaatcggac gcgtcccttc catggttaca acgatggagg aaccagtcgc cccatccaaa
    12721 cgctcttcta ggaaaacttg actcggattg agtacctcaa ccaagccgcc cgactgcatc
    12781 tcaaaaatcc caatctcatt agtggaacca aaacgatttt tgaccgctct caaaatacga
    12841 aaggtgtggt gacgctcccc ttcaaagtaa agcaccgtat ccaccatatg ctccaacata
    12901 cgaggcccag ccaaggttcc ttctttggtc acatgaccta cgataaagat ggcaatgtta
    12961 ttggtcttgg ccaactgcat gagttcagcg gtcacttcac gtacctgaga aacagacccc
    13021 tgcacccctg aaatctcagg agacatgatg gtctggatgg aatcaataat aagaaagtct
    13081 ggctggatac gctccacttc tgcacgaaca ctctgcatat tggtctctgc atagagataa
    13141 aactcactat caatatcacc taagcgctct gcacgtagtt taatctgctg ggcagactcc
    13201 tccccactga catagagaac tgtccccact tgggacaact gggttgagac ttgttggaga
    13261 agagttgatt tcccaatccc aggatcccca ccgatgagga cgagacttcc tggtaccact
    13321 ccgcctccaa gcacacggtt gaattcctcc atctccgtct tggttcgatt gacattgatg
    13381 gaagtcacct cagctagttt catgggcttg gttttctcac ctgtcaagga cacacgcgca
    13441 ttcttgacct cggcaacctc aacctcttcc acaaaagaag accaagatcc acagttgggg
    13501 caacgtccca gatatttagg ggaattatac ccacaatttt gacatacaaa tgtcgctttt
    13561 ttctttgcga tgacaaacct cttcctatat ctctaactca cactcaatca cttggcaaaa
    13621 atcaatcttc tcatttggca caaactggcg catgagcatt cgatgagcaa caactaccac
    13681 agtctgatgt tctcgatact tagacataca ttctagaaac cgagacttca tttccgtagc
    13741 tgtctcatat tgaataggac tattaggaag caactccccc ttgttttcta aaaacagtct
    13801 tctagctgtt tcaaagtttt ctattcctgt tttatagacc tgccattcat gtaataaagg
    13861 ctctactctt aaaggaagac ccgtagcaca gaccacatac gaagccgttt ctaaagctct
    13921 tgtgactgca gaagatacga ttatttcagc tgacgagagt aaaggatttt tgctcaattt
    13981 ctggacttgc tgccgtccca tctcagacaa gggtgccaaa tctatcccaa atcctatata
    14041 agaacgctcc tctaactcac ggtaatctgg ctccccatga cgtacaaaga taatcttcat
    14101 tctagtgccc tgtcgatcca aatccaccag ttcgaacgcc accagctgca tctccatctg
    14161 caattaagaa agtagcaaaa acagcctgga caatacgttc cccaacttca agaacaacct
    14221 cttggtctgt gatattcttc atctgcgcaa aaatatgccc ttcatttcca ggatttccat
    14281 aataatcccc atcaatgacc ccaactgagt taattaaaac caagcccttc ttacgaggat
    14341 ttgaagaacg atcatagagg tagagaacct cagtcggctg catataagcc ttaacccctg
    14401 tcggaaccaa gacaatctct cctggcgcaa caactgtacg cacagcaacc tttaagtcgt
    14461 aaccagccgc atgcgctgtc tcacgcttgg gcaataaatt ttcatctgta aaacttgaaa
    14521 ccaattcaaa accacgaatt ttcataattt tctcttttct attatcattt attctagatt
    14581 attctatctt atttattcgg aaaaagcacg aaaaaagagc acacaattca aatcgcttag
    14641 ggctgctgga ttcctcccct gacccgcttc acgcagaact gttgctccac tatttattat
    14701 atcacattcc tattcatttt aaaagcaaaa ttattttttc cgtctatttc taaaaaaatc
    14761 ctgcatgata gctgcgcatt catcttccaa aattcctatt tcaacctcca cacgatgatt
    14821 gagacgctca tctgtcaaga tattgtacaa actcccagca gcgccaaatt tctggttttt
    14881 agccccatag accacatttg gaatgcgggc aagcccaatc gctccactac acatgacaca
    14941 aggttcaatg gtcacaaaaa gtgtgcaatc cagcaagcgc cagctctcct cactcaagtt
    15001 cgcatcctct atagccataa tttccgcatg cataaccgct cgctgtaatt cctcacgcgc
    15061 attatgccca cgaccaatga tttccccatc tttgacaatc acacaaccaa ttggaatttc
    15121 atcgtgttca agagcaatct cagcctctct caaagcctcc ctcataaaga cttctttttc
    15181 ttcaagcgta taatacatct cttttctctt ttcctactta tcgattttat tattatatca
    15241 tgaatcccaa cacaaaaaaa gccaccgaat gcggtgactc tatagggaga ttattatgaa
    15301 aaagaaaagt ttaggatatt tgttacaaca agttaggagg tcttcttgta accgtctata
    15361 gtatacccga cctatcttaa acaaatctta aaaatctctt aggaccaaac actttctaaa
    15421 atatttgttt gttcacgacc aggacctact gagaaagtag aaatacgaac gccaaccaat
    15481 tcactcacac gacgaacata gttacgcgca ttctcaggaa gatcttccaa attgcgaact
    15541 ccggtaatat cttctgacca acctggcaac tcttcataga taggcttgca acgtttcaat
    15601 tgctcaagac tagctggata gtagtcaata cgttgaccgt caagatcata ggccacacag
    15661 attttcacag tatccaaacc gctcaaaaca tcaatagagt tcaaagaaag gttagtaata
    15721 ccagaaacac gacggctatg acgcatcaca actgagtcaa accaacctac acgacgtgga
    15781 cgaccagttg ttgtaccata ctcatgaccc acttcacgga tacgttctcc cacttcatca
    15841 aacaactcag ttgggaaagg accatctcct acacgactcg tataagcttt acatacacct
    15901 acaaccttgt caatcttgct tggaccgaca ccagaaccaa ttgtcacacc accagctaca
    15961 gggtttgatg acgtaacaaa tggataagta ccttggtcga tatctagcat aacaccttgt
    16021 gcaccttcaa aaagcacacg tttgccatta tcaagcgcat cattcaagat aacagatgta
    16081 tctatcacgt atttcttgat ttgttgacca tattcgtaat attcttcaaa aatatcatcg
    16141 aaaacaatcg ctttactgtc atacaatttt tcaaaaagac gattcttttc agcaaggtta
    16201 cgttctaaac gctcacggaa aatatcttta tctaaaagat ctgcaatacg aattccaaca
    16261 cgagcagcct tgtccatata agctggacca attcccttaa ttgtcgtacc aatcttattg
    16321 tcgcccttag cttcttcttg caagcgatcc aactcgatat gataaggcaa aataacatgc
    16381 gcacgatcag aaatacgcaa gttatcagtt gttacacctt cctcatgaag atagctcaac
    16441 tcttttacaa gagatttagg atttacaacc ataccattcc caatgacaga tattttttca
    16501 gggaagaaaa tcccagatgg aatcaagtgc aacttaaatt tcttaccgtc aatcacaatc
    16561 gtgtgaccag cattatcacc accttggtaa cgtgcaatca cttctgcatt cgctgaaagg
    16621 aagtctgtaa tcttcccttt accttcatca ccccattggg tacctacaac aacaactgaa
    16681 gtcataatct tgtctgagcc ctcaggctct tcctttctca catacatggc aggactctca
    16741 cctgcaatta tatcttacaa tttattataa taaaaaatcg cctttttatc aagaagaaac
    16801 aatagaaaga tttgctattt ccaactatta aaaaatgatt tagaaaaatt actagc
//
LOCUS       DBERCA010000041        16384 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.041, whole genome shotgun sequence.
ACCESSION   DBERCA010000041 DBERCA010000000
VERSION     DBERCA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.041"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            40..354
                     /locus_tag="ACL719_001478"
     CDS             40..354
                     /locus_tag="ACL719_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HGS0699422.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIATAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            472..2451
                     /locus_tag="ACL719_001479"
     CDS             472..2451
                     /locus_tag="ACL719_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0699423.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFISIFEKIK"
     gene            2461..2775
                     /locus_tag="ACL719_001480"
     CDS             2461..2775
                     /locus_tag="ACL719_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HGS0699424.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2806..3312
                     /locus_tag="ACL719_001481"
     CDS             2806..3312
                     /locus_tag="ACL719_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699425.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            3392..4738
                     /gene="celB"
                     /locus_tag="ACL719_001482"
     CDS             3392..4738
                     /gene="celB"
                     /locus_tag="ACL719_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGS0699426.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            5118..5294
                     /locus_tag="ACL719_001483"
     CDS             5118..5294
                     /locus_tag="ACL719_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699427.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            5704..7917
                     /locus_tag="ACL719_001484"
     CDS             5704..7917
                     /locus_tag="ACL719_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGS0699428.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(8139..8615)
                     /locus_tag="ACL719_001485"
     CDS             complement(8139..8615)
                     /locus_tag="ACL719_001485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGS0699429.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            8838..12038
                     /locus_tag="ACL719_001486"
     CDS             8838..12038
                     /locus_tag="ACL719_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGS0699430.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            12418..12794
                     /gene="tnpA"
                     /locus_tag="ACL719_001487"
                     /pseudo
     CDS             12418..12794
                     /gene="tnpA"
                     /locus_tag="ACL719_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12976..13038
                     /locus_tag="ACL719_001488"
                     /pseudo
     CDS             12976..13038
                     /locus_tag="ACL719_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13182..13811)
                     /locus_tag="ACL719_001489"
     CDS             complement(13182..13811)
                     /locus_tag="ACL719_001489"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HGS0699431.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(13821..14822)
                     /locus_tag="ACL719_001490"
     CDS             complement(13821..14822)
                     /locus_tag="ACL719_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGS0699432.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(14853..15494)
                     /locus_tag="ACL719_001491"
     CDS             complement(14853..15494)
                     /locus_tag="ACL719_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGS0699433.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(15513..16328)
                     /locus_tag="ACL719_001492"
     CDS             complement(15513..16328)
                     /locus_tag="ACL719_001492"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGS0699434.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         5272 a         2661 c         3406 g         5045 t
ORIGIN      
        1 gcacagcttt gattggattt aagaaaagga gaaaatatta tggcaaaagc tttaattatt
       61 tgtgcagcag gaatgtcttc atcacttatg gcacagaaaa caacagagtt tctaaaagga
      121 aaagggaaag atattgtagt tgatgctatt agtgctactg aaggagggaa agctatcgca
      181 acagcagctt ttgatttgta tcttgtcagt cctcaaacaa aaatgtactt taagcaattt
      241 gaagaggctg gttctaaagc aggaaaacca gttgtacaaa tacctcctca agcctatatt
      301 cctattccaa tgggaattga aaaaatggct aatttgattc ttgagaatat ttgatgaaca
      361 catgaaatgt caataaatat ttaagttaag ataaattgtt ttgagtttga tataaattga
      421 tttaagtgta taatatgctt caatatatga aaacatacgt tttaggaggg aatgaatgtg
      481 ttaaacatta aagagaaaga tatccttcaa tttttaataa aaaataagga acgttttgtt
      541 acaagcaaag aactggcaga atatttatcc tgttcagata gaactgtccg taatgtactg
      601 aaattaattg agaagacaat gattattcaa ggagtccggt tgatttcaaa acaaggtcaa
      661 gggtatcaaa tattttttga gaatcaaggc gcttaccagg agtttagaca aacttatgaa
      721 ctggaagaag actatacgaa aactgcggtt tcaaaaggtg atgatcgttt agtatttatc
      781 ttaaataaat tattatttga gcaagttcca gttctttttg atgatttagc tgatgaactt
      841 tacgttagtc gttcaacatt atctcatgat ttcagaaaga tacgagtgat gttatcagag
      901 tataatttgt ccattgagag tagggcgaat aaaggagttt atgtctcagg cgaggaacgt
      961 gataaacgac gttttatcat aaattatttc ctagagaatc aattttttaa aacaatacat
     1021 tgctatgtaa aatttaactt ttttgctcaa actgttccat tagaagaatt tgctagaatt
     1081 gttttggatg aatgccaaga ggccaaccta aaactatctg attttgtact acagaattta
     1141 gtagttcaca tagcattgtc tgtgattcgt ttaaaatccg gatttgaaat aaaaaatata
     1201 gactgtcaga tgactgatga tgcaatagag agaaaggttg ctcaaagaat tctttctaaa
     1261 gtaagagaag taacaaatca ggaatttcca gttcaggaaa tagactacat tactttgcat
     1321 ttgttagcta agagtcaaca atgtcaaaaa aatcagaaga atatttctga agaagtttta
     1381 aaaaaatctt tatttaaaac atttcagaat ttaggattag atgatatgta taacttttct
     1441 tcagatttcc aactaattga aggattgata actcatctaa tgacactaca agtacgttta
     1501 gaaagccgga ttaccttaaa taaccctttg gtagatgaaa ttaaacagaa ttatagtgat
     1561 atattcttta tgactagaga aattcttgca aatatggaca agttcttgga gtggacaata
     1621 tcagatgatg aagtagcata tgtaagtctt cactttttag ctgctatgga acgtagcaaa
     1681 gagagtacta agtttaatat tcttgctatt tgtgcaacag gatttggtgc agcccaaatg
     1741 ttaagaaatc gtttggaaac ggagtttggg aagcgagtag aagttgttga tgtaataggc
     1801 tattatgaat tgaatcaaga aaaacttaaa ggaatagatt ttatagtgtc agctgttgat
     1861 ttatcaaatc tatattttca aattccagtt tttaaagtta gtgtgtttct taagagtgat
     1921 gagatggaaa tgattagaaa agctatggat cagatgcaag tttctagtta tgtacaatca
     1981 tcaaagatta ataaatttga aaacaacagc tttagacagt attttagtaa agaaaatttt
     2041 ttaatatgta ctgaatcaga caaggtaaat ttacttgaaa agatggttga gagtttatct
     2101 gttggtgaat caaatgagtt tgaacaatct ttactttatg gaattaaaca gcgcgaagaa
     2161 ttaagttcag ttgttttttc tgaaaaaatt gcagttccgc atcctattca gccctttggt
     2221 acagagggaa aggtttcggt tgcaatctgt aaagattcgt tactctggga taatcaaagt
     2281 tcatatgtcc agctagtatt tcttctatct ccatcaatat atgggaacga aggtttggca
     2341 actgtgacta aaaagatagt ctctctgaca gagaatgatg agttgcaaaa tcagctaata
     2401 tcttgcaata actttgaaga ttttattagc atatttgaga agataaaata ggaggttcat
     2461 atgaatcaag aggaactgca ggttgcagca tttgaaatta tcctgcattc gggaaatgca
     2521 aggtcagaaa tacatgaagc atttgcaaaa atgagagaag gtagttttga tgatgcagaa
     2581 agtaaattaa atcaatcaaa tgagataatt cttgaagcgc atcatgcaca aaccaaactc
     2641 ttgcaagaat acgcaagtgg agtggaaatt aaaattgaaa ttatcatggt acatgctcaa
     2701 gatcatttaa tgaccactat gacattatta gaagtagcaa aggaaatgtt agctctttat
     2761 aagaaagtta actaaatatg tgttgttata taaggaggaa atgatatgga taaaaaagaa
     2821 cgacaaaaga ttgaacaaca acgtcgagag atggctctaa ctaatacttt ttttaatcga
     2881 tatcttcttc tacgttattc aatagcactc ttcttttttg ggaatattta ttggcttttg
     2941 aatcagttta ttaatccctc gcctattatt attttcccca ttatgctcat agtattttct
     3001 attttagcaa ctgttgaaca atttaaactc tatggaaata ggaaagaaaa gttgggaata
     3061 acactaatgt ttgttagaat acaaatactt atttcaatag gattacttgt tttaacttgg
     3121 acaagttggt ttaagaatct ttttccgatt tttgaaaata accaagtagc acgtttattt
     3181 gtttttgtag ttcttctatt aggtttagtt ctgagtttgt tagacattag aagaattaaa
     3241 aaaatttata aacgaacaga taaggcttat caacaatttg tccaattgga aaagaactca
     3301 cttagtttat aatcctgatc tagtataatt tgtatttgaa actagtcttt agaaagtaaa
     3361 aattattatg ttttattaag gaggtctatt tatggatcaa caaaacgggt tgtttggttt
     3421 tcttgaaaac catgttatgg gaccaatggg caaacttgct cagtttaaag tagtgcgtgc
     3481 tatcacggct gcaggtatgg ctgctgtacc atttactatt gtaggatcaa tgtttttggt
     3541 attcagtatt ttgccacaag ctttctcatt ttggccaatt gtggcagata ttttctctgc
     3601 ttcatttgat aaattcacat cactttacat ggttgcaaac tatgcgacta tgggttctct
     3661 atctctttat ttcgttctat cacttgcata tgaattgaca aaaatttatg cagaggaaga
     3721 agaactcaat atgaatcctc ttaatggtgc cttgctcgcc ttgatggctt ttgtcatgac
     3781 agtaccgcaa atcatttttg atggtggaat gatgaagact gtgacaagtc taaaagaagg
     3841 tgcagtaatt gcagatggat gggcaatggg aaatggagtc gcacgttttg ggacaacagg
     3901 gatttttacc gcaatcatta tggcaattgt gactgttctt atttatcgta tgtgtgttaa
     3961 acataattgg gttattaaaa tgcctgaagc tgttccagaa ggagtttctc gtggatttac
     4021 cgctttggtt ccgggatttg ttgttgcatt tgttgttatc tttatcaacg gtcttcttgt
     4081 agcaatggga acagatattt ttaaagtcat tgcaattcca tttggttttg tatccaatct
     4141 gactaattcg tggattggtt taatgattat ttatctattg actcaactac tttggattgt
     4201 aggtatccac ggtgcgaaca ttgtttttgc atttgttagt ccaattgctc ttgctaacat
     4261 ggctgaaaat gctgctggcg ggcacttcgc tgttgcaggt gaattttcta atatgtttgt
     4321 aattgcaggt ggttctggtg caactttagg actatgttta tatattgctt ttgcctctaa
     4381 atctgaacag cttaaagcaa taggacgagc atctgtagtt ccagccttat ttaatattaa
     4441 tgaaccatta atttttggat tacctattat ctataatcca gccttggcta taccatttat
     4501 tttagcacca atggttactg ctactattta ttacgtagcg aattctctaa actttattaa
     4561 gccaattatc gcacaggttc catggccaac tccagtaggg attggagctt tcttagggac
     4621 agcagatctt cgagctgtat tagttgctct agtatgtgca tttgcagcat tcttagtcta
     4681 tcttccattc atccgtgtat atgatcaaaa attggtgaaa gaagagcaag gtatctaata
     4741 agtaaaaaag tatactcgat gatagaatcc atatagttta aataatgagt gttaatatta
     4801 gcagtgaaga ggtcttttta agatattctt ctctgctttt tcatattttt tcgctatgta
     4861 gggtatgaag caaaatagaa agtagttcga atagttgata aacaggaaac tgctgttatt
     4921 taaaaaaaac aataaagtaa agtatgttat cgtagaatac gatacaatag taaaaaagca
     4981 aaaagaatgc gttttatatt tgggtttgag gtttataaaa taggataagc gtgctgaaaa
     5041 cttagatatg acaaacaaaa taaaattttt aaaagctaca tagaaacatg gtataatgta
     5101 tctaaaggag gtagcatatg tccttagata tagataaaga aaagatgaca atcatgggaa
     5161 tagcttttga aaacagatcc gtttttaaga gtgtttggta tgctttaagt acaaatatga
     5221 ttgaaggatg gcgacctacg gttagtgatg ttgaaaaatt acgagatgaa gctcttgctc
     5281 tggggatgac ttaatgaagc gcaactgtta tgagtcttat gactatgttg atccggataa
     5341 tttatatacc gatccgggtt cttcagttct aagaaataag caggaaggaa aaaagcacgc
     5401 gagcttgaat atcgtatgat tgctagtaag agtttgaaat tatttatcaa tcctatctta
     5461 gttaatagtt taaaagtagt ttgtttcaca actgcataag cttagaaaag ttgaaaagtg
     5521 ataactcttt aataaactct tggtttgtta aaagatattt tgtccactac attttttcaa
     5581 ttgactatta tttgtcatag atataacaga tagaacagat agaacattgc attcagaata
     5641 atgaaaaacg catcaaaaat ggtgcgttct gtttttatgc taccataaaa ggaggaaaac
     5701 aaattgaaaa tttttaaggg agagttttat cgaatctctg tattaacaga caagctagta
     5761 aggttagaat actctcaaac tggaagtttt gaggatagaa cgacacaact tatctataat
     5821 agagattttg gccaagtttc gttagattat atcgagacat caaacgtact agatattatg
     5881 acggactatt ttcatctgca ctttaataaa ggagaattta acgccgaaaa tttatttata
     5941 gaattaaaag gaaattttgc cgtatatggt agtcgctggt attttggtga atctattgaa
     6001 acgttaaaag gaacagctcg gactctggat aaggcagatg gagcaatctc gttagaagat
     6061 ggaattatta gccgaaatgg tatagcctta ttggatgatt ctcaaggatt tatttgggat
     6121 gaacaatctg gttatattga gagagaaaat caaattgacc tgtatttctt tgcctatggg
     6181 catgattata gaggagcaat cagagacttt taccatttga ctggttcaac acccttgttg
     6241 ccaagatatg ctttaggcaa ttggtggagt agatattggc cttatacgtc ggatgaatac
     6301 ttggatttaa tagacagatt tgaaacagag aaaattccat tatctatcgg tgtgttagat
     6361 atggattggc atataactga cattccagct cgttttggaa gtggctggac aggatatagt
     6421 tggaatagaa acttaatacc aaatccagaa cagttattgc aacaacttca tgatagaaag
     6481 ttaaaactct ccttaaatgt ccatcctgct gatgggatac gggcttatga agaagcttat
     6541 cctcaaatcg caaaacggtt ggggttaaat gtagaactag aagaacctgc tatttttgat
     6601 ttttttaatc cctcttttag ggaagcctac tttaaagatg ttcattatga gctagaaaag
     6661 cagggagtag atttttggtg gattgactgg caacaaggga cacaaggtat gctagatcca
     6721 ctttggcttt taaaccatta tcactatcag gatagttgta aaaatgcaga aggtggtttg
     6781 attttatcaa gatatgcagg tcctggtagt caccgctacc ctgttggttt ttcaggggat
     6841 actattatta gttggaattc cttaaggttt caaccctatt ttacagcgac agcatctaat
     6901 atcggttata gttggtggag tcatgatatc ggtggacata tgttggggga ttatgacgaa
     6961 gagctacaaa ctagatggct acagtttggt gtttttagtc cgattactcg attacatagt
     7021 tctagaagtc cttttaatag taaagaacct tggttctttt cagaaacaac atctaagatt
     7081 atgaagaaat accttcgttt gagacatcag atgattccct atctatatac catgaatgta
     7141 aagacacatg aggaaggtgc cccattaatc agtccaatct attatttcta tccagagaat
     7201 gatgagagct ataatgttcc aaaccaatac ttttttggaa cagaactgat ggtggctccc
     7261 attgtagaaa agatggattt gacattccaa tctgcaaaag tagatgtatg gttccctgaa
     7321 ggtgaatggt atgacttctt ttcagagaaa aaatacacag gtggtgtgaa gttaagtgtt
     7381 tatagggaca tctcgactac gcctgtgttt gcaaaaagtg gtgcaatcat tcccttggtt
     7441 ggttctgaga taggtatggg tgttgattta cctgaggttg tagattggta tgtattccca
     7501 gggaaacaac atttttttga aatgcttgaa gatcaaaatg gtcaaagata taaaacaaga
     7561 ttatcaatcg attgggaaat gggaatggta gagttagcat tacaaggaga ttctagtatc
     7621 gttccaagca atagaaaaca tagaattcat tttaaaggaa cgaatgtgtc tataattgaa
     7681 ttgccaaata agaatgatac agctaaattt gaatggaaag ataataaaag gacatcttta
     7741 aatgacgaag tttttagact actaaagaca gcttctcttc catatgaatt aaaagataga
     7801 ttgttaaatc aattcatcaa tgcgaaaaac tctcatgact taatgaatat cttgcatcat
     7861 caggataagg aattgagagg gcgtttgttg gaaatgatat ttactagcca aaactaattg
     7921 aaataattag aaagtcattt gtttagtgta tatgaagatt ctgtatggta ttcgttatca
     7981 ctggtcacat tgaaaatgtg aaataagcac ctacaacata gactttacaa cacttcatgt
     8041 ttcatatcat ggggtgtttt tctatttaag tcaaagccca tcaaaaaatc tcactattca
     8101 tcaggttaaa ggaaacctaa tcatagtgag attgtgaatt atagtagagt ttgtatcgcc
     8161 tcttcaattt gttttgggtc tgtttttgaa gaaaagcgtt caaagacttg cccatctcga
     8221 ccgatgagaa acttagcgaa attccattcg attcgttttc ctagtgggcc ggatttctgg
     8281 tcttttaacc agacatagag agggtctgct tccttaccgt tgaccttaat cttggcaaaa
     8341 cgtgggaagg tggtttgaaa atgtaggcta cagaaggcgt tgatttcctc tgcgctgccg
     8401 ggtgcttgtc ccataaactg attgcaaggg aaatccaata tttcgaagcc ctgatcttga
     8461 tagcgttcat agagttcttg aagtccctgg tactggggcg ttaaaccaca tccagtagca
     8521 gtgttgacaa tcaagagaac tttgccacga tagctatcca agggagttgc ttggttgttt
     8581 tggttcaaaa cggaaaaatc ataaagtgaa gtcatgaggg cctcttttct ttttctacat
     8641 tgtaacagtt tttacttttt tcgtcgagtc ggatggaaga tattgttgaa gaataagcct
     8701 tcaatatgat agaaaccgtt tacaaataac tttaaaaatg gtatgattta attaaaccgg
     8761 tttacttagt tgctataaat ctctaatttg agatttagtt cctattgatt ttacaatatg
     8821 tttattggag tgtatacatg caaacaaaaa caaagaagct cattgtgagt ttgtcttcac
     8881 ttgttttatc aggattttta ttaaaccatt atatgacagt tggagcggaa gaaacgacta
     8941 cgaataccat tcagcaaagc cagaaggaag ttcagtatca gcaaagggat acaaaaaatt
     9001 tagttgaaaa tggtgatttt ggtcagacgg aggacggaag cagtccgtgg acaggaagca
     9061 aagctcaggg gtggtcagct tgggtagacc agaagaatag tgcagatgcc tcaactcgag
     9121 tcattgaggc taaggatggg gctatcacta tctcaagcca tgagaaatta agggcagcgg
     9181 ttcaccgtat ggttcctatt gaagctaaga aaaagtataa actgcgtttc aagattaaaa
     9241 cagatcataa agtcgggatt gccaaagttc gtatcattga ggaaagtgat aaggacaagc
     9301 gattgtggaa ttctgcaacg acgtcaggaa caaaggactg gcagaccatt gaagcagact
     9361 atagcccgac tttagatgtt gataaaatca agctggagtt attctatgaa acaggaactg
     9421 ggactgtttc ctttaaggat attgagctgg tagaggtagc agaccagcct tctgaggact
     9481 atcaaacaga taaacagctt gaggaaaaga ttgatttacc aattggaaaa aaacatgttt
     9541 tttctcttgc ggactatact tataaggtag aaaatcctga cgttgcttca gtcaaaaatg
     9601 gaattttaga acctcttaag gaagggacaa ccaatgtcat tgtcagtaaa gatggcaagg
     9661 aagtgaaaaa gattcctttg aagattctag cctctgttaa ggatgcatac acagaccgtt
     9721 tggatgactg gaatggcatc atcgctggga atcaatacta tgattctaaa aatgaacaga
     9781 tggccaaatt aaaccaggaa ttggaaggaa aggtagctga tagcctatct agtatttcaa
     9841 gtcaggcgga ccgcacctat ttgtgggaaa aattttcaaa ttataaaatg tctgcaaatc
     9901 tgactgccac ttatcggaaa ttggaggaga tggccaagca agtgaccaat ccttcttctc
     9961 gttattatca agatgaaact gtcgttcgaa cagtcaggga ttccatggaa tggatgcata
    10021 aacatgtcta caatagtgaa aagagcattg ttgggaactg gtgggattat gaaatcggta
    10081 cacctcgtgc catcaacaat accttgtctc tgatgaaaga atacttctct gatgaggaaa
    10141 ttaaaaaata tacagatgtg attgaaaaat ttgtaccaga tcccgaacat ttccgaaaga
    10201 cgactgataa cccatttaag gctctaggtg gaaacttagt tgatatggga agagtaaaag
    10261 taatagctgg tttactgcgt aaggatgatc aagaaatttc ttctaccatt cgctcgattg
    10321 agcaagtgtt caagttggta gaccaaggtg aaggttttta tcaagatgga tcctatatcg
    10381 accataccaa tgttgcctat acgggtgctt atgggaatgt tttgattgat ggcctgtctc
    10441 aactgttgcc agtcattcaa aagaccaaga atccaatcga taaagataaa atgcaaacca
    10501 tgtaccactg gattgataaa tcgtttgctc ctttgctggt gaatggagag ctgatggata
    10561 tgagtcgtgg acgctcgatc agtcgtgcaa atagcgaggg gcacgtggcc gcagtagaag
    10621 tactaagagg gattcaccga atagcggata tgtctgaagg agaaaccaaa caacgtttgc
    10681 agagtcttgt gaagaccatt gttcaatcgg atagttatta tgatgtcttt aagaatttga
    10741 agacttataa ggatatcagt ttgatgcaat ccttgttaag tgatgcagga gtcgcaagtg
    10801 ttccaagaac aagttaccta tctgccttta acaagatgga taaaacagcc atgtacaatg
    10861 cagagaaagg gtttggattt ggcttgtcac tcttttccag tcgtaccttg aattacgaac
    10921 acatgaacaa ggaaaataaa cgtggttggt atacgagtga tgggatgttc tatctttaca
    10981 atggcgattt gagtcactat agcgatggct actggccaac agttaatcca tataagatgc
    11041 ctggtacaac agagacggat gctaagagag cggatagcga tacaggtaaa gttttaccgt
    11101 ctgctttcgt tggaacgagc aaactagatg atgccaatgc gacagcaacc atggatttca
    11161 ccaactggaa tcaaacattg actgctcata agagctggtt tatgctaaag gataagatcg
    11221 cctttttagg aagcaatatc caaaacactt caacagatac tgctgcaact acaattgacc
    11281 agagaaaact ggaatcaagt aatccatata aagtctatgt caatgataaa gaagcctccc
    11341 ttacagaaca agaaaaggat tatcctgaaa cccaaagtgt ctttttagaa tcatccgatt
    11401 cgaaaaagaa tattggttac tttttcttta agaagagttc aatcagtatg agtaaggctt
    11461 tgcaaaaggg agcctggaag gatatcaatg aaggacagtc agacaaggaa gttgaaaatg
    11521 aatttcttac gattagtcag gctcataagc aaaatggaga ttcttatggc tatatgctca
    11581 tccctaacgt gggtcgtgcc accttcaatc aaatgataaa agagttagaa agtagcctca
    11641 tcgaaaataa cgaaaccctt cagtctgttt atgatgccaa acaaggagtt tggggcattg
    11701 tgaaatatga tgattctgtc tctactattt ccaaccaatt ccaagttttg aaacgtggag
    11761 tctataccat tcgaaaagaa ggggatgaat ataagattgc ctactataat cctgaaaccc
    11821 aggaatcagc tccagatcag gaagtcttta aaaagctaga gcaagcagct cagccacaag
    11881 tacagaattc aaaagaaaag gaaaaatctg aagaggaaaa gaaccattcg gatcaaaaga
    11941 atctccctca gacaggagaa ggtcagtcaa tcttggcaag tctagggttc ttgctacttg
    12001 gggcgtttta tttattccgt agaggaaaga acaactaatt tgttcatcat ctagatgaat
    12061 tacatgaaac tgtggaaaat agaaaaaatc cgaagaaggc caaatatctt cggatttttc
    12121 ttttaaaaaa ggataagttg atacttatcc tttttacttc attatgtgac tatagcgttt
    12181 tgaaactaga ataggacatc ataactgcta aaagatttct ataaattcat ttgattttcc
    12241 taatcaattt attcgtatcc tatttcactc tactatatta gtccgtctcg cacctcacgg
    12301 agcgagacaa aacaaccacc cgctatgcgg gtgcgcgtcg aaggttatac caaaaaaact
    12361 ccaaacgcga tacaataaag gtgttcaatc cacttgtaaa gcgaaaggag aaaaaatatg
    12421 gcacaaaagg ctcatagttt atctcacaca aagtggaggt gttctatcac attgtgttca
    12481 cccctaagta tagacgaaaa gtcatctata atcaatatag aagtagttta ggcgaaatat
    12541 ttcatcgctt gtgtagttat aaaggtgttg aaatgatcga gggtcactta atgccagacc
    12601 atgtacacat gttagtcagt attccactaa ggctaagtgt ttcgagtttc atgggttatt
    12661 taaaaggaaa aagtgcactc atgatgtttg acaaacacgc caatctcaag tacaagtttg
    12721 ggaatcggca tttctgggtg gaaggttatt atgtaagtcc agtagggctt aatgaagcca
    12781 caattaagaa atatagtcaa gattaaagaa attatccagt ggatgatttc ttcacgagta
    12841 tgaaaatttg agaactagta aagtatgata tatagtaaaa tgaaataaga acaggacaaa
    12901 atcgatcagg acagtcaaat cgatttttaa caatgtttta gaagtagagg tatactattc
    12961 cagtttcaat ctactatagc actagataaa ttgagtgtaa aagaatatga ggatcccttt
    13021 agggatagtg gtaagtaata ccaaagtctc ttaaagaggc aagtgacgag tcaagagcaa
    13081 taaggcttga acaacgtaaa agccagcgtc tttaggcgct ggctgatgat ttgggcttat
    13141 agctctggga taaaccaccc gttagacggg tagttatgat tttatctgag tgtaatatac
    13201 tgttgggcaa tctcgctgat gcggtcaaag ttgccttggg aagcgagttt attgagttcg
    13261 ccaccaattc caacggcatc tgcaccagca gcgaaccatt gagggatgtt gtttaggccg
    13321 actcctccgg ttaccattac ggaaacttgt gggatcggtg ccttgactgc agagatatat
    13381 gctggactga gagtactacc tgggaagagt ttgatgattt cactaccggc ttcaagtgca
    13441 gtcgtgatct ctgtgagggt aatacagcct ggaatgtacg gtgtgctgta gagattgcac
    13501 attttcgcag tttcagcatg gaaagatgga gaaacaacgt aatttgctcc agctagaatg
    13561 gcatctctag cagttacggc atcaagcaca gtacctgcac cgatacaaac actctgatcg
    13621 tcctgataca agtctacaag ttccttgatg atttgtcctg catactgatt ggtataggcg
    13681 atttcaatag ctttgatacc gcccttgata caagcaatcg aggcttgtag tccttcttcc
    13741 tttgtatttc ctcgaatgac agcgacaatt ttctgttttt ttagttcaat aatcgtatct
    13801 gatttggtca tgtaattctc ctaacgaatg atatcttgtg catttgccag taaattttca
    13861 atactagttg cggaagtgga gagatggtct cctggaagag tgcatttgag agttgcgctc
    13921 gcaactgcaa agtcaatggt agtttttagg gaggaatgat ggagtagttg gtaaagggca
    13981 ccagatataa aggcatcccc gctaccaatt cgttgataga ctgcagtttt tagttggaca
    14041 gactcttcaa atcgttcttc cagagcatag gcttgataga catttttgtc ttgctcatca
    14101 ctagatcgga gggtatggaa aatggccttg aaaccatagg cttcttttaa aagtcgcatg
    14161 cgattttcca cctcttctag gctagcactg tctcttggaa acatctctag attttggtca
    14221 tcaatcatga gaggatcaat accgaagcaa tagtcagtaa aacgtgcaaa cttagaaaat
    14281 tcatacttgg cttctaggac tgaaatcatc tttgttctca gattgagatc cattgaaacg
    14341 acaattcctc ggcgcttggc ttcttccaag agtaggagaa ggatcgcacg gacctcttga
    14401 ccgatagcta tggtgattcc actaaaatga aaatggctaa tcccctgaaa gagagaatcc
    14461 atatctagca tgtttggccg aatctggctg atactcgtat gcttacgatc gtagaaaact
    14521 tcactttgac gacaaccaaa gccgttctcc aaatagtaga ggccgattcg atcgccaagc
    14581 cgacagattg aactggtatc gatttggtgc tgtttcaaga atgtgagaaa acgatctcca
    14641 atctcgttgg cagggagtgc ggtaaagact ttcgttgaga tacccagggc ttgcaaatta
    14701 caagcgatgt taatttctga tccgccaaaa taagtcgaac tggcaacatg atcgccgata
    14761 ctggtggcat ctaatggtga gattcgaatt aatggttcgc caaacagtaa tattttcgtc
    14821 atccattagt tcctttctgg tttagaatag ctttacttgg ctaatacttc tttcaaataa
    14881 gcagcgactt catcatcttg gcagttttca aagaagtatt cttggaagta tttgccagag
    14941 atggtgtctt tgaggaagtc ttggtcgatt gttttaagga tggtcatcaa atcattgtgg
    15001 gtgatttgtt tcacctcgtt taagatacga gcgttgcgtt gttcagggat tacacgttct
    15061 cttgggtagc caccgcccat ttcttcagca aataagcgtt caaacatcaa tttgagggtc
    15121 agttctgccc cccagccaaa tcctttggca taagggatag acaaggcgtt accaccattg
    15181 atttgagaat aaaggtaagc gtcagttggg tccactgcta gaccacagac aacaccaggg
    15241 aagctgttta aagcaagcat agcccctaca cccgtaccac agccggtaac aacaaagtca
    15301 actgcctttg tatttaaaag gatggcagcc attagtccgt tctgcacata agttaattga
    15361 ctttctcctt cttctccacg cataccatag ttaaataatt ggtagtcttt tttatctgtc
    15421 gcttctttta gactatcgta aataatgtga ttcttgctag cttgactatt ttcattgatt
    15481 aatgcgattt tcataagatc ttctttctat ttttactcag gttgttttcc gatgtaggct
    15541 aagataccgc catctacata taggatgtgg ccattgacaa aattgctggc atcactagcg
    15601 agaaagacag cagggcccat caaatcttca ggatttcccc aacgtgcagc aggtgttttt
    15661 gcaatgatga actggtcaaa tgggtggcga gaaccatctt cttgcaactc acgaagaggt
    15721 gctgtttgag gagtggcaat ataacccggt ccaattccgt tacattggat attggctcca
    15781 ccgtattcag acgcaatgtt gcgggtcaac attttcaagc cccctttagc agcagcataa
    15841 gcgctaactg tttcacgtcc cagttcgctc atcatcgaac aaatattgat aatctttcca
    15901 tgccctttct ttatcataga aggaataact gcctttgaaa cgataaatgg tgcgtttaag
    15961 tcaatatcga tgaccttacg gaaatcagcg gcgctcattt cgcacattgg aactcggcgg
    16021 ataataccag cgttattaac gaggatgtca atgacaccaa cctcttgttc gatttgcttg
    16081 accatggctt ggataccgtc ctcgtctgtc acgtcacaga catatccatg ggcttcgatg
    16141 ccaacttcac gataagcttc aatcccttta ttgaccagat cttgattgat atcgttaaag
    16201 acaatagtag cgccggcttc agcgtaggat ttggcaatag caaatccaat tccataagaa
    16261 gcgccggtga tgagagcaat ttttcctttt aaagaaaact gctcaattga gaatgatgta
    16321 tttgtcattt gatacttcct ttcaaatttg ttaagacttc tctataacca aattatattc
    16381 tagc
//
LOCUS       DBERCA010000042        15035 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.042, whole genome shotgun sequence.
ACCESSION   DBERCA010000042 DBERCA010000000
VERSION     DBERCA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.042"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(37..252)
                     /locus_tag="ACL719_001493"
     CDS             complement(37..252)
                     /locus_tag="ACL719_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699435.1"
                     /translation="MCYYVKKYFSKYKWTDLFWILFVILTCLYIGNHDLFTLNHQEFS
                     FRGSVWGLVLALYHLLFIDKFVISNRK"
     gene            319..891
                     /locus_tag="ACL719_001494"
     CDS             319..891
                     /locus_tag="ACL719_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699436.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGIYLAQLWDKNDAIPNNGRINF"
     gene            complement(1936..4194)
                     /gene="glgP"
                     /locus_tag="ACL719_001495"
     CDS             complement(1936..4194)
                     /gene="glgP"
                     /locus_tag="ACL719_001495"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HGS0699437.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGENSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(4220..5752)
                     /gene="malQ"
                     /locus_tag="ACL719_001496"
                     /pseudo
     CDS             complement(4220..5752)
                     /gene="malQ"
                     /locus_tag="ACL719_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            6287..7558
                     /locus_tag="ACL719_001497"
     CDS             6287..7558
                     /locus_tag="ACL719_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0699438.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            7669..8961
                     /locus_tag="ACL719_001498"
     CDS             7669..8961
                     /locus_tag="ACL719_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGS0699439.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            8963..9805
                     /locus_tag="ACL719_001499"
     CDS             8963..9805
                     /locus_tag="ACL719_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGS0699440.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            10058..10858
                     /locus_tag="ACL719_001500"
     CDS             10058..10858
                     /locus_tag="ACL719_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGS0699441.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            10868..11854
                     /locus_tag="ACL719_001501"
     CDS             10868..11854
                     /locus_tag="ACL719_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0699442.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(12190..13131)
                     /locus_tag="ACL719_001502"
     CDS             complement(12190..13131)
                     /locus_tag="ACL719_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS0699443.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(13109..14872)
                     /gene="aspS"
                     /locus_tag="ACL719_001503"
     CDS             complement(13109..14872)
                     /gene="aspS"
                     /locus_tag="ACL719_001503"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGS0699444.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT         4506 a         3216 c         2835 g         4478 t
ORIGIN      
        1 atattaaaag tagccagtca atcatagttc taatctttat tttcgattcg aaattacaaa
       61 cttatcaatg aacaataagt gatataaggc cagtaccaga ccccaaacgc taccacgaaa
      121 agagaattct tgatgattga gagtaaacaa atcatggtta ccaatataga ggcaggtcaa
      181 aataacaaat aaaatccaaa ataaatccgt ccatttatat tttgaaaaat attttttaac
      241 ataatagcat atttttttga ctataataac agtatatgat aaaatgtaag cagatacaaa
      301 taaaaaggag aaaagctcat gaacaaatta atgaaattta tttcggtttt tttgacgtca
      361 attgtgttaa ttgtatcagc gattccaagt gtttcagctg tatacgcttc tgaacaagta
      421 tcacaaattg aaacaaatat ggaacttcaa cctgtcactt ctctaacaga agaacaaatc
      481 aatacacttg caaacgaaat ccaatctttt catccagacg tctcacaaca atggatcaaa
      541 gaagtaatta accgacaatt acaaggcgat tatacaatcc cacctacata ctctccattt
      601 agagcagctt ggcaaggtat tacagttaat caaatgggtg ctctattaga tactgcaata
      661 gctttagcat taggaggaac tactgcaggc cttgcaaatc taattaaagt aaaaggaaaa
      721 catgcagcaa aaagtgctat tcgttcagca atttctagat atctaggtag ttggtttgta
      781 aatgatgttg ctttagaatt cgctatgaat ttattatcac cggggattta tttagcacaa
      841 ttatgggata aaaatgatgc cattcctaac aacggaagga ttaactttta atcatgagat
      901 atacaataaa attaattggg tataacgtat tttcgttgtt tatattcttt atcttatcaa
      961 tgttgtttaa agttcccttt aggtggtgga tcagcattgc aatgacgact tcgtcgtaca
     1021 ttgtatataa ttacttaaag aaaaagagag aaaaggaatg atcttatacc agatcattcc
     1081 ttttctgctt tctgtaaatc cctcggagtg tgtttggcgc tttgatacga agtgtcaaag
     1141 ctgtataaac gttactattt ccaaaaaagt aacagaaaat tcctgctttg taggactgct
     1201 ttgggtgagt aatagacatg tagactcacc ctatctcctg atggcgaaaa gaatttagca
     1261 atgatgcaag ttgttctgaa ctcgagaaaa aacatgccga aatcgaagaa aagcgtgcag
     1321 aacttgaagc gaagaagcaa gaaatgttga agctcttgga taaatagaat ttagaaaaca
     1381 gaaaaaacta aaaaaaatgt agggtttatt tccctacatt cttaaaataa cttagtacat
     1441 aaatgacaat gctgccactg ccgtcaatat tgcaaaaact attttccaaa tttttttatt
     1501 ttttccaaaa atcttatcag gtaacaagct tattacaaga aaggtaataa acaacaccaa
     1561 tttaagtgtg tatatcataa aaacaccttc tttcctacaa ttatattata atactttatg
     1621 taagcgaata taatagatgt gaggtaacca actacgaaaa aatataaggc tgaagacagt
     1681 aaaaaccagc atttaaagcc ttcactgtat ctaggatttc cctattttta gacaagattg
     1741 acccaaatcc acagcattta aaaaagcgaa tttcaattga aactcgcttt tttacaagat
     1801 gtattagtat aaatttgtat cttatactca atgaaaatca aagagcaaac taggaagcta
     1861 gccgcaggtt gctcaaagca ccgctttgag gttgcagata aagctgacgt ggtttgaaga
     1921 gattttcgaa gagtattagt tcaagtgcca gatgtcttcg ttatactgag cgattgtacg
     1981 gtcagatgag aagaatcctg ctttagaaat gttaacgatg actttatcca accatgcgtc
     2041 acggtcttcg tagtcagcaa gcatttgctc tttgactttg atgtagtctt ccaaatcaag
     2101 aagagtcatg aaccagtctt tgttgatcaa ttcattgtaa agacgttcca agcgctcttt
     2161 gtttccagct gcaagaactg catcactaac gatgaagtca accaatggtt tgatagcttc
     2221 acgagcgtag aattcgcttg atttgtaagc tgcttttgcg taaaggtcga taacagtttc
     2281 tgaattttca ccgaagatgt agatgttttc ttctccaacc aactcagcga tttccacgtt
     2341 agcaccgtcc atagtaccaa gtgtcaaagc tccgttcaac atgaatttca tgttaccagt
     2401 acctgaagct tctttagaag caagtgagat ttgttctgag atatcacatg ctgggataag
     2461 gaaacttgct gcagtaacgt tgtagttttc aaccataact acttgcaagt gtggagctac
     2521 tgctggatcg ttagcaataa cttctgacat gcaaaggatt aaatggataa tgtcttgagc
     2581 gattgtgtag gctggagctg ctttaccacc aaagaagatt gtgattggac gagcagggat
     2641 gttaccagct ttgatgtcaa ggtatttgtg gatcacgtac aaagcgttca tttgttggcg
     2701 tttgtactcg tgaagacgtt tgatttggat atcaaagata gaatttggat tgatttccac
     2761 accttggtgt tctttcaagt gacgagccaa tttacgtttg ttgtgagcct tgatgctttc
     2821 caatttttct ttgacagctg ctttgtcttc ataagacaac agtttttcaa gctcatctgc
     2881 ttcatggtgc caaccatctc caagaatctc atccaagtag tgagacaatc ttgggttagc
     2941 atgcataagc caacgacgga aagtgatacc gtttgttttg ttgttgaact tttctgggta
     3001 aaggtcgtag aaggctttca actcagaatt tttcaagatt tcagtatgga gtgctgcaac
     3061 cccgttaaca ctgtatccgt agtggatatc catgtgagcc atgtgaacac gtccgctctc
     3121 atcgatgatt tgaacagctg gatctttgta ctctgccttc acacgacggt ccaattcttc
     3181 gatgattggt accaagtgag gaaccacttc ttgcaagaat tcaagaggcc atttttcaag
     3241 cgcttcagca aggattgtgt ggttagtgta ggcagtcatg ctacgaacaa ttgagattgc
     3301 ttcgtcaaga tcgataccac gtgcagtcaa aagacgaatc aattcaggaa tcaccattga
     3361 tgggtgagta tcgttgattt ggacaactgc gtagtcagca aggtcatgca agttgcttcc
     3421 tttttcgatt gcttcgtcga tgatcaattg cgcaccgttt gaaaccatga agtattgttg
     3481 gaagatacgg agcaattcac cttgacggtc actatcatct gggtaaagga agagagttaa
     3541 gttgcgagcg atatctgtct tgtcaaagtt gataccatct ttaataatag aagaatcaac
     3601 tgaatccaag tcaaacaaac gcaagcggtt tttagtcgct gtttcataac cagtaacatc
     3661 aatatcgtaa agagttgatg tcaaagtaaa gtctgcaaat ggtacttggt agctacggct
     3721 tgagcgaacc aaccagtttt gctctgtcaa ccatgcattt ggaattgttt cttgttggtt
     3781 gtttttaaga acttgttgga aaagaccaaa gtggtagtta agaccaacac cgtcaccatt
     3841 caaaccaaga gtagcaattg agtcgataaa gcaggcagcc aaacgtccca aaccaccatt
     3901 accaagagat ggttccaatt caacttcttc aacttcgatc aagtctttac ctgcagctgc
     3961 aagttctttt ttaacatcgt cgtaaagacc aaggttaatc aagttgtttg acaagagttt
     4021 accaatcaag aactcagctg agatgtagta aacttttttc ttaccagtgt tgactggttt
     4081 ttggctgctt gcaagcttgc tgtagttaag aagagcaagg taaagctctt cattgctaca
     4141 ttctgcaatg gttttattgt aacgattttg tacaaattct tgtagtgata acatgtttag
     4201 ttgtctcctg attattgtct tatttcttta aatctaccaa attttcatta attcggcgat
     4261 aaattgttgt caagtcaagc aaaccttcct cgacagctgg tgtcaattga tcttcagtca
     4321 tacgccaaga ccagtttcca ccaagggtag atgggaagtt catacgagct gcctcatcca
     4381 attctagtaa atcttgcata gttgcaattg ccataaagct aactgatgaa aatactgtac
     4441 gaagcatagc gtgtaccact gtttcgtatt ctttacggtt cgtgtaacga gccatgtact
     4501 cacgagtcgc atcatcaatc tcattacggt accaaccaag aaccgtattg ttatcgtgtg
     4561 ttcctgtgta cataactgag ttagcaggtg ccaagtgtgg gctatcaatg ctttcgtctt
     4621 ctgggttgaa ggcaaattga agaatcttca ttcctgggaa gccagtacgt tcacgcaatt
     4681 cgatcacttc atctgtcatg aagccaaggt cttctgcgat gatgtttagc tcaccaagtt
     4741 cttccttaac ggctgcaaaa agcttgtagc ctggaccttt cacccactca ccaggtgctg
     4801 ctgtatcgga accagcaggg atttcccagt aagattcgaa gccacggaag tggtcgatac
     4861 gaacgatatc gtagattttg aagctttcac gcaagcgttc aatccaccat ttgtagccgt
     4921 ctttgtccat tgcttcccag tcatagattg ggttacccca aagctgacca gttacagaaa
     4981 actcatctgg tgggcatcct gcgatacaag tagccttacc attgacatct gttttgaaga
     5041 gatgtggatt tgcccacata tcacttgaat cttccgctac gtagattggc atgtccccaa
     5101 cgatttcgat gtggttgtcg ttagcgtaag ctttcaattt caaccattgt tggaagaaga
     5161 agtattgagt cacacggtgg taaaccaact tgtctgccaa ttgctcacga tagctttcaa
     5221 gtgctgaagc tttacgagca cgagcatctg catctggcca ttcagtccaa gcaagattgt
     5281 caaaatactc tttgatagcc atatactcag caaagagctc aagccatgat tggttgtctt
     5341 gagcaaattt ctcaaaatct ttaacatctc cgacttcaaa gaaacgtttc accgcttttt
     5401 ctaaaagagg acgacgtgca tagtagattt tagcatagtc aacttcagac gcatcgctac
     5461 caaagtcaac tccttcaagg tcacttgctt ccaacaaacc ttgctccacc aagatatcta
     5521 aatcgataaa atgagtgttt cctgcgaagg ctgagaaaga ttggtaagga gaatccccgt
     5581 aactagttgc tcctaatgga aggatttgcc agtaacgttg ttttgtacgg accaagaaat
     5641 caacgaagtc gtaagcactt tgaccaaatg atccgattcc gtaagctcct ggaagagaag
     5701 agatgtgcat caacacacca ctttgacgtt ttttcataat agcacctcgt gtgttaaaat
     5761 aatggaacgt tgcgtatttt gcagacgcaa acgtttgcgt tacttataag tatactcctt
     5821 ttcaagcata attgcaagcg tttttaaaaa acttttttga ttatttttaa ggagactaac
     5881 tcattttagc aaagataact taaaactatc taatcctaag gtttgactct cacgagacag
     5941 tctacaaagt acaaaacctc cttagggagc ttgaatttat atgtaacaaa gcacaaacgt
     6001 ccatagaaaa tagataggga ttagaaccag ggaggtagcc cctcctggtt tccctcttta
     6061 gacagattcc atatattttt attaaaaata ctgtattctt atctatttca tacttttctt
     6121 ctattaaaat gcaaaattat gctatcaaat caagcttaaa aattttttaa aatttttaca
     6181 aaaaatactt gcaaccgttt tctatttgtg ctatactaag ctcataaagg aaaacgtttg
     6241 cgtttcctta tcaataaaac ttgctattct ttgggaggaa tacactatgt catctaaatt
     6301 tatgaagagc actgcggtgc ttggaactgt tacacttgct agcttgcttt tggtagcttg
     6361 cggaagcaaa actgctgata agcctgctga ttctggttca tctgaagtca aagaactcac
     6421 tgtatatgta gacgagggat ataagagcta tattgaagag gttgctaaag cttatgaaaa
     6481 agaagctgga gtaaaagtca ctcttaaaac tggtgatgct ctaggaggtc ttgataaact
     6541 ttctcttgac aaccaatctg gtaatgtccc tgatgttatg atggctccat acgaccgtgt
     6601 aggtagcctt ggttctgacg gacaactttc agaagtgaaa ttgagcgatg gtgctaaaac
     6661 agacgacaca actaaatctc ttgtaacagc tgctaatggt aaagtttacg gtgctcctgc
     6721 cgttatcgag tcacttgtta tgtactacaa caaagacttg gtgaaagatg ctccaaaaac
     6781 atttgctgac ttggaaaacc ttgctaaaga tagcaaatac gcattcgctg gtgaagatgg
     6841 taaaactact gccttcctag ctgactggac aaacttctac tatacatatg gacttcttgc
     6901 cggtaacggt gcttacgtct ttggccaaaa cggtaaagac gctaaagaca tcggtcttgc
     6961 aaacgacggt tctatcgcag gtatcaacta cgctaaatct tggtacgaaa aatggcctaa
     7021 aggtatgcaa gatacagaag gtgctggaaa cttaatccaa actcaattcc aagaaggtaa
     7081 aacagctgct atcatcgacg gaccttggaa agctcaagcc tttaaagatg ctaaagtaaa
     7141 ctacggagtt gcaactatcc caactcttcc aaatggaaaa gaatatgctg cattcggtgg
     7201 tggtaaagct tgggtcattc ctcaagccgt taagaacctt gaagcttctc aaaaatttgt
     7261 agacttcctt gttgcaactg aacaacaaaa agtattatat gataagacta acgaaatccc
     7321 agctaatact gaggctcgtt catacgctga aggtaaaaac gatgagttga caacagctgt
     7381 tatcaaacag ttcaagaaca ctcaaccact gccaaacatc tctcaaatgt ctgcagtttg
     7441 ggatccagcg aaaaatatgc tctttgatgc tgtaagtggt caaaaagatg ctaaaacagc
     7501 tgctaacgat gctgtaacat tgatcaaaga aacaatcaaa caaaaatttg gtgaataaaa
     7561 aatttgttca aggggggtgg aaatcaaatc cccctttgaa tttatcaata gagacacaaa
     7621 taatttagct ttcttataaa aaagtagtat cctatgaaag gagttaatat ggaaaagcaa
     7681 caacctagta aagcagccct gctgtctatc attcctgggt taggacagat ttacaataaa
     7741 caaaaagcca aaggttttat cttccttggt gtaaccatcg tatttgtcct ttacttccta
     7801 gcacttgcaa cccctgaatt gagcaacctc atcactcttg gtgacaaacc aggtcgtgat
     7861 aattccctct ttatgctgat tcgtggtgcc ttccatctaa tctttgtaat cgtttatgta
     7921 ctcttttatt tctcaaatat caaagatgca catacgattg caaaacgcat taacaatgga
     7981 attccagttc cacgcacact caaagacatg atcaaaggga tttatgaaaa tggcttccct
     8041 tacctcttga tcattccatc ttatgttgcc atgaccttcg cgattatctt cccagttatc
     8101 gtaaccttga tgatcgcctt taccaactac gacttccaac acttgccacc aaacaagttg
     8161 ttggactggg ttggtttgac caactttaca aacatttgga gcttgagtac cttccgttct
     8221 gcctttggtt ctgttctttc ttggactatc atttgggctt tggcagcttc tactttacaa
     8281 atcgtaattg gtatcttcac agctatcatt gccaaccaac catttatcaa aggaaaacgt
     8341 atctttggtg ttattttcct tcttccttgg gctgtcccag ccttcatcac tatcttgaca
     8401 ttctcaaaca tgtttaacga tagtgtcggt gctatcaaca ctcaagtatt gccaatcttg
     8461 gctaaattcc ttcctttcct tgatggagct cttattcctt ggaaaacaga cccaacttgg
     8521 actaagattg ccttgattat gatgcaaggt tggctcggat tcccatacat ctacgttctg
     8581 accttgggta tcttgcaatc tattcctaac gacctttacg aagcagctta tattgacggt
     8641 gccaacgctt ggcaaaaatt ccgcaacatc actttcccaa tgattttggc tgttgcggca
     8701 cctactttga ttagccaata caccttcaac tttaacaact tctctatcat gtacctcttc
     8761 aatggtggag gacctggtag tgtcggaggt ggagctggtt caaccgatat cttgatctca
     8821 tggatctacc gtttgacaac aggtacatct cctcaatact caatggcggc agctgttacc
     8881 ttgattatct ctatcattgt catctcaatc tctatgatcg cattcaagaa actacacgca
     8941 tttgatatgg aggacgtcta agatgaataa ctcaattaaa ctcaaacgta gactgactca
     9001 aagccttact tacctttacc tgattggtct atcaattgta attatctatc cactgttgat
     9061 taccattatg tcagccttta aagcaggtaa cgtctcagcc tttaaactag atactaatat
     9121 cgacctcaat tttgataact ttaaaggcct cttcactgaa accttgtacg gtacttggta
     9181 cctcaacact ttgattatcg ccttaattac catggctgtt caaacaagta tcatcgtact
     9241 tgctggttat gcttacagcc gttacaactt cttggctcgt aaacaaagtt tggtcttctt
     9301 cttaatcatc caaatggtgc caactatggc cgctttgaca gccttcttcg ttatggcgct
     9361 tatgttgaac gcccttaacc acaactggtt cctcatcttc ctctacgttg gtggtggtat
     9421 cccgatgaat gcttggctca tgaaaggcta cttcgataca gtgccaatgt ctttagacga
     9481 atctgcaaaa ctagacggtg caggacactt ccgccgcttc tggcaaattg ttctaccact
     9541 tgttcgccca atggttgccg tacaagctct ctgggccttc atgggacctt tcggggacta
     9601 catcctctct agtttcttgc ttcgtgagaa agaatacttt actgttgccg taggtctcca
     9661 aaccttcgtt aacaatgcga aaaacttgaa gattgcctac ttctcagcag gtgctatcct
     9721 catcgccctt ccaatctgta ttctcttctt cttcctacaa aagaactttg tttcaggact
     9781 tacaagtggt ggcgacaagg gataatttat ccccgccacc ctttttcatt ttatactctt
     9841 cgaaaatctc ttcaaaccac gtcagcttta tctccaacct caaagttgtg ctttgagcaa
     9901 cctgtggcta gtttgcactt tgattttcat tgattattag caattgtcac tgtaaataat
     9961 atccttgtag caagcaattt ttctcctaga cttgaaataa agcgcatttc tctatataat
    10021 aatactcata tagaaaacac cttttagaaa gatacctatg cttccatatc cattttccta
    10081 tttttcaagt atttgggggt ttcgtaagcc cctgtccaaa cgtttcgagc tcaactggtt
    10141 tcaacttctc tttaccagta tcttccttat cagcttgtct atggtaccca ttgctatcca
    10201 aaacagctcc caggagacct atccgctaga aacttttatc gataatgtct atgaacctct
    10261 gacagataag gttgtccagg atctctctga acatgctaca attgtcgatg gcacattaac
    10321 ttatactgga acagctagtc aagccccttc tgttgtgatt ggtccaagtc aaatcaagga
    10381 attacctaag gacttgcaac tgcatttcga tacaaatgaa ctagtcatca gcaaggaaag
    10441 caaggaactg acccgcatct cttaccgagc cattcagact gagagtttca aaagcaaaga
    10501 cagcttgacc caagcaattt ctaaagactg gtaccaacaa aatcgtgtct atatcagcct
    10561 cttcctagtt ctcggtgcga gcttcctctt tggtttgaat ttctttatcg tctctctagg
    10621 agctagtctt ctcctttata tcactaagaa atcacgcctc ttttcattta gaacctttaa
    10681 agagtgctat cactttatct tgaactgttt aggattgccg actctgatta cacttatttt
    10741 gggattattt ggccaaaata tgacaaccct gattactgta caaaatattc tttttgttct
    10801 gtatctggtc actatctttt ataaaacaca tttccgtgat ccaaattacc ataaatagga
    10861 gatttttatg cccgttacga ttaaagacgt ggccaaggct gctggtgttt cgccttcaac
    10921 cgtaacccgt gttattcaaa ataaatcaac cattagcgac gaaacaaaaa aacgcgttcg
    10981 caaagctatg aaggaactca actaccaccc aaacctcaac gctcgtagct tggtaagcag
    11041 ctatactcag gttatcggat tagttcttcc tgatgactca gacgccttct accagaatcc
    11101 tttctttcca tcggttctac gtggcatctc tcaagtcgca tctgaaaacc actatgccat
    11161 tcagatagca acagggaaag atgagaagga gcgtctcaac gctatttcac aaatggtcta
    11221 cggcaagcgt gtagatgggc taatctttct ctatgcccaa gaagaagacc ctctcgtaaa
    11281 actcgtcgca gaagaacagt tccccttcct tatcctaggt aaatctctat ctcctttcat
    11341 cccacttgtc gacaacgaca atgttcaagc tggttttgat gcgactgaat atttcatcaa
    11401 aaaaggctgc aaacgcattg cctttatcgg aggaagtaaa aagctcttcg tgaccaaaga
    11461 ccgtttaaca ggctatgaac aggcgcttaa acattacaaa cttaccactg acaacaatcg
    11521 catctacttt gccgacgagt ttctggaaga aaagggctat aaatttagca agcgattatt
    11581 caagcacgat ccacaaattg atgctatcat cacaaccgat agcctcctag ctgaaggtgt
    11641 ttgtaactat attgccaaac accagctgga tgtccctgtt ctcagctttg actcggttaa
    11701 tcccaagctc aacttggcag cctatgtcga tatcaatagt ttagagcttg gtcgtgtttc
    11761 ccttgaaact attctccaga ttattaatga taataaaaac aataaacaaa tttgttaccg
    11821 tcaattgatc gcccacaaaa ttatcgaaaa ataagagact gggcaaaaag tcgttaaaag
    11881 caaaaacgca tactatcagg tattgaaaaa acttgatact atgcgtttta ttgtgggaag
    11941 atttacttcc ttttcttctg aaattgagtt gttacccaag atctttttat actcaataaa
    12001 aatcaaagtg caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgt
    12061 ggatagaact gacgaagtca gctcaaaaca tggttttgag gttgcagata gaactgacga
    12121 agtcagtaac atacctacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag
    12181 tattaatcac taattatcta tctcaacaaa tcttcctaga atatgaacat tttccgagac
    12241 agagacaaag gagcttggat ccacttgtgt cataatctgt ttaaattcat taaactctgc
    12301 acgtgtaatg acagtgatta aaactgcctt tctctcgtga ttataggttc cttctgcatc
    12361 gtggatcatg gttgctccgc ggtgcaattt tttatggatt ttttcaatta ccttctctgg
    12421 atgatttgtc acaatcatgg cctgcatacg cttttgctta gtaaagactg cgtctgtcac
    12481 acggctagag acaaagatgg taatcataga ataaagagcg tatttccaac caaaggtcaa
    12541 acctgctatc agcatgatag ttccatttac caagaaagaa atactaccga cattcttacc
    12601 cgttttcttg cgaatagtca ggctgacgat atccgtccca ccactggaga tattgtttcg
    12661 aagagcaaaa ccaatcccca aacccataac aacaccccca aaaagggaat tgataatggg
    12721 atcctccgtc aaggttgcca cagggacaaa ctggataaag aaggaactca tagataccgt
    12781 gataaaggta aagacggtga acttatggcc aatctgatac caagctaaga ccatcaaagg
    12841 gaagttaatg gcgtagaagc ttagcgaaat cggaatatga aaaccaaacc agtgattact
    12901 caaggcagag ataatctgtg ccagacctgt tgcaccactc gaatacacat gccctggttg
    12961 gaaaaagaaa ttaactgcta ctgctgataa aaaaccatag accagagagg ccgaaatctt
    13021 ctcatcatac ttttctcgag agatactttg taagacacgt aaaattttta tctgataagc
    13081 aaagcggcgc agataatagc gccaccgctt aattcgtttt gtttgtttca tcttcttcta
    13141 cttgtaagct gagttcctct agttgtttga gagcgactgt tgatggagct tgtgtcattg
    13201 gatcagttgc cttgttgttc ttaggaaagg caatgacttc acggatattt tcttctccag
    13261 caagcaacat gacaaaacgg tcaagcccga tagccaaacc accgtgtggt gggaaaccat
    13321 agtccatggc ttcaagaagg aaaccaaact ggtcattggc ttcttcagct gagaaaccaa
    13381 gagccttgaa catgcgttct tgaaggtctt tttggttgat acgaaggcta ccaccaccaa
    13441 gctcataacc gttcaagacg atatcgtaag caatggcacg aaccttagcc aaatcacctt
    13501 ctaattcgtg agcagtctct tcctgtggaa gtgtgaatgg atggtgggcg ctcatgtagc
    13561 ggccttcttc ttcagaccat tcaaacatcg gccagtcaac cacccaaagg aagttgaact
    13621 tatcattatc aatcaagcca agctctttag caatacgtcc acgaagggca cccagtgttg
    13681 cattagccac ttcaagcgta tccgccacaa agagaaccaa gtccttatct tcaagagcaa
    13741 gcgctgttgt caattcttct tggataccag tcaagaactt ggcaactggt ccgtttaatt
    13801 ctccatcaac caccttgacc caagcaagac ctttggcacc atactgtttg gctacttccg
    13861 tcatcttgtc gatgtcttta cgtgaatagt tgtccgcagc tcctttgacc acaatcgctt
    13921 ttacagcaag tgcttctgaa aagactttaa agtctacacc tttgaccact tctgtcaagt
    13981 cctgaagcaa catgtcaaaa cgagtatctg gcttgtcaga accgtaaaga gccatagcat
    14041 catcgtattt catacgaggg aacggtagcg ttacttcgat gccttttgtt tccttcatca
    14101 cacgcgcgat caagccttct gtaatatctt ggatttcttg ctcagtaagg aaggacgttt
    14161 ccaagtcgac ctgagtaaat tcaggctggc ggtctccacg caagtcctcg tcacggaaac
    14221 atttaacgat ttggtagtaa cggtcaaaac cagcattcat caagagctgt ttcgtgattt
    14281 gtggactttg aggaagagcg taaaaatgcc ccttattaac acgagacggc actaaataat
    14341 cacgcgcccc ttcaggcgtt gacttagaaa ggaatggtgt ctccacgtcg ataaactcca
    14401 actcatccaa gtagttgcgg atagagtggg tcaccttggc acgaagttta agattttcca
    14461 acatttctgg acgacgaagg tcaaggtaac ggtaacgcaa acgtgtatcg tcatttgcct
    14521 caatgccatc cttaatctca aatggtgttg tcttagctgt gttaagcaca ataagagctg
    14581 tcacgtttaa ctcaaccgca ccagttggca acttatcatt ggcttgctca cgcgcagcga
    14641 cctgaccagt cacctcaata acaaattcgc tacgaaggct ttcagctgtt gccataacct
    14701 ctgcagatac tttttcaggg ttgataacca actgcatgat tccttcacgg tcacgaagat
    14761 cgataaagat caaaccacca aggtcacgac gacggccaac ccatcctttc aaggttattt
    14821 cttgtccgat gtgttcctca cgaacacgac cagcatacat actacgtttc attatttctc
    14881 tcctctttta ttctgttact attttaccat aaaagcgcag gctcttcatg aaaatcatca
    14941 gaaaagtttg ccagtcttta aaagtcaggt gaaagcccta aaaattagcg ctaatgctct
    15001 tcgaaaatct cttcaaacca cgtcagcgtc gcctt
//
LOCUS       DBERCA010000043        14423 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.043, whole genome shotgun sequence.
ACCESSION   DBERCA010000043 DBERCA010000000
VERSION     DBERCA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.043"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(101..1390)
                     /gene="hisS"
                     /locus_tag="ACL719_001504"
     CDS             complement(101..1390)
                     /gene="hisS"
                     /locus_tag="ACL719_001504"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGS0699445.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1468..2715)
                     /locus_tag="ACL719_001505"
     CDS             complement(1468..2715)
                     /locus_tag="ACL719_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGS0699446.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLPQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGSQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDRFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFFGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(2905..3756)
                     /gene="rgg"
                     /locus_tag="ACL719_001506"
     CDS             complement(2905..3756)
                     /gene="rgg"
                     /locus_tag="ACL719_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGS0699447.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYKLNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3880..4221
                     /locus_tag="ACL719_001507"
     CDS             3880..4221
                     /locus_tag="ACL719_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS0699448.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4520..6223)
                     /gene="ilvD"
                     /locus_tag="ACL719_001508"
     CDS             complement(4520..6223)
                     /gene="ilvD"
                     /locus_tag="ACL719_001508"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGS0699449.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6456..7388)
                     /locus_tag="ACL719_001509"
     CDS             complement(6456..7388)
                     /locus_tag="ACL719_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGS0699450.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7385..8242)
                     /locus_tag="ACL719_001510"
     CDS             complement(7385..8242)
                     /locus_tag="ACL719_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS0699451.1"
                     /translation="MILSKNREDELRKFAANIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPETFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8246..9592)
                     /locus_tag="ACL719_001511"
     CDS             complement(8246..9592)
                     /locus_tag="ACL719_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0699452.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(9605..9889)
                     /locus_tag="ACL719_001512"
     CDS             complement(9605..9889)
                     /locus_tag="ACL719_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699453.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9893..11923)
                     /locus_tag="ACL719_001513"
     CDS             complement(9893..11923)
                     /locus_tag="ACL719_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0699454.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPIIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKVSSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            12114..13121
                     /locus_tag="ACL719_001514"
     CDS             12114..13121
                     /locus_tag="ACL719_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS0699455.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13124..13324
                     /locus_tag="ACL719_001515"
     CDS             13124..13324
                     /locus_tag="ACL719_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0699456.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13475..13657
                     /gene="rpmF"
                     /locus_tag="ACL719_001516"
     CDS             13475..13657
                     /gene="rpmF"
                     /locus_tag="ACL719_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGS0699457.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13673..13822
                     /gene="rpmG"
                     /locus_tag="ACL719_001517"
     CDS             13673..13822
                     /gene="rpmG"
                     /locus_tag="ACL719_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0699458.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14314..>14423)
                     /locus_tag="ACL719_001518"
     CDS             complement(14314..>14423)
                     /locus_tag="ACL719_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS0699459.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         4638 a         3053 c         2485 g         4247 t
ORIGIN      
        1 ttgccgtagg tatggttact gacttcgtca gtttcatcta caacctcaaa acagtgtttt
       61 gagcaacctg cggctagctt cctagtttgc tctttgattt ttattgagta taaaatccta
      121 gtttttcaaa gatttctgag aagttttggc tgattgtctc aagtgacact tgcacttctt
      181 ctcgagtttg gttgttcttg accgtcactt gtccgctttc gacttcgctc tctcctaggg
      241 tgatgagggt cttagccaca aagacatcgg ctgacttgaa ctgagctttt agtttacggt
      301 tgaggtaatc acgctctgct ttgaaacctt gttggcgaag agcctgtacc aattccaagg
      361 ccttgatatt tgccccttcg cccaagactg cgatatagac atctagggcg ttttcgatag
      421 ggagggtcac gccttgtttt tcaaggatga gaagcaggcg ctctacacca agtccaaaac
      481 caaatccagc agtttcaggg cctccaaagt aagcaaccaa accatcgtag cgaccacccg
      541 cacagactgt caagtcattg ccctcaatct ctgtgataaa ctcgaaaatg gtgtggttgt
      601 agtagtccag accacgcacc atattggtat cgatgatgta atctactcca agattttcca
      661 acatctgacg cacagcatca aaatgagttt ggctttcttc atcaagaaag tccaagatag
      721 acggcgcatt ctctactgcc accttgtctt ctttttcctt agagtccaag acacgaagag
      781 gattttcctc caagcgacgt tggctatcct tagacaaggt ctccttgagc ggtgtcaaat
      841 agtcaatcaa ggcttggcgg taggctgcac ggctctcagg atttccaaga gtgttgaggt
      901 gcaatttgac accttgaata ccgatttcct tcaaaaaatg ggctgccata acgattgttt
      961 ccacatcggt agctggattg ctagagccaa aacactcaac accaatctgg tggaattggc
     1021 gcaagcgccc tgcctgtgga cgctcataac ggaacatagg tcccatgtag tagaacttgc
     1081 ttggcttttg cacttctggg gcgaagagtt tattttccac ataggaacgg acaacgggcg
     1141 cagttccttc tggacggagg gtaatatggc ggtcaccctt gtcatagaaa tcgtacattt
     1201 ccttggttac gatatccgtt gtatctccga cagagcggct gataacctcg taatgctcga
     1261 aaataggcgt acgcacttct gcgtagttgt agcgcttgaa aatttcacgg gcaaagccct
     1321 caacgtactg ccacttagca gactcagcag gtaaaatatc ctgcgttcct tttggttttt
     1381 gtaatttcat agggaatcct ctttaaactt aatagtctta ttttaccata aatagaggga
     1441 ttaaaacagt aagaaaaaaa ttaggattta gatatcattt ttgagattaa gaattgttaa
     1501 aaaaatagct agcaaggaaa aaccaacaaa tagcatccaa gtcaactgca tgttccatac
     1561 ggctactagt gaaaaacaag ctgttcccac aggtatggat aaggtaaaca atagaccgaa
     1621 aaaattacta gtacgagcta gaacctctgg agctagattt ttcatgagca tggcactaat
     1681 ctttggttga actttaccag acacatacag agtaaagaag agaaatagca aaccaagcac
     1741 gacttgattg aataaattag ccaaaccaac tagactaagt cctacggtct cccacatcat
     1801 caatctaggc aaggactgct tcccaaaata atcattgccc gtaaggctac tgatgatgat
     1861 tgctactaaa acacagaatt gattgataaa tagtgcctct gtataagaaa aattcaagag
     1921 agaatggctc aaaaagaaga tattataaat tccacccaaa gcgccaccca aggaattaat
     1981 aagcaagaca gcaaagagca taaaaccaaa gtttttctgt ccacttttaa gaaaaacgag
     2041 acgtaaattt cggtaaattg ttaggaaccg gtctttgata gaaagcttct cattttttaa
     2101 gttttcacca tcagcagatg acattgacag gctcaatttg ctttttccta aaaagaggat
     2161 agtggctgat actaggaaaa agcaggcatt gattcccgca acgagagaaa aattattgac
     2221 cgatagagct aagagccaga ctccgaaagc ttgactacca atagctgaaa tataggtgat
     2281 gaactgtgaa aaagaataag cctccatcaa atcatcttca gctacttttt ccttaataag
     2341 aggcatacgc aggccacctg caaaatcact gatgacatca ctaatgacat tgatcaaaca
     2401 caggctagaa aaggcaaaga gactagcttg ctgaacaact agggctgcta gaaaaaatag
     2461 aaccgcctga aacaaaccgc tatagaccat ccatttgacc ttgtccctcg tgtaatctgc
     2521 ccgaatccct gcaaaaactg taaagagggt cggaagaatc atgacaatat tcgccatagc
     2581 aacagcaaaa gatgcttgtg gcaaggtcga tgcatagacg ataaagacca ggttgaaaat
     2641 cgaagcacca aaagcattga agaagcgtga caaagtcaat aggcgataag cttgatttct
     2701 aaacaatagt ttcatagata atctccctcc ttgttctaag ttctacatca agaatagctt
     2761 tagtatagca cttatgaatt tgcgaaacaa ttcttttgaa tatatgcaac aaataaaatg
     2821 agcaaaaaac gatagaactt ttgtaatgac ctgagaaagg gcaacagttc tatcgcttca
     2881 aattttttct tagtttgcag atattcaaca ttcggtcgta tttctatagt attcggcaga
     2941 tttattacag ccaagcatct caaaaatacg gacagcatcc tccatctttt tctggccttc
     3001 cttgactcta ccttgcttgc tatcaaggag accttctgcc cacagataaa caattcggaa
     3061 ataggtctca ttttccttgt agaaatgctc ttcgataaca cgtttaaaat aataggcatt
     3121 ggtaaattct tcacactcaa tactagctaa aaagccattc aatagtatag tatgaaaaag
     3181 gtttcgattg ccagacattt ccattagaaa atcagattta cgtaccattt ctcgtacata
     3241 tctagtaaaa agagaaacag ataaaaatgg agaactgact gaaaataaat tgagtttata
     3301 gattccccag atctcggtag aaaacaaata atcatgaagg acttttcctt cctctgctgt
     3361 taactctacc ctttcatcca tgctcttcat ataagacttg atcataatgg catttagaat
     3421 atgtttctgt ttgttgtgag aatgggcatg cttttgatac tccctgcgat ataagtcctc
     3481 aagaggtgct atattctttg gttccaagac atctgtaatt tcttttctca actcagaatc
     3541 tgtatcatac tggaaacctc tcgccagaaa gaggatctcc tccacactgg cagatatatt
     3601 ttccagagca aatagaaact tttccaccga aagctcactc tgacctgttt caaaacggga
     3661 caacatagac ggcgaaaatt gtcctccggt tgcttgtctc agtgagatat ttcttgattc
     3721 tcgtaattgt ctaaagactt ttccaatctg ctccatagac ttccccttga tcccgtattt
     3781 tcttcatttt atcatatttt tcagaaaatt catcaaaaac ttgccaaatt gtcagaatca
     3841 tgagaaaata gaggatattt atcacgtgga gggactgcta tgagagacga tatcaaaatc
     3901 aatgaccgtg ctttggcctt gcaagaccaa attatcgaaa aactagagaa agtttttgat
     3961 acagatgtgg aattggatgt ttacaatcta ggtctgattt atgaaatcaa tctggatgaa
     4021 acggggctct gcaagattgt catgaccttc accgatactg cctgtgattg cgccgaaagc
     4081 ctgcctattg aaatcgtggc aggtctgaaa caaatcgagg gtatcaaaga tatcaaggtt
     4141 gaagttacct ggtcgcctgc ttggaaaatc acacgaatca gtcgctatgg ccgtattgcc
     4201 cttggactac cacctcgtta agcaggccaa tcacttttaa agatgaaaaa agcaagccga
     4261 aaatggcttg ctttttgata ctcaatgaaa atcaaagggc aaactagaaa actagccgca
     4321 ggttgctcaa aacactgttt tgaagttatg gatagaactg acgaagtcag ctcaaaacac
     4381 tgttttgagg ttgtggatag aactgacgaa gtcagcccaa aacactgttt tgaggttgtg
     4441 gatagaactg acgaagtcag taaccatacc tacggcaagg cgacgttgac gtgatttgaa
     4501 gagattttcg agtatgagtt tattttttac ctgacttgtc catattccag aagtctgtca
     4561 cggctccgcg tgaagcagat gatacgatgt gggcatattt accgaggaca ccacggctgt
     4621 aaagtggtgg caaggttgtt tctgccttgc gtttttcaag ttcttcttcg gatacggcca
     4681 tggaaatttc tttggtatct tggtcaaccg taacgatatc gccggtacgg agataggcaa
     4741 ttggtccacc atcctgagct tcaggagcga tatgtccaac aaccagacca taagtaccac
     4801 cagagaaacg tccgtccgtc aagagggcca ccttatctcc ctgaccttta ccaacaatca
     4861 ttgaagaaag tgacagcatc tcaggcatac caggaccacc tttaggtcca acaaaacgaa
     4921 caacgactac atcgccatca acgatttcat ctgtcagaac ggcctgaatc gcatcttctt
     4981 ctgagtcaaa gaccttagct ggcccaacgt gacgacgcac tttaacacct gataccttgg
     5041 caactgcacc gtcaggagca aggttcccgt tcaagatgat aagcggacca tccgcacgtt
     5101 ttggattttc aagtggcatg ataacttttt ggcctggagt caagtctgca aagtcagcca
     5161 agttttcagc tacagtctta ccagtacatg tgatgcgatc tccgtgaagg aaaccatttg
     5221 ccaacaagta cttcataacc gcagggacac caccgacttc gtagaggtct tggaagacat
     5281 actgaccaga tggtttcaag tcggccaagt gaggcacacg ttcttgaatc gtattgaagt
     5341 cctcaagtga caagtcaaca tttgcggcat gggcaatggc gagcaagtga agagtggcgt
     5401 ttgtagaacc accgagagcc atcgttacag taatagcatc ttcaaaggct tcacgagtca
     5461 agatatctga tggtttgaga ccaagttcca acatcttaac aacagcacgt cctgctgctt
     5521 cgatatcttc tttcttatca gctgattcag ctgggtgaga ggatgaccct ggcaaactca
     5581 tccctagaac ttcgatagca gttgccatgg tattagcagt atacatacca ccacagccac
     5641 cagggccagg gcaggcatta cattcaagac gtttcacgtc ctcagctgtc atgtcaccgt
     5701 ggttccattt tccgatacct tcaaagacag aaaccaagtc gatatcttta ccatcaagat
     5761 ttcccggtgc aatagttcca ccataggcga aaatagctgg gatatccata ttagcaatag
     5821 caatcataga tccaggcatg ttcttgtcac agccaccgat agcgacaaag gcatccacgt
     5881 tgtgaccact catagccgcc tcgatggagt ccgcgatgat gtcacgagat gttagagaga
     5941 aacgcatacc aggcgttccc atagcgatcc cgtctgctac ggtaatggtt ccaaactgta
     6001 caggccaagc gcctgcagat ttgacacctt ctttagccag tttcccgaaa tcatgcaagt
     6061 gaatgttaca tggtgtattt tccgcccaag tcgaaatcac tcccacaatc ggtgtttcaa
     6121 agtccttatc tgtcatacca gtcgcacgaa gcatagcacg gttaggtgat ttaaccatgc
     6181 tgtcataaat gctactgcgg tgacgtttat ctaattcagt catcttatcc ctcccatttc
     6241 agtttttact attatagcac aattttcgca tgaagaacag aataaaaatc ttgaattttc
     6301 agaaaattct atacacatgt gaaatattta aaattaaaaa caacaaagcg gattagtgca
     6361 ctttctgatg accagaatat gctttttaat ccgctttctt taaataacgt actgtaattt
     6421 ttacagaaat tctttcagat aagtgtattt aacatctatc ttgcattata aatttctaga
     6481 accttctctt ttatattcga ttcactcaaa ccatactcat taagaagata atccattttc
     6541 cctacttgac cgaatctttc ttgaacaccc atccgatgaa tttttgtcat tccatcatca
     6601 gagaataatt cacataaaga actgccaatt ccacctatct gattgtggtt ttctacagta
     6661 aatatagttt ttccacttaa cattgttttt atctgttctg gtatcggttt gattctaaat
     6721 aaatctatca cacttactga ataacctaat ttagacagtt catctgcaac tcgaatactt
     6781 ggagcaacca ttataccaga agcaacgatt acaagatctt caccatgcct taactcaatg
     6841 tagcctttag aaaaatcttc tccaccttga tacacaggaa ctggagcttt tctaattgtt
     6901 cgaatatatt ttagtccttt taagtctaat gtctggttca atatttcacg aaattggata
     6961 tcatcagttg cttcaaaaat gatcgattta ggaattaaac gtaacaatcc aatttcttca
     7021 aatggcatat gtgttccacc attcatctct gccgttactc ctgcatctga tccaatcaca
     7081 gtggcatcca attgtgcgta tccaagagaa ataaataatt gatcaaatac tcttcgtgaa
     7141 gcaaaaggac caaatgtatg aagataaggt ctaaacccct gaatagacaa gcctgctgca
     7201 aggccgacca tttctgcttc cataatccca acattcacat aacggtctcc aaaatccttt
     7261 tcaagattat tagtagccat cgaacttgac aaatcggctt ctaagactac tatatcagaa
     7321 taactttgat tagcctctaa aaggaagtct ctatatacat gccgtaattc tttcgtgctt
     7381 ctcatcattc tgtttcctcc aattcctgac ttaatctttc tacaactgaa gttaacattt
     7441 gtctctcctc tacagtaggg cgaagatgat ggttggattt catttcttcc agctcttgaa
     7501 ccccttgacc tttaatagta tctaatacaa tgcacttagg tgatgaatta tttgactgtt
     7561 ttaattggac aatcccttca taaatttctc taatatctga acccttgacc ctaatggatt
     7621 caaatccaaa tgctgaaaat ttttctacga aatcacctgg attacaaata tcctttgtaa
     7681 aaccatctaa ttgttttttg ttatcatcaa caaatacaat taagttggat aactgttgat
     7741 gagaagcaaa ctgtatagcc tcccaacatt gtccctcatt taactcacca tctccaacaa
     7801 tagcataagt ataaaaggga ctctttctta ttctctgacc atatgcaagt ccagttgcaa
     7861 cactaattcc ttgtcctaaa gagcccgttg tcatatctat gcctggcgtt agatttctat
     7921 caggatgaga cggtaatttg gttccatttg tatttaaaga atataagaat tctttgtcaa
     7981 agaaaccatt caaatagagt gtactgtata gagctggtcc tccatgacct tttgataata
     8041 tgaaataatc tctatctcgt gctgcaaatg tttctggagt cattggcatt atttcaccat
     8101 aaagcactgc taaaacttct acgatagaca gacttcctcc gtaatgtccg aatccaagat
     8161 gattcaatgt tctaagagta tttaatcgga tgttagccgc aaattttctt aactcatctt
     8221 ctctattttt acttaaaatc atcccttatt cctcctttgc agatggcttt ttaataaagg
     8281 atactccaaa cataactgct aaaataagaa caagaccaat cacaatgcct gcttgtgagc
     8341 caaattgatt taacattcct aaaataattc ctgatagacc aaaatctgca tctgagaaag
     8401 ttgatccttg gaaaccgagt cctcccaaaa ctggcattaa aaagactggg agaaaactga
     8461 ttaaaatacc ttgtaaaaat gctccaatag tggctccacg aacaccacca gatgcattcc
     8521 caataacacc tgcagtcgct ccacagaaga aatgaggcac aacgcctggt aagataacaa
     8581 ccgttcctga agcaatcata attgccatac ttactaaacc accaataaaa ctagagataa
     8641 atccaattag aactgcatta ggtgcataag tataaacaat cggacaatcc aaagcaggtt
     8701 ttgaattagg tacaagacgc tctgaaatac ctttaaaggc tggaacaatt tcgcccaaaa
     8761 taaggcgaac acctgctaaa ataacaaata cccctgctgc aaattgacct gctaattgta
     8821 aagcataaac tagaccactt gtaccactac tgatttcttt ttctatatat tctgaccctg
     8881 caaagatagc tacaataatg taaataactg ccatagataa agtgatacta acagtactat
     8941 cacgtaaaaa agctaaactc tttggaaatt taatgtcctc tgttgatttt gatttgtcac
     9001 cgataaggct accagtaaaa ccactcaacc aatatcccaa agaactgaaa tgacctaaag
     9061 ccaccttgtc atttccagtt aattgaacca tatatttttg cacaaatgct ggggaaatac
     9121 tcataataat accgagtgct aatcctccta gtaagatgag aggcaagcta gtaaagccag
     9181 caactgataa aatgaccgca atcatacatg ccatatatag agtgtggtgc cctgttaaaa
     9241 aaatatattt aaatcgagta aaacgagcga ttaagatatt gaacaccatt cctgcaaaca
     9301 taatcattgc agtagctgag ccatatgttg ttaaagctac agctacaatt gcttcattat
     9361 tcggcacaac gccagataaa tgaaaagcat gctcaaacat ggtaccaaat ggatttaaag
     9421 aattttgtac aattcctgca ccaccagata caactaagaa accaacaaag gtcttaattc
     9481 cacctttaat aatatcaggt aatttcttct tctgaagaac taatcctaaa attgcaatta
     9541 aagctactaa aatagctggt gtactaacaa tatccaatat gaacttcatc atgacgctag
     9601 cctcctatat aagtcctttt tcttcacaaa gtttagtaat taattctcgt agttcatcca
     9661 tatcaataat actatttaag atacgaacat ctccaagatg actagctgaa tcagctagat
     9721 cacgaccaac aatccaaata tcagctgcat ttggatctgc tccacctaaa tcataatgtt
     9781 caacttctac atccgaaaca ttcaaatcac tcaatacaga ttcaatattc atctgtacca
     9841 taaaacttga acctaatcct gaaccacaag ctgtaccaat ttttaacatt atctaatcct
     9901 cctgtttaat tatcatttta atatcattat agttttttga tgatattaaa gtttgaacat
     9961 gatttttatc tcttaaaatt gttgttaaat gtgacaaagc ctttaaatga ctctcattat
    10021 caatggctgc aatacaaatc aacaatctta cctcttgttc tggattatcc aataaataaa
    10081 tcggttcttc caaaactaac attgacattc ctatttcatt cacaccttca tctggccgag
    10141 cgtgaggaat tgctactccc ttccctaaat taataaaagg tccaaactct tctacttttt
    10201 gaatcattgc ctcagggtag ttctcagtta tcttatcttg atccaaaagc ggtttagctg
    10261 ctaaacgaat cgcctccttc catcctaatt tttgcgaact aacctgatag gtttctttgg
    10321 taataagttg ttctagcact ggtacaattt cctttctatc atttttttga taaagataat
    10381 tctttaacgc caatcttaat tccaattctt gtgtaataat tccatatctt ttgacaatat
    10441 tcaggatttg ttcaatctca aaatctccat actctaaatt cggaaaatct tttaacacta
    10501 gttctactag ttgtattgct tgctcttcag tcatcataac cgaaactaga taatttggct
    10561 tttctgtctc cacctttatg gtagaaaaaa ccatatcata gtcactacta actttcacct
    10621 gtaaatcatc aatctttgag gttcctataa actcaatttg aggaaataat gctaatagat
    10681 tctcttttaa catcaatgaa gaactaacac cattaggaca aatgattgct gctttatacc
    10741 atttttgagg caaagtatct gctttcttta aataacctcc gaaatggata acaaaatatg
    10801 ctgtttcact atcaggtatg ggattgtcaa tagcgtccat caagggcatc aaagaatctt
    10861 tgactaattc aaataaatca ggataatgtt ctttaacatg caatacatat tcatttgaac
    10921 taggtaggcc gaactttaat ctatagtaag ccggtataag gtggcggcga agattttctc
    10981 tcaatccttc cctttgttta aaatgtaaca aagaaatatc ttccattcta cttataatag
    11041 cctctgttaa ttgattaaag taaaccggag caacatctac ttcaccttca aagcaacttg
    11101 ataataaaac ggtgatatag cgataatcat cctcagaaaa caccgtatct ataattccca
    11161 aatcaaccac tgtatccaat aaaatagtcg ttatatcttg aataatagga gatactaatg
    11221 tctctgaaag acacactctt tcaacatccc tttgatacct acacagaatg aatactaaac
    11281 cgaaaaggta aacttttaat tgattaacaa taggtactag ctgtagcttc tcataataat
    11341 ctttaactac ctgatcaatc aaatcataag ttaatgaata cccccaactg gataaaacat
    11401 aatccaaacc ccaaatccct atggaggatt ccagcaactc actaaccatt tgaaaagcta
    11461 agcggtgctt attccactct gaaccgtgta aagtataacc ttttgctcta ctgtacccta
    11521 gctccaaatc attatctaac ataatctttc ttaatgattg aatatcagat aaggttgtat
    11581 tcttacttac tttcaaaaag tcttggtaat gactattcga tataaaatct aatcggcaaa
    11641 aagtgtaaag atagattaaa gctaagcgag tcgactttgg taaaaccaat tcatccgact
    11701 taataatatc tgtcaaagac tgcttcgtac gatttgataa actatagcga ccttgctttt
    11761 tatccagcac tatcccttta ttagctagat aaggcactaa ataatctatt ccttctttga
    11821 cttcctttat aggtaagctc accttaacag ataattcata taatgatagc tcacaatgat
    11881 ccatcaaagt catcaaaata actagtgctc tataatcaaa catctctctt cctttctaac
    11941 tacaattata gtgtaaaaat tataagaata aaagagtatt tgagcacaat ataatctcac
    12001 cttttaaaag aaattattgg gctgaaactt tttagtgatt attttcaact ttctattgac
    12061 ttttaaaaac aaagtgatat catttagata tcaaataatg ggaggtcatt cttatgactg
    12121 aaaaactaat caattcaaaa ccaaatggtg tattcgcatt gattctcatt gagttgacaa
    12181 tcgtacttgg tatctttata tttataatgg gggttggttc ggaaaacatt tttggaatta
    12241 ttatcggacc tttactaatc gtaattgcag gtctagctca tgctggttta aaagttgtca
    12301 aacctcagga agctctggtt ctgacactct ttggtaatta tacaggtacc atcaaagaac
    12361 ctggttttta ctttgtcaat cccttcagcg tagcagtcaa ccctgcaaac cacactcgac
    12421 ttggacaaag tggtgatgtt agcacaaaat ctcctttttt aggagctaaa tcatcaaatg
    12481 acaatgatgt aaatcttgaa attggcaaga aacacatttc cctcaaagtc atgaccttga
    12541 gcaattctcg tcaaaaaatc aatgattgct taggaaaccc tgtagaaatc ggtatcgcgg
    12601 taacttggag agttgtcgat accgctaagg cagtcttcaa cgttgataac tacaaagaat
    12661 atctttcatt gcaatgtgat agcgccctcc gtaatattgt ccgcatctat ccttacgatg
    12721 tgtctcctaa tgtggatact acgggtgacg ggcaagcaga tgaaggtagt ctccgtggct
    12781 ctagcgaaat tgttgctaac cgtattcgtg aagaaatcca aagtcgtgtt gaggatgctg
    12841 gcttggaaat ccttgaagca cgtatcactt acctagctta tgctccagaa attgctgccg
    12901 ttatgcttca acgccaacaa gcatctgcca ttattgatgc acggaagatg attgtagatg
    12961 gtgctgtagg aatggttgaa atggcactag aacggctcaa tgaaggggaa ttggtagaac
    13021 ttgacgaaga acgaaaagct gccatggttt caaatctcct tgtcgttctt tgtggcaatc
    13081 atgatgcaca accaattgtc aacacaggaa gtctttacta aagatggctg aagctaaaaa
    13141 aaagcagatc ccccttcgac tctcaacaaa gttatacgct gcactcgcat catgggcaga
    13201 agacgacttt cgttcagtca atgggcaaat cgaatatctc ctcacagagt gtgtcaagca
    13261 acggaagaaa gacggaaaat acgtatcaga aaccattgac gaaccatttg agattgatat
    13321 ttaacacctt ctcctgtctg ccgtgtcgga taggagaatt ttttcatctt ttttgtcaag
    13381 taactttact ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt
    13441 gaatcgaata taaataccta aaggagaaat caaaatggca gtacctgcac gtcgcacttc
    13501 aaaagcgaag aaaaacaaac gtcgtacaca ctacaaagta acagctccat ctgtaaactt
    13561 tgacgaaact actggagatt actcacgttc tcaccgtgta tcacttaaag gatactacaa
    13621 aggacgtaaa atcactaaag ctgcatcagc tgaataatag aagggagata ccatgcgcgt
    13681 aaatattaca cttgaacaca aagaatctgg tgaacgcttg taccttactt ctaaaaacaa
    13741 acgtaacact ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt
    13801 tgtgtttaca gaagttaagt aagcattcaa tacgaatcaa tacagaagaa aaacgcttga
    13861 tcagcggttt ttctttttgt taatttcact atattgcaag ggaaatcatt cgaatgacta
    13921 catttttcac tacctttttt gaaataagaa tacggattcg gacgattaaa agtaagagaa
    13981 actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc tctacactat
    14041 ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga atatgtaagt
    14101 gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat acaaaagttg
    14161 accaactaga tttctgaata tccttatata tccattctta aaattggttt aaatagcgta
    14221 gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag aagatttttt
    14281 agttcagaat gattagattt agctaatgga tacctatcct acccactcac cgttagcatt
    14341 gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat tataggtata
    14401 gtaccattta ccagaaactg taa
//
LOCUS       DBERCA010000044        13997 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.044, whole genome shotgun sequence.
ACCESSION   DBERCA010000044 DBERCA010000000
VERSION     DBERCA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.044"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(296..1987)
                     /gene="argS"
                     /locus_tag="ACL719_001519"
     CDS             complement(296..1987)
                     /gene="argS"
                     /locus_tag="ACL719_001519"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGS0699460.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2123..2569
                     /gene="argR"
                     /locus_tag="ACL719_001520"
     CDS             2123..2569
                     /gene="argR"
                     /locus_tag="ACL719_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0699461.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2620..5154
                     /gene="mutS"
                     /locus_tag="ACL719_001521"
     CDS             2620..5154
                     /gene="mutS"
                     /locus_tag="ACL719_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGS0699462.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5301..6995
                     /gene="patA"
                     /locus_tag="ACL719_001522"
     CDS             5301..6995
                     /gene="patA"
                     /locus_tag="ACL719_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGS0699463.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SNELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMIQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKNVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7145..7576)
                     /locus_tag="ACL719_001523"
                     /pseudo
     CDS             complement(7145..7576)
                     /locus_tag="ACL719_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7770..9536
                     /gene="patB"
                     /locus_tag="ACL719_001524"
     CDS             7770..9536
                     /gene="patB"
                     /locus_tag="ACL719_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGS0699464.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9675..10364
                     /locus_tag="ACL719_001525"
     CDS             9675..10364
                     /locus_tag="ACL719_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS0699465.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKHLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            10368..10547
                     /locus_tag="ACL719_001526"
     CDS             10368..10547
                     /locus_tag="ACL719_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699466.1"
                     /translation="MEHLVVLFFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10534..11883
                     /locus_tag="ACL719_001527"
     CDS             10534..11883
                     /locus_tag="ACL719_001527"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGS0699467.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEKIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12007..13467
                     /gene="gltX"
                     /locus_tag="ACL719_001528"
     CDS             12007..13467
                     /gene="gltX"
                     /locus_tag="ACL719_001528"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGS0699468.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGTIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13481..13915
                     /locus_tag="ACL719_001529"
     CDS             13481..13915
                     /locus_tag="ACL719_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS0699469.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNEQ"
BASE COUNT         4098 a         2696 c         3148 g         4055 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggcc
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agcgcctagc caaagtccga
      241 ataggatttg gcgttagtta cttagattgc tttgcaatca agtaactttg gcgatttaca
      301 tcttctctgg cgcttctact ccaagcaagc gaagggcttc tttgagaacg actgcggttg
      361 cgtagctgag ggctagacgg ctgtcgcgtt ctgggctttc atccaagata cgtgtatgtg
      421 catagtattt gttaaaggat tgagccaggc taattgcaaa tttagcaatg atagaaggtt
      481 caaagttatc tgccgcacgg ttgataatac gtgggaagtc ttgaatgagt ttaatgattt
      541 cccagctttc agtatcattc aagctatagt tgccagctgt ttctggtttg aaatcggctt
      601 tgcgtaagat agattggata cgagcgtagg catattgaac gtaaggtcca gtttcaccct
      661 cgaaggatac catagcttct aggtcgaagt cgtatccatt tgtacggtca gttttgaggt
      721 catagaattt aatggctcca accccaacag catgtgctac ttggtctttg ttttctagtt
      781 caggattttt agcctcgatt tggaccttgg cacggctaac agcctctgca acagtaggct
      841 ctagcaagat gacattccct ttacgagtag agagcttctt cccttctttt gtaaccagac
      901 caaaaggaac gtgggtaatg tcgtcactcc aatcgtagcc catctcttgc aagacagctt
      961 tgagttgttt aaagtgggca gattgctctt gaccaacgac gtagatagat ttagcaaatt
     1021 ggtattcatt tttacggtag agggctgcag ccaagtcacg tgtgatatag agagttgcac
     1081 catcagactt cttgatgagg gctggatgtt caattccata tttctcaaga ttcacaactt
     1141 gggcaccttc tgattcaaga agtagtcctt tttcagaaag aatgtctaca actgcatcca
     1201 tcttatcatt gtagaaggct tctccgttat agctgtcaaa ttcaaccttc aattcattgt
     1261 aaaggcggtt aaattccact aaactttcat cgcggaacca ttgccaaaga gcgagagctt
     1321 cctcatctcc attttcaagt ttacggaacc attcgcgcgc ttcttcatcc aagctagggt
     1381 cattttcagc ttcagcgttg atgcggacat agagtttaag gagttcatcg attggatgag
     1441 cttttacagc ttcttcgtcg ccccattttt tgtaggcaac aatcaacatc ccaaattgtt
     1501 taccccagtc tcccaaatgg ttgaccttga ccgtttgata accgattttt tggaaaatat
     1561 gtgacaagct atctccgata acagttgaac gcaggtggcc aatagaaaat ggtttagcga
     1621 tattcggact agacatgtcg ataacaacat tttcttgttt gccaatattt tggtcagcat
     1681 agtgttcttt ttcagtggta acagcttgca atacttgagc agaaatggca gatttatcaa
     1741 ggaaaaagtt aacgtaaggt cctgttgcga caactttttc aaaggcttgg ctgttcattt
     1801 tttcagccag ttcagccgca atcatttgtg gtgctttacg ttcgactttt gcaagagaaa
     1861 aagcagggaa agcaatgtct cccatttctg agtttttagg ggtttccagt aactttaaaa
     1921 tagcctcttg gtccaggcta tcaatgatgc tagataattc gctagcaatc aattcttttg
     1981 tattcattaa gagctccttt ttggactttt ctactatttt atcacaattt taaagaaaga
     2041 agaaaaaatt tttgaaatct cctgtttttt tggtataata tggttataaa tatagttata
     2101 aatatgcacg caagaggatt ttatgagaaa aagagatcgt catcagttaa taaaaaaaat
     2161 gattactgag gagaaattaa gtacacaaaa agaaattcaa gatcggttgg aggcgcacaa
     2221 tgtttgtgtg acgcagacaa ccttgtctcg tgatttgcgc gaaatcggct tgaccaaggt
     2281 caagaaaaat gatatggtgt attatgtact agtaaatgag acagaaaaga ttgatttggt
     2341 ggaatttttg tctcatcatt tagaaggtgt tgcaagagca gagtttacct tggtgcttca
     2401 taccaaattg ggagaagcct ctgttttggc aaatattgta gatgtaaaca aggatgaatg
     2461 gattttagga acagttgctg gtgccaatac cttattggtt atttgtcgag atcagcacgt
     2521 tgccaaactc atggaagatc gtttgctaga tttgatgaaa gataagtaag gtcttgggag
     2581 ttgctctcaa gacttatttt tgaaaaggag agacagaaaa tggcgataga aaagctatca
     2641 cccggcatgc aacagtatgt ggatattaaa aagcaatatc cagatgcttt tttgctcttt
     2701 cggatgggtg atttttatga attattttat gaggatgcgg tcaatgctgc gcagattctg
     2761 gaaatttcct taacgagtcg caacaagaat gccgacaatc cgatccctat ggcgggtgtt
     2821 ccctatcatt ctgcccaaca gtatatcgat gtcttgattg agcagggtta taaggtggct
     2881 atcgcagagc agatggaaga tcctaaacaa gcagttgggg ttgttaaacg agaggttgtt
     2941 caggtcatta cgccagggac agtggtcgat agcagtaagc cggacagtca gaataatttt
     3001 ttggtttcca tagaccgcga aggcaatcaa tttggcctag cttatatgga tttggtgacg
     3061 ggtgactttt atgtgacagg tcttttggat ttcacgctgg tttgtgggga aatccgtaac
     3121 ctcaaggctc gagaagtggt gttgggttat gacttgtctg aggaagaaga acaaatcctc
     3181 agccgccaga tgaatctggt actctcttat gaaaaagaaa gctttgaaga ccttcattta
     3241 ttggatttgc gattggcaac ggtggagcaa acggcatcta gtaagctgct ccagtatgtt
     3301 catcggactc agatgaggga attgaaccac ctcaaacctg ttatccgcta cgaaattaag
     3361 gatttcttgc agatggatta tgcgaccaag gctagtctgg atttggttga gaatgctcgc
     3421 tcaggtaaga aacaaggcag tcttttctgg cttttggatg aaaccaaaac ggctatgggg
     3481 atgcgtctct tgcgttcttg gattcatcgc cccttgattg ataaggaacg aatcgtccaa
     3541 cgtcaagaag tagtgcaggt ctttctcgac catttctttg agcgtagtga cttgacagac
     3601 agtctcaagg gtgtttatga cattgagcgc ttggctagtc gtgtttcttt tggcaaaacc
     3661 aatccaaagg atctcttgca gttggcgact accttgtcta gtgtgccacg gattcgtgcg
     3721 attttagaag ggatggagca acctactcta gcctatctca tcgcacaact ggatgcaatc
     3781 cctgagttgg agagtttgat tagcgcagcg attgctcctg aagctcctca tgtgattaca
     3841 gatgggggaa ttatccgaac tggatttgat gagactttag acaagtatcg ttgcgttctc
     3901 agagaaggga ctagctggat tgctgagatt gaggctaagg agcgagaaaa ctctggtatc
     3961 agcacgctca agattgacta caataaaaag gatggctact attttcatgt gaccaattcg
     4021 caactaggaa atgtgccagc ccactttttc cgcaaggcga cgctgaaaaa ctcagaacgc
     4081 tttggaaccg aagaattagc ccgtatcgag ggagatatgc ttgaggcgcg tgagaagtca
     4141 gccaacctcg aatacgaaat atttatgcgc attcgtgaag aggtcggcaa gtacatccag
     4201 cgtttacaag ctctagccca aggaattgcg acggttgatg tcttacagag tctggcggtt
     4261 gtggctgaaa cccagcattt gattcgacct gagtttggtg acgattcaca aattgatatc
     4321 cggaaagggc gccatgctgt cgttgaaaag gttatggggg ctcagaccta tattccaaat
     4381 acgattcaga tggcagaaga taccagtatt caactggtta cagggccaaa catgagtggg
     4441 aagtctacct atatgcgtca gttagccatg acggcggtta tggcccagct gggttcctat
     4501 gttcctgctg aaagcgccca tttaccgatt tttgatgcga tttttacccg tatcggagca
     4561 gcagatgact tggtttcggg tcagtcaacc tttatggtgg agatgatgga ggccaataat
     4621 gccatttcgc atgcgaccaa gaactctctc attctctttg atgaattggg acgtggaact
     4681 gcaacttatg acggaatggc tcttgctcag tccatcatcg aatatatcca tgagcacatc
     4741 ggagctaaga ccctctttgc gacccactac catgagttga ctagtctgga gtctagttta
     4801 caacacttgg tcaatgtcca cgtggcaact ttggagcagg atgggcaggt caccttcctt
     4861 cacaagattg aaccgggacc agctgataaa tcctacggta tccatgttgc caagattgct
     4921 ggcttgccag cagacctttt agcaagggcg gataagattt tgactcagct agagaatcaa
     4981 ggaacagaga gtcctcctcc catgagacaa actagtgctg tcactgaaca gatttcactc
     5041 tttgataggg cagaagagca tcctatccta gcagaattag ctaaactgga tgtgtataat
     5101 atgacaccta tgcaggttat gaatgtctta gtagagttaa aacagaaact ataaaaccaa
     5161 gactcactag ttaatctagc tgtatcaagg agacttcttt gacaattctc cacttttttg
     5221 ctagaataac atcacacaaa cagaatgaaa aggagctgac gcattgtcgc tcccttttgt
     5281 ctatttttta aggagaaagt atgctgattc agaaaataaa aacctacaag tggcaggcct
     5341 tggcttcgct cctgatgaca ggcttgatgg ttgctagttc acttctgcaa ccgcgttatc
     5401 tgcaggaagt attagacgcc ctccttactg ggaaatatga agctatttat agtatcgggg
     5461 cttggttgat tggtgtggcc gtagtcggtc tagttgctgg tggactcaat gttgtcctcg
     5521 cagcctatat tgcccaagga gtttcatccg accttcggga ggatgccttc cgtaaaattc
     5581 aaaccttttc ttatgctgat attgaacaat ttaatgcggg aaatctagtc gttcgaatga
     5641 caaatgatat caaccagatt cagaacgttg tcatgatgac cttccaaatt cttttcagac
     5701 ttcccctctt gttcatcggt tcgtttatcc tagcggttca aaccttacct tctctgtggt
     5761 gggtgattgt tctcatggta gtcttgattt ttggtttgac tgctgtcatg atgggaatga
     5821 tggggcctcg ttttgctaaa tttcaaaccc ttcttgagcg catcaatgcc attgccaagg
     5881 aaaatttacg tggcgttcgt gtggtcaagt cctttgtcca agaaaaagag caatttgcta
     5941 agtttacaga ggtctcaaac gagcttcttg gtcaaaacct ttacattggt tatgcctttt
     6001 cagtagtgga accctttatg atgttggttg gttacggggc ggtcttcctc tctatttggc
     6061 tggtcgcggg aatgattcag tcggatccgt ctgttgttgg ttccatcgct tcttttgtta
     6121 attacctaag ccagattatc tttaccattg ttatggttgg atttttggga aattctgtca
     6181 gccgtgccat gatttccatg cgtcgtattc gagaaattct tgacgcagag ccagctatga
     6241 ccttcaagga tatcccagat gaagagttgg ttggaagtct tagctttgaa aatgtgacct
     6301 ttacctatcc aatggacaag gaaccgatgc tgaaaaatgt gagctttact attgaacctg
     6361 gtcaaatggt tggtgtagtt ggagcgactg gtgcaggaaa gtcaaccttg gctcaattga
     6421 ttccacgtct ctttgatcca caggacgggg ccattaaaat tggtggcaag gatattcgag
     6481 aagtgagtga aggaaccctg cgtaaaacag tttccatcgt tctccaacgt gccattcttt
     6541 ttagtggaac gattgcagat aacttgagac aggggaaggg aaatgctacc ctatttgaaa
     6601 tggagcgcgc agccaatatt gcccaggcta gtgaattcat tcatcgtatg gagaaaacct
     6661 ttgaaagtcc agttgaagaa cggggaacca atttctctgg tggacaaaaa caaaggatgt
     6721 cgattgcgcg tgggattgtc agcaatccac gtattctgat ttttgacgat tcgacctcag
     6781 ccttggatgc caaatcagag cgcttggtgc aagaagcttt gaataaggac ttgaagggga
     6841 cgacaaccat tattattgct caaaaaatta gctcggttgt ccatgcagac aagatcttgg
     6901 ttctaaatca aggacgattg attggtcaag gtacgcatgc agacttggtt gccaacaatg
     6961 ccgtttaccg tgaaatctat gaaacacaga aatgaaagac aaactataag aaaagtcaat
     7021 agttttatct aaactatttc ttatttcaat ttgatgattt ggcgatgatt ttagagcacg
     7081 gcaaaaagcc cttgaaaaag tccatttttt caaaggtaat cctgtgttga tttcagaaat
     7141 tacatcactt tttgttcgtc aaatggcagc tcttttttag gatataaaac agggttcgga
     7201 taagtttttt tgcaaggtgg atgatggcta cattgtaatg ttttccttgt tctaacttag
     7261 tcttaaaagc aggtgaaaag tgagggcatg ctttggcagc ttgtatgagt acctaccgca
     7321 gatgagggga accccgtttg accattcttt ctgctaaatc aatctgacct gactgataaa
     7381 tagaagaatc cagtccagcg aaagcttgta attgagcagg attatcaaag gcatgaatat
     7441 ttcgaatctc ggctaaaatg accgccccta aacgattctc aatcccagta accgtcgtga
     7501 tgaccgagtt gaactcagcc atcaagtcat tgacacattt ttccgccttg tcactgagcc
     7561 tcttgtaatg tttgatgttt tcattacacg agataaaacg tctatgcgtt atcaaactca
     7621 ttaccaatta aaacaaatgt ggttagatcc tttcggaaat tgtcaagcga ttggaggaaa
     7681 tgaactaatc cacagcggct tattccaagt ataccacttg ggctttggca gtagctaact
     7741 gcgctaaata taatataagg aggagtaaaa tgaagacagt tcaatttttt tggcattatt
     7801 ttaaggtcta caagttctca tttgtagttg tcatcctgat gattgttctg gcgacttttg
     7861 cccaagccct ctttccagtc ttttctggac aagcggtgac gcagctagcc aatttagttc
     7921 aagcttatca aaatggcaat ccagaacttg tatggcaaag cctatcagga atcatggtca
     7981 atcttggcct gctggttttg gttctattta tctctagtgt aatatacatg tgtctcatga
     8041 cgcgcgtgat tgcagaatcg accaacgaga tgcgcaaagg tctctttggt aagcttgctc
     8101 agttgacggt ttctttcttt gaccgtcgac aagatggcga tatcctgtct cattttacca
     8161 gtgatttgga taatatcctc caagccttta acgaaagctt gattcaggtc atgagcaata
     8221 ttgttttata cattggtctg attcttgtca tgttttcgag aaatgtgacg ctggctctca
     8281 tcactattgc cagcacccca ttggctttcc ttatgctgat tttcatcgtg aaaatggcac
     8341 gtaaatacac caacctccag cagaaagagg tagggaagct caacgcctat atggatgaga
     8401 gcatctcagg ccaaaaagcc gtgattgtgc aaggaattca agaggatatg atggcaggat
     8461 ttcttgaaca aaatgagcgc gtgcgcaagg caacctttaa aggaagaatg ttctcaggaa
     8521 ttcttttccc tgtcatgaat gggatgagcc tgattaatac agccatcgtc atctttgctg
     8581 gttcggctgt acttttgaat gataagtcta ttgaaacaag tacagcccta ggtttgattg
     8641 ttatgtttgc acaattttca cagcagtact accagcctat tatccaagtt gcagcgagtt
     8701 ggggaagcct tcagttggcc tttactggag ctgaacgaat tcaggaaatg tttgatgcag
     8761 aggaggaaat ccgacctgaa aaggctccaa ccttcactaa gttgcaagaa agtgttgaaa
     8821 tcagtcatat cgatttttca tacttgcctg ataaacctat tttgaaagat gtcagcattt
     8881 ctgcccctaa aggccagatg acagcagttg ttgggccgac aggttcagga aaaacgacta
     8941 ttatgaacct catcaatcgc ttttatgatg ttgatgctgg tggtatttat tttgatggta
     9001 aagacattcg tggctatgac ttagatagtc ttagaagcaa ggtgggaatt gtattgcaag
     9061 attcggtctt gtttagcgga acgattcgag acaatatccg atttggtgtg ccagatgcta
     9121 gtcaggaaat ggttgaggta gcagcaaaag caacccacat tcacgactat atcgaaagtt
     9181 tgcctgataa gtacgatact cttattgatg atgaccagag catcttttca acagggcaga
     9241 agcaattgat ttcaatcgct cgaaccctga tgacagatcc agaagttctc attctcgatg
     9301 aagcaacttc aaacgtagat acggtgacag aaagcaagat tcagcatgcc atggaggtgg
     9361 ttgtagcagg tagaactagt ttcgtcattg cccaccgctt gaaaaccatt ctcaatgcag
     9421 atcagattat tgtccttaaa gatggagaag tcattgaacg tggtaaccac catgaacttt
     9481 tgaagctagg tggtttttat tcagaactct atcacaatca atttgttttc gaataagaaa
     9541 gaagttgtcc tatgtgggca gctttttctt gtccataaaa aatgtttatc acagccttaa
     9601 aaaaaacata ttagacgaaa gtcattttga gtgatatgat aggactatcg ttagcattcg
     9661 aaaggagagg catcatggct agaacggttg taggagttgc tgcaaatcta tgtcccgtag
     9721 acgcagaagg caaaatcatt cattcatctg tatcttgtag attcgcagag atcattcgtc
     9781 aagtcggtgg tctcccttta gtcattcctg ttggtgatga gtcagttgta cgtgattatg
     9841 tggaaatgat tgacaaactc attttgacag gaggccaaaa tgttcatcct cagttttatg
     9901 gagagaaaaa gaccgtcgag agcgatgatt acaatctggt ccgtgacgaa tttgaattgg
     9961 cactcttgaa ggaagcgctt cgtcagaata aaccaattat ggcaatctgt cgcggtgtcc
    10021 aacttgtcaa tgttgccttt ggtggaaccc tcaatcaaga aatcgaaggt cactggcaag
    10081 gactaccttt cgggacatct cactctattg agacagtaga aggaagcgtg gtggctaagc
    10141 tatttgggaa agaaagtcag gtcaactcag tccatcgtca aagcattaaa catttggcac
    10201 ctaatttccg tgtaactgct attgattcaa gagaccaaac tattgaagcg attgagtcta
    10261 tcgacgagca ccgcattatc ggtttacagt ggcatccaga gtttctggtt aatgaagaag
    10321 atggcaattt agaattattt gagtatttat tgaatgaact gtaacgattg gaacatctgg
    10381 tcgttctttt ttttattttt tcaaaatttt tagaatgtgg tattttacgc aaacgtttga
    10441 attctgataa aaattggaga aattcgacaa aaaaacttga aaaaaacgaa ggtaagcgtt
    10501 atgatagaaa agaagaaata ttggaggaag aacatgtcac atattaaatt tgattattca
    10561 aaagttttag acaaatttgt tgcaccacat gaagtggaat acatgcaatc acaagtaaca
    10621 gcagcagatg aattgatccg taaaggaact ggtgctggta gcgacttctt gggatggttg
    10681 gaccttcctg aaaaatacga tcgcgaagaa tttgaccgca tcttgaaagc tgctgagaaa
    10741 atcaagtcag acagcgatgt tttggttgtg atcggtattg gtggatctta ccttggtgca
    10801 aaagcagcaa tcgacttctt gaatcaccat tttgctaact tgcaaacaaa agaagaacgc
    10861 aaagctccac aaatccttta cgcaggaaac tcaatctcat ctacttacct tgctgacttg
    10921 gtagagtatg ttgcagacaa ggacttctca gtaaacgtaa tttctaaatc aggtacaaca
    10981 actgaaccag cgattgcttt ccgtgtcttt aaagaactct tggttaagaa atacggtcaa
    11041 gaagaagcta acaagcgtat ctatgcaaca actgaccgcc aaaagggtgc tgttaaggtt
    11101 gaagcagacg ctaacggttg gggaacattt gttgttccag atgatatcgg tggacgcttc
    11161 tcagtattga cagccgttgg tttgcttcca atcgcagcat caggagctga catcaaagct
    11221 cttatggaag gtgcgaatgc agctcgcaaa gactacactt cagacaaaat ctctgaaaac
    11281 gaagcttacc aatacgcagc cgttcgtaac atcctttatc gtaaaggcta tgcaactgag
    11341 atcttggtaa actatgagcc atcacttcaa tacttctcag aatggtggaa acaattggct
    11401 ggtgaatcag aaggaaaaga ccaaaaaggt atctacccaa cttcagccaa cttctcaact
    11461 gacttgcact cacttggtca atttatccaa gaaggaactc gtatcatgtt tgaaacagtt
    11521 gtccgtgttg acaaacctcg taaaaacgtg cttattccta ctttggaaga agaccttgac
    11581 ggacttggtt accttcaagg aaaagacgtt gactttgtaa acaaaaaagc aactgacggt
    11641 gttcttcttg cccacacaga tggtgatgta ccaaacatgt atgtgactct tccagagcaa
    11701 gacgctttca ctcttggtta cactatatac ttcttcgaat tggcaattgc cctttcaggt
    11761 tacttgaatg ctatcaaccc atttgaccaa ccaggtgttg aagcttataa acgtaacatg
    11821 tttgcccttc ttggaaaacc aggatttgaa gaattgagca aagaacttaa cgcacgtcta
    11881 taatagaaga aaagagtggt ttgcccactc tttttactct ctttatccat agaaattgga
    11941 ctcagccaag acttgtgata taatatagaa agcaaaaagg cagacgccta gataatagga
    12001 gaaactatgt caaaagatat ccgcgtacgt tacgcaccaa gtccaacagg actactacac
    12061 atcggaaatg ctcgtacagc attgtttaat tacttgtatg cgcgccatca tggtggaaca
    12121 tttctcatcc gtatcgaaga tactgaccgt aaacgccatg tcgaggatgg tgaacgttca
    12181 caacttgaaa accttcgctg gttaggcatg gattgggatg aaagtccaga atcacatgag
    12241 aattatcgcc agtctgagcg tttggacttg tatcaaaaat acatcgacca actattagct
    12301 gaaggaaaag cctacaaatc ttacgttaca gaagaagagt tggcagctga acgtgaacgt
    12361 caagaagtag ctggcgaaac accacgctac atcaatgaat accttggtat gagtgaagaa
    12421 gaaaaagcag cttacatcgc agaacgtgaa gcagcaggga ccatcccaac tgttcgtttg
    12481 gctgtcaatg agtcaggtat ctacaagtgg catgatatgg tcaaaggcga tatcgaattt
    12541 gaaggtggca atatcggtgg tgactgggtt atccaaaaga aagacggtta cccaacttac
    12601 aactttgccg ttgttatcga tgaccacgat atgcaaatct ctcatgttat ccgtggagat
    12661 gaccatattg ctaatacacc aaaacagctt atggtctatg aagctcttgg ttgggaagct
    12721 ccagagttcg gtcacatgac cttgattatc aactctgaga ctggtaagaa gttatctaaa
    12781 cgtgatacta atacacttca atttatcgaa gattaccgaa agaaaggtta ccttccagaa
    12841 gcagtcttta attttattgc tcttcttggt tggaacccag gtggtgaaga tgagattttc
    12901 tctcgtgaag aactcattaa actttttgat gaaaaccgcc tcagcaagtc accagcagcc
    12961 tttgatcaga aaaaactaga ctggatgagc aatgattata tcaagaatgc agaccttgaa
    13021 accatctttg aaatggcaaa accattctta gaggaagcag gccgtttgac tgacaaggct
    13081 gaaaaattag ttgagctcta taaaccacaa atgaaatcag tagatgagat tatcccattg
    13141 acagatcttt tcttctcaga tttcccagaa ttgacagaag cagagcgcga agtcatgacg
    13201 ggtgaaacag ttccaacagt tcttgaagca ttcaaagcaa aacttgaagc gatgacagat
    13261 gataaatttg tgacagaaaa tatcttccca caaattaaag cagttcaaaa agaaacaggt
    13321 attaaaggga aaaatctttt catgcctatt cgtatcgcag tttcaggcga aatgcatggg
    13381 ccagaattac cagatacaat tttcttgctt ggacgtgaaa aatcaattca gcatatcgaa
    13441 aacatgctaa aagaaatctc taaataaaga ggatacaata atggacatct gggaaaagat
    13501 gtacgaagaa gcacagaaac tatacaatcc acatgaagta tcagactttg tttatgccaa
    13561 tcatgttgta gctgcagtag aagcagaaga tggacaaata tttacaggat tctgtatgga
    13621 gggaacctgt ggtgttttcc atctctgcgc agaacgggcg gccctcttca atatgtacca
    13681 attttcagga caaactaagg ttaagaaagt attagccttt cgagataaac caccttatgg
    13741 tggaagttca gccatgcctt gcggtgcttg tagagaattc cttttagagc ttaatgctga
    13801 aaataaagat gcagaattca tgatggacta taatataaga aaaacagtta aagtcgcaga
    13861 actaatccct tattggtggg gagaagaacg tgcttctaag tttaatgagc aatagaagag
    13921 tcagtataat tctggctctt tttactataa gtttcaaaaa aaatgaaaaa aattagaaaa
    13981 agtagttgac aaagttt
//
LOCUS       DBERCA010000045        13325 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.045, whole genome shotgun sequence.
ACCESSION   DBERCA010000045 DBERCA010000000
VERSION     DBERCA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.045"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            80..1258
                     /locus_tag="ACL719_001530"
     CDS             80..1258
                     /locus_tag="ACL719_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS0699470.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(1486..1932)
                     /locus_tag="ACL719_001531"
     CDS             complement(1486..1932)
                     /locus_tag="ACL719_001531"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699471.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
     gene            2002..2661
                     /locus_tag="ACL719_001532"
     CDS             2002..2661
                     /locus_tag="ACL719_001532"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGS0699472.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(2713..3864)
                     /locus_tag="ACL719_001533"
     CDS             complement(2713..3864)
                     /locus_tag="ACL719_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699473.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3879..4610)
                     /locus_tag="ACL719_001534"
     CDS             complement(3879..4610)
                     /locus_tag="ACL719_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699474.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            4839..4940
                     /locus_tag="ACL719_001535"
                     /pseudo
     CDS             4839..4940
                     /locus_tag="ACL719_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(4912..5688)
                     /gene="trpA"
                     /locus_tag="ACL719_001536"
     CDS             complement(4912..5688)
                     /gene="trpA"
                     /locus_tag="ACL719_001536"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGS0699475.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIKTEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDITLIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(5681..6904)
                     /gene="trpB"
                     /locus_tag="ACL719_001537"
     CDS             complement(5681..6904)
                     /gene="trpB"
                     /locus_tag="ACL719_001537"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGS0699476.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGREIPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(6882..7481)
                     /locus_tag="ACL719_001538"
     CDS             complement(6882..7481)
                     /locus_tag="ACL719_001538"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGS0699477.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKDGISGTK"
     gene            complement(7468..8235)
                     /gene="trpC"
                     /locus_tag="ACL719_001539"
     CDS             complement(7468..8235)
                     /gene="trpC"
                     /locus_tag="ACL719_001539"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGS0699478.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(8232..9236)
                     /gene="trpD"
                     /locus_tag="ACL719_001540"
     CDS             complement(8232..9236)
                     /gene="trpD"
                     /locus_tag="ACL719_001540"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGS0699479.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNLGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(9247..9813)
                     /locus_tag="ACL719_001541"
     CDS             complement(9247..9813)
                     /locus_tag="ACL719_001541"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGS0699480.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            complement(9810..11171)
                     /gene="trpE"
                     /locus_tag="ACL719_001542"
     CDS             complement(9810..11171)
                     /gene="trpE"
                     /locus_tag="ACL719_001542"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGS0699481.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPVPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSDYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(12312..13313)
                     /locus_tag="ACL719_001543"
     CDS             complement(12312..13313)
                     /locus_tag="ACL719_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0699482.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         3849 a         2998 c         2292 g         4186 t
ORIGIN      
        1 aaaaagatta acatctacat ttatccatag caaaaaatca taggatttta agaatctgac
       61 aactaggaag aaaactaata tgaccttatc taagaaacaa ttacaactaa gagctaaaat
      121 actggaaaca gtttacacat taggtcccat ttcacgaatt gaaatcgcta ctaaaacagg
      181 aataacacct gcaacaacta gcagtatcac aaatgatctg attaaagaaa atattctctt
      241 agaacttggt gaagacgaac acgatacaag cgtagggaga aaaaaaatcc tgctagacat
      301 tcaagctaaa cgattttact acattggttg tgaactatct gaaaaacatt tcacttttgc
      361 tttaggagat aatctaggaa atatcttaaa ggaagagaaa gaaattgtta ctaaacagtt
      421 aatccaagaa aagggaaatc aactcattaa ccagacacta aagcaattct taaataactg
      481 ctctgattat gaaatagaag caattggaat cgcgctacct ggtcgctatt tagatgatta
      541 taaaataaca acaaacaatc ctttatggca acatatcgac ttagaaatga ttcaatccca
      601 ctttgataag cctttgtttt tctctaacaa tgttaactgt atggctatag gtaaacgcct
      661 atttagtcgc caacagaatg accctaattt tgcttatttt cattttgcta gaggtatgca
      721 ctgttcttat atctacgacg gaaacattta tgggaaagga aatctaatga ttggagaaat
      781 agggcataca gttgtttcat ctgagggaga agaatgtagc tgcgggcgca agggatgctt
      841 acaaactttt gctggagaat cttggttaat caaaaaatca aaaattctat atcatcaatc
      901 tccctattct ctacttccta gtttagtcaa aaatgccgat gatattgata tccaagttat
      961 tttaaccgct tatcaattag gtgacactgg aataatcaca cttatccatc aagccttact
     1021 ctatcttagt caaaccattt taaatatcag tatgatgatt gactctcaaa aaatctacct
     1081 acatagtcct ttactaacaa atcaacacat catccaaaaa ctatatagtg aaatgaacta
     1141 caaacccaaa ctgctctata atcgcctacc tgaagtcatt attgaacctt acaatgattt
     1201 tacagccgca cactcagcta ttgcactttg tttgtatcat acaattctac attcttaatt
     1261 ttctattaca tttaataaat aggctaaaaa gagggagtat atcataaaaa gcgaataaac
     1321 ctgttttctg aaaatcagaa ttctgttttg ttcgctttct ttatatttgg aattatactc
     1381 ctatctcccc tttttcataa tgtatagtga aatgaaataa taacaggacg aatcgatcgg
     1441 gacagtcaaa tcgatttcta acaatgtttt agaaatagag gtgtactatt ctagtttcaa
     1501 tccactatat tttcgtacag gtgcttcaac catttgaacg atttcaaatc cttctttttg
     1561 gtaaagattc tgagctcttt gatttgcctc gaagacattt agagaaatac tatctatatc
     1621 ttcattttca aaggccaaac taacaaattt ccttaaagcc tggctaccta agtcttgctc
     1681 ctgtttctgg gggttgataa aaaatctccc gatatgaaga ttgctgtctt ctagcctgat
     1741 tttctggata agtcccacaa actcttgttc atcaaagatt gaaaagactc cttccaaggc
     1801 ttgaaggggc agtagaaaag gaatccttgg tcccatccat tgttcttgaa aggatttgcc
     1861 tagggagttg gaccactggc atacaaattg agcgttttct atactcacct tttcttcaaa
     1921 acgaattgtc atcttttcct caccatctta tctatgtttc tccattatac tatttctccc
     1981 attttttacg aatagataag tatgattgat ttttattttt ttctcgtcgg gagcattcta
     2041 gcttcctttc ttggtttggt cattgaccgt tttccagagc aatccattat cagttcagcc
     2101 agtcactgcg attcctgtca gactcccttg cgtcccttag atttgattcc gattctctca
     2161 caggtcttca atcgctttcg ctgtcgctac tgcaaagttc gctatcctgt ctggtatgcc
     2221 ctctttgaac taggcttagg actcctcttt ctgctttact cttgggaatt gctttccttg
     2281 gggcaagtcg tcctaatcac cgctggtttg accttgggta tatacgactt tcaccatcag
     2341 gaatatccct tactggtctg gatgactttc cacctaatcc taatagcttc ctctggctgg
     2401 aatctggtca tggtctcctt cctcatactt ggaattttgg ctcattttat tgatatccgc
     2461 atgggtgcag gggatttcct ctttttagct tcttgtgctc tcgtctttag cgtaacggag
     2521 ttactgatct tgattcagtt cgcttctgcg acgggtatcc tggcctttct cctgcaaaag
     2581 aaaaaggaaa gacttccttt cgtgcctttc ctcttacttg ctacttgttt gattattttt
     2641 ggtaagctac tacttgtttg ataaattcca atttctgcca tatgtactcc atgaaattat
     2701 ttcacagtta aattataaat tatttctttt gtacaaaggg atgatgttat caaatcgatc
     2761 tgttcttcta tcttcttgat actgatcaaa aaatttcatt tcgactgaaa atatttcgct
     2821 tataaactgt aaacgaatac tttgtttaga cattatagtc gctagactga ctagatgatt
     2881 actcaaaacg acgtccagaa tactctttac tttgcttggt tttttaacaa aaatttgatc
     2941 atccaaaggt tcaatcattt tgtaaccttt ttgcgcaatt tgacgataaa agtaagaatg
     3001 ttgctttgga gtcaataatc ctaacttaaa agctcgatac tctaaagcct gtatcgaaac
     3061 attcaaatcc gacttcaata aaatataact atcaggattg ctgacacgct tgccaaccct
     3121 ctcttcaaat ttgactaaaa actcttcttt tggcaataaa aaacatgatg caaaataatt
     3181 tgcttcttgc tccaaacgat cgccatcttc attcatatct ttatatttat gtaaaagaat
     3241 atgtcctagc tcatgagcta aatcaaaatt tcgacgtaca gatgatttat tcgttcccaa
     3301 cacaatataa ggtcttccca attttgacca tgcgctataa gcatcagctt ggccattaat
     3361 taatcgttcc acgatataga tgcctgaacg ttctaattta taaagcaaat catgattatc
     3421 ttttgaaata cctaattttt ccctggcata aagagccaat tcctcaatgg attctccctt
     3481 atgataagat tcactcacta cattacttag gtcatgaatt gtaatattag gtataattac
     3541 aaaactttca aaataatcaa tcaaactatc taccttatgt aaatacatag tttgaatatc
     3601 tattgttttc cgggttgcta ggtctgcatt tctaaaggca attacagaag aatcaaatcg
     3661 aatgctctct tcttcctgtt caaaataagt taaatcaaca tgaaattggt tggccaaatg
     3721 cattttggtt gataatttag gtttcgtttc gttggactca aactgccaaa tggcttgttc
     3781 cgttaaatta attctctgag ctaattctgc tctacttaaa ccatttaaca gccgtaattc
     3841 tttcaatacc cgaccattaa acatttacat actccttact acttttgacc ttcttgtttt
     3901 tctattcttg gaataatttc aaaatcttct gtttccgata attctgaaaa attaggaata
     3961 tcttgatatt tagcttcttc gaaatggtac gaacttgatt gaatatactc agacaaatct
     4021 tgaatcaagt gtaactgacc agttcgtgca tcaggcataa caacatctac agatataata
     4081 ttgttttctg agtccgcctc ataagttaaa atcataaatt tttcgatatt cgaattttta
     4141 gtagcttgtt caatttcttg aatcatttca tcagaaacta actccatctg aattggaaag
     4201 gaatgactat cttcatcatt tttgtaggaa gaatgttgat taagataaag tgtattcatc
     4261 tgagcatatt caaataagta gccactctta tttttttgta ccaaaggaaa ttggttcgta
     4321 agtcgcttct taccctttat aattaacaat actttcccat atttttctgt gtttgtttca
     4381 aattctaaat atccccaagt ctgtcctgct aattgtaatt tatactcaaa caaatctgct
     4441 gatgcaaatg cagtatcaat atgattaggt cgcgtccatg cataaccatt cgacactatc
     4501 attgtctctc ttttttctag acgttcatct acataatctt tttgcccttt catcaaagta
     4561 tctacaattt tttgtgcctc aagcgaatca aagagatcct gattcaacat aattcttcct
     4621 cctccaaata ctttttaatg aattatatca ttttcttaaa gaaattacta caataattag
     4681 ctttttctta aagttctgtg tcagagtaat ttagaaaatt atatcttcta tagtaaaatc
     4741 aattaaaaac tgaacaaatt tattgggaaa ttcaaatcac tttctaaaaa tattttagga
     4801 accgtagtgt aatattccag attcaattta ctataaaact agcctttctc ctgcaaaaga
     4861 aaaaggaaag acttcctttc gttcctttcc tcttacttgc tacttgtttg attatttttg
     4921 gtaagctact gcttgtttga taaagtcctg aatcggctct ccttggtgga gagcttttac
     4981 tattttcgaa ccgacgataa caccatctga caccgcattg aagcgttcca gatcggcttg
     5041 actagataca ccaaaacctg tcaagactgg gatgtcggcc acttgatgaa gttgcgccaa
     5101 gtgcttgtcc aaatctgcac ggtaattgcc tgatttccct gtcactccat tgatggcaac
     5161 ggcatagacg aatccctccg ccccttcaat caactctttc tggcgctcaa ttcctgtggt
     5221 caagcttact aaaggaatca aggtgatatc tgtatctgcc aaaaatggtt ctacaaagtt
     5281 ggcatgttca tgaggcaagt ctgggataat caagcccttc acagctgtat cagccagatc
     5341 tttgacaaag ttctccacac cgtactgaaa gagggggttg aagtaggtca tgatgaccag
     5401 tggaatctct gttttaatgg ttttcaaggt ttcaactaaa gcctgggtag aggtcccgtg
     5461 ggctaaactg cgcaagccag cttcttcgat aacaggtcca tctgcaacag ggtctgaaaa
     5521 gggaataccc acttcaattg cagagacacc caaatcttct aaaaagtgga ttgtttctcc
     5581 tagtccatcc aaacctttct cgtggtctcc agccatgata tagggaacaa aaattccttt
     5641 tccagctgct tttatagcat ttaatttttc tgttagtgtc ttaggcatga gcttctccct
     5701 tctttgctgc atctgcttcc aagcggtctt tgacttgaac cacatccttg tccccacgac
     5761 ctgataggca tacaatcata gacttttctg gtccaagttc tttggccaat ttcaccgcaa
     5821 aagcgatagc atggctagat tccaaggctg ggataatccc ttccacacga gacaagagtt
     5881 gaaatccttc caaagcttct tcgtctgtca cagggacata gctggcacgt ttgatatcgt
     5941 ggtagtgaga atgctctgga ccgataccag gatagtccaa acctgctgag atagagaagg
     6001 cttcaagaat ttgaccatgg gcatcttgga gcacatccat gagagaaccg tgaagaacac
     6061 ccggacgacc cttggtcaag gtagcagcgt ggtgctctgt atccacacca agcccagccg
     6121 cttcagctcc atacatggct actgactcat cttctacaaa gggatggaag agcccaatag
     6181 cattagatcc accaccaaca caggctacta gggcatctgg cagatctcga cctgtcaagt
     6241 cacggtactg ttgtttagcc tctcgaccga tgacactttg gaagtcacga acgatttctg
     6301 gaaatggatg aggccccaag gcagaaccaa ggatatagtg ggtatcgtcg atattagcca
     6361 cccatgaacg aagggctgca ttgaccgcat ccttgagcac gcgcgaacca tctgtcacgg
     6421 cctcgacctt agctcccaaa agctccatac ggaagacatt gagggcttgg cgtttgacat
     6481 cttcctcacc catgtagatg gtacattcca tgttaaagag ggccgcagca gttgcagttg
     6541 ccacaccgtg ctgaccagca cccgtttctg cgataatttt ctttttaccc atgcgtttgg
     6601 caagccaaac ttgtcctaag gcattgttaa tcttgtgggc tcctgtatgg ttaaggtctt
     6661 cccgtttgag ataaatcttg gctccgccga tatgctgggt caaatttttt gcgtagtaaa
     6721 ggggaatttc acgtcccaca tactggcgca aaagttggtt taattcctct tggaaacttg
     6781 ggtctgcctg actttcacgg taggccttct ccaactccaa aactgctgtc atcaatgttt
     6841 ctgggacaaa acgtccgccg aattttccgt aaaatccatc tttatttggt tcctgatatg
     6901 ccatccttta ccctctctat aaatcttcta atcttttcat gatctttttg tccatctgtc
     6961 tccactccac tcgatacatc tactgcatag ggagtaaaat gttgaattgc ttttactaca
     7021 ttatcttcat taaggccacc tgcgataaag aagggctgtg ctagtccagt cgtatccagt
     7081 tgaccccaat caaaggactg gccacttcct gccacagggg catcaaagag tagataatct
     7141 gcctgagaat tggggacatg cccatttcca tctacctgca cagcctgaat actggcacaa
     7201 ggcaaattct caaataaatc atctgccacc tgaccgtgaa cttgaaccaa gtccaagcca
     7261 actttgtcaa tcgcttccag cagttctacc cgacttggtg aaacaaatac tccaaccttt
     7321 ttcacatctg caggaataag ctttgccaac tcagctgcct cttctaaagt cacctgtctt
     7381 ttactaggtg caaagacaaa accgatatag tcggctcctg ctgaaacggc tgtttccacc
     7441 gcttctttgg tcgatagtcc acaaatttta acctttgtca atctgcaact ccttgattct
     7501 ctgggccaca ttttctgcct gcataagagc tgtccctacc aaaattccgt taaagtatgg
     7561 ggctagtcgt tccgcatcct gccctgtgaa aatggcagat tcagaaatgt aatagcgacc
     7621 ttcctcaaag taaggggcta aatctacact ggtctgcaag tcgacctcaa aggtagtcaa
     7681 gttgcggttg ttgaccccga taatctcagc accaagtctg tgggctacct ctagttcagc
     7741 tagattgtga gtctccacta agacttccag accaagctct gtcgcgtagt catacagttc
     7801 cttgaggcgt tcttcggaca aggctgccac aataagcaag ataactgtcg cacctgcatt
     7861 gcgagcgcgg atgatttgct tttcatctat gataaagtct ttgttgagcg tcggaatctc
     7921 tacctgactg gaaatttccc gtagataatc caaatgccct ttaaagaaaa cctcatctgt
     7981 caacaccgaa atcatcactg ctccgttttc ttcataagtc tgggcctgtt gcacaatatc
     8041 cacatcgaga ttgatatctc ccaaactagg gctagctttc ttgacctcag cgattacctg
     8101 caagcggtcc tgatgattct tcaaaaattc tgccaagcga taggtctggc gcagaggctg
     8161 gatttgctcc agcttcatct gctccacctc acgcgccttc tgctctaaga ttcgtgctaa
     8221 aaattcctga ctcatttttg gtactcctgt aacagtctga gtttttcaag ggccttgcct
     8281 ctagcaatca cttgacgggc caaggcaact ccttccttga tgctatcaat cttaccatta
     8341 gcatagaaac caagaccagc attcaagact gtcgtttcca agaatggact tgcttcgttt
     8401 ttcagaacgc taagcaaaat ttctgcattt tcctgagcat tcccaccacg aatatcttcc
     8461 atagcatagc cttccattcc caaatcctct ggagtaaagc ttgacaagct gatttcgcca
     8521 ttttcaagaa gtgcaatctt ggttgttccg ttcaagccag cttcatccaa cccttctggt
     8581 ccagcaacca cgatggcacg tttgcgaccc agatttttca aaacctgagc tgtactttct
     8641 aggagttctg gacgactaat tccaagaagc tgtgtttcta aagccattgg atgaatcagt
     8701 ggaccagtca agttcataat cgttggaatt cccaattcca aacgagctgg catgatgtat
     8761 ttcatagctg ggtgcatatt tttagcgaag agaaagacga ttccagtttt atcaaagacc
     8821 ttacctagtt cagctggttt gagatcaaga ttgattccca aggcttcgag gacatctgcg
     8881 gaaccagatt tagaagaaat cgagcggtta ccatgcttgg ccatgtgaat accgccacca
     8941 gccaagacaa aggctgcagt tgtggaaata ttaaaactga aagacttgtc cccacctgta
     9001 ccacagttat ccatggcatc atgaatctca gttggaatat gctgggcatg tcctctcatg
     9061 acttgggcaa tggctgtgcg ttcttcaggt gtttccccct tcatcttaag agctaagagg
     9121 agagaagcaa tctgcgcttc agttacacgc ccagttacga tacgctcaat gacatccgtc
     9181 atttccacac ctgataaatt ttcaaatttt gctagttttt caataatctc tttcatccta
     9241 gtttcctcac tttacaacct cctcgataaa attccgaata gaagacaagc cgtctggcgt
     9301 tccaatgctc tctggatggt actggaagcc ataaatcggt aggtttttat gttgaatccc
     9361 catgatggct tggtcatcag tcgaacgagc tgtcacttca aagtcttctg gcatttcctc
     9421 aatcaaaata ctgtgataac gcatgaccgc acggccatcc tcaatacctt gatacaaaac
     9481 agatggcgct tcaaagttga tattgctctg tttcccatgc atgacttttg gagccaaacc
     9541 tagcttacca ccaaagactt ctgcaatggc ttggtggccc aaacaaatcc ctagaatcgg
     9601 cttcttgcct gcaaaatcac gaatcatgtc ttccatcttt ccagcatcaa ctggccaacc
     9661 aggaccagga gaaaagacca gaccatctgc tttttcagct tcttcataca gcttggaatc
     9721 atcatttctc aaaacctgaa cttctgcaaa attcccaatg tattgggcca agttataggt
     9781 aaaagaatca tagttgtcaa tcaataaaat catggtctta gttctccaat tctagtcata
     9841 gattttgctt tgttaatggt ttcttggtat tcgttttggg cgatagagtc gtagacaatc
     9901 cctgccccag cctgcacata ggctctttga tttttgagaa tcatggttcg gatggcaatg
     9961 gccaaatcca tatcacccgt cgcagacaag tagccgattg ctcctgcata tacgccccgt
    10021 ttttccgttt ccagttcata gatgcgtctc atcgctcgaa tttttggtgc tccagaaacg
    10081 gttccagcag gaagcgttgc tttcaaggca tccatagcag tgagttctgc aagcaaacgc
    10141 cccttgacca cactggtcaa atgcatgacg tagcggaaga gctccacctt catatactta
    10201 gtaacttgga cactggccgt ttcagagatg cggccaatat cgttacgccc caagtctacc
    10261 aacattcgat gttctgctgt ttccttctca tcagagagga ggtcagtcgc caaggtcttg
    10321 tcttcttcat ccgtagcccc tcttggtcgc gtccctgcaa tcggattggt tgtcacgatg
    10381 ccatttttga cagaaaccaa actttctgga ctagctccga tgatttgata atccccaaaa
    10441 tcatagaaat aaaggtaatc agaaggatta gtcacgcgga gatttctgta gaagtcaaat
    10501 ggatttccag taacttctgc tgagaaacgc tggctgagta cacattggaa catatctccg
    10561 ttacgaatca agtcacgagc tgtttctacc attccctcaa acttatgtgg agcgatatgc
    10621 ggtttgaagt ctaacggaga tagatccaag tcttcaaatt catttggaac aggaatgcgt
    10681 aattcctcaa gcacttggtt caatgatttt tccaaggcct cttgactgcg ctcactataa
    10741 agtgcatcct ctatgacatg tatcttctcc ttcttgtggt caaagaccat atagctctca
    10801 tagacaaaga aatgcatgtc tggcgtccca attgtatcct cagggatttg accaatttct
    10861 tcataaagcg aaatcatatc gtaacccaca aaaccaatgg ctccaccacc aaaagggagc
    10921 tctgagtggt gctgactctt atgaatcact tcataaagga aatccaaggg atcccgatca
    10981 atcacttgac cattttgata gagaactcca ttttcaaact taatctcaaa aactggatta
    11041 taggctagga tagaaaaacg agccgtttcc ttgtctctcg gaatactctc taaaataacc
    11101 ttatgttgcc cctttaagcg catataagcc aagattggtg ataagacatc tccatgaatg
    11161 attcgttcca ttgtaatttc cctttcagtt ctaattctag ttcgtggcga ctgtatgaaa
    11221 aatccccacg caaaataact tgcgtgagga cgaaattcgc ggtgccacct caattatagg
    11281 atttctccta tctctcattc ctgtctcaga tacctcctgt aacaggctgt gcgataaagg
    11341 gcactccctt gagaatgatg ttttcttctc tcgtttcaga tgaacccaac tttacagctt
    11401 tctctgcttg ttttcagcaa ccacaagctc tctgtgagag aaaagactgt aatttttcca
    11461 tctattattt tttagcttct agtaatctgc aatcgcagct aggtccttgc ctccacgacc
    11521 agagacattg atgaagagat gttcatctcg gtacaccttt atactcttcg aaaatctctt
    11581 caaaccgcgt caacgtcgcc ttgccgtagg tatggttact gacttcgtca gttctatcca
    11641 caacctcaaa acagtgtttt gagcagcctg cggctagttt cctagtttgc tctttgattt
    11701 tcattgagta ttactagctt ttttcgtatt agtccagcct ttttgtttgc ttttagtagt
    11761 aggcaaggag ctgtagatag aactcaagtt catcaaagcg acttaaggcc ctaataaaag
    11821 ataaaccaaa cgacggatag aaaaaagccc acacacagaa tatacttccg tgtgagggcg
    11881 ttggtaacgc ggtgccacct caattataaa gggactatcc ctttacatct ctgccttgtt
    11941 taacaacaag ctgcactgta aggtgtgcgc accgaatttt cattgtttca aattcatttt
    12001 caaaatcagc ccactttcac tacttccaac cacctattca caatcaccac aggctccctg
    12061 aagatcaaaa atagttactt ttctgatttg ttgaacttat tttaatactt tgttttttct
    12121 ttgtcaagac ttttttacga tttttttgaa aatatcattc gaatgtgacc atgtcttcct
    12181 tagatcgaac atgaacatgt cccacttgtt agaaattgga tccaactcaa tagaaactga
    12241 atggaggcta aacagaactt attttagaac actccatctt ttccactagg attttcaaga
    12301 attaaacaat actagaaact ctgtctccta acaaatttag gagaaacttc aacagatgtg
    12361 acactttccc ctttaataat tgctaaaaca ccttctatca tttctttagc caatttaaca
    12421 taattgggag caattgtaga caaagctgga gtataatact gagaaatagg aatattatca
    12481 aatccaatga tagaaatatc atctggaata agaattcctt tctcatagca cgcacgaatc
    12541 aagccctgaa ccttttcatc tcctgaaaca aaaataatgt ccggataatt ttgggtagtc
    12601 aagtgctgca ttgcataaga ataaactgaa tcaattgtag ataagccata aatgactttt
    12661 aaatccataa agtaattttt atcattcaga aaagaacgca cacctctttc acgatcctta
    12721 ttaacatggg attctcctcc cataagcaac cacatatttt taaatttttc ttcagttaca
    12781 gctttcatca tatcataagt agcttgaaaa ttattattag atacatagac tactccagac
    12841 gtttgagatt caccgaaaac aagaaaaggc atatggttct tctttaaata ctgaattctg
    12901 atatcatcta cactttcata aaaaacaata acaccatcta ctaggctacc tgtgcttgat
    12961 ataattgaat tactaattgt atcctcctct ccaaagtact caactatagc attaacacca
    13021 aattctttac acgtccgtaa cactttatct aacagcgtat gaaaccaaat taaaggaaaa
    13081 gagtcgattt tttttacaga aatcaatata tttatagctt cttttttagt taaatttttt
    13141 gcatacgcat ttggaatata cgacaattcc tctataactt tttgaatcgc ttgataagtt
    13201 tcttctttaa catttactcc accattaata actcgtgaaa ctgtttttgg agaaaaacct
    13261 gataaacgtg caatatcata aatagttacc tttttcccat ttatattttt catttcagtc
    13321 ctcca
//
LOCUS       DBERCA010000046        12839 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.046, whole genome shotgun sequence.
ACCESSION   DBERCA010000046 DBERCA010000000
VERSION     DBERCA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.046"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            370..717
                     /gene="ssrA"
                     /locus_tag="ACL719_001544"
     tmRNA           370..717
                     /gene="ssrA"
                     /locus_tag="ACL719_001544"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(887..974)
                     /locus_tag="ACL719_001545"
     tRNA            complement(887..974)
                     /locus_tag="ACL719_001545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(938..940),aa:Ser,seq:gga)
     gene            1136..1876
                     /locus_tag="ACL719_001546"
     CDS             1136..1876
                     /locus_tag="ACL719_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0699483.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1873..2784
                     /gene="pfkB"
                     /locus_tag="ACL719_001547"
     CDS             1873..2784
                     /gene="pfkB"
                     /locus_tag="ACL719_001547"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGS0699484.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2781..4733
                     /locus_tag="ACL719_001548"
     CDS             2781..4733
                     /locus_tag="ACL719_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS0699485.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4870..7122
                     /locus_tag="ACL719_001549"
     CDS             4870..7122
                     /locus_tag="ACL719_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGS0699486.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAVFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKEIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(7166..7600)
                     /locus_tag="ACL719_001550"
     CDS             complement(7166..7600)
                     /locus_tag="ACL719_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGS0699487.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            7763..9847
                     /locus_tag="ACL719_001551"
     CDS             7763..9847
                     /locus_tag="ACL719_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699488.1"
                     /translation="MFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKSGVGKSSLL
                     NKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLINDLTVFENLR
                     LALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARALVTGKTLI
                     LADEPTSSLDKENREKISALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLHFKDHKLVG
                     NCLILNSDEGIKDSKKWSSCLSSSHTKLSVLKHRRPFLPRLLAFFISLVVAIAVTSIN
                     FQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSISTSQNIQT
                     VNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLYQTNTTQRQ
                     IEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEKEGNILKGD
                     GGLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQHPMTKMALD
                     GFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNKGLTYIRQF
                     LMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLKELIGYGKT
                     ISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISGLLPIYTTC
                     RQTVVDTIRKNG"
     gene            9857..10234
                     /locus_tag="ACL719_001552"
     CDS             9857..10234
                     /locus_tag="ACL719_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699489.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            10394..11536
                     /locus_tag="ACL719_001553"
     CDS             10394..11536
                     /locus_tag="ACL719_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS0699490.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEVLEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            11545..12759
                     /gene="thiI"
                     /locus_tag="ACL719_001554"
     CDS             11545..12759
                     /gene="thiI"
                     /locus_tag="ACL719_001554"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGS0699491.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         3801 a         2348 c         2823 g         3867 t
ORIGIN      
        1 cgattcgtcc tattcttatt tcattttact atattttggt gcaataagtg aaaagtagtc
       61 cgaataatat aaggattgat tttatagttt ttaaactcaa atgaattaaa ataaagagag
      121 tacgaaaatt ctcatctgaa agtattttag aataattctc ttcgtgaatt tcttcaaaac
      181 agatagcttc atcttaggta tgtgttaatt ggatttcgta agctctattt acaatcgtaa
      241 agttatgcac tgagaaagtt gtagttagtc ttccagtttg ctaaatttct cgaatagtat
      301 cttttttagg tatgtgattt ctttttgcat ttttgagtta gataaggtat aatggtttta
      361 ttgtcttttg gggtcgttac ggattcgaca ggcattatga ggcatatttt gcgactcgtg
      421 tggcgacgta aacgctcagt taaatataac tgcaaaaaat aacacttctt acgctctagc
      481 tgcctaaaaa ccagcaggcg tgacccgatt tggattgctc gtgttcaatg acaggtctta
      541 ttattagcga gatacgatta agccttgtct agcggcttga taagagattg atagactcgc
      601 agtttctaga cttgagttat gtgtcgaggg gctgttaaaa taatacataa cctatggttg
      661 tagacaaata tgttggcagg tgtttggacg tgggttcgac tcccaccggc tccattattc
      721 ctttgcattc ttttgcattc cttggtaaaa cgttgttaaa tcaacgtttt ttatttttat
      781 ctttggtatt cctttgcatt cttttgctaa aaagggagtc acaaacagac cctattttaa
      841 aaaaggatac aacatttgtt gcatcctaaa aataatcttt tttcgacgga agacatggga
      901 ttcgaaccca cgcacgctat tacacgccta ccgcgtttcc aacacggcct cttaagcctc
      961 ttgagtaatc ttccaatact tactcaaata gtctaccata aaggctctta tcttgcaata
     1021 aaaattctag aaataagaaa aatgatagat tttgaaagaa aatgataaaa aatgcttgac
     1081 ttcgaaataa agtatggtag aatgaatagt gtaaacgata acaggaggtg attcagtgtt
     1141 aaaaacagaa cgtaaacaac tgattctaga ggagttaaat caacatcatg tagtttctct
     1201 agaaaaatta gttagtttgc tagaaacgtc agaatcaacg gttcgaagag acttggatga
     1261 gttggaagcg gaaaacaagc ttcgtcgtgt gcatggtgga gcagaactcc cccactcctt
     1321 acaggaagaa gaaaccattc aagaaaaatc tgtcaaaaac cttcaagaaa agaagttgct
     1381 ggctcagaaa gcagcctctc tcattaaaga aaaagatgtc atctttatcg atgctggaac
     1441 aacaactgct tttttgattc atgaattggt caataagaat gttacagttg tgaccaactc
     1501 cattcaccat gccgctcagt tggttgaaaa gcagattcca actgtcatgg ttggaggaaa
     1561 cgtcaagacg gcgacagatg ctagtatcgg gggcgttgct cttaaccaga ttaaccaatt
     1621 gcactttgac cgtgccttta tcggaatgaa tggtgttgac gatggctatt atacgactcc
     1681 tgatatggag gagggagctg tgaaaagagc tattttggag aatgccaagc agacctacgt
     1741 cttggtggat tcgtcaaaaa ttggacaaac ttgctttgcc aaggtagcac cactcaaacg
     1801 cgctatcgtt atcactagtc aagggcatga gctcttgcag gttattaagg agaaaacgga
     1861 ggtaatagaa gtatgattta tacagtcaca ctcaatccat ccattgacta tatcgttcgt
     1921 ttggaccaag tcaaagttgg tagtgtaaat cgtatggaca gtgatgataa gtttgctggt
     1981 gggaaaggaa tcaatgtcag ccgtgtcttg aaacgtttga atataccaaa tacagcgacg
     2041 ggatttatcg gtggctttac tggtaaattt atcacagata ctttagcaga ggaagaaatc
     2101 gagacacgtt ttgtccaggt ggcagaagat actcgtatca atgttaaaat caaagcagac
     2161 caagaaacag aaatcaacgg aacgggtcca actgttgaat cggttcagct agaagaattg
     2221 aaagctattt tatctagtct gacagcagaa gatacagttg tctttgcagg ttcaagtgct
     2281 aaaaatctag gcaatgttat ctataaggat ttgatttcct tgacgcgcca gactggtgcg
     2341 caagtggtct gtgactttga aggacagacc ttaattgata gtttggacta ccagcctctt
     2401 cttgtaaaac caaacaatca tgaacttgga gcgatttttg gggttaaact cgaaagttta
     2461 gatgaaattg agaaatacgc tcgtgagtta ctggctaagg gtgctcaaaa tgttattatc
     2521 tctatggctg gtgatggtgc ccttcttgtc acatctgatg gagcttactt cgctaaacca
     2581 atcaagggaa cagtcaaaaa ttcagttgga gctggtgatt ctatggttgc tggattcaca
     2641 ggtgaatttg tcaaatcaaa agacgcagta gaagccttca aatggggagt ggcttgcgga
     2701 acggcaacta ccttctcaga tgacttggca acggcggaat ttattaaaga aacatatgga
     2761 aaagttgagg tagaaaaacg atgaaaattc aagacctatt gagaaaagat gtcatgttgc
     2821 tagatttgca ggcaactgaa aaaacagctg tcatcgacga gatgattaaa aatttgacag
     2881 accacggtta tgtaacagat tttgaaacat ttaaagaagg cattttggcg cgtgaagctt
     2941 tgacttctac tggtttgggt gatggaatcg caatgcctca cagcaaaaac gctgctgtca
     3001 aagaagcgac agttctattt gctaagtcaa ataagggtgt tgactacgag agcttggatg
     3061 gacaagcaac tgacctcttc ttcatgattg cagctccaga aggtgccaat gatactcact
     3121 tggcagcctt ggcagaattg tctcaatact tgatgaaaga cggttttgca gacaaacttc
     3181 gtcaagcaac atctgcagac caagttatcg aactttttga ccaagcttca gaaaaaactg
     3241 aggaacttgt tcaagcacct gctaatgact ctggtgactt tatcgtagct gttacagctt
     3301 gtacaacagg tattgcccac acttacatgg cccaagaagc ccttcaaaaa gtagctgctg
     3361 aaatgggggt tggtatcaag gtcgaaacca acggtgctag cggtgttgga aatcaactaa
     3421 ctgcagaaga tatccgtaag gctaaagcta ttatcattgc agcagacaag gccgttgaaa
     3481 tggatcgatt tgatggaaaa ccattgatca atcgtccagt tgctgacggt atccgtaaga
     3541 cagaagagct aattaacttg gctctttcag gagatactga agtctaccgt gccgctaatg
     3601 gtgccaaagc tgcaacagcc tctaacgaaa aacaaagcct tggtggtgcc ttgtacaaac
     3661 acttgatgag tggtgtatct caaatgttac cattcgttat cggtggtggt atcatgattg
     3721 cccttgcctt cttgattgac ggtgctttgg gtgttccaaa tgaaaacctt ggcaatcttg
     3781 gttcttacca tgagttagct tctatgttca tgaaaattgg tggagctgcc tttggtttga
     3841 tgcttccagt ctttgcgggt tatgttgcct actctattgc tgaaaaaccg ggtttggtag
     3901 caggtttcgt ggctggtgct attgccaaag aaggttttgc ctttggtaaa attccttatg
     3961 ccgcaggtgg tgaagcaact tcaactcttg caggtgtctc atctggtttc ctaggtgccc
     4021 ttgttggtgg atttatcgca ggtgccttgg ttcttgccat caagaaatac gttaaagttc
     4081 ctcgttcact cgaaggtgct aaatcaatcc ttctattgcc acttcttgga acaatcttga
     4141 caggatttgt tatgctagct gtgaatatcc caatggctgc aatcaacact gctatgaatg
     4201 acttcctagg cggtcttgga ggaggttcag ctgtccttct tggtatcgtc cttggtggaa
     4261 tgatggctgt tgacatgggt ggaccagtta ataaagcagc ttatgtcttt ggtacaggta
     4321 cgcttgcagc aactgtttct tcaggtggtt ctgtagccat ggcagcagtt atggctggag
     4381 gaatggtgcc accacttgca atctttgtcg caactcttct tttcaaagat aaatttacta
     4441 aggaagaacg taactctggt ttgacaaaca tcatcatggg cttgtcattt atcactgagg
     4501 gagcgattcc atttggtgcc gctgacccag ctcgtgcgat tccaagcttc atccttggtt
     4561 cagcagtagc aggtggactc gttggtctta ctggtatcaa actcatggcg ccacacggag
     4621 gaatcttcgt tatcgccctt acttcaaatg ctctccttta cctcgtttct gtcttggtag
     4681 gagcaatcgt aagtggtgtg gtttatggtt acctacgcaa accacaagca taaaaaatag
     4741 aaaaatgaaa agattggacc gtttggtgca gtctttttct cttcccgaaa tgcctgtgaa
     4801 atatggtata atagaagaat ggcaaacaag aatacaagta caacaagacg gagaccgtct
     4861 aaagcagaac tggaaagaaa agaagcgatt caacgaatgt tgatttcatt aggaattgcg
     4921 attttattga ttttcgcagt cttcaaatta ggggctgcag gtataaccct ttataattta
     4981 attcgcttgc tagtgggtag cctagcttat ctggcgatat tcggcctatt aatctatctc
     5041 ttctttttca agtggatacg aaaacaggaa ggactcttat ctggcttttt caccatattt
     5101 gctggcttac tcttgatttt tgaagcctac ttggtttgga aatatggttt ggacaagtcc
     5161 gttctaaaag ggaccatggc tcaggttgtg acagatctga ctggttttcg aacgactagc
     5221 tttgctggag ggggcttgat aggggtcgct ctttatattc caacagcctt tctcttttca
     5281 aatatcggaa cttactttat tggttctatc ttgattttag tgggttctct cctagtcagc
     5341 ccttggtctg tttacgatat tgctgaattt ttcagtagag gctttgccaa atggtgggaa
     5401 gggcacgagc gtcgaaaaga ggaacgcttt gtcaaacaag aagaaaaagc tcgccaaaag
     5461 gctgagaaag aggctagatt agaacaagaa gagactgaaa aagccttact cgatttgcct
     5521 cctgttgata tggaaacggg tgaaattctg acagaggaag ctgttcaaaa tcttccacct
     5581 attccagaag aaaagtgggt ggaaccagaa atcatcctgc ctcaagctga acttaaattc
     5641 cctgaacagg aagatgactc agatgacgaa gatgttcagg tcgatttttc agccaaagaa
     5701 gcccttgaat acaaacttcc aagcttacaa ctctttgcac cagataaacc aaaagatcag
     5761 tctaaagaga aggaaattgt cagagaaaat atcaaaatct tagaagcaac ctttgctagc
     5821 tttggtatta aggtaacagt tgaacgggcc gaaattgggc catcagtgac caagtatgaa
     5881 gtcaagccgg ctgttggtgt aagggtcaac cgcatttcca atctatcaga tgacctcgct
     5941 ctagccttgg ctgccaagga tgtccgaatt gaagcaccaa tccctgggaa atctctaatc
     6001 ggaattgaag tgcccaactc cgatattgcc actgtatctt tccgagaact atgggaacaa
     6061 tcgcaaacga aagcagaaaa tttcttggaa attcctttag ggaaggctgt taatggaacc
     6121 gcaagagctt ttgacctttc taaaatgccc cacttgctag ttgcaggttc aacgggttca
     6181 gggaagtcag tagcagttaa cggcattatt gctagcattc tcatgaaggc gagaccagat
     6241 caagttaaat ttatgatggt cgatcccaag atggttgagt tatctgttta caatgatatt
     6301 ccccacctct tgattccagt cgtgaccaat ccacgcaaag ccagcaaggc tctgcaaaag
     6361 gttgtggatg aaatggaaaa ccgttatgaa ctctttgcca aggtgggagt tcggaatatt
     6421 gcaggtttta atgccaaggt agaagagttc aattcccagt ctgagtacaa gcaaattccg
     6481 ctaccattca ttgtcgtgat tgtggatgag ttggctgacc tcatgatggt ggccagcaag
     6541 gaagtggaag atgctatcat ccgtcttggg cagaaggcgc gtgctgcagg tatccacatg
     6601 attcttgcaa ctcagcgccc atctgttgat gtcatctctg gtttgattaa ggccaatgtt
     6661 ccatctcgtg tagcatttgc ggtttcatca ggaacagact cccgtacgat tttggatgaa
     6721 aatggagcag aaaaacttct tggtcgagga gacatgctct ttaaaccgat tgatgaaaat
     6781 catccagttc gtctccaagg ctcctttatc tcggatgacg atgttgagcg cattgtgaac
     6841 ttcatcaaga ctcaggcaga tgcagactac gatgagagtt ttgatccagg tgaggtttct
     6901 gaaaatgaag gagaattttc ggatggagat gctggtggtg atccgctttt tgaagaagct
     6961 aagtctttgg ttatcgaaac acagaaagcc agtgcatcca tgattcagcg tcgtttgtca
     7021 gttggattta accgtgcgac ccgtctcatg gaagagcttg agatggcagg tgtcattggt
     7081 ccagctgaag gtaccaagcc aagaaaagta ttgcagcaat aaaaaatagc ttctttccaa
     7141 gtttggaagg aggctatttt agtgattatt gattagtttt attttccgaa gttggggtat
     7201 tggactgttt ttcgttttca gtagcaggtt tacttggagt aggagcagga gtagaagaat
     7261 cctgagttga tgttttctgc tcttcttttt tctcttcctt gacgctggat tttggcgttt
     7321 cttcttgttg tgttttttct tgagaagtct tattttcctt attgggacta gtattttcct
     7381 gaggggattc cttttgaatt tctttgacaa ttgttgtcgt ctggcttgtt gtaggttcct
     7441 ttttagtatt tttgttatta tccaaggcgt tcataatcgt gatactagcg gtaatcaagc
     7501 caaggatact agcgatagtc gcaacggttt tttgaaagac tgtaaagcct tttttgatgt
     7561 gttctgtttt tggttttgaa gttctacgat aattagacat acattctctc ctttaattaa
     7621 aatttattat acctcatttc tttaaaaaaa tcttaaaaaa agaggagatt tttctacacg
     7681 aacttcagta attagttgaa ataaaatatg acttatgata taacaaaata aagaaagcga
     7741 tttcaaaaaa ggagggcgtt atatgttcga aattagaaat ttgagtctct cttttgcagg
     7801 caggcggttg ctagagccaa cagatttgat atttgaaagg ggaaaagtat atacaatata
     7861 tgggaagtca ggcgtaggaa agtcttcttt actgaataaa attggtttgc tctctgctac
     7921 ggatcctagt gtttcttatt tcttcgatgg aaaagagatt gatatcgaaa ataagaaatc
     7981 agtctctgtc tttatagctg aagaagttgc ctttgtattt caggggcgaa atctaataaa
     8041 tgatttgacc gtatttgaaa atttaagact agctttaaac ttttatgact tgagtccaga
     8101 tgaaatagaa agcaaaattg atacagtatt ggcggattta cagatttctt ctttaaagca
     8161 tgcttatcca gaagatttat ccggtggtga agaacaacga gtggcaatcg caagagcctt
     8221 agttacagga aagactctca tcttagctga tgaaccaaca agttctttgg ataaagaaaa
     8281 tagagaaaaa atctccgcct tgttaattga tttagctcac aaatatcata aaattgtatt
     8341 gattgtttcg catgatgagg cgatgattgc aataggagat gttcagcttc attttaaaga
     8401 ccataaatta gttgggaatt gtcttatttt gaattctgat gaagggataa aggattctaa
     8461 gaagtggtcc tcctgtttgt cgtcttctca tacgaagcta tccgttttga aacatagaag
     8521 gcctttttta cctagattat tagcgttttt tatctctctt gtagttgcta tagcagtaac
     8581 ctctattaac tttcagtcct tatttgcaga taagtatcac caactggtga acaactcact
     8641 cgaaaatggc ttcttggtga ttaatgattc gcttcattta caaactacaa aagtgttgga
     8701 tgattttctc agttttgata aggaggaaat cacctcaatt agcacaagtc agaatattca
     8761 aaccgtcaat ccttatatag agtttctctc tttgggtatg acgtttgata attcaaaaga
     8821 ctattcacaa cttcaaaaga actttcagcc aactttgact attgatggtc agaccagaag
     8881 cttgacaaga aatttttcaa ttcaaccctt gtatcagaca aatacaacac agaggcagat
     8941 agagtatttt gataggagca atgaaaaagg gatttatcta tctgagcaat tcatttcaga
     9001 tgaaaaatta ccaaatatag tttcgggaac taccgtgaca ctgacttttt atattccaat
     9061 ttctttatat gaatcaagta tagagaaaga ggggaatata ttgaaaggag atggcggttt
     9121 atttgtcaaa gtggataaaa catatcctgt tttagggatt gttaaaaaaa gttatccgtt
     9181 tgaatattcc ccttatggga acaccctatt tatgaatatt gctgaaatgg aagaattgca
     9241 aaatgaagca attcaacagc atcccatgac gaagatggct ttagatggtt ttcctttgaa
     9301 atcatggatg ccgagtgcga ttcatgtgtt actaaaggat agcggtgcta ttcctgaaga
     9361 attgagtaga ataagagcaa tctcttcaca aattactatt ttatcatcat atgaaaacta
     9421 cgaagaattt aataaaggac tcacctatat caggcaattt ttaatgttgc tatccctagt
     9481 cttactcatc cttgtcatcg cagtattgtc ctttgttttc tttttgctca atcgtcctcg
     9541 acgctttgaa gtagggattt taaaatcttt gggatacagt actcaaaata ttgtttggct
     9601 tttcttaaaa gaattaattg gttatggtaa aacaatttcg atattagcaa gttgtttact
     9661 cgtcattctt tccattttag caatgcaagt gcttaaattg gaaatttcag atgtttttaa
     9721 attctatcta acttccgttt tcaccttaat tggattatca gtctcagtac tgataataag
     9781 tggcttacta cctatataca caacttgtag gcaaacagtt gtcgatacta tccgaaagaa
     9841 tggatgaggt gatatcatga aacataagat aatgaaaaaa ataatagtgg tagtgtttgc
     9901 tatcccatgg ttaacatttt taagtcaagt acatgcagat tatgtaagta agtctagttc
     9961 tttagacggt tatagcttgg taggcttgaa tgttcttaat gtgactttta atgcttctgg
    10021 ctattctgat acttatagtg gcagtgtgtc aaatatgtat ttcactgact gggcttggtt
    10081 tcctaatacg ataagtaatc gttccacttg gaaaagttcg attccatatg gtcaacgcgc
    10141 aaatggtaca gtccatatag gtgtaggagt aaattcgcct tggggagcgg ttaatctctg
    10201 gggcggacaa aattactttt ctttagattt ttaacagaaa taatcaaagt tagatagaat
    10261 gaataagaat gctcttgatg atggtatcaa gagtatttta tgttattcag attaatgcaa
    10321 acattgccct ttcttgtcgc aggctccgtt tcatgataca atagaaggag tgattttaga
    10381 aagcgtgaga aacatgattt actttgataa ttcggcgacg accaggccct atccagaagc
    10441 ccttgaaacc tatatgcagg tggcttcaaa aattttagga aatccatcta gtctccatcg
    10501 tttgggagac caggcaacac gaattttaga tgcttcccgt caacagattg cagatttaat
    10561 cggtaagaaa agtgatgaaa tcttctttac atctggtggg acagaagggg ataactggat
    10621 catcaaggga gtagcatttg aaaaagccca gtttggcaag cacatcatcg tatcagccat
    10681 tgaacatcca gcagttaagg aatcagccct ctggttgaaa gctcaaggtt ttgaagtgga
    10741 ttttgctcca gttgataaca aaggatttgt ggatgttgag gtgctagaag atttgataag
    10801 acctgatacg accctcgttt ccatcatggc agttaacaat gaaattggct ctgttcagcc
    10861 cattgaggct atttcaaaac tattggcaga caagccaact atttccttcc acgttgatgc
    10921 ggttcaggcg cttgccaaga ttccgactga aaagtatctg acagaacgag tggatttcgc
    10981 gactttctct ggtcacaagt tccacggaat ccgtggtgtt ggttttatct atatcaagtc
    11041 tggcaagaag attacgcctc ttttaacagg tggtggtcag gaacgtgatt accgttcgac
    11101 aactgaaaat gtggcaggaa ttgcagcgac cgctaaggct ctccgtttat ctatggaaaa
    11161 gctagatatc tttaggagca agactgggca gatgaaggca gtgattcgtc aagcacttct
    11221 ggactatccg gatatatttg tcttttcaga tgaggaagac ttcgcccctc atattctgac
    11281 ttttggaatc aagggtgttc gtggtgaggt cattgttcac gcctttgaag actatgatat
    11341 tttcatctca acgacctcgg cttgttcgtc caaggctggg aaaccagccg gaaccctgat
    11401 tgctatggga gtggacaagg ataaggctca gtcagctgtg cgtcttagtc tagaccttga
    11461 aaatgatatg agtcaggtcg agcagttctt gaccaagcta aaattaattt acaatcaaac
    11521 tagaaaagta agataggagc attcatgcag tattcagaaa ttatgattcg ctacggtgag
    11581 ctgtcaacca agggcaaaaa tcgtatgcgt ttcatcaata aacttcgcaa taatatttca
    11641 gacgttttgt ctatctatcc ccaagttaag gtaacagcag atcgcgaccg tgcccacgct
    11701 tacctcaatg gagcagatta cacagcagta gcagaatcgc tcaaacaagt atttggaatt
    11761 caaaactttt cccctgttta taaggttgaa aaatctgtag aagttctgaa gtctgctgtc
    11821 caagagatta tgcaggacat ctacaaggaa ggcatgacct ttaaaatctc tagcaagcgt
    11881 agcgaccaca cctttgaatt ggacagtcgt gaactcaacc aaacgcttgg aggggctgta
    11941 ttcgaagcca ttccaaacgt acaagctcaa atgaaaagtc ctgacatcaa tcttcaggtg
    12001 gagatacgtg aagaagcagc ctatctttct tatgaaaccg ttcgaggggc aggtggatta
    12061 ccagtgggaa cttctggtaa gggcatgctc atgttgtcag ggggaatcga ttcccctgta
    12121 gctggctatc tagcgcttaa acgaggggta gatatagagg cagttcactt tgccagccca
    12181 ccttacacga gtcctggtgc tcttaaaaaa gcccaagatt tgacccgtaa attaaccaaa
    12241 tttgggggaa atatccagtt tatcgaagtt ccttttacag agattcaaga ggaaatcaaa
    12301 gccaaagcgc cagaagccta ccttatgacc ttgacgcgtc gttttatgat gcgtattact
    12361 gaccgtattc gtgaggtacg aaatggttta gttatcatca atggggaaag tctaggtcaa
    12421 gtagccagcc aaacccttga aagtatgaag gctatcaatg ctgttaccaa cactcccatc
    12481 attcgtcctg tggttaccat ggacaagttg gaaatcattg acatcgccca ggaaatcgat
    12541 acctttgaca tttcaatcca accgtttgaa gactgttgta ccatttttgc accagatcgt
    12601 ccaaaaacaa atcctaaaat taagaatgcg gagcagtacg aagcgcgtat ggatgttgaa
    12661 ggcttggttg agcgagcagt ggctggaatc atgattactg aaatcacacc tcaagccgaa
    12721 aaagatgaag ttgatgactt gattgacaat ctgctctaat tcagaaaatc caaaagaata
    12781 gcgaaaatca gtaaaaaaag ttagtttttt ctctaaaaac aggtaaaaaa ctaactttt
//
LOCUS       DBERCA010000047        12648 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.047, whole genome shotgun sequence.
ACCESSION   DBERCA010000047 DBERCA010000000
VERSION     DBERCA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.047"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            19..1155
                     /gene="dnaN"
                     /locus_tag="ACL719_001555"
     CDS             19..1155
                     /gene="dnaN"
                     /locus_tag="ACL719_001555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGS0699492.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNITITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1220..1414
                     /locus_tag="ACL719_001556"
     CDS             1220..1414
                     /locus_tag="ACL719_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGS0699493.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1498..2613
                     /gene="ychF"
                     /locus_tag="ACL719_001557"
     CDS             1498..2613
                     /gene="ychF"
                     /locus_tag="ACL719_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGS0699494.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRASYHLLGLGTYFTAGEKEVRAWTFKRGMKATQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2684..3253
                     /gene="pth"
                     /locus_tag="ACL719_001558"
     CDS             2684..3253
                     /gene="pth"
                     /locus_tag="ACL719_001558"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGS0699495.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3254..6763
                     /gene="mfd"
                     /locus_tag="ACL719_001559"
     CDS             3254..6763
                     /gene="mfd"
                     /locus_tag="ACL719_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGS0699496.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKVPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASHPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSCLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIVENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6821..7087
                     /locus_tag="ACL719_001560"
     CDS             6821..7087
                     /locus_tag="ACL719_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGS0699497.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7080..7448
                     /locus_tag="ACL719_001561"
     CDS             7080..7448
                     /locus_tag="ACL719_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGS0699498.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7453..7575
                     /locus_tag="ACL719_001562"
     CDS             7453..7575
                     /locus_tag="ACL719_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGS0699499.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            7568..8836
                     /locus_tag="ACL719_001563"
     CDS             7568..8836
                     /locus_tag="ACL719_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGS0699500.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKISFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8833..10110
                     /gene="tilS"
                     /locus_tag="ACL719_001564"
     CDS             8833..10110
                     /gene="tilS"
                     /locus_tag="ACL719_001564"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGS0699501.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFKKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRNAILGISNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10114..10656
                     /gene="hpt"
                     /locus_tag="ACL719_001565"
     CDS             10114..10656
                     /gene="hpt"
                     /locus_tag="ACL719_001565"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGS0699502.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10672..12630
                     /gene="ftsH"
                     /locus_tag="ACL719_001566"
     CDS             10672..12630
                     /gene="ftsH"
                     /locus_tag="ACL719_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGS0699503.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         4357 a         1898 c         2578 g         3815 t
ORIGIN      
        1 ataaataaag gagaatccat gattcatttt tcaattaata aaaatttatt tctacaagca
       61 ttaaatacta ctaagagagc tattagttct aaaaatgcca ttcctatttt atcaacagta
      121 aaaattgacg tgaccaatga aggtattact ttaattggtt caaatggtca aatttcaatt
      181 gaaaatttta tttctcaaaa aaatgaagat gctggtttgt taattacttc tttaggttcg
      241 atccttcttg aagcttcttt ctttatcaat gtagtatcta gtttacctga tgtaactctt
      301 gattttaaag aaattgaaca aaatcaaatt gttttaacca gtggcaaatc agaaattacc
      361 ctaaaaggaa aagatagcga acaatatcca cgaatccaag aaatttcagc aagcactcct
      421 ttaatacttg aaacaaaatt actcaagaaa attattaatg aaacagcctt tgctgcaagt
      481 acacaagaga gtcgtccgat tttaacaggt gtccacttcg tattgagtca acacaaagag
      541 ttaaaaacag ttgcaacaga ctctcatcgc ctaagccaga aaaaattgac tcttgaaaaa
      601 aatagtgatg attttgatgt cgtaattcct agccgttctc tacgcgaatt ttcagcggta
      661 tttacagatg atatcgaaac tgtagagatt ttctttgcca ataaccaaat cctctttaga
      721 agcgaaaata ttagcttcta tactcgtctc ctagaaggaa actatcctga tacagatcgc
      781 ttgattccaa cagactttaa cattactatt acttttaatg tggtaaactt acgccagtca
      841 atggagcgtg cccgtctttt atcaagtgcg actcaaaatg gtactgtgaa acttgaaatt
      901 aaggatgggg ttgttagcgc ccatgttcac tctccagaag ttggtaaagt aaacgaagaa
      961 atcgatactg atcaggttac tggtgaagat ttgaccatta gtttcaaccc aacttacttg
     1021 attgattctc ttaaagcttt aaatagcgaa aaggtgacca ttagctttat ctcagctgtt
     1081 cgtccattta ctcttgtgcc agcagatact gacgaagact tcatgcagct aattacacca
     1141 gttcgtacaa attaagtgaa agaggttgag cctggctcgc ctcttttatg atataatcga
     1201 aaaagaaaag gagagtagta tgtatcaagt tggaaatttt gttgagatga aaaaatcaca
     1261 cgcttgtaca atcaagtcaa ctggtaaaaa ggctaatcgt tgggaaatta cacgtgtagg
     1321 agcagatatc aaaataaaat gcagtaattg tgagcatgtt gtcatgatgg ggcgatatga
     1381 ttttgagcga aaaatgaata aaattattga ctgagaaccc ttagttagag ggttagcact
     1441 ttatcccttt ttgtgttata atattaggga ttgaaatgaa aacggagaat gagaaatatg
     1501 gctttgacag caggtatcgt tggtttgcca aacgttggta aatcaacact atttaatgca
     1561 attacaaaag caggagcaga ggcagcaaac tacccatttg cgactattga tccaaatgtt
     1621 ggaatggtgg aagttccaga tgaacgccta caaaaactaa ctgaaatgat aactcctaaa
     1681 aagacagttc ccacaacatt tgaatttacg gatattgcag ggattgtaaa aggagcttca
     1741 aaaggagaag ggctagggaa taaattcttg gccaatattc gtgaagtaga tgcgattgtt
     1801 cacgtagttc gtgcttttga tgatgaaaat gtgatgcgcg agcaaggacg tgaagacgcc
     1861 tttgtagatc cacttgcaga tattgataca attaatctgg aattaattct tgctgactta
     1921 gaatcagtga acaaacgata tgcgcgtgta gaaaagatgg cacgtacgca aaaagataaa
     1981 gaatcagtag cagaattcaa tgttcttcaa aagattaaac cagtcctaga agacgggaaa
     2041 tcagctcgta ccattgaatt tacagatgag gaacaaaagg ttgtcaaagg tcttttcctt
     2101 ttgacgacta aaccagttct ttatgtagct aatgtggacg aggatgtggt ttcagaacct
     2161 gactctatcg actatgtcaa acaaattcgt gaatttgcag cgacagaaaa tgctgaagta
     2221 gtcgttattt ctgcgcgtgc tgaggaagaa atttctgaat tggatgatga agataaaaaa
     2281 gagtttcttg aagccattgg tttgacagaa tcaggtgtag ataagttgac gcgtgcatct
     2341 taccacttgc ttggattggg aacttacttc acagctggtg aaaaagaagt tcgcgcttgg
     2401 actttcaaac gtggtatgaa ggctactcaa gcagctggta ttatccactc agactttgaa
     2461 aaaggcttta ttcgtgcagt aaccatgtca tatgaagatc tagtgaaata cggatctgaa
     2521 aaggccgtaa aagaagctgg acgcttgcgt gaagaaggaa aagaatatat cgttcaagat
     2581 ggcgatatca tggaattccg ctttaatgtc taaaaattaa taaatggtgt caattaggtt
     2641 ggaaaaaaat tccaaccctt ttggcttttg aaaggaaaaa taaatgacca aattacttgt
     2701 aggcttggga aatccagggg ataaatattt tgaaacaaaa cacaatgttg gttttatgtt
     2761 gattgatcaa ctagcgaaga aacagaatgt cacttttaca cacgataaga tatttcaagc
     2821 tgacctagca tcctttttcc taaatggaga aaaaatttat ctggttaaac caacgacctt
     2881 tatgaatgaa agtggaaaag cagttcatgc tttattaact tactatggtt tggatattga
     2941 cgatttactt atcatttacg atgatcttga catggaagtt gggaaaattc gtttaagagc
     3001 aaaaggctca gcaggtggtc ataatggtat caagtctatt attcaacata taggaactca
     3061 ggtctttaac cgtgttaaga ttggaattgg aagacctaaa aatggtatgt cagttgttca
     3121 tcatgttttg agtaagtttg acagggatga ttatatcggt attttacagt ctgttgacaa
     3181 agttgacgat tctgtaaact actatttaca agagaaaaat tttgagaaaa caatgcagag
     3241 gtataacgga taaatggtga ccttattaga tttattctca gaaaatgatc agattaaaaa
     3301 atggcatcaa aatttaacag ataagaaaag acaactaata cttggtttat caacatctac
     3361 taaggcgctt gcaattgcaa gcagtttaga aaaagaagat aggattgtgt tattgacgtc
     3421 aacttatgga gaagcagaag gacttgttag tgatcttata tctatcttgg gtgaggaact
     3481 cgtctatcca tttttggtag atgatgctcc tatggtggag tttttgatgt cttcacagga
     3541 aaaaattatt tcacgggttg aagccttgcg ttttttgact gattcatcta agaaagggat
     3601 tttagtttgt aatatcgcag caagtcgatt gattttaccg tctcccaatg cattcaaaga
     3661 tagtattgta aaaatctcag ttggtgaaga atatgatcaa cacgcgttta tccatcagtt
     3721 aaaggaaaat ggctatcgaa aagttactca agtacaaact cagggcgaat ttagtcttcg
     3781 aggagatatt ttagatattt ttgaaatatc ccagttagaa ccttgtcgaa ttgagttttt
     3841 tggtgatgaa attgatggta tcaggtcatt tgaagtagaa acacaattat cgaaagaaaa
     3901 taagacagaa ctcactatct ttccagctag tgatatgctt ttgagagaaa aggattatca
     3961 acgaggacag tcagctttag aaaaacaaat ttcaaaaact ttatcaccta ttttgaaatc
     4021 atacctagaa gaaattcttt caagttttca tcaaaagcaa agtcatgcag attctcggaa
     4081 gtttttatct ttgtgctatg ataagacatg gactgtcttt gattatattg aaaaagatac
     4141 tccaatattc tttgatgatt atcaaaaatt gatgaatcag tatgaagtat ttgaaagaga
     4201 attagcgcag tactttacag aagaattaca gaatagtaaa gcattttctg atatgcagta
     4261 tttttctgat attgaacaaa tctataaaaa acaaagtcca gtgacctttt tctctaatct
     4321 tcaaaagggt ttaggaaatc tcaaatttga caaaatttat caattcaatc aatatcctat
     4381 gcaggaattt ttcaatcagt tttcttttct aaaagaagaa attgaacgat ataaaaaaat
     4441 ggattacacc attattctgc agtctagcaa ttcaatggga agtaaaacat tggaggatat
     4501 gttagaggaa tatcagatta aattggattc tagagataag acaagtatct gtaaagaatc
     4561 tgtaaactta atagagggta atctcagaca tggttttcat tttgtagatg aaaagatttt
     4621 attgataact gaacatgaga tttttcaaaa gaaattaaag cgtcgttttc gaagacaaca
     4681 tgtttcaaat gcagagagat taaaagatta caatgaactt gaaaaagggg actatgttgt
     4741 ccatcatatc catgggattg gtcaatatct aggaattgaa accattgaaa tcaagggaat
     4801 tcatcgcgat tatgtcagtg tccaatacca aaatggtgat caaatttcta ttcccgtgga
     4861 acagattcat ctactgtcca aatatatttc aagtgatggt aaagttccaa aactcaataa
     4921 attaaatgat ggtcatttta aaaaggccaa gcaaaaggtt aagaaccagg tagaggatat
     4981 agctgatgat ttaatcaaac tctactctga acgtagtcag ttgaagggtt ttgctttctc
     5041 agctgatgat gatgatcaag atgcctttga tgatgctttc ccttatgttg aaacggatga
     5101 tcaacttcgt agtattgagg aaatcaagag ggatatgcag gcttctcacc caatggatcg
     5161 acttttagtt ggggatgttg gttttggaaa gactgaagtt gctatgcgtg cagcctttaa
     5221 agcagtcaat gatcacaaac aggttgtcat tctagttccg acgacggttt tagcgcaaca
     5281 gcactatacg aattttaagg aacgattcca aaattttgca gttaatgttg atgtgttgag
     5341 tcgctttaga agtaaaaaag agcagactgc aacacttgaa aaattgaaaa acggtcaagt
     5401 cgatattttg attggaacac atcgtgtttt gtcaaaagat gttgtgtttg ctgatttggg
     5461 cttgatgatt attgatgagg aacagcgatt tggtgtcaag cataaggaaa ctttgaaaga
     5521 actgaagaaa caagtggatg tcctaacctt gaccgctacg ccaatccctc gtaccctcca
     5581 tatgtctatg ctgggaatca gagatttgtc tgttattgaa actccgccga ctaatcgcta
     5641 tcctgttcag acctatgttt tggaaaagaa tgatagtgtc attcgtgatg ctgtcttgcg
     5701 tgaaatggag cgtggaggtc aagtttacta tctttacaac aaagttgaca caattgttca
     5761 gaaggtttca gaattacagg agttgattcc ggaggcttcg attggatatg ttcatggtcg
     5821 aatgagtgaa gtccagttgg aaaatactct attagacttt attgagggac aatacgatat
     5881 cttggtgacg actactatta ttgagacagg ggtggacatt ccaaatgcta atactttatt
     5941 tattgaaaat gcggaccata tgggcttgtc aaccttatat cagttaagag gaagagtcgg
     6001 tcgtagtaat cgtattgctt atgcttatct catgtatcgt ccagaaaaat caatcagtga
     6061 agtctctgaa aagagattag aagcgattaa aggatttaca gaattgggct ctggctttaa
     6121 gattgcaatg cgagatcttt cgattcgtgg agcaggaaat cttttaggaa aatcccagtc
     6181 tggtttcatt gattctgttg gttttgaatt gtattcgcag ttattagagg aagctattgc
     6241 taaacgaaac ggtaatgcta acgctaacac aagaaccaaa gggaatgctg agttgatttt
     6301 gcaaattgat gcctatcttc ctgatactta tatttctgat caacgacata agattgaaat
     6361 ttacaagaaa attcgtcaaa ttgacaaccg tgtcaattat gaagagttac aagaggagtt
     6421 gatagaccgt tttggagaat acccagatgt agtagcctat ctgttagaga ttggtttggt
     6481 caaatcatgc ttggacaagg tctttgttca acgtgtggaa agaaaagata ataaaattac
     6541 aattcaattt gaaaaagtca ctcaacgact gtttttagct caagattatt ttaaagcttt
     6601 atccgtaacg aacttaaaag caggcatcgt tgagaataag ggattaatgg agcttgtatt
     6661 tgatgtccaa aataagaaag attatgaaat tttagaaggt ttgctgattt ttggagaaag
     6721 tttattagag ataaaagagt ctaaggaaga aaattccatt tgatattttt cttctataaa
     6781 atagataaaa atggtacaat aataaattga ggtaataagg atgagattag ataaatattt
     6841 aaaagtatcg cgaattatca agcgtcgtac agtcgcaaag gaagtagcag ataaaggtag
     6901 aatcaaggtt aatggaatct tggccaaaag ttcaacggac ttgaaagtta atgaccaagt
     6961 tgaaattcgc tttggcaata agttgctgct tgtaaaagta ctagagatga aagatagtac
     7021 aaaaaaagaa gatgcagcag gcatgtatga aattatcagt gaaacacggg tagaagaaaa
     7081 tgtctaaaaa tattgtacaa ttgaataatt cttttattca aaatgaatac caacgtcgtc
     7141 gctacctgat gaaagaacga caaaaacgga atcgttttat ggggggggta ttgattttga
     7201 ttatgctatt atttatcttg ccaactttta atttagcgca gagttatcag caattactcc
     7261 aaagacgtca gcaattagca gacttgcaaa ctcagtatca aactttgagt gatgaaaagg
     7321 ataaggaaac agcatttgct accaagttga aagatgaaga ttatgctgct aaatatacac
     7381 gagcgaagta ctattattct aagtcgaggg aaaaagttta tacgattcct gacttgcttc
     7441 aaaggtgata aaatggaaaa tttattagac gtaatagagc aatttttgag tttgtcagat
     7501 gaaaagctgg aagaattggc tgataaaaat caattattgc gtttacaaga agaaaaggaa
     7561 aggaagaatg cgtaaattct taattatttt gttgctacca agttttttga ccatttcaaa
     7621 agtcgttagc acagaaaaag aagtcgtcta tacttcgaaa gaaatttatt acctttcaca
     7681 atctgacttt ggtatttatt ttagagaaaa attaagttct cccatggttt atggagaggt
     7741 tcctgtttat gcgaatgaag atttagtagt ggaatctggg aaattgactc ccaaaataag
     7801 ttttcaaata accgagtggc gcttaaataa acaaggaatt ccagtattta agctatcaaa
     7861 tcatcaattt atagctgcgg acaaacgatt tttatatgat caatcagagg taactccaac
     7921 aataaaaaaa gtatggttag aatctgactt taaactgtac aatagtcctt atgatttaaa
     7981 agaagtgaaa tcatccttat cagcttattc gcaagtatca atcgacaaga ccatgtttgt
     8041 agaaggaaga gaatttctac atattgatca ggctggatgg gtagctaaag aatcaacttc
     8101 tgaagaagat aatcggatga gtaaagttca agaaatgtta tctgaaaaat atcagaaaga
     8161 ttctttctct atttatgtta agcaactgac tactggaaaa gaagctggta tcaatcaaga
     8221 tgaaaagatg tatgcagcca gcgttttgaa actctcctat ctctattata cgcaagaaaa
     8281 aataaatgag ggtctttatc agttagatac gactgtaaaa tacgtatctg cagtcaatga
     8341 ttttccaggt tcttataaac cagagggaag tggtagtctt cctaaaaaag aagataataa
     8401 agaatattct ttaaaggatt taattacgaa agtatcaaaa gaatctgata atgtagctca
     8461 taatctattg ggatattaca tttcaaacca atctgatgcc acattcaaat ccaagatgtc
     8521 tgccattatg ggagatgatt gggatccaaa agaaaaattg atttcttcta agatggccgg
     8581 gaagtttatg gaagctattt ataatcaaaa tggatttgtg ctagagtctt tgactaaaac
     8641 agattttgat agtcagcgaa ttgccaaagg tgtttctgtt aaagtagctc ataaaattgg
     8701 agatgcggat gaatttaagc atgatacggg tgttgtctat gcagattctc catttattct
     8761 ttctattttc actaagaatt ctgattatga tacgatttct aagatagcca aggatgttta
     8821 tgaggttcta aaatgaggga accagatttt ttaaatcatt ttctcaagaa gggatatttc
     8881 aaaaagcatg ctaaggcggt tctagctctt tctggtggat tagattccat gtttctattt
     8941 aaggtattgt ctacttatca aaaagagtta gagattgaat tgattctagc tcatgtgaat
     9001 cataagcaga gaattgaatc agattgggaa gaaaaggaat taaggaagtt ggctgctgaa
     9061 gcagagcttc ctatttatat cagcaatttt tcaggagaat tttcagaagc gcgtgcacga
     9121 aattttcgtt atgatttttt tcaagaggtc atgaaaaaga caggtgcgac agctttagtc
     9181 actgcccacc atgctgatga tcaggtggaa acgattttga tgcgcttgat tcgaggaact
     9241 cgcttgcgct atctatcagg aattaaggag aagcaagtag tcggagagat agaaatcatt
     9301 cgtcccttct tgcattttaa gaaaaaagac tttccatcaa tttttcactt tgaagataca
     9361 tcaaatcagg agaatcatta ttttcgaaat cgtattcgaa attcttactt accagaattg
     9421 gaaaaagaaa atcctcgatt taggaatgca atcttaggca ttagcaatga aattttagat
     9481 tatgatttgg caatagctga attatctaac aatattaatg tggaagattt acagcagtta
     9541 ttttcttatt ctgagtctac acaaagagtt ttacttcaaa cttatctgaa tcgttttcca
     9601 gatttgaatc ttacaaaagc tcagtttgct gaagttcagc agattttaaa atctaaaagc
     9661 cagtatcgtc atccgattaa aaatggctat gaattgataa aagagtacca acagtttcag
     9721 atttgtaaaa tcagtccgca ggctgatgaa gaggaagatg aacttgtgtt acactatcaa
     9781 aatcaggtag cttatcaagg atatttattt tcctttggac ttccattaga aggtgaatca
     9841 attcaacaaa tacctgtttc acgtgaaaca tccatacaca ttcgtcatcg aaaaacagga
     9901 gatgttttga ttcaaaatgg gcatagaaaa aaactcagac gtttatttat tgatttgaaa
     9961 atccctatgg aaaagagaaa ctctgctctt attattgagc aatttggtga aattgtctca
    10021 attttgggaa ttgcgaccaa taatttgagt aaaaaaacga aaaatgatat aatgaacact
    10081 gtactttata tagaaaaaat agataggtaa aaaatgttag aaaacgatat taaaaaagtc
    10141 ctcgtttcac acgatgaaat tacagaagca gctaaaaaac taggtgctca attaactaaa
    10201 gactatgcag gaaaaaatcc aatcttagtt gggattttaa aaggatctat tccttttatg
    10261 gctgaattgg tcaaacatat tgatacacat attgaaatgg acttcatgat ggtttctagc
    10321 taccatggtg gaacagcaag tagtggtgtt atcaatatta aacaagatgt gactcaagat
    10381 atcaaaggaa gacatgttct atttgtagaa gatatcattg atacaggtca aactttgaag
    10441 aatttgcgag atatgtttaa agaaagagaa gcagcttctg ttaaaattgc aaccttgttg
    10501 gataaaccag aaggacgtgt tgtagaaatt gaggcagact atacctgctt tactatccca
    10561 aatgagtttg tagtaggtta tggtttagac tacaaagaaa attatcgtaa tcttccttat
    10621 attggagtat tgaaagagga agtgtattca aattagaaag aataatcttt aatgaaaaaa
    10681 caaaataatg gtttaattaa aaatcctttt ctatggttat tatttatctt tttccttgtg
    10741 acaggattcc agtatttcta ttctgggaat aactcaggag gaagtcagca aatcaactat
    10801 actgagttgg tacaagaaat taccgatggt aatgtaaaag aattaactta ccaaccaaat
    10861 ggtagtgtta tcgaagtttc tggtgtctat aaaaatccta aaacaagtaa agaaggaaca
    10921 ggtattcagt ttttcacgcc atctgttact aaggtagaga aatttaccag cactattctt
    10981 cctgcagata ctaccgtatc agaattgcaa aaacttgcta ctgaccataa agcagaagta
    11041 actgttaagc atgaaagttc aagtggtata tggattaatc tactcgtatc cattgtgcca
    11101 tttggaattc tattcttctt cctattctct atgatgggaa atatgggagg aggcaatggc
    11161 cgtaatccaa tgagttttgg acgtagtaag gctaaagcag caaataaaga agatattaaa
    11221 gtaagatttt cagatgttgc tggagctgag gaagaaaaac aagaactagt tgaagttgtt
    11281 gagttcttaa aagatccaaa acgattcaca aaacttggag cccgtattcc agcaggtgtt
    11341 cttttggagg gacctccggg gacaggtaag actttgcttg ctaaggcagt cgctggagaa
    11401 gcaggtgttc cattctttag tatctcaggt tctgactttg tagaaatgtt tgtcggagtt
    11461 ggagctagtc gtgttcgctc tctttttgag gatgccaaaa aagcagcacc agctatcatc
    11521 tttatcgatg aaattgatgc tgttggacgt caacgtggag tcggtctcgg cggaggtaat
    11581 gacgaacgtg aacaaacctt gaaccaactt ttgattgaga tggatggttt tgagggaaat
    11641 gaagggatta tcgtcatcgc tgcgacaaac cgttcagatg tacttgatcc tgcccttttg
    11701 cgtccaggac gttttgatag aaaagtattg gttggccgtc ctgatgttaa aggtcgtgaa
    11761 gcaatcttga aagttcacgc taagaacaag cctttagcag aagatgttga tttgaaatta
    11821 gtggctcaac aaactccagg ctttgttggt gctgatttag agaatgtctt gaatgaagca
    11881 gctttagttg ctgctcgtcg caataaatcg ataattgatg cttcagatat tgatgaagca
    11941 gaagatagag ttattgctgg accttctaag aaagataaga cagtttcaca aaaagaacga
    12001 gaattggttg cttaccatga ggcaggacat accattgttg gtctagtctt gtcgaatgct
    12061 cgcgttgtcc ataaggttac aattgtacca cgcggccgtg caggcggata catgattgca
    12121 cttcctaaag aggatcaaat gcttctatct aaagaagata tgaaagagca attggctggc
    12181 ttaatgggtg gacgtgtagc tgaagaaatt atctttaatg tccaaactac aggagcttca
    12241 aacgactttg aacaagcgac acaaatggca cgtgcaatgg ttacagagta cggtatgagt
    12301 gaaaaacttg gcccagtaca atatgaagga aaccatgcta tgcttggtgc acagagtcct
    12361 caaaaatcaa tttcagaaca aacagcttat gaaattgatg aagaggttcg ttcattatta
    12421 aatgaggcac gaaataaagc tgctgaaatt attcagtcaa atcgtgaaac tcacaagtta
    12481 attgcagaag cattattgaa atacgaaaca ttggatagta cacaaattaa agctctttac
    12541 gaaacaggaa agatgcctga agcagtagaa gaggaatctc atgcactatc ctatgatgaa
    12601 gtaaagtcaa aaatgaatga cgaaaaataa ccctgagaga ggctggag
//
LOCUS       DBERCA010000048        12302 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.048, whole genome shotgun sequence.
ACCESSION   DBERCA010000048 DBERCA010000000
VERSION     DBERCA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.048"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(112..459)
                     /locus_tag="ACL719_001567"
     CDS             complement(112..459)
                     /locus_tag="ACL719_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0699504.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(570..2345)
                     /gene="spxB"
                     /locus_tag="ACL719_001568"
     CDS             complement(570..2345)
                     /gene="spxB"
                     /locus_tag="ACL719_001568"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGS0699505.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2552..4795)
                     /locus_tag="ACL719_001569"
     CDS             complement(2552..4795)
                     /locus_tag="ACL719_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS0699506.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTTIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFRLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4805..5176)
                     /locus_tag="ACL719_001570"
     CDS             complement(4805..5176)
                     /locus_tag="ACL719_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGS0699507.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5187..5582)
                     /locus_tag="ACL719_001571"
     CDS             complement(5187..5582)
                     /locus_tag="ACL719_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGS0699508.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     SRLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5766..5864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5948..6739
                     /gene="thiD"
                     /locus_tag="ACL719_001572"
     CDS             5948..6739
                     /gene="thiD"
                     /locus_tag="ACL719_001572"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS0699509.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDAYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLDSVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6949..7581)
                     /gene="thiE"
                     /locus_tag="ACL719_001573"
     CDS             complement(6949..7581)
                     /gene="thiE"
                     /locus_tag="ACL719_001573"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS0699510.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7574..8377)
                     /locus_tag="ACL719_001574"
     CDS             complement(7574..8377)
                     /locus_tag="ACL719_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS0699511.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="ACL719_001575"
     CDS             complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="ACL719_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGS0699512.1"
                     /translation="MRKHQLQVHKLTILSMMTALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="ACL719_001576"
     CDS             complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="ACL719_001576"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGS0699513.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9609..10259)
                     /locus_tag="ACL719_001577"
     CDS             complement(9609..10259)
                     /locus_tag="ACL719_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGS0699514.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10261..11646)
                     /locus_tag="ACL719_001578"
     CDS             complement(10261..11646)
                     /locus_tag="ACL719_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699515.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEKRNQGRSFLLISHRLDPLISVVDYWIEMTSQGLSHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11647..12207)
                     /locus_tag="ACL719_001579"
     CDS             complement(11647..12207)
                     /locus_tag="ACL719_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0699516.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNMLSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIRLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3680 a         2756 c         2370 g         3496 t
ORIGIN      
        1 atatgcaatt tctttatata aaaagaccgg attgctccga tctttcaata gttcatattc
       61 tcaatttcta ttttaaaaat agctaaggtt aacgtcaaat gactacgcga cctatttcat
      121 acgataaaaa tcaagcacta gaccagcagg tccttgaact aataaggact ctgttcccca
      181 atcggttaca gttggtccgt gtaaaacctt tataccaagc tcgttcaacc gtttgtagtt
      241 ctggtctaca tcctcaacct cgatatgaat aatgattcct gactgaaagt tttccaaagg
      301 aaccaaatga ttttgtgaca acataaggca gtgactacca atcgtaaact gagcaaaacc
      361 atcatcagca taatctgcct ttttatccaa aatatgctcc aagtcagcac agacttgggg
      421 aacatttgaa acgataatat ctaattgatt taaattcatt tactctcctc cataaaaaga
      481 ccggattgct ccgatctttt aaagttctgc tctatgaaaa tcaaagaata aagtctacaa
      541 gtttcatatt tgattttcgg cgagaggaat tatttaattg cgcgtgattg caatccttct
      601 tcttccaaga agagacggaa tggtacgagt tcttctgctt cgtatttttc cttgaaggct
      661 ttgatagctt cttctgagtg aagttttgga tccaattcaa gtacttctac tggaagtgga
      721 cggtgttgag tgatgcgagc atcgatgaca acagttttac cttctttgtt caatttaaca
      781 gcttctgcaa caactgcatc gatgtcttcg atacggtcaa ctgtgaatcc aacagctcct
      841 tgagcttccg caattttagc gtagtcagcg tttgtgaagt ctacaccaaa caagtgtttg
      901 tttgtatctt cgtatttgtt cttgatgaag ccgtactcag catttgagaa gacaaggttg
      961 ataactggaa ggtcgtattg aacgtttgtg ataacgtctg ggtagcacat gttgaatgct
     1021 ccatcaccca tgatgttcca tacttggcga tctggattgt ctttcttagc agcgatacca
     1081 ccaggaaggg caatacccat tgtcgcaaag agtggagatg tacgccacat gttcttaggt
     1141 gtcatgtgaa ggtgacgagt agatgtttga gtagtgttac ctacgtcgat tgagtagata
     1201 gcgtcttgat cagcatgttt gttgattgca ttgtaaactt gatacaattg caattcaccc
     1261 tcagttttac cttcgagttt gttcatgtaa tcacgccagt tttggttgtt cttaacgttt
     1321 gcacgccacc atggagttga ttcaactggg tttactttgt caaggatagc tttagctgct
     1381 tgaccagcat caccaaggat tgaagcgtca agggcatgac gtttaccaag tttgtaaggg
     1441 tcgatatcaa cttggatgaa tttttcagtg ttcttgaatg cttcgtaaac ttcagcaaat
     1501 gggaagtttg aaccaaggaa aagaactgtg tctgcttcaa agaccacttc gttggctggt
     1561 ttccaaccaa cacgataagc agaacctgtc aaaccttcat agttccattc gaaagcttca
     1621 aagtttttac cagttgtgat gattggtgct ttgattttac gtgacaattc agtaatcact
     1681 tcaccagctt taacaccacc aaatccagca tagataactg ggcgttcagc attgttcaag
     1741 atttcaacag ctttgtcgat ttcaacttcg ttcaaagcag gagcgatgaa tgagcgttcg
     1801 tatgaacctg aaccgtagta tgagttttcg tcgatttctt ggaaaccgaa gtttactgga
     1861 atttcaacaa cagctggacc ttttttagaa attgcagcac ggcaggcttc gtcaattact
     1921 tttggcaatt gctcagcgta agctacacgt ttgttgtaaa cagcgatacc gttgtacatt
     1981 gggttttggt taagctcttg gaaagcatcc atgttcaatt cgttaactgg acgtgatcca
     2041 aggatcgcta ggaatggagt gttatccata gctgcatcgt aaacaccgtt aatcaagtga
     2101 gtcgcacctg gaccacctga accaactgca accccgattg agccgccgaa tttagcttgc
     2161 ataaccgctg caagagcacc tgtctcttcg tggcgaactt gtaagaagcg gatatctttg
     2221 tcttcagcca aagcgtccat caatgagctg agtgttcctg atgggatacc gtagattgta
     2281 tctacgcccc atgttttcaa tacgttaagc attgctgcag atgcagtaat tttcccttga
     2341 gtcataatga taactctcct tcaatttttt tagatttgga gaatacgatt acatagaatt
     2401 ggaaacgttc tccaaatttt tactattcca ctgtatcata tttatgctga cttttctaaa
     2461 aatctgctca aaactctcta ttctctattc taatacagtt ttgaaagttc tgtcatttct
     2521 gttttataac aaagaaatct agtcattact tttagtctat tttactaaaa tttaacagaa
     2581 gggaactggt cagaacagat acagaactaa aggccatggc tagacctgcc aattctgggt
     2641 tgagagccag tccaacacct gaaaagactc ctgctgcaat cggaattccg acaacattgt
     2701 agataaaagc ccagaaaaga ttgagtagaa ttcgatgaaa ggttttctta ctcatatcaa
     2761 aggcacgaac cactcctaaa agattattgg ttgtcaacac caaatctgct gactcgatgg
     2821 cgatatctgt tccagctccc atagcaatcc ccacatctgc tacactaagg gcaggagcgt
     2881 cattgatacc gtccccaaca aaggctactt tccctgactg ttgcagttta tggatttcat
     2941 gggctttttc ttctggcaag acgcctgcaa tgacctcttc aattccgatt tgatctgcaa
     3001 tagcacgcgc cacaccagca ttgtctcctg tcagcatgac tgttcggaga ccacgttttt
     3061 ttagctgact gatggctagc ttagcatttt ccttaggaat atcttgcaaa gcaagcaagc
     3121 ctttgatttc attgtcaaca gctaagaaca caactgtctt agcttctttt tctagttctt
     3181 ctagtttatc ttgataagta ttagaaatat ccatgccatc cagcatttta gcatttccaa
     3241 gtaaaacttg ttttccattg attcgccctg aaacaccttt cccgtgcaag gtctgaaaat
     3301 tttcaacagt ttgaaactca agtccagctt cactcgctcg cttaacgata gcctcagcca
     3361 gtgggtgttg agaagcatct tccaaggagg ctgccaacct aaacacttct acttcgtcgc
     3421 cgatgacatc tgttaccaca ggtttccctt ccgtcaaagt cccggtctta tcaaagacaa
     3481 gggtttgaac tttctggatt tcctgtaaga cagttccatt tttgaggaga acccccatct
     3541 tggcactacg tcctgtcccc accataaggg ctgtcggtgt tgcaagtccc aaggcacaag
     3601 gacaggcgat aatcaaaacc gccactccgt agagaagaga ggacacaaag ctagctccaa
     3661 gcacaaccac actatccctg agcaagacga accaaaccca aaaggtcatg attcctaaaa
     3721 tgacaactac tgggacaaaa atccctgaaa tcttatccgt caagtcttga atcggcgcac
     3781 gacttgtctg agctttcttc acaaaatcca caatctgagc caaaacagtc tctgagccaa
     3841 ctttttctgc tctaaagaca agcgttccac tatgattgat ggttgagcca atgacagtat
     3901 ctccaactgt cttgtccaca ggcagactct cacctgtcac catggattcg tcaatactag
     3961 agacaccttc tactacgaca ccatcaacag caatcttttc accgggacgc actcgaatca
     4021 ggtcgcctac cttgacttgt tccaaaggaa cttggacata actatcatca ctcaagactt
     4081 ctgcggtttt agcttgcaag tccagtaatt tctccacagc ttgggacgta ttttttctca
     4141 ttttttcctc aaaaactgct cccaaaagaa cgaaaaagag gataaatcca gcactttcga
     4201 agtaaacagg gagaccagca aagagagcaa ctaggctata gaaataagcc actagagttc
     4261 ccagcgcaac caaggtatcc atgttggcat tgtgcttttt aaaactggcc caagcactct
     4321 ggatatatgg cttacctgca actaacataa tagtcgttgt tgctagaaag gttccccaat
     4381 gcatgacttg atgactaatg ctacctgtca acatcccaat catgagaatc acaagaggca
     4441 cagtaaagat actagtaatc caaaaacgtt gcaggagaga tagagatttt cgagtcttct
     4501 caacgactgt atagcttccc ttttgcatct tcatgccaca agaaaattca tgtcgcccta
     4561 attcttgagg tgtaaaacga atgactttct cctcatctac gccgattggt tccaagatac
     4621 cttcttcttc aaacagaatt tccttataac agtttgaagg agtagcacga tgaaaggtaa
     4681 tctcagctgg aattcccttt tgaagctgga tatgggctgg atgatagcct ttttcagctc
     4741 ggatacggat tttttgaatg ccattttcta agcttgcttt cacaatttct gtcatagtct
     4801 ccacctactc tacaatcatc ttgccgtgca tcatgttcat accacaagca aagccaaact
     4861 ctccagcctg ctcaggcgtg atttccacta catactcttc ccccattggc aggttcgtat
     4921 gtacaccaaa atctggaaaa acaatttgat ccagacatgg tgaaggatcc ttgcggtcaa
     4981 agacaatgcg tgctggcact gatttcttga ggacaatcaa ctcaggagta tagcctccca
     5041 tgacttccac tcgaatctct tggtatccat ttttttgctg ggctttttgt ccagattttt
     5101 caggcttttt gaaaaaccaa aacaagataa acgcgataag ggcaatacaa ataatggtta
     5161 caatactatt taacatgacg tctcctttac atacaattac atcttacttc tgttacagca
     5221 cttgatttct tctctgaaat cacagcttcc aagtcttcca agtcagtctg agtaaattca
     5281 cattctacaa tcaagtcagc caacaaattc ctaatcctac gggaacaaac cttgtctttg
     5341 atatcttgga caagtaaatc ccgactttgg tctagagtta aaagggctga ataaacaaag
     5401 gacttgcctt cttttttccg agtcaaacac tctttctcaa ccagccgaga caaaagtgtc
     5461 tgaaccgttg acttggacca atcaaaccgc tctgccaaaa ccctaatcaa atctgtactg
     5521 gtctgctccc cctgcatcca aataatcttc atgacctgcc attctgcatc tgaaatctgc
     5581 attaccatac ctccaaaatc tacatttgtc aattacactc atcagtatac tctcaaaatc
     5641 tacatttgtc aattatagaa ataatatttt cttcgaaaaa tagaatttta atcatttgaa
     5701 aaacgatttg cagtcaaata ttactatata aacaataaaa atatgctata ctaaagaaaa
     5761 aagaaaacaa ccactagggg tgcgtaaagc tgagattaac gactgttaga tccctctgac
     5821 tcaatctagg taatgctagc tgatggaagt ggaaatgata atggggacta gcagtcttct
     5881 attgcctttc taaaacagac tagcttgttc ttaagaatac aaacttcagt tggttgggag
     5941 gttttagatg acttatttac ccgttgcttt gaccattgca gggactgacc ctagtggtgg
     6001 tgctggcatt atggcagatt taaagtcatt ccaagcgaga gatgcctatg gaatggctgt
     6061 tgtaaccagt cttgtcgctc aaaataccag aggtgttcag ctaatcgaac acgtttctcc
     6121 tcaaatgttg aaagcccaat tggacagtgt cttttctgat attccacctc aggctgtaaa
     6181 aactggaatg ttggctacta ctgaaatcat ggaaatcatc caaccctatc ttaaaaaact
     6241 ggattgtccc tatgtccttg atcctgttat ggttgctaca agtggagatg ccttgattga
     6301 ctcaagtgct agagactatc tcaaaacaaa cttactacct ctagcaacta ttattacgcc
     6361 aaatcttcct gaagcagaag agattgttgg tttttcaatc catgaccccg aagacatgca
     6421 gcgtgctggt cgcctgattt taaaagaatt tggtcctcag tctgtggtta tcaaaggcgg
     6481 acatctcaaa ggtggtgcta aagatttcct ctttaccaag aatgaacaat ttgtctggga
     6541 aagcccacga attcaaacct gtcacaccca tggtactgga tgtacctttg ctgcagtgat
     6601 tactgctgaa ctagccaagg gcaagagtct ttaccaggca gttgataagg ccaaggcctt
     6661 tatcacaaaa gctattcaag atgcccctca actcggtcat ggttctggtc cagtcaacca
     6721 tacaactttt aaagattaag aaaaaaaact ctctagttcc cactttaagg gaattagaga
     6781 gtttttatac tcttcgaaaa tctcttcaaa ctacgtcagc ttccatctgc aacctcaaaa
     6841 cactgttttg agctgacttc gtcagtctta tctaaaacct caaggcagta ctttgagcaa
     6901 cctgcgacta gctttctagt ttactctttg attttcattg agtattaatt aggaaagaat
     6961 gtcatgcaac tttgttaaaa aggcttgcgt ttttgcctca atatcttctg cttgcatcaa
     7021 atcacgtaca acagctacac cagctatgcc agtgcccata agctgatcaa tattctccga
     7081 agtcaagcct ccaatagcaa ctactggaat ggcaaccgtt tggcaaattg ttttcaaggt
     7141 cgatatcaga gtaatgggcg cattttcctt ggtggtggtt gggaaaatgg ctcctgtacc
     7201 caagtaatct gcacctgatt tctccgcttc cagagctctt ttaaccgttt tagcggtgac
     7261 accgaggatt ttttcaggac ccaagacttg tcgggcaacc gaaactggga gttcgtcatc
     7321 tccaatatgc agaccagcag catcaaccgc aagacaaaca tccaaccgat catcgattat
     7381 caagggtacc tgataagcat ctgttatttc cttgacttgt tttgccagtt gataatattg
     7441 attggttgtg agattttttt ctcgcaattg gactatggta acccctgaac ggcaggccgt
     7501 ctcaactttt gcaagaaagc tttccacgga atcttgatag cgattggtta ccagatatag
     7561 tctaagtgct tctctattca taaacctctc ctttgatggt atctagccaa ttttcatctc
     7621 ttcttaggag cgaaagctga ttgagtactt ggtaacgaaa ttcttccaat cccattcctt
     7681 gaacaactat tttctcagca gcgatattga gataagagac tgctaagcaa gaagcttcaa
     7741 aaccagtctt tccttggctg agaaaaacgg ctgttaaggc tccaaccaaa tctcctgtcc
     7801 ctgttatcca gtctaattct gcacagccat ttcccagtac agcgacctga tttttcgaaa
     7861 cgacgaggtc cttgggacct gtgactaaga aagacatacc atgataggtc tgacaccagt
     7921 ctttcaagac ttgaagcaaa tcctccgttt cttgatcttt agcactcgca tcgaccccaa
     7981 cgccgtggtg ctttaatcca acaagacttc gaatttctga catgtttcct ttaaggaccg
     8041 taggtctata gtctaaaagg tctctgacta agctcttacg aatggatgaa gctgttacgc
     8101 caaccgcatc tactaccatt gggagacaaa cttgagctgc ataagaagct gctatacgga
     8161 ttgctttctc cttctcagct gacaaatgcc ccaaattgat gaagagagcc tgactttgct
     8221 tagtaaaatc aagaacctca cggggatcat ctgccatgac aggtttgcat cccagagcca
     8281 aaatcccatt tgccagcatc tcacaagaaa tctcattggt catacagtga atgagggaac
     8341 tagagcctat aggaaaggga tttgtaaatt cctgcatcag tctatccttt cactaaagaa
     8401 atatccctgc acttttttaa agaattcctg cttgattaaa aatcgaaagg caataaagga
     8461 aatcgctgta ccaatcaagg ttgctccgaa aaatcgaggc gtgtagataa accagctaag
     8521 cttagcagct gatcctgtaa agagtaccat aacaggatag gaaacaatag aaccaataat
     8581 acctgttccc acaatttctc ccaaggcaga aaagtaaaat tttcgaccgt acttataaaa
     8641 gagacctgct agaagggctc caaaagtcgc tcctgtgaga gctaaaggcg gaatcccttg
     8701 agtcgtcata cggataaagg ctgtcactgt agccatggcc aaggcataaa caggtcccat
     8761 catgattccc gctagaatat tgactacact ggacatcggt gccattccct caatccgaaa
     8821 gataggtgta aggactacat caagggcagt catcatagat aaaatggtca atttgtgaac
     8881 ttgtagttgg tgctttctca tgtttctatt cttctccttt ttctaaagac tgtaaatcgc
     8941 tcttccatgt ctggtgttgg taagccatct cccaaaactt ggcttccata tgaacactga
     9001 tgtggaaggc atctagcatt ttttgcttat ctgtctcatc actttctcga tagagctgat
     9061 tgaccagtgc tccctcctct ctgatctgtt gctctaactc atccgtaata taagtttcaa
     9121 tccattgttg atagagagga tttggtgatg gtttaagatt aagtgatttg cctatatcat
     9181 ggtataacca aggacaagga agcaagcttg caaaagcgat ggctaagttc ggttctgcaa
     9241 attgcctata aatatgagaa atgtaatgat aacaggttgg agcgattgga tgttgctcca
     9301 tttcctggtc gttgatttcc aattccttga aaaattgttg gcgaataaat aactcaccct
     9361 ccactaaacc ctgagcattt tgtttcaaga gtcttttcat ctcttggttt gaagtcttat
     9421 cagccaaaag atgatagatt tctgagaagg ccttcagata gtaggcatcc tgaatcaggt
     9481 aatagcggaa aatggcaggt tctaaattcc cctcttgtaa ttgtaaaata aagggatgat
     9541 gaaaggaagc ctgccaagct ttcttggata attccatcgc aatatctgta aattccataa
     9601 taactccttt ataaaaatag actggtttga agcaataaaa agaaaagcag gtagattaat
     9661 tttgtttttt taggaatata aaaagtccga tagctattct tcaactgtgc atgttcgtca
     9721 tatccatgag cagacagagc tctcaggtaa agatggcgcc acctaaagac tgtcatcaga
     9781 accttactgt aaatcaaggg cgaccaaaaa tgcagttctt gaccacgtaa taggcaagct
     9841 tctttgaggg acttgatttc ttgctgaatg agaggaaagg aattgaatac cacaatcaag
     9901 gcataggacc aagagcgtga tagccccttt tgagccaagt acaagagaag ctcttttagt
     9961 gaaatagagg aaacaaagac aaggccgata caaactgtca caaaggccct cgttccaagc
    10021 atgactgcct gtgaagcatc tccgtgtaac tgaactgccc agtagttggc aaaagatggt
    10081 aaaatggcaa gtatgatcat ccaagctaac attttaaatc gacggtaata gagcataaag
    10141 agaatacaaa atgcgactac cgaaagattc agagcaatcg aaggaatgaa agatgtttcc
    10201 aaggataaaa tcagcaagaa gagactgata atcggtgtct gggttgctac tttgaccata
    10261 ctatctcacc tccccttggg tattgctact ctgagatgta agtggtttgg taatggtcac
    10321 ttctttcaca tgactgagcc cctgactagt catctcaatc caataatcaa ccacagaaat
    10381 caaagggtct aaacgatgac taatgagcag aaaacttctt ccttgattcc tcttctccac
    10441 aatccacttg caaaaataat ggcaggctct atcatccaaa cctgcaaaag gttcatctag
    10501 caagatcacg gaagccttac tggtcaagat ggtcaggagc tgaagaattt tttgctgacc
    10561 accacttaat tgatagggac tcttatcgac tgcctgctcc aaatcaaaat atcgtaaagc
    10621 ttgaaaaatc cgctgatttc tttcagaatc aggtccatct aattgaagct cctctcgcag
    10681 acttactcgg ataaactgct tctcagcttc ctgaacaaca cccgtcagat cacggtacaa
    10741 actctttttc tttttcagga ctgtcccctt ccatgcaatg cgccccttat acttttgaaa
    10801 ttgaagaata gagcgaaaga gggttgattt cccgacacca ttgtcaccca ggatacagga
    10861 aatcccttga tagaatgtga aatcagcaat tgaaaagagg gggcgattac caagctcacc
    10921 agtcacacgg ttcatatgga atagttccgg gctagaagca acttcctttg aagcaacctg
    10981 tgtcatctca taggaaggga tttgaaacac ttcccttagt tttccgtctc ttagctcaac
    11041 catatggtcg atataggctt tatagtcaga taaatcatgg tcgcacaaaa taactgtctt
    11101 cccatcaaga gccaactctt ttagaatctc caatatctcg attctgctct tgcggtcaat
    11161 agaagcgaag ggctcatcca agagatagac cctaggattc atggcaaaga ggacagccag
    11221 cgctgctttt tgcttttccc cacctgatag gtgatgaatg ggacggtgta agattgcctc
    11281 gcaacgacat tgctggacaa cctctgctat tttagaatca atttcctgaa ggtgatagcc
    11341 gatattctcc aaggtgaaaa tcagctcctc aaacaagttc tccatggtaa attgatgatt
    11401 gggattttga aagagaatgc caaccttctg gacacgtgcg atgatagaaa gctgactgac
    11461 ctcgctccca tctatcagga cttgaccact atagggaaga gaactgactt gggcaatgac
    11521 ttgaaaaagg ctggattttc ctgaaccact actcccaact aacaaggtaa aggcttgcgc
    11581 atgaaaagta aaatcaaacg gctcagagaa gattggggac tgaatcgctc gtagttccag
    11641 acccatctat gcttttcctc cagttgcaaa ctgatgatag agtttgacaa tggcacgaac
    11701 caagatggta cagaagaaat aaacagaaat aaaacgtacc acaagtaagg aaaggacaaa
    11761 cggaagggaa aaggcgtagt aacctaacct aatgtattca tagacaaagc taacaagcgt
    11821 aatcccaata ctattagcag ttagagagag ccaactttca tagcgattct tagttacgat
    11881 aaaaccaaat tcacttccca aaccttgaac caagccagac aaaagagctc ctagaccaaa
    11941 ttggctacca taaaggactt cagcaagcgc agctagcact tctccaatcg ttgcacttcc
    12001 gactctcgga acaaagatgg cagcaatggg cgcagccata caccagagac cgaagaggat
    12061 ttcattggca aaggcctgca aaccaagagg tgttaagagt agactgagca tattatacac
    12121 atatcctgaa ccaacgaaaa ccccaccaaa aaagatagac aagaaagcaa gcaagataac
    12181 atcttttaac tgccattttt tcaacataaa aaactccttt tttaaaagaa aagtggggca
    12241 ctcaagaaga ccgacctaaa tactttgtat agcagattga atttagaaca gtacacagga
    12301 at
//
LOCUS       DBERCA010000049        12191 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.049, whole genome shotgun sequence.
ACCESSION   DBERCA010000049 DBERCA010000000
VERSION     DBERCA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.049"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1742
                     /locus_tag="ACL719_001580"
     CDS             <1..1742
                     /locus_tag="ACL719_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS0699517.1"
                     /translation="GTESLFKALEGYRKVFLPTTSNNEWFKAQTKAYIVEEKSSIPEV
                     SKKQSEQGTKYSIGIYDRLTSPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDR
                     YRSKVHPKGDNLNKFVEDNVREAAKRFRDHYDYWYKILEPENREKLYRSLLVYDAFKF
                     GRDNTEDKVTYQADFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKD
                     GAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYD
                     DSENSTRLQVADPTQRFNSAEDLHNYMHNMFDLIYTLEILEGRAVAKLDYNAKNDLLR
                     KIENKYKQDPDGNSVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRGYKDQEYKRNG
                     YYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQYEKDAKAA
                     GSKIKSYGKEVGLVTDKLVLEKVFNNRYSSWVEFKKAMYNERIAKFNNLISVSFNNPN
                     VSWGRTNRITITSIANLQNMINTAVNEDAEDFRAQLYPDTNSRVLKLKKAIYKAYLDQ
                     TNDFRRSIFENKK"
     gene            1786..7023
                     /gene="zmpD"
                     /locus_tag="ACL719_001581"
     CDS             1786..7023
                     /gene="zmpD"
                     /locus_tag="ACL719_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpD"
                     /protein_id="HGS0699518.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSIFLGSFLFALSVVGVSTYHYLDYS
                     TLTQTERDQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTVLGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDR
                     TVSSVSPASTVSPVEQPKVVTDKGEPEVQPALPEAVVTEKGEPEVQPALPEAVVTEKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDNSVKQVSEDGERQIVT
                     SYEELHGKKISESVETVTILKEMKPEIIVKGTKEKTAPVLTLTKVTEDAMNRSANLNY
                     ELDNKDNAEISSIVAEIKDGDTVVKRVDLSKEKLTDAVQNLDWFKDYKIATTMIYDRG
                     QGSETSKLDERPLRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMY
                     LKITSRDNKVTRLTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKP
                     HPKEDNVYYNFKDLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEG
                     KHYTIHNTERPLFQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVT
                     GNVLGRNWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYV
                     DANLIGKKAKAAGIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLS
                     HHGYIGEVVSMMKVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKE
                     HHRIKPISQSEADTKIMMLGITAQDFAINEPVVNRLNRLTRKEDEYKSTQDYKVDRDL
                     AYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMV
                     HYADGTKEEMDVTKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASV
                     QLISPEVRALMDARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADH
                     QLNSDETAMKALVKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSV
                     DLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDW
                     FIHAAKNVYVSEPQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYN
                     TMAYSSFEKYGKYTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRV
                     PTPVWDNQNYSGIKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNP
                     NNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTP
                     SSIGHQGEYGALGLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHV
                     EADAVLPQLNGDNSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSS
                     INDLIDNNFMTKHGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKH
                     NAFRMWGYYGYDKGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKY
                     WYHDVKSRAEKGFTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVF
                     KALLKNTDGFFNPLFKKDI"
     gene            7190..8536
                     /locus_tag="ACL719_001582"
     CDS             7190..8536
                     /locus_tag="ACL719_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGS0699519.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFRLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSCLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKMDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
     gene            8951..9802
                     /gene="ylqF"
                     /locus_tag="ACL719_001583"
     CDS             8951..9802
                     /gene="ylqF"
                     /locus_tag="ACL719_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGS0699520.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEPSVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            9789..10568
                     /locus_tag="ACL719_001584"
     CDS             9789..10568
                     /locus_tag="ACL719_001584"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGS0699521.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            10584..12134
                     /locus_tag="ACL719_001585"
     CDS             10584..12134
                     /locus_tag="ACL719_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGS0699522.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLRLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         4098 a         2084 c         2476 g         3533 t
ORIGIN      
        1 atggaactga aagtttattt aaagcattag aaggatatag aaaagtattc ttaccaacaa
       61 cttctaacaa tgagtggttt aaagctcaaa ctaaagcata tattgttgaa gaaaaatctt
      121 ctattccaga agtaagtaaa aaacaatcag aacaaggaac taagtattct ataggaattt
      181 atgatagatt aactagtccg tcatggaaat atcaaagtat ggtcttacca ctattaactt
      241 tacctgaaga aaaaacagta tttatgatag cgaatatttc aacaatagga tttggtgctt
      301 atgatagata tagaagtaaa gtccatccta aaggagataa cttaaataaa tttgtagaag
      361 acaatgtaag agaagctgca aaaagattta gagatcatta tgattattgg tataaaatat
      421 tagagcctga gaatagagag aaactttata gaagtttatt agtatacgat gcatttaaat
      481 ttggtcgaga taatactgaa gataaagtaa cttatcaagc tgattttgaa actgatcatc
      541 cagctataaa atacttcttt ggacctgcgg ggaataatgt tgttcataat ggacacggag
      601 catatgctac cggagatgca ttctattata tggcatatcg tatgttggat aaagatggag
      661 ctgtaacata tactcatgaa atgacacata actctgatag agaaatttat cttggaggat
      721 atggaagaag aagcggatta gggcctgagt tttatgctaa gggcttatta caagcacctg
      781 accacccata tgatccaaca ataacaatta actctgtgtt gaaatatgat gattcagaaa
      841 attcaacaag acttcaagta gctgatccaa ctcaacgatt taatagtgca gaagacctac
      901 ataattatat gcataatatg tttgatttaa tatatactct tgaaatatta gaaggtcgtg
      961 ctgtagctaa attagattat aatgcgaaaa atgatttatt aagaaaaatt gagaataaat
     1021 ataagcaaga tccagatggt aattctgttt atgcgacaaa tgttgttcgc agattaacga
     1081 tggatgaagt taataaatta aattcttttg atagtttaat tgaaaatgat attattacta
     1141 gcagagggta caaagaccaa gaatataaga gaaatggtta ttatacaatt gatttatttt
     1201 caccgattta ttcagctctt agtggagaga aagggactcc tggtgattta atgggacgtc
     1261 gtatagcatt cgaattatta gctgcaaaag gatataaaga gggcatggta ccttatatct
     1321 caaatcaata tgaaaaagat gcaaaagctg cgggaagtaa gattaaatct tatggtaaag
     1381 aagtaggttt agttacagat aaattagtac ttgaaaaggt atttaataat cgttactcat
     1441 cttgggtaga atttaaaaaa gcaatgtata atgagcgaat agctaaattt aataatctaa
     1501 taagtgttag ctttaataat ccaaatgtaa gttggggaag aactaataga ataacaataa
     1561 ctagcatagc caaccttcaa aatatgataa atacggctgt caatgaagat gctgaagatt
     1621 ttagagcaca attataccct gatacaaata gtagagttct taaactgaaa aaagcaatat
     1681 ataaagccta tcttgaccaa acaaatgatt ttagaagatc aatttttgag aataaaaaat
     1741 agtgtttact attaggaaat aaaaagtgaa tgtggaggat tagaattgtc attattaaaa
     1801 aaagataaat tttccattag gaaaataaag ggtattgttg gttcgatatt cttggggagt
     1861 tttttatttg cactgtcagt tgttggtgta tcgacttatc attacttaga ttatagtact
     1921 ttgacacaaa ctgaacgtga tcaactcaaa caaggtagac ctgatgaatc aaaagagtca
     1981 tatgctttgg tttatgaaaa agacgcatta ccaaacacag gaagttctca atctattatg
     2041 actgtattgg gtttgttagc tatcgggagt cttattgtga ttatcacaaa agataaaaag
     2101 aggaaaaaaa tagctacatt tttgattgta ggggcgacag gtttagttac actatcaact
     2161 gcttcagcac ttaatttgaa tgctaacatc catgagtctg gacgtgatgg tgtgttgcaa
     2221 atttctggtt acagatatgt tggttacttg gagcttgatg atagaactgt ttcgtcggtt
     2281 tctccagcat caactgtttc tccagttgag caacccaaag ttgtgaccga caaaggcgaa
     2341 ccagaagttc agccagcgtt accagaagca gttgtaactg aaaaagggga accagaagta
     2401 caaccagcct tacccgaagc agttgtaact gaaaaagggg aaccagaagt tcatgagaag
     2461 cctgactata cgcaaccaat aggtgcaaac cttgttgagc ctgaagttca tgagaaacta
     2521 gcatacactg aacctgtagg tacaacagga gtagatgaaa atggaaactt gattgagcca
     2581 cctgttaacg atattccaga gtatactgaa ccaatatcta cagtttcaga agttgcatca
     2641 gaaagagaag agttgccttc tttacatacc gatattcgta cagagactat tcctaaaacc
     2701 actatagaag aatctgatcc tagcaaattt ataggcgata attctgttaa acaagttagt
     2761 gaggatggcg aacgtcaaat tgttactagc tatgaagagt tacatggcaa aaaaatcagc
     2821 gagtcagttg aaacagtaac aattttgaaa gaaatgaagc ctgaaattat tgtaaaaggt
     2881 acaaaagaaa aaacggctcc tgttttaacc ttgaccaaag taacagaaga tgctatgaat
     2941 cgttctgcca acttaaatta tgagcttgat aataaagata atgctgaaat atcaagtatt
     3001 gtagctgaaa ttaaagatgg tgatacagta gttaaaaggg ttgatttatc taaagaaaaa
     3061 ttaactgacg ctgtacaaaa tttggattgg tttaaagatt ataagattgc aacaacaatg
     3121 atttatgatc gtggtcaagg ttctgaaacg tctaaactgg atgaaagacc tctgcgtttg
     3181 gagttgaaaa aggtcgaaat taaaaatatc gcatctacta atttagtgaa ggtaaatgat
     3241 gatggtactg aaacacctag tgattttatg aatgaaaaac cttcagaaga agatgttaaa
     3301 aaaatgtact taaaaatcac tagtcgcgat aataaggtaa ctcgtttaac tgtagactct
     3361 attgaagaag tgactgaaga aggtcaaaaa ctttacaaaa taactgctga agcacaagat
     3421 ttgattcaac acacagaccc aacgaaggtt cgtaataagt atgtttatta tatagaaaaa
     3481 ccccatccta aagaagataa tgtctactat aactttaaag atttagtaga tgctatgaat
     3541 actgataaaa atggtacatt taaacttggt gcagatttga atgccactgg tgttccaaca
     3601 cctaagaaat ggtatgttga cggcgacttt agaggtacat tgaagagtgt agaggggaaa
     3661 cattatacta tccacaacac ggagcgtccg ttattccaga atattattgg tggtacagta
     3721 accaaagtaa atctgggaaa tgtaaatatc aatatgccat gggcagaccg catcgcaccg
     3781 attgctgaca ctattaaagg tggcgctaaa attgaagatg tcaaagtgac tggtaacgta
     3841 ttaggtagaa actgggtatc tggatttatt gataagattg acaaccaagg tacactcaga
     3901 aatgtagcct ttatcggaaa tgtgacatct gtaggtgatg gtggtcaatt cttgaccggt
     3961 atcgttggtg agaactggaa aggactagtc gaacgtgcgt atgttgacgc aaatcttatt
     4021 ggcaagaaag ccaaggctgc tggtattgct tattggacac aaaacagtgg tgacaatcat
     4081 aaagttggtg tcgaaggtgc agtcaagaaa ggaattgtga agggtaccat tcaggtagaa
     4141 agtcctgttg aagtcggcgg tgctgttgga cgtttgagtc atcatggtta tatcggggaa
     4201 gtcgtatcta tgatgaaagt taagaagggt gaaatttttt atggttctag tgatatgaat
     4261 gatgacccat attgggtggc gaataatgta cgaggaaatt atgtcgttaa tggtgtgagt
     4321 gaaggtacga tatcttatgc tcgtgctaaa gaacaccatc gtatcaaacc aatttcacag
     4381 tcagaagcag atactaaaat tatgatgtta ggtattacag cacaagattt tgctatcaac
     4441 gaacctgtgg tcaatcgctt aaaccgttta accagaaaag aagatgaata taagtcaacc
     4501 caagactata aagtagatcg agatctggct tatcgtaata ttgagaaact tcaaccattt
     4561 tataacaaag aatggattgt caaccaaggt aacaaacttg cagaagattc aaacttagct
     4621 aagaaagaag tcctttctgt tacgggtatg aaggatggac agtttgtaac tgacttatca
     4681 gatattgata agattatggt tcactatgct gatggtacta aagaagaaat ggacgtcact
     4741 aagaatgctg attctaaagt gaaacaagtt cgtgaatata ccatcgctgg acaaaacgtt
     4801 gtttatacac caaatatggt tgagaaagac cgtaaccaat tgattcaaga tatcaaagat
     4861 aagttggcgt cagttcaatt gatttctcct gaagtacgtg ctttgatgga tgcacgtaag
     4921 aaaccagaag aaaatactga tgaacgtaag aatggttata ttaaagacct ttacttagaa
     4981 gaaagctttg ctgaaacgaa agctaatctt gataaattgg ttaaatcctt agttgaaaat
     5041 gcagatcatc aattaaatag tgacgaaacg gctatgaaag cacttgttaa gaaggtggat
     5101 gaaaataaag ctaaaatcat gatggctctg acttatctca atcgttacta tgatatcaag
     5161 tatggggata tgactatcaa gaacttgatg atgtttaaac ctgatttcta tggtaaatca
     5221 gttgaccttc ttgatttctt gattcgaatt ggatcaagtg agagaaatat caaaggtgac
     5281 agaactttgg atgcttatcg tgatatgatt ggtggaacta ttggtaagtc tgaattacat
     5341 ggattcttag attacaatat gcgtctcttc actaacgata ctgatttaaa tgattggttc
     5401 atccatgcag ctaagaatgt atatgtatca gaacctcaaa caacgaaccc agattttgtt
     5461 aataaacgac atcgtgcatt tgatggtttg aacaacggtg ttcataatcg tatgattcta
     5521 cctctattaa ctcttaagaa tgcacatatg ttcttgattt caacttacaa tacaatggct
     5581 tatagttctt ttgagaaata tggtaagtat acagaagaag ctagaaatga attcaaaaaa
     5641 gaaattgata aggttgcaca tgctcaacaa acatacttag acttctggtc acgtttagca
     5701 ttgccaagcg tacgtgacca attactcaag agtgagaata gggtaccaac tcctgtgtgg
     5761 gataaccaaa actatagtgg tattaaagga ataaaccgta tgggttatga tgagaagaaa
     5821 gtaccaattg cgccaattcg tgagttatat ggtccaactt ggaaattcca taataccaac
     5881 tggaatatgg gagctatggc atctattttc ccaaatccta ataacaatga ccaagtttat
     5941 ttcatgggaa caaatatgat tagtccgttt ggtatttcag cctttactca tgaaactaca
     6001 cacgttaatg atagaatgct ttacttcggt ggtcatagac accgtcaagg tacagacgtc
     6061 gaagcctatg ctcaaggtat gctacaaact ccgtcaagta ttggtcacca aggtgaatat
     6121 ggtgctttag gtttgaacat ggcttatcat cgtgaaaatg atggtgacca atggtataac
     6181 tatgaccctg ataagcttca aactcgtgaa gatatcgatc gatatatgaa aaactataat
     6241 gaagcactga tgatgctaga tcatgtagaa gcagacgccg tacttccaca attgaatggt
     6301 gataatagca agtggttcaa gaaaattgat agagaaatgc gcagaaatct tggggatggt
     6361 ttgaataact tagtagcacc gcaccaatgg gacaacgttc gtgacttaaa tcaagaagag
     6421 agttctaaga aattatcaag tattaacgat ttgattgata ataatttcat gaccaaacac
     6481 ggaaatccag gtaacggacg ttatagacca gaggatttta gaccaaactc agcttacgta
     6541 aatgtcaata tgatggcagg aatttacggt gggaatacga gtcaaggtgc tcctggttca
     6601 ttatcgttca aacataatgc cttccgtatg tggggttatt atggatatga caaaggtttc
     6661 actagttatg tatctagcaa atatcaaggt gaagctgata aacaaaacca gggtagatta
     6721 ggtgatgatt tcattatcaa aaaagtttca aacaaccaat tctcaaatct tgaagattgg
     6781 aagaaatatt ggtatcatga tgtgaaatcc agagctgaaa aaggattcac agagattaca
     6841 attgatggac aaaccattca caactataat gaactgaaag acttattcga caaagctgtc
     6901 acagaagacc tcaagaaagc tggaaactac tcaaatactg agaatttgaa atccaaagtc
     6961 ttcaaagctc ttcttaaaaa cacagacggt ttctttaatc cattgttcaa aaaagacatt
     7021 taaaccccaa ttattcaccc caaatctaaa aaccatccag aatccttgcc ttagcttaga
     7081 tcctggatgg tttctttttc acccaatggg tgttttttac tagacaaaaa agagtttccc
     7141 ctttatggta taagtgtaga aaaaaaacac aaaaagaaag gaaactcaca tgaacagttt
     7201 accaaatcat cacttccaaa acaagtcttt ttaccaacta tctttcgatg gaggtcattt
     7261 aacccagtat ggtggtctta tcttttttca ggaacttttt tcccagttga aactaaaaga
     7321 gcggatttct aagtatttag taacgaatga ccaacgccgc tactgtcgtt attcggattc
     7381 agatatcctt gtccagttcc tctttcgact gttaacaggc tatggaacgg actatgcttg
     7441 taaagaattg tcagctgatg cctactttcc aaaattgttg gaaggagggc agcttgcttc
     7501 acagccaacc ttatcccgtt ttctttccag aactgacgag gaaacagtcc atagtttgcg
     7561 atgcctcaac cttgaattgg tcgaattctt tttacagttt caccagctaa accaactcat
     7621 tgtagatatc gattctaccc atttcacaac ttatggcaag caagaaggtg ttgcttataa
     7681 cgcccactat cgtgctcatg gctatcatcc tctttacgct ttcgagggga agacaggtta
     7741 ttgtttcaat gcccagcttc gtcctggtaa tcgttattgt tctgaagagg cagacagctt
     7801 tatcacacct gttttagaac ggtttaatca acttctcttt cgaatggata gtggctttgc
     7861 gaccccaaaa ttatacgatt taattgaaaa aacagggcaa tactacctca taaaactcaa
     7921 gaaaaatact gttctgagct gtcttggaga cctttccctc ccttgcccac aggatgagga
     7981 cttaaccatc ttgccccact ccgcctactc agaaactctc tatcaagcag gatcttggtc
     8041 gcacaagcgt cgtgtctgcc agttctctga acgaaaagaa ggaaacttgt tctacgatgt
     8101 tatttctctc gttacaaata tgacgagtgg aacaagccaa gaccagtttc agctttatcg
     8161 tggacgtggt caagccgaga atttcatcaa ggagatgaag gagggatttt ttggcgataa
     8221 aatggatagt tcaaccttaa tcaaaaacga agttcgtatg atgatgagct gtatcgccta
     8281 caatctctat ctttttctca aacatctagc tggaggtgac ttccaaactt taacaatcaa
     8341 acgcttccgc catctttttc ttcacgtggt aggaaaatgt gttcgaacag gacgcaagca
     8401 gctcctcaaa ttgtctagtc tctatgccta ttccgaattg ttttcagcac tttattctag
     8461 gattagaaaa gtcaacctga atcttcctgt tccttatgaa ccacctagaa gaaaagcgtc
     8521 gttaatgatg cattaaagaa cagtcgagat gaaaaaatcg tgtgacgcac caagggagga
     8581 gtctgccctt ttgaggaaat ctagcgagga aaaacgatac tggaacagca gaaagtaaaa
     8641 ctgacctcat taggacatga ggtcaggggt tttgtaagtt acatctagtt gagagaggta
     8701 tgaatgattt gggttaaatg tttaaaacat aaagaggaca gttttttgct ccctctgaaa
     8761 agtcatcatt tgatggcttt tttctatata gggtaaaagg ctatcatcgg acaaaataaa
     8821 gaaggcatga tataatataa agtagatttt tatgtcataa aacaagaact gtttggacat
     8881 cattcatttt tagattgaga tctcttagtt ctagattaac agcttcagag ctacgaaagg
     8941 aaaaaactca atggctacta ttcaatggtt tcctggtcac atgtctaaag ctcgtcgaca
     9001 ggtgcaggag aatttaaaat ttgttgattt tgtgacgatt ttagtagatg cacgcttgcc
     9061 tctatctagt caaaatccta tgttgaccaa gattgttggt gataaaccaa aactcttgat
     9121 tttaaacaag gccgacttgg ctgatccagc aatgaccaag gaatggcgtc agtattttga
     9181 atcacaagga atccagacgc tagctatcaa ctccaaagag caagtgactg taaaagttgt
     9241 aacagatgcg gccaagaagc tcatggctga taagattgct cgccagaaag aacgtgggat
     9301 tcagattgaa accttgcgta ccatgattat cgggattcca aacgctggta aatcaactct
     9361 gatgaaccgt ttggctggta aaaagattgc tgttgttgga aacaagccag gggtcacaaa
     9421 aggtcaacaa tggcttaaaa ccaataaaga cctggaaatc ttggatacac cggggattct
     9481 ctggcctaag tttgaggatg aaactgttgc acttaagttg gcattgactg gagctatcaa
     9541 agaccagttg cttcctatgg atgaggttac catttttggt atcaattatt tcaaagaaca
     9601 ttatccagaa aagctggctg aacgcttcaa acaaatgaaa attgaagaag aaccgtctgt
     9661 gattattatg gatatgaccc gcgccctcgg tttccgtgat gactatgacc gtttttacag
     9721 tctcttcgtg aaggaagtcc gtgatggcaa actcggtaac tataccttag atacattgga
     9781 agacctcgat ggcaacgatt aaagaaatca aagaactcct tgtgacagtc aaggagttag
     9841 aaagccctat ttttttagag cttgaaaagg ataatcgctc aggagttcaa aaggaaatca
     9901 gcaagcgtaa aagagccatc caagctgaat tagatgaaaa tttgcgcttg gaatccatgc
     9961 tttcttatga aaaagaactt tataagcaag gattgacctt aattgcaggt attgatgagg
    10021 ttggtcgtgg tcctcttgct ggtcctgtag tcgctgcggc cgttatttta cctaaaaatt
    10081 gtaagattaa aggtctcaac gacagcaaga aaattcctaa aaagaaacat ctggagattt
    10141 tccaagccgt tcaagaccaa gccttgtcga ttggaattgg tatcatagat aatcaggtca
    10201 tcgaccaagt caacatctat gaagcaacca aactagccat gcaagaagca atctcccagc
    10261 tcagccctca accagagcac cttttgattg atgccatgaa actggacttg cccatttcac
    10321 aaacctccat tatcaaagga gatgccaact ccctctctat cgcagcagca tctatagtag
    10381 ccaaggtaac acgtgatgaa ttgatgaaag aatacgatca gcagttccct ggctatgatt
    10441 tcgctactaa tgcaggatat ggcacagcta aacatctgga aggcctcaca aaactaggag
    10501 ttaccccaat tcaccgaacc agctttgaac ccgttaaatc actggtttta ggtaaaaaag
    10561 aaagttaatt gaaaggaaat accatggagg aacagtcgga aatagtccgt tctaagaaag
    10621 aattcgcctt tgcatccagc actatactat cccaagttgg tcgaggaatc attgtcggcc
    10681 tcatcgttgg aattatcgtc ggatcctttc gtttcttaat tgaaaagggc ttccacatga
    10741 tacaaggagt ttatcaagat caagggtact tagtgcgcaa tctttttgta ctggttttgt
    10801 tttatatact catctgttgg ctcagtgcca aactaacacg gtcagaaaaa gatattaaag
    10861 gctcaggaat tcctcaagtc gaagccgaac tgaaaggcct catgtccctc aactggtggg
    10921 gcattctttg gaaaaaatat gtgctaggta ttctcgctat tgccagtgga ctcatgctgg
    10981 gtcgagaggg acccagtatt caacttggag cagttggtgg taaaggaatt gccaagtggc
    11041 tcaaatccag tccagtagag gaacgttcct tgattgccag tggagctgca gcaggtttag
    11101 ccgcagcctt taatgctcct attgcagcac ttctctttgt tgtagaagaa gtctatcacc
    11161 atttttcgcg ctttttctgg gtctcaactc tagcagccag catcgtagca aactttgtgt
    11221 ctctactcat gttcggtttg acaccagtat tggatatgcc agataacatt cctcccatga
    11281 ccctagatca gtattggata tatctcgtca tgggaatttt ccttggattt tcaggctttc
    11341 tctatgagaa agctgtatta aacgttggaa gagtttatga cttgattggt caaaaaatcc
    11401 atttggatag agcttattat cccatcttgg cttttatcct tatcatacca gtcggaatct
    11461 tcttacctca aatcattggt ggcggaaatc agcttgtcct ttctttaact gaacaaaatt
    11521 ttagtttcca agttttatta gcttactttt tgatccgctt tatttggagt atgattagct
    11581 atggaagtgg actgccagga ggaattttcc tccccatttt agctcttggt tctttgcttg
    11641 gtgccttaat tggtgttatc tgtgtcaatc ttagacttgt cagtcaagag caattcccta
    11701 tatttgtcat tctaggaatg agtggctatt ttggagccat atcaaaagct cccttaaccg
    11761 ctatgatcct cgtaactgag atggtaggag atattcgcaa cctcatgcca cttggtcttg
    11821 tcactcttgt ttcttatatt atcatggatt tgctcaaagg tacgccagtc tatgaagcca
    11881 tgctggaaaa aatgcttcca gaagaagtat ctagcgaagg agaagttaca cttatcgaaa
    11941 taccagtttc tgataaaatt gctgggaaac aagttcatga actcaactta ccacacaacg
    12001 tcctcatcac aactcaagtc cataatggca agagccaaac agttaacggc tcaaccagaa
    12061 tgtatctggg tgatatgatt cacctggtta ttccaaaaag tgaaattgga aaagtcaaag
    12121 atttgttgtt gtagtacaat catttacata atttatatta tgtaaatgat tggcttgatt
    12181 tatttagaaa a
//
LOCUS       DBERCA010000050        12156 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.050, whole genome shotgun sequence.
ACCESSION   DBERCA010000050 DBERCA010000000
VERSION     DBERCA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.050"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..224
                     /locus_tag="ACL719_001586"
     CDS             <1..224
                     /locus_tag="ACL719_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699523.1"
                     /translation="KRAILHQENALYFRFFSNFDDFLEKNYLKTIWQVSKETPFSEMD
                     FNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            360..1013
                     /locus_tag="ACL719_001587"
     CDS             360..1013
                     /locus_tag="ACL719_001587"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGS0699524.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            1023..1487
                     /locus_tag="ACL719_001588"
     CDS             1023..1487
                     /locus_tag="ACL719_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGS0699525.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPELDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1500..2768
                     /locus_tag="ACL719_001589"
     CDS             1500..2768
                     /locus_tag="ACL719_001589"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGS0699526.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2782..3042
                     /locus_tag="ACL719_001590"
     CDS             2782..3042
                     /locus_tag="ACL719_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699527.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            3053..4414
                     /gene="pdrM"
                     /locus_tag="ACL719_001591"
     CDS             3053..4414
                     /gene="pdrM"
                     /locus_tag="ACL719_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGS0699528.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4568..5536
                     /locus_tag="ACL719_001592"
     CDS             4568..5536
                     /locus_tag="ACL719_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGS0699529.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5552..6544
                     /locus_tag="ACL719_001593"
     CDS             5552..6544
                     /locus_tag="ACL719_001593"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGS0699530.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6762..7805
                     /locus_tag="ACL719_001594"
     CDS             6762..7805
                     /locus_tag="ACL719_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGS0699531.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGIQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7851..9554
                     /gene="lpdA"
                     /locus_tag="ACL719_001595"
     CDS             7851..9554
                     /gene="lpdA"
                     /locus_tag="ACL719_001595"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGS0699532.1"
                     /translation="MALEIIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            9618..10607
                     /locus_tag="ACL719_001596"
     CDS             9618..10607
                     /locus_tag="ACL719_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGS0699533.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10674..11744)
                     /gene="xerS"
                     /locus_tag="ACL719_001597"
     CDS             complement(10674..11744)
                     /gene="xerS"
                     /locus_tag="ACL719_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGS0699534.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3636 a         2253 c         2691 g         3576 t
ORIGIN      
        1 taaaaagagc gattttacac caagagaatg cattgtattt tcgatttttt tctaattttg
       61 acgacttttt agaaaaaaat tatttaaaga ctatatggca agtttctaaa gaaactcctt
      121 tttctgaaat ggattttaat atgtttaaaa atatttcaga aaagataatc ttcgaaagag
      181 gtagtaaaat gcttaatgat ttaaaaagta attataaaaa atagccagtc tttataaact
      241 gtattcacgt attcaaatcg tatatagtcg atttattaaa tctttagata aactgtaaaa
      301 agtggtttcc atagccactt tttgctataa tagaggcagt aaaaatgaag gagtcggcta
      361 tggaacactc gtcttggcat gctttgatta aggcgcaatt acctgagggt tatttcggga
      421 aaatcaatca gtttatggag caggtctatt ctcaggggat tatttatcca cccaaggaaa
      481 aggtttttca ggctctcttg acaacactgc ttgaagaagt taaggtggta attctagggc
      541 aagaccccta tcacggacca ggtcaagcgc agggcttgag tttttccgta cctgactcta
      601 tcccagctcc accatccttg caaaatatct tgaaagaatt gtcagatgat atcggagtta
      661 agaaatctca tgatttgaca gcttgggctg agcaaggagt cttgcttctt aatgcttgtt
      721 tgactgttcc tgctggacag gccaatggtc atgctggtca aatctgggag ccttttactg
      781 atgctgtgat tcaggtggtc aatcatctag atagaccagt cgtttttgta ctctggggag
      841 cttatgcacg taagaagaag gccttagtta ccaatcctca tcacttgatt attgaatcag
      901 cccatccaag tcctttgtcg gtttatagag gattttgggg ttccaagcct ttttccaagg
      961 ccaatacatt cttaaaagag acaggacaag agccaatcga ttggcttaga taaggagaaa
     1021 atatgcctca gttagcgacg atttgttaca ttgataatgg gaaagaactg ctcatgctcc
     1081 atcgtaataa gaaacccaat gatgtccatg aagggaaatg gattggtgtg ggtggtaagc
     1141 tagagagagg agagacgccc caggaatgcg cggtacgtga aatccttgaa gaaacagggc
     1201 tcaaagccaa gccagttcta aaaggtgtca tcacttttcc tgaatttaca ccagaattag
     1261 actggtacac ctatgttttt aaggtgacgg agtttgaggg cgacttgatt gactgcaatg
     1321 aggggatgct agaatgggtt ccctatgatg aggttttgag caagccgact tgggaaggtg
     1381 accacacctt tgttgagtgg cttttagagg ataaaccctt cttttcagcc aagtttgttt
     1441 atgatgggga taaattgttg gatacccaag ttgatttcta tgaataaagg agaaagcaga
     1501 tgctactaat caaaaatggt cgtgtaatgg atcccaagtc tggtttggat caagtttgtg
     1561 atgtcttagt tcaagatggg aaaattatca aaattgcgcc tgagatcacg gaagaaggag
     1621 cagaaacgat tgatgctact ggtcatgtgg ttgctcctgg tttggtcgat attcatgtgc
     1681 atttccgtga acctggtcaa acacataaag aagacattca tactggtgcc ctagcagccg
     1741 ctgcaggtgg ttttactact gtcgtcatga tggctaatac cagtccaacc atttcagacg
     1801 tggagacttt gcaagcagtt ctccagtcag ctgccaaaga gaagattaat gtcaagacag
     1861 ttgcgaccat tactaaaaac tttaatggta aaaacttgac tgactttaag gcacttttag
     1921 aagctggtgc ggttggtttc tctgatgacg gtattccgct tgagagcagt aagattgtca
     1981 aggaagccat ggaggaagcc aaaaaactca atacctttat cagccttcat gaggaagatc
     2041 caggtttgaa cggtgttctt ggctttaatg aaaatattgc tagagaacat ttccatatct
     2101 gtggtgctac tggggtggct gagtacgcta tgatggcgcg tgatgtcatg attgcctatg
     2161 caactaaagc ccatgttcac atccagcatt tgtctaagga agaaagtgtt aaagtagtgg
     2221 agtttgctca ggggttaggt gcagaagtca cagcagaagt agcaccacag catttctcta
     2281 agacagaagc acttctttta acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt
     2341 tggaatcaga ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc acagttattg
     2401 cgactgacca cgcgcctcat catgtagatg aaaaaaatgt tgaagatatt accaaagcgc
     2461 catctggtat gactggctta gaaacatccc tgtctctcgg cttgacctat ttggtagaag
     2521 ctggtgagtt gagcttgatg gaattacttg aaaaaatgac atacaaccca gccaagcttt
     2581 acaactttaa agcaggttac ttggctgaga atggtccagc agatatcact atttttgatg
     2641 ccaaggctga ccgccttgtg gactcccatt ttgcttccaa agcagctaat tcaccattca
     2701 tcggtgaaac cttaaaaggg caggttaaat ataccatctg taagggacaa atcgtctatc
     2761 aagcttgata ggagttagaa tatgaattat tttcgaaaac gtagggagag acaagcgaaa
     2821 tctaatagtg gaatttactg tcccgcagct aatcgttcca aaattcaata tgaaactaaa
     2881 gaaaaagctg accatgcggt tcaatatgat aacggaggtt tagttagaag ctattattgt
     2941 cgtacatgtg cgtgttggca tactacttca aaatctaata aacctccgct aagtttgaaa
     3001 aactatggtc tgaaactctt tggtttagat aaatcagaat agaaagagaa gtatgtataa
     3061 gacaaagtgt ttacgagaga agttagtatt atttttaaaa attttcttcc caatcctgat
     3121 ctaccaattt gccaattatt ctgcctcttt tgttgatact gcaatgacag gtcaatacaa
     3181 cactatggac ttggctggtg tatctatggc aaccagtatc tggaatcctt tctttacatt
     3241 tctaacaggg attgtgtcag ccttggtgcc tatcattggt caccatcttg gtcgaggcaa
     3301 aaaggaagaa gttgcgtctg atttttacca atttatttat ttggccttgg gcctatctgt
     3361 ggtcttgctg gggatggtac ttttcttggc accaacaatc ttgaatcata ttgggttaga
     3421 agcagcagta gcggcagtag cggttcgcta tctttggttt ttatctatcg ggattatccc
     3481 cttgttgctc tttagcgtca ttcgttcctt gctggattcg ctgggcttga ccaaactgtc
     3541 catgtacctc atgcttttgt tactccctct caatagcgga tttaactatc tcttgattta
     3601 cggtgccttt ggtgttccag aactgggagg ggctggtgct ggtttaggaa catccttggc
     3661 ctactgggtc ttgcttggga tttctgttct ggttttattt aaacaggaga agctcaaagc
     3721 cttacacctt gagaaacgaa ttccacttaa tatggataac attaaggaag gagttcgttt
     3781 aggtctgcct attgggggaa ctgtcttcgc ggaagtggct atcttttcag tggttggctt
     3841 gattatggct aagttttcgt ccttgattat agctagtcac cagtcagcta tgaacttttc
     3901 aagtcttatg tacgcctttc ctatgagtat ctcatcggct atggctattg tcgtttccta
     3961 tgaagtggga gccaagcgat ttgatgatgc gaaaacctat attggtctag gaagatggac
     4021 tgctctcatt tttgcggcct tcaccctaac cttcctttac atttttaggg gaaatgtggc
     4081 cagtctttat ggtaacgacc caaaatttat cgatttgaca gcgcgttttt taacttatag
     4141 tcttttcttc cagttagcag atacctttgc ggcaccgctt cagggaattt tgcgggggta
     4201 taaggataca gttattcctt tttaccttgg tttgcttggt tattggggcg tagcaatccc
     4261 tgtggctacg ctatttgatt ccctaacaga ttttggagct tattcttact ggatcggctt
     4321 gattattagt ttgattgtga gtggggcgct ctaccgttgg cgtttaactg tgattatgaa
     4381 gagatttgaa tctttagcaa aatccaaacg ctaaaaagtt tgtgaaaaaa tatccttgac
     4441 aagaaaatcc cttgttttca cggggttttc ttttttattt tgtgaaattt cttttagaaa
     4501 aactttgaca gtatttgcca aaaacggtaa aatagtaagg taagaagaaa gttagaaagg
     4561 caagaagatg tcaactttag ataaaaatct tttgctagaa atgttccgta agatggaaga
     4621 aatccgtcgc atggacttaa aaattgcaca attagtaaag aaagggaaag tgccaggaat
     4681 gacgcacttt tctgttggtg aagaggcagc taacgtgggg gctatgttag ctctcaatcc
     4741 agatgatctg attacctcaa accaccgtgg acacgggcaa gctattgcta aagggattga
     4801 cctcaacgga atgatggctg aaatccttgg taaatacact ggaacctgta aagggaaagg
     4861 tggatctatg catatcgctg accttgatgc tgggaacctt ggtgccaatg gtatcgtagg
     4921 tggtggtatg gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa
     4981 aatcgttgtt tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt
     5041 gaacatggct tctatctgga acctgccagt cattttctat tgcattaaca acggttacgg
     5101 tatctctgcg gatatcaaga aaatgaccaa tatagaacat atccatcaac gtagcgccgc
     5161 ttatggaatt cctggaatgt tcatcgaaga cggtaacaat gtcatcgatg tctatgaagg
     5221 atttcagaaa gctgtagacc atgttcgcag tggcaatggt ccagtcttga ttgaaagtgt
     5281 aacttatcgc tggctcggtc actcatcatc tgaccctggt aaatatcgta cgcgtgaaga
     5341 agtggaattg tggaaacaaa aagatccaat cgaaaacctc cgcaattacc ttattgaaaa
     5401 taacattgca agtgccgaag aattggaaga aatccaagcg caagtaaagg aagcagtaga
     5461 agcttctgtt aaatttgcag aggaaagccc attcccaccg cttgaatcag cctttgaaga
     5521 tatttacgca gactaaggag aaagagataa aatggaaaca aaaacaatgt ccttccgtga
     5581 caccattatc cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg
     5641 agaagacgtc ggtgtcttcg gaggagactt cggtacttct gttggaatgc ttgaagaatt
     5701 tggtccagaa cgcgtccgtg actgtccgat ttctgaagct gccatctctg gagcagcagc
     5761 aggagcagcc atgacaggac ttcgtccaat cgtcgatatg accttcatgg acttctctgt
     5821 tattgccatg gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa
     5881 aggtcaggtt ccaatgactg ttcgttgtgc agctggtaac ggagttggct ctgccgccca
     5941 gcactcacaa tctctagagt cttggtttac tcacattcca ggacttaagg ttgtggcacc
     6001 tggaacacct gcggacatga aaggcctgct taagtcttct atccgtgata aaaacccagt
     6061 tattatactt gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga
     6121 ctacacaatt ccacttgggg ttggtgaaat caaacgccaa ggaacggatg taacagttgt
     6181 tacttatgga aaaatgcttc gccgtgtggt tcaagctgct gaagaattag cagaagaagg
     6241 aatttcagtt gaaattgttg acccacgtac ccttgttccg cttgataagg atatcatcat
     6301 aaactcagtg aagaagactg gtaaggttgt tctggtcaac gatgcccaca aaacaagtgg
     6361 ctatatcggt gaaatttcag ctattatttc agaatcagaa gcatttgact atctagatgc
     6421 accaatccgc cgttgtgcag gagaagatgt gccgatgcct tatgcacaaa acctagaaaa
     6481 tgcaatgatt ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga
     6541 atagagttac ataagataag ttatgtaaat aaaataaagc tgacgagaaa ctttgtatct
     6601 tttaggtgca gagctcttcg tcattaatat cttagattag agcacgggct aaaagctcgg
     6661 aaaaaagata aatctccttg gcttcatcga agccatcgtc gatttcctat ttttcagtcg
     6721 cttttgacgc cctttgtatc ataaatagaa ttggagaggt catggctgat gacaagctaa
     6781 gagcgactcc tgcagctaga aagttagcgg atgatttagg gataaacctc tacgacgttt
     6841 ctggctcagg tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat aaagacacaa
     6901 acgtggttcg catttcgcca cttgcaaaac gaattgccct cgaacataac attgcttggc
     6961 aggaaatcca aggaaccggt catcgtggta aaatcatgaa gaaggatgtt ttggccctgc
     7021 ttcctgaaaa tatcgaaaac gatagcatca aatctcctgc tcagattgaa aaagtggaag
     7081 aagttcctga taacgtaaca ccatatggtg aaatcgagcg tattccaatg acaccaatgc
     7141 gtaaggttat tgcccaacgc atggttgaat cttacttgac tgcgccaacc ttcaccctca
     7201 actatgaagt tgatatgact gaaatgttgg ctcttcgtaa gagggttctt gagccaatca
     7261 tggaagcaac tgggaagaag actactgtaa cagaccttct ttcacttgca gttgttaaga
     7321 ctcttatgaa acatccatac atcaacgctt cattgacaga agatggcaag actattatca
     7381 ctcacaacta tgtcaatctt gctatggcag ttgggatgga taatggattg atgacacctg
     7441 ttgtttacaa tgctgagaag atgagtcttt cagaactggt tgtagccttt aaggatgtta
     7501 ttggccgtac cttggatggt aaattggctc caagtgagtt acaaaattca acattcacaa
     7561 tcagtaattt gggaatgttt ggtattcagt cctttggtcc gattattaac caacccaact
     7621 cagctatcct tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg
     7681 tgattcgccc aatcatgagt ttaggattaa ccattgacca ccgtgtcgta gatggtatgg
     7741 ctggtgctaa gtttatgaag gacttgaaag agttgattga aaccccaatc tcaatgttga
     7801 tttaagagca agagtattca tttttaagat atagataaag gaaggaagac atggccttag
     7861 aaataattat gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc caatggaata
     7921 aaaaagtcgg agaatttgta aaagaaggag aaatcctttt ggaaatcatg actgataaag
     7981 tcagcatgga attggaagcc gaagaagatg ggtacttgat tgccattctc aaaggagatg
     8041 gtgaaactgt ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc
     8101 caacagctgg agcagcatcg ccagaagcta gccctgtacc tgtagcaagt acctcaaacg
     8161 atgatggtaa gagcgatgat gcttttgata tcgttgtgat tggtggaggt cctgctggtt
     8221 atgttgcagc cattaaagct gcccaactcg gcggtaaggt tgcccttgtt gagaaatctg
     8281 aacttggtgg aacctgctta aaccgtggat gtattccaac caagacctac cttcataacg
     8341 ctgaaattat tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta
     8401 acttcactgt tgatatggaa aaacttttag aaactaaatc taaagttgtt aatactcttg
     8461 ttggtggtgt tgcaggactt cttcgtagct acggagttac tgttcataaa ggaattggta
     8521 caatcactaa agacaagaac gtcttggtaa atggctctga attgcttgaa accaagaaaa
     8581 tcattcttgc tggtggttca aaagtcagca agatcaatgt ccctggtatg gaatctccac
     8641 ttgtcatgac tagtgatgac attcttgaaa tgaacgaagt gccagaaagc cttgttatca
     8701 tcggtggtgg agttgtcggt atcgaactcg gtcaggcctt catgacattt ggttcaaaag
     8761 tgactgttat cgaaatgatg gaccgtatag ttccagctat ggatgcggaa gtttctaaga
     8821 accttcgctt gatccttgag cgtaaaggaa tgaccatctt gactggtact aaactgcaag
     8881 aaatcattga ggaaaatggt caacttcgta tcaaggttga aggaaaagac gatatcatcg
     8941 caagcaaagc tcttctttca attggtcgta tgccagacct tgaaggtatt ggagaggttg
     9001 agtttgaatt ggatcgtggt tgtatcaagg tcaatgaata catggaaact tcagttccag
     9061 gcatttatgc accaggtgac atcaacggta ctaagatgtt ggctcacgca gctttccgca
     9121 tgggtgaagt ttccgctgaa aatgccctta aaggaaatca tgcagttgcc aaattgaatt
     9181 tgactcctgc agccatctac actctccctg aagtagcagc agtaggtttg acagaagaac
     9241 aagcccgtga gaaatacgat gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg
     9301 ctattgcatc tgacgcagct caaggtttcg taaaagttat cgctgataag aaatacggag
     9361 aaatccttgg tgtacacatt attggtcctg cagccgcaga attaataaac gaagcatcaa
     9421 gcatcatcga aatggaaatc actgttgagg aaatgctgaa gaccatccac gggcacccaa
     9481 cttactctga agtgatgtac gaagcgtttg cagatgttct aggaatggcc atccattcac
     9541 ctaagaaaaa ataaaacgaa gatagattag actggaaggt tatttccagt ctctatcata
     9601 taaagtaaag ggatatcatg aaatacatta tcaatcattc aaatgacact gcttttaata
     9661 ttgccttgga agaatacgcc tttaaacacc ttttggatga ggatcaaatc ttcctacttt
     9721 ggattaacaa gccatctatc attgttggtc gtcaccagaa cactatcgaa gaaatcaacc
     9781 gtgattatgt tcgcgaaaat ggtattgagg tagttcgccg tattagtggt ggtggagctg
     9841 tttaccacga tttaaataac ctcaactaca cgatcatctc caaagaagat gaaaataagg
     9901 catttgactt caagagcttc tcaactccgg ttatcaatac cctggctcaa cttggggtta
     9961 aagctgagtt cacaggtcgt aatgaccttg agattgatgg taagaagttc tgtggcaatg
    10021 cccaagccta tatcaacggc cgtatcatgc accacggttg cttgctcttt gacgttgatt
    10081 tgtcagtcct cgcaaatgcc cttaaggttt caaaagataa atttgaatca aaaggtgtga
    10141 aatccgtccg tgcccgtgta accaatatta tcaatgaatt accaaaaaaa atcacagtcg
    10201 aaaaattccg tgatttactc ctggaataca tgaaaaaaga gtacccagag atgactgaat
    10261 acgtcttttc agaagaagaa ttggccgaaa tcaatcacat caaggatact aagtttggaa
    10321 cttgggactg gaactatggt aaatcacctg aatttaacgt ccgtcgtgga accaaattca
    10381 ctagtggtaa ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa gacatcaaga
    10441 tttatggtga cttctttggt attgaagacg ttgctgcagt agaagatgtc cttcgtgggg
    10501 tgaaatacga acgcgaagat gttcttaagg cgctaaaaac cattgatatc acacgctact
    10561 tcgctggtat tagccgtgaa gaaatcgctg aagcggtagt tggataaatc aaaaaaagaa
    10621 gttggccaac ttcttttttt gatatttatt tacataattt aaaatacgta aaatcataaa
    10681 ctatctagag cattcttttg ttcatcatta acaatatggg tatagaggtc agtgacttgt
    10741 gtgctggcat gtcctagttg gtgactgact aaaacttgtg atttagtcgc atcatagagc
    10801 ctagttgcta gcgtatggcg cagtttatgg ggtgttacac gcactttaaa atcctctgag
    10861 tatttagcaa ccattttctc aacgctagaa gcatcgatac gattaggaac acctctgtag
    10921 agagttaaaa aaagggctgt atctgttttt tccgttttat agcgttgatt ccgaatggcc
    10981 agataattct ctaaataagg tttagcaaaa gcagcgacat tgactgagtc acgtttgcca
    11041 ccttttcgag taacatcaat aaccatcatt tttagattga gatctcttag atctagatta
    11101 acagcttcag ataagcgaac accagatgcc aagagaaggg caataatggc taaatctcgt
    11161 tctttatttt tgttgaatga tgagagagct cgatttgaaa gctgttgtgg gtgctcttga
    11221 tcgatataag ttagaaaacc ttctgtttca tcacctagaa agagtttttg cttgatattt
    11281 tcagctctgg cagcaagggt ttctttcttt ttcttggttg aaactttttt cattacatta
    11341 cgatagaaat aaggttcccc ctgatcgttt tcaacctcct cggttagata cttgtaaaga
    11401 ctagaaagtg ctgataaggt tcgattgata gttgtctgtg aaacaccttg ttttgttgta
    11461 ttagcattca gcaagggacg ttcacgtaga taaaggataa aggattccat gtctttctta
    11521 gacatatttt ccaaaactga taaaggaata tcggatattt tatcagcgtt tgaaatacca
    11581 gactccaaaa cccagctgaa aaatcggtca tattccttaa ggtattcgta cagggttgta
    11641 aaactgtagg gcacagccag cttagattgg tagtattcca gaacatacca gggcatgagt
    11701 tgttttagtt tgtcgattcg ttccagtaaa atctcacgtt tcatgtattt tctccataag
    11761 taagtatact agtagtatag catagacttg tatatttttc aagaaaatta tagtttttct
    11821 gaaagatgtt ataggagttt ttcaaagtga taaaaagact tagacctaca aacaaaacag
    11881 aataaggaaa ataataaaaa tctcacccac taaacttctg ttaatactac ataactgaag
    11941 agctatataa ggagctatga gatacaaaac acattgtata atagattgaa actagaatag
    12001 tacatctgaa cttctaaaac attgctagaa attgatttga ttgtcctgat tgttttatcc
    12061 tatttctatt tcatttcact atataattgc tattgttttc tttttcatga ttttaatctt
    12121 actgtacaga tttgatacag taaactcctt tctagc
//
LOCUS       DBERCA010000051        12133 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.051, whole genome shotgun sequence.
ACCESSION   DBERCA010000051 DBERCA010000000
VERSION     DBERCA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.051"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(105..2363)
                     /locus_tag="ACL719_001598"
     CDS             complement(105..2363)
                     /locus_tag="ACL719_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0699535.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2592..2822)
                     /locus_tag="ACL719_001599"
     CDS             complement(2592..2822)
                     /locus_tag="ACL719_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGS0699536.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3012..3695
                     /locus_tag="ACL719_001600"
     CDS             3012..3695
                     /locus_tag="ACL719_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0699537.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3695..4429
                     /locus_tag="ACL719_001601"
     CDS             3695..4429
                     /locus_tag="ACL719_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0699538.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQILSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4583..5440
                     /locus_tag="ACL719_001602"
     CDS             4583..5440
                     /locus_tag="ACL719_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGS0699539.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5582..6454
                     /locus_tag="ACL719_001603"
     CDS             5582..6454
                     /locus_tag="ACL719_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGS0699540.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6736..7419
                     /gene="rpiA"
                     /locus_tag="ACL719_001604"
     CDS             6736..7419
                     /gene="rpiA"
                     /locus_tag="ACL719_001604"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGS0699541.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7433..8644
                     /locus_tag="ACL719_001605"
     CDS             7433..8644
                     /locus_tag="ACL719_001605"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGS0699542.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8646..9188
                     /locus_tag="ACL719_001606"
     CDS             8646..9188
                     /locus_tag="ACL719_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGS0699543.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9204..10013
                     /locus_tag="ACL719_001607"
     CDS             9204..10013
                     /locus_tag="ACL719_001607"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS0699544.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10489..10884
                     /locus_tag="ACL719_001608"
     CDS             10489..10884
                     /locus_tag="ACL719_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGS0699545.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10894..11469
                     /locus_tag="ACL719_001609"
     CDS             10894..11469
                     /locus_tag="ACL719_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGS0699546.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDGSGKEIE"
     gene            11494..12006
                     /locus_tag="ACL719_001610"
     CDS             11494..12006
                     /locus_tag="ACL719_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGS0699547.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3590 a         2220 c         2751 g         3572 t
ORIGIN      
        1 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
       61 aaagaagcag aaaaatttca ttttctactc cttttctata ggatttattt ttctttttca
      121 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      181 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      241 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      301 tcaaccaact tttccttgac cttatcagtt acatccaaac gaatgttgtt gctagagagg
      361 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      421 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      481 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      541 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      601 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      661 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      721 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg ggcgccgacc
      781 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      841 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
      901 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
      961 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1021 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1081 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1141 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1201 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1261 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1321 gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1381 gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg
     1441 tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact
     1501 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1561 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1621 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1681 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1741 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1801 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1861 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     1921 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     1981 tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct
     2041 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2101 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2161 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2221 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2281 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2341 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2401 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2461 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2521 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2581 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2641 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2701 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2761 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2821 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     2881 tagaaaaaac taagatttct cgctaattct gctagtttaa agatttttaa ataaattgga
     2941 taaaaaattt aaattttaat tcaaaacatg ttataatcat acagtattct attttagaaa
     3001 gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt aactatgggg
     3061 ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt ttgggtgttg
     3121 tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc aacttgtacg
     3181 tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt gctcttatgc
     3241 atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg cgtctgattc
     3301 cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagact gttcgtgccg
     3361 gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg attcgtccta
     3421 aaaatgcgat gcgttatgtg attttgccac aagcagtcaa aaatatcttg ccagcattgg
     3481 ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt ggggtcatgg
     3541 agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta acaccacttt
     3601 tatttgcagc attttactac ttgattatga cctctattct gacagtagcc ttgaaagctt
     3661 ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga taaaaattga
     3721 aaatttacat aaatcttttg gaaagaatga agtattgaag ggcatcaacc tcgagattaa
     3781 aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta ccttgcttcg
     3841 ctctatgaat ttgttggagg aagcaaccaa ggggaagctt atctttgagg gagtcgatat
     3901 tacggacaag aagaatgacc tgtttgccat gcgtgagaag atgggcatgg ttttccaaca
     3961 atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac ctatcaagac
     4021 taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg aaaaagttgg
     4081 tttgccagat aaggcagacg cttatccaca gattctgtcg ggtgggcaac aacaacggat
     4141 tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg aaccaacttc
     4201 agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc ttgccaagtc
     4261 agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg tggcagatcg
     4321 tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc agatttttga
     4381 acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat ctattttaaa
     4441 atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt ttgaaactat
     4501 aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata atggaagata
     4561 tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt tagcggccaa
     4621 attgcagggg cagttggctg aaaagactgc aaaattaaag gaggaaacag ctctagtgcc
     4681 tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg ttcgcaacaa
     4741 ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc cagagactat
     4801 tactcaagag gaattgttag acctgattgc taaatataat caggatccag cttggcatgg
     4861 gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt tattagccat
     4921 tgacccagaa aaggatgtgg atggtttcca tcccctaaac atgggacgtc tttggtctgg
     4981 acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc atgaatatgg
     5041 gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt tcaaatatcg ttggaaaacc
     5101 tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttggcccact cacgaactca
     5161 taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg gccgcgccaa
     5221 gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg ggatgaatcg
     5281 agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac cacttgctag
     5341 ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc tgatggagca
     5401 aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaaacatt cgattaatag
     5461 agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt ttagatataa
     5521 gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag aggaaaaaag
     5581 gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt ctcgttcaag
     5641 tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt atggaggtgc
     5701 catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattggtga ccgttggaac
     5761 ggacagggaa aatatcccgg ctctgcacag ccatttacct gagtctatgg ccttttctct
     5821 tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttgtct tgctggggcc
     5881 tggtttacga gacgatgctt ctggagaaaa tctagtaaaa caggtctttg ttaatttaag
     5941 ccaaaatcag attttgattg tagacggagg tgccttgacc attcttgcta ggacaagttt
     6001 gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg aaaaattatc
     6061 tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga cttccttccc
     6121 tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag ttggccaatc
     6181 tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa tgggagatac
     6241 tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc tctacgagcg
     6301 tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc aagaaaatta
     6361 tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa ttatttgtca
     6421 acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc tacttccttc
     6481 tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg tatcaaagtt
     6541 aagagtttgt ttttgtaact ggaaagagat gaacaaatca aatacataaa agataaactc
     6601 atttttattc gtttggtaaa aataaaatgc atatttttaa agaaagattg atgaaaaatg
     6661 tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag aattgagaat
     6721 gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc tgctgagttc
     6781 gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta ttttgtcgaa
     6841 gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac aacttctagt
     6901 gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga ccaagtagac
     6961 tttgtcgatg tgacggttga tggcgcagat gaagtggata gtcagtttaa tggaatcaaa
     7021 ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa agaatacatt
     7081 tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt gccagtagaa
     7141 gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg ctacaaacca
     7201 agtttccgtg aaaaagacgg ccaacgtttt gtgacggata tgcagaattt tatcattgac
     7261 ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga ccatgtcgtt
     7321 ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt tgctggacga
     7381 gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata agatgtctaa
     7441 atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag caccagatgc
     7501 taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg gacacatttc
     7561 aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa atattcctcg
     7621 tgaaactcct cttaagactg tagcagctga aatcaatcca actggatatg caacaaaatt
     7681 agaggaagta tctcttggta aggatactat gactggacac tgggaaatca tgggactcaa
     7741 cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc tgacaaaaat
     7801 tgaagaattc tcaggacgca aggttattcg tgaagccaac aaaccttatt caggaacggc
     7861 tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta tctatacttc
     7921 agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg atgaattgta
     7981 ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc ttggtcgcat
     8041 cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa accgtcgtga
     8101 cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg ctgatatcga
     8161 tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca accatgacat
     8221 gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta tgggacttgc
     8281 tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc tttacggcca
     8341 tcgtcgtaat gctcacggtt accgtgattg cttgcatgag tttgatgaac gcttacctga
     8401 aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc atggaaatga
     8461 cccaacgtat gcaggaacgg atcacactcg ggaatatatt ccattgttgg cctatagccc
     8521 tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt cagcgactgt
     8581 tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag ataaattggt
     8641 ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtagt aagaataagg
     8701 tcgtcatggc ggagcttcat caagaattga caaacttggg actggaaaag gttgagagct
     8761 acatcaacag tggcaatatt ttctttgctt cgatagattc caaagcccaa ttggttgaaa
     8821 agctagagac tttctttgca gtccattatc catttattca gagcttttct ttactgagtc
     8881 tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga gacttggcac
     8941 gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc gcgacagttg
     9001 aaagtttaga gctgaaagat gaagtgcttt attttggaaa acttgggatt ttctggggga
     9061 aattttctga agaatcctat tctaagactg cctatcagaa gtatttgctg aaggtgcctt
     9121 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     9181 aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt
     9241 tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttggg tcaggacttg
     9301 gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact
     9361 ggggccgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc
     9421 gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg
     9481 tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg
     9541 cagctggcgg tatcagattt ggtcctggta ccttgatggc tatctcagac catatcaaca
     9601 tgacggggca aaatccattg atgggggaaa acttggatga ctttggccca cgtttcccag
     9661 atatgtctag ggcctacaca ccagaatacc gtgccactgc ccatgaagtg gctaaaaaac
     9721 ttaatatcaa gattgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac
     9781 cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc
     9841 ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta
     9901 actttgcggc tggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac
     9961 gtgttaaagg tgatttcaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa
    10021 gatttaaaag ggggagtgcc tctgtctttt caggattgac tgcctatccg tattaaagaa
    10081 gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata
    10141 ggtgtgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata ctagcattag
    10201 cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat
    10261 ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac
    10321 gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa
    10381 attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt
    10441 tcagccaatg aagtaaagca agatgtaaca tctgaagtgg taataggtgt gctagattct
    10501 aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag
    10561 gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg
    10621 gaagagtcaa aagtagagat aacagagcca gctcaagcgg atgatatccg caaggttgtt
    10681 ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga
    10741 ggctacctag ctcagattgc agcggttgaa gattaccaaa aatatcctga tttttataac
    10801 catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt
    10861 tgggatgcta agaatggttt ctgagatgtt ggtttggaaa gtcgtaaatt agctgttagt
    10921 ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat
    10981 aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat
    11041 tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat
    11101 aaacatggtt atcgtgatcc gaaattggat aaagcgcgat tctttaagaa acaggctcta
    11161 cctcaatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag
    11221 gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc
    11281 gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg
    11341 caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac
    11401 actgcccagg atagattggc aaactttgat gtcattcttt tagacggttc tggtaaagaa
    11461 attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat
    11521 aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag tcttgcagaa
    11581 gttgaagttt tccgctttat agctacgaat ggtgaaacgg cgatacaagt ttctaagtca
    11641 gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga
    11701 gcttacattg cccgctactc cataacttgg gaagaagtta cagtagataa agatggaaac
    11761 caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc aggttttgtc
    11821 ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg
    11881 ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt agctcaaccc
    11941 caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc
    12001 ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag tactacctga
    12061 gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc aatgaaaatc
    12121 aaagagcaaa cta
//
LOCUS       DBERCA010000052        12014 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.052, whole genome shotgun sequence.
ACCESSION   DBERCA010000052 DBERCA010000000
VERSION     DBERCA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.052"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            17..830
                     /locus_tag="ACL719_001611"
                     /pseudo
     CDS             17..830
                     /locus_tag="ACL719_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            852..1591
                     /locus_tag="ACL719_001612"
                     /pseudo
     CDS             852..1591
                     /locus_tag="ACL719_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1604..2770
                     /locus_tag="ACL719_001613"
     CDS             1604..2770
                     /locus_tag="ACL719_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS0699548.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            3278..4771
                     /locus_tag="ACL719_001614"
     CDS             3278..4771
                     /locus_tag="ACL719_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS0699549.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4784..5905
                     /locus_tag="ACL719_001615"
     CDS             4784..5905
                     /locus_tag="ACL719_001615"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0699550.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNELVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            5965..6201
                     /locus_tag="ACL719_001616"
     CDS             5965..6201
                     /locus_tag="ACL719_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGS0699551.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            6198..6611
                     /locus_tag="ACL719_001617"
     CDS             6198..6611
                     /locus_tag="ACL719_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGS0699552.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            6729..7694
                     /gene="xerA"
                     /locus_tag="ACL719_001618"
     CDS             6729..7694
                     /gene="xerA"
                     /locus_tag="ACL719_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGS0699553.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            7724..8293
                     /locus_tag="ACL719_001619"
     CDS             7724..8293
                     /locus_tag="ACL719_001619"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0699554.1"
                     /translation="MHEESEIRASGYFYQCKPQDWSSEGKEIIRIQNLTKTSKGINYY
                     SGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKYVV
                     EKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQEQL
                     EELNLLVKSQFACEIAIKVWRNSLKFSII"
     gene            8323..11673
                     /locus_tag="ACL719_001620"
     CDS             8323..11673
                     /locus_tag="ACL719_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS0699555.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYCATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISDQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         3968 a         1955 c         2478 g         3613 t
ORIGIN      
        1 agagagtttt ctgacaatga atcaatccta cttttatcta aaaatgaaag aacacaaact
       61 caaggttcct tatacaggta aggagcgccg tgtacgtatt cttcttccta aagattatga
      121 gaaagataca gaccgttcct atcctgttgt atactttcat gacgggcaaa atgtttttaa
      181 tagcaaagag tctttcattg gacattcatg gaagattatc ccagctatca aacgaaatcc
      241 ggatatcagt cgcatgattg tcgttgctat tgacaatgat ggtatggggc ggatgaatga
      301 gtatgcggct tggaagttcc aagaatctcc tatcccaggg cagcagtttg gtggtaaggg
      361 tgtggagtat gctgagtttg tcatggaggt ggtcaagcct tttatcgatg agacctatcg
      421 tacaaaagca gactgccagc atacggctat gattggttcc tcactaggag gcaatattac
      481 ccagtttatc ggtttggaat accaagaaaa aattggttgc ttgggcgttt tttcatctgc
      541 aaactggctc caccaaccgc tatatcgagt gccagaaact atcgcctgac caacgcatct
      601 tcatctatgt aggaacagaa gaagcagatg atacagacaa gaccttgatg gatggcaata
      661 tcaaacaagc ctatatcgac tcgtcgcttt gctattacca tgatttgata gcagggggag
      721 tacatctgga taatcttgtg ctaaaagttc agtctggtgc catccatagt gaaatccctt
      781 ggtcagaaaa tctaccagat tgtctgagat tttttgcaga aaaatggtaa gttaagaaag
      841 gaaaaaacga aatgcatatt gaacatctta gccactggag tggtcatcct aaccgtgaaa
      901 tgtaccttaa ccgttatgga catggtggga ttccagttgt ggtctttgct tcatcaggtg
      961 gtagtcacaa cgaatactat gattttggca tgattgatgc ctgtgcttcc tttatcgagg
     1021 aaggccgtgt ccagttcttt accctatcta gtgtggatag tgagagctgg ttggctactt
     1081 ggaaaaatgc tcatgaccaa gcggaaatgc accgtgccta cgaacgttat gtgattgagg
     1141 aggccattct tttatcaagc acaagacagg ttagtttgat ggcatgatga cgacaggttg
     1201 ctctatggga gcctatcatg cactcaattt cttcctccag catccagatg tctttaccaa
     1261 agtgattgct ctcagtggtg tttacgacgc acgtttcttt gtcggtgatt actacaacga
     1321 tgatgctatt taccaaaact cgccagtaga ttatatttgg aaccaaaacg atggctggtt
     1381 tattgaccgt taccgtcagg cagagattgt gctgtgtacg gggcttggag cctgggaaca
     1441 agatggttta ccatcctttt acaagctcaa agaagccttt gaccagaaac aaattccagc
     1501 ctggtttgct gaatggggac atgatgtcgc ccacgactgg gaatggtggc gtaaacaaat
     1561 gccttatttc ctcggtaatc tctatttata aaaggagtta cctatgaatt accttgttat
     1621 ttctccctac tatccacaaa actttcaaca gtttaccatc gaactagcta ataaaggcat
     1681 cacagtcttg ggaattggtc aagagtctta cgagcaattg gatgagccct tgcgcaatag
     1741 cttgaccgag tattttcgtg ttgataatct tgagaacata gatgaagtca aacgtgcagt
     1801 tgcttttctc ttttataaac atggtccaat tgaccgcatc gagtctcaca atgaatactg
     1861 gcttgagcta gacgcaacac tcagagaaca attcaatgtt tttggtgcca aaccagagga
     1921 tctcaaaaag acgaaatata agtctgaaat gaagaaactt ttcaaaaaag caggtgttcc
     1981 tgtggtacct ggagctgtta tcaagacgga agcagatgtt gatcaagcag tgaaagaaat
     2041 cggtcttcca atgattgcca aacctgataa tggagtggga gcagccgcaa cctttaaact
     2101 tgagacagaa gacgatatca atcacttcaa gcaagaatgg gaccattcaa ccctttattt
     2161 ctttgaaaaa tttgtcactt ccagcgaaat ctgtaccttt gacgggctcg tggacaagga
     2221 tggaaagatt gtcttctcaa caacctttga ctacgcctat acaccgcttg acctcatgat
     2281 ttataagatg gacaattctt attatgtgct caaggatatg gatcctaaac tgcgcaagta
     2341 tggggaagca attgtcaaag aatttggtat gaaagaacgg tttttccata ttgagttctt
     2401 ccgtgagggg gacgattata ttaccatcga gtacaataac cgccctgcag gtggttttac
     2461 cattgatgtt tataactttg ctcattcctt ggacctttat cgtggctatg cagctattgt
     2521 cgcaggagag gagttcccgg cgtcagactt tgaaactcag tattgtttgg ctacttctcg
     2581 ccgtgcaaat gctcactatg tttattcaga agaggatttg cttgccaaat atagccagca
     2641 gttcaaggtt aaaaaagtca tgccagctgc cttcgcggaa cttcaaggag attacctgta
     2701 tatgctgaca actccgagtc gacaagaaat ggatcagatg attgctgatt tcggacaacg
     2761 tcaagaataa gaactatcgg attaaggaaa ttcactccct taatcctttt gttttgtctg
     2821 gtaaaaaata agagcatcct aacaaggtag ctatcataaa acttgttcga taactatttg
     2881 aagcaggatt aggtggctag aaattgaatt ttaacatttc aatttagtca tagtattgtg
     2941 ttttcgtatc cttaaatcag ctaaaaggat ccatgacgac acctatacga tatagttttc
     3001 aggataccaa accagtctat taatactcaa tgaaaatcaa agagcaaact aggaagctag
     3061 ccgcaggttg ctcaaaacac tgttttgagg ttgtggatag aactgacaga gtcagtatca
     3121 tatacctacg gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaata
     3181 ttcaggtgac gcatagatat agttaattga agctttgttt gaaatctgat aaaataatga
     3241 tattactaag ttttaaaaac taaagaaaag ggaagatatg attacaggcg aattaaaaaa
     3301 taaaatcgac cagctgtggg aaattctttg gacagaagga aacgcaaatc ctttaacaaa
     3361 tattgaacag ttgacttatc tcttatttat gaaagatttg gatagtgtcg agcttggacg
     3421 tgaaagtgat gctgaatttc tagggattcc ttatgaggga gtttttccaa aagataaacc
     3481 tgaataccgt tggtcaactt ttaaaaatat aggagatgct caggaagttt atcgtttaat
     3541 gactcaggag atttttccgt ttattaaaaa tctcaagggg gatacagatg atacagcctt
     3601 ttcacgatat atgcgagaag ctatttttca aataaataaa cctgctacgc ttcaaaaggc
     3661 aatttctatc ttagatgttt ttccaactag gggattagat gtagattttg ataatgacaa
     3721 acaaagtatt actgatatcg gagatatcta tgaatatctg ttatcaaaat tgtcgaccgc
     3781 aggtaaaaat ggacagttcc gtacacctcg tcacatcatc gatatgatgg ttgagttgat
     3841 gcaaccgact atcaaagata tcatctcaga tcccgctatg ggttctgctg gcttcttagt
     3901 atctgctagc cgttacttaa agcgtaagaa agatgaatgg gaaaccaata cagataatat
     3961 caatcatttt cataatcaga tgtttcatgg aaatgatacg gatacgacta tgttgagact
     4021 tggggcgatg aacatgatgc tacatggagt agaaaatcca caaatcagtt accttgactc
     4081 gctgtctcaa gataatgaag aagccgataa atatactttg gttttagcaa atcctccttt
     4141 taagggctca cttgactaca attcaacctc taatgacctt cttgcaaccg taaaaaccaa
     4201 aaaaacagaa ttactctttc tttctctttt cttgcgaact ttaaaaccag gtggacgagc
     4261 agcagttatc gtacctgatg gtgtcctttt tggttcgtct aaagctcata aaggaattcg
     4321 tcaggaaatt gtagagaatc ataagcttga tgctgtaatc tcaatgccta gtggtgtgtt
     4381 caagccttat gctggagttt caactgccat tctcatcttt acaaaaactg gtaatggtgg
     4441 tactgacaaa gtctggtttt acgatatgaa agcggatggt ttaagtttgg atgataagcg
     4501 acaaccgatt agcgacaatg atattccaga tattatcgaa cgctttcatc atcttgaaaa
     4561 agaagcagaa cgtcagagaa cggatcaatc tttctttgtt ccagttgctg agataaagga
     4621 aaatgattat gatttgtcta tcaataaata taaagagatt gagtatgaaa aagttgagta
     4681 tgaaccaaca gaagtcatat taaagaaaat caatgattta gaaaaagaaa ttcaagctgg
     4741 cttggctgaa ttggaaaaat tactcaagta gggaggtggc tgtatgaaaa aagtgaagtt
     4801 gggggaagtt tgtgagattt tatctggtta cgcttttaaa tcatcacaat ttaatgataa
     4861 caaaattggt ctccccttaa tacgaattcg agatgtagaa agaggttttt ctgataccta
     4921 ctttgaagga acttatcccg aagaatatct tattaaaaat ggagacttat tgattacaat
     4981 ggatggcagt tttatattga aaaaatggga gggggattta gcgctactta atcaacgtgt
     5041 ctgtaaaatt aaaattacgg ataaaagtgt agatgaaggt tatatatctt ggttgattcc
     5101 taaattttta aaagaaattg aagataagac cccttttgtt acagtgaaac atctttcagt
     5161 agctaaaata aaagatatct catttgtatt gcctaataaa ctagaacaaa agctaattgc
     5221 aaaaaaacta aatacgataa gtcaaattta tgattttcgt aaaattcaat cagaaaaatt
     5281 taacgagctc gtcaaatccc gatttaacga gatgtttggg gaaaataaaa tatttgaaag
     5341 cattgataac ttatttgata ttatagatgg tgataggggc aaaaattatc ctaaatcaga
     5401 tgagttgttt agtgaggagt actgtttatt tttaaataca aagaatgtta ctaaaaacgg
     5461 attttcattc gatacaaagc aatttatcac taaaacaaag gataaattac ttcgaaaagg
     5521 caaacttgag cgttatgata tagtcttgac aacaagaggt actgttggaa atgtagcgta
     5581 ctacgatgaa ttaataaaat ataaacattt acgtataaat tcaggtatgg taatattacg
     5641 tcccaagaca ccaaatctaa atcagaaatt tattatccat gttttaagga ataataatta
     5701 tagtcgagtg atatcaggaa gtgctcagcc tcagttacca attacaaaat taaaaaaaat
     5761 acttctcccc ctccccccac tagccctcca aaatgagttc gcagactttg tagtccaggt
     5821 cgacaaatca caattggcaa tccaaaaatc tctggaagaa cttgaaactt tgaagaaatc
     5881 tctgatgcag gagtattttg gctgatattc tgccattgta attacggtaa tgatttgtta
     5941 taatacttca aaggaggaaa tcagatggta gtaaaaacaa gaaaacaagg aaattcaatc
     6001 accattacga ttccaagtga atttaatatt ccaagtggtg ttaaatacga agcgaaattg
     6061 ttaccaagtg gtgagattat ctttactcct gaagaattgg ggcagcaggt ttcttatgta
     6121 tctgatgatg cctttgactt aaatttagat aaaatatttg acgaatacga cgatgttttc
     6181 aaagctttgg tggaaaaatg acaatctatc tgacagaaaa gcaaattgaa aaaataaatg
     6241 ctttagcaat tcaacggtat tctccaaatg agaaaattca aacagttagt tcttctgcct
     6301 taaatatgat tgtgaactta ccagaacaat ttgtctttgg gaagtctctt tatccaacaa
     6361 tttttgataa agcaacgata ctatttgtcc aattgataaa gaagcatgtt tttgctaatg
     6421 ctaataaaag aactgctttc ttcgttttgg tcaaattttt acaattaaac ggctatcgtt
     6481 tttctgtaac ggtagaagaa gcagtaaaaa tgtgtgtaac catcgcagta gaagctttaa
     6541 ctgatgaaaa aatgacaagc tactccaaat gggtttctga acattctgtt agagaaaagg
     6601 tcaaaaagta acctagtatg ctggatttga atgagcacaa gaaaataaat gaacagacaa
     6661 tattagaatt ctgtaatgca gaaactgata ttgtctcttt ttattgatga ataagaaagt
     6721 gagaaattat ggaatcaaaa gttacaatta tcatgcaaga aatgttacct cttttaaata
     6781 atgaacaatt actagcgttg agagagagtt tagaacatca tctagtagac ggaaaaaagc
     6841 agcagaagta ttcgaataat aacctgttgc aactatttat taccgccaag caggtagagg
     6901 gctgtagctc aaaaacaatt cgttattacc agaggacgat tgaaaacttg tttaatgcta
     6961 ttaaagagtc tgtgacacaa ctcacaacag atgatttaag gagttattta gcaaattacc
     7021 agtctgaaaa ggattgtagt aaggcaaatt tagacaatat taggcgtata ttgtcttctt
     7081 tttttgcttg gcttgagcaa gaggaatata tcattaaaaa tcccattcga cggataaaga
     7141 aaattaagac tgagcaaaat gtgaaggaaa cttatactga tgaacatttg gaaattatgc
     7201 gtgataactg tgaaaatttg agagatttgg caataataga cctactagca tcgacaggta
     7261 tgcgtgtagg ggagcttgta cagttgaatc gttcagatat tgattttgaa aacagagagt
     7321 gtgttgtctt tggtaaagga aagaaggaga gaccagtata ttttgatgct cggacgaaaa
     7381 ttcatttaag aaattatctt aacgacagaa aagatagtca ccctgctctt tttgtaacgc
     7441 tagttggaaa agcccagaga cttggaattg ctggtgtaga gattcgctta agaaagttag
     7501 gagacaaact cggcatacaa aaggttcacc cacataagtt cagaagaact ttagcgacta
     7561 aggcaattga taaaggtatg cctatcgaac aagtccaaaa actgctaggt catagcaaga
     7621 ttgacacaac cctggcctat gccatggtca atcaaaataa tgtcaagcat tcacaccaaa
     7681 aattcatctc ttaaaagcaa atcccgatat tccgaggagg tggttgcatg aagaaagtga
     7741 aattagggca agtggctact tttatcaatg taaacctcaa gattggtcat ctgaagggaa
     7801 agagattatc agaatacaga atctgactaa aacttcaaaa gggataaatt attattcggg
     7861 tacgattgat aaaaaataca ttgttgaagc tggtgatatt cttatttcgt ggtcgggtac
     7921 attaggggtt tttcagtggt gtggaagatc cgcagtgtta aatcagcaca tttttaaagt
     7981 ggtttttgat aagatagata tagataaatc gtattttaaa tatgttgttg aaaaaggttt
     8041 acaagatgct gtaaaacata ctcatggttc cacaatgaaa catttaacta aaaaatattt
     8101 tgataatatt atggtttcat atacaaattt gggagagcaa caaagaattg caagtgagct
     8161 ggatttgtta agcaagctga ttttaaggag acaagaacaa cttgaagagc ttaatctact
     8221 tgtaaaatca caatttgctt gtgagatagc tataaaagtg tggagaaata gcttgaaatt
     8281 tagtataata tagctaaact atttgtttaa agtgagaaaa aaatgggaaa ttttagcttt
     8341 cttttaaaaa atgacgaata tgaatctttt tcaaaacctt gcattgaagc tgaaaatatg
     8401 attgctacat caactgtggc tactgccttt atggcgcgtc gtgctttgga gcaggctgtc
     8461 cattggatat atagtcacga ttcatattta gaagctccct attgtgctac tctatcttct
     8521 ttagtatggg atgatgattt tagggatatc gtagattctg aactccacaa gcagatagtt
     8581 ctgttgattc ggtggggaaa ccatgctgct catggtggtg aaattaagga acgagaagcg
     8641 attttagctt tacatcattt gtatcagttt gttaatttta tcgattattg ttacagcaat
     8701 gaatttgtgg agcgttattt tgatgagaag tgtttaccac tttcagcaaa catcaaatac
     8761 cgagaaactc cacaatctat gataaagtta caagacagtt taccagaact gcctgatttt
     8821 catgaacaga tggctgctca gtccgtagaa gttcaagaga cttatactga aaaacgtgag
     8881 actgcagcgc aacggcaaga tgtgcctttc catattgaac aattatctga ggcagagaca
     8941 agaaagctct ttattgatat cgatctccgt ttagcaggat ggatatttga agaaaactgt
     9001 cgtgttgaga tagccgttga tggtctcaag cacggttcag gaattggtta ctgtgactat
     9061 gtactttatg gtaaaaatgg gaaaatttta gcgattgtgg aggctaaaaa agcctctgtc
     9121 aatccagaag taggggaagt acaggtcaaa gaatatgctg aagctttgga gaaacatatc
     9181 ggctatcagc caatttgctt tattacaaat gggttgaagc actatatact tgatggtccg
     9241 aaccgccgcc agattgcagg cttttactct caagaagaat tgcaattagt gatggataga
     9301 cgtcatcttc aaaaaccgct tgaggatatt tctagtaaaa ttagggacga tatttccggg
     9361 cgtcactacc aaaaacatgc cattgcaagc gtttgtgaag ctttctctaa tcatcgtaga
     9421 caggcacttt tggttatggc aactggggcg gggaaaactc gtacagcagt ttctctagtt
     9481 gatatcttat cacgtcataa ctgggtaaaa aacgttctct tcttagccga tagaacttcc
     9541 ttggttaagc aagcctatga ttcgtttaga aaattactcc cagatctttc cgtttgtaac
     9601 ttcttagaag ataaagaagg agctcaatca agtcgtatgg tcttttcaac ttatccgacc
     9661 atgattggag cgattagtga tcaagaagaa gtaaatcaac gcccttttac tgttgggcat
     9721 tttgacctta tcataattga cgaatctcac cgttctattt atcagaaata caagtccatt
     9781 tttgattatt ttgatgcaag gattgtaggc ttaacagcta ctccgcgtca agatttagat
     9841 aaaaacacct atggattctt taatttggag aatggggttc caacatatgc atatgatttg
     9901 gaagaggctg ttaaagacgg atatttagta gcctatcatt ctatcgaaac caaactgaaa
     9961 ctacctacgg atggtctaca ttatgatgat ttgtccgaag aagaaaagga acattttgat
    10021 agcaaatttg aagacgatag ctgtgaaaaa gatattgatg ggagtgtgtt taattccttt
    10081 attttcaata aaagtacagt agaaattgtt ttaaatgaac tcatgacaag aggaattcag
    10141 acagcctcgg gtgatgaaat tggtaaaact attatttttg ctaaaaatca tgaccatgcg
    10201 gaatatatca gaggtatttt taacaaccgc tatcctgaaa aagggagcga ctatgctcag
    10261 gtgattgatt atagtattaa gcattatcag accttgattg atgattttaa aattaaggag
    10321 aagtatcctc aaattgcgat ttctgtcgat atgttagata caggtattga tgtaccagag
    10381 gttgttaatt tagtcttctt caagaaagta cgctctaaaa ctaagttttg gcagatgatt
    10441 ggtcgaggaa cccgtctatg taaagattta tttggacctg agcaggataa ggaaaacttc
    10501 ttggtatttg attatgggga caattttgat tattttcgtg cagatccaag agatggagag
    10561 ggtcgtcaca ttgtttcgtt gactcagcgt ttatttaata tcaaagtgga cttgattcga
    10621 gaacttcagg gacttcaata ccaagaagat cagtttgcga gagcataccg tcagcagctt
    10681 gtctcggaac ttcaaggtcg tatagagagc ttaaatgagt tggacttcag ggttcgtatg
    10741 gttttagata cagtttatag ctataggaaa ttggaaagtt ggcagaatct aactgctgtt
    10801 acaagtgaaa ccattcaaaa aaatctctct ccgcttttat ttgatgaaga taaagaagat
    10861 gagatggcca ggagatttga tttgtggttg cttcatattc agttggggca actgacagct
    10921 aaatcttcca ctgttcatat ttcccaagtg atgaagacgg ctagagctct ttctgctatt
    10981 ggcaatatcc cgcaggtttt tgagcaggct gaaattatca ggaaagtaca ggagcctgaa
    11041 ttttggaaag aagttaactt gtctgatttg gaaaaaattc gtcttgctat tcgagattta
    11101 ttacagtttt tggataaaac agaccgtaaa ccctactatg ttaactttga agatcgtata
    11161 ctctccactg ttcacgagac cacagcattt ttgcaggtca acgatcttcg gtcttacaat
    11221 gaaaaagttg agcattattt gaaaactcat ctggatgagg agtccatttc taagctatac
    11281 cataataaaa agttgacatc tgatgatatg cttgcacttg aaaaattgct ctgggaaaaa
    11341 ttaggtagta aagcagacta ccaaagtcat tatgaaaata aggcaattcc gagattggtt
    11401 cgtgagatta ttggcttaga tagagagtct gccaatcgta ttttttctaa atttttgtcg
    11461 gatgagaatc ttaatgccag gcagatttca tttgtaaaat tgattgtaga ctacattgta
    11521 gaaaatggtt ttttagagac gaaagtgtta acgcaagagc cgtttaaatc ttatggttct
    11581 gttcaactac tcttccaaca ccaactacca gtacttcgta atattgttca aatcattgaa
    11641 cttatcaata atcgagctgg agaagcggct taaattctaa agtgattgcc atgctgagac
    11701 tcatttaaaa ttaaaaagag tagaaattta tgctatatat gagaagtttt attaggaaga
    11761 atgtcatcgt tttcctagaa tacagtatca gttgttaagt ggttgataaa tttcaaagta
    11821 gatacttgta ccacgatgtt tgttgatcga gttattaaca aaagagctac tttgatttta
    11881 aagaaataga aaacaaaaag ctgagcaaga attcaattgc aggagaaaat gaaataatac
    11941 tcaatgaaaa tcaaagagca aactaggaaa ctagctgcag gctgctcaaa acactgtttt
    12001 gaggttgtag ataa
//
LOCUS       DBERCA010000053        11968 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.053, whole genome shotgun sequence.
ACCESSION   DBERCA010000053 DBERCA010000000
VERSION     DBERCA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.053"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            213..482
                     /gene="blpJ"
                     /locus_tag="ACL719_001621"
     CDS             213..482
                     /gene="blpJ"
                     /locus_tag="ACL719_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGS0699556.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            551..781
                     /gene="blpU"
                     /locus_tag="ACL719_001622"
     CDS             551..781
                     /gene="blpU"
                     /locus_tag="ACL719_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGS0699557.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            784..903
                     /locus_tag="ACL719_001623"
     CDS             784..903
                     /locus_tag="ACL719_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS0699558.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDNLIRKIFSKFPKKDTDSTP"
     gene            1200..1361
                     /locus_tag="ACL719_001624"
     CDS             1200..1361
                     /locus_tag="ACL719_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699559.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIVYMIAVMIYF
                     SLIKKKEKK"
     gene            1423..1742
                     /locus_tag="ACL719_001625"
                     /pseudo
     CDS             1423..1742
                     /locus_tag="ACL719_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            2371..2754
                     /locus_tag="ACL719_001626"
     CDS             2371..2754
                     /locus_tag="ACL719_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGS0699560.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2806..3495
                     /locus_tag="ACL719_001627"
     CDS             2806..3495
                     /locus_tag="ACL719_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0699561.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRELLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3537..3770
                     /gene="blpZ"
                     /locus_tag="ACL719_001628"
     CDS             3537..3770
                     /gene="blpZ"
                     /locus_tag="ACL719_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGS0699562.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3921..4532
                     /locus_tag="ACL719_001629"
     CDS             3921..4532
                     /locus_tag="ACL719_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0699563.1"
                     /translation="MEFFDKFHALCFGFLVLIIVIIVPYTINHGDFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            4694..5488
                     /gene="ccrZ"
                     /locus_tag="ACL719_001630"
     CDS             4694..5488
                     /gene="ccrZ"
                     /locus_tag="ACL719_001630"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGS0699564.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5485..6120
                     /gene="trmB"
                     /locus_tag="ACL719_001631"
     CDS             5485..6120
                     /gene="trmB"
                     /locus_tag="ACL719_001631"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGS0699565.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6246..6725
                     /gene="rimP"
                     /locus_tag="ACL719_001632"
     CDS             6246..6725
                     /gene="rimP"
                     /locus_tag="ACL719_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGS0699566.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6769..7905
                     /gene="nusA"
                     /locus_tag="ACL719_001633"
     CDS             6769..7905
                     /gene="nusA"
                     /locus_tag="ACL719_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGS0699567.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7927..8220
                     /locus_tag="ACL719_001634"
     CDS             7927..8220
                     /locus_tag="ACL719_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGS0699568.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8213..8512
                     /locus_tag="ACL719_001635"
     CDS             8213..8512
                     /locus_tag="ACL719_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGS0699569.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIEDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8529..11294
                     /gene="infB"
                     /locus_tag="ACL719_001636"
     CDS             8529..11294
                     /gene="infB"
                     /locus_tag="ACL719_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGS0699570.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAEPVAPKTEKVAAKPQSRNFK
                     AEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQ
                     QGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKE
                     ALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRD
                     DYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFH
                     ELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYG
                     IEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRV
                     ATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDITILVVAADDG
                     VMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVE
                     ISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGT
                     LNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEK
                     SARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSA
                     SLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVE
                     IRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKV
                     ARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAY
                     VMEEIKR"
     gene            11545..11895
                     /gene="rbfA"
                     /locus_tag="ACL719_001637"
     CDS             11545..11895
                     /gene="rbfA"
                     /locus_tag="ACL719_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGS0699571.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3657 a         2087 c         2695 g         3529 t
ORIGIN      
        1 ctttttattg caaggagaat aacgaaatta agatatttta gatactcaag attattgaaa
       61 tcttagacta aaaaataggt actgctactt ttaagaagat agtagacgta tatacttttt
      121 taagaaaatc aaaaagatac tataaaaaat ctatttgttt attgaattta agactttggt
      181 aacaaattga aaataaaaag gaggtattca tcatgaatac aaaaatgttg tcacagttag
      241 aggttatgga tactgaaatg cttgcgaaag ttgaaggggg gtatagctcc actgattgtc
      301 aaaatgcact gattacagga gtcactacgg gaattataac tggtggaact ggagcaggtt
      361 tggccacttt aggagtagct ggacttgctg gtgcatttgt tggagcacac attggtgcaa
      421 ttgggggtgg attaacatgc ttaggaggta tggttggtga taaattggga ctaagttggt
      481 aagacttata gtaaaattat gaggattcta ttctgattta ttttagtttt ttaaaaaagg
      541 agtatttgtc atggatacaa aaatgatgtc acaattttct gttatggata ctgaaatgct
      601 tgcttgcgtt gaaggtggcg gatgcaattg gggagatttt gccaaagcag gtgttggagg
      661 agcagcagca agaggtcttc aactaggcat taaaacagga acatggcaag gtgcagcaac
      721 tggtgctgcg ggtggagcta tacttggagg tgtagcctat gcagcaacat gttggtggta
      781 attatggatt ttaaaagttt tattattggt ttagtagttg gtatatttgg tccttatatg
      841 gataatttaa ttagaaaaat attttcaaaa tttcccaaaa aggatacaga tagtaccccc
      901 taaaaaataa atttctcccc tgtatttgct agcttaattt acaggggagt ttctttaata
      961 ttgtttcgcc tgtcaaaatg atatgtgata gcaggatgaa aagtctacaa acaagttggt
     1021 atcaatacaa tataaagagg aaagtatgtt tttcatgctt gcttttttaa tttttacaat
     1081 tcaagaggtt ttgatgacca tttatgattt gagtgatcca aggtcaaaat aagtgctata
     1141 ctagcagtgt aaatgttctt gctcaactaa gataaatcgc acatttagga ggaaatctta
     1201 tgaagaaaaa aatactgatt attttcgttc tgtatctaat catgtccatc tttctatatc
     1261 cgcttaggga gagtgcttgg tatcagctat tttataccat agtctatatg attgcggtta
     1321 tgatctattt ttctttaatt aaaaagaaag aaaagaaatg aaagattttc ttgattaaaa
     1381 agaatcatat tctttgtgag attgttcctg ggtcagttgc ctttgcttgt ttctacttat
     1441 ctatttctat ttcgtcagtt ttccaatttc ttttagtggt tattaacttg acttctattt
     1501 tggttactgt ttatcttact agggaaatga gattaagaga gtttgaagat gatgatttgg
     1561 ttagtcctag aaccaatcaa ctcatgttta tcggcttgac aggctttatg tctattattt
     1621 gtttgtatag agggatcaca gcaggagaat cctaccaaca aataattgcc tacattggcg
     1681 ctattctctg cttgaccatc atgcttctgc tcatttgggg attgaagtat tataaaaagt
     1741 agaggctaat gcagtttttg caatttaaga tgtcttgatg acaattcaag atttggatga
     1801 aaaactttga caaatagtgc tatactgaaa ttgtcaaagt tgcaacgggt gctgctacat
     1861 tatgttaaaa atcgctcttg attgataagc tggatttgtt aggaaaggag taaataatta
     1921 tggataagaa aaagatagtt tcgactataa tatgtatagt atttcttgta gtgtcagtag
     1981 ataatttttt tagagactta accccgttac tgttcatttt aaatattatt ggtctgtctt
     2041 gcttttcagt gctaacatac actaacataa aagagatact cttaaatata agtaaatgat
     2101 gatttggtta gtcctagaac caatcaactt atgtttatcg gcttggcaga ttttatgttt
     2161 gtcatttgtt tgtatagagg tatttcagaa acagaattct atcaacaact aatcgtttat
     2221 ataggtgtat tctctgcttg tttatcacgc ttctgctcat gtggggcttg aagtattata
     2281 aaaagtagct ttctatcctt ttctgacagg atatttgagt agaaggctct attaaattgt
     2341 tagtgaaata aatgaatgga ggtatttaat atgaaataca gattattttt tgttattttc
     2401 ttgagtagtg tgttggatat tcttttaggg acatttttac aaatctctat cgtatctatt
     2461 gggtggcttg ttctttacag tggtttgttt gaagcaggag ttttccttct tgctaataaa
     2521 ggggtggcgg taaaaatcaa ggaagtagat attcgaaatc gctttaaatt tatttttgga
     2581 aaaaccttat ggtttcaaat tcttttgctc atctttttga taatcaaact ttatcttggt
     2641 ttggatgcga ggttgatttt attctatgga catattttca ttgtctttaa tgccttaatg
     2701 tatctattat ctagtagtca ggttagcctt aaaaaaaaca aactgtcttc ttaatcttac
     2761 agcagtagag cctgacaagg tgaggtcata gtagggagga tttctatgaa aaagtatcaa
     2821 cttctattca aaataagtgc agtcttctct tacttatttt tcgtatttgg tctttctcag
     2881 ctgacgctta tcgtccaaaa ttattggcaa ttttcttccc agattggtaa tttcgtctgg
     2941 attcaaaata tcttgagttt actttttatt ggagtcatga ttgtggttct tgttaagaca
     3001 ggccatggtt atctcttccg cattccaaga aaaaaatggc tttggtattc gattttgaca
     3061 gtattagtgg tagtgttcca gatctctttt aacgttcaga cagctaaaca tgttcagtca
     3121 actgcggaag gttgggctgt attgattggt tatagtggga ctaactttgc agagctaggt
     3181 atttatatag ccctgttctt tctggttcca ctgatggaag agttaatcta tagagaatta
     3241 ctgcaacatg ctttctttaa gcattcgcga tttggtcttg atttgcttct tccttctatt
     3301 ttatttgctc tccctcattt ttcaagcctg cctagtctgt tagatatctt cgtctttgca
     3361 acagttggaa tcatctttgc tggtttgacc cgctatacca agagcattta tccatcctat
     3421 gcggtgcatg tgatcaataa tattgtagcg accttcccgt ttttgctcac ttttctacat
     3481 agggtcttgg ggtaaaaaaa caaaaggctt gcttttcagc catagaggag gtcatcatgt
     3541 ataaacactt atttttccta gattccaaaa ctttagatcg gttgacaccc tatattctag
     3601 tcttggcttc tgacaccatt gcttttaatg tttttgtgct aacctttgta tctgcggtgg
     3661 tttttaattt cctaaattcc atgctagctt taatggctat attcataggg gctggctatg
     3721 tggtcggatt ttggttacta atactcaatg aaaatcaaag agcaaactag gaaactagcc
     3781 gcaggctgta cttgagtacg gcaaggcgac gttgacgtgg tttgaatttg attttcgaag
     3841 agtataaaat ggtttgtttt ggaaaggcta gaactaaaga atgacgtgta ggaagtctgt
     3901 ttgttgagga ggataatttt atggagtttt ttgataaatt tcatgccttg tgttttggat
     3961 ttttagtact aataattgtc attatagttc cttatacgat taaccatggg gatttttttc
     4021 aaaatgaatc tgcattgatt cttgtaagtc ttcttgtaac ctcgctgagt gttgcttatg
     4081 ctagaaagtt tgaaatgatt tcttttggga tgttaagcaa gaaacaactt ttgcttttca
     4141 ttgcaatctt tcttctaagt gtacttgaga cgctggttta tattcatttc ttcgctgttt
     4201 cttctggctc aggggtccaa cacttggcgg aagtcagcag aggaatttcc ctgtctttga
     4261 ttttgactac ctcagttttt ggccccatcc aggaggaact cattttcaga ggacttcttc
     4321 aaggtgcggt ttttgacaat tcttggttag ggcttgtgct aacttcctct ctcttttctt
     4381 tcatgcatgg accttctaat gtcccttcgt ttatttttta tctacttggg ggcttgttgc
     4441 tgggccttgc ttataaaaag agccaaaact tatgggtttc tactctagtc catatgcttt
     4501 acaacagttg gccactctta tattatttat aaaaatcatg aaaaggtaag taggagattg
     4561 tgcttacctt tttctttcta taatgataac ccaaaccact ccagaggctt ttctttgaga
     4621 atcgggttag agtggttgac gaataggcca aaaactagta gaatagtaag gaaactttat
     4681 acggaggaaa gaaatggatt tgggtgataa tgagctaaca ctgactccca tacctgggaa
     4741 aagtggcaag gcttatatgg gtagctatcc tgatgggaag cgcatctttg taaaaatgaa
     4801 cacctctcca atcctacctg gtctagctag agaacaaatt gctccacaat tattatggag
     4861 tcgtcgtttg gcagatgggc gtgatatgtg tgctcaagaa tggttgacag gcaagatatt
     4921 gaccccctat gatatgaatc gtaagcaaat cgtcaatatt ttaacccgtc ttcatcgctc
     4981 acgtccgttg atgacacaat tgagtcgttt gggctatgcc atggaaacac ctgtagattt
     5041 actacagtct tggcaagaaa cggctccaga tgctttgcgt aaaaatcatt ttatcagtga
     5101 agtgatggct gatttacgtc agactattcc aggatttaga gaggaccatg cgaccattgt
     5161 ccatggagat gtacgacata gtaattggat tgagacagat agtggcttga tttatttagt
     5221 agattgggat tcggttcgct tgaccgatcg catgtttgat gtggcccata tgctctgcca
     5281 ttatatttca gaacatcagt ggaaggaatg gttgacctac tacggttaca agtacaatca
     5341 aacggtatta agtaaattgt attggtatgg tcaattgtct tatttgagtc agatttccaa
     5401 gtattatatg aaccaagatt tagaaaatgt caatcgggag attcatggtt tgcgtcattt
     5461 ccgagacaag tatggaaaga gaagatgaga gttagaaatc gtaaaggggc aacagaatta
     5521 ctagaggcaa atccccagta tgtggtcctc aatcccttgg aagccaaggc aaaatggcgg
     5581 gacttgtttg gcaatgataa tcccattcat gtggaagttg gaagtggaaa gggtgccttt
     5641 gtttcaggta tggccaagca aaaccctgac atcaactata tcgggattga tattcaaaag
     5701 tctgttttga gctacgcttt ggacaaggtg cttgaagttg gagtgcctaa catcaagctc
     5761 ttgtgggtag atggttctga cttgactgac tactttgaag acggtgagat tgatcgcttg
     5821 tatctgaact tttcagatcc atggccgaaa aaacgccatg aaaagcgtcg tttgacctac
     5881 aagacctttt tggatacctt caaacgtatc ttgcctgaaa atggagaaat tcatttcaag
     5941 acggataacc gtggcttgtt tgagtacagt ttagtgagct tttctcaata tggcatgaaa
     6001 ctcaatggtg tctggctaga tttgcatgcc agtgattttg aaggcaatgt catgacagaa
     6061 tacgagcaaa aattctcaaa caaggggcaa gttatctacc gagttgaggc agaattttaa
     6121 gagatagcct aaaattaggc tgtacaagtg cttttgcttt acataagttg gcaaacgtgc
     6181 tatactgata gtaagaatat gaaaagtgag gcggggaaat atcttcgcct cttgcttatg
     6241 aggaggtgga cgcaatcgca acaatcgtag aattagtcag agaagttgta gaacctgtca
     6301 tagaagctcc ttttgaactc gtggatatcg agtatggaaa gattggcagt gacatgattc
     6361 tcagtatttt tgtagataaa cccgaaggaa ttaccttgaa cgacacggca gacttgacag
     6421 aaatgatcag tcctgtccta gacaccatca agccagatcc cttcccagaa caatatttcc
     6481 tagaaattac cagtccaggt ttggaacgtc ctttgaaaac caaggatgcc gtcgctggag
     6541 cggttggaaa atacatccat gtcgggctct accaagccat cgataagcaa aaggtctttg
     6601 aaggaacctt gttggccttc gaagaggacg agttgactat ggaatatatg gacaagacgc
     6661 gtaagaaaac cgtccaaatt ccatacagtt tagtatcaaa agcacgttta gcagttaaat
     6721 tatagaaaaa gaaaggatag cttttgagga ttcaaaagtg aagaaaacat gagtaaagaa
     6781 atgctagagg ccttccgcat tttggaagaa gacaagggaa tcaaaaaaga agatatcatc
     6841 gacgcagtag tagagtcgct tcgttccgct tatcgcagac gctatggtca gtcagacagc
     6901 gtagctattg acttcaacga aaaaacaggt gactttacag tttatactgt ccgtgaagtt
     6961 gttgatgaag tatttgatag ccgtttggaa atcagcttga aagatgctct tgccattaat
     7021 tcagcttatg aacttggaga caaaatcaag tttgaagaag caccagctga gtttggtcgt
     7081 gtagcagccc aatctgccaa acaaaccatc atggaaaaaa tgcgcaagca aacacgtgcc
     7141 atcacttaca atacttacaa agaacatgag caagaaatca tgtctggtac agtagaacgc
     7201 tttgacaacc gctttatcta tgtcaacctt ggtagcatcg aagcccaatt gtcaaaacaa
     7261 gaccaaattc ctggagaagt ttttgcttct catgatcgta tcgaagttta tgtttacaag
     7321 gttgaagaca accctcgtgg tgtgaacgtc tttgttagcc gtagccatcc agaaatgatc
     7381 aaacgtttga tggagcaaga aattccagaa gtttatgatg gaactgttga aatcatgagc
     7441 gtggctcgtg aagcaggtga ccgtacgaag gttgctgttc gtagccacaa tccaaacgtg
     7501 gatgctatcg gtacaatcgt tggacgtggt ggtgctaata tcaagaagat tactagcaaa
     7561 ttccacccag ctcgttacga tgctaaaaat gaccgcatgg taccaatcga agaaaatatc
     7621 gatgttatcg agtgggtagc agatccagct gaatttatct acaatgccat cgctcctgct
     7681 gaggttgacc aagttatctt tgatgaaaac gacagcaaac gtgccttggt ggttgttcca
     7741 gataacaagc tttctcttgc cattggtcgt cgtggacaaa acgtgcgctt ggcggctcac
     7801 ttgactggtt accgtatcga tatcaagtct gctagcgaat ttgaagccat ggaagacgct
     7861 gcttcagtag agttggaagt agaaaacgat actgtagaag aataaaagct gctagaggag
     7921 ggaaagatga aaacaagaaa aatccctttg cgcaagtctg ttgtgtctaa cgaagtgatt
     7981 gataagcgtg atttgctccg cattgtcaag aacaaggaag gacaagtctt tattgatcct
     8041 acgggcaagg ccaatggccg cggcgcttat atcaaactag acaatgcaga agccctagag
     8101 gcgaaaaaga agaaggtctt taaccgcagc tttagcatgg aagtggaaga aagcttttat
     8161 gacgagttga tcgcttatgt ggatcacaaa gtgaaaagaa gagagttggg acttgaataa
     8221 gcaaaagata agtaatctct tggggcttgc tcagcgagca gggcgcatca tatcgggtga
     8281 agaattggtg gtcaaggcca ttcaagacgg caaggccaag ttggtctttc tagctcatga
     8341 tgctggaccc aatctgacca agaagattga agataaaagt cattattatc aagtagaaat
     8401 tgtaaccgtg ttttcaacac tggaattaag catagcagtc gggaaatcga gaaaggtttt
     8461 ggctgtaaca gatgctggat ttacaaagaa aatgaggtct cttatggaat agaagaggag
     8521 gacatgattt gtctaagaaa agattgtacg aaatcgcaaa agaacttgga aaagaaagta
     8581 aagaagttgt agcgcgtgca aaagagttgg gcttggatgt gaaaagccac tcatcaagtg
     8641 tggaagaagc tgtcgctgca aaaattgctg ccagctttaa gcctgcagct gctccgaaag
     8701 tagaagcaaa acctgcagca ccaaaagtaa gtgcagaaaa gaaagccgaa aaatctgagc
     8761 cagctaaacc agctgagcca gtcgctccga aaacagaaaa agtagcggct aaaccgcaaa
     8821 gtcgtaattt caaggctgag cgtgaagcac gtgctaaaga gcaggcagag cgacgcaagc
     8881 aaaataaggg caataaccgt gaccaacaac aaaacggaaa ccgtcagaaa aacgacggcc
     8941 gtaatggtgg aaaacaaggt caaagcaacc gcgacaatcg tcgctttaat gaccaagcta
     9001 agaagcagca aggtcagcaa aaacgtagaa atgagcgccg tcagcaagag gataaacgtt
     9061 caaatcaagc ggctccacgt attgacttta aagcccgtgc agcagcccta aaagcagagc
     9121 aaaatgcaga gtacgctcgt tcaagtgagg aacgcttcaa gcagtatcag gctgctaaag
     9181 aagccttggc tcaagctaac aaacgcaagg aaccagagga aatctttgaa gaagcggcta
     9241 agttagctga acaagcacag caagttcaag cagtggttga agtcgtccct gagaaaaaag
     9301 aacctgcagt ggatacacgt cgtaaaaaac aagctcgacc agacaaaaat cgtgacgatt
     9361 atgatcatga agaagatggt cctagaaaac aacaaaagaa tcgaagtagt caaaatcaag
     9421 tgagaaatca aaagaatagt aactggaata acaacaaaaa gaacaaaaaa ggcaataaca
     9481 agaacaaccg taatcagact ccaaaacctg ttacggagcg taaattccat gaattgccaa
     9541 cagaatttga atatacagat ggtatgaccg ttgcggaaat cgcaaaacgt atcaaacgtg
     9601 aaccagctga aatcgttaag aaacttttca tgatgggtgt catggccaca caaaaccaat
     9661 ccttggatgg ggaaacaatt gaactcctca tggtggatta cggtatcgaa gccaaacaaa
     9721 aggttgaagt ggataatgct gacatcgaac gtttctttgt cgaagatggt tatctcaatg
     9781 aagatgaatt ggttgagcgt ccaccagttg ttactatcat gggacacgtt gaccacggta
     9841 aaacaaccct tttggatact cttcgtaact cacgtgttgc gacaggtgaa gcaggtggta
     9901 ttactcagca tatcggtgcc taccaaatcg tggaaaatgg taagaagatt accttccttg
     9961 atacaccagg acacgcggcc tttacatcaa tgcgtgcgcg tggtgcttct gttaccgata
    10021 ttacgatctt ggtcgtagcg gcagatgacg gggttatgcc tcagactatt gaagccatca
    10081 accactcaaa agcagctaac gttccaatca tcgtagccat taacaagatt gataaaccag
    10141 gtgctaaccc agaacgcgtt atcggtgaat tggcagagca tggtgtgatg tcaaccgctt
    10201 ggggtggaga ttctgaattt gttgaaatct cagctaaatt caaccaaaat atcgaagaat
    10261 tgttggaaac agtccttctt gtagctgaaa tccaagaact caaggcagac ccaacagtgc
    10321 gtgcaatcgg tacggttatc gaagcgcgct tggataaagg aaaaggtgcg gtcgcaaccc
    10381 ttcttgtaca acaaggtacc ttgaatgttc aagacccaat cgttgtcgga aataccttcg
    10441 gtcgtgtccg tgctatgacc aacgaccttg gtcgtcgtgt taaagttgct ggaccatcaa
    10501 caccagtctc tatcacaggt ttgaacgaag caccgatggc gggtgaccac tttgccgttt
    10561 acgaggatga aaaatctgcg cgtgcagcag gtgaagagcg tgccaaacgt gccctcatga
    10621 aacaacgtca agctacccaa cgtgttagcc ttgaaaacct ctttgatacc cttaaagctg
    10681 gggaactcaa atctgttaat gttatcatca aggctgatgt acaaggttct gttgaagccc
    10741 tttctgcctc acttcaaaag attgacgtgg aaggtgtcaa agtgactatc gtccactcag
    10801 cggtcggtgc tatcaacgaa tcagacgtga cccttgccga agcttcaaat gcctttatcg
    10861 ttggtttcaa cgtacgccct acaccacaag ctcgtcaaca agcagaagct gacgatgtgg
    10921 aaatccgtct tcacagcatt atctacaagg ttatcgaaga gatggaagaa gctatgaaag
    10981 ggatgcttga tccagaattt gaagaaaaag ttattggtga agcggttatc cgtgaaacct
    11041 tcaaggtgtc taaagtggga actatcggtg gatttatggt tatcaacggt aaggttgccc
    11101 gtgactctaa agtccgtgtt atccgtgatg gtgtcgttat ctatgatggt gaactcgcaa
    11161 gcttgaaaca ctataaagac gacgttaagg aagttacaaa cggccgtgaa ggtggattga
    11221 tgatcgacgg ctacaatgat attaagatgg atgatgtgat tgaggcgtat gtcatggaag
    11281 aaatcaagag ataagatttt ttgctccttt cttaggtggt gagggacgca agcaaaccga
    11341 tggtttcatt gcttattttt gagcctaggg tctcaaaaat cccctgtgat gggactgata
    11401 aatcagttcc atcactttca ccacggcgaa agaagcagat gacttcaaat tgaacttcgt
    11461 ttcaatttaa actgaaaatc aagaagttta aaatagctag gtctgctggc ctagcttttg
    11521 gttcaaagta gagaaaggaa tatcatggca aatcatttcc gtacagatcg tgtgggcatg
    11581 gaaatcaagc gtgaagtcaa tgagattttg caaaagaaag tccgtgatcc acgtgtccaa
    11641 ggtgtgacca tcacagatgt tcagatgctg ggtgacttgt ctgttgccaa ggtttattac
    11701 accattttga gtaaccttgc ttcggataac caaaaagccc aaatcgggct tgaaaaagca
    11761 actggtacca tcaaacgtga acttggtcgc aatttgaaat tgtacaaaat cccagatttg
    11821 accttcgtca aagacgagtc catcgagtat ggaaacaaga ttgacgagat gctacgcaat
    11881 ctggataaga actaaagaag aggggttgcc cctctttttt ggtggaggaa aataggttga
    11941 atttgaaatg gaaaaatatt cttttaaa
//
LOCUS       DBERCA010000054        11591 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.054, whole genome shotgun sequence.
ACCESSION   DBERCA010000054 DBERCA010000000
VERSION     DBERCA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.054"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            432..1142
                     /gene="deoD"
                     /locus_tag="ACL719_001638"
     CDS             432..1142
                     /gene="deoD"
                     /locus_tag="ACL719_001638"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS0699572.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1165..1689)
                     /locus_tag="ACL719_001639"
     CDS             complement(1165..1689)
                     /locus_tag="ACL719_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGS0699573.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1742..1978)
                     /gene="rpsT"
                     /locus_tag="ACL719_001640"
     CDS             complement(1742..1978)
                     /gene="rpsT"
                     /locus_tag="ACL719_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGS0699574.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2046..2966)
                     /gene="coaA"
                     /locus_tag="ACL719_001641"
     CDS             complement(2046..2966)
                     /gene="coaA"
                     /locus_tag="ACL719_001641"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGS0699575.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3156..3559
                     /locus_tag="ACL719_001642"
                     /pseudo
     CDS             3156..3559
                     /locus_tag="ACL719_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3766..4356
                     /locus_tag="ACL719_001643"
     CDS             3766..4356
                     /locus_tag="ACL719_001643"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0699576.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4353..5630
                     /locus_tag="ACL719_001644"
     CDS             4353..5630
                     /locus_tag="ACL719_001644"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGS0699577.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5648..6310
                     /gene="deoC"
                     /locus_tag="ACL719_001645"
     CDS             5648..6310
                     /gene="deoC"
                     /locus_tag="ACL719_001645"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGS0699578.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6297..6686
                     /locus_tag="ACL719_001646"
     CDS             6297..6686
                     /locus_tag="ACL719_001646"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS0699579.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6777..7829
                     /locus_tag="ACL719_001647"
     CDS             6777..7829
                     /locus_tag="ACL719_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGS0699580.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7973..9508
                     /locus_tag="ACL719_001648"
     CDS             7973..9508
                     /locus_tag="ACL719_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699581.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9501..10559
                     /locus_tag="ACL719_001649"
     CDS             9501..10559
                     /locus_tag="ACL719_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699582.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10562..11518
                     /locus_tag="ACL719_001650"
     CDS             10562..11518
                     /locus_tag="ACL719_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699583.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3304 a         2075 c         2653 g         3559 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa aggaattgac cccaccctaa
      181 aagtagtggg aaaaagatag ttggtctagc gagcatcgct cactgcgccc aactcctatt
      241 ttcccttcgc tttttgatgg gtttggtatc tttctcaata taaaatataa aataaagaaa
      301 ggtagagcgt gtgttttgat ttgaacacga gcggaaaact cggaaaatag ataatctgac
      361 tgaaaaatca ggatttctcg tcaggttcct aattttcagt cgttttcttc tcgctctttg
      421 tatcataaat tatgtctatc catattgctg ctcagcaggg tgaaattgct gataaaattc
      481 ttcttcctgg ggatcctctt cgtgctaagt ttattgcgga gaatttcctt ggtgatgctg
      541 tttgttttaa cgaagtgcgt aacatgtttg gttacactgg tacttacaag ggtcaccgtg
      601 tatctgtcat gggaactggg atgggaatgc catctatttc gatttatgcg cgtgagttaa
      661 tcgtagacta cggtgtgaag aaattgattc gtgtgggaac tgcaggttct ttgaatgaag
      721 aggttcatgt tcgtgaatta gttttggcgc aggcggctgc aaccaactca aacatcgttc
      781 gtaatgactg gccacagtac gattttccac aaattgctag ctttgatttg cttgataaag
      841 cctaccatat cgccaaagaa cttggtatga ctactcacgt tgggaacgtt ttgtcatctg
      901 atgtctttta ctcaaattac tttgaaaaga atatcgagct tggtaaatgg ggagtcaagg
      961 ctgtggaaat ggaagcagca gctctttact atcttgctgc ccaataccat gttgatgcgc
     1021 tagctatcat gaccatctct gatagcttgg tcaatccaga cgaagacaca actgcagaag
     1081 aacgtcaaaa taccttcact gatatgatga aggttggttt ggaaaccttg attgcagaat
     1141 aattatagcc aaaaaggaag ccctttatga attgtaggac ttccttttct tatccagaaa
     1201 ttgatctagc tctctctgat ttcgaagaat agtgacttta tgtgaatatt cttggcaaag
     1261 tttttggtaa ttttcttttt gagttttgct acgcccatcc caaagaatcc atctgataaa
     1321 ctcccagtca aagcgttcag ggcaatctgc cgccatactt tctctgactt ttccacggta
     1381 tttaagataa cgcttaaagg ctctaaagag acaggtcaat ggcaaaaaat tgagaaagat
     1441 gatttggtca gcttcttgca ttcgttcttg gtagtagcac caagaataat taccatcgat
     1501 gacccaagct ttatgcttgg tgagaaagtt ttttatctcg gttaacatcc attcgcagtc
     1561 actgtcttgc caaccaggtt gaaattggag tgtgtccatg tgcagttttg gaatggagta
     1621 gtagttagat aacttttctg ctagagttga cttaccagaa ccagaatatc cgataattgt
     1681 gattttcatt ttctaccttt tcctatttgg agacaaaaaa acagcctcta tggactgttt
     1741 cttatttagc aagtttagtt gaaagacgag ctttatcgcg gcttgctttg tttttgtgaa
     1801 tcaaaccttt agtttctgct ttatcgatag ctgagctagc agcacggaaa agttcttcag
     1861 atgggtttgc ttcgaaagct tttatagcag tacgcatagc tgatttttga gctgagttct
     1921 tttcgttttg tttaacgttc aattcagcgc gtttgatagc tgatttaatg tttgccaatg
     1981 ttcttacctc catatttact aactatacca ttatatatga aaacttacgt tttgacaagg
     2041 gaaaattact tttttaagta aatttcatcg atttcatggt tcttgctttt atgaagaatc
     2101 acttctgcac gatttctggt tggttcaata taattttgca gatttgtgag attgatactg
     2161 atccagacct gatgggcaaa ggactccact tccccaatcg gcatctgagt aaaacgataa
     2221 tagtagctat cagggtcgtt ttgggctagg ctcagcatct tcaagaaacg gtccagatac
     2281 caactttcaa tatcatccac tccagcatct acatagatgg aaaagtcaaa gaagtcagtg
     2341 atatagagac gatcgttttg tggattttga aagacattaa ttccctcaac aattacaaaa
     2401 tcagcagctt tgacactttg tttcttttcg ggtacgatgt cgtaaacttc atgagaatag
     2461 acaggaatat ctacatcttg tccatttttg atgcggtcca agaagttgag aagagcttcc
     2521 atatcatagc tttcaggaaa tcctttacga tttaaaatcc cctgctcaat caaggtttga
     2581 ttgggataga gaaaaccatc agttgtaacc aactcaaccg tagcatctgt aaacgtacgg
     2641 gacagtagga tttgaagtag gcgactggtt gtggattttc caacggcaac actcccagaa
     2701 accccaataa taaaaggttg agatttactt tcacgttgga ggaaaattcc ttttgaaaag
     2761 gctaaatctt ccttagttcg cttgtaaatc tgaatcagat gagccaaggg gagatagata
     2821 tctgtaacgt cttggagact gatttggtca ttaaaactct tgatagattc caattcttct
     2881 tctgtcaaag gaggtgttgt ctttcgatgt aaagattgcc aagtctggcg gctgattttt
     2941 tcaaaatgta aaaattcgtt ggtcatttgt tttcccctag atattttgtc tatcatacca
     3001 taaaaagcta aaaaaataaa gcggtttctt gaatgtggta agtcaatggt atatagtata
     3061 agtgatcaat ttattgatga aataggagat agattctact gatttataag cttataaagg
     3121 gggcttttta gttcacaact tataggaggt gtactatgaa aatcttaaaa cgttacatat
     3181 tggaactctg ttttatttta agttttgctt taccttttat aaaaggaacc aatgcagata
     3241 atggtagatg ctttgtggaa acctattacg gttttacttt tttgatggaa catgctattg
     3301 taacagctgt ctttatctgt tcgttcttaa ttgctttctt actaaaaaac gatggacgaa
     3361 atggattgct gcgggtagtt attgcttttt agtcctacgg attgcgacag agggttattt
     3421 cttccacatg tcactagaag atttgatacg actttggaca agtttggaaa tcctgacaca
     3481 aacgtatcag ttgggctttt atctaaacat tttgttagga attttgttga taataaagta
     3541 ttttaaggtt aagcaataga aataataatc tgcctgatta tagatgataa actttttgtt
     3601 tttatctgga ctgtgatatt tatagttcta aatggtttat tatttataag tgttgaaaga
     3661 gattgaagaa aatggcttcg tgacttgctt tcttcggtct ttttcgtttt attatcaatt
     3721 tgtaaacgat ttacaatttg agtcaaatta tggtaaaata gtgtcatgag taaaatgtac
     3781 tatgcagaaa atcctgacgc tgctcacgac attcatgagt tgagagtgga cttgttggga
     3841 gaaaaaatga cttttttgac agatgcgggt gtttttagca agaaaatggt tgactttgga
     3901 agtcaactct tgctcaagtg tctggatgtc aaccaaggag agacagtcct agatgtaggc
     3961 tgtggttatg gtccattagg tttgtccttg gtcaaggttt acggagttca ggcgaccatg
     4021 gttgatatta acactcgtgc cctggattta gctcagagaa atgctgaaaa aaataatgca
     4081 aaagcgacga tatttcaatc taatatttat gcaagagttc aagggcgatt tgaccatgtc
     4141 atttccaatc ctcctatccg tgcgggcaag caagtcgttc atgaaatcat cgagaaaagt
     4201 aaagatttct tggaaattgg tggagatttg accatcgtca tccagaaaaa acaaggagct
     4261 ccaagtgcca aatctaagat ggaagatgtg tttggcaatt gtgaaatcct taaaaaagac
     4321 aagggatact atatccttag aagtgtgaaa gaatgagagc agttgattta atccaaaaaa
     4381 aacgagacgg tcaagaactg acttcaagtg aaattgaatg gctgatagaa ggctatgtat
     4441 caggaactgt tcctgattat cagatgtctg cctttgctat ggctgtttat tttaaaggaa
     4501 tgacgactcg agaaatctct gatttaacga tgaatatggt taagactggg caagagtttg
     4561 acctgtcagc cattgatggt gtgaaagttg acaagcattc tactggtggt gtcggcgata
     4621 aggtaacctt gattttagct cctcttgttg ctagcttcgg tgtgcctgta gcaaaaatga
     4681 gtggtcgtgg tcttggccat acaggcggaa caattgataa attggagtcc attaggggct
     4741 atcaagtaga acgtagtcaa gaggatttta ttcgtcaggt tcaggatatt ggagtatctg
     4801 tcattgggca atcagaccag ctggtcaaag cagataagct tctctatgcc cttcgtgatg
     4861 tgaccgcaac tgtcgacacg attcctttga ttgcgagttc ggtgatgagc aagaaaattg
     4921 cggcaggggc ggatgctatt ttgctagacg tgactgtcgg tgagggtgcc ttcatgaaga
     4981 cggttgatga ggcgcgtgag ttggctcaaa ctatggtaga tcttggtaag gttgttggac
     5041 ggaagacagt agcagtgatt acggatatga gccagccttt gggacgagcg attggaaatc
     5101 gtctggaaat ccttgaagca ttggagattt tgcaaggtca aggacgtcag gatattaccc
     5161 actttatttg tgaattggct caaattatgc ttggtttggc aaatgttaac aaaactgttg
     5221 aagaagttcg ccaacatctt gaaaatggtc aagcactggc taagtttgag gaaatggtgc
     5281 aagcccaagg tggagacttg gaagacctct atcgtcctgt aaatgtagct catgtggtgg
     5341 aaatccctgc tcaggaaacg ggtgtcattt cagctcttcc agctatggac tttggtcttt
     5401 atgccatgag gctgggtgct ggtcgtgcag ttaagtctga tgtcttggac tatgaaacag
     5461 gaattgtttt tgaaaagaag attggagact ccgttcaaaa gggtgaaatt gttgcaaaag
     5521 tatacacaaa tgaaaaaatt ccacctcaac tagttacaga tttccaaaaa tgtgttaaaa
     5581 taagtgatga agtgaaaaaa atccgtgaaa ttgtagaaat catctcctaa atggagggaa
     5641 aaccgaaatg aaattaaata aatatataga tcatacgctt ttaaaacaag atgcaaagaa
     5701 aaaacaaatt gatagtttgt tgtctgaggc tagagagtat ggctttgcca gtgtttgcgt
     5761 taatccgacc tgggttgaac atgctaaaaa aggacttgaa ggcacagatg ttaaggtttg
     5821 cacagtagta ggtttcccct tgggagcaac aacttcaact gtgaaagctt ttgaaacaaa
     5881 agaagctatc caaaatgggg ctgatgagat tgacatggta atcaatgttg gggctctcaa
     5941 gtcaggtgat ttggccttgg ttgagtcgga tattcgtgca gtagtggaag caagtgggga
     6001 caagttagtg aaagttatta ttgaagcttg ccttctgaca gaccaagaaa aagttttggc
     6061 ttgccaatta gcccaaaaag caggagctga ctttgtcaaa acatctactg gcttttcaac
     6121 tggtggtgct acgatagcag atgttagatt aatgcgtgaa acagttggac ctgatatggg
     6181 tgtcaaggcc gccggtggag ctcgttctta tgcagatgct cttacctttg tcgaagcagg
     6241 tgcgacccgt atcggaacgt cagctggggt agctatttta aaaggagaat tggcagatgg
     6301 cgactactga gttgattgaa ctggcaattg aaaccagcaa acatgcctat gtcccctatt
     6361 ctcactttcc tatcggagca gttttagtag ccaaagacgg aagtgtttac acgggagtga
     6421 atatcgaaaa tgctagctat cctttgacca attgcggtga gagaacagct atttttaagg
     6481 ctatatctga aggccaaaga gaattttcag aattgattgt ctatggtcaa actgaaaaac
     6541 cgatttcacc atgtggtgct tgtcgccaag taatggtcga attttttgaa caagatttaa
     6601 aagtgaccct agtcgcaaaa gataaatcga cggtcgagat gacggtcggg gagttacttc
     6661 catactcttt tacagactta aattagtctg agtcgctctt tgagtggcaa gggtctttgt
     6721 gaccaatcaa tccatacttg caacatcgtt gcacatctta tttaggaggt tcagtaatga
     6781 acaagaaaca atggctaggt cttggcctag ttgcagtggc agcagttgga cttgctgcat
     6841 gtggtaaccg ctcttctcgt aacgcagctt catcttctga tgtgaagaca aaagcagcaa
     6901 tcgtcactga tactggtggt gttgatgaca aatcattcaa ccaatcagct tgggaaggtt
     6961 tgcaagactg gggtaaagaa cacaatcttt caaaagataa cggtttcact tacttccaat
     7021 caacaagtga agctgactac gctaacaact tgcaacaagc ggctggaagt tacaacctaa
     7081 tctttggtgt tggttttgcc cttcacaatg cagttgaaga agcagctaaa gaacacactg
     7141 acttgaacta tgtcttgatt gatgatgtga ttaaagacca aaaaaatgtt gcgagcgtaa
     7201 ctttcgctga taatgagtca ggttaccttg caggtgtggc tgcagcaaaa acaactaaga
     7261 caaaacaagt tggttttgta ggtggtatcg aatctgaagt tatctctcgt tttgaggcag
     7321 gattcaaggc tggtgttgcg tcagtagacc catctatcaa agtacaagtt gactacgctg
     7381 gttcatttgg tgatgcggct aaaggtaaaa caattgcagc cgcacaatac gcagccggtg
     7441 cagatattgt ttaccaagta gctggtggta caggtgcagg tgtctttgca gaggcaaaat
     7501 ctctcaacga aagccgtcct gaaaatgaaa aagtttgggt tatcggtgtt gaccgtgacc
     7561 aagaagcaga aggtaaatac acttctaaag atggcaaaga atcaaacttt gttcttgtat
     7621 ctactttgaa acaagttggt acaactgtaa aagatatttc taacaaggca gaaaaaggag
     7681 aattccctgg cggtcaagtg atcgtttact cattgaagga taaaggggtt gacttggcag
     7741 taacaaacct ttcagaagaa ggtaaaaaag ctgtcgaaga tgcaaaagct aaaatccttg
     7801 atggaagcgt aaaagttcct gaaaaataat ggatggaagt catttcttag gaagcggcca
     7861 agggccgctt ttttaagagt tgagacaaag tcattttgtc tcagtaaagc ttgctactag
     7921 acaaactagc aagttttatt gaaataaaat aagactctga aaggaagagc acatggcaca
     7981 cgaaaatgtc attgagatgc gtgatattac caaggtgttt ggtggatttg ttgccaacga
     8041 caaaatcaac ttgcacctac gaaaaggtga aattcatgca cttttaggag aaaatggggc
     8101 tggtaagtcc acgctaatga acatgttagc aggccttctt gaaccaacca gtggtgaaat
     8161 cgcggtcaac ggtcaagttg tcaatctcga ctccccatct aaagcagcta gcttgggaat
     8221 cgggatggtt caccagcact ttatgttggt tgaagccttc acagtggctg aaaacatcat
     8281 tttaggtagt gaattgacta aaaatggtgt gctagatatc gctggagcta gcaaagaaat
     8341 caaggctctt tctgaacgtt atggcttagc tgttgaccct tctgccaagg tagcagatat
     8401 ctcagttgga gcccaacaac gtgtagaaat tttaaaaaca ctttatcggg gggctgatat
     8461 ccttatcttt gacgaaccaa cggctgtttt gactccatca gaaattgatg agttgatggc
     8521 tattatgaaa aatcttgtca aagaaggaaa atcaattatc ttgattaccc acaaattgga
     8581 tgaaattcgc gcagtttctg accgtgttac agttatccgt cgtgggaaat caattgaaac
     8641 cgttgaaatt gcaggggcta ccaatgctga tttggcggaa atgatggtag gacgttctgt
     8701 ttcctttaaa acagagaagc aagcctctaa accaaaagaa gtggttttgt ctatcaaaga
     8761 tttggtggtc aatgaaaacc gtggtgttcc agctgttaaa aatctgtcct tggatgttcg
     8821 tgctggagag attgttggta ttgcggggat tgatggaaat ggtcagtctg aactgattca
     8881 agccattaca ggtcttcgta aggttgaatc tggtagcatt gagctaaaag gagattcaat
     8941 tgtaggcttg cacccacgtc agattacaga actaagtgtt gggcacgttc cagaagaccg
     9001 tcaccgtgat ggcttgattt tggaaatgat gatatctgaa aatattgccc ttcaaaccta
     9061 ctataaagaa ccacatagta aaaatggaat tttgaattat tcaaatatta cttcttatgc
     9121 taaaaagctg atggaagagt ttgatgttcg cgctgccagt gaattagttc ctgcagctgc
     9181 actctcagga ggaaatcaac aaaaagcaat tattgctcgt gaaattgatc gagatcctga
     9241 tctccttatc gttagccagc caactcgtgg gttggatgtc ggtgccattg agtatatcca
     9301 caaacgcttg attgaagagc gtgataatgg caaggctgtc cttgttgtca gctttgaatt
     9361 ggatgagatt ttaaacgtct cagaccgtat tgccgttatc cacgatggta agattcaagg
     9421 tattgtatca ccagaaacaa ccaataaaca aggacttggt gtcttgatgg ctggtggaaa
     9481 cttgggaaag gagaagagtg atgtctaaaa aattacaaca aatttcggtt cccttgattt
     9541 ctgtattcct aggaatttta ctcggagcca ttgtcatgtg gatcttcggt tatgatgcta
     9601 tttggggcta cgaagaattg ttctatacag cctttggcag tctgcgtggg attggagaaa
     9661 tcttccgtgc tatgggtcct ctggtcttga ttggtcttgg ttttgcggtt gccagtcgag
     9721 ctggtttctt taacgtcgga cttcctggtc aggctttggc aggttggatt ctcagtggtt
     9781 ggtttgccct gtcgcatcca gatatgcccc gtcccttgat gattctagca accatcgtga
     9841 ttgccttgat tgctggtggg attgtcggag cgattccagg tattcttagg gcctatctag
     9901 ggacgtcaga ggttattgta accatcatga tgaactacat tgtcttgtat gtagggaatg
     9961 cctttatcca tgctttccct aaagacttca tgcaaagtac agattcgacc attcgtgttg
    10021 gggctaatgc aacctatcag acaccttggt tagctgagtt gactggtaac tcacggatga
    10081 atattggtat tttctttgcc atcattgccg ttgcagttat ttggttcatg ctcaagaaaa
    10141 caactcttgg ttttgaaatc cgtgcagttg gtcttaatcc acatgcttca gaatatgctg
    10201 gtatttctgc caagcggact attatcctat ctatgattat ttcaggtgcc ttggcaggtc
    10261 ttggtggagc tgttgaaggt ttgggaacct tccagaacgt ctatgttcaa ggttcgtcat
    10321 tagctatcgg atttaacgga atggcggtta gtttgcttgc ggccaactca ccaattggta
    10381 tactctttgc agccttccta tttggcgttc tccaagttgg ggctcctggt atgaatgcgg
    10441 cgcaggtacc atctgagctt gtcagcattg taacagcgtc tattatcttc tttgtcagtg
    10501 ttcattacct tatcgaacgc tttgtcaaac cgaaaaaaca agttaaagga ggtaagtaaa
    10561 gatgtctatt ataaccttgc tccccctctt ggtgtcttct atgctgattt actcagcacc
    10621 tctcatcttt acaagtatcg gtggtgtttt ctctgaacgt ggtggtgtgg taaacgttgg
    10681 ccttgaagga attatggtta tgggtgcctt ttctggagtt gtctttaacc ttgaatttgc
    10741 agaacaattt ggagcagcaa ctccatggct atccttgctt gtagcaggat tggttggtag
    10801 tgttttctct atcatccacg cagcagcgac ggttcatttc cgtgcagacc atgttgtcag
    10861 cggtacggta ttgaacttga tggcgcctgc cttggctgtt ttcttagtta aagttcttta
    10921 taacaaagga caaaccgaca acctaagtca aacttttgga cgctttgatt tcccagtctt
    10981 ggcaaatatc ccagtgattg gtgatatctt cttcaagtca actagtctac ttggttatct
    11041 ggcgattgcc ttctcattcc ttgcttggtt tattctcttt aagactcaat ttggtcttcg
    11101 tctccgctct gtcggtgaac atcctcaagc agcggatacc ttgggaatca acgtctacaa
    11161 gatgagatat ttaggggtta ttatttcagg ttttctaggt ggaattggcg gagcgattta
    11221 tgcgcaatca atctcagtta acttctcagt gacaactatt gttggacctg gatttatcgc
    11281 ccttgctgcg atgatctttg ggaaatggaa tccaatcggt gctatgcttt ctagtctctt
    11341 ctttggactt tcacaaagtt tggctgttat cggttctcaa ttgccgttcc tacaaggagt
    11401 gccagcggtt tatcttcaaa ttgcacctta tgttttgaca attcttgtct tagcagcctt
    11461 ctttggaaaa gcagtcgcac caaaagcaga tggtatcaac tacatcaaat caaaataagc
    11521 atacaaaaaa acgtcagtcc aatgactgaa ctgcccccca aaagttagac agaaaaaatc
    11581 taacttttgg g
//
LOCUS       DBERCA010000055        10961 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.055, whole genome shotgun sequence.
ACCESSION   DBERCA010000055 DBERCA010000000
VERSION     DBERCA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.055"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            12..734
                     /locus_tag="ACL719_001651"
     CDS             12..734
                     /locus_tag="ACL719_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS0699584.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            731..1579
                     /locus_tag="ACL719_001652"
     CDS             731..1579
                     /locus_tag="ACL719_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGS0699585.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1611..2540
                     /gene="psaA"
                     /locus_tag="ACL719_001653"
     CDS             1611..2540
                     /gene="psaA"
                     /locus_tag="ACL719_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGS0699586.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEANLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2663..3154
                     /gene="tpx"
                     /locus_tag="ACL719_001654"
     CDS             2663..3154
                     /gene="tpx"
                     /locus_tag="ACL719_001654"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGS0699587.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3250..5958)
                     /locus_tag="ACL719_001655"
     CDS             complement(3250..5958)
                     /locus_tag="ACL719_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS0699588.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALTTHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLRLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVVAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5960..6661)
                     /locus_tag="ACL719_001656"
     CDS             complement(5960..6661)
                     /locus_tag="ACL719_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699589.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6911..9322)
                     /locus_tag="ACL719_001657"
     CDS             complement(6911..9322)
                     /locus_tag="ACL719_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS0699590.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGRI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9375..10067)
                     /locus_tag="ACL719_001658"
     CDS             complement(9375..10067)
                     /locus_tag="ACL719_001658"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGS0699591.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10602..10961)
                     /locus_tag="ACL719_001659"
                     /pseudo
     CDS             complement(10602..10961)
                     /locus_tag="ACL719_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3204 a         2519 c         2047 g         3191 t
ORIGIN      
        1 aatataagtt tatgatacgt atcgaaaacc tcagtgtctc ctacaaagaa acgttggcac
       61 ttaaggatat ttcactagtg ctccatggac caacaattac cggcatcatt ggtccaaacg
      121 gcgctgggaa atcaacacta ttaaaaggta tgttgggaat tatcccacat caaggtcagg
      181 catttctcga tgacaaggaa gttaaaaaat ccttacaccg aattgcctat gtcgaacaaa
      241 aaatcaatat cgactacaac tttcccatca aggtcaagga atgcgtctcg ttaggactat
      301 ttccctctat tcctctcttt cgaagtttaa aggctaaaca ttggaagaaa gtgcaagagg
      361 cccttgaaat cgtcggccta gctgactacg ctgaacgtca aattagtcaa ctgtctggag
      421 gtcaattcca gcgggtcttg attgccagat gtttggtgca ggaagccgac tatatcctct
      481 tggatgaacc ctttgctggg attgactctg tcagtgagga aatcatcatg aatacgctga
      541 gagatttgaa aaaagctggg aagacggttc tcatcgttca ccacgacctc agcaagattc
      601 cccactactt cgatcaagtc ttacttgtca atcgagaagt gattgccttt ggtccaacaa
      661 aagaaacttt taccgaaacc aatctaaaag aagcttacgg taatcaactc tttttcaatg
      721 gaggtgacct atgattgcag aatttatcga tggattgcaa aaattccatt tcctacaaaa
      781 tgccttgata acagctattg tcgtcgggat tgtagctgga gctgtgggat gtttcattat
      841 tctacgcggg atgtcactca tgggagatgc catttcacat gctgtcttac caggtgtagc
      901 cctatccttc atcttgggcc ttgacttctt tatcggagcc attgtctttg gattgctagc
      961 tgccatcatc attacctaca tcaaaggaaa ctcgattatc aaaagcgata ccgccatcgg
     1021 cattaccttt tcttctttct tagccctcgg tatcatcttg attggtgtcg ctaaaagttc
     1081 aactgacctt ttccatatcc tttttggtaa tatcctggcc gtccaagata cggatatgtt
     1141 tattactatg ggtgtggggg cagctatcct cttgttaatc tggattttct tcaagcaact
     1201 cttgataact tcctttgatg aactcttggc taaagccatg ggaatgcctg tcaatttcta
     1261 tcactacctt ctcatggtac tcctgactct cgtgtctgtg acagccatgc aaagtgtcgg
     1321 aactatcctg attgtagcca tgctgattac cccagctgca actgcttatc tgtatgctaa
     1381 tagtctgaaa agtatgattt tcctttcctc aaccttcgga gctactgctt cagttttggg
     1441 actctttatt ggctatagtt ttaatgttgc ggcaggttct agtatcgtgc ttacagctgc
     1501 tagtttcttt ctcattagct tctttatcgc tcccaaacaa cgatatttga aactgaaaaa
     1561 taaacatttg ttaaaataag gggcaaagcc ctaataaatt ggaggatcta atgaaaaaat
     1621 taggtacatt actcgttctc tttctttctg caatcattct tgtagcatgt gctagcggaa
     1681 aaaaagatac aacttctggt caaaaactaa aagttgttgc tacaaactca atcatcgctg
     1741 atattactaa aaatattgct ggtgacaaaa ttgaccttca tagtatcgtt ccgattgggc
     1801 aagacccaca cgaatacgaa ccacttcctg aagacgttaa gaaaacttct gaggctaatt
     1861 tgattttcta taacggtatc aaccttgaaa caggtggcaa tgcttggttt acaaaattgg
     1921 tagaaaatgc caagaaaact gaaaacaaag actacttcgc agtcagcgac ggcgttgatg
     1981 ttatctacct tgaaggtcaa aatgaaaaag gaaaagaaga cccacacgct tggcttaacc
     2041 ttgaaaacgg tattattttt gctaaaaata tcgccaaaca attgagcgcc aaagacccta
     2101 acaataaaga attctatgaa aaaaatctca aagaatatac tgataagtta gacaaacttg
     2161 ataaagaaag taaggataaa tttaataaga tccctgctga aaagaaactc attgtaacca
     2221 gcgaaggagc attcaaatac ttctctaaag cctatggtgt cccaagtgct tacatctggg
     2281 aaatcaatac tgaagaagaa ggaactcctg aacaaatcaa gaccttggtt gaaaaacttc
     2341 gccaaacaaa agttccatca ctctttgtag aatcaagtgt ggatgaccgt ccaatgaaaa
     2401 ctgtttctca agacacaaac atcccaatct acgctcaaat ctttactgac tctatcgcag
     2461 aacaaggtaa agaaggcgac agctactaca gcatgatgaa atacaacctt gacaagattg
     2521 ctgaaggatt ggcaaaataa acctatgaaa aacgtcattc tcacatgagc tggcgttttt
     2581 tctatgccca tatttccggt caaatcattg gaaaattccg actgtttcag ctaaaatgga
     2641 agaaaaaaga ttggagtatc ctatggtaac ttttctcgga aatcctgtga gctttacagg
     2701 taaacaacta caagtcggcg acaaggcgct tgatttttct cttactacaa cagatctttc
     2761 taaaaaatct ctggctgatt ttgatggcaa gaaaaaagtc ttgagtgtcg ttccttctat
     2821 cgatacaggc atctgctcaa ctcaaacacg tcgttttaat gaagaattgg ctggactgga
     2881 caacacggtc gtattgactg tttcaatgga cctacctttt gctcaaaaac gttggtgcgg
     2941 tgctgaaggc cttgacaatg ccattatgct ttcagactac tttgaccatt ctttcgggcg
     3001 cgattatgcc ctcttgatca acgaatggca cctattagca cgcgcagtct ttgtcctcga
     3061 tactgacaat acgattcgct acgttgaata cgtggataat atcaattctg agccaaactt
     3121 cgaagccgca attgcagctg ctaaagccct atagaaaaaa tcctctgtca caaagagttc
     3181 cctgctcttt gaaacggagg atttttgaat attagtaaat agaagttcaa tcagctaaaa
     3241 ataactacct tactctacag atttcagggc ttctaacata tcaacctttc tcagataata
     3301 attgacgaag aaaccaagca aggtcaaaat gatgcttact gctgccactg ggattacata
     3361 gacttcccag cctacctgcg gataaaagag aatagtcgca ggcgaaatca tttgaatcaa
     3421 aaattggtgt aaatagaaac cagctatcag accaagtacg attcccacaa gggacagcac
     3481 aatcgtctca cggtaaatgt agagggtgac ttcattatta tgaaaaccaa gaaccttgat
     3541 agtggagagt tcacggattc tctcagctac gttgatattg gtcagattgt aaaggatgac
     3601 aatagctaat agaaccgata cgatgaccaa gatggtcatg gtctgattga gtgagctagc
     3661 gatagagtcg aagagtcgaa tggctgaagc attttggaca acgctggaca ccgcagattg
     3721 attcataagc aagcccgcct gactttcgat actagttgca ctggtatccc ttaatgagac
     3781 cagataagtg ttggcttggg gtagctgtcc gtaaagttgc tcatagctag cctgactcat
     3841 ataaataaag tgaccaacgt agttctcagt aatagcaacg acctttagtt ccttaccttc
     3901 aatttctaaa gtctgcccaa ccttgacacc tgccagctgg gcgagtttag ctgtaataac
     3961 gatgccatct tttaatgtca gctcctgctg atgatgttga agatggataa agggagtcaa
     4021 atcttccttc tctatcatca taagagtaat gttttgaaga ccagctttgc ctttgaaatc
     4081 cttgtatagc gctttagaat agattttctg gtaggctagt atctcctgcc ctttcaacac
     4141 ttctgctagc tctaccttgt cctgattggt cgcactagga ttttcagaga caagcatctg
     4201 atactgttgg atttgttgaa actgtttaga cggaactcct gctacagaag attggattcc
     4261 caaacctgca aagagcagag ctacagaacc tgccacacca aagattgtca tcaacattct
     4321 ctgcttataa cgaaagatgt tgcgggctgt taccttatga gtaaaactga gacgacgcca
     4381 gataaaaccg atacgctcca ataagatttt agctcctttg acaggaggtt taggaagtag
     4441 aagctgggct gcttcgtcat gaagttccct ccaagccacc agataggctg gtaacacact
     4501 cgccaacaag ctcaagacaa aagctagtaa gctataggtc caatagaact gaatctgagt
     4561 ttctcccacc accatgcctt ttgtaatgac acttgaaatt acactggcta gcaaataatg
     4621 accaagtata ctacctagag ccgttccgac agtcccagct actagtccat aaaggagaaa
     4681 cttggcgata atatccttac tacgataacc caaggcctta aaaatccctg catgagttcg
     4741 ctcttcgtct acaaagcgag tcatggtcgt aaaggtcacc atggctgcta cggcataaag
     4801 taccacagga aagatattgc ccactgctcg aatactggat gaagcattgc tatacataag
     4861 atagccctga ccacctggca tggtctgacg attataaacc tgataccttg gctccgccaa
     4921 atcatccaag acttgctgat gtttttctaa tttttccttt tcttgggcta gattttgttc
     4981 agcttgcttt agtttgtcct cttccttgcc caattcctgc ttggcttggg taagttgagc
     5041 actagcctgc tctctctgag cttgaggaaa caaggctagt tgactttctt gagcctgtat
     5101 acgagcttga gcagctgcta aacgacgctt gccttcctgc aaattagtct gagccttgtc
     5161 aagggtctct tgccccttgt ctagagactc ttgtccttct tttttcaaaa gtcgcagacg
     5221 taccttgcca ttatctgata aaatttgttc aagctcttcc tgatgttgct tggatttttc
     5281 ttcataagct gatgaaaagg catttaaacc tgctaaatct tgatatttca aacgagctat
     5341 attgtagact ttctgatcaa attgactagg taaaatcacc ccataggctg tcagagtccc
     5401 acttccactt cctgcgtagc ccatatctcg ctgggagagg atttcagccg aatccacaaa
     5461 accagtaatg gtataagtat ggtcttttaa agaggaatga ccctcttctt tttctttaaa
     5521 actaatctcc tgtcccacgc tgtattggcc ttgcaaatga gtggtcaaag cgatttcctt
     5581 gtctgactga ggaagtcgtc cctttcttag ctgaaaggtt gaaattcgct ctggtttgga
     5641 gtacagccga atggcatcct gcccattatc catagtcaca tctgtcaaat agccaaactc
     5701 gacctctgcg ccctccgtct gttttagttc ttcttggtct gcttgatcca agccatagtt
     5761 agacatgact gccaaatcta aggtttgagc agttgttaaa taagcattag ctgtcgcctc
     5821 catgttggga ctggttactt tgaggcctac taaggctaga gatcccaaca tcatcaggat
     5881 caagatggat aaaaaacgcc ccttggagcc tgtgaaggac tgaattaagt ccttccaata
     5941 agtttttcgc ttgatcatgc tagtactcca aactgtcaat atcctgagga tgctggttga
     6001 gcaccacatc cttgacactg gcatcgtgca tatgaatcac gcgatcagca atgggcgcca
     6061 aagctccatt atgagtcaca atgatcaccg tcgctccctt ttgacgagac atgtcttgga
     6121 gaattttcaa aacctgcttg cccgtctgat aatccaaggc tccagtcggt tcatcacaaa
     6181 ggagaatttt aggatttttg gctaccgcgc gtgcaatgga gactcgctgt tgctcccctc
     6241 cagaaagctg ggctggaaag ttatttagac gatgagccag acctacatct gtcaagacct
     6301 gatcaggatt caaggcatct gtcacaattt cagaagccag ttccacattt tccttagctg
     6361 tcagattaga aactagatta taaaactgaa aaacaaaccc cacatcattt ctacggtaat
     6421 tggtgcgctg gtgggaacta taatccgcaa tattaacacc atcaatccag atttcccctt
     6481 catcattggt atccattccc ccaagaaggt taagaactgt tgacttgcct gcacctgaag
     6541 caccaaggat aataaccagc tccccctttt caatctcaaa attcacatca cgattagcca
     6601 caatctccgt gtccccaacc tgataacgct tgtaacagtg tttcatctca atataagcca
     6661 tcagacccac cctctatcaa acaaattata ctcctcgaaa atctctcttc aaaccacgtc
     6721 agcttccatc tgcaacctca aaacactgtt ttgagcaacc tgcggctagc ttccttgttt
     6781 gtgctctttg attttcattg agtattactt cctattatca ttataacgct ttttcaacta
     6841 gttgaaaact gcttatattc ttaaatcctt attaaaaggc agtttcaaat tactgcctta
     6901 taacatcttc ttaaaaataa aattgaacaa gatcaccttc tgctgttgcc accgaaatac
     6961 aatctttccc catgcatttc gcttttattt tttcttgatt cattttaatc acactcttct
     7021 caatatctgg ataagaaact cgcaaatctc ctccgctcct tgataaaatg gtcaactgta
     7081 agagtttttt atcttcccag ctcatgctca cttcaaaatg tccacgtgcc attaagcctg
     7141 aaacagaacc tgttgaccaa gcatcaggta gggcagctag aggtaccaga taagctgcat
     7201 gagactggag taacatttct gccatgccac tagtagcacc aaaattacca tctatctgaa
     7261 aaggaggatg gctacaccaa agatttggca aggtggatgt ctttaactgc tctgccaata
     7321 atttatgggc gcgattgcca tctcccaaac gcgcccagag attgatctta ttagccttgg
     7381 accagcctgt gccgccatct ccacgatcat tgaggctagc acgcgccgct tcaatatact
     7441 cttgtccctt gtagctaaag agattgccag gatagagtcc cactagatgg gaagcgtgcc
     7501 ggtgctgggc ctccactttc tcattttgaa aatactgctc ttcctcctca taccactccc
     7561 tgattcgacc agattgagtg atttgcaaag gatttagtaa atcagacttc tccttaacct
     7621 cagtcaacaa gtcctcatcc agtcccaatt cctgagcagc ctgaataaaa tcatgaaata
     7681 actgccaaat cagagattgg tcataggtat tgccaatcga aatcggccca tgttctggag
     7741 aataagacgg agaagacacc caacgctgcg cctgctgatc cttatgtaaa aaggcattcc
     7801 aaaaacgaac cgtttccctc aacatgggat aaattttctc cctgagatag tcttggtccc
     7861 tataaaatga ataggcttca taaacggttt gcatcatcca cgcattggca gctggtgacc
     7921 aaccccaata gtaatcccaa ccaggtgccg tccaaccaaa gggagtcgct tgagtatgaa
     7981 ccaaccaacc attttcctca cctttctgag agacgattcc tgcatacttt acagccgcta
     8041 gacgaccata gacacgcaaa tcatctacat agttgatgac cggaaagacc gcctctagga
     8101 gattggtaac ataggctggc caataattca tctgcagatt gacatttaag tgatagtccg
     8161 aattccaagg aggattgtcg accgcattcc agactccctg taggttagct ggtagagcat
     8221 ctgggcagtc tctggacgaa ctaatcaata aataccgtcc atactggaag aacagctcct
     8281 ccaaagcctg cccttcttgt ggcttataat tttttaacaa atcatctgta gtggatgcgt
     8341 caacatcagc ttccaaatcc aattgaacac gctggaataa ggcttggtag tcctcgatat
     8401 gccttgattt caattgggta tagccctttt ctttagctgt gtccaccaag tctatcacct
     8461 gttgctctaa atctagtttc ttgcgataat tgctagcagg attttgggca aaatccgtct
     8521 tagcggccaa gaagagattg gcataactgg ctcctgatat ctgaacccta tctgaccaaa
     8581 ctctaatatc tccatccgtt tcccaagcta gataactagc aaaccgcaga tcattatcct
     8641 taactcttcc cttcatcaag atatgagaat cagtaatatc caacttacac tccttgtaat
     8701 cagatttttc ctgctcatac ttttcatcag aagccaaatc acaggtcaag gatagttcta
     8761 tagtaaaatc tagagtttcc gccccttcct tagtaaaacg ctgaaccaag agatcatctg
     8821 gaaaactcgc aaaagcttca cgttcaaatc gcgttccctt atagacataa gaagtcgtca
     8881 caagtgcctt actaatattc agctgtctct gatagtccgt cacctgagac aaagtcgtac
     8941 cttgctggct gaactcaatg tgaatatccc caaaagacag ataggtccca tattgactcg
     9001 tttttggccc aattaggtgc tgctcagcca gttccttagc cagattgtaa tctctcttct
     9061 ccaaagcctg ccgaatctca gctaaaaaaa catactgatc ctgaagattt ccaccctgat
     9121 aatctgaact atcaggaagt ggacctccag accagagact tttttcatta aattgaatcc
     9181 gttcagcccc tatacggcca aatacttttg ctcctaaaga accattacct atcggtaaag
     9241 cttcttcttc ccaacccatg taagtggttg aagctggttg tttgtaggcc agtacataat
     9301 cttgtttttt attccttatc atacttccat actaacataa aaagcctaga aatcaatcct
     9361 aggcttacaa ttttttattt tccaaggtag tattcagaaa caacgttcaa tttttcgtcg
     9421 aattcgaata ccaatggtgg gaagttaggg atttccacgt ccatgatctc atcatctgac
     9481 aaacctttga tgtgttttac aagggcacgg attgagttac cgtgagctcc tacgaatacg
     9541 tttttaccat ctttaagagc tggagcgatt ttatcttccc agaatggaag agcacgttcc
     9601 aaagtcactt tcaagttttc agcatctggg attactgagt cgtcaagtga agcataacga
     9661 cggtctgtgt gagctgagtg ctcatcatca cggtccatat ttggaggcaa tacatcgtat
     9721 gaacgacgcc agatgtgaac ttgctcatca ccaaattgtt cagcagcttc agctttgttt
     9781 ttaccagtca aaccaccgta gtgacgttcg ttcaagcgcc atgatttttc aactggaacc
     9841 cataattggt cagaagcttc aagagccaag ttagttgttt tgatagcacg tttcaatact
     9901 gaagtgtaag cttggtcaaa ttcgatacca gcttctttga tcaatttacc agcgtcaatc
     9961 gcttgttgtg tacctttttc agacaaatca acatcagccc aaccagtgaa aaggttagct
    10021 ttgttccatt cagactcacc gtggcgagca aaaaccaatt ttaccattag atggattctc
    10081 ctttaaattt tccgaggttt cccctcgttt atctattcta ttttacacaa tttttcacaa
    10141 aaaagctagt caaaaccaaa aaggaaagga ctgtttgaca atgtttggca atcacattgt
    10201 ttttggttgt ttctattcta tcttcattat ttaacctttt tttctgcgca tcgaataatt
    10261 tttgagttga ctcttttagt ttagtaaacg agtcattatt ttccatagct atgcctttga
    10321 aaccttcctc agctttagta aaataatcat caaaaatatt tttataaaga tctatataat
    10381 ctgcataagt gcgtattgat tcaatcgcat cgtctgttac aaccggtgta tcgaaagtga
    10441 ttgtttcttt tgaactattg gatgttgcta ctgtcttctc atcattactt gttgaagtat
    10501 tctttgaact ttatgttcca catgcaacta gaagagaaag agataataga gctactccta
    10561 taccgataaa cttttttgtt cttttcatgc taaaatacct tttattcttc tgatttgaat
    10621 ttcttcaatg ttgaaaggaa tagagatcct ccgataatag caaaaatccc cccaacccat
    10681 cctaagaacg gaatagaatg agactaactc caccagaaac aatcattagt acagacggag
    10741 ctgcacctac acgcttatct cctttatagt aaacaatagc aatgatacca agaacaagat
    10801 ttgcaatctt caaggcattc aagagtaagg ctacacctga aaaagcccct gcagtagctc
    10861 ctccattaag agttgtagca gctgtgttta ccgctattcc caacaagaca aaaggaccaa
    10921 taagaagtaa aattcctccc acaagaccga cgataccact a
//
LOCUS       DBERCA010000056        10750 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.056, whole genome shotgun sequence.
ACCESSION   DBERCA010000056 DBERCA010000000
VERSION     DBERCA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.056"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..277
                     /locus_tag="ACL719_001660"
     CDS             <1..277
                     /locus_tag="ACL719_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699592.1"
                     /translation="YLTTYNQKLSLRGMNYKLNHEELNNFDLLKKMFLKLMETDKIEN
                     DIFYLDGIKSILPLLSLDDRRNVLQHIQKQNNCPPPEIEELQRIIVN"
     gene            378..1256
                     /gene="truB"
                     /locus_tag="ACL719_001661"
     CDS             378..1256
                     /gene="truB"
                     /locus_tag="ACL719_001661"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGS0699593.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            1497..1799
                     /locus_tag="ACL719_001662"
     CDS             1497..1799
                     /locus_tag="ACL719_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS0699594.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            1907..2545
                     /gene="udk"
                     /locus_tag="ACL719_001663"
     CDS             1907..2545
                     /gene="udk"
                     /locus_tag="ACL719_001663"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS0699595.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            2673..4013
                     /gene="xseA"
                     /locus_tag="ACL719_001664"
     CDS             2673..4013
                     /gene="xseA"
                     /locus_tag="ACL719_001664"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGS0699596.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            3991..4203
                     /locus_tag="ACL719_001665"
     CDS             3991..4203
                     /locus_tag="ACL719_001665"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGS0699597.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIDLEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            4200..5075
                     /locus_tag="ACL719_001666"
     CDS             4200..5075
                     /locus_tag="ACL719_001666"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGS0699598.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            5068..5883
                     /locus_tag="ACL719_001667"
     CDS             5068..5883
                     /locus_tag="ACL719_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGS0699599.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     NLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            5876..6307
                     /locus_tag="ACL719_001668"
     CDS             5876..6307
                     /locus_tag="ACL719_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0699600.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            6314..7981
                     /gene="recN"
                     /locus_tag="ACL719_001669"
     CDS             6314..7981
                     /gene="recN"
                     /locus_tag="ACL719_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGS0699601.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMTEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            7983..8711
                     /locus_tag="ACL719_001670"
     CDS             7983..8711
                     /locus_tag="ACL719_001670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0699602.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            8790..10613
                     /gene="lepA"
                     /locus_tag="ACL719_001671"
     CDS             8790..10613
                     /gene="lepA"
                     /locus_tag="ACL719_001671"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGS0699603.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3197 a         1930 c         2609 g         3014 t
ORIGIN      
        1 ttacttaaca acttataacc agaaattatc tttacgaggg atgaattata aattgaatca
       61 tgaagaactt aacaattttg atttgttgaa aaaaatgttt ctgaaattaa tggagaccga
      121 taaaatagag aatgatattt tttatttaga tggcatcaaa agtatactcc cattattatc
      181 tttagatgac agacgaaatg tattacaaca tattcaaaaa cagaacaatt gtcctcctcc
      241 agagattgag gaattacaaa gaattatagt taattaaatt ttgtaaaaaa gagaataaaa
      301 ggtagacgac gcctcagtaa aaattggccc ggaaaaaagc gaagttcgaa gcactgaagg
      361 gggagtagaa agcaaaaatg aacggtatta tcaacttaaa aaaagaagca ggaatgacct
      421 cgcatgatgc ggtttttaaa ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg
      481 gaaccttgga tccggatgtg gtgggtgttt tgccgattgc ggttggcaag gcgacacgca
      541 tggtcgagtt tatgcaggac gagggtaaga tctatgaggg ggaaattact ctgggttatt
      601 ccacgacgac tgaggatgct agtggggaag tgatcgcaga aacccctgtt ttgtctccct
      661 tggatgaaaa gcttgttgat gaagcgattg ctagcttgac tgggcctatt acccagattc
      721 cccctatgta ttcggcagtt aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg
      781 gtcaggaagt ggagcgtcca gaacgtcagg tgaccattta tcaatttgag cgaacaagtc
      841 cgatttctta tgatggccaa cttgctcgat tcacttttcg tgtaaaatgc agtaaaggga
      901 cttacatccg tactttgtca gttgatttgg gtgaaaagct tggttatgcg gctcatatgt
      961 cccatttgac tcgtactagt gctgctggct tacaattaga agacgctctt gccttggagg
     1021 aaattgctga aaaagtagag gctgggcaat tagattttct ccatccttta gagattggga
     1081 caggtgacct tgtcaaagtt ttcctaactc cagaagaggc tacagaagtt cgctttggtc
     1141 gttttattga gctagaccaa acggacaaag aactggctgc ctttgaagat gataaattgt
     1201 tagccattct agaaaaacgg ggcaatctct ataagccaag gaaggttttt agctagatcg
     1261 tttaggaata aaaatcgggt gatagataac aattgcttga taaaacccca tactaatagt
     1321 agaatggttt tgggaattat aatattccaa ttgttgcgag ttgtaggtac tcaaataatc
     1381 tatatagaaa tttagaggtg tgaaatgaag caatttaaaa ttctttcaga taaatattta
     1441 gagtccatta caggttctgg tgggaactta ggcccaggat ttggtgtgat aattccatga
     1501 tgcgaaatga gtttcgagaa agggtggagc aacttcttca acaaaaagaa ataaatgaaa
     1561 atagtgagtt gagtcacctg tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat
     1621 accaatcggt catggccaat ttgagtcaag ggttgtcact ttacctcatg acgcatcatt
     1681 accaggcacc taagtctgtc attgattttg gtttatggat tgccaaagct cctagccagg
     1741 aaagagggag actggctttc ttgcagatgc ttgctcaaac cctacagggc tttcgttaaa
     1801 actgtcgaac atcagaggga agactctggt gttttttgct gatgagacta taaattttgt
     1861 aaaaaatgtg atagaataga cgacggataa aataacggag gatagcatgc aaaatagacc
     1921 aatcattatt ggagtgacag gtggttctgg tggtggtaaa accagtgttt caagagccat
     1981 tttatcgcat tttcctgatg aaaagatttc catgattgag catgattcat actacaagga
     2041 tcagtctcat ttgacctttg aagagcgtgt caaaaccaac tatgaccatc cttttgcctt
     2101 tgatacagac ttgatgatcg agcagattaa ggaattgttg gcagggcgtc cggtggacat
     2161 cccgacctac gactatacag agcatacacg gagtagcaag acctatcgtc aggagcctca
     2221 agatgtcttt atcgttgagg gcattttggt cttggaggac aagcgtctgc gcgatttgat
     2281 ggatatcaag atttttgtgg atacggatga cgatgtacgt attattcgtc gtatcaagcg
     2341 tgatatggag gagcgtggcc gtagccttga tagcgttatt aaccagtact taggtgtggt
     2401 caaaccaatg taccaccagt ttatcgagtc aactaagcgt tatgctgata tcgtcattcc
     2461 tgaaggggtt agcaataccg tggctatcga cctgttgacg accaagattg caaagatttt
     2521 ggaagaagct cgaaacagca aataatcaca tgtggaggcc ttgcctcctt tttctatttt
     2581 tccctcataa tggtacataa atggagattt ttaggcatat ttttggtata ataataccca
     2641 tgaaagagca agtaaatgaa tagtaggtgg agatggaaaa gtatttatcg gtaacaactt
     2701 tgaccaagta tctgaaaatg aaattcgata aagacccata cttggaacgg gtctatttaa
     2761 ctggtcaagt ttccaacttt cgtaaacgac ctactcacca atatttctcc ctaaaggatg
     2821 accatgcagt tattcaagcg accatctggt ctgggattta tcagaaatta gggtttgacc
     2881 tggaagaagg aatgaagatc aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta
     2941 gctactccat catcattgaa aaagctgagc ctgatggggt tggggcgctt gcgattcagt
     3001 ttgaacaact taagaaaaaa ttgacagaag aaggcctgtt tcaagaacgc ttcaagcaag
     3061 ctctgcccca attttctaag agaattggtg tagtaaccag ccgtagtgga gccgttattc
     3121 gagatattat cacgaccgtc agcaggcgat tcccaggtgt tgacatcctt ctttatccga
     3181 ccaaggttca aggtgaaggg gctgcggagg aaattgctag aaatattgcg cgtgctaatc
     3241 aacgggacga tttggatttg ctcattattg gtcgtggagg tggttctatt gaggatctct
     3301 gggcctttaa cgaagaaatt gtggtacgag ctatttttga atctcgtttg ccagttatct
     3361 ctagtgtggg gcatgagacg gatgtgacct tggcagattt tgtggcagat cgacgcgctg
     3421 caacgccaac agcggcggct gaactggcaa cacctgtgac caagttggat gtattagctc
     3481 atttgcaaaa tcaggaaaaa cggatggtaa cggcagtccg aaatgttcta tctaagaaac
     3541 aagaggcttt gaaaaaatgc agtcagtctg ttatctttag acaacctgag cgcttgtatg
     3601 acggttattt gcaacgcttg gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc
     3661 ggatttctga taacaaacaa ttagttcaag caagaactca tcaattagta caattatcac
     3721 ctgttaccaa aatccaacgc tatcaagacc gtttaggaca gttggacaag ctcttaggta
     3781 gccaaatggc gttagtttat gacgccaagg ttgctgaggc caagcgactt tcggaagctt
     3841 tgctcatgtt ggatactagc cgaatcgtgg cgcgtggtta tgctattgtc aaaaaagaag
     3901 agtccgttgt agattcggtt gagagtttga agaaaaaaga ccaagtaacg cttttgatgc
     3961 gagatggtca agtagaatta gaggttaaag atgtcaaaac aaaagaaatt tgaggaaaat
     4021 ctagcagaac tggaaaccat tgtccaaagt ttggaaaatg gtgaaattga tctggaagat
     4081 gcgattactg cctttcaaaa gggcatggtc ttgtcaaaag agctccaagc tacgctggac
     4141 aaggctgaaa agaccttggt caaggtcatg caagaagacg gaacagaaag tgattttgaa
     4201 tgaaaaagca agaaaaatta gctcttgtcg agtcggcttt ggaagatttt tatggagacc
     4261 agcagtttgc ctctagttta cgggagtctg ttctctattc tattcatgct ggtggcaagc
     4321 gtattcggcc ttttctcttg ttagaagttc tggaagcctt gcaggttacc atcaaacctg
     4381 ctcacgcgca ggtagctact gccttggaga tgattcatac agggagcttg attcacgatg
     4441 accttcctgc tatggatgat gacgattatc gaagagggcg gttaaccaat cacaagaaat
     4501 tcggtgaagc tatggccatt ttggctggag atgccttatt cttagactca tatgccttga
     4561 ttgcgcaggc agatttgcca agtcagatta aggtggactt gattgccaac ttatcccttg
     4621 cttcaggtag tctgggtatg gtggcagggc aagttttgga tatggagggc gaacaccagc
     4681 acttgtctct ggaagaactt cagactattc atgccaataa gactgggaag ttactagcct
     4741 atcccttcca agcggcagct attatagctg aattgtcacc tgaaatgcag gtgaagctga
     4801 aaactgtggg tgaattgatt ggacttgctt ttcaagtcag agatgatgta ctggatgtga
     4861 cagctagttt tgaggaaatc ggcaagacac ctcaaaagga tctgcaggca gaaaaatcaa
     4921 cctatcctgc cctgttgggc ttggaagagt ccattgcctt ttgtaaccaa accctggatc
     4981 aagctaatga aaaattagaa gaaattgccc agcagcttcc ctttgaaaca gaatcgattg
     5041 taagtgtagt agaaagtttg agaatcaatg gctaaggaaa gagtggatgt actagcttat
     5101 aaacaggggt tgtttgaaac gagagagcag gccaagcgag gtgtgatggc tggcctagtc
     5161 gtagcagtcc ttaatggaga acggtttgac aagccaggag agaaaattcc agatgacacc
     5221 gaattaaaac tcaaggggga gaaactcaag tatgtcagcc gtggtggttt gaaactggaa
     5281 aaggccttgc aggtctttga tttgtcggtg gatggcgcga ctacgattga tatcggggcc
     5341 tctactggag gttttaccga tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc
     5401 gatgttggta ccaatcagtt ggcttggaaa ttacgccaag acccacgagt tgtcagcatg
     5461 gagcagttca atttccgcta tgctgaaaag actgatttcg agcaggagcc gagctttgcc
     5521 agtattgatg tgagtttcat ttcccttaat ctgattttgc cagccttgca ccgtgtcttg
     5581 gctgatcaag gtcaggtggt agcacttgtc aaacctcagt ttgaggcagg acgtgagcag
     5641 attgggaaaa atggaattat tcgagatgct aaggttcatc agaatgtcct tgaatctgta
     5701 acagctatgg cagtagaggt aggtttttca gtccttggct tggacttttc tcccatccaa
     5761 ggtggacatg gaaatattga atttttagcg tatttgaaaa aagaaaagtc agcaagcaat
     5821 cagattcttg ctgagattaa agaagcagta gagagggcgc atagtcaatt caaaaatgaa
     5881 taaaaaagaa agacttgaaa aaattagaag attggtgaca gattatcaaa tcggcacgca
     5941 agaagaaatt gtagaacatt tgaaagaagc aggtatcact gccactcagg cgacggtatc
     6001 ccgagatatc aaagagttag gtattgtcaa aattcctttg agagacaaca cttatgtcta
     6061 cgaattacca aaatcaatcg taaaaagtct gcaactggct gaagacaata tcgaatcggc
     6121 tgaattgatg gataagatga tcaatctcca agttattcca ggaaatacgg cttttgtaaa
     6181 agctcagtta atcgagactt ttgcagacaa gatttttagc tgtttgactg atgatagctc
     6241 gattttagtc attgccagaa gtggaagtct agcagaggaa atctttgaac aagtaaaaaa
     6301 ttggtaggtc tatatgttac ttgaaatttc gataaaaaac tttgccatta ttgaggctat
     6361 ttccctcaat tttgaaaagg ggatgactgt cctgactggt gaaacgggtg cagggaagtc
     6421 gattatcatt gatgccatga atatgatgtt gggagctcgt gcgacgacag atgttattcg
     6481 tcatggtgca ccaaaggcag agattgaggg gcttttctca gttgagaata gtcgtctttt
     6541 acaggaaatt tttgatgagc aaggtttaga attaggtgat gaaattatca tccgtcggga
     6601 aatcttgcaa aatggtcgta gtatcagccg tgtaaatggt cagatggtta atctgtctgt
     6661 tttgcgagct attgggcaac atcttgtaga tattcatggt cagcatgacc atgaggagtt
     6721 aatgcgtccc caactgcata tccagatgtt ggatgaattt ggtgatgccg ctttttggga
     6781 cttgaaagaa acctatcaaa cgagttttga tgcctatcgg aaaatgcgta agcaggttct
     6841 ggaagtcaag aaaaaccaac aggaacacaa ggcacgtatc gaaatgttgg aatttcaaat
     6901 gacagagatt gaggcagcaa acttgcaggc tggagaagac ttggctctca atcaagaacg
     6961 agataaactc ctcaaccaca aaaatattgc ggatacactg accaatgcct acagtatgtt
     7021 ggacaatgaa gatttttcaa gtctggccaa tgttcgttca gctatgaatg acatggaaag
     7081 tgtcgaagag tatgatcctg aataccgtga aatttcaagc tctctgtctg agacctacta
     7141 tgttttagaa gacattagca aacgtttgga agctatcatt gaggaccttg attttgatgg
     7201 caatcgcctc atgcaggttg agaatcgttt ggacctcctt catactatta ctcgtaagta
     7261 tggtgggact gttgatgatg ttttgcttta ttttgccaag attacggaag aatacaatct
     7321 cttgacaggc aataatcttt cgtctgagga catggaagca gagcttaaga agttggaagt
     7381 caatcttgtc aatttggcag gtcaacttgc ttctgctcgt cataatttgg ctcagcaact
     7441 cgaagctgag attaaacaag aactgcaaga tctttatatg gaaaaagccc agtttcaggt
     7501 tcgttttagt aagggaaaat tcagtcgtga gggaaatgaa atggttgagt tttatatttc
     7561 aaccaaccct ggagaagact ttaaaccctt ggttaaggtt gcttctggag gggaattatc
     7621 tcgtctcatg ttagccatta agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt
     7681 ctttgatgag gtggatacgg gagtttcagg tcgtgttgct caagctattg ctcagaaaat
     7741 tcataaaatt ggtcagtatg gtcaggtttt ggctatctcc catttgccac aagtaattgc
     7801 gattgcagat tatcaattct ttattgagaa gattagtaat gaccattcaa cggtttcgac
     7861 tgttcgtctc ttgacagtcg aagagcgagt ggaggaagtt gccaagatgt tggcaggtga
     7921 tgatgtgaca gaagcagccc tgacgcaagc cagagaattg ttgagaaaca gggagaaata
     7981 agatgacaga ttattatgta attggagatg ttcacggaaa agctgggatg ctggaagacc
     8041 ttcttaaaac ctgggatggt cagacccagt tgctctttct aggtgatttg attgacagag
     8101 gtgaggatag tcaccgtgtt cttgaaatgg tcaaggactt ggtggacaat caaggggcta
     8161 tctgtttgtc tggaaaccac gagtatatgt ttttgacttg gctagatgac ccagaagaaa
     8221 gttatgacca ttatcgtcgc aatggtggag atacaaccat taactctatc ctaggtcgtc
     8281 ccttggatgc accagttgat ggagtagaag atgccaagcg ggttgccgct gaagcggcag
     8341 acttggtcga attcattcgt caaatgccat ttgtagtaga gacagacaag tatatctttg
     8401 ttcacgcagg tattgatttg accttggacg actggcatga aacaacagat tataaaaaag
     8461 tatggcttag aaaaccattc cacggagctg aaaatcatac tggaaaaacc attgtcttcg
     8521 gtcatacacc ggtctatggt ttgttacagc aagaacgcgg tacatctgag ctttggacta
     8581 cagaagatgg caagattgga atggatggag gagctgttta tggtggtgtc cttcacggga
     8641 ttgtctttac agaccaagga atgacagaac accactttat cgaaaatgac ggctttgtcg
     8701 ctgaagatta gtactcttag cagggtatgg tcttgtcaaa atatcaaaaa caatttataa
     8761 taaatagata ccctgaaagg aagagaatca tgaacttaga agaattgaaa aaacgacagg
     8821 agaagatccg taacttctct attatcgccc atattgacca cggaaaatca actctagcag
     8881 accgcatttt ggaaaaaaca gagacggttt caagtcgtga aatgcaggcc cagcttttgg
     8941 atagcatgga gctagagcgg gaacgtggaa ttactattaa gttgaatgcc atcgagttga
     9001 attacactgc aaaagatggg gaaacttata ttttccactt gattgacaca ccagggcacg
     9061 ttgactttac ctatgaagtt tcacgttcgc tagctgcctg tgagggtgct attttggtgg
     9121 tcgatgcggc tcaaggaatt gaggctcaaa ctcttgccaa cgtttatctg gctttggata
     9181 atgatttgga aatcatgcct atcattaata aaattgacct gccggctgca gatccggagc
     9241 gcgtgcgtac agagattgaa gatgtgattg gtttggatgc cagtgaagca gttttggctt
     9301 ctgccaaggc tggtattggg attgaagaaa tcctcgagca aattgtagaa aaagtaccag
     9361 caccaacggg tgatgtgacg gcgccactta aggccttgat tttcgactct gtttacgatg
     9421 cttaccgtgg ggttatcctc caagtacgtg tcatggacgg agtggtcaaa cctggtgata
     9481 agattcagct catgagcaat agtaagacct ttgatgtgac cgaagtcggt atttttacac
     9541 caaaagcggt tggtcgtgat ttccttgcga ctggtgatgt tggttacatt gcggcgtcta
     9601 tcaagacggt tcaggatact cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg
     9661 cagaaccatt acatggttat aagcagatga atcctatggt ctttgcgggt ctctacccaa
     9721 tcgaatcaaa caagtacaat gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg
     9781 ctagtcttca gtttgaacca gaaacatctc aggcacttgg atttggtttt cgttgtggat
     9841 ttcttggact tctccatatg gatgtgatcc aggaacgttt agagcgtgag ttcaatattg
     9901 acctcatcat gacagctccg tctgttattt acaaagttaa tttgaccgac ggtgagtcta
     9961 tggatgtgtc taacccatct gagtttccag acccaactaa gattgcgacc attgaagagc
    10021 cttatgtcaa ggcgcaaatc atggtaccac aggagttcgt cggagcagta atggagctag
    10081 ctcagcgtaa gcgtggggac tttgtgacta tggactatat tgatgataac cgtgtcaatg
    10141 ttatctatca aattcctctt gctgaaattg tctttgactt ctttgataaa cttaagtctt
    10201 cgacacgtgg ttatgcaagc tttgactacg aattgtcaga atatcgccca tctaagctgg
    10261 tgaaaatgga tatccttctc aatggagata aggtggatgc cctcagcttt atcgttcaca
    10321 aggactttgc ctacgaacgt gggaaactca tcgttgataa actcaagaaa atcatccctc
    10381 gccaacaatt tgaagttcca atccaagcgg ctattggaca caagattgtc gctcgtactg
    10441 atatcaaggc ccttcgtaag aacgtacttg ctaaatgtta tggtggtgac gtttctcgta
    10501 agcgtaaact ccttgaaaaa caaaaagctg gtaagaaacg catgaaatcc atcggatcag
    10561 ttgaagttcc gcaagaagcc ttcctcagcg tcttgagtat ggatgaagaa taagttatca
    10621 gaaatgcctt tccttgttca caggaaattt atataagaat caatagtaaa gcaggcttag
    10681 atggtctgct tttttagttt ttatactctt cgaaaatctc ttcaaaccac gtcagcgtcg
    10741 ccttaccgta
//
LOCUS       DBERCA010000057        10504 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.057, whole genome shotgun sequence.
ACCESSION   DBERCA010000057 DBERCA010000000
VERSION     DBERCA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.057"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(17..1441)
                     /locus_tag="ACL719_001672"
     CDS             complement(17..1441)
                     /locus_tag="ACL719_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS0699604.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(1986..4265)
                     /gene="pulA"
                     /locus_tag="ACL719_001673"
     CDS             complement(1986..4265)
                     /gene="pulA"
                     /locus_tag="ACL719_001673"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGS0699605.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4377..6335)
                     /gene="ligA"
                     /locus_tag="ACL719_001674"
     CDS             complement(4377..6335)
                     /gene="ligA"
                     /locus_tag="ACL719_001674"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGS0699606.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASKLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6428..7606)
                     /locus_tag="ACL719_001675"
     CDS             complement(6428..7606)
                     /locus_tag="ACL719_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0699607.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7686..8549)
                     /locus_tag="ACL719_001676"
     CDS             complement(7686..8549)
                     /locus_tag="ACL719_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS0699608.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8552..10453)
                     /locus_tag="ACL719_001677"
     CDS             complement(8552..10453)
                     /locus_tag="ACL719_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0699609.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3135 a         2327 c         1901 g         3141 t
ORIGIN      
        1 ttcctgattt tacacgttat ttcacatcaa aaacaatgga tttgacattt gtcatcgctt
       61 caatgctata tttaattcct tgcactccag ctccagaacc tttgacacca aggaatggga
      121 aattatctgg accacgctgg gttttattat taatgtggac tgtacctact tcaagttttt
      181 cagcaatttc aaaggctttt ttgaaatcat ttgtaaagac tgatgattga aggccgaatt
      241 cagattcgtt ggcaaaggca atagcttcct ctacactagc cacacgaatg attggtaaaa
      301 caggaccaaa tggctcttcc catgccactt tcatatcttt tgtaacttgg tcaaaaagca
      361 ctggccagag gagattgccc tcacgtttga ttggtgtaag agcctgagcc tctttttctt
      421 gtgcatcctc aatcaagccc caaatgaagt cggctgaagc attgtcaata acaggtgtaa
      481 tatcagcatt gtcaaatgga tcaccaactg ttaatttaga aacttcttcc tgaagcaaag
      541 tagctaattt atctgctaca ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt
      601 gtcctgagta gctaaaggct cccgcaacaa tttgcttggc agcatgttcc aaatctgcat
      661 cttctagtac aagagctgca tctttcccac caagttccaa catgatagga cgcataccag
      721 ctaaacgacc aatacgttct ccaatagggg ttgaacctgt aaagttgatg aagttgactt
      781 ctttgtgctc aatgatataa tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa
      841 cacctgccgg aatccctgct tcttcaaatg ctttagccaa caagagtcca gaaatggaac
      901 cttgtgttgg tggcttaaac atgaccacat tccctgcaat caaggcaggt gcaattttag
      961 aagcagataa attaactgga taattaaagg gagcaatcgc tagcacgata ccaactggtt
     1021 cacgacggac aacagccagt ttgtttttac ttgctgcctc aaaaccacca ccttccattg
     1081 cttgtccagt gatacggaga ccttcctcag cagcataacg aatcaagtct gctgtacgca
     1141 ctacttctcc aattgctgct ttaatccctt ttgctacttc tttggcaagg atagtaccaa
     1201 ttttttcctt atcgcgttct aaaatagctg ctgttttatg caaataagcc gcacgttcaa
     1261 ttgctgataa agctcgccat gctggcaggg ctgcacgcgc agcttgcata gcctcatcag
     1321 cttcagtctg agtcatggct ggaactgtac ccaattcttc ttgattgatt ggtgaataaa
     1381 tcgtaatttc ttgttcagat gatttccatt ttccatttac taaattctga tatcttgtca
     1441 aattcttgtc ctcctgaaaa tgattaagat atattttacc aaattttcag aaaatcacaa
     1501 ccctttttgc aaaatttttg agattatttt cacaagcttg atttttcaaa gtatactcaa
     1561 taaaaataaa aaaaatccac tacatcaagg cgagactaat gtggtttgaa gaaattttcg
     1621 aagagcgtga atgagtatca tctatagtaa aataaaaaaa ctgaacaatt tggttgggga
     1681 cagtcaaacc aatttctcac aatgttttag aaacaagggt gtgctattcc aatttcaggc
     1741 tactataact gtcatagatt gctgaaacaa agtctaggta aaagtcttca taataaaaag
     1801 accccctatc aagtgttcaa aaactttgat aggaggtctt gttttgtgaa aatatttatc
     1861 aaattttcta tacaagtgag ctgttagcca ggttctttct attctttcaa tttcaatgaa
     1921 tggatttttt accaatactc ataactggga atttgtctgt gtaaaaatag cgagatagat
     1981 ggtatttata aaacactcaa gacagctaga ctaatatcat ttaaaacatt atcttctttt
     2041 gagcgactgt tggttaccaa catagctaaa tttcctgcat tttcaaattg atagggttct
     2101 gatttagcat tcacaactac caagaggtgt tctttgccgt gaacttcata gataaggcag
     2161 ccgctatgtt caatcgcaga atgcacaaag acatgatggt aaatttcatc atagctagag
     2221 taagaaaagg caccagtttt tgtcttcaat cggatgactt gacggataaa ctcaatactg
     2281 tcttgacgct cattaatcaa gttccagttc acttggttca cactgtcagg agcattatag
     2341 ctattcatcg cacgctctct atcatcatgg gtcaactcac cattttcacc agtcgcaacc
     2401 agtttggtac gaccaaattc ttgaccgatt tccataaagg ccatcccctg catgagcaga
     2461 ttcatggctg tggcagtttc gaccttgcgc atgatttgct ctgaactttg gtctggatga
     2521 agggttgcca ataaatcgtg aagattgtaa ttgtcatggg cttctacata gttaagcacc
     2581 tgatttggat gtgtataggt tcctaattca cgacttccta ggattgcttt agctagaatt
     2641 ggctctgtcg cagcaccact gacaaaacct gacttgatag caccataaac ttctccccct
     2701 ttgacagcat cgcgctgatt gtcattaaag aaaccaatat ttggcatctg gtaggcattg
     2761 tccttcttgg ccttatcata aggggtaaga cctgttccca tatcccatcc ttctccatag
     2821 aggataatgt tggagtcgat ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca
     2881 tcatgaatcc ccatcaagtc aaaacggaag ccgtcaatat tatattcctg cacccagtat
     2941 agaagagaat caatcatata cttgcgaaac atttcgtgtt cactggctgt ttcatttcca
     3001 acacccgttc cattctggaa ggtaccatct ggattcatac gataatagta atcagggact
     3061 gttgtttgga atggtgcatc aacaactgag aaggtatggt tatagactac atccataatg
     3121 actccaatac ccgcatcgtg ataagcttga accatcacct tcaaatcacg aatgacctga
     3181 gctggatcat ctggattagt tgaaaaacta gtttctggcg cgttatagtt ttgtggatca
     3241 taaccccagt tgtaggttac atttccatcc tcatcgtatt ctttatgacg gtctgcaatt
     3301 ggttgcaatt gaacataatt gtagcccagc ttcttgatgt aatcaaaagc agttgactgg
     3361 ccgtattggt taactgttcc agcctgagca gcacccaaga aagttcctcg aagatgttca
     3421 tctacacccg atgtaggtga tttagtcaaa tcacgaatgt gcatttcaca gataactgcc
     3481 ttacatggat tttccaagcg ccaagtagcc tccgaaccgt gcttaacctc gaagttttca
     3541 acttgctttt ctacatggct cagaatagct gaacgtttgc catcagggct ggtcgcgatt
     3601 gtataaggat cacgtgtcag tgtttggtga tgagggaatt ggacttgata ctgataagtc
     3661 ttacctacca aatcttcttc aacatccaaa ctccagacac cgattgtatt gtccttatga
     3721 ttataagagt agctattgcc tcttttcatc tcaaaagtct tccaaacggg tgcatcatta
     3781 gcagctgatt cataaacgac aacttgcact tctgtcgctg taggtgacca gagggaaaaa
     3841 tgagcctgat tgtcctctac acggcaaccc aattctcctt ggtaacccca atgatgatca
     3901 aaactagcac tgttaatggc cttatcaaag gcaaaaggat tttgattttt atagaaagga
     3961 ctggcaatag caggattttc agagtaataa atcctatcat cgccttccaa aatccagacc
     4021 tctgtcaata ggggatagtg attaaaacgg atagaatatt ctttactagt ttgacctgta
     4081 tgaaccacaa aattcaagct ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa
     4141 gctccaaaat aatcttcttt gtaggttagc aaatcaattc gttgatcctg actttttaca
     4201 aaggagcaag tgtcatattc tccattctta cgatggtaat gaatgcgcat agggtagtta
     4261 tacatttttt atttttcctt tttactttgt ttctatttca ctaataaatt tttgtcaatc
     4321 tcgtctcaat taacagacat agtcatattc tctaaactct gtttttaaac gatccattac
     4381 aaactttcta gccatgcctc atctctgacc tggataccaa gttcttgtgc tttttgcagt
     4441 ttacttccag cgtctgcacc taccacgacg aggtcggtct ttttagaaac actacctgtc
     4501 actttggcac ccagactttc gagtttactt ttagcttctg agcgcttgag tcgttccaat
     4561 tttcctgtca ataccacggt caaacctgac aaggccgcat ccgctactac cgtctgtcct
     4621 ttatagtcca gattgacccc agtttctttc aattctctga gcagaatttc agagccttct
     4681 gtcgcaaaat aagtctgaag acttttggca atcacgccac ctagactttc aatactagcc
     4741 acttcctctg aatctgcctg atacagattt tcaattgaat ggaaatattg aagtaaaagc
     4801 ttactagcct tgcttccgac atgacgaatt cccaaaccaa ataagagctt ctcggcagaa
     4861 ttttcctttg atgcttggat agcctgatac agtttagcag cggacttttc cttaaccccc
     4921 tctaaaagga ggaaatcctc ttcttgcaaa cgataaatat ccgccacatc cttgactaaa
     4981 ttagcagcaa aaagcttctc aacaatagat ggaccaaggc ctgtaatatt catagcatca
     5041 cgagaagcaa agtgaatcaa gccttccatg atttgagcag ggcaacgcgg attgatacaa
     5101 cgtagggcca cttcatcttc aaagtgcaac aagtcagagt tacaacttgg acagtttgta
     5161 gggatatcta gtttttcttc agaaacccgt ttggactcta ccacacgtaa aacggcaggg
     5221 atgatgtcac cagccttata tacaatgacc gtatcgtctt ttcggatatc tttttcagca
     5281 atataatcta cattgtgcag ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt
     5341 gttagattag cagttggagt tacaacaccg gtacggccaa ctgtccagtc aactgataag
     5401 agttgagctt ctttttcttc agcagggaac ttgtaggcta ctgcccactt tggagcctta
     5461 acggtaaaac caagttcttc ttgacttgct aggtcgttga ccttgattac cactccatca
     5521 atatcgtaag gcagattttc ccgttcctgt cctacttctt ggataaaatt ccagatttca
     5581 tctatgtttt cagccaagat tcgcttagga ttgaccacaa aacctagttg ttctaggtac
     5641 ttcaaaccct tttcttggct atcacgagtt gaagggctgg cttcttgata gagaaacgtt
     5701 gcaagattac gcttggcaac tactgctgta tccaactgac gcagagttcc tgctgccgca
     5761 ttacgaggat tagcaaattc aggctctcca ttttcttggc gcacttggtt aacttggtca
     5821 aaggaagcgc gtggcatgta acattcccca cgaactgtga tatctagttc ttctggcaaa
     5881 gtcaaaggga tgtccttaac acgcttgagg ttttctgtga tattttcacc aattgaacca
     5941 tctccacgtg ttaccccagc aaccaaaatc cccttttcat aagtcagcga gatagataag
     6001 ccatcgattt tcagctcaca aatataggtc ggatgagcca cttccttacg aacacgcgca
     6061 tcaaaagcat ctagctcctc acgtgaaaaa gcatcctgca aactataaag aggatactga
     6121 tgactgtatt tttcaaaacc atctaaaacc ttgccaccaa cacgatgagt cggactgtct
     6181 gctagcactt gctctggata agcagtttct aactcgacca actcacggta aaggcggtca
     6241 tactcactgt ctgaaaccga gggattatcg ctggtatagt actcagtcgc atagcgattg
     6301 agcaaagcga ctaactcatt cattctttta ttcataagac cattttacca taaaacaagc
     6361 cctcctcaca aacgagaagg acggaaaaaa cacttagttt gaaattattt ttgaaactca
     6421 agcaacctta tatcaatttt tcaaaatgag ttcgaacata aataaacgat atacaagaca
     6481 agatgataac accacttcca attatcagga aagaagagag atgtacactt ggcaagactg
     6541 tcataaatcc ttttgcaata ggcataaata gaatagctaa ggtaaaaatt gtactcagta
     6601 ctctcccaag aaattcgctc tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa
     6661 aaatcgtcat aaacaattca caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg
     6721 gtaatcccat cataaaaact ccgacacctg tcaaagccag taaaatcaaa agattataaa
     6781 tattagcttt aattttacta gctagaagag ccccaatgat ggaaccaata gcccccatag
     6841 ttaaaatact tgcataggct ccttctgatc cgtaaagctg attcgaaaag ggaagtagaa
     6901 attcaaaagc tgcaaaaaag aaattaacgc tggaagctac cagcaaaagg aagaaaattt
     6961 cttgctgatg ccagatatag tgtaacccat ccttgatatc tacaaaaata tctctcccag
     7021 taaaagcctt tttctcttga acttttgctt cctcttttgg aaggaaagcc actagaacaa
     7081 aagcaatgaa aaaagtcagc gagtctagca gtagcgtcat atggagactt gcaaactgta
     7141 aaacaaggaa ggaaagaaca ggagagctaa cacctacaac ctgcaaaacc agctctaggc
     7201 gagaattata gatcacaatc tcatctttct ccaccacttc agttatgata gctttattgg
     7261 ctgtgcgaga aaaggcaaaa gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa
     7321 tcatccagct atcattcctt atgaaagaaa tagccagaca aagaatccca caaacaagat
     7381 ctgccgtcat taaaatctta cgacgagaaa aacggtctga aataactccg ccaaagggat
     7441 tgacgagaat agatgtgacg agctcagaaa tctgatacat tcctaaaact gtctgtccta
     7501 tagtccccat agaagccaac cagacactat ttccataatc atagagcata tttcccattt
     7561 tattgatagc cccacggcta atcaactgca ctgcatagcg attcatatta aagctcctct
     7621 caaattttga aactattgta tcaaaaccga aaggagcttt ttattttttc ccttatttgg
     7681 gaaaattaac ttttgacaaa tttttcgtag tgttcctgat aataggctac ttgctctgga
     7741 agacctaaca catcaaaaat atgtatggcc tcttgcatct gcttacagcc ttctttacac
     7801 tgtccttttt gatataaggc aaaacctttt aaataatgga aaacattacg ctcataaagc
     7861 ttaatacctt tgtcaataat cttctctgta taagcctcaa aatagttggc attataaaaa
     7921 gaagaatgct ctaaacaatg ctggtaacaa ttgagggcca aaatcaaaac taatctctta
     7981 tggcgactaa tctcttggta aaattcctcc ctctccataa cttctctacc aatccgagtg
     8041 acatagtcta catcgtagaa actatagagg ttaccgaaaa gaatcaactc atacatggtc
     8101 cattcttctg ttttgaagag ataatctgct accttatcca aatcatcctg cttcatatca
     8161 taactcgcat ctctttgaca aatcagacct tgtagcaaaa tccagttcag ctcaaaataa
     8221 aggggagtcg tcgaactctt agacttttca agttgttctc tttgaagctt ttgaaaacct
     8281 gcaatatcgt ttgaatagta aagtgggata atctgtgcca tcatagacac atgttcatga
     8341 ttgtgaaaat tccttgcctt atccatgaaa ttttcgattg ttacatgaat gttatccaaa
     8401 atctcaaaga aacgggagac tgccaggtca gactccccaa gctcaaagcg agataactga
     8461 gaggtagagc aggattcgcc tgctgcttcc tttaaagaat aatttccact tgttcgaaat
     8521 tcacgaaata cttttccaag atgttccatc tttacacctg atctgataat tcttcccact
     8581 caagcatagc ttcttcctga cgatggctga ttttgtccag ctcagcctgt aattccatga
     8641 gtttgtcggc atcgtttgtt tccaacattt gttcagaaat ggcttggctt tgactttcta
     8701 gctcttcaat ttcagcttct agactttcga tttgtcgcat gagtttgcga acttcttttt
     8761 gactttcttt ctgggcctga tagtcattga ctggacttgc ttcctttgct tgattgctag
     8821 ttgaagcttc ctcagtctga ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt
     8881 aatctccaag gtagagagtt gaaccattct cagacaattc caaaacatga gttgccacac
     8941 gattgataaa gtaacgatca tgactgacaa aaagcaaggt accatcaaag tcaatcaaag
     9001 cattttccaa cacttccttg ctatcaatat ccaagtggtt ggtcggctcg tcaaggatca
     9061 ggaagttgtt gttttccata gacaatttag ctaaaagcaa acgagctttt tcgccaccag
     9121 atagcatgcc gactgatttt ttaacatcat ctcctgagaa aaggaaggct ccaagacggt
     9181 tgcggatttc aacttctggt gtcagtttga aatcattcca gagttcatcc agcaccgtat
     9241 tacttggtgt cagcttgctt tgggtttggt catagtaacc aacctcaaca ttagcgccaa
     9301 agcgcttttc acctttgata aaaggaatct ggtccacaat agacttgata aaggttgact
     9361 tgccgatacc atttggacca acgatagcga cagcattcat cttacgaaga tctaggttga
     9421 taggttgtga caagacttcc ccgtcataac caatagcagt attttcaaca gtcaaaacaa
     9481 cattgcccga cgttttttca gactggaagg tcatgttggc tgctttcttg ccagcttcag
     9541 gcttgtccaa acgctccatt ttttctagtt gtttacggcg agattgagca cgtttagtcg
     9601 ttgaagcacg aactagattg cgattgacaa agtcttccag agcagcgatt tccttctgtt
     9661 gcttttcata gttttttgcc tcagtaacta gcttttgctc cttcaattcg acaaaacgag
     9721 agtaattccc cacatagcga tccaaggaat gcttggtcaa atctagcgta attgtcgcaa
     9781 ccttgtccaa gaaataacgg tcgtggctga cgataatgag ggcaccgcta tagtttacca
     9841 agtaattctc tagccaggcg atggtttcaa tatccaagtg gttagttggc tcgtccaaga
     9901 ccaagagatt gggcttttca aggagcattt tggcaagtgc caaacgagta ttttgaccac
     9961 cagaaagctc agcaattttc atctgccaca tagactcgtc aaacttgaat ccattcaaaa
    10021 tcgctcgaat atcagcttca taggtaaagc cacctgcttg gcgaaaattc tcagataagc
    10081 ggtcataatc tgacatcagt ttatccaaat cctcaccaga cttttcaccc atctccagct
    10141 ccatctgacg cagttgtctc tccgtccgac gcaaattatt aaagacatga agcatttcat
    10201 cgtagatggt attttcagac tcaaaacggc tatcttgggc taggtaagac agagaaatat
    10261 cttttttctt attgatttct ccgctagttg gctcctcttc tccaactaaa atcttcaaaa
    10321 gagtagactt acctgcacca tttttcccaa caagggcaat ccgatctcgt tcatcaacct
    10381 gcaggttgat attatcgaaa agaacctctc ctgcaaaaga acgttcaatt ttattagctt
    10441 gtaaaataat cataagagta gtatagcatg tttccctaag gcattcaaga taatcgtaag
    10501 tctt
//
LOCUS       DBERCA010000058        10364 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.058, whole genome shotgun sequence.
ACCESSION   DBERCA010000058 DBERCA010000000
VERSION     DBERCA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.058"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(412..804)
                     /gene="rpsI"
                     /locus_tag="ACL719_001678"
     CDS             complement(412..804)
                     /gene="rpsI"
                     /locus_tag="ACL719_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGS0699610.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(824..1270)
                     /gene="rplM"
                     /locus_tag="ACL719_001679"
     CDS             complement(824..1270)
                     /gene="rplM"
                     /locus_tag="ACL719_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGS0699611.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1627..1944
                     /locus_tag="ACL719_001680"
     CDS             1627..1944
                     /locus_tag="ACL719_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGS0699612.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMYGRETILLPEEN
                     DLF"
     gene            complement(1989..2801)
                     /gene="folK"
                     /locus_tag="ACL719_001681"
     CDS             complement(1989..2801)
                     /gene="folK"
                     /locus_tag="ACL719_001681"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGS0699613.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYDALKK"
     gene            complement(2844..3398)
                     /gene="folE"
                     /locus_tag="ACL719_001682"
     CDS             complement(2844..3398)
                     /gene="folE"
                     /locus_tag="ACL719_001682"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGS0699614.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3379..4701)
                     /locus_tag="ACL719_001683"
     CDS             complement(3379..4701)
                     /locus_tag="ACL719_001683"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS0699615.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHFKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4703..5677)
                     /gene="folP"
                     /locus_tag="ACL719_001684"
     CDS             complement(4703..5677)
                     /gene="folP"
                     /locus_tag="ACL719_001684"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGS0699616.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5772..6479)
                     /locus_tag="ACL719_001685"
     CDS             complement(5772..6479)
                     /locus_tag="ACL719_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0699617.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6532..7950)
                     /locus_tag="ACL719_001686"
     CDS             complement(6532..7950)
                     /locus_tag="ACL719_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGS0699618.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8140..8952
                     /locus_tag="ACL719_001687"
     CDS             8140..8952
                     /locus_tag="ACL719_001687"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0699619.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9291..10310
                     /gene="adhP"
                     /locus_tag="ACL719_001688"
     CDS             9291..10310
                     /gene="adhP"
                     /locus_tag="ACL719_001688"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGS0699620.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3058 a         2339 c         2008 g         2959 t
ORIGIN      
        1 cccaaactct tcacacaaga gttctaactt acccattcta tggaatcttg cattatccat
       61 aatatagtag attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg
      121 atttgactat cctcggtttg tcctattctt atttcacctt actataaaaa taaggtaaac
      181 ttttcaaaaa aacacgaaaa aagcacccat agggcaaacc tttgagtgct ttgaaacatt
      241 gatataattg tattgattag cgttttgagg caactgacct tgtcaggttg cagccacatt
      301 tgtaagcaac taaagtcgca acaactgtgt caattgcacc aatttagtaa gaaagtataa
      361 aaaaagaaca ccccgaaagg tgctctgtat aagtatagta attctttcga attaacgttt
      421 actaaattgt gatgctttac gagctttctt aagacctggt ttcttacgtt caactttacg
      481 tgagtcacgt gtaagaagtc ctgcgcgttt caatgaatcg cggaagtctg ggtctacttg
      541 aagaagggca cgagcgatac cgtgacggat agctcctgat tgaccagcgt atccaccacc
      601 tacaacgtta acgaaaacgt cgtatgaacc tacagttgaa gtaactgcga atggttggtt
      661 gatgacaaga cgaaggtcag catgtgggat gtactcttca acatcttttt tgttaacagt
      721 gattttacca gttcctggaa caaggcgaac gcgtgcaaca gcgtttttac gacgtccagt
      781 acctgcatat tgtgcttgtg acatacttta tttgttcctt tccttagata agtcctgaaa
      841 tgtcaagaac ttctggttgt tgtgcagcat gagtgtgctc agctccaaca aatactttca
      901 acttcatacc ttgagcgcgt ccaagtgtat tgtgtggaag cataccttta actgatttct
      961 caatcaaacg tactgcattt ttagaacgaa gttcacctgc agagatttgt ttcaatccac
     1021 ctgggtggtt tgagtgagtg tagtagattt tatcagttgc ttttttacca gtcaatttaa
     1081 ctttttcagc attgataaca atcacgaagt cacctgtatc agtgtgtggt gtaaatgttg
     1141 gtttgttttt tccgcgaagt acgctagcaa ctactgcaga aagacgtcca agtggtacat
     1201 cagttgcgtc aactacgtac catttacgtt caacttggcc tggtttagcc ataaatgttg
     1261 ttttgttcat gatttctcct atatatagtt cgatttttgt ttacggtgat gggtgttcct
     1321 tcccatcgaa aagtatttgg aaggttccgg ggcctttcaa atggggtaaa caataccgcc
     1381 tactatcata ccaaatttca cccctaaaag tcaatagata tgaaaaatat ttttcggtaa
     1441 cttctcaaag taactgaaag ttatataatc aagttaattt gggaatttac ctattaactt
     1501 cggtagattt ttgggaattt ttttccttat ttttccctgt agttaaaatc cttttcaaaa
     1561 aatcaccttt tcttgatttt taatcctatt tgctgtatga taagggaaaa gaaaggggac
     1621 agagatatgg cttttaccaa tacccacatg cggtctgcta gttttggcat tgttaccagc
     1681 ttgcccgatg acatcattga ctctttttgg tatatcatcg atcatttctt aaaaaatgtc
     1741 tttgaattgg aagaagaact cgagtttcaa ttgcttaata accaaggaaa gattaccttc
     1801 cacttttcaa gtcaacacct ccctacagcc attgattttg actttaacca tcctttcgac
     1861 cctcgttatc ccccaagagt actggtttta gacatgtacg gtagagaaac tatcctcctc
     1921 ccagaagaaa atgacctatt ttaaaaactc tagccttcag ttgcaagtaa ctgaaaacta
     1981 gagtttttct attttttcaa agcatcatac aagttgcgga tcggttgttt taatgtcgga
     2041 tggataaaat gaggcgcaat ttcctgtaag gactcaagga caaaaaggcg ttccgctatg
     2101 taaggatgag gcaatatgag gtcgtctgta taaaggatct ggtcctccac aaagagcaag
     2161 tccaaatcaa tcaaacgagg tccccaatgc acttctctca cccgtcccag ctctgactca
     2221 atggctaaca aggtttctaa caagtcttgt gctggtagcc aggtttccac ctcaaccact
     2281 tgattggcaa agctatcctg ctccactcca ccccaaggct ccgtcgctaa gacactggac
     2341 tctttgagaa tatagatgcc acgagctcgc agtttgtcaa tggcttgctt caagtttgct
     2401 tgtttatctc ccatattgct tcctagggcg ataaaggctc gttgcttgcg gcgatgaatg
     2461 gttaccgagc aagtatctag tgacaaatgc accggtgccc aaggtttttt cagttccaac
     2521 ttcatttctt ggacaagagg ataagactca aaggtacgtt ccaccagttt ataggctacc
     2581 gtttcaatca aatcttcact cgtttcctga aaccaagtcg tccactgctg acataattct
     2641 ccgtaatgga cagaggctgt taaatccaag tctgtagctg ccttggtcat atcataggat
     2701 aggatggctg aaacgacaaa tttctgcccc aattctttct cactaggaaa aagaccatga
     2761 taggcaaaca tttccaaatc cttaatctgc agttgatcca taatgattcc tttctagaaa
     2821 aatccgcttg aagcggattc tttttatagc cccattaaac gataagcttg gtcgcggagg
     2881 tccttatctg tttcaaatag accacgagct actgtcgtca aggttgcagt gcctggtttt
     2941 ctaacaccac gcatactcat acacatatgt tccgcctcaa tgacaacaaa ggctccttta
     3001 gcacctagat agtccatcaa ggcatcggcc acttcgatat tcaaacgttc ttgaatttgt
     3061 ggttttttcg aataaacttc aaccgtacgg gctagcttag acaagcctgc cacacgacca
     3121 tctggaatgt aggcaatgtg cgctctacca taaaatggca agaagtggtg ttcacacatg
     3181 gtatggaaaa agatatcctt ttctaccacc atattatcgt caataatttc aaaggatttt
     3241 gacaaatgtt cctctgctgt ttgaccaaga cctgaaaaaa tctcttgata catacgggct
     3301 acacgagcag gtgtttcctg caagccctcg cgattagcgt tctctcctac agcctcgata
     3361 atcattttta cagctgcttc aatcttttgt gtatccattc tcgttcttcc tctccatcag
     3421 ataggccctc acttggctca agaaatacaa ggaacctgtg acaatcctaa ctgtttgttt
     3481 ctcttctttt ttatctgtca aattctgctc tagaaaatca tgccaatctt ggtagctgag
     3541 atttctagac ttagctgcct ctttcagcac gctttcatcc gtcgcccgac tatccgcaaa
     3601 atgtgttaga gtaagctcgg tatctggcat ggctcccagc aagtccaaca tatcctccaa
     3661 ggctttggtt ttgatacaag tgaagaggat ttccttatga taatccgcaa aacgttcttg
     3721 caaggttacc aacaaggcct tgatagcatg gggattatgg gctccatcca aaatcatcaa
     3781 gggatctctt gacacgattt ccaaacgccc tggccaactt gtttcttcca aggcttgacc
     3841 aagaaagtca ttgcttgcta gctctcgacc atcttcttga caaaaagtat caagtaaagc
     3901 tatggccatc ccagcattct ctatttggta caaaccaagc aggctagtct ggaagcgacc
     3961 ttgtctgaca gcacttgtat agtcaaagac ttcccctgtc accacacttt cttgatgacg
     4021 aacctgataa tctgtcccgt aggcaagtct cggcgcatct ttcccttccg caatgcggtc
     4081 aatcacagtc aaggcttctg gagcaatacg ccctgttacc aagggtatcc cttgtttgat
     4141 aatacctgcc ttctgctctg ctatggcctc caaggtgtca ccaagtaggg ccacatgatc
     4201 caagccaata gttgtaattc ctgtcaaaat gggctgacag acattggtac tatccaaaag
     4261 tccacccatg ccaacttcca tgatggccac atctacttgc tctgaggcaa agtagtcata
     4321 ggccagggct gtgataatct caaactcggt tgtgccctgt aaattggcga ccgcttctcc
     4381 ctccagcaaa gactgatagt ctgccatgag agcttctagc ctcgcttctg agatcgattc
     4441 cccattgatg ctaatctggt ctgtgtaatg aatgagatag ggcgagctaa acacgccaac
     4501 tctcagccct agcttttcta gcatcttttt caaaaaagca atagtcgagc ccttgccgtt
     4561 agtccctccg atatggagga ccttgagttt gaaatgggga ttgccacgca aagctaacag
     4621 ttccaccatt cgttccaatc caaaatgcgg ttgatccgtc cggtagttag caatccactg
     4681 attgttttca atttctttca tcttatttat attgttttaa atctaaattt tccgcttcat
     4741 cagccagacg aatggcagag gcaatttcaa ctgccatcct gtgactagct acgtcatgca
     4801 cgcgcaccac ttctacaccc tgtctcgcag cgatactagt tacatgagcc gaagccgtgt
     4861 cccgatttcg gaaaccaagc tctgtctcag gattgacttc aaacccactt tcttcaagga
     4921 tattgatgac aaatcgcttg cgcgacactc cgagaaagat tggatagccc ttctgatgta
     4981 gtttatccag gtcccgtaaa aggaggagat tttctttctt ggtcagacca aagccaattc
     5041 ctggatccaa caggatattt tctggtgtaa taccagcttc tgccgctctc gctagtgctc
     5101 gttcaaagaa agccaccatc aagtcttcga ttggcaatgt ttcaaagtca gctaactctt
     5161 tttctgtaaa ggtttgacca aagccaaaat gagggaagat aagcgaacta ggatgttgag
     5221 gtcgagccat gactggatta aacatgatga ctactttcgc tccagcttta gcaaccacat
     5281 gggccatttt ttcatcgccc atgagaccag tgatatcatt aactagattg gcaccagcag
     5341 ccaaagcagc ctctgccacc tgacttttcc aagtatcgat agaaataagg acatcacttt
     5401 ccttgcgaat cgctttgatc actggaacaa cacgctggat ttcctcttct atctcaacat
     5461 agctactgcc cggccgagtc gattctccgc cgatatcgag catactggct ccttctgcta
     5521 tcaatttacg agcctgctgg agtgcctgct caagagcaaa aaattgacca ccgtccgaaa
     5581 aggagtctgg ggttacattg ataattccgc aaataactgt ctttgcatga ttggctttac
     5641 ttgacatatc ggtcactccc tcaaggattt tcatcatatt atttttctat tttaccataa
     5701 aaagaaaaag atggacacga ttgcattcat ctttccctag tagaaagagg tcggttccta
     5761 cataaaaact tttacacaga aattcctaat gtccgactca taatcaccac aagagccaac
     5821 aaacagaaag caatcccatt aacaatcatg tgaagcaaga tcgacatttc caaacgttgg
     5881 gtcttgtagg ccgtccaaga tagaactgtc gacatacctc cataaatcaa taaagaaggt
     5941 aaattacttg gttgatgcaa taaagcaaac acaatcgtac cgactacaaa tcccaagttc
     6001 tccttgcctc ggaaaatctt tttaggaaca atcccacggc acaagatttc ctcacaaatt
     6061 ggagcaagca aggctagcaa gaagaagcta gaaatcagag aactattctg aaccatatcg
     6121 ttaatctgag actggttagc tgttgtcgtc tcatttgaca gttgcaacaa gatagaacca
     6181 agtatatttg acccgacaat aactaaataa ctcaagccca aacgtgccaa atctttagct
     6241 ctaaaaaaag aaaaattaaa actagctaac ttggttttac gagctcccat aataaataga
     6301 gccagaacca caattgaaag gccagtaact atcagccctg actgtagcag ggctacttct
     6361 tttaaagtca aaatagaggt aaccactagg ggaacctgat ataaaaggac ggcaagtaaa
     6421 aagaccaaaa tccagcctgc acgattcaac aattctttcc acatattttt ctctttcatc
     6481 acctatctcc tttgtattaa aaaagggggg aaatccccct gggtcattct attataaggc
     6541 catgctgatg tagttaagga taaacaaggc atccaaaatc caaatcatgg catgaacatc
     6601 tttaacttga cctttgacaa gcttagtcaa agtgtaagtc aagaaaccaa ctgcaatccc
     6661 ttgagtgata gagtagctga atcccataaa gatagatgtg aagaaggcag gaactgcttc
     6721 agacatatca tcccaatgga tatttttcaa gctaccaagc atcataatcc caacgataat
     6781 caagattgga gctgtagccg ctgttggtac gatcgctaga agtgggctaa agaagcttga
     6841 aatcgcaaaa cagatagcta caaccaaggc tgtcaaacca gtacgtccac ctgcaccgat
     6901 accagcagca gactcaacat aagtcgttac gtttgaagta cctgcaatgg caccgactgt
     6961 cgttccaatc aaatcagagt aaagagcctt atccaatttg gctgattggt gattttcacc
     7021 atttgtcgct acaataccaa ctttttcacc tgtaccgatc aaggtaccaa ttgtgtcaaa
     7081 aatatctgtc aatgagaagg caagaatggc catcagagtt tcaggcaagc gagctgtatc
     7141 tgaaaccaaa gctcccaaac cttctgaacc aagagctgca ccaaagattg tcttcaaatc
     7201 ttcaaaagct gcaccaacat ggttattagc aaaatcgata ctagacaaat caaccaaacc
     7261 aactgcaata gcaagaacag ttgttgtcaa gatagagaga ataattcccc ctttaatccc
     7321 tttgatgaca aagaagatag taatggcaag tcctgcaaga gccactaaaa cagctggatt
     7381 attaaagctg accaatcctg gaactgctga agagtttgct gcaatcgttg cttgagcttt
     7441 gtcagcccct tctcctacaa cagtatagtt gcctggatca atcgtgaatt tcaaaagtcc
     7501 agcattctta atccctacat aggcaaggaa gacaccgata ccagctgaaa tagctgagcg
     7561 aagagcattg ggaatcgatt caatgatcat tttacgaaca tttgtcaagg taataatcaa
     7621 tgaaataatc ccacagatga agaccatagc tagggcttct tgccaagaat aaccaagccc
     7681 gaatacaact gtaaaggtaa agaaggcatt gagtcccata cctggtgctt gggcataagg
     7741 taagttagca taaaaagcca tcatcaaggt acccgctact gcaccaataa tcgtcgctag
     7801 gaagacgccc tgagcaggca ttcctgtttg tgaaagtatt tgtgggttta caaagagaat
     7861 atagctcatt gcaaagaaag ttgttaaacc agcgagaacc tctgtacgaa cgtctgtacc
     7921 gttctctttt agtttaaata atttgtccat catcaatctc cttttaattt ttacgaacaa
     7981 taatagaatt atatcattta tcattcactt tttcaatgtc tttgtttgat ttgtttaaga
     8041 aattaatcca atttcatttt ttgtttggaa tctgcttttc tgcctgcttt ctgttataat
     8101 agaagacatc ttatctgaaa acacactaga aaggtccata tgacgaaaaa aattattgca
     8161 gttgacctgg atggaaccct gctcaactca gacagtcaaa tttctgactt taccaaaaga
     8221 accatcaaaa aagttgctga aaaaggccat caggttatta ttacgacagg tcgcccttac
     8281 cgtatgtcaa aagattttta ccgtgaactg ggcttagaca ctcctatgat taacttcaac
     8341 ggatccctta ctcatttacc agaccaagtt tgggattttg aaaagtgttt gactgtagac
     8401 aaaaaatatc tgctagatat ggttcaacgt tcagaggaca ttcaagccga ttttatcgct
     8461 ggagaatatc gtaaaaaatt ctacattaca aatcccaatg aagaaattgc caatcccaaa
     8521 ctatttggtg tagaagcttt ccagcctgaa gatcaattcc agcctgaatt ggtgaccaag
     8581 gaccctaact gtatcctctt gcagaccaga gccagtgaca aatatgcctt ggcaaaagaa
     8641 atgaacgcct tctaccagca tcaactttct atcaatacct ggggaggtcc gctcaatatc
     8701 cttgaatgta ccccaaaagg tgtcaacaag gcctttgctt tggactactt gctcaagata
     8761 atgaatcgtg acaaaaaaga tttgattgcc tttggagatg aacacaatga taccgaaatg
     8821 ctcgcttttg ctgggaaggg ttatgccatg aaaaatgcca atccagagct actcccttat
     8881 gcagatgagc aaatttccct taccaacgac caagatgggg ttgccaaaac cctacaagac
     8941 ttattcttat aacctatact gatactcaat gagaatcaaa gagcaaacta ggaagctagc
     9001 cgtaggttgc tcaaagcact gctttgaggt tgtagatgaa gctgacgtgg tttgaagaga
     9061 ttttcgaaga gtatgaccag cttgcaagct ggtctttgtg ctcttttcac agtctcatca
     9121 accagttaat cgattgttct actttttgtc tccaaaacct tggaaaaggc ttccttttgt
     9181 gatatacttc acatataaat acaagagagc tcgtcacact cgactctgtt gacaaaaaat
     9241 caatccagtc ccgttgtccc tgttctcggt caatatcaag gaggatagct atgaaagctg
     9301 ttgttgtaaa tccagaaagc actggtgttg ctattgaaga aaaagtactc cgtccacttg
     9361 aaactgggga agcacttgta gaagttgaat actgtggcgt ttgccacacc gacctccacg
     9421 ttgctcatgg tgactttggt caagtcccag gacgtgttct tgggcacgaa ggtatcggta
     9481 tcgttaaaga gattgctcca gatgtgaaaa gccttaaagt cggtgaccgc gtcagcgttg
     9541 cttggttctt tgaaggatgt ggcacttgcg aatactgtac aactggtcgc gaaacccttt
     9601 gccgtacagt gaaaaatgct ggctactcag tagacggtgg tatggctgaa caatgtatcg
     9661 taactgctga ctatgctgtc aaagttcctg acggacttga tccagcccaa gcttcttcta
     9721 tcacatgtgc tggagtaaca acctataaag ctatcaaaga agcaaaagtt gaaccaggcc
     9781 aatgggttgt tctttacggt gctggtggac ttggtaacct cgctgttcaa tacgctaaaa
     9841 aagtattcaa tgctcatgtt atcgcagtcg atatcaacaa tgacaaactt acccttgcaa
     9901 aagaagtagg cgctgacatt gtgattaacg gcctcgaagt tgaagatgta gctggactca
     9961 ttaaagaaaa aactgatgga ggagctcatt cagctgtcgt aactgctgtg tctaaagttg
    10021 ccttcaacca ggctgttgac tccattcgtg ctggtggtcg cgtcgtcgct gttggtcttc
    10081 cttctgaaat gatggaactc agcatcgtta aaacagtcct cgatggaatc caagtcatcg
    10141 gttctcttgt cggaactcgt aaagacttag aagaagcctt ccaatttggt gcagaaggtc
    10201 tggtagtccc agttgttcaa aaacgtccag tagaagatgc tgttgccatt ttcgacgaaa
    10261 tggaaaaagg ccaaatccaa ggacgtatgg tactcgactt cacccactaa tttaatagaa
    10321 acctatttta aaagttggaa gcttcttcca acttttttat atta
//
LOCUS       DBERCA010000059        10039 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.059, whole genome shotgun sequence.
ACCESSION   DBERCA010000059 DBERCA010000000
VERSION     DBERCA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.059"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..339
                     /locus_tag="ACL719_001689"
     CDS             <1..339
                     /locus_tag="ACL719_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0699621.1"
                     /translation="NCLSETTDKNWHKSDRIFVTNIGKPVHSSILSKSLQRANERLKK
                     PIPKHLPPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTSIYTQVTKNMKDEAIN
                     VLDKVMKKIF"
     gene            complement(477..1781)
                     /gene="eno"
                     /locus_tag="ACL719_001690"
     CDS             complement(477..1781)
                     /gene="eno"
                     /locus_tag="ACL719_001690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGS0699622.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            1945..2391
                     /locus_tag="ACL719_001691"
     CDS             1945..2391
                     /locus_tag="ACL719_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGS0699623.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2388..3503)
                     /locus_tag="ACL719_001692"
     CDS             complement(2388..3503)
                     /locus_tag="ACL719_001692"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGS0699624.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENRYKIPEGVQIHILADVVSPLCGYQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3512..3841)
                     /locus_tag="ACL719_001693"
     CDS             complement(3512..3841)
                     /locus_tag="ACL719_001693"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGS0699625.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3786..4157)
                     /locus_tag="ACL719_001694"
     CDS             complement(3786..4157)
                     /locus_tag="ACL719_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HGS0699626.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4216..5649)
                     /gene="glgA"
                     /locus_tag="ACL719_001695"
     CDS             complement(4216..5649)
                     /gene="glgA"
                     /locus_tag="ACL719_001695"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGS0699627.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5646..6785)
                     /gene="glgD"
                     /locus_tag="ACL719_001696"
     CDS             complement(5646..6785)
                     /gene="glgD"
                     /locus_tag="ACL719_001696"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGS0699628.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6775..7917)
                     /locus_tag="ACL719_001697"
     CDS             complement(6775..7917)
                     /locus_tag="ACL719_001697"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGS0699629.1"
                     /translation="MKNKMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7907..9835)
                     /gene="glgB"
                     /locus_tag="ACL719_001698"
     CDS             complement(7907..9835)
                     /gene="glgB"
                     /locus_tag="ACL719_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGS0699630.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFVERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3046 a         2219 c         1776 g         2998 t
ORIGIN      
        1 aattgtctaa gcgaaacaac cgataagaat tggcacaaaa gcgaccgtat ttttgttacc
       61 aatataggaa aaccagttca tagttctatc ttgagcaagt ctctccagcg agcaaacgaa
      121 cgccttaaaa aaccaattcc caaacatctg ccccctcaca tcttcagaca caccactatt
      181 agcatcttat cagaaaataa aattccttta aaaacaatca cggacagggt tggtcattcc
      241 gactctgaag tcactacttc catctacacc caggtcacaa agaatatgaa agatgaagca
      301 atcaatgtac tggataaagt tatgaaaaag attttttaaa aagttttgtc ccttttttgt
      361 cctctaaata caaaaatagc ccttcggata aaatccgagg ggctagaaac gttgttaaat
      421 caacggccga acttttgaat ttcatggttc gggataaaat agttcactga actattttat
      481 tttttaaggt tgtagaatga tttcaatcca cgatattcag ctacttcacc aagttggtct
      541 tcgatacgaa gcaattggtt gtatttagcg atgcggtctg tacgtgaaag tgaaccagtc
      601 ttgatttgtc ctgcgttagt tgcaactgca atatcagcga ttgttgaatc ttcagtttca
      661 cctgaacggt gtgatacaac agcagtgtaa ccagcttctt tagccatttc gatagcttca
      721 aaagtttcag taagagtacc gatttggtta actttgataa ggattgagtt agcagcacct
      781 tcttggatac cacgtgcaag gtagtcagtg tttgttacga agaagtcgtc accaacaagt
      841 tgtactttct taccaagacg ttcagtaaga gctttccaac catcccagtc gttttcatcc
      901 ataccatctt caatagtgat gattgggtat ttgttaacca attcttcaag gtagtcgatt
      961 tgttctgcag atgtacgaac agcagcacct tcaccttcaa atttagtgta gtcgtaaact
     1021 ttacgttctt tatcgtagaa ttctgatgaa gcacagtcaa atccgataaa tacgtcttta
     1081 cctggtacat atccagcagc ttcaatcgca gcaaggatag tttcaacacc atcttcagtt
     1141 ccttcgaaac gaggagcgaa tccaccttcg tcacctacgg cagtttccaa accacgtgat
     1201 ttaaggattt tcttaagagc gtggaagatt tcagcaccgt aacgaagggc ttctttaaat
     1261 gttggcgcac caactggcaa gatcatgaac tcttggaaag cgattggagc gtcagagtga
     1321 gaaccaccgt tgatgatgtt catcattgga gttggaagaa ctttagtgtt gaatccacca
     1381 agatagctgt aaagtgggat ttcaaggtag tcagcagcag cacgagctac agcgatagac
     1441 acaccgagga ttgcattcgc acccaattta cctttgttag gagtaccgtc aagtgcgatc
     1501 atagcacggt caatagcttg ttgatcacgt acatcgtagc caatgatagc ttcagcaatg
     1561 atgttgttta cgttgtcaac agctttttgt gtaccaagac caccgtaacg agatttgtca
     1621 ccgtcgcgaa gttcaactgc ttcgtgttca ccagtagaag ctcctgatgg aaccatacca
     1681 cgtccgaaag cacctgattc agtgtaaact tctacttcaa gtgttgggtt accgcgtgag
     1741 tctaggactt cgcgagcgta aacatcagta ataattgaca ttttttactc tccttatgag
     1801 ttaaattttt tacacctcta taatacctta aaacccctcc tttttcaaga aaaaacgtta
     1861 tctttgtgca acttttcctt aactttataa agtaatcgct ttcttttgtc tgttttattc
     1921 taacttttat gatatactgt tttcatgaca gatttatcaa aacaattact tgaaaaagct
     1981 catggtgggt taaaaataaa tccggatgag caaagacgct atcttggtac ttttgaggaa
     2041 agagttcttg gatatgtaga tattgacaca gcaaatagcc ctcagttaga aaaaggcttt
     2101 ttatttattt tagaaaacct tcaggaaaaa gcagagccac tatttgtgaa gatttcacca
     2161 actatcgaat ttgataagca agttttctac ttaaaagaag caaaagaaac tgatagtcaa
     2221 gccaccatag tatctgaaga gcatattact tctccttttg gcctggttat tcatagcaat
     2281 gcaccagttc aagtagaaga aaaagacctt cgacttgctt ttccaaaact ttgggaagtt
     2341 aaaaaggaag aaccagccaa aacatcctta tggaagaaat ggtttagcta aatcttgcac
     2401 atatttaata agtgcccaat attggcagcc gtgcgctcca gatagagact ggcatttttc
     2461 aaactatctt ctaaaggttc acttttctcc aaaatagaaa agacagcttg gatattttca
     2521 aatggtaggg gaggtaaatc ttcagcaaga ctaccgcaaa tagcaataac aggaactcca
     2581 acaggggttc tttttgcaac acctataggc gctttcccag caaagctttg actgtcaagt
     2641 cttccttctc caacaacaac caagtcagca tctgaaactt tcttatcaaa gttgattaag
     2701 tccaagcagg tatcaattcc agacacgata cttgcctgag caaaggcaca caaaccacca
     2761 gcaaggcctc cacctgctcc tgctccttta atttctaatg ttgcaggtga gaatttttca
     2821 taaaaatctt ggatcgcctg atctacgact gcaaacatag tcggatgtag acctttttga
     2881 ttgccaaaag tgtaagtcgc accttgataa ccacataagg gactcacgac atctgctaaa
     2941 atatgaattt gaacaccttc aggaatttta tagcgatttt ctgttgaaac agaagctaag
     3001 tttaataagg attgaccgga agcaggcaag acatttccat ccctatcata aaattgataa
     3061 cctaaaccag cagcaatccc cagtcctcca tcattactgg ccgtgccacc aacaccgata
     3121 taaatatctt taatcccttt agaaatgaga tggagaatca actctccaat accacaagtt
     3181 tggatttgaa gtggatttcg tttctctagc ggaatttttc caagaccaac caagtcagct
     3241 acttcaaata gtgccagttc ccctttttga aaatagcgca tggcttcttt ttgtccaaaa
     3301 gggtctgtca cttggatcca tttttctttt aggtcaagag aatgtcggat agcatctaca
     3361 gtaccttctc ccccatcacc aacagggcag aggagacatt ctacatctgc tatcgattgt
     3421 tggaagcctc tttttattgc ttcagctacc tgttgagctg tcaagctttc cttaaacgaa
     3481 tccggtgcaa ttacaatctt catattttcc ctcattctaa acagtcaatc aaaggaagaa
     3541 cttctaaaaa atccctcttg tcaacatgat gtggtatttc ttttttgagc acttctttgg
     3601 cacaaaaggc gattcctaac tccgccgact tcaacattaa tagattattg accccatcac
     3661 cgattgccac cgttctttct ttagaaagtt ttagtttctt tctccatttt tccagagttt
     3721 cttttttgac ctggggactt ataatttgtc caactaattt tcctgttaaa agaccttctt
     3781 tgacttcaag ctggttggca gtgaaatagg caataccaag ggattttgct aatctctcca
     3841 actattggtg taaatccacc agacaccaga ccaactagga tgccattctt ttggagaata
     3901 gagatgaact ctgggacatt tagcgataga tgaattgagt tgaagacgtt atcaaagacc
     3961 aaaataggaa gaccttccaa caaggacact ctttttctta aactgctttc aaagaccaac
     4021 tctcctcgca ttgctcgact tgtaatctgc gaaatttccg cctcatgacc tgcctctctc
     4081 cctaaaagat caatcacttc ttctaggatt aaggttccat ctacatccaa aacacacaag
     4141 ccttttactt gagacatcag ttctcctctc taaacagcct aaaaatcgta tgaagtcatc
     4201 atacgatttt atctattaat taactaaact atggtacaag tcaaggtatg acttgcaggc
     4261 tgtatcccat gagaagtcac tctccatagc ttgtttttgt aggtttctcc aaatgtctgg
     4321 atggtttcta tacaagtcca atgctgtttg gaaagtccaa tttaaccaat aaggagatag
     4381 attgtcaaag ctaaagccag taccgcttcc ttcgattgga ttgaaagcgc gaactgtatc
     4441 tcgcaagcct ccaacttcat ggaccaatgg caaggttcca taacgcatag ccatcatttg
     4501 agacaagcca cacggttcaa aacgacttgg catgaggaag aggtcacaag cagcgtagat
     4561 ttcctgagca agtttgacat caaaagtgat atttgttgat agcttgtctg ggtaaatctg
     4621 agcaaaccat gagaaagctc cttcaaaggc tggatcgcca gttcccaaaa gaacaatctg
     4681 aacatcttct tgcaagatat ggtgaagact ttcgaccacc acatcaaaac ctttttgacg
     4741 tgtcaaacga gaaacaattc ccaccagtgg aacgtctgct ctaacaggca agccaactct
     4801 ttcttgcaat tttgccttat ttttggcttt cccagacaaa tcttcctgat tgaaatgata
     4861 gtctaaaaga gcatccgtct gaggattata aagatcagca tcaatcccat tcacgatacc
     4921 agatacttta ccagactcca ttttaagaat ctgatccaaa ttacatccaa actgactagt
     4981 cataatttca tgagcatagc taggtgaaac ggttgaaaca cggttcgcat agagaatacc
     5041 tgccttcatc cagttcagac agttgttcca tcgaagggtg ccatcagcgt aacgttcaag
     5101 gccaactcca aacaaatcac ccaacattcc ttctgaaaat tgtccttgga attctaaatt
     5161 atgaatggtt aaaactgttt caatgtcctc ataggcttga atccaacggt atttttcctt
     5221 caacaagaaa ggaatcatag ctgtatggta gtcatgaaca tggagaagat caggaataaa
     5281 gtcaatcctt tccatagcct caatggcagc cagttggaaa aaggcaaagc gttctccgtc
     5341 atcaaaatca ccgtaaacat gaccacggaa gaaataatat tgattgtcaa taaagtagaa
     5401 ggttacacca tttaatactg ttttcttaat tccacaatac tgtctgcgcc aaccaacgct
     5461 cacctcaaaa tgaagcacat cttcaatctg atttccaaat ttagcctcta ccatatcata
     5521 gtagggtaaa atcactgcaa cttcgtgccc agcttttacc agtgattttg gaagagcgcc
     5581 aatgacgtct cccaaaccac ctgtttttga aaaaggtgca ccctctgctg ctacaaataa
     5641 aattttcatg aatgaatatc ctctgttact ttagcacctt tcttaaccac aactggatgt
     5701 tctgcagttc ctcgaatcac aacaccaggc tcaacttcaa cccctttgtc caagatagca
     5761 tattcgacct gagccccttt tccaataaca acacgaggga agagcaggct atctttaacc
     5821 aagctatcct tatggacatg aatattacgt gatagaacag aattagctac ttgaccttca
     5881 ataatactac cagaggcaaa ctgagaagtg cttaccttag atgtattagc atagtaagtt
     5941 ggctcttcgt ttttgacctt tgtataaatc ttttggtttg gtgagaaaag agaatagaat
     6001 ttttgtgatt caagcatatc gatattcgct tgataataag atttaacaga gtgaatattg
     6061 gctagatagc ccgtgtactc gtaggcgaaa gctccctctt ttacagccaa atcccgtaaa
     6121 acatagcgca atttctttgg atgttctttt ttagcttctt cttccaagtg ttcaatcaac
     6181 caaggtgtat caacgacaaa gatatctgta gacatattga acatttcagc tgttgacttg
     6241 ctatcaaaga gtttatgaga aagaacatgg tctgtttcat ctacatccaa gattgcattt
     6301 acttctgaaa tatctttctt agctagtttt ttataaacta cagtgatagg ctcttttgtt
     6361 gtactatgta ggtggaaaac ttggttcaaa tcaatgttaa taagaacatc gcagttgagg
     6421 gcaaccgttt ggtttgagcc agaacgtttc aaataagtaa gaagctgttg gtagtattct
     6481 tttccaactg tactactttc tacacgggta ttgtaaattc ctagatagta atgactaaga
     6541 agggttgata agccccactc gcgtcctgaa cgaatatggt caaatactga gctgatatta
     6601 tcctgctgga aaataccaaa gacactacga acacctgcat tagcaaggct tgaaagtggg
     6661 aagtcaatca aacgatattt cccaccaaat ggcaaacttg ctactggacg gtggtccgtc
     6721 aatgtcgaca tattgtgaaa accaactgta tttcctaaaa tggcagaata tttatcaatc
     6781 ttcatctgtt gctaccccca ctacttcatt atatcctaca acttgtactt catctgttcc
     6841 atcaatttcg acaccgtcag aaataatcgc accttcacca ataatggcac gtttaatctt
     6901 agctccttga ccaatgatag ctccactcat gataactgaa tcaaggactt ccgctccttc
     6961 gcgaacttgc gcgcctgttg aaaggataga atgtttaaca gctccatcaa cgaaacatcc
     7021 gtctacaact aatgagtctt ccacatgagc atttgccccg aggaagtttg gtggtgaaat
     7081 caagtttctt gagtaaatct tccattgacg gttacgacta tccaaggcat tttctggaga
     7141 aatatactcc atgttcgctt cccaaagtga ctcaatagta ccaacatctt tccaataacc
     7201 actaaattcg taagcataaa cactttcacc tgactcaagg taatttggaa tgacattttt
     7261 accaaagtct gacatgccaa ccttgctctt ttcagcagcg actaacatat tacgaaggcg
     7321 ttgccaatca aaaatgtaga ttcccataga agcttttgta gatttaggtt gagctggttt
     7381 ttcttcaaat tcaacaatac gattgttagc atctgtgttc atgataccaa aacggcttgc
     7441 ttctttaaga gggacgtcta aaactgctac tgtcaagctg gcattattat ccttatgaga
     7501 ctggagcata tcatcatagt ccattttgta gatgtggtcc ccagacaaaa tcaagacata
     7561 ctcaggattg acactgtcga tatagtcgat attttggtaa atagcgtgac tagttccctc
     7621 aaaccaacga tttccttcac ttgcagaata aggttgaaga atagagacgc ctgaattaat
     7681 accgtctagt ccccagcttg aaccattccc aatatggttg ttgagagcaa gtggttgata
     7741 ctgtgtaacg accccaacat tatgaatccc tgagttggca cagtttgata gggcaaagtc
     7801 aatgatacgg tagcgcccac caaattgcac agctggtttt gcgatgcttt gagtgagttt
     7861 accgagacga gttccttgcc caccagcaag aatcaaagct aacattttat ttttcatttt
     7921 ctactccttt ttggttttta tttgtgacgg ttttagtaga tttcaagcga cgtttgattt
     7981 tccatacact tgctcccata gccggtagag taaaggttaa ggtctgctca taatctttcc
     8041 ataggccttc ttgcgtttga acagtttgat tatgttcttt ccaaacgcct ccccactctt
     8101 ccaactcagt attccatact tcttcgtaaa ttcctgcaac gggtagtccg attgtaaaat
     8161 ctttccgctc aacaggtacc atgttaaaga tacagactaa catttctccc tttttaccct
     8221 tacgaataaa ggaaagaaca ctctggtctc gattatccgc atcaatgatt tcaataccat
     8281 catagctggt atcgatttcc cacagacagc gatgatcttt gtaaaactgg tttagctgag
     8341 aagtgaaata cttcatctta gcattcattg ggtcttctag gttagaccat tccaactgtt
     8401 cttcagattt ccattctagg aattgaccgt attcgctacc catgaagagc aatttcttac
     8461 cagggtgaca aatttggtac gtatagagat tgcgcaagcc tgcgaattga ttgtaacgat
     8521 ctccccacat cttatgcatc atactcttct tgccatgaac cacttcatcg tgcgagaatg
     8581 gcaagagata attctccttg aaaacataca taaagctgaa agtcaccagg ttaaagtcat
     8641 atttacgata aatcggatct tcttcgtaga aacggaggat atcattcatc cagcccatgt
     8701 tccatttgta gtcaaatcct agaccaccaa tctctttcat tcccgtaatc ttggtcgcag
     8761 acgaactttc ttctgcaatc atcatcacat ctggatattc taacttaata acttcattca
     8821 agcgttgaag aaaataataa ccttcatagt taagatttcc gccatcttta ttaggtgtcc
     8881 atggagcatt atcatagtcc aaatagagca tgttgctaac agcatccaca cgaataccat
     8941 ccaaatgata gacatcaatc caatgcttaa tgcaagaaat taagaaggac tggacttcat
     9001 tttttccaag gtcaaaatta agggcacccc aaccatggtt atgagcctta ttatggtctt
     9061 ggtattcaaa agtcggtgtc ccatcataat aggctaaggc atcatcgttg atggtaaagt
     9121 gacctggtac ccagtccaca ataaccccaa tattatgggt atgacactcc tcgacaaaat
     9181 cttgaaactc ctctggtctg ccataagcat gctctaaagc gaagtaaccc ataagctgat
     9241 acccccaact caagcccaaa ggatgggaca tcaagggcat aaactcaata tgagtatagt
     9301 tcatttcaac gagataagga atgagttcat ccttgagctg ggcaaaacta taaggactgc
     9361 catcaggatt tctcttccaa gagccagcat gaacctcgta aatattgaca ggacgttcaa
     9421 caaagcccca acgttttctt cgtgccagcc aaagtccatc cctccatttc ttatcaggaa
     9481 gctctgttac gattgcccct gttcctggac gagcctcata cctgacagca aaagggtcaa
     9541 tcttcatcag ttgatgacca ttttgacgtg tgacatgata tttgtaaata tgcccttctt
     9601 gagccatatt ggtaaagact tcccagaccc caaagtcatt tcttaccatt ggaatctgat
     9661 tttcaatcca gttggtaaaa tcaccaacca agtgaacagc ctgagcatta ggtgcccaaa
     9721 cacggaaggt atagccatgc tctccattta gttcttccct atgtgctcct agataatgtt
     9781 ggagataaaa attttcaccc gtcataaagg tttttaatgc ttctctatta tccatatact
     9841 ccccttctcc tgtaagcgtt ttctatgttt ttattatact acctttttag agaagattca
     9901 agtaaattac tatacttctt taattatttt gaaaatctac aacaagttca cttactcgtt
     9961 caattgtaaa tcaatatttt ttcaaaaaat tgcgaaaacg cctttctttt tctactatag
    10021 tgaaatgaaa taaaacatg
//
LOCUS       DBERCA010000060         9777 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.060, whole genome shotgun sequence.
ACCESSION   DBERCA010000060 DBERCA010000000
VERSION     DBERCA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.060"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            88..1026
                     /gene="msrB"
                     /locus_tag="ACL719_001699"
     CDS             88..1026
                     /gene="msrB"
                     /locus_tag="ACL719_001699"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS0699631.1"
                     /translation="MTEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            1084..2808
                     /locus_tag="ACL719_001700"
     CDS             1084..2808
                     /locus_tag="ACL719_001700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699632.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLIPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            2810..4558
                     /locus_tag="ACL719_001701"
     CDS             2810..4558
                     /locus_tag="ACL719_001701"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699633.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFVKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            4860..6119
                     /locus_tag="ACL719_001702"
     CDS             4860..6119
                     /locus_tag="ACL719_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGS0699634.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    6195..6330
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6369..6869
                     /gene="rplJ"
                     /locus_tag="ACL719_001703"
     CDS             6369..6869
                     /gene="rplJ"
                     /locus_tag="ACL719_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGS0699635.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            6945..7313
                     /gene="rplL"
                     /locus_tag="ACL719_001704"
     CDS             6945..7313
                     /gene="rplL"
                     /locus_tag="ACL719_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGS0699636.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            7500..7670
                     /locus_tag="ACL719_001705"
     CDS             7500..7670
                     /locus_tag="ACL719_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699637.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            7670..7918
                     /locus_tag="ACL719_001706"
                     /pseudo
     CDS             7670..7918
                     /locus_tag="ACL719_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            7932..8272
                     /locus_tag="ACL719_001707"
                     /pseudo
     CDS             7932..8272
                     /locus_tag="ACL719_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8265..8645
                     /locus_tag="ACL719_001708"
     CDS             8265..8645
                     /locus_tag="ACL719_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS0699638.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            8658..8891
                     /locus_tag="ACL719_001709"
                     /pseudo
     CDS             8658..8891
                     /locus_tag="ACL719_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8894..9481
                     /locus_tag="ACL719_001710"
     CDS             8894..9481
                     /locus_tag="ACL719_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0699639.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            9683..>9777
                     /locus_tag="ACL719_001711"
     CDS             9683..>9777
                     /locus_tag="ACL719_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS0699640.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFST"
BASE COUNT         2910 a         1831 c         2109 g         2927 t
ORIGIN      
        1 aacatatata cggtaaggtg acgctgacgt ggtttgaaga gattttcgaa gagtattagt
       61 taaaaacgtg ataaaggaga aataaaaatg acagaaattt atctagcagg tggttgtttt
      121 tggggcctag aggaatattt ttcacgcatt tctggagtgc tagaaaccag tgttggctac
      181 gctaatggtc aagtcgaaac gaccaattac cagttgctca aggaaacaga ccatgcagaa
      241 acggtccaag tgatttacga tgagaaggaa gtgtcactca gagagatttt actttattat
      301 ttccgagtta tcgatcctct atctatcaat caacaaggga atgaccgtgg tcgccaatat
      361 cgaactggga tttattatca ggatgaagca gatttgccag ctatctacac agtggtgcag
      421 gagcaggaac gcatgctggg tcgaaagatt gcagtagaag tggagcaatt acgccactac
      481 attctggctg aagactacca ccaagactat ctcaggaaga atccttcagg ttactgtcat
      541 atcgatgtga ccgatgctga taagccattg attgatgcag caaactatga aaagcctagt
      601 caagaggtgt tgaaggccag tctatctgaa gagtcttatc gtgtcacaca agaagctgct
      661 acagaggctc catttaccaa tgcctatgac caaacctttg aagaggggat ttatgtagat
      721 attacgacag gtgagccact cttttttgcc aaggataagt ttgcttcagg ttgtggttgg
      781 ccaagtttta gccgtccgat ttccaaagag ttgattcatt attacaagga tctgagccat
      841 ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg ctcacttggg tcatgttttc
      901 acagatggac cgcgggagtt aggcggcctc cgttactgta tcaattctgc ttctttacgc
      961 tttgtggcca aggatgagat ggaaaaagca ggatatggct atctattgcc ttacttaaac
     1021 aaataaaaca gagagtgggg cttcccactt tcttcatttc tagaatatga atagaaggga
     1081 tttatgaaac acctattatc ttacttcaaa ccctacatca aggaatcaat tttagccccc
     1141 ttgttcaagc tgttagaagc tgtttttgag ctcttggttc ccatggtgat tgctgggatt
     1201 gttgaccaat ctttacctca gggagatcaa ggtcatctct ggatgcagat tggcctgctc
     1261 cttatctttg cagtaattgg cgttttagtg gccttgatag ctcaatttta ctcagcaaag
     1321 gcagcagtag gttttgctaa ggaattgaca aacgatcttt atcgtcatat tctttccttg
     1381 cccaaggaca gcagagaccg tctgacaact tctagtttgg ttactcgctt gacttcggat
     1441 acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc tctttttacg agcgcccatt
     1501 atcgtttttg gtgccatttt tatggcttat cgaatctcag ctgagttgac tttctggttc
     1561 ttagtcatgg ttgccatttt gaccattgtc attgtagggt tatctcgatt ggtcaatcct
     1621 ctctacagta gtctcagaaa gaaaacggac caactggttc aggaaacgcg ccagcaattg
     1681 caagggatgc gggttattcg tgcttttggt caagaaaaac gagagttaca gatttttcaa
     1741 acccttaacc aagtttatgc tagattacaa gaaaagacag gtttcttgtc tagtttatta
     1801 atacctctga cctatctgat tgtcaatgga actcttctcg ttattatctg gcaaggctat
     1861 atttcaattc aaggaggagt actcagtcaa ggtgctctca ttgctcttat caattacctc
     1921 ttacagattt tggtggaatt ggtcaagcta gccatgctga tcaattccct caaccagtcc
     1981 tatatctcag ccaagagaat cgaggaggtc tttgcggaat ctcccgaaaa catccattca
     2041 gaattagaac aaaagcaagt taccagtggt cgggttttac aagtccaaga attgactttt
     2101 acctatcctg atgcggccca gccttctctg agagacattt cctttgatat gactcaagga
     2161 caaatccttg gtatcattgg ggggactggt tctggtaaat caagcttggt gcaactctta
     2221 cttggacttt atccagtaga caaggggaac attgaccttt atcaaaatgg acgtagtcct
     2281 cttaatttgg agcagtggcg gtcttggatt gcctatgtac ctcaaaaggt caaactcttt
     2341 aagggaacta ttcgttccaa cttgacttta ggtttaaatc aagaagtatc tgaccagaaa
     2401 ctctggcagg ccttggagat tgcgcaagct aaggattttg tcagtgaaaa ggaaggactt
     2461 ttggatgccc tagttgaggc aggggggcga aatttctcag gtggacaaaa acaaaggttg
     2521 tctatcgccc gagcagtctt gcgccaagct ccgtttatca tcctagatga tgcaacctcg
     2581 gcactggata ccattacaga gtccaagctc ttgaaagcta ttagagaaaa ttttccaaac
     2641 acgagcttaa ttttgatctc tcaacgaacc tcgactttac aaatggcgga ccagattctc
     2701 ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg atgacttgat gaaatccagc
     2761 caagtctatc gtgaaatcaa tgcatcccaa catggaaagg aggactagaa tgaaacgaca
     2821 aactgtaaac cagacgctca aacgtttagc cgtagattta gcaaaccatc ccttcctcct
     2881 tttcctagcc tttctaggaa ctattgccca agttggctta tcaatttacc tacctattct
     2941 gattgggcag gtcattgacc aagtcctagt ggctggttca tcaccagttt tttggcagat
     3001 ttttctccag atgctcttgg tggtaatagg aaatactctg gtacaatggg ccaatcctct
     3061 cctctataat cgtctaatct tctcttatac cagagattta cgggagcgaa tcatccataa
     3121 actccatcgg ttaccgattg cctttgtaga taggcaaggt agtggagaga tggttagtcg
     3181 tgtaaccacg gacatcgaac agttggcagc tggcttgacc atgattttta accaattttt
     3241 cattggtgtt ttgatgattt tggttagtat tctagccatg ctccaaattc atctcctcat
     3301 gactctctta gtcttgctgt tgacgccact gtccatggtg atttcacgct ttattgccaa
     3361 gagatcctat catctcttcc agaagcaaac agagacgagg ggaattcaga ctcagttgat
     3421 tgaagaatcg cttagccagc agactataat ccagtccttc aatgctcaaa cagaatttat
     3481 ccaaagattg cgtgaggctc atgacaacta ctcaggctat tctcagtcag ccatctttta
     3541 ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca ctcatttatg cccttttagc
     3601 tggagtagga gcttatcgta tcatgatggg ttcaaccttg accgtcggtc gtttagtgac
     3661 ttttttgaac tatgttcagc aatacaccaa gccctttaat gatatttctt cagtgctagc
     3721 tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat ggagtcttag atagccctga
     3781 agtggctgaa acaggtaagg aagtcttgac cagtgaccaa gttaagggag ctatttcctt
     3841 taaacatgtc tcttttggct accatcctga aaaaattttg attaaggact tgtctatcga
     3901 tattccagct ggtagtaagg tagccatcgt tggtccgaca ggtgctggaa aatcaactct
     3961 tatcaatctc cttatgcgtt tttatcccat tagctcggga gatatcttgc tggatgggca
     4021 atccatttat gattatacac gagcatcatt gagacagcag tttggcatgg tgcttcaaga
     4081 aacctggctc acacaaggga ccattcatga taatattgcc tttggcaatc ctgaagccag
     4141 tcgagagcaa gtaattgctg ctgccaaagc agctaatgca gactttttca tccaacagtt
     4201 gccacaggga tacgatacca agttggaaaa tgctggagaa tctctctctg tcggccaagc
     4261 tcagctcttg accatagccc gagtctttct ggctattcca aagattctta tcttagacga
     4321 ggcaacttct tccattgata cacggacaga agtgctggta caggatgcct ttgtaaaact
     4381 catgaagggc cgcacaagtt tcatcattgc tcaccgtttg tcaaccattc aggatgcgga
     4441 tttaattctt gtcttagtag atggtgatat tgttgaatat ggtaaccatc aagaactcat
     4501 ggatagaaag ggtaagtatt accaaatgca aaaagctgcg gcttttagtt ctgaataagc
     4561 cattctcttt tgaaagttta tggacgaaaa aagttgcctt cgagtgactt ttttgttaca
     4621 atagctagaa aaattgttca ctgtaatact caatgaaaat caaagagcaa actaggaagc
     4681 tagccgtagg ttgctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagct
     4741 caaaacactg ttttgaggtt acagatagaa ctgacgaagt cagtaaccac acctacggca
     4801 aagtgaatct gaagtggttt gaagagagta caacttgtct tttagaaaag gagcctataa
     4861 tgaaagtctt tcagcatgta aatatcgtga cttgtgatca agatttccat gtttatcttg
     4921 atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat aagccagcgt
     4981 ttttagagca agctgagcag attatagact atcagggagc ttggattatg cctggtttgg
     5041 tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat gacagcaatc
     5101 tccatgaatg gctcaatgac tatatttggc cagcagaatc tgagtttact cccgacatga
     5161 ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca acaaccttta
     5221 acgatatgta taatcccaat ggtgtggata tccagcaaat ttatcaggtg gtgaaaactt
     5281 ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa acaactgctg
     5341 agactataag tagaactcga tccatcatag acgaaatctt aaaatataaa aatccaaatt
     5401 tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg ctggaagcga
     5461 gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg gagaccaagg
     5521 aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt cttgaagaac
     5581 tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac gagagagaaa
     5641 ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt aacctcaaac
     5701 tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca gtcggaattg
     5761 cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga aggactgcag
     5821 ctcttcttca gaagatgaaa agtggggatg ccagccagtt tccaatcgaa acagctctca
     5881 aggtactgac aatcgaaggg gctaaggtcc ttggaatgga aaatcagata ggaagtctgg
     5941 aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt catctccaac
     6001 ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat gtagatgatg
     6061 tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta gaactttaaa
     6121 agaaaaatta cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct ttttttgact
     6181 atgctatact tatatacggt ttaaaaaaac tgcctaagac agtaggggag ctcgactcat
     6241 aaatatccta ccgaggacaa aacgtatcat gtaaaaagaa gcgtattgta ctttcgtgtc
     6301 taggtttggg cgcgtttttc tttttgaaaa attccccaag caaaataatt acggaggtga
     6361 acacactaat gagtgaagca attattgcta aaaaagcgga actagttgac gtagtagctg
     6421 aaaaaatgaa agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg acagttgagc
     6481 aagatacagt tcttcgtcgt gagcttcgtg gaagcgaagt tgagtataaa gttattaaaa
     6541 actcaatctt gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca tctgtatttg
     6601 ttggaccatc tgcagtagca ttttctaatg aagatgttat cgcaccagcg aaaatcttga
     6661 acgacttttc taaaaacgct gaagcacttg aaattaaagg tggtgcaatc gaaggcgctg
     6721 tcgcatctaa agaagagatt cttgcacttg caactcttcc aaaccgcgaa ggacttcttt
     6781 ctatgctcct ttctgtactt caagcgccag tgcgcaacgt tgctcttgca gtcaaagcgg
     6841 ttgcagaaag caaagaagac gcggcttaat cttaagctac acagcgtagc ctagctacga
     6901 aaaaaactat tataaaattt aaaacttatt tggaggaaat aacaatggca ttgaacattg
     6961 aaaacattat tgctgaaatt aaagaagctt caatccttga attgaacgac cttgtaaaag
     7021 ctatcgaaga agaatttggt gtaactgcag ctgctcctgt agctgttgct gcagctgatg
     7081 cagctgatgc tggtgctgct aaagattcat tcgacgttga attgacatct gcaggcgaca
     7141 aaaaagttgg cgttatcaaa gttgtacgtg aaatcactgg tcttggtctt aaagaagcta
     7201 aagaacttgt tgacggtgca ccagcacttg ttaaagaagg cgttgcaact gcagaagctg
     7261 aagaaatcaa agctaaattg gaagaagctg gagcttcagt tactcttaaa taatagagta
     7321 tcgcaatttg attgcggaat ccctatttac cagtctttga gagcgataag cgattgatag
     7381 aaactgataa attgatttgg tttcctgcca aaaattcttt ggcaggattt ttgttttaat
     7441 aaactttcgg gggtagacac tagattgtct accccttttt ttgtgtttcg gaggtaatta
     7501 tgcaaaagga aggacagtca tcacgtcaat tcgtgaagag tcatctcatg actatcatgg
     7561 gagttgatat tgtaaaagca actcagttag ttgatgaaat ggaacaagta ggtttaatcc
     7621 gatttgatga atttggaaat gttggaattt tagtcttgga gggacaataa tgaagcgtat
     7681 taccgctaat caatatcaga catcagagcg ttattataag ctccccaaac ttttatttga
     7741 gagtgaacgg tataagaata tgaaactgga ggttaaggtg gtttactcag ttttaaaaga
     7801 tcggttggag ttgtctttga gcaaaggttg gattgatgag gatggggcta tttatttgat
     7861 ttattccaat tcaaatttga tggcactttt aggctgttca aagtcaaaat tactctccat
     7921 gtgagtttga agtgacattt ttagatgatt accataaaaa acataactac ccactatttt
     7981 acgaatccta tcttcaaaac gttatggaat tccttgaaag tcaagacata aagaatgggg
     8041 ttgatgcctt tgtagatgat catcaaaatc tcgtttttgt tttatatgga caaggctatc
     8101 gagccgaggg aaaagaggga atacttacaa cccaagtaac tgtaaaagct tatgatgaag
     8161 acaagaaacc gattaacttc gcaaatttat tagattcctt aatttattag attccttaat
     8221 cgtctcagaa tatcaaatgg aaccgaatct ttgggaggtc tcccatgatt gatctctatc
     8281 taagtaaaaa tagacaaaga aatcaacttc ttttagattt cttccaaaac tatggcatag
     8341 aggtatcttg tcattcagtt tctgaaatga caaaggacaa attaattgag atgatgagct
     8401 attcttcaga ttgttttgag tttttatctc ccaatttatt acgatttaag aaccgtgaca
     8461 atctaagatt aacggatttc gttgagataa ttttaaaaaa tcctgaacta accatcagac
     8521 ttcctcttgc ggtttcaaat aagcgagttt atccaagtct gaatttggaa gaggctagag
     8581 ctctattgcc aagagatacg aaacaattga tttacatggc gcaaacacat tatttatcta
     8641 actaaaggag aagcaatatg gctgaacgat tttgggagaa cttgtccatt attttggctg
     8701 aaagaaatat cagctggatt gagttaacca gaaaaatgtt tgcgggagag tttcactatc
     8761 caagtgaatt gaatcgtttg tatcaaaaga ttcgccacta gaagatggaa caatgaatgc
     8821 ctcaaagtcc atgggtagaa aggattgtgc aggtattaga tttagattat gaagatttat
     8881 ttcggaggta actatgaaaa gaataattcc agtttatata ttccaacaag taaatgtcct
     8941 attggtatct ctatacttat tgaaatttct ttgtatcggt gagttaacta tactacagat
     9001 tctctatggt tcgtcactca tttctttttt atggatgtat ggccaacgca aacaagcgca
     9061 taaggtcaat atgaaatcta ggatgaaatg gcttggtgtt gaatttgtta gcctactgat
     9121 tataagtcta tgttttagtc tgattcatgc tcaaggaagc acgaatcaag caaacttaat
     9181 tggccttcaa catcaaattc cttggttttc atttttattg ttcttaatca atgcgagtat
     9241 ggttgaagaa tttctgtatc gagaaatttt atggaatttg gtcagaaaat tagatatcag
     9301 agttgctttg acaagtgttt tatttgcctt agcacatcat acaggaacca ttctagcttg
     9361 gtgtttgtat gtttcacttg ggatgttttt aggtatggtg cgctataaat ctgacttatg
     9421 gggcagtatg ggactacatt tggtgtggaa cctactagtt tatagtttgc tgctttttta
     9481 aaataggtga cctgattttt aggtaacttc taaaactaac ttccagtttt gggagagaga
     9541 tggaagttac tttgaaaagt tacggaaaca ttattttcta tgtgacaaca ttgtataatt
     9601 gcttgcattt tactggtgga gagagtataa tgagggtgga taaagtactt aatcccaaaa
     9661 atttaaatag gaggagctag ctatggcaga agaagtatta aatcttcaat tagtatctgt
     9721 tcaagttgac gaaacagatg aagttgatgg aatgcgattt agtacattca gtacaaa
//
LOCUS       DBERCA010000061         9611 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.061, whole genome shotgun sequence.
ACCESSION   DBERCA010000061 DBERCA010000000
VERSION     DBERCA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.061"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..605
                     /locus_tag="ACL719_001712"
     CDS             <1..605
                     /locus_tag="ACL719_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0699641.1"
                     /translation="NEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLENVS
                     GLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSEIR
                     EIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQFE
                     KAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            636..839
                     /locus_tag="ACL719_001713"
     CDS             636..839
                     /locus_tag="ACL719_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS0699642.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(932..1171)
                     /locus_tag="ACL719_001714"
     CDS             complement(932..1171)
                     /locus_tag="ACL719_001714"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0699643.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            1450..1695
                     /locus_tag="ACL719_001715"
     CDS             1450..1695
                     /locus_tag="ACL719_001715"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS0699644.1"
                     /translation="MAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDISNHHDSESFF
                     ANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(1973..2743)
                     /locus_tag="ACL719_001716"
     CDS             complement(1973..2743)
                     /locus_tag="ACL719_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGS0699645.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLYQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(2758..3606)
                     /locus_tag="ACL719_001717"
     CDS             complement(2758..3606)
                     /locus_tag="ACL719_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGS0699646.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(3603..5078)
                     /locus_tag="ACL719_001718"
     CDS             complement(3603..5078)
                     /locus_tag="ACL719_001718"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGS0699647.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHTLGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(5402..6758)
                     /locus_tag="ACL719_001719"
                     /pseudo
     CDS             complement(5402..6758)
                     /locus_tag="ACL719_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(6768..7085)
                     /locus_tag="ACL719_001720"
     CDS             complement(6768..7085)
                     /locus_tag="ACL719_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0699648.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(7102..7419)
                     /locus_tag="ACL719_001721"
     CDS             complement(7102..7419)
                     /locus_tag="ACL719_001721"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699649.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(7434..8330)
                     /locus_tag="ACL719_001722"
     CDS             complement(7434..8330)
                     /locus_tag="ACL719_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGS0699650.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKTVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(8333..9595)
                     /locus_tag="ACL719_001723"
     CDS             complement(8333..9595)
                     /locus_tag="ACL719_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0699651.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPSWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3322 a         2021 c         1443 g         2825 t
ORIGIN      
        1 caaacgaaaa aaacacaaac actaacgtag aaaagaaaga tgctactgtt gtagctcacg
       61 aaatcaaagg ggaacttact tacgaagata aagttatcca aaaaatcatt ggtctttcac
      121 tagaaaacgt ttcaggtctt ttgggaatcg atggtggttt cttctcaaat cttaaagaaa
      181 aaatcgttaa cagcgatgac gtaacaagtg gtgttaacgt agaagttggt aaaacacaag
      241 ttgcagttga cttaaacgtt attgttgagt accaaaaaaa tgttccagct ttatattcag
      301 aaatcagaga aatcgtatct tcagaagttg ctaaaatgac tgacttggaa attgttgaaa
      361 tcaacgtaaa cgttgtcgac atcaaaacta aagaacagca tgaagcagac tcagtaagcc
      421 ttcaagatcg cgtatctgac gttgctgaat caacaggaga attcacttca gaacaattcg
      481 aaaaagctaa atctggtctt ggatctggtt tctcaactgt tcaagaaaaa gttagcgaag
      541 gtgtagaagc tgttaaaggt gcagcaaatg gtgtagtatc tcacgaaaac actcgtgtaa
      601 actaagataa aataaatata acaggagaaa ttatcatgtc agtagaagaa aaattaaatc
      661 aagctaaagg ttctattaaa gaaggtgttg ggaaagccat cggtgatgaa aaaatggaaa
      721 aagaaggagc agctgaaaaa gttgtttcta aagtaaaaga agttgccgaa gacgctaaag
      781 acgctgtaga aggtgctgta gaaggtgtta aaaacatgtt gagtggcgac gataaataag
      841 gttattggta acattagtca aaagagtctg agtcaagatg attctcagaa aacaaaaagc
      901 tagagattcc caattgcgga actctagctt tttaattttg cctctttctc ttattatatt
      961 tcagcaggtt gttggccatg agtatgaatc ccatgtcaat tctcacttga cgcttacctc
     1021 tcagatgaca tctcttataa cccaaacaaa cctttatctg cccaaagaca gatttcatat
     1081 caatcttacg tttagcgaaa atttgtctac ccttggaaga taaaagtgcc tgatattctt
     1141 tagtttttaa acactggtaa cgttcattca tatacagtcc cttttgaggg gctgattcag
     1201 gttcataatc gcagtcaaca ttgatttcaa ggctgtttgc tttctatctc ccctttttca
     1261 tgatatattt tcgtacaggt tcttcaatag tttctatagt aaaatgaacc aaaaatagta
     1321 cacgttgcgg tataatgttc ttgtggcata ttcattagat tttcgtaaac agttctctcc
     1381 tatttgtgag cgaacaagta tagtcaattg attgtcagtt tgaagaccaa gagtcgtaag
     1441 gcaaaggata tggcagaatc catccaaggg tggctggcac aatttctagt gaacctcttt
     1501 aaatctatta cgtttgactg tggaaaagag ttttcaaaat ggaaagatat ttccaatcat
     1561 cacgatagtg agagcttttt tgcgaatcta ggatgttcaa gacagcggcg tctcaacgaa
     1621 cattcaaata gacttttaag gtgtcatgac ttgccaaagc agacagattt caacgaagtc
     1681 agtcaagaat tttaggctgg acaaaaactc aatttcagga gaaaatgaaa taaatattcc
     1741 cacaataaaa cgcatagtat caaagttttt ccaacacctg atattatgca ttttttaatt
     1801 ttaaagactt tttcccagcc tcaagaaatc acatcccacc taaatccttt ggataccaaa
     1861 tacctcatca ggtattttct gagttggtaa gactgtctag cttaatttga caaatggagg
     1921 gcgttaaatc cagtttttac aggaagtcta tttaaatatt ttcacactga ccttacaaaa
     1981 tatgaaataa attatataaa gtcattccca tgacaaagaa taacatccct ttaaaacagt
     2041 atcttaccca attttcaggt aagacttttg atacaaccgt ccccaatacc cctccgcata
     2101 tagaagcaag aaaaataact ggaacaggtg ctaaatgata ctgatttaaa cctgtaacca
     2161 caacaattgt agccaaatga gacagctggg aaaagaaaat aattgcaaga gaatacatcg
     2221 ttgcttcttt tatcgaaata gaaaaaaaca ccatcaataa agatacattg agcggaccac
     2281 caccaatccc taaaaaacta gaaattaagc ccaatagaag accacaaatc atatagaatc
     2341 ctatcttatc aaattgataa gtttttttaa aatctgttaa aacaaatgat acaagtaaca
     2401 tcacaaatag taaaatcatt tgaacaatag atactagccg tactgataat tgatataaca
     2461 aaacttgaaa aataagatga cctagaaatc ctcctaaaac agaagaaaag gataccgtaa
     2521 aaatcaatct ccattttatc tctttacttt gtgaaaatct tttgattgtt gaaataatcg
     2581 ccattataaa aacagagaaa ctagaataaa aagcaatatc actgaccgaa tggtatccaa
     2641 aactatccat aagtggctta attataattc caccacccat gcctgatata gaacccaatc
     2701 cactagctat tataatgatt agaaaataaa taatctcaac cactttttaa gtccatccta
     2761 ttctttcaaa catctaaaac ctagatggct actagtggaa gtagaaatac atccatttct
     2821 agcagctact cggtatcgat tacaataaga acaatgacat aaaaaggaac ctcctctgat
     2881 agcatactcc tcatcttgta tcccatattt tggtaactca tagtcatctt cattaaaact
     2941 agctaataag gtatatctcg gattgcgaca ccattcccaa acattcccaa tcatttgata
     3001 tagaccaaaa tcattaggta aaaattcata gacaggagcc gttcctataa atccatctaa
     3061 ggcagtattt tcataaggaa atcttccttg ccaagtattg gcatgataat atccaccttc
     3121 taaaagagta tctccccaag gatattcaga ttgtcttcct cctctagcag catattccca
     3181 ctgtgcttca gtcggtaaag acattccgct ccaattacag aaagccaatg catcttctaa
     3241 agcaacatga actactggat ggtcttctaa acccactaga ttactgttct caccgtaggg
     3301 atgtttccag caagcgttcg aaacttgtaa ccaccacgga gccccagcag gatgaggata
     3361 tccctccctt tctgcctccg gaacgaacaa tataaaaaca aaagaccact cttgtcgctc
     3421 agctagtgtt ttatagcctg tttctttcac aaattgagca aattcttgat tcgtaactgg
     3481 agtatccgca atggaaaaat cctttacact aacagcgact tgaggacctt caaaatcatc
     3541 taaaaagccc tcttcagaat ttgttcccat cataaatgtc ccacccttga tttttttcat
     3601 tttcatgatt gactatcccc cgttttcgtt agagttggcg ttattttaga aagttcaaca
     3661 ggaactaact cttcatccac aacaaaacct tcttctctat atctaagaaa gtctactaat
     3721 tttttcttca tctgacgaac aataggttga tatttttcac ttggatacaa atcattcatt
     3781 tcatgcggat cttttttcat atcaaataac tgataatgat ttaaaacagg aaaccagata
     3841 aatttccact ggtctgtcaa aatatattga ctagaatctt ttcctaaagt atgttcacca
     3901 tgaaattctg ttcgccaccc ctcgtactgt ccaaataata aattcttaac actccgtcca
     3961 tctagttcat ctgtagtagt tcctcctgcc aaatccacga gtgaaggaaa aatatcttga
     4021 attttcacta attgcttgat tgttccccta ttaccagcta tcaatccagc cggatcgtaa
     4081 ataaacgaag gaatgtggat acttccctga taaggatatc cctttctaaa cagataatgt
     4141 tcaccaagct gatctccatg atccgaaaca aaccagataa tcgtatcttt atcatgacga
     4201 aattctttta gggcagtcaa aaagcgacta atttgatggt ctatatgagt aatcaatcca
     4261 aaataagcag caaccattct tctttgatca tcttccttta atttccctct taaagcataa
     4321 atagacggaa tttgcttttc taatacttcc caattcccga tatgcaaatc gagaaattga
     4381 ggcaagcgtt ccatatacat atcaaaatag tattttgggg gatttaatgg agcatgtggt
     4441 ttttcaaaag acattttgag aaaaaaagga accgttggat ctcttctttg taaaaatgaa
     4501 atggactcac tcacaaccca gtttgttgga tgtaacttct catctttatc ccaaggtcta
     4561 gcttcccaag aattacaatc cattccgtca tcaatcaaat caacatcata accaacttta
     4621 ccctttaaaa aggctagata gtcactagca taatcaaatt gacttccata agccttatca
     4681 tactttctat caacatgtag atacccatcg tgaagtaaga catgatcaaa cccaagtctc
     4741 tgtctggaag gaaaaacatg catcttacca atacactctg tttgataacc catatcttta
     4801 aagacttttg gaagagtatt agtaaaattc caaggcacct catcttgata accaactcgt
     4861 ccacttttat cttggtctaa gccagtcaat aaagctgccc tagcaggaac gcaagaagga
     4921 actggagaat aggcattttc aaaattataa cctacactag ccatcatatc taatgttgga
     4981 gtgctaacta gcttatcctt actattcaag gataaggcgt ctgctctcat ttgatctaca
     5041 acaatcaaaa taatatttgg ctgttttgtc tgaaccataa aatctccttt ctaatatggc
     5101 aaaagaggca caagaagata tctaccttta ctgcacccct ttctatatca atctctctat
     5161 ataaaacaat aacattcttg ttatgtttta tagaaaaatg aactaaaata tgactaaatc
     5221 gattaggaaa ttcaaatcat tttctagtac tattttagaa gttacagtgt actattccaa
     5281 cttcaataaa ctataaacct ttgtctaata acaattttag tggagataag aaatcctaca
     5341 cctaactcaa tcttacacgt aatctatttc tattttatca caaaaaacgc aagtaagacc
     5401 attaactcaa ttcagtttta tctgccattt tcacaaatgg gaaataagtc aagacactaa
     5461 taatcaaaca aacaactgat aagatgatgg cacgccaatc aaatgctgta gagaagaaac
     5521 catataaaat tggaggcatt acccaagtaa cattttgtgt aacaggtgaa acaagacccc
     5581 agcttgttgc ccagtaagct accgttgcca tgaaaaccgg gctaagtaca aatggtataa
     5641 atagcaaagg attcaagaca actggtaaac cataatttac cggctcacca atattaaaca
     5701 gaactggtgc tagaccaagt ttagcaactt ttcgataatg actgtttctt gaaaaaatta
     5761 aaatagcaag taccaatcct aatcctccaa accagacaaa cgccccaaaa gacccacttg
     5821 tccatatata aggaatcggt tcaccttttt ggaaagcatc cagattcgct aacatagcaa
     5881 ctccaaacag cccttccatg atgggagcca atacatttcc tccatggaga ccaaaaaacc
     5941 agaataactt atttaaaaag atcatcagaa taactgcaaa gaaactttga gacaaaccta
     6001 gtaatggcgt ttgtaacacc ttgtaaaccc aatcaatcaa taagtaattg ctaagtaaat
     6061 ggaaaacata agtcaagatg gctactataa tacatcgcca taaatcctgg aatgatagaa
     6121 gtgaacggct tagcaatcgc agggggaact gaatctggta acttgattac ccagttcttt
     6181 ttcattactt tacagaaaat aatagaggct aaaaatccaa tcatcatggc tgtaaagtag
     6241 cctctggcat taatatggtt tcctggaatc acattcccaa tagttaccat cagattttta
     6301 ccatcaaatg ctagattatc aattccttgt aagatttgat ctaatttcac atctcctaca
     6361 tttgccaaag ggaaactctt tgtaactgta cttccaatcg aaatgacaaa cgaagcaagc
     6421 gataccaaac cagcagaaac tgtatcaacc ttataaatct tagcgatatt cactcccaag
     6481 caatagatga acaataagga aacaattggt atacttccct tgaataccaa attattgatg
     6541 tcaacaagcc actgaaaggt tttcgtaata ctttctaggt gaaattgttg tggtaaatcc
     6601 actagaaaag catttaataa caaagcaatg gaacctgtca taataacagg catagtcccc
     6661 acaaatgaat cacgtaaagc aactaaaaac ttctgattcc caactttcat tgcaattgga
     6721 gaaagttttt cttgcatttt atccaacatt gtattcatag caaattccta atttttttca
     6781 aatcgttcgt atacttctac cacttctttc gccaagtcaa caaaggtcag actcgtcatt
     6841 aaatgatctt gcgcgtgtac catcaagagg ctaattgatg tttcttcccc ctgtgcctct
     6901 tttgtcaaca tttctgtttg agccttatgc gcctgtaata aggcagcatt cgcatcagct
     6961 aatcttcgat tggctttaga gaaatcacct ttttttgctg cctgaatagc ctccatagca
     7021 ttacttttgg cttccccacc atacataatt agccccatta cagattctaa attactttca
     7081 ttcatagcat tctcctcaat cttagtctaa taaactttca gctaaatcta agactttaga
     7141 accattcatc attccgtaat ctgtcatcgg aataaccgcc acaggaatct gtctatcttt
     7201 tagtttttct tgaaagtccc ctagtaaata gcgaacttga ggacctaaaa gcaacacatt
     7261 tacttctttt gttgctacaa tttcttctgc ttcaggagct ggaactgcaa aaatttctgc
     7321 atccaacccc ttatcttctg ctgccttttg catctttgtc actagcagac ttgtactcat
     7381 acctgctgca cacgctaaca taattgttac tttagccata gcttactcct ttatcaccta
     7441 tttactgacc tagccaagcg agatactgta tgtctcactc ctctaattgc cttgctcaat
     7501 gcataaatat tatcgactgt tgccagtccc ccatatcctg cgtcacctat atgttgaata
     7561 tcaactccac aaattttatt tctaagtgca atttccttga tagtatctgt atcagatgtt
     7621 tcttggctag taccaatagc actcagtact agcccaccct tactatgaac gagatcaacg
     7681 acttcacgca actcttggtc atgaaaggct ggaacggttc caactgctgg aacaagtatc
     7741 acatccgcac ctgcttccaa caattgctct gcgacagaaa gctctgccac aggctcattc
     7801 actcctgccc cgtgcatctt acctgcaata atcaaaccag aaaagttttc cttagcagtt
     7861 tgaacagtct taatgatttc tcgattgcta actcctgttc caggatttcc agtcaaacag
     7921 acaaagtcaa aacctaactc ctctattcgc ttcaatgttt caacactagc aacacgaccc
     7981 gcaacaattt cctgtgtttc ctctagcatc tttgcagatg ggtcaatcgg ttccaaattt
     8041 acaccaattg gacaagcaac aagctggtgt aagcgatgaa taacatcatc acctgaacta
     8101 tccaaagcat aaattttagg ctcaaaaaca tcaacacaat tcaataaaat catatcagct
     8161 ccaaaagctc tagcaatttc agaattagtt atatccccaa caaatgtctc acgagtaact
     8221 acattttcag atagaacaac tctaccttca cttgctaaaa tactttgttt taattcttca
     8281 gcattcatct gtaatatctc agatggattt gcactaatta aacgtttcat tctcaatcct
     8341 ccaataactt aaaccgtttt tcaatagcta ttacaaatgg taaataaagt aagacatcca
     8401 aaacaatcaa tacaatttgt aataatgaac cagaaatact tcctgtagca agtaaaccac
     8461 ttataacagg tggcatagtc caacttggat ctgtataagt tagcggaacc aaacctgatg
     8521 ccattgctcc ccacgctact agtaagttaa acattggagc taagataaat ggaactagta
     8581 acaaaatgtt caaaacaata ggaacgccaa acattgctgg ttcatttata ttaaataatc
     8641 caggtataac agttataggt gccaaagttt ttagttgttt actagctttc ttcttatgtg
     8701 ctaaatatcc taacgcaaga actaaaccta tggttgcacc accgccacct atgaatacaa
     8761 aattatccat aaatacgcta gtaaaaatat ttggaagagt ttctccagtt tgataagcta
     8821 ctttatttgc atctagatta gctaaccata acggtttcat aactgcatta acaacatttc
     8881 ctccgtgtaa tcctacaaac caaaaactac tattaaggaa aattaagata agaagaccaa
     8941 taacattatt tcctaaaagt ccaagtggac ctcccaaagc tccttgagca atttcatgaa
     9001 gattaggtaa acttaattta tctaaagttg cataaacaat caaccaacca acaatagtca
     9061 ctgcaccagg aataatggca ctaaagcttt tagatactgc tggtggaaca ccatccggca
     9121 tttttatctg tatattatga tttataaacc attggtagat atagccatta atcaatccta
     9181 aaatcatagc aacaaataaa ccttttgatg ccatgaaagc ggtaggcatt cctgctccag
     9241 cctctcctgt aatgaaaggc gtaactgtaa taaaagatga taatgacaca ataccagcag
     9301 gaataccatc aactttgtat tgtctagcca taaagtaagc gataccaaaa ctagctacaa
     9361 gaccaagtaa tccaaaacta ctatcaacac ccttccataa atagtcagca acaccaatat
     9421 cacctagata tttttcccat cctggaatag gaaaacttgc aataaccatt agtaatgaac
     9481 ctatcaacaa taaaggtata gttagagtaa taccgtctcg aatagaaacc aacgctttat
     9541 tattacccaa tctagccgct actggaagta acttttcttg taataatttt tccataacct
     9601 tttcctctta a
//
LOCUS       DBERCA010000062         9478 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.062, whole genome shotgun sequence.
ACCESSION   DBERCA010000062 DBERCA010000000
VERSION     DBERCA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.062"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(159..914)
                     /locus_tag="ACL719_001724"
     CDS             complement(159..914)
                     /locus_tag="ACL719_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HGS0699652.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(984..3635)
                     /locus_tag="ACL719_001725"
     CDS             complement(984..3635)
                     /locus_tag="ACL719_001725"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGS0699653.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(3628..4206)
                     /locus_tag="ACL719_001726"
     CDS             complement(3628..4206)
                     /locus_tag="ACL719_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0699654.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(4206..4766)
                     /locus_tag="ACL719_001727"
     CDS             complement(4206..4766)
                     /locus_tag="ACL719_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS0699655.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(4753..5088)
                     /locus_tag="ACL719_001728"
     CDS             complement(4753..5088)
                     /locus_tag="ACL719_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699656.1"
                     /translation="MSHQEKLERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDG
                     ICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIK
                     KARKKDGKN"
     gene            complement(5614..6009)
                     /locus_tag="ACL719_001729"
     CDS             complement(5614..6009)
                     /locus_tag="ACL719_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS0699657.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(6105..6359)
                     /locus_tag="ACL719_001730"
     CDS             complement(6105..6359)
                     /locus_tag="ACL719_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGS0699658.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(6369..7703)
                     /locus_tag="ACL719_001731"
     CDS             complement(6369..7703)
                     /locus_tag="ACL719_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGS0699659.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQERFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIADESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(7703..7936)
                     /locus_tag="ACL719_001732"
     CDS             complement(7703..7936)
                     /locus_tag="ACL719_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGS0699660.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPIMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(8398..8580)
                     /locus_tag="ACL719_001733"
                     /pseudo
     CDS             complement(8398..8580)
                     /locus_tag="ACL719_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8582..9361)
                     /locus_tag="ACL719_001734"
     CDS             complement(8582..9361)
                     /locus_tag="ACL719_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699661.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYTF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         2701 a         2151 c         1784 g         2842 t
ORIGIN      
        1 gtaaataata aaagagttat aatcagcact actgataaat tcaataccga tttcgccatc
       61 ttaaaactta gtcaaacgaa taccgttgat ttccagttca ttaagtcgtt gacctatcaa
      121 gacttctcgt tccttcaact ggaaaagtta agggggaact aatacaaatc ctctacgctt
      181 ctatattcaa tgatttcatt tttgataata ttctttttaa actcaaaatg ttttaaagga
      241 ttatcgatta acactaactt tggaatatca tctagatttg tgaccaagga taaaataaaa
      301 tccgattgaa actccttgac tttttcaaat tcttttgcag ttaaacgcac acgcccctta
      361 gattttcgga taccttttac ttcggcaagg taagaatact cttggacatc tacctgaaag
      421 tcatatccat caccataaag acgggcatcg gttaactgtc caccttggaa tttttcttct
      481 ttatcaaaat gaaggataaa ataattctca gcctccattc ccgtttcctg tagacgtttg
      541 aatttagttc gaactattgg tttttcaacg atggttatgc ctgttttctt cccttcactg
      601 gcaagtaaca ttttaactat ttcagcataa ctatgaacat cttcatttcc aaacatcata
      661 tcaattaaat ctttacgaaa acgataaact tcagctttct gccaccaacc ttttcgatta
      721 ttgtcaaaat aaggatcaaa taaatccatt ctatttttta caactccagt tgtttcagca
      781 attcccagag aaaccacata gcgataaaac tccgacttgc tagagcattg aaattctttt
      841 ataaaaagct tgtcaaactt ggcaagaccg tagccaatta aatttaacaa ttcataatga
      901 gggtgtttgg acatcacttt ctccatcaat agatattgat ttcattatac caaaaaacac
      961 ggctcatcac cgtgtttctg tgtttatttc accaacttct tcatctcatc aatacgtgct
     1021 acggtcacat catacttggc ttggtagtcg gcttgtttgt ctttttcttt ttggacaact
     1081 tctggtttag cgttggcgac gaagcgttcg ttagagagtt tcttaccaac catgtccagt
     1141 tctttttgcc atttagcgag ttctttgtcg agacgagcta gttcttcttc aacattgagg
     1201 agatcggcca gtggcaagaa gatttcggct cctgtaataa cgcttgacat agccagttca
     1261 ggtgcaggga tggttgatgc gatttccaag tgttctggat ttgtaaagcg tttgatatag
     1321 ttgacattgc tgttaaagaa ggcttccaag tcgctatcgc ttgtcttaac aaggatggtg
     1381 ataggcttgc ttggtgctac atttacttcc gcacgcgcat tccgaacagc acgaatcaag
     1441 tctttgagac tttccacacc agtgtgagcc gcaaggtctt caaaggctag attaacagtt
     1501 gggtatgcag ctgtcacgat agaaccttct gagatttgtc caaagatttc ctctgtcacg
     1561 aatggcatga ttgggtgaag gagacgaagg atcttgtcca aagtgtatag tagaacagaa
     1621 cgtgtgatga ctttctcttc ttcgttatcg ctataaagga cttccttggt caactcaaca
     1681 taccagttgg caaattcttc ccaaatgaag ttgtaaagga tatgaccagc cacaccaaac
     1741 tcgaacttat caaagttttc agtaactttt gcaatggttt cgttgagatt gtggagaatc
     1801 cagcggtccg tcacattacc agcctcacct gttgcaactt ttgtgacatt gtcatgcgcc
     1861 acatccagcg tcaaacctcc attgttcatg aggatatagc gagaaatgtt ccaaatcttg
     1921 ttaataaagt tccatgaagc atccattttc tcataagaga aacgcacgtc ttgtcctggc
     1981 gcagaaccgt ttgaaaggaa ccatcgaagg gcatcggcac cgtatttctc gataacatcc
     2041 attgggtcaa tcccgttacc gagagactta gacatcttgc gtccttgctc gtcacgaatg
     2101 agaccgtgga taaggacgtt ttggaatggt tgacggcctg tgaattccaa tgactggaag
     2161 atcatacgag acacccagaa gaagatgatg tcgtaacctg ttaccaaggt tgaagttggg
     2221 aaataacgtt taaagtcttc tgagtcgact tcaggccagc ccatggttga aaatggccag
     2281 agggcagaac tgaaccaagt atccaagacg tcttcgtcct gagtccatcc gtcaccttct
     2341 ggagcttctt cgccgacata catttcacca tcagcattgt accaggcagg gatttggtga
     2401 ccccaccaaa gctgacgaga gataacccag tcgtggacat tttccatcca ttgaaggaag
     2461 gtatcgttga aacgaggtgg gtagaattcg accttgtcct ctgtgtcttg gttggcaatg
     2521 gcgttcttag ccaattggtc catcttgacg aaccattgag tagacaagcg tggctcaact
     2581 acgacacctg tccgttctga gtgaccgact gaatggacac gtttttcgat tttgacaagg
     2641 gcaccgattt cttccaactt agcaacgact gccttacgag cttcaaaacg atccatgcct
     2701 gaaaattcaa aggcaagctc attcatagtt ccgtcgtcgt tcatgacgtt gacttgtggc
     2761 aagttatgac gttggccaac caagaagtca tttggatcgt gggcaggtgt gattttcacg
     2821 acaccagtac caaactcagg atctgcgtgc tcatctccaa cgattgggat gagtttatta
     2881 gcgattggaa ggatgacgtt tttaccaatc aagtccttgt agcgcgggtc ttctggatta
     2941 atcgcaaccg caacgtcccc aaacatagtc tcaggacgag ttgtagcaac ttcaagggcg
     3001 cgtgaaccat cttccagcat gtaattcatg tggtagaagg caccttctac atccttgtga
     3061 atcacctcaa tatcagaaag ggctgtgcga gctgctgggt cccagttgat gataaactca
     3121 ccacgataga tccagccttt cttgtaaagg tccacaaaga ccttacgaac agcttttgac
     3181 aaaccttcgt caagagtgaa acgctcacga gaatagtcta cagaaagccc catcttgccc
     3241 cattgttcct tgatggtagt ggcatattcg tctttccatt cccagacctt cgtcaagaaa
     3301 gactcacgac ctaggtcata acgcgtaata ccctcaccac gtaagcgctc ctcaacctta
     3361 gcctgagtcg caataccagc gtggtccata cctggaagcc aaagggtatc aaagccctgc
     3421 atgcgttttt gacggatgat gatatcctgc aaagtcgtat cccaagcgtg accaaggtga
     3481 agtttcccag ttacgtttgg tggtggaatc acgattgaat aaggcttagc cttttgatcg
     3541 cctgaaggct tgaaaacatc cgcatcaagc catttttggt aacgaccagc ctcaacctcg
     3601 gctggattgt atttagatga aagttcttta gacatgtgtg tgtcctttct ctattttgtt
     3661 tattttattt tgaatttgct tagcagcttc ttctgcagac aaattcgtat tatttatttt
     3721 aaagtagtgg tgcaactcat tcggttgata ttgggaattt aattgaagtg tttcagcggt
     3781 ctctaaaatt tctctttcag atacctcaat atgtcgtttt aagggtttgt gctttaatcg
     3841 attctccgtt cgatttcgac gtatgcactc ttcaagactt gtttccaact caacaaaaag
     3901 aatctcttga tgataatcat ccaacaaatt ctgaatttgc tttaaataca tcagctgata
     3961 ctgatttgaa aaatcaatta catctgttaa aattactgat cgctgatttc ttgcacttgc
     4021 tccaaggaat gaaaaagtga ttccacgaac aaattcccac atttcctctg tatagtcctg
     4081 atagatttct agtgcaaaat caatggcttg atggttataa aatagggtag catccgtcag
     4141 tcgagataat tcttgaccaa tggtcatttt tcctgatgct ggagcaccaa tgatgaaaag
     4201 atgcatcaaa tcacctccca ctcactcttc agcaaggcat atctcaaatc atcacagcag
     4261 ttgccttgag catctttacg gtctcttatg cgagcttcga gggtaaagcc aagcttttcc
     4321 gcgactcgtt gactttgaag gttatatcca aagcaagtta gttcaatctt gtgaagaccc
     4381 aaatctttaa aggctaagtc aatcaaggca cgcgcagctt ctggcacata acctcgcccc
     4441 caatagtctg agtgtaaggt atagccgatt tccatcacat catcttcatg gcgatggttg
     4501 aaatagatag agccaacgat cttatccgtt cctttgacga caatcccgta gcctgctggg
     4561 agattttcct tttgattacg ctccggaaga atgtgctcca gataataaat ctcatcttcc
     4621 aaggtcttga ctggaggaaa acctgctgga taggcgacct ctggcaaact agcatagtca
     4681 aagatatcct cagcatccgc cacagtacgg actcgtaaaa cgagacgttc tgtttcgatt
     4741 ttatctggca gctcagttct tgccatcctt cttcctcgct tttttgatga aactgccctt
     4801 catatctaca cgcttgtcca gatagcgata aacgcgctga tatccatctc ccatgaaata
     4861 ggttggggca aacagttgat ttttaaaatg tcccttttca tccaggagtt ctggggcaac
     4921 aagtcgctca agaatcttgg caaagatgtg gcaaataccg tcttcctcaa caatcctatc
     4981 tacccgacaa tctaaaacaa gtggacaggc gtctaaaata ggaatctgag ttcgttcaga
     5041 aatttcataa tgcactccca aacgttccaa tttctcctga tgactgataa aaccagccta
     5101 ctccatcgca agcatagaag tttcatcaga aatattcaca gtaaattttt gatactgttt
     5161 gatctgctct gcggcattct ctctcgcaac gactccaatc acaacccaat ctcctagact
     5221 ataagaggaa ctacaggtcg tgatgttata gccaaaattc taatcttgat atcctaaaat
     5281 aaaaacagga aaaccataat atagtttatt agttttaaat gattgtttta taacaacctc
     5341 ctttgactaa gacacaaaag aaaagccctg ccatctacat gacagggacg aatgtgttta
     5401 tccgcggtac cacccaattt cgggccaagc ccgcatcttt agtctttaaa ataaaaagac
     5461 agaaatattt cattttttca gtcattagca accagttttg acacatttgt ggacttctca
     5521 gcgccgccac tttctgtaaa atgtgggatt caaaacacct ctaatggctc tattgtagca
     5581 agtttttctc ggaaatgcaa ggctcaagaa aaattacttg aacttgatgc cattttcttt
     5641 caatttcttg atggtagctg ggccgattcc tttcaaggca aggagttctt tttcagtcca
     5701 gtttttgaaa tctgaagcag acttgattcc ttcatcatag aaagttttgg cacgatcaag
     5761 tggaagtcca cccaagtttg ctgcaaaatc ttctacagaa ttagctactt tttgagcttg
     5821 ctctttggta gcttctactg cttgttcaac ttttttagaa acagccttac cagctgcgct
     5881 tgctgactgt tctgcacgct tcacgacttt ttttgtctca tctacaacct tagtcacagt
     5941 acttgaaaag gcacctgcgc gacggaggcc atttcgtaat tgttttttac gattgagttt
     6001 ctttgacatg ataaaatcct ttcaataaaa aatccctgct ctgacaagga tttaacataa
     6061 acattctatc aagattttag tgaaaaatca agaatctttc tcgtttaata atttcgctca
     6121 ccaaacaagc cttctggcaa atcatggaat cccttgcctg ctttgaagtt ttcaaactta
     6181 ccaagcaaga actgataagc atccaagcga ctctcgtagc gaatcatggc atctttggca
     6241 cgctcgtcct ggtcttcttc gtaaactttt tgactttcct tgtgcgccat gtcgttaatc
     6301 atgatttggg tattgaccca agcttcaaat tccttcataa attcttgttc gtaactcatt
     6361 ttttctcctt attctaatcc acgaaaatag tccgtatcaa tctcacggta gggctggata
     6421 tcctgaacat actccaacac tccttggaat tctccgtctt catcgtgtac tgcagcatag
     6481 gtgatgtgga caaacttacc tcgcgactca gacttgaacc acatttcata cttgtccttg
     6541 gtcccctcac gaagcccctt catgatagtt ttgaccttgt ccaaatactt aggcggatga
     6601 cagagttcga cattgcgccc gacttgggac ggcgtccgtt tgaaaatcat ctcatcagct
     6661 ggcgtattgt cattgtaata ctggaaaata tcttctttat tgacaaaggt aatctccata
     6721 gggagatgat tgaggatgag attggcctgc tcgactgaaa gatagccatt accaaaagcc
     6781 tgttgactat ggcggtccag cacagcttcc ttttccttag gggtaaaggt aatggtaaaa
     6841 tggccttctg gcgtatctat gacttgttga acttgacctt ctgccgtatc tagctgtaca
     6901 ggctcctctg caatcttttc ctcaataaag ctctgtcgtt ctggcaccca tttctctgac
     6961 ggacggatga tggcatagcc ataggcatcg ctctcatccg caatctgaag ccagtcatcc
     7021 tgagtaaaag actcaaggag aatcatgagg aggatggact cttccttgaa aatcatactt
     7081 tcaaactctg tcgcaaaagc ttcaaaagct tcctttacac tgctaattga cacttctggt
     7141 agtgacttgg ctgtcgttag agctgtttga aagagttccc taatctgatc atccactccc
     7201 cacataactt tgggaggtga atcgtgtcca tagcgctcca tgataggaaa gaagagttct
     7261 tccttacgtt ggtaatggat gtcaaattgc cccacaagtc ccatctgacg caccaagccc
     7321 ttgcgcatct ccgcaagcat ttcctcgtct tccatagact cataggtatc taacaatcta
     7381 cgaatgcgaa tcaaagctgc acggagggcc aaattttctt ccttaaagac acgaactggg
     7441 tgcccagggt gttcggtatc taaaacttcg acacctttga tagcattttt aaaaagattg
     7501 gcatggacat cacagagttc cataacatct tcaaaagtga cgcccgaatc cgagttcatc
     7561 agctcgtgct ccataaggga aatctcgatg gctgacacgc ccgtaaaggt cgcatcaaag
     7621 cgctcttgaa ccgactcagg agaggcgcca ttgtgcaatt ctaacaaaat atcccgtagg
     7681 atatgaatcc gttcatctgt cattagtcta atccaatcac ttcgtagcca ttcgcttcca
     7741 gtgtgcgtac aatcttgtcc ataggagttc ctgctagctt agaaccctgt ttaagtgata
     7801 ctttacgacc aactgtattg cgcattatgg gattggcaag gggtttaaaa cccaactcca
     7861 ctagaatttc caagacttct ggatgcttgt ccaccacttc tgcaacagga attgacacat
     7921 cgatgatatt gtccatgacg acctccattg tatgttctaa tactcaatga aaatcaaaga
     7981 gcaaactagg aagctagccg caggttgctc aaaacagtgc tttgaggttg tagatagaac
     8041 tgacgaagtc agctcaaaac accgttttga ggttgtggat agaactgacg aagtcagtaa
     8101 catatataca gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg
     8161 attttactgc ttatattata ccatatggaa agagaataaa aaactagcag tggagttcct
     8221 gctagttctc tgcgacttgg attaccatct agataagttt aaagagccaa tccaatatct
     8281 taaacactat cttgtaaaac agcaattgga ctgcaaaaga gatgatcatc caaaagaatt
     8341 tcacaatccc aataatcggt ttgataaaaa taatggcaag aagtccccag aaaaatttca
     8401 ttttttatcc tctggcttaa tgagccaccg agccgtatcc attaacttgt cggcaataaa
     8461 aagtttcccc agaccgacaa cggcattatc atctttcttt atcgttttca ttacttttac
     8521 accttacacg gtcaaaaagt aattaccata aattttagct agagttttta tgagtcccat
     8581 attactctcc ttcgatgatt tcagcctctt ttcggattgt ttcaacatct tcttgatatt
     8641 gaacttcact atagttaact agcttggaca attctttctg caagctttca cccatcggtt
     8701 tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga ataaatttcc
     8761 ctattccttt ggccagccct cccttggtaa gattcacacc aaatattttt aagacttttt
     8821 tcaaaatagg gtaccaaagc gtctttgtta aagctttatt aggcactgtt ttcattacct
     8881 gtttggcaat tgttatacca ccagcacgta gcaaagcagc ggttccattc acccccaaca
     8941 tgacgcctag ataaagcaag agggtatttt gagcatcttc actcaactcc tcgcgtgaag
     9001 cccaaagatc ctcataacca taaatataac ctaattcttg agccaatttt agagagaaag
     9061 tataaaattg agccacatca gctggaatgg taattgccat agcaagccct ccaggtaatc
     9121 ctgccaaaac agaagtccca ctcgctaata aaacattatc cctaatacag gcattagcca
     9181 ctcgatctaa gatttcttga gataagagag acgttggacc ttgttccaat aaggtaggaa
     9241 tgtcctgtgg acccaattct ttggaaaact tatccactaa aaatttcgaa cgatcaacct
     9301 taacaacagg tagtttcacc acttgttgca atacttgcat agctaagtct tgttcagcca
     9361 tatacaaact ccttatttct tttttgcacc ttatcatatt atatttccta caaaaaaagc
     9421 attttctaat tagataacat taaaaaatac agaacccctt ttttatagtg aaatgaaa
//
LOCUS       DBERCA010000063         9005 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.063, whole genome shotgun sequence.
ACCESSION   DBERCA010000063 DBERCA010000000
VERSION     DBERCA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.063"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            8..1231
                     /locus_tag="ACL719_001735"
     CDS             8..1231
                     /locus_tag="ACL719_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS0699662.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            2663..2947
                     /locus_tag="ACL719_001736"
     CDS             2663..2947
                     /locus_tag="ACL719_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699663.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            3274..3492
                     /locus_tag="ACL719_001737"
     CDS             3274..3492
                     /locus_tag="ACL719_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699664.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3859..6183)
                     /gene="pflB"
                     /locus_tag="ACL719_001738"
     CDS             complement(3859..6183)
                     /gene="pflB"
                     /locus_tag="ACL719_001738"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGS0699665.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            6531..7592
                     /gene="dinB"
                     /locus_tag="ACL719_001739"
     CDS             6531..7592
                     /gene="dinB"
                     /locus_tag="ACL719_001739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGS0699666.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(7739..8584)
                     /locus_tag="ACL719_001740"
     CDS             complement(7739..8584)
                     /locus_tag="ACL719_001740"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGS0699667.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(8615..8752)
                     /locus_tag="ACL719_001741"
     CDS             complement(8615..8752)
                     /locus_tag="ACL719_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699668.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            8839..>9005
                     /locus_tag="ACL719_001742"
     CDS             8839..>9005
                     /locus_tag="ACL719_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0699669.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIAL"
BASE COUNT         2982 a         1707 c         1608 g         2708 t
ORIGIN      
        1 gaaacaaatg attacaaaac aatcagcctt tttacaaaat agagccacta tactggagtt
       61 tctatatcga aatccagcaa catcccgtac agatattgtt aatgaaacag gacttactcc
      121 agcaacaacg actaacatca taaaagagct aagcgaacag tccctcatat acgaaactgg
      181 tgatgaattt agcgagtttt ccggatctgg aagacgtaga aaaactattt ctatcacaga
      241 taatattccg tatgttgttg gaggtattga aataaacgtc ctaggtatct ttctcagtct
      301 atgtgactta caagggaaaa ctcttttcga gacagaaatt ttgaatgaag attatcctat
      361 ttcagaaatc aattccacca ttaccaatat gataaaaaca gctatagagt acgtcccttt
      421 ggaaacaaaa ttacttggat ttggcttatc aatacctgga cattataaca aagactccgg
      481 aagtatcatt acaaacaacc ccatatggga atcttttaat ttattaaatg taattaaaag
      541 attcaatttt ccttttattg taaaaaataa tatcgattgt atggctatag gacaatacct
      601 ttttaatcca cacaataccc ccgataactt tattttccta cacgctggat taggtattta
      661 cacttccttt ttcacaaaag aaaaaatagg agcctctaaa aatccttata tcggagaaat
      721 tggacacacc attgtcgaat tgaatgggca atattgtgaa tgcggaaaaa aaggttgttt
      781 acaaacatat atttcggatg cttggttaat caaacacgcc caattattat ttaaaaattc
      841 ccaactaact gtactaaaaa gccttgtaaa gactgaaaaa gacattcatt tagacaccct
      901 tttaacggct tataatttag gcgactccgc tttacgtcaa caaattgata aaggagtcaa
      961 tttattagcc acttctattg caaatctcct cctcatcaat cctgctgata aaatctatat
     1021 caacagtcaa ttgcttaatt atcaaccttt cactcatgaa gtcagggata aaatccaaga
     1081 ccagctccac ttcgttccct ttactcgtaa tatagaaatt gaaattttac cttacaacaa
     1141 acatcgtgga agtataggag cttgtgcatt agctatcgtc gcttttttca tagaacatag
     1201 caatgtatta caagatatta tttcacctta atatattaga aatctataga cctgtttaaa
     1261 tcaactataa cctgtagtag atatctcgta tttagacaat atgaaaacaa gacgacttcc
     1321 atataggaaa ccgccttctc tctatgttga gtgatttata ttaaaataac ttttcttcta
     1381 gctgcatttt attattataa aaacattcat cataaccctg aacttaaata acgattttta
     1441 ttcaagatac atactcctag aataaacttt atatgaaatt ctcatttttg tttttacaat
     1501 tctccttagt taaatcttgt ttaatatatg ttttacatat agtatttagc gccacatagt
     1561 actgaactct ctccaaaaac ggttattcct ctttgaatag ggcgttatca caagaaaagc
     1621 atctccacgt ttcaacttca tatggctcaa aaacaatcaa ttgatgctaa aacctgtacc
     1681 tagatgtttc ggttcataaa accatgaaac tgtaaaagtg gatgaaattg atagcgatag
     1741 tcaaatcaag aggcatcata actctaaaaa gtcacaatat ataagttcat cctcggaaaa
     1801 atatcattct aattgttgaa atgcctacat gaaaagaaac gtcaaatgct catgaaacaa
     1861 cgaatacagg tatcaaaact atgacaaaac aagtccctaa atttactaaa gacactgctc
     1921 aactttacac ctgtaaatgg ttgttgtata ataaagttac aaagatgtac gaccacactg
     1981 ttgtaaatca tagtgttcgc gaatatatta ctgatagcat ttctacaaat acaattgaag
     2041 agactggata gactttaaac gaaatatgtc agtgttttgg cattcctagc cttcatatca
     2101 tttaaagaat tctatagaca aaatttttcc caatacagac actcgtaaca actgcttcat
     2161 ttttctacca acatatttag gaacaggata agatagaaga agattaatcc atagctcagt
     2221 tctataccaa tctaagacaa ataagctaaa aaaacgattg ataataagca aatagattcc
     2281 aaattttctc tatctgctca ttttaataaa caatactagt gtaactatcc ttccagtcag
     2341 aaacttgtca aatcacaccg aaaactcttc taaaatttat ctcgttaggc aatcaagcaa
     2401 aaactcgacg atagtacaaa cattatcata cagaattgac tgcctaaatt atatacttta
     2461 gtaaggtttt cggataagaa aaaaggttca ttttacattt ctaaacattc ttttctaaga
     2521 tgaaaaacag aatttttcga ttgtgattta aagcaacaag aagattttca gtatcatcct
     2581 atagatacga gctaattaag aaaaactaca tttttgaata taaactacaa taatataaac
     2641 taaattttat aggaggaaga caatggattg gtacgattat atgatacagg catccaaaca
     2701 atcacaattc aacgcaagcc attggtttcg ctatttgcga aaagttattt ttgaagacta
     2761 ttcttattta acaaaccaag atgtagaaaa gttgctagac tccaaagaac taacccgttt
     2821 tcaaaaaatt agcttgaagt atgcctttca agagcatact ccaactcata aatatgtgat
     2881 ttcattaaat aaacctgcta agttaaccaa tgttcaaaaa ttgatggaga aatacaaaca
     2941 tggataaaat gaaaccggtc ttccaagccc taaataagaa attaattcag gaaaatctga
     3001 ctttaacaat tatggttatg tcttagaata tcatggttta cgtgccacac aagatgttga
     3061 tgctttttat gaccaaaatc agaaaatcaa tgaaattatt gctcgtgtag gaaaacagtt
     3121 taatcttaac atccacgaag agttatggct caacaatcat gttgcaaagc aaatatgaac
     3181 aaacaacctc ccttaagttt atgcgaatcc ttgtactcat ttgagaatct gaccgtttta
     3241 gtagtcccta ttgagtatgt gttaggaatg aagatgatga gtatcagaga gcaggatttg
     3301 caagatattg gtgcaattat taaatacaaa aactttcatt caccattcga tacctttaaa
     3361 tatttaaaag acatgggatt tgatactatt gacctctctg ttctattaga aggttttagt
     3421 tatgcttatg gtatggattg gttagaaaaa ttttttaaag aaaatcaaga taaacttcgc
     3481 gaattttatt aacagtatca aacaagatga ttgtcaatta gccagtcatc ttatttttat
     3541 gatgaaatga aataaaatcg gaacaaatca cttagagaat tccagtcaat ttctagcact
     3601 gtttgagccg tttcagcgga ctatagtaaa atgaaataag aataggacaa atcgatcagg
     3661 acagtcaaat cgatttctaa gaatgtttta gaagtaaagg tgtactattc tagtttcaat
     3721 ctattatatt tcagattcgg tccactattc cttcccaaaa acattgcata ttgcgccgaa
     3781 ggaattgctc atactatccc aagcacaaaa ccctcagtcg tttgaccgag ggttcctttt
     3841 tattattcaa gaacttgatt agctcaatgc atccaaggcg tcatccattg aaagaacttc
     3901 gtggaagaca cgttgtgtca attcagtttt ttgttctgga gtgaggtatt tagtgtttac
     3961 acagtatcca gagatacgta cgataacgtc ttcgcctgac atgatttttt cgtaaacatc
     4021 gttcaagtcc ataacgttca agttaacgtg ttgtccaccg ttttcgaagt aaccatcaag
     4081 gattgttacc aagttatcaa cttgttcatc acgagtctta ccaagagcgc gaggtgatac
     4141 ttgtgtagtc aatgagatac cgtcagctgc ataactaaag tcaaggctag aaagtgagtt
     4201 caagttttgc aaccaaccac ctttagcttt gttagatggg ttagcacctg gtgagaagaa
     4261 ttcaagttta gacaagttca cagaaccatc ttcgttgagg tatacacctt tgtgaactgg
     4321 tgagttacca gtttgtttag agtaagcaac gttagatgtg attgtcaaaa gtgatactgt
     4381 agcttctgcg tctttgtata gtttgtggct acgtagacga gttgtgtaag cttcgatcaa
     4441 ccattctgcc aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt agtcaccgat
     4501 tgtttcgtaa tcgtagatgt agccatcttc gtcacggatt ggtttaactg tagcgtattt
     4561 gatagctgac aatgtatcaa cagtgttagc aaatccacag ataccgaatc ccatgttggc
     4621 acgttgttta gttggcaaga aggccatttg aacagcttcg tagttgtacc tatcagtcat
     4681 gtagtggatg atgttcaagg catctacgta agtgtcagtc aaccagtcaa gagatttttc
     4741 aaagttcgct ttaactgatt caaattcaag aacttcgtca cggattggtt cgatatcaaa
     4801 tactttgtag tctttgtgaa catcgtcgta accaccattc aaaccagtaa gaagggcttt
     4861 aagaacgttt acacgagcac cgaagtactg gatgttgtgg cgttgttctt cattttctgg
     4921 atcaagtgga gacacacagc atgagataca gctcatttca ccatatccgt ctttagccat
     4981 tgttgttaca ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca tacagtagcg
     5041 acggaagttg tatggcaatt tgtcagtcca aagaactgtc aagtttggtt ctggtgagtt
     5101 accgatgttg tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac ggtgacgacc
     5161 gtcgttaccc ataccagcca tagaagttgt gataaaggtt gggtcacctg agtacaattg
     5221 gtcataagct tttgtacgag caaatttaac tgtacgaagt ttcataacga aatcatcaac
     5281 gaattcttgg atttctgatt cagtaaatgt accacgagca aggtcacgtt ctgcaaagat
     5341 gtccaatacg attggtacac gacctagaga tgtagcagca ccgttaatca cacggcagac
     5401 agccatgaaa gcaatgttaa cccattggat tgcttctttc acgttcatcg ctggtttgcg
     5461 aacatcaacc ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt ggtattgaag
     5521 gtttacttct tcacgaagac ggattgtttc ttcatcgatt tctttgattg cattccagtc
     5581 atttactttt tcttgcatca agtagtctgc accgtaaaga gcaagacgtg cgtaaacacc
     5641 gatgatacgt ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg cgtgacgagc
     5701 gcgacgaata tttgaagtgt aggcacggaa aataccgtcg ttaactgttg ttacatattt
     5761 agtgaagatt tcgtgaacag ctgggtctgg ttcgtatcca ttttctttca aagtagtttc
     5821 agccatacgg ataccacctt ttggcatgaa gttcaatttg aagagttcat cgttttggat
     5881 accgaagata acttcatttt ctttgtcgat aaatccagca gggatatcag cgatagatgt
     5941 tggacgagtg tccattggga aacgagtttc ttcgtagtgt gctttagttt cttctacaat
     6001 tttcttgatg tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt ctccatcata
     6061 aggtgtgtag ttagcttgta caaatcgtga tacacttgct ttttctttcc aatctacgcc
     6121 tttgaagcct tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg tcttaacaac
     6181 catattaatg tcctcttttt tctagtaaca gtcatctgtt acattcataa gacaagtata
     6241 ccatatagta accgatttca acaagtgaaa attccctatt tttacacttt cttttctaaa
     6301 acagtctata ttttatttca aactgtatta tatttttgaa aaaataaagt ccttttttct
     6361 ttttttcaga aaaaagggta taataaaaga aaataagcag taacactcaa tggaaatcga
     6421 aaagcaaact aggaagctag ccgcagattg ctcaaaacac tgttttgagg ttgcagatag
     6481 agctgacgtg gtttgaagag attttcgaag agtataaaaa ggtgctaggc atgttgattt
     6541 ttcctttgtt aaatgatttg tcaagaaaaa tcatccatat tgacatggat gccttttttg
     6601 ctgcagtgga aatcagggat aatcctaaac tcagaggaaa acctgtcatt attggaagcg
     6661 accctcggca aacaggtgga cggggagtcg tttctacctg tagttatgag gcaagagctt
     6721 ttggtgtcca ttctgccatg agttccaagg aagcttatga acgttgtccc caggctgtct
     6781 ttatctcagg gaattatgag aaatacaagt ctgtgggact ccagattcga gctattttta
     6841 agcgctatac agatttgatt gaacccatga gcattgacga agcctatttg gatgtgacag
     6901 aaaataaact cggtatcaag tcagcggtca aaattgctcg cctcattcaa aaagatatct
     6961 ggcaagaact ccatctaact gcttccgcag gcgtttctta caacaaattc ttagctaaaa
     7021 tggcgagtga ttatcaaaaa ccacatggtt tgacagtgat tctacctgaa caggctgagg
     7081 attttctcaa acaaatggat atttccaaat ttcatggagt aggaaaaaag acagtagaac
     7141 gtcttcatca aatgggcgtt tttactggtg ctgatttact tgaagttcct gaggtaaccc
     7201 taatagaccg ttttggtaga ctaggctatg atctgtatcg aaaggctcgt ggcattcaca
     7261 actctccagt caaatccaat cgcatccgta aatcaatcgg caaggagaaa acctacggga
     7321 agattctcct tgctgaggaa gatatcaaaa aagagctgac tcttctatca gaaaaagtcg
     7381 ctctcaatct acatcaacaa gaaaaagctg gaaaaattgt cattttgaaa atccgctacg
     7441 aggacttttc aactcttacc aaacgaaaaa gtcttgctca aaaaacacaa gatgctagtc
     7501 agataagcca aatagccctg caactctatg aagaattaag caagaaagaa agaggtgtcc
     7561 gcctattggg gattaccatg actggatttt aaagctcaat gaaaatcaaa gagcaaacta
     7621 ggaggctagc cgcaggctgc tcaaaacact gttttgaggt tgcagataaa gctgacgcgg
     7681 tttgaagaga ttttcgaaga gtataaaacc ttgaagagtt gccccttcaa ggtttttctt
     7741 atacaaataa acggacaaaa ctgtaaagta gcaaaacact accaagcacg atacggtatt
     7801 taccaaaaag ggtgaagtcg tgttttttca catagctggt caagaagcga atagccacca
     7861 tgctgaccgc aaaagctact cccatcgcga ccaagagcaa aaacaattgc ccaaagctca
     7921 agagttctcc ggctttcaca aatttgaaaa tctttaaggc actagctcca aacataacag
     7981 gaatcccaag atagaaggta aattctgtca caactgaacg actggttcca tttaacaaac
     8041 caccgacaat cgttgcacct gaacggctag tccctggtaa aagagcaaga acttggaaga
     8101 gtccgatata gaaagcggtc gtataaggaa gcttgtccaa ctctgttaca cttggctcga
     8161 tagcacgcgc tttattgcgc ttttccaaat agatgaaggc aaccccgtag ataatcaaca
     8221 tgagagcaac tgaaaccatg ttatggaagt gggtatcaaa ccaatcatca aatttaaaga
     8281 caccaagtaa aggtaaagta gcaatcaaga ccttcaacca tagtctccaa gtcttacgaa
     8341 cttcctgttt gtccttagtc ggtttaaaag gattgagctt gttaaaataa atcaccataa
     8401 ctgctaaaat agcaccaagc tgaatcacga cattaaacat ggacataaag gcttcatttt
     8461 gattttggta ttggatgaat tcctctgcta aaatcaagtg acctgtactg gaaatcggca
     8521 accattccgt aattccttca acaatcccga agaagataga ttttaaaatt tcaataagat
     8581 acatagatta ctccctatat tataattagc gcagttagct actgccaaag cccaagtggt
     8641 atacttggaa taagccactg tggattagtt catttcctcc aatcgcttga cgatttccga
     8701 aaggctctaa ccacagcttt tgtttcaatt ggtaatgagt ttgataacgc atagacgttt
     8761 gatctcgtct aaggtaacaa ggcaaaaaat tcgtggataa ccaagtagac ttgaaatact
     8821 atatctggag gtactcccat gctttatgtg ggcattgata tcgctaaaaa taaacacgat
     8881 gttacagcct tgaatgttcc aggaaaaact gttcttaaac cactcacttt ttcaaataat
     8941 aaagctggtt ttgaactctt agatctgtct cttcgacagc tcaaccaaga ctgtctcatc
     9001 gctct
//
LOCUS       DBERCA010000064         8986 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.064, whole genome shotgun sequence.
ACCESSION   DBERCA010000064 DBERCA010000000
VERSION     DBERCA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.064"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..662
                     /locus_tag="ACL719_001743"
                     /pseudo
     CDS             1..662
                     /locus_tag="ACL719_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(790..1038)
                     /locus_tag="ACL719_001744"
     CDS             complement(790..1038)
                     /locus_tag="ACL719_001744"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699670.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(986..2068)
                     /locus_tag="ACL719_001745"
     CDS             complement(986..2068)
                     /locus_tag="ACL719_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS0699671.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2194..2976)
                     /gene="gatD"
                     /locus_tag="ACL719_001746"
     CDS             complement(2194..2976)
                     /gene="gatD"
                     /locus_tag="ACL719_001746"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGS0699672.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2976..4319)
                     /gene="murT"
                     /locus_tag="ACL719_001747"
     CDS             complement(2976..4319)
                     /gene="murT"
                     /locus_tag="ACL719_001747"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGS0699673.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGTTQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4418..5734)
                     /locus_tag="ACL719_001748"
     CDS             complement(4418..5734)
                     /locus_tag="ACL719_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGS0699674.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5928..7154
                     /locus_tag="ACL719_001749"
     CDS             5928..7154
                     /locus_tag="ACL719_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGS0699675.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7353..8927
                     /locus_tag="ACL719_001750"
     CDS             7353..8927
                     /locus_tag="ACL719_001750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0699676.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2613 a         1730 c         1983 g         2660 t
ORIGIN      
        1 cgggttcatt tagaactaag aaatcgtggt tatctggtaa atcataaaag agttcaaggc
       61 ttgatgaaag tactcaattt acaaactaaa atgcgacaga aacgaaaata ttcttctcat
      121 aaaggagacg ttggtaagaa ggcagagaat ctcattcaag gccaatttga aggctctaaa
      181 acaatggaaa agtgctacac agatatgaca gaatttgcca ttccagcaag tactcaaaag
      241 ctttacttat caccagtttt agatggcttt aacagcgaaa ttatcgccta taatctttca
      301 acttcaccca acttagaaca agtacaaaca atgttggaac aggcattcac agagaagtac
      361 tacgagaata cgattctcca tagtgaccaa ggctggcaat atcaacacga ttcttatcat
      421 cggttcttag agagtaaggg aattcaagca tccatgtcac gcaagggaaa cagccaagac
      481 aacggtatga tggagtcctt ctttgggatt ctgaaattta tggctatgag aaaacattta
      541 aatcacttaa tcaattggaa caagctattg tagactatat tgattactac aacaataaac
      601 gaattaaggt aaaactaaaa ggacttagtc ctgtgcagta cagaactaaa tcctttggat
      661 aaattaattg tctaactttt ggggggcagt acattttaac acttgataat atgcgtttta
      721 ttgtgggaag attttcctca ttttctagtg aaattgaact ttttctaaca tcttttgata
      781 gatgacttct taatcaatta tcatttccat tgttgtttct tggggtttca tcccttgtct
      841 ttgataaaag cggagagcac cctcgttatc attccagaca cttagggtta gattattgca
      901 gccaatttct ttagcataat agcagaaaaa tgacaataaa atagccccta gatattttga
      961 aaggaaagca taggggctat atagtttaat caaaataaag caaatctttg ctggtgcttg
     1021 taaagaggtc gaagccatcc ttggtaacaa caccgcagtc ttcaatacga acaccgactt
     1081 taccagggat atagatacct ggttcaacag agaagcacat gccttcttcg atgaccatgt
     1141 cgtttccttc catgatagat gggaattcat ggacatccat accgatacca tgcccgagac
     1201 ggtggttgaa gtactcacca taaccagctt tttcgatgat ctcacgggca gcgcggtcca
     1261 cttcatgagc agtcacacct ggcttgataa agtcaagagc agcttgttgg gcttcaagag
     1321 tcaagttgta aatatctttc ttgaattggt ctggtttgcc gacagcgact gtacgagtca
     1381 tatctgacgc atagccattg accagaaccc ccaggtcaaa gaggagaaga gcatcatttt
     1441 caaccttatt agctgctgga atgccgtgtg gattcgcagc attatcacca gtcaagacca
     1501 tggtatcaaa gctcatttcg taaccttcac gtttcatggc aaagtcaatt tgtgcgatga
     1561 tatctgtctc agtcttatca agagaaatat tgtcaaaacc aacatgaaca gccttgtcag
     1621 cataaagacc tgcaaccatc attttttgta cttcatcagc tgatttgatg aggcgcatgc
     1681 gttggatacg aggagtgagg ttttcaaact cagcagtttc aaaaattgtt ttcaaaccat
     1741 ggtatttggt caagatgaga ttgtcaaact caacagcgac acgtttgaag tcaagttgtg
     1801 gaagagcatg tttgattttt tgccatggat tttcagaatc gacatagccc actactggga
     1861 aggaaacggt gctacttgca cgttctactt caagagctgg gacaaagagg agaggttcct
     1921 gatccgctag gacaaagagg aacatttggc gttcatgggg atcactgtaa aaaccagtga
     1981 ggtaattgat tgtgacgggg tcagatacga cagcgacgtc tagtttttct gattcaagat
     2041 atgttaggat ttgttgtaat ttagacatat gctaccttct ttctacccct ctattttggc
     2101 aaaaaatgag agaaaatgca agggagaagg gtaaaagaaa agacaaaaaa ctccgacaag
     2161 ataacggagt tctttgatat cattttcctt tgtttaagaa aagtcagcct tgcttttgac
     2221 gtcactgtac tcttcagcga tttcttggct gaggatatcc tcataggcag ggagttggat
     2281 atcctgacca tatttcttct tgagggcagt agtaactagg cgataagcca gattggcatt
     2341 acgagagagg ataggcccgt ggaagtagga accaaagaca ttcttataat gaaccccttc
     2401 accgaccttt tcttcgttgt ttccatttcc atagacaact tgtcccagcg gtttttggtc
     2461 atcagagagg aaggtacggc cttggtgatt ttcaaatcca tagtaggttt catcgaaatc
     2521 ttcattgtga atcttgatgt caccgataaa acggttattg gtctggttga gcgtgtagtg
     2581 tcccatgacc cctagccctt cgatacgttt tcctgaagct tcaacataat attgacccaa
     2641 tagttggaaa ccaccgcaga tagccagaac tacaccgtcg ttttggatgt agttgtcaat
     2701 gctctctttt ttagcaggta ggtcgtctgc aatgatactt tgttcaaagt cttgaccacc
     2761 accgaaaaag gcgatgtcgt agtgattttc atcaaagtca tcatggagag aaacgatgtc
     2821 aacggtcaca tgggctccca gtttttcagc cacatacttg agcatgagga tgtttccatt
     2881 gtccccgtag gtattcatga gatttccgta gaggtgggca atgttgagct gataggggta
     2941 attgccatct tttgaggaaa gtgaagtata aaccattagt tcatctcctt tctaacaatc
     3001 tgacgactag ccagcagttc acgaaattcc agcatggcag tataagttgc cagaatatag
     3061 gcatgcttgc agtcttgatt ctcaatggtc ttgagaactt gctccagatt actcgtttca
     3121 gtgattttct cagctggata gccagtcact cggaggcgac gagcgatttc agaatgacga
     3181 acaccgccag cgttgatttc aggaatgtcc atgtcagtga tttgctcaaa gtctgcatcc
     3241 cagatccagc tagtgtcaat tccatctgca tagttggcat taaggaggac agataggcta
     3301 aatggataag gtgctagttt gatcatttcg atagcttggg ttgtaccgac tggattttta
     3361 atcaagacaa gggtacattc cttgtcaccg atatgaaagg tttcttggcg tccaaagaca
     3421 gcacggctct tgtcaaatcc ctgtttgatg agttgcgaat cggcacctag gaaacgggcg
     3481 atggccacag cagctagggc gttatatata ttatagagcc cgccgatttg gataccgtat
     3541 tcttggccgt ctatgacaaa gcgagagcga ttgttggtca actcaaccaa ttttgtcaaa
     3601 cgatagtcga gatcaggacg tttacatcca caaccttcac agatataggc acccaagttt
     3661 gcataggtat tatgctcata tttgaggatg ccttggcagt caggacagag aatcccttcg
     3721 gtattgtagt gagccagttg ggctggtcct ttttccaagt caaaaccaaa atactctata
     3781 gggtttggaa tagttggctt gtagaaaagt ggactgtctc cgttaaggag aacagtagca
     3841 gttggaactt tccgaatggc atccaatatc atgttatagg tagtatagat ttcaccgaaa
     3901 cggtccatct ggtcacggaa gatattagta atgacaaaaa gactaggctg gatatagtca
     3961 cagatacgag atagactggc ttcgtcaatt tcgaggacgg caatattttt tccagtttta
     4021 gaagatttgg ctgttaggaa ggttgttgca atccctgtaa tcatgttggc accgcttggg
     4081 ttggttagaa cttgaccata aacctctttt aaaatgccga cagtgagggc agttgtcagg
     4141 gtttttccat ttgttccagt gacaacgaca atctcgtagt tcttagctag gttttgtaaa
     4201 atatctttat caaattgaag ggcgacttta cctgggagcg tacttccacg tccaagacgg
     4261 cttaaaacga agtgggaaga acgcccagca agaaggccca aagtagtttt taagttcatg
     4321 tttctattat atcacaaaag agggaaaaga cagatttggt gtttcgtaga agtaaaaaca
     4381 gcccaaagag ggctgtcagt taagatgaga tttcaactta aatcgcaaac aagtcattca
     4441 agttctcagc caaggttggg tgagtgaaga tttgttttgt gaagtaagtg taaggaatct
     4501 tgttgtccat agcaacagta atgatgttga tgatttcttg agaaccttct gagaagatgc
     4561 ttgcaccaag aatttctttt gtttcagtat taacaacagc tttgaaagct ccgcgaaggt
     4621 ctccatttac gtgaccacga ggcatggctg caacagggat ttctttcact gcgtatggaa
     4681 gtttcaaatc agctgcttgg ctttcagtca aaccaacttg tgaaagtgca ggtgtgatga
     4741 acatagtatt tggcacattg agacggtcct caagtgtgta gctgccatct ccagcaaggt
     4801 agctgtaaac aacacggaag tcatcaagtg aaatgtaagt aaattgaaga ccaccgttga
     4861 catctccaac tgcaaagaca ccaggaacgt ttgtttgaca gtgtttatct actttaatag
     4921 cgccacgttc agttagttca atatctgtat tttcaagttg aagtggttct acatttggtt
     4981 tgcgtccagt tgcgtagaga agggcgtcga aacggtaagt ttcgtcttca gttacgacaa
     5041 gcacttggtc accgtcgttt ttaatttcag tagtatggat attttgaagc aattcaatac
     5101 cgtcttcttc catgtattgt ttagcaagag ctgcgatgga aggttctgca cgaggtagga
     5161 atgtatccaa ggcatctagg actgtaacct tgcttcctag tttattgtaa aggccagcaa
     5221 attcaagacc gatatttccg ccaccaagga ctccaagttt ttcaggcaat ttatccaagc
     5281 tttggatacc tgttgagtca aagacgtttt tgcttgtagc aagtccaggg attggcaaga
     5341 cgtttgaaac agcaccagtg ttgatgacga tagtttcagc agtcaattct tgtttttcat
     5401 caccagcttg gatttcgatg actttatttg aaaggaagtg agcttcagca tcaaagatat
     5461 tgacgccagt accagcaaca gtcgtatagt ttttaccgtt gaggcgacca gtgatcgtgt
     5521 ttttggtagc aatgacttct tcaaaagaca agtctttctc agcagcaacc agcaaagttt
     5581 tagttgggat acaaccgatg ttgatacaag ttccaccgta catagctttg ctacgttcaa
     5641 cgagggcaac ttttttgcca gctgaagcca atttacctgc tagtgtttta ccagctttac
     5701 caaatccgat aacgattaaa tcataagtta acatttagag ttcctcctat tcgcatttca
     5761 ttctagcaca agaaaaaaac ttttgcacaa ttctgctacg gaaaagacag aaaaagataa
     5821 aaacgctttc cagacaaaaa actagtaatt ttttgacaag cttgataaac taaaatccta
     5881 tctattttta tataagatag aaaaaatgtt cgaaaatagg aggttttatg aaatcgaatc
     5941 gttatattat tgcctttgct ggggtcattt tacacttaat gctgggttcg acttatgctt
     6001 ggagtgttta tcgtaaccct attattgaaa aaacgggatg ggatcaggct tctgttgcct
     6061 ttgcctttag tctagcaatc ttttgtttgg gcttatcggc tgcatttatg gggcgtttgg
     6121 tagaaaaatt tggtccgaga gtcatgggga gtctatctgc ttttctatac gcaggtggaa
     6181 atatcttaac aggatttgca atagaccgtc aggaactgtg gttgttgtat ctagcttatg
     6241 gcattttagg tgggcttggt ttgggagcag gctatattac ccctgtgtca acgattataa
     6301 aatggtttcc tgataaacgt ggtctcgcaa caggtttagc gattatgggg tttggttttg
     6361 cttctttatt gactagtccc atagcgcaac atctcatcgc aggggtaggg cttgtagaaa
     6421 ctttttatat tttaggtgca agttacttta ttatcatgct cctagcttca caattcatta
     6481 agcgtccaaa tgagcaagag cttgcaattt tatctgcttc agggaaagaa aaaacagact
     6541 ctctgacgca agggatgact gcaaatcagg ctctaaaaag caatcggttt tatatgcttt
     6601 ggattatttt ctttatcaac atagcttgtg gtttaggctt aatttcagcg gcatcgccaa
     6661 tggcacagga gatggctggc ttgtctacaa gtcatgcagc agtaatggtg ggtattttgg
     6721 ggattttcaa tggatttggt cgcttgctct gggcgagttt gtctgactat atcggtcgcc
     6781 ctctaacctt tagtatatta ctgcttgtca atcttttctt ttctctctca ctttggctct
     6841 ttacagattc cgttttattt gtagttgcta tgtctatttt gatgacttgc tatggagctg
     6901 gtttttcttt gattccagct tatctcagtg atatttttgg aaccaaggaa ttggccgctc
     6961 tgcatggtta tattttaaca gcttgggcaa tggctggttt agcgggacct attttattag
     7021 cagagactta taaaatggct cattcgtaca cacaaacctt gttcgttttt ctcattttat
     7081 acagtatcgc cttggctttg tcttattatc taggtcgttc aatcaaaaaa gaaagtcaaa
     7141 aaccgcttac atgattttct gtagaaatgg ggctatcttt tgctctggtc ttgtgttata
     7201 ctagataggt tgcaaagaaa acagtacttt tcttttgtgg aaaagaagca acacgatttt
     7261 atatctagtt atatgaaaat acgtcataaa aagaaaagta taaactaagc cctatagggt
     7321 ggcttcgcac cacctttaga aagaagaata acgtgaaatt taatgaatta aacttgtctg
     7381 ctgatttgct agcagaaatt gaaaaagctg gttttgtaga agctagtcct atccaagaac
     7441 aaactattcc tttggccctt gaaggcaagg atgttatcgg tcaagctcag actggtacag
     7501 gaaaaactgc agcctttggc ttgcctaccc ttgaaaaaat ccgtacagaa gaagcgacta
     7561 tccaagcttt ggtcatcgct ccaactcgtg aactagctgt ccaaagtcaa gaagaactct
     7621 tccgctttgg tcgtagtaag ggagtcaaag tccgttcagt atatggcgga tcaagcattg
     7681 aaaaacaaat taaggctctt aaatctggtg cccatatcgt ggtgggaact ccaggtcgcc
     7741 tcttggactt gattaaacgc aaggccttga aattacaaga cattgaaacc cttatccttg
     7801 acgaagcgga tgaaatgctt aacatgggct tccttgaaga catcgaagcc attatttccc
     7861 gtgtacctga gaaccgtcaa actttgcttt tctcagcaac tatgccagat gccatcaaac
     7921 gtatcggtgt tcagtttatg aaagcccctg aacatgtcaa gattgcggct aaggaattga
     7981 caacagaatt ggttgaccag tactatatcc gtgttaagga acaagaaaaa tttgacacca
     8041 tgactcgtct catggatgtg gcacaaccag aactcgctat tgtatttggt cgtaccaaac
     8101 gccgtgtgga tgaattgact cgtggtttga aaattcgtgg cttccgtgca gaaggaattc
     8161 atggcgacct agaccaaaac aaacgtcttc gtgtccttcg tgactttaaa aatggcaatc
     8221 ttgatgtttt ggttgcgaca gacgttgcag cgcgtggttt ggatatttca ggtgtgaccc
     8281 atgtctacaa ctacgatatt ccacaagatc ctgagagtta tgttcaccgt atcggtcgta
     8341 caggtcgtgc tggtaagtca ggtcaatcta ttacttttgt tgctccaaac gaaatgggtt
     8401 accttcaaat cattgaaaac ttgactaaga aacgcatgaa aggtctcaaa cctgcaagtg
     8461 tagaagaatc cttccaatca aaaaaacagg tagctctcaa gaaaatcgaa cgtgattttg
     8521 cagatgaaac cattcgtgcc aactttgaga aatttggtaa ggatgctcgc aaattggctg
     8581 ctgagtttac tccagaagaa ttggcaatgt atatcttgag tctgacagtc caagacccag
     8641 atagccttcc agaagtggag attgcacgtg aaaaaccact accgtttaaa ccatcaggta
     8701 atggtttcgg tggtaaagct aagggaggtc gtggaggccg tcgtggggac gaccgtcgag
     8761 agcgtgatcg ccgtggcaat ggtcgccgtg atgagttcaa aaaaggaagt cgtggcaacg
     8821 atcgttttga taaggaaaaa cgttaccgta aggataataa aaaaccacgc aatactttaa
     8881 gcgaaaagca aacaggcttt gttattcgta acaagggcga taaataagaa aaagcggttc
     8941 aaaatgaatc gcttttttat ataaggagac cgaatgtgaa aagaca
//
LOCUS       DBERCA010000065         8838 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.065, whole genome shotgun sequence.
ACCESSION   DBERCA010000065 DBERCA010000000
VERSION     DBERCA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.065"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1273)
                     /locus_tag="ACL719_001751"
     CDS             complement(<1..1273)
                     /locus_tag="ACL719_001751"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HGS0699677.1"
                     /translation="MTKKSITRNFIFNTILTMSSFIFPLISFPYVSRVLMADGLGRVT
                     FASSIIDYALTFAMLGMPLYGVKVCAGYIKNKEKLSQTVRELLVINLLVGFLVMLLLA
                     TMVIIIPRFRQEWQLIIITSSTIPLNIIGIEWLYKALEDYSYISIRTLTFKILGFLLM
                     FLVVRTKDDYMVYAAITVLASHGSFILNFFRSRTFLLENISQRLNLKQHIKPLLILFF
                     LSVSWTIYKNTDVVMMGFLTSDTEIGYYSTALKIRSIVLSVVTSLGTVVLPRLVKYYK
                     EGKYNEAKKILNKSSSFIMLSSLYFIGYIVINAREIILFIAGRNYLGAIPTLQVSIFS
                     AIFVGYSIMYGTNILVSIGKENVTIQASIIGVVLNICLNFIMIPKFAALGAGIATSIG
                     EAVMVLYEIIYLGKDGWSYFERLNILKIIVVF"
     gene            complement(1273..2337)
                     /locus_tag="ACL719_001752"
     CDS             complement(1273..2337)
                     /locus_tag="ACL719_001752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0699678.1"
                     /translation="MTKVLQIGPSINLSKGGMSAVIRDLTYSPLLSGDFDITAYQSFI
                     DGSLVTRLTYSSYALLKFVVHSGNYDIYHIHTATRGSCWRKLLYLKLLKSKNKKAILH
                     IHGAEFQIFYDSLPEYKKNKVREMLELSDYVIVLSQTWYDFFSNININAKIVIVENGV
                     DTSFYVEKKKSITSNNFLFLGRMGKRKGAYDLIDAMNQAVAINPNLHLTMAGDGELED
                     IRQKISNLNLTDHITIYDWVNQRDKKILFQANQTLILPSYNEGLPMAILEAMASGLAI
                     ISTPVGGIPEIIHEDNGWLIQPGDISQLSNIILEASYNPDVVSLMGSNNHKLVEEKYS
                     FHSMHGKIKKIYNTLLETKK"
     gene            complement(2350..2829)
                     /gene="pssE"
                     /locus_tag="ACL719_001753"
     CDS             complement(2350..2829)
                     /gene="pssE"
                     /locus_tag="ACL719_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HGS0699679.1"
                     /translation="MIFITVGTQKHPFNRLLKKIDQLIELEVIKEEVFAQIGASDYKP
                     KYFSYNSFLSSIDMNEKMREASMVITHGGTASIVKALKLRKKIIAVPRLEQFGEHVDN
                     HQLQIITVLGEEGYILPCFDIEQLGTVYQKAQTFTTKPYISNSYALLEDIRQYIDSL"
     gene            complement(2830..3276)
                     /gene="pssD"
                     /locus_tag="ACL719_001754"
                     /pseudo
     CDS             complement(2830..3276)
                     /gene="pssD"
                     /locus_tag="ACL719_001754"
                     /inference="COORDINATES: protein motif:HMM:NF041549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
     gene            complement(3313..4680)
                     /locus_tag="ACL719_001755"
     CDS             complement(3313..4680)
                     /locus_tag="ACL719_001755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS0699680.1"
                     /translation="MNGKVVNSSLAIIHSFLVILLTYLLSAVREAEIASATAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVIFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSVVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMCVDAEDKKRELMEQNTM
                     QGGMFKVDDDPRITKVGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            complement(4695..5384)
                     /locus_tag="ACL719_001756"
     CDS             complement(4695..5384)
                     /locus_tag="ACL719_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGS0699681.1"
                     /translation="MPTLEISQAKLDFVKKAEEYYNSLCMNLQLSGDGLKVFSITSVK
                     LGEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVNKYGSY
                     GNYGDYGKNKK"
     gene            complement(5394..6086)
                     /locus_tag="ACL719_001757"
     CDS             complement(5394..6086)
                     /locus_tag="ACL719_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HGS0699682.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKSGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVTVPTDTRIVSISVKDKQPEEASRIANSLREVAVERIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELFDTRVKRPEDVEDVLQIP
                     LLGLVPDLDKMK"
     gene            complement(6095..6826)
                     /gene="cps4B"
                     /locus_tag="ACL719_001758"
     CDS             complement(6095..6826)
                     /gene="cps4B"
                     /locus_tag="ACL719_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGS0699683.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAESFLQVREIAKEVADDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALGNNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQNLVHVIASDMHNLDGRPPHMAEAYDFVT
                     QKYGEAKAQELFIDNPRKIIMDQLI"
     gene            complement(6828..8273)
                     /gene="cps4A"
                     /locus_tag="ACL719_001759"
     CDS             complement(6828..8273)
                     /gene="cps4A"
                     /locus_tag="ACL719_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGS0699684.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLVAYKSLIAGETKAIVLNSVFENIIELEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTSRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVLLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EVLKNYSTIINSLQDSIQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYMMEIDDSSLAVVKAAIQDVMEGR"
     gene            8525..>8838
                     /locus_tag="ACL719_001760"
     CDS             8525..>8838
                     /locus_tag="ACL719_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS0699685.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVDRDRLKNYLTDNPDAYLTEIVSEFGCHPTTIHYALKAMGYTR
                     KK"
BASE COUNT         2853 a         1816 c         1348 g         2821 t
ORIGIN      
        1 taaatactac aataatcttt aaaatattta atctttcaaa gtagctccaa ccatcctttc
       61 ccaagtatat tatttcatat aataccataa cagcttctcc tatagaagtt gcaatacctg
      121 cccctagtgc agcaaatttg ggtatcataa taaaattcaa acaaatattt aaaacaacac
      181 caataatact tgcctgaatg gttacatttt ctttacctat agatactagg atattggttc
      241 catacataat agagtagcca acaaatattg cactaaaaat agatacttga agtgtcggga
      301 tagcccctaa gtaattccta cccgcaataa aaagaataat ttctctggca ttaattacaa
      361 tatagccaat aaaatataaa gaacttagca taataaatga agaacttttg tttagtattt
      421 tctttgcttc attatacttt ccttctttat aatatttgac taaacgaggt agtacaactg
      481 tccctaaaga agttactaca ctgagcacta tagaacgaat ttttaatgct gttgaatagt
      541 aaccaatttc tgtatcacta gttagaaatc ccatcataac gacatctgta ttcttataaa
      601 tagtccatga tacagataaa aaaaataaaa ttaataacgg ctttatatgt tgctttaaat
      661 tcaatctttg agagatattt tctagtagga aagttctaga tctaaaaaaa ttcaaaataa
      721 aagaaccatg actagctaaa actgtaattg ccgcatatac catatagtca tcttttgttc
      781 ggacgactaa aaacatcaaa aggaaaccta atattttaaa agtcaatgtt ctaattgaaa
      841 tatacgaata gtcttccaat gctttgtata accactctat tcctataata ttgagaggaa
      901 ttgtagatga cgtgataata ataagctgcc attcctgtct aaaacgagga attattatta
      961 ccatagttgc taaaagaagc atcacaagaa atcctactaa taaattaatg actaacaatt
     1021 ctctgacagt ctgacttagc ttctccttat ttttaatata tcctgcacag actttaacac
     1081 catataatgg catccctaac attgcaaatg ttagggcata atcaataatt gaagaagcga
     1141 acgttactcg tcccaaccca tctgccatca gaacccttga aacatatggg aaactgatga
     1201 gtggaaaaat aaaactggac atagttagaa ttgtattgaa aataaaattt ctagtaatag
     1261 attttttagt cattattttt tcgtttctaa tagagtatta taaatttttt taattttacc
     1321 atgcatacta tgaaaactat acttctcttc aaccaactta tgattattgc tccccattaa
     1381 acttacaaca tcgggattgt aacttgcttc taaaataata ttagataact gagaaatatc
     1441 acccggttga attaaccagc cattatcctc gtgtataatt tcaggtatgc cacctacagg
     1501 cgttgatatg attgctaatc cagaagccat agcttccaat attgccatcg ggagcccctc
     1561 attataagaa ggcaatatca aagtctgatt agcttgaaat agtatttttt tatctctctg
     1621 attaacccaa tcatatattg taatatgatc agtcaaattc agatttgata tcttttgacg
     1681 tatgtcttca agttctccat ctccagccat tgtcaaatgc aaattaggat ttatagccac
     1741 tgcttgattc atggcatcta ttaaatcata ggctcctttt cttttgccca tccttcctaa
     1801 aaaaagaaag ttgttgcttg ttatactttt ctttttttct acataaaaag aagtgtctac
     1861 accattttca actattacaa tttttgcatt tatattaata tttgaaaaaa aatcatacca
     1921 agtttgggaa agtacaatca cataatcaga taattctaac atttctctaa ctttattttt
     1981 tttatactca ggaagtgagt cataaaatat ttgaaattct gctccatgaa tatgcaaaat
     2041 agctttttta tttttacttt taagtaattt caaatacagt aattttctcc aacaactccc
     2101 tcttgtagca gtgtgaatat ggtatatgtc ataattacca gaatgaacta caaatttaag
     2161 aagagcataa ctagaatagg ttaaccgtgt tacaagagag ccatcaataa aagattgata
     2221 agcagttata tcaaaatcac ctgacaataa cgggctatat gtcaaatctc taataacagc
     2281 gctcatacca cctttagata aatttatact aggcccaatt tgcaaaactt ttgtcatcta
     2341 aatttccttt caaagactat ctatgtattg tcgtatatct tctaaaagtg cataactatt
     2401 agaaatatat ggctttgttg taaaagtctg agctttttga taaacagttc ctaactgctc
     2461 tatatcaaag catggcaaaa tataaccttc ctcaccaaga actgtgatga tttgtaattg
     2521 gtgattatcc acatgttctc caaactgctc taatcgtgga accgctataa ttttttttct
     2581 taatttcaaa gctttaacaa tactagcagt cccaccatgt gtaattacca tactagcttc
     2641 tctcatcttc tcattcatat caattgaact caaaaatgaa ttataagaaa agtatttagg
     2701 tttataatca gaagctccaa tctgagcaaa cacctcttcc tttatcactt ctaattcaat
     2761 aagttgatct atttttttta acaagcgatt aaaagggtgt ttttgagtcc ctacagttat
     2821 aaaaatcacc taataaagcc cccctttata aacagcattt gggtagatat ctagcatact
     2881 ctcccattgc acatagaatc tatctgcgta acggtatagt attctacctg ttaaagtagg
     2941 agtggtcact tttgcaaaag attcaatata aattaattta gccccaaaca tttttgaaag
     3001 caaacaaaac ggtatcattg caagaacacc tgtacagata actacttttg ggcgtattct
     3061 taacagtata taaagtgatt tcaaggaaat ccaaatcaat ttaacgatcc aaaaaaattc
     3121 caaacgatta atttgcaaaa gataaaacac cttgtttata tctttcttct cagactctcc
     3181 tttgttataa tcagtatatt ctgtgattaa atagctgtct tgttgtctca taatcggttc
     3241 aagcttcatt agttgctcat aatgtccccc tgacgaagca caaaaacata ctttttttta
     3301 tttttcattc ctttacttcg ctccatcctt catcaataca actttaactg tcttcaataa
     3361 gatttcaata tctttccaga ttgtccaatc atctatataa gctacatcta acttgacaac
     3421 ttcatcgaaa ttcttaatct cacttcgtcc actaacctgc cataaacctg ttataccagg
     3481 tttaaaactt agacgacgtt tttgttctgg agtatagtgt tcatactcgt ccactgttgg
     3541 tggtcgtgta ccaaccaaac tcatatctcc ctttagaaca ttataaaact gtggcagctc
     3601 gtccaagcta gtcttccgta taaaacgacc aactttcgtg atacgtgggt catcatccac
     3661 cttaaacatt ccaccctgca tggtattttg ttccatgagt tctctttttt tgtcctcagc
     3721 atctacacac atggagcgga acttgtaaaa agtgaagtgg cgaccatttt ttccaatacg
     3781 cgtctgagca aaaatagcag agcccccatc ctttcgaatc aaagggacca gtacaacact
     3841 gactagacca catagtatca gccctaccaa tgcacccacg atatcaataa tccgcttagc
     3901 aatcacgtgg ctagtcttat aaaatgttgt agaaaaaatc acaacgttta atcctgccat
     3961 ctcacgaatt tgcttgttac gtgccaaact acgatcaaaa gcatttagat tgactgttac
     4021 atcaattccc atcgtttcaa actgagagac aagctctcca atattgtatt tttcacttgg
     4081 aagattgata aagacttcat cgaccacctc atgagtcgca aagtttacta tctccccctc
     4141 tgctactacc tttaaacaat catgctgaaa atctggttta tctaagacac tgacggctac
     4201 caactcccca acaacctcat ttgattctat taatctatcc agtacctttt cgacacgaga
     4261 agttgctgta agtaggagaa tcttcttact tcctttaaag ttgggataag cccgcttcca
     4321 ataccacttg ataaataggt ttagcacata gactaagaga gcatgtaatg tgaggaagta
     4381 aatcatgcct cgtctggaaa tactaaatcg atcctctaag aaaaaattag aaatactaat
     4441 cgctagtgca aagaatagga tatatttcaa tgtctggaca agttcaatca aatatcccct
     4501 tttaaagaaa tcctgtccat aatcactgat ataaaagaca aaataatgga ggatataaag
     4561 tgcaatagct gttgctgaag caatctccgc ttctctcaca gcactaagta gataagtcaa
     4621 taaaataaca agaaaactat ggattatggc caatgaagaa tttactactt ttccattcat
     4681 ctctatccct caatctattt tttatttttc ccgtaatctc cataatttcc ataagaaccg
     4741 tatttgttta ctgaagtatc gaatttattc aacacaactc ccaaaaacgg cttccctgtg
     4801 tgttccaact gttcttttgc tttttgaata tcccgtcgat ttatttcacc tgcctccgtc
     4861 actaaaatag aagcatcgca ttttcgcgta ataatagccg catcaatcac gacaccgaca
     4921 ggagcagtat ctacaatgat gtagtcaaaa tatttacgca aggtttcaag cattgtagtg
     4981 aaattcttac tttgaagaag agctgtcgga ttcggtgaca cagagccagc ctgaattaca
     5041 aagagatttt cgatattggt atcacaaagc ccttgtgata ggtctgtagt tcctgataaa
     5101 aattctgtca ggcctgtaat cttatccctt gctttaaaga cacctaacat aacagaattg
     5161 cgaatatctc catcaatcag cagcgttttg taacctgcac gcgcaaaagc ccaagcgata
     5221 ttggtggaag tcgttgattt tccttctcct agtttcacag aagtgataga aaatactttc
     5281 aaaccatctc cacttaactg taggttcatg cacaaagagt tataatattc ctctgccttt
     5341 tttacaaaat ccaattttgc ctgtgagatt tctaatgttg gcataacttc ctcctatttc
     5401 attttgtcca aatctggaac gagccctaaa agtggaattt gcagtacatc ttcaacatct
     5461 tcaggacgtt tcacacgggt gtcgaacaac tcaatcaaaa gaacagcaat tactgttaca
     5521 actgctcctc caagaaaacc aaacaaggaa ttgcgtcgaa catttggaga agaaggagtc
     5581 gtagctggtc gcgcttcttc aagtgtcgtt acatcagata ctcgcgttac agcgacgatc
     5641 ctttctacag caacttctcg tagagaatta gcgatacgac tggcttcctc tggctgttta
     5701 tccttgacag agattgagac gatacgagtg tcagttggta cagtcacttg aactttgcta
     5761 gctaacgttt ttgctggcat gtccaacttc aaatttgtcg ctactttttc caatgcatcc
     5821 tgcgaaagga taatctcacg gtagtctttt accagataag atcctgcctg caaatcctga
     5881 tttgtcagcc ccgacttgtc tccttgattg cgattcacta cgtaaattcg cgtggtactc
     5941 gtatattctg gcttaacaat aaaagtgcta tatgcaaaag cccccgcacc tgtcacaagt
     6001 gccactaata aaatcattag cttgcgtttc cacaaggttt taaataattg aaatacatcg
     6061 atttctatcg tgttttgttc tttcatcatt tctcctaaat tagttgatcc attataattt
     6121 ttcgaggatt gtctataaaa agttcctgag ccttcgcttc tccgtatttt tgggtaacaa
     6181 agtcatatgc ttctgccata tgaggaggtc tgccgtctag attgtgcatg tcacttgcaa
     6241 tgacatgaac caaattctgc tctaaaaaat actgagctct ttttttcatg aatttataac
     6301 gttctccaaa aagttttggt ttgaggacat gtgaactatt tacttgcgtg taacagccca
     6361 tatcaatcag ttctcgaacg cgtttttcat tatttccaag agcatcatag cgctcaatgt
     6421 gggcaatgac tggagtaatt cccaacatca agatcttgct caaggcgcta tgaatatcgc
     6481 gataaggagt gttcatacta aactctatca aggcataacg actatcattg agggtcggaa
     6541 tccgcttttt tcccagctta tccagaacat ctggtgtgta gtaaatttca gccccgtaag
     6601 caatgactaa atcatctgct acttcttttg caatttcccg aacctgaaga aagctttctg
     6661 ctatcttctc ttccggagtt tcaaacatac ccttgcgacg gtgagaggta gagacaatgg
     6721 ttcgcacccc ctgcctgtag gattctgcca agagagcctt gctttcctct cttgacttgg
     6781 gaccgtcatc tacatcaaaa acgatatgcg aatggatgtc tatcatttca tctaccctcc
     6841 atcacatcct gtatagctgc tttaactaca gctaaactac tatcatctat ttccatcata
     6901 tagaggttac tgtctggcat tgcataagaa ggaagatcca tccgacctgt cccttttaaa
     6961 tcttgagaat ttactttata attacctcca ctttccaact gagtattcac taaatctatc
     7021 atagtctcaa tcggcatatt tgtttggata gaatcttgca agctattaat gatcgtacta
     7081 taatttttca gtacttcggt tgacgttaat ttttgaagga tagccacaat caccttttgt
     7141 tggttgcgac cacggtcacg gtctccatcg gctagtgagt agcgttcacg tacaaaacct
     7201 agagcctgct cagagtctag atggacattc cctactggga aatggaactt cccatgtaga
     7261 gctgaaaact cttgatcatt atgaacatct acccctccca ataagtcaat cattttcaag
     7321 aaagaagtga agttcaatag cacatagtaa ttgatatcca ctccatagag gttttctaag
     7381 gtgtgaatgg acgaatcaac tccataaatg cccgcatggg ttaatttatc tttttgatta
     7441 tttccaccat ctgcgattgg tacataggca tcacgtgacg ttgtggtcaa gaggattttc
     7501 ttggtatcac gattgacagt catcaggata ttgacatctg atcgcgacac cgaactaata
     7561 ggtccatagg tgtcaattcc actaacatag atattgaaag actgattctt agacgtctta
     7621 ggagcttcta cttttttagt gaatcccttg gtataaatct tctttatctt cgatgcgtag
     7681 tctggatact ctaactcgat gatattttca aagacactat ttaggacaat ggccttagtc
     7741 tcccctgcaa tcaaactctt gtaagcaacc aagtaagacg aactctggtc gaccgtcaaa
     7801 tcggtattct gacttgactt gatatcagct agtagttttt gaatattttc attatcagtc
     7861 ccagtcggtg ctgtcacact cgtcagttgc gtaacatttt cgatctcact atctgctaaa
     7921 acagcgacac tgagcgaata ttctgagtaa ttagaagtcg catttaaacg attggtcagt
     7981 ccaacaaact gctgtactgc aaagagcgac actgagctga caaggataga gaacaccaac
     8041 agaaaaatag taaatttttc agctttttta tagataatca agagtagccc taccaaggca
     8101 actagtagga ctaacgcagt taccactaga ttaagatatc taaaagcaag gatattgtac
     8161 ttaaagatta agaacaataa aaaacaaact aacaataaat aaatagtcaa caaaactata
     8221 ttaacacttc gcttcacttt ctgtgaacgt gattttttaa aacgtctact catgattaac
     8281 acctatacat tgaacatctt acgattatat cactttttta cggtaatgtc tacacctttt
     8341 tttcactatc tgcatcttta agtatcttag tagacttccc gcaaaacaaa aatatagtaa
     8401 aatgaaataa gaacatgaca aatcgatcgg gacagtcaaa tcgatttcta acaatgtttt
     8461 agaagcagat gtgtactatt ctagtttcaa tctactatag tacacaatga ggtataatct
     8521 tcttatggca tattcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
     8581 tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt atggctggtt
     8641 aaagctaaaa gagaaaacag gagagctaaa ccaccaagta aaaggaacaa aaccaagaaa
     8701 agttgataga gatagactta aaaactatct tactgacaat ccagatgctt atttgactga
     8761 aatagtttct gaatttggct gtcatccaac taccatccac tatgcgctca aagctatggg
     8821 ctacactcga aaaaaaag
//
LOCUS       DBERCA010000066         8671 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.066, whole genome shotgun sequence.
ACCESSION   DBERCA010000066 DBERCA010000000
VERSION     DBERCA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.066"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            2..1681
                     /locus_tag="ACL719_001761"
     CDS             2..1681
                     /locus_tag="ACL719_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGS0699686.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1949..2515
                     /locus_tag="ACL719_001762"
     CDS             1949..2515
                     /locus_tag="ACL719_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699687.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2836..3024
                     /locus_tag="ACL719_001763"
     CDS             2836..3024
                     /locus_tag="ACL719_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGS0699688.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3086..3844
                     /locus_tag="ACL719_001764"
     CDS             3086..3844
                     /locus_tag="ACL719_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS0699689.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3902..4756
                     /locus_tag="ACL719_001765"
     CDS             3902..4756
                     /locus_tag="ACL719_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGS0699690.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4868..5752
                     /locus_tag="ACL719_001766"
     CDS             4868..5752
                     /locus_tag="ACL719_001766"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS0699691.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5915..6994
                     /locus_tag="ACL719_001767"
                     /pseudo
     CDS             5915..6994
                     /locus_tag="ACL719_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            6988..8324
                     /locus_tag="ACL719_001768"
                     /pseudo
     CDS             6988..8324
                     /locus_tag="ACL719_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
     gene            complement(8365..>8671)
                     /locus_tag="ACL719_001769"
     CDS             complement(8365..>8671)
                     /locus_tag="ACL719_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HGS0699692.1"
                     /translation="NKTNKRIVTQLAINCFILSIDKEEFSNCSYLINEIKGLLENELN
                     FYEQTVFLYATGYYEFKRQLSSGIETMKQAIQVLDILGEDKLKLHYTSHFDKLVNNK"
BASE COUNT         3077 a         1208 c         1783 g         2603 t
ORIGIN      
        1 tatgtcaaaa gaaaaaataa aagtatacct ctatacacga gtatctacgt caatacagat
       61 agaggggtat tcgttagagg cacaaaaatc acgaatgaaa gcttttgcta tttacaatga
      121 ttatgaaatt gttggagaat atgaagatgc cgggaagtct ggcaagtcta ttgaaggtag
      181 aatacagttc aatcgaatga tggaagatat aaaatctgga aaagatggag tatcttttgt
      241 tcttgtgttt aagttatcaa gatttgcaag aaatgccgct gatgttcttt ctacacttca
      301 gataatgcag gattatggag ttaatcttat ctgcgttgaa gatggcattg attcatctaa
      361 agatgctggg aaactaatga tttctgtttt atcagctgtg gctgaaatag aaagagaaaa
      421 catccgtatt caaacaatgg aaggtcgcat tcaaaaagca agggaaggaa aatggaatgg
      481 tggctttgct ccgtatggat ataaacttga agacggcaag ctgtttataa atgaggaaga
      541 ggcagttgca ataagaacga ttttcgacca gtatgtaaat actacgatag gagccaatgg
      601 gatatctaaa tacttagaga atcatggaat tagaaaaatc ccaagacaga atggtaagaa
      661 tcctttgttt gatgcaggtc ttataagaaa gatattaaag aatcctgtat ataatgggaa
      721 aatagcattt ggaagaagaa ctttagaaaa agttcatggt acaagaaatg aatataagca
      781 ggttgaacaa gatgaatatc taatatctga agggatacat gaagctatag tttccgatga
      841 agtttggcaa gctgctcagg ttaagctaaa atctcaagca aagaaatatg agcatgtgaa
      901 taaaggaaaa gatacacgca cacacttgct ttctggaatt gtaaaatgcc cgatatgtgg
      961 agtgggaatg tttgggaaca agtgtatcaa gaaaaagaaa gatggcacaa agtataaaga
     1021 tttttattac tatggttata aacataggca gatgataaga ggtcataagt gtacattcag
     1081 taagcaaatt agagaagaat tgttagatga tgctgttgca gaggtgattg tcaagatagt
     1141 aagtaatccg aaatttgctt ctatgatgca agaaaaaatc aacatgaagg tggatacctc
     1201 tgaaatagaa aaagaaatag ataattacca aaaggaattg agaaagagtc attctacaaa
     1261 gtttaagcta attgaggaaa tagataattt agatgttgaa gataagcatt ataagagaag
     1321 gaaacaggat ttagatgata gactatatcg tatgtatgac aaaatagatg aattagaatc
     1381 atcactaatt gatgcgaaag caaagaaaca gactattgaa gctgaaaagc ttacaggaga
     1441 taacatatat aaggttctga tctattttga taagctctat aaagtcatga atgatgtaga
     1501 gcgtaggcag ttaatttcag ctttgatttc tgaaattcaa gtttatgaag aaaaacaatc
     1561 taacggacaa tggctaaaat ccattacttt taagctacca attatagaag aaaatctcaa
     1621 cataggtttg gataatgatg aacaagttga atgtgtaagt ttattagaga aacgtagtta
     1681 atcctcaaaa ggttccccaa accttttgag tcccactgac gcatcacgct gaggcggtat
     1741 cactgcttgt acgagttgaa gcatcagcga agtaaaagta gatgttcagc caatgcgacg
     1801 aggtgagacc gaaggtcgat aaggtgtgtt ctgggtatag gactcgtaaa gtaagtagga
     1861 aatatgatga gatgatacag agagcatgaa ttggtcaggt gacgtgctta tttacattag
     1921 cctgataatg aaaatggaga gattatcgat ggttatattg gaattgtacc aaaatgatta
     1981 ttctaaagat ttagtagcat ttgattccat agaagatggg aaagcctttg tagctcaaat
     2041 cccgggatac actctggaaa cagaagatgg ttttgaagtg gagtatttta atcctaaaaa
     2101 cataccggat tatatggaaa ttatttttaa cggaaatatt gttcctttat ctaaatttat
     2161 gttcgatcct gaggaaaatg taaacattat ttggaaagag atttcaaatt tatccctcaa
     2221 aaatgataga gtgatcgaag gatattcaaa aattgatgca tatgtcgtta acaaccatga
     2281 agttaaagcc tatgtcgaaa caagagaaac gaactatcgt aaagcaaaag acttcttaga
     2341 aagccgcgga tatgaaatcg acagaagctt tttcggaagt gaagacggtg aagcgattct
     2401 ttacagaaaa aaagggatcg aagtttggca tttcttgtgt cacttagacc caatgtttgt
     2461 agagattgaa gatgtagaag gatatgttaa agaagaagtt ggagagattc aatagtcaac
     2521 caaagttcct tgtgagtata tacctttgct agtaaaagag tcaaaacgtt ataacgaagg
     2581 ggggaagaaa acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag
     2641 ccgctcataa tttatactag atttgataac tgatttgaga cagattttat ttctataaat
     2701 attgtgaaaa ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact
     2761 tttaaactaa tgtattataa aagccagttg acacaagtgt tacacctatg atagaattaa
     2821 cttgaggtga tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa
     2881 aatctagaga aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta
     2941 acaaagtccc aggttattga attactccta aataccgttg attatttcga tgatattatg
     3001 ggggctattt actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta
     3061 acactagtga tataggagtg atgaaatggt tgtttatatt agacaaagta aattaccaag
     3121 tgaggtttca attaataaat acaatgctca agtaggtgcc tacttacaag gagaagaagt
     3181 tattttatat caatcttttt ctgaaataaa agagttaaca agtgaagata tagttgtaga
     3241 ttatataatg gagactagag cattactaaa aatgatgggc ttaaacgttc cggttcatga
     3301 ctatcctatt gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga
     3361 gattgtgaat atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt
     3421 ctttactgga agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga
     3481 ctatcctata tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt
     3541 agatggtcgc gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc
     3601 aagtgtaatt gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga
     3661 tatcggtgtt actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt
     3721 gggaaattat ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga
     3781 aatggtaaaa ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttatttt
     3841 ctaaaataaa tatggtagat aaccaatata aaaagcatca aaatgaacaa ggaggaatct
     3901 gatgcttgga gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa
     3961 aacaaaggac tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac
     4021 ctgtgctgtt gcagaagcaa ttatgaatgg tggacaaaaa gacgacttca ttgacgcgat
     4081 gaagaaatat ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcatggct
     4141 aaacagcgat aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc
     4201 tccatgtgct tggatcatgg actgtggttt ttatgcgaaa actggtatat ggccatcatc
     4261 tagaggactt acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg
     4321 cgctatggct acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag
     4381 agagtacgag caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta
     4441 tatagaaaaa gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta
     4501 tcgttttaac gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag
     4561 tagagacttc gaagatgcga taagaaatgc catctcactt ggtggcgaca gtgatactct
     4621 cgcagcaatc acttgtagca tagcagaggc agcttacggt attcctgatt ggatcaagga
     4681 taaggcctat tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag
     4741 aataaaagcg tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata
     4801 ataggattac atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat
     4861 aaaatcaatg aaaaaagcaa tggtaattat caaccctact tctggtggcg agaaggcttt
     4921 ggattacaaa gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa
     4981 aattaccgaa aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta
     5041 tgatgcagtg gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga
     5101 tgagagagac tacattccta agttagggat tatcccaggc ggtacgggta acctcattac
     5161 aaaacttttg gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt
     5221 aaccaataag attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat
     5281 cggttctctg cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat
     5341 tttaacctat gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa
     5401 ggttgagaca gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac
     5461 aaattacttc gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt
     5521 gattctgaaa gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg
     5581 ggatgttgtc gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc
     5641 agatagtgaa ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat
     5701 caaagtccta gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga
     5761 gaaagccttt tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat
     5821 tccttgcaaa tagtttacaa aaatagtata ctggatttat tgagtttgaa aacgtttgcg
     5881 taaaatttga atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag
     5941 gcctgagtcg gagtatgcct atctttataa ggataagaaa ctccatattc gaattcgaac
     6001 taagaaaggg gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga
     6061 ggagttttat caggatacaa aagaaatggt caagataact tctggtacct tatttgacca
     6121 ttggcaggtt gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga
     6181 tacagaaggt caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa
     6241 tcttcatgca attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa
     6301 ggttgactgg gtttcaaata cggtatggta tcagatattt cctgaaagat ttgccaatgg
     6361 caatgctcta ttaaacccag aagggacttt agactgggat tcatctgtca cacctaagag
     6421 cgatgatttc tttggtggtg atttacaggg gattattgat catatggatt acttgcaaga
     6481 cttgggtatt actggactat atctttgtcc catctttgaa tctacaagca atcacaagta
     6541 caatacgaca gattactttg aaattgaccg tcattttgga gacaaggaga cctttcggga
     6601 actggtggat caagcgcatc atcgtggcat gaaagtcatg ctggatgcgg tatttaatca
     6661 tattggttcg caatctcttc aatggaaaaa tgtcgtcaaa aatggtgaac agtctgctta
     6721 taaggattgg ttccatattc aacaattccc agtgacaact gaaaagctag ttaataagag
     6781 agacttaccc tatcatgttt ttggtttcga ggactatatg cctaagctaa atacagccaa
     6841 tccagaggtc aagaattatc ttttaaaggt tgcgacttat tggattgaag agtttaatat
     6901 cgatgcttgg cgtttggatg tggctaatga gattgaccat cagttctgga aggattttcg
     6961 taaggcagtt ttagctaaaa atcctgatct tttcgagatt ataaaaaata taaagaatct
     7021 agtcataaat ttgaattgaa acaacgtctt tattttttgc ttcagcaatc taaatcgttt
     7081 gatgattttt tagagaaagc aaagcagttg catgttcaga ttgattttag tcaaaagcat
     7141 agccgttttt tgatgacaga tagaacgatg ataaaaccaa ttcgagggcg ccaactcagc
     7201 aaacgagatc tatatgatga agaatttttt agaacgcatt ttgctaagca agagattgaa
     7261 agtcgtttag aatttttgtt aaaccgtgtc aattctttag aagatttaat aacaaaagca
     7321 aaagaattga atctaaccat tgacttaaaa caaaaaatgt aacttttatc ctggaagaaa
     7381 ataatcaaaa gataagtttg ggtcatcaaa aaataagtga taagaaatta tatgatgtca
     7441 aattttttca agattatttt aaaaataagg aagtcgttgc ttcagaagga ttagagaatt
     7501 tacaggaaca gtaccatgct ttccaagaag aacgagataa ggataaagta tccactgaag
     7561 agattgagaa agcctttaag acatttaagg aaaaatgaga taccgttcgt gaatttgaag
     7621 tggaacttgc agagaaccaa atagagaagc tagttgataa gggcgtctat atcaaggtgt
     7681 ctttcggtgt caaacagagc ggtcttattt tcattcccaa ctatcaattg gatattatgg
     7741 aagaagaaaa tcataaaaaa tataaagtct acattcgaga aacgtcttct tactttgtct
     7801 ataataaaga aaacatggat aataattgtt tcatcaaggg taggacctta atcagacaat
     7861 tgagcaacga tagccaaaaa ctcccctata gacgaccaac tttaaaaagt cttcaagaga
     7921 agatttctga aattaatctt atgattgaac tatctaacac gaacaaacaa taccaagaaa
     7981 tcaaagatga gcatgtatta gaaattgcag agattgatat gaagctagaa gaaacccaag
     8041 aaaaaatcgc caccttaaac aagatggcgg aagtccttat caatttgaag agtgaagatc
     8101 atgagactag aaaattagct aagtatgatt ttgaccaaat gaacatgacg gaatcaataa
     8161 tgttagatcg actcaataca gatattttaa aattacaaca ggaacttggt aatgaaatta
     8221 ataaatatga ggagatagct agaagattgg atttgtttgt gaaaattatt aataccaata
     8281 aatttacagt tctaaaattt cacgagaatg ccttactaga ataatattat ttacgactaa
     8341 ttagttaaaa gatagagaat catttcattt attgtttaca agtttatcaa aatggctagt
     8401 atagtgtaat tttaacttgt cttctcctaa aatatcgaga acttgtatag cttgtttcat
     8461 tgtttcaata ccactactta attgacgttt aaattcatag taaccggttg catagagaaa
     8521 aactgtctgt tcataaaagt ttaattcatt ctctaacaat ccctttatct cattaattaa
     8581 ataagagcaa ttagaaaatt cttctttatc tatgctgaga atgaaacaat ttatagctag
     8641 ctgagtgaca atccgcttat ttgttttatt t
//
LOCUS       DBERCA010000067         8455 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.067, whole genome shotgun sequence.
ACCESSION   DBERCA010000067 DBERCA010000000
VERSION     DBERCA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.067"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..332
                     /locus_tag="ACL719_001770"
     CDS             <1..332
                     /locus_tag="ACL719_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HGS0699693.1"
                     /translation="IQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVV
                     IQKQNEIEELSTNIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMY
                     RKYGNIN"
     gene            345..1820
                     /gene="galT"
                     /locus_tag="ACL719_001771"
     CDS             345..1820
                     /gene="galT"
                     /locus_tag="ACL719_001771"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS0699694.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1833..2813
                     /gene="galE"
                     /locus_tag="ACL719_001772"
     CDS             1833..2813
                     /gene="galE"
                     /locus_tag="ACL719_001772"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS0699695.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            3023..3682
                     /locus_tag="ACL719_001773"
     CDS             3023..3682
                     /locus_tag="ACL719_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGS0699696.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3707..4774
                     /locus_tag="ACL719_001774"
     CDS             3707..4774
                     /locus_tag="ACL719_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0699697.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4877..5887
                     /locus_tag="ACL719_001775"
     CDS             4877..5887
                     /locus_tag="ACL719_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699698.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5899..7590
                     /locus_tag="ACL719_001776"
     CDS             5899..7590
                     /locus_tag="ACL719_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699699.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7593..8303
                     /locus_tag="ACL719_001777"
     CDS             7593..8303
                     /locus_tag="ACL719_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGS0699700.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2885 a         1162 c         1654 g         2754 t
ORIGIN      
        1 ttattcaaaa taatgaaatt ttgaagaaaa ttatttcaga aataaaaata cttcatgaag
       61 ttatcggact gcatatgaat agggctatat cttgttatag agaggaacaa tctggatgtt
      121 tagatatggt tgttatacaa aaacagaatg agatagaaga gcttagtaca aatattgaaa
      181 agaaaataat gaattacata tttgaagatg atgggaatgt ttctgaagta attggtgctt
      241 tagatattat tcatcacctt gataaaattg ctcatactac gcaatcaatt tataaatgga
      301 taatgtatag aaaatatggg aacataaatt agaaagaaaa atatatgaaa acaattattg
      361 ataattttgt tgatagagta attgaatacg gaatgtataa tgaaattgac aaaatatatg
      421 tgaaaaatag agtactagcg ttaataggtg aggagggaac tgatcgcatt tccgatgaaa
      481 atgacctgaa acaaataaaa gattacttag tggaaatcgc cttaaaaaac ggtaaaatta
      541 aagatttaat cgaggaaaag gaatgtttag gagcagagtt aatgaatttc atagttcctt
      601 tacctagtcg attaaatgat attttttgga gttcatatga tatatcacct caagaagcag
      661 ttgaagagtt ttataagtta agtaaagata gtgattatat taaaacttct gctattgcta
      721 agaatattga gtttcgagca tcaactaaat acggagagct agagattacg ataaatttgt
      781 caaaacccga aaaagatcca aaaacaattg ctgctgaaaa attagtaaaa gccactaatt
      841 atcccaaatg tttactatgt atggaaaatg aaggatatca ggggcgaata aattatccag
      901 cacgttcgaa tcatagaatt attcgattga aacttggtga cgaagtatgg ggattccaat
      961 attctcccta cttatacttt aatgagcatg cgatattttt aaatagtcaa catgtaccaa
     1021 tggctattac ctctaaaaca tttgaacaat tattggagat tgttgatatt ttgccaggat
     1081 attttgctgg ttcaaattct gatcttccta tctcaggagg ctccattctt agtcataacc
     1141 actaccaggg agggaaacat attttcccaa tggaaaaagc aaaatttgag agtgaatttc
     1201 gttttaaaga ctttgaggat gttaatgctg gtattgtaaa atggccaatg tcagtaatta
     1261 ggttacaaag tgaaaataaa aatcgcttgt tagatttagc tactaaaatt ctaaataagt
     1321 ggagagaata ttcagattta gaagtagaca tcattgctat gactgaagat gtcccacacc
     1381 atacggtgac tcctattgct cgtaaagtgg acgggagata tgaattagat attgttctaa
     1441 gagataatca tactactgaa cagtacccag atggtgtttt ccatcctcat caagatgtac
     1501 aacatattaa aaaggaaaat atcgggttaa tcgaagtgat gggacttgcg atattaccac
     1561 ctcgtttgaa accagagtta gaagaagttg gaaaatatct tttaggagag gataacgcaa
     1621 ttgcggatta tcatttagaa tgggccgacc aattgaaaga aaaatatcct cgcattaata
     1681 aagaagaggt taatagcgta gttcaacacg aagcaggaca agtgtttgcg agagttctgg
     1741 aagatgcagg agtttataag aatacaccgt ctgggcatga agcgtttatg agatttgtta
     1801 agtcggttgg aattaattag gaggaaacaa aaatggcaat attggtaaca ggcggagctg
     1861 gttatattgg tagccatacc gtagtagaat tactaaattt aggaaaggaa gtcatcattg
     1921 tcgacaacct ttcgaactct agcatcttgg tgctagaccg tattgaagca attacaggaa
     1981 tacgtcccgt gttttacgaa ttagatgttt gtgataaacc agcattgaga aaggtatttg
     2041 aacaagaatc gattgatgct gcaattcatt ttgcaggtta taaagctgtc ggagaatccg
     2101 tgcaaaagcc tgtgatgtac tacaaaaata atattatgag tacattggca cttgttgaag
     2161 tgatgtcaga atttaatgtt aaaaagattg tcttttcttc aagtgcgact gtatatggaa
     2221 ttaacaatca gtcacctcta attgagacta tgcaaacaag tgcgacaaat ccttatgggt
     2281 atacgaaagt gatgcttgag caaattttaa aagatgttca tgtggcagat tcagaatgga
     2341 gtattgcgtt gcttcgttat ttcaatccaa ttggtgctca tgagtctggt ttgattgggg
     2401 aagatccctc aggaattcct aacaacttga tgccctatat tgcacaagta gcggtaggta
     2461 agctgtcaga actaagtgtg ttcggaaatg attatgatac gcttgacggt actggtgtgc
     2521 gcgattatat ccatgtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat
     2581 ctgaaaaaac agatgtttat atctataacc taggctcggg agaaggcact agtgtattac
     2641 aacttgtaaa tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa
     2701 gacgttcagg agacgttgca acttgttatg caaatgcaga caaagcatat aaggaattaa
     2761 attggaggac aacaaaatcg attgaagaca tgtgtagaga tacatggaat tagcaatcaa
     2821 aaaatcccaa tggctataat tgatgatact gtctatatga gtgctgtatt ttggaaaaat
     2881 tacttaacct tttaatgaaa tgcatattta atgaaagata aattaaaaaa aatgtttgac
     2941 agcgcttgac aaaggaaacg gtttcattgt ataatgtaaa catagtgaca ccgatgtcaa
     3001 atataaaaaa gaaggtcttt atatgaaaaa cttaacaaaa attaagttca aagagaatgg
     3061 ggaatttaat catttccctg ggaatacagt tgtagcaaat ctttatacta aacaagattt
     3121 gatggaagtt gttgatatta ttcaatcacg ttatagagaa ttaccattta tcgataagtt
     3181 tactttaact ccaaggaatt ctattcacat gactgtaatt gaattgttgt gccatgaaaa
     3241 tcgtgaaacg gagttttgga gcagtaatct tcctctagat acacctttac aggaaataca
     3301 tgattacttt gctaaacaac ttgaaatttt tccattgttg gatgaagaaa ttcatatgcg
     3361 tataactgaa atgggaaaac aaaacatact agttgaacct gcagatgaag cttctgcaaa
     3421 gagattagaa gaaattcgta cttatgtctc agaaaaagca ggtgtttgtt tccctaatca
     3481 tgatagatat caattccata tttcaattgg gtatcttcgg attcctctaa ccgaagagga
     3541 ggaagaagag tttactaaag tcagagcaga attaactgaa attttattag agaagattcc
     3601 aactattact gtaaaccgta ttgactatac tgtatttgaa gatatgagac aatttgttcc
     3661 atatcacgaa aaatttaaat aaaataataa aaaaggagaa cttatcatga aaaaaatgaa
     3721 agtttggtct actgtacttg caacgggagt tgctcttact acacttgctg cttgctctgg
     3781 aggttcaaat tctacgactg cttcttcatc tgaagaaaaa gctgataaaa gtcaagaatt
     3841 agtaatctat tcgaactcag tctcaaatgg tcgtggtgat tggttaactg ctaaagcaaa
     3901 agaagctggt tttaatataa aaatggttga tattgctggc gctcaattag cagaccgtgt
     3961 tattgctgag aagaataatg cagttgcaga tatggtattt ggaattggtg ctgttgactc
     4021 aaataaaatt agagatcaaa aattactagt acagtacaag cctaaatggt tagataaaat
     4081 tgatcaatct ttatcagata aagataatta ttataatcct gtgattgttc aaccattagt
     4141 tttaattggg gcgcctgatg taaaagaaat gcctaaagat tggactgaat taggtagtaa
     4201 gtataaaggt aaatattcaa tttatggtct tcaaggtggt acaggacggg caattctagc
     4261 aagtatctta gttcgttacc ttgatgaaaa aggagattta ggcgtatcag aaaaaggatg
     4321 ggaagttgca aaagaatact ttgcaaatgc ctatactctt caaaaaggtg aaagctcagt
     4381 tgttaaagcg ttagataaag aaagcccaat tcaatacggt atgatctggg gttctggagc
     4441 gttaatcgga caaaaagaac aaaatgttga attcaaagtt atgactcctg agattggtgt
     4501 accatttgta actgaacaaa ctatggtttt aagcactagt aaaaaacaag cgttagctaa
     4561 agaatttatt gattggtttg gtcaatcaga aattcaagta gaatatagta aaaaatttgg
     4621 atctattcct gcaaataaag atgccctaaa agagttacct gaagatacga aaaaatttgt
     4681 tgatcaagtg aaaccacaaa atattgactg ggaagctgtt ggaaagcatt tggatgaatg
     4741 ggtagaaaaa gctgaattag aatacgtaaa ataagaaata agagttattt tttatgtcca
     4801 agtctctcta ttatttctaa atttaaggaa gaaataaaga gaggcttttg gtttagtaat
     4861 agagggagtt agtgagatga ttaaatttga taatattcaa attaaatatg gtgattttgt
     4921 tgcaattgat aatctgaatt tagatataca tgaaggggaa ttctttacat ttcttgggcc
     4981 ttcaggatgt ggtaaatcaa ctactttgag agcattggta ggttttttag atccatcatc
     5041 aggaagtatt gaagttaatg gaacagatgt cactcatttg gaacctgaaa agcgtggaat
     5101 tggtattgta tttcaatctt atgcgctatt tccaactatg actgtttttg ataatattgc
     5161 atttggttta aaagttaaga aggtagctcc agatgttatt aaagctaaag tatcagcagt
     5221 ggcagcaaaa attaagatct ctgatcaaca gttacagcgt aatgtatcag aattatctgg
     5281 gggtcaacaa caaagggtag cattggctcg tgctctggtt cttgaaccta aaattctttg
     5341 tctagatgaa ccattgtcaa accttgacgc aaaattacgt gtagatttga gaaaagagtt
     5401 gaaaagactt caaaaagagt taggtattac tactttatat gttactcatg atcaagagga
     5461 agccttgact ttatctgata gaattgcagt ctttaacaat ggatacatcg aacaggtcgg
     5521 tacaccagta gagatttatc ataattctca aactgaattt gtatgtgatt ttattggaga
     5581 tattaatgtt ttgaccgatg aaacagtcca cgaagtatta ctgaaaaata caagcgtttt
     5641 cttagaagat aaaaaaggat acattcgatt agagaaagtt cgattcaatc gtgaaactga
     5701 acaagatttc attctaaaag ggacaattat tgatgttgag ttttctggag ttacaattca
     5761 ctatacaata aaagtttctg aaagtcagat tcttaatgta acaagtattg atagtcaggc
     5821 tgctattaga tctgtcggag aaagtgtgga attatttatc acaccatcag acgttctgca
     5881 attttaagga ggtgcagtat gcgtcataaa ttaaatttaa aagattggct tattcgttta
     5941 gggttaatct ggttcttagt aacatttatt atttatccaa actttgatct agtagtgaat
     6001 gtatttgtaa aaggaggaga attttccctt gatgctgtac atcgtgttct aaaatctcag
     6061 agggcacttc agagtattat gaacagtttt aagttagcat tttcactcat tattacagtt
     6121 aatgtcgtag gtattctttg tgttctattt acagagtact ttgatattaa aggtgctaaa
     6181 attttaaaat taggttatat gacctcttta atttatggag gagtggtttt agcgactgga
     6241 tataaatttg tctatggtcc atatggattg attacaaaat ttttacaaaa tgttatccct
     6301 tctttagacc ctaactggtt tattgggtat ggtgcagtct tattcattat gacattttca
     6361 ggaactgcta atcatacatt gtttttaaca aatacaattc gaagcgttga ctatcacact
     6421 attgaggctg ctcgaaatat gggagcaaaa ccatttactg ttttccgaaa agtagtgtta
     6481 ccaaccttaa ttccaactct atttgcactt actattatgg tttttcttag tggtttatct
     6541 gcagtagcag cacccatgat tgttggtggt aaagaatttc aaactataaa tccaatgatt
     6601 attacatttg cagggatggg gaattctcgt gatttagctg ccttacttgc aattatttta
     6661 ggtattgcaa ctacaatttt gcttactatc atgaataaga tagaaaaagg tggaaattat
     6721 atttctatct ctaagactaa agcgcctctt aaaaaacaaa aaattgcgtc taagccttgg
     6781 aatatcattg ctcacattgt agcatatgga ttgttcacag ttttcatgct tccactaatt
     6841 tttatagtat tatactcatt tacagatcca gttgcaattc aaacaggtaa cttaacatta
     6901 tcaaacttta ctttagaaaa ttatcgctta ttctttagta atagtgcggc attctctcca
     6961 ttcttggtca gctttattta ttctattatt gctgcgacaa cagcaacaat tctcgcagtt
     7021 gtatttgctc gtgttgtcag aaaacataaa tctcgttttg atttcttatt tgaatatggt
     7081 gctctacttc cttggttatt accaagtacg cttttagcag taagtttatt atttactttt
     7141 aatcagccac aatttcttgt cttgaatcag attttggtag gtagtttggt aattctactt
     7201 attgcatata tagttgtaaa aatcccattt tcttatagaa tggtacgtgc tattttattt
     7261 agtgttgatg atgagatgga agatgcagca agaagtatgg gtgcttcacc tttttatact
     7321 atgatgaagg ttatcattcc atttatttta ccggttgttc tctctgttat tgctttaaac
     7381 tttaactctt tattaactga cttcgactta tctgtattcc tttaccatcc cctagctcaa
     7441 ccattaggta ttacgattcg atctgcaggt gatgaaacag caacatctaa tgcacaagct
     7501 ctggtatttg tttatacaat tgttctgatg attatttctg gaacggtatt atacttcaca
     7561 caaagaccgg ggcgtaaagt aaggaaataa tcatgacagc cactagtctt gggttatcaa
     7621 atattgaaat agttgtcagg attgttttat cagtagtcat tggtagtata attggtttag
     7681 agagagggag caaatcccag cctgcaggca tccgaactta tagtattgtt tgtctagctg
     7741 catgtttgat tatgatgacg aatgaatacg tatcttataa atttgggaca ggagatccta
     7801 cacgattagg agctcaagtt atatcaggtg tgggttttct aggcgctgga acgattctta
     7861 ttacagataa aaagaaaatt acaggtctga caactgcagc aggcatttgg gcttcggcag
     7921 gaattggatt agctattgga gtaggttttt atgagggagc tcttttagta gccatttctg
     7981 tttggggtgt gatatccatg ttccaaccac taaaaaaata tctacaaaat cgttctaaaa
     8041 tgattgaatt gtatatagtg gttaaatcta cagaagccta taatcgtgta ttggtttatt
     8101 gtgcagaaaa tggtatcaga atgaccgatt caagaacagc gtttggagat gttaattctg
     8161 acagaattga atactttgat gttccagata aaaaaatagc gtcatttatt actctggaat
     8221 tatcaggtag atttgaacat cttcgactga tggaagaaat tgctaatatt gtaggtgtta
     8281 tttatgtcga agaaattagc tgaaatttag taaacaaagg gacagatgtt tttgtccttt
     8341 tgttttttag atgttttaat catctaattt ttgagtagat ttttgctcag aggtttttat
     8401 gaaagattat aaaatcaatt ttgatttagg taaaatagaa tattttgata ataat
//
LOCUS       DBERCA010000068         7683 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.068, whole genome shotgun sequence.
ACCESSION   DBERCA010000068 DBERCA010000000
VERSION     DBERCA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.068"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            65..607
                     /locus_tag="ACL719_001778"
     CDS             65..607
                     /locus_tag="ACL719_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS0699701.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(648..721)
                     /locus_tag="ACL719_001779"
     tRNA            complement(648..721)
                     /locus_tag="ACL719_001779"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(685..687),aa:Asn,seq:gtt)
     gene            complement(727..798)
                     /locus_tag="ACL719_001780"
     tRNA            complement(727..798)
                     /locus_tag="ACL719_001780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Glu,seq:ttc)
     gene            complement(850..1602)
                     /gene="comE"
                     /locus_tag="ACL719_001781"
     CDS             complement(850..1602)
                     /gene="comE"
                     /locus_tag="ACL719_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGS0699702.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1599..2924)
                     /gene="comD"
                     /locus_tag="ACL719_001782"
     CDS             complement(1599..2924)
                     /gene="comD"
                     /locus_tag="ACL719_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGS0699703.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2945..3070)
                     /gene="comC"
                     /locus_tag="ACL719_001783"
     CDS             complement(2945..3070)
                     /gene="comC"
                     /locus_tag="ACL719_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGS0699704.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(3235..3308)
                     /locus_tag="ACL719_001784"
     tRNA            complement(3235..3308)
                     /locus_tag="ACL719_001784"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3272..3274),aa:Arg,seq:tct)
     gene            complement(3352..3831)
                     /gene="rlmH"
                     /locus_tag="ACL719_001785"
     CDS             complement(3352..3831)
                     /gene="rlmH"
                     /locus_tag="ACL719_001785"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGS0699705.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4014..5195
                     /locus_tag="ACL719_001786"
     CDS             4014..5195
                     /locus_tag="ACL719_001786"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGS0699706.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5253..6011
                     /locus_tag="ACL719_001787"
     CDS             5253..6011
                     /locus_tag="ACL719_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGS0699707.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEALAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6224..7585
                     /gene="dnaA"
                     /locus_tag="ACL719_001788"
     CDS             6224..7585
                     /gene="dnaA"
                     /locus_tag="ACL719_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGS0699708.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2674 a         1383 c         1211 g         2415 t
ORIGIN      
        1 ctaaattaca agataaaaaa tctattttta gacatatagt ctaatttgtt tacaaggagg
       61 aaatatgcaa gaaagtaaca aacgcttaaa aacaaagcga actattgaaa atgctatggt
      121 acaattactg atggaacagc catttgataa aatttctact gtcaaattag tagaaaaagc
      181 cggaattagt cgttccagct tctatactca ctataaggat aagtatgata tgattgagca
      241 ctatcaaagc aagctattcc atacatttga atatattttt caaaaacatg ctcatcacaa
      301 aagagacgct attttagaag tttttgaata tctagagtca gaaccacttc tggctgccct
      361 tctttctgaa aatgggacta aagaaatcca aaatttctta cgaaataaac ttcatatcat
      421 gcttagtaca gatttacaaa agcgatttat gcaactgaat ctaaatacca ctgaattaga
      481 atatagtagc atctatctaa ctcatgcact ttttggtgtc tgccaaactt ggattgcaca
      541 tggaaaaaaa gaaagttctc aagaaataac agacttcctt atgaaaatgc ttggtgatac
      601 aaattgatac aaaaagagga acacagctgt gttccctttt atctatactc cgccagtagg
      661 actcgaacct acgacatcat gattaacagt catgcgctac taccaactga gctatggcgg
      721 ataaaatagt ccgtacggga ttcgaacccg tgttaccgcc gtgaaaaggc ggtgtcttaa
      781 ccccttgacc aacggacctt ctatctgtag cagatataac cattatatca atttcttgct
      841 aattgtcaat cacttttgag attttttctc taaaatatct tttaattttc taatttttaa
      901 tcttgaaata ggacaacgat ggtcttcata gaaaacaatt tctaagtttt ttcgatcaat
      961 ttctctgata ttgcctatat ttaccaaaaa tgacttgtga ggagaataaa atcgctgagt
     1021 atgtttgtcc ttttcctgaa tatctgtcat ggtaccataa aactcttttg caaaattctt
     1081 accaataatg cgcaatttat gagatacccc tgttgtttca atatacaaaa tatcatggta
     1141 aggaattttt aaatcatttc ccttgtaatt gtagtcgaaa taatctacaa catcttcatt
     1201 ttcaagtaac atactcttcg tgtagaagat attttgctca attctcttct taaacatctc
     1261 atcattgata tccttatcaa caaaatctag ggctgatacc tggtatttat aggttagagt
     1321 cgcaaactct gatcgactag tgataaagac gataatagcg taaggattgt aatgacgaat
     1381 gagctgagcc acttcaaatc cctttttctc aattccatga atatcgatat ctaggaaata
     1441 aagctgattt acttcatcat tttcaatgta ttcttcaaat tcacggactt ttcccgttgt
     1501 cttgtatgat attggaatat tcgattcttt cgaaatttca tccaatattc tctctagtct
     1561 cacttgatgt tcaataacat cttctaaaat taaaactttc attcaaattc cctcttaaat
     1621 ctaatgattt gtctaaatgt actgccttcc atctctgttt ctaaaataat attgttgtac
     1681 ttatctagta gttctttcac attatttaat ccgactccgc gatttcttcc cttagtggag
     1741 aatcctaagg caaatagatc tcctgaagga gtcatcgtca ttttacatga attctgaatc
     1801 acaataactg tttcagtttc catcttaata actgctactt ccatctgctt tttatagcta
     1861 tcagccgatc cttcgacagc attattcaat aaaacgctca tgatacgaac caaatccaat
     1921 agttcaattg gaagcttggt aatcgtatct tttacttcca gtgtaaactc tacaccatta
     1981 tttcgagcat agacaattga ctgagcaacc aaacttcgta aagctgagtc ttctatgttg
     2041 ttcaaatcaa agtaagtgta cttatctgaa cgcaatttat gatttgcttt gactaaaact
     2101 tcattgtaaa ttctgtcaat ttcctgtaaa ttaccactgt caattgccat ctgcatgctg
     2161 acaagcattc cagcataatc atgtcgaaaa ccacggattt cattatacag accaacaatt
     2221 tcatctgtgt aattctgtaa atgtttctgt tcaaatttct tctgcttcaa agcaatctct
     2281 ttctccattt gaactttatg agaattcatt gcaaagaagg tcaaaaggag agagataaag
     2341 acaatagatg acaaaatact tccaaaacta ttcaaatgtt taatcgtact taccatatct
     2401 gaaacgaaag atacaatatg tagcaatagt aaagcaaaaa atactttttt caagaaagga
     2461 taaaggtagt cctcgtcaaa ataggctagt tccaaatgga aatagtaaat gatttttaat
     2521 gtaacaaaat aggttaacac cgtcacaacg aaaaagaatg ggaaatgata ttgtaaaaca
     2581 aaattatctc ctgttataga ggagaaagtt acggacagaa agttatgagt gctctcatat
     2641 aaaagagata gtagtaaact taggaatagt cctctatccc tctcatactg tttcatccat
     2701 cgaaaatagg aatataatcc caaaggaaat aaaaatcttt caatccctaa cccatctaaa
     2761 tatagaatat ataagggaaa atcaaatact atttctgtta gtaatgtata agcaccaaaa
     2821 acgaataatt cttttctatt tatttgacct ttacaaataa aatggtaact gtgactaata
     2881 attaaaaaat gaacaataac cgtcccaaat ccaaataaat ccattactct ttccccttat
     2941 ttcattactt ttttctttgt aaaataaaat cacggaagaa ttttgacaac ctcatctccc
     3001 cacctttaat cttttgtaag tctttttcct tcaaagctac aaactgttcc aatttaactg
     3061 tgtttttcat aataaaatct cctaaaatgt tttttcttgt aagctaactt acaaaaacca
     3121 ttatacaaaa tggaatttcg ttttagataa aattctctca actgtcattt ttttctccca
     3181 aagtgtactt ttttaagaaa aaagccggga aaattcccag ctttgctatt atattgatcc
     3241 cagcaggatt cgaacctgcg accgttcgct tagaaggcga atgctctatc cagctgagct
     3301 atgagaccta atacaattat tctaccaaaa attcaattaa aagtcaattt tctatttatg
     3361 gtaggggaat ccctgctgaa tcgtaaaagc gcgatagatt tgttcaacaa gaactagtct
     3421 cattaactga tggggtaagg ttaggcgacc aaaactgaca gaaagattgg ctctattttt
     3481 tacagatgat gacaatccta aacttccccc aataataaaa gtaagagtag aaaatccttt
     3541 tatagaagtt tcttctaact gcttactaaa ttcttctgag aagaaagttt tcccttcaat
     3601 ggctaacaca ataacgaaat cacggtcagc aatttttgat aaaattctct gaccttctat
     3661 ttctaaaatc ttttgatttt ctgattcact ggccttatct ggtgtttttt catctgataa
     3721 ctcaatcatt tcaaacttag caaatctaga aattcgtttt gaatactctg cgataccatc
     3781 ttttaaatac ttttctttca gtttcccaac tgttacaact ttaattttca tgactctatt
     3841 ctaacatatt ctctattttt tcacatctta ttcacaaaat aaaaaataga tttcaattaa
     3901 gaaaatcaca gtttcaaaag agttatccac agtttgtgta aaacttttgt gtttaagtta
     3961 taattaagct agtcagttta tactttcagt aattcaaaca tatggaggca aatatgaaac
     4021 atctaaaaac attttacaaa aaatggtttc aattattagt cgttatcgtc attagctttt
     4081 ttagtggagc cttgggtagt ttttcaataa ctcaactaac tcaaaaaagt agtgtaaaca
     4141 actctaacaa caatagtact attacacaaa ctgcctataa gaacgaaaat tcaacaacac
     4201 aggctgttaa caaagtaaaa gatgctgttg tttctgttat tacttattcg gcaaacagac
     4261 aaaatagcgt atttggcaat gatgatactg acacagattc tcagcgaatc tctagtgaag
     4321 gatctggagt tatttataaa aagaatgata aagaagctta catcgtcacc aacaatcacg
     4381 ttataaatgg cgccagcaaa gtagatatta gattgtcaga tgggactaaa gtacctggag
     4441 aaattgtcgg agctgacact ttctctgata ttgctgtcgt caaaatctct tcagaaaaag
     4501 tgacaacagt agctgagttt ggtgattcta gtaagttaac tgtaggagaa actgctattg
     4561 ccatcggtag cccgttaggt tctgaatatg caaatactgt cactcaaggt atcgtatcca
     4621 gtctcaatag aaatgtatcc ttaagatcgg aagatggaca agctatttct acaaaagcca
     4681 tccaaactga tactgctatt aacccaggta actctggcgg cccactgatc aatattcaag
     4741 ggcaggttat cggaattacc tcaagtaaaa ttgctacaaa tggaggaaca tctgtagaag
     4801 gtcttggttt cgcaattcct gcaaatgatg ctatcaatat tattgaacag ttagaaaaaa
     4861 acggaaaagt gacgcgtcca gctttgggaa tccagatggt taatttatct aatgtgagta
     4921 caagcgacat cagaagactc aatattccaa gtaatgttac atctggtgta gttgttcgtt
     4981 cggtacaaag taatatgcct gccaatggtc accttgaaaa atacgatgta attacaaaag
     5041 tagatgacaa agagattgct tcatcaacag acttacaaag tgctctttac aaccattcta
     5101 tcggagacac cattaagata acctactatc gtaacgggaa agaagaaact acctctatca
     5161 aacttaacaa gagttcaggt gatttagaat cttgattgac atctatgtaa agaaagcttt
     5221 acataagaga aaagatgtgt tagtgtagaa tcatggaaaa atttgaaatg atttctatca
     5281 cagatataca aaaaaatccc tatcaacccc gaaaagaatt tgatagagaa aaactagatg
     5341 aactagcaca gtctatcaaa gaaaatgggg tcattcaacc gattattgtt cgtcaatctc
     5401 ctgttattgg ttatgaaatc cttgcaggag agagacgcta tcgggcttca cttttagctg
     5461 gtctacggtc tatcccagct gttgttaaac agatttcaga ccaagagatg atggtccagt
     5521 ccattattga aaatttacag agagaaaatt taaacccaat agaagaagca ctcgcctatg
     5581 aatctctcgt agagaaagga ttcacccatg ctgaaattgc agataagatg ggcaagtctc
     5641 gtccatatat cagcaactcc attcgtttac tttccttgcc agaacagatt ctttcagaag
     5701 tagaaaatgg caaactatca caagcccatg cgcgttccct agttgggtta aataaggaac
     5761 aacaagacta tttctttcaa cggattatag aagaagatat ttctgtaagg aaattagaag
     5821 ctcttctgac agagaaaaaa caaaagaaac agcaaaaaac taatcatttc atacaaaatg
     5881 aagaaaaaca gttaagaaaa ctactcggat tagatgtaga aattaaacta tctaaaaaag
     5941 acagtggaaa aatcattatt tctttttcaa atcaagaaga atatagtaga attatcaaca
     6001 gcctgaaata aggctgttct tttatttttt tatctcacaa ggttatccac tatgtttttc
     6061 gataaaaagc ttaataaatc aataatttct tcttttatcc ccaacctgtg gataaagttt
     6121 ggtaacattg tggattattt ttcacagctt gtggaaaatt cttgctatct atggtaaaat
     6181 atctctagta ttaaactttt aaatagtaaa ggaggagaaa ggattgaaag aaaaacaatt
     6241 ttggaatcgt atattagaat ttgcacaaga aagactgact cgatccatgt atgatttcta
     6301 tgctattcaa gctgaactca tcaaggtaga ggaaaatgtt gccactatat ttctacctcg
     6361 ctctgaaatg gaaatggtct gggaaaaaca actaaaagat attattgtag tagctggttt
     6421 tgaaatttat gacgctgaaa taactcccca ctatattttc accaaacctc aagatacgac
     6481 tagctcacaa gttgaagaag ctacaaattt aactctttat aactatagtc caaagttagt
     6541 atctattcct tattcagata cgggattaaa agaaaagtat acctttgata actttattca
     6601 aggggatgga aatgtttggg ctgtatcagc cgctttagct gtctctgaag atttggctct
     6661 gacctataac cctcttttta tctatggagg accaggcctt ggtaagactc acttattaaa
     6721 cgctattgga aatgaaattc taaaaaatat tcctaatgcg cgtgttaaat atatccctgc
     6781 cgaaagcttt attaatgact ttcttgatca cctaagactt ggggaaatgg aaaagtttaa
     6841 aaagacctat cgtagtcttg atcttttgtt aatcgatgat attcagtcac tcagcggaaa
     6901 aaaagttgca actcaggaag aatttttcaa tacctttaac gcccttcatg acaagcaaaa
     6961 acagattgtc ctaacgagtg atcgtagtcc aaaacatcta gaagggctcg aggagaggct
     7021 tgtcacgcgt tttagttggg gattgacaca aactatcaca ccccctgact ttgaaacacg
     7081 tattgccatt ttacaaagta aaacggaaca tttaggctac aatttccaaa gtgatactct
     7141 agaataccta gctgggcaat ttgattcaaa tgttcgagat cttgagggag ccatcaacga
     7201 catcacttta attgccagag taaaaaaaat caaggatatc actattgata ttgctgcaga
     7261 agccattaga gcccgcaaac aagatgttag ccaaatgctc gtcatcccaa ttgataaaat
     7321 ccaaactgaa gttggtaact tttatggtgt tagtatcaaa gaaatgaagg gaagtagacg
     7381 ccttcaaaat attgttttgg cccgtcaagt agccatgtat ttatctagag aactaacaga
     7441 taatagtctt ccaaaaattg ggaaggaatt tgggggaaaa gatcatacca cagtcattca
     7501 tgcccatgcc aaaataaaat ctttgattga tcaagacgat aatttacgtt tagaaattga
     7561 atcaatcaaa aagaaaatca aataatttgt ggataacttt tagtttttta tcttttttat
     7621 ccacattttt taaacaagct aaaaaacttg atatgacttg tttaaaggct gttttccaca
     7681 gat
//
LOCUS       DBERCA010000069         7567 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.069, whole genome shotgun sequence.
ACCESSION   DBERCA010000069 DBERCA010000000
VERSION     DBERCA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.069"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            561..1013
                     /locus_tag="ACL719_001789"
     CDS             561..1013
                     /locus_tag="ACL719_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS0699709.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1032..2369
                     /locus_tag="ACL719_001790"
     CDS             1032..2369
                     /locus_tag="ACL719_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0699710.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2448..3332
                     /locus_tag="ACL719_001791"
     CDS             2448..3332
                     /locus_tag="ACL719_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0699711.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3332..4165
                     /locus_tag="ACL719_001792"
     CDS             3332..4165
                     /locus_tag="ACL719_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0699712.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4183..6276
                     /gene="nanB"
                     /locus_tag="ACL719_001793"
     CDS             4183..6276
                     /gene="nanB"
                     /locus_tag="ACL719_001793"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HGS0699713.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSSSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6288..7391
                     /locus_tag="ACL719_001794"
     CDS             6288..7391
                     /locus_tag="ACL719_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS0699714.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
BASE COUNT         2528 a         1148 c         1539 g         2352 t
ORIGIN      
        1 taacaaaact aatttagaga atgaaatata gagtatttct ctcttaaaag tttttggtga
       61 aacgagatgt agaaaggaga tttagccaaa gagtctatta gtgctagaat aatagattag
      121 aattatttta gaaaaacgaa gtgagcagct tataaattca agtccccaaa tagattcata
      181 ctagtatctt ttgcaaaaaa taaagggcga cttccttcat gaatatcaat ttcatctata
      241 aggaaggtag ctaattgaac taacttattt attctgtttg tcgctagaaa aatcagacct
      301 ccttgtgaag attgaggaga tacttaatga aaatcaaaga agaaactagc aagctagtag
      361 cagattgccc aaaacaccgc tttgaggttg tagataagac tgacctatat aatccaaggt
      421 gaagcgactg tggtttgaag agattttcaa agagtatagg ctagagagta gtgtttttat
      481 gtccttctag tagaaaatgc tagacagaag aatggggaac ttggatagga aaaatagatt
      541 gagaaaggag gttagaagag atgattatta caaaaattag ccgtttagga acttatgtgg
      601 gagtaaatcc acattttgca acattaatag attttctaga aaaaacagga ctagaaaatt
      661 taacagaagg ttcgattgct atcgatggta atcgattgtt tgggaattgc tttacttatc
      721 tagcagatgg tcaagcaggg gctttctttg aaacccacca aaaatatttg gatattcatt
      781 tagttttgga aaacgaagaa gccatggctg ttacatcgcc ggaaaatgta agcgttaccc
      841 aagaatatga tgaagagaaa gatattgaat tatacacagg gaaagtggaa cagttggttc
      901 atttgagagc tggcgaatgc ctcatcactt ttccagaaga tttacatcaa cccaaggttc
      961 gtataaatga tgaacctgtg aaaaaagttg tctttaaagt tgcgatttct taatgtagaa
     1021 agagaagaac gatgaaaaaa atgagaaagt ttttatgtct agctggaatt gcgctagcgg
     1081 ctgttgcctt ggtagcttgt tcaggaaaaa aagaagctac aactagtact gaaccaccaa
     1141 cagaattatc tggtgagatt acaatgtggc actcctttac tcaaggaccc cgtttagaaa
     1201 gtattcaaaa atcagcagat gctttcatgc aaaagcatcc aaaaacgaaa atcaagattg
     1261 aaacattttc ttggaatgac ttctatacta aatggactac aggtttagca aatggaaatg
     1321 tgccagatat cagtacagct cttcctaacc aagtaatgga aatggtcaac tcagatgctt
     1381 tggttccgct aaatgattct atcaagcgta ttggacaaga taaatttaac gaaactgcct
     1441 taaatgaagc aaaaatcgga gatgattact actctgttcc tctttattca catgcacaag
     1501 tcatgtgggt tagaacagat ttgttaaaag aacataatat tgaggttcct aaaacttggg
     1561 atcaactcta tgaagcttct aaaaaattga aagaagctgg agtttatggc ttgtctgttc
     1621 cgtttggaac aaatgactta atggcaacac gtttcttgaa cttctacgta cgtagtggtg
     1681 gaggaagcct cttaacaaaa gatcttaaag cagacttgac aagccaactt gctcaagatg
     1741 gtattaaata ctgggttaaa ttgtataaag aaatctcacc tcaagattct ttgaacttta
     1801 atgtccttca acaagctacc ttgttctatc aaggaaaaac agcatttgac tttaactctg
     1861 gcttccatat cggaggaatt aatgccaaca gtcctcaatt gattgattcg attgatgctt
     1921 atcctattcc aaaaatcaaa gagtctgata aagaccaagg aattgaaacc tcaaacattc
     1981 caatggttgt ttggaaaaat tcaaaacatc cagaagttgc taaagcattc ttagaagcac
     2041 tttataatga agaagactac gttaaattcc ttgattcaac tccagtaggt atgttgccaa
     2101 ctattaaggg gattagcgat tctgcagcct ataaagaaaa tgaaactcgt aagaaattta
     2161 aacatgctga agaagtaatt actgaagctg ttaaaaaagg tactgctatt ggttatgaaa
     2221 atgggccaag tgtacaagct ggtatgttga ctaaccaaca cattattgaa caaatgttcc
     2281 aagatatcat tacaaatgga acagatccta tgaaagcagc aaaagaagca gaaaaacaat
     2341 taaatgattt atttgaggct gttcagtaga tgtaaaagac tagaaaatag gtgggatagt
     2401 gagctgaaaa gctctagccc aatcttgtaa aagaagggag aaggagaatg gttaaagaac
     2461 gtaatttaac tcgctggata tttgttttgc cagctatgat tatcgtagga ttactctttg
     2521 tttatccgtt tttctcgagt attttttata gctttaccaa taagcatttg attatgccta
     2581 attataaatt tgttggtttg gctaactata aagctgtgct atcagatccc aacttcttta
     2641 atgcgttctt taattcaatt aagtggaccg ttttctcatt agttggtcaa gttttagtag
     2701 ggtttgtatt ggctttagct cttcacagag tacgccactt caagaaatta tataggacat
     2761 tattgattgt tccttgggca tttcctacca tcgttattgc cttctcttgg cagtggattc
     2821 taaacggggt ttatggctac ttacctaatc taatcgtaaa attaggttta atggaacata
     2881 cacctgcatt tttgacagat agtacatggg cattcctatg tttggtgttt atcaacattt
     2941 ggtttggagc accaatgatt atggttaatg tgctttcagc tttgcaaaca gtaccagaag
     3001 aacaatttga ggctgctaag atagatggtg cttcaagttg gcaggtgttc aagtttatcg
     3061 tctttccaca tattaaagtg gttgtaggac ttctagttgt tttgagaact gtatggatct
     3121 ttaataactt tgacattatc tacctcatta ctggtggtgg accagccaat gctacaacga
     3181 cgcttccaat ttttgcttac aacctgggct ggggaactaa attgttgggt cgtgcttcag
     3241 cagttacagt attgctcttt atcttcttgg tggcgatttg ctttatctac tttgctatca
     3301 tcagtaagtg ggaaaaggag ggtagaaaat aatgaagaag aaatccagta tttatttaga
     3361 tattctctca catgtacttt tagttggtgc gaccatcgtt gcaattttcc cattggtatg
     3421 gattatcata tcttctgtca aagggaaagg ggaattaact cagtatccaa cacgattttg
     3481 gcctgaacag tttacattag attatttcac tcatgttatc aacgatttgc acttcattga
     3541 taacattcga aacagtttaa tcattgcctt ggctacaacc cttattgcga ttattatttc
     3601 tgctatggca gcctatggta ttgttcgatt ctttcctaaa ttgggagcaa tcatgtcgag
     3661 actactcgtc attacctaca ttttcccacc aattttgtta gcaattccct attcaattgc
     3721 cattgctaaa gttgggttaa caaatagttt atttggcttg atgatggttt atctatcttt
     3781 tagtgttcca tatgcagttt ggctcttagt tggatttttc caaacagttc caattggaat
     3841 tgaagaagcg gctagaattg atggtgcaaa taaatttgtt acgttttata aagttgtgct
     3901 accgattgta gcaccaggta ttgtagcaac agctatttat acatttatca atgcttggaa
     3961 tgaattcctg tatgccttga ttttgattaa caatacagga aagatgacag tagcagtagc
     4021 ccttcgttca cttaatggtt cagaaatact agactgggga gatatgatgg cagcgtctgt
     4081 tattgtagtt cttccatcaa ttattttctt ctctatcatc caaaataaga ttgcaagtgg
     4141 attatcagaa ggatctgtga agtagacgaa agaaggaaaa aaatgaataa aagaggtctt
     4201 tattcaaaac taggaatttc tgttgtaggc attagtcttt taatgggagt ccccgctttg
     4261 attcatgcga atgaattaaa ctatggtcaa ctgtccatat ctcctatttt tcaaggaggt
     4321 tcatatcaac tgaacaataa gagtatagat atcagccctt tgttattaga taaattgtct
     4381 ggagatagtc agacagtagt aatgaaattt aaagcagata aaccaaactc tcttcaagct
     4441 ttgtttggcc tatctaatag taaagcaggc tttaaaaata attacttttc aattttcatg
     4501 agagattctg gtgagatagg tgtagaaata agagacgccc aaaagggaat aaattattta
     4561 ttttctagac cagcttcatt atggggaaag cataaaggac aggcagttga aaatacacta
     4621 gtatttgtat ctgattctaa agataaaaca tacacaatgt atgttaatgg aatagaagtg
     4681 ttctctgaaa cagttgatac atttttgcca atttcaaata taaatggtat agataaggca
     4741 acactaggag ctgttaatcg tgaaggtaag gaacattacc tcgcaaaagg aagtattgat
     4801 gaaatcagtc tatttaacaa agcaattagt gatcaggaag tttcaaatat tcccttgtca
     4861 aatccatttc agttaatttt ccaatcagga gattctactc aagctaacta ttttagaata
     4921 ccgacactat atacattaag tagtggaaga gttctatcaa gtattgatgc acgttatggt
     4981 gggactcatg attctaaaag taagattaat attgccactt cttatagtga tgataatggg
     5041 aaaacgtgga gtgagccaat ttttgctatg aagtttaatg actatgagga gcagttagtt
     5101 tactggccac gagataataa attaaagaat agtcaaatta gtggaagtgc ttcattcata
     5161 gattcatcca ttgttgaaga taaaaaatct gggaaaacga tattactagc tgatgttatg
     5221 cctgcgggta ttggaaataa taatgcaaat aaagccgact caggttttaa agaaataaat
     5281 ggtcattatt atttaaaact aaagaagaat ggagataacg atttccgtta tacagttaga
     5341 gaaaatggtg tcgtttatga tgaaacaact aataaaccta caaattatac tataaatgat
     5401 aagtatgaag ttttggaggg aggaaagtct ttaacagtcg aacaatattc ggttgatttt
     5461 gatagtagct ctttaagaga aaggcataat ggaaaacagg ttcctatgaa tgttttctac
     5521 aaagattcgt tatttaaagt gactcctact aattatatag caatgacaac tagtcagaat
     5581 agaggagaga gttgggaaca atttaagttg ttgcctccgt tcttaggaga aaaacataat
     5641 ggaacttact tgtgtcctgg acaaggttta gcattaaaat caagtaacag attgattttt
     5701 gcaacatata ctagtggaga actaacctat ctcatttctg atgatagtgg tcaaacatgg
     5761 aagaaatcct cagcttcaat tccgtttaaa aatgcaacag cagaagcaca aatggttgaa
     5821 ctgagagatg gtgtgattag aacattcttt agaaccacta caggtaagat agcttatatg
     5881 actagtagag attctggaga aacatggtcg gaagtttcgt atattgatgg aattcaacaa
     5941 acttcatatg gcacacaagt atctgcaatt aaatactctc aattaattga tggaaaagaa
     6001 gcagtcattt tgagtacacc aaattctaga agtggccgta agggaggtca attagttgtc
     6061 ggtttagtca ataaagaaga tgatagtatt gattggaaat accactatga tattgatttg
     6121 ccttcgtatg gttatgccta ttctgcgatt acagaattgc caaatcatca cataggtgta
     6181 ctgtttgaaa aatatgattc gtggtcgaga aatgaattgc atttaagcaa tgtagttcag
     6241 tatatagatt tggaaattaa tgatttaaca aaataaagga gaaaaacatg gttaaatacg
     6301 gtgttgttgg agcagggtat tttggagctg aattggctcg ctatatgcaa aagaatgatg
     6361 gagcagagat tactcttctc tatgatccag ataatgcaga ggcgattgca gaagaattgg
     6421 gagcaaaagt agcaagttcc ttagatgagt tggtttctag cgatgaagta gattgtgtta
     6481 tcgtcgcaac tccaaataat cttcataagg aaccggttat taaggctgca cagcatggta
     6541 aaaatgtttt ctgtgaaaaa ccaattgcgc tttcttatca agattgtcgc gagatggtag
     6601 atgcgtgtaa agaaaacaat gtaaccttta tggcaggaca tattatgaat ttctttaatg
     6661 gtgttcatca tgcaaaagaa ctcattaatc aaggagttat cggagacgtt ctatattgtc
     6721 atacagctcg taatggttgg gaagaacaac aaccgtcagt atcatggaaa aaaattcgtg
     6781 aaaaatcagg tggtcacttg tatcaccaca tccatgaatt ggattgcgtt caattcctta
     6841 tggggggcat gcctgaaact gtaaccatga caggtggaaa tgtggcccat gaaggtgaac
     6901 atttcggtga tgaagatgat atgatttttg tcaatatgga attttctaat aagcgttttg
     6961 ccttgttaga atggggttca gcttatcgtt ggggtgaaca ttatgtctta atccaaggaa
     7021 gcaaaggtgc catccgctta gacttattca actgtaaagg aactcttaag ctagatgggc
     7081 aagaaagcta tttcttgatt cacgaatcgc aagaagaaga tgatgatcgg actcgtatct
     7141 atcatagtac agagatggat ggagcaattg cttatggtaa accaggtaaa cgtactccat
     7201 tatggctatc atctgtcatt gataaagaaa tgcgctatct gcatgagatt atggaaggag
     7261 ctccagtatc agaagaattt gcaaaacttt tgacaggtga agctgcccta gaagcaattg
     7321 ctactgcaga tgcttgtacc cagtctatgt ttgaagatcg caaagtaaaa ttgtcagaaa
     7381 ttgtaaaata aattttggta ttctcctatt tataggtcga cttgctcctc tgaaagtact
     7441 tttagaggag ctgtttgact ttgctagttt ttgaaactga aatctattat actacaaact
     7501 attgaaagcg ttttaatttt aaggtataat aatctcatag aaataaagaa aaggaggaaa
     7561 gaggatg
//
LOCUS       DBERCA010000070         7466 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.070, whole genome shotgun sequence.
ACCESSION   DBERCA010000070 DBERCA010000000
VERSION     DBERCA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.070"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            129..284
                     /locus_tag="ACL719_001795"
                     /pseudo
     CDS             129..284
                     /locus_tag="ACL719_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            484..3759
                     /gene="rexB"
                     /locus_tag="ACL719_001796"
     CDS             484..3759
                     /gene="rexB"
                     /locus_tag="ACL719_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGS0699715.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3756..7406
                     /gene="addA"
                     /locus_tag="ACL719_001797"
     CDS             3756..7406
                     /gene="addA"
                     /locus_tag="ACL719_001797"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGS0699716.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2277 a         1569 c         1585 g         2035 t
ORIGIN      
        1 actatattag attgaaataa gatacgaaca gcttgattag ggaaatcaaa ttaatttcta
       61 gaaatatttt agcatccacg atgtactatt atagtttcaa tttactatag ttcaatcatt
      121 ctatcaagaa tattgaatat ataaaggcac gtaatattaa aacttcttct gatatcgaat
      181 tagagtccga tgttgatggc gataaatcag acaatctacc cgtcgacatt aagatactgg
      241 gacaacacat tgaagtgtat tccgtaccta agaaacaatc ataaaactca aagtcttgtt
      301 accgaggctt ttttattatg tgattttcag tagccagact tattctatct aatacaagta
      361 aagctaagta tttaagattt agcagatgat gaagaaaaga aaggatggcc taaatatttt
      421 aacagattta cgaggctaat catggtataa tatagggtga tgaaaaattc caacgaggct
      481 gagatgaaat tactttatac tgatattcga acttctttga cagaaattct aacaagagag
      541 gcagaagagc tagttgcagc tggcaagcgg gtcttctaca ttgcccccaa ctctctttct
      601 tttgaaaagg aacgcgccgt gctggaatgc ttgtcccagc aggcttcttt ttctattacc
      661 gtcacgcgct ttgctcaaat ggctcgttat ctggtcctga atgatttacc agttaaaact
      721 actcttgatg atatcggtct tgggttggcc ttttacaaat gccttgccga actcgatccc
      781 aaggacttgc gtgtttatgg cgctattaag caggatcctc aattgatcca gcagttaatt
      841 gagctttacc atgagatgac caaatctcag atgagttttt tggacttgga gaatttaaca
      901 gatgaggata agaggacgga tttactcttg atttttgaga aagtaacagc ctatcttaat
      961 caaggtcagt tagcccaggg aagtcagttg tcccatttga ttgaggctat tgagaatgac
     1021 aaggtaagta gtgattttaa tcaaatcacc ttggtcattg acggctttac tcgtttttct
     1081 gctgaggaag agcgggttgt ggacttactt cacggcaaag gtgttgagat tgttatcggg
     1141 gcttatgcta gtaagaaagc ctataccagt ccttttagcg agggcaatct ctaccaagcc
     1201 agcgtaaaat ttctccatca tctggcttct aaataccaaa cgcctgctca ggactgttct
     1261 caaactcatg agaagatgga tagttttgac aaggcctctc gtttgttgga gtcttcttat
     1321 gacttttcag aactcgcttt ggatgtcgat gagaaagacc gtgaaaattt acaaatctgg
     1381 tcttgtttga cgcaaaagga ggagttggag ctagtagccc gtagcattcg tcagaaatta
     1441 catgagaact cagacctgag ctacaagcat tttcgtattc tcttggggga tgtagcttct
     1501 taccagttat ctctcaaaac catttttgac cagtatcaga ttccttttta tcttggtaga
     1561 agcgaagcca tggctcatca tcccttgact cagtttgtcg agtctatttt agctttaaaa
     1621 cgttaccgtt ttcgtcagga ggatttgatt aatcttctta gaactgattt gtatactgac
     1681 ctcagtcagt ctgatattga tgcttttgag caatatatcc gctatcttgg tatcaatggc
     1741 ttgccagcct ttcagcaaat cttcaccaaa tcccaccatg gaaaatttaa tcttgagcgt
     1801 ttgaatgtcc tccgcctgag aattttagca cctcttgaaa ccctctttgc cagccgaaaa
     1861 caaaaggctg aaaacctcct acaaaaatgg agtgtctttc taaaagaagg agctgtgacc
     1921 aagcagttac aagatttgac aaccactttg gaagctgtag aacaggaaag acaagccgaa
     1981 gtttggaagg ctttctgcca tgttttagaa caatttgcga ctgtttttgc tggttcacag
     2041 gttagtctgg aagacttcct agccttgctc cattctggaa tgagtttgtc ccaataccgt
     2101 accattccag caacagtgga cactgttctg gtgcagagtt acgatttgat tgcaccattg
     2161 actgctgact ttgtctatgc tattggacta actcaggaca atttaccaaa aatttctcaa
     2221 aacaccagtc ttctgacaga tgaagaaagg caaaatctaa accaagcgac cgaagaaggc
     2281 gttcaattac tgattgccag cagtgaaaat ctcaagaaaa atcgctacac tatgctttcc
     2341 ttggtcaatt ctgctcgtaa gcagttgttc ttgtcggctc caagcctttt taacgaaagt
     2401 gaaagtaagg aatctgccta tcttcaagag ttgatccatt ttggatttag gcggagagag
     2461 aagaggatga atcacaaagg actgtctaag gaggatatgg ggtcctatca cagtcttttg
     2521 tctagtctgg ttgcctatca ccagcagggt gagatgagcg atactgagca agatttgact
     2581 tttgtcaagg ttctgtcgcg tgtcataggt aaaaaactag atctgcaagg tctggaaaat
     2641 ccagctatcc caaccagtcc aagcagcaag accttagcca aggacacctt gcaagctctc
     2701 tatcctgcca aacaggagtt ttacctgtct acgtcgggtt tgacagagtt ttatcgcaat
     2761 gaatacagtt atttcctacg ctacgtttta ggcttgcagg aggaattacg tttgcgtcct
     2821 gatgcccgta gtcacgggaa tttcttgcat cgtatctttg aacgcgcctt acagttgcct
     2881 aatgaagatt cctttgacca acgtctagaa caagctattc aagaaaccag tcaagaacgc
     2941 gaatttgaag ctatttatca agaaagtttg gaagcccagt ttaccaagga agttttgctt
     3001 gatgttgcac ggacgactgg acatattctc cgacacaatc cagccatcga aaccatcaaa
     3061 gaagaagcaa attttggtgg aaaagaccaa gcctttattc aattagacaa tggacgcagt
     3121 gtctttgtac gaggcaaggt ggaccggatt gaccgtttga aagctaatgg agcgatagga
     3181 gtagtagact acaaatccag tctgactcag ttccagtttc ctcatttctt taatgggctc
     3241 aattctcagt taccaaccta tcttgctgcc ctaaaaagag aaggggagca gaactttttc
     3301 ggcgccatgt acttggaaat ggctgaacct gtccaatctc tgatggcggt aaaaagtctg
     3361 gcaggagcag tggtagaagc cagcaaatct atgaaatacc aagggctctt cttggaaaaa
     3421 gaaagcagtt atttaggcga attttataac aaaaacaagg ctaatcaact gacagatgag
     3481 gaatttcagc tcctactgga ctacaatgcc tatctttaca agaaagctgc tgagaagatt
     3541 ttagcaggcc ggttcgccat caatccttat actgaaaatg gcagaagcat tgccccatac
     3601 gtccagcaac atcaggctat tacaggcttt gaagccaatt accatctggg ccaagcccgt
     3661 ttcctagaaa agttggacct agctgatggc aagcgtctgg tcggagaaaa actcaagcaa
     3721 gcttggtttg aaaaaataag agaggagtta aatcgatgaa gcttattccc tttttaagtg
     3781 aggaggagat tcaaaaactg caagaagcag aagcaaattc gagcaaggaa cagaagaaaa
     3841 ctgccgaaca aatcgaagct atctacactt ctggtcagaa tatcctggtc tcagcatcgg
     3901 ctggttctgg aaagaccttt gtcatggtag agcgcattct ggaccaattg gcgcgtggtg
     3961 tcgaaatttc tcaactcttt atctcaacct ttaccgtcaa ggctgcaact gaacttaaag
     4021 aacgtttaga gaaaaaaatc agcaagaaaa tccaagaaac agatgatgtc gatctcaaac
     4081 aacacttggg tcgccagttg gcagacctac ccaacgctgc catcggaacc atggactctt
     4141 tcacacaaaa attccttggc aaacatggtt atctgcttga tattgcacct aatttccgta
     4201 ttttacaaaa ccaaagcgag caacttcttc tcgaaaacga agtctttcat gaggtctttg
     4261 aagcgcatta ccaaggtaaa cagaaagaga gctttagtca tttgctgaaa aactttgctg
     4321 ggcgtggcaa ggacgaacgg ggtctgcggc agcaagtcta taaaatctat gacttcctcc
     4381 aatccaccag taatcctcaa aagtggctga gtgaatcttt cctcaaaggc tttgagaaag
     4441 ctgattttac cagtgaaaaa gaaaaactga ctgagcaaat caaacaagcc ctctgggatt
     4501 tggaaagctt tttccgttac catctggata acgatgccaa ggagtttgca aaggctgcct
     4561 atttagaaaa tgttcagtta attctggatg aaattggctc cctaaatcag gagttcgata
     4621 gtcaggctta tcaggcagta cttgcgcgtg ttgtcgccat ctctaaggag aaaaacggtc
     4681 gagctctgac taatgccagc cgtaaggctg atttgaagcc cctggctgat gcctacaacg
     4741 aagagagaaa gacccagttt actaaactag gacaattatc agaccagata gcgattctcg
     4801 actatcaaga acgttatcat ggagacactt ggaaactagc taaaaccttc caatctttca
     4861 tgagcgattt tgtagaggct tatcgtcaga gaaaacgaca ggaaaatgcc tttgaattcg
     4921 ctgatatcag ccattacacc attgagattt tagaaaattt cccacaagtt cgtgagtctt
     4981 atcaggagcg cttccatgaa gtcatggtcg atgagtatca ggatatcaac catattcaag
     5041 aacggatgct ggaattgttg tctaatggcc acaatcgctt tatggtggga gatatcaagc
     5101 aatccatcta tcgtttccgt caggcagacc cgcagatttt caatgagaaa ttccaacgct
     5161 atgcgcaaaa tcctcaagaa ggcaagctca ttctcctcaa ggaaaatttc cgtagtagtt
     5221 cagaagtgct gtcagcaacc aatgatgtct ttgaacgtct catggaccaa gaggtcggcg
     5281 aaatcaacta tgataacatg caccagcttg tttttgccaa taccaaactg actcccaatc
     5341 cagacaacaa ggccgaattt ctcctctacg acaaggacga tacaggtgag gaagaagaga
     5401 gtcaaacaga aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag atccttaaac
     5461 ttcatcagga aaaaggtgtt gcctttaagg aaattgccct tctgacctct agtcgtaatc
     5521 gtaatgacca aattctactt gccctgtctg agtacgggat tcctgtcaaa actgacggag
     5581 agcaaaacaa ttatctccaa tccctagaag tgcaagtcat gctagacacc cttcgtgtca
     5641 ttcacaatcc cctgcaagac tacgccttgg ttgcccttat gaagtctcca atgtttggtt
     5701 ttgatgagga tgagctagca cgtttgtccc ttcagaaagc agaggataaa gcccacgaaa
     5761 atctctatga gaaactagtc aatgcacaaa aaaaggcaag tagtcaaaaa ggcttgattc
     5821 acacagctct agctgaaaaa ctaaagcaat tcatggatat cctagcttct tggcgcttgt
     5881 atgccaaacc ccactctctc tatgacttga tttggaagat ttacaacgac cgtttttatt
     5941 atgactatgt tggggctttg ccgaatggtc ctgctaggca ggccaatctc tatgccctag
     6001 cactgcgtgc tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt tttattcgta
     6061 tgattgacca agtcttagaa gcccagcacg atttggcaag cgtggccgtc gcaccgccaa
     6121 aagatgcagt agagctcatg accatccaca agagtaaagg gctggagttt ccttacgtct
     6181 ttatcctcaa tatggatcaa gatttcaaca aacaagactc tatgtcagaa gtcattctca
     6241 gtcgtcagaa tggtcttggt gtcaaatata ttgccaagat ggagacaggg gcagtagaag
     6301 accactatcc taaaaccatc aaactctcca ttcctagtct gacctatagg cagaacgaag
     6361 aggaattaca gctagcaagc tattctgagc agatgcgttt gctgtatgtt gctatgacgc
     6421 gggctgagaa aaagctctat cttgtcggca agggttctcg tgaaaagctg gaatccaagg
     6481 aatacccagc agccaaaaat gggaaactaa atagcaatac tagactgcaa gcacggaatt
     6541 tccaagattg gctttgggct atcagtaaag tgtttactaa ggacaagctc aactttagtt
     6601 atcgttttat tggcgaagat cagttgacca gagaagctat cggagagttg gaaaccaaga
     6661 gtcctctcca agatagctcc caagcagaca atcgtcagtc agataccatc aaagaagctc
     6721 tggaaatgct gaaggaggtg gaagtttata atactcttca ccgcgcagct attgaacttc
     6781 ctagtgttca aaccccaagt caaatcaaga aattctacga accagttatg gatatggaag
     6841 gtgtcgagat tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc gatttgccag
     6901 atttttcaac caaagaaaag gtaactggag ctgagattgg tagtgctact cacgaactca
     6961 tgcagagaat tgacctcagc cagcaactaa cccttgctag cctaacagaa acactcaaac
     7021 aagttcaaac tagccaagct gtcagagaca agatcaatct tgataaaatt cttgctttct
     7081 ttgacacagc actcggtcag gaaatttttg ctaataccga ccatctctat cgcgagcaac
     7141 ctttctccat gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc cgtggtatcc
     7201 ttgatggcta tctgctttac gaaaacaaaa ttgttttgtt cgactacaag acagaccgct
     7261 atgatgaacc aagtcaactc gtagaccgct atcgtggtca gttagctcta tacgaagagg
     7321 ctttatcacg agcctattcg attgaaaata ttgaaaaata cttgatttta ctcggtaaag
     7381 acgaggttca agttgtaaaa gtataactta tactcttcga aaatctcttc aaaccacgtc
     7441 agcttcgcct tgctgtatat atggtt
//
LOCUS       DBERCA010000071         7399 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.071, whole genome shotgun sequence.
ACCESSION   DBERCA010000071 DBERCA010000000
VERSION     DBERCA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.071"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            226..6657
                     /locus_tag="ACL719_001798"
     CDS             226..6657
                     /locus_tag="ACL719_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS0699717.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPHEVVLADTSNSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDNNTSNEEARIKE
                     DSNQSQGDNAHSSANKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSSLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLTGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAY
                     EDESALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYINGKEYTSFDDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REEHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
     gene            7284..>7399
                     /locus_tag="ACL719_001799"
     CDS             7284..>7399
                     /locus_tag="ACL719_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0699718.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTP"
BASE COUNT         2916 a          994 c         1415 g         2074 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggagt ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctca tgaggtagtc
      301 ttagcagaca catctaactc tgaagatgct ttaagcatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa cataaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca attattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatctcaag gagataatgc acactcgtct gcaaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccagtcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcta tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaaggt
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttagggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctgata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttacggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacactgatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataacgaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgacaaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagacc taaagaaact cattaaaaag
     4441 aaatataagg aagaagacga ctttgtaact ggtggaaaaa gaacagtaga acttgattat
     4501 aaatatgacg ataaaggaaa tataactgcc tatgaagatg aaagtgctct agaatatgaa
     4561 actgaaaaat ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaataact ataaatttat agaaatcaaa gcgactaagt atgactcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa aattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga cctttcaaaa aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagtga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatataaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa tagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagagcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagat
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcaaggaa ttaagtagcg tttggttgct taattccttt ttatttggaa
     6961 ggaagttttt cgtaaccgcc caataacgaa gtctattgga cggttacgat atgatcactg
     7021 acttcgtcag tcttatctac aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc
     7081 ttagcttact ctttgatttt cattgagtat tagaacagaa aaaatgcttg gagtatttgt
     7141 ttgtgtgttt gtttttatat aacaaactat aaacaaaaca aaaatataaa aaaagagaca
     7201 aaaaagaaca gaaagtaatt gacaacggtt tcatatagtg ttatacttat ggcataaagt
     7261 taatggaaag aagggaaaac cttatggtat tagattattt ctttgacaaa aaccttgtgt
     7321 tttgcttaga agcggataat caagaacaac tttttgatca ggtagcaagc ttgttggaag
     7381 aacgagaaat tgtcactcc
//
LOCUS       DBERCA010000072         7054 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.072, whole genome shotgun sequence.
ACCESSION   DBERCA010000072 DBERCA010000000
VERSION     DBERCA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.072"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..774)
                     /locus_tag="ACL719_001800"
     CDS             complement(<1..774)
                     /locus_tag="ACL719_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS0699719.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYC"
     gene            934..2688
                     /locus_tag="ACL719_001801"
     CDS             934..2688
                     /locus_tag="ACL719_001801"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699720.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            2672..4393
                     /locus_tag="ACL719_001802"
     CDS             2672..4393
                     /locus_tag="ACL719_001802"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699721.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            4404..4988
                     /locus_tag="ACL719_001803"
     CDS             4404..4988
                     /locus_tag="ACL719_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS0699722.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4988..5683
                     /locus_tag="ACL719_001804"
     CDS             4988..5683
                     /locus_tag="ACL719_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0699723.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            5671..7035
                     /locus_tag="ACL719_001805"
     CDS             5671..7035
                     /locus_tag="ACL719_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699724.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKVEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         2540 a          936 c         1259 g         2319 t
ORIGIN      
        1 acaatatttt tccttaaata aattttttaa tttagtacca ctcattttag atattttttc
       61 aagcgtgcct tgatttacat tcgttgcaaa atgatcatct aagaatcttg ctacatcttc
      121 aagtgcttta tcatcatcaa tttcaatttt atattttttt ctatttaagt atgtgtcaat
      181 tactatactt atccattcat ttgcctttgc tttaaagaaa aaatcagcgg caggagcgtc
      241 catcttacaa tttaatattt ccattgccac tctttctaag gcctttgtaa gtattatttg
      301 attcggttga agcaaggttg aataaaaaga ttctggatta atgttaatag atgctaaatg
      361 tttttctatt agctcttttt taaaaccaat ggaaacagca agataacaac aattctcgtg
      421 taataaaaaa acaaaatcat cttttatatt atcaaaatca aaagtacata gagagtttgc
      481 ggtaatagtt tgatacggat taaacttttc tccgtttgca ctgacaatgt aacttgaata
      541 aattgaaaca tagtctgaca tactataagt gctattttga actacttcct ctttgatata
      601 aaaatcatgt atatcgataa tgaagctgcc tccttcataa aaccaatata gtccttctgc
      661 ataagttgaa ttctcttttt tccaacaaaa tgtatgacct gcacttgaat acttctcatt
      721 gtttttacat tcatctactt tcatataatc ttttacaaaa tcataatatg acataacaca
      781 ctatcccttt tagacaatat tccaattagc cttattaatt caaaactatt gtattagtaa
      841 ttataacaga tgtataatag aaaagcaatg atagatatta tcaattaagc gaatttatat
      901 ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa actatttgct
      961 tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc tatatctgct
     1021 ctacttacag tatatggata ttatttaatc tacaaatttc tagataagtt aataattaat
     1081 tcaaacttat ccggtgcaga gagtatagca ttaaaatctg ttattacact aacaagtgga
     1141 gcgatatttt attttgtctc aggaatgttt tcacatatct tgggatttag gcttgaaaca
     1201 aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt ctttgactta
     1261 aatccatctg gtcaaataag aaagatcata gatgataatg cagcacaaac tcatcaggtg
     1321 gtagcacaca tgattcccga tagttctcag gcaataatca cacccgtact tgtacttgca
     1381 cttggcttta tagtaagtat aagggttggc ataattttgc ttgctcttac tataattggt
     1441 ggcttaattt taggggcaat gatgggcgag caagaattta tgaagatata ccaagaatcc
     1501 ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt tgtaaaaata
     1561 tttaaagcaa atgtagagtc ttttaaaagc ttttataagg cgataaaaga ttactcaaag
     1621 tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca atggttattt
     1681 tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag cttagctagc
     1741 gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt tctctttgtt
     1801 tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta tgcagtagat
     1861 actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg taatgtcaat
     1921 aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa tgataaagct
     1981 gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact tgtcggttca
     2041 tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa tgttaataaa
     2101 ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc cttaattaaa
     2161 gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta tgataatgta
     2221 gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa attagcagga
     2281 tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg ctcaaaaggt
     2341 gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat tttaaaggat
     2401 tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa cgagtttgaa
     2461 ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat tgcacacagg
     2521 ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa aattatagaa
     2581 agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct gcaagagatg
     2641 tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa aaagatttgc
     2701 tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat ttttcgttta
     2761 ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag ttttggaaaa
     2821 tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga tagcaatgta
     2881 tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag aaagtgcaga
     2941 tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact tttctaaaca
     3001 tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag agcatgcaat
     3061 gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa tatctgtaat
     3121 gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta ttttaagctt
     3181 tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat attatgatgt
     3241 cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg agattaaagc
     3301 atataattta tcgaaggata ttaaagatga cttatataaa aaaatggaag atagtgagag
     3361 agtacactta aaggcggaag taactacaat tttaactttg tctatatctt caatatttag
     3421 ctttatatct cttgctgttg tgatatttgt cggcgtaaat ctaattatta ataaagagat
     3481 aaattctctc taccttatag gatatttact agctgctatg aagataaaag actctttaga
     3541 tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag aaagattaaa
     3601 agaaattcaa aatcaagatc tacaagaagg cgatgactat agcttaaaaa aatttgatat
     3661 tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa atggtgtaag
     3721 ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct gcggtaaaac
     3781 aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa tcttaatcga
     3841 tggcaaagat ataaaagaaa tatcaacaga atctcttttt gataaggtct ctatagtttt
     3901 ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg gaaagcaaga
     3961 tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag attttataga
     4021 aaaaatggat aaaggtttcg atacagttat tggtgagaac ggagctgagc tatcaggagg
     4081 agaaagacaa agattatcaa tagccagagc cttcttaaaa gatgcgccga tattgatctt
     4141 agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag agtctttaaa
     4201 taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat ccatagaaaa
     4261 tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta agcatgaaga
     4321 gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa tggcagaaga
     4381 atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa gatttagtaa
     4441 gcatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt atgatgggct
     4501 tgattccaat attgttctta atatacccga cagtattagc catagttgca ggaactgttg
     4561 ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt ggtatgatat
     4621 caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta tcacttatag
     4681 taatgataat agcagaatta attagaaaga ttggtaatta taattcattt aaatacaata
     4741 tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa atgcttttag
     4801 caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat gttgatgtat
     4861 tagaaaagtt aataacttat cctcacatgg ctttagtagc cttaggtgct ttcttaggag
     4921 gaattcttgg agcatatata ggcaaggctc tattgaaaaa acactttgaa aaagcaggca
     4981 ttgtataatg cctacttttt cttcaaatgc tccctttaat cccaatccga tctctaagat
     5041 attagtcgta tttatcacag gcttaactgt tatgcatagc ataaacatcc gttttgagct
     5101 ggcaattgtt tgtcttattg gtattttgct ttatttgaat ggatacaaaa aaacactttt
     5161 caaatggatt atattgtgtg ggatacttta ctccttgcct aattttatgg tgttatctga
     5221 attaaaccct atagttaaga tgtttttgag tatacctatt gttattagaa tgtttatttt
     5281 accatttatg gcagcaagct ttatgataaa gacctcggat gtaggcgcaa taatttcatc
     5341 gatggataag cttaagattt caaagaatgt atccatacct attgcggtta tgtttagatt
     5401 cttcccatct tttaaggagg agaagaaaaa catcaaaatg gctatgagag taagagggat
     5461 aaattttaaa aacccagtca aatatcttga atatgtttct gtgccactac tcattatatc
     5521 atctaatata tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag aaaatccaat
     5581 tgccaagacc agatacattc gcgtaaagat acagctaatt gattttgttt atgttttagc
     5641 ggttgttgga cttattgtgg gaggcttaat atggttgaaa taaaaaattt aagtcttgat
     5701 tatggtgaag agcatatatt agatgatata tcactatcca tagccgaggg agagtgcgtg
     5761 ctatttacag gaaaaagtgg aaatggtaag tcatctttaa taaattcaat caatggacta
     5821 gctgtaaggt atgataacgc aaagacaaag gtcgaaataa ttattgatgg taagaatata
     5881 aaaaatttgg aactttatca aatctcaatg cttgtttcaa ctgtttttca aaatcctaag
     5941 acatattttt ttaatgtcaa tacgacatta gaattattat tttatttgga aaatatcggt
     6001 cttgcaagag aagagatgga caggcgtttg aaggatatgc ttgagatatt cccgataaaa
     6061 aatcttttga acagaaatat atttaatcta tccggcggtg aaaaacaaat tctttgcatt
     6121 gcagcttctt atatagcagg tacaaagatt atagttatgg atgagccttc atcgaattta
     6181 gatattaaaa gcataagtgt tttggcaaag atgctaaaga tattaaaaga gaaaggcata
     6241 agcataattg ttgcagagca tagaatttat tatttgatgg acatagttga ccgtgtattt
     6301 ttaatagata aaggaaagct taaaaaaact tatactagaa gtgaattttt aaagctagat
     6361 aaaaatgaat taaatgcttt aagtttaaga gataaagaat taagtaaatt aaaagttcct
     6421 tatttaaaag aaggtggaga gtatcagata aaaaatctta gctacaaatt tactgatgat
     6481 gagtgtttaa gcttaaaaga tatttcgttc aagcttggga aaatttatgg cataatagga
     6541 tccaacggac gaggaaaatc aacgctttta agatgtttaa taggtcttga gaaaaaatca
     6601 aaagaagaaa tttattttaa gggagagaag ctatctaaaa aagaaagact caaaaactct
     6661 tcacttgtta tgcaagatgt aaatcatcaa ttattcacag atgaagtatt caacgagctt
     6721 agattaggag taaagaattt tgatgaagaa aaggcgaaaa tcattttaaa agatttaggc
     6781 ctggacgaat ttattgaaag gcatccgatg agtttatcag gagggcaaaa gcaaaggctt
     6841 gcaatagcat ctgttatgtg caagaattct ccatttgtct tttttgacga accttcaagt
     6901 ggtatggatt attccaatat gataaaaata tctgaactga ttaataagta taaaaccatg
     6961 gataaaataa tttttattgt ttcccatgat atagaatttt taaatgaagt ggcagatgaa
     7021 atttttgaat tgtaaaatag agtctgttaa acat
//
LOCUS       DBERCA010000073         6808 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.073, whole genome shotgun sequence.
ACCESSION   DBERCA010000073 DBERCA010000000
VERSION     DBERCA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.073"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..376
                     /locus_tag="ACL719_001806"
     CDS             <1..376
                     /locus_tag="ACL719_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS0699725.1"
                     /translation="GKVSGRRYQRISLVAGLTNGELIVPMTYEEMMTSDFFEVWFQKF
                     FLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKH
                     LKKVLPSCNTFYEAFLSCSCFN"
     gene            437..1399
                     /locus_tag="ACL719_001807"
     CDS             437..1399
                     /locus_tag="ACL719_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS0699726.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1576..2583
                     /gene="gap"
                     /locus_tag="ACL719_001808"
     CDS             1576..2583
                     /gene="gap"
                     /locus_tag="ACL719_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGS0699727.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2651..3526)
                     /locus_tag="ACL719_001809"
     CDS             complement(2651..3526)
                     /locus_tag="ACL719_001809"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0699728.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            3613..5808
                     /gene="pbp2a"
                     /locus_tag="ACL719_001810"
     CDS             3613..5808
                     /gene="pbp2a"
                     /locus_tag="ACL719_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGS0699729.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5861..6013
                     /gene="rpmG"
                     /locus_tag="ACL719_001811"
     CDS             5861..6013
                     /gene="rpmG"
                     /locus_tag="ACL719_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0699730.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6023..6199
                     /gene="secE"
                     /locus_tag="ACL719_001812"
     CDS             6023..6199
                     /gene="secE"
                     /locus_tag="ACL719_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGS0699731.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6254..6790
                     /gene="nusG"
                     /locus_tag="ACL719_001813"
     CDS             6254..6790
                     /gene="nusG"
                     /locus_tag="ACL719_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGS0699732.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2118 a         1188 c         1474 g         2028 t
ORIGIN      
        1 aggcaaagta tctggaagaa gatatcagag gatttctttg gttgcaggtc taacaaatgg
       61 tgagttaatc gttccaatga cttacgaaga gatgatgacg agcgactttt ttgaagtatg
      121 gtttcagaag tttttcttac caacattaac cacaccatcg gttattatta tggataatgc
      181 aagattccat agaatgggta agctagaact cttgtgtgaa gagtttgggc ataaactttt
      241 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg ctcatatcaa
      301 aaagcacctc aaaaaggtat taccaagttg caataccttt tacgaggctt ttttatcctg
      361 ctcttgtttc aattgactat agttcacgga tacagttggg aaagaagcta ttaaatattt
      421 ggagagtcct tttgggatga acatgtcaaa tattactata gatgggcaag aaatggatct
      481 gagtcaaacg atgccttctt atgctcaagt agtgagtctt tatgatgata caagtatctc
      541 tgttagtaat aatgagacgg acaaagtgtt ggctggatcc ctctatactg atacaaatga
      601 acagggatta acgattccaa gcagtttact aaaaaattgg aatgaacaga caggaaaaaa
      661 tttgacagct aatgatctta ttggcaaatc agtctcagcc agcattgtag aaagtgctgc
      721 cgaaactagt aagattgctc aatttcaaac gaagattgta cgtgtaatca atgatgaaga
      781 tgacatggag gacagcaaca gtttcatgct gtctcatcaa atggaaacga ttttgaaaga
      841 ggctggattt acgaaagctg tatcttattt tatcttggaa ctcaaagatc catcacagac
      901 aaaagtagta acagaagaat tacagaaaaa taagaagtat actgtgcttt ctcaacagag
      961 agttcttgat attgtgatta cctttattcg tgttattcag ggattattga ttgtgctttc
     1021 atcacaagct attgtggtag cagcggttat gattggtatc attatttaca tcaatatcat
     1081 gcaacgttcc aaggaaatag gtgtcatgaa agcagttggt tatcagaatc gtgatgtcaa
     1141 aggaattttt atttacgagg ctatctggat tgtaggcatc gccttgctgc tggcattttt
     1201 ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta agtcactttt acccatccat
     1261 cactaaggtt tttgaattaa atcttttatc tgttttagga actctagttt tcgctctatt
     1321 acttggttat gtctcagcct acttcccggc gcgtaagatt agtaaaatgg atcctataga
     1381 atcgttatgc tatgaatagt aagggattcc ttggtttaca gtcttaaaaa gaaactaaca
     1441 tagaaaaaca atggccatac ttgcaattca actcaaatag ttgtataata ggtttgttga
     1501 aaggtgattt gtagtgattc aagttactct tttcacaata aaaatttcat gattttcata
     1561 aggaggaaat cactaatggt agttaaagtt ggtattaacg gtttcggacg tatcggtcgt
     1621 cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag ttacacgcat caacgacctt
     1681 acagatccag ttatgcttgc acacttgttg aaatacgaca caactcaagg tcgtttcgac
     1741 ggtactgttg aagttaaaga aggtggattt gaagttaacg gtaaattcat caaagtttct
     1801 gctgaacgtg atccagaaca aatcgactgg gctactgacg gtgtagaaat cgttcttgaa
     1861 gctactggtt tctttgctaa gaaagaagca gctgaaaaac accttaaagg tggagctaaa
     1921 aaagttgtta tcactgctcc tggtggaaac gacgttaaaa cagttgtatt caacactaac
     1981 cacgacgttc ttgacggtac tgaaacagtt atctcaggtg cttcatgtac tacaaactgc
     2041 ttggctccaa tggctaaagc tcttcaagac aactttggtg ttgttgaagg attgatgact
     2101 actatccacg cttacactgg tgaccaaatg atccttgacg gaccacaccg tggtggtgac
     2161 cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta actcaactgg tgctgcaaaa
     2221 gctatcggtc ttgtaatccc agaattgaat ggtaaacttg acggatctgc acaacgcgtt
     2281 ccaactccaa ctggatcagt tactgaattg gtagcagttc ttgaaaagaa cgttactgtt
     2341 gatgaagtga acgcagctat gaaagcagct tcaaacgaat catacggtta cacagaagat
     2401 ccaatcgtat cttcagatat cgtaggtatg tcttacggtt cattgtttga cgcaactcaa
     2461 actaaagttc ttgacgttga cggtaaacaa ttggttaaag ttgtatcatg gtacgacaac
     2521 gaaatgtcat acactgcaca acttgttcgt actcttgaat acttcgcaaa gattgctaaa
     2581 taattcttga gttgatagaa agcaaggctt tgtggtcttg cttttttata tggaaaaatg
     2641 gatgacacga tcatccattc ttttttaatt ctttttcaaa tgtatttgaa agggtagtga
     2701 aagttagcct ctctaaagta agtgggtggg taaaggaaag tcggaaggca tgaagcataa
     2761 gccggcttgt ctttgattta ctattataga gagggtctcc caggatagga agattatgat
     2821 gcaaaaggtg cacacgaatc tgatgggttc gccctgtctt tagcttgcaa tgagccaagg
     2881 aagtcttgtt tgagaattgc tttaatctgc ttacatgcgt ttcagcatat tgcccatttt
     2941 ttgcatcaac tattcttttt ctacgatcat ggcgatcacg tccaattttg tctctgaaaa
     3001 caagttcttt tctgttgata tttccatcaa ctagagccca atattctcta gaaatctctt
     3061 ttttctccaa taagcgattg agaatgggca ggataaaagg atttttggca aagagaacta
     3121 agccactggt ttccatgtcc agacgatgaa cgacatagca ggtttggcca acataggtac
     3181 tgacatggtt aagaagggca atttcgtttg gttgattacc atgcgttttc atcccctctg
     3241 gtttgtttac aataatcaag tgttgatctt gataaacttc ctgcactaag tctgggttgc
     3301 cccaagggat cgtcttttgg gaataatctt cctcgtcaaa agtcaactgg caaacatctc
     3361 caggatttac gatttcgttc cagtggactt cttcttgatt tatcaaaata tgtttcttga
     3421 ttctcaaaaa atgacggatt tttctaggga tgaggagttg ttcctcaagt aattgcttta
     3481 ccgtcatttg aggtagagag gcgggtaatg taaatgtgaa ttgcatacag atattgtaac
     3541 aaaaaaagcc ctatttggat aggaaatagc taaattcttg tcttcctatg atgaagatga
     3601 taaaataaac gcatgaaatt agataaatta tttgagaaat ttctttctct ttttaaaaaa
     3661 gaaacaagtg aactagagga ctctgattct actatcttac gtcgctctcg tagtgatcga
     3721 aaaaaattag cccaagtagg tccgattcga aaattctggc gtcgttatca tctaacaaag
     3781 attatcctta tactaggttt gagtgcagga ttgctagttg gaatctattt gtttgctgta
     3841 gccaagtcga ccaatgtcaa tgatttgcaa aatgccttga aaactcggac tcttattttt
     3901 gaccgtgaag aaaaagaggc tggtgccttg tctggtcaaa agggaaccta tgttgagctg
     3961 actgacatca gtaaaaactt gcagaatgct gttattgcga cagaagaccg ttctttctat
     4021 aaaaatgacg ggattaacta tggccgtttc ttcttggcta ttgtcactgc tggacgttca
     4081 ggtggtggct ctaccattac ccaacagctg gctaaaaacg cctatttatc gcaggatcaa
     4141 actgttgaga gaaaagcgaa agaatttttc cttgccttag aattaagcaa aaaatatagt
     4201 aaggagcaaa ttctaaccat gtaccttaac aacgcttatt ttggaaatgg tgtgtggggt
     4261 gtagaagatg cgagtaagaa atactttgga gtttctgcat cagaagtgag tctggatcaa
     4321 gctgcgactc tggcagggat gctcaagggg ccggaactgt ataatccctt gaattccgta
     4381 gaagattcta ctaatcggcg cgatactgtc ttgcagaata tggttgcagc aggatatatt
     4441 gataaaaacc aagaaaccga agctgctgaa gttgatatga cttcgcaatt gcacgataag
     4501 tatgaaggaa aaatctcaga ttaccgttac ccctcttatt ttgatgcggt ggttaatgaa
     4561 gctgtttcca agtataatct aacagaggaa gagattgtca ataatggcta ccgcatttac
     4621 acagagctgg accaaaacta ccaagcaaat atgcagattg tttatgaaaa cacatcgcta
     4681 tttccgaggg cagaggatgg aacgtttgct caatcaggaa gtgtagctct cgaaccgaaa
     4741 acagggggag ttcgtggagt tgtcggtcaa gttgctgaca atgataaaac tggattccgg
     4801 aatttcaact atgcaaccca atcaaagcgt agtcctggtt ctacaattaa gcctttagtt
     4861 gtttatacgc cagcagttga agcaggctgg gctttgaata agcagttgga taaccatacc
     4921 atgcagtatg acagctataa ggttgataac tatgcaggga tcaaaacgag tcgagaagtt
     4981 cctatgtatc aagccttggc agaatcgctt aatctacctg ctgttgccac tgttaatgat
     5041 ttgggtgtcg acaaggcttt tgaggcaggc gaaaaattcg gactcaacat ggaaaaggtc
     5101 gaccgtgttc ttggtgtcgc cttgggaagc ggtgttgaaa ccaaccctct tcaaatggct
     5161 caagcatacg ctgcctttgc aaatgaaggt ttaatgcctg aagctcattt tattagtaga
     5221 attgaaaatg ctagtggaca agttattgcg agtcataaaa attcacaaaa acgggtgatt
     5281 gataagtctg tagctgacaa gatgaccagt atgatgttgg ggactttcac caacggtacc
     5341 ggtattagtt catcgcctgc agactatatt atggcaggga aaactggaac aactgaagca
     5401 gtttttaatc cggagtacac aagtgaccaa tgggtaattg gttatactcc ggatgtagtg
     5461 attagccact ggcttggctt tccgaccact gatgaaaatc actatctagc tggctctact
     5521 tcaaacggtg cagctcatgt ctttagaaac attgccaata ccattttacc ttatacgcca
     5581 ggaagtacct ttacggttga aaatgcttat aagcaaaatg gaattgcacc agccaataca
     5641 aaaagacaag tacaaaccaa tgataatagc cagacagatg ataatttgtc tgatattcga
     5701 gggcgtgcgc aaagtctagt agatgaggct agccgggcta tctcagatgc gaagattaag
     5761 gaaaaggctc aaacaatatg ggattcgata gtcaatctat ttcgctaaga tgcttgtcaa
     5821 agcctagctt tcttgttata atggataaga tggaggcgtt atggcactaa aaaaagcaag
     5881 cctagcttgt gcggtttgtg gttcgagaaa ctattcaatc aagatcagcg gaaaccccaa
     5941 gcctacacga ctagaagtaa ataaattttg taagcattgt ggcaagtaca ctacacacag
     6001 agaaacgaga taggagagag cgatgcgttt tattggagat atttttagac ttcttaaaga
     6061 cacaacatgg ccaactcgca aggaaagctg gagagatttt cgttctatca tggaatacac
     6121 agctttcttt gtagtaatta tttacatttt tgaccagttg attgtttcag gtttgattcg
     6181 atttattaac attttttaga agattagtgg agttaattac actagaaatc ttctatttat
     6241 gaaaggaaat atcatggata gttttgataa aggatggttt gttttacaaa cttattctgg
     6301 ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca caaacctaca atatgttgga
     6361 taatatttta cgcgttgaaa ttccaacaca aacagtgcaa gttgaaaaaa atggaaagag
     6421 aaaagaagta gaagaaaatc gctttccagg ttatgttctt gtagaaatgg tcatgacaga
     6481 tgaagcttgg tttgttgttc gaaacacacc aaatgttaca ggatttgtcg gatcacacgg
     6541 gaacagatca aaaccaactc cattattgga acaagaaatt cgtgacattt tggtatctat
     6601 gggacaaact gttcaagaat ttgatttcga tgttgagatt ggtcaaactg tacgtattat
     6661 tgatggtgct tttgcagact acactggtaa gattacagaa attgataata acaaagtgaa
     6721 aatgattatc tctatgtttg gtaatgacac agttgcagaa gtaaacctaa accaaatcgc
     6781 agaattataa ccctgagaga ggctggag
//
LOCUS       DBERCA010000074         6634 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.074, whole genome shotgun sequence.
ACCESSION   DBERCA010000074 DBERCA010000000
VERSION     DBERCA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.074"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            51..914
                     /locus_tag="ACL719_001814"
     CDS             51..914
                     /locus_tag="ACL719_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0699733.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYIYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1297..1917
                     /locus_tag="ACL719_001815"
     CDS             1297..1917
                     /locus_tag="ACL719_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699734.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2003..2446
                     /gene="tsaE"
                     /locus_tag="ACL719_001816"
     CDS             2003..2446
                     /gene="tsaE"
                     /locus_tag="ACL719_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGS0699735.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2436..2954
                     /locus_tag="ACL719_001817"
     CDS             2436..2954
                     /locus_tag="ACL719_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS0699736.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2962..3978
                     /locus_tag="ACL719_001818"
     CDS             2962..3978
                     /locus_tag="ACL719_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGS0699737.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNLLRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4063..5319
                     /locus_tag="ACL719_001819"
     CDS             4063..5319
                     /locus_tag="ACL719_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGS0699738.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGNPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHVFNLVRKALLSD"
     gene            5374..6540
                     /gene="recA"
                     /locus_tag="ACL719_001820"
     CDS             5374..6540
                     /gene="recA"
                     /locus_tag="ACL719_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGS0699739.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2114 a         1081 c         1493 g         1946 t
ORIGIN      
        1 ctaaaaactt tataatgaaa ttaagcaaat aggaaggttt attatcttta atgaatacac
       61 tcgcagagaa attcagattg aaaagaaaag agttgagact ctcccaacaa actcttgcag
      121 aaggaatttg tgaacaaagc cagattagta aaattgaaag aggtcatttt attccctccg
      181 cagacttgtt attcaaactc tctcaacgac ttgaagtccc tttggattac tttttcaatg
      241 aacaaatcga agttaagtct aacctctcta atttcaaaca tctatctgct cgcctattag
      301 atgacagaaa ttatgacgat ttagaatata tttataaaat agaggtagaa cgaagcactt
      361 ttctaacatt agaagaccga acttaccttg aatggattaa atctataatt gacttctatc
      421 aatatgacag taagtgtgag gcaatttcgt cattggaaaa tatattatta aaagtctctt
      481 caaatactct gatttattta aaggccttga atactctatc taatttctat tccttagtgg
      541 gtcgtgaaca agaatatgag gcaaactact ctcatttaat agagttgtat cagacaaaaa
      601 atttggacca tcaagagttt ttatttggct acatcagagt tcgttacaac tacgctcact
      661 acctagtatc aaaggaaaaa tataatgaag ccatccaaga agctcttgaa acgattgaac
      721 tctgtaaaca aagacagaca agctaccaat tggctcctct acttattctt gtaggaaatg
      781 ctggagccaa atttctagac agagaacaag ttaaaaatta ttatatagaa gcaagagagt
      841 tatgtaagat ttataacaat cctttaatgt tgatgaagat agaaaattat ttgaaggaac
      901 tagatactgt ttagttaatc ttgacattat agtttttctg aaacgttaaa taacctgtaa
      961 ggctgatagt tagattaata ctatcagcct tataattatg tgactctgag tccgtctcgc
     1021 tccgtgaggt gcgagacaaa acaaccaccc gtttgacgga ttttcatgat ttcaaattta
     1081 tattcttcga aaatctcttc aaatcacgtc agctctatct gcaatctcaa aacagtgttt
     1141 tgagcaacct gtggctagct acctagtttg atctttgatt ttcattgagt attagtctct
     1201 agtgtttgtt tagcgtctta aagagacatt tgagaagatt agaaacagtt ctctcttttc
     1261 tcctagtata atgattggta ggaagggaga gagaagatga aatcaatgag aatcttattt
     1321 ttgttagctt taattcaaat cagtttgagt agctgtttct tatggaagga atgcatcttg
     1381 tcctttaaac aaagtacagc ttttttcatc ggaagcatgg ttttcgtttc aggaatctgt
     1441 gctggagtaa attatcttta tacccgtaag caagaagtcc atagtgtcct agccagtaag
     1501 aagtcggtga agctttttta cagtatgtta ctcttaatta atttgttagg agctgttctt
     1561 gttttgtcag ataacttgtt catcaaaaat acgctgcagc aagaattagt tgacttttta
     1621 ttgccatcct tctttttcct atttgggcta gatttgctga tttttttacc cttgaaaaaa
     1681 tacgtgcgcg attttcttgc tatgctagac agaaaaaaga cagtgttggt gactatttta
     1741 gcaacacttc ttttcttaag aaatccaatg accattgtct cacttctgat ttatattgga
     1801 ctgggcttgt tttttgcagc ctatcttgtc ccaaattcgg ttaagaagga agtttccttt
     1861 tatggtcata ttttccgaga tcttgtattg gtcattgtta cgctcatttt cttttagaga
     1921 aagcctgttt tttatggatg ttatgatgat aagtttagta ggaaaataga cacaaaagaa
     1981 aagtttttgg tataataaga gtatgtacat aaaaaatgaa gaagagttgc aagccttagg
     2041 ggagcgtttg ggccatctat tagcaaagaa tgatgtttta atcttaattg gagaactggg
     2101 tgcaggtaaa acgaccttta ctaaaggact tgcaaaagga ttacagattt ctcaaatgat
     2161 taaaagtccc acctatacta tcgtgagaga gtatgaaggt cgacttccac tttatcacct
     2221 agatgtttat cgtattgaag gagatgctga ttctatcgac ttggatgagt ttatctttgg
     2281 tggtggcgtg actgttattg agtggggaaa tctcttagga gatgccttgc cagatgctta
     2341 tttagaattg gaaattctaa aagaagcaga tggacgccgt ttaaattttc aggcaaaggg
     2401 tttgcgtgct gagaaattgt tagaggagct tcaatatgga gtatgaattg ctcattaggg
     2461 aagcagagcc caaagatgca gctgaattag tggccttttt aaatcgtgtg agtttggaga
     2521 cagactttac cagcctagac ggagatggta ttctcttgac tagtgaggag atggaaatat
     2581 tcctcaacaa gcaagctagt tcggacaatc agataacctt acttgcattc ttaaatggca
     2641 aaattgctgg tattgtaaat attacagctg accagcgtaa gagagtccgt catattggag
     2701 atctcttcat tgtgattgga aaaagatatt ggaataatgg cttgggaagt ttgttgctag
     2761 aagaagcgat agagtgggca caagcaagtg gcattctgcg tcgtctccaa ctgactgtcc
     2821 aaactcgtaa tcaagcagca gtccatcttt atcaaaagca tggctttgtc attgaaggta
     2881 gccaagagcg tggggcatat atagaagaag ggaaatttat cgatgtttac ttgatgggta
     2941 aactgatagg ttagtagaaa tatggttaaa aaaattattg gaatggtgct agctttactt
     3001 tctgtaactg tagtaggagt aggtgttttt gcttatacta tttatcaaca agggacagaa
     3061 accttagcta aaacctataa aaaaatcggc gaagaaacca aggttattga agcgactgaa
     3121 cctctaacca ttctgttaat gggagtggac accggaaatg ttgaacgaac tgaaacttgg
     3181 gtcggtagaa gtgatagcat gatcttgatg acagtgaatc ctaaaacgaa aaaaacaaca
     3241 atgatgagtt tagagcggga tattctgacg cgcattgaat cagggaatgg tcaggctcat
     3301 gaagcgaaac tgaactcagc atatgcagat ggtggagcag agcttgctat agaaaccatt
     3361 caaaaaatga tgaatatcca tattgatcgc tatgtgatgg tcaatatgag aggattgcaa
     3421 aaactagtgg atgcagtagg aggtattaca gtcaataata tcctaggttt cccaatttct
     3481 atcagtgacc aagaagaatt taatactatt tctatcggtg ttggggagca acatattggg
     3541 ggagaagaag ccctagtcta tgcacgaatg cgttaccaag atcctgaggg ggattatggt
     3601 cgtcaaaaac gtcaacgtga agttattcaa aaagtcatgg aaaaagctct cagtttaaat
     3661 agcattggtc attatcaaga gattctaaaa gctttgagtg acaatatgca gaccaatatt
     3721 gatttgtctg caaaaagtat ccctaacttg ctaggctata aagattcatt taaaaccatt
     3781 gaaactcagc agttgcaggg tgaaggagag atacttcaag gtgtttctta ccagattgtt
     3841 tcgagagcac atatgttgga aatgcaaaat ctactccgac gttctttggg acaagaagaa
     3901 gttactcagc ttgaaaccaa tgcggtttta tttgaagatt tatttggcag agcacctgtt
     3961 ggtgatgaag ataattaact tttgatacaa ataaaaaaat caatcgtagg aaaattcctg
     4021 cgattttttc aaaaaaatac gaatagatag gtaggaggaa acatgaaagc agaaatcatt
     4081 gctgttggaa cagagatttt gacagggcag attgtcaaca ccaatgccca gtttttgtcg
     4141 gaaaaactag ctgagattgg ggtagatgta tattttcaga cggctgtagg agacaatgaa
     4201 gttcgtctct tgtctttgct tgagattgcc agtcaacgta gcagtctggt gattttgaca
     4261 ggcggtttgg ggccaactga ggacgaccta accaaacaaa ccctagctaa atttttaggg
     4321 aaagcattag tctttgatcc tcaggctcag gagaagttgg atatcttttt taccctgcga
     4381 ccagactatg cccgaacacc gaataacgaa agacaagctc aaattgtaga aggagcgatt
     4441 ccactgccaa acgaaacagg actggctgtt ggaggaaaat tagaagtaga cggagtgacc
     4501 tatgtcgtcc ttccaggtcc gccaagtgaa ttgaaaccca tggtcttaaa ccaacttcta
     4561 cccaagttga tgacagggag caagctgtat tcccgagttc ttcgtttctt tgggattggc
     4621 gagagccagt tggttacgat tttggctgat ttaattgata atcagatcga tcctaccttg
     4681 gccccttatg ccaagacagg agaagtcact ctacgtctgt caacaaaggc tagcagtcaa
     4741 gaagaggcga atcaagcgct ggatatcttg gaaaatcaaa tcttggactg ccagactttc
     4801 gaaggaattt ctttacgaga cttttgctat ggttatgggg aagaaactag tttagccagc
     4861 attgtggtag aagaactgaa aaggcaaggg aaaaccatcg cggctgcaga gagtttgacg
     4921 gcaggtcttt tccaagctac cgtggcgaat ttttctggag tttcaagtat atttaagggc
     4981 ggttttgtga cctatagctt ggaggaaaaa tcaaggatgt tggatattcc tgccaagaat
     5041 ttggaagaac atggtgtggt gtctgaattt acagctcaga agatggctga gcaggcacga
     5101 agcaagaccc agtctgattt tggaattagt ttgactggag tggcaggacc agatagccta
     5161 gaaggaaacc cagttgggac agtcttcata ggcttggcgc aagatcaagg aactgaggtt
     5221 atcaaggtta atattggagg cagaagccga gcagatgtac gtcacattgc ggttatgcat
     5281 gtctttaacc tagttcgcaa ggctttatta agtgactaac ttttgatata atagtagata
     5341 ggtctgagga tcattagaat gtaggagaat agaatggcga aaaaaccaaa aaaattagaa
     5401 gaaatttcaa aaaaatttgg ggcagaacgt gaaaaggcct tgaatgacgc tcttaaattg
     5461 attgagaaag actttggtaa aggatcaatc atgcgtttgg gtgaacgtgc ggagcaaaag
     5521 gtgcaagtga tgagctcagg ttctttagct cttgacattg cccttggctc aggtggttat
     5581 cctaagggac gtatcatcga aatctatggc ccagagtcat ctggtaagac aacggttgcc
     5641 cttcatgcag ttgcacaagc gcaaaaagaa ggtgggattg ctgcctttat cgatgcggaa
     5701 catgcccttg atccagctta tgctgcggcc cttggtgtca atattgacga attgctcttg
     5761 tctcaaccag actcaggaga gcaaggtctt gagattgcgg gaaaattgat tgactcaggt
     5821 gcagttgatc ttgtcgtagt cgactcagtt gctgcccttg ttcctcgtgc ggaaattgat
     5881 ggagatatcg gagatagcca tgttggtttg caggctcgta tgatgagcca ggccatgcgt
     5941 aaacttggcg cctctatcaa taaaaccaaa acaattgcca tttttatcaa ccaattgcgt
     6001 gaaaaagttg gagtgatgtt tggaaatcca gaaacaacac cgggcggacg tgctttgaaa
     6061 ttctatgctt cagtccgctt ggatgttcgt ggtaatacac aaattaaggg aactggtgac
     6121 caaaaagaaa ccaatgtcgg taaagaaact aagattaagg ttgtaaaaaa taaggtagct
     6181 ccaccgttta aggaagccgt agttgaaatt atgtacggag aaggaatttc taagactggt
     6241 gagcttttga agattgcaag cgatttggat attatcaaaa aagcaggggc ttggtattct
     6301 tacaaagatg aaaaaattgg gcaaggttct gagaatgcta agaaatactt ggcagagcac
     6361 ccagaaatct ttgatgaaat tgataagcaa gtccgttcta aatttggctt gattgatgga
     6421 gaagaagttt cagaacaaga tactgaaaac aaaaaagatg agccaaagaa agaagaagca
     6481 gtgaatgaag aagttacgct tgacttaggc gatgaacttg aaatcgaaat tgaagaataa
     6541 gctgttaaag cagtggagaa atccgctact ttttcgattt ttgattcagg tttttagatt
     6601 atatatagta gcttgaaata agatatgaac aact
//
LOCUS       DBERCA010000075         6248 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.075, whole genome shotgun sequence.
ACCESSION   DBERCA010000075 DBERCA010000000
VERSION     DBERCA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.075"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            170..370
                     /locus_tag="ACL719_001821"
     CDS             170..370
                     /locus_tag="ACL719_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699740.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATQAVLLDAQKAPEDSALTE"
     gene            395..811
                     /locus_tag="ACL719_001822"
     CDS             395..811
                     /locus_tag="ACL719_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HGS0699741.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1042..1749
                     /gene="ccdA2"
                     /locus_tag="ACL719_001823"
     CDS             1042..1749
                     /gene="ccdA2"
                     /locus_tag="ACL719_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS0699742.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLTLASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1760..2317
                     /gene="sdbB"
                     /locus_tag="ACL719_001824"
     CDS             1760..2317
                     /gene="sdbB"
                     /locus_tag="ACL719_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGS0699743.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2691..4082
                     /locus_tag="ACL719_001825"
     CDS             2691..4082
                     /locus_tag="ACL719_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGS0699744.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4225..5142
                     /gene="adcAII"
                     /locus_tag="ACL719_001826"
     CDS             4225..5142
                     /gene="adcAII"
                     /locus_tag="ACL719_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGS0699745.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5150..>6248
                     /gene="phtB"
                     /locus_tag="ACL719_001827"
     CDS             5150..>6248
                     /gene="phtB"
                     /locus_tag="ACL719_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HGS0699746.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPS"
BASE COUNT         1905 a         1150 c         1318 g         1875 t
ORIGIN      
        1 gtaaaatgaa ataagaacag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagc ctatatgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc
      121 tgcaagtcct gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag
      181 cgcttcggct agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccaaa
      301 aatcgatgca acccaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa
      421 agatgtgaaa gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca
      481 aggaacggaa gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg
      541 agtgattgtg gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg
      601 taagaaagag tcagaaatcc agcaattaag cacggaattg atcaaggttc taggacagct
      661 agatgcagaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac
      721 ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga
      781 gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacctaaa
      841 agacattttc gttcacaaaa gcagtgacga gtaatccttg tcgttgcttt ttggctattt
      901 tgggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac
      961 gattttgcgt agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt
     1021 attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca
     1081 ggtgttttat cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt
     1141 ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg
     1201 tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta
     1261 ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg atatgtcatg
     1321 ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta
     1381 gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt
     1441 ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct
     1501 gttttagcac ttgcggcttc tggaggcaat ggtgcttggc aaggagcgat ttatactctc
     1561 atttacactc tgggcatggc ccttcctttc ttggtcttga cactagcttc aggtctagtc
     1621 atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc
     1681 ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt
     1741 tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt
     1801 agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc
     1861 aaaacaacaa acgactgtac aacaaatttc tgttggaaaa gatgctccag actttacatt
     1921 gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt
     1981 gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact
     2041 agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg
     2101 tgaaaaaact gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc
     2161 agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac
     2221 agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa
     2281 tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag
     2341 atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa cttagctttt
     2401 agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt
     2461 tctttttctt gatatataag ggttcaaaag cgaaaagact cagaaaaagt gcacgacaaa
     2521 tagccctaaa acagagtcgt cttagagtaa cttccagttt tgggagagag atggaagtta
     2581 ctttgagaag ttacggtagt cagatttttt tagaaaaaca ttttataaat attcacatat
     2641 ctcctatatt tatggtaaaa tagaattatc agtttatttt ggagtcaaag atgaatatat
     2701 ttagaacaaa gaatgttagt ttagataaaa cagagatgca taggcatttg aagttatggg
     2761 atttgatttt gctgggtatc ggagccatgg tagggacagg cgtctttaca atcacaggta
     2821 ctgcagctgc aacacttgct ggcccagccc tagtgatttc aatcgttatt tctgccttgt
     2881 gtgtgggatt atcagccctc ttttttgcag aatttgcctc gcgagtaccc gctacaggag
     2941 gtgcctatag ttacctctat gctatcttag gagaattccc tgcctggttg gctggttggt
     3001 taaccatgat ggagttcatg acagccatat caggcgtagc ttcgggttgg gcagcttatt
     3061 ttaaaggctt gctctctcaa tatgggatag cccttcctca ggctttaaat ggtaccttta
     3121 atcctcaagc agggacattt gttgatttat tgcctattct tgtcttggtc ttggtgactt
     3181 cgcttgtttt actaaatgcc aaggcagcct tacgctttaa ttcgattcta gttattttga
     3241 aattttccgc tttagctctc tttgttttag taggaatttg gaatattaag tttgataatt
     3301 ggagcaattt tgctccttac ggctttggac aaatctatgg agctagtact ggtattatgg
     3361 ctggtgcgtc cttgatgttc ttcggttttt tgggatttga atccatctct atggctgtag
     3421 atgaagtcaa gactcctcaa aaaaatattc ctagagggat tgtcttatcg ctttctatcg
     3481 taaccattct ctatgccttg gtgacccttg ttttgactgg tgtggttcac tatagtcatt
     3541 taaatgtcga tgatgccgtt gcctttgccc ttcgtagtgt tgggattagt tgggcagcca
     3601 actatgtgtc attagtggct atcttgacct tgattacagt ttgtatttcg atgacctatg
     3661 ccctatcgcg tatgatttac agtttagcac gtgacggctt agtgcctgct gcctttaagg
     3721 aactgacgaa gactagcaag gtaccaaaga atgctactat cttgacaggt ctagcttcag
     3781 cagtagcagc aggaatgttc ccactagcca gtattgcagc ctttttaaat atttgtacct
     3841 tagcctactt gatcatgctg gcttatggtc tgattcgctt acggaaagaa aaaggaatgc
     3901 ccaaagctgg ggaatttaaa acaccattgg tacctctgtt accaatttta tcgatcatca
     3961 tttgtttatc cttcatgtta cagtataata tgaatacctg gattgctttc ctagttgcgt
     4021 tgttagtagg aagtattatt tacttcactt atggctataa gcattctacc atagaagaat
     4081 aaagtgaaag tctattgagt tgttgacact cagcagattt ttatatgtga aagaaatttc
     4141 aaattttttc taaaaatatc ttgacagatt aaattttcag gagtagaata tttactagtt
     4201 aattaaatgg ttaaggagtt gttcatgaag aaacaaaatt tatttttagt cctgttaagt
     4261 gtctttcttt tgtgcttggg ggcttgtggt caaaaggaaa gtcagacagg aaaggggatg
     4321 aaaattgtga ccagttttta tcctatctac gctatggtta aggaagtatc tggtgacttg
     4381 aatgatgttc ggatgattca gtcaagtagt ggtattcact cctttgaacc ttcggcaaat
     4441 gatatcgcag ccatctatga tgcagatgtc tttgtttacc attctcatac actcgaatct
     4501 tgggcaggaa gtctggatcc aaatctaaaa aaatccaaag tgaaggtctt agaggcttct
     4561 gagggaatga ccttggaacg tgtccctgga ctagaggatg tggaagcagg ggatggagtt
     4621 gatgaaaaaa cgctctatga ccctcacaca tggctagatc ctgaaaaagc tggagaagaa
     4681 gcccaaatta tcgctgataa actttcagag gtggatagtg agcataaaga gacttatcaa
     4741 aaaaatgcgc aagcctttat caaaaaagct caggaattga ctaagaaatt ccaaccaaaa
     4801 tttgaaaaag cgactcagaa aacatttgta acacaacata cagccttttc ttatctagcg
     4861 aagagatttg ggcttaatca acttggtatt gcaggtatct ctcctgaaca agaaccaagt
     4921 ccacgacaac taacagaaat tcaggaattt gttaagacct ataaggttaa aacgattttt
     4981 acagaaagta acgcttcttc aaaagtagct gaaactcttg tcaaatcaac aggtgtgggt
     5041 cttaaaactc tgaatccttt agagtcagac ccacaaaatg acaagaccta tttagaaaat
     5101 cttgaagaaa atatgagtat tctagcagaa gaattaaagt gaggaaagaa tgaaaatcaa
     5161 taaaaaatat ctagcaggtt cagtggcagt ccttgcccta agtgtttgtt cctatgaact
     5221 tggtcgtcac caagctggtc aggttaagaa agagtctaat cgagtttctt atatagatgg
     5281 tgatcaggct ggtcaaaagg cagaaaattt gacaccagat gaagtcagta agagagaggg
     5341 gatcaacgcc gaacaaattg ttatcaagat tacggatcaa ggttatgtga cctctcatgg
     5401 agaccattat cattactata atggcaaggt tccttatgat gccatcatca gtgaagaact
     5461 tctcatgaaa gatccgaatt atcagttgaa ggattcagac attgtcaatg aaatcaaggg
     5521 tggctatgtg attaaggtag acggaaaata ctatgtttac cttaaagatg cggcccatgc
     5581 ggacaatatt cggacaaaag aagagattaa acgtcagaag caggaacgca gtcataatca
     5641 taactcaaga gcagataatg ctgttgctgc agccagagcc cagggacgct acaccacaga
     5701 tgatggttat atctttaatg cttctgatat cattgaggac acgggtgatg cttatatcgt
     5761 tcctcacggc gaccattacc attacattcc taagaatgag ttatcagcta gcgagttagc
     5821 tgctgcagaa gcctattgga atgggaagca gggatctcgt ccttcttcaa gttctagtta
     5881 taatgcaaat ccagctcaac caagattgtc agagaaccac aatctgactg tcactccaac
     5941 ttatcatcaa aatcaagggg aaaacatttc aagcctttta cgtgaattgt atgctaaacc
     6001 cttatcagaa cgccatgtag aatctgatgg ccttgttttc gacccagcgc aaatcacaag
     6061 tcgaacagcc agaggtgtag ctgtccctca tggtaaccat taccacttta tcccttatga
     6121 acaaatgtct gaattggaag aacgaattgc tcgtattatt ccccttcgtt atcgttcaaa
     6181 ccattgggta ccagattcaa gaccagaaca accaagtcca caaccgactc cggaacctag
     6241 tccaagtc
//
LOCUS       DBERCA010000076         6125 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.076, whole genome shotgun sequence.
ACCESSION   DBERCA010000076 DBERCA010000000
VERSION     DBERCA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.076"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            279..1824
                     /locus_tag="ACL719_001828"
     rRNA            279..1824
                     /locus_tag="ACL719_001828"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1876..1948
                     /locus_tag="ACL719_001829"
     tRNA            1876..1948
                     /locus_tag="ACL719_001829"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1909..1911,aa:Ala,seq:tgc)
     gene            2073..4974
                     /locus_tag="ACL719_001830"
     rRNA            2073..4974
                     /locus_tag="ACL719_001830"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5051..5166
                     /gene="rrf"
                     /locus_tag="ACL719_001831"
     rRNA            5051..5166
                     /gene="rrf"
                     /locus_tag="ACL719_001831"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5171..5243
                     /locus_tag="ACL719_001832"
     tRNA            5171..5243
                     /locus_tag="ACL719_001832"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5204..5206,aa:Val,seq:tac)
     gene            5246..5318
                     /locus_tag="ACL719_001833"
     tRNA            5246..5318
                     /locus_tag="ACL719_001833"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5279..5281,aa:Asp,seq:gtc)
     gene            5343..5415
                     /locus_tag="ACL719_001834"
     tRNA            5343..5415
                     /locus_tag="ACL719_001834"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5376..5378,aa:Lys,seq:ttt)
     gene            5421..5502
                     /locus_tag="ACL719_001835"
     tRNA            5421..5502
                     /locus_tag="ACL719_001835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5455..5457,aa:Leu,seq:tag)
     gene            5521..5593
                     /locus_tag="ACL719_001836"
     tRNA            5521..5593
                     /locus_tag="ACL719_001836"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5554..5556,aa:Thr,seq:tgt)
     gene            5616..5687
                     /locus_tag="ACL719_001837"
     tRNA            5616..5687
                     /locus_tag="ACL719_001837"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5648..5650,aa:Gly,seq:gcc)
     gene            5695..5780
                     /locus_tag="ACL719_001838"
     tRNA            5695..5780
                     /locus_tag="ACL719_001838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5729..5731,aa:Leu,seq:taa)
     gene            5790..5863
                     /locus_tag="ACL719_001839"
     tRNA            5790..5863
                     /locus_tag="ACL719_001839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5824..5826,aa:Arg,seq:acg)
     gene            5879..5952
                     /locus_tag="ACL719_001840"
     tRNA            5879..5952
                     /locus_tag="ACL719_001840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5913..5915,aa:Pro,seq:tgg)
     gene            5958..6031
                     /locus_tag="ACL719_001841"
     tRNA            5958..6031
                     /locus_tag="ACL719_001841"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5992..5994,aa:Met,seq:cat)
     gene            6045..6118
                     /locus_tag="ACL719_001842"
     tRNA            6045..6118
                     /locus_tag="ACL719_001842"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6079..6081,aa:Ile,seq:cat)
BASE COUNT         1677 a         1264 c         1789 g         1395 t
ORIGIN      
        1 acacgggttc gaatcccgta cggactatgg tatgttgcgt caggaccact tgatgaaaaa
       61 aagtttaaaa aaacttaaaa atcttcaaaa aagtgttgac aagtgaaagt ggctgtgata
      121 tactaatata gttgtcgctt gagagaagca agtgacaaag acctttgaaa actgaacaag
      181 acgaaccaat gtgcagggcg ctacaacata agttgtagta ctgaacaatg aaaaaacaat
      241 aaatctgtca gtgacagaaa tgagtgagaa ctcaaacttt taatgagagt ttgatcctgg
      301 ctcaggacga acgctggcgg cgtgcctaat acatgcaagt agaacgctga aggagaagct
      361 tgcttctctg gatgagttgc gaacgggtga gtaacgcgta ggtaacctgc ctggtagcgg
      421 gggataacta ttggaaacga tagctaatac cgcataagag tggatgttgc atgacatttg
      481 cttaaaaggt gcacttgcat cactaccaga tggacctgcg ttgtattagc tagttggtgg
      541 ggtaacggct caccaaggcg acgatacata gccgacctga gagggtgatc ggccacactg
      601 ggactgagac acggcccaga ctcctacggg aggcagcagt agggaatctt cggcaatgga
      661 cggaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaagctctg
      721 ttgtaagaga agaacgagtg tgagagtgga aagttcacac tgtgacggta tcttaccaga
      781 aagggacggc taactacgtg ccagcagccg cggtaatacg taggtcccga gcgttgtccg
      841 gatttattgg gcgtaaagcg agcgcaggcg gttagataag tctgaagtta aaggctgtgg
      901 cttaaccata gtaggctttg gaaactgttt aacttgagtg caagagggga gagtggaatt
      961 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccggtggcga aagcggctct
     1021 ctggcttgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct
     1081 ggtagtccac gctgtaaacg atgagtgcta ggtgttagac cctttccggg gtttagtgcc
     1141 gtagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
     1201 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
     1261 accttaccag gtcttgacat ccctctgacc gctctagaga tagagttttc cttcgggaca
     1321 gaggtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc
     1381 cgcaacgagc gcaaccccta ttgttagttg ccatcattta gttgggcact ctagcgagac
     1441 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
     1501 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
     1561 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
     1621 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
     1681 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc
     1741 cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga
     1801 aggtgcggct ggatcacctc ctttctaagg ataaggaact gcgcattggt cttgtttagt
     1861 cttgagaggt cttgtggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
     1921 gtcagcggtt cgatcccgct aggctccatt ggtgagagat caccaagtaa tgcacattga
     1981 aaattgaata tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa
     2041 taagagttta tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga
     2101 tgccttggca ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg
     2161 taagtaagcg atgatccagg gatttccgaa tgggggaacc caacaggtaa tacctgttac
     2221 ccacatctgt taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc
     2281 aggaagagaa agcaaaagcg attgccttag tagcggcgag cgaaacggca gaagggcaaa
     2341 ccgaagagtt tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga
     2401 atgatttggg aagatcagcc aaagagagta atagcctcgt atttaaaata gtctttgtac
     2461 ttagcagtat cctgagtacg gcgggacacg tgaaatcccg tcggaatctg ggaggaccat
     2521 ctcccaaccc taaatactcc ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg
     2581 aaaagcaccc cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg
     2641 agcccgttaa tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat
     2701 gcgaggttaa gttgaagaga cggagccgta gggaaaccga gtctgaatag ggcgccttag
     2761 tatcatgacg tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgcggtaa
     2821 gacgcactgg aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc
     2881 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
     2941 cgtcgacatt agagattctt ggaggtagag cactgtttgg gtgaggggtc catcccggat
     3001 taccaatctc agataaactc cgaatgccaa tgaattatgg tcggcagtca gactgcgagt
     3061 gctaagatcc gtagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaataat
     3121 tgttaagtgg aaaaggatgt ggggttgcac agacaactag gatgttagct tagaagcagc
     3181 tattcattca aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac
     3241 cggggctaaa acaatttacc gaagctgtgg atacctttat aggtatggta ggagagcgtt
     3301 ctatgtgtga tgaaggtata ccgtgaggag tgctggaacg catagaagtg agaatgccgg
     3361 tatgagtagc gaaagacagg tgagaatcct gtccaccgta agactaaggt ttccagggga
     3421 aggctcgtcc gccctgggtt agtcgggacc taaggagaga ccgaaaggtg tatccgatgg
     3481 acaacaggtt gatattcctg tactagagta tgtagtgatg gagggacgca gtaggctaac
     3541 taaagcagac gattggaaga gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt
     3601 aattctataa cattgagctg tgatggggag cgaagtttag tagcgaagtt agtgacgtca
     3661 cactgccaag aaaagcttct agcgtttaaa catactctac ccgtaccgca aaccgacaca
     3721 ggtagtcgag gcgagtagcc tcaggtgagc gagagaactc tcgttaagga actcggcaaa
     3781 atgaccccgt aacttcggga gaaggggtgc tgacttaaag tcagccgcag tgaataggcc
     3841 caagcaactg tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg
     3901 tgacgcctgc ccggtgctgg aaggttaaga ggagtgctta gcgtaagcga aggtatgaat
     3961 tgaagcccca gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     4021 cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac
     4081 tcggtgaaat tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc
     4141 atggagcttt actgcagttt gatattgagt gtctgtacca catgtacagg ataggtagga
     4201 gtctaagaga tcgggacgcc agtttcgaag gagacgctgt tgggatacta cccttgtgtt
     4261 atggccactc taacccagat aggtgatccc tatcggagac agtgtctgac gggcagtttg
     4321 actggggcgg tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt
     4381 ggaaatcatt cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga
     4441 gcagggacga aagtcgggct tagtgatccg gtggttccgt atggaagggc catcgctcaa
     4501 cggataaaag ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg
     4561 gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt
     4621 tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc
     4681 ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac
     4741 cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat
     4801 gtagggaagg gataaacgct gaaagcatct aagtgtgaaa cccacctcaa gatgagattt
     4861 cccatgatta tatatcagta agagccctga gagatgatca ggtagatagg ttagaagtgg
     4921 aagtgtggcg acacatgtag cggactaata ctaatagctc gaggacttat ccaaagtaac
     4981 tgagaatatg aaagcgaacg gttttcttaa attgaataga tattcaattt tgagtaggta
     5041 ttactcagag ttaagtgacg atagcctagg agatacacct gtacccatgc cgaacacaga
     5101 agttaagccc tagaacgccg gaagtagttg ggggttgccc cctgtgagat agggaagtcg
     5161 cttagctcta gggagtttag ctcagctggg agagcatctg ccttacaagc agagggtcag
     5221 cggttcgatc ccgttaactc ccaaaggtcc cgtagtgtag cggttatcac gtcgccctgt
     5281 cacggcgaag atcgcgggtt cgattcccgt cgggaccgtt taaggtaacg caagttattt
     5341 tagactcgtt agctcagttg gtagagcaat tgacttttaa tcaatgggtc actggttcga
     5401 gcccagtacg ggtcatatat gcgggtttgg cggaattggc agacgcacca gatttaggat
     5461 ctggcgctta acggcgtggg ggttcaagtc ccttaacccg cattaagata taacaaatga
     5521 gccggcttag ctcagttggt agagcatctg atttgtaatc agagggtcgc gtgttcaagt
     5581 catgtagccg gcatttttag atagaagaaa acagtgcgaa cgtagttcag tggtagaaca
     5641 ccaccttgcc aaggtggggg tcgcgggttc gaatcccgtc gttcgcttag agaggccggg
     5701 gtggcggaac tggcagacgc acaggactta aaatcctgcg attggtaacg atcgtaccgg
     5761 ttcgattccg gtcctcggca taatatggag cacccttagc tcaactggat agagtacctg
     5821 actacgaatc aggcggttag aggttcgact cctctagggt gcatttttct atttaactcg
     5881 ggaagtagct cagcttggta gagtacttgg tttgggacca aggtgtcgca ggttcgaatc
     5941 ctgtcttccc gattcatggc ggtgtagctc agctggctag agcgtccggt tcatacccgg
     6001 gaggtcgggg gttcgatccc cttcgccgct ataatgatct tgtcggacct ttagctcagc
     6061 tggttagagc tctcggctca taaccgagtg gtcgtaggtt caagtcctac aaggtccatt
     6121 tgaat
//
LOCUS       DBERCA010000077         5927 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.077, whole genome shotgun sequence.
ACCESSION   DBERCA010000077 DBERCA010000000
VERSION     DBERCA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.077"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            54..1004
                     /locus_tag="ACL719_001843"
     CDS             54..1004
                     /locus_tag="ACL719_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699747.1"
                     /translation="MKKYILKKVLFMIPMLLIISFLVFIALDLTPADPLTYMVSPDMA
                     SSAEQIEKLRQQLGLNDPVLVRYGRWLWQLLHGDFGYSIVNGNPISKIVGQALPATFE
                     LAFVSLIISTIVGIVIGVISAVKQNGIIDNVARFLAVIGTAIPQFFFGILILNFFAIQ
                     LRILPIGGRFASGDFTFINRVEHLILPVMAMSIALVAALMRYTRNSMLDVFNADYVKT
                     ARAKGVPEWKVYFKHIFRNAMRPILVLLIFRLPMLIGGSVIIESVFSWPGIGSIILSS
                     VTAGDYPVIMITTLMISVVVLFASLLVDIMAAILDPRIRY"
     gene            1021..1872
                     /locus_tag="ACL719_001844"
     CDS             1021..1872
                     /locus_tag="ACL719_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699748.1"
                     /translation="MKPMPLWKRNLKKFYNNKLAFIGFICFMLILLACILAPLLTTYS
                     PDVVDLGSMNRPPSAKHILGTDKLGRDVFARILYGGRVSIQVGMYGAICGAVIGTVLG
                     GIAGYFGGKIDALLVRLAELFLTFPNMIVILLLSSIFGQGVFNLIFVFSVMGWMTTFR
                     MVRNEFMSLKQETYVDVCRAFGFSDSRIIFNNILRNAISPVIVSLSLNVAGFILSEAG
                     LSFLGVGVPSDIPTWGNIINAAKTADVIKNSWWLWLVPGSIITLFVLSINFIGDGLRD
                     IMDPKQQ"
     gene            1885..3867
                     /locus_tag="ACL719_001845"
     CDS             1885..3867
                     /locus_tag="ACL719_001845"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGS0699749.1"
                     /translation="MNQTVLDVKNLKTEFITDKKAFPIIKDVNFTLERGKVLGIVGES
                     GCGKSVTVNTVLNLLPKNGRIAEGEITYFDEDKPIELQKLKQYGKEFRKLRGENISMI
                     FQDPMSALNPVYTIGNQITEVLHEHYDISKEEANKRAIEMLEKLGIHNAAERMNDYPH
                     QFSGGQRQRIVIAIAMVCNPDILIADEPTTALDVTIQAQILDLLDDLRKEHGTSIILI
                     THDLGVIAQIADEVAVMYAGEVVEVGTVAQIFDQPKHPYTRSLLRSIPNPENMDKKLH
                     VIQGSVPSISEISEKGCRFANRIPWLEKEHHEEEPQLHDLGDGHFVRCSCYKHFFFED
                     KEEETLAEKHVGNVVLEVKNIKKYYYPKKQLFKPLGSPLKALDDVSLELRKGTTIGIV
                     GESGSGKSTIAKSLMKLHDITDGEINIDLNGKTQNIYGIKRKEDLDFRKKVQMVFQDP
                     YASLNPTKKIYNSFDEPMIVHNIGNKEERFERMKEALKMVNVPVEYLERYPHEFSGGQ
                     RQRLCIARALCMKPEILILDEPVSALDLSVQAQVLNYLVEIQNKEDLTYVFISHDLGV
                     VKYMCDYLYVIHKGRIVEAGSREDIYNNPMHIYTKKLLAAIPEVDYHFKEALATKRKE
                     NEVEFKAQYHEFYDEDGRAYDLKQVSPTHFVALKPE"
     gene            3877..5286
                     /locus_tag="ACL719_001846"
     CDS             3877..5286
                     /locus_tag="ACL719_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699750.1"
                     /translation="MKLNKKIYFFDKGIFNPYRMSNVKLKVNQAVKDKEIFFTEEKPW
                     EIRFDNSYPNVFFDDLAGVYRCYYSTFTDDEESEKYSLEERKERQYLPRAKRIVSLCY
                     AESKDGVNWVKPNLGITEFRGSTENNIIGHFLHGTSVFLDKHDADENRRYKMFTKIDY
                     GNGVHFIAVAFSKDGLHFDEYIKVPNFNPRADTHNHIIYDEALNRYVLITRTWRDSLR
                     LPCVSTSADFINWTPIQEILNVCDYENQIYSMPIFKRGDYILGLASVFHEGDQLNKNY
                     DTVDLQLTYSYRHVGWHYLNTDTPLIPRGKGQYGDGEFDCGCIYSSAPVTIGDRTYFY
                     YMGGNGQHTNFRETSLSRAYIEKDHFAYWDTKRPEYPGVLYTNGFIFLNDQVYLDADI
                     AAGGFVTIELFENNHTPMEITASLEKIEDGRYQVLFSEPLPRTQTRLKISFKNAKIYA
                     IEGNLDIFRIESDNALLRG"
     gene            5291..5779
                     /locus_tag="ACL719_001847"
     CDS             5291..5779
                     /locus_tag="ACL719_001847"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGS0699751.1"
                     /translation="MKYDLYDNCIELLKEREVTIEDMAALVIFSQQKYYPELTLDDAS
                     YAIQRVLKKREVQNVIMTGIELDKLAEAQKLSPEFQKIMEKDNPLYGIDEVIVLSILN
                     LYGSIAFTNYGYLDKLKPLILERLNENHEGVCNVFLDDIVGAIAAAACSKIAHNHASD
                     EI"
BASE COUNT         1944 a          884 c         1129 g         1970 t
ORIGIN      
        1 ctggtttatt gaatcatgta tctctcactc ttgttttttc aggagaaatt aaaatgaaaa
       61 aatatattct aaaaaaagta ttattcatga ttcctatgtt gttgattatt agctttttag
      121 tattcattgc actagattta acaccagcag atccattgac atatatggta tctccagata
      181 tggctagtag tgcagaacaa atcgaaaagc taagacagca attaggttta aatgatccag
      241 tattagttcg ctatggaaga tggctttggc aattactcca tggtgatttt ggatacagta
      301 ttgtaaatgg taacccaatt tccaaaattg taggacaagc attgcctgca acttttgaat
      361 tagcttttgt gtcattaatt atttcaacaa ttgttggtat tgtaattggg gttatatcag
      421 ccgttaaaca aaatggtatt atcgataatg ttgctcgttt tctagctgtt atcggaactg
      481 cgattccaca atttttcttt ggtattttaa ttttaaactt ctttgcaatt cagttaagaa
      541 ttttacctat tggtggtaga tttgccagtg gagattttac atttattaat agagttgaac
      601 acttaattct accagtaatg gctatgtcaa ttgcattggt agctgcttta atgagataca
      661 cacgtaattc tatgttggat gtttttaatg cagattatgt taaaacagct cgtgcaaaag
      721 gtgttcctga atggaaagtt tattttaaac acatatttag aaatgctatg cgaccaattt
      781 tagtgttatt gattttccgt ctaccaatgt taattggtgg atcagtaatt atcgagagtg
      841 tcttttcttg gcctggaatt ggttctatca tcctatctag tgtaacagca ggtgactatc
      901 ctgtaattat gattaccaca ttaatgattt ctgtcgtagt gttatttgct agtctacttg
      961 tagatattat ggctgcaata ttagacccac gtattcgtta ttaggaagga ggtcaatgtt
     1021 atgaaaccta tgcctttatg gaaaagaaat ttgaaaaaat tctataataa caaattggcc
     1081 tttatcggct ttatatgctt catgctaatt ctattagcat gtattctagc acctttattg
     1141 acaacctatt ctccagatgt tgttgattta ggatctatga accgtcctcc ttcagcaaaa
     1201 cacattctgg gaacagataa actaggtcgt gacgtatttg ctcgaatcct atatggtggt
     1261 cgagtttcaa ttcaagtcgg gatgtatgga gcgatttgtg gagcggttat tggtactgta
     1321 cttggtggta ttgctggata ttttggtggg aaaattgatg ctcttctagt tcgtctagca
     1381 gaattattct taacattccc gaatatgatt gtcattctct tgctttcaag tatttttgga
     1441 caaggtgtat tcaatcttat cttcgtattc tcagtaatgg gttggatgac aacgttccga
     1501 atggttcgta atgagttcat gtctttgaaa caagaaacat atgtggatgt atgtagagct
     1561 tttggttttt ctgacagtcg aattatcttt aataatattt taagaaatgc tatctcacca
     1621 gtaattgtat cgttgtcttt aaacgttgct ggtttcattc tcagtgaggc aggtcttagt
     1681 ttcttaggag taggtgttcc ttctgatatt cctacttggg gaaatatcat aaatgctgcc
     1741 aaaacagccg atgttattaa aaatagttgg tggttgtggt tagttccagg gtcaatcatt
     1801 actcttttcg ttctttctat caactttatt ggtgatggat tacgagatat aatggatccg
     1861 aaacaacagt aggaggttga caaaatgaat caaacagttt tagatgtaaa aaacttaaaa
     1921 actgaattca tcactgataa aaaagcattt ccgattatta aagatgtaaa cttcactttg
     1981 gaaagaggga aagttttagg gattgttggt gaatctggat gcggtaagag tgtaacagtg
     2041 aatacagtgt taaacttatt acctaaaaat ggacgcattg ctgaaggtga aatcacatat
     2101 ttcgatgaag ataagccgat agagcttcaa aaattaaagc aatatggtaa agaatttcga
     2161 aagttacgtg gtgaaaacat atcaatgatt ttccaagatc caatgtctgc attgaatcca
     2221 gtatatacaa ttggtaatca aattactgaa gtccttcatg aacattatga catttctaaa
     2281 gaggaggcaa ataaacgtgc tatagaaatg ttagaaaaac ttggaattca taatgctgca
     2341 gaacgcatga atgactatcc acaccaattt agtggagggc agcgtcaaag aatagtcata
     2401 gcgattgcaa tggtatgtaa tccagatatt ctaattgcag atgaaccaac aactgccctt
     2461 gatgtaacaa tccaagcaca aatacttgat ttacttgatg atttacgtaa agagcatgga
     2521 acttctatta tcttaattac tcacgattta ggcgttattg ctcaaattgc ggatgaagtt
     2581 gcagtgatgt atgctggtga agtcgttgaa gttggaactg tagctcagat ctttgatcaa
     2641 cctaaacacc cgtatactcg ttctctatta agatctattc ctaatcctga aaatatggat
     2701 aaaaagctac atgttatcca ggggagcgta ccttcaattt ctgaaatatc tgaaaaagga
     2761 tgcagattcg caaatcgtat tccatggtta gaaaaagaac atcatgaaga agaacctcaa
     2821 ttgcatgatt taggtgacgg acattttgta aggtgttctt gctataaaca tttctttttc
     2881 gaagataagg aagaagaaac attagcggaa aaacatgtag gtaatgttgt tttagaagta
     2941 aaaaatatta aaaagtacta ctatcctaag aagcaactat tcaagccact aggttctccg
     3001 ttgaaagcac ttgatgacgt atctctagag ttaagaaaag gaactactat tggtatcgta
     3061 ggagagtctg gaagcggtaa gagtacgatt gcgaaatcat taatgaaact ccatgatatt
     3121 acagatggtg aaatcaatat tgatttgaat gggaaaactc aaaatattta tggaattaaa
     3181 cgtaaggaag atttggattt ccgtaagaaa gttcaaatgg tattccaaga tccttatgca
     3241 tcattgaacc caactaagaa aatttataat tcatttgatg aaccgatgat tgtccataat
     3301 attggaaaca aagaggaacg ctttgaacgt atgaaagaag ccttgaagat ggtaaacgtg
     3361 ccagttgagt acttggagcg ttatcctcat gaattttcag gcggacaacg tcaacgttta
     3421 tgtattgctc gtgccctttg tatgaaacca gaaattttaa ttttagatga acctgtttca
     3481 gcattagatc tatctgtaca agcgcaagtt ttgaattatc ttgttgaaat ccaaaataaa
     3541 gaagatttga cttatgtatt tatttctcat gacctaggtg ttgtgaaata catgtgtgac
     3601 tatctatatg ttattcataa aggacgtatt gtagaagctg gttctcgaga agatatctac
     3661 aataacccaa tgcatattta cactaaaaaa ctattagctg ctattccaga agttgattat
     3721 cacttcaaag aggcattggc aacaaaacgt aaagaaaatg aagttgaatt taaagcacag
     3781 taccatgaat tttatgatga agatggtcgt gcttatgatc ttaagcaggt ttcaccaaca
     3841 cattttgtag cgttaaaacc agaataggtg gtaaatatga aattaaataa aaaaatatat
     3901 ttctttgata aaggaatttt taatccttat cgaatgagta atgtaaaatt aaaagttaat
     3961 caggcagtaa aggataaaga aattttcttc actgaggaaa aaccatggga aattcgtttt
     4021 gataacagtt atccaaatgt tttctttgat gatttagcag gtgtttatcg ttgctactat
     4081 tctacattta ctgatgatga ggagtctgaa aaatactctt tagaggagcg taaggaacgt
     4141 caatatctac ctcgtgcgaa gcgtattgtt tcactttgtt atgctgaaag taaagatggt
     4201 gttaactggg tgaaaccaaa tctaggtatt acagaatttc gaggatcaac tgaaaataat
     4261 attatcggac attttttaca tggtacatct gtttttctag ataaacatga cgcagatgaa
     4321 aatcgtcgtt ataaaatgtt tacaaagatt gattacggta acggtgttca tttcattgct
     4381 gtagcatttt caaaagatgg tctacacttt gatgaataca ttaaagtacc aaacttcaat
     4441 ccacgtgcag acactcataa ccatatcata tatgatgaag cattaaatcg ctatgtgcta
     4501 attactcgta cttggagaga ttcattgcgc ttgccgtgtg tatctacgag tgctgatttc
     4561 attaactgga ctccaattca agaaattttg aatgtctgtg attatgaaaa tcaaatttat
     4621 tctatgccta tttttaaacg tggcgattat atactaggtt tagcttcagt tttccatgaa
     4681 ggagatcaac tgaacaagaa ttacgataca gtggaccttc aattgactta tagttatcgt
     4741 catgttggtt ggcattatct aaacactgat actccactta ttcctcgtgg taaaggtcaa
     4801 tatggtgatg gagaatttga ctgtggatgt atctattctt ctgcaccagt tactatcggt
     4861 gaccgtactt atttctacta catgggagga aatggacaac atacaaactt ccgtgaaaca
     4921 agcttgtctc gtgcttatat tgagaaagat cactttgcat attgggatac taaacgtcca
     4981 gaatatccag gtgttttgta taccaatgga tttattttcc tcaatgatca agtttacctt
     5041 gatgcagata ttgcagctgg aggctttgta acaattgaat tgttcgagaa taatcacact
     5101 ccaatggaaa ttacagcttc ccttgaaaaa atagaagatg gtcgctatca agtgttattc
     5161 agcgaacctt tacctcgaac tcaaactcga ctgaaaatta gctttaaaaa tgctaaaatc
     5221 tacgcaatcg aaggaaattt agatattttc agaattgaat cagacaatgc tctattgagg
     5281 gggtaagatt atgaaatatg atttatatga taattgtatt gaacttctaa aagaacgcga
     5341 agttacaatt gaagatatgg cagcgttagt aatattttca caacaaaaat attaccctga
     5401 attaactctt gacgacgctt catatgcaat tcaacgtgta ctaaaaaaac gtgaagttca
     5461 gaatgtaatt atgacaggaa ttgagttaga taagttagct gaagcacaaa aactatctcc
     5521 agaatttcaa aagattatgg agaaagataa tccattgtat gggattgatg aagtaatcgt
     5581 cttatcgata ttgaatttat atggttctat tgcctttaca aattatggct atttagataa
     5641 attaaaacca ctgattttag aaagattgaa tgagaatcac gaaggtgttt gtaatgtatt
     5701 tctagatgat attgttggtg caatagccgc agcagcatgt agtaaaattg cacacaatca
     5761 tgctagtgat gaaatataac tattgaattt agtatcgtaa ataataaagc gtttggtttc
     5821 tagattattg tcattcaaat tggataatga taaatctaga aaccaaatat attttccgca
     5881 taaaatgagg gattatatat ctcacttttt tacactagaa tagtgca
//
LOCUS       DBERCA010000078         5652 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.078, whole genome shotgun sequence.
ACCESSION   DBERCA010000078 DBERCA010000000
VERSION     DBERCA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.078"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(125..841)
                     /locus_tag="ACL719_001848"
     CDS             complement(125..841)
                     /locus_tag="ACL719_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0699752.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1189..2976
                     /locus_tag="ACL719_001849"
     CDS             1189..2976
                     /locus_tag="ACL719_001849"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGS0699753.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2973..3449
                     /locus_tag="ACL719_001850"
     CDS             2973..3449
                     /locus_tag="ACL719_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699754.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3477..4382
                     /locus_tag="ACL719_001851"
     CDS             3477..4382
                     /locus_tag="ACL719_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS0699755.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVVGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4369..5184
                     /locus_tag="ACL719_001852"
     CDS             4369..5184
                     /locus_tag="ACL719_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0699756.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5191..5595
                     /locus_tag="ACL719_001853"
     CDS             5191..5595
                     /locus_tag="ACL719_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0699757.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         1580 a         1046 c         1244 g         1782 t
ORIGIN      
        1 ttggttctat ttctataaaa tattccacaa attatagaat tttccaaaaa taggtaagcg
       61 ctaccttttt ggtgtggtat aataagcata gaaaaagccc aagcgattag ctcaggcttt
      121 cttcttagtg atcacggtca catgagataa atttaatctt gtagtaatca gatcgtttgt
      181 aagtttcact gtattctaaa acttggccag ttgattcgag tttggtgatt ttagtttgta
      241 ggacagtagg gaattgttca tcgactccga ggactgaagc cgcatgttct ggagttggaa
      301 agactatttc gttgatttct tcaaagtgtt catcattcat gtgaatgtgg tagtctaact
      361 tgaaacgatt atagatagaa ctatagtatt caaggtttgg ataatttgcg ttgatatatt
      421 gttctgggat gtaggatgta tggtagatat aaacgacacc gtttgattcg cggatacgtt
      481 caatcttgta gtagaattga tcgccacgta gacccaattt ttccaagtaa acaagcttgt
      541 ttccgcgttc aattgaaaga acagttacct tatcatcttt agcattgaag agttcaatat
      601 ctgaaaactc tacaagcttg tgtttgcgtg cacgtgaaac gaaggttcct tttccttgtt
      661 ggcggacaat atagccatct ttggcaaggt cgtttaaggc gcgaacaact gtgatagagc
      721 tgacatcgta cattgaaatg agttctgctt cagtgtaaaa tttatctcca ctgctaaact
      781 gcccagagat gattttattt tttaattcgt cttttatgta ttgatatttg ggaattgcca
      841 tatttttacc tctattttcc ttctccatag aaaattttac cataaaagcg aaaaaaatag
      901 tagtcttttg aataaaaaat tagaataata gtctgaaaaa ttaagcgata tagggattgc
      961 tcatatttta tcaatgtatt ctatacaaaa ttattttagg tcctatattc cgaatttaaa
     1021 gagcgtttga aatgggtcta aaaatgtttg aaactaattg aatgtgtgta taatagtaaa
     1081 aatgcagtaa atatcccgac aatttctatt gacaattcaa acagattggt ttataattaa
     1141 tataacaaca aatgaaagcg caaactttcg cggtcggaag gtagttttat gacacgattt
     1201 gagatacgag atgatttcta tctcgatgga aaatcattta agattttatc tggtgccatt
     1261 cattatttta gggttcctcc agaggattgg tatcattcgc tctataactt gaaggctctt
     1321 ggttttaata cggtagagac ttatgttgct tggaatttac acgagcctcg tgaaggtgag
     1381 tttcattttg aaggtgatct ggatttagag aaatttctcc aaatagcgca ggatttgggt
     1441 ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg aatgggaatt cggtggctta
     1501 ccagcttggc tcttgaccaa gaacatgcga attcgctcat ccgacccagc atatatcgag
     1561 gcagttggtc gctactatga tcagttattg ccaagactgg tgcctcgttt gttggacaat
     1621 ggtggcaata ttctcatgat gcaggttgaa aatgagtatg gttcttacgg agaagataag
     1681 gcttacctga gagcgattcg acagctaatg gaagagtgtg gcgtaacctg tcccctcttt
     1741 acatcagatg gtccatggcg agctactctg aaagctggaa ccttaattga agaggacctc
     1801 tttgtaacag gaaactttgg ttctaaggca ccttacaact tttcgcagat gcaggaattc
     1861 tttgatgaac atggtaagaa atggccactc atgtgtatgg agttctggga tggttggttc
     1921 aatcgctgga aagaaccgat tatcacacgg gatcctaagg aattggcaga tgcagttcga
     1981 gaggttttgg aacaaggctc tatcaatctt tacatgttcc acggtggtac aaactttggt
     2041 ttcatgaatg gttgctcagc tcgaggaact ttggacctgc cacaagttac gtcttatgat
     2101 tacgatgccc ttctggatga agaaggaaat ccaactgcta aatatcttgc agtcaagaag
     2161 atgatggcaa cacatttttc agagtatccg cagttggaac cactctacaa agagagtatg
     2221 gagttggatg ctattccact agttgaaaaa gtttctttgt ttgaaacctt agatagcttg
     2281 tcaagtcctg tagaaagtct ctatcctcaa aagatggagg agctgggaca aagttatggc
     2341 tacctacttt atcgaacaga aacaaactgg gatgcagaag aagaaagact tcgtatcatt
     2401 gatggtcgag atagggccca gctgtatgtc gatggtcagt gggttaaaac tcaatatcag
     2461 acagagattg gggaagatat tttttatcaa ggtaaaaaga aagggctatc taggttagat
     2521 atcttgatag aaaatatggg gcgtgtcaac tatgggcata agttcttagc ggatacgcaa
     2581 cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt tcttactaaa ctggaaacac
     2641 tatccactcc cactagacaa tcctgagaaa attgattttt caaaaggatg gactcaagga
     2701 caaccagcct tttacgctta tgactttaca gtcgaagagc caaaagatac ttacctagac
     2761 ttgtctgagt ttggtaaggg ggttgccttt gtcaatgggc agaatctagg acgtttttgg
     2821 aacgttggcc caactctctc actttatatc cctcatagct atctcaagga aggtgccaac
     2881 cgcatcatta tctttgaaac agaaggtcaa tataaagaag agattcattt aactcgtaaa
     2941 cctacactaa aacatataaa gggggaaaac ttatgacaat tgtaggatgc cgtattgatg
     3001 gacgtttgat ccacggacaa gtagccaatc tttgggctgg aaaactaaat gtttcacgca
     3061 ttatggttgt agacgacgaa gttgtcaaca acgatattga aaagagtggt ttgaaacttg
     3121 cgacaccacc aggtgtgaaa ttgagtattt tgccagttga gaaagctgca gccaatattc
     3181 ttgctggcaa atacgatagc caacgtctct ttatcgtggc tcgtaaacca gaccgcttcc
     3241 ttggtttggt agaagcaggt gtaccacttg aaacccttaa tgttgggaat atgtctcaaa
     3301 caccagaaac tcgttctatt acacgttcta tcaacgtagt agacaaggat gtggaagact
     3361 tccacaaact ggcagaaaaa ggtgttaaac ttactgctca gatggttcca aatgatccaa
     3421 tttcagactt tttgagctta ttaaaatagg aaaaaaattt ttaggaggtc attgttatga
     3481 tacaatggtg gcaaatttta cttctcactt tgtactcagc ttatcaaatc tgtgatgagt
     3541 tgacgatcgt ttcatctgca ggttcccctg tatttgctgg tttcattact ggtttaatca
     3601 tgggagatgt gactactggt ttacttatcg gtggtaactt gcaactgttc gttcttgggg
     3661 ttggtacctt cggtggtgct tctcgtatcg acgcaacttc tggtgcggtt cttgcgacag
     3721 ccttctctgt ttcacaagga attgatgcac cgcttgccat tactacaatc gctgtaccag
     3781 tagcagctct cttgacttac ttcgacgttc ttggtcgtat gactactacc ttcttcgctc
     3841 accgtgtgga tgctgcaatc gaacgctttg actataaagg tattgaacgc aactacttgc
     3901 ttggtgcgat tccgtgggct ctatctcgtg cccttccagt cttctttgcc cttgcttttg
     3961 gtggtgcctt tgtacaatca gtagtagact tcgttgaagc ctacaaatgg gttgcagatg
     4021 gcttgacact tgcaggacgt atgcttccag gtcttggatt tgcaatcttg cttcgttacc
     4081 ttccagttaa acgtaacctt cactaccttg ctatgggatt tggtttgaca gctatgttga
     4141 ctgttcttta ctcatatgta acaggtcttg gtggcgctgt tgttggtatc gtaggtactc
     4201 ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa cttcaaaggt ttgtctatga
     4261 ttggtatttc tatcgtaggt attttccttg cagtgcttca cttcaaaaat agccaaaaag
     4321 tagctgtagc agcaccttct acaccatcag aaagtgggga aatcgaagat gacgaattct
     4381 aattacaaac ttacaaaaga agattttaat caaatcaaca aacgtagctt gtttactttc
     4441 caattaggtt ggaactacga acgtatgcaa gcttctggtt acctttacat gatcttgcct
     4501 cagttgcgta aaatgtatgg tgatggaact cctgaattga aagaaatgat gaaagttcat
     4561 actcaattct tcaatacttc accattcttc cataccatta tcgctggttt tgaccttgcc
     4621 atggaagaaa aagatggtgt aggttcaaaa gacgccgtta acggtatcaa gacaggtttg
     4681 atgggaccat tcgctcctct tggggataca atctttggtt cacttgtacc tgctatcatg
     4741 gggtcagtcg cagcaactat ggctatcgct ggccaacctt gggggatctt cctttggatt
     4801 gcagttgcag tagcgtatga catcttccgt tggaaacagt tggaatttgc ttacaaagaa
     4861 ggggttaacc ttatcaacaa catgcaaagt accttgacag ctttgattga cgctgcatct
     4921 gtacttggtg tcttcatgat gggtgctctt gtagcaacag tgattaactt tgaaatttct
     4981 tacaagttgc caatcggtga aaagatgatt gatttccaag acatcttgaa ccaaatcttc
     5041 ccacgtttgc ttccagcaat ctttactgcc tttatcttct ggttgcttgg taagaaaggt
     5101 atgaactcta ctaaagctat cggtattatt atcgtacttg ctttggctct ttctgccctt
     5161 ggtcactttg cacttggaat gtaattcctt atgactaaat cattaatttt ggtgagccat
     5221 ggtcgcttct gtgaggagct tagaggtagc acagaaatga ttatgggccc acaagacaac
     5281 atttacacag tagctcttct tccagaagat ggcccagaag aatttactgc taaatttgaa
     5341 gctgttattg aaggattgga tgatttccta gtctttgcgg atcttctcgg tgggacacct
     5401 tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata ttgaccttta cgcagggatg
     5461 aatcttccaa tggtgattga atttatcaat gcgagcctta caggcgcaga tgcggactac
     5521 aagagccgtg ctgcagaaag cattgtgaaa gttaatgacc tgttagcggg cttcgatgat
     5581 gacgaagatg aataatactc ttcgaaaatc tcttcaaact acgtcaacgt cgccttgccg
     5641 taggtatatg tt
//
LOCUS       DBERCA010000079         5314 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.079, whole genome shotgun sequence.
ACCESSION   DBERCA010000079 DBERCA010000000
VERSION     DBERCA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.079"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            84..881
                     /locus_tag="ACL719_001854"
     CDS             84..881
                     /locus_tag="ACL719_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGS0699758.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSLLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1029..2288
                     /locus_tag="ACL719_001855"
                     /pseudo
     CDS             1029..2288
                     /locus_tag="ACL719_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            2384..3658
                     /locus_tag="ACL719_001856"
     CDS             2384..3658
                     /locus_tag="ACL719_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGS0699759.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3670..>5314
                     /locus_tag="ACL719_001857"
     CDS             3670..>5314
                     /locus_tag="ACL719_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGS0699760.1"
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYI
                     EHLIKYWQGQVLSVSGEKRLGREHHVVFDLISKSSISNKRKVDLVNYELKKVFGEDFE
                     KRRLDFEKGYFKNLLPYSIVNQTVESLASLNAIFITSNYDYEIENHIKRLKNAVVTIN
                     DLNEFTKNKNGKLQFGDVLHIHGTPDCDVKYFVSSSADYSKTYLKNRENFENLVTWFK
                     ETKPTVLFVGAGLEEDEILSLLCKDSKNYALMKSENTGNQRVDEHYRGVVEGFFSSEN
                     HTQIIWYGDEFEKLPLFVKKLVADINEKLGTHDFYNQWNNLLNPSINQEEYNKNLDSI
                     SNDFKYLSSVLDKVIENDNNQLDQLMLNALLRSETLTVIKKNFVLAFWRFIVKNIEKL
                     SDNEWDVIYKIIYEGSQNYFIDDVFFVYNYAIDNKISSFTNNNKLNELREIISKDGYI
                     VNSNFNKDKTLLGYWLVSAFEQQNRDLYIKEDSEVEVNLNYECVNKLMSILNNPEFLS
                     YNYYSIEHQLKEYDVVKFLYELVKSKKLFIEEEKFLESDS"
BASE COUNT         1745 a          847 c         1088 g         1634 t
ORIGIN      
        1 acttttatga aatatagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaaagat ttaggagttt attatggttt cttcagaatt tatctcaaag attgaatttg
      121 cttgcaataa gaaagaaagt ctttatagtc aaagcaaatt taagtatgcg attcgttcga
      181 tgttcgcagg tgcattttta accttcagta ctgctgcagg tgcagttggg gctgacttga
      241 ttaataaaat tgcaccaggt agcggacgct tcctctttcc attcgttttt gcttggggct
      301 tggcctacat tgtttttttg aatgccgagt tggtcacttc aaacatgatg ttcttgactg
      361 ctggtagttt attaaaaaaa atctcttgga gaaaaacagc tgagatttta ctatactgta
      421 ccttgttcaa ccttatcgga gccttgatag cagggtgggg ctttgctcat tcggcagcct
      481 atgcgaatct gacacacgat agtttcatct caggggttgt agagatgaag ttaggccgct
      541 caaatgaatt ggtcttgctt gaggcgattt tggcaaatat tttcgtaaac attgccattc
      601 tttcatttat tttggtcaaa gatggtggtg ccaaactttg gcttgtgttg tcagctattt
      661 acatgtttgt attcttaaca aacgagcaca ttgcggcgaa ctttgcttct ttcgcgattg
      721 tgaaattcag tgttgctgcg gattcaattg ccaacttcgg tgttggaaat atgcttcgcc
      781 actggggtgt gactttcatc ggaaacttta tcggaggagg cctcttgatg ggtcttccat
      841 atgccttcct caataaaaac gaagatactt atgtagatta agaaaatgag cacgattgag
      901 tcgtgctttt ttcattttca aaataagata atagctattt cttatatcaa aatatagaaa
      961 actgatattt gtaaactata actcaaggtg ctacaatatc cttaataaaa taatatggag
     1021 gtcaccttat gactcgtgat tttaaatttg aaactctaca actacatgct ggtcaagttg
     1081 tgactccagc tactaagtct cgtgcagtgc cgatttatca aacaacatct tttgtttttg
     1141 atgacacgta ggaaggtgct gatctgtttg ccttgaggaa accagggaac atttatactc
     1201 gtatcaccaa tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat
     1261 caggtatgac tgcagtgact tatacgattt tggcgcttgc ccatgctggt gagcatgtag
     1321 tggctgcttc gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt
     1381 atggtatcac aacaaccttt gtcgatgttg ataatttgga ggaagtagaa gcagctatca
     1441 aagacaatac caagcttgtc ttgattgaaa ccttggttaa ccccttgatt aatattccag
     1501 acctggaaaa actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata
     1561 cttttgcaac accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact
     1621 ctgcgactaa gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg
     1681 gtcgttttga ctggacggct tcagggaaat tccctcaatt tgttgacgag agtccaagct
     1741 gccacaattt gagctatact catgatgtgg gtgcagcagc ctttattata gctgttcgag
     1801 ttcaattgct tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac
     1861 aaagacttga aaccctttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg
     1921 ttgattttct tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata
     1981 gtccttatta tgccttggct gagaaatact tgccaaaagg tgtcggttca atctttacct
     2041 tccacgtcaa aggtgacgag gaggaagcac gcaaggtcat tgataattta gaaatctttt
     2101 ctgaccttgc aaacgcggca gatgctaaat cgcttgttgt ccatccagca acaatcactc
     2161 acggtcaatt gtcagaaaaa gacctagaag cagcaggtgt cacaccaaat caaatccact
     2221 tgtctatcgg tcttgaaaat gtagaagatt tgattgaaga cttacgcttg gccttggaaa
     2281 aaatttaaag taaaagaaga taaacagtgg gttttgactc actgtttttg attttccctc
     2341 aggcatgata taatggttac agaagtctag aaagaggaat gtaatgaacg aaatcaaatg
     2401 tcccaactgt ggggaagtct ttacagtaaa tgaaagccag tatgctgaac ttttgtccca
     2461 agtgagaacg gcagagtttg ataaggaact acacgatagg atgaggcagg aactggcctt
     2521 ggctgagcaa aaggctatga atgagcaaca gactaaactg gctcagaagg atcaagaaat
     2581 tgcgcaattg cagagtcaaa tccaaaactt tgatacagaa aaagaattgg ccaagaaaga
     2641 agttgaacag acaagccatc aggccttatt ggctaaggac aaggaagtac aggccttgga
     2701 aaaccagttg gcgaccttgc gtttggagca tgaaaatcaa ttgcaaaaga ccctttctga
     2761 cctagaaaaa gaacgcaatc aggtcaaaaa tcagctccta ctgcaagaaa aggaaaatga
     2821 gttgtctttg gcttctgtta agcaaaacta cgaagcccag ctcaaagcag ccagtgaaca
     2881 agtcgagttt tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct
     2941 agaacagtat gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta
     3001 ctttgagaag gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga
     3061 gtgtgatgaa aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga
     3121 cggaacagag aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtcg
     3181 ggagaagaac tgtgagtatg ccgttttggt gaccatgctt gaggctgata atgactactt
     3241 taacacaggg attgttgacg tcagtcacga gtatgaaaaa atgtatgttg ttcgtcctca
     3301 attctttatc caattgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa
     3361 gcaggagttg gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagactt
     3421 ggatgccttt aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa
     3481 agctattgat gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac
     3541 tacatctgaa aaccaactcc gtctagctaa caacaaattg gaagatgtct ctgttaaaaa
     3601 attgacccgg aaaaatccaa caatgaaagc gaagttcgaa gcattgaagg gggagtgagg
     3661 aaataagtta tggaagatga tcttaattat gaaaatttga tggatgatgt aaccgaggct
     3721 ataaaaaagt ttaacttagt aatttttatt ggcgcaggag tttcaattgc tcaaggttat
     3781 cctaattgga acaattatat tgaacatctt attaagtatt ggcaaggaca agttttatcg
     3841 gtaagtggtg aaaaaaggct tggaagagaa catcacgtag tttttgattt aattagcaaa
     3901 tctagtatta gtaataaacg taaagtggat cttgtaaact atgaattaaa aaaagttttt
     3961 ggtgaagatt ttgaaaagcg acgactagat tttgaaaaag gatattttaa aaatctatta
     4021 ccatattcaa tagttaatca aacagtagaa tctttagcaa gtttgaatgc aatatttatt
     4081 acttctaact acgactatga aattgaaaat catattaaac gtttaaagaa tgctgtagtt
     4141 actattaatg atctcaatga attcactaag aacaagaatg ggaaactaca atttggtgat
     4201 gttctacata tccatggaac accagattgt gatgtaaagt attttgtaag ttcatccgca
     4261 gattattcaa aaacctattt aaaaaatagg gaaaattttg aaaatttagt aacatggttt
     4321 aaagagacaa aacctactgt tttatttgtt ggagcaggtc ttgaagaaga tgaaatttta
     4381 tctttacttt gtaaagatag taagaactat gccttgatga agtcagagaa cacaggtaat
     4441 cagagagttg atgaacatta tagaggtgtt gttgaaggat tttttagctc agaaaatcat
     4501 actcagatta tttggtacgg agatgaattt gagaaactac ctctttttgt aaaaaaatta
     4561 gttgctgata ttaatgaaaa attgggcaca catgattttt ataatcagtg gaacaatctt
     4621 ttgaatcctt ctataaatca agaagagtat aataaaaatc tagatagtat ttctaatgat
     4681 tttaagtatc taagctctgt tttagataag gttattgaaa atgataataa tcaactagat
     4741 cagctaatgt taaatgctct gcttcggagt gaaactctaa cagtaattaa aaaaaatttt
     4801 gtccttgcat tttggaggtt tattgttaag aatattgaaa agttaagtga taatgagtgg
     4861 gatgtgattt ataaaataat ttatgaaggc tctcaaaatt actttataga tgatgtattc
     4921 tttgtctata attatgcgat agataataaa atttctagtt ttacaaataa taataagctt
     4981 aatgaactga gagaaattat atccaaagat ggatatatcg ttaattctaa ctttaataaa
     5041 gataaaactc ttttgggtta ctggctagta agtgcttttg aacagcaaaa tcgtgattta
     5101 tatataaagg aagatagtga agtagaagtt aacttaaatt acgaatgtgt taacaaatta
     5161 atgtccatac taaataatcc agaattttta agttataatt actactctat agagcatcaa
     5221 ttaaaagagt atgatgttgt taaatttctt tatgagcttg taaagtccaa aaaattattt
     5281 attgaagagg aaaagttttt agaaagtgat tctg
//
LOCUS       DBERCA010000080         5282 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.080, whole genome shotgun sequence.
ACCESSION   DBERCA010000080 DBERCA010000000
VERSION     DBERCA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.080"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            83..1072
                     /locus_tag="ACL719_001858"
     CDS             83..1072
                     /locus_tag="ACL719_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699761.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1100..1903
                     /locus_tag="ACL719_001859"
     CDS             1100..1903
                     /locus_tag="ACL719_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGS0699762.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1927..2838
                     /locus_tag="ACL719_001860"
     CDS             1927..2838
                     /locus_tag="ACL719_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0699763.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2894..4228)
                     /gene="pepC"
                     /locus_tag="ACL719_001861"
     CDS             complement(2894..4228)
                     /gene="pepC"
                     /locus_tag="ACL719_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGS0699764.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4445..5167)
                     /locus_tag="ACL719_001862"
     CDS             complement(4445..5167)
                     /locus_tag="ACL719_001862"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0699765.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         1429 a         1180 c         1078 g         1595 t
ORIGIN      
        1 tttttatgaa aacggtttat acttaaacag tcttaaagtt ttcttaaatg aaaactaaaa
       61 caaaaaagga ggaatgacaa taatgagtat cggaatcatt attgcgagcc acggcgaatt
      121 tgctgcgggt attcatcagt caggatctat gatctttggt gaacaagaaa aggttcaagt
      181 tgtgaccttt atgccaaatg aaggtcctga tgatctatac gctaagttta ataacgctgt
      241 tgctgcattt gacgcagaag atgaggttct agttttggct gacctttgga gtggttctcc
      301 atttaaccaa gctagtcgcg tgatgggaga aaatcctgag cgtaagtttg ccatcatcac
      361 aggacttaac ttaccgatgt tgattcaagc ctacacagag cgcctcatgg acgctgctgc
      421 aggtgtagaa aaagtcgctg ctaatatcat taaagaagcc aaagatggca tcaaagctct
      481 tccagaagag ctaaatccag ttgaagaagt tgcaagcgct gcagctgctc cagttgccca
      541 aactgctatc ccagaaggaa ctgttatcgg agacggtaaa ttgaaaatca atcttgcccg
      601 tcttgacaca cgtctacttc acggtcaggt tgcaactgct tggactccag attcaaaagc
      661 aaatcgtatc atcgttgctt cagataacgt ggctaaagac gaccttcgta aagaattgat
      721 taaacaagca gctccaggta atgtcaaggc taacgtggtt ccaattcaaa aactgattga
      781 gatttcaaaa gacccacgtt ttggagaaac acatgccctt atcttgtttg aaacacctca
      841 agatgccctt cgtgccatcg aaggcggcgt gccaatcaag actcttaacg ttggttctat
      901 ggctcactca acaggtaaaa cattggtcaa taccgttttg tctatggaca aagaagatgt
      961 tgctacattt gaaaaaatgc gtgacttggg tgttgaattt gatgtccgta aagtaccaaa
     1021 tgattctaaa aaagatttgt ttgacttgat taacaaagcc aatgtcaaat aagccattat
     1081 ttatgaaagg attttaaaca tgtctattat ttctatggtt ttagtagtcg ttgtagcctt
     1141 ctttgcaggt cttgaaggca tcctcgacca gttccaattt caccaaccac ttgtagcctg
     1201 tacccttatt gggcttgtaa caggtcactt ggaagcaggg attatcctcg gtggatcgct
     1261 tcaaatgatt gcccttggtt ggtcaaatat cggtgctgct atcgctcctg atgctgcact
     1321 tgcttctgtc gctgctgcca ttatcatggt tcttggtggt gactttacca agactggtat
     1381 cggtgttgcc caagcggttg ctatccctct tgccgtagct ggtctattct taactatgat
     1441 tgttcgtaca atttcagttg gattggttca cactgctgat gctgccgcta aaaaaggtga
     1501 cttcggcgct gtggagcgtg ctcatttcat tgcgctactt ttccaaggac ttcgtatcgc
     1561 gcttcctgca gctcttctcc ttatggtacc aactgaaact gtacaaagta tccttagtgc
     1621 catgccagac tggctcaaag atggtatggc tatcggtggt ggtatggtcg ttgccgttgg
     1681 ttacgccatg gttatcaaca tgatggcaac tcgtgaagta tggccattct tcgctcttgg
     1741 tttcgttctt gctgctgtgt cagatattac tctaatcgga ttcggtgcta tcggtgttgc
     1801 tatcgctctt atctaccttc acctttctaa aactggtgga aatggtggcg gaggagccgc
     1861 aacttctaac gacccaatcg gcgatatcct agaagactac taagataaga aaggactgaa
     1921 aacatcatga ctgaaaaact tcaattaact aaatcagatc gtaaaaaagt ttggtggcgt
     1981 tcaaccttct tacaagggtc ttggaacttt gaacggatgc aaaacttggg ctgggcttat
     2041 acactcattc cagctatcaa aaaactctat actaaaaaag aagatcaaat cgctgctctt
     2101 gagcgtcacc ttgagttctt caacactcat ccatacgtag ctgctccagt catgggggtt
     2161 actcttgcgc ttgaagaaga acgtgctaac ggtgtggaaa tcgatgacgc tgctatccaa
     2221 ggggttaaaa tcggtatgat gggacctctt gctggtatcg gtgacccagt attctggttt
     2281 acagtacgcc caatccttgg atctctcggt gcttcacttg cccttactgg caatatcttg
     2341 gggccactcc tcttctttgt tgcatggaac ttgattcgta tgtcattctt gtggtatgtt
     2401 caagagattg gatacaaggc tggatcagaa atcactaaag atatgtctgg tggtatcctt
     2461 caagatatca ctaaaggagc ttctatcctt gggatgttca ttcttgctgt ccttgttcaa
     2521 cgctgggtaa atattaaatt tgctttcgat gtttctaaag ttcaactaga tgaaaaggct
     2581 tatatccatt gggataaatt gccagaaggg tctaaaggta tccaagaagc attcgcacaa
     2641 gtaggacaag gattgtctca aactcctgaa aaagttacta ctttccaaca aaacttggat
     2701 atgttgattc ctggattatc aggactactc cttactttac tttgcatgta cttacttaag
     2761 aaaaaagtat ctccaatcac tattatcctt gccctcttcg cagtgggtat tgtggcacat
     2821 gttcttcaca tcatgtaatc aagcaactaa aaaggaacca ggttctaaaa tctgattcct
     2881 tttttctatg cttttattca gccaaggctc ccattggatc ccatggtgca agtacgattg
     2941 gttctgctcc ataggcagct tgttcttctg ctgtcagcaa ttccttacga acaacgattt
     3001 ggtatgtgta ttcgtccatc caagcgtctg aggcaacaaa gtaaccatct gtaccgacct
     3061 tgtctcccca tgagttttca accttccact tggttgattt accattttcg tccaagtcaa
     3121 cacctgtcaa gaccatggcg tgggtcatca agctttcgct atagtccaaa cgtccagcct
     3181 tgtcttgagt aagtttaatg tccatgcttg attcaaagtc ataaacatct gtcgcaagga
     3241 tgccagcctt acggttgctg agctggccga catcagaacc aaaccaaaca gtctcacctg
     3301 cttgcatttg ggcaatcgcc aattctttca agcgctccat tggaacgttg atgtagcgaa
     3361 ctgcacggct accaaccaca ttccccaaca tctcaactgt gtaagatttt ccgtaaggtt
     3421 tatcagcagt tggagcattg ataacagaaa cgtagtcttc taaaggaaga ttgacatatt
     3481 tcttgtaaaa ctcttgtggt gtgattcctt tttcactttt atagttgtta tctttatcgc
     3541 gataagcaaa gtcaaacttg cgtggtggaa gtcctaatga catagcaaga aagttaaaga
     3601 tttcttgcaa gaggtcttct ttcttagctt gaacagtcgc ttgatctgca ccagaaacaa
     3661 gcaaatcacg caagatttga gcatcttgac gaagcaattt attaaggatc gcatttagct
     3721 cacgactgct gctagatgaa acagactcag gataaactga cttaggcacg acaccgtatt
     3781 tttcaaagag ggaaacgacc atatcccatt gaccgccatc ttgttgaggt gtttggagta
     3841 agaagctaac cttgcggcta gtcaattctt ggtctgaagt cgcaatgact tgctccaaga
     3901 accagtttga tttctcatac ttatcccaga agaaagtgtg ggcttgtgac aactcaaagt
     3961 tctccaattt gtattgcgag atgagtttgt ggcggaaggt gttgagagcc gcaaacatcc
     4021 agcaacgacc agacgctttc tggttagtga ccttgtcctt ggttaaatcc aatgagaaaa
     4081 caggtgtgtt gtctacatgg ctttggcgac attccagagc tgcaaaaatt ccgttgtggc
     4141 tggcagcatt ttcaatcgct tggtatttta catttgcttc atagttggca aatagtttat
     4201 cagtaaatga ttcttgaatc gcgttcatag attcctcctt ttattctaca gtctattata
     4261 actcttttca caaaggaagc aactaaaaaa cataaaaggc gaggacattg tccctcacct
     4321 ttttcgaatt tatactcttc gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtac
     4381 tcaagtacag cctgcggcta gtttcctagt ttgctctttg attttcattg agtattaaac
     4441 taaattaaat aatattagcg cggagaattt ctaattcttc cttggtcaag cgacgccatt
     4501 cccctcgttc caggttctca tctaatacta aagttcccat agtcaatcgt tgcaagtcca
     4561 ccacttcctt gccacagtag cccaccatac gcttgatctg atgaaacttc ccttctgcaa
     4621 tggtcacacg gatttggctc tgattctttt ctgcatctgt ggaaagaatc tccagtctag
     4681 cgggctgaca ggtaaagtct ttgagcggaa tgcccttgat aaatgtctcc acatcttctt
     4741 gggtcataat tcccttgacc tgagcctgat aagtcttgtc cacatgacgc ttgggcgaaa
     4801 gaagaacatg agccagctga ccattattgg tcaagagcaa aagaccatgc gtgtcaatat
     4861 ccaagcgtcc tactggaaaa acttccttac tccgcgccaa gtcatccaac aagtccagaa
     4921 cggttctgtg cttgggatcc tcagtcgctg agataactcc tttgggcttg ttcatcatgt
     4981 agtagacaaa ctcttcatac tccaacactt gcccatcaaa gcgaatctca tctatttttt
     5041 catcaatctg caatttagct gatttttctt ttttaccatt tacagtcacg cgcccagcct
     5101 tgagcaagtt tttgacctca gtccgacttc ccaccgcaca ggcaactaaa aatttatcta
     5161 atctcataga actattatat catatcaaaa ggaggctagt acaatgacca acctcctttt
     5221 cgtttcatac tcttcaaaaa tctcttcaaa ccgcgtcaac gtcgccttgc cgtatatatg
     5281 tt
//
LOCUS       DBERCA010000081         5182 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.081, whole genome shotgun sequence.
ACCESSION   DBERCA010000081 DBERCA010000000
VERSION     DBERCA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.081"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(367..1095)
                     /locus_tag="ACL719_001863"
     CDS             complement(367..1095)
                     /locus_tag="ACL719_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0699766.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1253..2662
                     /locus_tag="ACL719_001864"
     CDS             1253..2662
                     /locus_tag="ACL719_001864"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS0699767.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2680..3975
                     /locus_tag="ACL719_001865"
     CDS             2680..3975
                     /locus_tag="ACL719_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0699768.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIRVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3980..4288
                     /locus_tag="ACL719_001866"
     CDS             3980..4288
                     /locus_tag="ACL719_001866"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0699769.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4285..4593
                     /locus_tag="ACL719_001867"
     CDS             4285..4593
                     /locus_tag="ACL719_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0699770.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1745 a          876 c          940 g         1621 t
ORIGIN      
        1 aataaattgg gagattgaaa ttcatttcta gcaatttttt agaagtcaca gtctactatt
       61 ctaacctcag tatactatat gtgttacaag aaaagactca aaaacttctc gtcagtgtaa
      121 gaatgtttga aacaccattt aaaactaaat aactataaga agtagatcag gtgaacaatt
      181 tcagtaccta ctctataagc tatacgaaga tatttttcaa catgatggat aaaacgatca
      241 ctcttttaaa gccatcttat ttagaaaaga gaaaccattt aagcatagat gggaccttct
      301 tgccctgttt taaatttttc ttaaactaga gtctgtaaaa gattggcttc attttcacat
      361 tttctcctag gaggaatgtc gtaatgcaaa agaattaaat tcaaataaat catagcgatg
      421 acgactaatc gagtactcaa acggagttcc gttatccaaa taagccactt gttctacttc
      481 aaataccggt tccgttggaa ggagctgcaa gtaatgttgc tcattttctg aactagaaga
      541 agctcgcaag atttttgtag cactggcaat atgcaatcct aactttcctt caatgtaatt
      601 gtaaatcgat ttttgtaaaa tatcttcagt aatacctgga ataacatcgg tactcatata
      661 agtttgttcc agaacataag gtttaccgtc aataatacgc agacggtaaa tattataaac
      721 tggactctgc aaagcgacct gtagtttttc tgctaaaaat tcatttgcaa attctaattt
      781 gaagtgaata atcctcgagt caattgtaga atgagcatct tcagatattt ttgtcagtcc
      841 ccggatatct ctttctggaa cgattaattt tctttttgag ctgccacgag agagaacaaa
      901 ggttccctgc ccacgttttc taaaaattaa gccctcagaa actaacaagt ctaaagcttt
      961 tttgatcgtc attcgactac aggcaaactg tttagctaat gtcatttcat caggcaattg
     1021 ttcgttcatg gtatatttat tacaaagaat cttttcacga atctcattgt aaataaataa
     1081 atattttgta ctcatttaat ctggttctct ctataatatt ctaatagtag tataacaaag
     1141 ttcaagaaat gaggcaaaca caagatatgt atcaacaatt tttaaaaatg tctagacaaa
     1201 ttttaaaatt atgctatact ttatgtatag aataatagaa aggaggttga gcatgaaaaa
     1261 aaagttagta tttcctaatc tgttttggtg gggagctgct tctagcggac ctcagacaga
     1321 aggtcaatat ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga
     1381 agattttttc gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga
     1441 agatttccac ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag
     1501 tcgactcatc aagaatttag agacaggtga gcctgatcca aaaggtattg ctttctacaa
     1561 tgccatcatt gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt
     1621 tgatttacca gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga
     1681 gttattcgtg aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg
     1741 gacaactttc aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta
     1801 tccaaatcta aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc
     1861 tagtgcaaaa gtgattcaac tatatcgctc attagaactt gatggaaaga ttgggattat
     1921 tttaaacttg acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg
     1981 atttacagat gacttcttta acaaagtctt cttgaatcca gctgttaaag gaactttccc
     2041 agaaagattg gtaaaacagc tagagagaga tggcgtgtta tggagtcata ccgaaaaaga
     2101 gcttcaactg atgaaatcaa atacggttga ttttcttgga gtaaactact accatccaaa
     2161 acgtgttcaa gcacaagcaa atcctgagga atatcagacg ccctggatgc cagaccaata
     2221 cttcaaagag tatgaatggc tggagcgtcg catgaatcca tatcgtggtt gggaaatttt
     2281 tccgaaagcc atttatgata ttgctatgat tgtgaaggaa gaatatggta atatcccatg
     2341 gtttatcagt gaaaacggaa tgggtgttga aaacgaagca cggtttatcg atgaaaatgg
     2401 agttatcgat gacgtgtatc gtattgaatt ttatgaagaa catttaagat ggctacataa
     2461 agccattgaa gagggaagtc actgttttgg ataccacgct tggaccgcat ttgattgctg
     2521 gtcttggaat aatgcatata agaatcgtta cggatttatc tccgttgatt tagaaacgca
     2581 aaagagaacc atcaagagct caggaagatg gtatcgcaaa gtaagtgaca ataacggttt
     2641 tgaagtagaa attgaggagt aaagagagga gatattcata tgaaaaactc aaaatttata
     2701 gaccaatttg ccacctttgc tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga
     2761 gatgcattcg taacagtaat gccattatat attttggcag gtttaatcgt tcttttgaac
     2821 aacacggtat ttaagtggat tttccaaggg gatacattaa caagattcca atattgggga
     2881 ataacaattg caaacggtac tttaagtatt tcaggtatga ttattgctgt aatggttggt
     2941 tatttcttag ctaaaaacag agatttcgaa aacccgttag cagcatcaat gctatcatta
     3001 gtttctttaa ttgtgatgat gccaaataca gtttctgtag ttcctgacgg agcaaaagat
     3061 gcggtaaaca tttcaggtgt tctttcattc aacaacacag gtacaggcgc aatgttcgcc
     3121 ggggttatcg tagcgattat tgcaacagaa ttattcattg aattatcaaa cgttaaagct
     3181 ttacaaatga accttggtga aaatattcca ccagctgtta gtagatcatt tagcgtatta
     3241 cttccagtca tgaccgtcat ctccttattt ggggttgttt cagcattatt attcaatata
     3301 actggaatga acttaatctc aatcattaca atctttattc aagaaccaat tcgtcatatt
     3361 ggtacaagct taatcagggt cattattatt tactctttag gaaatatgtt atggctattt
     3421 ggtattcacc aagcagttat ttacagtgcc atcctagaac cattactatt aattaacatt
     3481 actgaaaaca tcactgcagc aaataatgga caagccattc cacacatcat caacctatca
     3541 caaatacaaa cattcgcttt aatgggtggt agtggatcta cattatgttt attaatagca
     3601 acattcttag tgagtcgcaa tgctgtctct aaaaacgtgg ctaaattatc ttttggacct
     3661 ggtatcttca atatcaatga accagtatta ttcggttacc caatcgttta taacatttca
     3721 ttagctattc catttatcac agttccagtc cttggtattt taatcagcta cttagcaaca
     3781 gttacaggat tcatgagtcc tgcatttata caagttcctt ggactacacc agtattctta
     3841 aatgcatggt tagcaacagc aggggacgtg agagcagttc tagttcaatt catcatcttt
     3901 gcacttggag ttcttctata cattccattt atcaaagtta atgacaaagt tgttgaacaa
     3961 gaaatggaag gttaagatta tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac
     4021 aagtatgctt gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg aaatctctat
     4081 ccaagcaaaa tcaatgacag aagctaaaaa gaatatccat gaagcagatg tcattctaat
     4141 tgggccacaa attcgttatg agttacttgc tgtgaaagaa attgcaggaa atatccctgt
     4201 agataccatt gatatgcggg attacggaat gatgaatgga gcaaaagttc tggaacaagc
     4261 acttgcatgg ataggagaaa taagatgaca gaagaaatgg aatatatctg ttttcagctc
     4321 attgccaata gtggagcagc caaatcatcc tttattgaag ccatccaact agctaaggca
     4381 ggaaatttaa aagaagcgaa aattaaagtt gaagaagcgg aagactcctt agtagaagcg
     4441 cacaagattc actctaatct catacaaaaa gaagctactg gagaaaaaat aggattctcc
     4501 ttgctattta tgcatgcaga agaccaaatg gcttcaacag agattattca gttgctatct
     4561 aaagaattca tcgatctcta tcaaaataaa taggaacact gtcattccca tctgtcttta
     4621 ttaatgacga ctacattgta ggaacctcca aaagttggac aaaatgattg aaaggattgg
     4681 atcctgcatt acacaagatc caatcctttt cttttactct agtttttata atatgttgaa
     4741 tctagaatag tccactgtag tttctaaaaa attgctggaa atggatgtta agctccaatt
     4801 tatttgttta tatcttattt cagtttacta tactagctta ttattctagt gaaaaattga
     4861 atgagccact atttatttaa tatgtatgat gagggaatga ctcaaaaact tcttgtcgat
     4921 ataagaatat ctgagatagt attttaaaac gaaaggacta taaaagctca tttccttcgc
     4981 acttatttta taaagatcta tcattatact ctttagaaat caaaaagcaa cacaccttgt
     5041 catcgtttta tcgtaaatct gttaatgagg gggctagtct tatctgcaat ctcaaatcag
     5101 tgttttgagc tgaatggcac tcgttttcta gtttgctctt tgattttaat tgggccttag
     5161 taaatactac tcttaaatta aa
//
LOCUS       DBERCA010000082         4839 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.082, whole genome shotgun sequence.
ACCESSION   DBERCA010000082 DBERCA010000000
VERSION     DBERCA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.082"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(344..769)
                     /locus_tag="ACL719_001868"
     CDS             complement(344..769)
                     /locus_tag="ACL719_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699771.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(900..1289)
                     /locus_tag="ACL719_001869"
                     /pseudo
     CDS             complement(900..1289)
                     /locus_tag="ACL719_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(1302..1508)
                     /locus_tag="ACL719_001870"
     CDS             complement(1302..1508)
                     /locus_tag="ACL719_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699772.1"
                     /translation="MDLSNKASNLRKKLGADGESSIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(1495..1656)
                     /locus_tag="ACL719_001871"
     CDS             complement(1495..1656)
                     /locus_tag="ACL719_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699773.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(2002..2958)
                     /gene="lytA"
                     /locus_tag="ACL719_001872"
     CDS             complement(2002..2958)
                     /gene="lytA"
                     /locus_tag="ACL719_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS0699774.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(3334..4704)
                     /locus_tag="ACL719_001873"
     CDS             complement(3334..4704)
                     /locus_tag="ACL719_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS0699775.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIAQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         1529 a         1048 c          777 g         1485 t
ORIGIN      
        1 ttgataaaat aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg
       61 acatgcgact gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag
      121 gacttactat ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg
      181 ctcatatgcc tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat
      241 gaatacttcc acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa
      301 gcatattatt ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt
      361 caatcagtat atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa
      421 catctcttag gttattactt cctaatatcc cattatgctt tttcgctaag aaaatagaag
      481 tcgcttctcc cttaccaata atcttgttac tatcatgatt tcttgttaaa tctctatata
      541 atgcgtattc ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca
      601 actggtctat cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct
      661 cttgtggaat aacaatttta cccgaataga gcttttctaa aagatgttca gtgccaaccc
      721 ataaaaatgc tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc
      781 ccctcttcat ctagcccata tacaatatca tatctgaaag catctagtaa cagttcctca
      841 tacttccctt gcgaaattct gttattttct aaaagttgtt cagtcgattt aatatagtgt
      901 cctaatacca tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct
      961 aatcttgaag ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg
     1021 tatccatcat ttctcaatct atataacata gctttatgac tgataccata aaactgaccc
     1081 aattttataa tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca
     1141 accatcctat acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt
     1201 ctttcagttt catctccttc accaatcaag ataagactga ctgaactctt cttcacctca
     1261 tcataataaa gatgatacag tccatgtgct aaagaaaatc tttaccttcc taatggcatg
     1321 tctgaattga ctgcaatgag actgaactga gttcctttat aacagactcc gctaatattc
     1381 tttccgagtc cataaaatac cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata
     1441 tctatcgacg attcaccatc agctcccaac ttttttctaa gatttgaagc tttattactt
     1501 aaatccattc gtgatattcc tttcaccttt ttccattttg gcaatcatgc tttgattcaa
     1561 agacaaatac tcagcaatct tatcttgagt aagatttgaa gatattctaa gttcttttat
     1621 tctttttcca acatcacata catttatcat attcatacac ctctcataaa aagaatagca
     1681 cactcttgtc ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg
     1741 atgctgctta aaaatctaaa attgatgagt tcctatcaaa aatcgtatct tcaacctcaa
     1801 aacagtactt aaagctatcc gactcggttt acattgtcaa atttagattt tatttgagca
     1861 taactttcta gttcgctttc tgatttttgt ttaatatagt agcaaacatt gaaggaaata
     1921 tctccacaag aaaacgcata ctattaagct ttttcaagac ctaataatat gcgctgttct
     1981 gatttgaaag acattccatt attattttac tgtaatcaag ccatctggct ctactgtgaa
     2041 ctctggttta tctgccagtg ttccgtctgg tttgaggtag taccagcctg ttccgtccgc
     2101 tgactggata aaggcatttg ataccatggc gccttcttta gcgtctaagt agtaccaagt
     2161 gtccttgtac ttgacccagc ctgtcttcat ggcaccttct acatcaaaat agtaccactt
     2221 ctcagcgatt ttcttccagc ctgtagccat ttcgcctgag ttgtcgaacc agtaccagtt
     2281 gccgtctgtg tgcttcctcc agcggtctgc aagcatatag cctgaactgt caaagtagta
     2341 ccaagtgcca ttgattttct caaacttgtc ttttggataa gagccgtctg aatgtacgta
     2401 ccagtagcca gtgtcattct tctgccagcc tgtttcaatc gtcaagccgt tctcaatatc
     2461 atgcttaaac tgctcacggc taatgcccca ttttgccaag taagggtatg gatccacatg
     2521 gtctgagtgg ttgtttggtt ggttattcgt gcaatactcg tgcgttttaa ttccagctaa
     2581 gctccctgta tcaagcgttt tcggcaaacc tgcttcatct gctagattgc gtaagagttc
     2641 gatataaagg cggtagtccg tcatgaactc ttctttagtt gaatggcttt caatcagttc
     2701 aaccgctgca taggtctcag cattccaacc gcccccaacg tcccaggcac cattatcaac
     2761 aggtcctacc tgcatgatgc aaccgttccc aacaatgtgc gagaaaaaac ctaattctgg
     2821 gtctttccgc cagtgataat ccgcttcatt ctgtacggtt gaatgcggat tcccagttga
     2881 gtgtgcgtgt acttgcctat atggttgcac gccgacttga ggcaaatctg ttcttaattt
     2941 actcacatta atttccatat tctactcctt atcaattaaa acaactcatt ttttacaatc
     3001 cagaaccaga aactccttta tttctacctt acaaagaaga caatcttagt cacgattaag
     3061 cttgtagata gaacctcaaa acgcactatt ttgacactgt aaataggact gacaaggtct
     3121 gcattctatc tacaataaca ccccagacta aaaagctttt caaaagtata tttttacagt
     3181 ctctatatgt ccttttcata aatactatac tttattatat catataaaag aagtcaaaag
     3241 tctgttaaac tattttcaac accaaactaa agaagagaac acaagttttt tcgatgttca
     3301 ctagaggaaa tggattttat tcagtaaatc caactaggat tgcactttgg ttgccaaaat
     3361 tgcctttcct tcttttatca agggatgacg gaatagtgag aagtacagtt gaactgtcat
     3421 ggcaacccag ataagaggtt cacaaaggat aacaccctta tatcctgccc aaggaataat
     3481 caaaaccaca aaaacgattt ttccgattag ttcaataaag ctagaaacta gaggaaggat
     3541 cttttgcccc aagccctgca agcaattgcg ataaatcaac aagaggctca aaatgggata
     3601 aaaggctgaa ctgatttgca gatagagact tccattttct atcaagtaac catctgtcga
     3661 actagccaag aaggaaacca aggctggact ggcaaaaaag aggaaaatac aaacaaaaac
     3721 tgcccaggat atacttaaac gactgccgat tcgaagacct tgagcaatgc ggtcaggtcg
     3781 cttagctcct agactctgag aagcaaaggt cgtcattgat gcagaaatag cggtcatagg
     3841 aagaagggca aaggtcataa tgcgtcgagc tgccgtctga gcactaataa tcactgcacc
     3901 aaatgtatta acagaagact gtaaaatcac actgccgata gatacaattg aactcatcaa
     3961 gcccatagcc aaaccttgct ccaagagatc cgcgtacaag cttttgtccc atttgaaatg
     4021 tttaaaccgt ggcaagagtt ctggcacact tttacgaata taataaaagc agagaaccgc
     4081 tgataaacct tgtgaaataa tagtagcaag tcctgcggat tgaactccca gatgcaattg
     4141 cgtaataaaa tagagatcca gaaccacatt aaccaaggca gagaaaatca gaaatcccag
     4201 ggctgctaga ctgtcaccaa tagaccgcaa caagcctgca aaaagattat aagcaaagct
     4261 gacacctaca caggtcacaa tcatagaaat atattgataa gattgaggaa gaatttctgc
     4321 aggagtatct aagtattgca agagaggata caagccaaga aagcccagca acataactag
     4381 aatgctcaaa agagcaccta aaatccaggt ggctgctact gcttccttga ttttagtgaa
     4441 attccgagcc ccataataac gagcaatgac aatccccatg ccattgccaa caccaagtgt
     4501 aaaacctaca atcaggtcaa aaatcgctgt cgtcgctcct actgcagcca aggattcttg
     4561 accaagaaat cgtccaacaa tcaagacatc agcagtgtta tagagctgtt gaaaaatatt
     4621 tgatagcaag attggaaagg tgaagcttaa gagcgaggga agaatcggac catgtatcag
     4681 gtccactgtt cgttttttat tcataaagct attatatcag ctttccagag gagcgacaag
     4741 aaacagcaaa cttatatata gcggaatgaa ataaggaaag tcaaatcgat ttttaacaat
     4801 gttttagaag tagatgtgta ctattctagt ttcgatcta
//
LOCUS       DBERCA010000083         4686 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.083, whole genome shotgun sequence.
ACCESSION   DBERCA010000083 DBERCA010000000
VERSION     DBERCA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.083"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..586
                     /locus_tag="ACL719_001874"
     CDS             <1..586
                     /locus_tag="ACL719_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699776.1"
                     /translation="ILKKYKGGAEMPKLLETEKYSLDSIIDGGKLRMISKFCPDLHGL
                     RYEFKTSDSITKEYCKKIRQALRDSDPEGKSGKKCMMRYTIDILNVWNTLCRTRDFIT
                     GSLKADDVIDGKTGIYFFDVNTSNVITDDVIENVKINHKSLVRNVDEENIESISKEIP
                     KGTDMYYYVLYRLGLNRIKYNYLVKALAGAIQKD"
     gene            862..1248
                     /locus_tag="ACL719_001875"
                     /pseudo
     CDS             862..1248
                     /locus_tag="ACL719_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
     gene            1242..1364
                     /locus_tag="ACL719_001876"
                     /pseudo
     CDS             1242..1364
                     /locus_tag="ACL719_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1368..2501
                     /locus_tag="ACL719_001877"
     CDS             1368..2501
                     /locus_tag="ACL719_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0699777.1"
                     /translation="MAIRKGKDGNWVVDISNGFDPITLKQRRLVRKGYMTKKEAIEAE
                     HYLRSVELKERFYGAKITISILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     DNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYSSN
                     PAKLIKKLPIEKTKMQFWTVKEFQKFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSVYVNKGVTHISTTKTKAGTRRIVINKKLSQELQDWEKRQKHL
                     LEQFTNNSLTLQVFQNSPITITKNAIEKQYKKIIERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            2542..2979
                     /locus_tag="ACL719_001878"
     CDS             2542..2979
                     /locus_tag="ACL719_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS0699778.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVASFVTTDEE
                     KAVAYLHDVIEDTTLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKTNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRTKKKS"
     gene            3187..3423
                     /gene="relB"
                     /locus_tag="ACL719_001879"
     CDS             3187..3423
                     /gene="relB"
                     /locus_tag="ACL719_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS0699779.1"
                     /translation="MNNVVTTLISVQFSQELNSLLNAIVEENNTTKTDFIRQAVIEKI
                     EDMYDIKVADEAYKKWVECGKKTISHEEMMRRYG"
     gene            3416..4147
                     /locus_tag="ACL719_001880"
     CDS             3416..4147
                     /locus_tag="ACL719_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS0699780.1"
                     /translation="MAKIYAISDIHGYLDEFIDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEIYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GMLWKELTSSKIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYLI
                     DGDVTNSKTIPVLCYDTITQKYHFL"
     gene            complement(4258..4341)
                     /locus_tag="ACL719_001881"
     tRNA            complement(4258..4341)
                     /locus_tag="ACL719_001881"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4305..4307),aa:Leu,seq:caa)
     gene            complement(4351..4422)
                     /locus_tag="ACL719_001882"
     tRNA            complement(4351..4422)
                     /locus_tag="ACL719_001882"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4388..4390),aa:Gln,seq:ttg)
     gene            complement(4436..4508)
                     /locus_tag="ACL719_001883"
     tRNA            complement(4436..4508)
                     /locus_tag="ACL719_001883"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4473..4475),aa:His,seq:gtg)
     gene            complement(4523..4593)
                     /locus_tag="ACL719_001884"
     tRNA            complement(4523..4593)
                     /locus_tag="ACL719_001884"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4559..4561),aa:Trp,seq:cca)
     gene            complement(4598..4678)
                     /locus_tag="ACL719_001885"
     tRNA            complement(4598..4678)
                     /locus_tag="ACL719_001885"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4642..4644),aa:Tyr,seq:gta)
BASE COUNT         1787 a          728 c          826 g         1345 t
ORIGIN      
        1 aatattaaaa aaatataaag gaggagcaga aatgccaaaa ttattagaga ctgaaaaata
       61 cagtttagat agcattatag atggagggaa gcttagaatg atttctaagt tttgcccaga
      121 cttacatggc ttgaggtacg aattcaaaac atcagactca attactaaag aatattgtaa
      181 aaaaatacgt caagctttaa gagattctga tccagaagga aagagtggaa aaaaatgtat
      241 gatgagatat acaatagata ttttaaatgt atggaacacg ctatgtagaa cacgagattt
      301 tataacgggg agtttaaaag cggatgatgt tatagatggg aagacaggga tatacttctt
      361 tgatgttaat acgtctaatg taattacgga tgatgttata gaaaatgtta aaattaatca
      421 caaaagctta gtacgaaatg ttgatgaaga aaatatagaa agtatttcaa aagaaattcc
      481 taaaggtact gatatgtatt attatgtttt gtatagatta gggttgaata ggattaagta
      541 taattattta gtcaaggctc tggcaggcgc aatacagaag gattaagaat ataattatat
      601 aattaagggg gagttttttc tccccttata gagatagtat tattcttttg gtatttgggg
      661 tatcgtttat caatctcatc ttatagctaa taaaaatagt ctatgttttt agtgaaaaaa
      721 atattatttt aaggagtggt gatatgtata tacttgtcac aaatgggaaa agctacgtga
      781 ctttcccttg gttgcgtgaa atagcttgca gaaattataa aggagaaact aaggtgttta
      841 ctacaaagga actctataat ttcctatatt aaacgcttta actatcacat ttttaatacc
      901 aagaaaagta aattaaaata tatagcagta ttagagaggc aaaaacgtaa agcatggcat
      961 agtcatattt tgctattcag tgttccctat attccacata agcaattatt agaactttgg
     1021 gggcatggag cagtacggat aaataaagtt gacgttgaca gcaaagaaaa tcgcggtcga
     1081 tatgtcacaa agtattttga aaaaggtatt ggacaagaat tgttggaaaa cttcggaaag
     1141 caagcctatt tttcatcacg caacttgaaa aagcctgatg aagataaatt ttacacttat
     1201 gaagatttta actatgatag ttcagtcgta ctatatgaaa cggaatacaa ctgataaccc
     1261 gaggctttac agtctatcag aacaagcgaa ttggtactat acctgctact attgctaccg
     1321 aattactcgg ttttgatgtt caaaacagta ccctcaagga gtaaaatatg gcaattagaa
     1381 aaggtaaaga cggtaattgg gtagttgata ttagcaacgg ttttgaccca ataacactaa
     1441 agcaacgtag acttgtcaga aagggatata tgacgaaaaa agaagctatt gaagcagaac
     1501 actacttacg aagtgtagag ttaaaagaac gcttctatgg ggctaaaata actatctcca
     1561 tactatatga actacttaag gaagaagata gcataaatca tcgaaaagca agctatatca
     1621 atacacaaga taataattac aacaaacaca tcaaagatta tttttctatg gtcgataatg
     1681 tcggaaaact aacctatgaa gacatctacc aatttagaga acatcttagg caaaaagtag
     1741 ctaaaaattc tgataaaccg ttaagcacaa atactattaa caaaatcatg attttactca
     1801 aaaaaatttt tgatgttggt ttgcgaaaag gctattactc ctccaatcca gcaaaattga
     1861 taaaaaagtt gccaattgaa aaaacaaaaa tgcaattttg gacagtcaaa gaatttcaga
     1921 agtttttgac actatttgaa cccgaagaat acaatatcaa actactcttt actgttctat
     1981 tctttacagg attaagactt ggggaagctc ttgctctaac atggcaagac atcgactttt
     2041 caagtaatac aattcatatc acgaaatctg tctatgttaa taaaggagta actcatatca
     2101 gcacaacaaa aactaaagca ggaacaagaa gaattgttat caataaaaaa ttaagccaag
     2161 aattacaaga ttgggaaaag cgacaaaaac atctattaga gcaatttacc aacaatagcc
     2221 taacattaca agtgtttcaa aatagtccta tcacaattac aaaaaatgct attgaaaagc
     2281 agtacaagaa aatcattgaa cgtgatagta cactaaaaaa gatacgcata cacgatttta
     2341 ggcactcaca cgcttccctg ctgattaacc aaggggaaga ttatctagtt gtaaaagagc
     2401 gactaggaca tgcttctata acaaccacga ttgataccta ctctcacttg tacccaagta
     2461 agcaaaagga tttagctgat aaactagatg atttactata aagtaattga gttttataaa
     2521 tggaaagttt ataattaaat catggataca tcacaagtaa taaaagcaca tgaagtagct
     2581 aaaaaagcac actttggaca aattgataga gcaggaattg actacattaa acaccctgaa
     2641 acagtagcta gttttgtcac aactgatgaa gagaaagctg tcgcctatct acatgatgta
     2701 attgaagata ctacactaac cctactagac ctaaaaaagg aaggtttttc aaaaaacatt
     2761 atagaagcag tcgatatact cacaaagaaa aaaggacaag attatcaatc ttatctcaat
     2821 ctagtaaaga caaatgaact tgctagagtt gtaaaattgg cggatttacg gcataattca
     2881 gatttaacca gactaccatt aattactgaa aaagacttag agagaaataa gaaatacagt
     2941 tctgctatta gatttttaag aacaaagaaa aaaagttagt aactaaacta taaaaattta
     3001 taaagagaaa acaaggctta taaactttgt ctttattact atcttggcgg tcagtctcga
     3061 actggcacat ccttgcagat actacaattt tattaaaata aatactatac aaagtgaaat
     3121 tttttattga gaaactttta gactaggagt ataatgtatc taaaataact attaggtggc
     3181 tgttatatga ataatgttgt tactacacta atttctgttc aatttagtca agagttaaat
     3241 tctttattaa atgctattgt tgaagaaaat aatacaacaa agactgactt tattagacaa
     3301 gctgttatag agaaaattga agatatgtat gatattaagg tcgctgatga agcttataaa
     3361 aaatgggttg aatgtggcaa aaaaactatc tcacatgaag aaatgatgag acgctatggc
     3421 taaaatatac gcaatatctg atatacatgg atacttggat gaattcatag acgctctcaa
     3481 taaagtaaat ttaaacgata aagataatcg cctattttta ctaggagatt atcttgataa
     3541 tggacttcaa tcttttcaag tcatttccaa aataatcgaa cttgaggaga tttatcctaa
     3601 tcaaattatt actcttctag ggaaccatga agaatggttt tatgattggt tgattttaga
     3661 taagcctact gcttcagcat ttccagaaac aataaaaagc ttcttctctc cagaggaatt
     3721 gaactatatt tttaaatcga atgctaacaa ctttgaaaca ggtgttagaa atgaaattaa
     3781 aaataatatc aaattcaacc catttataaa ctggtttaaa aagcgatata gggataaacg
     3841 ctattatgaa actgataccc aaatttttgt tcatgcaggc attgatgaag aagcaggaat
     3901 gctttggaaa gaactaacga gttcaaagat atttacaaac aaatttccaa ttaccactgg
     3961 tagatttcat aaagctatta tttcgggcca tattgcttct tgggaagtag ctaaagatag
     4021 aagataccta ggtaagattt actatgatag taaaagtcat tacttaattg atggagatgt
     4081 aacgaatagc aaaacaatac ctgttctttg ctatgataca attacccaaa aatatcactt
     4141 tctataactt ttagtcatgt gcttttatat gaaattagta attattagta acaattccaa
     4201 gaaatttctt atgacataac aaaaagacaa ggcttagaaa ccttgtcttt tcaactatac
     4261 cggcggccgg ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc
     4321 tgccaattcc gccacgccgg caaatagtaa ctggggtagc tggattcgaa ccaacgcatg
     4381 agggagtcaa agtcccttgc cttaccgctt ggctataccc caataatata aaataggcga
     4441 gtgatgggga tcgaacccac gcatgccaga gccacaatct ggtgtgttaa ccacttcacc
     4501 acacccgcca taattctatt aacacgggca gtaggaattg aacccacact gaaggttttg
     4561 gagaccttag ttctaccttt aaactatgcc cgtaaaatgg aaggggaggg attcgaaccc
     4621 ccgaacccga aggagcggat ttacagtccg ccgcgtttag cctcttcgct acccttccaa
     4681 aatata
//
LOCUS       DBERCA010000084         4550 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.084, whole genome shotgun sequence.
ACCESSION   DBERCA010000084 DBERCA010000000
VERSION     DBERCA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.084"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..532
                     /gene="cap8K"
                     /locus_tag="ACL719_001886"
     CDS             <1..532
                     /gene="cap8K"
                     /locus_tag="ACL719_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide polymerase Cap8K"
                     /protein_id="HGS0699781.1"
                     /translation="SIVIFALLYIYMFVENLELRWIGYSFFCISTFLGVLAFQFYPSN
                     NKFSIFIDNILTGRIKLAAYARTFFGYTFWGQYVDKEIVWDPIWGLTSFTFDSFYSFL
                     MSNAGIIWLLILSVLFVKLQKYLDNKSLILLLAWSMYAVTETDLIFPSYGFQFLFLSI
                     LFTNTSTCSTIMLKNN"
     gene            601..1839
                     /locus_tag="ACL719_001887"
     CDS             601..1839
                     /locus_tag="ACL719_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGS0699782.1"
                     /translation="MKIAVTGTGYVGLSIAVLLAQHHQVIAVDIIPEKVELINQRKSP
                     IQDDYIEKYLAEKDLNLVATLDAKEAYKDADFVVIAAPTNYDPKKNFFDTSAVESVIE
                     TVLKYNPDAIMVIKSTVPVGYTESARKKFQTENILFSPEFLRESKALYDNLYPSRIIL
                     GTDKNNERLVSVAETFAGLLQEGALKEDIDTLYMGFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYEDVPE
                     ELIGAIVRSNSTRKDFIADQVLKMVDYYDYSAHNVFNERVEKDIIIGIYRLTMKSNSD
                     NFRQSSIQGIMKRIKAKGVRVVIYEPTLESGSTFFGSSIINDLEEFKRLSNAIVANRY
                     DNSLEDVKEKVYTRDIFERD"
     gene            2415..4397
                     /locus_tag="ACL719_001888"
     CDS             2415..4397
                     /locus_tag="ACL719_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0699783.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVDLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMIPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         1499 a          717 c          902 g         1432 t
ORIGIN      
        1 atctatagta atttttgctc ttttatatat ttatatgttt gttgaaaatc ttgaacttag
       61 atggatagga tactcattct tttgtatttc tactttttta ggagttttgg catttcaatt
      121 ttatccttct aataataagt tttcaatttt tattgataat atcctgactg gacgtattaa
      181 attagctgca tacgcaagaa ccttttttgg ttatacattc tggggtcaat atgttgataa
      241 agaaattgtt tgggatccta tttggggatt aactagtttc acttttgatt cgttttattc
      301 cttcttgatg agtaatgctg gcatcatatg gctacttatc ttatcagtct tatttgtaaa
      361 actacaaaaa tatttagaca ataaaagttt gattttatta ttagcttggt ctatgtatgc
      421 ggtaacagaa actgatttaa tatttccaag ttatggattc cagttcttat ttttaagtat
      481 actttttact aatactagta catgttctac aataatgtta aagaataatt aattgaagga
      541 atctatatat tttctaattt gtagaatgat aggcaaatat atgaggaggg aagaaaaagt
      601 atgaaaatag cagtaacagg tacgggatat gttggcctat ccattgcggt tttattggct
      661 cagcatcatc aagtaatagc ggtggacatt attcctgaga aggtcgagtt aattaatcaa
      721 agaaagtcac ctattcaaga tgactacata gagaaatatc tggcagaaaa ggatttaaat
      781 ttagtggcta cattggatgc aaaagaagca tataaagatg cggattttgt tgtgattgca
      841 gctccaacta actatgatcc aaagaagaac ttctttgaca cttctgctgt agagtctgtc
      901 attgaaacgg tgcttaaata taatccagat gctatcatgg tcattaaaag tacggttcca
      961 gttggttata cagagtcagc tcgaaagaaa tttcagactg aaaatatctt atttagtcct
     1021 gaatttttgc gtgagtcaaa agcactttac gataacttat atcccagccg tattatttta
     1081 gggacagata aaaataatga acgcttggta agtgttgctg agacgtttgc gggattgctc
     1141 caagaaggcg ctttaaaaga agatattgat acattgtaca tgggctttac tgaagctgag
     1201 gcggtcaagc tttttgcaaa tacttaccta gctttgcgtg tttcttattt taatgagctg
     1261 gatacttatt ctgagatgaa ggggctagat actcaagcta ttattgatgg agttggtttg
     1321 gatccacgaa tcggctctca ctataacaat ccttccttcg ggtatggtgg ttactgtctc
     1381 cctaaagata ccaagcaact tcttgccaac tatgaagatg taccagaaga acttatagga
     1441 gctatagtac ggagcaattc gacacgtaaa gactttattg ccgatcaggt tcttaagatg
     1501 gtagattatt atgattattc agcccataat gtttttaatg agagagtaga aaaagatatt
     1561 attattggta tctatagatt gaccatgaag agtaatagcg ataattttcg tcaaagttct
     1621 attcaaggta ttatgaaacg tataaaagct aagggagtaa gagtagttat ttatgaacca
     1681 actttagaaa gtgggagtac attttttgga agttccatta tcaatgattt agaggaattc
     1741 aaacgactga gtaatgccat tgttgctaat cgctatgata actcattaga agatgtcaag
     1801 gaaaaagtct atacacgtga tatttttgaa agagattgat ttagtataat aaaaatgtaa
     1861 aaaattaatt gttagaagcg gtctatactg agaagcaatg ggacgcttag cagattatac
     1921 atattacgat atgcatgttg tgattgaacg agcgctcgaa gttatagaaa aagagtttga
     1981 aaatgacaaa aattagaatc aattggatag attttggaaa agggttctca atatttttag
     2041 ttttagtaga gcgtctatat cctccattcc ccaatttgta gtatgattct aattctgatg
     2101 ttgaaagtgg gaattagctc tactttactt catattgtta tcggaattgt tttgggctgg
     2161 catttatcca tcctagcaac ctatatattg aaaaaaattc catttttgaa tattgtttta
     2221 ttaccacaga agtatattaa attaaaataa attattttgg ctctttgtca gctaacatct
     2281 ggagaggaca atcaccgtct tctccttttt ggtcttttca gaatatacta aactaaccca
     2341 aaaattctga aaattctgtt gacatctttt tgaaaagagt ctataatgga gagaaagttt
     2401 taaaggagaa aatgatgaaa agttcaaaac tatttgccct tgcgggcgtg acattattgg
     2461 cggcgactac tttagctgca tgctctggat caggttcaag cgctaaaggt gagaagacat
     2521 tctcatacat ttatgagaca gaccctgata acctcaacta tttgacaact gctaaggctg
     2581 cgacagcaaa tattaccagt aacgtggttg atggtttgct agaaaatgat cgctacggga
     2641 actttgtgcc gtctatggct gaggattggt ctgtatccaa ggatggattg acttacactt
     2701 atactatccg taaggatgca aaatggtata cttctgaagg tgaagaatac gcggcagtca
     2761 aagctcaaga ctttgtaaca ggactaaaat atgctgctga taaaaaatca gatgctcttt
     2821 accttgttca agaatcaatc aaagggttgg atgcctatgt aaaaggggaa atcaaagatt
     2881 tctcacaagt aggaattaag gctctggatg aacagacagt tcagtacact ttgaacaaac
     2941 cagaaagctt ctggaattct aagacaacca tgggtgtgct tgcgccagtt aatgaagagt
     3001 ttttgaattc aaaaggagat gattttgcca aagctacgga tccaagtagt ctcttgtata
     3061 acggtcctta tttgttgaaa tccattgtga ccaaatcctc tgttgaattt gcgaaaaatc
     3121 cgaactactg ggataaggac aatgtgcata ttgacaaagt taaattgtca ttctgggatg
     3181 gtcaagatac cagcaaacct gcagaaaact ttaaagatgg tagccttaca gcagctcgtc
     3241 tctatccaac aagtgcaagt ttcgcagagc ttgagaagag tatgaaggac aatattgtct
     3301 atactcaaca agactctatt acgtatctag ttggtacaaa tattgaccgt cagtcctata
     3361 aatacacatc taagaccagc gacgaacaaa aggcatcgac taaaaaggct ctcttaaaca
     3421 aggatttccg tcaggctatt gcctttggtt ttgatcgtac agcctatgcc tctcagttga
     3481 atggacaaac tggagcaagc aaaatcttac gtaatatctt tgttccacca acatttgttc
     3541 aagcagatgg taaaaacttt ggcgatatgg tcaaagagaa attggtcact tatggggatg
     3601 aatggaagga tgttaatctt gcagattctc aggatggtct ttacaatcca gaaaaagcca
     3661 aggctgaatt tgctaaagct aaatcagcct tacaagcaga aggtgtgact ttcccaattc
     3721 atttggatat gccagttgac cagacagcaa ctacaaaagt tcagcgcgtc caatctatga
     3781 aacaatcctt ggaagcaact ttaggagctg ataatgtcat tattgatatt caacaactac
     3841 aaaaagacga agtaaacaat attacatatt ttgctgaaaa tgctgctggc gaagactggg
     3901 atttatcaga taatgtcggt tggggtccag actttgccga tccatcaacc taccttgata
     3961 ttatcaaacc ttctgtagga gaaagtacta aaacatattt agggtttgac tcaggggaag
     4021 ataatgtagc tgctaaaaaa gtagatctat atgactacga aaaattggtc actgaggctg
     4081 gtgatgaggc tacagatgtt gctaaacgct atgataaata cgctgcagcc caagcttggt
     4141 tgacagatag tgctttgatt attccaacta catctcgtac aggtcgccca atcttgtcta
     4201 agatgatacc atttacaata ccatttgcat tgtcaggaaa taaaggtaca agtgaaccag
     4261 tcttgtataa atacttggaa cttcaagaca aggcagtcac tgtagatgaa taccaaaaag
     4321 ctcaggaaaa atggatgaaa gaaaaagaag agtctaataa aaaggctcaa gaagatctcg
     4381 caaaacatgt gaaataactg ttgcaaaata taagaaagga tttagtattt ctcttgaatg
     4441 ctgaatcctt ttttacattt gtaaagaaag attctaaatg tactgacccc caaaagttgg
     4501 agcctctttt tgtcagaata gagaaaattt ttgttaattt tacttgtttc
//
LOCUS       DBERCA010000085         4130 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.085, whole genome shotgun sequence.
ACCESSION   DBERCA010000085 DBERCA010000000
VERSION     DBERCA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.085"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            67..312
                     /locus_tag="ACL719_001889"
     CDS             67..312
                     /locus_tag="ACL719_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699784.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFTKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            713..1705
                     /locus_tag="ACL719_001890"
     CDS             713..1705
                     /locus_tag="ACL719_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699785.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1707..2525
                     /locus_tag="ACL719_001891"
     CDS             1707..2525
                     /locus_tag="ACL719_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699786.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2527..3312
                     /locus_tag="ACL719_001892"
     CDS             2527..3312
                     /locus_tag="ACL719_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0699787.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3351..3629
                     /locus_tag="ACL719_001893"
                     /pseudo
     CDS             3351..3629
                     /locus_tag="ACL719_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
     gene            3566..3811
                     /locus_tag="ACL719_001894"
     CDS             3566..3811
                     /locus_tag="ACL719_001894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0699788.1"
                     /translation="MSRRKKAYQGRKIGSQLLATLESEAHKKVGYLQVKTVAEGSNKD
                     YDRTNDFYRGLGFKKLEIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1155 a          696 c          891 g         1388 t
ORIGIN      
        1 attatggtat cgaatcttca tcagaatgat aaaattaatc aattaatatc tgattataaa
       61 cagaatatga aagcttttta tatcactatt gaaaaattta tacgagatga tgaaagcctt
      121 aagtgttatt ttacaaaggt tatttcaagt cgttccaagg taacaagtct agatcagatt
      181 gaagctgata aaacgataca aagaaaatat tcaagtgagc taaaaaaatt tattggattt
      241 tataatgaga ttatttgtga ggaaaatagt ttcctacatg tacgaaagag gtggtcgagt
      301 tggtttaggt agtcgatgcg tgagttgata attctcaggg tatggacttc tttttcatga
      361 atgaggtaaa agagcaggta ttgtttagag acaatcattc tgagcatatt ttctggatag
      421 agggagtatc cgattttatg atcaaagtta ataccgccct ctggtgagaa gatgagtagg
      481 ttggtaattt aaactattaa acagaatttt tgattaaaag tattatttta tgagagaaat
      541 cctaatttca caatccatag gcaaacgctt gcattctgtt ttttattgga ctataatagg
      601 ttggtataaa gccttctgta gtaataaaat gtagaaggtg tagaaagtaa ggatttagaa
      661 tatttgtagt taaaaataca atgttgctat tccttatgat agggagatag atatggcaat
      721 gatagaagtg gaacatcttc agaaaaattt tgtgaagact gttaaggaac cgggcttgaa
      781 gggggctttg cgctccttta ttcatcctga aaagcagacc tttgaagcgg tcaaggattt
      841 gacctttgag gttccaaaag ggcagatttt aggatttatc ggggcaaatg gtgctgggaa
      901 gtcgacaacc attaaaatgc tgacaggaat tttgaaacca acatctggtt tttgtcggat
      961 taacggcaag attccccaga acaatcggca agattatgtc aaagatattg gcgtagtctt
     1021 tggacaacgc acccagctat ggtgggattt ggctctgcaa gagacctaca ctgtcttaaa
     1081 agagatttat gatgtgccag actcgctctt tcataagcgt atggactttt tgaatgaagt
     1141 cttggatttg aaggacttta tcaaggatcc cgtgcgaact ctttcactgg gacaacggat
     1201 gcgggcggat attgcggcct ccttgctcca caatcccaag gttctttttt tagatgagcc
     1261 gaccattggt ttggacgttt cggttaagga taatattcgt cgggcaatta ctcagatcaa
     1321 tcaagaggaa gaaactacca ttcttttgac cactcatgat ttgagtgata ttgagcaact
     1381 ttgtgatcgg attttcatga ttgacaaggg gcaagagatt tttgatggaa cggtgagcca
     1441 actcaaggag acctttggta agatgaagac tctctctttt gaactgctac caggtcaaag
     1501 tcatctcgtc tctcactatg aaggtctgtc tgatatgacc attgatagac aaggaaacag
     1561 cctcaacatt gaatttgata gttctcgcta ccagtcagct gacattatca agcaaaccct
     1621 gtctgatttt gaaatccgcg atttgaagat ggtggatacg gatattgagg atattatccg
     1681 tcgcttctac cgaaaggagc tctaggatga tcaaattgtg gagacgttat aaacccttta
     1741 tcaatgcagg ggttcaggag ttgattactt accgagtcaa ctttattctc tatcggattg
     1801 gcgatgtcat gggggctttt gtggcctttt atctctggaa ggctgtcttt gattcttcgc
     1861 aagagtcttt gattcagggc ttcagtatgg cggatatcac cctctacatc atcatgagtt
     1921 ttgtgaccaa tcttctgact agatccgatt cgtcctttat gattggggag gaggtcaagg
     1981 atggctccat tatcatgcgt ttgttgcgac cagtgcattt tgcggcctcc tatcttttca
     2041 ccgagcttgg ttccaagtgg ttgattttta tcagcgttgg ccttccattt ttaagtgtca
     2101 ttgtcttgat gaaaatcata tcgggtcaag gtattgtaga ggtgctagga ttaactgtcc
     2161 tttatctttt tagcttaacg ctcgcctatc tgattaactt tttctttaat atttgctttg
     2221 gattttcagc ctttgtgttt aaaaatcttt ggggttccaa cctacttaag acttccatag
     2281 tggcttttat gtcggggagt ttgattccct tggcattctt tccaaaggtt gtttcagata
     2341 ttctctcctt tttgcctttt tcatccttga tttatactcc agttatgatc attgttggaa
     2401 aatacgatgc cagtcagatt cttcaggcac tccttttgca gttcttctgg ctcttagtga
     2461 tggtgggatt gtctcagttg atttggaaac gggtccagtc ctttatcacc attcaaggag
     2521 gttagtatga aaaaatatca acgaatgcat ctgattttta tcagacaata catcaaacaa
     2581 atcatggaat ataaggtgga ttttgtggtt ggtgttttgg gagtctttct ggctcaaggc
     2641 ttgaatctct tgtttctcaa tgtcatcttt caacatattc cattcctaga aggctggacc
     2701 tttcaagaga tagctttcat ttatggattt tccttgattc ccaagggaat ggaccatctc
     2761 ttttttgaca atctctgggc actagggcaa cgcctagtcc gaaaagggga gtttgacaag
     2821 tatctgactc gtcccatcaa tcctctcttt cacatcctag ttgaaacctt tcagattgat
     2881 gccttgggtg aactcttagt cggtggtatt ttattgggaa caacagtgac cagcattgtt
     2941 tggactcttc caaaattcct gcttttccta gtttgtattc cttttgcgac cttgatttat
     3001 acttctctta aaatcgcaac agccagtatc gccttttgga ctaagcagtc aggcgccatg
     3061 atttacatct tctatatgtt caatgacttt gctaagtatc cgatttctat ttacaattct
     3121 cttcttcgtt ggttgattag ctttatcgtg cctttcgcct ttacagccta ctatccagct
     3181 agctatttct tacaggaaaa ggatgtgttc tttaacgtag gaggtttgat gttgatttct
     3241 ctggttttct ttgttatttc ccttaaactt tgggataagg gcttagattc ctacgaaagt
     3301 gcgggttcgt aaaagctaaa gtaagactaa aatcaagaaa ggaacttatg atgtttgtaa
     3361 ttgaagaagt caaggatgaa aatcaaaaaa aggcagttgt cgctgaggtt ttgaaggatt
     3421 tgccagaatg gtttggaatc ccagaaagca cataagccta tataaaagga accacgacac
     3481 tgcaagtttg gaccgcctat caggagagtg atttgactgg atttgtaagc ttatcctatt
     3541 cgagtgaaga ttgtgcagag attgattgtc tcggcgtaaa aaagcttatc aaggtagaaa
     3601 aattgggagc caattgcttg ctactttaga gagtgaagct cataaaaaag ttggttatct
     3661 gcaggtcaaa acagtggcag aaggttctaa taaagattat gatcgaacaa atgactttta
     3721 tcgaggtctt ggctttaaaa agttagagat ttttcttcaa ctatggaatc cgcaaaatcc
     3781 ttgtcagatt ttgattaaaa agcttgaata atattacttg acatctattc tcagagtgct
     3841 atactgtaag tgtaatcgcc gatttagctt agttggtaga gcaaggcact cgtaaagcct
     3901 aggttatagg tagataaacg actgaggatt tgaaaaaata gataggtaga agataaccgt
     3961 taagccttac tcttagcggt tatttatatt gtttaatagc gctaatattt tatcaattat
     4021 gcctgttttc gtgtttctgg tagttgttca agtttattgc taatattttt gatggtatga
     4081 atgtgcttat aatgtatccc ggttaacgaa agttttggac ttatactctt
//
LOCUS       DBERCA010000086         3908 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.086, whole genome shotgun sequence.
ACCESSION   DBERCA010000086 DBERCA010000000
VERSION     DBERCA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.086"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            155..382
                     /locus_tag="ACL719_001895"
     CDS             155..382
                     /locus_tag="ACL719_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699789.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            670..3567
                     /gene="nanA"
                     /locus_tag="ACL719_001896"
     CDS             670..3567
                     /gene="nanA"
                     /locus_tag="ACL719_001896"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGS0699790.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1338 a          641 c          865 g         1064 t
ORIGIN      
        1 ttaaaaataa ggtataataa atttactaac acttataaaa agtgatagaa tctatcttta
       61 tgtatattta aagatagatt gctgtaaaaa tagtagtagc tatgcgaaat aacagataga
      121 gagaagggat tgaagcttag aaaaggggaa taatatgata tttaaggcat tcaagacaaa
      181 aaagcagaga aaaagacaag ttgaactact tttgacagtt tttttcgaca gttttctgat
      241 tgatttattt cttcacttat ttgggattgt cccctttaag ctggataaga ttctgattgt
      301 gagcttgatt atatttccca ttatttctac aagtatttat gcttatgaaa agctatttga
      361 aaaagtgttc gataaggatt gagcagggag tatggtataa atagcataag ctgatgtcca
      421 tcatttgctt ataaagagat attttagttt aattgcagcg gtgtcctggt agataaacta
      481 gattggcagg agtctgattg gagaaaggag aggggaaaat tggcaccaat ttgagatagt
      541 ttgtttagtt catttttgtc atttaaatga actgtagtaa aagaaagtta ataaaagaca
      601 aactaagtgc attttctgga gtaaatgtct tatttcagaa atcgggatat agatatagag
      661 aggatcagta tgaatcggag tgttcaagaa cgtaagtgtc gttatagcat taggaaacta
      721 tcggtaggag cggtttctat gattgtagga gcagtggtat ttggaacgtc tcctgtttta
      781 gctcaagaag gggcaagtga gcaacctctg gcaaatgaaa ctcaactttc gggggagagc
      841 tcaaccctaa ctgatacaga aaagagccag ccttcttcag agactgaact ttctggcaat
      901 aagcaagaac aagaaaggaa agataagcaa gaagaaaaaa ttccaagaga ttactatgca
      961 cgagatttgg aaaatgtcga aacagtgata gaaaaagaag atgttgaaac caatgcttca
     1021 aatggtcaga gagttgattt atcaagtgaa ctagataaac taaagaaact tgaaaacgca
     1081 acagttcaca tggagtttaa gccagatgcc aaggctccag cattctataa tctcttttct
     1141 gtgtcaagtg ctactaaaaa agatgagtac ttcactatgg cagtttacaa taatactgct
     1201 actctagagg ggcgtggttc ggatgggaaa cagttttaca ataattacaa cgatgcaccc
     1261 ttaaaagtta aaccaggtca gtggaattct gtgactttca cagttgaaaa accgacagca
     1321 gaattgccaa atggtcgagt tcgcctgtat gttaatggtg ttttatctcg aacaagtctg
     1381 aaatctggca atttcattaa agatatgcca gatgtaacgc atgtgcaaat cggagcaacc
     1441 aagcgtgcca acaatacggt ttgggggtca aatctacaga ttaggaatct cactgtgtat
     1501 aatcgtgctt taacaccaga agaggtacaa aaacgtagtc aactttttaa acgctcagat
     1561 ttagaaaaaa aactacctga aggagcggct ttaacagaga aaacggacat attcgaaagc
     1621 gggcgtaacg gtaaaccaaa taaagatgga atcaagagtt atcgtattcc agcacttctc
     1681 aagacagata aaggaacttt gatcgcaggt gcagatgaac gccgtctcca ttcgagtgac
     1741 tggggtgata tcggtatggt catcagacgt agtgaagata atggtaaaac ttggggtgac
     1801 cgagtaacca ttaccaactt acgtgacaat ccaaaagctt ctgacccatc gatcggttca
     1861 ccagtgaata tcgatatggt gttggttcaa gatcctgaaa ccaaacgaat cttttccatc
     1921 tatgacatgt tcccagaagg gaagggaatc tttggaatgt cttcacaaaa agaagaagcc
     1981 tacaaagaaa tcgatggaaa aacctatcaa atcctctatc gtgagggaga aaaggaagct
     2041 tataccattc gagaaaatgg tactgtctat acaccagatg gtaaggcgac agactatcgc
     2101 gttgttgtag atcctgttaa accagcctat agcgacaagg gtgatctata caagggtgac
     2161 caattactag gaaatatcta cttcacaaca aacaaaactt ctccatttag aattgccaag
     2221 gatagctatc tatggatgtc ctacagtgat gacgacggga agacatggtc agcgcctcaa
     2281 gatattactc cgatggtcaa agccgattgg atgaaattct tgggtgtagg tcctggaaca
     2341 ggaattgtac ttcggaatgg gcctcacaag ggacggattt tgataccggt ttatacgact
     2401 aataatgtat ctcacttaaa tggctcgcaa tcttctcgtg tcatctattc agatgatcat
     2461 ggaaaaactt ggcatgctgg agaagcggtc aacgataacc gtcaggtaga cggtcaaaag
     2521 atccactctt ctacgatgaa caataaacgt gcgcaaaata cagaatcaac ggtggtacaa
     2581 ctaaacaatg gagatgttaa actctttatg cgtggtttga ctggagatct tcaggttgct
     2641 acaagtaaag acggaggagt gacttgggag aaggatatca aacgttatcc acaggttaaa
     2701 gatgtctatg ttcaaatgtc tgctatccat acgatgcacg aaggaaaaga atacattatc
     2761 ctcagcaatg caggtggacc gaaacgtgaa aatgggatgg tccacttggc acgtgtcgaa
     2821 gaaaatggtg agttgacttg gctcaaacac aatccaattc aaaaaggaga gtttgcctat
     2881 aattcgctcc aagaattagg aaatggggag tatggtatct tgtatgaaca tactgaaaaa
     2941 ggacaaaatg cctataccct atcatttaga aaatttaatt gggacttttt gagcaaaaat
     3001 ctgatttctc ctaccgaagc gaaagtgaag cgaactagag agatgggcaa aggagagatg
     3061 ggcaaaggag ttattggctt ggagttcgac tcagaagtat tggtcaacaa ggctccaaca
     3121 cttcaattgg caaatggtaa aacagcgact ttcctaaccc agtatgatag caagaccttg
     3181 ttgtttgcag tagataagga agatatcgga caagaaatta ttggtatagc taaaggaagc
     3241 atcgaaagta tgcataatct tcctgtaaat ctagcaggtg ccagagttcc tggcggagta
     3301 aatggtagca aagcagcggt gcatgaagtt ccagaattta cagggggagt taatggtata
     3361 gagccagctg ttcatgaaat cgcagagtat aagggatctg attcgcttgt aactcttact
     3421 acaaaagaag attatactta caaagctcct cttgctcagc aggcacttcc tgaaacagga
     3481 aacaaggaga gtgacctcct agcttcacta ggactaacag ctttcttcct tggtctgttt
     3541 acgctaggga aaaagagaga acaataagag aagaattcta aacatttgat tttgtaaaaa
     3601 tagaaggaga tagcaggttt tcaagcctgc tatctttttt tgatgacatt caggctgata
     3661 cgaaatcata agaggtctga aactactttc agagtagtct gttctataaa atatagtaga
     3721 ttgaaataag atgtgaacaa ctctatcagg aaagtcaaat taatttatag aattatttta
     3781 gcagtcaagg tgtactgtta tagattcaat atattatatg actattaacc ttgtcttctc
     3841 ctaaaattga ctttcttgtt ttcttatctt gtccactcga aacaagtatt gtaagaattt
     3901 gattattt
//
LOCUS       DBERCA010000087         3794 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.087, whole genome shotgun sequence.
ACCESSION   DBERCA010000087 DBERCA010000000
VERSION     DBERCA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.087"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..418
                     /locus_tag="ACL719_001897"
     CDS             <1..418
                     /locus_tag="ACL719_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGS0699791.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="ACL719_001898"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="ACL719_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGS0699792.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1420 a          625 c          744 g         1005 t
ORIGIN      
        1 taaaaataag gcagaccaag atagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaattcagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggccga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaa
//
LOCUS       DBERCA010000088         3710 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.088, whole genome shotgun sequence.
ACCESSION   DBERCA010000088 DBERCA010000000
VERSION     DBERCA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.088"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            488..868
                     /locus_tag="ACL719_001899"
     CDS             488..868
                     /locus_tag="ACL719_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HGS0699793.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSADAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGVDWADGSLDGYIFA"
     gene            870..1076
                     /locus_tag="ACL719_001900"
     CDS             870..1076
                     /locus_tag="ACL719_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699794.1"
                     /translation="MKHFFAGIGEINFVSYLLYICVGIAPFFHVYIIGSEMNFVKIVL
                     SILGIIFVCILTIARIYRTFFYKE"
     gene            complement(1269..2132)
                     /locus_tag="ACL719_001901"
     CDS             complement(1269..2132)
                     /locus_tag="ACL719_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0699795.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRRIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            2598..2936
                     /locus_tag="ACL719_001902"
     CDS             2598..2936
                     /locus_tag="ACL719_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS0699796.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
     gene            2975..3409
                     /locus_tag="ACL719_001903"
     CDS             2975..3409
                     /locus_tag="ACL719_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699797.1"
                     /translation="MGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINYIKV
                     GGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDLMVY
                     IFLGYVLVIQKDVYLFSILIIFSNFSVPFIREKEYELFKNKK"
BASE COUNT         1191 a          460 c          757 g         1302 t
ORIGIN      
        1 tatgtaggta gaaaataaag ataggagaaa aagtatgaat aaaaaaatat tttgtattct
       61 agtatgtatt atatcattga ttagtctagc tataatcttt ccatggggct ggtcgatata
      121 atgtaaggga gcttgagttg ttagtggagg ataaaatgtt aagagtaata gtagttattc
      181 aatctttagt aataatactg cttgctgttg ctttattatt atctcggaaa aactttgatt
      241 cgttaaatgt tgttcttata atgatgatcg tatcacaatt gttgttatta gtatatttga
      301 taaaatcagg gaatcagtga ttagctgtta tttgaattaa gtaaactgtt aagcttcaag
      361 cagtaactgg ttttaaatat ttaaaataag ataaaggaag gaagtctaat ttaggtaact
      421 aaacaaagcc tgcgatcact tgttaggaaa ccatcctaac catgcttaat aaaaaaggag
      481 aaaataaatg gaactcgtat taccaaataa ttatgttgct cttgagcaag aagagatgat
      541 gtatcttgat gggggtggtg ttggtcgtaa ctggtggaat agtagaggta gttttgcaac
      601 agttctggat gtagatttgg ccatctatag tggtggtgca acaatttatt ctgctgatgc
      661 gataaaaaaa gctatctcag ctaatagagg ggctattacg agaacattac gtagtttaat
      721 aattaaacat gtaggtagtg cagctggcca tttagtcaat actgcactaa acgttgcact
      781 aactgttact ggattttcac taggtggagc aatcgcatat ggggttgatt gggctgacgg
      841 tagcttagat ggttatattt ttgcttaaaa tgaaacattt ttttgcagga attggtgaaa
      901 taaactttgt aagttattta ttgtatatat gtgtaggaat agcccctttt tttcacgtat
      961 atataatagg ttctgaaatg aattttgtga aaatagtgtt atctattttg ggaattatat
     1021 ttgtttgtat tttaactatt gctcgaattt ataggacttt tttctataaa gaatagtttg
     1081 gacttgaaat gtatttaaaa acaagaggtt ggtagattat cagcctcttt cttgtcgttg
     1141 aaaaagtgag atatattgac ttttgaaaaa aatgtcataa ttgagttggg ataaacgtaa
     1201 aagtcatact ttctcgttaa atcgttcggc gattctatga aaaggtcgag gagctccctc
     1261 gacttttttt ataatttctt tggttgataa tgggtctcta cttctttttt gagatgagac
     1321 agcatagaag tttcctcggt cagctctagg agtgagaaac ctttttggat aagtttagca
     1381 ttatctgtac aaatcccaat acggacatag cagatagcaa gcctatcgta gcttttctta
     1441 ttcttggcgt cctccagtaa agtgtagcag atttgcttac acatgttttt atcttgattg
     1501 tatagataga gtgtggaaag gttgagcagg attgctattc gcaagtcata taaatgttga
     1561 tagtttttat agacttctag gcgttgcaat atttttccag tgatgagatg gaggtattca
     1621 atgggaaaac tgaaaagaat ggtattgagg atttttagat cactctcata ccatgtatct
     1681 tgtttttcaa ttcttctcca aagttttttg acaacctgtt ctgcgtgatc tgatagttcc
     1741 cctgccccgt gttgacggat ataaatgaca acttcaagca tatctctgat ttcttctata
     1801 gggagatcgt ggtgggtctt gaggtagtct tggcattttt gaaataaatt gacaagatcg
     1861 ctggtcccga taatggaacg catattgaga taggtttgca taatctctgt gcgttgactt
     1921 ggttgataaa gttgacagat atattcaaac tcttcaaaac tcatattgat ttgacggaga
     1981 agaaattcca tattttcata ttttggggtt gtcttgccac tttcgatttt tgataagctg
     2041 gttcttgaga gaacaccccc acagacctct tcttgggtca gcccctttga ctcacgtatt
     2101 tctttataga cctttccgaa atcataacgc atgattttct cctttctgtg aaaaaagtta
     2161 acaaataata aaatcttgtt aaaaatattg tatcatagca gtgtaaaaag taaaaaagaa
     2221 agtaaaaata aatttgtgaa aaaagttaac gtaattttaa aatgagctta ataagaggta
     2281 tgatgtaggt agaaaataaa gataggagaa aaagtatgaa taaaaaaata ttttgtattc
     2341 tagtatgtat gatatcattg attagtctgg ccataatctt tccatggggc tggtcgatat
     2401 aatgtaaggg agcttgagtt gttaaaggag gcttagtcct tggacaaaaa tctgctgtac
     2461 tacaatgctt ggaaagatat caagaacaaa gaatgatacg attgaaacta aactgattca
     2521 agaactactg ggcagtgatt gaggtttggg tgacatcaaa gaaaagttag attaattcta
     2581 aagaattgag tatttctatg ttaaaaaaat atccttgtac gatgcaacat gatcagtcag
     2641 attgtgctgc ggcagttgtt tcaacagttc ttttatctta caaaaaggaa ctatcaatca
     2701 tgaagattcg ggaaattatt ggtacagaca tgtatggaac gactgtcagt ggtattgttt
     2761 caggtctgaa taagttgaat tttacagtaa aagctgttcg agtagcactg gaagatttga
     2821 ctccaaaatt aacatttcct gcgattcttc aagttaagaa tgatttaggt caaaatcatt
     2881 ttgtggtatt acatagtata aaggagaaaa taaagggaac tcgtattacc aaataattat
     2941 gttgctcttg agcaagagga gatgatgtat cttgatgggg ggggcttatt taagcaagag
     3001 tgcttgtcaa ggaatttgcg cagctttagc tatgagtcca ggaactttta tagcattgac
     3061 tggagctgca gttttaacaa aaaaactaat aaactatatt aaggttggag gccttggagg
     3121 ttggcttatt ggtgcagcag caggtgtatt ggctcttatc tttttgataa aaatttgtgt
     3181 agatactacg ataaaggttc tattaaacag aatctttaag gcaagtaagg taatgaaaag
     3241 gaggatattt tctctttttg tactagattt gatggtctat atatttttag gttatgtttt
     3301 agtgatacaa aaagacgtat atctgttttc aattctgata attttttcta atttttcagt
     3361 tccctttatc agagaaaaag agtatgaatt atttaaaaat aagaaatgag atagatatgg
     3421 tagctttatt aaaactgaga ataaagtttg ggagatattt ttatttattt taggtgtgtt
     3481 gttaattgta atttcattac taagtccccc aaaaattaaa ttttggaaat agagttagtg
     3541 agaaggatag gataatagta ttatgatgat tgttttaatt tcagttattg ttgcattcct
     3601 gtatcataaa attacagagt atgtcaacaa caggaaaatt gatattgtta tttttctatc
     3661 attgctgatt tctcatttta tttggaatta ctatttttta ccaattgaaa
//
LOCUS       DBERCA010000089         3569 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.089, whole genome shotgun sequence.
ACCESSION   DBERCA010000089 DBERCA010000000
VERSION     DBERCA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.089"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            89..1168
                     /locus_tag="ACL719_001904"
     CDS             89..1168
                     /locus_tag="ACL719_001904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699798.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSEIERVYANLWTVA"
     gene            1113..2090
                     /locus_tag="ACL719_001905"
     CDS             1113..2090
                     /locus_tag="ACL719_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699799.1"
                     /translation="MSFILKLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDL
                     AILWSYQQLAKKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEF
                     GEIDRAVELFTTHFIEIFENSQNKNLYSGQVSIDFSFKSPFRVLLTGTSGSGKTTILN
                     LINGSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQII
                     EVLKKVNLYEELEKIDILNYQCGENGSNLSGGQKQKIALARALIRNNKVYLFDEISAN
                     LDNDNSNSIHDILFNLGISFIEVSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            2105..2845
                     /locus_tag="ACL719_001906"
     CDS             2105..2845
                     /locus_tag="ACL719_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699800.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            2848..>3569
                     /locus_tag="ACL719_001907"
     CDS             2848..>3569
                     /locus_tag="ACL719_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS0699801.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLS"
BASE COUNT         1235 a          450 c          688 g         1196 t
ORIGIN      
        1 gaatccaaag gattctccct ttttatttaa ataagtctgt ctagctatag atatattaag
       61 tttgataaaa tttcttggag attttgttat ggattataat ttcaatttgg agcatccttt
      121 ctttttcact aataatgatt atagtacaga tactagcata aagtaccaag taagtctgcc
      181 ttttaattgg cacgaagtaa tgaataatga tgagtgggtt tatcaatacc ctattggaaa
      241 atttgttgaa agacaagggt ggaaaataca cattagttca gagtataaca gttcacatga
      301 attgttacaa gatgtagcaa aaatttgcca tgaaatgcga attccattta aacatcttag
      361 tacagaagac aaatttatca tgcgaaacgg gaaattggtt agtcgaggat tttctggtaa
      421 atttattaca tgttatccaa atcaaaatga attagagtcg gttttgcaaa ggttagaaag
      481 tgccttaaag caatataatg ggccatatat tttaagcgat aaacgttggg atgaagcacc
      541 aatatatctg aggtatggtg tgtttaggcc gtcaagggat gatgaaaaga aggttgctat
      601 tgatgaatta attgttggcg atgaagttgt taaagatgaa cgtttaccag tatttaaaat
      661 tccaaaaggt atagtgcccc ctgacttttt gaataaatgg cttgacaaga aagataaaaa
      721 acagggagac tttcccttta ttatagataa tgcaattaga ttttcaaaca gtggtgggat
      781 atataatgct aggctaaaag aagatgggaa aaaaataatt ttaaaagagg ctagaccgta
      841 tacaggtcta ggatttgatg gtacttattc ttcagagaaa ttagcttcgg agtgcaaagc
      901 actaaaaatt ctaaatgaat ggagtgagac gcctaaaata tactggcacg gtaagatctg
      961 ggagcacacc ttcttaggaa ttgaacatat gaagggcgtt ccactaaata gatgggttac
     1021 caataatttt cctttatatg aagttgttga taagaccaaa gattatctat tgagagtttc
     1081 aaagatagta gaaaaactca tcgatttaac gaataagttt cattctgaaa ttgagagggt
     1141 ttacgctaac ttatggacag tggcgtaaag aatttcaatc aagattcttc ccagttcatt
     1201 attatggtct gaattttaat caaggaattg cgttttcaga tttagctatc ctttggagtt
     1261 atcaacaact agctaagaaa gtaaaaaatt tcaagtttga tgactattat gagattcgaa
     1321 cacaggttat taatgaggca gtaaataatt ttaagaaatc gtccctttct ggtctttttg
     1381 atggtaaaat tggtacaatc tggctcattt atgaatttgg ggagatagat cgggcagttg
     1441 aactttttac aactcatttt atagagatat ttgagaattc acaaaataaa aatttatata
     1501 gtgggcaggt aagtattgac ttctcattta agtcaccatt tagggtatta ttaacaggaa
     1561 cttctggcag tgggaaaaca acgattttaa atttaattaa tggttcttta aagccacaaa
     1621 aaggttatgt aaatttgtta tcacatggga aaaagagttc agattcaata ccaacagttg
     1681 atcagacacc atatattttt gacactacta ttcgtgagaa cgtaacttta tttcaaaatg
     1741 aatatttttc agatgatcag ataattgagg tgttaaaaaa ggtaaatcta tatgaagaat
     1801 tagaaaagat agatatacta aattatcaat gtggtgaaaa tggtagtaat ttgtctggag
     1861 gtcaaaaaca aaaaatagct ttagctagag ctctgattag aaataataaa gtgtacttat
     1921 ttgacgaaat atcagctaat ttagataatg ataattcaaa ttccatacat gatattctgt
     1981 tcaatttagg tatttcattt attgaagttt cacatcatta tgacttaaat gacaagagat
     2041 acactgatat atataaattg gaaaatggaa cacttttcaa aattaagtga ttggaggcaa
     2101 gaatatggat aaagattata tattaaaagt gaaagggctg tatcatcaat ttttactagg
     2161 aaataataaa acgttgcaag tgctgaaaaa tgtttctctt tctgcttcga gaggagaatt
     2221 tataagtatt ctaggaatta gtggttctgg aaagtcaact ttattaaaat gtatttctag
     2281 tttgcttgaa ccaacaagtg gggaagtaat tttaaatgga atcaacccct ataaaatcag
     2341 aaatgcaaaa ttgtcaagta taagacgtaa cgaagtatct tttatatttc aagcatacaa
     2401 tttaatacct tccctgccgg taatagaaaa tatagcactt cctttgcgat tatcacaaaa
     2461 aaaattaact attaaaaatg tagaaaactt actcaaaaga atgaagttta atgctggctt
     2521 aaacgatttt gttggaactc tgtctggtgg agaacaacag aaggttgcta tagctagagc
     2581 ggttattgct gatagtgata taatatttgc tgatgagcca actggggctt tagacagcgt
     2641 ttctcgtgaa gtaatttttg aattattgag agagttagta ggggcgggta agtgtgtaat
     2701 tatggtaacg cacgatatag aattggcctc gaaaactgat cgtgcattaa tattgaaaga
     2761 tggaaaaatt ttcaaagaac ttcatagacc tagcggggaa gagttgtata aaatcttaga
     2821 ggtacaatca actacggagg aatagagatg ctcagattag tttactatca atttttacat
     2881 aataagaaac aatggttagg cgtttcacca gtaatttttg tttcgagttt agttatggga
     2941 ttggctgtta atggggtaat taatgttgag aataattcgc aagtatttgt cggacttcca
     3001 gatcctaaac caatttttat gtttccgata gtattcggag gagtaacatt attctttgtg
     3061 ctctctaaca taataaatat gttggttgaa atttttagag atgattacga acttttggag
     3121 gtgttgggag caagtcgcct tcaactatca tttcttgtgg ggggacagat ttttatcatt
     3181 tcctcgatta ttagttttat tgcatatcta tgctctatat ttgttacgtc aaattattat
     3241 tattttttac aatacttttt cggggaaaat atattacctg atatccaatt tcaaacgagt
     3301 gctgttggat gtatcattac agtagtgttg atttcatttt tagcctttct aagtggttgc
     3361 ttttatacgt tcaagaagat tagaaatagg aagagttcta aaataaggca tgttctttca
     3421 atagtaaaaa gaatactgct attggctggt tttagtgtta tctggttgct ttctttgcaa
     3481 caaattttcc aagatagcac aattttagcg aaggcccaga taatattcaa tattgtaatc
     3541 ttagatattg tgattattta tcaattatc
//
LOCUS       DBERCA010000090         3445 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.090, whole genome shotgun sequence.
ACCESSION   DBERCA010000090 DBERCA010000000
VERSION     DBERCA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.090"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(170..424)
                     /locus_tag="ACL719_001908"
     CDS             complement(170..424)
                     /locus_tag="ACL719_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGS0699802.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(444..2285)
                     /gene="typA"
                     /locus_tag="ACL719_001909"
     CDS             complement(444..2285)
                     /gene="typA"
                     /locus_tag="ACL719_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGS0699803.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDSTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2447..3172
                     /locus_tag="ACL719_001910"
     CDS             2447..3172
                     /locus_tag="ACL719_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0699804.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            complement(3212..3439)
                     /locus_tag="ACL719_001911"
     CDS             complement(3212..3439)
                     /locus_tag="ACL719_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699805.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
BASE COUNT         1036 a          757 c          639 g         1013 t
ORIGIN      
        1 tcaaaataat atgcaaccag tactaaccaa atataaaata gatgccatta aagaatttta
       61 ttcaagtttt tcccattcat attatccaaa caaaagagat ggtgataact caaaaataaa
      121 ttgcaatctt gtaaaattcc tagcaaaaaa gagagccaaa actctctttt tatcttcttt
      181 tacttttttt gactggcatg agtgtgatgt ctctaacact aaagtaagct aggatcaaca
      241 tggctattgc taggaatatt tctgttggta attgaaaaat tttcagaaaa gatagaacca
      301 ataaaatcaa gagtgccact aaaatacata ccatagcgac gatattgaca gtccctttaa
      361 tgctttctgg tgtcgcaaat acatagagta ggagcagtaa aattcctagg actaaataga
      421 ccatctttct ctctttctag ctcttattca gctgattttt tcttcttgtt agctttctca
      481 cgctctgctt tgttaaggat ttgtttacgc aaacggatag actcaggcgt tacttccatg
      541 tactcatcgt cgttcaagaa ctcaagagac tcttcaagtg tcaagatacg aggcgtcttg
      601 ataacagctg tttggtcctt agtagctgaa cgaacgttgg tcatttgttt tgccttcgtg
      661 atgttaactg tcaagtcatt ttcacgagag ttttcaccga tgatcattcc ttcataaacc
      721 tcagtacctg ggttgacaaa gatcgtacca cgttcttcga tagacatgat tgagtaagtt
      781 gtagccttac cagcatcgat agaaacaagg gcaccacggt gacgtccacc aatttcccct
      841 ggaatcaatg gcaagtattg gtcgaaggta tggttcatga taccgtaacc acgagtcatt
      901 gacaagaact cagttgagta tccaatcaaa ccacgcgctg gaacaaggaa gaccaaacga
      961 gtttgaccat taccagttga aatcatatcc aacatttcac ctttacgttc agaaaggctt
     1021 tggataacag acccttggta ttcttctgga gtgtcgattt gtacacgttc aaatggttca
     1081 catttaacac cgtcgatttc ttttacgata acttctggac gagatacttg aagttcatag
     1141 ccctcacgac gcattgtttc gataaggatt gacaagtgca attctccacg tcctgaaaca
     1201 gtccatttat ctggtgaatc agttgagtca acacgaaggg aaacgtctgt ttgcaattct
     1261 gcctgcaagc gttcttccac cttacgagaa gttacccatt taccttcttt accagcaaat
     1321 ggtgagttgt tgaccaagaa agtcatttga agagttggct catcgatgtg taggattgga
     1381 agagcttcta ctgcatctgt cggagtgatg gtttcaccga caaagatgtc ttccatacct
     1441 gaaacggcaa tcaagtcacc cgctttggct tcttggattt cacgacgttc caaaccaaag
     1501 aaaccgaaga gttttgtaac acggaagttt ttagttgtac cgtcaagttt agaaagggta
     1561 acttggtccc caaccttaac tgtaccacgg aagacacgac cgataccgat acgtccaacg
     1621 aagtcattgt agtccaaaag tgacacttgg aactgcaaag gctcatctga gttatctact
     1681 ggagctggga tatggtcgat aatcgtgtca aagattggtg ccatagtcgc ttcttggtca
     1741 gctggatcat ctgacaatga agaagttccg ttgatcgctg aagcataaac cactgggaaa
     1801 tcaagctggt cgtcatctgc accaagctcg atgaaaagtt ccaagacttc atccactact
     1861 tctgctggac gagctgatgg cttatcgatt ttgttaacaa ccacgattgg gacaaggtct
     1921 tgttccaagg cttttttcaa tacgaaacga gtttgtggca tggttccttc ataggcatct
     1981 acgaccaaga caacaccgtc aaccattttc atgatacgct caacttctcc accaaagtcc
     2041 gcgtgtcctg gtgtgtccat aatattgata cgagttccgt tgtaagcaac ggcagtattt
     2101 ttagcaagga tggtaattcc acgctctttt tcgatatcgt ttgagtccat agcacgctct
     2161 gccaattcag tccgtgcatc aagcgtttct gattgtttca ataattcgtc aaccagggtt
     2221 gttttaccgt ggtcaacgtg ggcgataatc gcaatgttac ggatatcttc tcttaatttt
     2281 gtcatgattt cctctataat attcaaaatt tattttctaa ctgaacgatt ataccataat
     2341 ttcaagtaaa taacataact caagcaagtg taaatgtttt cactccgctt ttcttttcac
     2401 gtcaagcctt ttcaaagcaa gcgacttatg ataagatagg cacagtatgc gtttagataa
     2461 tttattagcc caagaaaaag ttagccgaaa ggccatgaag caagcactcc tcagagggga
     2521 aattctagtc gatggttgcc cagcccgctc cctagctcaa aatatcgata caggactaca
     2581 agaactcctt tttcaggatc gaatcattca aggctatgaa cacacctatc ttatgcttca
     2641 taaacctgct ggtgtcgtta cagccaacaa agacaaggaa cttccgaccg tcatggacct
     2701 gcttccatct gacatccagt ctgacaagct ctatgccgtt ggccgactgg accgagatac
     2761 aacgggactc ctcctcttga ccgataacgg tcccttgggc tttcagctcc tccatcccca
     2821 atatcatgtc gataagactt accaagtcca ggttaatgga cttctaacac ctgaccatat
     2881 ccaagccttt caaaagggaa ttgtcttttt agatggtact gtctgtaaac ccgcaaaact
     2941 agagattcta tctgcaagtc cctccctcag tcaagcctct atcaccattt cagaaggaaa
     3001 atttcatcaa atcaagaaaa tgttcctctc ggttggtgtt aaggtgacta gcctcaaaag
     3061 aatccaattt ggggacttca cattgaaccc agatttagca gaaggtaact accgcccttt
     3121 gaaccaaaaa gagttacaaa tcattaaaaa ctatttagag atgagtcgat aaaacaaaaa
     3181 aagctttaaa actaaagctt ttttctttta tttaccgaaa aattaaggcg attgctacaa
     3241 tccagttaac tacagaaatc acaattccta agatattaag aatcttttct attttatagt
     3301 ctaattgtga ctctttttgg tatgaaatag ccaagaccaa tcctatgata cccaaaatca
     3361 ggcctacaat tggaaataac aaaccaagaa taatcgacaa gatacccaca aaaagtggat
     3421 ttttcttctt ttctttcatg ttcta
//
LOCUS       DBERCA010000091         3261 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.091, whole genome shotgun sequence.
ACCESSION   DBERCA010000091 DBERCA010000000
VERSION     DBERCA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.091"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..170
                     /locus_tag="ACL719_001912"
     CDS             <1..170
                     /locus_tag="ACL719_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0699806.1"
                     /translation="EYENRTVSFAISSKGENISYRVDHRILFDNISFDTSSSGVTLIT
                     GKNGTGKSTLL"
     gene            complement(292..458)
                     /locus_tag="ACL719_001913"
                     /pseudo
     CDS             complement(292..458)
                     /locus_tag="ACL719_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(536..1846)
                     /locus_tag="ACL719_001914"
     CDS             complement(536..1846)
                     /locus_tag="ACL719_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGS0699807.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2083..2955
                     /gene="nadC"
                     /locus_tag="ACL719_001915"
     CDS             2083..2955
                     /gene="nadC"
                     /locus_tag="ACL719_001915"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGS0699808.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3086..>3261
                     /locus_tag="ACL719_001916"
     CDS             3086..>3261
                     /locus_tag="ACL719_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS0699809.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGIKPR"
BASE COUNT         1019 a          549 c          733 g          960 t
ORIGIN      
        1 aagagtatga aaacagaact gtttcttttg ctattagttc aaaaggagaa aatatttcgt
       61 atagggtgga tcatcgtata ttgtttgata atatttcttt tgatacttcg agttcaggcg
      121 tgacattaat tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa
      181 ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac tatcctgatc
      241 tatttgtcct gttcttattt catttcacta catctcaaat tgagtatgac gaagtgcgct
      301 cccatgtcct gggaacgcac tttcttcata tttttcatat tcttgaatcc atcgataaag
      361 actattggga tgaattttta aagttgaact aatcattttt acaggatgag gtttacagca
      421 gagagtttga aggctttatc aaaggttttt cttggcataa tgacttttcc tcgtttccac
      481 ttaattttgt gtctacttta ttataccaag tccaccctta agttagataa taaatctaac
      541 ttaaggaagc tagaaggatg agaatccagg tggtcaagag tcccaaactt aagctgatgg
      601 ggacacccag aataatttgc tttttgaagg caaggccacg ttcctctata ttgggaagtg
      661 agagttgaat gagagaacca gctgatgaaa agggtgagat attagtagat agagcgccaa
      721 taacggtggc tgttgtgagt aagtgaatat caatctgagg attttgagca ctgatgatag
      781 caatgatggg aaagagggct ggagctacaa cggatagggt ggaactaaag agtgacatca
      841 ctccggctat cacacaaaag aacagaggta accagaaatg aggaatggat gttgtcatga
      901 ggtgccctat cagtgtgact aaacctgact tgaccgctag agacattagt aagctcatgc
      961 cgcagagcat gataattgta gcccagggaa ctttagccaa aatggcttct tgcttcccta
     1021 atttgagcct taaggcgagg cagaccatga gtattgagac aaagccaata tcaaatgttt
     1081 tttgataagt agctatccag gcgatgtttg ggaaaatgag atgcaacaag ggaaaaagcc
     1141 aaaccaaaac catgctgctg atcatgagca aggtggtttg tctttgaacc ttgctgagga
     1201 gtggtggttg gtcaatagtc aaggatgagt ttgttcttcc cttactatag tgactgtaac
     1261 aggataataa aagcaagacg atgagtgggt agataatgct gacgataaag atatgattgc
     1321 caagtgaaaa agcttgctct tcccatccca tttgcttaaa caggccttga aagacaatgc
     1381 ctgagctact ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg gcttgagctc
     1441 caatcaaagg gtgtttgtcc gctttttgac agagggtaat cgctagagga cagcaaacgg
     1501 ccatagtagt gaaaaatcca gcacctaaag cagacaaaag ggttgccatc aggtataaaa
     1561 tcatgtagag ggcgttaggg taggtgcgtg tgcggtagag aatgtgttga gccaaaacat
     1621 caagagtacc gttagttatt gcaaagttat aaaagagaga gacgctaaaa atggtaaaaa
     1681 agagtgaggt tggccaaaaa tgaagaagtt ctttggggct taatcccatg agagtggttg
     1741 cgatgaggta agaaaaagca atagccagca ggccaatatt gattttggtg cggtaaccaa
     1801 ttccaatggc tagagcaatg gcgctaatca ttattaaatg aatcattgaa ttgtcctttc
     1861 agttagaata tggaaatagg ttggcaattt tggcgctcaa aagaccacct gtttcctatt
     1921 ggtacttgac tattatacac aaaaaaaata caattgtctt gacaattgta ttgacaattg
     1981 ttagaattaa aaatgaggta ttcaggcttg tgatttccta tggaagttaa tagtgattgc
     2041 ctctaatgct tacaagtgat attaaaaata gaggacctag tgatgtcaat catttcaact
     2101 gatttaaccc cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat
     2161 tccgaagatt acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt
     2221 tttgccaagg aagctggtgt tttagcgggg ctaaccgttt ttcaaagggt ttttacccta
     2281 tttgatgccg aggtgacctt ccagaatcct catcaattta aggatgggga tcgtttgact
     2341 agtggcgatt tggttttaga aatcataggc tcggtgagaa gtctcttaac atgtgaacgc
     2401 gttgccttga attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta
     2461 gaagccttag gcgatgattg cattaaggta tttgatactc gaaaaactac tcctaattta
     2521 cgtctttttg agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta
     2581 tcagatgcta tcctgctaaa agacaatcac attgcggcag taggtagtgt tcaaaaggca
     2641 attgctcaag cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagc
     2701 cttgctgctg ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt ggataatatg
     2761 tcattggaac agattgaaca ggccattacc ctaattgcag gacgttctcg gattgaatgt
     2821 tctggaaata ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc
     2881 tccagtggta gtttaaccca tagtgctaag agtcttgatt tttccatgaa ggggttaacc
     2941 taccttgatg tctaagttgt aaaataaact aactttttaa aggatgtctt tcctctagaa
     3001 cgagttttat gtcagatagt ttaaacgcct cttcaaatat agtaaaatga accaaaaata
     3061 gtacacaatg tggtataatc tttttatggc atattcaata gattttcgta aaaaagttct
     3121 ctcttattgt gagcgaacag gtagtataac agaagcatca cacgttttcc aaatctcacg
     3181 taataccatt tatggctggt taaagctaaa agagaaaaca ggagagctaa accaccaagt
     3241 aaaaggaata aaaccaagaa a
//
LOCUS       DBERCA010000092         3066 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.092, whole genome shotgun sequence.
ACCESSION   DBERCA010000092 DBERCA010000000
VERSION     DBERCA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.092"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(148..618)
                     /locus_tag="ACL719_001917"
     CDS             complement(148..618)
                     /locus_tag="ACL719_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0699810.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(635..2908)
                     /locus_tag="ACL719_001918"
     CDS             complement(635..2908)
                     /locus_tag="ACL719_001918"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGS0699811.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT          898 a          546 c          688 g          934 t
ORIGIN      
        1 aaggcgacgt tgacgcggat tgaagagatt ttcgaagagt ataaatcttc ctaggataga
       61 gcaaaacgca tagtatcaag ggttttctga tgttaaagac tttctaccag gtttttttaa
      121 agcataattg ttagttgtag tcatttatta ttcttcaaag aaaaatggtg gggcgaattt
      181 tttcagttct tcaaagcact tttgagcagt atctgcatct tcacagatga taagacagac
      241 atcattacca caaagggtag cgatagcgtc agggaagctc aaagtatcaa tgatagaacc
      301 aaaggattga gccagtccag gaagggtttt tagtaggact tggtgttgaa ctgggcgcat
      361 ccagacaagg gcgtcttcca tgtagagttc gagacgtttt tcccattttg agatggaacc
      421 attgttaaga acataataag cgctatcttc ttcgcggact tttgataggt tcatattttt
      481 gatgtcgcgt gagagggttg cctgggttac ttgaatgtcg ttctcagcaa gaagggcttg
      541 caactcagcc tgtgtatgaa tcttgttttt tgtgataaga gcgcgtataa gttggtggcg
      601 gtgttctgat ttattcataa taatgtaact ccttttagca aggtaaggta agcatggact
      661 gagcgaggtc gacagtcaag tggtagtctg tattgtcacg gatggtgatt tcaaagtcag
      721 tagtatagag gactaaacgg agagtgtctc cttcttttag cttgtaaata gttggctgca
      781 gttcaaattg aacgtccatc cattcatctg cagtaatatc ctctactaac agtaaatcat
      841 ttctattttg taaattaagg taaccttttg tcacgactcg ttgtgcctct ggtctaaatg
      901 gcaattcaca gagattttcc aacatgtgat agcgaccgtt atcaatggtt ctagcactta
      961 aaatagctgg ataaggttgt aggtatttct tttgcccaaa ttctagcagt tgggcagata
     1021 agagcccctt gtttgtactg gatttgatac gaagattgag ctgagcgcga ccgtttaggt
     1081 ggagatcttt agtcacagga aggttaatag taatctgatt ggctttccct tgatagagct
     1141 ctgtattgaa ggtttggtat gtcttaccat agcgctcaaa atccttatct gggtactggt
     1201 tttgaatagc ttgctcttct tgaccaagtg agaaggtttc acagttttct tgcccaccga
     1261 agttatcaag tgataaccaa gtctgtggag ctgtattgtc ctgccagata acagtaggaa
     1321 gttgaaagtc tgtttcctgt cctagtaatt tcttggtcaa taaggcattt atggactcac
     1381 ggaagtcaat tgattgccaa ttgttcatgt aaacatgagc accattatgg aaaaagagat
     1441 gcttgtgtat atgagtagga agagcatgga acatctggta aacatgaagt ggtttgacat
     1501 tccaatcctg agaaccatga gtaaagacaa cctctgcctt tactttatgg gcattgagca
     1561 gataattgcg gtcatgccaa aactgattgt agtccccagt ttttcggtct agctgagctt
     1621 tcactttttc taagtcagct tggtgagctt cattgccacg gatatagtcg ccagctaaga
     1681 gattacgaga ataggttaac tcagcaaggg agtcaaagtc ctcacctgga taaccacctg
     1741 ggctagtcac cagaccgttt tcacggtagt agttgtacca tgatgaaatt cctgcctcgg
     1801 caatgataac ttctaaacca tcgactcctg tagtcgcaag accattggac atggtaccta
     1861 gataggaaag tcctgttgta gcaacttttc cgtttgacca atcagccttg acttgacgct
     1921 ggcgcgtgtg atcagtaaag gcacggcaac ggccgttaag ccaatcgatg acatttttat
     1981 aagcctcgat ttgctggtag tctccattag tcatgaaacc tgtcgagtct ttggtaccaa
     2041 cacctgagac atagagattg gcaaagcctc tcggaaggaa gtagtcgttt agtgtatagc
     2101 tagagttgat gtgagttagc ttttcctcag cctctgctat aagctcagct ttaccttggg
     2161 gttggacgag atttagttga ggtttctcta gctcaatctt gtgaggaagc ttaacctcaa
     2221 gctcgccctc catcttgtag agagccttgt cactagcctt gtcattggtt ccctgatgat
     2281 aagggctggc tgtcatgatg gcagggattt ttccatcaaa acgagggcga ataatgctaa
     2341 cctttactag gtctgatagc cctttttggt cagtatcgac acgagactca acgtaaacga
     2401 cttcacgaat gacatcctgg ttagaaaaag tagccaaact cttgccgtta aagtagtggt
     2461 agtcattatc ctccggaata agaccatcac taacaagttg gtcgataaga gtatttcctt
     2521 ttttggtgcg agtattgagt aactgataga gattttcaat caagtcacca tatataatgg
     2581 gaaatccagt ttctttacga aaaacgtcac tatcttcgaa gtcaaccaaa taagaaaagc
     2641 ctaaaagttg aaaagcaaca gtataaaaaa tatctgctgt cagttcatct tctgattgaa
     2701 aaaatgtcag caggtctgtt tttttatcag ctgctaggat agaaagtggg tagttggtgt
     2761 cttgataagt gaaaaagaaa cgacgtaaaa aggtttcaag tgagtctttg tgattggctg
     2821 tattttgtaa atcaaagcca cattttttta gttcagataa gacattttct tttggaaaat
     2881 tgatataact atattgatta aaacgcatag aacctccata tagaatgaca gttaaggtta
     2941 ttatatcaaa aaaaaagcag aaagggaatt gttaacttca aaaggaaata atccaataaa
     3001 aatgaataaa gtactaaatt caatatagag aacagagtaa caataagaat aaatagatag
     3061 ggtata
//
LOCUS       DBERCA010000093         3028 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.093, whole genome shotgun sequence.
ACCESSION   DBERCA010000093 DBERCA010000000
VERSION     DBERCA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.093"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..338
                     /locus_tag="ACL719_001919"
     CDS             <1..338
                     /locus_tag="ACL719_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HGS0699812.1"
                     /translation="SGKLKKLLSDKEKLSPKKWNEEKILLMSNLEEINKEKDKIKDEY
                     QEINHIKYSVDFVNKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDNEYREMM
                     KNKKMDQER"
     gene            412..585
                     /locus_tag="ACL719_001920"
     CDS             412..585
                     /locus_tag="ACL719_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699813.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            771..1304
                     /locus_tag="ACL719_001921"
     CDS             771..1304
                     /locus_tag="ACL719_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HGS0699814.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKEKVKPNKKDMTREI"
     gene            1431..2159
                     /locus_tag="ACL719_001922"
     CDS             1431..2159
                     /locus_tag="ACL719_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGS0699815.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWY
                     EEDKDKLRQKGLNKKMNYDVGESL"
     gene            2843..3004
                     /locus_tag="ACL719_001923"
     CDS             2843..3004
                     /locus_tag="ACL719_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699816.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT         1439 a          391 c          519 g          679 t
ORIGIN      
        1 taagtgggaa attaaaaaaa ctcctatcag ataaggaaaa actaagtcct aaaaagtgga
       61 atgaagaaaa aattctttta atgtcaaatc ttgaagaaat aaataaagaa aaagacaaga
      121 taaaagatga ataccaggaa attaatcata taaaatattc agtagatttt gtaaacaaag
      181 aacttggtat agatttatcc atagaaatag ataagctaat aaaacaggga gaaaaaccaa
      241 gtgtaatagc acagattaaa aagttccaag accaagtcaa taaagataat gaatacagag
      301 aaatgatgaa aaacaagaaa atggatcaag aaagataaga cctggtaaaa atatcttatc
      361 caagctataa tatgaccaaa caactaaagt cagatctagg aggtaataga gatgaataaa
      421 aggcaagagc taatagatga actcataaaa gcaaatgaag atggaacata caaaacctat
      481 aaaagcacag aagaaataaa agcaatgaac aatgaagaaa tacagattat atattctaac
      541 atgaaaaact atctatcaga caaaagaaca cacataaact attaaaggtg gaaaggtagt
      601 gtaagctatt tttaaaagta taaaaggtac aaagacctaa gaaatatata aaaacatcaa
      661 aataaagtat tccaccacca agaacaataa aataaaaaca agcaaaggga agtatagaaa
      721 aattaaaacc tatacttccc ttttttaatt caaacaaagg agagaaaagc atgataaacg
      781 aagaaataag caaggaagca ggtcaagcag cacaaaccat aatatcatac acaataaagg
      841 caacaaaaga atcaatcaat ttagaaaaag aaataagaaa aaagatgaat gaaactttag
      901 aaaaagcaaa tggaaactta aaaagtctta tgggcgatga aatgaaaata aaagacctct
      961 acaagaaagg acaactagaa aatataagca tagatcaaat cgacctcaaa gacttaaaaa
     1021 aagaactaaa caaacttgga gtaagtttct cagtaatgaa aaacaaagaa agcaaaaact
     1081 atgaaatatt cttccaagcc aaagacataa aagtaatgga atatgccttt aagcaagtca
     1141 tagccaagga aaataaaaaa gaaaaagaaa gtatcctaaa acaaataaag aaatacaaag
     1201 acctatccaa aaacaaagat aagacaaaag aaaaaggaaa aaggaaagta aaagaaaaag
     1261 aaaaagtaaa accaaacaaa aaagatatga ccagagaaat ctaaagggag taacgaaaag
     1321 ttacaccctt aaataaccat aataacaaga cttccgaaaa acagaagtcc agaattccga
     1381 aaatcggaaa tcaagaattt cgattatcga aaatcaagaa aggagcagat atggcaacaa
     1441 acaaaagata ctattggata aaattaaaag aagaattttt cacagacaaa aggataaaaa
     1501 ggttaaggag aatatctgga ggagatacct atacgataat ctacctaaaa cttctactac
     1561 taagcctaaa agatgagggc aaactctatt atgacggagt agaaagtgat tttataaaag
     1621 agttagcact aaccatagat gaaacagacg atgatgtaat ggtaacagta aattatttaa
     1681 tcaatcaagg cttattagaa gttgtaacag aaaatgatga atactaccta actgaaatac
     1741 caaacttaat cggatcagaa acagcctggg cagagaaaaa aagaagatac agacaaaata
     1801 aacagaggac attgtcctta atgagtccaa cccatgtccg acaagagata gagatagaga
     1861 aagatataga gatagataaa gagagagagg gagagataga taaaaagtcc acccccattt
     1921 tttatggaga attcaaaaat gtaaggttaa gcaaagaaga atataaaaat ctaaaagaaa
     1981 aattaaactc acacacagat ataatgataa ataaactatc cagatacatg gaaagcagtg
     2041 gtaagaccta tcaaaaccac tatgtgacaa tcttaaaatg gtatgaagaa gataaagaca
     2101 aactaagaca gaaaggttta aataaaaaaa tgaattatga tgtaggagaa tctttataga
     2161 taaaaagagg tggaaaggca ctattaaaga ctttaagttt aaaaaggtat caaagtatgc
     2221 cagagataaa aaaatataaa tatgacactt taaaggcgat tagaaaatta aagaaatgct
     2281 agaagacttt tagatgacag ttaccaagag ggtctaattt tcactgctac ttatgcagta
     2341 ggaattggac ctttttatat aagcaaattc tatttcaaaa ttggttacca agaaagactt
     2401 cttttcactg ttaagtataa gagggtaatt tatagaattt tgttacatca agataaatat
     2461 tttctgatgg tcaaatttta aaaagattaa taactctcga taaattttga accttaacaa
     2521 ttgaatagac caagacaaga cgttaattat ttttggagcg taataaccta tccgtcaaga
     2581 tctttcggct gaataattcg gcaaaaaaag ttctgctaag caaaaagaaa gtgagcgata
     2641 agtaagagtt tgaatatagg gagggatacc ctatccgcta tgaagaaaat gaggataatg
     2701 atacttctaa ggttgaagca ggctcatccg gaatagaggc ggaggtgaga ttcctatgtt
     2761 gctaatccaa agcacttgtc aatgtcaaaa aacttaatta aacaaataat tattagatat
     2821 aaaactttag aggaggtata tattggacaa tgactggaat ggattggctg atttaatagc
     2881 aaatctgatt gcaaaatatg ctggtgcttt agatttagat aatctacccg atccaacgcc
     2941 agcaaaaaat caagagatga aaaatagttt tgacatggca aaaacacaaa ttgagacgga
     3001 ctaataaaag tgttataata tacttgaa
//
LOCUS       DBERCA010000094         2822 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.094, whole genome shotgun sequence.
ACCESSION   DBERCA010000094 DBERCA010000000
VERSION     DBERCA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.094"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            75..1253
                     /locus_tag="ACL719_001924"
     CDS             75..1253
                     /locus_tag="ACL719_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699817.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLMFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLGRLVI
                     "
     gene            1462..>2822
                     /locus_tag="ACL719_001925"
     CDS             1462..>2822
                     /locus_tag="ACL719_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS0699818.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALPEAVVTDKG
                     EPAV"
BASE COUNT         1009 a          493 c          578 g          742 t
ORIGIN      
        1 agtgttttga gctgactgcg gctagctttc tagtttgctc tttgattttt attgagtatt
       61 agaaaggaga cctcatgaca cttccagtta gaaaatccct gcacgatgca gttttacagg
      121 cttcgaaagc ggatacttgg gaacaagcta ccaaggaatg gaatgaagtt tccttgatgt
      181 ttaacggtat cggccgtagc aattgtgtct gtggaaatgc catcaaatac gcttatgaac
      241 tctttaacgg tgtcacaggc caacgcctct ttccaattgg tagcgactgt gttcgtcatt
      301 ttcatcgatt gtcccttgac cagcaattgg aagaggaaga aaaactgctc agaaaggttg
      361 aaaatctaac cagaaaagct cagaaaaagg aaaaaatcaa ggtcaataaa agtgactttg
      421 acgagcgact tctaaaatgg ctctgggaaa aaggtgtttt caaacccaat cgtggcaatc
      481 aatttgcgcc tgagagagac taccagcttt tcctagaagt ctttcaggga ggaagttgga
      541 ccaaggcgga accaaagaaa aaggctcgga tggaagaagt ccttgaaaag tgtatcaaac
      601 catttttact tgggaaatcg gatgaccaac tctaccttgt caagctaggc aaggagaaaa
      661 ttgactacga acaagaactc cgtatccagg cagagaaaga acgcaagaag agggataaaa
      721 tcgccaagca atatgcagat aatctggttc tagccatggg acccgcagag cgtgcttatc
      781 aagattactt tggctttaca gagaccctga ctcaagaaga acgcaagtgg gaaaaaattc
      841 tttttggtaa aaatagagca gaacgggcta tcaaggccaa acaataccaa aaagagctgg
      901 aaaaagacca acgaattgcc agtcaggatc caattgaaag aaaacagaag cagacttggc
      961 tcctcaattc ctattttcga gagcttcctg aagaaaaagc tcgattttct aggctcttat
     1021 tagaatatcg aaaaagtggt gaagtaccct tttcaactga atacctgtca gatcatctca
     1081 tcgacttttt ctataaaatg aaagcctttg aatttgaaat cgcaccagaa caagtccgag
     1141 attttctaaa aaaaagcctg caggaagacc atcgctcatc agcacaagga agctggatag
     1201 aaggaattct cctcaactgc ctcaaaccat ttttaggacg attagtgata tgagtaacac
     1261 ctaccgtgta aatttcatgg taggtgttat tgtaccccaa aataatataa aacaaagtat
     1321 gaaagcgcat ctcaatttta agactttttt gtaaattaag agtataatta aggtatactg
     1381 ccttttctat agataataga taatacacta atataataaa ggtctttgta acatatataa
     1441 attttatttc aaggaggaat aatggaaaag tattttggtg aaaaacaaga gcgtttttca
     1501 tttagaaaat tatcagtagg acttgtatct gcaacgattt caagtttatt ttttatgtct
     1561 gtattagcta gttcatctgt ggatgctcaa gaaactgcgg gagttcacta taaatatgtg
     1621 gcagattcag agctatcatc agaagaaaag aagcagcttg tctatgatat tccgacatac
     1681 gtggagaatg atgatgaaac ttattatctt gtttataagt taaattctca aaatcaactg
     1741 gcggaattac caaatactgg aagcaagaat gagaggcaag ccctagttgc tggtgctagc
     1801 ttagctgctc tgggaatttt aatttttgct gtttccaaga aaaaggttaa gaataaaacg
     1861 gtattacatt tagtattggt tgcgggaata ggaaatggtg tcttagtttc agtccatgct
     1921 ttagaaaatc atcttttgct aaattacaat acggactatg aattgacctc tggagaaaaa
     1981 ttacctcttc ctaaagagat ttcaggttac acttatattg gatatatcaa agagggaaaa
     2041 acgacttctg attttgaagt aagtaatcaa gaaaaatcag cagccactcc tacaaaacaa
     2101 caaaaggtgg attataatgt tacaccaaat tttgtagacc atccatcaac agtacaagct
     2161 attcaggaac aaacacctgt ttcttcaact aagccgacag aagttcaagt agttgaaaaa
     2221 cctttctcta ctgaattaat caatccaaga aaagaagaga aacaatcttc agattctcaa
     2281 gaacaattag ccgaacataa gaatctagaa acgaagaaag aggagaagat ttctccaaaa
     2341 gaaaagactg gggtaaatac attaaatcca caggatgaag ttttatcagg tcaattgaac
     2401 aaacctgaac tcttatatcg tgaggaaact atagagacaa aaatagattt tcaagaagaa
     2461 attcaagaaa atcctgattt agctgaagga actgtaagag taaaacaaga aggtaaatta
     2521 ggtaagaaag ttgaaatcgt cagaatattc tctgtaaaca aggaagaagt ttcgcgagaa
     2581 attgtttcga cttcaacgac tgcgcctatt ccaagaatag tcgaaaaagg tactaaaaaa
     2641 actcaagtta taaaggaaca acctgagact ggtgtagaac ataaggacgt acagtctgga
     2701 gctattgttg aacccgcaat tcagcctgag ttgccagaag ctgtagtaag tgataaaggc
     2761 gtaccagaag ttcaaccagc gttaccggaa gcagtcgtaa ccgacaaagg tgagcccgct
     2821 gt
//
LOCUS       DBERCA010000095         2798 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.095, whole genome shotgun sequence.
ACCESSION   DBERCA010000095 DBERCA010000000
VERSION     DBERCA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.095"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            323..1669
                     /gene="gdhA"
                     /locus_tag="ACL719_001926"
     CDS             323..1669
                     /gene="gdhA"
                     /locus_tag="ACL719_001926"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGS0699819.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2335..>2798)
                     /locus_tag="ACL719_001927"
     CDS             complement(2335..>2798)
                     /locus_tag="ACL719_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699820.1"
                     /translation="QDSFLLNRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMK
                     FNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQG
                     CTQIIIAHRLSTIKDADIIFVMKGGKIVESGNHKYLMTLGGEYYSLYIKRK"
BASE COUNT          882 a          528 c          481 g          907 t
ORIGIN      
        1 tttttgattt tagtctggta taaatattgc tttcctccaa aatggtcata attttactag
       61 caaatctaac atatcacgga taaattaaca agtgatttct gaattactaa acattttctt
      121 ttcttatagc ataccatact ttaagatttt gtctttgaga aagatatttc caagaaaaac
      181 gttcgttttt ttatctattt tcaaaaaaac caaaaactga attgaaagaa tttttagaaa
      241 atttctgttt tttctttaca gtgaaaaaaa attctgctat aatgtcttat gtaataaaat
      301 atgacaataa aaggagaacg atatgacatc tgctaaagaa tatatccaaa gcgtgtttga
      361 aactgtgaaa gctcgtaacg ggcacgaggc tgaattcctc caagctgttg aagaattttt
      421 caacactttg gaacctgtat ttgaaaaaca ccctgagtat atcgaagaaa atatcttggc
      481 acgtattact gagcctgagc gcgtggtttc tttccgtgtt ccttgggttg accgtgatgg
      541 aaaaattcaa gtaaaccgtg gttaccgtgt tcaattcaac tcagctgttg gaccatacaa
      601 aggtggactt cgtttccacc caactgtaaa ccaagggatc ttgaaattcc tcggatttga
      661 acaaatcttt aaaaatgtct tgactggact tcctatcggt ggaggtaaag gtggatcaga
      721 cttcgatcct aaaggtaaaa cagatgctga agtgatgcgc ttctgccaaa gcttcatgac
      781 tgaattgcaa aaacacatcg gaccatcact tgacgtacct gctggtgata tcggtgttgg
      841 tggacgtgaa attggttacc tttacggtca atacaaacgt cttaaccaat ttgatgctgg
      901 tgtcttgact ggtaaacctc ttggatttgg tggtagcttg attcgtccag aagcaactgg
      961 ttacggtttg gtttactata ctgaagaaat gctcaaagct aacggtaaca gctttgctgg
     1021 taagaaagtc gttatttcag gttctggtaa cgttgctcaa tatgctcttc aaaaagcaac
     1081 tgaactcggt gcaactgtta tttctgtttc tgactcaaat ggttatgtca tcgatgaaaa
     1141 tggtatcgac ttcgatcttt tggttgatgt taaagaaaaa cgtcgtgctc gtttgactga
     1201 gtatgcagct gaaaaagcaa ctgcaaccta ccacgaaggt actgtatgga cttacgctgg
     1261 aaactatgac attgctcttc catgtgccac tcaaaacgaa atcaacggtg aagcagctaa
     1321 acgtttggtt gctcaaggcg ttatctgtgt atctgaaggt gccaacatgc cgagcgacct
     1381 tgatgccatc aaagtttaca aagaaaatgg tatcttctac ggacctgcaa aagctgccaa
     1441 cgctggtggt gtagccgttt cagctcttga aatgagccaa aacagtcttc gcctctcatg
     1501 gactcgtgaa gaagttgatg gccgtctcaa agacatcatg acaaacatct tcaacacagc
     1561 taaaacgact tcagaaacat acggtcttga taaagactac cttgcaggag ctaacattgc
     1621 tgcctttgaa aatgtagcaa acgctatgat tgcccaaggt attgtttaag atttatttgc
     1681 ttaatcctca atcactacga ttggggattt ttagttgagt tagacagttg ggaaattcta
     1741 attaaatgaa ataaaaactg aacaaattga ttgcggaatt caaaccaatt tctaacatac
     1801 ttctagaaat taatttgact tttctaatcg atttgttcat atcttatttc aatttactat
     1861 acttacttaa ccctttttat actcaatgaa aatcaaagag caaactagga agcgagccgc
     1921 aggctgtact tgagtacggc aaggcaaagc tgacgtggtt tgaagagatt ttcaaagagt
     1981 attaaacaaa aagaaaaacc gctactccta agaatagcga tttaatcatg tttttaagct
     2041 actactgtag ttgctctatt atttaagtgt tatgaataac ttcaatatct ttaaagcttt
     2101 ttgttagatt tataatttca actatagcca tctctactcc ttagtttaca gcaaaaaatt
     2161 atgcttaatt cttattgttt ttgtaaacaa tcagcgaacc caatactatt ccattcaaca
     2221 agcctaaaga aataccatat tcaactggaa aatctaaaaa aattgcaaac actattccaa
     2281 tcatcacccc tagtgaggct attgttaaaa ttacatattc attttcttta cacctcattt
     2341 cctttttata tataagctgt agtactctcc accaagagtc attaagtact tatgatttcc
     2401 tgactcaaca atcttaccac ctttcattac aaaaataata tccgcatcct taatcgttga
     2461 caatctatga gctataatta tttgagtaca accctgactt tgtatatact ttgttattct
     2521 ttcctcatta atagtgtcta atgcactagt tgcttcatct aaaattacaa tactaggatt
     2581 atttattaat gcacgtgcca gtgctatccg ttgcctttgc ccacctgaaa tatttgaccc
     2641 catctctgag atgatagtat taaatttcat cggcatagcc atgatttcat catagatttg
     2701 aactgcttta caaacttcct ctatcttttg tgaagtaact tcgtgcttta aagttatatt
     2761 atcaagaata cttctgttca ataaaaacga atcctgtg
//
LOCUS       DBERCA010000096         2764 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.096, whole genome shotgun sequence.
ACCESSION   DBERCA010000096 DBERCA010000000
VERSION     DBERCA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.096"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..354)
                     /locus_tag="ACL719_001928"
     CDS             complement(<1..354)
                     /locus_tag="ACL719_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699821.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFLNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKV"
     gene            complement(412..1125)
                     /locus_tag="ACL719_001929"
     CDS             complement(412..1125)
                     /locus_tag="ACL719_001929"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGS0699822.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIGRDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1584..1964)
                     /gene="dhaM"
                     /locus_tag="ACL719_001930"
     CDS             complement(1584..1964)
                     /gene="dhaM"
                     /locus_tag="ACL719_001930"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGS0699823.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(1964..2038)
                     /locus_tag="ACL719_001931"
                     /pseudo
     CDS             complement(1964..2038)
                     /locus_tag="ACL719_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
     gene            complement(2386..>2764)
                     /locus_tag="ACL719_001932"
     CDS             complement(2386..>2764)
                     /locus_tag="ACL719_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGS0699824.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          842 a          540 c          439 g          943 t
ORIGIN      
        1 aactttttgt tcgtaggcat attttgctac atgatctagg tatatatcaa aaaagtcatg
       61 aggaaaatct cccatacagc cattacgatt catattgagg ctatattttc gattagtaag
      121 ataattcaga aatatatttc ctattactct aagattatct acttgtgaat cccttctccc
      181 aatataatcc ccttctttga ctaatgatgg agatttccaa gtaaaaatta aatcgccaga
      241 gaaatttttc ttatcaaaaa acttaaaaag agattgataa atactatttg ctaattgaca
      301 agtgtcagat gtaaattcat aattaacagc ttcattaaaa tcaacttcag acatttcaat
      361 gtactcttca atagtaatat tagctttttt gtagtcatta gctcgtctcc tttaaaatgt
      421 gatcttttca agaatatttt tatctaaaca atccagcaag tcttggtaag aatagacttc
      481 gtaagtcggc tgggcttgtg tgtgattttc gaggtgatga ggattatacc agatagtgtc
      541 aatccccgca ttattgccac cttgaatgtc ggcggttaga gaatctccaa tcatcagcgt
      601 cttttcttta ctaaatccag caatttgctg gccaatcttt tcataaaaaa gagcatccgg
      661 cttttgagtt tgcaactgtt ctgagataaa gacttgattg aaataaggtg ctagaccaga
      721 ttgagccaaa cgtcctgtct gaatggcagt aatgccattt gtcgcagcat acaagttata
      781 atcacgccca atgaggctgt ccaagagatc atgagcgccc gatagtgttt gtccctgctg
      841 ggcgaggtaa aattggtaac gctgggcaag aaaactaccg tctttttctt gtccaaaatg
      901 agcaaataaa cgagaaaagc gcgtgttaac cagctcttgt ttactgattt tcttcagctc
      961 caagtctttc cagagagcct tgttcatagg aacgtaataa tctttataag cctgaatatc
     1021 cgcaactcct tcttctttta gaagttgggt caaagccaca tcctcagcag catcaaaatc
     1081 aagaagagtg tggtcgaggt cgaagagtag aaatttgtag aacaatttga ggttttcctt
     1141 tctgaaaatt cattaagaac attatatcat aaagcacctc atacaattaa ctaatttaat
     1201 cacttaaaaa aaattcgaac actttctata caactgacag ctcaaatctt tcagaataga
     1261 acaatactaa ctatcgaaca ccctgtcttc ataaatacat atgtaattct aggcctagaa
     1321 ttcctataaa ctaaatgctt tcatactctt ccaagtaatt gattgcctta aattttaatt
     1381 tttgaaggtt tctaaagcta gaatagcccc atcacaatca gttttgattg attcacaatt
     1441 tagaaacact atagtttcac tcctgttaaa ataaaaagga actgcataaa gcaatccctt
     1501 tctgattttg aaatcattta cttaacattt tatagttgag ataatcaata gcttatctat
     1561 aaaaagagtt atagtaaaat tccttattta ttgatttcaa gctccgctaa ctgtatttga
     1621 ataactgaca gttctgcacc agcctgaaga agagcagctg cattataggc accttctaca
     1681 attggaaccc tgttgatgat gatactttta tcactgaaat cagtcaccat ttttaagttc
     1741 attttagcag aacctaggtc aaaaaaggca agtaaagtat ctgctggatt ttcggaaaca
     1801 accctatcta cttgatcaaa actcgttcca attcctccgc cctcggttcc tcctacataa
     1861 gtaatcggaa catctttagc tactttacta atcagttcaa caacaccttc tgcaatgtgt
     1921 ttggaatgtg aaacgataac aagaccaata ccaatacttt ccatcaaacc actccagttt
     1981 ctaaaatagc agtaaagagt aatcctgatg agaatgatcc aggatcaata tgtccaagaa
     2041 accacatgct cctaagacaa gagctaacag actggccatc aataatagta ttgttctttt
     2101 tttcatcatt actccttaac tagtgtttaa ctgattaatt agccagtaaa tagtttatct
     2161 ttatttacac tatctgttaa gatatagtaa aatgaaataa gaacaggaca gtcaaatcga
     2221 tttctaacaa tgttttagaa gtagaggtat actattctag tttcaatcta ctatattttg
     2281 cacattttca tgaaaaaaat gagaactaga actcacattc tgctctcatt tttcgttttc
     2341 ccgttctcct atcctgtttt taggagttag aaaatgctgc tacctttact tactctcctt
     2401 taataaagcc aatagttttt cagcttctgc cataatagta ttgttgtcct gggtgccaaa
     2461 tagtaaatta ttttttaatc ctgtgagagt ctctttggca ttggacttga taattggatc
     2521 ctggattttt ccaagtaaat cttcagcctc tctcagtttt tcttcaacct tttcagtctc
     2581 gacctgaggt tcttctgatt cctctggtga ttcttctggt tcctctgttg gttttggaga
     2641 ctctggtttc tcgctttgcg gtttctcttc tcgaggggtt tcttcctcag gtttttctgt
     2701 ctgaggtttc tcctcgcttg gtttttccgt ttgattggta tcagcttgac catttttgtt
     2761 tctt
//
LOCUS       DBERCA010000097         2636 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.097, whole genome shotgun sequence.
ACCESSION   DBERCA010000097 DBERCA010000000
VERSION     DBERCA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.097"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            12..479
                     /locus_tag="ACL719_001933"
     CDS             12..479
                     /locus_tag="ACL719_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699825.1"
                     /translation="MIDKVVRNLLLTFFFCKMTKIINFLTTIIVKKKKICYNEFKLRN
                     RKQKGVIMWVLGFILFMIFFYSNNSKKIKKLEKKIKRLERKEKGNAEMSRLLQEMIGK
                     EPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            489..695
                     /locus_tag="ACL719_001934"
     CDS             489..695
                     /locus_tag="ACL719_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0699826.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            695..892
                     /locus_tag="ACL719_001935"
     CDS             695..892
                     /locus_tag="ACL719_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699827.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            895..1629
                     /locus_tag="ACL719_001936"
     CDS             895..1629
                     /locus_tag="ACL719_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGS0699828.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1641..2282
                     /locus_tag="ACL719_001937"
     CDS             1641..2282
                     /locus_tag="ACL719_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0699829.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2300..2530
                     /locus_tag="ACL719_001938"
     CDS             2300..2530
                     /locus_tag="ACL719_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699830.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          777 a          436 c          538 g          885 t
ORIGIN      
        1 gaagagtatt aatgatagat aaagtggtca ggaacctact cctgaccttt ttcttttgca
       61 aaatgacaaa aatcataaac tttttgacaa ctataattgt caaaaagaaa aagatatgtt
      121 acaatgaatt caagttaaga aataggaagc agaaaggagt tataatgtgg gtactaggat
      181 ttatactatt tatgattttc ttttattcta ataattctaa aaaaatcaag aaactagaga
      241 agaaaatcaa aagacttgag cgaaaagaga aaggaaacgc agaaatgtcg agattattac
      301 aagaaatgat tggaaaggaa ccaattataa cgggagtgta tattgggcca gataactggg
      361 aagttgtgga tgttgatgag gaatgggtaa agctacgacg tgtagataat acgggaaaag
      421 aaaaattcaa gttgcaacgt attgaggata tccaaaccgt tgaatttgac ggagagtagg
      481 tgtgtaacat gcaactaaaa aatcgtctaa aagagcttcg agctcgcgat ggtctcaatc
      541 aaaccgacct agccaaactg gcaggggttt ccagacagac cattagccta ctagaacggg
      601 atgagtacac cccatccatt atcattgccc tgaaaatatc tcaaattttc aacgaaacag
      661 tcgaatcggt atttcgtttg gaggaggacg agtgatgaac aagtataaag tgatttatta
      721 tgtagtggtc atagctttat tagtcagcgt atttctactg attgggatgg acctaagctg
      781 gtttaatccc tatcaaagcg accaatttgt ttgggtctac tttgctctca tcccagtaat
      841 tgaatggatt gaaaagaaat ccaaaaatct agcaagtgaa aaaggagaat gaatatgaaa
      901 aagaaacgta gattgttatt tttgatgtct attgtcttgg gggggttctt gggcatgttt
      961 gtagggatgt ttaaggcacg tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg
     1021 atgccatgga tatcagctat ttgtttactg ataggtttca ttagtatgtt tttgactttc
     1081 aatttcttaa agaaaagcag aaaatttcat tccttgtatc aagaggaaat ggatgacgat
     1141 ctgaatgaaa cctattatgt gcaaatgtat cggaatcttg agtttggaac cattgctttt
     1201 aatattacag gtgtagcgat tccattggct atttttattt cattaagtga ggtgattata
     1261 ttgcatacaa accctcaaac atttttcctt tctttcttac tctttgtggt attcttagtc
     1321 gctcaaaaat ctctttttaa aaccattgcg attgttcgtc agtttgattt ggaatttttc
     1381 gctacaccaa aggatgtctt gaactatata aattcttatg atgaagggga gcgtcaggct
     1441 aatttggaac agagttttcg aattttattc caattacacc aatatgtctt accagcctta
     1501 tatatttttc ttattatcat ttctttcttg acaggagaga ttcagttact agctttcttg
     1561 cttgtaggag ccatccatgt ttatatcaat gtgatgcagt tacctatggt aaaacgttat
     1621 ttcaaataaa ggagttatct atgaaactac ttaaaaacct tggctggttt cttctagccc
     1681 ttctatcctt tttctttatc tatggcttta ttcaggggct cgcgactgcg tcgcttgctt
     1741 taggcgcttc accctatgct gttactctac tctatgtggt cttggctgga gtatatgtgt
     1801 acggcattta caaatggtat cagaaagctc ctgtttatat tgagaagagc ggctttaacc
     1861 gatttatttg gcttcctgcc ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc
     1921 cagatgatcc ttcagtaaat caacaaatag cgacagactt gaccttgtct caaccacttt
     1981 tctcattctt tgctgtggtt atttttgctc ctttgacgga agagattgtt tttagaggga
     2041 tgttagcacg ctatctcttt cctaagcagg acaatagtaa acgaaccctg atttttcttc
     2101 tggtatctag tcttctattt gccttgattc attttccagg tgatgtgcaa caattttttg
     2161 tctattttag ccttggtttt agtttgggtt tggcttacat tagcagaaaa ggtctggtct
     2221 acagtatttc tcttcacgct ttgaataatt tagtcggctt tttgatgatt ctcatgctat
     2281 aatagagtca ggaggtcaca tgaaacgagt aattttatta gcagtgattc aggcagtcgt
     2341 tctatttttc atcattggag cgctagctta tgccttcaaa ggcgatttct tctataacta
     2401 tctagcagtt gtctttgctc ctattgcagg tgtactgcgt tttgggacgg cttacataac
     2461 ggaaattgtc ttgcctcgaa aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa
     2521 ttcaaaataa aagagtccgg tgaagttcat cggatttttg tattatactc aatgaaaatc
     2581 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgt
//
LOCUS       DBERCA010000098         2558 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.098, whole genome shotgun sequence.
ACCESSION   DBERCA010000098 DBERCA010000000
VERSION     DBERCA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.098"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            46..666
                     /locus_tag="ACL719_001939"
                     /pseudo
     CDS             46..666
                     /locus_tag="ACL719_001939"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            663..1853
                     /locus_tag="ACL719_001940"
     CDS             663..1853
                     /locus_tag="ACL719_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS0699831.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(2363..>2558)
                     /locus_tag="ACL719_001941"
     CDS             complement(2363..>2558)
                     /locus_tag="ACL719_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699832.1"
                     /translation="RKRMNVAETLLLNTELPIKEIAESVGYSSASKFSIYYKRYKGKL
                     PSEVRNLACKHHNFKCDYCD"
BASE COUNT         1022 a          347 c          386 g          803 t
ORIGIN      
        1 ttatttataa caagaggata gaaatgaccg aattagaaag aaaaaatcga aaaattagct
       61 aagaaatatt ctgataactt aaacatcaaa gttcaagaga gagttcgtga aatggcaaat
      121 gataataaga gccattattt gatatacaga gttttaggta tttcatttga agaaggagaa
      181 aatatcgatt tgtatcaaaa taaaggtcgt tttttataca aatatgctgg ttcattttta
      241 gaagaagctg cagtactatc ctttaacgaa aaatttggta cagaaaatac ttaaaaagtt
      301 aacattccta attctgaaag tacaaaacct aagacttttg aaattgattg tttagtcgga
      361 gaaaaacacg catacgaaat aaaatggtgg gatgcaacta cagatggaga ccatataact
      421 aaagaacaca ctagaataaa agttattcat aacaaaggat atataccaat tcggttaatg
      481 ttctactatc caaatagaac tcaagctata aaaattcagc aaactttaga aacattgtat
      541 aacggtattg gagggaaata ttatggagat tctgcctggg aacatttaag agcagtgacc
      601 ggtattgatt tacttagtat tctaacagat attgcaaata aaaaaacagg ggtaaaatca
      661 aaatgacagt attaaaagga gataacttag aaatattaaa aactattgaa tcctcaagta
      721 ttgatttaat ctatatggac cctcctttct ttacacagaa aacccaaaaa ttatctaata
      781 acaaaaatat tatgtattca ttcgaagata cgtggacttc gattgaggat tacaaagaat
      841 ttttgtctgt aagattagaa gaatgcaaaa gagtgctaaa aaatagtggc agtattttcg
      901 ttcattgtga taaaattgca aatcatcata ttagattaat tttagataat atctttggag
      961 cagatatgtt tcaaagcgaa attatatgga actataagcg gtggtctaat tcaaaaaagg
     1021 gattattgaa caatcatcaa aacatttact tttattcaaa gtcaaaagat tttaaattta
     1081 atacaatttt tacagagtat tcttctacta caaatatcga ccaaatacta gtggaacgaa
     1141 aacgagatgg aaactctaaa actatatata aagttgataa taatggtaac tatattctag
     1201 caaaagagaa aaatggagtt cccctttcag atgtttggaa tataccattt cttaatccaa
     1261 aagctaaaga aagagtaggt tatcctacac aaaaacctat tctgttatta gaacaaatta
     1321 taaagattgc tactgataaa aatgatatag ttttagaccc gttctgtgga agtggaacta
     1381 ctttagtagc ctccaagatt ttgaatagaa attatatggg gattgattta tctgaggaag
     1441 ctatcaatat aactcagcaa cgtctggaaa atgttataaa aacaagttca aatttattga
     1501 ataaaggaat cgaagcatat agaaccaaaa ctgaggaaga ggaaaacatt cttaaattat
     1561 tacaggcaaa aattgttcaa agaaataaag gaattgatgg ttttttacct aaacattttc
     1621 aaaaaaaacc gatacctata aaaattcaaa aaaataatga atgtctgaat gagagtatct
     1681 ctttattaca gaatgctata aactccaaaa aacttgattt tggagtagtt ataaaaactc
     1741 attcagataa tttattattt gattttgata cgattcctga aaatataata gtagttgacc
     1801 attttgaact tacaatcgaa aaatggttaa gtaaaagtca acagttacta tagtttgcaa
     1861 attatttaca gaaagaacaa aaaatgctct gaaacactga tttcaaagca ttttttgtta
     1921 gtttaaaatt actcccattc ttcttttcca aacgtacaat atatccaaaa ccattcaaaa
     1981 tactagattc tattttttat aatatcacta aatccaccta attataggac gttttcagat
     2041 ttttagtccc agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct
     2101 tctaagctct taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata
     2161 ttagggcaaa taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat
     2221 agcaaaggtg cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca
     2281 tttttcaaat caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa
     2341 acatggcttg taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc
     2401 aagctaaatt acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa
     2461 atttgctggc agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt
     2521 ttaaaagaag agtttcagct acattcattc tttttctt
//
LOCUS       DBERCA010000099         2532 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.099, whole genome shotgun sequence.
ACCESSION   DBERCA010000099 DBERCA010000000
VERSION     DBERCA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.099"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..116
                     /locus_tag="ACL719_001942"
     CDS             <1..116
                     /locus_tag="ACL719_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS0699833.1"
                     /translation="TPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK"
     gene            140..1063
                     /locus_tag="ACL719_001943"
     CDS             140..1063
                     /locus_tag="ACL719_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HGS0699834.1"
                     /translation="MKIDVINHLDSPGLAGMICRRINNRILVYGGSYFPNNDPLKSSK
                     VQSNKIRVYDEKFNLLYEQDGKISPDKGITIEVENEIYYILGSSIYRITIGESVEETC
                     IGNFDFQLESGYGCYFDGHLFFGFQESYLFNIETQELFKKSDFPVSGRGQGLSVKYQN
                     ELYYLGGANNEAYLDGYKYSLKKDKWEKLEFELPSSVLGASSIQINESGLLILGGFNE
                     IVYNKAVIDLATPGYREEYFSKGRDFFNWNRSMRILDLETGKVKIIANDERFALCGAG
                     FLKTDNGYYVVSGECSPGRRTSDILLLEEEM"
     gene            1145..>2532
                     /locus_tag="ACL719_001944"
     CDS             1145..>2532
                     /locus_tag="ACL719_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0699835.1"
                     /translation="MKKIVKLALTAAAVLIAVVGLVACSSGSKKSDFSESGKEKVFYL
                     SGFLQGTGDRIPYVWQNGDGLVPYLLYRTLLMADSKYEKSTPDLAKDWKKSDDEKTYE
                     FTLKDGLKWSDGEALTASDVEFSVKLALKASVINGIFPSTFTKIEGAKDFKDDKADKI
                     SGLTVDGNKVTFKLTAPVGDFEQMMTQFFILPKHKLESENPLELNNSSFWTAPVTSGM
                     YKVKEISAGNFLEYDKNENYENEKPKFDKVVFNYISDPVLALQDGKSYFYSTNKPQEI
                     SQLDAVSTLSKKPIDILFYRYFIANLAGVEGNGDSLIANPKVREAILYAINRDELAKS
                     VFSGIADVNNTGVPSSLEAHLKSANKFEYNPEKAKQLLKEAKYDNSRNLILAYYYKDQ
                     ASQDFMQAVSYQLNEVGIKNEVVQITSDATTGLFKTRKYDLAYKGLSSFGYETWYGEY
                     SSTNTNFKNITNG"
BASE COUNT          922 a          333 c          478 g          799 t
ORIGIN      
        1 atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggcatc gttgttggtg
       61 gcgctattac tagaccaaaa gagattacag aacgttttgt agcgggatta aaatagcatg
      121 ccagagtaag aggtgtaata tgaaaattga tgttataaat catttagact caccaggatt
      181 agctgggatg atttgtagac ggattaataa tagaatttta gtttatggag gtagttattt
      241 cccaaataac gatcctttaa aaagttcaaa agttcaatca aataaaatta gagtttatga
      301 tgagaaattt aatcttctat atgaacaaga tggaaaaatt tctccagata aaggaataac
      361 gattgaagtt gaaaatgaaa tctattacat tctaggctcg agcatttatc gtattacgat
      421 tggtgaaagt gtagaagaaa cctgtatcgg gaattttgat ttccaactag agagtggtta
      481 tggttgttat tttgatggcc acttattctt tggatttcag gaaagttatt tgttcaatat
      541 tgagactcaa gagcttttta aaaaatctga cttcccagtt tctggtagag gccaaggttt
      601 gtcagtcaaa tatcaaaatg aattgtatta tttaggtgga gcgaacaatg aagcttattt
      661 agatggatac aaatatagtt taaaaaaaga taagtgggaa aaacttgagt tcgaattacc
      721 aagctcagtt ctaggagcat caagtataca aataaatgaa agtggtttat taatacttgg
      781 tggctttaat gaaattgttt ataataaagc agttattgat ttagcaaccc ctggctatag
      841 ggaagaatat ttttctaaag ggagagattt ctttaattgg aatcgtagca tgcgaatact
      901 agatttagag acaggtaagg tcaagattat tgcaaacgat gagcgttttg ctctttgtgg
      961 ggcaggattt ttaaaaactg ataatggata ttacgttgtt agtggggaat gtagcccagg
     1021 aagacgtact tctgacatac ttttgttaga ggaggaaatg taacgaagag tttaaatttt
     1081 taattactgc aatacttact taatcacata aataattact taatacaaat aaaggagtat
     1141 taaaatgaaa aaaattgtta aattagcttt aacagctgca gcagttttaa tagctgtagt
     1201 aggattggtt gcttgtagtt cgggaagcaa aaaatctgat ttttcagagt ctggaaaaga
     1261 aaaagtattc tatttatcag gttttcttca aggaactgga gatcgtatcc cttacgtttg
     1321 gcaaaatggt gatggtttag ttccatattt gttataccgt acactgttaa tggcagattc
     1381 aaaatatgaa aaatcaactc cagatttagc taaagattgg aaaaaatctg atgatgaaaa
     1441 aacttatgaa tttacactta aagatggatt gaaatggtct gatggagaag ctttgacagc
     1501 ttccgatgtg gaattcagtg ttaaattagc attgaaagct tcagtaatta atggaatttt
     1561 cccttcaaca ttcacaaaaa ttgaaggagc taaagatttc aaagacgata aagcagataa
     1621 aatttctggt cttacagttg atggaaataa agtaacattt aaacttactg ctccagttgg
     1681 tgactttgag caaatgatga ctcaattctt tattcttcca aaacataaat tagagtctga
     1741 aaatcctctt gaattaaata atagctcatt ttggacagct cctgttacaa gcggtatgta
     1801 caaagtaaaa gaaattagtg caggtaattt cttagagtat gacaaaaatg aaaactatga
     1861 aaatgaaaaa ccaaaatttg ataaagtagt attcaactat atttcagatc cagttttagc
     1921 attgcaagat ggtaaatcat acttctattc aacaaacaaa cctcaagaaa ttagtcagtt
     1981 ggatgctgta tctactttaa gtaaaaaacc aattgatatc ctattctatc gctactttat
     2041 cgctaactta gcaggtgtcg aaggtaatgg agattccttg attgctaatc ctaaagttag
     2101 agaagctatt ctatatgcaa ttaatcgtga tgaattagct aaatctgtat ttagtggaat
     2161 tgcagatgtt aataatactg gtgttccttc aagcttagaa gctcatctga aatcagcaaa
     2221 taaatttgaa tataatccag aaaaagcaaa acaacttctt aaagaagcta aatacgataa
     2281 tagcagaaat ctaattttag cttattacta taaagatcaa gcctctcaag atttcatgca
     2341 agcagtaagt tatcaattga atgaggttgg aatcaagaac gaagttgtcc aaattacttc
     2401 tgatgcaaca acaggattgt tcaaaacacg taaatatgac ctagcttata aaggattatc
     2461 atcatttggt tatgaaactt ggtatggtga atatagttca acaaatacta actttaaaaa
     2521 tattacaaat gg
//
LOCUS       DBERCA010000100         2407 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.100, whole genome shotgun sequence.
ACCESSION   DBERCA010000100 DBERCA010000000
VERSION     DBERCA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.100"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            22..807
                     /locus_tag="ACL719_001945"
     CDS             22..807
                     /locus_tag="ACL719_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699836.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPFVSEFWNDFKNNH
                     DKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            797..1348
                     /locus_tag="ACL719_001946"
     CDS             797..1348
                     /locus_tag="ACL719_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699837.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            1407..1667
                     /locus_tag="ACL719_001947"
     CDS             1407..1667
                     /locus_tag="ACL719_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699838.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          776 a          377 c          460 g          794 t
ORIGIN      
        1 atgatagaat aaggaaaaaa catgaatgtc aaaaagataa tgtcaatttt tcaatccttt
       61 tatgttgatg tcagtattga ggaactgact ttgactttac caatcagttt tgtaaaaagg
      121 tttgagtata ctcaaatgac ttttcataag gaatcatttt tattgattaa agaaaagaga
      181 agggggagtt tgagttcatt tgttactcag gctcgcacta tgggtgaaaa agccaatatg
      241 gatgttgttt tggtgttttc gaagttatca gacagtgaaa aaaagcaatt acttcaagct
      301 agagttccgt ttgtagactt taagggaaac ctcttcttcc ctccattggg actagtactc
      361 aatgcgaatg atactgaaat ccctaaggaa ttaacaccta gcgaacaatt aacgtggatt
      421 gcctttttat tgccagatgg cgatataaag cagataaaat ctgtttctaa ccttctatat
      481 ggtggtgctt atgctttgtc gcattcaact tttttagctg aaacggatga aaatactagc
      541 tatgtcatat ggcagagaaa attcaatcag ttatccttgc cactttctca gcatgtttta
      601 aaaggaaaga tgctagagat atggaaatat cgtccttttg tatctgagtt ttggaatgat
      661 tttaaaaata atcatgataa acaatttgta gatccgattt ctctttattt gaccttaaaa
      721 gatgatgatg acccacgtat agaggaagag agtgaagcac tagaaaatat gatattacag
      781 tatctgggag aagatgatgc cagctaatac gaaagttatt tttcaagaaa tgtttgcgga
      841 ttttcagaac tattatgttc tgattggggg aactgctacc tctatcgtat tggattcgca
      901 aggatttaaa agtcgcacaa caaaagatta tgatatggtc atcatttatg aagtaaaaaa
      961 taaggaattt tatactacct tgaatcattt tttagaattg ggagagtatc aaggaagtca
     1021 gaaagatgag aaagcgcagc tttttcgatt tacaacaact aatcctgagt ttccttctat
     1081 gattgaacta tttagtatct taccagaata tccattaaag aaggacggtc gagaaattcc
     1141 cttacatttt gaccaagatg ccagtttatc agccttatta ttggatgaag attattataa
     1201 tatattggtg catgaaaaag aaaccattca ggggtattcg gtattgagta attgtggttt
     1261 atactcttcg aaaatctctt caaaccacgt cagcttccat ctacaacctc aaaacagtgt
     1321 tttgagcagc ctgcagctag cttcctagtt tgctctttga ttttcattga gtattaatta
     1381 tttttaaggc taaagcttgg ctggatatga gggaacgctc tgctacaggt gctcaaggtt
     1441 taagtaagtc cattaaaaag catttgaatg accttacccg tttgacagct tccttgctag
     1501 gagatgaaaa gttatcggct ataacatcaa gtagtgcggt aaaagcagac atacaccgct
     1561 ttgtgataga attagagcct gtgaagtcaa ctattcttca aaatgatgac atttcattgg
     1621 atcaaaatga aatttttgaa attctgaaaa attttctcga tggttaaaat aattgtagcg
     1681 agatggctat attgaattcg tctatatctg gaaactagaa aaaacttcaa tttcaggaga
     1741 aaatgaagtc aatcttccca caatcaaacg tatagtatca aggtttttca agacctgata
     1801 ttatgcgttt tttgcttttc aaaacttttt gcctagtctt tgtttttatc ctctagtcac
     1861 ttgatttgtt tcaggtggtt ttttagtata gtgaaatgaa acgagaacag gacaaattga
     1921 tcaggacagt caaatcgatt tctaacaatg ttttagaagc agagatgtac tattctagtt
     1981 tcaatctact atagttaaat ctgcggtcaa gtctactggt gaatctatga ttgtaatact
     2041 cttccaaaat ctcatcaacc acgtcagtct cgccttgcag tctgtatctt actgaccaag
     2101 ctagtgatgg acttagaata ggtgatttgg agcgtcctat tggctaggaa atgctgatca
     2161 tagtcctttg ctgaggctag ggtgtttcaa cattcaacac tcaactggtt gatctagttg
     2221 ataggaaggg aattactata aaatactcag gcttccatca tattttttga aacgattgtg
     2281 taatcaaaat gtaccaatat tgtagtatta gtacagaaga tgttgtgaat ggataaatat
     2341 atcataactg ctgtctcaaa aagatttcat atgtctgtgc atatataata gacttcctgc
     2401 gaaacca
//
LOCUS       DBERCA010000101         2384 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.101, whole genome shotgun sequence.
ACCESSION   DBERCA010000101 DBERCA010000000
VERSION     DBERCA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.101"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..180)
                     /locus_tag="ACL719_001948"
     CDS             complement(<1..180)
                     /locus_tag="ACL719_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGS0699839.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILA"
     gene            complement(232..1668)
                     /locus_tag="ACL719_001949"
     CDS             complement(232..1668)
                     /locus_tag="ACL719_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS0699840.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKKVILSNGESVE"
     gene            complement(1858..2196)
                     /locus_tag="ACL719_001950"
     CDS             complement(1858..2196)
                     /locus_tag="ACL719_001950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HGS0699841.1"
                     /translation="MTLTIHVYYKGDGDCAIKFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGIILLIDQWINQEALDEHHQSNTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          803 a          509 c          291 g          781 t
ORIGIN      
        1 tgctaaaatc aaaggcaagt ctcctatacg aataccatga gcaagccaca atgcaatacc
       61 aataacttgc ataacataca tacctagagc aatagatcct gtgtcctttg tcttaactac
      121 acgaaaaact tgtggtaaaa atgcaaatgt tgttaaaatt gctgcaatac ttccaatcat
      181 atgtctcctc aatatgctaa acaaactgag aataatctca gtttgtttat actattctac
      241 tgattcaccg ttagataaaa taactttctt ataccagcca aaagattttt tcggggaacg
      301 attataactt cccttcccat tgtcatcttt atctacataa ataaagccat aacgtttccg
      361 catttcaccg gtaccagctg aaaccaaatc aatacatccc catggagtat aacccattaa
      421 atcaacacca tcttcaacta cagccttttt catttcacga atatgggcac ctagatattc
      481 aattctataa tcatcatgta ccataccatc tgctgcaact tgatctatag ctccaaaacc
      541 attttcaaca ataaagagtg gtaagtgata gtggtctgta aaccaattta acgcataacg
      601 taaaccttct ggatcaattt gccactccca ttcagaagcc ttaacataat tatttttcac
      661 taaatcttct gtttcaagat aatcaaaata aggattattt tcacgatgag agtctatagc
      721 aaaggacata tagtaactga aaccaatgta atctacagtc ccaccaagta aatcttcttt
      781 atcctggtca gtaaaatcaa ctgaaatacc ttttcgttcc caatacttga aaatatgctc
      841 aggatattta cctaaaacat gcacatcagc aaaataataa cgcttctgca tagctttcat
      901 tgccattaag atatccttag gattgcaagt aactggataa attggacaca tcgcaatcat
      961 acaacctatt tgaaaatctg gattaatctc atgaccaatt tttacagctc gtgcagaagc
     1021 aactaattcg taatgtgctg cttgatacat aattgcttct ctattatcac cttcctcata
     1081 tacaatacct gagttagtaa atggtgcaaa atcttcctga taattcgctt gattattgat
     1141 ttcattgaaa gtcatccaat atttaacctt atctttgtaa cgtttaaata cgacttctgc
     1201 aaaacgagca aagaaatcaa tcaatttcct atttttccaa ccaccatatt cggtcactaa
     1261 gtgataaggc atttcaaaat gagatagagt gatgacaggt tcaataccat tctttaagca
     1321 ttcatcaaaa agattatcat aaaactgtaa tccttcttca ttcggctcta actcatcacc
     1381 ttttggaaag atacgtgtcc atgcaataga ggtacggaag cacttgaatc ccatttcagc
     1441 aaaaagtgct atgtcttctt tataacgatg ataaaaatct atcgcctcat gatttggata
     1501 atatttaccc tctaaaactc ccaaagtaat ttcacgagct actccatggc gaccagcagt
     1561 cataacatca gcaacactaa ttcctttgcc accttcttgc catccgcctt caagttgatg
     1621 agcagcaaca gcaccacccc ataaaaatcc atctttaaaa gtagtcatct tttttcctcc
     1681 tgactttgat attcttatta taaacctcaa accaaaagat gaaaacgtat tctttttcct
     1741 tattattaag gaaagaagta atttttaatg gaaatagaac aatatcttct tgtattctcg
     1801 taatgatatc tttacgattt tcaatacttt caaactacaa aaactctcac aataattcta
     1861 attccctgtg tctataaacg acttatcgct ttctggcatc ccagaatcat cttctatata
     1921 acgttcaact tgcatctgca agtgatattt ttttcttaaa tctaagattt tctgcattgt
     1981 atttgattga tgatgttcat ctaaagcttc ttgatttatc cactgatcaa taaggagaat
     2041 aattccctct ttttcaattg gtaaaaaata ttcgtatttc aaattacctt tttgacatct
     2101 aatttctgcg acaagcccag attcaagcat ttctcttgca aactttattg cacaatctcc
     2161 atcgccttta taatatacat gaatagtcaa tgtcatctca tatcctccaa aatcatcctt
     2221 caattttaaa aaaacaagtt tagatgagga tctaaacttg ttttttatga actaattatc
     2281 taacgtttcg ccattacttt caatcacttc tttataccaa taaaatgatt ttttcttata
     2341 gcgatttata gtcaattgaa acaagaacaa gacaaaagag cctc
//
LOCUS       DBERCA010000102         2138 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.102, whole genome shotgun sequence.
ACCESSION   DBERCA010000102 DBERCA010000000
VERSION     DBERCA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.102"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            3..176
                     /locus_tag="ACL719_001951"
     CDS             3..176
                     /locus_tag="ACL719_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGS0699842.1"
                     /translation="MRQALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYMERYIE
                     DLKLKCNIANYNI"
     gene            232..549
                     /locus_tag="ACL719_001952"
     CDS             232..549
                     /locus_tag="ACL719_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HGS0699843.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            859..>2138
                     /gene="mobQ"
                     /locus_tag="ACL719_001953"
     CDS             859..>2138
                     /gene="mobQ"
                     /locus_tag="ACL719_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGS0699844.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEK
                     KILYYEIYKAKKEVYEEYQKKNIFTKEAFYNKHK"
BASE COUNT         1029 a          252 c          349 g          508 t
ORIGIN      
        1 ttattaggca agctttaaaa aatactgaat ataaagatca aatatctagc acagtattgc
       61 cagatatcaa aattaaatta ccgatagaca gtagaggtac tccagattgg aattacatgg
      121 aaagatatat agaagactta aaattaaaat gcaatatagc aaattataat atttaaaaca
      181 agaacgatta gaaataaaaa tctagtcgtt ttttattttt agaaaggata catgatggca
      241 agtaaaagat taagtattga agaacagatt gaaaagaaag aagaaagcat taagcaatta
      301 caaaatcaaa aaagacagtt gaagaaaaaa cttaatgaac aagaaagaaa agcaagaaac
      361 aaaagactta tagaaaaagg tgcagtgttt gaaagtattt ttgaagaaag tattgattta
      421 acaaaagatg aattttataa gttaataaaa acgttaaatg atgaagaaat aagattaaac
      481 ataatggaaa ttttagaaga aagaatagat gataatgtag aaaaatcatc taaagatgaa
      541 ataacataat tgtcccttgg ttacaagggc gcaattatac accctaaagg gtgcttgcgt
      601 cctgcggagt caaacccttg cagggagtaa caaccattcg ctttttaaaa gtcaagggta
      661 aataaactcg ctaaggctcg cccttgactt ttaaaatttg aaatgaaagt aacaattaag
      721 ccgacatact gaaacaacaa aaattaattc agtagtcggc ttttttaatt ctatcatttc
      781 aaaacgctca ttcacaaaga ggatataaaa aaactattaa gcctccacct aaaacaacaa
      841 agaaaggaag tgataaaaat ggcagacagt tttcattttt cagtaaacat aatatcaaga
      901 ggaaaaggca aaagtgcagt agcaagtgca gcatatataa gtggagaaaa aataaaaaat
      961 gaatgggacg gagtaaccca tgactataca agaaaagaaa aaatattagt aaaaaatata
     1021 atattgcctg atcatatacc aaaagaattt aatgataggt ctaccttatg gaataaagta
     1081 gaaatggcag aaaaaaattc taatgcacaa ctagcaagac aattcataat aggactacca
     1141 aaagagttat ctttaagtga aaataaaaac ctggttgaaa gatatataaa agaaaattta
     1201 acatcacaag gaatgatagt agattatgca attcatgatg agagtcaaga taaaaatgga
     1261 aatatccatt gtcatataat gaccataatg cgaccaataa acgaaaaagg agagttctta
     1321 gccaagtcaa aaaaagaata tatcctagat gaaaaaggag aaaaagtatt aaacaaaaat
     1381 ggaaaaccaa agacaagaaa agtagaacta acaacctgga acgatacagg caatgtagaa
     1441 aaatggagag aaaatttttc agatctttgt aacaaatatt tagaaagagc aggagcagaa
     1501 aaaagagtag accataggag ttttaaaaga caaaattcag attatctacc gacaatccat
     1561 ttaggatcag cagcaagtgc aatggaaaga aagggaatag aaactgacaa gggaaactat
     1621 aatagagaaa taagaaaata taatcaactt gtaaaaacaa taaaagaaga gataaaaaca
     1681 ttaaagggtt ggataggaaa tttattagat aacttatcta ctgcttatga aaaatttaag
     1741 gatatagaaa gagataaggt aatagacaac cctaaacttt ttaatctaac aaattatcta
     1801 ttaacttatt cagaaattca aaaagaaaaa agtaaatatc taaaaggata tgcaaaaact
     1861 aataaagaaa aatatgactt caaaaagcta acaagtgcat atagttattt aagaaaaaac
     1921 aacatagaaa ccataggtca gttacaaaca aaaatagaaa ctttaaagtc taatagttat
     1981 aggctaaata aaaaagcaaa gacaatccat aaagaaatgg aagatgtaga aaagaaaatt
     2041 ctatactatg aaatctacaa agccaaaaaa gaagtttatg aagaatatca aaagaaaaat
     2101 atattcacaa aagaagcatt ttataacaaa cataagaa
//
LOCUS       DBERCA010000103         2106 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.103, whole genome shotgun sequence.
ACCESSION   DBERCA010000103 DBERCA010000000
VERSION     DBERCA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.103"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            60..224
                     /locus_tag="ACL719_001954"
     CDS             60..224
                     /locus_tag="ACL719_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGS0699845.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            303..1031
                     /gene="yaaA"
                     /locus_tag="ACL719_001955"
     CDS             303..1031
                     /gene="yaaA"
                     /locus_tag="ACL719_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGS0699846.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1309..1857)
                     /locus_tag="ACL719_001956"
     CDS             complement(1309..1857)
                     /locus_tag="ACL719_001956"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS0699847.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
BASE COUNT          638 a          382 c          461 g          625 t
ORIGIN      
        1 gtttatttaa taaatatatg ttataataag gtaaattaag cctaatggag gacaaagaaa
       61 tgggttttta tttgatggtt gcttcaatgc tactcggact gctcgctttg aagataggtt
      121 tttctcagtt caaggagaaa aaagataaat tcttatctat cttaacaagt ttagctggca
      181 cagcccttgt tctcgtggct gtctggttag gatggcccaa ataaatagaa aaacctcggt
      241 caaaccgagg tttttcaaat gaatttcttg gttgtggcta aaaaatatgc tacactatca
      301 atatgaaaat tttaatccca acagcaaaag aaatgaacac agacttgcca agtatcgagg
      361 caattccttt aaaaccagaa agtcagaccg tgcttgatgc cttggctctc tattctgcca
      421 gtcaattgga gagtttctac aaggtatcgg ctgagaaagc ggcggaagaa tttcaaaata
      481 tccaagcttt gaaaaggcaa actgctcaac actatccagc cttgaaactt tttgatgggc
      541 ttatgtaccg caacattaag agagataagc tgaccgaggc ggaacaagat tatcttgaaa
      601 atcatgtctt cattacctcg gctttgtacg gtgttgttcc agccttgtca cccatggctc
      661 ctcaccgttt ggattttttg atgaaattaa aagtcgctgg taagactttg aagagccatt
      721 ggaaggcagt ctatgatgaa gctctgaaga aggaagaagt gattttctct ctcttgtcat
      781 cagagtttga gactgtattt tctaaggaaa tcagagcaaa gatggtgacc ttcaaattca
      841 tggaggatag aggcggtcag ctgaagattc actcaactat ctccaagaaa gcgcgcgggg
      901 cctttctaac agctttaata gaaaatcaag tacaaactgt gggggaagca cgtcgcttga
      961 actttgctgg atttgtttac cgagaagatt tgtcacaacc acagggattg gtttttgtaa
     1021 aggaagtata gaaatcagat attttgtatt tggcaatatg tatcatcagc tgttttagtt
     1081 tataaatgag aaaaaaagcg aacaagctag tcttctgagt gtcagaaaag tagtcttgtt
     1141 cgcttttatt atgttatact cttcgaaaat ctcttcaagc cacgtcagcg tcgccttacc
     1201 gtatatatgt cactgacttc gtcagttcta tctacaacct caaagcagtg ctttgagcag
     1261 cttgcggcta gcttcctagt ttgctttttg atttttattg agtgttagtt acttgatagc
     1321 ttcgaccaag tcttgagctt gtttttcaag tgagtttagg actgtttctt caagaaccaa
     1381 ttttccgtct gcccaggcag agtcattaac acgtgcagca gtgaaatcac caacgccttg
     1441 tgtacggata aatggcaaga ggtctttgta gatagcgaaa agttgatcgt gccctgcatt
     1501 ggctacagat gatactgtga caaacttgtc ttgaagggca gaaacgccac gtgtatcaga
     1561 caagtcaagg gcacgagata gccagtcaag caagtttttc actgtaccag ggatagagaa
     1621 gttgtagact ggagagaaaa tccagatagc atccgcaacg agaactgctt cacgagcagc
     1681 agctacagct ggatgtgttg gaacttccaa atcttggctg aagagaggaa gggctgaata
     1741 atcaaggtag ctaacttccg ctttaccagc aagagctttc tcagcttcga gcgccatttg
     1801 gtggttgaaa gaaccttgac gtagtgatcc gacgataaat aatacttttt tagacatgat
     1861 gtgtctcctt tttcttaaaa tttcgagaac tctctcgttg ttataaaata tattacaaca
     1921 aaacaaaaga acttgcaaga aatttgctca gagtttattt gaaaattgta aaatttcgga
     1981 aactctagtc atttgtgact gaaaaagtaa gaactgtttc actttcaaaa tcggttttgt
     2041 ttgaagggag aaatagaatt ggttgctcaa agcacagctt tgaggttgca gataaagctg
     2101 acgtgg
//
LOCUS       DBERCA010000104         1987 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.104, whole genome shotgun sequence.
ACCESSION   DBERCA010000104 DBERCA010000000
VERSION     DBERCA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.104"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            445..1920
                     /locus_tag="ACL719_001957"
     CDS             445..1920
                     /locus_tag="ACL719_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGS0699848.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINTIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1892..1987)
                     /locus_tag="ACL719_001958"
                     /pseudo
     CDS             complement(1892..1987)
                     /locus_tag="ACL719_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          595 a          370 c          451 g          571 t
ORIGIN      
        1 ttttttatca aaaaatactt tacaagttct taaaaacatg atatagtaat aaagcttaga
       61 aaatgagatg atgttttcta gcaaatataa acccgagtaa aaaatgccta cggacaggca
      121 gggttgaatg ccgaagcgtg gttgaaaagc cacattattg atagggttaa aagcctactt
      181 ttataagttg atgttaggac acttgttcta attcataaat ttttagtgtg gtgaaagcac
      241 acgtcatctt gtgaaacgat caataaagta cgtaatattt gctactagag agttaggaaa
      301 catcgggaac agacatactc aacagaaacc aaaataaaca cgtcagaaga ttgcagagca
      361 ggtgaaaacc tgctcttttt tcatgagtca acctttagtt ccttagtttt cataaggtcc
      421 taaaaatatt gaaaggagta tgttttgaaa gagttagatc aaaaccaagc cccaatttat
      481 gaggccttgg tgaagttacg caagaaaagg attgttccct ttgatgttcc aggtcacaag
      541 cgtggacggg gaaatccaga acttgtcgaa ctcttaggag aaaaatgtgt aggcattgat
      601 gtcaattcga tgaaaccctt ggataattta ggccatccta tttcgattat tcgtgatgca
      661 gaggagctgg ctgcagatgc ttttggagct agccatgcct ttctaatgat tggtggaaca
      721 acttcatcgg tgcagactat gattctggca acctgcaagg caggagataa gattattctg
      781 ccacgaaatg tccataaatc tgctatcaat gcgttggttc tatgtggtgc cattcccatc
      841 tatatcgaga tgagtgtaga tcctaagatt ggtatcgctt taggtcttga aaatgaccga
      901 gtagcacagg ccataaagga ccatccagat gctaaggcta tcctaatcaa caatcctact
      961 tactacggca tctgttcaga cctaaagggg ttgacagaaa tggctcatga agctggcatg
     1021 atggttttag tagatgaagc ccatggagcg catttgcatt tcactggtaa acttccaatt
     1081 tctgctatgg atgcaggagc tgatatggca gcagtttcca tgcataagtc tggtgggagt
     1141 ttgacacaaa gttcgcttct tttaatcggg gagcagatga atcccgagta cgttcgtcag
     1201 attataaacc tgacccagtc tacatctgcc tcttacttat tgatggctag tttggatatt
     1261 tcacgtcgca acttggccct tcgtggtaaa gagtcgtttg aggaagtcat tgagctatcc
     1321 gagtacgctc gtcatgaaat caataccatt ggcggttact atgcttactc aaaagagtta
     1381 atagatggtg tgtcggtctg tgattttgat gtaaccaagt tgtcagttta cactcaggga
     1441 ataggcttaa caggtatcga ggtttatgac ctcttgcgag acgaatacga cattcagatc
     1501 gagtttggtg atatcggcaa tatcttggcc tatatttcca tcggcgaccg catccaagac
     1561 atcgagcgct tggttggtgc tctggctgat attaagagac tctattcaag agatggaaaa
     1621 gatttgatag caggagaata tattcagccc gagttagtgc tgtctccgca agaagccttc
     1681 tattcagaga gaaaaagttt gaccttggac gaatctgttg gacaggtctg tggggaattt
     1741 gtcatgtgtt accctccagg aatccctatc ttggctcctg gcgaacgcat cacacgagaa
     1801 attgtcgact atatccaatt tgccaaggaa cgtggttgct ccctccaagg gacggaagat
     1861 ccagaggtca atcacatcaa cgtcattaag agaaagacaa actataagaa aagtcaatag
     1921 ttttatctaa actatttctt atttcaattt gatgatttgg cgatgatttt agagcatggc
     1981 aaaaagc
//
LOCUS       DBERCA010000105         1970 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.105, whole genome shotgun sequence.
ACCESSION   DBERCA010000105 DBERCA010000000
VERSION     DBERCA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.105"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1970
                     /locus_tag="ACL719_001959"
     CDS             <1..>1970
                     /locus_tag="ACL719_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HGS0699849.1"
                     /translation="ALPEAVVSDKGEPEQVAPLPEYTRPQAGAVVEPAIQPELPEAVV
                     TDKGEPAVQPELPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIV
                     EPEQVAPLPEYTGVQAGSIVEPEKVEAPKEYTGKIEQPSAEDTKPENEASSTNGESER
                     PKDKIKEEKQVDKKLELRNVSNVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENF
                     KDIMLPVTKIESTTKNNKEVYKIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYT
                     SFKNLVDAMNSDPNGTFHLGATMDAREVELPDDQESYVKNEFYGKLIGENNGKYYAIY
                     NLKKPLFKTLNTATIQNLSIKEANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERG
                     IGGLVSKVDNSRISNSSFTGRITNTYDTTVGYEIGGLVGKLSGSLASIEKSIASIDIA
                     SNAKSGDQIVGGIAGVVEKSATIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSK
                     SGKLSYVLSDVNVTNGNAISGDNFDNMKEDHAYSSKGNKVVNVVQVDDELVTKDSDVQ
                     RGTVLDADKVKEKKAELVSKHSTKVEDFDFTSRYTTIYNEVTGYQKSREQVYKNIEKL
                     LPFYNRETIVKYGNLVDESSELFTKELLSVVP"
BASE COUNT          757 a          287 c          408 g          518 t
ORIGIN      
        1 gcgttacccg aagcggttgt aagtgataaa ggtgaaccag agcaggtagc cccattgcca
       61 gaatatacaa gaccacaagc aggtgcagta gttgaacctg caatccaacc agagttacca
      121 gaagctgttg taactgacaa aggagaacca gcagtccaac cagagctacc ggaagcggtt
      181 gtaactgaca aaggtgagac tgaggttcaa ccagagtcgc cagatactgt ggtaagtgat
      241 aaaggtgaac ctaaacaggt agcaccgctt ccagaatata caggaccaca agccagcgct
      301 attgttgaac cagagcaagt agcaccacta ccagaataca caggggtaca agccggatcg
      361 atagtcgagc cagagaaagt agaagcaccg aaagaatata ctggtaaaat cgaacaacct
      421 agtgcagaag atactaaacc tgaaaatgag gcaagctcta caaatgggga atcggaacgt
      481 cctaaagata aaattaaaga agaaaaacaa gtcgacaaga aactagaact tagaaatgtt
      541 tcaaacgtgg aattatatac ggtagaaaat aataaatatc gacatattac ggctgtagat
      601 ggtgctttag atagttcatt gaagtatttc atgaaggtaa aatcagaaaa ttttaaagat
      661 ataatgttgc ctgttactaa aattgaaagt acaacaaaaa acaataaaga agtatacaaa
      721 attgtggctc atgcagaaaa tctaattcaa cacgaaaaca atgttatttc gaatgactat
      781 acatattatt taccaaaaac tcagcaaagt gaaactggcg tctatacatc atttaaaaat
      841 ttagttgatg caatgaatag tgaccctaat ggaacattcc atttaggtgc aactatggat
      901 gctcgtgaag ttgaacttcc ggatgatcaa gaaagttatg taaaaaatga attttacgga
      961 aaattaatag gtgaaaataa tggtaagtat tatgctattt ataacttgaa aaaaccttta
     1021 tttaaaactc taaacaccgc tactattcag aatctttcaa taaaagaagc gaatgtttct
     1081 tctaaagaag atgctgctac aatatcgaaa gaagcaaaat acaatactct tatagataat
     1141 gttcattctg atggtattat agctggtgaa cgtggaattg gtggattggt ttcaaaagta
     1201 gataactcga gaatttctaa tagtagtttt acaggcagaa taactaacac atatgataca
     1261 actgtagggt atgaaattgg tggtttagta ggtaaacttt ctggttcgct tgcttctata
     1321 gagaaatcaa tagcatcgat tgacatagcc tctaatgcaa aatcaggaga tcaaattgtt
     1381 ggtggtatag ccggagttgt tgagaaaagt gcgacaatta agtatagcta tgttgaagga
     1441 aatgttaaca atgtgcgaca ctttggtaaa gttgggggag ttgcaggtaa tctttgggat
     1501 agagattcac aagatgtaag taaatcaggt aaactatcat atgttttaag tgatgttaat
     1561 gtaacaaatg gtaatgctat atcgggtgat aattttgata atatgaaaga agatcatgct
     1621 tatagtagta aaggcaataa agtagtaaat gtagtccaag tagatgatga acttgtgact
     1681 aaagattcag atgtacaaag aggtacggta ttagatgctg ataaagtaaa agagaaaaag
     1741 gcagaattgg tttccaaaca cagcactaaa gtagaagatt ttgatttcac ttcaagatac
     1801 actactattt acaatgaagt aacaggttat caaaagtcga gagaacaagt atataaaaat
     1861 attgaaaaac tattaccgtt ctataatcga gagacaattg ttaaatatgg taatttagta
     1921 gatgaaagca gcgagctatt tactaaagaa cttctatcag ttgttcctat
//
LOCUS       DBERCA010000106         1677 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.106, whole genome shotgun sequence.
ACCESSION   DBERCA010000106 DBERCA010000000
VERSION     DBERCA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.106"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            210..914
                     /locus_tag="ACL719_001960"
     CDS             210..914
                     /locus_tag="ACL719_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGS0699850.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            895..>1677
                     /locus_tag="ACL719_001961"
     CDS             895..>1677
                     /locus_tag="ACL719_001961"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HGS0699851.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLS"
BASE COUNT          595 a          206 c          283 g          593 t
ORIGIN      
        1 agaaaggttc tattctaatt gctaaacggc ttgaggtata tgctactgtt atagaaatga
       61 ttttaaacta aaatcaaaat tactccaaaa atagtattga ttttggttta atacaaaaca
      121 tatgtaataa tgtgagtcaa caaaagtctg tttttgtacc atactatctt gaaaaatcta
      181 ctaaattcaa attgagagga tgaattaaaa tgaataaaat atttatatat gcaggagtgc
      241 gaaatcataa ctctaagact ttagagtata ctaagagact ttcaagtatc atttctagta
      301 gaaataatgt tgatatatct ttcaggacac ctttcaattc agagctagaa atatctaatt
      361 ctgattcaga agagttgttt aagaaaggaa ttgataggca aagtaatgct gatgatggag
      421 gtgttataaa aaaagagtta ttagagtctg atattataat tatctcttct cctgtatatt
      481 tacaaaacgt atctgtggac acaaaaaact ttattgaaag gattgggggg tggagccatc
      541 tttttcgatt agctggaaag tttgttgtaa cattagatgt tgctgagagt aacggctctg
      601 ataatgtgtc agaatatctt agagatatat tttcatatat gggaggacag atattacacc
      661 aagtttcaat taccaattca ctcaaagata ttgcagaggc tcagttgatg gaggcaactt
      721 ataaaattga agatgtactt gaagggaaaa ttaagtacaa aacaacggat tatcaagaaa
      781 gagcttacca gacattaaaa ttaattttag aaaattatga ttccgaacat tttgagaaga
      841 tgtattggga gaaaaaacgc ctttttgaag ctaatagttt agaggaatgg tattatgttg
      901 aaaatattaa ataactctgt catatattta tgtttgattc cgttattgtt cctattattg
      961 ataatttttc caaatgattc attaatctat tattttcgat tgattttaat ttcgttaata
     1021 tcaattacat tacatgaact aggtcatttt ttagtgggaa gatgtttatc ttataaacta
     1081 gagatgcttg ctactccctt tttcttctat ttcagaaaaa aaatatattt caaatttcca
     1141 gtgttattag cattcggtta ttgtcaaatg agtaatagaa acattactaa tgaaaaaaat
     1201 agtgatagga atttggtgtt ctacttcttt gggggaggtg gagctaacct aattgtcgca
     1261 atattagcac ttttgggatt tgtgccgtac gcatcagaat tttttatttt gaatataatt
     1321 ctttttctag ttacggtatg tttaccaatt gatggaacag atggcaatgc tattagagag
     1381 atagttttgt attcaaaaga ttcaaaaaca tatcagagat tctttgcgaa cagtctctat
     1441 aataatcctt atataacaat cgacgatttt gcaaaattaa cctctaaaga aaaatctttt
     1501 ttttcaaaat ttgaaaaatg tttgataaac ctttactttg aagtgaaagg tgaagggtca
     1561 gcatttacaa ttgctaatca tgaagttttt gattcaaata ttcaggaaaa tattatccat
     1621 gagtactata aattactaca taaatcttct gattggatta ttccacagaa cttatct
//
LOCUS       DBERCA010000107         1651 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.107, whole genome shotgun sequence.
ACCESSION   DBERCA010000107 DBERCA010000000
VERSION     DBERCA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.107"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            61..517
                     /locus_tag="ACL719_001962"
                     /pseudo
     CDS             61..517
                     /locus_tag="ACL719_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            682..981
                     /locus_tag="ACL719_001963"
     CDS             682..981
                     /locus_tag="ACL719_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGS0699852.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1242..1565
                     /gene="tnpB"
                     /locus_tag="ACL719_001964"
     CDS             1242..1565
                     /gene="tnpB"
                     /locus_tag="ACL719_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0699853.1"
                     /translation="MMILLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
BASE COUNT          555 a          326 c          292 g          478 t
ORIGIN      
        1 attgtgtact atttttggtt catttcacta taataataac gatactagat aatgaggaaa
       61 atgcaatggc aaacccaact ttcggagaaa aaaagcgaat acagactata ttgctcgcta
      121 tggcgtatat gcagttatcc ctaatcctga acaaaaacaa attgttcttg ttcaagaacc
      181 aaatggtgct tggttcctac catgtggaga aattgaagca ggtgaaaatc atcaggaagc
      241 cctaaagcgt gagttgattg aagagcttgg tttcacagca gaaattggta cctattacgg
      301 acaagctgac gaatatttct attctcgtca tcgtgatacc tactactaca atcctgccta
      361 cctctatgaa gcaacttctt tcaaagaagt acaaaagcca ctagaaaact ttaatcatat
      421 tgcctggttc cctattgacg aggctatcca aaaccttaaa cgtggtagcc ataaatgggc
      481 cattgaatct tggaaaaaac agcataagat tggctaatac tcttcgaaaa taaaattcaa
      541 accacgtcag cgtcgcctta ccgtactcaa gtatagcctg cggctatctt cctagtttgc
      601 tctttgattt tcattgagta taaatcaagc gactttattc aaaaagtgct ataatagaat
      661 tagaaaatcg gaggaaatat tatgaagctt atcaatacga caaactcaca ctcgcaactg
      721 gtaaaaagtc agctagaaag taccgatgca acccttgttg aggtttattc tgcagggaat
      781 acagatgtta tttttaccca agctccgctt cactatgaaa tcctcatctc aaacaaacac
      841 cgtgctattc gtgagcctga aatcgaaacc attcaagaat ttttcttgaa acgtaaaatt
      901 gataagacct ctgttgatga agccaatatc aagacactct actcagagaa attaattgga
      961 atttcaatcc caatcaagta agaaaattga gtaaaaactc gatttgagag aaagtccaat
     1021 aagtctttcc ataacaaaac gcatagtaac aagagtttct acacccacta ttatacgttt
     1081 ttatcatttt gaaatctgct gctaatcaat gaagaagcaa agatcaaact aggcagctag
     1141 ttacaagtta cgtaagcgcg tcataacaag gtatctatcc taattcccca acttccagta
     1201 tactagataa aaaataatta tcagaggaaa ggtattgtac tatgatgatt ctactaagtg
     1261 atttaggtca agttcacctt gtttgtggca agacagatat gaggcaggga atagactcat
     1321 tagcctatgt agttaaaacc cactttgaat tggatccttt ctccggtcaa gtctttctct
     1381 tttgtggtgg acgtaaatac cgctttaaag ccctctactg ggatggtcaa ggattttggc
     1441 tactatataa acgctttgag aacggcagac tgacttggcc cagtacagaa aaggatgtca
     1501 aagctctcac atctgaacaa gtagactggc ttatgaaggg cttttctatc actccccaaa
     1561 tatagtggat ttaaactaga atagtacacc tctacttcta aaacattgtt agaaatcaat
     1621 ttgactgtcc tgatcgattt gtactgttct t
//
LOCUS       DBERCA010000108         1484 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.108, whole genome shotgun sequence.
ACCESSION   DBERCA010000108 DBERCA010000000
VERSION     DBERCA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.108"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            77..850
                     /locus_tag="ACL719_001965"
     CDS             77..850
                     /locus_tag="ACL719_001965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0699854.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGIGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            863..1195
                     /locus_tag="ACL719_001966"
     CDS             863..1195
                     /locus_tag="ACL719_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699855.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKNS"
     gene            1287..1484
                     /locus_tag="ACL719_001967"
                     /pseudo
     CDS             1287..1484
                     /locus_tag="ACL719_001967"
                     /inference="COORDINATES: protein motif:HMM:NF045019.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
BASE COUNT          552 a          148 c          280 g          504 t
ORIGIN      
        1 gcatttatac aacttattta acactatctt tattcttgtg atataatgcc aaattataga
       61 gtggaggtca tttaaaatga ttgataaagt aattgaacaa tataaaagtc atgataatct
      121 tgatattcga gtagaattgc ataagaagta ttcaaaaaat aaattgggat ttaataattg
      181 gatattttct aactaccaaa tcactgatga agtaaaggtt ctagaattgg gatgtggtat
      241 aggagaattg tggaaaagta atagtgattc tatagataaa atgaagcagt tgattgttac
      301 agatttttct aaagatatgg taaaatcaac gaagtcagtg attggaaata gaaacaatgt
      361 caattatgag ataatggata ttcaaaagat ttcttttgag aatgaaactt ttgatattgt
      421 tattgctaat atgcttctac atcatgtgaa tgatattcct aaagctctct ctgaggtgaa
      481 tagggtctta aaaaccggag gaatttttta ctgtgctaca tttggtgaaa atggagttgt
      541 aaattatttg gcaagtttat ttaaagatga ggttaatcaa gatttagaaa atagaacgtt
      601 tactttacaa aatggtaaaa ggtacctgag taggtatttt aactctgtcg atacattact
      661 ctatgatgat gagttacagg taactagtat agatgaccta gttaaataca tccaatcgtt
      721 taaaggaatt tcagaaatag gttcactaga agaggaaata atacgtaaaa gattggaaag
      781 tgagtttaat aatggtatgt tgatcattcc taaagaatat ggtatgttta tcgctcgaaa
      841 agaaagttaa gggaatgaaa gtgtgaaaaa ttacggagaa gcttttcgat attttagaaa
      901 attaaacgga tattctttag agtatgctgc agctgattct atatcaaaat ctcaactttc
      961 tagatttgaa aggggagaaa atgaaatttc tctttcaaca ttttttgaat tattatcaaa
     1021 tatcaatgtt tcgatagaga atttttgtaa tcacttagaa cattataaaa gatcggagcg
     1081 tgatgatttt ttggttaatt tatctccaaa tttttatagt ttgaatatta aaggattgga
     1141 ggtaataaaa aataagcaac aaaaactttt tgagaagagt gggaaaaact cataaaataa
     1201 atacaattat ggttcaggga ttatgaaatc agctagattg taatcatgta gttagtagag
     1261 atgatctaaa tttagtatat gactatttat tccagaagga acgatgggag tcctatgaga
     1321 taacgctgat tggtaattta tatcatcttt ttgaaataga ttatatttac atggtcggaa
     1381 aagaaatact agagcgtaca cattattacg aaaaaatcgg caagaataga aatttagttg
     1441 tgtcagcttg tttaaatttt tggttctgtt gccttgaaaa ttca
//
LOCUS       DBERCA010000109         1404 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.109, whole genome shotgun sequence.
ACCESSION   DBERCA010000109 DBERCA010000000
VERSION     DBERCA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.109"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1404
                     /gene="pcpA"
                     /locus_tag="ACL719_001968"
     CDS             <1..>1404
                     /gene="pcpA"
                     /locus_tag="ACL719_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGS0699856.1"
                     /translation="KTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQ
                     NNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQ
                     AFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFD
                     FSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLP
                     KSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKT
                     PKGTKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAF
                     YGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHI
                     KNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVA
                     KPKKNSNQGVVGWVKDKG"
BASE COUNT          547 a          195 c          229 g          433 t
ORIGIN      
        1 aaaaactaca atattatcat taactacagc tgcagttatt ttagcagcat atgtccctaa
       61 tgaaccaatc ctagcagata ctcctagttc ggaagtaatc aaagagacta aagttggaag
      121 tattattcaa caaaataata tcaaatataa ggttctaact gtagaaggta acataggaac
      181 tgttcaagtg ggtaatggag ttactcctgt agagtttgaa gctggtcaag atggaaaacc
      241 attcacgatt cctacaaaaa tcacagtagg tgataaagta tttaccgtta ctgaagtagc
      301 tagtcaagct tttagttatt atccagatga aacaggtaga attgtctact atcctagctc
      361 tattactatc ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc
      421 taaaactatt atttttgaca aaggtagtca gctggagaaa attgaagata gagcttttga
      481 tttttctgaa ttagaagaga ttgaattgcc tgcatctcta gaatatattg gaacaagtgc
      541 attttctttt agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa aattagaatt
      601 aatatcacat gaggcttttg ctaatttatc aaatttagag aaactaacat taccaaaatc
      661 ggttaaaaca ttaggaagta atctatttag actcactact agcttaaaac atgttgatgt
      721 tgaagaagga aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac
      781 ccaattaatt tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggggac
      841 aaaagaactt gcatcatatt cgtttaataa aaattcttac ttgaaaaaac tcgaattgaa
      901 tgaaggttta gaaaaaattg gtacttttgc atttgcagat gcgattaaac ttgaagaaat
      961 tagcttacca aatagtttag aaactattga acgtttagcc ttttacggta atttagaatt
     1021 aaaagaactt atattaccag ataatgttaa aaattttggt aaacacgtta tgaacggttt
     1081 accaaaatta aaaagtttaa caattggtaa taatatcaac tcattgccgt ccttcttcct
     1141 aagtggcgtc ttagattcat taaaggaaat tcatattaag aataaaagta cagagttttc
     1201 tgtgaaaaaa gatacatttg caattcctga aactgttaag ttctatgtaa catcagaaca
     1261 tataaaagat gttcttaaat caaatttatc tactagtaat gatatcattg ttgaaaaagt
     1321 agataatata aaacaagaaa ctgatgtagc taaacctaaa aagaattcta atcagggagt
     1381 agttggttgg gttaaagaca aagg
//
LOCUS       DBERCA010000110         1168 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.110, whole genome shotgun sequence.
ACCESSION   DBERCA010000110 DBERCA010000000
VERSION     DBERCA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.110"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            11..148
                     /locus_tag="ACL719_001969"
     CDS             11..148
                     /locus_tag="ACL719_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS0699857.1"
                     /translation="MAAEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            942..1160
                     /locus_tag="ACL719_001970"
                     /pseudo
     CDS             942..1160
                     /locus_tag="ACL719_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          370 a          162 c          244 g          392 t
ORIGIN      
        1 ggagctagct atggcagcag aagtattaaa tcttcaatta gtatctgttc aagttgacga
       61 aacagatgaa gttgatggaa tgcgatttag tacattcagt acaaaccgtt gcggtaactg
      121 gagtgctttt agttgggaaa attgttaatt catttaaatt gtaaacttta gagattctct
      181 ggagttatgg atgtcgttcc aatatgtgca cgttggaatg ttagtgctta tatgggggga
      241 acagaatcct ctcttgattg aagacaagct agtcattagg ctggtttgtc tttttgtcaa
      301 ctgtagtggg ttgatataat agtattagtg agtgggataa aagtttcatt tagtttattc
      361 agtacaaatt taacgggtca agatttatat actagtggtg tttttggggc tgagagaagt
      421 atcttgattt tatgtgtggt ttttatactt acagttgttc tgctccaaag agcttgtaga
      481 gaagaattag ctcataaagg agattgatta ttttgatatc aaaaaaatgc acaggataac
      541 ctgatgcatt tttttagcga caatgcttgc tacttccttc tgtcgaattt agacaatttt
      601 aaataaaaga atgaaatatt ccctactgta tttttagacg aagaacgtta gttaataata
      661 ctcaaattta tttcaaactc tttgattagt atgaatttag ctctacagaa aaataaaaaa
      721 tgtgataaaa atttcaacta ctcttatcta taataaagct acctgttctt tgaaaattga
      781 atccactccg tcttgattag cgtgcctgtg taagtaggga ctgagagtat ttccctgtga
      841 agggcaactg gcacaagacg tgtgtgagaa ttttctaaca gacacggagt ttatcgtttc
      901 cagactcaaa cgatgcgaca aactagataa aggagttaat catgaaggta gtaaacttgt
      961 atgatttgaa acaaatggga aataaaggtg gttgtaccat ccaattgatt catcactttc
     1021 cttttggtat gggcttagga catctcaaaa aagactacat tgaatttaaa cgtgttggta
     1081 tctttgatgg gaaagcagta gaagttactc ttcgagaacc ttattcgaga gatctacttc
     1141 aggttgtcaa gtcaataaag taactgac
//
LOCUS       DBERCA010000111         1088 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.111, whole genome shotgun sequence.
ACCESSION   DBERCA010000111 DBERCA010000000
VERSION     DBERCA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.111"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            95..898
                     /locus_tag="ACL719_001971"
     CDS             95..898
                     /locus_tag="ACL719_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGS0699858.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQARGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNARTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1014..>1088)
                     /locus_tag="ACL719_001972"
     CDS             complement(1014..>1088)
                     /locus_tag="ACL719_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS0699859.1"
                     /translation="YNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT          332 a          186 c          212 g          358 t
ORIGIN      
        1 aacctattgt gaggactttt tatagtttaa gccagtttcc aaccaatttc gtgatttact
       61 ggtaatgaag aaaaacaatt gaaaggatat ttctatgcaa ccaacttaca acattgacaa
      121 tccaaacttg tcttatgaag ctaagcgtga tttatggcgg ataggttttg gtctgcagaa
      181 agttgacaat ctagtgccat cagcttatat ggaatctttg gctgagaaac aggctcgggg
      241 tgaactgact tatgagcagg tttatgagga tgcaacggct tatcaccata ctattgatgc
      301 aagtacggag gaggcagact tggtttctct acgcattgta gaactattgt ctcgaagagg
      361 ctttagcttc agtcctgcga ccttacttgc tattcataag gagttgtttc aagatatatt
      421 tgaaccctcg attccggtag gtcaatttcg tcagactaat atcacaaaga atgaacctgt
      481 tttgaatggt gaaagtgttg tgtactctga ttactccatg attcaaatga cattggatta
      541 tgattttaat caggaaaaac aagttgcata tgcgacacta acccaggcgg atatggttaa
      601 aaaaatccag cattttattt caggaatctg gcagattcat ccatttcgcg aaggaaacgc
      661 tcggacagta acggtctttt tgattcagta tcttcgtgag tttggttttg atattgataa
      721 tataccattt cagcaacatt ccaagtattt tcgtgatgcc ttggtattag ataatgcaaa
      781 gattttacag cgacgtcctg agtttttaac agcttttttt gaaaatctct tgctcggtgg
      841 tcaaaatgat ttgtcttcag aaaaaatgta tctagattta gacctcgatt tttcataatc
      901 caaatactga gtaaacattg aattttagga aaaaatgaag taaacattcc cacaataaaa
      961 cgcatagtat caagtgttaa aatgtactgc cccccaaaag ttagacaatt aatttatccg
     1021 aaggatttag ttctgtattg cacagggcta agtcctttta gttttacctt aattctctta
     1081 ttgttgta
//
LOCUS       DBERCA010000112         1085 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.112, whole genome shotgun sequence.
ACCESSION   DBERCA010000112 DBERCA010000000
VERSION     DBERCA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.112"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..135)
                     /locus_tag="ACL719_001973"
     CDS             complement(<1..135)
                     /locus_tag="ACL719_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGS0699860.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     A"
     gene            complement(147..713)
                     /gene="amaP"
                     /locus_tag="ACL719_001974"
     CDS             complement(147..713)
                     /gene="amaP"
                     /locus_tag="ACL719_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGS0699861.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(797..961)
                     /locus_tag="ACL719_001975"
     CDS             complement(797..961)
                     /locus_tag="ACL719_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS0699862.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          365 a          215 c          153 g          352 t
ORIGIN      
        1 agctgcaact cccagtgctc ctaaaatcaa tacaaatatt gttttgaaga agccaaagga
       61 gacaatcaaa caagccagaa atacgcctat gagaccagcg ataattggat atcgatattg
      121 tttaagccat tccatttttt acttccttac ttcacacgac taacagtctt tttgttttga
      181 ggttttggtt ggtaattttt tactgcaact tcaagtttta cttgacgctc aataccaaaa
      241 aactgcttca atccattagt tatttcattt tgaattattt ggcatctgtc agcgatgttg
      301 tctgaaggaa gaattttacc ttctacatga acgaaacatt tattttttcg taaatttaca
      361 tgaacagttg ggttcttgat caatctatga tcactcacca aacttctaac aaaaccttcg
      421 attgccgaat tctttaattt taatgtatcg tttttagttt caagttggat ttccaagtaa
      481 cgtttaggat aaaacatcac aactaaaatg gataataaaa ctaaagttga tagaaccact
      541 gtcccccaaa agacatatct agcaagatag aattcaacta cggagttctg tctccagctc
      601 agtagatgaa tacctagatc actaacttga tgataatcta tcaaaacagg aaggaaaatt
      661 gtcaagatta aaatacagaa aataatgaaa catattttct ttgcttttga catatacaat
      721 cctttcgatt taacatttaa actatttttc ccgtcattga tttaaaaata gtttgctaac
      781 tttactggcg aaaagtttac tttctacctg taaagaatga cactacagta atcacaatcg
      841 ctgcacctac aatagatggg agcaaagcca ttccagcgat tgatggaccc caactaccta
      901 aaagagattg tcctgcatat gccccgacta aaccagcgaa tacatttgcg atgattccca
      961 tagaactact tttttagtga ttttacctgc caagagacca ataagacctc cagcaataat
     1021 tgatcacaac actgcctacc ctcctttttt attttaatat caaaaaatca atttctttga
     1081 ttatt
//
LOCUS       DBERCA010000113         1084 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.113, whole genome shotgun sequence.
ACCESSION   DBERCA010000113 DBERCA010000000
VERSION     DBERCA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.113"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            30..1055
                     /locus_tag="ACL719_001976"
     CDS             30..1055
                     /locus_tag="ACL719_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS0699863.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT          425 a          149 c          184 g          326 t
ORIGIN      
        1 tagacttgaa atatgtttaa ggagttttta tgaaaaacaa attttttcta atagctattt
       61 tagctatgtg tatagttttt agcgcttgtt cttctaattc tgttaaaaat gaagaaaata
      121 cttctaaaga gcatgcgcct gataaaatag ttttagatca tgctttcggt caaactatat
      181 tagataaaaa acctgaaaga gttgcaacta ttgcttgggg aaatcatgat gtagcattag
      241 ctttaggaat agttcctgtt ggattttcaa aagcaaatta cggtgtaagt gctgataaag
      301 gagttttacc atggacagaa gaaaaaatca aagaactaaa tggtaaagct aacctatttg
      361 acgatttgga tggacttaac tttgaagcaa tatcaaattc taaaccagat gttatcttag
      421 caggttattc tggtataact aaagaagatt atgacactct atcaaaaatt gctcctgtag
      481 cagcatacaa atctaaacct tggcaaactt tatggagaga tatgattaaa attgattcaa
      541 aagccttagg tatggaaaaa gaaggtgatg agttaatcaa aaatactgaa gctcgtatat
      601 ccaaagaatt agaaaaacat ccagaaatca aaggaaaaat caaaggaaaa aaagtattat
      661 ttactatgat taatgctgca gatacatcaa aattctggat ttatactagc aaagatccaa
      721 gagcaaatta tttaacagat ttaggtctag ttttccctga atcattaaaa gaatttgaga
      781 gtgaagatag ttttgcaaag gaaatttctg cagaagaagc aaataagata aatgatgctg
      841 atgtaatcat aacttatggt gatgataaaa ctcttgaagc tttacaaaaa gatcctcttt
      901 taggtaaaat aaatgcaatt aaaaatggtg ccgttgctgt aattccagat aatacaccgt
      961 tagcagcctc atgcactcca acaccacttt caataaacta tactattgaa gaatacctaa
     1021 atcttttagg aaatgcatgc aaaaatgcga aataaaaaac aaataaacct aggcataatt
     1081 tttg
//
LOCUS       DBERCA010000114         1011 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.114, whole genome shotgun sequence.
ACCESSION   DBERCA010000114 DBERCA010000000
VERSION     DBERCA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.114"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1011
                     /gene="lanM"
                     /locus_tag="ACL719_001977"
     CDS             <1..>1011
                     /gene="lanM"
                     /locus_tag="ACL719_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="HGS0699864.1"
                     /translation="ILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFF
                     DDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQ
                     KKKLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQ
                     SLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKF
                     DIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLY
                     GLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQM
                     RL"
BASE COUNT          368 a          110 c          198 g          335 t
ORIGIN      
        1 aattttatca aatttagaaa atgattttag tgtgctagaa gaagaattag ggctaagggg
       61 gaaattaaat gatataaaat ttggaaaggg tgatacacac agccaaggaa aaactgtttt
      121 gatactcttc tttgatgacg cgaaaattgt ttacaagcct aaaaatttaa taatcaataa
      181 ctcactaaat actattgctg agtatatccg aaaggttgat gaaaaaatta ggataagaat
      241 acctcgaact attgcttatt cggatcacag ctatgaagaa tttattgatt atctacctct
      301 agagcaaaag aaaaaattac ctgaatatta ttataatttt ggtgtgcttt tagcatttat
      361 atatttattt aatgggagtg atatacattt tgaaaattta atttcctatg gagatatgcc
      421 tgtaataata gactttgaaa caatgttaca gcaacctcta tttgatgata aaactggtca
      481 gagtttgctg gataccctgt ttcacagggt gactcgaact ttgcttcttc caacagaggg
      541 agtaaaaagg gaggatggtc ttgatgttga gatgagtgct cttacaggaa attttaagaa
      601 ggatgctttt aatggtcaag ttttaattaa tttaaatact gataaggtaa agtttgatat
      661 tgggaaaatt gattttgaag gtgggaaaaa cctgcctgtt cgggatggtg atatagaatt
      721 tgataagtat ataaaagatt tcaaaaaagg ttttagggat ttttatctga tatttgaaga
      781 gttaaataaa acagaagaat ttaaaatgct attaaaagca aacttgtatg gtttaaagac
      841 aagggtactt tttagagaca caaatagcta tgcttcagta ctatcttttt tatatcatcc
      901 tgatttttat gaagaaatgt tagatagaga aaaagcattg gaaaatctct ggagcaataa
      961 gttttctaat caaggtattg tagcaagcga atgtgaacaa atgaggttat t
//
LOCUS       DBERCA010000115          997 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.115, whole genome shotgun sequence.
ACCESSION   DBERCA010000115 DBERCA010000000
VERSION     DBERCA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.115"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(83..>997)
                     /locus_tag="ACL719_001978"
     CDS             complement(83..>997)
                     /locus_tag="ACL719_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS0699865.1"
                     /translation="FILLIGDESYSFSTLIKVLNSETVPGASFSIMEIRLPKLLAGII
                     AGWSFGLAGFIFQTMLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCG
                     LTSSLILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGS
                     LSFAKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCA
                     VSLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPA
                     RYPVGVVTGLLGSPYLIYLLIKMNRRNI"
BASE COUNT          364 a          172 c          142 g          319 t
ORIGIN      
        1 tcaggtattt ttatatttat attttccaag attatttttt catcataacc gcaggtaaga
       61 ttatttgacc acagaccttt cattatatat tcctcctgtt catttttatt agtaagtata
      121 ttaagtatgg tgaacctaac aagccagtta caacacctac tggatatcta gctggtaaaa
      181 tattttgaga gaatatgtct gataacaaaa ctagtaaaat tccaaccaat ccagctaata
      241 ttgggcttct tttcttgcca atatttaagg ctatgggacc agctaaaaaa gatatacaag
      301 ctattggtcc tgtaattgaa gtagaaaaag cagttaaaga tacagcgcaa aaaattaaaa
      361 caagccttga aagctcggga tttgctccaa gtccgattgc tatttcttca ccaagttcaa
      421 taatttctag tcttttatta aaaaataaaa ctaatatagt agcaataata cttactatta
      481 gaacaagagg tatgtcatct aactttgcaa aagataaaga gccactgagc catctcataa
      541 cttcttgtaa ttcatatctt gctactttca acaataaaaa tgaggtgcct gctcttgtga
      601 cagcttgaaa accaatacct aatattatca gtcttgctgc tgaaaaacca tcttttttag
      661 ctagtaaaaa taatattaaa gatgatgtta gtccacaagt tattgaaata attccagtag
      721 ttaaactatt tgtttttaat accaatatgc aaaagaccgc tgcaatagat gaagaacttg
      781 tgacaccgat tatatcagga cttgcaagag gatttcttaa catagtttga aagataaatc
      841 ctgccaatcc aaaagaccag ccagctataa ttcctgctaa taattttggt aatctaattt
      901 ccataatcga aaaactagct ccaggaacag tttcactatt taagacttta atcaaagttg
      961 aaaaagaata actttcatct ccgataagta aaatgaa
//
LOCUS       DBERCA010000116          984 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.116, whole genome shotgun sequence.
ACCESSION   DBERCA010000116 DBERCA010000000
VERSION     DBERCA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.116"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(50..>984)
                     /locus_tag="ACL719_001979"
     CDS             complement(50..>984)
                     /locus_tag="ACL719_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS0699866.1"
                     /translation="SLKLGTKEINIRDFLAAFGMGNTNDDFIKSIIYKRIPRTIFAIL
                     AGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIGLSFLGISSSISHISFAIIG
                     GLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRFWQI
                     GSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSLAII
                     ASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDIIGR
                     MLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          345 a          186 c          139 g          314 t
ORIGIN      
        1 agtgttattc tattttttct tttttgaata cctataatta aattttgcat tagttattaa
       61 cccctctatt tttcatagtt acataaataa gtactggacc cccgattatt gcagtaatta
      121 tccctacttc aatttcacct ggtttaccta acatacggcc gattatatca catataagca
      181 agagctctgc acctataaaa gatgaagaaa tggtcattgt gcgtatatct ttgcttataa
      241 ataagccaca aaagtgagga actataagac ctacgaagcc aataggtcca ccaattgcag
      301 taatacttga acataaaagc acacttgcaa ttattgcaag tgatcttatc ctattaacat
      361 taactccaag accaacagcc atttcatcac ccatagctaa agcgtttaaa tctgatgaaa
      421 taaatatagc tatcaagtga cctaaaatta taaaaggtag tagtgtagat atagaagata
      481 atgtagctgc tccaaggcta cctatttgcc aaaatctaaa tttgtctaag acgttattat
      541 tcggtaaaat taaaaaactt acaaaactgc ttaaagccat actaacacaa gttcctgata
      601 aggcaagttt tataggggta aggcctgctt ttccgcttac agcaatcgcg tatacaaaaa
      661 ttgcacttac taagccacca atgattgcaa aacttatatg gcttatgctt gatgaaattc
      721 ctaaaaaaga aagaccaatt actacactaa gacttgctcc tgtgtttata ccgagtatac
      781 ctggatcagc tattgggttt ctagtaactg attgcatcaa tacaccgctt atggcaagac
      841 tagaacctgc taaaattgca aaaatagttc taggtattct tttatatata attgatttaa
      901 taaaatcatc atttgtatta cccattccaa aagctgctaa aaaatctctg atattaattt
      961 cttttgttcc aagctttaat gaca
//
LOCUS       DBERCA010000117          977 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.117, whole genome shotgun sequence.
ACCESSION   DBERCA010000117 DBERCA010000000
VERSION     DBERCA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.117"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..525
                     /locus_tag="ACL719_001980"
     CDS             <1..525
                     /locus_tag="ACL719_001980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0699867.1"
                     /translation="STNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDF
                     WTYSMGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIP
                     NNKKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICYLKC
                     RDNFRLLGEIYPVL"
     gene            579..863
                     /locus_tag="ACL719_001981"
     CDS             579..863
                     /locus_tag="ACL719_001981"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0699868.1"
                     /translation="MCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCA
                     CANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG"
     gene            856..>977
                     /locus_tag="ACL719_001982"
     CDS             856..>977
                     /locus_tag="ACL719_001982"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0699869.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQNYKNIEV"
BASE COUNT          362 a           85 c          200 g          330 t
ORIGIN      
        1 tcaactaatt attttttata tatgttaaaa aaacttgaca agaatggatt tggaaaggtt
       61 aagacagtga taacatgcca tggatgggtt gaatataact taaaaaagaa attcttgact
      121 tattttgatt tttggacata ttctatggga gatgctttta tttgtgtatc tgaaactatg
      181 aaaaaaagat tggagagtat aataaaaaat aagaagatag ttgcaataaa taacggaatt
      241 aatgtatcaa atagcgattt agatgtagta ggtgttcaag actttaaaaa agaattttgt
      301 attccaaata acaaaaaaat catttgttat gttggaaggt tggatccaga aaaaaggccg
      361 gatagattcc ttgaatttgc agaaaaatta tttttagtca gagaagatgt gatttttatt
      421 atggctggaa atggtagtat gtgggctgct ttgaaagaaa aaatttgcta tttgaaatgt
      481 agagataatt tcaggttact tggtgaaatc tatccagtac tatagtttac caaatatcgg
      541 atttattgta tattccttct gatacagaag gaatacctat gtgtgtttta gagtcaatgt
      601 cacaaggaac tccggtttta gctagtaatg ttggcgggtt aagtgaaatt attgaacata
      661 gggttgatgg atttttattt gagaaggaag atgttgaggg agtgtgtgct tgtgctaatt
      721 ttttactcaa tgattctgag tatttgaaat atataggtga gaatagtaaa tcaaaaataa
      781 gaaaacattt ttctgtgcaa aaaatgtttg tagaaaccat gagagtatat gatgaattat
      841 tagagaagag tagtcatgga taggaatata gatcaagaat tagtaagtat tataatacca
      901 acacataata ggtatgaatc tctgattaga gctgtgaaaa gttgtttaca tcaaaattat
      961 aaaaatatcg aagttat
//
LOCUS       DBERCA010000118          975 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.118, whole genome shotgun sequence.
ACCESSION   DBERCA010000118 DBERCA010000000
VERSION     DBERCA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.118"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>975)
                     /locus_tag="ACL719_001983"
     CDS             complement(<1..>975)
                     /locus_tag="ACL719_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HGS0699870.1"
                     /translation="LLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGIIFLDW
                     VVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFK
                     QLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQ
                     NEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFF
                     LNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIF
                     LKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEILKNLN"
BASE COUNT          340 a          171 c          128 g          336 t
ORIGIN      
        1 aatttaagtt ttttaatatt tctttatctc catacttata gcaaatattt tttgcttcat
       61 ataacggaaa atctctattc acctcatttg gttcgatatc attcatttta tttgactcaa
      121 ttggattaat tgaatacaat tttaaaaaaa taggcttcgt accaataata gaggataatt
      181 gacctcctaa ttcacctagc gctgtaaaaa taacacctgt tagtgctcct attgcttcaa
      241 tagtaccaat tttcactatt ccttttattg caagatagcc tgttaaaaaa acgagagata
      301 tctgaaaaaa aatattgaga aagaagctaa tagcgcctgc taacgtttct acagttgtct
      361 ttctttgtat aaccatcttt aataaaattc ctgcttcttt aattttctta ggcaatacat
      421 ataaaagatt caaggacgct aacacatcaa atccattcaa tatagtctca ctagatttta
      481 aaaaagcttc attttggtta gttaaattta gactaacttc tcgcattttc gatgcaaaga
      541 tttttggtac aagtagcata atcattaatg aaaacaaggt ggctacagtc aatgaccaat
      601 gatagtgatt aagagtcaca actgcaaata tagtaccaga aattcctttt attactaaaa
      661 aaagttgttt aaaagcctga tcatttaaag tctgaacatc attatttagc cacgaaagat
      721 atgttcctga tgatttacta tgaaattctt gataggtaga gttagagatg tctgtggcaa
      781 ctctatttcg aatctctaga ttaaactctt ggatcacttc aacctgataa tttttcacta
      841 cccagtcaag gaatattatc ccacaccaga caatcatttg gtagattgac aatttcaaaa
      901 accgctctaa attcatcgca attaattcat tcaacaccag agcattaata gttgctgcat
      961 aaattagcaa taatt
//
LOCUS       DBERCA010000119          975 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.119, whole genome shotgun sequence.
ACCESSION   DBERCA010000119 DBERCA010000000
VERSION     DBERCA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.119"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..742)
                     /locus_tag="ACL719_001984"
     CDS             complement(<1..742)
                     /locus_tag="ACL719_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699871.1"
                     /translation="MKDDQKYLLAGFYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFMFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSKVDLDIFYKVLPLLY"
BASE COUNT          376 a          169 c          125 g          305 t
ORIGIN      
        1 tataaagcaa aggtaaaact ttataaaaaa tatctaagtc aaccttactc tgattctgaa
       61 gcaagtagga gtcaagcaaa ttttcaaaaa tagctaacaa tatgattggt gtaaggtttt
      121 tgtattcaca tgtcaagtat tccgagtatc gtgcagatga tatttctttt acataacttt
      181 ttgaaagcaa gattttttca aattcatcat ctattattaa tcttctattt ccaatccaaa
      241 cggtattacc acccaaataa gattgtgcaa atagcaatat gtaagaaaac atgaacgtaa
      301 atactgaaag aggtagaatt ctatgcgtaa aaaaatagac gataatggta gccaggagta
      361 aagatatctc tcgtgacaat cgaattcttt tcagcatctt ttttaacagt ccgtaaggtt
      421 gtccttggca atattcttga actaaattaa tcggaatgac atctctcacc atttctggaa
      481 agtagagtaa gtttataggt tgaaatctcc agctcccatc ataagtaaat ggatatagaa
      541 ccaaatactt aaaagatttt tttcttataa acttaattat cacaatatga atgactgttc
      601 caatgaagta tattaaatat aaaagtaaaa ccgccatcaa tatacttaca aaaatcccct
      661 tggaatcaat caatggaaaa taaaatattg caactagaag cgaataaaat cccgctaata
      721 aatatttctg atcatctttc attcaaaaca ccatattatt tactaagaat acatcaatac
      781 aatagagcaa ctctgtaagt caatatttga taaagcaaag ctttaaaccc ttccccagac
      841 gttagctttt attcaaccca ctacagttga caaggagccc aaattattga taaaaacttt
      901 agtcatcatc gtctctataa aaatattttc tatacactct tgataatacc aaaattaatg
      961 ccaatatcat accaa
//
LOCUS       DBERCA010000120          956 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.120, whole genome shotgun sequence.
ACCESSION   DBERCA010000120 DBERCA010000000
VERSION     DBERCA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.120"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>956
                     /gene="phtD"
                     /locus_tag="ACL719_001985"
     CDS             <1..>956
                     /gene="phtD"
                     /locus_tag="ACL719_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HGS0699872.1"
                     /translation="PAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETA
                     AGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEA
                     LDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKY
                     TTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPS
                     TGHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNI
                     KFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          308 a          187 c          215 g          246 t
ORIGIN      
        1 accagctcca agcaatccaa ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg
       61 cgatggttat gtctttgagg agaatggagt ttctcgttat atcccagcca aggatctttc
      121 agcagaaaca gcagcaggca ttgatagcaa actggccaag caggaaagtt tatctcataa
      181 gctaggagct aagaaaactg acctcccatc tagtgatcga gaattttaca ataaggctta
      241 tgacttacta gcaagaattc accaagattt acttgataat aaaggtcgac aagttgattt
      301 tgaggctttg gataacctgt tggaacgact caaggatgtc tcaagtgata aagtcaagtt
      361 agtggatgat attcttgcct tcttagctcc gattcgtcat ccagaacgtt taggaaaacc
      421 aaatgcgcaa attacctaca ctgatgatga gattcaagta gccaagttgg caggcaagta
      481 cacaacagaa gacggttata tctttgatcc tcgtgatata accagtgatg agggggatgc
      541 ctatgtaact ccacatatga cccatagcca ctggattaaa aaagatagtt tgtctgaagc
      601 tgagagagcg gcagcccagg cttatgctaa agagaaaggt ttgacccctc cttcgacagg
      661 ccatcaggat tcaggaaata ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa
      721 agcagctaag aaggtgccac ttgatcgtat gccttacaat cttcaatata ctgtagaagt
      781 caaaaacggt agtttaatca tacctcatta tgaccattac cataacatca aatttgagtg
      841 gtttgacgaa ggcctttatg aggcacctaa ggggtatagt cttgaggatc ttttggcgac
      901 tgtcaagtac tatgtcgaac atccaaacga acgtccgcat tcagataatg gttttg
//
LOCUS       DBERCA010000121          939 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.121, whole genome shotgun sequence.
ACCESSION   DBERCA010000121 DBERCA010000000
VERSION     DBERCA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.121"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..813
                     /locus_tag="ACL719_001986"
     CDS             85..813
                     /locus_tag="ACL719_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HGS0699873.1"
                     /translation="MIPKKIHYIWFGGSEKPDVVLKCINSWKKYMPDYEIVEWNEDNF
                     DLSDSQFAKSAYESRKWAFASDYARFKILSKYGGIYFDTDVELLKTIPDDILAHSSFT
                     GFEYIGEVNPGLVYACMPDDKIAKYMVQYYEQASFDINHLVTVNTIITDYLLKNNFQK
                     NNQFQIIDGLAIYPDDYFCGYDQEVKEVRLTERTISIHHYSATWKTRTLKRKVQMIVK
                     TIIGAENYRKLLYCKRKITNAVSK"
BASE COUNT          340 a          105 c          167 g          327 t
ORIGIN      
        1 atactaagag aaaatttgct ttcaagttgg aaaatatcaa tactaaatcg gtttaatatt
       61 attaaagaat gaggagaata ttatatgatt cctaaaaaaa tccactatat ttggttcggt
      121 ggaagtgaga aaccagatgt tgtcttaaaa tgtattaatt cttggaaaaa atatatgcca
      181 gactatgaga ttgttgaatg gaatgaggat aattttgatt tatcagattc acaatttgcg
      241 aagtctgcat atgaatctag gaaatgggca tttgcatcgg attatgctcg ttttaaaatt
      301 ttatctaagt atggtgggat ctattttgat acggatgttg agttgttaaa aacaatacca
      361 gatgatatat tagctcattc atcttttaca ggttttgaat atattggtga agtaaatcca
      421 ggacttgtat atgcttgtat gccagatgat aagatagcaa aatatatggt gcaatattat
      481 gagcaagcaa gttttgatat caatcatttg gtaactgtca atacaattat tacagattat
      541 ctattaaaaa ataactttca aaaaaataat cagttccaaa taattgatgg attagcaatt
      601 tatccagatg attacttttg tggttatgat caggaggtca aggaggttag gcttacggaa
      661 cggacgatta gtattcatca ttatagtgct acttggaaaa caagaacttt aaaaagaaaa
      721 gtacagatga ttgttaaaac aattatagga gctgagaatt ataggaaatt attatattgt
      781 aagaggaaaa taaccaatgc cgtatctaaa taatgtaccg caaaataaat tttctataag
      841 attatttgat attgctatag ttttattgac tttaaagatg ttagtttctt cgattccagt
      901 ttttgacttt gtgtttcctc aaaaatttca aaatatcct
//
LOCUS       DBERCA010000122          936 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.122, whole genome shotgun sequence.
ACCESSION   DBERCA010000122 DBERCA010000000
VERSION     DBERCA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.122"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..543
                     /locus_tag="ACL719_001987"
                     /pseudo
     CDS             1..543
                     /locus_tag="ACL719_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          332 a          110 c          185 g          309 t
ORIGIN      
        1 tttaggtgaa agtggaagcg ggaaatctac attattaaaa ttattgaatg gctttttgag
       61 agattatagt ggagaattgc gattctgcgg ggatgatata aaaaaacctc ctatttaaat
      121 atggtttcga atgttctata tgtagatcaa aaagcttatt tgtttgaagg tacgattaga
      181 gataatattt tattggaaga aaattatact gatgaagaaa tactacagtc tttagagcaa
      241 gttggtttga gtgtaaaaga ttttcctaat aacattttag attattatgt tggtgatgat
      301 gggagattac tgtcaggagg gcagaaacaa aaaattactt tagctagagg gctaattaga
      361 aataagaaaa tagtattaat tgacgaggga acttctgcta tcgataggag aacttcgtta
      421 gcgattgaac gtaagatatt agatagagag gatttgactg tcattattgt tacccatgct
      481 ccgcatccgg aacttaaaca atattttact aagatatatc aatttccaaa ggattttatt
      541 taagaatata ggggagggaa acatgagatt ctattgtgaa gcttatgagg taattactga
      601 agagaaaaaa gtatcatttt tttatgtaga tgagaaaccc ttaaggtatc tgttatttga
      661 ctatatattt ctactagttc tagctaaaaa aaaatatccc aatcttatga actgcacccc
      721 aaaagttaga tttattctgt ctaacttttg gggtgcagtt cattttgcat agttttggca
      781 ttcatcctag atatccatat aaaatgtctg agattgttga aatgtctaaa aaaatatcag
      841 atctaaacga taaaaaagaa tttcacaaaa agaactgtga gttgaggttt caagatagtg
      901 ttcttgataa cataaatagt gtcccgggta caactc
//
LOCUS       DBERCA010000123          914 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.123, whole genome shotgun sequence.
ACCESSION   DBERCA010000123 DBERCA010000000
VERSION     DBERCA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.123"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            78..828
                     /locus_tag="ACL719_001988"
                     /pseudo
     CDS             78..828
                     /locus_tag="ACL719_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            841..>914
                     /locus_tag="ACL719_001989"
     CDS             841..>914
                     /locus_tag="ACL719_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGS0699874.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKV"
BASE COUNT          314 a          105 c          182 g          313 t
ORIGIN      
        1 aaggtgaatt tttttgatga tgtggaattt aatcagacac ttagtgagtt tttagataaa
       61 ttgtagaggg aatggttatg agtaacaata ctaagtatat ctttttatct ccacatttag
      121 atgacgcaat tttttcctgt ggtgattata tttcaaaatt aacttcagaa ggagaaattg
      181 tacttgttat tactatattt tctggttacc ctttgagcca acagctacag ccctctgcta
      241 agcaatttca taagttgtgt aaccttggga aatatccgat tgaagaaagg aagaaagaag
      301 acaggttagc ttgtgaacgt ctacaatgtg attttagaca cttatcgtac tatgaatgtc
      361 tctataggaa agatagaaat ggcaattttc tataccgtca tatctatagt gaattaaaaa
      421 atgaggacac attaaagaat gatatcataa aagagctgtt aatgcattta gacgacaaat
      481 gtgtcgtata ttgtccgctt tcacttggtg accatgttga tcatgttttt gtgaatagta
      541 ttggaagggc gcttgaattt atgcgttata aagtaatata ttatgaggac ttccaatatg
      601 taagtgatag tagtatggtt ggttatatgg gaaaaactaa agaattaaaa atgtatcaag
      661 aggaacttga taaaaaaaca ttatattgat agaatatcat caatatcgtg ctataaatct
      721 cagattttga taatctggaa atcagttgaa aaacttttaa acaatattaa agagttgtat
      781 ttaaggaatg gggcagctta ttctattaga ttttggatta agaaataagg aagtagtttt
      841 atgaatatag cagtaattgg tttggggcat gttgggctgg cctacgcgtt actatttgca
      901 tctaaatata aagt
//
LOCUS       DBERCA010000124          884 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.124, whole genome shotgun sequence.
ACCESSION   DBERCA010000124 DBERCA010000000
VERSION     DBERCA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.124"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(127..489)
                     /locus_tag="ACL719_001990"
     CDS             complement(127..489)
                     /locus_tag="ACL719_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS0699875.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYLWA"
BASE COUNT          308 a          176 c          110 g          290 t
ORIGIN      
        1 agatttattt tagtatatcc aatcactagt ccaataattg gactggtaga catgcttaga
       61 tataataaaa ataacaaaaa atgaatttca ccaattcctg caaaaaaatg tttcatagct
      121 atagttttac gcccataaat atccatcaaa tttaccgtct acgatatcta aaccatatgc
      181 aatggctcct cctaatgaga agacttcagc aacagtagcc gcaatattaa gagcattagt
      241 aatagttgcc atactaactc caccaatata tcttgttata gcacttctaa cgactcggct
      301 catcatacca cgattagcaa ttaacttttt aatggcatat gtctggtata ctgacatccc
      361 tccagaaact gcagctgcag caatattatc acgtttgttc caccagttac aaccaacacc
      421 acccccatca agatacatca tctcttcttg ctcaagaaca acataattat ttggtaatac
      481 gagttccatt tattttctcc ttttttatta agcatagtta ggatagtttc ctaacaagtg
      541 atcgttgact ttgtttatag cacttagtta cctagataag atatccttta gcttgcttcc
      601 aaaatttaag tctgcaccta taatatgagt tcatattagt actgctaaat cctatctata
      661 gtaatgtcaa acacctaaat aaagtcatct ctccgattca ttcaagaaat gaagaaaggc
      721 aaaaaatctt taaaataaga aaaatgcata ttatcaggtg ttcaaaaata ttgatgatat
      781 acgttatata gatagaaaga tttactcaat ctcattttca aattgagttt tttcccaacc
      841 tcgtttttaa tttatatctt caatagaatc taatgaccga gcta
//
LOCUS       DBERCA010000125          876 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.125, whole genome shotgun sequence.
ACCESSION   DBERCA010000125 DBERCA010000000
VERSION     DBERCA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.125"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..110)
                     /locus_tag="ACL719_001991"
     CDS             complement(<1..110)
                     /locus_tag="ACL719_001991"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0699876.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKIPSGLYKIDTGKV"
     gene            complement(235..421)
                     /locus_tag="ACL719_001992"
                     /pseudo
     CDS             complement(235..421)
                     /locus_tag="ACL719_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845777.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            382..>876
                     /locus_tag="ACL719_001993"
     CDS             382..>876
                     /locus_tag="ACL719_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0699877.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTDEKEVNHLI
                     KYAMFDKEQKRGYKQSARNLANWHYNDKEDSYTHPDGWYYRFHHTKYQKTQTDFQQEI
                     KVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQGRQIFAQRKIDVEPVFGQIKA
                     SLGYK"
BASE COUNT          291 a          179 c          152 g          254 t
ORIGIN      
        1 acttttcctg tatcaatctt atataatcct gatggtattt ttgataacgt actttttcct
       61 gaccccgatt gaccgactag tgcaattctc tgtcctggct caatagtcaa atttaaattt
      121 tctattacag gagctgagtc tttggaataa gagaaagata ggtccttaat atctattcta
      181 cttcaaacgt ttctaaacta tactccggaa aataatttaa ccaataacta tctttcatcg
      241 tatcctccaa ccagtactaa gagtctggga caatagtctc ttatctccaa aaaaaagcgg
      301 atttgccgtt gcttttttgc atggttgcga tagtcttggt aaaatagaat tgcccaataa
      361 accatttaga aaggctatcc catgcatatt cactataaca caaatcaaac aactttacca
      421 ctagaaatca gttctttctt gccgcaagac catctcgtct ttactattga aaaagtggtg
      481 aataccgatg aaaaggaggt aaaccatctg attaaatatg ccatgtttga taaggaacaa
      541 aagagaggct ataaacagtc agctagaaac ttagcgaatt ggcactataa tgacaaggag
      601 gatagctata cacatcctga tggctggtat tatcgttttc accataccaa atatcagaaa
      661 acacagacag actttcaaca agaaatcaag gtttactacg ccgacgaacc tgaatcagcc
      721 cctcaaaagg gactgtatat gaacgaacgc tatcaaaact tgaaagctaa agaatgtcag
      781 gcgcttttat ctccccaagg tagacagatt ttcgctcaac gcaagattga tgtggaacct
      841 gtctttgggc agataaaggc ttctttgggt tacaag
//
LOCUS       DBERCA010000126          851 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.126, whole genome shotgun sequence.
ACCESSION   DBERCA010000126 DBERCA010000000
VERSION     DBERCA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.126"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(187..258)
                     /locus_tag="ACL719_001994"
     CDS             complement(187..258)
                     /locus_tag="ACL719_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HGS0699878.1"
                     /translation="MKKISKFFPILMLVMDIIIIVGG"
     gene            340..>851
                     /locus_tag="ACL719_001995"
     CDS             340..>851
                     /locus_tag="ACL719_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HGS0699879.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTKTESFANLIQYIRKQYSLQNINNIQSLLSDS
                     SNYTLDSFEKTMVKSILHTMDSRIIPSDDELLQLADYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLT"
BASE COUNT          304 a          143 c          107 g          297 t
ORIGIN      
        1 tattttttcg actgtttcag gatttttaat ataccccgag tcattatacc ataccctata
       61 attaatactt ttttatgttt caaagtctca ccactctcta aaattttacg atttaactta
      121 aatatgtttt agctagtaag tatagtcttt agatactcta aagacttagg tgtacaccta
      181 agtgatttaa ccaccaacaa taatgataat gtccatcact agcatcaaaa ttgggaaaaa
      241 ttttgaaatt ttcttcatat ttagacttcc tttcaatttt tatttattgt acaataaata
      301 aaactaagtt aactttttgt tgggagaatg ggaaaaacta tgaaatcaaa acttggcatc
      361 acacttagaa aagttcgtaa aggcaaacaa atcagtctat gctctgttgc tgatgagcat
      421 ctttcaaaat cgcaaatatc ccgatttgaa cgtggagagt cagagatatc atgtatccga
      481 ctgatcaata ttttagacaa attacacatt actttggatg agtttcttat tttacacgat
      541 gaagactata caaaaactga gtcatttgcc aacttaatac agtatatccg taaacaatat
      601 tcattacaaa acatcaataa tatacaatct ctactttcgg attcctcaaa ttacacttta
      661 gattcttttg aaaaaacaat ggtaaaatcc attttacaca caatggattc aagaatcatt
      721 ccatcagatg atgaattact tcaactagca gattatctct ttaaagttga aaaatggggt
      781 tattatgaga ttattttatt aggtaactgt gtacgaacaa ttaactacaa ctcctacttc
      841 ttattaacta a
//
LOCUS       DBERCA010000127          840 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.127, whole genome shotgun sequence.
ACCESSION   DBERCA010000127 DBERCA010000000
VERSION     DBERCA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.127"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..684
                     /locus_tag="ACL719_001996"
     CDS             <1..684
                     /locus_tag="ACL719_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0699880.1"
                     /translation="NGCGKSTLIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAI
                     LPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEE
                     LSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMIL
                     HDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMI
                     PIGKHHVSS"
BASE COUNT          322 a          144 c          137 g          237 t
ORIGIN      
        1 aatggttgtg ggaaatcaac actcattaaa accttgtctc gacttataaa gccattagag
       61 ggagaagtat tgcttgataa taaatcaatt aattcttata aagaaaaaga tttagcaaaa
      121 cacatagcta tattacctca atctccaata atccctgaat caataacagt agctgatctt
      181 gtaagccgtg gtcgtttccc ctacagaaag ccttttaaga gtcttggaaa agatgacctt
      241 gaaataataa acagatcaat ggttaaggcc aatgttgaag atctagcaaa taacctagtt
      301 gaagaacttt ctgggggtca aaggcaaaga gtatggatag ctctagccct agcccaagat
      361 acaagtatcc tacttttaga tgagccaact acttacttgg atatctcata tcaaatagaa
      421 ctattagacc tcttgactga tctaaaccaa aaatataaga caaccatttg catgattttg
      481 cacgatataa atctaacagc aagatacgct gattacctat ttgcaattaa agaaggtaaa
      541 cttgttgcag agggaaagcc tgaagatata ctaaatgata aactagttaa agatatcttt
      601 aatcttgaag caaaaattat acgtgaccct atttccaatt cgcctctaat gattcctatt
      661 ggcaagcacc atgttagctc ttaataattg aatatcggaa ttcatttaaa agtcgcaaat
      721 tttgaactga ccctcaaaag ttaggccaaa aaactaacta aaggaggtca gttttttaat
      781 ggcaaataca gcacagaatt taagataaaa ttagtaaaag aatatcatga atgaaaaata
//
LOCUS       DBERCA010000128          818 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.128, whole genome shotgun sequence.
ACCESSION   DBERCA010000128 DBERCA010000000
VERSION     DBERCA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.128"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            182..637
                     /locus_tag="ACL719_001997"
     CDS             182..637
                     /locus_tag="ACL719_001997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699881.1"
                     /translation="MIVLYDSFDSIVNCFDKLKGQYILLPVAFESSIKNYSWKDFNFE
                     YHKRIEIVDSFHSFTKGMVLIENCNYKINRAIIQPATEILMLNYLKKKSMDLRIDFTH
                     SKVMANKKFLEEGYRFSIVSQETIINNDDIIICETFSPEMIWCLYRVKG"
BASE COUNT          298 a           76 c          160 g          284 t
ORIGIN      
        1 aaatgtaaaa acttctgaga atatttattt agagatgagg gatttagtat ctttggaaaa
       61 tcaaggatta gactttgaac attgtatttt gagcttgggg gaaagatgat aataaataca
      121 ttaggaccaa ctgcaacaga ttgtttttta tgcaataaat cgtataaaat taaaagtaga
      181 tgtgattgta ctgtatgata gttttgatag tatagttaat tgctttgata aattaaaagg
      241 gcaatatata ttattacctg tagctttcga gagtagtatt aaaaattata gttggaaaga
      301 tttcaacttt gaatatcata agcggattga aattgtggat tcttttcact cttttactaa
      361 gggaatggtt ctaatcgaga attgtaatta taaaattaat agggcgatta tccaacctgc
      421 aactgagatt ttgatgttaa actatttaaa gaaaaaaagt atggatctga ggatagattt
      481 tacccatagt aaagttatgg caaataaaaa atttctggaa gaaggttatc gtttttcaat
      541 agtaagtcaa gaaacgataa ttaataatga tgatattatt atttgtgaaa ctttctctcc
      601 agaaatgatt tggtgcttat atagagtgaa aggatagaaa atgttttctg aaattattaa
      661 gaaatatgat attgttacac tgaaagatag tggttttttg agaggtgatt ggttcatagg
      721 ttcagcgtta atggctcatg actatgaaag ccttaaggaa gggatatatt tctatcccca
      781 ggaaacaatt tggacaagaa tcttggattt agaatata
//
LOCUS       DBERCA010000129          760 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.129, whole genome shotgun sequence.
ACCESSION   DBERCA010000129 DBERCA010000000
VERSION     DBERCA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.129"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            40..210
                     /locus_tag="ACL719_001998"
     CDS             40..210
                     /locus_tag="ACL719_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="HGS0699882.1"
                     /translation="MRKKRGIKKLVTFALLGVFMFSNTIPYQQFIQKNKQLEIRVQSQ
                     KKSNGLDVGKAD"
     gene            313..537
                     /gene="pneA1"
                     /locus_tag="ACL719_001999"
     CDS             313..537
                     /gene="pneA1"
                     /locus_tag="ACL719_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HGS0699883.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            544..732
                     /gene="pneA2"
                     /locus_tag="ACL719_002000"
     CDS             544..732
                     /gene="pneA2"
                     /locus_tag="ACL719_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HGS0699884.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          273 a           94 c          156 g          237 t
ORIGIN      
        1 ttagaataaa aacaaggaaa aagaaagggg tttcattgca tgagaaaaaa acgtggaatt
       61 aaaaaattag ttacatttgc attgctaggt gtttttatgt ttagtaatac aattccttac
      121 caacagttta ttcagaagaa taaacaattg gagattcgag tgcaatcgca aaagaagtcc
      181 aatggtcttg atgttgggaa ggcggattaa aacccgatat tttgcacgag aatgagagaa
      241 ttataattta tctgaaaaaa cgaaaaatat agagcaactt atttaacttt ttataaggag
      301 gagttattac ttatgaccaa ctttaattca aacgaaaaat tttgtggaaa aagtttaaaa
      361 tcattgtctg ctgatgaaat gagtttaatt tatggtgcat ctgatggtgc agagccacgt
      421 tggacgccta ctcctattat tttgaaatca gctgcagcta gctcgaaagt atgcattagt
      481 gcagctgttt ctggtattgg ggggctagtt agctacaata atgattgttt ggggtaaatt
      541 aaaatgaaaa atgattttgt aatcggaaag agtttgaaag aattatctct tgaagaaatg
      601 caattggttt atggtggcac agatggtgct gaccctagat caacaattat atgttctgct
      661 acattaagtt ttattgcatc atatttgggg tcagctcaga ctcgttgtgg caaagacaac
      721 aagaaaaaat aaaatattca attttaaaaa taaaagttgc
//
LOCUS       DBERCA010000130          756 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.130, whole genome shotgun sequence.
ACCESSION   DBERCA010000130 DBERCA010000000
VERSION     DBERCA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.130"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..360
                     /locus_tag="ACL719_002001"
     CDS             <1..360
                     /locus_tag="ACL719_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HGS0699885.1"
                     /translation="TKFKLIYNSWGAYLLYSIKLFNPLQYDFSPKSLEVANTIVCNDG
                     WMSLVCNSKLEQINDQDFDDIHFRVEYMVKDLEYANFSVFHDEVKEINYLLQQYRDCL
                     VTDSGKDYTVIGKYDNL"
     gene            347..>756
                     /locus_tag="ACL719_002002"
     CDS             347..>756
                     /locus_tag="ACL719_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS0699886.1"
                     /translation="MITYRNDLLSKELDILISFFKKCEVGKISLVITGSLARGNPRIK
                     DGKLESDIDILVIVDSIQQLISIKKTLEGRFHFVHKISLIFCLKERINRSRYRGIINS
                     IRSVDNLLVDNLHIKNQIIEALDSPTNIVEQTRS"
BASE COUNT          276 a           87 c          140 g          253 t
ORIGIN      
        1 acaaaattca agttaattta taactcgtgg ggagcgtatt tgctttactc tattaagcta
       61 ttcaaccctt tacagtatga tttttcacca aagtctctag aggtagcgaa tacaatcgta
      121 tgtaatgatg gttggatgag tttagtatgc aactccaaat tagaacagat caatgatcaa
      181 gattttgatg acatacactt tagagtagaa tatatggtaa aagatttaga atatgctaat
      241 ttttcagtct ttcatgatga agtgaaagaa ataaattatt tattacagca atatagggat
      301 tgcttagtaa ctgatagtgg aaaagattat acggtaatag gaaagtatga taacttatag
      361 gaatgacttg ctgtctaaag aattagatat actaatttca ttttttaaaa aatgcgaggt
      421 aggaaaaatt tcactcgtta ttactggttc cttggcaaga ggtaatccaa gaatcaagga
      481 tgggaaatta gaatcggata ttgatattct agttatagta gacagtattc agcaattaat
      541 tagtataaaa aagactttag agggaaggtt tcactttgta cataaaattt ctttgatttt
      601 ttgtttgaag gaaagaataa atcgaagtcg ttatcgtgga attattaata gtattagaag
      661 tgtagataat cttttagtag ataatctgca tataaaaaat caaataatag aagctttaga
      721 ctcacctacc aatattgtgg agcagactcg ctctat
//
LOCUS       DBERCA010000131          738 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.131, whole genome shotgun sequence.
ACCESSION   DBERCA010000131 DBERCA010000000
VERSION     DBERCA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.131"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>738
                     /locus_tag="ACL719_002003"
     CDS             <1..>738
                     /locus_tag="ACL719_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS0699887.1"
                     /translation="NLEKQLDIIEIYFPSFDQRKKGYKLNLSNRKKLPNESLVTILHN
                     EVENIADSLVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFMYYVKLL
                     KDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLACLNYIK
                     SVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLGDTLYND
                     VSERDNSNFGYSFGHGLSGVIFTLKNI"
BASE COUNT          267 a           87 c          128 g          256 t
ORIGIN      
        1 aaaatttaga aaagcaactt gatatcattg aaatttattt tccaagcttt gatcagcgaa
       61 aaaaaggata taaattaaac ttatctaata gaaaaaaact cccaaatgaa agtttagtta
      121 ctattctgca taatgaagta gaaaatattg cagactcttt ggttattaat aatatttatg
      181 acgaatatcc aatttggagt atacctgtta attttaaagg ttctaaatgg tgtcttgaac
      241 aaacgggtaa tgatatgtat aggggtagag ctggaatatt actgtttatg tactatgtaa
      301 aattattgaa agataaatca tcatatactc aactgtacaa taaaatgtta tctgcaattc
      361 cgccatctgt ttctttatct aagaccacat ttggtttaac tggggatata ggatatttca
      421 ttgttctatc cattatagag gattacgata caaatactct cgcatgctta aattatataa
      481 agagtgtttt aactgagctt gaaaagactg attttgcaag tgtttcaaac aagtctgact
      541 atattaatgg tcttctacct ttaattaata cactggtaag atattatagg agaggaatag
      601 agaaggaacg tgttctaagg ctagtattaa gtttaggtga tacgctatat aatgatgtaa
      661 gcgagagaga taatagtaat tttggatata gttttggcca tggactatca ggtgtaattt
      721 ttacattaaa aaatattt
//
LOCUS       DBERCA010000132          692 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.133, whole genome shotgun sequence.
ACCESSION   DBERCA010000132 DBERCA010000000
VERSION     DBERCA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.133"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            39..440
                     /locus_tag="ACL719_002004"
     CDS             39..440
                     /locus_tag="ACL719_002004"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS0699888.1"
                     /translation="MTVINKFVLMEQAKKVLKNAYCPYSKFPVGAAILFKDGKVITGA
                     NIENVSFGVTNCAERSAIFYGASQGYRKGDILAIAVAGETEDYLPPCNICRQVMVEFC
                     EPDTLVFLLTRSYPQTKDSCSLERSLGSKFG"
     gene            373..519
                     /locus_tag="ACL719_002005"
                     /pseudo
     CDS             373..519
                     /locus_tag="ACL719_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT          242 a           99 c          120 g          231 t
ORIGIN      
        1 tagtgtgagt aaaatataat aataaaggag ttaaaacaat gacagtaatt aataaatttg
       61 ttttaatgga gcaagcaaaa aaagttttaa aaaatgctta ctgtccgtat tccaaatttc
      121 ctgtaggtgc agcaatttta tttaaagatg gtaaagtaat tacaggagca aatatagaaa
      181 atgtatcgtt tggtgtaact aactgtgcag aacgtagtgc tattttttat ggggcttctc
      241 aaggatatag aaaaggagat attcttgcga ttgctgttgc aggtgaaact gaagactact
      301 taccaccatg caacatttgt cgtcaagtta tggtagaatt ttgtgagcct gataccttag
      361 tctttctatt aacaagaagc tatcctcaaa ccaaagattc gtgcagcctt gaaagaagtc
      421 ttggttccaa gtttggctga gaacacaaga ttagaatttg ctcatcatca aaacactgct
      481 ggtatgcttg gagcctacta tcattttaaa ataaaataaa tttaatattt actttactaa
      541 attagtgtat tcttcctact ttaattagag aatgatttaa gttctcttta aaaatcaaaa
      601 gagtctagga caaaatagtt ctcaatcaca aaaaagctaa agatttccaa ttgtggaact
      661 ctagcttttt aattttgagt cgttctctta tt
//
LOCUS       DBERCA010000133          664 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.134, whole genome shotgun sequence.
ACCESSION   DBERCA010000133 DBERCA010000000
VERSION     DBERCA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.134"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            46..664
                     /locus_tag="ACL719_002006"
                     /pseudo
     CDS             46..664
                     /locus_tag="ACL719_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT          262 a           81 c          128 g          193 t
ORIGIN      
        1 atcgtctttt tctttgcaaa tttttaagaa gggaggtgat cttgcatgga ctttgaatat
       61 ttttataaca gagaagcgaa aagatttaac ttcttaaaag taccggagat attagttgat
      121 agagaagaat ttcggggctt atcagcagaa gcaattatcc tttattccat acttcttaaa
      181 tagacaggaa tgtcatttaa gaataactgg atagacaagg aaggcagagt atttatctat
      241 tttactgtcg aagaaattat gaaaagaaga aatatctcaa agccaactgc cataaaaaca
      301 ttagatgagc ttgatgtaaa aaaaggaata ggactgatcg aaagagtaag gcttggactt
      361 ggtaagccga acatcattta tgttaaagac tttatgagta tatttcaggt aaaagaaaat
      421 gacttacaga agtcaaaaaa cttaacttca gaagtaaaag attttaacct cagaagtaaa
      481 gaaaatgaac ttcaagaggt taagaacctt gactctaact atatagagaa taataagagt
      541 aagtatagta agagagaata tagttttggt gaaaacggac ttggaacatt tcaaaatgtg
      601 tttttagctg ctgaagatat atcggattta caaatcataa tgaactcaca gcttgagaat
      661 taca
//
LOCUS       DBERCA010000134          661 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.135, whole genome shotgun sequence.
ACCESSION   DBERCA010000134 DBERCA010000000
VERSION     DBERCA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.135"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..661
                     /locus_tag="ACL719_002007"
                     /pseudo
     CDS             1..661
                     /locus_tag="ACL719_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          257 a           68 c          130 g          206 t
ORIGIN      
        1 taattttaag taatgaagat ttaggtgcaa ctaatgccag aaacataggt atcaaaaatt
       61 ctagaggtaa gtatatatca tttttagacg atgatgatga atatatgcca gatcgaattt
      121 taaagttgat ggcttgtttt aaaaagagta gaatgaagaa tttagcttta gtttatagtt
      181 atggaataat aatttatcca aatggtacac gagaagagga gaagaccgat tttgttggaa
      241 atcccttgtt tgttcaaatg gttcacaata tagcaggtac gtcattttgg ttgtgtaaaa
      301 aagaggtgct agaattaatt aatggttttg agaaaataga ttcacatcag gacggtgttg
      361 ttttattaaa actacttgct caaggatacc aaattgatat agtgcgagaa ttcttggtga
      421 attactacgc tcacagtaaa gaaaacggta tcactggagt gacacaaaaa aacaattaat
      481 gcagatgaag aatattataa ttactgtagg aaatatttta atttattgag tttcaacgag
      541 agaatattgg ttacaaagaa atattattct ttaaacataa agcggttact attaatagga
      601 gacaaatgca aggctttaaa agtaatcaag aaggcaagag aagaaaaaat ttttaacgaa
      661 t
//
LOCUS       DBERCA010000135          642 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.136, whole genome shotgun sequence.
ACCESSION   DBERCA010000135 DBERCA010000000
VERSION     DBERCA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.136"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>642
                     /locus_tag="ACL719_002008"
     CDS             <1..>642
                     /locus_tag="ACL719_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0699889.1"
                     /translation="KKDAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTAS
                     KIQDILSVAPRSIGTTSPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGN
                     RLEAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLR"
BASE COUNT          195 a          138 c          127 g          182 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactggc tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgaa aagcattcag aatataccta ccaactcttg acatgctacc
      241 ctaatcctca gaaaaggctt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa aattcaggat atcctctcag ttgcacctcg ttctatcgga acaacatctc
      361 ctgctcgtga attcgaaatc atcaaacatt acaagaggct cattgacaag gcggaaacat
      421 gtgtcaatga cttgatggct gagttcaact cggtcatcac gacggtcact gggattggga
      481 atcgtttaga ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg
      541 ctcaattaca agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt
      601 tagctggaag aatgatcaaa cggggttccc ctcatctgcg gt
//
LOCUS       DBERCA010000136          622 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.137, whole genome shotgun sequence.
ACCESSION   DBERCA010000136 DBERCA010000000
VERSION     DBERCA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.137"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..511
                     /locus_tag="ACL719_002009"
     CDS             <1..511
                     /locus_tag="ACL719_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0699890.1"
                     /translation="VYITETVGGIRMSRTYINIRLAYETKYWLECIQEEVQKKLDALI
                     NEQDIEELESIITNKLEKKDSLLGAVSITTFFKVSSSSVIEKAYNETKNYTTSEWREI
                     ARQMNLAKVKQNMEIGTLTPRLYLDNNIISELEKYQFLFKSDDMIRQVRMSYVIKMVA
                     FAYYKKLFA"
BASE COUNT          243 a           74 c          115 g          190 t
ORIGIN      
        1 tgtctatata acagagacag ttggaggtat aagaatgagt cgtacttata taaatataag
       61 acttgcatac gaaaccaagt attggttaga atgtatacaa gaagaggttc aaaaaaaatt
      121 agatgcttta attaatgaac aagatataga ggagttagaa tcaatcatca ctaataagtt
      181 agaaaaaaaa gattctctat tgggggctgt ttccataaca acttttttca aggtatcttc
      241 ttcaagcgtg attgaaaaag cctataacga gacaaaaaat tacactactt cagaatggcg
      301 agaaattgca aggcaaatga atttggcaaa agttaagcaa aacatggaaa ttgggacgtt
      361 aacacctaga ttatatttag ataataatat tatttcagaa ttagagaagt atcaattttt
      421 attcaaaagt gatgatatga taaggcaggt aaggatgagc tatgttatta aaatggtcgc
      481 atttgcctac tataaaaaat tgtttgcatg atttataagc tcgataataa gagcttataa
      541 aaatacaacc tttgtctgtg ttaacgagac ttttaaactt tttaatcgga aggagaatat
      601 tttgatagga gcgtcaccta tt
//
LOCUS       DBERCA010000137          619 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.138, whole genome shotgun sequence.
ACCESSION   DBERCA010000137 DBERCA010000000
VERSION     DBERCA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.138"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            82..561
                     /locus_tag="ACL719_002010"
     CDS             82..561
                     /locus_tag="ACL719_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGS0699891.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          240 a           77 c          145 g          157 t
ORIGIN      
        1 ggagctaaag ggaacagaat ggagaaaatg gaacaaatgt gttttctaat ctgttagact
       61 gtatctagaa aggggaaaat tatgattaaa gaattgtatg aagaagtcca agggactgtg
      121 tataagtgta gaaatgaata ttaccttcat ttatgggaat tgtcggattg ggaccaagaa
      181 ggcatgctct gcttacatga attgattagt agagaagaag gactggtaga cgatattcca
      241 cgtttaagga aatatttcaa aaccaagttt cgaaatcgaa ttttagacta tatccgtaag
      301 caggaaagtc agaagcgtag atacgataaa gaaccctatg aagaagtggg tgagatcagt
      361 catcgtataa gtgagggggg tctctggcta gatgattatt atctctttca tgaaacacta
      421 agagattata gaaacaaaca aagtaaagag aaacaagaag aactagaacg cgtcttaagc
      481 aatgaacgat ttcgagggcg tcaaagagta ttaagagact tacgcattgt gtttaaggag
      541 tttactatcc gtacccacta gtaagtcatg caaaaaaaat gaaaaaaatt agaaaaagta
      601 gttgacaaag tttgaaaag
//
LOCUS       DBERCA010000138          593 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.139, whole genome shotgun sequence.
ACCESSION   DBERCA010000138 DBERCA010000000
VERSION     DBERCA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.139"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..569)
                     /locus_tag="ACL719_002011"
     CDS             complement(<1..569)
                     /locus_tag="ACL719_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS0699892.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYS"
BASE COUNT          212 a           97 c           77 g          207 t
ORIGIN      
        1 gaatacgaat cgtccttgaa attcgtgtca attatgtatc tattagctat aggtactaat
       61 gattgtatta catacaataa tgctgttaca aaaaataaaa tccctagctt attcctatac
      121 ttaaaaaaat actttaaaac aggcgacttc ttattatctt tacgagacat aaagctatct
      181 aactttttaa atgttaaaat gatatttgaa taatgccttt caaattcact tattgacatt
      241 ctctgcctgc cttttgcagg atctaaaata taaaacttgt ttttactaat tttttcaaga
      301 ataataaaat gagaactctc ccagaagcaa atgagtggga aactgagcgg ttttaaatct
      361 cttaccgaag ctttaaaagc atgagaactt atattaaaat ctcctaaaac tttacttata
      421 gataggagag aatatccaga tctggatgat gggtagattt cacgtagttc atttaaagaa
      481 atctcatatt taaaatagtt caatatcatg gcacagcatg ctaatccaca ttctgaattt
      541 tccatttgat atatcattgg aattttcaca atacttcctc cttgttcaaa ata
//
LOCUS       DBERCA010000139          515 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.141, whole genome shotgun sequence.
ACCESSION   DBERCA010000139 DBERCA010000000
VERSION     DBERCA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.141"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            103..285
                     /locus_tag="ACL719_002012"
     CDS             103..285
                     /locus_tag="ACL719_002012"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0699893.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            230..>515
                     /locus_tag="ACL719_002013"
     CDS             230..>515
                     /locus_tag="ACL719_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGS0699894.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAAVFCIHES"
BASE COUNT          204 a           92 c           88 g          131 t
ORIGIN      
        1 aaagaaatta actgagtaaa aactctcgaa attcaattcg ctatattatc actcaacttg
       61 aacaaatacc agaaccacaa aaaacagatg taaaggaaaa atatggactt aaaatttgaa
      121 ggggttgatt tagaatacaa aaaagctaaa aacaatcttc cagagtcttt ctgggaaacc
      181 tactcagcat ttgcaaatac aaatggcggg aaaataattc tcgggataga tgaaaaaaac
      241 attgacacct accaaagagt caatagactc cctgcgaaat tatgaagcaa gtaaacaatt
      301 aactgataca cgatttaagc gtcttgttgg tgttcagcgt accactttta aagagatgtt
      361 agctgtatta aaaacagctt atcaacttaa acacgcaaaa ggtggacgaa aacctaaatt
      421 aagtctagaa gaccttctta tggccactct tcaatatgtg cgagaatacc gcacttatga
      481 agaaattgcg gctgtttttt gtatccacga aagca
//
LOCUS       DBERCA010000140          498 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.142, whole genome shotgun sequence.
ACCESSION   DBERCA010000140 DBERCA010000000
VERSION     DBERCA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.142"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..261
                     /locus_tag="ACL719_002014"
     CDS             <1..261
                     /locus_tag="ACL719_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0699895.1"
                     /translation="TDIVHNLAEKVVVVRLEKPVTFHNMIAPGKEVEVSLLFFIINNS
                     SSSQTNILAQLMDFFTGNGYLEDLSKISEPEKLYAYIAEATA"
     gene            299..>498
                     /locus_tag="ACL719_002015"
     CDS             299..>498
                     /locus_tag="ACL719_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS0699896.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPG"
BASE COUNT          165 a           89 c           97 g          147 t
ORIGIN      
        1 acagatattg ttcacaatct ggctgaaaaa gttgtggtcg ttcgattaga gaaaccggtg
       61 actttccata atatgattgc gccaggtaag gaagtagaag tatccttgct cttctttatc
      121 atcaataatt caagttcaag tcaaacaaat attctggctc agttgatgga ctttttcaca
      181 ggaaatggtt atcttgaaga cctatcaaaa atttctgaac cagaaaaact ttatgcttac
      241 attgctgaag caaccgctta atcttgtcta ttaaaaaata aaatcggagg aaatccaaat
      301 gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc
      361 tctagaagaa gaactttcaa accgtggtta tgatgttcac tgtgatgcag tcatggtgaa
      421 agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga
      481 ccttggtttt gaaccagg
//
LOCUS       DBERCA010000141          457 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.143, whole genome shotgun sequence.
ACCESSION   DBERCA010000141 DBERCA010000000
VERSION     DBERCA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.143"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(77..>457)
                     /locus_tag="ACL719_002016"
     CDS             complement(77..>457)
                     /locus_tag="ACL719_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS0699897.1"
                     /translation="PREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGS
                     MAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIA
                     YTFLRYIALNQNDKKIVIPSLLEI"
BASE COUNT          152 a           88 c           62 g          155 t
ORIGIN      
        1 aactagtcaa tcttggtatt tttatattgc ttaatgagtg gacacctcta ttttagaaac
       61 aaaactataa attaagctag atttcaagta atgaggggat aactatcttt ttgtcattct
      121 gattcagtgc gatatacctt aaaaaagtat aagcaatacc agtcacacct gtatacaaag
      181 aaaaatctgg gaaattgctt gtttggacga tacgatactc tccttctttt gatttattca
      241 ttacaacact acacaataaa gactccaatt ccatactagt atccatttct ttcatgtagt
      301 cgatgtaaaa atttattatg gccatacttc catggcaaaa tgtatcatta tctaaactag
      361 ctacaattcc ctctggaaca ctttggggat gattaactaa tgtcccaaat tctccactac
      421 accacttcaa agaatgaatt ttgattttct ccctagg
//
LOCUS       DBERCA010000142          436 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.144, whole genome shotgun sequence.
ACCESSION   DBERCA010000142 DBERCA010000000
VERSION     DBERCA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.144"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..436
                     /locus_tag="ACL719_002017"
                     /pseudo
     CDS             1..436
                     /locus_tag="ACL719_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           82 c           96 g          112 t
ORIGIN      
        1 aaattgcggc tgattttggt attcacgaaa gcaacttaat ccgtcggagc caatgggttg
       61 aagtaactct tgttcaaagt ggtgttacga tttcaagaac tcctctcagt tctgaggaca
      121 cggtaatgat tgatgcgacg gaagtacaaa tcaatcgccc taaaaaaaga attagcgaat
      181 cattctggta aaaagaaatt tcacgctatg aaggctcaag cgattgtcac aagtcaaggg
      241 agaattgttt ctttggatat cgctgtgaac tatagtcatg atatgaagtt gttcaaaatg
      301 agttgcagaa atatcggaca agctggtaaa atcttggccg acagtggtta tcaagggctc
      361 atgaagatat atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaaca
      421 gctgaagata aagcct
//
LOCUS       DBERCA010000143          375 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC3 isolate 465343
            SAMN38107185-rid24769763.denovo.147, whole genome shotgun sequence.
ACCESSION   DBERCA010000143 DBERCA010000000
VERSION     DBERCA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107185
            Sequence Read Archive: SRR26673628
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570849.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 15:42:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,018
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,841
            CDSs (with protein)               :: 1,841
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 61 of 141
            Pseudo Genes (internal stop)      :: 42 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107185-rid24769763.denovo.147"
                     /strain="GPSC3"
                     /isolate="465343"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>375
                     /locus_tag="ACL719_002018"
     CDS             <1..>375
                     /locus_tag="ACL719_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGS0699898.1"
                     /translation="KKDAHGIALKLLSDPNREQFQHDNRQVELKILTRHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTAS
                     KIQDILSVAPRSIGTTSPAREF"
BASE COUNT          124 a           85 c           64 g          102 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactgac tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgga aagcattcag aatataccta ccaactcttg acacgctacc
      241 ctaatcctca gaaaaggatt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa aattcaggat atcctctcag tcgcacctcg ttctatcgga acaacatctc
      361 ctgctcgtga attcg
//
